LOCUS       DATXIG010000001        92075 bp    DNA     linear   BCT 28-DEC-2023
DEFINITION  TPA_asm: Streptococcus pyogenes strain MGAS11113
            SAMN08555799-rid19682583.denovo.001, whole genome shotgun sequence.
ACCESSION   DATXIG010000001 DATXIG010000000
VERSION     DATXIG010000001.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN08555799
            Sequence Read Archive: SRR6740495
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pyogenes
  ORGANISM  Streptococcus pyogenes
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 92075)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 92075)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-OCT-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-OCT-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001918058.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001918058.2
            Genome Coverage       :: 368x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/12/2023 11:23:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 1,806
            CDSs (total)                      :: 1,769
            Genes (coding)                    :: 1,722
            CDSs (with protein)               :: 1,722
            Genes (RNA)                       :: 37
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1, 1 (5S, 23S)
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 47
            CDSs (without protein)            :: 47
            Pseudo Genes (ambiguous residues) :: 0 of 47
            Pseudo Genes (frameshifted)       :: 30 of 47
            Pseudo Genes (incomplete)         :: 20 of 47
            Pseudo Genes (internal stop)      :: 9 of 47
            Pseudo Genes (multiple problems)  :: 11 of 47
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..92075
                     /organism="Streptococcus pyogenes"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN08555799-rid19682583.denovo.001"
                     /strain="MGAS11113"
                     /serotype="Emm28"
                     /isolation_source="normally sterile site"
                     /host="Homo sapiens"
                     /db_xref="taxon:1314"
                     /country="Finland"
                     /collection_date="2002"
                     /collected_by="National Public Health Laboratory"
     gene            127..2325
                     /gene="nrdD"
                     /locus_tag="VC122_000001"
     CDS             127..2325
                     /gene="nrdD"
                     /locus_tag="VC122_000001"
                     /EC_number="1.17.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011529096.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anaerobic ribonucleoside-triphosphate reductase"
                     /protein_id="HEP5639965.1"
                     /translation="MVSLEEDKVTVQPDIKVIKRDGRLVNFDSTKIYSALLKASMKVT
                     RMSPLVEAKLEAISDRIIAEIIERFPTNIKIYEIQNIVEHKLLAANEYAIAKEYINYR
                     TQRDFARSQATDINFSIDKLINKDQTVVNENANKDSDVFNTQRDLTAGIVGKSIGLKM
                     LPSHVANAHQKGDIHYHDLDYSPYTPMTNCCLIDFKGMLANGFKIGNAEVESPKSIQT
                     ATAQISQIIANVASSQYGGCTADRIDEFLAPYAELNFKKHMADAKKWIVETKRESYAF
                     EKTQKDIYDAMQSLEYEINTLFTSNGQTPFTSLGFGLGTSWFEREIQKAILTIRINGL
                     GSEHRTAIFPKLIFTVKRGLNLEPDSPNYDIKTLALECATKRMYPDMLSYDKIIDLTG
                     SFKSPMGCRSFLQGWKDENGQDVTSGRMNLGVVTLNLPRIAMESNGDMDKFWELFNER
                     MLISKDALIYRVERVTEAKPANAPILYQYGAFGKRLEKTGNVNDLFKNRRATVSLGYI
                     GLYEVASVFYGGQWEGNPDAKAFTLSIIKAMKQACEDWSDEYGYHFSVYSTPSESLTD
                     RFCRLDTEKFGIVTDITDKEYYTNSFHYDVRKSPTPFEKLDFEKDYPEAGASGGFIHY
                     CEYPVLQQNPKALEAVWDYAYDRVGYLGTNTPIDKCYNCQFEGDFTPTERGFTCPNCG
                     NNDPKTVDVVKRTCGYLGNPQARPMVNGRHKEISARVKHMNGSTIKYPGL"
     gene            2507..2653
                     /locus_tag="VC122_000002"
     CDS             2507..2653
                     /locus_tag="VC122_000002"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011106921.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEP5639966.1"
                     /translation="MGKYQLDYKGMQQVERFHEKHSKKKTDKKSRVQELKARFLEKSK
                     KQDN"
     gene            2682..3617
                     /locus_tag="VC122_000003"
     CDS             2682..3617
                     /locus_tag="VC122_000003"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002991347.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HEP5639967.1"
                     /translation="MLNIGIVGLGAISQKAYLPYMRQLSDITWHLSTRNAAVRQQVGQ
                     LFGHAILYSDVKELSKASLDGVFIHAATSAHAELASLFLNQGIPVFMDKPIADNYLMT
                     KNLYDLAKENQTFLMAGFNRRFTPRVKKLSSLSTKRKVAVEKNDLNRPGDMTFKLFDF
                     FIHPLDTALFLSEGTLLKGHFQYHLEAGLLSQVMVTLMTESMTTTASMNLQSGSRREV
                     MEVQRAEETYHLENLDELSIYKGTEKRVLGFASWDTTLHKRGFETMIDAFLEAISTGV
                     NPVSPESSLLSHWICQQIADSQLSYGELTVELPKD"
     gene            3626..4135
                     /locus_tag="VC122_000004"
     CDS             3626..4135
                     /locus_tag="VC122_000004"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011285262.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HEP5639968.1"
                     /translation="MEIRRPTLKDKDAVLSMINEFLEQKSATDGLWHFNVNDFNYETW
                     LEDSLRQEMGLSSQGVPAIQYVAFDERSQAIGFLNLRLRLNERLLEKGGHIGYSVRPS
                     QRGKGYAKEMLKQAVSYAISKNITTILVTCDETNVASRAVIVANGGVLEDSRGGTERY
                     WIEEQRGDK"
     gene            4135..4749
                     /gene="nrdG"
                     /locus_tag="VC122_000005"
     CDS             4135..4749
                     /gene="nrdG"
                     /locus_tag="VC122_000005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002982219.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anaerobic ribonucleoside-triphosphate reductase
                     activating protein"
                     /protein_id="HEP5639969.1"
                     /translation="MAEKCWNNPKPKEWQAEELSQGRIIDYKAFNFVDGEGVRNSLYV
                     SGCLFHCKGCYNAATWSFKAGMPYTQELEEQIMTDLAQPYVQGLTLLGGEPFLNTGIL
                     IPLIKRIRRELPEKDIWSWTGYTWEEMMLETPDKLEMLSLIDILVDGRFDITKKNLML
                     QFRGSSNQRIIDVQKSLAAKEVIIWDKLNDGDQTFEQISREDLL"
     gene            complement(4923..5654)
                     /gene="yaaA"
                     /locus_tag="VC122_000006"
     CDS             complement(4923..5654)
                     /gene="yaaA"
                     /locus_tag="VC122_000006"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012767637.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peroxide stress protein YaaA"
                     /protein_id="HEP5639970.1"
                     /translation="MLTFLIPTAKEMMIPKESHPHLLPQDSQAILKIMAAMTTEDLAK
                     SYRIKEEAAKKEQQRWQDMASQQNLAYPAYQLFNGLMYRHIKRDKLTTQEQAYLTQQV
                     YITSSFYGIIPANHPIAEHRHDFHTRIKIEGQSLKSYWRPCYNQFAKEHPQVISLLSS
                     EFDDVFSKDCKQLWISPKFMAEKEGQFKTHSTISKKARGAFLTACMENNCQTVDSLKS
                     LVFAGFYYHPDLSTDHEFVYIKKEA"
     gene            complement(5696..6553)
                     /locus_tag="VC122_000007"
     CDS             complement(5696..6553)
                     /locus_tag="VC122_000007"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002982226.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HEP5639971.1"
                     /translation="MTYPYNSTISLGTVSLNVTDLAKMTTFYTSIIGLQVLSQDTTSR
                     QLTTDGKTVILELRQTPLPANKAYGLYHTSFLVPDRHSLGLVLNHFLTRGISLEGSAD
                     HGYSEAIYLSGPEGNGIEIYHDKAVEHWDIRDNGQIIGVTEPTDTKSILEQLTDIPKR
                     FLLAQDTRIGHVHLSVKNALASSLLYQKVFDLGDKMTIPSASWIASGNYHHHLAFNHW
                     SAPYLKKHQEGAPGLAFLTIHIETPLLFSATLKKARLHGLAILQEDSSSFTTEDEEGI
                     CVNVILGQD"
     gene            6740..7077
                     /locus_tag="VC122_000008"
                     /pseudo
     CDS             6740..7077
                     /locus_tag="VC122_000008"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011018307.1"
                     /note="frameshifted; internal stop; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
     gene            complement(7356..8069)
                     /gene="treR"
                     /locus_tag="VC122_000009"
     CDS             complement(7356..8069)
                     /gene="treR"
                     /locus_tag="VC122_000009"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012561066.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="trehalose operon repressor"
                     /protein_id="HEP5639972.1"
                     /translation="MTKYERIYKDLETKINKDFYKEGDFLPTEIELSQQYQASRDTVR
                     KALSLLTKAGLILKKQGRGTQVIKHHQIMFPISELTSYQELVSYSNLDSKTNVIAIDK
                     LIVDETLSKLTGFSKNSLVWRVTRQRVVEGVASVLDIDYLSKTLVPIMTREIAEHSIY
                     QYLEKELHLAIDFALKEVTIDQITDRDKILLDLGSDQHVVSVKSKVYLSNNNQFQFTE
                     SRHKLEKFKFLDFARRRPK"
     gene            8280..10304
                     /gene="treP"
                     /locus_tag="VC122_000010"
     CDS             8280..10304
                     /gene="treP"
                     /locus_tag="VC122_000010"
                     /EC_number="2.7.1.201"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011889218.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS system trehalose-specific EIIBC component"
                     /protein_id="HEP5639973.1"
                     /translation="MGKFEQDAKSLLTAIGGKENIKAVTHCATRMRFVLNDNNKANVK
                     EIEKISVVKGTFTNAGQFQVIIGNDVPVFYNDFTAVSGIEGVSKEAAKSAAKSNQNAL
                     QQVMTMLAEIFTPIIPAIIVGGLILGFRNILESVPFEFLGQQVEKGKLVFDAAGDPVW
                     NTIVRVSPFWSGVNHFLWLPGEAIFHFLPVGITWSVTRKMGTTQILGTVLGICLVSPQ
                     LLNAYAVAGTPAAEIAKNWVWDFGFFTINRIGYQAQVIPALLAGLSLAYLEIFWRKRI
                     PEVVSMIFVPFLSLIPALILAHTVLGPIGWTIGKGISFVVLAGLTGPVKWLFGAIFGA
                     LYAPLVITGLHHMTNAIDTQLIADTATRTTGLWPMIALSNIAQGSAVFAYYLMNRHEE
                     REAEISLPAAISAYLGVTEPALFGVNVKYVYPFVAGMIGSGIAGLLSTTFNVQANSIG
                     VGGLPGFMAINVKYMIPFFICMVVAIVVPMFLAFFFRKSHIMTKTEDEAKLPETPVSD
                     APVATAPHKTMQGTVITLTSPLTGEVKALSEAVDPVFAQGVMGQGALLQPTEGVLVAP
                     CDAEVSVLFPTKHAICLVTTEGLELLMHIGMDTVNLDGQGFEALVKQGDQVKAGQTLI
                     QFDIAAISEAGYATETPLVVTNQDAFTVTVEGSLPRQIKANDKLAVAMKK"
     gene            10372..12000
                     /gene="treC"
                     /locus_tag="VC122_000011"
     CDS             10372..12000
                     /gene="treC"
                     /locus_tag="VC122_000011"
                     /EC_number="3.2.1.93"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015058125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha,alpha-phosphotrehalase"
                     /protein_id="HEP5639974.1"
                     /translation="MTIDKKKVVYQIYPKSYKDTTGNGVGDLRGIIDKLPYLQELGID
                     MIWLNPFYPSPQRDNGYDVSDYTAVNPDFGTMADFEDLVKAAKEHQIELMLDMVLNHC
                     STDHEWFQKALAGDPYYQDFFILRDQPTDWVSKFGGNAWAPFGDTGKYYLHLFDVTQA
                     DLNWRNPHVREELAKVVNFWRDKGVKGFRFDVINLIGKDEELVDCPVNDGKPAYTDRP
                     ITHTYLHDLNQASFGQDDSFMTVGEMSATTIDNCLLYTAPEREELSMAFNFHHLKVDY
                     ENGQKWTIMAFDFAALRDLFHAWGEGMSQGNGWNALFYNNHDQPRALNRFVDVTHFRN
                     EGATMLAASIHLSRGTPYIYMGEEIGMLDPDFDSMDDYVDVESLNAYSSLLVSGKSAE
                     EAFAIIKAKSRDNARTPMQWDASEHAGFTTGKPWLEVGKSYRDINVETEKEGRIFPFY
                     QRLIALRKELPIIAEGDYRAAFKDSQAVYAFERHLGDQCLLVLNHFYAGEVELELPPR
                     YQHGQALISNYEKVSICEKVMLKPYQTLAILADN"
     gene            12208..14103
                     /gene="pepO"
                     /locus_tag="VC122_000012"
     CDS             12208..14103
                     /gene="pepO"
                     /locus_tag="VC122_000012"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010922744.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="endopeptidase PepO"
                     /protein_id="HEP5639975.1"
                     /translation="MTTYQDDFYQAVNGKWAETAVIPDDKPRTGGFSDLADEIEALML
                     DTTDAWLAGDHIPDDAILKNFVKFHRLVADYAKRDEVGVSPILPLIEEYQSLKSFSEF
                     VANIAKYELAGLPNEFPFSVAPDFMNAQLNVLWAEAPSILLPDTTYYEEGNEKAEELR
                     GIWRQSQEKLLPQFGFSTEEIKDLLDKVIELDKQLAKYVLSREEGSEYAKLYHPYVWA
                     DFKKLAPELPLDSIFEKILGQVPDKVIVPEERFWTEFAATYYSEANWDLLKANLIVDA
                     ANAYNAYLTDDIRVESGAYSRALSGTPQAMDKQKAAFYLAQGPFSQALGLWYAGQKFS
                     PEAKADVESKVARMIEVYKSRLETADWLAPATREKAITKLNVITPHIGYPEKLPETYA
                     KKVIDESLSLVENAQNLAKITIAHTWSKWNKPVDRSEWHMPAHLVNAYYDPQQNQIVF
                     PAAILQEPFYSLDQSSSANYGGIGAVIAHEISHAFDTNGASFDEHGSLNDWWTQEDYA
                     AFKERTDKIVAQFDGLESHGAKVNGKLTVSENVADLGGVACALEAAQSEEDFSARDFF
                     INFATIWRMKAREEYMQMLASIDVHAPGELRTNVTLTNFDAFHETFDIKEGDAMWRAP
                     KDRVIIW"
     gene            complement(14269..15309)
                     /locus_tag="VC122_000013"
     CDS             complement(14269..15309)
                     /locus_tag="VC122_000013"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003053075.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor Ts"
                     /protein_id="HEP5639976.1"
                     /translation="MAEITAKLVKELREKSGAGVMDAKKALVETDGDMDKAVELLREK
                     GMAKAAKKADRVAAEGLTGVYVHGNVAAVVEVNAETDFVAKNAQFVELVNATAKVIAE
                     GKPANNDEALALVMPSGETLAEAYVNATATIGEKISFRRFALIEKTDEQHFGAYQHNG
                     GRIGVISVVEGGDDALAKQVSMHIAAMKPTVLSYTELDAQFIKDELAQLNHAIELDNE
                     SRAMVDKPALPFLKYGSKAQLSDDVITAAEADIKAELAAEGKPEKIWDKIIPGKMDRF
                     MLDNTKVDQAYTLLAQVYIMDDSKTVEAYLDSVNAKAIAFARFEVGEGIEKKANDFES
                     EVAATMAAALNN"
     gene            complement(15443..16210)
                     /gene="rpsB"
                     /locus_tag="VC122_000014"
     CDS             complement(15443..16210)
                     /gene="rpsB"
                     /locus_tag="VC122_000014"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011285255.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S2"
                     /protein_id="HEP5639977.1"
                     /translation="MAVISMKQLLEAGVHFGHQTRRWNPKMAKYIFTERNGIHVIDLQ
                     QTVKLADQAYEFVRDAAANDAVILFVGTKKQAAEAVADEATRAGQYFINHRWLGGTLT
                     NWGTIQKRIARLKEIKRMEEEGTFDVLPKKEVALLNKQRARLEKFLGGIEDMPRIPDV
                     MYVVDPHKEQIAVKEAKKLGIPVVAMVDTNADPDDIDIIIPANDDAIRAVKLITAKLA
                     DAIIEGRQGEDADVAFEADIQADSIEEIVEVVEGDNA"
     gene            16514..19588
                     /locus_tag="VC122_000015"
     CDS             16514..19588
                     /locus_tag="VC122_000015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014635774.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HEP5639978.1"
                     /translation="MTKGIRFQLLGSPHIFLDDKEQFFAFAKANALLYYLVVNGSVSR
                     EVAASLLWENKNTQTAKKNLRNAIYQVNKVLQADVIICPNRNLLVLKKTLDIKTDINL
                     FLADPLAHLELYQGEFLQGFYLKSGEEFDLWVSKMRMQYEQVYLKACYQKIEEKLSLD
                     AIEDVEEHLKQLIERDEFEEKNYQLLMRLYQQGNCPGKVIETYYQLANVLDKELGIQP
                     SLQSQQIYQEVVAKDRNERKIKHFLRNSNHFLGRIDEIKQLENYFANCLACQEVGALL
                     LIGDTGIGKRTLARQVLANQTQTFQIVTAKCFREEAMDSLLPWRNILDGLGDLVIQNR
                     LLTTKAWKAALKRCFPVATIFQEDNNQPFIKDHTSLLVSFIVDILQHLAEIKALVILI
                     EDCHWMDEDSLTLLQRVMNQLVHYPIAFVLTKHLGTTPELGLCLNALMSQGRLESICL
                     EPFNRQESLAYINSQLGSQPVTEEEMEHLYQASQGNPFFLSEYTQTLLRHEKFVPLTP
                     AIKAKLGLKLANLSSRDDALLNYLSCCRRPIPLNTLAQLMLLPLEEVIEMVDNLGHYY
                     ILVEESVGEEVLISFRQRIIQLYSYDRLSLSKRRLLHGQIAKRLEDLLPILTPSPHLL
                     DDIAYHYQESRQVIKALEYNLNYLDATLPFQHELFPIYSKSIGSLEKSDRDHQRLMEE
                     QFDRIRQSIADLELTYDNNRDFQQLLIRFSYLEGRYDIRTGRYQEGIKNIQKVIALAT
                     ELKQPSFLLEGYRQLIHYCIQVENKPEMRYYTSLSLEAAVAANHFEAIAISLRLNGLY
                     HLIIGELNEAERLLQQSIDFFKVTPGLQANYAIQIAAALDYLGEIAQIRYQFEKAVAY
                     QKQAIALTENKPAELSVSIFYIGLGISYFYLADFEQAEQILSLAKEALVNHSYPWKET
                     QLQIYLAMIQWKKGNYQPALTLLDYRETLMSRYRNPRDKGLVFYLMAVVKYQLIFQGA
                     TLSQQEKEMADYLLSESFEYYYEIASTNLNPYRDCHLVSELNDLRQQLSAKS"
     gene            complement(19619..20605)
                     /locus_tag="VC122_000016"
     CDS             complement(19619..20605)
                     /locus_tag="VC122_000016"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014635773.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formimidoylglutamase"
                     /protein_id="HEP5639979.1"
                     /translation="MLEDYYPSTTSYYHGGIDDDLYTAKWGMVMTFLDLNDSSLTPFE
                     GTHFALIGFKSDKGVYINNGRVGAVESPAAIRTQLAKFPWHLGNQVMVYDVGNIDGPN
                     RSLEQLQNSLSKAIKRMCDLNLKPIVLGGGHETAYGHYLGLRQSLSPSDDLAVINMDA
                     HFDLRPYDQTGPNSGTGFRQMFDDAVADKRLFKYFVLGIQEHNNNLFLFDFVAKSKGI
                     QFLTGQDIYQMGHQKVCRAIDRFLEGQERVYLTIDMDCFSVGAAPGVSAIQSLGVDPN
                     LAVLVLQHIAASGKLVGFDVVEVSPPHDIDNHTANLAATFIFYLVQIMAQHN"
     gene            complement(20791..22332)
                     /gene="hutH"
                     /locus_tag="VC122_000017"
     CDS             complement(20791..22332)
                     /gene="hutH"
                     /locus_tag="VC122_000017"
                     /EC_number="4.3.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012130845.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine ammonia-lyase"
                     /protein_id="HEP5639980.1"
                     /translation="MTRVINLDGESLTIEDVIAIARQGVACRIDDSAIEAVNASRKIV
                     DDIVSEKRVVYGVTTGFGSLCNVSISPEDTVQLQENLIRTHASGFGDPLPEDAVRAIM
                     LIRINSLVKGYSGIRLSTIEKLLELLNKRVHPYIPEKGSLGASGDLAPLAHMVLPMLG
                     LGKAYYKGELLSGQEALDKAGIDKISLAAKEGLALINGTTVLTAIGALATYDAIQLLK
                     LSDLAGALSLEVHNGITSPFEENLHTIRPQSGQLATARNIRNLIEGSQNTTVATQSRV
                     QDPYTLRCMPQIHGASKDSIAYVKSKVDIEINSVTDNPIICKDGHVISGGNFHGEPMA
                     QPFDFLGIAISEIGNVSERRVERLVNSQLSKLPSFLVKYPGLNSGFMITQYACASLAS
                     ENKVLAHPASVDSIPSCENQEDFVSMGTTAARKAFEILKNSRRIVATEIMAACQALDL
                     KPENHELGKGTKVAYDLFRKEINFIEHDKHIEIYDELNKASAVIEDPSFLEAVEQAVE
                     LSIQF"
     gene            complement(22343..23686)
                     /locus_tag="VC122_000018"
     CDS             complement(22343..23686)
                     /locus_tag="VC122_000018"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012767629.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="APC family permease"
                     /protein_id="HEP5639981.1"
                     /translation="MASNHMNEQEREQAKFSLSGATLYGINAVIGSGIFLLPRAIYKG
                     LGPASIAVMFGTAILTIMLAVCFAEVSGYFGKNGGAFQYSKRAFGDFIGFNVGFLGWT
                     VTIFAWAAMAAGFARMFIITFPAFEGWHIPLSIGLIILLSLMNIAGLKTSKIVTITAT
                     IAKLIPIVAFCACTLFFIKNGLPNFTPFVQLEPGTNLLGAISNTAVYIFYGFIGFETL
                     SIVAGEMRAPEKNVPRALLGSISIVSVLYMLIIGGTIAMLGSQIMMTNAPVQDAFVKM
                     IGPAGAWMVSIGALISITGLNMGESIMVPRYGAAIADEGLLPAAIAKQNQNGAPLVAI
                     LVSGAIAIVLLLTGSFENLAKLSVVFRFFQYIPTALAVMKLRKDDPDANVIFRVPFGP
                     IIPILAVIVSLVMIWGDNPMNFVYGAVGVIIASSVYYLMHGRNHQMDQMPPKEQI"
     gene            complement(23906..24502)
                     /locus_tag="VC122_000019"
     CDS             complement(23906..24502)
                     /locus_tag="VC122_000019"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002992805.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HutD family protein"
                     /protein_id="HEP5639982.1"
                     /translation="MTKVTIKAPSDYLQTDWSGGETNQLFLYPEDGDYKKREFSYRLS
                     KSTVALRQTTFTLLDGYHRILMTLDKPITLSDLSHAKEISLTPFEPYAFEGDTRIVSR
                     GICQDINLMYDDQYQGQLLPVWSDTPFEAPKADCQLLYALSKLTYSVTNQANQELEAN
                     HLLVIEQEASLSDLKVCPNQTSPAQTPVAVWAGLWYKT"
     gene            complement(24524..26197)
                     /locus_tag="VC122_000020"
     CDS             complement(24524..26197)
                     /locus_tag="VC122_000020"
                     /EC_number="6.3.4.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002982280.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate--tetrahydrofolate ligase"
                     /protein_id="HEP5639983.1"
                     /translation="MVLSDIEIANSVTMEPISKVANQLGIDEEALCLYGKYKAKIDAR
                     QLVALKDKPDGKLILVTAISPTPAGEGKTTTSVGLVDALSAIGKKAVIALREPSLGPV
                     FGVKGGAAGGGHAQVVPMEDINLHFTGDFHAIGVANNLLAALIDNHIHHGNSLGIDSR
                     RITWKRVVDMNDRQLRHIVDGLQGKVNGVPREDGYDITVASEIMAILCLSENISDLKA
                     RLEKIIIGYNYQGEPVTAKDLKAGGALAALLKDAIHPNLVQTLEHTPALIHGGPFANI
                     AHGCNSVLATKLALKYGDYAVTEAGFGADLGAEKFIDIKCRMSGLRPAAVVLVATIRA
                     LKMHGGVPKANLATENVQAVVDGLPNLDKHLANIQDVYGLPVVVAINKFPLDTDAELQ
                     AVYDACDKRGVDVVISDVWANGGAGGRELAEKVVALAEQDNQFCFVYEEDDSIETKLT
                     KIVTKVYGGKGIRLTPAAKRELADLERLGFGNYPICMAKTQYSFSDDAKKLGAPTDFT
                     VTISNLKVSAGAGFIVALTGAIMTMPGLPKVPASETIDIDEEGNITGLF"
     gene            complement(26215..26841)
                     /locus_tag="VC122_000021"
     CDS             complement(26215..26841)
                     /locus_tag="VC122_000021"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002991312.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cyclodeaminase/cyclohydrolase family protein"
                     /protein_id="HEP5639984.1"
                     /translation="MGLVDLSLTDFAKVLGSDAPAPGGGSAAALSGANGISLTKMVCE
                     LTLGKKKYADYQAIITEIHAKSTALQASLLAAIDKDTEAFNLVSAVFDMPKETDEDKA
                     ARRTAMQKALKTAAQSPFEMMTLMVEALEITATAVGKSNTNAASDLGVAALNLKAGLQ
                     SAWLNVLINLSGIKDEDFVTDYRQKGQALLDKGCHLADDIYTKILDIV"
     gene            complement(26852..27751)
                     /gene="ftcD"
                     /locus_tag="VC122_000022"
     CDS             complement(26852..27751)
                     /gene="ftcD"
                     /locus_tag="VC122_000022"
                     /EC_number="2.1.2.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003005485.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate formimidoyltransferase"
                     /protein_id="HEP5639985.1"
                     /translation="MAKIVECIPNFSEGQNQAVIDGLVATAKSIPGVTLLDYSSDASH
                     NRSVFTLVGDDQSIQEAAFQLVKYASENIDMTKHHGEHPRMGATDVCPFVPIKDITTQ
                     ECVEISKQVAERINRELDIPIFLYEDSATRPERQNLAKIRKGQFEGMPEKLLEEDWAP
                     DYGDRKIHPTAGVTAVGARMPLVAFNVNLDTDNIDIAHKIAKIIRGSGGGYKYCKAIG
                     VMLEDRHIAQVSMNMVNFEKCSLYRTFETIKFEARRYGVNVIGSEVIGLAPAKALIDV
                     AEYYLQVEDFDYPKQVLENHLLG"
     gene            complement(27839..29869)
                     /locus_tag="VC122_000023"
     CDS             complement(27839..29869)
                     /locus_tag="VC122_000023"
                     /EC_number="4.2.1.49"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002991306.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="urocanate hydratase"
                     /protein_id="HEP5639986.1"
                     /translation="MSFYSETDIAAAMTVKLDDVLPEKTVFKEGIRRAPDRGFRLTQA
                     QTEIALKNALRYVPTKFHEEVIPEFLEELKTRGRIYGYRWRPKDRIYGKPINEYKGNC
                     TAAKAMQVMIDNNLSFEIALYPYELVTYGETGSVCANWMQYCLIKKYLEVMTDEQTLV
                     VESGHPVGLFQSKPEAPRVIITNGLLVGEYDNMKDWEIAEEMGVTNYGQMTAGGWMYI
                     GPQGIVHGTFNTLLNAGRLKLGVADDGDLTGKLFISSGLGGMSGAQGKAAEIAKAVAI
                     IAEVDQSRIETRHSQGWVTQIAESPEEALQLAQKAIDAKESTSIAYHGNIVDLLEYVN
                     DKQIHVDLLSDQTSCHNVYDGGYCPVGISFDERTRLLAEDKDTFHQMVDDTLARHFEA
                     IKTLTENGTYFFDYGNAFMKSVYDSGITEISKNGRNDKDGFIWPSYVEDIMGPMLFDY
                     GYGPFRWVCLSGKHEDLVATDKAAMEAIDPDRRYQDRDNYNWIRDAEKNQLVVGTQAR
                     ILYQDCIGRVTVALKFNELVRKGKIGPVMIGRDHHDVSGTDSPFRETSNIKDGSNVTC
                     DMAVQCYAGNAARGMSLVALHNGGGTGIGKAINGGFGLVLDGSERIDEIIKSAIAWDT
                     MGGVARRNWARNEHAIETAIEYNRLHAGTDHITIPYLADDDLVTSAVDQLFH"
     gene            30161..31426
                     /locus_tag="VC122_000024"
     CDS             30161..31426
                     /locus_tag="VC122_000024"
                     /EC_number="3.5.2.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008809789.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="imidazolonepropionase"
                     /protein_id="HEP5639987.1"
                     /translation="MVADVLLTHFNQLFCLNDPGHPLTGQEMKKATIVEDGYIAIKDG
                     LIVALGSGEPDAELVGPQTIMRSYKGKIATPGIIDCHTHLVYGGSREHEFAKKLAGVS
                     YLDILAQGGGILSTVRATRSASFDNLYQKSKRLLDYMLLHGVTTVEAKSGYGLDWETE
                     KRQLDVVAALEKDHPIDLVSTFMAAHAIPEEYKGNPKAYLDVIIKDMLPVVKEENLAE
                     FCDIFCEKNVFTADESRYLLSKAKEMGFKLRIHADEIASIGGVDVAAELSAVSAEHLM
                     MITDDGIAKLIGAGVIGNLLPATTFSLMEDTYAPARKMIDAGMAITLSTDSNPGSCPT
                     ANMQFVMQLGCFMLRLTPIEVLNAVTINAAYSVNRQERVGSLTVGKEADIAIFDAPNI
                     DYPFYFFATNLIHQVYKKGQLTVDRGRIL"
     gene            complement(31484..33016)
                     /gene="ahpF"
                     /locus_tag="VC122_000025"
     CDS             complement(31484..33016)
                     /gene="ahpF"
                     /locus_tag="VC122_000025"
                     /EC_number="1.8.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000242134.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alkyl hydroperoxide reductase subunit F"
                     /protein_id="HEP5639988.1"
                     /translation="MALSPDIKEQLAQYLTLLEADLVLQVSLGDNEQSQKVKDFVEEI
                     AAMSERISIENITLDRQPSFKVAKKGHGSGVVFAGLPLGHELTSFILALLQVSGRAPK
                     VDQDVIDRIKAIDRPLHFETYVSLTCHNCPDVVQALNIMSVLNDKISHTMVEGGMFQD
                     EVKAKGIMSVPTVFLDGEEFTSGRATIEQLLEQIAGPLSEEAFADKGLYDVLVIGGGP
                     AGNSAAIYAARKGLKTGLLAETFGGQVMETVGIENMIGTLYTEGPKLMAEVEAHTKSY
                     DVDIIKAQLATSIEKKENIEVTLANGAVLQAKTAILALGAKWRNINVPGEDEFRNKGV
                     TYCPHCDGPLFEGKDVAVIGGGNSGLEAALDLAGLAKHVYVLEFLPELKADKVLQDRA
                     AKTNNMTIIKNVATKDIVGEDHVTGLSYTERDSGEDKHLDLEGVFVQIGLVPNTAWLK
                     DSGVNLTDRGEIIVDKHGSTNIPGIFAAGDCTDSAYKQIIISMGSGATAAIGAFDYLI
                     RQ"
     gene            complement(33037..33597)
                     /gene="ahpC"
                     /locus_tag="VC122_000026"
     CDS             complement(33037..33597)
                     /gene="ahpC"
                     /locus_tag="VC122_000026"
                     /EC_number="1.11.1.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002273221.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peroxiredoxin"
                     /protein_id="HEP5639989.1"
                     /translation="MSLIGKEIAEFSAQAYHDGKFITVTNEDVKGKWAVFCFYPADFS
                     FVCPTELGDLQEQYETLKSLGVEVYSVSTDTHFVHKAWHDDSDVVGTITYPMIGDPSH
                     LISQAFEVLGEDGLAQRGTFIVDPDGIIQMMEINADGIGRDASTLIDKIHAAQYVRKH
                     PGEVCPAKWKEGAETLTPSLDLVGKI"
     gene            34004..34074
                     /locus_tag="VC122_000027"
     tRNA            34004..34074
                     /locus_tag="VC122_000027"
                     /product="tRNA-Cys"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:34036..34038,aa:Cys,seq:gca)
     gene            34593..34796
                     /locus_tag="VC122_000028"
     CDS             34593..34796
                     /locus_tag="VC122_000028"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002991299.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cold-shock protein"
                     /protein_id="HEP5639990.1"
                     /translation="MAQGTVKWFNAEKGFGFISTENGQDVFAHFSAIQTNGFKTLEEG
                     QKVAFDVEEGQRGPQAVNITKLA"
     gene            34992..35453
                     /locus_tag="VC122_000029"
     CDS             34992..35453
                     /locus_tag="VC122_000029"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003085318.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CtsR family transcriptional regulator"
                     /protein_id="HEP5639991.1"
                     /translation="MPTKNTSDSIEEYIKELLAKSGIAEIKRSMLADSFQVVPSQINY
                     VIKTRFTESRGYEVESKRGGGGYIRIAKVHFSDKHHLIGNLMATIEDCISEQVFTDSI
                     QLLFDEHLLTEREGNIILAVASDDVLGTDGSTIRARMLYRLLQRIDRKGSN"
     gene            35453..37897
                     /locus_tag="VC122_000030"
     CDS             35453..37897
                     /locus_tag="VC122_000030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011185037.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent Clp protease ATP-binding subunit"
                     /protein_id="HEP5639992.1"
                     /translation="MIMYSTKMQDIFRQAQFQAARFDSHCLETWHVLLAMVAVDNSLA
                     NMILSEYDAQVAIEEYEAAAILAMGKTPKEQLSRVDFRPQSKTLTNLLAFAQAISQIT
                     RDQEVGSEHVLFAILLNPDIMASRLLEIAGYQIKDNGNGQPRLADLRKAIERHAGYSK
                     EMIKAIHELRKPKKTKTQGTFSDMMKPPSTAGELSDFTRDLTEMARQGLLESVIGRDQ
                     EVSRMIQVLSRKTKNNPVLVGDAGVGKTALAYGLAQRIANGAIPYELKEMRVLELDMM
                     SVVAGTRFRGDFEERMNQIIDDIEADGQIILFVDELHTIMGSGSGIDSTLDAANILKP
                     ALSRGTLHMVGATTQEEYQKHIEKDAALSRRFAKILIEEPNTEDAYQILMGLKLSYET
                     YHNVSISNEAVKTAVKMAHRYLTSKNLPDSAIDLLDEASAAVQNMVKKSAPETLTPID
                     QALINGDMKKVSRLLAKEAKGQMRKPTPVTEDDILATLSKLSGIPLEKLTQADSKKYL
                     NLEKELHKRVIGQDAAVTAISRAIRRNQSGIRTGKRPIGSFMFLGPTGVGKTELAKAL
                     AEVLFDDEAALIRFDMSEYMEKFAASRLNGAPPGYVGYDEGGELTQKVRNKPYSVLLF
                     DEVEKAHPDIFNVLLQVLDDGILTDSRGRKVDFSNTIIIMTSNLGATALRDDKTVGFG
                     VKGIHQDHQAMEKRILEELRKTYRPEFINRIDEKVVFHSLTQDNMRDVVKIMVQPLIT
                     TLAEKGITLKIQPLALKHLSEVGYDEHMGARPLRRTLQTEIEDKLSELILSRELTSGH
                     TLKIGLSYGKLTFHIA"
     gene            38075..38365
                     /gene="groES"
                     /locus_tag="VC122_000031"
     CDS             38075..38365
                     /gene="groES"
                     /locus_tag="VC122_000031"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002991292.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="co-chaperone GroES"
                     /protein_id="HEP5639993.1"
                     /translation="MLKPLGDRVVVRFDDEKEQTVGGFVLAGTHKESTRKATVLAVSE
                     TGVRTITGDSVLPSVSVGQEVLVENGHDLEVTVDDEKVSIIRESDIIAIVTK"
     gene            38401..40032
                     /gene="groL"
                     /locus_tag="VC122_000032"
     CDS             38401..40032
                     /gene="groL"
                     /locus_tag="VC122_000032"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009880885.1"
                     /note="60 kDa chaperone family; promotes refolding of
                     misfolded polypeptides especially under stressful
                     conditions; forms two stacked rings of heptamers to form a
                     barrel-shaped 14mer; ends can be capped by GroES;
                     misfolded proteins enter the barrel where they are
                     refolded when GroES binds; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chaperonin GroEL"
                     /protein_id="HEP5639994.1"
                     /translation="MAKDIKFSADARAAMVRGVDMLADTVKVTLGPKGRNVVLEKAFG
                     SPLITNDGVTIAKEIELEDHFENMGAKLVSEVASKTNDIAGDGTTTATVLTQAIVHEG
                     LKNVTAGANPIGIRRGIETATATAVEALKAIAQPVSGKEAIAQVAAVSSRSEKVGEYI
                     SEAMERVGNDGVITIEESRGMETELEVVEGMQFDRGYLSQYMVTDNEKMVADLENPFI
                     LITDKKVSNIQDILPLLEEVLKTNRPLLIIADDVDGEALPTLVLNKIRGTFNVVAVKA
                     PGFGDRRKAMLEDIAILTGGTVITEDLGLELKDATMTALGQAAKITVDKDSTVIVEGS
                     GSSEAIANRIALIKSQLETTTSDFDREKLQERLAKLAGGVAVIKVGAPTETALKEMKL
                     RIEDALNATRAAVEEGIVAGGGTALITVIEKVAALELEGDDATGRNIVLRALEEPVRQ
                     IALNAGYEGSVVIDKLKNSPAGTGFNAATGEWVDMIKTGIIDPVKVTRSALQNAASVA
                     SLILTTEAVVANKPEPAAPAPAMPAGMDPGMMGGF"
     gene            40283..40549
                     /locus_tag="VC122_000033"
     CDS             40283..40549
                     /locus_tag="VC122_000033"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014635765.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HEP5639995.1"
                     /translation="MNHFGEIFKTFRESKGLRLKDVAKAGISTSQLSRFEKGETDLTI
                     STFMLILDESNMSIDEFMYAVHDFHRDDLNELLSKSEGFRNNSR"
     gene            complement(41272..42771)
                     /locus_tag="VC122_000034"
     CDS             complement(41272..42771)
                     /locus_tag="VC122_000034"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011889202.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="C69 family dipeptidase"
                     /protein_id="HEP5639996.1"
                     /translation="MINKKISLGVLSILTAFSLQSVSYACTGFIIGKDLTKDGSLLYG
                     RTEDLEPHHNKNFIVRLAKDNPAGEKWKDPSNGFEYPLPEHSYRYSAIPDVTPNKGVY
                     DEAGFNEFGVSMSATVSASANDAIQKIDPYVKNGLAESSMASVILPSVKTAREGVALI
                     AKIVTEKGAAEGNIVTLADKDGIWYMEILSGHQYVAIKFPDDKYAVFPNTFYLGHVDF
                     NDKENTIASEDVEKVTKKAKSYTEVDGKFHIAKSYNPPLNDANRSRSFSGIKSLDPDS
                     KVTYKDSNYELLQSTDKTFSLEDAMKLQRNRFEGLDLKPLDQMALDGKGKPKSKKAVK
                     GYAYPISNPNVMEAHIFQLKKDIPAELGGGVMWLSIGSPRNAPYLPYLGNISRTYEAY
                     QEKSTQYNDKSWYWTVSHINDLVAAHPKPFGTKVIDEMKGLEKTWIAEQDKSTKEISD
                     LVVSDPKAAQEKADKISLDRAEKTFKRLKAIEAKLVEEKPQNKKGLNRS"
     gene            43018..44658
                     /locus_tag="VC122_000035"
     CDS             43018..44658
                     /locus_tag="VC122_000035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011055066.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEP5639997.1"
                     /translation="MTKPFHHKKLKQITIIAATSLFLFLIGGAFYYSKNHCINAYLKA
                     RSAQSGPVFENIKAYLVWDDTNEQITNDEAMYTKFRRYSQKELRQKKQDLKAASQDSA
                     VQVKSVGRRFWIFPDYRIAIKPMDLTIKTNVPQADVLLNQKKVAVSDSEQFSVKLDRL
                     PTAEYTASIRGKHNGRNIKVNKSYDGDNPVLDLSVSFRTFLVTSNAKQGDLYFDDNHI
                     GTLKDGQLQVEDYPVTENAQAYMKTTFPDGELRSQKYALADVEEGATLEILVTDLLEE
                     DKAGELLVSAFDQLMHYLSTGQDSSNLRSVFEAGSSNAFYRGLKESIKAKFQTDTRKA
                     SRLNIPSILLTTMTQVGKTTYVLDFTATYEFLYDNSTDPEQHTSGHINQDLTGKVTVK
                     KVGQHYLISQSGSKNITVVKEDNQLKAPSVFPESILGTWTGQANGLSIHMSLASDGTI
                     TTKVEDRKGNRSKETRTAKISKVEDKGNGFYLYTPDPGSDISALAPEGGLGGANVKYA
                     YGFKISGKTASPVVWQAALTHEFDYTKPLSGVTLQKQP"
     gene            complement(44747..45748)
                     /locus_tag="VC122_000036"
     CDS             complement(44747..45748)
                     /locus_tag="VC122_000036"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011285752.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RluA family pseudouridine synthase"
                     /protein_id="HEP5639998.1"
                     /translation="MTSVKVLKLSLLLLPPKWLSTLLKNTITGSMKTTNKPSKGFTVT
                     LKNPFEELTVKALLEEQLLIPRKIRHFLRTKKHVLINGHPVNWQSCVKYGDQVKLFFD
                     HEDYPEKIIVMGQAEKVTCLYEDEHIIIVNKPEGMKTHGNDPTELALLNHVSAYTGQT
                     CYVVHRLDKETSGAILFAKTPFILPILNRLLEKRDIHREYLALVHGSLDSPRVTYHHP
                     IGRHRHDRRKRVVDPINGKKAITEVTLVKNFHKTASLLNCQLQTGRTHQIRVHLAHQG
                     HVLFGDPLYSNGKKDCARLMLHAYQLRLKHPLTQEDICVQAKSATFDAVLNAQKARK"
     gene            46325..46705
                     /locus_tag="VC122_000037"
     CDS             46325..46705
                     /locus_tag="VC122_000037"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002982339.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RidA family protein"
                     /protein_id="HEP5639999.1"
                     /translation="MKTIRRYDVNEDRGHTGLVEAGDFYYLNYCVGNVGQDIESQING
                     AFDEMERRLALVGLTLDAVVQMDCLFRDVWNIPVMEKMIKERFNGRYPARKSIQTEFA
                     HHGGPQGLLFQVDGVAYSKHISMT"
     gene            46744..49080
                     /locus_tag="VC122_000038"
     CDS             46744..49080
                     /locus_tag="VC122_000038"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002982341.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin-binding protein"
                     /protein_id="HEP5640000.1"
                     /translation="MRFLELLQKKFFPKAYQEKQFLMHQKTRLTPQHNQKQYSPNANH
                     LDSSATKNSEQDPAIALQRSRAYEGSPKSRPAWLQKLEAVLPSPQHPIRRFWRRYHIG
                     KLLMILIGTLVLLLGSYLFYLSKTAKVSDLQDALKATTVIYDHKGEYAGSLSGQKGSY
                     VELNAISDDLENAVIATEDRTFYSNSGINLKRFLLAVVTAGRFGGGSTITQQLAKNAY
                     LSQDQTIKRKAREFFLALELTKKYSKKDILTMYLNNSYFGNGVWGVEDASQKYFGTTA
                     ANLTLDEAATLAGMLKGPEIYNPYHSLKNATHRRDTVLGAMVDAKKITQTKAQQARAV
                     GLKNRLADTYVGKTDDYKYPSYFDAVISEAIATYGLSEKDIVNNGYKVYTELDQNYQT
                     GMQTTFNNDELFPVSAYDGSSAQAASVALDPKTGGVRGLIGRVNSSENPTFRSFNYAT
                     QAKRSPASTIKPLVVYAPAVASGWSIEKELPNTVQDFDGYQPHNYGNYESEDVPMYQA
                     LANSYNIPAVSTLNDIGIDKAFTYGKTFGLDMSSAKKELGVALGGSVTTNPLEMAQAY
                     AAFANNGVIHPAHLINRIENARGEVLKTFTDKAKRVVSQSVADKMTAMMLGTFSNGTA
                     VNANVYGYTLAGKTGTTETNFNPDLAGDQWVIGYTPDVVISQWVGFNQTDENHYLTDS
                     SAGTASAIFSTQASYILPYTKGSQFHVDNAYAQNGISAVYGVNETGNQSGVDTQSIID
                     GLRKSAQEASQSLSKAVDQSGLRDKAQSIWKEIVDYFR"
     gene            49129..49281
                     /gene="rpmG"
                     /locus_tag="VC122_000039"
     CDS             49129..49281
                     /gene="rpmG"
                     /locus_tag="VC122_000039"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015912025.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L33"
                     /protein_id="HEP5640001.1"
                     /translation="MAQKKASLACVECGSRNYSIGVSSTPKPTRLEVNKFCKYCKTYT
                     LHKETR"
     gene            49295..49471
                     /gene="secE"
                     /locus_tag="VC122_000040"
     CDS             49295..49471
                     /gene="secE"
                     /locus_tag="VC122_000040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002982348.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecE"
                     /protein_id="HEP5640002.1"
                     /translation="MGFISGTFKVLKDTTWPNRKQRWKDFISVLEYTAFFTVIIYIFD
                     QLLAKSVLALINLF"
     gene            complement(49708..50481)
                     /locus_tag="VC122_000041"
     CDS             complement(49708..50481)
                     /locus_tag="VC122_000041"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003059847.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycyl-radical enzyme activating protein"
                     /protein_id="HEP5640003.1"
                     /translation="MTDRGIVFNIQHFSIHDGPGIRTTVFLKGCPLRCPWCANPESQQ
                     KAPEQMLTSDGLNTKIVGEEKTVDEVIEEVLKDLDFYEESGGGMTLSGGEIFAQFDFA
                     LALLKAAKAAGLHTAIETTAFAKHEQFVTLIDYVDFIYTDLKHYNQLRHQKVTGVRND
                     LIIKNIHYAFQAGKEIVLRIPVIPQFNDSLDDAKAFSELFNQLEIDQVQLLPFHQFGE
                     NKYKLLGREYEMAEVKAYHPEDLADYQAVFLNHNIHCYF"
     gene            50607..51353
                     /locus_tag="VC122_000042"
     CDS             50607..51353
                     /locus_tag="VC122_000042"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011889198.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DeoR/GlpR transcriptional regulator"
                     /protein_id="HEP5640004.1"
                     /translation="MNRLERIIQLVSQKKKIDVNSLSEQLEVSKVTIRKDLDKLESKG
                     LLRREHGYAVLNSGDDLNVRLSYNYNIKRRIAEKAAELVQDNDTIMIESGSTCALLAE
                     VLCQTKRNIKIITNSCFIANYIRQYSSCQIILLGGYYQPNSEVTVGPLLKEMISLFHV
                     NRVFVGTDGFNKDLGFMGKDMMRSEGVRYMADAAEEVVILTDSSKFSKTSLVHQLSLA
                     DVNRVITDQALDKQTQELLSSGGLVLDFVS"
     gene            51369..52349
                     /locus_tag="VC122_000043"
     CDS             51369..52349
                     /locus_tag="VC122_000043"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002982359.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar-binding transcriptional regulator"
                     /protein_id="HEP5640005.1"
                     /translation="MKEERRRLLAKVAYLHYVQGKSQTLISKEMNIYRTTVCRMLAKA
                     KEEGIVRIEIADYDADLFALEEYVRKQYGLEKLDLVPNQVEDTPMDTLTNVAKTAAEV
                     FRHVVKDGDKIGLSWGATLSCLMDELNPKAMKDVFIYPLAGGPSHINAKYHVNTLVYR
                     LARIFHGNSAFMNAMVIQEDKHLAKGILQSKYFNDILTSWDQLDLALVGIGGEPNSLE
                     QSQWRDLLTSSDHDQLKYEKAVGEVCCRFFDQAGQPVYTGLQDRTIGISLEQLRRVPK
                     TMAVATGKHKAKAILAALKAGFINYLVTDKETMLAVLALDEDIDLNNVLL"
     gene            52637..52957
                     /locus_tag="VC122_000044"
     CDS             52637..52957
                     /locus_tag="VC122_000044"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009880818.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS lactose/cellobiose transporter subunit IIA"
                     /protein_id="HEP5640006.1"
                     /translation="MQVIVPDQIIMGLILNAGDAKQHIYQALKCAKEDDYATSEKEMA
                     LADDALLEAHNLQTQFLAQEASGNKSEITALFVHSQDHLMTTITEINLIKEIIDLRKE
                     LATK"
     gene            52985..53293
                     /locus_tag="VC122_000045"
     CDS             52985..53293
                     /locus_tag="VC122_000045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002991271.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HEP5640007.1"
                     /translation="MIKIGLFCAAGFSTGMLVNNMKVAAEKKGIDCQIEAYAQGKLAD
                     YAPLLDVALLGPQVAYTLDKSEAICKDNDIPIAVIPMADYGMLDGNKVLDLALSLVKE
                     "
     gene            53303..54607
                     /locus_tag="VC122_000046"
     CDS             53303..54607
                     /locus_tag="VC122_000046"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015057219.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIC"
                     /protein_id="HEP5640008.1"
                     /translation="MAKMNMQNIIKPIMTFVNMRGIIALKDGMLAILPLTVVGSLFLI
                     AGQIPFQGVNDAIASVFGADWTEPFMQVYHGTFAIMGLISCFAIGYSYAKNSDVEPLP
                     SGVLSLSAFFILLRSSYVPAEGEAIGDAISKVWFGGQGIIGAIVIGLTVGAVYTTFIR
                     RHIVIKMPDQVPQAIAKQFEAMIPAFVIFTLSMLVYIIAKSVTGGGTFIEMIYDVIQV
                     PLQGLTGSLYGALGIAFFISFLWWFGVHGQSVVNGIVTALLLSNLDANKALMAAGELS
                     LDKGAHIVTQQFLDSFLILSGSGITFGLVVAMIFAAKSKQYKALGKVAAFPALFNVNE
                     PVVFGFPIVMNPVMFLPFILVPVLAALTVYGAIAIGFMQPFAGVTLPWSTPAIISGFM
                     VGGWQGAIVQILILIMSTLVYFPFFKIQDNMAYQNEQASEES"
     gene            54818..57235
                     /locus_tag="VC122_000047"
     CDS             54818..57235
                     /locus_tag="VC122_000047"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003059593.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycyl radical protein"
                     /protein_id="HEP5640009.1"
                     /translation="MIETKSPYFGHLTDRMAHYREAVLDKKPYIDAERAILATEAYQK
                     HQNKPANLKRAYMLQTILENMTIYIEDESLIAGNQASSNKDAPIFPEYTLEFVLNELD
                     LFEKRDGDVFYITEETKQQLRDIAPFWENNNLRARCGVLLPEEVQVYMETGFFGMEGK
                     MNSGDAHLAVNYQKLLEHGLKGFEERARAAKAALDLTIPENIDKYHFYDSVFIVIDAV
                     KTYAKRYAKLARELAKTAKPERQAELLDIARICDKVPYEPAKTFAEAVQSVWFIQCIL
                     QIESNGHSLSYGRFDQYMYPYVKADLEAGRETEDTIVERLTNLWIKTLTINKVRSQAH
                     TFSSAGSPLYQNVTIGGQTRDKKDAVNPLSYLVLRSVAQTKLPQPNLTVRYHKGLDNT
                     FMNECIEVMKLGFGMPAMNNDEIIIPSFIKKGVSEEDAYDYSAIGCVETAVPGKWGYR
                     CTGMSYINFPKILLITMNDGIDPASGKRFAKGHGHFKDMTSYEELKAAWDATLREITR
                     MSVIVENAIDLGLEREVPDILCSALTDDCIGRGKTLKEGGAVYDYISGLQVGIANLSD
                     SLAALKKLVFEEGRLTPEELWHALESDFAGERGEEIRQMLINDAPKYGNDDDYADSLV
                     VEAYDTYIDEIAKYPNTRYGRGPIGGIRYSGTSSISANVGQGKGTLATPDGRHAGTPL
                     AEGCSPEHSMDKKGPTSVLKSVAKLPTDEIVGGVLLNQKVNPQTLAKEEDKLKLMALL
                     RTFFNRLHGYHIQYNVVSRETLIDAQKHPEKHRDLIVRVAGYSAFFNVLSKATQDDII
                     ERTEHTL"
     gene            57248..57916
                     /locus_tag="VC122_000048"
     CDS             57248..57916
                     /locus_tag="VC122_000048"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009880865.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fructose-6-phosphate aldolase"
                     /protein_id="HEP5640010.1"
                     /translation="MEYMLDTLDLEAIKKWHHILPLAGVTSNPSIAKKEGDIDFFERI
                     REVRAIIGDKASIHVQVIAQDYEGILKDAAEIRRQCGDSVYVKVPVTTEGLAAIKTLK
                     AEGYHITATAIYTTFQGLLAIEAGADYLAPYYNRMENLNIDPEAVIGQLAEAINRENA
                     DSKILAASFKNVAQVNKSFALGAQAITAGPDVFEAGFAMPSIQKAVDDFGKDWEAIHH
                     RKSI"
     gene            57973..59061
                     /locus_tag="VC122_000049"
     CDS             57973..59061
                     /locus_tag="VC122_000049"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002982378.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerol dehydrogenase"
                     /protein_id="HEP5640011.1"
                     /translation="MKVFASPSRYIQGKNALFTNAKTLKQLGNSPILLCDDVVYGIVG
                     ERFESYLIDNGMTPVHVAFNGEASDNEISRVVAIAKENGNDVIIGLGGGKTIDSAKAI
                     ADLLAVPVIIAPTIASTDAPTSALSVIYTDEGAFEKYIFYSKNPDLVLVDTQVICQAP
                     KRLLASGIADGLATWVEARAVMQKNGDTMAGGNQTLAGVAIAKACEQTLFADGLKAMA
                     SCDRQVVTPALENVIEANTLLSGLGFESAGLAAAHAIHNGFTALTGAIHHLTHGEKVA
                     YGTLTQLFLENRSREEIDRYIDFYQAIGMPTTLKEMHLDTATQEDFLKIGRQATMAGE
                     TIHQMPFVISPEDVAAALVAVDAYVTSR"
     gene            complement(59143..59652)
                     /locus_tag="VC122_000050"
     CDS             complement(59143..59652)
                     /locus_tag="VC122_000050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002992095.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatidylglycerophosphatase A"
                     /protein_id="HEP5640012.1"
                     /translation="MSTDHHLREVSYQLLAERGVSLEAIAELVLFLQNDYIPNLTMAE
                     CLESVEAVLAKREVQNAIITGVELDKLAEANQLSEPLLSILKTDQGLYGIDEILALSI
                     VNLYGSIGFTNYGYLDKTKPGIVDKLNHKDGNSCHTFLDDIVSAIAAAAASRIAHNDP
                     AKSAITNQQ"
     gene            60016..60831
                     /gene="sdaB"
                     /locus_tag="VC122_000051"
     CDS             60016..60831
                     /gene="sdaB"
                     /locus_tag="VC122_000051"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011055059.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="streptodornase B"
                     /protein_id="HEP5640013.1"
                     /translation="MNLLGSRRVFSKKCRLVKFSMVALVSATMAVTTVTLENTALARQ
                     TQVSNDVVLNDGASKYLNEALAWTFNDSPNYYKTLGTSQITPALFPKAGDILYSKLDE
                     LGRTRTARGTLTYANVEGSYGVRQSFGKNQNPAGWTGNPNHVKYKIEWLNGLSYVGDF
                     WNRSHLIADSLGGDALRVNAVTGTRTQNVGGRDQKGGMRYTEQRAQEWLEANRDGYLY
                     YEAAPIYNADELIPRAVVVSMQSSDNTINEKVLVYNTANGYTINYHNGTPTQK"
     gene            complement(61072..61914)
                     /locus_tag="VC122_000052"
     CDS             complement(61072..61914)
                     /locus_tag="VC122_000052"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002982409.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator"
                     /protein_id="HEP5640014.1"
                     /translation="MEIGETVEFIRHSKNISIKQVCGDYLTRQTYYRFIKNNLDISSK
                     KLLYILDNLNVNVDEFLFISNNFKQYKEFIDMDTAKHYFECRNIEGLNHILDSYKDSK
                     STKEKNLFALVKVLLATLTEEDCLTERTYLSNYLINIETWSHYETVLFNNCMFIFESC
                     FIEMVFSKVILNLDKYNTLRYYGNESIRMFVNMLILFIQRQEYDKASEILAKIEDYQL
                     NDDCLYERCCVSFFDGIIGLINGKEGAEQKCVQILEIFQLLNCKTIHHMFQTYLEAIK
                     HKLS"
     gene            62855..64051
                     /gene="speB"
                     /locus_tag="VC122_000053"
     CDS             62855..64051
                     /gene="speB"
                     /locus_tag="VC122_000053"
                     /EC_number="3.4.22.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010922720.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine proteinase exotoxin SpeB"
                     /protein_id="HEP5640015.1"
                     /translation="MNKKKLGIRLLSLLALGGFVLANPVFADQNFARNEKEAKDSAIT
                     FIQKSAAIKAGARSAEDIKLDKVNLGGELSGSNMYVYNISTGGFVIVSGDKRSPEILG
                     YSTSGSFDANGKENIASFMESYVEQIKENKKLDTTYAGTAEIKQPVVKSLLDSKGIHY
                     NQGNPYNLLTPVIEKVKPGEQSFVGQHAATGCVATATAQIMKYHNYPNKGLKDYTYTL
                     SSNNPYFNHPKNLFAAISTRQYNWNNILPTYSGRESNVQKMAISELMADVGISVDMDY
                     GPSSGSVGSSRVQRALKENFGYNQSVHQINRSDFSKQDWESQIDKELSQNQPVYYQGV
                     GKVGGHAFVIDGADGRNFYHVNWGWGGVSDGFFRLDALNPSALGTGGGAGGFNGYQSA
                     VVGIKP"
     gene            64053..64358
                     /locus_tag="VC122_000054"
     CDS             64053..64358
                     /locus_tag="VC122_000054"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002982424.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Spi family protease inhibitor"
                     /protein_id="HEP5640016.1"
                     /translation="MEMHFVRTEPEARRIAETFCAENTQTKTPMRVQQLSYPSDTDHS
                     GGELYIYALSPAGFIIVSGDTRAHTILGYSFDNNLDLNHDNVRSMIEAYQTQINSLD"
     gene            64836..65765
                     /gene="prsA"
                     /locus_tag="VC122_000055"
     CDS             64836..65765
                     /gene="prsA"
                     /locus_tag="VC122_000055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002991251.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase PrsA"
                     /protein_id="HEP5640017.1"
                     /translation="MKQMNKLITGVVTLATVVTLSACQSSHNNTKLVSMKGDTITVSD
                     FYNETKNTELAQKAMLSLVISRVFETQYANKVSDKEVEKAYKQTADQYGTSFKTVLAQ
                     SGLTPETYKKQIRLTKLVEYAVKEQAKNETISKKDYRQAYDAYTPTMTAEIMQFEKEE
                     DAKAALEAVKAEGADFAAIAKEKTIAADKKTTYTFDSGETTLPAEVVRAASGLKEGNR
                     SEIITALDPATSKRTYHIIKVTKKATKKADWKAYQKRLKDIIVTGKLKDPDFQNKVIA
                     KALDKANVKIKDKAFANILAQFAKPNQKQPAQK"
     gene            complement(66041..66277)
                     /locus_tag="VC122_000056"
     CDS             complement(66041..66277)
                     /locus_tag="VC122_000056"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002991249.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEP5640018.1"
                     /translation="MTHRFFLTPLSTLWRRLRPKRYSKKVLADEPTSYQPPAAHGPCD
                     DIHQLLEDKSLSDDYLIATSAMRHLWYWGYSQIG"
     gene            66701..69781
                     /locus_tag="VC122_000057"
     CDS             66701..69781
                     /locus_tag="VC122_000057"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014411903.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fibronectin binding protein"
                     /protein_id="HEP5640019.1"
                     /translation="MTNCKYKLRKLSVGLVSVGTMLIAPTVLGQEVSASSSTESSTTT
                     ANTGTGTASGMTATTPSATTDTGEAAGSGARSEANGASSVVSSEESQSSGTTPASPQA
                     QTAPAATSTSSASSSNEKTPKTATTTTSSTPVASTSNNSNKVTSTEAETQTMDVERYT
                     VDKENSKLNIKDGKTPKTRSSVNKDTKLIRNRDDKQRDIVDVTRTVKTNEDGTIDVTV
                     TVKPKQIDEGADVMALLDVSQKMTKENFDKAKEQIKKMVTTLTGEPTDGKENHNRRNS
                     VRLMTFYRKVNEPIELTAENVDKTLDEVWKKAKEDWDWGVDLQGAIHKAREIFNKEKE
                     KKSGKRQHIVLFSQGESTFSYDIKNKSDITKTKITEKVTTSSPLFPWLPIFNHTNRKA
                     EIIGDLEKVLDMAEKVGISLPSSLKLAVKALGLTNSAIGSILGKGLTEYLGLTEYSSD
                     NLDGGGFDYSKRVGEGYYYHSFSDRKYENTMPLEEAIRTALASNFPKLTDNWFFDILN
                     SFVNKDTVEKAKLDVIMKVLNSIFYKREYRYYNHNLSAIAEAKMAQQEGITFYSVDVT
                     DLNSASKRVRRQAAVRKGTKEEENKKNEEERNTKFDTYLKKMSEGNNFLSNVEERDKF
                     KDTLTELTIKDEFTDKVTVEKDSWSKSITDGLKNSNNNNVKHQQANTSTWSFFSSSKE
                     SLTWIISKEQLKEAFEKNGSLTFKYKLRVNKDKLLDKDKKRTKRDTPTENKTSVTADI
                     ISNTVDYKINNQEVKGNKLDAVKLTYTKETVPVPDVEGEVVPIPEKPLVEPMTPLYPA
                     IPNYPTPQLPKDEDLEISGGHGPIVDIVEDTGTGVEGGAQNGVVSTQENKDPIVDITE
                     DTQPGMSGSNDATVVEEDTAPKRPDVLVGGQSDPIDITEDTQPSVSGSNDATVVEEDT
                     VPKRPDILVGGQSDPIDITEDTQPGMSGSNDATVIEEDTKPKRFFHFDNEPQAPEKPK
                     EQPSLSLPQAPVYKAAHHLPASGDKREASFTIVALTIIGAAGLLSKKRRDTEEN"
     gene            69961..71904
                     /locus_tag="VC122_000058"
     CDS             69961..71904
                     /locus_tag="VC122_000058"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002991243.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fibronectin binding protein"
                     /protein_id="HEP5640020.1"
                     /translation="MTKKFIKSAVILGLASITISMTQPVEAISNSLEYQAQYERVKEE
                     LDALKEKGPKLGERSLYFRLVNKFLSWFGQVWDPLAIKETDYLLDLIGQVNYLVSNTE
                     FQAPEYKDAKNKLDLAVAGYDLYLAREKKRGDAPDYQVTQLTLEQLKTIKKKIREALE
                     TFAQEVKTIQQKNPDLFPHAYVLNAKSKDITDKAQPFLVNSYYDVFYKQFLFTNTNDG
                     YNYNKEADNLTSYQNLIKELLGLKAEIDQLTKGKTGDDRLEMLMWQSKFTLSDSRKKS
                     HLSAYYTQGEYVDKVVLSEEAPNLRTAKEKVKTLKGILHDYYVETNKPEKAKKYHVED
                     ETLPSQPEAPATPEAPAPVPAPVPVPGQKPAEDERSPQATEPANPSKEDSSTDASQGS
                     HDSENPATDSPSQPQAPDQGNHQSQVPNAKPQTDKTDTPNAPAIPQDTPKAPEAGGQS
                     GPAGNTEEKAPDSGPKESDQSSSKESPSVGEDTTPNQPDVLVGGQSEPIDITEDTITD
                     APPTVSGHNASTQPQSVVEDTAPQRPDVLVGGQSEPIDITQDTQPGMSGSNDATVINE
                     DTKPKRVFHFDNKEPQASEKAAEQKLAPHDSHTTPQASDDTAAPHLPASGDKPEKVFA
                     LAALTMVAAAGLLSKKRRNTDKN"
     gene            72046..72342
                     /locus_tag="VC122_000059"
     CDS             72046..72342
                     /locus_tag="VC122_000059"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002982439.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4298 domain-containing protein"
                     /protein_id="HEP5640021.1"
                     /translation="MTKQDQLIVEKMEQTYEAFSPKLANLIEALDAFKEHYEEYATLR
                     NFYSSDEWFRLANQPWDDIPCGVLSEDLLFDMIGDHNQLLADVLDLAPIMYKHM"
     gene            72491..72775
                     /locus_tag="VC122_000060"
     CDS             72491..72775
                     /locus_tag="VC122_000060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002982442.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter"
                     /protein_id="HEP5640022.1"
                     /translation="MKFKKVLVIPALALAATCFLTACGTKKDSKKEEVKEIKMSDIKD
                     DAVSKKTKVVDGEEVTEYTTKDGNVIQIPAGNEEGMESKDAGGSGAPAKN"
     gene            73209..74477
                     /locus_tag="VC122_000061"
     CDS             73209..74477
                     /locus_tag="VC122_000061"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002982445.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter periplasmic adaptor
                     subunit"
                     /protein_id="HEP5640023.1"
                     /translation="MFQLRKKMTRKQLALLSAGVLTCVVGGSYLIMNHQQQEIVSSVN
                     KVKALTIKEAMEQGKDISLTLAGEVTANNSSKVKIDSSKGEVKDVFVKKGDVVKVGQP
                     LFSYETSQRLTAQSSEFDVQTKANQLQVAKTNAALKWETYNRKVNEINTLKSRYNTAP
                     DESLLEQIRSAEDSVSQALSDAKTADSDVKTAQIELDKANATATTEKGKLEYDTVKSD
                     TAGTIVSLNTDLPNQSKSKKENETFMEIIDKSKMLVKGNISEFDRDKLKIGQKVEVID
                     RKDNSKKWTGKVTQVGNLKAEEKGQGQGQGGNDQQDNPNQAKFPYVIELDQSDKQPLI
                     GSHTYVNVLNNVPEAGKIVLKETFTMAENGKTYVWKVDKNKVKKQEIKTKPFSKGYVE
                     VTSGLTMQDKIAQPLPGMKDGMEVGSIVKP"
     gene            74464..75138
                     /locus_tag="VC122_000062"
     CDS             74464..75138
                     /locus_tag="VC122_000062"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002982458.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEP5640024.1"
                     /translation="MLNLKDIRKSYHLGTEEFAILKGIDLEVNEGDFLAIMGPSGSGK
                     STLMNIIGCLDKPGSGSYAIEGRDVSSLSDNELADLRNQKIGFVFQNFNLMPKLTACQ
                     NVELPLTYMNVPKKERRKRALEMLKLVGLEERSEFKPMELSGGQKQRVAIARALVTNP
                     SFILGDEPTGALDTKTSVQIMDLFKQFNDNGKTIIIITHEPEVAALCKKTVILRDGNI
                     EHSDIE"
     gene            75151..76368
                     /locus_tag="VC122_000063"
     CDS             75151..76368
                     /locus_tag="VC122_000063"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002993552.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEP5640025.1"
                     /translation="MEDIIAALSSILSHKMRSILTMLGIIIGIGAIIAIFSIIEGNTE
                     NTKRQLIGGSNNTINIVFNKKSSIDPKFPDKSNAKKPDYLPFMAEEELSKIQQVKGVK
                     NALISYGIDDKVYHLGQKSSAKISAITKNVAEVRRMTFIKGSDFSDKDFIDQKQVIYL
                     EKSLYESLFPKDDGLGKFVEVMGNPFRVIGVFESKEQSGLTSGTEKIAYIPLHQWYNI
                     NGVVDATPEITIQTYRADDLKPVAKRVSDMLNQTIPKSDYMFGVMNLKEFERQLDNLN
                     KSNFVLLAGIASISLIVGGIGVMNIMLVSVTERTREIGIKKALGARRKLILKQFLIEA
                     VILTLLGGVIGVISGMVSGLIITRSLEYPYILSLFSVVLSLAFCCIIGIVFGLLPAIK
                     ASKLDPIEALRFE"
     gene            76462..77115
                     /locus_tag="VC122_000064"
     CDS             76462..77115
                     /locus_tag="VC122_000064"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003059317.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HEP5640026.1"
                     /translation="MFKILVVEDDDTISQVICEFLKANNYDPDCVFDGQAALDKWQTT
                     SYDLIILDIMLPSLSGLEVLKTIRKTSDVPIIMLTALDDEYTQLVSFNHLISDYVTKP
                     FSPLILIKRIENVLRVSTPDEKRQIGDLLVDETEHSVYWQGTLVKLTKKEYDIIDYLA
                     KRHQKIVTRDQLMDDIWGYSELDTRVLDNHIKNLRKKMTGIPLKTITGMGYLLGERE"
     gene            77112..78500
                     /locus_tag="VC122_000065"
     CDS             77112..78500
                     /locus_tag="VC122_000065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011285225.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing histidine kinase"
                     /protein_id="HEP5640027.1"
                     /translation="MRLIKKTFLVINGLIIVVVTSILLVLYFAMPIYYTKVKDKEVKR
                     EFDQTSKQIKGKTVTEIRDILTKKINKDNIWYSLVDSDNQLLYPSLQLLDGVSESKDS
                     QNVNIVTTFDNSYSNVKVMSQKVTLRDGKKMTLLGQSSLQPVTDASKVLLDLYPSLLM
                     FSVTVGSIVAYLYSRTSSRRILSMSQTAKKMVNLEPNLTCTIHGKDEIAMLASDINRL
                     YASLSTSIKSLQKEYEKASDSEREKSEFLRMTSHELKTPITSVIGMIDGMLYNVGDFA
                     DRDKYLRKCRDVLEGQAQLVQSILSLSKIETLASQNQELFSLKSSLEEEMEVFLVLSE
                     LKHLKVTINLEEQFVKANKVYLLKAIKNIIDNAFHYTKSGGQVMIQLKDNQLVIKNEA
                     ETLLTQQQMKQLFQPFYRPDYSRNRKDGGTGLGLFITHQILDQHHLAYRFVVLDQRWM
                     VFTIDFPSHHDD"
     gene            78602..80227
                     /locus_tag="VC122_000066"
     CDS             78602..80227
                     /locus_tag="VC122_000066"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002991233.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CHAP domain-containing protein"
                     /protein_id="HEP5640028.1"
                     /translation="MKKRKLLAVTLLSTILLNSAVPLVVADTSLRNNTSSTDQPTTAD
                     TDTDDESETAKKDKKSKETASQHDTQKDHKSSHTHLTPPSNDTKQTDQASSEATDKPN
                     KDKNDTKQPDSSDQSTPSPKDQSSQKESQNKDGRPTPSPDQQKDQTPDKTPEKSADKT
                     PEKGPEKATDKTPEPNRDAPKPIQPPLAAAPVFAPWRESDKDLSKLKPSSRSSAAYVR
                     HWTGDSAYTHNLLSRRYGITAEQLDGFLNSLGIHYDKERLNGKRLLEWEKLTGLDVRA
                     IVAIAMAESSLGTQGVAKEKGANMFGYGAFDFNPNNAKKYSDEVAIRHMVEDTIIANK
                     NQTFERQDLKAKKWSLGQLDTLIDGGVYFTDTSGSGQRRADIMIKLDQWIDDHGSTPE
                     IPEHLKITSGTQFSEVPVGYKRSQPQNVLTYKSETYSFGQCTWYAYNRVKELGYQVDR
                     YMGNGGDWQRKPGFVTTHKPKVGYVVSFAPGQAGADATYGHVAVVEQIKEDGSILISE
                     SNVMGLGTISYRTFTAEQARLLTYVVGDKLPRP"
     gene            80305..80556
                     /locus_tag="VC122_000067"
     CDS             80305..80556
                     /locus_tag="VC122_000067"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002982472.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEP5640029.1"
                     /translation="MIKKVTTPSQKIKKRVRNGYLLKLGTACLLLSILSYGIGLLGQP
                     SMENTFMGIASVAMLGSVCFFIIFALNRIFDALEDNLRD"
     gene            81232..82839
                     /locus_tag="VC122_000068"
     CDS             81232..82839
                     /locus_tag="VC122_000068"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009880571.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HEP5640030.1"
                     /translation="MHVSKLFTSQQWRELKLISYLTENSNAIGVKDKELSKALNISML
                     TLQSCLTNMQFMKEVGGITYKDGYINIWYHQCCGLQEVYQKALRESPSLKLLELLFFR
                     DFSSLEELAEELFVSLSTLKRLIKKTNTYLSHTFAISIVTSPVQVSGDERQIRLFYLK
                     YFSEAYKISEWPFGDILNLKNCERLLSLLIKEVDVKVHFTLFQHLKILSGVNLIRYYK
                     GYSCSYNNKKTSHRFSQLIQHSSEIQDLSRLFYLKFGLHLDEYTIAEMFSNHLNDKLE
                     IGCAFEIINQDPISGGRQVTNWIHLLDEMEIKLNLSITNKYEVAVTLHNASVLNEEDI
                     TANYLLFDYKKSYLNFYQKEHPRIYEAFVTSVEKLMQADNAQVSKELINQLTYCFFIT
                     WENSFLKVNQKEKVRLLVIERSYNSVGNFLKKYIGEFFSITNFDELDCLTIDLVEIEK
                     QYDVIVTDVMVGKSEELEIFFFYKMIPEAIIDRLNEFLNVSFTDNNVMVKNLEAPSPS
                     KSHSDKEVQKPEKPDNSVKQATSATSS"
     gene            83029..84078
                     /locus_tag="VC122_000069"
     CDS             83029..84078
                     /locus_tag="VC122_000069"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014407887.1"
                     /note="The YSIRK form of extended signal peptide directs
                     nascent proteins to the cross-wall site, while signal
                     peptides lacking YSIRK direct proteins instead to the cell
                     pole. A large fraction of YSIRK proteins are surface
                     proteins anchored by sortase-mediated processing of a
                     C-terminal LPXTG motif; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YSIRK-type signal peptide-containing protein"
                     /protein_id="HEP5640031.1"
                     /translation="MSKRNPNKHYSLRKLKTGTASVAVALTVLGTGLANTTDVKAETE
                     HVDVVLSAKEANKVFEERKALEKQARDLGDTINHMSQTISEQSRKIAALKSEAELKNQ
                     QALEALNNKNEQIAKLTNENAQLKEAIEGYVQTIQNASREIAAKQQELAAAKSQLEAK
                     NAEIEALKQQDASKTEEIAKLQSEAATLENLLGSAKRELTDLQAKLDTANTEKEKLQS
                     QAAALEKQLEATKKELADLQAKLAATNQEKEKLEAEAKALKEQLAKQAEELAKLKADK
                     ASGAQKPDTKPGNKEVPTRPSQTRTNTNKAPMAQTKRQLPSTGEETTNPFFTAAAATV
                     IASAGVLALKRKEEN"
     gene            84303..85379
                     /locus_tag="VC122_000070"
     CDS             84303..85379
                     /locus_tag="VC122_000070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014407885.1"
                     /note="The YSIRK form of extended signal peptide directs
                     nascent proteins to the cross-wall site, while signal
                     peptides lacking YSIRK direct proteins instead to the cell
                     pole. A large fraction of YSIRK proteins are surface
                     proteins anchored by sortase-mediated processing of a
                     C-terminal LPXTG motif; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YSIRK-type signal peptide-containing protein"
                     /protein_id="HEP5640032.1"
                     /translation="MARKDTNKQYSLRKLKTGTASVAVAVAVLGAGFANQTEVKAAES
                     PKSTETSANGADKLADAYNTLLTEHEKLRDEYYTLIDAKEEEPRYKALRGENQDLREK
                     EGKYQDKIKKLEEKEKNLEKKSEDVERHYLKKLDQEHKEQEERQKNLEELERQSQREI
                     DKRYQEQLQKQQQLETEKQISEASRKSLSRDLEASRAAKKDLEAEHQKLKEEKQISDA
                     SRQGLSRDLEASREAKKKVEADLAEANSKLQALEKLNKELEEGKKLSEKEKAELQARL
                     EAEAKALKEQLAKQAEELAKLKGNQTPNAKVAPQANRSRSAMTQQKRTLPSTGEAANP
                     FFTAAAATVMVSAGMLALKRKEEN"
     gene            85590..86522
                     /locus_tag="VC122_000071"
                     /pseudo
     CDS             85590..86522
                     /locus_tag="VC122_000071"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012561044.1"
                     /note="The YSIRK form of extended signal peptide directs
                     nascent proteins to the cross-wall site, while signal
                     peptides lacking YSIRK direct proteins instead to the cell
                     pole. A large fraction of YSIRK proteins are surface
                     proteins anchored by sortase-mediated processing of a
                     C-terminal LPXTG motif.; internal stop; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="YSIRK-type signal peptide-containing protein"
     gene            86857..90405
                     /gene="scpA/B"
                     /locus_tag="VC122_000072"
     CDS             86857..90405
                     /gene="scpA/B"
                     /locus_tag="VC122_000072"
                     /EC_number="3.4.21.110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_021340616.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="C5a peptidase ScpA/B"
                     /protein_id="HEP5640033.1"
                     /translation="MRKKQKLPFDKLAIALMSTSILLNAQSDIKANTVTEDTPATEQA
                     VEPPQPTAVSEEAPSSSKETNPPQIPDDAEETVADKANDPAPQAPAKTADTPATSKAT
                     IRDLNDPSQVKTLQEKAGKGAGTVVAVIDAGFDKNHEAWRLTDKTKARYQSKEDLEKA
                     KKEHGITYGEWVNDKVAYYHDYSKDGKTAVDQEHGTHVSGILSGNAPSETKEPYRLEG
                     AMPEAQLLLMRVEIVNGLADYARNYAQAIRDAVNLGAKVINMSFGNAALAYANLPDET
                     KKAFDYAKSKGVSIVTSAGNDSSFGGKTRLPLADHPDYGVVGTPAAADSTLTVASYSP
                     DKQLTETATVKTDDHQDKEMPVLSTNRFEPNKAYDYAYANRGTKEDDFKDVEGKIALI
                     ERGDIDFKDKIANAKKAGAVGVLIYDNQDKGFPIELPNVDQMPAAFISRKDGLLLKDN
                     PQKTITFNATPKVLPTASGTKLSRFSSWGLTADGNIKPDIAAPGQDILSSVANNKYAK
                     LSGTSMSAPLVAGIMGLLQKQYETQYPDMTPSERLDLAKKVLMSSATALYDEDEKAYF
                     SPRQQGAGAVDAKKASAATMYVTDKDNTSSKVHLNNVSDKFEVTVTVHNKSDKPQELY
                     YQATVQTDKVDGKHFALAPKALYETSWQKITIPANSSKQVTVPIDASRFSKDLLAQMK
                     NGYFLEGFVRFKQDPKKEELMSIPYIGFRGDFGNLSALEKPIYDSKDGSSYYHEANSD
                     AKDQLDGDGLQFYALKNNFTALTTESNPWTIIKAVKEGVENIEDIESSEITETIFAGT
                     FAKQDDDSHYYIHRHANGKPYAAISPNGDGNRDYVQFQGTFLRNAKNLVAEVLDKEGN
                     VVWTSEVTEQVVKNYNNDLASTLGSTRFEKTRWDGKDKDGKVVANGTYTYRVRYTPIS
                     SGAKEQHTDFDVIVDNTTPEVATSATFSTEDRRLTLASKPKTSQPIYRERIAYTYMDE
                     DLPTTEYISPNEDGTFTLPEEAETMEGGTVPLKMSDFTYVVEDMAGNITYTPVTKLLE
                     GHSNKPEQDGSDQAPDKKPETKPEQDGSGQAPDKKPEAKPEQDGSGQAPDKKPEAKPE
                     QDGSGQTPNKKPETKPEKDSSGQTPGKTPQKGQPSRALEKRSSKRALATKASTRDQLP
                     TTNDKDTNRLHLLKLVMTTFFLGLVAHIFKTKRTED"
     gene            90502..91758
                     /locus_tag="VC122_000073"
     CDS             90502..91758
                     /locus_tag="VC122_000073"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011285739.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YSIRK signal domain/LPXTG anchor domain surface
                     protein"
                     /protein_id="HEP5640034.1"
                     /translation="MRRAENNKHSRYSIRKLSVGVTSIAIASLFLGKVAYAVDSMQPI
                     SSTQGNRANTSKKWYDINEYGLIPVDISFEAAKKDFKEEVEKSLLSEEQQKTYNQKIE
                     AARNKDDLLLTYHSEYSKDIKKHSTSTEPVEASVQETQASVSDSMVTDDSTSVTADSP
                     EKTPSTETPAPSIPEVPEAPASSPESEESSVASSEETSSPETPVAPEAPALSEAPAQP
                     AESEESSVAAPSEETPSPETPAAPETPEEPAAPSPSPESEEPSVAASSEETPSPETPA
                     APETPEEPAAPAQPAESEESSVAATTSPSPSTPAESETQTPPAVTKDSDKPSSAAEKP
                     AASSLVSEQTVQQPTSKRSSDKKEEQEQSYSPNRSLSRQVRAHESGKYLPSTGEKAQP
                     LFIATMTLMSLFGSLLVTKRQKETKK"
BASE COUNT        29333 a        17923 c        19349 g        25470 t
ORIGIN      
        1 aaaagtttta ttgtttatca gaaaaagtaa tctaatatca atatattgtg ttcgagcaaa
       61 aaacttgaca caatatattg atttttttag ttgtcgtgat ataatagttc cgtaaggaga
      121 tttgatatgg taagtttaga agaagacaag gtgactgttc aacctgatat taaagtgatt
      181 aaacgagatg gtcgccttgt taattttgat agtacaaaaa tctatagtgc tttattaaaa
      241 gcaagcatga aagtaactcg gatgtcgcca cttgttgagg ctaaattaga ggctatttct
      301 gatcgcatta tagcagaaat tattgagcgt tttccaacta atatcaaaat ttatgaaatc
      361 caaaatattg tagagcataa gcttcttgca gctaatgaat atgctattgc aaaagaatac
      421 attaattatc gtactcagcg tgactttgca cgttcacaag caacagatat caatttttct
      481 attgataaat taattaataa agatcaaaca gttgttaatg aaaatgctaa caaagatagc
      541 gatgttttta atactcaacg agatttaact gctggaatcg tagggaaatc gattggttta
      601 aaaatgttac cttcgcatgt tgctaatgct catcaaaaag gagatatcca ttaccatgat
      661 ttggattaca gtccttatac accgatgacg aactgctgtt taattgactt taagggcatg
      721 ttagccaatg gctttaaaat tggtaatgct gaagtggaaa gtcccaagtc tattcaaact
      781 gcaacagctc agatctcaca gattattgcg aatgtagcat caagtcagta cggcggatgc
      841 acagctgatc gcattgacga gtttttagcc ccatatgcgg agcttaactt taaaaaacat
      901 atggctgatg ctaagaaatg gatcgttgag actaagagag aaagctatgc ttttgaaaag
      961 actcaaaaag atatttatga tgcgatgcag tctttggagt atgaaattaa tacgctcttt
     1021 acgtctaatg gtcaaacacc atttacttct ttaggatttg gtttggggac gtcttggttt
     1081 gaacgtgaga ttcaaaaagc tattttgacc attcggatta atggtcttgg tagtgaacat
     1141 cgcacggcta ttttccctaa attaattttc acggttaaac gtggcttgaa tttagaacca
     1201 gattcaccaa actatgatat taagactttg gctttagaat gtgcgactaa gcggatgtac
     1261 ccggatatgt tatcttatga taaaattatt gatttgacag gatctttcaa atctccaatg
     1321 ggatgccgct ctttccttca aggctggaaa gatgaaaatg ggcaagatgt gacctcaggc
     1381 cgtatgaatc ttggggttgt caccctcaat ttacctcgca ttgccatgga atcaaatggc
     1441 gatatggata agttttggga gctgtttaat gagaggatgc taattagtaa ggatgcttta
     1501 atttatcgtg tcgaacgtgt cacagaagca aaaccagcaa atgcccctat tctttatcaa
     1561 tatggtgctt ttggaaagcg tttggagaag acagggaatg taaatgatct ctttaagaat
     1621 cgtcgtgcaa cagtctctct tggctatatt ggtctttatg aagtggcgtc tgttttttat
     1681 ggtggtcaat gggaaggtaa tccagatgct aaagctttta ccttgtcaat tatcaaggca
     1741 atgaaacagg cctgtgagga ttggtcagat gaatatggtt atcatttctc tgtttattcg
     1801 actccatcag aaagtttgac agatcgcttt tgtcgtttag atacggaaaa atttggcatt
     1861 gtgacagata ttacggataa agaatactat acaaattctt ttcactatga tgtgcgtaag
     1921 agtccgacac cttttgaaaa attagatttt gaaaaagatt atccagaagc aggtgcttca
     1981 ggtggtttta tccactactg tgagtatcct gttttgcaac aaaatccaaa ggccttggaa
     2041 gcggtttggg actatgctta tgatcgtgtg gggtatttgg gaaccaatac gcctattgat
     2101 aaatgctata attgccaatt tgaaggcgat tttaccccaa cagaacgtgg ttttacttgc
     2161 ccaaactgtg gcaataatga ccctaaaaca gttgatgtgg tcaaacgtac atgtggttac
     2221 ttggggaatc ctcaggcccg cccaatggtt aacggtcgcc ataaggaaat ctctgcgcgt
     2281 gtaaaacata tgaatggttc tactataaaa tacccaggcc tgtaaactca agctggggga
     2341 gagccctttt gatggaaata tgatatgctt aaaaggttgc ttcatgcaag caatctttta
     2401 ttgtttaatg gtttcttagc aattgtgaga taagaataac taatccaaaa cgactaatta
     2461 gctgttagca atcggactat aagaagaaag agataaggaa gaaataatgg gaaaatatca
     2521 attagactat aaaggaatgc agcaggtaga acgctttcat gagaagcatt ctaaaaaaaa
     2581 gacggataaa aaatcccgcg ttcaagaact taaggctcgg tttttagaga agtcaaaaaa
     2641 acaggataac taaagtatcc aataaagaaa gaaggttgag tatgcttaat attgggattg
     2701 tgggactagg tgccatttca caaaaagcct atttaccata tatgagacag ctgagtgaca
     2761 ttacttggca tctatcaacg cggaatgcag cagtacgtca gcaggtcggt cagttatttg
     2821 gtcacgctat tctttacagt gatgtcaagg agttgtcaaa ggcaagttta gatggcgtct
     2881 ttattcatgc ggccacatcg gcccacgcag agttggctag tttattttta aatcagggga
     2941 tcccagtctt tatggataaa cctatagctg acaattacct gatgacaaag aacctctatg
     3001 acttagccaa agaaaatcag acttttctga tggcaggatt taacagacgt tttacacctc
     3061 gtgtcaagaa gttgtcaagt ttgtcaacta agcgtaaagt ggctgttgaa aaaaatgact
     3121 tgaaccggcc aggagatatg acttttaagc tctttgattt ctttattcat cctttagata
     3181 cagccttatt cttgtcagaa gggaccttac taaaagggca ttttcaatat catctggaag
     3241 caggcttact gagtcaagtt atggtaacct tgatgaccga aagcatgacg actacagctt
     3301 ctatgaattt acaatcaggg agtcgccgtg aggtaatgga agttcaacgg gctgaagaaa
     3361 cttatcattt agagaactta gacgagttat ctatttataa aggtactgaa aaaagggtgc
     3421 ttggctttgc ttcttgggac actaccttgc acaaaagagg ttttgaaaca atgatcgatg
     3481 cctttttaga agctattagc acaggcgtca atcctgtcag tcctgagtct agtcttttaa
     3541 gccactggat ttgtcaacag attgctgact ctcagctttc ttatggagag ttaacggtag
     3601 agttgcctaa ggattaggaa ttgatatgga aataagacga ccgactttaa aagataaaga
     3661 cgctgtattg tcaatgatta acgaattttt agagcagaaa agtgctacag atgggttatg
     3721 gcattttaac gtcaatgact ttaactatga aacatggttg gaagattcct tgcgacagga
     3781 aatgggtttg tccagtcaag gagtgcctgc tattcaatac gtcgcatttg atgagagaag
     3841 tcaggctatc gggttcttaa atcttcgttt acggttaaat gagagattac ttgaaaaagg
     3901 tgggcatatt ggttactctg tccgtcccag ccagcgtggg aaaggttatg ccaaggagat
     3961 gcttaaacaa gctgtcagct atgcgatttc caagaatatc acgacaattt tggtaacttg
     4021 tgatgagact aatgttgcaa gccgagcagt tattgtggct aacggtggtg tcttggaaga
     4081 tagtcgaggt ggaacggaac gttattggat tgaagaacaa agaggcgata agtgatggca
     4141 gaaaagtgtt ggaataatcc taagccaaag gaatggcagg cagaggagtt aagtcaaggc
     4201 cggattattg actataaggc ttttaacttt gttgatggtg aaggagttcg taattccctt
     4261 tatgtgtctg gttgtttatt ccattgcaag gggtgttata atgctgcgac ttggtctttt
     4321 aaggcgggaa tgccttatac gcaagaactt gaagagcaga taatgacgga tttggcacag
     4381 ccttatgttc agggactgac acttttaggt ggagaaccct ttttgaatac gggtatctta
     4441 attcctctga ttaagcgtat tcggcgtgaa ttgccagaaa aagatatttg gtcttggacg
     4501 ggttatactt gggaagaaat gatgcttgaa acaccagaca aactggaaat gctgtcctta
     4561 attgatattc tggtagatgg tcgttttgat atcacaaaaa agaacctcat gttgcaattt
     4621 agaggctctt ctaaccagcg aattatcgat gttcaaaagt ctttggctgc taaagaagtg
     4681 attatctggg ataagttaaa cgatggggac caaacctttg aacaaattag tcgggaggat
     4741 ttgctttaag aagctgactt atccacatga tttttttgaa aaggtcatgt gaaaacaggt
     4801 cctttaaggg tgaattgggg agaaagtaat tgttatttcc tggttttccc cacattatcc
     4861 tcatagttat ccacaggttg tgtataagtt gtagaattgt gaataaagac taaaaaagag
     4921 gcctaggcct cttttttgat atagacaaat tcatgatctg ttgataggtc tggatggtag
     4981 taaaaaccag caaagacaag gcttttaagg ctgtcaacag tttggcaatt attttccata
     5041 caggcagtga gaaatgcacc tcgtgccttt tttgaaatgg tggaatgggt tttgaattgg
     5101 ccctcttttt ctgccataaa cttgggactt atccacaatt gtttacagtc tttggaaaag
     5161 acatcgtcaa attcactgga taaaagggaa atcacttggg gatgctcttt ggcaaactga
     5221 ttataacagg gccgccagta agacttgagg ctttgtcctt caatttttat tctggtgtga
     5281 aaatcatggc ggtgctctgc gataggatga ttagcgggaa tgataccgta aaaggatgag
     5341 gtgatgtaaa cttgctgggt gaggtaagcc tgttcttggg tagtgagctt atcgcgcttg
     5401 atatggcggt acataaggcc attgaacagt tggtaggcag ggtaagccaa gttttgttga
     5461 gaggccatgt cttgccagcg ttgctgttct tttttagctg cctcttcttt gatacgatag
     5521 gatttggcca agtcctccgt ggtcatagct gccatgattt ttaaaatggc ttggctgtct
     5581 tgtggaagga ggtgtgggtg agattcctta gggatcatca tttcttttgc ggttggaatt
     5641 aaaaaggtta gcatagtgtt attgtagtaa agttacttgc tttctgtcaa gaaaactagt
     5701 cttgtccaag gatgacgttc acacagatac cttcctcgtc ttctgtcgtg aaagaagaag
     5761 aatcttcttg taaaatagct aggccgtgta atcttgcttt tttcagagta gctgaaaaaa
     5821 gcaggggcgt ctcaatgtga atggtcaaga aggctaagcc tggggcacct tcttggtgtt
     5881 ttttgaggta aggggctgac cagtgattga atgcaaggtg atgatggtag ttccctgaag
     5941 caatccagct agcacttgga atggtcattt tatcgcctaa atcaaaaacc ttttgataga
     6001 gaagtgaaga agccagagca tttttgacac tcagatggac atggccaatg cgtgtgtctt
     6061 gagctagtaa aaagcgcttg gggatatctg tcaactgctc caatatgctt ttggtatccg
     6121 tgggctcagt cacaccaata atttgaccat tgtcgcggat atcccaatgc tcaacagctt
     6181 tgtcatgata aatttcaatc ccattgccct caggaccgct gaggtagata gcttcgctat
     6241 agccgtgatc agctgatcct tctaaagaga tacctctggt taagaaatga ttgagaacaa
     6301 gtcccaagct atgacgatca ggaactaaaa aggatgtgtg ataaagacca taagccttat
     6361 ttgctggtaa aggtgtttgt cgtaactcta agataacggt tttaccgtct gtagtcaatt
     6421 gacgagatgt cgtgtcttgt gagagaacct gtaaaccgat aatacttgtg taaaaggttg
     6481 tcatctttgc tagatcggta acatttagag aaaccgttcc taaagagatg gttgagttgt
     6541 aagggtatgt catcctatta cctccaaaaa ctaattaaat ctatcataat caatcagttt
     6601 caaaaagaaa agtaggcaca aataagtggt ataggcacta aaaagtaact catttgtcag
     6661 ccctatcttg tttctgttct ctctaatagt ctataatggg ttataagtta caaaaagata
     6721 ctaagaggag ctgctgccta tgctgaaaaa aagaccggct tgtcccgtag aaacaacctt
     6781 atcggtaatc gggaataaat agaaattatt aatattaagg gacttgctaa agggaccttg
     6841 cgttttgggc agttgaagtc ctcaataggt ttggtcagtc aaaaggtact tactgcacaa
     6901 ttaagggcta tggaagcaga cggattggtt catcgggaag tgtatgcaga agtgccacct
     6961 cgggtggagt attccttaac agaaactggg ttgagcttag ctcctgttat tgaagcgatg
     7021 tcggattggg gccaaactta ccaagaaaaa catgccgatt tatttgaaga aggctagagg
     7081 aaagagttgt ccgagcatac tgagacttag agaagctgat agtttttaaa aaatgatatt
     7141 atcaaattct ttacagtttg ataaaattac tttttttcta caaaaagact tgcgctcatc
     7201 tcacgaatat gataatataa tatcaagtat gtttgagaaa acagaagctc aggtcaagta
     7261 agtgcacact tacaataaca cagttgataa tggggaaact cagagtcaag aaaaagaaaa
     7321 gcaagccttg cattttgcaa ggcttttttc tgtctttatt ttggtctgcg ccgagcaaaa
     7381 tctaaaaatt taaatttttc cagtttatgg cgactctcgg tgaattggaa ttggttatta
     7441 ttggaaaggt agactttgga tttgacggaa acaacatgtt ggtctgatcc aaggtcaagt
     7501 aaaattttat cacgatcggt tatttggtca atggtaacct ctttgagggc aaagtcaatg
     7561 gcgagatgga gttccttttc caaatattga taaatagaat gctctgcgat ttcccttgtc
     7621 attattggca ccaaagtttt actaaggtaa tcaatgtcta agacggaagc cacaccctca
     7681 acaacgcgtt ggcgtgtaac acgccagacc aagctgtttt tgctaaaacc ggtcaatttt
     7741 gaaagtgtct catccacaat cagtttatca atggcaatga cgttggtctt agaatctaga
     7801 tttgagtaag agacgagttc ctgataacta gttagttctg agatagggaa catgatctga
     7861 tgatgtttaa tcacttgggt gccacgtcct tgttttttga ggataagacc tgcctttgtg
     7921 agaagtgaga gagccttgcg gacggtatct cggctagctt ggtattgttg actcaattct
     7981 atttcagtag gtaaaaaatc cccttctttg taaaagtctt tgtttatttt tgtttcgagg
     8041 tctttataaa tacgttcata ctttgtcata gggtaaatta cctttccatt tgtctatttt
     8101 tttacagaca acacctttta ttttaccaaa ataatggctt ttttggcaca aacaacttgt
     8161 agacaaattt gcaatcgatt acaataaagg tgtcagttag attctatcta agaatttgaa
     8221 tatttcattt tgacgttgct tgttaaaagc aactagaaca aaggactagg ggatgtcaca
     8281 tgggaaaatt tgaacaggat gctaagagtc ttctaactgc tattggcggt aaagaaaaca
     8341 tcaaggctgt cacacactgt gcaacgcgta tgcgttttgt tttgaatgat aataataagg
     8401 caaatgtcaa agagattgaa aaaatctctg tagttaaagg gacatttacc aatgctgggc
     8461 agtttcaggt aatcattggt aatgatgttc cagtttttta taatgacttt acagctgttt
     8521 ctggtattga aggggtgtct aaagaagctg ccaaatcagc agctaaaagt aatcaaaatg
     8581 ccttacaaca ggtgatgacc atgttggctg agattttcac acctattatt ccggcgatta
     8641 ttgttggggg gcttatttta ggtttccgta atattttgga gagtgtgcct tttgaatttc
     8701 ttgggcagca ggtcgaaaaa gggaaattag tttttgatgc agctggggat cctgtttgga
     8761 atacgattgt gagggtatct cctttctggt caggggttaa ccatttcttg tggttaccag
     8821 gggaagctat tttccacttc ttaccagttg ggattacttg gtctgtgacg cgtaagatgg
     8881 gaaccactca aattttaggg actgtccttg gtatctgttt ggtgtcacca caattattga
     8941 atgcttatgc ggtagcagga acgcctgctg ctgagattgc caaaaactgg gtttgggatt
     9001 ttggtttctt taccattaat cgtattgggt atcaggcaca ggttattcca gcccttttag
     9061 ctggtctgtc ccttgcttat cttgaaattt tctggcgtaa acggattcca gaagtggtct
     9121 caatgatttt tgtgccattc ctttctttga ttccagcttt gattttagcg catacggtat
     9181 tggggccaat cggttggact attggtaaag ggatttcctt tgttgtgtta gctggtttga
     9241 ctggtcctgt taaatggcta ttcggtgcta tctttggtgc cttgtatgct ccgctagtta
     9301 ttactggttt acatcacatg acaaatgcca ttgataccca attaattgct gatactgcaa
     9361 ctcgtacaac tggtttgtgg ccaatgattg ctctttcaaa tatcgctcaa gggtcagccg
     9421 tttttgctta ttatttaatg aatcgtcatg aagaacgtga ggctgaaata tcgcttcctg
     9481 cagcaatttc tgcttacctt ggggtaactg agcctgcctt atttggggtt aatgttaaat
     9541 acgtttatcc ctttgtagcc ggaatgatcg gatcaggtat tgcgggtctc ttatcaacaa
     9601 cctttaatgt tcaggcaaat tctattggtg ttggtggctt accaggtttc atggctatca
     9661 atgtgaagta catgattcca ttcttcatct gtatggtagt agccattgtg gtgccgatgt
     9721 ttttagcctt ctttttccgt aaatcacata tcatgactaa gacagaagat gaagctaaac
     9781 tacctgagac acccgtttcg gatgctcctg tagcaactgc tccacataag actatgcaag
     9841 gaacagttat cactttaaca agccctttaa cgggtgaagt taaagcgttg tctgaagctg
     9901 ttgatcctgt ctttgcacag ggagttatgg gccaaggtgc tcttcttcaa ccgacagaag
     9961 gggtgttagt agcgccttgt gatgctgaag tatcggtctt gttcccaact aaacacgcta
    10021 tttgtttggt gacgactgaa ggtttggaat tattgatgca tattggcatg gatacggtta
    10081 acttagatgg tcaaggattt gaagctttgg tgaagcaagg tgatcaggtt aaggctggac
    10141 aaacattgat tcaatttgat atagcagcta tttctgaagc tggatacgcc actgaaacgc
    10201 ctcttgtggt gactaatcaa gatgctttta cggtaactgt tgaaggtagt ttaccgcgtc
    10261 agattaaggc taatgataag ttagcagtag cgatgaaaaa gtaggtttac tagggagaaa
    10321 acgttcagga actgagatca gttcctgaaa ctattttagg gaggaaaact catgacaatt
    10381 gataaaaaga aagtcgtcta tcaaatttac ccaaaatcct ataaggacac gactggaaat
    10441 ggtgtgggag acttgcgagg gatcattgat aaattgcctt acttacaaga actaggaata
    10501 gatatgattt ggttgaaccc tttctaccct agtccacaac gagacaatgg ctatgatgtt
    10561 tcagattata cggcggtcaa ccctgatttt gggacaatgg ctgattttga agatttggta
    10621 aaagctgcta aggagcatca gattgagtta atgttggata tggttttgaa tcactgttcc
    10681 acagaccacg agtggttcca aaaagcttta gcaggagacc cttattatca ggatttcttt
    10741 atcttgagag atcagccgac tgattgggtt tccaaatttg gtgggaatgc ttgggcgcct
    10801 tttggagata caggcaaata ctacttacac ttgtttgatg tgacacaggc tgacttgaat
    10861 tggcggaacc cacatgttcg tgaggaattg gctaaagtgg ttaatttttg gcgagataaa
    10921 ggagtgaagg gattccggtt tgatgtgatt aatctgattg ggaaagatga agagctggtg
    10981 gattgtccgg tcaatgatgg taagccagct tatacggatc gtcctattac tcacacttat
    11041 cttcatgatc tcaatcaagc cagttttggt caggatgatt cgtttatgac agtaggggaa
    11101 atgtctgcca cgactattga taactgtctt ttatacacgg ctcccgaacg tgaggagcta
    11161 tccatggctt ttaatttcca ccatctaaaa gttgattatg agaacggtca gaaatggact
    11221 attatggctt ttgattttgc agcgctgcga gacttattcc atgcttgggg tgaaggcatg
    11281 agtcagggca atgggtggaa tgccttgttc tacaataacc atgatcaacc acgtgccctg
    11341 aatcgttttg ttgatgtaac acatttccga aacgaaggtg cgacgatgtt agcggcttcc
    11401 atccatttgt cacgaggaac gccttacatt tatatgggtg aggagattgg catgcttgat
    11461 ccagattttg atagtatgga tgattatgtg gatgtggaaa gtctcaatgc ttactcaagc
    11521 ttattagtct caggtaaaag tgcagaagaa gcctttgcca ttatcaaggc taagtcaagg
    11581 gacaatgcca gaacaccaat gcaatgggat gctagtgaac atgctggctt tacgactggt
    11641 aagccttggt tagaggttgg caaatcttat cgagacatca atgtcgaaac agaaaaagag
    11701 ggacgtattt tccctttcta ccaacgcttg attgctttgc ggaaggaact ccctattatt
    11761 gctgaagggg actatcgggc tgcttttaaa gatagtcagg ctgtctacgc ctttgaacgc
    11821 catttaggtg accagtgttt gcttgttctc aatcatttct atgctggtga ggtcgaactg
    11881 gaattacctc cacgttatca acatggacag gccttaatca gcaactatga gaaagtctct
    11941 atttgtgaaa aagtgatgct gaaaccttat cagacacttg ctattttagc tgataactaa
    12001 ctaaaagcct gaggttaggc ctcaggcttt tggtgattgt cacgaaaaaa gcagttttct
    12061 catatattta tttttaaact cttatttttt ctaaataaaa acaattgaga taagttattt
    12121 tagctaaatt ttggtacaat ggaaaagatt ataagtagag tttaagataa agattctaca
    12181 aatttaaaat gatataagga gatacgaatg acaacttatc aagatgattt ttaccaggct
    12241 gtgaatggaa agtgggctga aacagcggtt attccagacg acaagcctcg aacaggtgga
    12301 ttttcagact tagcagatga aattgaggca ttaatgctag acactacaga tgcttggtta
    12361 gcaggggatc atatccctga tgatgctatt ttaaaaaatt ttgtgaaatt ccatcgtttg
    12421 gtggctgact acgctaaacg tgatgaagtt ggagtaagtc caattctccc tttaattgaa
    12481 gaatatcagt ccttaaaatc tttttcagaa tttgttgcta atatagcgaa atatgaattg
    12541 gcaggtttac caaatgaatt tccctttagt gtggcaccag attttatgaa tgctcagctg
    12601 aatgtgcttt gggcagaagc accaagcatt cttttgccag atacaacata ctacgaagaa
    12661 ggcaacgaaa aagctgagga attgcgtggt atttggcgtc agtcgcaaga aaagctttta
    12721 cctcaatttg gtttttcaac agaagaaatc aaggatcttt tggataaggt aattgagcta
    12781 gataagcaat tggccaagta tgtcttgtct cgtgaggaag gctcagaata tgccaaatta
    12841 taccatcctt atgtgtgggc tgattttaaa aaactagctc cagagcttcc tttggatagc
    12901 atttttgaaa agattttagg ccaagtacct gacaaggtta ttgttcctga ggaacgtttt
    12961 tggacagaat ttgcagccac ttattactct gaagccaatt gggatttact caaggctaat
    13021 ttgattgtgg acgcggctaa tgcttacaat gcttacttga cggatgatat tcgtgtggaa
    13081 tcaggcgctt attcgcgcgc tctttcagga acacctcaag ctatggacaa acaaaaagca
    13141 gccttttatt tggcgcaagg accgtttagc caagcgcttg gcttgtggta tgctggtcaa
    13201 aaattctccc cagaagctaa ggctgatgtg gaaagtaagg tagcacgcat gattgaggtt
    13261 tataagtctc gtctagaaac ggctgattgg ttagcaccag ccacacgtga aaaagccatt
    13321 actaaattaa atgttatcac gccacatatt ggttacccag aaaaattacc agaaacctat
    13381 gctaaaaaag tgattgatga gagcttgtca ttggttgaaa atgcacagaa cttggctaaa
    13441 atcacgattg ctcacacttg gagtaaatgg aacaagccag ttgaccgtag tgagtggcat
    13501 atgccagccc acttagtcaa tgcttattat gatcctcagc aaaatcagat tgtctttcct
    13561 gcggcaatct tacaagagcc attttattct ttagatcaga gttcttcagc caattatgga
    13621 ggaattggtg cagtcattgc gcatgagatt tcacatgcct ttgacaccaa tggtgcttcc
    13681 tttgatgaac atggtagcct aaatgattgg tggacacaag aagactatgc tgcctttaaa
    13741 gagcgtacag ataagattgt ggcgcagttt gacggcttag agtcacatgg tgctaaggtc
    13801 aatggtaaat tgacagtttc agaaaacgtc gctgatctag gtggggtagc ttgtgcttta
    13861 gaagcggcac aatcagaaga agacttctca gcccgtgact tctttattaa ctttgcgacg
    13921 atttggcgta tgaaggcccg tgaggaatac atgcaaatgc tagctagcat tgatgttcat
    13981 gccccgggtg aactgcgaac aaatgtcacc ttaaccaact ttgatgcttt ccatgagacc
    14041 tttgatatta aagaaggcga tgctatgtgg agagctccga aagaccgtgt gattatttgg
    14101 taattaggta gttgataaac cttcaaatgc ctgtgattat aaaaatgact gattctgtaa
    14161 aacacaggat cagttttttg cttccttatt ggtgtccgtt ttgacacaac aaaaagacaa
    14221 ccctcaggaa agaaggttgc cttttttaga cttatcagaa ataagtgatt agttgttaag
    14281 ggcagcagcc atagtagctg caacttctga ttcaaagtcg ttagcttttt tctcgatacc
    14341 ttcaccaact tcaaaacgtg caaaagcaat cgctttagcg tttactgagt caaggtaagc
    14401 ttcaacagtt ttgctgtcgt ccatgatgta aacttgtgca agaagagtgt aagcttggtc
    14461 aactttagtg ttgtcaagca tgaagcggtc cattttacct gggatgattt tgtcccagat
    14521 tttttctggt ttgccttcag cagcaagttc agctttgatg tctgcttcag cagcagtaat
    14581 cacatcgtct gataattgag cttttgaacc gtatttcaag aatggaagag ctggtttgtc
    14641 aaccattgca cgagattcgt tgtcaagttc gatagcgtgg ttcaattgag caagttcatc
    14701 tttgatgaat tgtgcatcaa gctcagtgta tgaaagaaca gttggtttca ttgcagcgat
    14761 atgcattgat acttgtttag caagagcatc gtcgccgcct tcgacaactg agataacccc
    14821 gatacggcca ccattatgtt ggtaagcacc gaagtgttgc tcatctgttt tttcaatcaa
    14881 agcaaaacga cggaatgaga ttttttctcc gatggtagca gttgcattaa cgtaagcttc
    14941 agcaagagtt tcacctgaag gcataacaag tgcaagtgct tcatcgttgt tagctggttt
    15001 gccttcagcg ataactttag cagttgcgtt taccaattca acgaattgag catttttagc
    15061 aacaaagtct gtttcagcgt taacttcaac aacagcagca acgttaccat gaacgtaaac
    15121 gcctgttaaa ccttcagcgg caacacggtc agctttttta gctgctttag ccattccttt
    15181 ttcacgaaga agttcaacgg ctttgtccat gtcaccatct gtttcaacaa gtgctttttt
    15241 agcgtccata acgccagcac cagatttttc acgtaattct tttacaagct tagctgtaat
    15301 ttctgccatg tgtagtatcc tccaaatgat atttgttttg gtttcaaaaa acgagacaga
    15361 gcggtttgct ttctgccccg tttggttttc gattgttaaa gacaaacaaa gtttgccata
    15421 agagttagga actctgtgct aattaagcgt tgtcgccttc gacaacttca acgatttctt
    15481 cgattgaatc tgcttgaatg tcagcttcaa aagcaacgtc tgcatcttca ccttgacggc
    15541 cttcgataat agcgtcagct aatttagcgg tgattaattt aacggcgcgg atagcgtcat
    15601 cgttagctgg aatgatgata tcgatatcat ctggatcagc gtttgtatca accatcgcta
    15661 cgactgggat accaagtttt ttagcttctt taacagcaat ttgttcttta tgtgggtcaa
    15721 caacatacat cacgtctggg atacgaggca tatcttcgat accgcccaag aatttttcaa
    15781 gacgagcgcg ttgtttgtta agaagtgcaa cttctttctt aggaagaacg tcaaaggttc
    15841 cttcttcttc catacgtttg atttctttca aacgagcgat acgtttttgg atagttcccc
    15901 agttggtaag cgttccaccc aaccaacggt ggttgatgaa gtattggcct gcacgcgttg
    15961 cttcgtcagc aactgcttca gcagcttgtt tcttagtacc aacgaacaag atgacagcat
    16021 cgtttgctgc agcatcacga acaaattcgt aagcttggtc agctaatttt acagtttgtt
    16081 gaagatcgat aacgtggata ccgttacgtt ctgtgaagat gtatttagcc atcttagggt
    16141 tccagcgacg agtttggtga ccaaagtgaa caccagcctc aagaagttgt ttcattgaaa
    16201 ttactgccat gagtagtttc tcctttataa aatgtttttt cctcttccag acttcgactt
    16261 gcaggctaac ctagcggcaa caagcccaca attggtctag aatgagtatt tgccgcctac
    16321 acgaccctac tatattatca aaaaaagagg ttaaaagcaa ggagaaatgc tatggattcc
    16381 cttatttttg agatgactag cttctttgtt tattggtaag tgttaagaca agcttttgct
    16441 cattttgggt gacacttgcc aaatcgtttt atttaagata tagtgaactt gactaatttt
    16501 tggaggcgtt ttcatgacga agggtattcg atttcaattg cttggcagtc cccacatttt
    16561 tcttgatgac aaagaacaat tttttgcctt tgccaaagca aatgctctcc tttattactt
    16621 agtggttaat ggatctgtta gccgcgaggt ggcggcgagt ctactttggg aaaataagaa
    16681 cacgcaaaca gcgaaaaaga atttacgaaa cgctatctac caggtcaata aagtcttgca
    16741 agctgatgtg attatttgtc ccaatcgcaa cctgctggtt ttgaaaaaaa cacttgatat
    16801 caaaacagat attaaccttt ttctggctga tccgctagct cacttagaac tctaccaagg
    16861 ggagtttttg cagggctttt accttaaaag tggtgaggaa tttgatcttt gggtttcaaa
    16921 aatgcgtatg cagtatgaac aggtatacct aaaagcgtgt taccaaaaaa ttgaagaaaa
    16981 gctctcttta gatgccattg aagatgtcga ggaacattta aagcagctta ttgaacgtga
    17041 tgaatttgaa gagaaaaatt atcagctctt gatgaggctt tatcagcagg ggaattgtcc
    17101 tggtaaggtc atcgaaactt attatcagtt ggctaatgtg ttggataagg agctagggat
    17161 tcagccaagt ttgcagagcc aacagattta tcaggaagtg gtcgctaaag atcgtaatga
    17221 acgcaaaatc aagcattttt tgcgcaacag taatcatttt ttggggcgga ttgatgagat
    17281 taagcagctg gaaaactatt ttgccaactg tttggcctgc caagaggtag gagctctgct
    17341 cttaattggt gatacaggga ttgggaaacg aaccctagcc agacaggtct tagccaatca
    17401 aacccaaacg tttcaaattg tcacagctaa atgttttcgt gaagaggcta tggattcctt
    17461 attgccttgg cgtaatatct tagatggcct gggagatttg gtgattcaaa accgcttatt
    17521 gaccaccaaa gcttggaagg ctgctcttaa acgctgtttt cctgtggcaa ccatttttca
    17581 agaagataac aaccaaccct ttatcaagga ccacaccagc ttgttggttt cctttattgt
    17641 tgatatttta caacatttgg cagaaatcaa ggcgctggta atccttattg aggattgtca
    17701 ctggatggat gaggatagtt tgaccttgtt gcaacgggtc atgaatcaat tggtccacta
    17761 tccgattgct tttgtcttga ccaagcatct aggaacgact cctgagctcg gtctttgttt
    17821 gaatgctttg atgagtcagg ggcgtttaga aagcatttgt ttggagcctt ttaatcggca
    17881 agaaagtttg gcatatatta acagtcaact gggtagtcag ccagtgacgg aagaagaaat
    17941 ggagcacctc tatcaagcca gccaaggcaa ccctttcttt ttgtcagaat acactcagac
    18001 cctactgcgt cacgaaaaat ttgtgcctct aacgcctgcc attaaggcta agttgggtct
    18061 taaactagct aacctaagta gtcgtgatga cgcattgtta aactatttgt cttgttgtcg
    18121 gaggcctatc cctctaaata ccctcgctca attgatgtta ctgcctttag aagaagtgat
    18181 cgagatggtg gataatctgg ggcactacta tattttggtg gaagaaagcg taggagagga
    18241 agtgttgatt tcatttcgtc agcgaattat tcaactctat agttatgacc gtttatcttt
    18301 gtcaaaaagg cgtttattgc acggacaaat tgccaaacgg ttagaagatt tactgcctat
    18361 tttgacacca agccctcact tattagatga cattgcttac cattatcagg aatcacggca
    18421 ggtgattaag gctttggaat acaaccttaa ctatctagat gctaccttgc catttcagca
    18481 tgagctgttt cccatttatt caaagagtat tggctccttg gaaaagtcag atcgtgacca
    18541 tcaacgctta atggaagagc agtttgatag gattcgtcaa agcattgcgg atttggaact
    18601 gacctatgat aataaccgtg atttccagca gttgcttatt cgtttttctt accttgaagg
    18661 ccgttatgat attcgaacag ggaggtatca agaagggatt aaaaacattc aaaaggtcat
    18721 cgctttggca accgagctaa aacagccctc cttcttgcta gaaggctatc gccagttaat
    18781 tcattactgt atccaagtgg aaaacaagcc agaaatgcgt tattacacta gtctttcatt
    18841 ggaggcagcg gttgctgcta atcattttga ggccattgcg attagtttgc ggttaaacgg
    18901 tctttatcat ttgattattg gggagttaaa tgaggcagag cgtctgcttc aacagtcgat
    18961 tgacttcttt aaggtgacgc caggcttgca agctaactat gcgattcaga ttgctgctgc
    19021 cttagattat ttgggggaga tcgcccagat tcgctatcag tttgaaaagg ccgttgctta
    19081 ccaaaaacaa gccattgcct tgaccgaaaa caagccagct gagttatcag tgagtatttt
    19141 ttatattgga ttagggattt cctactttta tttagctgat tttgaacaag ctgagcaaat
    19201 cttgagtttg gctaaggaag ctttggtcaa tcatagttat ccttggaaag aaacgcaact
    19261 tcaaatttac ctggccatga ttcaatggaa aaagggcaat tatcagccag ccttgaccct
    19321 acttgattac agagaaacct tgatgtctag gtatcgcaat ccgcgtgata agggcttggt
    19381 tttctatttg atggctgtgg tcaagtacca attgatattt caaggagcta ccctaagtca
    19441 gcaggaaaaa gaaatggcag actacctgtt gtcagaatcc tttgagtatt attatgaaat
    19501 tgctagtact aacctcaacc cttaccgaga ttgtcatttg gtgagtgagt tgaacgattt
    19561 gcgtcaacaa ttatctgcta aaagctaagg aaaaccctct gattcacatc agagggtttt
    19621 agttatgttg tgccataatc tgtacgaggt aaaagataaa ggtggctgct aaattggcgg
    19681 tgtggttatc aatatcgtgt ggtggtgaaa cctcaaccac atcaaagcca actaattttc
    19741 cgctagctgc aatgtgttgc aaaactaaga cggcaagatt tgggtcaaca ccgagggact
    19801 ggatagcact aaccccaggt gcagcaccaa ctgagaaaca atccatgtcg atagtcaggt
    19861 agaccctttc ttgcccttct aagaaacggt caatggctcg gcagaccttt tgatggccca
    19921 tttggtagat gtcttgacct gttaaaaatt gaatgccctt ggattttgcc acaaagtcaa
    19981 ataggaaaag gttgttatta tgttcttgaa tgcctaaaac gaagtattta aagaggcgtt
    20041 tgtcagcaac tgcatcgtca aacatttgtc gaaagcctgt ccctgaatta gggcctgttt
    20101 ggtcgtaagg ccttaaatca aagtgggcgt ccatattgat gacagctagg tcatcacttg
    20161 gggataggct ttggcgtagg cctaagtagt ggccgtaggc ggtttcatgg ccacctccta
    20221 agacgatggg tttgagattg aggtcacaca tccgtttgat ggctttgctt aaactgtttt
    20281 gcagttgttc caaagagcga ttggggccgt caatgttacc cacatcgtaa accataactt
    20341 ggtttcctaa atgccaaggg aatttggcta gctgggttcg tatggcagct ggactttcga
    20401 ccgctccgac acgaccatta ttgatataaa cgcccttatc acttttaaag ccaatcaagg
    20461 caaaatgcgt cccttcaaat ggggttagtg agctgtcgtt aaggtcaagg aaagtcatga
    20521 ccatgcccca tttggcagta tataagtcat cgtcgatccc accatgataa tagctagtgg
    20581 tggatgggta gtaatcttct agcatgcgcg tcatctcctt taggattatg atagcaaaat
    20641 aaaacttaca gtcaacactt tcttggcctt tgtcactggt ttttaggaat agcagagcaa
    20701 taaaatcgtc tgcttttggt cccagttagt tagtctaaag aggccatgac ctctgccaca
    20761 gcccctctag ttaaagaagt accttattca ctaaaattgg atactcaact caactgcctg
    20821 ctcaaccgct tcgaggaagc ttgggtcttc aatgactgct gatgccttgt ttagttcatc
    20881 gtagatttcg atgtgtttat cgtgttcgat aaagttgatt tccttgcgga agagatcgta
    20941 ggcaacctta gtccctttac ctagttcatg attttcaggt ttgagatcaa gagcttggca
    21001 ggctgccatg atttcagtag ccacgatgcg gcgagaattt ttaaggattt caaaggcctt
    21061 gcgagcagcg gtagtgccca tgctgacaaa gtcttcttgg ttttcacaag atgggataga
    21121 atccacgctg gctggatgtg ccaaaacctt gttttcagaa gcaagagagg cacaagcgta
    21181 ctgggtaatc ataaagcctg aattgagacc tgggtatttg acaagaaagg atggcaattt
    21241 gctgagctgg ctattgacca aacgttccac gcggcgttca gaaacatttc caatttcaga
    21301 aatggcgatg cctaagaagt caaatggctg tgccattggt tctccgtgga aattacctcc
    21361 tgaaataacg tggccatctt tacagataat tggattatct gtgacagaat tgatttcaat
    21421 gtcaactttg gatttgacat aggcgatact gtctttgcta gccccatgaa tttgaggcat
    21481 gcagcgcaag gtgtaagggt cttgcacacg agattgagta gctacggttg tgttttggct
    21541 gccttcaatg aggtttcgga tattgcgggc tgttgccaac tggccgcttt gtgggcgaat
    21601 ggtgtgaagg ttttcttcaa atggactagt aatcccgtta tgcacttcaa gggatagggc
    21661 accagctagg tctgacagtt tcaataattg aatggcgtca taggttgcaa gggcaccgat
    21721 tgcagtgaga acagttgttc cgttgataag ggcgagtcct tctttagcgg ctaaagaaat
    21781 cttgtcaata ccagctttat caagagcttc ttgaccagaa agaagctctc ctttgtagta
    21841 agctttccct agaccaagca taggaagcac catgtgagca agcggcgcca aatctcctga
    21901 agcccctaga gacccttttt caggaatgta aggatgaacc cttttgttga gcagttccaa
    21961 cagcttttca atggtggata gacgaatacc agaataccct ttgacgagag agttgatacg
    22021 gatcagcatg atagctctca cggcatcttc tggcaatggg tcaccaaatc cactagcatg
    22081 ggtacgaatc aaattttctt ggagttggac tgtatcttct ggtgagatgc ttacgttaca
    22141 aagggatcca aaaccagtgg tcaccccgta aaccacccgt ttttcactga caatgtcatc
    22201 aacaatttta cgagaggcat tcacagcttc aatagcacta tcatcaatgc gacaggctac
    22261 gccttggcga gcaatcgcaa tcacgtcttc gatagtaagg ctttcgccat ctaaattaat
    22321 cactcttgtc atagtttttc tcctagattt gttccttagg gggcatttgg tccatttggt
    22381 ggttacgtcc gtgcataagg tagtacacac tacttgcaat gatgacacca actgcaccat
    22441 aaacaaagtt cattgggttg tcaccccaaa tcataactaa actaacgata actgcaagaa
    22501 ttgggataat aggaccaaat ggtactcgga agatcacatt agcatcggga tcgtccttcc
    22561 gtaatttcat aacggctagt gctgttggaa tgtattggaa gaagcggaaa acaacactga
    22621 gttttgctaa gttttcaaat gagccagtaa gtaggagtac aatggcaatt gctcctgaaa
    22681 ccaagatagc aacgagtggt gcgccatttt gattttgctt ggcaatagca gctggtaaga
    22741 gcccttcatc agcaatggca gcaccgtaac gtggcaccat gatggattct cccatgttaa
    22801 gacctgtaat ggaaattaaa gcaccgattg agaccatcca agcgccagca gggcctatca
    22861 ttttaacaaa ggcatcttga acaggtgcat tggtcatcat gatttgagag ccaagcatgg
    22921 caatggttcc accgataatc agcatatata agacagagac gatactgatt gatcctaaaa
    22981 gagcacgcgg tacatttttt tcaggggcac gcatttcccc agcgacaata gagagggttt
    23041 caaaaccgat aaagccatag aagatataaa cagcagtatt ggagatagcc cctaagaggt
    23101 tagtccctgg ttccaactga acaaatgggg taaagttagg aagcccgttt ttgatgaaaa
    23161 aaagggtaca ggcacaaaag gcaacgattg gaattaactt agcaatggtg gctgtgatgg
    23221 tgacaatttt agaggttttc aaaccagcga tattcatcaa agatagtaag atgattaacc
    23281 caatactaag tggaatgtgc caaccttcaa aggcagggaa agtaatgata aacattctgg
    23341 caaacccagc tgccatggct gcccaagcga agatagtaac ggtccaacca aggaagccaa
    23401 cgttaaagcc aataaaatca ccaaaagctc gtttggaata ttggaaggct ccgccgtttt
    23461 tcccaaaata accagagact tctgcaaaac aaactgccaa catgatggtc aaaatagctg
    23521 ttccaaacat aacagcgatg gatgcagggc caaggccctt gtaaatggcc ctaggtaaga
    23581 gaaaaatacc agagccgata acggcattga tcccgtaaag agtggcaccg ctcaagctaa
    23641 actttgcctg ttctctttct tgttcgttca tgtgatttga cgccataata atgctctcct
    23701 ttttgtttgc aaattgcgtt caaatataat ggataaggtc gctaggaaat gaggagatag
    23761 tcggtttagg agggtccttc ttgcacctaa cgatagtgat gagctattgg ttagcgatga
    23821 gaaataggta gccttttgtg atgatgtgta gtgatttcat tggagaagaa caggcatgag
    23881 tagtgtgatg cctatcttta tcccactaag tcttatacca taagcctgcc caaacagcta
    23941 ctggagtttg ggcggggctt gtttgattgg ggcaaacttt tagatctgaa aggctggcct
    24001 cctgctcgat taccaacaag tgattggctt caagctcttg attcgcctga ttggtcacag
    24061 aataagtcag tttgcttagg gcataaagca gttgacagtc cgctttgggt gcctcaaatg
    24121 gtgtgtcaga ccagacgggt aataattgcc cttggtattg gtcatcatac attaaattga
    24181 tgtcttggca gattccacga ctgacgattc tggtatcgcc ttcaaacgca tagggttcaa
    24241 aaggagttag ggaaatctct ttagcatgac tcaaatcact caatgtgatg ggcttgtcta
    24301 gggtcattag aatcctgtga tagccatcaa gtaaggtaaa ggttgtctgt cgtagggcca
    24361 cagttgactt tgagaggcga taagagaatt ctctcttttt gtaatctcca tcttcgggat
    24421 aaaggaacaa ttggttggtt tcaccgccag accagtcagt ttggaggtaa tcagagggcg
    24481 ctttgatagt tacctttgtc atttgttctc tccttgtttc caattagaat agtcctgtga
    24541 tgttgccctc ctcgtcaata tcaattgttt cgctggcagg tacttttgga aggccaggca
    24601 tggtcatgat agcacccgtc agagcaacga taaagcctgc tcctgcggac actttgaggt
    24661 tgctaatagt cactgtaaag tcagttggtg cgccaagttt tttggcatcg tctgagaagg
    24721 aatattgggt tttggccatg caaattgggt agttgccaaa gccgaggcgt tctaagtcag
    24781 ccagttcacg tttggctgcg ggggtcaatc taatcccctt accaccgtaa accttggtaa
    24841 cgattttagt cagtttggtt tcaatgctat catcttcctc ataaacaaag cagaattgat
    24901 tgtcttgctc ggctagtgcc acgacttttt cagctaattc acggccacca gctccgccat
    24961 ttgcccaaac atcagaaatc acaacgtcaa cgccacgttt gtcgcaggca tcatagacag
    25021 cttgtaattc agcatcggta tcaagcggga atttattgat cgccacgaca actggaagac
    25081 catagacgtc ttggatatta gccaaatgtt tgtcaaggtt aggtaaacca tccacaacgg
    25141 cctgaacatt ttcagtagcc aggtttgctt ttggcacacc cccatgcatc ttaagggcac
    25201 gtatagtggc tactaggaca acggctgctg ggcgaaggcc tgacatgcga catttgatgt
    25261 caatgaattt ttcagctcca aggtcagcgc caaatccagc ttcggtaacg gcataatcgc
    25321 catatttcaa ggctagtttg gtggctagga cactattaca gccatgggcg atattagcaa
    25381 atgggccacc gtgaatgagg gctggtgtgt gttccagggt ttgcacgaga ttaggatgaa
    25441 tcgcatcctt gagcagagcg gctagggcac caccagcttt caagtcttta gctgttacag
    25501 gttcaccttg gtagttgtaa ccgatgatga ttttttcaag gcgggctttg aggtcagaaa
    25561 tattttcgga taggcacagg attgccatga tttcagaggc gacggtaatg tcataaccat
    25621 cttcacgagg aaccccgttg accttgcctt gcaagccgtc aacaatatga cgaagctggc
    25681 ggtcattcat atccaccaca cgtttccaag tgatacgtct agaatcaatg cctaggctat
    25741 tgccatggtg aatgtggtta tcaatcaagg cagccaataa gttgttggcg acaccgatgg
    25801 catgaaagtc ccctgtaaag tgaaggttga tgtcttccat tgggacaact tgcgcgtggc
    25861 caccgccagc agctcctccc ttaaccccaa aaacaggacc aagagaaggt tcacgaaggg
    25921 caataactgc tttttttcca atggcagaaa gagcatctac caatccaacg gaagtagtgg
    25981 ttttgccttc accagctggt gttggtgaga tagcagtgac aagaatgagt ttgccatcag
    26041 gtttgtcttt taaggcgacc agttgacggg catcaatttt tgccttgtac ttgccataga
    26101 gacagagcgc ttcctcgtca atccctaatt gattggcaac cttactgata ggctccatag
    26161 tgacagagtt ggctatttca atatctgata aaaccatagt tgctccttta cttgttaaac
    26221 aatatcgaga attttcgtgt agatgtcatc tgccaagtga cagcccttgt ctaataaggc
    26281 ttgtcctttt tggcgatagt cggtaacaaa gtcttcatct ttgatgccag acagattaat
    26341 caaaacattc aaccaagcgc tttgtaaacc tgcttttaag tttaaagcag caactcctaa
    26401 gtcgctagca gcatttgtat tagacttacc gacagctgtt gcagtgatct caagtgcttc
    26461 gaccatgagg gtcatcatct caaaaggtga ttgtgcagct gttttcaagg ccttttgcat
    26521 ggcagttcgt ctagcagcct tgtcttcgtc agtctctttt ggcatatcaa aaacggcaga
    26581 aaccaggttg aaggcttcgg tatctttgtc aatggctgca agcaagctag cttgtagagc
    26641 ggtgctttta gcatggatct ctgtaataat agcttgataa tcagcatact ttttcttacc
    26701 aagggtcaat tcacagacca tcttggtcaa ggaaatgcca ttagcgccag agagagcagc
    26761 agcagaacca ccacccggag caggggcatc tgagccaagg actttagcga aatctgttaa
    26821 gcttaaatca actaaaccca tagagaacct cctaacctag caaatggttt tcaagaacct
    26881 gtttagggta gtcaaagtcc tcaacttgta agtagtattc agccacatcg attaaggcct
    26941 tggctggcgc taagccgatg acttcagaac caataacatt cacaccgtag cgacgcgctt
    27001 caaatttgat ggtttcaaaa gtacggtaaa gagaacattt ttcaaagtta accatgttca
    27061 tggagacttg ggcaatatgg cggtcttcta acatgacacc gatagcctta caatatttgt
    27121 agccaccacc tgaaccgcgg ataattttag caattttatg agcaatgtcg atattgtcag
    27181 tatccaaatt aacattgaaa gcaaccaatg gcatgcgagc accaacggca gttaccccag
    27241 cagttgggtg aatcttacga tctccgtagt caggtgccca atcctcttcc aatagttttt
    27301 ctggcatgcc ttcaaactga cctttacgga ttttagcaag gttttgacgt tctggacgtg
    27361 tggcagagtc ttcataaagg aagatcggaa tgtcaagttc acggttaatg cgttctgcga
    27421 cttgtttgga aatttcgaca cattcttgtg tagtgatgtc tttgattggg acgaacgggc
    27481 agacatcggt tgctcccata cgaggatgtt caccatgatg cttggtcatg tcaatgtttt
    27541 cagaagcgta tttcaccagt tggaaagctg cttcttgaat agactggtca tctccaacta
    27601 aggtaaacac gctccggttg tggctcgcat cagaagagta gtcgagaagg gtcactccag
    27661 gaatactttt ggccgtcgct accaaaccat cgataacagc ctgattttgg ccttctgaga
    27721 agttgggaat acattcaaca atttttgcca tgttaatctc cttttatgtc attgagttac
    27781 tcattagggg aataggttac ctactttaga taacgcttac atttttagct agcagttatt
    27841 agtgaaataa ttgatctacc gctgaagtca caaggtcatc atcagccaag tatgggatag
    27901 tgatgtgatc agtacctgca tggagacggt tgtattcaat agcggtttca atagcgtgct
    27961 catttcgggc ccagttacgg cgagctacac cgcccatagt atcccaagca atagcagatt
    28021 taataatctc atcaatgcgt tcgctgccat ctaaaacaag tccgaaacca ccattgatgg
    28081 ctttaccaat accagttccg ccaccgttgt gaagagccac taaactcata ccacgcgcag
    28141 cgttaccagc gtaacattgc accgccatat cacaggtcac attggaaccg tctttaatgt
    28201 ttgaggtttc acgaaatggt gagtcggtac cagacacgtc atggtggtca cgaccaatca
    28261 tgacaggtcc aatcttgcct ttgcggacca attcattaaa tttaagggcg acagtcacac
    28321 gaccgataca gtcttggtaa aggatccggg cttgggtacc aactacgagt tgatttttct
    28381 cagcatcacg aatccagttg tagttatcac ggtcttgata gcggcggtca ggatcgatag
    28441 cttccatggc agctttgtca gtagccacca agtcttcgtg tttaccactc aagcagaccc
    28501 aacggaaagg tccatagcca tagtcaaaaa gcataggtcc catgatatct tccacatagg
    28561 atggccagat aaatccgtct ttgtcgttac gaccattctt agaaatttct gtaataccag
    28621 agtcgtaaac agacttcata aaggcattgc cgtagtcaaa gaaataggtg ccattttcag
    28681 ttaacgtctt aatagcttca aaatgccgtg ccaaggtatc atcaaccatt tggtggaagg
    28741 tatctttatc ttctgccaag agccgggttc tttcgtcaaa gctaatacct actgggcagt
    28801 agccaccgtc ataaacgttg tgacaagagg tttggtcaga caaaagatca acatgaatct
    28861 gcttgtcatt gacgtactct aaaagatcaa cgatattacc atggtaagca atcgaagtgg
    28921 actctttagc atctatggct ttttgggcta attgcagagc ttcttcaggg ctctcagcaa
    28981 tttgggtaac ccagccttgc gaatgacggg tttcaatccg agattggtca acttcagcaa
    29041 taatggcaac tgctttagca atttcagcag ccttaccttg tgccccactc atgcctccta
    29101 aaccagatga gataaagagt ttaccggtca aatcaccatc gtcagccacc ccaagtttga
    29161 gacgaccagc atttaagagg gtattaaatg ttccatgaac gattccttgt gggccaatat
    29221 acatccaacc acctgcggtc atttgaccat agttggtgac acccatctct tcagcgattt
    29281 cccagtcttt catgttgtcg tattcaccca ccaaaaggcc attagtaata atcacacgag
    29341 gcgcttcagg tttggattgg aaaagaccaa ctggatgacc agactcaacc accaaggttt
    29401 gttcatcagt catgacttct aggtattttt taatcaaaca atattgcatc cagttggcac
    29461 aaacagaacc agtttctcca taagtgacta gttcgtaagg gtacaaggca atctcaaaac
    29521 tcaagttatt gtcaatcatg acctgcatgg ccttggcagc tgtacaattt cccttgtact
    29581 cgttaatggg tttgccgtaa atgcggtctt ttggacgcca tctgtaacca tagatgcgtc
    29641 cccttgtttt gagttcttcc aaaaattctg gaatgacttc ttcgtggaac ttcgtaggta
    29701 catagcgtag ggcatttttc aaggcgattt cggtctgagc ctgggtcaat cgaaaacctc
    29761 tgtctggtgc tcttcggata ccttctttga agacagtctt ttccggtaaa acatcgtcta
    29821 acttaacagt catagcggca gctatgtcag tttcactgta aaatgacata gaaaactcct
    29881 cctcaactcc tttttattgt cgagattagt ctaacttaga ctagtctata aaaagtcaac
    29941 cacgctttga aatgcgaaaa aagtctaaaa aaaaatcaaa acctcatttt ttgactaaaa
    30001 tttgacgctt aatcagtaca cttgaggcat gacagataaa gtcagtcaga gtttaattgt
    30061 tgttaccaaa gttgcagaga tcgcaactat tgagtgggtg aggggggact agtaatggct
    30121 attaatcagt tgacaaacga tgttaaaaaa ggaggttttt atggttgctg atgtcttatt
    30181 aacacatttt aatcaactat tttgtctaaa tgatccaggt catcctttaa ctggtcaaga
    30241 aatgaaaaaa gccactatcg ttgaagatgg ctatatagcc atcaaagacg gcctcattgt
    30301 ggctcttggt tctggtgaac cggatgctga gcttgtcggt ccacagacta ttatgcgttc
    30361 ttataaaggt aaaattgcaa cgcctggtat tattgactgt catacccatt tggtctatgg
    30421 tggcagtcgg gaacatgaat ttgccaagaa attagcaggg gtgtcttact tggatatttt
    30481 agctcaaggt ggtggtattt taagcacggt gagggccact cggtcagcca gctttgacaa
    30541 cctttatcaa aaatccaaga gattgctaga ctacatgttg cttcatggag tgactactgt
    30601 cgaagcgaaa agtggctacg gccttgattg ggaaacagaa aaacgccaac tggatgttgt
    30661 tgctgcgtta gaaaaagacc atcctattga tttggtgtct acgtttatgg cagcccatgc
    30721 gattcctgaa gaatacaaag ggaatcctaa ggcttatctc gatgtgatta tcaaggatat
    30781 gcttcctgtt gttaaagagg aaaatctggc agaattttgt gatattttct gtgaaaaaaa
    30841 tgtttttaca gctgatgagt cccgctatct gttaagcaaa gccaaagaaa tgggcttcaa
    30901 gcttcgtatt catgcagatg aaattgcgtc tatcggcgga gttgatgtcg cagcagagct
    30961 cagcgcagtt agtgcagagc atttgatgat gataacagat gatggtatcg ctaagttgat
    31021 tggtgctggt gttattggta atttgcttcc agccaccacc tttagtttaa tggaagacac
    31081 ttatgcccct gctcgcaaaa tgatcgatgc cggcatggct atcactctct caacagatag
    31141 taatccaggc agttgcccga ctgctaacat gcagtttgtg atgcaattag gttgttttat
    31201 gttacgttta acaccgattg aagtcttaaa tgctgttacc attaatgcgg cctactctgt
    31261 caatcgtcag gagagggttg gtagtttgac agttggtaaa gaagcagata ttgccatctt
    31321 tgatgcccca aatattgatt acccatttta tttctttgcc accaacttga ttcaccaagt
    31381 ctataaaaaa ggacaactca cggttgatcg aggtcgtatt ctttaaaaaa tttcaaaaag
    31441 aagctttgag cgttagctca aaacttcttt taaatatgct attttattga cgaatcaagt
    31501 agtcaaaggc accgatggca gcagtagcac cagaccccat ggaaataatg atttgtttat
    31561 aagctgaatc agtacagtct ccagcagcaa agataccagg aatattggtt gacccatgtt
    31621 tatccacaat gatttcaccg cggtcggtta ggttgacacc actatctttg agccaagctg
    31681 tgtttggaac aagaccaatt tgaacaaaga ccccttcaag gtcaaggtgt ttgtcttcac
    31741 cgctatcacg ctcagtgtag ctaagacctg taacatggtc ctctccaaca atatccttag
    31801 tagcaacatt tttgatgatg gtcatattgt tagtcttagc tgcgcggtct tgaagcactt
    31861 tgtcagcttt aagttcaggc aagaattcta aaacgtaaac gtgtttagca agaccagcca
    31921 agtcaagggc cgcttctaat ccagagttcc caccaccgat aacagcaaca tctttgcctt
    31981 caaagagagg accgtcacag tgagggcagt aagtcacccc tttattacgg aattcatctt
    32041 caccaggaac attaatgtta cgccatttgg ccccaagagc taagatagca gttttggctt
    32101 gtagaaccgc accattagct agagtaacct caatgttttc ttttttctca atagaagtag
    32161 ctagttgtgc tttgataata tcaacatcat aagacttagt atgggcctcc acttcggcca
    32221 ttaattttgg tccttcagtg taaagggtac caatcatgtt ttcgatgccg accgtttcca
    32281 tgacctgacc accaaaggtt tcagcaagta aacctgtttt tagtccttta cgagctgcat
    32341 agatagcagc gctattgccc gcagggccac caccaataac aaggacatca tagaggcctt
    32401 tatcagcaaa ggcttcctca gaaagtgggc cagcgatttg ttctaagagt tgctcaatgg
    32461 tagcgcgacc agaagtgaat tcttccccgt ctaaaaagac agtagggaca gacataatgc
    32521 cttttgcttt cacttcatct tggaacatgc cgccttctac catagtgtgt gaaatcttgt
    32581 cgttcaagac ggacataatg tttaaagctt gaacaacatc tgggcagtta tggcaggtta
    32641 agctgacata agtttcaaaa tgcaacggac ggtcaatggc cttgatgcga tcaataacat
    32701 cttgatccac ttttggagca cgtccagata cttgcaagag agcaagaata aaggacgtca
    32761 attcgtggcc taaaggaaga ccagcaaaga caaccccact accgtgacct tttttcgcaa
    32821 ccttaaagct tggttgtcgg tctaaagtga tgttttcaat agaaatacgc tcagacatag
    32881 cagctatttc ttccacaaag tctttgactt tttgagactg ctcattgtca ccaagggaaa
    32941 cttgcaaaac aagatcagct tctaacaggg tgaggtattg ggcaagttgt tctttaatat
    33001 caggacttaa tgccatagct ttcctcctaa aatttattaa attttaccaa ctaaatcaag
    33061 acttggtgtc aaagtttcag cgccttcttt ccatttagct ggacaaactt cacctggatg
    33121 tttacggacg tattgggcag cgtgaatttt atcaatcaag gtgctagcgt cacgtccaat
    33181 accatcagca ttaatttcca tcatttggat aataccatct gggtcaacga taaatgttcc
    33241 acgttgagca agtccgtctt cgccaagcac ttcaaaggct tgtgaaataa ggtgtgaagg
    33301 gtcaccaatc atagggtatg tgatagtgcc aaccacatct gagtcatcat gccaagcttt
    33361 atgaacaaaa tgagtatcag tagagacaga ataaacttct acaccaagag atttcagtgt
    33421 ttcgtattgc tcttgaaggt caccgagttc agttgggcaa acaaatgaaa agtctgctgg
    33481 gtagaaacaa aaaactgccc attttccttt aacgtcttca tttgtaacag tgatgaattt
    33541 tccatcgtga taagcttgag ctgaaaattc agcaatttct tttccaatta gagacatata
    33601 gttgtcctcc ttttttatag ttttatactt gtattataaa agaacaacaa gattatgtct
    33661 actcagaaac ccttgatttt actgggaaat cagcgattgt tttctgacaa ttcagttgtg
    33721 aaataaatca cattaattaa aatgattcta aataagctga agacgccgtt aaatcaaagg
    33781 ttaatttaat tataatctac agaaaatgta attgttatta aagcgtttac aaaaaaactt
    33841 ttttgattcc gttaaggcaa cttaatatta tggttaccta atcgctaata ttgtaaccta
    33901 ttttggcatg agatcaggga atggctgtta tattaattgg atatgtcttg tgcaaatagt
    33961 gcttgacgga tttgtggaat tagggtaaaa taatctttgt tgtggcggta tagccaagtg
    34021 gtaaggcacg gctctgcaaa agcttgatcg tcggttcaaa tccgtctacc gccttttata
    34081 tacaaaacaa gccttgatta atttcgtgaa aacgttgtta aatcaaggtt tttatttttt
    34141 gcgacaggat aaaaaaagag aaaaaaagaa ataaagtcgg tcaaaagtcg gtcaaatttc
    34201 ccgaagatgt cttttatagc ctgattatta ggtatgaaaa atactatggc caaaattggt
    34261 aaacgattgc aaataaaggt cagagttctt acccccttaa acgatatgcg ttaaaagtta
    34321 gttgaactgc cgtgcaccta acgatagaca cggtgatgtg agagcgagta gagttgaatc
    34381 tctacttgtt tatcttacca tagtcagctt tataaagtaa ccacccatta caatgcaact
    34441 atattcttaa tgtttttaag catgttattg acaagtgtta taaaatgtgt tactcttagt
    34501 gagtaaatta gtttttgagt tagtatttgt gtttcaacat ctttatttct ctctattttt
    34561 acaaatgatt tttagaaagg aagtggatgt atatggcaca aggtacagta aaatggttta
    34621 acgctgaaaa aggttttggt tttatttcaa ctgaaaacgg tcaagatgtc tttgcacatt
    34681 tttcagccat ccaaactaat ggttttaaaa cgttagaaga aggacaaaaa gtggcatttg
    34741 acgtcgaaga aggtcaacgt ggacctcaag cagtcaatat cactaaattg gcctaatctg
    34801 gttaaagtaa ctaaagagaa gcagatgttg gaaacaacaa ctgctttttt gatttctacc
    34861 agttaccttt aatgattaag agttgcgttt aaggtcttgt atttgttata ataatagtca
    34921 gttttagtca aaatgtattg acgtggtgct actaagtttt tagagtagcc cgcaagcaaa
    34981 ggagtgacag catgccaacc aaaaatactt cagacagcat tgaagagtat attaaagaat
    35041 tattagctaa gtcaggaata gcagaaatta agcggtccat gctagcagat tcgtttcaag
    35101 ttgtcccaag tcagatcaac tatgtcatta aaacccgttt tacggagagt cgaggctatg
    35161 aagtagagag taaacgaggt ggtggaggct atattcgtat tgccaaggtt catttttcag
    35221 acaaacacca tctgattggc aatttaatgg caactattga agactgtatc agtgagcaag
    35281 ttttcacgga ctcgatacaa ttgctttttg acgaacactt attgacagag cgtgagggaa
    35341 atatcatttt ggctgtggcg tctgatgatg ttttaggaac tgatggttca actattcgtg
    35401 cacgaatgct ctatcgctta ttgcaacgaa ttgacagaaa gggaagcaat taatgattat
    35461 gtattcaacg aagatgcaag acatttttag acaggcgcag ttccaagctg ctcgctttga
    35521 tagccattgc ctggaaactt ggcatgtttt gttagctatg gtagctgtag ataattcttt
    35581 agcaaatatg attttaagtg aatatgatgc ccaagtcgcc atagaagaat atgaagctgc
    35641 agctatttta gccatgggca aaacccctaa ggaacagttg tctcgtgtag acttcagacc
    35701 tcaatctaaa accttgacta acttgttagc ttttgcgcag gctattagcc aaatcactag
    35761 ggatcaagaa gtcggctctg agcatgtctt atttgctatt ttattgaatc cagatatcat
    35821 ggcgagtcgt ttgttagaaa tagcaggcta tcagataaaa gataacggca atgggcagcc
    35881 gcgattagct gacttgcgaa aagcaataga acgtcatgca ggttacagta aggaaatgat
    35941 taaggctatt cacgaactac gtaagcctaa aaaaacgaaa acacaaggga ccttttcaga
    36001 tatgatgaag ccaccaagta cagctggtga attgagtgat ttcacaagag atttgactga
    36061 aatggcaaga caaggtttgt tagaatcggt gattggacgt gaccaagaag tatctcgtat
    36121 gattcaggta ctaagtcgta aaacgaaaaa caatcctgtc ttggtaggtg atgcaggtgt
    36181 tggtaaaact gcgcttgctt atggccttgc tcaacggatt gcaaatggcg ctattcctta
    36241 tgaacttaag gagatgcgtg tcctagaatt agacatgatg agtgtggtag caggaacccg
    36301 ttttcgtggg gattttgaag agcgcatgaa tcaaatcatt gatgatattg aagctgatgg
    36361 tcagattatt ctttttgttg atgaactaca tactattatg ggttctggca gtggtattga
    36421 cagtacactt gatgcggcta acattttaaa accagcatta tcgcgcggta ctcttcatat
    36481 ggttggagca acaactcaag aagaatatca aaaacatatt gaaaaagatg cagctctttc
    36541 gcgtcgtttt gctaaaatat taattgaaga acctaataca gaagatgctt atcagatttt
    36601 gatgggccta aaattatctt atgagaccta ccataatgtc tcgatatcaa atgaggcagt
    36661 taaaacagct gtaaaaatgg cacaccgtta tttaaccagt aaaaatctcc ctgattcagc
    36721 tatcgattta ttagatgaag ctagtgctgc tgtgcaaaac atggtgaaaa aatcagctcc
    36781 tgagacttta acaccaatag accaagctct tatcaatggt gatatgaaaa aagtatctcg
    36841 cctcttagct aaagaagcaa aaggtcagat gagaaaacca acaccagtga cagaagatga
    36901 tattttggca accttgagta agttatcggg aattccactt gaaaaactga cgcaagctga
    36961 tagtaaaaaa tacctcaatt tagaaaaaga actgcataag cgtgtgattg gtcaggatgc
    37021 tgctgttacg gctatttcaa gagccattcg tcgcaatcag tcaggtattc gaacaggaaa
    37081 acgtcctatt ggatcattta tgtttcttgg cccaacagga gtaggtaaga cagaactagc
    37141 aaaggccctt gcagaagttc tctttgatga tgaagcagcg cttattcgtt ttgatatgtc
    37201 tgagtacatg gaaaaattcg cagcgtctag gcttaatgga gcacctcctg gttatgtcgg
    37261 ctatgatgaa ggaggtgaac tgacacagaa agttagaaat aaaccttatt cagtcttgct
    37321 ttttgatgaa gtggaaaaag cacatcctga tatttttaac gttctccttc aagtattaga
    37381 tgatggtata ttgactgata gtcgtgggcg taaggtcgat ttttcaaata ctattattat
    37441 catgaccagc aatcttggcg caacagccct gcgtgatgat aaaacggtcg gttttggggt
    37501 caaaggcatt caccaagacc atcaagctat ggagaaacgt attttagaag aattaagaaa
    37561 aacttaccgc ccagaattta tcaatcgtat tgatgaaaaa gtggtctttc atagtctgac
    37621 ccaagataac atgcgcgatg tggttaaaat catggtacag cctctgatta ctacattggc
    37681 agaaaaaggt attaccctta aaattcagcc tttggccttg aaacatttgt ccgaggtcgg
    37741 ctatgatgag catatggggg caagaccatt acgtcgaacg ctgcaaactg agatagaaga
    37801 taagctatca gagcttatcc tttctcgaga attgacaagt gggcatacgc taaaaattgg
    37861 attatcatat ggcaaattaa cgtttcacat agcttaattt ccatataaaa ggtggatcat
    37921 ccttgatgtc accaaaaaaa gaggttattc ttacaaaaat aacttctttt ttcttgacta
    37981 tttttgacca agtgataaaa tagaatataa agttagcact cgatgaacat gagtgctaac
    38041 tttactaaag attcttttat tggaggaaaa tttcatgtta aaaccattag gtgaccgtgt
    38101 ggtcgtaaga tttgatgatg aaaaagagca gacagtcggt ggttttgtcc ttgcaggcac
    38161 tcataaagag tcaacacgaa aagcgacagt cctcgctgtc agtgaaacgg gtgttcgcac
    38221 tattacaggt gatagcgtgc taccttcagt ttcagtgggt caagaggttt tagttgaaaa
    38281 tgggcatgat ttagaagtaa ccgtagatga tgagaaagtt tctatcatcc gtgaatctga
    38341 catcattgct attgttacaa aataaaacga caaatctcta aatagaaaga aggattgaat
    38401 atggcaaaag atattaaatt ttcagcagat gcgcgtgctg ccatggtgcg cggagttgat
    38461 atgttagcag ataccgtcaa agtaacgctt ggtcctaaag ggcgtaatgt tgttcttgaa
    38521 aaagcttttg gttctccctt aattactaat gacggggtaa ccattgctaa agagatcgaa
    38581 ttagaagatc attttgaaaa catgggagca aaattggtgt ctgaagtggc ttctaaaacc
    38641 aatgatattg ctggtgatgg gacgactact gcaacagttt tgacacaagc cattgttcat
    38701 gaaggactaa aaaatgtgac agcaggtgct aatccaattg gtatccgtcg aggcattgaa
    38761 acagcaacag caacagctgt tgaagccttg aaagccattg ctcaacctgt atctggcaag
    38821 gaagctattg ctcaggtcgc tgcagtatca tcacgctctg aaaaagttgg agagtatatc
    38881 tcagaagcta tggagcgtgt gggcaacgat ggtgtgatta ccatcgaaga atctcgaggt
    38941 atggaaacag aacttgaagt ggtcgaaggc atgcaatttg accgtggtta cctgtctcaa
    39001 tacatggtca cagacaatga aaaaatggtt gcagaccttg aaaacccatt tatcttaatc
    39061 acggataaaa aagtgtcaaa catccaagac attttgccac tacttgagga agttcttaaa
    39121 accaaccgtc cattactcat tattgcagat gatgtggatg gtgaagccct tccaaccctt
    39181 gtcttgaaca agattcgtgg tactttcaat gtggttgctg tcaaagcgcc aggatttggt
    39241 gatcgtcgta aagctatgct tgaagacatt gctatcttga caggtggtac agtgattaca
    39301 gaggatctag gacttgaatt aaaagatgct acaatgacag cccttggaca ggctgctaag
    39361 attacagttg ataaagatag cacagtaatt gttgaaggtt caggaagttc agaagctatt
    39421 gctaaccgta ttgcactgat taaatcgcaa ttagaaacaa caacttctga ctttgaccgt
    39481 gaaaaactac aagaacgttt ggcgaaatta gctggtggtg tagctgttat caaagtagga
    39541 gctccaacag agacagcttt aaaagaaatg aaacttcgca ttgaggatgc tctaaatgcc
    39601 acacgtgcag ccgttgaaga aggtatcgtt gctggtggtg gaacagcact tattacggtg
    39661 atcgaaaaag tagctgctct tgagcttgag ggcgatgacg ctactggacg taacattgtg
    39721 cttcgtgctc tagaagagcc tgtacgtcaa attgctttaa atgctgggta cgaaggctcc
    39781 gtagttattg acaagttgaa aaacagccct gcaggaacag gatttaatgc tgcaacaggt
    39841 gagtgggttg atatgattaa aacaggaatc attgaccctg tcaaagtaac acgatcagcg
    39901 cttcaaaatg cagcttctgt agctagtctt attttgacaa cagaagcagt tgttgctaat
    39961 aagcctgaac cagctgcgcc agcgccagca atgccagcag gtatggatcc aggaatgatg
    40021 ggcggcttct aagctctcta tagcattaag gattatgaaa aagaagttga gactaaaggt
    40081 ctgcttctta ctatgaaaaa agaacaaaga ttggatttcg actcatgata tcccatcttt
    40141 gtttttttag ttgtgatata aaatgccaat ctttgtggtt acagactttt aatgtattgg
    40201 aaaatatatt gtttttcttt gtcatgtgct atcatggagc caatgaaaag ttattcccat
    40261 atatgcgtgg tggaggtctg tgatgaatca ttttggagag atttttaaaa cctttagaga
    40321 atcaaaggga ttacgattaa aagatgtcgc aaaggctggt atatcaacct ctcagctgtc
    40381 gcgtttcgaa aaaggggaga cggatttaac catatcgaca tttatgctga ttttggatga
    40441 aagtaacatg tccattgatg agtttatgta tgctgtccat gattttcatc gtgacgactt
    40501 aaatgaactc ttatcaaaaa gtgagggatt tcgtaacaat tcaagatagt gatggcttga
    40561 agcagttact tcatgctcaa ctagcatcaa ctgataagaa ggaaatgttc acggatatct
    40621 tttggcatat ctagcatgtc taagtctgct atggcatgcc attttgggag tccctcacgc
    40681 gactctgtca gtagctctcc ttcaaaggat tcacatagaa agtcgtaata gacatagcac
    40741 tctttttttg tcaggcttga taaagctgga aatacccttt aattgaaggt ttaaagctgt
    40801 cagacctatt tcttctttta gttctcataa agccgcttca aagaaagatt cacagggctc
    40861 gactttacct tctggctgga cccatcctgg taagttatcg tgttgtctat ggagtcataa
    40921 aatcatatcg ttatgtttga cacacatatt gccccagtct ctaaccgttg ctatcaccag
    40981 attcaccacc ttttagtata agaaaaaagg ggtgaaagag gtttcttgtt gtttttttgg
    41041 cttaagattt ctttaagtct tatctagtat actaatgtca ttccttaata ggataagtat
    41101 ttggttgtag aagtatcgtc ttaaactaac gctatctcaa ccgattccca acttattcac
    41161 ccctcctata ccgataaggt ctcacaaagt gtgagacctt attttttgac ttcagctcta
    41221 gttatggaga aacttgttga tacaagcttt tttgggttat gggttatggt attatgatct
    41281 attcaagcct ttcttgtttt ggggtttttc ttcaaccaac ttggcttcaa tagcttttaa
    41341 gcgtttgaag gttttttcag cgcgatctag tgaaatctta tcagcttttt cttgagcagc
    41401 tttggggtca ctgacgacta agtcactgat ttctttggtg cttttgtctt gttcggctat
    41461 ccaggttttt tccaagcctt tcatctcatc aatcacttta gttccaaatg gttttggatg
    41521 agcagccacg agatcattaa tgtgtgacac cgtccaatac catgacttat cgttatattg
    41581 ggtgcttttt tcttgatagg cttcataggt tctactgata ttgccaaggt atggcaagta
    41641 aggggcatta cgaggactgc caatagatag ccacatgaca ccaccaccca attcagcagg
    41701 aatgtctttt ttcagttgga aaatatgagc ttccatgaca ttaggatttg agatggggta
    41761 agcataacct ttaacagctt ttttggattt tggtttaccc ttaccgtcta atgccatctg
    41821 atcaagtggt ttaagatcca gcccctcaaa acgattacgt tgcagtttca tcgcatcttc
    41881 tagactaaag gtcttatcag tgctttggag caactcataa ttgctgtctt tataagttac
    41941 ttttgaatca gggtcaagtg atttaatccc tgagaaagag cgagagcggt tggcatcgtt
    42001 tagtggtgga ttgtaagatt tggcaatatg aaatttacca tcaacttcgg tataagattt
    42061 ggctttttta gtgacttttt ccacatcttc tgaggcaatc gtattttcct tatcgttgaa
    42121 atcgacatgt ccgagataga aggtatttgg aaaaacagcg tatttatcat ctgggaattt
    42181 gatggcaacg tattggtgac cagagagaat ttccatgtac cagatgccat ctttgtcagc
    42241 caaggtaaca atatttcctt cagcagctcc tttttcagtg actattttag caatcaatgc
    42301 gacaccttca cgggctgttt tgacacttgg taaaatgaca ctggccatag atgattccgc
    42361 taagccattt ttgacgtaag gatcaatttt ttggatggca tcattagctg aagctgatac
    42421 tgttgcagac atagagacgc caaactcatt aaaaccagct tcgtcataaa cgcctttatt
    42481 aggtgttaca tcaggaatcg cactatagcg ataagaatgc tcaggcaatg ggtactcaaa
    42541 accattagag gggtctttcc atttttcgcc agcaggattg tcctttgcta gacgtacaat
    42601 gaagttttta ttatgatgtg gctcaagatc ttctgttcga ccataaagaa gagaaccgtc
    42661 tttggttaaa tctttgccaa taataaaacc agtacaagca taactaacac tttgaagcga
    42721 aaaggctgtc agaatactta aaactccaag tgatattttt ttgtttatca tgcgaatcct
    42781 cctagtaaac gataacgctt tcacagacat tataaaatgc tctttttaaa aattcaagtt
    42841 ttttatgaag tgttttgaaa ttgttagcta tctaaatatt atgagaattc tctcttgaat
    42901 tttggcttaa cggcccctaa aaacgtatag gcagtgaact atctaggttt cttcgaaaat
    42961 ccattagcca tttatgatat aatagtaaag aaaaacatag aaagttgggg tctgttgatg
    43021 accaaaccat ttcatcataa gaaactaaaa caaataacta taatagctgc aactagcctt
    43081 tttttattcc tgatcggtgg tgccttttac tactctaaaa atcattgtat taatgcctat
    43141 cttaaagctc gatcagccca atcaggacca gtttttgaaa atatcaaagc atatctagtc
    43201 tgggatgata ctaatgagca gatcacaaat gacgaggcga tgtatactaa gtttagaagg
    43261 tatagtcaaa aagaattgag gcaaaaaaag caggatttaa aagccgctag tcaagatagt
    43321 gccgttcaag taaagtctgt tggtcgtcgt ttttggattt ttcctgatta tcggattgcc
    43381 ataaaaccca tggatttaac gataaaaacg aatgtgcctc aagcagacgt tcttttaaat
    43441 caaaaaaaag ttgctgtttc tgattcagaa cagttctcag taaagcttga tcggctacca
    43501 acggcagaat ataccgcaag tatcagaggc aaacacaacg ggcgaaacat taaagtcaac
    43561 aaatcatatg atggtgataa tcccgtgcta gatttgagtg tgtcttttag aactttttta
    43621 gtaacaagca atgctaagca aggagatctt tacttcgatg ataaccatat tggcacatta
    43681 aaagatggtc aattacaagt agaagattac cctgttacag aaaacgcaca agcttatatg
    43741 aaaacaacat tcccagatgg tgagttaaga tcacaaaaat atgctctagc tgatgttgaa
    43801 gaaggagcaa ccctggagat tttagttaca gatcttttag aagaggacaa agcaggggag
    43861 ctattagtat cagcttttga tcagctgatg cactacctca gtacaggtca agactcatct
    43921 aacttacgta gcgtctttga ggcagggtct agcaacgcat tttatagggg attgaaagag
    43981 tccataaaag cgaaatttca gacagataca agaaaagcca gccgtctcaa tattccatct
    44041 atccttttga caacaatgac tcaagtgggt aaaacaactt acgtgcttga tttcacagct
    44101 acctacgaat ttttgtatga caactcaaca gatcctgagc agcatacctc tggacatatt
    44161 aatcaagact tgactggaaa agtgactgta aaaaaagttg gacagcatta ccttatcagc
    44221 cagtccggct ctaaaaatat tactgttgtt aaagaggaca atcaactcaa agcgccatct
    44281 gtctttcctg agtctatttt gggaacgtgg acaggccaag ccaatggttt aagcatccat
    44341 atgtctctag catcagatgg aacaattacg actaaagttg aagatcgaaa aggcaaccgt
    44401 tctaaagaaa ctcggacagc taaaattagt aaagttgaag acaaaggcaa tggtttttat
    44461 ctttatacgc cagatcctgg aagtgacata agcgccttag ctccagaagg aggattgggg
    44521 ggtgcaaatg tcaaatatgc ttatggtttc aaaatatctg gtaaaacagc ctctccagtg
    44581 gtgtggcagg cagcattaac acatgaattt gattatacca agccactttc gggagtaact
    44641 ttgcaaaagc aaccataaag gatgcaaagc cacaaagaac agtagaaaca atgttatcac
    44701 tcaaatacag tgctatgaaa acagataaac tcaaaaggct aggagattat ttcctagcct
    44761 tttgagcgtt taagactgcg tcaaatgtag ctgatttagc ttgcacgcaa atgtcttctt
    44821 gggttagagg gtgttttaaa cgtaattgat aagcatggag catcagtcga gcacaatctt
    44881 tttttccatt agaatacaaa ggatcaccaa ataaaacatg accttgatgg gctaaatgga
    44941 ctcgaatttg atgagtccgt ccagtttgta attgacaatt gagtaagctt gccgttttgt
    45001 ggaagttttt gactagagtt acctcagtaa tagctttctt gccatttatc ggatcgacaa
    45061 cacgttttct gcggtcatgt cgatggcgac caattggatg atggtaagtt accctaggac
    45121 tgtctaagga tccgtgaact agtgccaaat attctcgatg gatgtctctt ttttctaaaa
    45181 gacgattaag gattggtaag ataaaaggtg ttttagcaaa taaaatggcc ccacttgttt
    45241 ccttatccag acgatgaacc acatagcagg tctgtcccgt gtaagcagac acgtggttga
    45301 gcagcgccaa ctctgttgga tcattgccgt gtgttttcat cccttcaggt ttgttaacaa
    45361 taatgatgtg ctcatcttca taaaggcagg ttactttttc ggcctgcccc atcacgataa
    45421 tcttttcagg ataatcttcg tgatcgaaaa aaagtttgac ctgatcaccg tatttaacac
    45481 aactttgcca gttaacagga tgaccattaa taagaacatg ttttttagtt ctcagaaaat
    45541 gcctaatttt acggggaatt aacaattgtt cctctaataa tgctttgacc gtcaattctt
    45601 caaagggatt tttcagtgtg acagtaaacc cctttgatgg tttatttgtt gtcttcattg
    45661 agccagttat ggtatttttc aacaaggttg atagccattt gggtggcaag agcagcagag
    45721 aaagcttcag taccttcacg ctcgtcatta gctacctcca agcgagtctc atcgtaaatg
    45781 gcttttagac tttctgcgga taaggtttct ttaaaagctt ggaatttatt cattggttaa
    45841 acccgtcctt ctatgtttaa tgtgagctcc tgattaatgc aaggaattca atgctattat
    45901 agcatgtttc taatcaaaac gtgccactca aaaaggtaaa aacgttattt tgctaatcca
    45961 tctaataaga aagtgtctta aataagacct cttgatagag tttgtaacag aaatgatatg
    46021 aaactaggtg ctaacgcaga aatagaaaac aactaacgtg ttgcgaagtt ggaacagtca
    46081 taaaaaaata gcaagataaa acttatcaac acacagttac ctaagacgat gagtcaaaca
    46141 ggtatgttct ttcaaaacaa ccccatgagg ttgaacgacg tatcggaaaa atacggatta
    46201 gtttggcctt atgataggtc cttctctatc gtaaaagaag tcaaccaaag gtttttaata
    46261 taaaatatta agaaaaattg ttataattat gataaaaaca taatcgtaaa atagaggaaa
    46321 aacaatgaaa acaattagac gttatgatgt taacgaagat agagggcata caggccttgt
    46381 agaagcagga gatttctatt atttaaacta ttgtgtcggc aatgttggac aagacattga
    46441 aagtcagatt aatggagcct ttgacgagat ggagcgccga ttagcattgg tggggctcac
    46501 attagatgca gttgttcaaa tggattgtct atttcgagac gtttggaaca tacctgtgat
    46561 ggaaaaaatg attaaagagc gctttaatgg cagatatcct gctcgaaaat cgattcaaac
    46621 ggaatttgca catcacggtg gacctcaagg actactcttt caagtggatg gtgtggctta
    46681 ttcaaaacat atttcgatga cctaatcaag gttactttaa gatcatagtg gtataataac
    46741 gttatgagat ttctagaact tttacaaaag aaattttttc ctaaagcata tcaggaaaaa
    46801 caattcttaa tgcatcaaaa aacgcgttta acgccacaac acaatcaaaa gcagtattcg
    46861 ccaaatgcca atcatttgga ctcatcagct accaaaaact cagaacaaga ccctgcaata
    46921 gctctgcaac gcagtagagc ctatgaagga agccctaaaa gtcggcccgc ttggttgcaa
    46981 aagttggaag ctgttttgcc gtctcctcaa catccaattc ggcgtttttg gcgccgctat
    47041 cacatcggaa aactgctaat gattctgatt ggaactcttg tcttactctt aggatcatac
    47101 ttgttttact tatcaaaaac agctaaagta tctgatttac aagatgcctt gaaggctaca
    47161 actgttattt atgatcacaa aggagagtat gcaggcagtt tatctggtca aaaagggagt
    47221 tatgttgagc tcaacgctat ttcagatgat cttgagaatg ctgttattgc cactgaggat
    47281 aggacttttt acagtaatag cggtattaat cttaaacgct tcttattggc ggtagttacg
    47341 gcgggccgct ttggaggtgg ctcaacgatt acacagcaac tggctaaaaa tgcttatctc
    47401 tcacaagatc agacaattaa acgaaaggcc cgagagtttt ttttggcgtt agagttgacc
    47461 aaaaaataca gtaaaaaaga tattcttact atgtacctta acaactccta ctttggaaat
    47521 ggagtttggg gagttgaaga tgccagtcaa aaatattttg gaaccacagc tgctaactta
    47581 acactggatg aagctgccac attagcaggt atgctcaaag gacctgaaat atataaccct
    47641 taccattctc taaaaaatgc tactcaccgt agagatactg ttttaggagc gatggttgat
    47701 gccaaaaaga ttacccaaac aaaagctcaa caagctagag cagtagggct aaaaaatcgc
    47761 ttagctgata cttatgttgg taagacagat gactacaaat acccatccta ctttgatgct
    47821 gttattagtg aagcaatagc aacttatggt ctttcagaaa aagacattgt taataatgga
    47881 tacaaagttt acaccgagct agatcaaaat taccaaactg gcatgcagac gacttttaac
    47941 aacgatgaac tatttcctgt ttcagcttat gacggtagct ctgctcaagc agctagtgtt
    48001 gctttagatc ctaaaacagg aggtgttaga ggtctgattg gtcgtgtgaa tagtagtgaa
    48061 aatccgactt tcagaagttt taactatgcg actcaagcaa aacgtagtcc cgcatcaaca
    48121 atcaaaccac tcgtggttta cgcgccagcc gttgcttcag gatggtcaat tgaaaaagaa
    48181 ctaccaaata ccgttcaaga ttttgatggc tatcagccac ataattatgg aaattatgaa
    48241 tcagaagatg ttcctatgta tcaagcatta gcaaactctt ataatattcc agcagtttct
    48301 acattgaacg atatcggaat cgataaagcc tttacctatg gtaaaacatt tgggttagat
    48361 atgagctctg ccaaaaaaga gttgggggta gctttaggtg gcagcgtgac aaccaatcca
    48421 ttggagatgg ctcaggcata tgctgccttt gccaataatg gagtaatcca tcctgcgcac
    48481 ttgattaacc ggattgaaaa tgccaggggt gaagtgctta aaacctttac tgataaggct
    48541 aaacgtgttg tcagccagtc tgttgcagat aagatgacag ccatgatgct aggtaccttt
    48601 tcaaatggaa cagcagtcaa tgctaacgta tatggctata cactagctgg taaaacagga
    48661 acgacagaaa ccaacttcaa tcccgactta gcaggcgatc agtgggttat tggttatacg
    48721 ccagatgttg ttattagtca atgggtagga tttaatcaga ccgatgaaaa tcattatcta
    48781 acggattcaa gtgcaggcac ggcttcagct atttttagca ctcaggcatc ttacattttg
    48841 ccttatacca agggcagcca atttcatgta gataatgcct acgctcaaaa tggtatttca
    48901 gctgtttatg gagtcaatga aacaggtaat caatcaggag ttgacactca atctattatt
    48961 gatggtttaa gaaaatcagc acaagaagct tcgcaatcac tatcaaaagc agtcgatcag
    49021 tcagggttac gtgataaagc ccaatctatt tggaaagaga ttgttgacta ttttagatag
    49081 cttgcagtat tttagagatc gtgttaaaat aatagttacg gaggcgttat ggcacagaaa
    49141 aaagcgagtc tagcgtgtgt cgaatgtggc agtcgtaact actctatcgg agtgagtagc
    49201 acgcctaaac caacacgtct agaagtaaat aaattttgca aatactgcaa aacatatacc
    49261 ttacacaaag aaacacgtta aaaaggagag cctgatggga ttcattagcg ggacttttaa
    49321 agtcttaaaa gacacaacat ggccaaatcg taaacaacgt tggaaagatt ttatctcagt
    49381 tcttgagtac acagctttct ttacggtgat tatctacatc tttgaccagc tcttagcaaa
    49441 gagtgtatta gcactgatta atttatttta ggtcaagatt ggtagaaatg ataagcttgc
    49501 ttggaaaagc ctaggctggg agtattccca gccttttttg tagtatcaat aatctaatta
    49561 gtaagctttt ttaaagccca tactggtttg agctattcaa aagcataaag gtaaaggcaa
    49621 tgtagcaacg atctccaaac caagttcact aaattagctt gtaacagagt caacgcataa
    49681 acagcctggc ctcacttgct tagcacatta aaaatagcaa tgaatattat ggttgaggaa
    49741 aacagcctga tagtctgcta aatcttcggg atgataagcc ttgacctcag ccatctcata
    49801 ctccctacct agaagtttgt acttgttttc tccaaattga tggaaaggta acagttgcac
    49861 ttggtcaatc tcaagctgat taaagagctc tgagaaggct ttagcatcgt ctagtgaatc
    49921 attaaattgg ggaataacag ggatgcgcaa gacgatttct tttccagctt gaaaggcata
    49981 atgaatattt ttgataatca agtcattacg aaccccagtc actttttggt gtctgagctg
    50041 attataatgt ttcaaatcgg tatagataaa gtctacgtag tcaataaggg tgacaaattg
    50101 ctcgtgtttg gcaaaggcgg tggtttcaat tgcggtgtga agacctgctg ctttggctgc
    50161 ttttaacagg gctaaggcaa agtcaaattg ggcaaagatt tcaccccctg acagggtcat
    50221 gcccccacct gactcttcgt agaaatcaag gtctttaagg acttcttcga tcacctcgtc
    50281 cacggttttt tcctctccaa ctattttagt attaagccca tcacttgtta acatctgctc
    50341 aggtgctttt tgttgggatt cgggattggc acaccaagga catctcaagg gacaaccttt
    50401 taaaaaaacg gttgtccgaa taccagggcc gtcatgaata ctaaaatgtt ggatgttgaa
    50461 aacaataccg cgatcagtca taagtgtgaa ttcctttcgt tgtaaaacca tcataccaga
    50521 taaacgtaaa taaatcaatt acgaatgaaa gtaaatatct cttttctttt aagaaaataa
    50581 ctggtacaat gaaagggggg tgaatgatga atcgattaga acgtattatt caactggttt
    50641 cccagaagaa aaaaatagat gttaacagtt tatcagagca attagaagtt tcgaaagtaa
    50701 ccattcgaaa agaccttgat aaacttgaaa gtaagggctt gttgcgacgg gaacacggct
    50761 atgcggtgct aaacagtggc gatgacctca atgtacgctt gtcctacaat tacaacataa
    50821 agcgacggat tgcagaaaaa gcagctgaat tggtgcaaga caacgatacc attatgattg
    50881 aatctggctc aacctgtgcc cttttggcag aagtgctttg ccagaccaaa cgaaatatta
    50941 aaatcattac caattcgtgc ttcattgcca actacattcg tcaatatagt agctgtcaaa
    51001 tcattttact gggcggttat taccagccca attcagaggt gactgttggt cctttattga
    51061 aagaaatgat atcccttttt catgtcaatc gtgtctttgt tggaacagac ggtttcaaca
    51121 aagatcttgg ctttatgggc aaggacatga tgcgctcaga aggggtgcgt tacatggcag
    51181 atgctgctga ggaagtggtg attttgaccg attccagcaa gttttcaaaa accagtctgg
    51241 tacaccagtt gtcattagct gatgtcaatc gcgtcattac agaccaagca ctagacaagc
    51301 aaacacaaga gttattaagt tctggtggtc ttgttcttga ttttgtttct tagaaaatag
    51361 gtggctagat gaaagaagaa cgcagacggc tccttgccaa ggtggcttac ttgcattatg
    51421 ttcaaggcaa gagccaaacc ttgatttcaa aggaaatgaa tatctaccga acaacggttt
    51481 gtcggatgtt agcaaaagcc aaggaagaag gcattgttcg gattgaaatt gccgactatg
    51541 atgctgattt atttgcttta gaagaatacg tgcgaaagca gtacggcttg gaaaaactag
    51601 accttgtgcc caaccaggtt gaagataccc caatggatac cttgacaaat gttgctaaaa
    51661 cagcagctga ggtttttaga catgttgtta aagatggcga taagatcggt ctgtcctggg
    51721 gagcgactct ttcttgcttg atggatgagt taaatccaaa agcgatgaaa gatgtgttta
    51781 tttatccttt ggcaggagga cctagccata ttaatgccaa ataccatgtc aataccctgg
    51841 tttaccgctt ggctcgcatc tttcatggta atagcgcttt tatgaacgct atggtcattc
    51901 aagaagacaa acacttggct aagggaatct tacaatctaa atactttaac gatattttaa
    51961 caagctggga ccagttagat cttgccttag taggaattgg tggggagcct aatagccttg
    52021 agcagagtca atggcgtgat ttattaacca gctcagatca tgaccaactc aaatatgaaa
    52081 aagccgttgg tgaagtttgt tgtcgtttct ttgatcaggc aggtcagcca gtttacactg
    52141 gcctacagga tcgaactatt ggtatttcgc tagaacagtt gaggcgtgtc cctaaaacaa
    52201 tggctgttgc aacaggtaaa cacaaggcta aggcaatatt ggcagcctta aaagcaggtt
    52261 ttatcaatta tctagtgacg gataaagaga ccatgctagc tgttttagcg ttagatgaag
    52321 atattgatct taataacgtt cttctttaat agaaattcaa agatactatg atatttatag
    52381 aggcccaaga ccaaacggtc agggcttttt tgtttgatgt gttgataaaa cagagattgc
    52441 tcaaatgtgc atttaatgta ttggcacaaa agtgcattaa acaaaatata gcacaaaata
    52501 tattgaccct aaaaacaaaa atgatacaat aaacttacca acgaaatgta aacgattaca
    52561 aacataataa catcaccttc gaaaaaaaag actcattgtc gatacatcac atgaaaaaga
    52621 aaaaaaggag acaccaatgc aagttattgt accagaccaa atcattatgg gcttaatttt
    52681 aaatgcaggc gatgccaaac aacatatcta ccaagctctg aaatgtgcta aagaggacga
    52741 ttatgccact agtgaaaaag aaatggcctt agcagatgat gccttattag aagcccacaa
    52801 tttacagacc cagtttcttg cccaagaagc tagtggtaat aagtcagaga ttacagctct
    52861 ttttgttcac tcacaggatc acttgatgac gaccattaca gaaattaatc tcatcaaaga
    52921 gattattgac cttcgtaaag aattggcaac taaataagtt ttcatgacta aaaaggagat
    52981 tgacatgatt aaaattggat tgttttgtgc agcaggattt tcaaccggca tgttggtgaa
    53041 caacatgaaa gtagctgctg aaaaaaaagg cattgactgt cagattgaag cctatgctca
    53101 agggaaatta gcggactatg caccattact tgatgtggca cttttaggcc cacaggttgc
    53161 ctatactctg gataaatcag aagctatttg taaagacaat gatatcccta ttgcagttat
    53221 cccaatggct gattatggaa tgttagacgg caacaaggtg cttgatttag cccttagcct
    53281 tgttaaagaa taggagtcaa tcatggcaaa aatgaatatg caaaacatca tcaagccaat
    53341 catgacattt gtgaatatgc gcggcatcat tgccttaaaa gacggtatgt tagccatttt
    53401 acctttgacc gttgtgggga gtcttttcct gattgctggg cagattcctt tccaaggggt
    53461 taatgatgcc attgctagtg tgtttggtgc tgactggaca gaacccttta tgcaggttta
    53521 ccatggaaca tttgctatta tggggttgat ttcctgtttt gcaattggct attcttatgc
    53581 taaaaactca gatgttgaac ctctcccttc aggggttcta tccttgtcag ccttctttat
    53641 tttattgaga tcatcttatg ttccagcaga aggtgaagct attggagatg ctatcagtaa
    53701 ggtttggttt ggtggtcaag ggattattgg tgctattgtg attggtttaa cggtcggagc
    53761 tgtttacaca acatttatcc gtcgtcatat cgtcattaaa atgccagatc aagtgccaca
    53821 agccatcgcc aaacagtttg aagccatgat tccagccttt gtgatcttta ccttgtcaat
    53881 gcttgtgtat attattgcca agtcagtaac aggtggtggc acctttattg aaatgattta
    53941 tgatgtcatt caggtaccgc tgcaagggtt aacgggttct ctttatggcg cactaggtat
    54001 tgccttcttt atttcctttc tttggtggtt tggcgtgcat gggcaatctg tggtcaatgg
    54061 aattgtcact gctcttctct tatcaaatct agatgccaat aaggccttga tggcagcagg
    54121 tgagttatcc ctagacaagg gtgcccatat tgtaacccaa caatttttag actctttctt
    54181 gattctatca ggttctggca ttacctttgg tttagtggta gctatgatct ttgcggctaa
    54241 atccaaacaa tacaaggctt taggtaaggt tgcagccttc ccagccctct ttaatgttaa
    54301 tgaaccagtt gtctttggtt ttccaattgt gatgaatcca gtcatgttct tgccatttat
    54361 cttggttcct gtcttggcag ctctcacagt ttatggcgcc attgctattg gtttcatgca
    54421 gccctttgca ggagtgaccc ttccgtggtc aacaccagcc attatctcag ggttcatggt
    54481 tggtggctgg caaggagcta ttgtgcaaat tcttatactc atcatgtcaa cgttggtgta
    54541 cttcccattc tttaaaatcc aagataatat ggcttatcaa aatgaacaag ctagtgaaga
    54601 gtcatgattt agcgtccaca caaattcttg agaccatctc cagttagagg tggtttttta
    54661 gaaaaagaag aacgatttat caggcctata ttgcacaata gtgcaaaata aagtgactat
    54721 ctcttcttat ggttaaggat acgcaaaagt cgcaatatat gataaaatag ttacgaaaga
    54781 aacatttata atttcgtaag aaagaaggag accccgcatg attgaaacaa aaagccctta
    54841 ttttggacat ttaacagaca gaatggctca ctatcgtgaa gctgtgttag acaagaaacc
    54901 ttatattgat gctgagcgtg ctattttagc cactgaagcc tatcaaaaac accaaaataa
    54961 gccagcaaac ctcaaacgcg cttacatgct tcaaactatt ttggaaaaca tgaccatcta
    55021 tattgaggat gaaagcctca ttgctggtaa ccaagcctct tcgaacaagg atgcgccgat
    55081 tttcccagaa tacactctgg aatttgtcct caacgaactt gatctttttg aaaaacgaga
    55141 tggggatgtc ttctacatta ccgaagaaac caagcaacag ctccgagaca ttgccccatt
    55201 ctgggaaaac aataacttac gtgctcgctg cggggtcttg ctaccagaag aagtgcaagt
    55261 gtacatggaa acaggtttct ttggcatgga aggcaagatg aactctgggg atgcccattt
    55321 agcggttaat taccaaaaac tcttggagca cggcttaaag ggatttgaag aacgggcacg
    55381 agctgccaaa gctgcgcttg atttgaccat tcctgaaaac attgacaaat accattttta
    55441 cgactctgtt tttattgtca ttgatgcagt caaaacttat gccaaacgct atgccaagtt
    55501 ggctagagaa ctagcaaaaa cggctaaacc agaacgtcaa gcagaattgt tagacatcgc
    55561 gcgcatttgt gataaagtgc cttacgaacc agcgaaaacc tttgcagagg ctgtgcaatc
    55621 tgtctggttc attcagtgta ttttgcaaat tgaatcaaat gggcattccc tatcttatgg
    55681 ccgctttgac cagtacatgt atccttatgt caaagctgat ttggaggctg gccgtgaaac
    55741 agaagacaca attgttgagc gtttgaccaa cctctggatt aagaccttga ccattaataa
    55801 agtacggagc caagcccaca ccttctcatc agcagggagt ccactttacc aaaacgtgac
    55861 gatcggcggt caaacacgcg ataaaaaaga tgcggtcaac ccactgtctt accttgttct
    55921 aaggagtgtg gctcaaacca aattgccaca acctaacttg actgttcgtt accataaagg
    55981 cctagacaat acctttatga acgagtgtat cgaggtcatg aaacttggtt ttggtatgcc
    56041 agccatgaac aacgatgaaa tcatcattcc atctttcatc aaaaaaggtg tctcagaaga
    56101 agacgcctat gattactcag ccattggctg tgtggaaaca gcagttcctg ggaaatgggg
    56161 ctaccgttgc acggggatga gttacatcaa cttccctaaa attttgctga ttaccatgaa
    56221 tgacgggatt gacccagcat caggcaagcg ctttgcaaaa ggacacggtc acttcaaaga
    56281 catgacatct tatgaagaat taaaagcagc atgggatgcc accctgcgcg aaattacccg
    56341 catgagcgtc attgttgaaa atgccatcga cctaggtctt gaacgtgaag taccagatat
    56401 tctttgctca gccttgacag atgattgtat cggtcgtggc aagaccttaa aagaaggcgg
    56461 agctgtttac gactatatct caggccttca agtcggtatt gccaacctat cagactccct
    56521 agcagcgctg aaaaaattag tatttgaaga aggtcgatta actccagaag aactctggca
    56581 cgcccttgaa agtgactttg ctggagagcg cggagaagag atccgacaaa tgctcatcaa
    56641 tgacgcacca aaatatggta atgatgatga ctacgcagat agtttagttg tcgaagctta
    56701 cgacacctac attgatgaaa ttgccaaata cccaaatacc cggtatggcc gtggcccaat
    56761 cggaggtatc cgctattctg ggacatcatc aatctcagcc aatgtcggac aaggaaaagg
    56821 aaccttggcg acaccagacg gtcgccatgc aggcacacca ctagcagaag gctgttcacc
    56881 agaacacagc atggacaaaa aaggcccgac ttcagtattg aaatctgttg ctaaattacc
    56941 aacagatgag attgtcggag gagtgctact caaccaaaaa gttaacccac aaaccctggc
    57001 caaagaagaa gacaaactga aattaatggc cttacttcgt accttcttta atcgcctaca
    57061 cggttatcat atccagtaca atgtcgtgtc acgtgaaacc ttgattgatg cccaaaaaca
    57121 tcctgaaaag caccgtgacc tcattgtccg tgttgccggc tactcagctt tcttcaatgt
    57181 gctctcaaaa gcaacccaag atgacattat tgaacgtaca gaacacacac tttaggagaa
    57241 aataagtatg gaatacatgt tagacactct agatttagaa gctattaaaa aatggcatca
    57301 cattttgcct ctcgcaggtg tgacctcaaa cccttctatt gccaaaaaag aaggtgacat
    57361 tgatttcttt gaacggattc gagaagttcg tgccattatc ggtgacaagg cttctattca
    57421 tgttcaagtg atcgcccaag attacgaagg catcttaaaa gatgctgctg aaatccgcag
    57481 gcaatgtgga gatagtgtgt atgtcaaagt acctgtaacc acagaaggcc ttgcagccat
    57541 taaaaccttg aaagccgaag gctatcacat cacagcaacc gctatttata cgaccttcca
    57601 aggactatta gccattgaag ctggtgctga ttatttggct ccttactata accgtatgga
    57661 aaatctcaat attgatccag aagctgtcat aggacaatta gcagaagcta ttaaccgcga
    57721 gaatgctgat agtaagattt tggcagcaag ctttaaaaat gtggctcagg tcaataaatc
    57781 ctttgctctg ggagcacaag ccatcactgc tggcccagat gtgtttgaag caggatttgc
    57841 catgccatct attcaaaaag cggttgacga ttttggcaag gattgggaag ccattcatca
    57901 ccgcaagagc atttaagaaa tagcatcagt agtaatcaca tcattaggaa tcacagataa
    57961 aggagttcaa cgatgaaagt atttgcaagt ccatctcgtt acattcaagg taaaaatgcc
    58021 ttgtttacca atgctaaaac cctaaaacaa ctaggaaata gccccatttt actgtgtgat
    58081 gacgtggtgt atggcatcgt cggagaaaga tttgaaagct atttgataga taatggcatg
    58141 actcctgttc acgtagcctt taatggcgaa gcctcagata atgaaatcag ccgcgtggtt
    58201 gccattgcca aggaaaatgg caatgatgtt attattggac ttggtggtgg aaaaaccatt
    58261 gacagcgcta aggctatcgc tgacttactt gctgttccgg tgattattgc cccaaccatt
    58321 gcttcaacag atgccccaac ctcagcctta tcggttattt ataccgatga aggagccttt
    58381 gaaaaataca ttttctattc aaaaaatcca gatcttgttt tggttgacac acaagtaatt
    58441 tgtcaggcac caaaacgctt attggcgtca gggattgcag atgggttagc aacatgggta
    58501 gaggcgcgtg ctgttatgca aaaaaatgga gacaccatgg caggtggcaa tcaaaccttg
    58561 gcaggagttg ccattgccaa agcctgtgag cagaccttgt ttgcagatgg cctaaaagcg
    58621 atggctagct gtgacagaca agtcgtgacc ccggccttgg aaaatgtcat tgaagccaat
    58681 acacttctta gtgggcttgg ttttgaaagt gcgggtctgg ctgctgcaca tgccattcac
    58741 aatggcttta cagcgctgac aggtgctatt catcacctta cccatggtga aaaagtcgcc
    58801 tatggcacgt tgacccaact tttcttggaa aatcgttcgc gtgaagaaat tgaccgctac
    58861 atcgactttt accaagctat tggcatgcca acaaccctca aagaaatgca tttagacacg
    58921 gctactcaag aggatttctt aaaaattggc cgtcaagcaa ccatggcagg cgaaaccatc
    58981 catcaaatgc catttgttat cagtccagaa gatgtagcag cggctttggt tgctgttgat
    59041 gcctatgtta cgagtcgata ggctaaccaa aaagagattt cttatttgcc agataacctc
    59101 aaaaaaacaa gtctagggag tcatttccta gacttgtttt acttattgtt ggttggtaat
    59161 agcactttta gcagggtcat tgtgggcaat acggctggca gctgcagccg caatggcgct
    59221 aacaatatca tctaaaaagg tatggcagga gttgccatct ttgtggttga gtttgtcaac
    59281 aatccccggt tttgtcttgt ccagataccc ataattggta aaaccgatag acccatagag
    59341 gttaacaatg gataaggcca ggatttcatc aatcccatac agcccttggt cggttttcaa
    59401 gatactaagg agtggctcag acagctggtt ggcttcagcc aatttatcca attctacccc
    59461 agtgataata gcattttgca cttctcgttt tgcaagcacc gcttcaacgc tctctaaaca
    59521 ttctgccatg gtcaaattgg gaatataatc attctgcaaa aaaagcacca gctctgcaat
    59581 agcttctaaa gaaacccctc gttcagccag taattgataa gaaacttctc gtaagtgatg
    59641 gtcagttgac atgtgagacc tccttttttt ctttattgta acataaaaga ttttaagaaa
    59701 cctctacaac agctaaagag gggatagttc ttttttaaga gatgttgact actgagaccc
    59761 cctacttggt aaaagtcaaa gaaacgatag taaagtaacc atcttaccta ggcctaaata
    59821 caatcaccac ccctaggtga cacacagcac ctatttcaaa aatgatatta gtatggcaaa
    59881 acaaaaagac aacgccttct tttttctcct tattatctcg tttaattttc atatttttta
    59941 aaaaaactat tgataaacta gttaagtaag cgtatactat ggttagtgag cgaaattaga
    60001 aaagaggaca agcatatgaa tctacttgga tcaagacggg ttttttctaa aaaatgtcgg
    60061 ctagtgaaat tttcaatggt agctcttgta tcagccacaa tggccgtaac aacagtcaca
    60121 cttgaaaata ctgcactggc acgacaaaca caggtctcaa atgatgttgt tctaaatgat
    60181 ggcgcaagca agtacctaaa cgaagcatta gcttggacat tcaatgacag tcctaactat
    60241 tacaaaactt taggtactag tcagattact ccagcactct ttcctaaagc aggagatatt
    60301 ctctatagca aattagatga gttaggaagg acgcgtactg ctagaggtac attgacttat
    60361 gccaatgttg aaggtagcta cggtgttaga caatctttcg gtaaaaatca aaaccccgca
    60421 ggatggactg gaaaccctaa tcatgtcaaa tataaaattg aatggttaaa tggtctatct
    60481 tatgtcggag atttctggaa tagaagtcat ctcattgcag atagtctcgg tggagatgca
    60541 ctcagagtca atgccgttac aggaacacgt acccaaaatg taggaggtcg tgaccaaaaa
    60601 ggcggcatgc gctataccga acaaagagct caagaatggt tagaagcaaa tcgtgatggc
    60661 tatctttatt atgaagccgc tccaatctac aacgcagacg agttgattcc aagagctgtc
    60721 gtggtatcaa tgcaatcttc tgataatacc atcaacgaga aagtattagt ttacaacaca
    60781 gctaatggct acaccattaa ctaccataac ggtacaccta ctcaaaaata ataccaaaag
    60841 gctagacctc tgctcactag gcctagcttt ttacatcaaa aaaagcaatg actatagaaa
    60901 gtaaaaatac tagaaaaagc aatgattgcc gtcattgctt tttatgattt gtcaaaaagt
    60961 aaaaagcaaa ttgaaaaaat cgccctggag ctgttgagat aaactacacc atttattaag
    61021 ataccaaatc aaaaagctaa caccataaga gcgttagctt tttaaagagc ctcaggacag
    61081 tttatgttta atggcttcta ggtaggtttg aaacatatga tggatcgttt tgcaattgag
    61141 taattggaag atctctaaga tctgcacaca cttttgctca gccccctctt taccatttat
    61201 gaggccaata atgccatcaa aaaaagacac acaacaccgt tcatataagc aatcatcatt
    61261 tagctgataa tcttcaattt ttgccaaaat ctcagaagct ttatcatact cttgtcgctg
    61321 aataaacaaa atcaacatat tgacaaacat ccgaatcgat tcattcccat aataccttag
    61381 ggtattgtat ttatcgaggt tcaaaataac ttttgaaaac accatctcaa taaaacaaga
    61441 ctcaaaaata aacatacaat tattaaaaag cacagtctca tagtgactcc aagtttcaat
    61501 attaataaga tagtttgaca aataagtccg ctctgtcaga cagtcttcct cagtaagagt
    61561 tgctaataac accttgacca aggcaaaaag gttcttttcc tttgttgact tactatcttt
    61621 ataagaatca aggatatgat ttaaaccttc tatgtttcgg cattcaaaat aatgttttgc
    61681 cgtatccata tcaataaatt ctttatattg tttaaagtta ttactaataa agagaaactc
    61741 atcaacattg acattcaagt tgtctaagat ataaagtaac tttttcgaag aaatgtcaag
    61801 attattttta ataaaacgat aataggtttg cctagtgaga taatcaccac aaacttgttt
    61861 aatcgaaatg ttttttgaat gcctaatgaa ttcaacggtt tcaccaattt ccatatgtca
    61921 agccttccta gttgatgtca aaaatacgtt acgcatgtac ctaatacgta acaagttgaa
    61981 tgtttcggat gatagtcgct tatgataggt gcataaggtc aatagccaga tgcgatagtt
    62041 ttatcaactg tcatatgtta aacctttcta atctaataga tgttaaaaat acgttacgtg
    62101 tgtacctaat acgtaacaaa ataatgggtt agcaaaataa gcagctatga tatagccata
    62161 aggttaaaag gaggcgccta ctatgtggtt attgttacta tttttgtagt tccttttgca
    62221 aaatggtagt agataaaaat gagaagtcaa taaaagcatt gacaaacaaa agaatcattt
    62281 tgtttatatt atcagtattt ttaagtttct tgtcaactga aatgagcatc tactagccac
    62341 aatagtaact aaaaggctaa ttgtcagtaa aaatgagata aatcagccag gaaaaagcct
    62401 acgagtcaac taatggacaa ccccaaaaaa gacaaaggac tttcacttgg tgttgtggac
    62461 cctatcacga tccttatcac agaatcagtt attaacaaaa tgagaacggt cccatatcat
    62521 ctaaaaaaac tgtcaagcac aacctattgt ttgctagctg atcttgccta tcagtctgcc
    62581 tagtagatac ggcagaagat aagagatcaa ccaaaagatg ataagtgcaa cgactcatag
    62641 cgtcttttga ccaaaaccaa ttagactcac atgagatgag aataattggg ttgggttgtc
    62701 agtgtcaact aaccgtgtta ttgtctatta ccattcatgg tatcagcgac atcgtatgat
    62761 aaccatgcga ttcagctaag taaggaggtg tgtccaatgt actgttaaaa gcaaatgcag
    62821 tagattaact tattttgaaa gaggtataaa aaaaatgaat aaaaagaaat taggtatcag
    62881 attattaagt cttttagcat taggtggatt tgttcttgct aacccagtat ttgccgatca
    62941 aaactttgct cgtaacgaaa aagaagcaaa agatagcgct atcacattta tccaaaaatc
    63001 agcagctatc aaagcaggtg cacgaagcgc agaagatatt aagcttgaca aagttaactt
    63061 aggtggagaa ctttctggct ctaatatgta tgtttacaat atttctactg gaggatttgt
    63121 tatcgtttca ggagataaac gttctccaga aattctagga tactctacca gcggatcatt
    63181 tgacgctaac ggtaaagaaa acattgcttc cttcatggaa agttatgtcg aacaaatcaa
    63241 agaaaataaa aaattagaca ctacttatgc tggtaccgct gagattaaac aaccagttgt
    63301 taaatctctc cttgattcaa aaggcattca ttacaatcaa ggtaaccctt acaacctatt
    63361 gacacctgtt attgaaaaag taaaaccagg tgaacaatct tttgtaggtc aacatgcagc
    63421 tacaggatgt gttgctactg caactgctca aattatgaaa tatcataatt accctaacaa
    63481 agggttgaaa gactacactt acacactaag ctcaaataac ccatatttca accatcctaa
    63541 gaacttgttt gcagctatct ctactagaca atacaactgg aacaacatcc tacctactta
    63601 tagcggaaga gaatctaacg ttcaaaaaat ggcgatttca gaattgatgg ctgatgttgg
    63661 tatttcagta gacatggatt atggtccatc tagtggttct gtaggtagct ctcgtgttca
    63721 aagagccttg aaagaaaact ttggctacaa ccaatctgtt caccaaatca accgtagtga
    63781 ctttagcaaa caagattggg aatcacaaat tgacaaagaa ttatctcaaa accaaccagt
    63841 atactaccaa ggtgtcggta aagtaggcgg acatgccttt gttatcgatg gtgctgacgg
    63901 acgtaacttc taccatgtta actggggttg gggtggagtc tctgacggct tcttccgtct
    63961 tgacgcacta aacccttcag ctcttggtac tggtggcggc gcaggcggct tcaacggtta
    64021 ccaaagtgct gttgtaggca tcaaacctta gtatggaaat gcatttcgtt agaacagaac
    64081 ctgaggcacg ccgcatagct gaaacctttt gtgccgaaaa cacacaaaca aaaaccccta
    64141 tgcgcgtgca gcagttaagc tatccatcag acacagatca ctcaggtggt gagctataca
    64201 tctatgcttt gtctcctgct ggatttatca tcgtatcagg agacaccaga gcgcacacca
    64261 ttttaggcta ttcttttgat aataacctag acctcaacca tgataatgtc aggagtatga
    64321 tagaagctta ccaaacacaa atcaactctt tggattgatc ggtcaaagta gcttttgccc
    64381 ccctcaccac tcgtttaaga cgaccgtgtt tctaaaatac cactaccttt taagtttcac
    64441 gccggcctaa gcgagtgttt gtctcatgga gcagactgtt tccatcgaaa agtcacccat
    64501 tgataaagga ggtcatcaac gcctactaat gatgacttcc ctaagatgcc aaggtaaagg
    64561 actgagttct gtagagatgg gactcagtcc ttttacgatt tttgggagtt attctagcca
    64621 taccgtggca agaaaacaac acttaaaaat agccaacgtc agtttctgtt catacatgac
    64681 tacagccaaa caccacaacc taacctatcc taaacacagt ggctttctaa cgaacagtca
    64741 caacgagcag caacaccaaa aagcaagatt aagctacgtt taagactaat gatataagat
    64801 aacagtaatt attaatgaga aaagaggctc aggacatgaa acaaatgaat aaactcatta
    64861 caggagtggt aacgctggcg acagtcgtga ccttatcagc ttgtcaatca tcacacaaca
    64921 acaccaaact cgtctcgatg aaaggagaca ccatcactgt cagtgacttc tacaatgaga
    64981 ccaaaaacac agaactcgca caaaaagcca tgttaagctt ggtgattagc cgtgtatttg
    65041 agacacaata tgccaacaaa gtctctgaca aagaggttga aaaagcctat aaacaaaccg
    65101 cagaccaata cggtacatcc tttaagacag tcctagcaca atcaggctta acgccagaaa
    65161 cctataaaaa acaaattcgc ctcacaaaat tagtcgaata tgccgtcaaa gaacaagcca
    65221 aaaacgaaac catctcaaaa aaagactacc gtcaggccta tgacgcttat accccaacca
    65281 tgaccgcaga aatcatgcag tttgaaaaag aagaggatgc caaagcagcc cttgaagccg
    65341 tcaaagctga aggggcagac tttgcagcta ttgccaaaga aaaaaccatt gcagccgata
    65401 aaaaaacaac ctacacgttt gactcaggtg aaacaaccct accagcagaa gtagttagag
    65461 ctgcatcagg cctcaaagaa gggaacagat cagaaatcat cacagcgctt gatccagcca
    65521 cctcaaaacg cacctaccat atcatcaaag tcaccaaaaa agcaactaaa aaagcagact
    65581 ggaaagcgta ccaaaaacgc ttgaaagaca tcatcgtgac tggcaaatta aaagaccctg
    65641 acttccaaaa caaagtcatc gctaaagctc ttgataaagc aaatgtcaaa atcaaagaca
    65701 aagcatttgc caatatctta gcccagtttg caaaacctaa ccaaaaacaa cctgcccaaa
    65761 aatagagggt tgccagttta tttccctaag gttgcttaaa aagacagccc taaccatcac
    65821 tccccttttc aaaacaccga taatggaaac gtgtcctata ctgatggtct agggcaatgt
    65881 cataaagcta aagactctat ccaaataagg atagagtctt taatatttaa aaaccagtca
    65941 gcaaaaaaaa ttcacgctaa gcctagctag aatgaggctt agcgtgaatg gtatcagtga
    66001 tagtccgtta acatagctga ttgggagtca gaaactctaa ctacccaatc tgactatacc
    66061 cccagtacca gaggtgtctc atcgcagaag tagcaatcag ataatcgtct gataagctct
    66121 tgtcctcaag caactgatga atatcgtcgc acgggccatg agctgccggt ggctgatagc
    66181 tagtaggctc atcagccaaa acttttttgg agtaacgctt tggtctcaga cggcgccaga
    66241 gggttgagag aggggtgaga aaaaaacgat gggtcatctt ggcctccttg tctaatgata
    66301 gtcaccttaa cagacaaaag tgtcaataat gtcattttaa ggagaaaaag attaaaaatc
    66361 aaggtcacaa actttccttt gttaaaaaaa cgcgacccta acaaagaatc taaatacctc
    66421 tcgcacttga gcttctcctt gcgcagctat cttgtgcact cagttgcctc caagataatg
    66481 ataacgatac catcattatc ctataggcta tcgcgacaac cccttttttc gcctcaacga
    66541 gtctgacaca gctgtcatga cagaaaaaag acctctgcct atctaagaga ccattaattt
    66601 ttctctcact aaaaaactga ttttagaaac gaagaagcat ggtgtataat aaggttcaga
    66661 acaattatga cataataatg aaagtaaggt taacgaaaca ttgacaaatt gtaagtataa
    66721 acttagaaag ttatctgtag ggctcgtctc cgtcggaacg atgctgatag ccccgacagt
    66781 tttaggacag gaggttagtg ctagtagtag tacggagagc agtaccacta cagctaatac
    66841 tggtaccggt acggcaagtg ggatgactgc cactactcct agtgctacga cagatactgg
    66901 tgaagcagct gggagcggag ctaggagtga agctaatggt gcatcgtccg tagtatctag
    66961 cgaagaaagt cagagttcag gcactactcc agcctcaccc caagcacaga cagctccagc
    67021 agcaacgtca acatcatcgg cttcttctag taatgagaaa actcccaaga cagcaactac
    67081 aactacatca tcgactccag tagcaagtac cagtaataat agcaacaaag taactagtac
    67141 tgaagctgaa acacagacga tggacgtgga acggtataca gttgataagg aaaattcaaa
    67201 gctaaatatt aaagacggta agactccaaa aactaggagt agtgttaata aagacacaaa
    67261 acttattaga aaccgcgatg acaaacagcg tgatatcgtt gatgttactc ggacagttaa
    67321 aactaacgaa gatggcacta ttgatgttac cgtaacggtt aaaccgaagc aaattgacga
    67381 aggcgccgat gttatggccc ttctagatgt ctctcaaaag atgacaaaag agaattttga
    67441 taaggctaaa gaacaaataa aaaaaatggt tacgacttta acaggcgagc caactgatgg
    67501 taaggaaaat cataataggc gtaattctgt acgtctaatg actttttacc gtaaggttaa
    67561 tgagcctatc gaattgactg ctgaaaatgt agacaaaaca ttagatgaag tttggaaaaa
    67621 agctaaagaa gattgggact ggggcgttga cttacaggga gctatccata aagctcgaga
    67681 gatttttaat aaggaaaaag aaaaaaaatc gggtaaacgc cagcatatcg tcttgttctc
    67741 tcaaggcgaa tcaaccttta gttatgatat taaaaataaa agtgatatta caaaaacaaa
    67801 aataactgaa aaagttacga cctctagccc actgtttcct tggctcccta tctttaatca
    67861 cactaaccga aaggcagaga ttattggtga tttagaaaaa gtactagaca tggccgaaaa
    67921 agtgggaata agtttaccta gtagtctgaa gttagcagtg aaagcccttg gcttaactaa
    67981 tagtgcaata ggttctattt tagggaaagg tttgacagag taccttggtt tgacagaata
    68041 tagttcagat aacttagatg gaggagggtt tgattatagt aaacgtgtag gggaaggtta
    68101 ctactaccac agtttttcag ataggaaata tgaaaataca atgccccttg aagaagctat
    68161 caggacggcc ttagcatcta attttcccaa actcacagat aattggtttt tcgatatctt
    68221 aaatagtttt gtcaataaag atacagttga gaaagctaaa ttagacgtaa ttatgaaggt
    68281 acttaatagt attttttaca aacgtgaata tcgctattac aaccataacc tgtcagcaat
    68341 agccgaagct aaaatggctc aacaagaggg cattaccttc tattccgttg atgttactga
    68401 tttaaactca gcttctaaaa gagtgaggcg acaagcagca gtgcgtaagg gcactaagga
    68461 agaagaaaac aaaaaaaatg aagaagagcg taatacaaag tttgacacct atctgaaaaa
    68521 gatgtctgaa ggtaataatt tcttaagtaa tgttgaagag agagataagt tcaaagatac
    68581 tttaacagag ctgacaatta aagacgaatt tacggataaa gttacggtcg agaaggattc
    68641 ctggagtaaa tctataactg atggacttaa aaattcaaat aacaataatg ttaaacatca
    68701 acaggcaaac acatcgacat ggagtttctt cagttcatcc aaagaaagcc tcacctggat
    68761 tatttccaaa gagcagctca aagaagcctt tgagaaaaat ggttctctca cttttaaata
    68821 caaattacgg gtcaataaag acaaactatt agataaagat aagaagagaa caaaacgtga
    68881 tacacctacg gaaaataaga cttctgtaac ggcagatatc atttcaaata ctgttgacta
    68941 caaaattaat aatcaagaag ttaagggtaa caaacttgat gctgtcaagt tgacttatac
    69001 taaagagacc gttcctgttc cagatgtgga aggagaagtt gtaccaatac cagaaaaacc
    69061 actggtagaa ccaatgacgc ctctataccc tgcaattcct aattacccta cccctcaact
    69121 tccaaaagat gaagatctgg agattagtgg aggtcatgga ccgattgtcg atatcgtcga
    69181 agatactggt acaggtgttg agggcggcgc tcaaaatggt gtggtttcaa ctcaggaaaa
    69241 taaagatcca atcgttgaca tcaccgaaga tacccaacca ggtatgtcag gctcaaatga
    69301 cgcgacagtt gtcgaggaag acacagcacc taaacgtcca gatgtccttg ttggtggtca
    69361 aagtgatcca atcgatatca ccgaagatac ccaaccaagt gtgtcaggct caaatgacgc
    69421 gacagttgtc gaggaagaca cagtacctaa acgtccagat atccttgttg gcggtcaaag
    69481 tgatccaatc gatatcaccg aagataccca accaggcatg tcaggttcta atgacgctac
    69541 tgttatcgaa gaagatacga aaccaaaacg cttcttccac tttgataacg agccacaagc
    69601 accagaaaaa cctaaagagc aaccatctct cagcttacca caagctccag tctataaggc
    69661 agctcatcac ttgcctgcat ctggagacaa acgtgaagca tcctttacaa ttgttgctct
    69721 aacaattatt ggagctgcag gtttgctcag caaaaaacgt cgcgacaccg aagaaaacta
    69781 accctcgtta gtctagcgac cctaagcctt tggctttaag aatttctcat tatccccctc
    69841 aaacaccggt acctcactga gatggcgctt taaaaaggcg ctaatctcaa atcaggtatc
    69901 ccctacggtt gagggggatt aatgggtatc acaacaagaa aaaaggtata atgatgaaat
    69961 atgactaaaa aatttattaa atcagcagtg attctaggct tagcaagcat aaccatcagc
    70021 atgacgcaac ccgttgaagc catttcaaat agcttagaat atcaagcaca atacgaacgt
    70081 gtcaaagaag aactagacgc attaaaagaa aagggcccaa aattaggaga acgttccctt
    70141 tattttagat tggtgaataa atttctaagt tggtttggcc aagtctggga cccactcgca
    70201 atcaaagaaa ccgattacct gttggacctc atcggacaag tcaattactt ggtgagtaac
    70261 accgagtttc aagcccctga gtacaaagat gctaaaaaca aactagactt agctgtagca
    70321 ggctatgacc tctacctagc tagagagaaa aaacgtggtg atgctcctga ttaccaagtg
    70381 acccagctaa cgctagaaca gctcaaaacc atcaaaaaga agatacgaga ggccctcgaa
    70441 acatttgcac aagaggtcaa aacaatccag caaaaaaacc cagacctatt tccccatgct
    70501 tatgttttaa acgctaagag caaagatatc acagataaag cgcaaccctt tttagtaaat
    70561 agttactacg atgtctttta taagcagttt ttattcacta atacaaatga cgggtacaac
    70621 tataataaag aagcagacaa tctgacctcg tatcaaaatc tgattaaaga gctattaggc
    70681 ttaaaagctg agatcgatca gttaacaaaa ggcaagactg gcgatgaccg tttagagatg
    70741 ttaatgtggc aatcaaagtt caccttatct gattcacgga aaaaatcaca ccttagcgcc
    70801 tactatacac aaggcgagta tgtcgataag gtggtcctat ctgaagaagc tcccaacttg
    70861 agaacagcaa aagaaaaggt caagacctta aaagggatct tgcatgatta ctatgttgaa
    70921 actaataagc cagaaaaagc taaaaagtat catgtcgagg acgagacctt gccgtctcaa
    70981 cctgaggctc ctgctacacc tgaagctcct gctcctgttc ctgctcctgt tcctgttcct
    71041 ggtcaaaaac cagcagaaga tgagagatcg ccacaagcta cggagccagc taacccgtca
    71101 aaagaggatt cctcaacgga cgcatctcag ggatctcacg atagtgaaaa cccagcgaca
    71161 gacagtcctt ctcaacctca ggcaccagac caaggcaatc accaaagtca agtgcctaac
    71221 gccaagcctc aaacggacaa gacggacact cctaacgccc cagccatccc acaggacacc
    71281 ccaaaagcgc ctgaggctgg tggtcagagt ggccccgctg ggaacactga ggagaaagct
    71341 cctgactctg gacctaaaga gtctgatcaa agctcatcta aagagtcccc atccgttggc
    71401 gaagacacga cccctaatca gccagatgtc cttgtaggtg gtcaaagtga gccaattgat
    71461 atcaccgaag acacaatcac tgacgctcct ccgacagtat ctgggcacaa cgcatcaaca
    71521 cagccccaat ctgtggtcga agacacagca cctcagcgcc cagatgtcct tgtaggtggt
    71581 caaagcgagc caatcgatat cacccaagat acccaaccgg gcatgtcagg ctctaatgac
    71641 gctaccgtta tcaatgaaga tacaaaacca aaacgcgtct tccactttga caacaaagag
    71701 ccacaagcat cagaaaaagc ggcagagcaa aaacttgctc ctcatgatag ccacacaacc
    71761 cctcaagctt cagatgatac agcagcccct cacttgcctg catctggaga taaacctgaa
    71821 aaagtgtttg cacttgctgc cttgacaatg gtcgcagctg cagggctact cagcaaaaaa
    71881 cgtcgcaaca ccgacaaaaa ctagtcgctg ttaggcacaa cgcatcacta aggccagtgc
    71941 atttgcactg gccttagttg ggtttttatc aaatagaggg tttttcatga caattgttag
    72001 cacttcggtg taaaataaaa gtaataacca gcaaaggagc aacctatgac aaagcaagat
    72061 cagttaatcg ttgaaaaaat ggaacaaacc tatgaggcct ttagcccaaa gctagcaaat
    72121 ctcatagaag ccctagatgc ctttaaagag cactatgagg aatatgccac acttagaaat
    72181 ttttacagta gtgacgaatg gtttcggctt gcaaaccaac cttgggatga tatcccgtgc
    72241 ggtgtcctat ctgaagacct cctctttgat atgatcggtg accataacca actactagct
    72301 gatgtcttag acttggcccc tatcatgtac aagcatatgt aaccattgtt aacgtcacag
    72361 atactacgat gacaggacaa aaaaagcttg aaaatctcaa aaaaaaacag ataaaaaaca
    72421 gataatcgtt agaaaaacag tagatgcgtc tgttaaacta aaggtatcaa aacaaaagga
    72481 gcatttcatc atgaaattca aaaaagtatt agtcattcct gcattagcat tagcagcaac
    72541 atgcttttta acagcttgtg gcacaaaaaa agattctaaa aaagaagaag ttaaagaaat
    72601 caaaatgtct gacatcaaag acgacgcagt tagcaaaaaa acaaaagtgg ttgatggcga
    72661 agaagttaca gaatacacca caaaagatgg aaacgtgatt cagattcctg caggaaacga
    72721 agaaggcatg gaatcaaaag atgctggtgg atcaggcgct cctgctaaaa actaagggtt
    72781 acaacaaaga gcataaggtg actcgcaaac gttacgcatg acatgaggca gtctgcctaa
    72841 gatggtaact cacttatcat aatgcctaaa tagccaaaca cactaactcg caaatgtaca
    72901 aaatcatata cccgtgctta tgtgagcgaa cggttatatg atttttgttt tccaaaaaca
    72961 agaaaagaac gctaaaatag ccagtttgtc gtgtttcaag ctaccaaaaa ggaagtgcta
    73021 agaagtgcta aaatgaaaaa aagatccatt atacctgtca tcttactgtt ggtcatcgga
    73081 ttaatcgttg gtgttaagcg tcatcatgac tagtaggtct tgctagtgac cgttcaaaag
    73141 ggataacgtt ggtatggtat aaccataaaa atcgtataaa aagaaaaaat agctattgag
    73201 gagaaagtat gtttcagtta agaaaaaaaa tgacgcgcaa acaattagcc ttgttgagtg
    73261 ctggagtgtt gacctgtgtg gttggtggta gctacttgat aatgaaccat caacaacaag
    73321 aaattgtctc tagtgtcaac aaagtaaaag ccttaaccat aaaagaagcc atggaacaag
    73381 gaaaagatat cagcttgacc ttagctggcg aagtaacagc taacaacagc agcaaagtca
    73441 aaatcgactc aagtaaagga gaagtcaaag atgtctttgt caaaaaaggc gatgttgtca
    73501 aagtagggca acccttgttt agttatgaaa cgtcacaacg gttaacggct caaagttcag
    73561 aatttgatgt tcaaaccaaa gccaatcaac tccaagttgc taaaaccaat gcagcattga
    73621 agtgggaaac ctacaatcgc aaggtcaatg aaatcaatac cctaaaatct cgctacaaca
    73681 ctgcaccaga tgagagctta ctagagcaaa ttcgcagcgc agaagacagt gtatcccaag
    73741 cactaagcga tgccaaaaca gcagatagcg atgtcaaaac cgctcaaatc gaactcgata
    73801 aagctaatgc tactgccaca acggaaaaag gtaaactaga gtatgacact gttaagtcag
    73861 acactgcagg aaccattgtt agcctaaata ctgatttgcc aaatcaatca aaatccaaaa
    73921 aagaaaatga aacttttatg gaaattatcg acaaatcaaa aatgttagtc aaaggtaaca
    73981 tcagtgaatt tgaccgtgac aagttaaaaa tcggtcaaaa agtcgaagtg attgaccgca
    74041 aagacaactc taaaaaatgg actggaaaag taacccaagt tggcaacctc aaagcagagg
    74101 aaaaaggcca aggtcaaggc caaggtggca atgaccaaca agacaatcca aaccaagcaa
    74161 aattccctta tgttatcgaa cttgaccaat cagacaagca gccactcatt ggttcacaca
    74221 cctatgttaa tgtgctcaac aatgttccag aagctggcaa gatcgtattg aaagaaacct
    74281 ttacaatggc agaaaatgga aaaacctatg tgtggaaagt tgataaaaac aaggtcaaaa
    74341 aacaagaaat caagactaag cccttctcaa aaggttatgt tgaggtgaca agtggcttga
    74401 ctatgcaaga taagattgct cagccgcttc ctggcatgaa agacggtatg gaggtaggaa
    74461 gtattgttaa accttaaaga tattcgaaaa agctatcatc ttgggactga agaatttgcg
    74521 attttaaaag gaatcgattt agaagttaac gagggtgact ttttagccat catgggacca
    74581 tcaggttcgg gaaagtcaac attgatgaat atcattgggt gtttagataa gcctggctct
    74641 ggctcatatg ccattgaagg cagagacgtg tcatccttat ctgataatga acttgctgat
    74701 ttgcgtaatc aaaaaatcgg ctttgttttt caaaacttta acctgatgcc caagctaaca
    74761 gcttgtcaaa atgtcgaatt gcccttgact tatatgaatg ttcctaaaaa agagcgtcgc
    74821 aaacgagcgc tagagatgtt aaagctagta ggactagaag aacgcagtga atttaaaccg
    74881 atggagctat ctggtgggca aaaacagcgt gtagcgattg caagagcttt agtcactaac
    74941 ccgagtttta tccttggtga tgagccaaca ggtgcactag acacaaaaac cagcgtccaa
    75001 atcatggacc tatttaaaca attcaatgat aacggcaaaa cgattattat catcacacac
    75061 gagcctgaag tagctgcctt atgcaaaaag acggtgatcc taagagatgg taatatagaa
    75121 cattccgata tagagtaaag ggggaaaata atggaagata ttattgcagc tttaagctct
    75181 atattatcac acaagatgcg gtcaattttg acaatgctag ggattattat cggtattggt
    75241 gccattatcg caatcttttc aatcatcgaa ggtaataccg aaaataccaa aagacagttg
    75301 attggtggca gcaataacac tattaacatt gtgtttaaca aaaaaagcag tattgaccct
    75361 aaatttccag acaagtcaaa tgctaaaaaa ccagactatc ttccttttat ggctgaagaa
    75421 gagcttagca aaatacagca agtcaaaggt gtcaaaaacg ccctgatctc atatggtatt
    75481 gatgacaaag tctaccatct aggccaaaaa tcatctgcaa agatttctgc catcacaaaa
    75541 aacgtagctg aagtgaggcg catgacattt attaaaggca gtgacttttc agacaaagac
    75601 tttattgacc aaaaacaggt gatttacctt gaaaaaagct tgtatgagtc actatttcca
    75661 aaagacgatg gccttggaaa atttgtcgaa gtgatgggaa atcccttcag ggtcattggg
    75721 gtgtttgaat caaaagaaca aagtggcctc acaagtggga ctgagaaaat tgcttatatc
    75781 ccgctacatc agtggtataa tattaatggt gtagtggatg cgacacctga gatcacgatc
    75841 cagacctatc gcgcagatga cctcaaacct gtagcaaagc gtgtcagtga tatgctcaat
    75901 caaacgatcc ctaaatcaga ttatatgttt ggtgtcatga accttaaaga atttgagcgc
    75961 caactagaca acctaaacaa atctaatttt gtgttattag ctggtattgc tagcatctct
    76021 cttattgtag gtggtattgg tgtgatgaac attatgctag tatcagttac cgaaagaacg
    76081 agagaaattg ggatcaaaaa agcgcttggc gctagacgta agcttatttt aaaacaattt
    76141 ttaattgaag cggtcattct aaccttgcta ggtggtgtga taggggttat ttcagggatg
    76201 gtatcaggat tgatcatcac aagatcactc gagtatccct atatcctatc cttgttctca
    76261 gttgtcttga gtttagcgtt ttgttgtatt atagggattg tctttggact attaccagcc
    76321 attaaagcgt ctaagctaga cccaattgaa gcattgcgat ttgaataatc aaccaaacaa
    76381 gcataaaagt caaaaaggat gaccctttga tgtcatcctt agctgcttga tacactaaac
    76441 gccaatctag aaagggaagt tatgtttaaa atattagtag ttgaagatga tgataccatc
    76501 agtcaagtga tttgtgagtt tttaaaagcg aataactatg atccagattg tgtatttgat
    76561 ggccaagctg ccttagacaa gtggcagaca acgtcttatg atctgattat tttagacatc
    76621 atgctccctt ccttaagtgg cttagaagtg ctaaaaacaa ttcgaaagac atcagatgtc
    76681 cctattatta tgctaacggc attagatgac gaatacacac agcttgtaag cttcaaccat
    76741 ttgattagtg attatgtgac caaaccattt tcaccactca ttttaatcaa acgtatcgaa
    76801 aatgtattac gcgtctctac tccagatgaa aagcgccaaa tcggagactt acttgttgat
    76861 gagactgaac atagcgtcta ttggcaagga acattggtca aactaaccaa aaaagaatat
    76921 gacattattg attatttagc aaaacgtcac caaaaaattg tcaccagaga ccaattgatg
    76981 gatgacatat ggggctattc tgaactggat acgcgtgtcc ttgacaacca cattaagaac
    77041 cttcgtaaaa aaatgacggg tattcccctc aagacaatta caggcatggg ctatttattg
    77101 ggagagcgtg agtgagacta atcaaaaaaa cctttttggt catcaatggc ttaattattg
    77161 tagtggttac ctctattttg ttagtgcttt attttgcaat gccaatttat tacaccaaag
    77221 tcaaagacaa agaagttaag cgtgaatttg accaaacaag caagcaaatc aaaggcaaaa
    77281 cggtaaccga gattagagat atcctaacaa aaaaaatcaa caaagataat atttggtaca
    77341 gcttagtaga cagtgataac caactgctct atccttcctt gcagctgtta gacggcgtca
    77401 gtgaaagcaa agacagtcaa aatgttaata tcgtcaccac ctttgacaac tcttattcta
    77461 atgttaaggt gatgagtcaa aaagtaaccc tacgcgatgg aaaaaaaatg acattgcttg
    77521 ggcaatcttc acttcaaccc gtaacagatg ctagcaaagt tctccttgac ctctacccgt
    77581 cactattgat gttttcagtg accgtaggga gtattgtcgc ctacttatat agtaggactt
    77641 ctagccggcg tatccttagc atgtcacaaa ccgccaaaaa aatggtcaac ctagaaccaa
    77701 acttaacgtg taccattcac ggcaaagatg aaatagcaat gttggctagt gatattaacc
    77761 gtttgtatgc gagtctttca acgagtatca aatccttgca aaaagagtat gaaaaagctt
    77821 ctgattcaga aagagagaaa tctgagtttt tacgcatgac atcgcacgag ctaaagacac
    77881 caatcaccag tgttatcgga atgattgatg gaatgctcta taatgttggt gactttgcag
    77941 atcgtgacaa atatttgcga aaatgccgag acgtccttga agggcaagcc caactggttc
    78001 aatccatttt atctttatct aagattgaaa ccctagcttc tcaaaatcaa gaactgtttt
    78061 cactaaaaag cagcctagaa gaagaaatgg aagtctttct cgtcttatca gaactaaaac
    78121 atctcaaagt gactatcaat ctcgaagaac aattcgtcaa agccaataaa gtatacctac
    78181 taaaggcgat taaaaatatt attgacaatg cctttcacta taccaaatca ggcggccaag
    78241 tgatgattca actaaaagac aaccaactag tgattaaaaa tgaagcagag acattattga
    78301 cacaacagca gatgaaacag ttattccaac cgttttatcg accagattat agtcgtaaca
    78361 ggaaagacgg tggtacagga ttggggctgt ttatcaccca ccagattctt gatcagcatc
    78421 atctggctta tcgctttgtt gttcttgatc aaagatggat ggtatttacg attgattttc
    78481 catcccatca tgacgattga tccacactag taggacgaca aaagtcatca atcactaacc
    78541 aagccttgac ctttacccat cattgtttag gcaagtataa aaaagaggag agaaatcata
    78601 tatgaagaaa aggaaattgt tagcagtaac actattaagt accatactct taaacagtgc
    78661 agtgccatta gttgttgctg acacctcctt gcgtaataac acatcatcca ctgatcagcc
    78721 tactacagca gatactgata cggatgacga gagtgaaaca gcaaaaaaag acaaaaaaag
    78781 caaggaaaca gcgtcgcagc acgacaccca aaaagaccat aagtcatcac acactcacct
    78841 aaccccccct tcaaatgata ctaagcagac cgatcaggca tcatctgaag ctactgacaa
    78901 accaaataaa gacaaaaacg acaccaagca accagacagc agtgatcaat ccaccccatc
    78961 tcccaaagac cagtcgtctc aaaaagagtc acaaaacaaa gatggccgac ctaccccatc
    79021 acctgatcag caaaaagatc agacacctga taaaacacca gaaaaatcag ctgataaaac
    79081 ccctgaaaaa ggaccagaaa aagcaactga taaaacccca gaaccaaatc gtgacgctcc
    79141 aaaacccatc caacctcctt tagcagcagc tcctgtcttt gcaccgtgga gagaaagtga
    79201 caaagacctg agcaagctaa aaccaagcag tcgctcatca gcggcttacg tgagacactg
    79261 gacaggtgac tctgcctaca ctcacaacct gttgtcacgc cgttatggga ttactgctga
    79321 acagctagat ggttttttga acagtctagg tattcactat gataaagaac gcttaaacgg
    79381 gaagcgttta ttagaatggg aaaaactaac aggactagac gttcgagcta tcgtagctat
    79441 tgcaatggca gaaagctcac taggtactca gggagttgct aaagaaaaag gagccaatat
    79501 gtttggttat ggcgcctttg acttcaaccc aaacaatgcc aaaaaataca gcgatgaggt
    79561 tgctattcgt catatggtag aagacaccat cattgccaac aaaaaccaaa cctttgaaag
    79621 acaagacctc aaagcaaaaa aatggtcact aggccaattg gacaccttga ttgatggtgg
    79681 ggtttacttt acagatacaa gtggcagtgg gcaaagacga gcagatatca tgatcaaact
    79741 cgaccaatgg atagatgatc atggaagcac acctgagatt ccagaacatc tcaagataac
    79801 ttccgggaca caatttagcg aagtgcctgt aggttataaa agaagtcagc cacaaaacgt
    79861 tttgacctac aagtcagaga cctacagctt tggccaatgc acttggtacg cctataatcg
    79921 tgtcaaagag ttaggttatc aagtcgacag gtacatgggt aacggtggcg actggcagcg
    79981 caaaccaggc tttgtgacca cccataaacc taaagtgggc tatgtcgtct catttgcacc
    80041 aggccaagca ggagcagatg caacctatgg tcacgttgct gttgtagagc aaatcaaaga
    80101 agatggttct atcttaattt cagagtcaaa tgttatggga ctaggcacca tttcctatcg
    80161 gacgttcaca gctgagcagg ctcgtttgtt gacctatgtc gtaggggaca aactcccaag
    80221 accataaccc cacaaccaag aagctacctg cctgttgacc aatctcaaaa agagacagtg
    80281 tcaacttaga atgaggttca tcagatgata aagaaagtga ccaccccgag tcaaaaaata
    80341 aaaaaacgtg ttcgtaatgg ctatctccta aaactaggta cagcctgttt actcttaagt
    80401 atactcagtt atggcattgg tcttttgggc cagcctagca tggaaaatac gtttatggga
    80461 atagcgagtg ttgccatgtt aggaagtgtc tgtttcttta ttatctttgc tctaaaccgt
    80521 atctttgacg cactagagga caatttgcga gattagagta ataggtcaaa taatcttcca
    80581 ataatttaga gcacaagcta agttattgga agtttttttg tcttttgaga gccatgatgc
    80641 catcaaaaag tcaaacactc cttatgatgt gagagtcaca tgactaaaat taaaagcaaa
    80701 atagacccca aagccctgtt ttctcaaaac agccaacgaa aaaagacaag ttaaccagtt
    80761 cataaaaaac atcgtagaaa cgttttcaag agctaatgtt ggagtaaatt gactgaagta
    80821 tgatagaatt tttaatgaat gtgacaataa tgtcacagat aaaaaccgaa aacagcaaaa
    80881 ataattaaat tgaatataaa aaagttaatt aagtgagtga taatcaagaa atgaagttca
    80941 aaaaatataa ataaaataaa acacaagaac tgctaaaagc agtgaatgac ataaatgtcg
    81001 ctaaaaaaat agatagaaga gtctaaaatt tcagttttag gtcacgtttt atgaatggaa
    81061 atcagaatga tgaccttaag ttgtgatttg cgcgtcattg accgctttct tcgttttaat
    81121 gaaagaattt gaaagatatt ttatggtata cttttcctta atatggttca tacggacttg
    81181 ataaatctat ttacaaaaat aataaaaaat taagataagg acatgaagtt aatgcatgta
    81241 agtaaattgt ttactagcca acaatggaga gaattgaaac tgatctcata tttaacagaa
    81301 aactccaatg ctatcggagt taaagataag gagcttagca aggctctcaa tatttccatg
    81361 ttaacattac agtcatgcct cactaatatg cagtttatga aagaagtagg tggaattacc
    81421 tacaaagacg gctatattaa catttggtat caccagtgtt gtgggctaca agaggtctat
    81481 caaaaagctc ttcgagaatc gccttctttg aaattactgg agcttttgtt tttcagagac
    81541 tttagttcct tagaagagtt agctgaagag ctgtttgtca gcttatctac cctcaaacgc
    81601 ctcatcaaaa aaaccaacac ttacttgtct catacctttg ctattagtat cgtgacaagt
    81661 ccagtgcagg tgtcaggaga tgagaggcaa attcgcctgt tttacctcaa gtatttttca
    81721 gaagcctata aaatctcaga atggccattt ggggacattt taaacctgaa aaactgtgaa
    81781 cgcctattga gcttattgat caaagaagta gatgtcaaag ttcattttac gctatttcag
    81841 cacttaaaaa tcttaagtgg cgtcaacttg atccgttact acaaggggta ttcttgcagt
    81901 tataacaata aaaaaaccag tcaccgtttt tctcaactta tccaacattc ctcagaaatc
    81961 caggatcttt ctcgcttgtt ctacctcaaa tttgggttac atctcgacga gtataccata
    82021 gctgaaatgt tttctaacca tcttaacgac aagctagaaa ttggctgtgc ttttgagatt
    82081 atcaatcaag acccaatatc aggaggcaga caagtaacca actggatcca tctattagat
    82141 gagatggaaa tcaagctcaa tctcagcatc accaataaat acgaagttgc cgtaaccctc
    82201 cataacgctt cggtgttaaa cgaagaagac attacagcca attatttgct gtttgattat
    82261 aaaaagagtt acctgaactt ttaccaaaaa gaacaccctc gtatctatga ggcctttgtg
    82321 acaagtgtcg agaagctgat gcaggcagat aatgctcaag ttagcaaaga attgatcaat
    82381 cagctcactt attgtttctt tattacctgg gaaaatagtt tcttaaaagt aaatcaaaaa
    82441 gaaaaagtac gactcctagt gatagagaga agttacaata gtgtcggtaa ttttttgaaa
    82501 aagtacatcg gtgagttttt cagcattact aattttgatg agttagattg tctgacaatt
    82561 gatctagtag agattgaaaa acagtacgat gttatcgtga cagatgttat ggtgggtaaa
    82621 agcgaagagt tagagatttt ctttttctac aagatgatcc cagaagctat tattgataga
    82681 ttaaacgaat ttttgaacgt tagtttcaca gataacaatg ttatggtcaa aaacctcgaa
    82741 gccccttctc cttcaaaatc tcatagcgac aaagaggtac aaaagccaga aaagccagac
    82801 aattctgtta aacaagcaac atcagcaaca tcatcatagg atttcagacg tcatggtaag
    82861 aaaaaaagaa tcatttacag cccatagagg taaggtcaaa agctgaaaac agctcaaaaa
    82921 aactgacctt taccttttgg ctttttttat ttagaataat tttattggag agatgcttaa
    82981 tctaatttaa gcacaattct tagaaagtta agaaataagg agtaaacaat gtctaaaaga
    83041 aatccaaaca aacactactc actgagaaag ttaaaaacag gtactgcatc agtagcagta
    83101 gctttgacag ttttggggac tggattagca aacacaactg atgtaaaggc tgagactgag
    83161 catgttgatg ttgtgctttc agcaaaagaa gctaacaaag tattcgaaga gcgcaaagcc
    83221 ttggaaaaac aagcgcgtga tttgggtgac actattaatc acatgtcaca aaccattagt
    83281 gagcaaagcc gcaagattgc agcattaaag tctgaagcag aacttaaaaa ccaacaagct
    83341 cttgaagctt taaataataa aaatgagcag attgctaaat taaccaacga aaacgcacag
    83401 ttaaaagaag ccattgaagg ttatgtgcaa actatccaaa acgctagccg tgaaatcgca
    83461 gcaaaacaac aagaacttgc agctgcaaaa agccagttag aggcaaaaaa tgctgagatt
    83521 gaggcattga aacaacaaga tgcctctaag actgaggaaa ttgctaaatt gcaatcagaa
    83581 gcagcaactt tagaaaacct cctaggttca gctaagcgtg aattgactga tctgcaagct
    83641 aagctagata cagctaacac tgaaaaagaa aagctccaat cacaagcagc agccctagaa
    83701 aaacaactag aagcaactaa aaaagagtta gctgatttac aggctaaatt agcagcaacc
    83761 aaccaagaaa aagaaaagtt agaagctgaa gcaaaagctc ttaaagagca attagcaaaa
    83821 caagctgaag agcttgctaa gctaaaagca gataaagctt caggagctca aaaaccagat
    83881 actaaacctg gcaataaaga ggttccaaca agaccgtcac aaacaagaac aaacactaat
    83941 aaagctccta tggcgcaaac aaagagacaa ttaccgtcaa caggcgaaga aacaaccaac
    84001 ccattcttca ctgcagcagc tgcaacagtg atcgcatctg caggcgtact tgccctaaaa
    84061 cgcaaagaag aaaactaagc ccaatccaca ctatcttttc tagcccaaga aaaaaacaaa
    84121 aaaagaggaa gaccattcct ctttttttga acggttaaac agcaaaaagg tcaaaaaggt
    84181 actaaagtct caaaaacctg gtctttacct tttaccgctt attctttaga atagaattat
    84241 tagagagaag tcttagaaaa atgaggctaa ttccctaaag atgaaaaaat aaggagcaaa
    84301 taatggctag aaaagatacg aataaacagt attcgcttag aaaattaaaa acaggtacag
    84361 catccgtagc ggtcgctgtg gctgttttag gagcaggctt tgcaaaccaa acagaagtta
    84421 aggctgcgga gtctccaaaa agtactgaga cttctgctaa tggagctgat aaattagctg
    84481 atgcatacaa cacattgctt actgaacatg agaaactcag agatgagtat tatacattaa
    84541 ttgatgctaa agaagaagaa cctaggtata aagcattgag aggcgaaaat caagatcttc
    84601 gggaaaaaga aggaaaatac caggataaaa taaaaaaatt agaagaaaaa gagaaaaatt
    84661 tagaaaaaaa atcagaagat gtagaacgtc actatcttaa aaaactagat caagaacata
    84721 aagaacaaga agaacgtcaa aaaaatctag aagaactcga acgtcaaagt caacgagaaa
    84781 tagacaagcg ttatcaagaa caactccaaa aacaacaaca attagaaaca gaaaagcaaa
    84841 tctcagaagc tagtcgtaag agcttaagcc gtgaccttga agcgtctcgt gcagctaaaa
    84901 aagaccttga agctgagcac caaaaactca aagaggaaaa acaaatctca gacgcaagcc
    84961 gtcaaggcct aagccgtgac cttgaagcgt ctcgcgaagc taagaaaaaa gtagaagcag
    85021 acttagcaga agcaaatagc aaacttcaag cccttgaaaa actaaacaaa gagcttgaag
    85081 aaggtaagaa attatcagaa aaagaaaaag ctgagttaca agcaagacta gaagctgaag
    85141 caaaagctct taaagagcaa ttggctaaac aagctgaaga acttgctaaa ctaaaaggca
    85201 accaaacacc aaacgctaaa gtagccccac aagctaaccg ttctagatca gcaatgacgc
    85261 aacaaaagag aacgttaccg tcaacaggcg aagcagctaa cccattcttt acagcagcag
    85321 ctgcaacagt aatggtatct gcaggtatgc ttgctctaaa acgcaaagaa gaaaactaag
    85381 ctattagact gatgctaaag ttaagagaga atcaaatgat tctctctttt tgagtggcta
    85441 agtaactaac aatctcaatt agaccaaaaa atgggaatgg ttcaaaaagc tggcctttac
    85501 tccttttgat taaccatata taagtaaaaa cattaggaaa ataatagtaa tattaagttt
    85561 gtttcctcaa taaaatcaag gagtagataa tggctagaca acaaaccaag aaaaattatt
    85621 cactacggaa actaaaaacc ggtacggctt gagtagccgt tgctttgacc gttttgggtg
    85681 caggttttgc aaaccaaacg gaagtaagag ctgaaggggt aaacccgact acgaacttgc
    85741 cagagaaggc taaatatgcc gcagtgaaag atgagaatac tggtttacgt ggtgatcaga
    85801 aaaaattagt aaaaaaactt gaagaagaac aagagaagag caaaaatcta gaaaagcaaa
    85861 aacaggagtt agaaaaccaa gcccttaact ttcaagacgt aattgaaact caggaaaaag
    85921 aagatctcaa aacaacttta gctaagacta ctaaagaaaa tgagatttca gaagctagcc
    85981 gtaaagggtt aagccgagac ttagaagctt ctcgtgcagc taaaaaagag ctagaagcta
    86041 agcaccaaaa attagcagaa gaacatcaag tttcagagac tagccgtaag ggtctaagcc
    86101 gtgaccttga agcatcacgt gaagctaata agaaggttac atctgagtta acacaagcaa
    86161 aagctcaact ctcagcgctt gaagaaagta agaaattatc agaaaaagaa aaagctgagt
    86221 tacaagcaaa actagatgca caaggaaaag ccctcaaaga acaattagca aaacaaactg
    86281 aagagcttgc aaaactaaga gctgaaaaag cggcaggttc aaaaacacct gctaccaaac
    86341 cagctaataa agaaagatca ggtagagctg ctcaaacagc tacaagacct agccaaaata
    86401 aaggaatgag atcacaatta ccgtcaacag gcgaagcagc taacccattc tttacagcag
    86461 cagctgcaac agtgatggta tctgcaggta tgcttgctct aaaacgcaaa gaagaaaact
    86521 aagcctttag aacttggttt ttgtaacggt gcaatagaca aaagtaagca aggccaaaaa
    86581 ctgagaaagt cctaaaaagc tggcctttac ccctcaaaat taatgtttta taataaagat
    86641 gttagtaata taattgataa atgagataca tttaatcatt atggcaaaag caagaaaaat
    86701 agctgtatca tatgcaaata acccctgttt gctctttaaa aaagacgtta tccttatttc
    86761 tccacgcaca gatggacagc taggagagaa tcgtttgatt ctctcttttc ttaatggtca
    86821 taaagacaaa gtctctcatc atgaaaggac gacacattgc gtaaaaaaca aaaattacca
    86881 tttgataaac ttgccattgc gctcatgtct acgagcatct tgctcaatgc acaatcagac
    86941 attaaagcaa atactgtgac agaagacact cctgctaccg aacaagccgt agaaccccca
    87001 caaccaacag cggtttctga ggaagcacca tcatcatcaa aggaaactaa ccctccacaa
    87061 attcctgatg acgcagaaga aacagtagca gataaggcta atgatccagc ccctcaagct
    87121 cctgctaaaa ctgctgatac accagcaacc tcaaaagcga ctattaggga tttgaacgac
    87181 ccttctcagg tcaaaaccct gcaggaaaaa gcaggcaagg gagctgggac tgttgttgca
    87241 gtgattgatg ctggttttga taaaaatcat gaagcgtggc gtttaacaga caaaaccaaa
    87301 gcacgttacc aatcaaaaga agatcttgaa aaagctaaaa aagagcacgg tattacctac
    87361 ggcgagtggg tcaatgataa ggttgcttat taccacgact atagcaaaga tggtaaaacc
    87421 gctgtcgatc aagagcacgg cacacacgtg tcagggatct tgtcagggaa tgctccatct
    87481 gaaacgaaag aaccttaccg cctagaaggt gcgatgcctg aggctcaatt gcttttgatg
    87541 cgtgtcgaaa ttgtaaatgg actagcagac tatgctcgta actacgctca agctatcaga
    87601 gatgctgtca acttgggagc taaggtgatt aatatgagct ttggtaatgc tgcactagct
    87661 tacgccaacc ttccagacga aaccaaaaaa gcttttgatt atgccaaatc aaaaggtgtt
    87721 agcattgtga cctcagctgg taatgatagt agttttgggg gcaaaacccg tctacctcta
    87781 gcagatcatc ctgattatgg ggtggttggg acacctgcag cggcagattc aacattgaca
    87841 gttgcttctt acagcccaga taaacagctc actgaaactg ctacggtcaa aacagacgat
    87901 catcaagata aagaaatgcc tgttctttca acaaaccgtt ttgagccaaa caaggcttac
    87961 gactatgctt atgctaatcg tggtacgaaa gaggatgatt ttaaggatgt cgaaggtaag
    88021 attgccctta ttgaacgtgg cgatattgat ttcaaagata agattgcaaa cgctaaaaaa
    88081 gctggtgctg taggggtctt gatctatgac aatcaggaca agggcttccc gattgaattg
    88141 ccaaatgttg atcagatgcc tgcggccttt atcagtcgaa aagacggtct cttattaaaa
    88201 gacaatcccc aaaaaaccat caccttcaat gcgacaccta aggtattgcc aacagcaagt
    88261 ggcaccaaac taagccgctt ctcaagctgg ggtctgacag ctgacggcaa tattaagcca
    88321 gatattgcag cacccggcca agatattttg tcgtcagtgg ctaacaacaa gtatgccaaa
    88381 ctttctggaa ctagtatgtc tgcgccattg gtagcgggta tcatgggact attgcaaaag
    88441 caatatgaga cacagtatcc tgatatgaca ccatcagagc gtcttgattt agctaaaaaa
    88501 gtattgatga gctcagcaac tgccttatat gatgaagatg aaaaagctta tttttctcct
    88561 cgccaacaag gagcaggagc agtcgatgct aaaaaagctt cagcagcaac gatgtatgtg
    88621 acagataagg ataatacctc aagcaaggtt cacctgaaca atgtttctga taaatttgaa
    88681 gtaacagtaa cagttcacaa caaatctgat aaacctcaag agttgtatta ccaagcaact
    88741 gttcaaacag ataaagtaga tggaaaacac tttgccttgg ctcctaaagc attgtatgag
    88801 acatcatggc aaaaaatcac aattccagcc aatagcagca aacaagtcac cgttccaatc
    88861 gatgctagtc gatttagcaa ggacttgctt gcccaaatga aaaatggcta tttcttagaa
    88921 ggttttgttc gtttcaaaca agatcctaaa aaagaagagc ttatgagcat tccatatatt
    88981 ggtttccgag gtgattttgg caatctgtca gccttagaaa aaccaatcta tgatagcaaa
    89041 gacggtagca gctactatca tgaagcaaat agtgatgcca aagaccaatt agatggtgat
    89101 ggattacagt tttacgctct gaaaaacaac tttacagcac ttaccacaga gtctaaccca
    89161 tggacgatta ttaaagctgt caaagaaggg gttgaaaaca tagaggatat cgaatcttca
    89221 gagatcacag aaaccatttt tgcaggtact tttgcaaaac aagacgatga tagccactac
    89281 tatatccacc gtcacgctaa tggcaagcca tatgctgcga tctctccaaa tggggacggt
    89341 aacagagatt atgtccaatt ccaaggtact ttcttgcgta atgctaaaaa ccttgtggct
    89401 gaagtcttgg acaaagaagg aaatgttgtt tggacaagtg aggtaaccga gcaagttgtt
    89461 aaaaactaca acaatgactt ggcaagcaca cttggttcaa cccgttttga aaaaacgcgt
    89521 tgggacggta aagataaaga cggcaaagtt gttgctaacg gaacctacac ctatcgtgtt
    89581 cgctacactc cgattagctc aggtgcaaaa gaacaacaca ctgattttga tgtgattgta
    89641 gacaatacga cacctgaagt cgcaacatcg gcaacattct caacagaaga tcgtcgtttg
    89701 acacttgcat ctaaaccaaa aaccagccaa ccgatttacc gtgagcgtat tgcttacact
    89761 tatatggatg aggatctgcc aacaacagag tatatttctc caaatgaaga tggtaccttt
    89821 actcttcctg aagaggctga aacaatggaa ggcggtactg ttccattgaa aatgtcagac
    89881 tttacttatg ttgttgaaga tatggctggt aacatcactt atacaccagt gactaagcta
    89941 ttggagggtc actctaataa gccagaacaa gatggttcag atcaagcacc agacaaaaaa
    90001 ccagaaacta aaccagaaca agacggttca ggtcaagcac cagacaaaaa accagaagct
    90061 aaaccagaac aagacggttc aggtcaagca ccagacaaaa aaccagaagc taaaccagaa
    90121 caagacggtt caggtcaaac accaaataaa aaaccagaaa ctaaaccaga aaaagatagt
    90181 tcaggtcaaa caccaggtaa aactcctcaa aaaggtcaac cttctcgtgc tctagagaaa
    90241 cgatcttcta agcgtgcttt agctacaaaa gcatcaacaa gagatcagtt accaacgact
    90301 aatgacaagg atacaaatcg tttacatctc cttaagttag ttatgaccac tttcttcttg
    90361 ggattagtag ctcatatctt taaaacaaaa cgcactgaag attagtacaa ctgatcagat
    90421 ggctactatt gatgatgagt tagatcagtc ttcttttcaa aagaaggcat agcagtataa
    90481 aaatagatat gtgaggacaa tatgcgtaga gcagaaaata acaaacacag ccgctattcc
    90541 attcgcaaac tgagcgttgg ggtaacgagt atagcaattg cgagtctctt tttgggaaaa
    90601 gttgcctatg ccgtagatag catgcagcca atctcttcga ctcaaggaaa cagagccaat
    90661 acatccaaaa aatggtatga tattaatgag tatggtctta ttcctgtaga tataagcttt
    90721 gaagctgcca aaaaagattt taaagaagaa gtagaaaaat cacttttgtc tgaagaacaa
    90781 cagaaaacgt ataatcaaaa aattgaagct gcaagaaaca aagacgacct attgttaacg
    90841 tatcatagtg agtactctaa agatattaag aaacactcaa cgtctactga gccagttgag
    90901 gcatctgtgc aggagacaca ggcatcagtt tcagattcga tggtgacaga tgattcaaca
    90961 tcagttacgg ctgattctcc tgagaaaaca ccatctacag aaacaccggc tccatcaatt
    91021 ccagaggtcc cagaagcccc agcttcatca cctgagagtg aggagtcttc agtagcatct
    91081 tctgaggaaa cctcatctcc agaaactcca gtggccccag aagccccagc tttatctgag
    91141 gctccagctc aaccagctga gagtgaggag tcttcagtag cagctccttc tgaggaaacc
    91201 ccatctccag aaactccagc ggccccagaa actcctgaag agccagcagc tccatctcca
    91261 tcacctgaga gtgaggaacc ttcagtagca gcttcttctg aggaaacccc atctccagaa
    91321 actccagcgg ctccagaaac tcctgaagaa ccagcagctc cagctcaacc agctgagagt
    91381 gaagaatctt cagtagcagc tacgacaagc ccgtctccat caactccagc cgaatcagag
    91441 actcagacgc caccagctgt tactaaagac tctgataagc catcttcagc agctgaaaaa
    91501 ccagcagcct cttcacttgt ttcagaacaa accgttcaac aaccaacttc aaagagatct
    91561 tctgataaaa aagaagagca agaacagtct tactctccaa atcgctcatt gtcaagacag
    91621 gttagggccc atgagtcagg taagtacttg ccttcaacag gtgaaaaagc acagccactc
    91681 tttatagcta ctatgacttt gatgtctcta tttggcagtc ttttagtcac aaaacgccaa
    91741 aaagaaacta aaaaatagta ttacttaaac tgaatagtat tacttaaact gataagagct
    91801 tatttaatag agagaaccaa tttggttctc tctatttttt tagatgtgct gcctcatcaa
    91861 aaagaagtta ggacaaatag ctgtcaacaa ttgtcttttg cttatctctt cagttgctgt
    91921 gattttatca tatattgata aaagatggca cgccaaacgc atgaagaaac tttttttttc
    91981 agaaaactag attcataatg acaaaatcaa tcaaagtagt acactataga tgaggtgact
    92041 acgatgattg attttattat ttctattgat gattg
//
LOCUS       DATXIG010000002        86231 bp    DNA     linear   BCT 28-DEC-2023
DEFINITION  TPA_asm: Streptococcus pyogenes strain MGAS11113
            SAMN08555799-rid19682583.denovo.002, whole genome shotgun sequence.
ACCESSION   DATXIG010000002 DATXIG010000000
VERSION     DATXIG010000002.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN08555799
            Sequence Read Archive: SRR6740495
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pyogenes
  ORGANISM  Streptococcus pyogenes
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 86231)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 86231)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-OCT-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-OCT-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001918058.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001918058.2
            Genome Coverage       :: 368x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/12/2023 11:23:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 1,806
            CDSs (total)                      :: 1,769
            Genes (coding)                    :: 1,722
            CDSs (with protein)               :: 1,722
            Genes (RNA)                       :: 37
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1, 1 (5S, 23S)
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 47
            CDSs (without protein)            :: 47
            Pseudo Genes (ambiguous residues) :: 0 of 47
            Pseudo Genes (frameshifted)       :: 30 of 47
            Pseudo Genes (incomplete)         :: 20 of 47
            Pseudo Genes (internal stop)      :: 9 of 47
            Pseudo Genes (multiple problems)  :: 11 of 47
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..86231
                     /organism="Streptococcus pyogenes"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN08555799-rid19682583.denovo.002"
                     /strain="MGAS11113"
                     /serotype="Emm28"
                     /isolation_source="normally sterile site"
                     /host="Homo sapiens"
                     /db_xref="taxon:1314"
                     /country="Finland"
                     /collection_date="2002"
                     /collected_by="National Public Health Laboratory"
     gene            24..461
                     /locus_tag="VC122_000074"
     CDS             24..461
                     /locus_tag="VC122_000074"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009880939.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CopY/TcrY family copper transport repressor"
                     /protein_id="HEP5640035.1"
                     /translation="MNQNISAAEWEVMRVVWASGDIKSSDIITILRKKYQWSDSTIKT
                     LIGRLVEKKALGTYRQGRAYIYQALLDETLLQKEALATVLDGICQRQHTHLLLERLYD
                     LPMTLEEIEAFQELLEVKKENAVLEVSCNCLPGQCHCQKKETY"
     gene            461..2692
                     /locus_tag="VC122_000075"
     CDS             461..2692
                     /locus_tag="VC122_000075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011528893.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="copper-translocating P-type ATPase"
                     /protein_id="HEP5640036.1"
                     /translation="MAKEIFLVEGMSCASCALTIEKMVNQLPEVEQAVVNLATEKLTV
                     TYQDDDLETAKVIQAVKAAGYGAQVFDEHAKHMQTGRRDEDLKVIWSQVLWSALFTIP
                     ILYLAMGHMVGLWLPPFLQPDRYPFVYSFSQLLLTIPVLVLNRHYYHNGFKALFKGYP
                     NMDSLVALATSFAFAYSLYGVVEISLGQANFVHSLYFESVVVILTLIGLGKYFESRSK
                     GRTSQAIQKLLSLKATVVRVWRNSQWQLLPLEEVSYDDLVLVQPGEKVSIDGIIVEGH
                     SSLDESFLTGESLPVEKGEKDNVFAGSLNGPGALTIKPDKLGNDTLLAQIIQLVENAQ
                     ENKAPIAAIADKVSGVFVPVVLVLALLTGVFWLVFMQESLRFSLTTAIAVLVIACPCA
                     LGLATPTAIMVGTGRAAENGILFKGGDILEQAHQVDTVVFDKTGTITEGKPSLQKLLT
                     FSGDDHLILQEAASLEAYSQHPLGEAIVRAAQEAGYDSLPVEEFESLTGLGVTGQLEG
                     RRLAIGNAALMGILGIDLSCVQSVCAKASEKGQTLVYYAKEGQLRALFSIADAVKEDS
                     QATVEALHQLGIHTIMLTGDHDATAKAIASQVGITDVISQVLPDQKAGVIADLRSQGR
                     KVAMVGDGINDAPALAVADIGIAMGSGTDIAIESADVILMKPDMLDLVKAMSLSRATM
                     RIVKENLFWAFIYNVLMIPVAMGLLHLFGGPLLNPMLAGFAMSFSSVSVVLNALRLKG
                     KTI"
     gene            2706..2909
                     /locus_tag="VC122_000076"
     CDS             2706..2909
                     /locus_tag="VC122_000076"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014635648.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heavy-metal-associated domain-containing
                     protein"
                     /protein_id="HEP5640037.1"
                     /translation="MEKHYQVTGMTCDGCARTVTEKLSAVPGVQSVQVNLEKGQAKVT
                     GRPLTFLLKRALKDTKFELHQSS"
     gene            complement(3113..3883)
                     /locus_tag="VC122_000077"
     CDS             complement(3113..3883)
                     /locus_tag="VC122_000077"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002988830.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DeoR/GlpR transcriptional regulator"
                     /protein_id="HEP5640038.1"
                     /translation="MLKRERLLKITEIVNEQGIVTVNDIIQTLNVSDMTVRRDLDELE
                     KAGKLIRIHGGAQSITMPNKKERSNIEKQTVQTKEKWELASYATQLVNDGETIFIGPG
                     TTLECFAEQLKNRQIRIVTNSLPVFNILQDSETIDLILIGGEYRSITGAFVGSLASQN
                     ISSLKFAKAFISCNGIYKNDIATYSETEGEIQKLAFNNSIEKYLLVDNQKFNAYDFFI
                     FYHLNNIDAVVTDSQITEDVIERYSQFTQLLVAHENKE"
     gene            4120..4593
                     /locus_tag="VC122_000078"
     CDS             4120..4593
                     /locus_tag="VC122_000078"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009881202.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HEP5640039.1"
                     /translation="MFPNILFTEARTQPELFDLVASYLEKVGYVTQEYHQALVEREAV
                     FPTGLKVDLKDGSDILYAAIPHTETKYCLVDQVVYVRNSQALTFKHMINPEEDCLVTD
                     FFFIINSQNEGQTTILSNLITFFITKGNLSYLASLKDDKQAISNYLIEKGVFQHD"
     gene            4586..4891
                     /locus_tag="VC122_000079"
     CDS             4586..4891
                     /locus_tag="VC122_000079"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002983511.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HEP5640040.1"
                     /translation="MIKILAACGAGVNSSHQIKDAIETQMSDRGYDVHCDAVMVKDIT
                     EELVSRYDIFTPIAKTDLGFEMPIPTVEAGPILYRIPIMSEPVFAELERVIKENHLQ"
     gene            4917..6368
                     /locus_tag="VC122_000080"
     CDS             4917..6368
                     /locus_tag="VC122_000080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002994298.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS galactitol transporter subunit IIC"
                     /protein_id="HEP5640041.1"
                     /translation="MMQPFLDIINKILGFPIQLGSGVVMLIVMTGLAMIFGVKFTKAL
                     EGGIKLAIALTGIGAIIGILTGAFSESLQAFVKNTGISLNIIDVGWAPLATITWGSPY
                     TLYFLLVMLVVNIVMIVMKKTDTLDVDIFDIWHLSITGLLIMWYADHNHLPVLVSLLI
                     ATVAVILVGVLKIINSDLMKPTFDDLLGTGSQSPMTSTHMNYMMNPIIMVLDKIFDKV
                     FPGLDKYDFDAAKLNKKIGFWGSKFFIGMALGFVIGIMGDPHFTVESIKNWFGLGFTA
                     GACLELFSLIGSWFIAAVEPLSQGITNFANARMHGRRFNIGLDWPFIAGRAEIWACAN
                     ILAPIMLIEAVLLSKVGNGILPLAGIIAMGMTPALLVVTRGRLIRMIIFGSLLLPLFL
                     LSGTMIAPFATELAKKVGAFPAGTSAGSLITHSTLEGPMEKIFGYVIGQATTGQIASI
                     ITLIIFVAIYLGLFAWYANQMKARNAEYAKTMK"
     gene            6625..7050
                     /gene="lacA"
                     /locus_tag="VC122_000081"
     CDS             6625..7050
                     /gene="lacA"
                     /locus_tag="VC122_000081"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003044905.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="galactose-6-phosphate isomerase subunit LacA"
                     /protein_id="HEP5640042.1"
                     /translation="MAIILGADAHGNALKELIKSFLQEEGYDIIDVTDINSDFIDNTL
                     AVAKAVNEAEGRLGIMVDAYGAGPFMVATKLKGMVAAEVSDERSAYMTRGHNNARMIT
                     IGAEIVGPELAKNIVKGFVTGPYDGGRHQIRVDMLNKMA"
     gene            7065..7579
                     /gene="lacB"
                     /locus_tag="VC122_000082"
                     /pseudo
     CDS             7065..7579
                     /gene="lacB"
                     /locus_tag="VC122_000082"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002994294.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="galactose-6-phosphate isomerase subunit LacB"
     gene            7589..8042
                     /locus_tag="VC122_000083"
                     /pseudo
     CDS             7589..8042
                     /locus_tag="VC122_000083"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003061423.1"
                     /note="catalyzes the formation of tagatose
                     1,6-bisphosphate from tagatose 6-phosphate and ATP;
                     frameshifted; incomplete; partial in the middle of a
                     contig; missing C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="tagatose-6-phosphate kinase"
     gene            8089..9066
                     /gene="lacD"
                     /locus_tag="VC122_000084"
     CDS             8089..9066
                     /gene="lacD"
                     /locus_tag="VC122_000084"
                     /EC_number="4.1.2.40"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011054918.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tagatose-bisphosphate aldolase"
                     /protein_id="HEP5640043.1"
                     /translation="MTITANKRHYLEKVSHQGIISALAFDQRGALKQMMAAHQEGEAT
                     VTQIETLKVLVSEELTPYASSILLDPEYGLLATKVRANQTGLLLAYEKTGYDATTTSR
                     LPDCLVEWSVKRLKAAGADAIKFLLYYDVDGDEQINLQKQAYIERIGSECTAEDIPFF
                     LELLSYDERISDNNSAAYAKLKPHKVNGAMSVFSDKRFGVDVLKVEVPVNMAYVEGFT
                     EGEVHYSQAEAIKAFQDQEAASHLPYIYLSAGVSAKLFQETLYFAAAAGAQFSGVLCG
                     RATWAGSVPVYITKGEDEARKWLCTEGFQNIDELNRVLEETASPWTEKI"
     gene            9263..9880
                     /locus_tag="VC122_000085"
     CDS             9263..9880
                     /locus_tag="VC122_000085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014407775.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="trimeric intracellular cation channel family
                     protein"
                     /protein_id="HEP5640044.1"
                     /translation="MTIDMWEILNIIGTIAFALSGAIVAMEEEFDILGIFILGFVTAF
                     GGGAIRNTLIGLPIEALWGQKPEFTCAFFAMVLIMLFPKLIARGWVRAVVLTDAIGLA
                     AFSVQGALHAVRLNQPLSAVIVAAVLTGAGGGVVRDILAGRKPSVLRSEIYAGWSILA
                     AIVLHFKLADSTIECYALVVLLTTLRMIGNRKKWNLPKIKWKEER"
     gene            9882..10706
                     /locus_tag="VC122_000086"
     CDS             9882..10706
                     /locus_tag="VC122_000086"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010922570.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Cof-type HAD-IIB family hydrolase"
                     /protein_id="HEP5640045.1"
                     /translation="MIQLIAIDLDGTLLNQDKQIPKENITAIQEAAQSGLKIVLCTGR
                     PQSGTRPYFDQLGLTQEEFLIINNGCSTYSSPDWQLRHSKMLKVSDIELLEELSQSFP
                     DIYLTLTEENDYLVLEEEVPDLVQEDGDLVFTIVKPVSLAELSDTPRLIFQAMYLGEK
                     AALDAFERAVRNQLSQSFHVVRSQDNILEILPQGVSKASALKELVEDLGLTADQVMAI
                     GDAPNDIEMLTYAGLGVAMENASAAIKPLADKVTLTNDMAGVAQAIRQFALAAQKD"
     gene            complement(10742..11299)
                     /locus_tag="VC122_000087"
     CDS             complement(10742..11299)
                     /locus_tag="VC122_000087"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010922569.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HEP5640046.1"
                     /translation="MTDKALSKRSLQNLTQFNKETRRIARESMEIALLNLLETKPLGD
                     ITISELVTKAGVSRNAFYRNYTSKEAIIEQLLVGVIRRIFRGLKQFDIKTQAYQAWLY
                     LFTEAKKEAQLLKMIFKHHLHHLLTRLVTKRLKAYQKLKDKKQSHYKRLFWSNAIVSV
                     LTNWISDDMIVPAEEMAAMGLPLLT"
     gene            11592..12434
                     /locus_tag="VC122_000088"
     CDS             11592..12434
                     /locus_tag="VC122_000088"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003054136.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DegV family protein"
                     /protein_id="HEP5640047.1"
                     /translation="MTWKIVTDSGCDLRSLTRQSKELRFERVPLTLQIGTEIFRDDDG
                     LDIDNMMTTMYQSSKATTSSCPSPEAFLQAYRGADNVIVMTITGTLSGSHNSARLAKN
                     ELLEENPNVNIHLIDSLSAGGEMDLLVLELERLINLGLSFEEVVKQITAYQQKTRLIF
                     VLAKVDNLVKNGRLSKLVGKVIGLLNIRMVGKASNKGTLELLQKARGQKKAVSALIEE
                     IQKEGYVGGKVYIAHAQNPKICEQISEKIKSLYPDAVIQTGRTSGLCSFYAEDGGLLM
                     GYEI"
     gene            complement(12563..13285)
                     /locus_tag="VC122_000089"
     CDS             complement(12563..13285)
                     /locus_tag="VC122_000089"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002994282.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEP5640048.1"
                     /translation="MATMKQYHKGDSMTKSQKEALYWMLSVLTITLIGGSCLIFGSHP
                     QTQDKVAKHSKSAASLLKKAVKAVNDADRLATAAAIQEAQKAVDKLAESSKKKTLQEQ
                     LNVAKAKQEQEDAATQAVKAAEETLNQNLKDIAQKAVNDLSNKGKKAALQSRLDAILP
                     AKPIIDEFPRQSGEITDNSYWTPFPGDVSDTYDNSQSPTLDPSSESSASDVTPQPSHP
                     DPIPPQTSSEPSDSGDKQSSKE"
     gene            13487..15118
                     /locus_tag="VC122_000090"
     CDS             13487..15118
                     /locus_tag="VC122_000090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002983536.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Na/Pi cotransporter family protein"
                     /protein_id="HEP5640049.1"
                     /translation="MSVNWQDIAFHFFGGLGLFLFSIKYMGDGLQQAAGDKLRYYIDK
                     YTSNPFFGILVGIAMSALIQSSSGVTVITVGLVSAGLLNLRQAIGIVMGANIGTTITS
                     FLIGFKLGDYALPMIFIGAACLFFTSNKKLNNFGRIIFGVGGIFFSLNLMGDAMDPLK
                     SVSAFQNYLATLGDKLFQGVFIGTALTMLIQSSAAIIGILQGLFSGGLLTLQGAIPIL
                     LGSNIGTCITAVLAAIGSNIAAKRVAAAHVLFNLIGTIIFMIILVPFTSLMLWLQSKL
                     SLTPEMTIAFSHGSFNITNTILLIPFISLLAMIVTRLIPGEDEVVKYEALYLDRLLIT
                     QAPSIALGNAHKELVHLASYAIQAFEASYSYIMTADGKFGEKVKRYERAVDTIDEELT
                     TYLVDISNEALSPSENEVLAGILDSSRDLERIGDHSESLGILIEGIISKQIGFSISAR
                     QELTEMYQLTHCLTLDAIRAIVDSDTDLAQTIVTRHKEIEEKERRLRKTHIKRLNCGE
                     CTAQAGINFIDIISHYTRITDHALNLAEKVLSHQL"
     gene            15235..16383
                     /gene="nagA"
                     /locus_tag="VC122_000091"
     CDS             15235..16383
                     /gene="nagA"
                     /locus_tag="VC122_000091"
                     /EC_number="3.5.1.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014635640.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylglucosamine-6-phosphate deacetylase"
                     /protein_id="HEP5640050.1"
                     /translation="MTCYLKADCFYYPTEVKPAGYLGLHDGVFGEWTEIVPADAQIID
                     YTGYQIAPGLVDTHIHGYAGADVMDNSAQGIHQMSEGLLATGVTSFLPTTLTSTFEQL
                     EKVSGTIASVADQVKGAKIQGIYFEGPYFTEEYKGAQNPSYMKTPRLEEFDAWQKAAK
                     GLIKKIALAPEREGVKEFVSAVTKQGVTVALGHSNGTYQEAKEAVQAGASVWVHAYNG
                     MRGLTHREPGMVGAVYNLPNTYAELICDGHHVSPIACDILMQQKGHDHVAMITDCMRA
                     GGSPDGDYLLGEFSVVVANGTARLKESGNLAGSILKLKDGLRNVVAWGIATPAEAIHM
                     ATYVPAVSVGIDDVCGQIKAGHAADFIVLDKDLTLVATYLNGQKAFDA"
     gene            16505..17344
                     /locus_tag="VC122_000092"
     CDS             16505..17344
                     /locus_tag="VC122_000092"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011285666.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldo/keto reductase"
                     /protein_id="HEP5640051.1"
                     /translation="MVTTVKMTSGYEIPVLGFGTYQAADGEEAYQSTLAAIKAGYRHI
                     DTAAIYKNEESVGRAIKDSGVLREDLFITTKLWNDAHSYEGAKDALAASLDRLGLDYV
                     DLYLIHWPNPKALRNTWKEANAQAWQYMEEAVEAGLIKSIGVSNFMVHHLEALQETAK
                     ITPAINQIRLAPGCYQKEVVDYCKANEILLEAWSPLGQGEIFDNETMQQLANKYDKTV
                     AQVALAWSLAEGFIPLPKSVHDERIKENMAIFDVSLTQEDKKTIRYLSGMSAIPNPDT
                     TSF"
     gene            17476..18009
                     /locus_tag="VC122_000093"
     CDS             17476..18009
                     /locus_tag="VC122_000093"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002983542.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="isoprenylcysteine carboxyl methyltransferase
                     family protein"
                     /protein_id="HEP5640052.1"
                     /translation="MTIFLVMLAMFTIRLVFLKKSITNEKAILAQGGQEFGAQNTKFL
                     TLLHIMIYVFAVIEALLKQIKFDGISFLGLLLMLLSVAVLYEVTRILGDIWTVKLMLA
                     KDHKYVDHWLFKTIKHPNYFLNIAPELVGIALLCHAKITAMLLFPCYIVVIYLRIREE
                     NKLLAEVIIPNGTRTKP"
     gene            18381..19298
                     /gene="glyQ"
                     /locus_tag="VC122_000094"
     CDS             18381..19298
                     /gene="glyQ"
                     /locus_tag="VC122_000094"
                     /EC_number="6.1.1.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018163989.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine--tRNA ligase subunit alpha"
                     /protein_id="HEP5640053.1"
                     /translation="MSKKLTFQEIILTLQQYWNDQGCMLMQAYDNEKGAGTMSPYTFL
                     RAIGPEPWNAAYVEPSRRPADGRYGENPNRLYQHHQFQVVMKPSPSNIQELYLASLEK
                     LGINPLEHDIRFVEDNWENPSTGSAGLGWEVWLDGMEITQFTYFQQVGGLATSPVTAE
                     VTYGLERLASYIQEVDSVYDIEWAPGVKYGEIFLQPEYEHSKYSFEISDQDMLLENFE
                     KFEKEASRALEEGLVHPAYDYVLKCSHTFNLLDARGAVSVTERAGYIARIRNLARVVA
                     KTFVAERKKLGFPLLDEATRAILLAEDDE"
     gene            19676..21715
                     /locus_tag="VC122_000095"
     CDS             19676..21715
                     /locus_tag="VC122_000095"
                     /EC_number="6.1.1.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019418828.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine--tRNA ligase subunit beta"
                     /protein_id="HEP5640054.1"
                     /translation="MSKNLLIELGLEELPAYVVTPSEKQLGERLATFLTENRLSFEDI
                     QTFSTPRRLAVRVSGLADQQTDLTEDFKGPAKKIALDADGNFSKAAQGFVRGKGLTTD
                     AIEFREVKGEEYVYVTKHEAGKPAKEVLLGVTEVLSAMTFPVSMHWANNSFEYIRPVH
                     TLTVLLNDEALELDFLDIHSGRVSRGHRFLGTETTITSADSYEADLRSQCVIVDAKER
                     QEMIVEQIKTLEVEQGVQVDIDEDLLNEVLNLVEFPTAFMGNFEAKYLDVPEEVLVTS
                     MKNHQRYFVVRDQAGHLMPNFVSVRNGNDQAIENVIKGNEKVLVARLEDGEFFWREDQ
                     KLQIADLVAKLTNVTFHEKIGSLAEHMDRTRVIAASLAKEANLSAEEVTAVDRAAQIY
                     KFDLLTGMVGEFDELQGIMGEKYALLAGEDAAVATAIREHYLPDAAGGALPETKVGAV
                     LALADKLDTLLSFFSVGLIPSGSNDPYALRRATQGIVRILDHFGWRIPMDKLVDSLYD
                     LSFDSLTYTNKADVMNFIRARVDKMMGKAAPKDIREAILASSTFVVPEMLAVAEALVK
                     ASHTENYKPAVESLSRAFNLAEKADASVQVDPSLFENEQENTLFAAIQGLTLAGSAAQ
                     QLEQVFALSPVINDFFDNTMVMAEDQALKNNRVAILSDLVSKAKTIAAFNQLNTK"
     gene            21869..22126
                     /locus_tag="VC122_000096"
     CDS             21869..22126
                     /locus_tag="VC122_000096"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002983550.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF896 family protein"
                     /protein_id="HEP5640055.1"
                     /translation="MDPKKIARINELAKKKKTVGLTGPEKVEQAKLREEYIEGYRRSV
                     RHHIEGIKLVDEEGNDVTPEKLRQVQREKGLHGRSLDDPKS"
     gene            22253..22645
                     /locus_tag="VC122_000097"
     CDS             22253..22645
                     /locus_tag="VC122_000097"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011054910.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PepSY domain-containing protein"
                     /protein_id="HEP5640056.1"
                     /translation="MCLSYNRRNQRKKVVLMTIKKFSLLAIASLSLLSLAACDMDDKD
                     DHMDNQPKTSQTSKKVKLSEDKAKSIALKDASVTEADAQMLSVTQDNEDGKAVYEIEF
                     QNKDQEYSYTIDANSGDIVEKSSEPIND"
     gene            23005..24531
                     /gene="glpK"
                     /locus_tag="VC122_000098"
     CDS             23005..24531
                     /gene="glpK"
                     /locus_tag="VC122_000098"
                     /EC_number="2.7.1.30"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011285068.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerol kinase GlpK"
                     /protein_id="HEP5640057.1"
                     /translation="MSQEKYIMAIDQGTTSSRAIIFNQKGEKVSSSQKEFPQIFPHAG
                     WVEHNANQIWNSVQSVIAGAFIESSIKPSQIEAIGITNQRETTVVWDKKTGVPIYNAI
                     VWQSRQTAPIAEQLKQDGHTKMIHEKTGLVIDAYFSATKIRWILDHVPGAQERAEKGE
                     LLFGTIDTWLVWKLTDGAVHVTDYSNAARTMLYNIKDLTWDDEILELLNIPKDMLPEV
                     KSNSEIYGKTAAFHFYGGEVPISGMAGDQQAALFGQLAFEPGMVKNTYGTGSFIIMNT
                     GDEMQLSSNNLLTTIGYGINGKVHYALEGSIFIAGSAIQWLRDGLKMIETSPESEQLA
                     LASTSDDEVYVVPAFTGLGAPYWDSNARGSVFGLTRGTSKEDFVKATLQSIAYQVRDV
                     IDTMQVDSGIDIQQLRVDGGAAMNNMLMQFQADILGIDIARAKNLETTALGAAFLAGL
                     AVGYWEDMDALKELNATGQLFKASMNESRKEKLYKGWKRAVKATQVFTQEEDADDDAK
                     "
     gene            24547..26385
                     /gene="glpO"
                     /locus_tag="VC122_000099"
     CDS             24547..26385
                     /gene="glpO"
                     /locus_tag="VC122_000099"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003059148.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type 1 glycerol-3-phosphate oxidase"
                     /protein_id="HEP5640058.1"
                     /translation="MEFSRETRRLALQKMQERDLDLLIIGGGITGAGVALQAAASGLD
                     TGLIEMQDFAQGTSSRSTKLVHGGLRYLKQFDVEVVSDTVSERAVVQQIAPHIPKPDP
                     MLLPVYDEPGSTFSMFRLKVAMDLYDLLAGVSNTPAANKVLTKEEVLKREPDLKQEGL
                     LGGGVYLDFRNNDARLVIENIKRANRDGALIASHVKAEDFLLDDNGKIIGVKARDLLS
                     DQEIIIKAKLVINTTGPWSDETRQFSHKGQPIHQMRPTKGVHLVVDRQKLPVSQPVYV
                     DTGLNDGRMVFVLPREEKTYFGTTDTDYTGDLEHPQVTQEDVDYLLGVVNNRFPNANV
                     TIDDIESSWAGLRPLLSGNSASDYNGGNSGKVSDDSFDHLVDTVKAYINHEDSREAVE
                     KAIKQVETSTSEKELDPSAVSRGSSFDRDENGLFTLAGGKITDYRKMAEGALTGIIQI
                     LKEEFGKSFKLINSKTYPVSGGEINPANVDSEIEAYAQLGTLSGLSMDDARYLANLYG
                     SNAPKVFALTRQLTAAEGLSLAETLSLHYAMDYEMALKPTDYFLRRTNHLLFMRDSLD
                     ALIDPVINEMAKHFEWSDQERVAQEDDLRRVIADNDLSALKGQQEG"
     gene            26387..27088
                     /locus_tag="VC122_000100"
     CDS             26387..27088
                     /locus_tag="VC122_000100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003044848.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aquaporin family protein"
                     /protein_id="HEP5640059.1"
                     /translation="MDIFGEFLGTALLVLLGNGVVAGVVLPKTKTHASGWIVIATGWG
                     IAVAVAVFISGKVAPAHLNPAVSLAFAMSGTIAWSTAIAYSLAQLLGAMVGSTLVFLQ
                     FRPHYLAAESQADILGTFATGPAIRDTSSNLLSEIFGTFVLMLGILAFGLYDMPAGLG
                     TLCVGTLVIGIGLSLGGTTGYAINPARDLGPRLVHAILPLNNKGDSDWSYAWIPVVGP
                     IIGAALAVLLFQVMS"
     gene            27197..28543
                     /locus_tag="VC122_000101"
     CDS             27197..28543
                     /locus_tag="VC122_000101"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010922562.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HEP5640060.1"
                     /translation="MTLTTKTIHVIGASFAGLAFVDKYKDLNPDSQIILIDKESCPNY
                     IPNGINQLFRGDIQDLSDAMWGRACLAAQIESNHRFIQAEVLAIEAPSNTLLLKDSQG
                     RVFEEGYETLVCAMGASPQSHYIETSQTNKVLVTKYYEESQASLKLIEASQEVLVIGA
                     GLIGLDLAYSLSLQGKRVKLIEAAERPDFYQTDAELIAPVMAEMSTHHVTFINNKRVT
                     AIHEIEGKVVAHTEQGDTFQGDLAILAINFRPNTHLLQGQVACALDKTILVNENLQTS
                     QANIYAIGDMVSLYFGILGMDYYTPLINQAMKTGQALALHLAGYPIPPLQTVKVLGSS
                     HFGYYRASVGVTEEEAELYMDTCSYLYQNGDSKNLFWLKLIARKTDGILIGAQLLSKT
                     NALVIANQLGQALALKVTDGDLAFQDFLFLQGHSDLAYHLHEACLKLFEKRLRHED"
     gene            28533..30032
                     /locus_tag="VC122_000102"
     CDS             28533..30032
                     /locus_tag="VC122_000102"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011285664.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HEP5640061.1"
                     /translation="MKIEDLMDKERKAQYRLLVTLYHAKETLRLKDLMRLSNLSKVTL
                     LKYIDNLNHLCREQGLACQLLLEKDSLSLKENGQFHWEDLVALLLKESVAYQILTYMY
                     CHEHFNITNLSVELMVSEATLNRQLAHLNQLLSEFDLALSQGRQLGSELQWRYFYLEL
                     FRHTLTRQGIDALVNQLDASHLATLIERLIGQSLSAEALEQLLIWLAISQARMSFQKG
                     YNDHFLRDSDFMTSNIFFKRLESMLLHYLRRYALEFDAFEAKSLFVFLHAYPLLPIAS
                     MKYSLGFGGPIADHISEALWLLKKAHVIIHQTKEEIIYGLGIFFSKAYFFKGAILSQP
                     TNSQYLYQLVGEDKRALLRVIINHLVLQMGQETDFSQQLSDDILALLVFSIERHHEPL
                     LVGLALGQNKVEAAIAELAIRRHLGHRRDFQLMPYDHQKVYDCLITYQTVYLPRQDLP
                     YYRLKQYSSPYELTALEAFLKDLFQQKNTREEELSLSPAAKSSFAHKTV"
     gene            30158..30802
                     /gene="fsa"
                     /locus_tag="VC122_000103"
     CDS             30158..30802
                     /gene="fsa"
                     /locus_tag="VC122_000103"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012657835.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fructose-6-phosphate aldolase"
                     /protein_id="HEP5640062.1"
                     /translation="MKFFLDTANVAAIKAINELGVVDGVTTNPTIISREGRDFETVIK
                     EICDIVDGPISAEVTGLTADAMVEEARSIAKWHDNVVVKIPMTTEGLKATNILSKEGI
                     KTNVTLIFTVSQGLMAMKAGATYISPFIGRLEDIGTDAYQLISDLREIIDLYDFQAEI
                     IAASIRTTAHVEAVAKLGAHIATIPDPLFAKMTQHPLTTNGLKTFMEDWASFKK"
     gene            31020..33005
                     /gene="tkt"
                     /locus_tag="VC122_000104"
     CDS             31020..33005
                     /gene="tkt"
                     /locus_tag="VC122_000104"
                     /EC_number="2.2.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013793690.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transketolase"
                     /protein_id="HEP5640063.1"
                     /translation="MTFDAIDQLAVNTVRTLSMDAIQAANSGHPGLPMGAAPMAYVLW
                     NHFMNINPKTSRNWSNRDRFILSAGHGSAMLYSLLHLAGYDLSVEDLKNFRQWGSKTP
                     GHPEVNHTDGVEATTGPLGQGIANAVGMAMAEAHLAAKFNKPGFDIVNHYTFALNGDG
                     DLMEGVSQEAASMAGHLKLGKLVLLYDSNDISLDGPTSMAFTEDVKGRFEAYGWQHIL
                     VKDGNDLEEIAAAIEAAKAETEKPTIIEVKTIIGFGAEKQGTSAVHGAPLGAEGIAFA
                     KKAYQWTHQDFEVPAEVTERFAQGLQARGEKAEQAWNDLFAVYEAEYPELAAEYQKAF
                     TNEAAQVELEAHELGSSMASRVSSQQAIQQISEQVASFWGGSADLSASNNTMVKAETD
                     FQPGHYEGRNIWFGVREFAMAAAMNGIALHGGTRVYGGTFFVFSNYLLPAVRMAALQN
                     LPTVYVMTHDSIAVGEDGPTHEPIEQLASVRSMPNFNVIRPADGNETNAAWKRAIAET
                     DRPTMLVLTRQNLPVLEGTKELAEDGLNKGAYILSEAKGDLEGILIATGSEVKLAMDT
                     QEALEAEGIHVRVVSMPSQNIFDEQSAEYKESILPAAVTKRLAIEAGSSFGWAKYVGL
                     AGKTLTIDTWGASAPGNRIFEEYGFTVANATELYKSL"
     gene            33198..33464
                     /locus_tag="VC122_000105"
     CDS             33198..33464
                     /locus_tag="VC122_000105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009881243.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bacteriocin immunity protein"
                     /protein_id="HEP5640064.1"
                     /translation="MEKKRQRLYDVIRQAYDYPENRENVALSQLLLAASNRLIKHSNP
                     LLVAYQLNQDVDNYLLDNDILLPKSLCRFKQSLEKYILEEASSV"
     gene            33498..34232
                     /locus_tag="VC122_000106"
     CDS             33498..34232
                     /locus_tag="VC122_000106"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015057994.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEP5640065.1"
                     /translation="MIEFKHVSKLYGDKEALSDLNVTINDGEIFGLIGHNGAGKTTTI
                     SILTSIIEASYGEVFVDGQLLTENREAIKKQIAYVPDSPDIFLNLTPNEYWQFLAKIY
                     GVSDEDREERLAQLTTLFELKEEVNQTIDSFSHGMRQKVIVIGALVSNPNIWILDEPL
                     TGLDPQASFDLKEMMKAHAASGHTVLFSTHVLSVAEQLCDRIGILKKGKLIFVGTIDE
                     LKEHHPDKDLESIYLELAGRKAQEEG"
     gene            34237..35865
                     /locus_tag="VC122_000107"
     CDS             34237..35865
                     /locus_tag="VC122_000107"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014635633.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEP5640066.1"
                     /translation="MNWSTIWELIKINILYSNPQSLANLKKRQENHPKENFKAYKSMM
                     RQQALMIAMFLVIYLFMFIGVDFSHYPGLFSFDVAMFFIMSTLTAFSSLYTIFYESND
                     LKLYIHLPVTSEELYIAKIVSSLGMGAVFLMPLISLLLIAYWQLLGNPLSILVAIVLF
                     LVLLVSSMVLAIYINAWVGKIIVRSRKRKLISTIMMFVSTFGAFVLIFAINISNNKRT
                     MTDGVFTDYPTIPYFKGFYDVIQAPFSTAALLNFWLPLLLILAMVYGIVTKVMPTYYR
                     EAFYISNENKAKQTKKPVNRPHQNQSLTQLLRKHHLLTLQNATLLTQTYLMPLMYVML
                     FIGPSLSRGTGFFKHISPDYFGVALLFGVSLGVMCATPTSFIGVGISLEKDNFTFIKS
                     LPITLKKFLLDKFCLLVGLQLIVPMVIYLVFGLFVLHLHPLLTIAFCLGYALSLIVQG
                     ELMYRRDYRLLDLKWQDMTQLFTRGNGQWLTMGLIFGNLIVTGVVGFGAVIIANIIQQ
                     PLLISILLSCLTLMVLGIAHLWIQKTFWKSLEKL"
     gene            35929..36750
                     /gene="proB"
                     /locus_tag="VC122_000108"
     CDS             35929..36750
                     /gene="proB"
                     /locus_tag="VC122_000108"
                     /EC_number="2.7.2.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002983578.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate 5-kinase"
                     /protein_id="HEP5640067.1"
                     /translation="MMKRQFEDVTRIVIKIGTSSLVLPTGKINLEKIDQLAFVISSLM
                     NKGKEVILVSSGAMGFGLDILKMEKRPTNLAKQQAVSSVGQVAMMSLYSQIFAHYQTN
                     VSQILLTRDVVVFPESLANVTNAFESLISLGIVPIVNENDAVSVDEMDHATKFGDNDR
                     LSAVVAGITKADLLIMLSDIDGLFDKNPTIYEDAQLRSHVAVITQEIIASAGGAGSKF
                     GTGGMLSKIQSAQMVFENKGQMVLMNGANPRDILRVLEGQPLGTWFKQIEEVTHD"
     gene            36743..37993
                     /locus_tag="VC122_000109"
     CDS             36743..37993
                     /locus_tag="VC122_000109"
                     /EC_number="1.2.1.41"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003052228.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate-5-semialdehyde dehydrogenase"
                     /protein_id="HEP5640068.1"
                     /translation="MTDMRRLGQRAKQASLLIAPLSTQIKNRFLSTLAKALVDDTQTL
                     LAANQKDLANAKEHGISDIMMDRLRLTSERIKAIAQGVQQVADLADPIGQVIKGYTNL
                     DGLKILQKRVPLGVIAMIFESRPNVSVDAFSLAFKTNNAIILRGGKDALHSNKALVKL
                     IRQSLEKSGITPDAVQLVEDPSHAVAEELMQATDYVDVLIPRGGAKLIQTVKEKAKVP
                     VIETGVGNVHIYVDAQADLDMATNIVINAKTKRPSVCNAAEGLVIHEAVAARFIPMLE
                     KAINQVQPVEWRADDKALPLFEQAVPAKAEDFETEFLDYIMSVKVVSSLEEAISWINQ
                     HTSHHSEAIITRDIKAAETFQDLVDAAAVYVNASTRFTDGFVFGLGAEIGISTQKMHA
                     RGPMGLEALTSTKFYINGDGHIRE"
     gene            38013..38234
                     /locus_tag="VC122_000110"
     CDS             38013..38234
                     /locus_tag="VC122_000110"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEP5640069.1"
                     /translation="MTNEREKEEAKVALEELLHQIQVIHLELKIVRNWGYFDLFGDGM
                     LVSWIKRRKMSKVNQLLIDLQVTLQNCRR"
     gene            38277..38441
                     /locus_tag="VC122_000111"
     CDS             38277..38441
                     /locus_tag="VC122_000111"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEP5640070.1"
                     /translation="MSWDKIFDIWLDNLLTDCCVIKEAKNFLQVVEILEAAAKSVLAK
                     LADSEQKEQD"
     gene            38465..39478
                     /gene="rsmH"
                     /locus_tag="VC122_000112"
     CDS             38465..39478
                     /gene="rsmH"
                     /locus_tag="VC122_000112"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011184838.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (cytosine(1402)-N(4))-methyltransferase
                     RsmH"
                     /protein_id="HEP5640071.1"
                     /translation="MKSFSLTFSFLNLLKYGTIKVMTKEFHHVTVLLHETVDMLDIKP
                     DGIYVDATLGGSGHSAYLLSKLGEEGHLYCFDQDQKAIDNAQVTLKSYIDKGQVTFIK
                     DNFRHLKARLTALGVDEIDGILYDLGVSSPQLDERERGFSYKQDAPLDMRMDRQSLLT
                     AYEVVNTYPFNDLVKIFFKYGEDKFSKQIARKIEQARAIKPIETTTELAELIKAAKPA
                     KELKKKGHPAKQIFQAIRIEVNDELGAADESIQDAMELLALDGRISVITFHSLEDRLT
                     KQLFKEASTVDVPKGLPLIPEDMKPKFELVSRKPILPSHSELTANKRAHSAKLRVAKK
                     IRK"
     gene            39483..39806
                     /gene="ftsL"
                     /locus_tag="VC122_000113"
     CDS             39483..39806
                     /gene="ftsL"
                     /locus_tag="VC122_000113"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012560869.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsL"
                     /protein_id="HEP5640072.1"
                     /translation="MTNEKRTQAVTNALQKRIKTFSRIEKAFYTAIIVTAITMAVSII
                     YLQSRKLQLQQEITSLNSHISDQKLELNNAKQEVNELSRRDRIIDIAGKAGLSNRNNN
                     IKKVE"
     gene            39810..42065
                     /locus_tag="VC122_000114"
     CDS             39810..42065
                     /locus_tag="VC122_000114"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002988898.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin-binding protein"
                     /protein_id="HEP5640073.1"
                     /translation="MKKWQKYVLDYVVRDRRTPVENRVRVGQNMMLLTIFIFFIFIIN
                     FMIIIGTDQKFGVSLSEGAKKVYQETVTIQAKRGTIYDRNGTAIAVDSTTYSIYAILD
                     KSFVSASDEKLYVQPSQYETVADILKKHLGMKKTDVIKQLKRKGLFQVSFGPSGSGIS
                     YSTMSTIQKAMEDAKIKGIAFTTSPGRMYPNGTFASEFIGLASLTEDKKTGVKSLVGK
                     TGLEASFDKILSGQDGVITYQKDRNGTTLLGTGKTVKKAIDGKDIYTTLSEPIQTFLE
                     TQMDVFQAKSNGQLASATLVNAKTGEILATTQRPTYNADTLKGLENTNYKWYSALHQG
                     NFEPGSTMKVMTLAAAIDDKVFNPNETFSNANGLTIADATIQDWSINEGISTGQYMNY
                     AQGFAFSSNVGMTKLEQKMGNAKWMNYLTKFRFGFPTRFGLKDEDAGIFPSDNIVTQA
                     MSAFGQGISVTQIQMLRAFTAISNNGEMLEPQFISQIYDPNTASFRTANKEIVGKPVS
                     KKAASETRQYMIGVGTDPEFGTLYSKTFGPIIKVGDLPVAVKSGTAQIGSEDGSGYQD
                     GGLTNYVYSVVAMVPADKPDFLMYVTMTKPQHFGPLFWQDVVNPVLEEAYLMQDTLTK
                     PVVSDANRQTTYKLPNFVGKNPGETSSELRRNLVQPVVLGTGSKIKKVSHQPGQTLTE
                     NQQVLILSDRFVEVPDMYGWTKSNVKTFAKWTGIDISFKGTDSGRVMKQSVDVGKSLK
                     KIKKMTITLGD"
     gene            42067..43077
                     /locus_tag="VC122_000115"
     CDS             42067..43077
                     /locus_tag="VC122_000115"
                     /EC_number="2.7.8.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011054851.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phospho-N-acetylmuramoyl-pentapeptide-
                     transferase"
                     /protein_id="HEP5640074.1"
                     /translation="MFLTLIAAIISFMVSAFTMPYFIKFYQLKKIGGQQMHEDVKQHL
                     AKAGTPTMGGTVFLLVATAVSLLVSLFSIKNTQSLALISGILSIVVIYGIIGFLDDFL
                     KIFKQINEGLTAKQKLALQLVGGLMFYFLHVSPSGISSINVFGYQLPLGIFYLFFVLF
                     WVVGFSNAVNLTDGIDGLASISVVISLVTYGVIAYVQSQFDVLLLIGAMIGALLGFFC
                     FNHKPAKVFMGDVGSLALGAMLAAISIALRQEWTLLIIGIVYVLETSSVMLQVSYFKY
                     TKKKYGEGRRIFRMTPFHHHLELGGLSGKGKKWSEWQVDAFLWGVGSLASLLVLAILY
                     VF"
     gene            43235..44578
                     /locus_tag="VC122_000116"
     CDS             43235..44578
                     /locus_tag="VC122_000116"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008535518.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase"
                     /protein_id="HEP5640075.1"
                     /translation="MSFKDYHFKQYVQQALEEIGFVNPTEVQKRLIPIVNSGRDLVGE
                     SKTGSGKTHTFLLPIFEKLDEAKAEVQVVITAPSRELATQIFDACKQIAKHFQEEIRL
                     ANYVGGTDKLRQIEKLKGSQPHIVIGTPGRIYDLVKSGDLAIHKATTFVVDEADMTMD
                     MGFLDTVDKIAASLPKSVQILVFSATIPQKLQPFLKKYLTNPVIEQIKTKTVIADTID
                     NWLVSTKGRDKNGQLLEILKTMQPYMAMLFVNTKERADDLHAFLTANGLKVAKIHGGI
                     PPRERKRIMNQVKKLDFEYIVATDLAARGIDIEGVSHVINDAIPQDLSFFVHRVGRTG
                     RNGMAGTAITLYQPSDDSDIKELEKMGIAFTPKVLKNGEFQDTYDRDRRQNREKAYQK
                     LDTEMIGLVKKKKKKVKPGYKKKIQWAVDEKRRKERRAENRAKGRAERNAKKQHF"
     gene            44773..45576
                     /locus_tag="VC122_000117"
     CDS             44773..45576
                     /locus_tag="VC122_000117"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003044798.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter permease"
                     /protein_id="HEP5640076.1"
                     /translation="MTSVFLTSGWAFYDYLISPIPHGKLFSWHAVFDAIPNIIQRLPI
                     TLGLTLSGATFGLVLALIFALVKINKVKLLYPIQAIFVSFLRGTPILVQLMLTYYGIP
                     LFLKFLNQKYGFDWNVNAIPASIFAITAFAFNEAAYASETIRAAILSVDTGEIEAAKS
                     LGMTSVQVYRRVIIPNATVVAIPTLINGLIGLTKGTSLAFNAGIVEMFAQAQILGGSD
                     YRYFERYISVALVYWSISILMEQVGRLIENKMAIKAPEQARNEKLGELR"
     gene            45576..46319
                     /locus_tag="VC122_000118"
     CDS             45576..46319
                     /locus_tag="VC122_000118"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016397450.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter ATP-binding protein"
                     /protein_id="HEP5640077.1"
                     /translation="MITIRNLSKTFSGQKVLDSLALDIEKGQVIALVGASGAGKSTFL
                     RSLNYLEKPDSGSISIGDFTVDFETITTEQVLILRRKLAMVFQQFNLFERRTALENVK
                     EGLKVVKKLSDQEATKLAQAELAKVGLADRKHHYSRHLSGGQKQRVALARALAMKPDV
                     LLLDEPTSALDPELVGEVEKSITDAAKSGQTMVLVSHDMNFVYQVADRVLFLDQGKIL
                     EQGTPEEVFRHPQKERTKEFFASYSKTYI"
     gene            46423..46647
                     /locus_tag="VC122_000119"
     CDS             46423..46647
                     /locus_tag="VC122_000119"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002983605.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4059 family protein"
                     /protein_id="HEP5640078.1"
                     /translation="MLVEIFSLYVKGLILSSLTMILICLIWTLWRAKTKRDKTLIERQ
                     AFLYEMLMVAILTIPILSFAFMSILVVIKS"
     gene            46711..47628
                     /gene="trxB"
                     /locus_tag="VC122_000120"
     CDS             46711..47628
                     /gene="trxB"
                     /locus_tag="VC122_000120"
                     /EC_number="1.8.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014635627.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin-disulfide reductase"
                     /protein_id="HEP5640079.1"
                     /translation="MYDTLIIGSGPAGMTAALYAARSNLSVAIIEQGAPGGQMNNTFD
                     IENYPGYDHISGPELAMKMYEPLEKFNVENIYGIVQKIENFGDYKCVLTEDASYEAKT
                     VIIATGAKYRVLGVPGEEYYTSRGVSYCAVCDGAFFRDQDLLVVGGGDSAVEEAIYLT
                     QFAKKVTVVHRRDQLRAQKILQDRAFANDKVDFIWDSVVKEIQGNDIKVSNVLIENVK
                     TGQVTDHAFGGVFIYVGMNPVTGMVKDLEITDQEGWIITDDHMRTSIPGIFAIGDVRQ
                     KDLRQITTAVGDGAIAGQGVYHYLESFPS"
     gene            47797..49176
                     /locus_tag="VC122_000121"
     CDS             47797..49176
                     /locus_tag="VC122_000121"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012560866.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HEP5640080.1"
                     /translation="MSIKEQTDNNELENGMVRGLENRHVQLIAIAGTIGTGLFLGAGR
                     SIALTGPSIIFVYMITGAFMFMMMRAIGEMLYYDPDQHTFINFISKYIGPGWGYFSGL
                     SYWISLIFIGMAEITAVGAYVQFWFPSWPAWLIQLVFLVLLSSINLIAVRVFGETEFW
                     FAMIKILAILALIATAIFMVLTGFETHTGHASLSNIFDHFSMFPNGKLKFFMAFQMVF
                     FAYQAIEFVGITTSETANPRKVLPKAIQEIPTRIVIFYVGALVSIMAIVPWHQLPVDE
                     SPFVMVFKLIGIKWAAALINFVVLTSAASALNSTLYSTGRHLYQIANETPNALTNRLK
                     INTLSRQGVPSRAIIASAVVVGISALINILPGVADAFSLITASSSGVYIAIYALTMIA
                     HWKYRQSKDFMADGYLMPKYKVTTPLTLAFFAFVFISLFLQESTYIGAIGATIWIIVF
                     GIYSNVKFK"
     gene            49347..50801
                     /locus_tag="VC122_000122"
     CDS             49347..50801
                     /locus_tag="VC122_000122"
                     /EC_number="6.3.4.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011888660.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nicotinate phosphoribosyltransferase"
                     /protein_id="HEP5640081.1"
                     /translation="MYKDDSLTLHTDLYQINMMQVYFEQGIHNRHAVFEVYFRKEPFN
                     NGYAVFAGLQRMVEYLEQFQFSETDLAYLEELGYPGNFLTYLKELRLELTIRSAKEGD
                     LVFANEPIVQVEGPLGQCQLVETALLNIVNFQTLIATKAARIRSVIEDEPLLEFGTRR
                     AQELDAAIWGTRAAMIGGADATSNVRAGKRFDIPVSGTHAHALVQAYGNDYDAFMAYA
                     KTHKDCVFLVDTYDTLKVGVPTAIRVAKEMGDKINFLGVRLDSGDLAYLSKTVRQQLD
                     DAGFTEAKIYASNDLDENTILNLKMQKAKIDVWGVGTKLITAYDQPALGAVYKIVSIE
                     QEDGSMRDTIKLSNNAEKVSTPGKKQVWRITSREKGKSEGDYITFTDINVNELTEIEM
                     FHPTYTYIKKTVRNFDAIPLLVDIFVKGELVYQLPTLSEIKTYAKKEFDKLWDEYKRV
                     LNPQDYPVDLARDVWQNKMTLIDNIRKDTHGKSE"
     gene            50803..51627
                     /gene="nadE"
                     /locus_tag="VC122_000123"
     CDS             50803..51627
                     /gene="nadE"
                     /locus_tag="VC122_000123"
                     /EC_number="6.3.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003052203.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ammonia-dependent NAD(+) synthetase"
                     /protein_id="HEP5640082.1"
                     /translation="MTLQEEIIRQLGVKASIDPQEEIRKTVDFLKAYLRKHSFLKTYV
                     LGISGGQDSTLAGKLAQMAIAELREETSDQAYQFIAVRLPYGVQADEADAQKALAFIA
                     PDQTLTINIKAAVDGQVEALQAAGVEISDFNKGNIKARQRMISQYAIAGQMAGAVIGT
                     DHAAENITGFFTKFGDGGADILPLFRLNKRQGKALLKVLGADAALYEKVPTADLEDQK
                     PGLADEVALGVTYQDIDDYLEGKLISKAAQATIEKWWHKGQHKRHLPITIFDDFWK"
     gene            51812..53149
                     /gene="pepC"
                     /locus_tag="VC122_000124"
     CDS             51812..53149
                     /gene="pepC"
                     /locus_tag="VC122_000124"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002988925.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminopeptidase C"
                     /protein_id="HEP5640083.1"
                     /translation="MSALTETFTEQLFAHYEANAKFSAIENAVTHNGLLKSLETRQSE
                     VDNDFVFSIDLTKDKVSNQKASGRCWMFAALNTFRHKLITEFKLENFELSQAHTFFWD
                     KYEKANWFMEQVIATADQELTSRKVKFLLDVPQQDGGQWDMVVSLFEKYGVVPKSVYP
                     ESISSSNSRELNQYLNKLLRQDAQILRDLIASGAKADQVEDRKAELLQEIFNFLAMTL
                     GLPPRHFDFAYRDKDDHYHVEKGLTPQAFYDKFVGLKLSDYVSVINAPTADKPYGKSY
                     TVEMLGNVVGSREVRYLNLDMKRFKELAIKQMQAGESVWFGSDVGQVSDRQKGILATN
                     TYDFEASMDINLSQDKAGRLDYSESLMTHAMVLTGVDLDETGKPLKWKVENSWGEKVG
                     DKGYFVASDAWMDEYTYQIVVRKEFLTADELAAYEKEPQVLAPWDPMGALAQC"
     gene            complement(53613..55778)
                     /locus_tag="VC122_000125"
     CDS             complement(53613..55778)
                     /locus_tag="VC122_000125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002994220.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin-binding protein"
                     /protein_id="HEP5640084.1"
                     /translation="MITIKNPKILKWLKYVLSAILSLIILVIIIGGLLFTFYISSAPK
                     LSEAQLKSTNSSLVYDGNNNLIADLGSEKRENVTADSIPINLVNAITSIEDKRFFNHR
                     GVDLYRIFGAAFHNLTSQTTQGGSTLDQQLIKLAYFSTNESDQTLKRKAQEVWLALQM
                     ERKYTKQEILTFYINKVYMGNGNYGMLTAAKSYYGKDLKDLSYAQLALLAGIPQAPSQ
                     YDPYLHPEAAQNRRNVVLQQMYMEKHLTKAEYETAIATPVAEGLQSLQQRSTYPKYMD
                     NYLKQVIEEVKKETNKDIFTAGLKVYTNIIPDAQQTLYNIYHSGDYVYYPDQDFQVAS
                     TIVDVTNGHVIAQLGGRNQDENVSFGTNQAVLTDRDWGSTMKPITAYAPAIESGVYTS
                     TAQSTNDSVYYWPGTTTQLFNWDLRYNGWMTIQAAIMLSRNVPAVRALEAAGLDYARS
                     FLSSLGINYPEMHYSNAISSNNSSSDKKYGASSEKMAAAYAAFANGGIYHKPRYVNKV
                     EFSDGTSKTFDEKGKRAMKETTAYMMTDMLKTVLTYGTGTAAAIPGVAQAGKTGTSNY
                     TDEELAKIGEKYGLYPDYVGTLAPDENFVGFTKRYAMAVWTGYKNRLTPVYGSSLEIA
                     SDVYRSMMTYLTNGYSEDWTMPNGLYRSGGFLYLSGTYASNTDYTNSVYNNLYSNNTT
                     TASSQTTSDDTSSSNDISNSTNTDNNSNHPSTDDKKTTH"
     gene            complement(55765..56373)
                     /gene="recU"
                     /locus_tag="VC122_000126"
     CDS             complement(55765..56373)
                     /gene="recU"
                     /locus_tag="VC122_000126"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000248791.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Holliday junction resolvase RecU"
                     /protein_id="HEP5640085.1"
                     /translation="MVNYPHNLIRQKVSSVQKQTKQNKVDFANRGMSFEAAINATNDY
                     YLSRQIAVIHKKPTPVQIVKVDYPKRSRAKIVEAYFRQASTTDYCGVYKGHYVDFEAK
                     ETRQKTAMPMKNFHLHQIEHMACVLHQKGICFVLLHFSTLKETYYLPAQALISFYQID
                     NGSKSMPIDYIRKNGFKVAFGAFPQVPYLNIIEQNFLGGDYN"
     gene            56454..56969
                     /locus_tag="VC122_000127"
     CDS             56454..56969
                     /locus_tag="VC122_000127"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002994217.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1273 domain-containing protein"
                     /protein_id="HEP5640086.1"
                     /translation="MTAILITGYRSFEIGIFDHKDPRVSIIKQAIRKDLIGYLENGVD
                     WFIFTGNLGFEQWALEVANELKEEYPLQIATIFLFETHGDRWNEKNQEVLSQFRAVDF
                     VKYYFPNYEQPTQFSQYYQFLLEKTEGAYVFYDTENETNLKYFLKKAKDMPHYQLLLL
                     TFDRLNDMSQS"
     gene            57091..57417
                     /gene="gpsB"
                     /locus_tag="VC122_000128"
     CDS             57091..57417
                     /gene="gpsB"
                     /locus_tag="VC122_000128"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008089675.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division regulator GpsB"
                     /protein_id="HEP5640087.1"
                     /translation="MTSIIYSPKDIFEQEFKTSMRGFDKKEVDEFLDNVIKDYENFNA
                     QIEALKAENEALKKAKFQARNTVSATVQQPVPQPTRVAQSATNFDILKRISKLEKEVF
                     GKQIIE"
     gene            57431..57799
                     /gene="rnpB"
                     /locus_tag="VC122_000129"
     ncRNA           57431..57799
                     /ncRNA_class="RNase_P_RNA"
                     /gene="rnpB"
                     /locus_tag="VC122_000129"
                     /product="RNase P RNA component class B"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00011"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00011"
     gene            57862..59016
                     /locus_tag="VC122_000130"
     CDS             57862..59016
                     /locus_tag="VC122_000130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002983627.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent RNA methyltransferase"
                     /protein_id="HEP5640088.1"
                     /translation="MKETFRLVATAAAGLEAVVGKEVRALGFDCQVENGKVYFEGDVE
                     AIVKTNLWLRAADRIKIIVGQFPARTFEELFQGVFALDWGNYLPLGAKFPISKAKCVK
                     SKLHNEPSVQAITKKAVVKKLQKHFHRPEGVPLQEVGSTFNIEVSILKDQATIMIDTT
                     GSSLFKRGYRVQKGGAPIKENMAAAILALSNWFPDKPLVDPTCGSGTFCIEAALIGMN
                     IAPGFNRSFAFEEWSWVDKDMVQQVRDDAEQEANYEIELDISGFDIDGRMIEIAKSNA
                     EEAGLSDVITFKQMRLQDFRTDKVNGVVISNPPYGERLLDDKAVDILYNEMGQTFAPL
                     KTWSKFILTSDELFELKYGQKADKKRKLYNGTLKVDLYQFYGERVKRHLI"
     gene            59030..60499
                     /locus_tag="VC122_000131"
     CDS             59030..60499
                     /locus_tag="VC122_000131"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014407751.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEP5640089.1"
                     /translation="MSEESKEVEVTKESQTLGLNEAKSMTIGEAVRKQSEIKAGVTKD
                     DSILDKYIKQHRDEVSSQKFDAKYTELDTASLDNFIKKQREALSKAGLVDDEPVSAES
                     AEQDSTLVEEVAEDLAPMETTAVVTGIPVEATVPVLDLDPSERGIPEPQMTKEEPKRD
                     QFLSEDSHHPAKQNTKKGWLIALFLLLLAILAVVFGWNHFLRQDSGKTTQTASKQTKT
                     SLQTDSAKKATRLKAAAKAFEKQYGTFYTDATKSKLKNSAFATLPDLEAALKALEGSA
                     YYDKAKAKVDSLKKAIAAITAVNGKFVSDVVVDGEKVSAEVKADANFDDLSSATLTTG
                     NANLDAVLQASITEGRQQLASKAEAAKAANEQAAQAQAAQGQSTSVAPSGYGLTSYDP
                     ASLQRHLSRVPYNQDVIADRANPSWAFNPGVLEKIVATSQARGYISGYQYILEPVNII
                     NGNGYYNMFKPDGTYLFSINCKTGYFVGNGKGYADALDY"
     gene            complement(60645..61127)
                     /locus_tag="VC122_000132"
     CDS             complement(60645..61127)
                     /locus_tag="VC122_000132"
                     /EC_number="4.4.1.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003044751.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S-ribosylhomocysteine lyase"
                     /protein_id="HEP5640090.1"
                     /translation="MTKEVIVESFELDHTIVKAPYVRLISEEFGPKGDRITNFDVRLV
                     QPNQNSIETAGLHTIEHLLAKLIRQRIDGMIDCSPFGCRTGFHLIMWGKHSSTDIAKV
                     IKSSLEEIATGITWEDVPGTTLESCGNYKDHSLFAAKEWAQLIIDQGISDDPFSRHVI
                     "
     gene            complement(61201..62532)
                     /locus_tag="VC122_000133"
     CDS             complement(61201..62532)
                     /locus_tag="VC122_000133"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011285054.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GntP family permease"
                     /protein_id="HEP5640091.1"
                     /translation="MEIIGSLGVLIGVIVIIYLSVKEVNIIIAAPLATSLVILFNQMD
                     PTTTLLGKEPNQFMGALSTYILNYFAIFLLGSILAKLMETSGETTSIADYILKKVGHD
                     SPYKVLVAIFLISAILTYGGISLFVVMFAVLPLARSLFKKMDLAWNLIQVPLWLGIAT
                     FTMTILPGTPAIQNVIPIQYLDTSLTAAAIPSIVGSIGCVTFGLFYMKYCLAKSMARG
                     ETYATYAFDNEIQVKTKNLPHFLASILPLLLLIIIALTGSLFGNDFFKKNIIFIALLA
                     AILTASWLFRQFIPNKIAVFNLGASSSIAPIFATASAVAFGAVVMIAPGFTFFSDLIL
                     NIPGNPLISLAVLTSSMSAITGSSSGALGIVMPNFAQYYLDQGLNPEMIHRVATIASN
                     IFTIVPQSGVFLTFLALTGLNHKNAFKETFITVSVSTFIAQVIMIAFDLFS"
     gene            complement(62603..63382)
                     /locus_tag="VC122_000134"
     CDS             complement(62603..63382)
                     /locus_tag="VC122_000134"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014407748.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-hydroxybutyrate dehydrogenase"
                     /protein_id="HEP5640092.1"
                     /translation="MTHTKEVAFITGAASGIGKQIGETFLKEGKTVVFSDINQEKLDQ
                     VVADYTKEGYDAFSVVCDVTKEEAINAAIDTVVEKYGRIDILVNNAGLQHVAMIEDFP
                     TEKFEFMIKIMLTAPFIAIKRAFPTMKAQKHGRIINMASINGVIGFAGKSAYNSAKHG
                     LIGLTKVTALEAADSGITVNAICPGYVDTPLVRGQFEDLSKTRGIPLENVLEEVLYPL
                     VPQKRLIDVQEIADYVSFLASDKAKGVTGQACILDGGYTAQ"
     gene            complement(63415..64074)
                     /locus_tag="VC122_000135"
     CDS             complement(63415..64074)
                     /locus_tag="VC122_000135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011054838.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-oxoacid CoA-transferase subunit B"
                     /protein_id="HEP5640093.1"
                     /translation="MGTSATVLSKEEIQTRIAKRVAQELEHNTLVNLGIGLPTKVANY
                     IPEGVTITLQSENGFVGLTGLTDDHYDPSIVNAGGQPVSIAPGGAFFDSSTSFGIIRG
                     GHVAATVLGALQVDKEASIANYLIPGKMVPGMGGAMDLLVGAKKVIVAMEHTNKGKAK
                     ILDKCTLPLTAQNVVNLIITEMGVFEYQDEGLCALEINPDYTFEDVQNVTEVTLIDKT
                     N"
     gene            complement(64076..64726)
                     /locus_tag="VC122_000136"
     CDS             complement(64076..64726)
                     /locus_tag="VC122_000136"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002988948.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CoA transferase subunit A"
                     /protein_id="HEP5640094.1"
                     /translation="MCKEITIKEAVAHVKDGDTIMVGGFMTNGTPEKLIDTLVEKGVK
                     DLTLICNDAGFPDKGVGKMVANKQFSTIIASHIGLNREAGRQMTEGETVIDLVPQGTL
                     AERIRSGGFGLGGFLTPTGIGTEVAKGKEVITVDGKDYLLEKPLKADIALIFANKADK
                     NGNLQYAGSENNFNHVMAANAKTTIVEAREIVDVGQMDPNFVHTPGIFVNYLVKGA"
     gene            complement(64750..65937)
                     /locus_tag="VC122_000137"
     CDS             complement(64750..65937)
                     /locus_tag="VC122_000137"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003061463.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA C-acetyltransferase"
                     /protein_id="HEP5640095.1"
                     /translation="MKNIVIVEALRTPIGAFGGSFKSVSAVELGTTVLKKILDKTQVK
                     PEMVDEVILGNVLHAGLGQNVARQVAVHSGIPEDKTAFTLDMVCGSGLKAIQLAAQSI
                     MLGDADIVIAGGVENMSQAAYVSTQHRFGQRLGNSQLIDTLVHDGLTDAFNNYHMGIT
                     AENVAQKYGISREEQDQFALESQEKAAKALENHRFADEIVPVSVPQRRKDPLIVTTDE
                     YPKVDTSLEKLQQLRPAFLPKEGTVTAGNASGINDGAALLMLMTEEKALELGLTPLVT
                     IESYASAGVAPELMGTGPIPATQKALKKAGLTISDLDLVESNEAFAAQSLAVLKDLKL
                     NPEIVNVNGGAIALGHPIGASGARILVTLIHEMKKRQVTRGLATLCIGGGQGTAVIVK
                     NNT"
     gene            66138..67034
                     /locus_tag="VC122_000138"
     CDS             66138..67034
                     /locus_tag="VC122_000138"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002988952.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HEP5640096.1"
                     /translation="MDIKQIRYFIAIVENHFNLSQAAELLYVSQPTLSMMINDFEKRE
                     NVKLFKRKRGRIIGLTYLGDNYYKDAQKVLSLYDDMFLKLHDHSKGLKGSISIGIPPL
                     ILSVVFSEVMPKLILENPGIQFNVKEIGAYQLKNELLVGNVDVAVLLSPTGIADNLVE
                     TYEIQRSELSVCLSPRHRLASKKVIQWEDLTDEQLALFDPSFMVHHLVLEACERHQVR
                     PNIILTSSSWDFMLNSTKINHNVLTICPKPITELYQFKDIKCILMERPISWRVVLTRL
                     RKKSYSEIEAYIMDDLLQSFFK"
     gene            67164..68771
                     /locus_tag="VC122_000139"
     CDS             67164..68771
                     /locus_tag="VC122_000139"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014407744.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease Y"
                     /protein_id="HEP5640097.1"
                     /translation="MVNIILLIVSALIGLILGYALISIRLKSAKEAAELTLLNAEQEA
                     VDIRGKAEVDAEHIKKTAKRESKANRKELLLEAKEEARKYREEIEQEFKSERQELKQL
                     ETRLAERSLTLDRKDENLSSKEKVLDSKEQSLTDKSKHIDERQLQVEKLEEEKKAELE
                     KVAAMTIAEAREVILMETENKLTHEIATRIRDAERDIKDRTVKTAKDLLAQAMQRLAG
                     EYVTEQTITSVHLPDDNMKGRIIGREGRNIRTLESLTGIDVIIDDTPEVVILSGFDPI
                     RREIARMTLESLIADGRIHPARIEELVEKNRLEMDNRIREYGEAAAYEIGAPNLHPDL
                     IKIMGRLQFRTSFGQNVLRHSVEVGKLAGILAGELGENVALARRAGFLHDMGKAIDRE
                     VEGSHVEIGMEFARKYKEHPVVVNTIASHHGDVEPDSVIAVLVAAADALSSARPGARN
                     ESMENYIKRLRDLEEIATSFDGVQNSFALQAGREIRIMVQPEKISDDQVVILSHKVRE
                     KIENNLDYPGNIKVTVIREMRAVDYAK"
     gene            68881..69516
                     /gene="gmk"
                     /locus_tag="VC122_000140"
     CDS             68881..69516
                     /gene="gmk"
                     /locus_tag="VC122_000140"
                     /EC_number="2.7.4.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002983649.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="guanylate kinase"
                     /protein_id="HEP5640098.1"
                     /translation="MSERGLLIVFSGPSGVGKGTVRQEIFSTPDHKFEYSVSMTTRPQ
                     RPGEVDGVDYFFRTREEFEELIKTGQMLEYAEYVGNYYGTPLTYVNETLDKGIDVFLE
                     IEVQGALQVKSKVPDGVFVFLTPPDLDELEDRLVGRGTDSQEVIAQRIERAKEEIALM
                     REYDYAVVNDEVALAAERVKRIIETEHFRVERVIGRYDKMIKITKNPFKAK"
     gene            69532..69849
                     /locus_tag="VC122_000141"
     CDS             69532..69849
                     /locus_tag="VC122_000141"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000979238.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit omega"
                     /protein_id="HEP5640099.1"
                     /translation="MMLKPSIDTLLDKVPSKYSLVILQAKRAHELEAGATPTQEFKSV
                     KSTLQALEEIESGNVVIHPDPSAKREAVRAKIEAERLAKEEEERKIKEQIAKEKEEEG
                     EKI"
     gene            69914..72298
                     /locus_tag="VC122_000142"
     CDS             69914..72298
                     /locus_tag="VC122_000142"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010922541.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="primosomal protein N'"
                     /protein_id="HEP5640100.1"
                     /translation="MEKVAHVIVDIPLMQTDKPFSYGIPKELVSLVQLGSRVHVPFGK
                     GNRLLQGFVIGFGQEDSSSLKLIQTVLDPEPVLNQEQLTLADQLRKTVFSYKITLLKA
                     MIPNLLNSNYDKVLRPESGLKKPDRDFLFEGKPSVLYSTLDREKEKIALKGIQAGHIT
                     VSYLAKDKKNLKTEKYYHVDLDALAVHPISSRAKKRQLLKDYLLTHTKEAKLATLYQA
                     FSRDVVAYFVTNHLIRIDERQIDRSEPYFDQIKPSSFLTLNEQQASAVTEIVEQIGKP
                     SKPFLIEGITGSGKTEVYLHIIEAVLKQDKTAIVLVPEISLTPQMTSRFISRFGKQVA
                     IMHSGLSDGEKFDEWRKIKTGQAKVVVGARSAIFSPLERIGAIIIDEEHESTYKQESN
                     PRYHAREVALLRAKHHQAVVVMGSATPSIESRARASKGVYHFIQLTKRANPLAKIPEV
                     TIVDFRDYIGQQAVSNFTPYLIDKIKERLVKKEQVVLMLNRRGYSSFVMCRDCGYVDK
                     CPNCDISLTLHMDTKTMNCHYCGFQKPIPITCPECHSNSIRYYGTGTQKAFDELQGVI
                     PEARILRMDVDTTRKKRSHKTILDSFGRQEADILLGTQMIAKGLDFPNVTLVGVLNAD
                     TSLNLPDFRASEKTFQLLTQVAGRAGRAHKPGEVLIQTYNPDHYAIQLAKQQDFEAFY
                     RYEMAIRRQMAYPPYYFTVGITLSHRLEASVVKKAYQVTELLKSHLSDNIKILGPTPK
                     PIARTHNLYHYQILLKYRFEDNLEETLNRILDWSQEADNRHLKLIIDCEPQQFL"
     gene            72360..73295
                     /locus_tag="VC122_000143"
     CDS             72360..73295
                     /locus_tag="VC122_000143"
                     /EC_number="2.1.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002983655.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methionyl-tRNA formyltransferase"
                     /protein_id="HEP5640101.1"
                     /translation="MIKLLFMGTPQFSATVLKGLLDNPAYEILGVVTQPDRAVGRKKD
                     IKVTPVKQLALEHGISIYQPEKLSGSQELIEIMELGADGIITAAFGQFLPTILLDSVS
                     FAINVHASLLPKYRGGAPIHYAIMNGDKEAGVTIMEMIKEMDAGDMVAKASTPILETD
                     NVGTLFEKLAIIGRDLLLDSLPAYLSGELKPIPQDHSQATFSPNISPEQEKLDWTMSD
                     QEVFNHIRGMNPWPVAHTFLEGQRLKIYEAQLAEGEGLPGQVIVKTKKSLVIAAGQGA
                     LSLLVVQPAGKPKMAIADFLNGLGRKLEVGDFIGR"
     gene            73285..74607
                     /gene="rsmB"
                     /locus_tag="VC122_000144"
     CDS             73285..74607
                     /gene="rsmB"
                     /locus_tag="VC122_000144"
                     /EC_number="2.1.1.176"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003060995.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (cytosine(967)-C(5))-methyltransferase
                     RsmB"
                     /protein_id="HEP5640102.1"
                     /translation="MADNWKISIRGKALLTLEHIFDQGAYTNIALNQQLSNKALSAKD
                     RALLTEIVYGTVSRKISLEWYLAHYVKDRDKLDKWVYYLLMLSLYQLIYLDKLPAHAI
                     VNDAVGIAKNRGNKKGAEKFVNAILRQFTSHPLPDMETIKRRNKYYSVKYSLPVWLVK
                     KLEDQFGSDRSVAIMESLFVRSKASIRVTDPLKLEEAAEALDAERSLLSATGLTKASG
                     HFAASDYFTNGDITIQDESSQLVAPTLNIDGDDIILDACSAPGGKTSHIASYLKTGKV
                     IALDLYDHKLELVKENANRLGVADNIETRKLDAREVHRHFEKDSFDKILVDAPCSGIG
                     LIRRKPDIKYNKESQDFNALQAIQLEILSSVCQTLRKGGIITYSTCTIFDEENRQVIE
                     AFLQSHPNFEQVKLNHTQADIVKDGYLIITPEQYQTDGFFIGQVRRVL"
     gene            74645..75385
                     /locus_tag="VC122_000145"
     CDS             74645..75385
                     /locus_tag="VC122_000145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003044720.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Stp1/IreP family PP2C-type Ser/Thr phosphatase"
                     /protein_id="HEP5640103.1"
                     /translation="MKISLKTDIGQKRSNNQDFINKFDNKKGITLVILADGMGGHRAG
                     NIASEMTVTDLGREWVKTDFTELSQIRDWLFETIQSENQRIYDLGQSEDFKGMGTTVE
                     AVALVESSAIYAHIGDSRIGLVHDGHYTLLTSDHSLVNELVKAGQITEEEAASHPQRN
                     IITQSIGQASPVEPDLGVRVLEPGDYLVINSDGLTNMISNDEIVTILGSKVSLDEKNQ
                     EMIDLANLRGGLDNITIALVHNESEDVE"
     gene            75382..77280
                     /gene="pknB"
                     /locus_tag="VC122_000146"
     CDS             75382..77280
                     /gene="pknB"
                     /locus_tag="VC122_000146"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011888669.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Stk1 family PASTA domain-containing Ser/Thr
                     kinase"
                     /protein_id="HEP5640104.1"
                     /translation="MIQIGKLFAGRYRILKSIGRGGMADVYLANDLILDNEDVAIKVL
                     RTNYQTDQVAVARFQREARAMAELNHPNIVAIRDIGEEDGQQFLVMEYVDGADLKRYI
                     QNHAPLSNNEVVRIMEEVLSAMTLAHQKGIVHRDLKPQNILLTKEGVVKVTDFGIAVA
                     FAETSLTQTNSMLGSVHYLSPEQARGSKATIQSDIYAMGIMLFEMLTGHIPYDGDSAV
                     TIALQHFQKPLPSIIEENHNVPQALENVVIRATAKKLSDRYGSTFEMSRDLMTALSYN
                     RSRERKIVFEDVESTKPLPKVASGPTASVKLSPPTPTVLTQESRLDQTNQTDALQPPT
                     KKKKSGRFLGTLFKILFSFFIVGVALFTYLILTKPTSVKVPNVAGTSLKVAKQELYDV
                     GLKVGKIRQIESDTVAEGNVVRTDPKAGTAKRQGSSITLYVSIGNKGFDMENYKGLDY
                     QEAMNSLIETYGVPKSKIKIERIVTNEYPENTVISQSPSAGDKFNPNGKSKITLSVAV
                     SDTITMPMVTEYSYADAVNTLTALGIDASRIKAYVPSSSSATGFVPIHSPSSKAIVSG
                     QSPYYGTSLSLSDKGEISLYLYPEETHSSSSSSSSTSSSNSSSTNDSTAPGSNTELSP
                     SETTAQTP"
     gene            77403..78095
                     /locus_tag="VC122_000147"
     CDS             77403..78095
                     /locus_tag="VC122_000147"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015057977.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell wall-active antibiotics response protein"
                     /protein_id="HEP5640105.1"
                     /translation="MKKFQFFLLIECILLAMGIMTILDNDLSSFILILVLILLALRFY
                     NQDSRNNFLLTVSLLFLFLIFMLNPYIIMAVLLGIVYIFINHFSQVKKKNRFALIRFK
                     EEKIEVNNTKHQWIGTANYESDYYCFDDINIIRISGNDTVDLTNVIVTGMDNIIVIRK
                     IFGNTTILVPIDVTVTLDVSSIYGSVDFFRCQQYDLRNESIKLKETDNQSLKKVKIIV
                     TTIAGNVEVDRR"
     gene            78092..79096
                     /locus_tag="VC122_000148"
     CDS             78092..79096
                     /locus_tag="VC122_000148"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011184815.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sensor histidine kinase"
                     /protein_id="HEP5640106.1"
                     /translation="MKKRYYALVWLYSTITILSIVFVVMDNLGITFNYLRNHLWQVER
                     LGFSILLLIVSVTLLLLLLWIIMDDNSKRNINQNLKYILNNRRLYLDETSEINTNLSR
                     LSKKMSHLTANMQKKESAYILDSQEVVKQERKRIARDLHDTVSQELFASSLILSGISM
                     SLEQLDKTQLQTQLTTVEAMLQNAQNDLRILLLHLRPTELANRTLSEGLHMILKELTD
                     KSDIEVIYKETIAQLPKTMEDNLFRIAQEFISNTLKHAKASRIEVYLNQTSTELQLKM
                     IDDGVGFDMDQVRDLSYGLKNIEDRVNDLAGNLHLISQKGKGVSMDIRLPIVKGDDDE
                     "
     gene            79089..79730
                     /locus_tag="VC122_000149"
     CDS             79089..79730
                     /locus_tag="VC122_000149"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011888671.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HEP5640107.1"
                     /translation="MSKIKVILVDDHEMVRMGLKSFLNLQADIDVVGEASNGREGVDL
                     ALALKPDVLVMDLVMPELGGVEATLEVLKKWKEAKVLVLTSYLDNEKIYPVIDAGAKG
                     YMLKTSSAAEILNAIRKVSKGELAIETEVDKKIKAHDQHPDLHEELTAREYDILHLLA
                     KGYDNQTIADELFISLKTVKTHVSNILAKLEVDDRTQAVVYAFRHHLVPQDDN"
     gene            79767..81167
                     /locus_tag="VC122_000150"
     CDS             79767..81167
                     /locus_tag="VC122_000150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015058358.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional Cof-type HAD-IIB family
                     hydrolase/peptidylprolyl isomerase"
                     /protein_id="HEP5640108.1"
                     /translation="MDAKLKYKAKKIKMVFFDIDDTLRVKDTGYMPESIQRVFKALKA
                     KGILVGIASGRARYGVPQEVQDLHADYCVKLNGAYVKDDAKTIIFQAPIPADVVVAYK
                     KWADDMGIFYGMAGRHEAVLSARNDMISNAIDNVYAQLEVCPDYNEYHDVYQMWTFED
                     KGDGLQLPAELAEHLRLVRWHDNSSDVVLKGTSKALGVSKVVDHLGLKPENILVFGDE
                     LNDLELFDYAGISIAMGVSHPLLQEKADFITKKVKEDGILYALEELGLIDKELQFPQL
                     DLPNHKGPKATIKTNHGDMTLVLFPDHAPKTVANFLGLAKEGYYDGIIFHRIIPEFMI
                     QGGDPTGTGMGGQSIYGESFEDEFSDELYNLRGALSMANAGPNTNGSQFFIVQNSKIP
                     YAKKELERGGWPAPIAASYAAIGGTPHLDRRHTVFGQLVDETSFQVLDLIAGVETGAQ
                     DKPKEDVIIETIEVFD"
     gene            81167..81544
                     /locus_tag="VC122_000151"
     CDS             81167..81544
                     /locus_tag="VC122_000151"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002983674.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S1 RNA-binding domain-containing protein"
                     /protein_id="HEP5640109.1"
                     /translation="MKIGDKLHGTITGIKPYGAFVALENGTTGLIHISEIKTGFIDDI
                     DQLLAIGNQVLVQVIDIDEYSKKPSLSMRTLAEEKQHFFHRHRYSNSRHKIGFRPLEE
                     QLPQWIEESLQFLKKNTKKSLEP"
     gene            complement(81562..82503)
                     /gene="cysK"
                     /locus_tag="VC122_000152"
     CDS             complement(81562..82503)
                     /gene="cysK"
                     /locus_tag="VC122_000152"
                     /EC_number="2.5.1.47"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011018052.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine synthase A"
                     /protein_id="HEP5640110.1"
                     /translation="MTKIYKTITELVGQTPIIKLNCLIPNEAADVYVKLEAFNPGSSV
                     KDRIALSMIEAAEAEGLISPGDVIIEPTSGNTGIGLAWVGAAKGYRVIIVMPETMSLE
                     RRQIIQAYGAELVLTPGAEGMKGAIAKAETLAIELGAWMPMQFNNPANPSIHEKTTAQ
                     EILEAFKEISLDAFVSGVGTGGTLSGVSHVLKKASPETVIYAVEAEESAVLSGQEPGP
                     HKIQGISAGFIPNTLDTKAYDQIIRVKSKDALETARLTGAKEGFLVGISSGAALYAAI
                     EVAKQLGKGKHVLTILPDNGERYLSTELYDVPVIKTK"
     gene            complement(82631..83263)
                     /locus_tag="VC122_000153"
     CDS             complement(82631..83263)
                     /locus_tag="VC122_000153"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011106658.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YigZ family protein"
                     /protein_id="HEP5640111.1"
                     /translation="MEHFKTIKASGFFEESIKKSRFICHIKRVSTEEDGKNFVNAIKK
                     EHYKANHSCFAMIIGNNSQIKRSSDDGEPSGTAGIPILSVLEKQCLTNVVVVVTRYFG
                     GIKLGTGGLIRAYSNMTATAIKRFGIIEVKQQIGLEITLSYPQYQLYSNLLDQLALTE
                     TETKFSDTIKTTLYCDTERVENLIDTLTNYYHGQISCEKIGSKVIEFPCD"
     gene            83319..84644
                     /locus_tag="VC122_000154"
     CDS             83319..84644
                     /locus_tag="VC122_000154"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009880469.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase"
                     /protein_id="HEP5640112.1"
                     /translation="MESIENSYGRLLLESQLPDSAKQLAQPLKSVVISRGKMICQRCH
                     YQLDEEARLPNGAYYCRFCLVFGRNQSDKLLYAIPPMHFPKGNYLVWSGQLTAYQEMI
                     SQQLLINMQNQKTTLVHAVTGAGKTEMIYAAIEAVINTGGWVCIASPRVDVCVEVATR
                     LSQAFSCSICLMHAESLPYQRAPIIVATTHQLLKFHKAFDLLIIDEVDAFPFVNNIQL
                     HYAASQALKEGGAKILLTATSTRTLERKVNKGEVVKLTLARRFNNRPLVIPKFIRSFN
                     LFKMIHRQKLPPKILKYLKKQRKTGYPLLIFLPTIIMAESVTAILKELLPAEQIACVS
                     SQSQNRKEDITAFRQGKKTILITTTILERGVTFPQIDVFVLGSHHRVYSSQSLVQIAG
                     RVGRSIDRPDGTLYFFHEGISKAMLLARKEIKEMNYKGYSHDLSTMSTN"
     gene            84616..85281
                     /locus_tag="VC122_000155"
     CDS             84616..85281
                     /locus_tag="VC122_000155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011054828.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ComF family protein"
                     /protein_id="HEP5640113.1"
                     /translation="MICLLCQQISQTPISITEIIFLRRISSPICQQCQKSFQKIGKSV
                     CATCCANSDIIACRDCLKWENKGYNVNHRSLYCYNAAMKAYFSQYKFQGDYLLRKVFA
                     VELADVITKYYKGYIPVPVPVSPGCFRERQFNQVSAILEAANVSYLSLFEKLDNTHQS
                     SRTKKERLLVEKSYRLLKVSNIPDKILIVDDIYTTGSTIIALRKQLAKVANSDIKSLS
                     IAR"
     gene            85361..85909
                     /gene="raiA"
                     /locus_tag="VC122_000156"
     CDS             85361..85909
                     /gene="raiA"
                     /locus_tag="VC122_000156"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002988974.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome-associated translation inhibitor RaiA"
                     /protein_id="HEP5640114.1"
                     /translation="MIKFSIRGENIEVTEAIRDYVESKLTKIEKYFAKDQEIDARVNL
                     KVYRERSSKVEVTIPLDSVTLRAEDVSQDMYGSIDLVVDKIERQIRKNKTKIAKKHRE
                     KVPTGQVFTTEFEAEEVDEIPEVQVVRTKNVTLKPMDVEEARLQMELLGHDFFIYTDS
                     EDGATNILYRREDGNLGLIEAK"
BASE COUNT        26551 a        15433 c        18347 g        25900 t
ORIGIN      
        1 gtagattaaa aggttgtgag ttgatgaatc aaaatatttc agctgccgaa tgggaagtca
       61 tgcgtgtggt gtgggccagt ggtgatatta aaagtagtga tattattact attttaagga
      121 aaaaatatca atggtcagat tctaccatta aaaccttgat tgggcgtttg gtggaaaaaa
      181 aggctttggg aacttaccgg caaggaagag cttatattta tcaagctctt ttggatgaga
      241 ctttgctaca aaaagaagca ctagcgactg ttttagacgg tatttgtcag cggcaacaca
      301 cgcatctact tcttgaacgg ctttatgact taccgatgac cttagaggaa attgaggcat
      361 tccaagagct actagaagtc aaaaaagaaa atgcagttct tgaggtgtcg tgtaattgcc
      421 tgcctgggca gtgccactgt cagaaaaagg agacgtatta atggctaaag agatattttt
      481 agtggaggga atgagttgtg cctcttgtgc tctaacaatt gaaaaaatgg tcaatcaatt
      541 gcctgaagta gaacaggctg ttgtgaattt agcgaccgag aaactcacgg ttacttatca
      601 agatgacgat ttagaaacgg ccaaagttat tcaagcggta aaagctgctg gttatggtgc
      661 acaagtcttt gatgaacatg caaaacacat gcaaacaggt cgtcgtgacg aagacctaaa
      721 ggttatttgg tctcaggtct tgtggtctgc cctattcacc attcctatat tatacctagc
      781 tatgggtcac atggtgggct tatggcttcc tccatttctg caaccagatc gctatccttt
      841 cgtttacagc tttagtcagc tactcttgac gattccagtc ttagtattaa atcgtcatta
      901 ttaccataat gggtttaaag ctttgtttaa gggatatcct aatatggact ccttggtagc
      961 tttagcaacc agctttgctt ttgcttatag tctttatgga gtggttgaaa tcagtttggg
     1021 ccaggccaac tttgtgcact ccctttattt tgaatcggtg gtagtgattt tgacactcat
     1081 tggtctagga aagtattttg aaagtcgttc taaaggccgt acttcccaag ctattcaaaa
     1141 gctcttatcc ttgaaggcta ctgttgtgag ggtatggaga aatagccaat ggcaattgtt
     1201 gccgttagaa gaggttagct atgatgacct tgtcctggtt caaccaggtg aaaaagtttc
     1261 tattgacggt attatcgtgg aaggacatag cagtttggat gaatcctttt taactgggga
     1321 aagtttgcct gttgaaaagg gagaaaaaga taatgtcttt gcgggcagct tgaatgggcc
     1381 aggcgccctc acgattaaac cagataagct tgggaatgat accttgttag cacaaattat
     1441 ccaactagtt gagaatgctc aagaaaataa agcacctatt gcagctattg ctgataaggt
     1501 ctcaggtgtc tttgttccgg tggttttggt attagctctg ttaacagggg tgttttggtt
     1561 ggtattcatg caggaaagtc ttcgttttag tttgacaaca gccatcgctg ttctagtcat
     1621 tgcttgccct tgtgcgcttg gtttagcaac gccgacagcg attatggttg gaacagggcg
     1681 tgcagctgaa aatggtattc tttttaaagg tggtgacatc ttagaacaag cccatcaggt
     1741 agatacggtt gtttttgata aaacggggac gattacggag gggaaaccaa gtcttcaaaa
     1801 gctcctaact ttttcgggag atgatcacct tatcttgcaa gaagctgctt ccttggaggc
     1861 ctattctcaa caccccttag gggaggctat tgttcgtgct gctcaggaag ctggttacga
     1921 tagtctacca gtggaagagt ttgagtcttt aaccggccta ggagttactg gtcagcttga
     1981 agggcgtcgt ttggcgattg gaaatgctgc tttaatggga attcttggta tcgatttatc
     2041 ttgtgttcag tcagtttgtg ctaaggcttc tgagaaaggc caaacccttg tatattatgc
     2101 taaagagggg cagctacgag ctcttttcag tattgctgac gctgtcaaag aagatagtca
     2161 agctaccgtc gaagctttgc atcaactggg aattcacacc atcatgctaa caggtgacca
     2221 cgatgctacc gctaaggcta ttgcctctca agtggggatt acagatgtga tcagtcaagt
     2281 cttacctgat caaaaagcag gcgtgatagc agacctccgc tctcaaggga gaaaagttgc
     2341 aatggtaggt gatggtatta atgatgctcc ggcattggct gtagcagaca ttgggatagc
     2401 tatggggtca gggacagata ttgccattga atcggcggat gtgattctga tgaaacctga
     2461 tatgctggat ttggtgaaag ctatgtcatt gagtcgagcg actatgagga ttgtcaagga
     2521 aaatttattt tgggcattca tttacaatgt cctcatgatt ccagtggcca tgggcttact
     2581 tcatcttttt ggaggcccat tattgaaccc gatgttagca ggttttgcca tgagttttag
     2641 ttcagtatca gttgttttga atgccctgcg attaaaagga aaaaccattt aaaggagaat
     2701 acaaaatgga aaaacattat caggttacag gaatgacatg tgacggttgc gcaagaacgg
     2761 tcactgaaaa attatcagct gtccccggtg tgcaatcggt ccaggtaaat ttggaaaaag
     2821 gacaggctaa ggtgacagga cgtcctttaa cgttcctact caagcgtgct ttgaaggata
     2881 ccaaatttga attacatcaa tcatcttagg ttattttggt agccttactt catctcaaaa
     2941 gaggttccag ttttttaatc tgggcttctt tttttgtggt cactcataaa gactctgacg
     3001 ttagcaggtg tgttccgagc aaaataaaaa cacctctctg gaagcaaatc acttttgcca
     3061 gaaggtgttt aaggacgatg cgctgacagg ctgtccattc ttgatgaaac ggttactcct
     3121 tgttttcgtg ggctactaat aattgtgtga actgactata acgttcgatg acgtcctcag
     3181 ttatttgcga atctgtaaca acagcatcaa tgttgtttaa atggtaaaaa atgaagaagt
     3241 cgtaggcatt gaatttttgg ttatctacca agaggtattt ttcaatagaa ttattaaaag
     3301 ctagcttttg aatctctcct tcagtttcgc tataagttgc aatatcgttt ttataaatac
     3361 cattacagct aataaaagcc ttggcaaatt ttaaggatga gatattttga ctggcaaggg
     3421 accctacaaa agctcctgta atagatcggt attcaccgcc gataagaatc aggtcaatgg
     3481 tttcgctgtc ctgaaggatg ttaaagacag ggaggctatt ggtcacaatg cgaatctgac
     3541 gattttttaa ttgctcagcg aaacattcta aagttgttcc tggaccaata aagattgttt
     3601 caccatcatt gactagttgt gtcgcatatg aagctagttc ccatttttcc tttgtttgaa
     3661 cggtttgttt ttcaatattt gaccgctctt ttttattagg catggtaatg ctttgtgcac
     3721 ctccatggat gcggatcaat ttgccggctt tttccagttc atctaagtct ctacgaactg
     3781 tcatatcaga aacgtttaaa gtctgaataa tatcattcac agtaacaata ccttgctcat
     3841 tgactatttc ggtaattttt agaagtcttt ctctttttaa catctaaggg gctcctttct
     3901 atagtttatt tacttatgtt tatcatagca tatagacctt catatttcaa taaaacaaaa
     3961 taaacaaaac aaaaacaaac atatagaaca aaaaatattg cgtaaaaatg ttgaattatg
     4021 ttgaaggtta tactataatg attttgtaaa cggttaccaa tttgttgatg atgagatgac
     4081 agttgttaaa aattgatact tatagaaagg cggtagggag tgtttcctaa tattctattt
     4141 actgaagcaa ggacgcagcc agagcttttt gatcttgttg ctagctactt ggaaaaggtt
     4201 gggtatgtga cgcaagagta tcatcaggct ttagtggagc gtgaggcggt atttccgaca
     4261 ggactcaaag tggacctcaa agatgggtca gatattttgt atgcggctat tccgcataca
     4321 gaaaccaagt attgcttggt tgatcaagtg gtctatgtta gaaatagtca agcccttacc
     4381 tttaagcaca tgattaatcc agaggaagac tgtttggtaa cggatttctt ctttattatc
     4441 aatagtcaaa acgagggtca aacgacgatt ctatccaatc tcattacctt ctttattacg
     4501 aaaggcaatc ttagctatct agccagtcta aaagatgata aacaagctat ttcaaactat
     4561 ttaatagaaa aaggagtgtt tcagcatgat taaaattcta gcagcctgcg gtgcaggggt
     4621 taactcaagc catcaaatta aagatgcgat tgaaactcag atgagtgatc gtggctatga
     4681 tgtgcattgt gatgcggtga tggtcaaaga catcacagaa gaattggtgt ctcgttatga
     4741 tatttttacg ccgattgcaa aaactgactt gggatttgag atgccaattc ccacagtaga
     4801 agcaggaccg attctctatc gtatcccaat catgagtgag cctgtttttg ctgaattaga
     4861 gcgcgtgatt aaagaaaacc atttgcagtg agttaataaa aacataagga gacaagatga
     4921 tgcaaccttt tttagacatt attaacaaga ttctgggatt tcctattcag ttgggatcag
     4981 gtgtggtcat gctgattgtc atgacaggtt tagccatgat tttcggggtt aaattcacaa
     5041 aggcactgga aggcgggatt aagttagcta tcgctcttac aggtattgga gccattattg
     5101 ggattttgac gggggctttt tcagagtctt tgcaggcctt tgttaaaaat acagggatca
     5161 gtttaaacat tattgatgtc ggttgggcac cgcttgcaac aattacctgg ggttcccctt
     5221 atacccttta tttcttgctg gtgatgctgg tggttaatat tgtcatgatt gtcatgaaaa
     5281 aaacggacac ccttgacgtc gatatttttg atatttggca tttgtctatc acaggtctat
     5341 tgattatgtg gtatgctgat cacaaccatt tgccagtcct tgtgtctctt ttgattgcta
     5401 ctgtggcagt tattcttgtt ggggtattga aaatcattaa ctctgatttg atgaaaccga
     5461 cctttgatga ccttcttggg acagggtctc aatcaccaat gacttcaact cacatgaact
     5521 acatgatgaa tccaatcatt atggtcttag ataagatttt tgacaaggtc tttccggggc
     5581 tagataaata tgactttgat gctgctaaat tgaacaaaaa aattggcttc tggggctcta
     5641 agttctttat cggaatggct ctaggttttg tgattgggat tatgggagat cctcatttca
     5701 ctgttgaatc tatcaaaaat tggtttggtc taggatttac agctggtgcc tgcttggaac
     5761 ttttctcttt gattggctct tggtttatcg cagctgttga gcctttgtca caagggatta
     5821 ccaattttgc caatgctaga atgcatgggc gtcgtttcaa tatcggtttg gactggcctt
     5881 tcattgctgg tcgcgctgaa atctgggcct gtgccaatat tcttgcccca atcatgttga
     5941 ttgaagccgt tctcctatca aaagttggca atgggatttt accattggcg gggattattg
     6001 ctatggggat gacaccagct cttcttgtgg tcacacgtgg tcgtttgatt cgcatgatta
     6061 tttttggttc cctcttattg ccattattcc tcttgtcagg aactatgatt gctccatttg
     6121 cgacagagtt ggctaaaaaa gtaggggctt ttccagcagg tacaagcgct ggttccttga
     6181 ttacgcattc aacacttgaa ggaccaatgg aaaaaatttt tggttatgtg attggtcaag
     6241 cgacaacagg tcagattgcc tcaatcatca cgctaatcat ctttgtggcc atttacctag
     6301 gtctctttgc ttggtatgct aaccaaatga aggcacgtaa cgctgagtat gcgaaaacaa
     6361 tgaaataaaa tcaggtcagg tgactgacta aaaaattagg gagaatagca gataactgcg
     6421 taaggtattt ctgccttacc ccctaacgtg aaaagagtat ttctcatgta aggcaaggcg
     6481 ctctgtggga actcacaagt gctttgtctt gaaagaaata atggaagaca ctaagactac
     6541 tggcaatcat cttgatctaa ggacaggtaa tcatgtcccc cttctctaga ttggcaagcg
     6601 ttttgggtta gaaaaggaaa agctatggct attattttag gtgcagatgc acacggaaat
     6661 gcattaaaag aattgatcaa aagttttctt caagaagaag gctatgatat tattgacgtg
     6721 actgatatta acagtgattt tattgataac acattagcgg tggcgaaagc cgttaatgaa
     6781 gcagaaggtc gtttaggcat tatggtagat gcttacggag caggtccctt tatggtagcc
     6841 acaaaattaa aaggaatggt tgctgctgaa gtgtcagatg aacgctctgc ttacatgacg
     6901 cgcggtcaca acaatgcccg catgatcaca ataggggcag agattgtagg tccagaatta
     6961 gcgaagaata tcgtcaaagg ttttgtgact ggtccctacg atggtggtcg tcatcaaatt
     7021 cgtgttgaca tgctcaataa aatggcctaa aggaggataa agtgatgaaa attgcgattg
     7081 gttgtgacca tattgtcacc aacgaaaaaa tggctgtttc tgattttttg aaatctaaag
     7141 gctatgacgt tattgactgt ggcacttatg accatacaag aacccattac cctatttttg
     7201 gaaaaaagtc ggcgaagctg tggttaatgg tcaagcagac ttgggtgttt gtatttgtgg
     7261 aactggagtt ggcattaata atgccgtgaa caaggtacca ggtattcgtt cagcccttgt
     7321 tcgagatatg acaacagcgc tttatgccaa ggaagaattg aatgccaatg tgattggctt
     7381 tggtggtaaa atcactggtg aacttcttat gtgtgatatt atagatgctt ttatcaaggc
     7441 agaatacaaa gaaactgaag agaataagaa gctaattgct aaaatagccc acttggaaag
     7501 tcatcatgcc aaccaagaag atcctgattt ttttacagaa tttttggaaa aatgggatcg
     7561 tggtgagtat cacgattagg aagaagagat gagtttaaca gttatcttaa atccttctgt
     7621 cgctatttcc tatcctttag attgtctggc tatggacacg gttaatcgtg ttgacagaac
     7681 ggcaaaaaca gctggtgata aggagctaaa tgtgaccaga atgttagcaa aagctggtca
     7741 gttatggcaa caggatttag tggtggtaaa ctgggtgact ttatcattca tcagttacaa
     7801 gaacagggca tttctaacca atttttcaaa atcaaagggg aaacgagaaa ttgtattgct
     7861 gttttacatg aaggcatgca gacaaaaatt ttggaagctg gtccctacag tgatgtggac
     7921 gaagccgaag tctctttgag ccacatgagt actattgcta agccgtttga tgtcttaact
     7981 tctcaggcag tctgcctagg agactagcta ctcactatta tcaagatttg atgactatgg
     8041 caacttttaa acaattatat cagcaacttc aagtaagaga ggtatcacat gacaatcaca
     8101 gcaaataaaa gacattactt agaaaaagtg agccatcaag gaatcatatc agctttagct
     8161 tttgatcaac gaggtgcact gaagcaaatg atggcagcac atcaagaagg agaagcgaca
     8221 gttacacaga tagaaacatt gaaagttctc gtttctgagg aattgacgcc ttatgcttca
     8281 tctattttat tagatccaga atatgggttg ctggctacta aggtaagagc taatcagaca
     8341 ggcttactgt tagcttacga aaaaacaggt tatgatgcta caaccactag ccgtttaccc
     8401 gattgcttag tggaatggtc ggtcaaacgg ttgaaagcag caggtgcaga tgctattaaa
     8461 tttttgctct attatgatgt tgatggagat gaacaaatca atttacaaaa acaagcttat
     8521 attgaacgca ttggttcaga atgtacagct gaagacatcc ctttttttct tgaacttttg
     8581 agttatgatg agagaatatc agataacaac agtgcggcat acgcaaagct taagccacat
     8641 aaggttaacg gtgctatgtc agtattttcg gataagcgtt ttggcgttga tgtgctaaaa
     8701 gtagaggttc ctgttaatat ggcttatgta gaaggtttta ctgaagggga agtccattac
     8761 agtcaagctg aggctattaa agcttttcaa gaccaagaag ctgccagcca cctcccttat
     8821 atctatttga gtgcaggcgt atctgccaaa ctttttcaag agacattata ctttgcagca
     8881 gcagcaggtg ctcaatttag cggtgttttg tgtgggcgtg ccacgtgggc aggatccgtt
     8941 ccagtttata ttactaaagg agaggacgaa gcaagaaagt ggctatgcac tgaaggcttc
     9001 caaaatattg acgagttaaa cagggtactc gaagagacag ctagcccttg gacggaaaaa
     9061 atctaaaaca accttatcct atcaagcagc tctcattgat gtgataagat aagtatgggg
     9121 ataattgttt gaatcttttg tagcaaggat gaaaatgggt tttatccttg cttttttatc
     9181 cttacatttt atctttttct acttattttt taaaggaaaa cgtggtaaac taaaagaaga
     9241 ctaaaagaca atagggtagc ttatgacaat tgatatgtgg gaaattttaa atatcatcgg
     9301 gaccattgcc tttgctcttt ctggtgctat tgttgccatg gaagaagaat ttgatatttt
     9361 agggattttt attttaggtt tcgtgacagc ctttggaggc ggagccattc gaaacaccct
     9421 aattggttta cctatagagg ccttatgggg gcaaaagcca gaattcactt gcgctttctt
     9481 tgctatggtt ttgattatgc tttttcctaa gctaatagct agaggttggg tacgggcagt
     9541 tgttttgact gatgccattg gtctggcagc ttttagtgtc caaggagcct tgcatgctgt
     9601 ccgtcttaac cagcctttaa gtgctgtcat tgtagcagct gtgttgactg gtgcaggcgg
     9661 tggtgttgtc agagatattc tggcagggcg aaaacctagt gttttgcgta gtgaaatcta
     9721 tgctgggtgg tctattcttg cagctattgt tttgcatttt aaactagcag attcaacgat
     9781 tgaatgttat gccttggttg ttttactcac gactttacgc atgattggca accgtaaaaa
     9841 atggaacctc cctaaaatca aatggaaaga ggaaagatag gatgattcaa ctaattgcaa
     9901 ttgatttgga cggcacctta ttaaaccagg acaaacagat tccaaaagag aatatcacag
     9961 ccatccaaga agcagctcag tcaggcttaa aaattgtctt atgcacaggc cggcctcagt
    10021 cagggacaag accctatttt gaccaattag gtttgacaca agaggaattc ctcattatca
    10081 ataatggctg tagtacttat agtagtcctg actggcaatt acgccattca aaaatgttga
    10141 aggtttcaga tattgaattg ctggaagagc taagccaaag ttttccagat atttacctga
    10201 ctttaactga ggaaaacgat tatttggtgc ttgaagaaga agtgccagac cttgtgcagg
    10261 aagatggaga tttggtattt accattgtca agcctgtctc cttagcagag ctgtcggaca
    10321 ctcctcgcct gatttttcaa gccatgtact taggagaaaa agctgcgcta gatgcctttg
    10381 aacgtgcggt gagaaatcag cttagtcaga gttttcatgt tgttcgtagc caagataata
    10441 ttttagaaat tctccctcag ggagtcagca aggcatcggc tctcaaagaa ttagtagaag
    10501 atttgggttt aactgctgac caagtgatgg ctattgggga tgctcccaat gacattgaga
    10561 tgttaactta tgctggttta ggagttgcta tggaaaatgc ttcagcagct atcaagccat
    10621 tagctgacaa agtcactttg actaatgata tggcgggtgt ggcccaggct atccgtcagt
    10681 ttgcacttgc agctcaaaaa gactaactat tatataaaaa aggttcccaa aaggaacctt
    10741 tttacgttaa tagaggcaag cccatagcag ccatttcttc ggcaggaacg atcatgtcat
    10801 ctgaaatcca gtttgtcaag acagaaacaa tagcattgct ccaaaaaagg cgcttatagt
    10861 gcgattgttt tttgtctttt aatttttgat aagctttcag acgtttagtg actaatcgtg
    10921 ttagaagatg gtgcaggtga tgtttgaaaa tcattttaag aagctgagct tcttttttag
    10981 cttcagtaaa aagatagagc caggcttgat aagcctgagt tttaatatca aattgtttta
    11041 gtccacgaaa aatacgacgg ataaccccta ctaacagttg ttctataatg gcttctttag
    11101 aggtataatt acgataaaaa gcgttacgag atactcctgc tttcgtcacc aattctgaaa
    11161 tcgtaatatc acctaaaggt tttgtttcta gaagattcag taaggcgatt tccatagatt
    11221 cacgtgctat gcgtcgtgtt tctttattaa attgtgttaa gttttgaagt gatctttttg
    11281 agagtgcttt atcagtcatc acaaatacct ttcaagtgtt acctctgatg agttatcagt
    11341 agcaaaaacc gacttaataa tgatataatt ttatgggaaa agtcttagtt tgtcaactaa
    11401 agttagttat caggagcctc attacctcct tgtatagctc tattggatga ctttactaac
    11461 tattgaaaag gctagtaata aaacatagga tttaatgatt agttattacc attagcctag
    11521 acatcttagc gaaggcttaa taatctggtc tttgttaccg aacacaaatt agagaaaaag
    11581 aaggataaaa aatgacttgg aaaattgtaa cagattcagg atgtgatctt agaagtttga
    11641 ctagacaatc caaagagttg cgctttgaac gtgtgccttt gaccctacag attggcacag
    11701 aaatttttag agatgatgat ggattagata tcgataatat gatgacaact atgtaccagt
    11761 cgtctaaagc aacgacatct agctgtccaa gcccagaagc ctttttgcag gcctatagag
    11821 gtgcagataa tgttatcgtg atgacaatta cagggacctt atctggcagt cataatagtg
    11881 cacgactggc taaaaatgaa cttcttgaag aaaatcctaa tgtcaatatt cacctaatcg
    11941 attctttatc agcaggtggc gagatggatt tgctcgtact ggagcttgag cggttgatca
    12001 atcttggatt atcctttgaa gaagttgtca aacagatcac agcttatcag caaaaaacac
    12061 gtttgatttt tgtgcttgcc aaggttgata atttggttaa aaatggtcga ctgagtaaat
    12121 tagtaggtaa agtaattggc ttactcaata ttagaatggt tgggaaagct agcaataaag
    12181 gaacgcttga acttcttcaa aaagcgcgtg gtcaaaaaaa agcagtttct gctttgatag
    12241 aagaaattca aaaagagggt tatgtaggcg gaaaagttta cattgctcat gctcaaaatc
    12301 caaaaatttg tgagcagatc agtgaaaaaa tcaaatcttt atatcctgat gctgtgattc
    12361 aaactggaag gacctcaggt ttgtgtagct tttatgctga agacggagga cttttaatgg
    12421 gctacgaaat ttaatgctat ctaacaaaac aaaaagctag actcttttga tttgagctag
    12481 cttttttaag atatcaggaa gactaatcac taagatagcc gtctaaaacg tttattgatt
    12541 gattttacga aaaatttttg tattactctt ttgatgattg cttgtctcct gagtcagaag
    12601 gttctgaaga ggtctgaggt ggaattggat caggatgact aggttgtggc gtaacatctg
    12661 aagctgaaga ttctgagctt gggtctaaag taggtgactg ggaattatca taagtatctg
    12721 agacgtctcc tgggaaaggt gtccagtaag agttatctgt tatttctcca ctttgtcttg
    12781 gaaattcatc aataatgggt tttgctggta aaatagcgtc taaccgagat tgtaaagcgg
    12841 cttttttacc tttgttactt aagtcgttaa ctgctttttg ggcgatatct ttaagatttt
    12901 gattgagagt ctcctcagca gctttaactg cttgagtagc tgcatcttct tgctcttgct
    12961 ttgctttggc aacgtttagc tgttcttgta gcgttttttt cttagaagat tctgctaatt
    13021 tatcaacagc tttctgggct tcttggatag cagctgcagt ggctaaacga tcagcgtcat
    13081 taactgcttt aactgctttt tttagtaaac tagcagcact tttagaatgt ttagctacct
    13141 tatcttgcgt ttgagggtgg gatccaaaaa taaggcagga gccacctatt agagtgatag
    13201 ttagaacact caacatccaa taaagcgcct ctttttgtga tttagtcatg gaatctcctt
    13261 tatgatattg tttcatcgta gccatattat acctctaatt aaacgaaatg aaaagatttg
    13321 tgcaaaaaca aatgatgtaa aatatcggaa aagcctaaca ccttatcaaa ccaataatat
    13381 gcaaatgttg acatcggtat acacagaaac gcttaaaaaa accttgtgat aaagaccgta
    13441 atacattaaa atagaataga ctaaaaaatt gagcgaggac aatattgtgt cagtaaattg
    13501 gcaagacatt gcatttcatt tctttggagg cttaggtctc tttcttttca gtatcaagta
    13561 tatgggagat ggtctgcaac aagcagcagg agataaacta aggtattaca ttgataaata
    13621 caccagtaat cccttttttg gtatcttagt cggaattgcc atgtctgcct taattcagtc
    13681 cagttcaggc gtaactgtga ttacggttgg tttggtttct gcgggtttgc taaatttgcg
    13741 acaagccatt ggaattgtta tgggggctaa tattggaacg actatcactt cgtttttaat
    13801 tggttttaaa ctaggagatt acgccttgcc catgattttt attggtgcag cctgtctttt
    13861 ctttacctct aataaaaagc tcaacaattt tgggcgtatc atttttggtg tcggaggaat
    13921 tttcttctcg ctgaacctaa tgggagatgc catggatcct ttgaaatctg tgtcagcctt
    13981 tcaaaattat ttagcgacat taggtgataa actcttccaa ggtgtcttta tcggaacggc
    14041 tttgaccatg ttgattcagt catcggcagc cattatcggg attttacagg gtctgttttc
    14101 aggtggtctt ttgactttac agggagctat tcctattttg ttaggctcaa atataggaac
    14161 ctgcattaca gctgtcttag cagctatagg ttcaaatatt gctgctaaac gtgtggcagc
    14221 agctcatgtg ttatttaatt tgatcggtac aattattttt atgattatcc tagtcccatt
    14281 cacatcattg atgttatggc tacaatcaaa gttgagttta acgcctgaga tgactatcgc
    14341 tttttctcac ggttctttta atatcactaa taccatctta ttaattcctt tcattagtct
    14401 actagctatg attgtgacta gactcattcc aggagaagat gaagtggtca aatacgaagc
    14461 cctttattta gatcgcttac ttattaccca agccccttct atcgcactag gaaatgccca
    14521 caaggaattg gttcatttag cctcttatgc gattcaagct tttgaagctt cttacagtta
    14581 tattatgaca gctgacggta aatttgggga aaaggtcaag cgttatgaaa gagcagttga
    14641 taccatcgat gaggagttaa ccacttattt ggttgatatt tctaacgaag ccctcagccc
    14701 aagtgaaaat gaagtcttgg ctggtattct tgactcttca cgtgatttgg aaagaattgg
    14761 agatcattct gagtctttag gcattctaat tgaaggcatc atatctaaac aaattggttt
    14821 ttcaatatct gctcgtcaag aattgacaga gatgtatcaa ttgacgcatt gtttaacact
    14881 ggatgccatt agagctatcg ttgatagtga tacagacttg gcgcaaacta ttgtgactcg
    14941 ccataaagaa attgaagaaa aagagcgacg attacgtaaa acccacatta agcgcttgaa
    15001 ttgtggagaa tgtacggcac aagcaggtat taattttatt gatattattt cccactatac
    15061 ccgcatcaca gatcatgcct tgaatttagc agagaaagta ctttcacacc agctttaaaa
    15121 aagagaaaac gtgaatgatc atccacgttt tcttttttga atccaaaaag tggtatatac
    15181 catttacaaa gtaaagaata aggcgtataa taaggaaaaa agggaggaaa aaagatgaca
    15241 tgctatctta aagcagattg tttttattac ccaacagagg tgaaaccggc tggctaccta
    15301 ggtcttcatg atggtgtttt tggggagtgg acagaaattg ttccggcaga tgcgcaaatc
    15361 attgactaca ccggttatca aattgcacca ggtcttgtgg atacgcatat ccatggctat
    15421 gcgggagctg atgtcatgga taattctgcc caagggattc atcaaatgag tgaaggacta
    15481 ttagcaacag gagtaacgtc ttttttaccg acgaccttaa cttctacctt tgagcagtta
    15541 gaaaaagtat cagggactat tgcttcagtt gctgatcagg taaaaggtgc taaaatacaa
    15601 ggtatctact ttgaaggtcc ttattttacc gaagaatata agggtgctca aaacccaagt
    15661 tatatgaaaa ccccacgctt ggaggaattt gatgcttggc aaaaagctgc taaagggctc
    15721 atcaaaaaaa ttgcccttgc tcctgagcgt gagggtgtca aagaatttgt gtctgcagtg
    15781 acaaaacaag gcgtaacagt ggccttaggg cattcaaatg gtacttatca agaggctaaa
    15841 gaagctgttc aagctggtgc tagtgtttgg gttcatgctt ataatggcat gcgaggattg
    15901 acccaccgtg aaccaggaat ggtgggagcg gtttataatc ttccaaatac ttacgcagag
    15961 ttgatttgtg atggccacca tgtctcacca attgcttgtg atattttgat gcagcaaaaa
    16021 gggcatgatc atgtagccat gattacggac tgtatgcgag caggtggttc accagatggt
    16081 gattacctct tgggggaatt ctctgtggtt gtggcaaatg gcactgctcg tcttaaagag
    16141 agtggaaact tggcaggatc tattttgaaa ttaaaagacg gccttagaaa tgtagtggcc
    16201 tggggaatcg caactccggc agaagccatt catatggcaa cttatgtgcc agctgtttct
    16261 gtcggtattg acgacgtatg tggtcaaatc aaagctgggc atgcggctga ctttattgtt
    16321 cttgataagg atttaacact tgttgcaacc tatttaaatg ggcaaaaagc ttttgatgcc
    16381 taagagatac aaaaggattg gagtagctat gtctggcagg ccaattcttt ttgtattttc
    16441 tttctttatg atagaataga ttaaagtaat cgcttacaga tattgataaa aggaggtttt
    16501 tgtgatggta acaacagtaa aaatgacaag tggctatgag attcccgttt taggatttgg
    16561 aacttaccaa gcagccgatg gtgaggaagc ttaccaatct accttagcag ccatcaaggc
    16621 tggctatcgt catattgata ctgcagctat ttataaaaat gaagaaagtg tgggtcgtgc
    16681 catcaaggat tctggggttc tacgagaaga cttatttatt acaactaaac tctggaatga
    16741 cgcccatagt tatgaagggg caaaagacgc tttagctgct tctcttgacc gtcttggtct
    16801 ggattatgtg gatctctatc ttatccattg gccaaaccca aaagctctgc gtaacacttg
    16861 gaaagaggca aatgcccaag catggcaata tatggaagag gcggttgaag cagggcttat
    16921 taaaagcatt ggcgtcagca attttatggt gcatcatcta gaagctcttc aagagacagc
    16981 taaaataact ccagcaatta atcagattcg attagcccca ggttgctatc aaaaggaagt
    17041 ggttgattat tgtaaggcaa atgagatttt attggaagct tggagtcctc taggacaagg
    17101 agagattttt gataatgaga ctatgcagca gttagctaat aaatatgaca aaacagtggc
    17161 acaagttgcc ttagcttggt cgctggcaga aggttttatt cccttgccaa aatcggtgca
    17221 tgatgagcgc atcaaggaaa atatggctat ttttgatgtg agcttgacac aagaagataa
    17281 aaaaacaatt cgctatctct caggaatgtc agcgattcct aaccctgata ctacaagttt
    17341 ttaaggaact acaaagattt agccataatt cgctaaatct tttttgaaaa cggtttgaca
    17401 aaggtttatc atttagctat aatctttatt agtgttttgt gaaagaaact attattgaaa
    17461 ataaggagcc gtattgtgac catcttttta gtgatgttag ctatgtttac tattcgttta
    17521 gtatttttga agaaatcgat aacaaatgaa aaagccatat tagcccaagg aggacaggaa
    17581 tttggtgctc agaacacgaa atttttgacg ttacttcaca ttatgattta tgtgtttgcg
    17641 gtgattgaag cgctgcttaa acaaattaag tttgatggca ttagtttttt aggtcttttg
    17701 ttgatgttat tatcagtagc tgttttatat gaagtgacac gtatcttagg tgacatttgg
    17761 actgtaaaat tgatgttagc aaaagaccac aaatatgtgg atcactggtt gttcaaaacg
    17821 attaaacatc ctaactactt tttaaatatc gcccctgagc tggtgggcat agccttactt
    17881 tgccatgcta aaattacggc tatgcttctt ttcccatgct acattgtagt aatatattta
    17941 cgtattcgag aagaaaacaa gctattagcg gaagtgatta ttccaaacgg tactcgaaca
    18001 aaaccttaag ctcatgatgg acaaactaat ggctttatgc taaactagac tctaggaaaa
    18061 aggatgcaag tatcttatct aaagcgagtt cggggtagtt agagccgaat ggtaggactg
    18121 cagattggcg cttccgtttg ggcagtgtga ttaagtatat ttgtcaatat tgcccaaaaa
    18181 gatactatat aaatgaagta ataaattagg gtggaaccgc gttttgacgc ccctaggtct
    18241 tatgatttag gagcatcaaa atgcggtttt ttggtgccta gacctgtaat acatgctagt
    18301 aacatcgtca ggtattttac tgatgacaaa aaaacgattg tcaatgatat accattttac
    18361 tactatcaaa ggagtacgaa atgtctaaaa aacttacctt tcaagaaatc atcttgacct
    18421 tacaacaata ttggaatgat cagggttgta tgctcatgca agcctatgac aatgaaaaag
    18481 gagctgggac catgagtcct tacacgttct tacgtgctat aggtcctgag ccatggaatg
    18541 cggcttatgt agagccatca cgtcgtcctg cagatggccg ttacggagaa aacccaaatc
    18601 gtctttacca acaccaccaa tttcaagtgg tcatgaaacc atcaccatca aatattcaag
    18661 aactttacct tgcttctttg gaaaaattgg gcatcaatcc tttagaacat gatattcgtt
    18721 ttgttgaaga taactgggaa aacccatcaa caggatcagc agggcttggt tgggaagttt
    18781 ggcttgatgg aatggaaatc acacaattta cgtatttcca acaagttggt ggccttgcga
    18841 catctccagt gacagcagaa gtaacttatg gtcttgagcg cttagcttcc tatattcaag
    18901 aagtggattc ggtctatgat attgagtggg caccaggtgt taaatatggt gaaatcttct
    18961 tacaacctga gtatgagcat tcaaaatatt ccttcgaaat atcagaccaa gacatgctgc
    19021 ttgaaaactt tgaaaagttt gaaaaagaag cctcgcgtgc cctagaagaa ggtttggttc
    19081 acccagctta tgattacgtg ctcaaatgtt ctcatacgtt taacctgctg gatgcccgtg
    19141 gcgctgtttc cgtcacagaa cgcgcaggct acattgctcg tatccgtaat ttggcacgtg
    19201 tcgttgctaa gacctttgtt gcagaacgca agaagcttgg atttccactg ttggatgaag
    19261 caacacgcgc aatcttgcta gctgaagatg acgaataagt cgatttaaag atgatctagt
    19321 tgcgagcttt gtaatcttaa gagcgagcaa gaagtgggcg accgaaggtc gccgagtcaa
    19381 ggttactccc gctgtagtga caattcggta aggttactta tcgaacgagg gagttggcga
    19441 gactttaggc tggctaacta ggaatggaac acttttgttt tctgaaatta tccactggat
    19501 aatttcactt tgcgtccccc ctacctaagg gaacaatcaa aaagatactt tttagagaaa
    19561 ggaactgaac atgggctacg gactatgtca aaaagaccaa gacttcctag gtgcaagcac
    19621 ctgcgtcgct ttgctatttt gatggtgtcc gcttaacgcc cttggtatct taattatgag
    19681 taaaaatcta ctaatcgaac ttggtctgga agaacttcca gcctacgtgg tcacaccaag
    19741 tgaaaaacaa ctgggagagc gcttggcgac tttcttaaca gaaaatcgtc tctcttttga
    19801 agatatacaa accttttcaa ctccacgccg tttggcagtg cgtgtgtctg gattagctga
    19861 tcagcaaacg gatttgactg aggactttaa agggcctgct aagaaaattg cccttgatgc
    19921 tgatgggaat ttctcaaaag ctgctcaagg atttgttcgt ggcaaaggct tgaccacaga
    19981 tgctatcgaa ttccgcgaag tcaaaggaga agaatacgtt tatgtgacca aacacgaagc
    20041 tggaaaacct gctaaggaag tgcttctagg cgttactgaa gtgttgtcag caatgacctt
    20101 cccagtcagc atgcactggg ctaacaatag ttttgaatat attcgtcctg ttcacacctt
    20161 gacagttctg ttgaatgatg aggccttgga acttgacttc ttggatattc attctggacg
    20221 cgtcagccgt ggtcaccgtt ttttaggtac tgaaaccact atcacaagtg ctgattctta
    20281 tgaggctgat ttgcgcagcc aatgcgtcat tgttgacgct aaagagcgtc aagagatgat
    20341 tgtggagcaa atcaagacgc ttgaagtgga gcagggtgtg caagttgaca ttgacgaaga
    20401 cttgcttaat gaagttttga acttggtcga gtttccaaca gctttcatgg gaaattttga
    20461 ggctaaatac cttgatgtcc cagaagaagt attggtaaca tcaatgaaaa accaccaacg
    20521 ttattttgtg gtacgtgatc aggctggtca cttgatgcca aactttgtat cagttcgtaa
    20581 tgggaatgat caagccattg aaaatgttat taaagggaac gaaaaagttt tggtagctcg
    20641 cttagaagat ggtgaattct tctggcgtga ggatcagaaa ttgcaaattg ctgatttagt
    20701 tgccaaattg accaatgtga ccttccatga aaaaatcggc tctcttgctg aacacatgga
    20761 tcgtacgcgt gtcattgccg catcacttgc taaggaagcc aacctatctg ctgaggaagt
    20821 cacagctgtt gaccgtgctg cccaaattta caaatttgac cttttaacag gtatggttgg
    20881 tgaatttgat gagctgcaag ggattatggg tgaaaaatat gctcttctag ctggtgaaga
    20941 tgctgctgtg gcaacagcaa tccgtgaaca ctacttacct gatgctgcag gaggtgcgct
    21001 acctgaaacc aaagttggcg ctgtcctggc cttggcagat aaattggata ctctcttgtc
    21061 attcttttct gttggattga ttccatctgg ctctaatgat ccttatgctc tacgtcgtgc
    21121 aacgcagggt atcgttcgta ttcttgatca ttttggatgg cgcatcccaa tggataagct
    21181 ggtagatagc ttatatgact tgtcctttga tagtttgacc tataccaata aggcggatgt
    21241 gatgaacttt atccgtgctc gtgtggataa gatgatgggc aaagccgcac caaaagacat
    21301 tcgggaagct attctagcaa gttcaacctt tgtggttcca gaaatgttag cggtagctga
    21361 agctcttgtc aaagccagcc atacggaaaa ttacaaacca gcagttgaat ctttgtcacg
    21421 tgcttttaat ttggcagaaa aagcggacgc ttctgttcag gtggacccta gcctctttga
    21481 aaatgagcaa gaaaacactt tgtttgcagc tattcaaggc ttaactctag caggcagtgc
    21541 tgcccagcag ttagaacagg tctttgccct tagcccagtg attaatgatt tctttgataa
    21601 tacaatggtt atggcagaag atcaagccct taagaacaac cgtgtagcta ttttatcaga
    21661 tttagtaagc aaagctaaga ccattgctgc ttttaaccaa ctaaacacca agtaaataga
    21721 aaggaaagag ccgttgggga cttagctaga aaagtgagtt cagtacccga gtggaaagaa
    21781 caaggccttt aaacattgct tttcacagtg agagtcaagt cagtttttta agtcttacac
    21841 ttttttccca ggctcttagt ggcttaacat ggatcctaaa aaaattgctc gtatcaatga
    21901 gctggctaaa aagaaaaaaa cggtaggctt gactggacct gaaaaagtgg aacaggcaaa
    21961 actccgtgaa gaatacattg aaggctatcg tcgctctgtc agacaccata tcgaaggcat
    22021 caaattggtg gacgaagaag gaaacgacgt gacccctgaa aaattaagac aggtgcaacg
    22081 tgaaaaaggc ttgcacggcc gttcattaga tgacccaaaa tcataagaca aaaagcagct
    22141 ctgaactgct ttttgtttat cgtgatgact gttagctttt attttaaatg gtatgatact
    22201 tcaaagtgac cacacgtttt tatattgtat agtcaaaata ttgggaaaaa ccatgtgttt
    22261 gagttataat agaagaaatc aaagaaagaa ggtagtactt atgacaatta aaaaattctc
    22321 tctactagcc atagccagcc tctctttact cagtcttgca gcctgtgata tggatgataa
    22381 agacgatcat atggataatc aaccaaagac aagtcaaact agcaagaaag ttaaactttc
    22441 agaagacaaa gccaaatcaa ttgcgttaaa agatgcttct gttacggaag ctgatgctca
    22501 aatgctatca gttacacaag ataatgaaga tggcaaagcc gtgtatgaaa tcgagtttca
    22561 aaacaaagat caagaataca gctataccat tgacgccaac tcaggggata ttgtggaaaa
    22621 atcttctgaa ccaatcaatg actaatcaag aactgtaatg aataagacag ccttaactct
    22681 tgtgtccaat tgtctttgcc tcttaggcgc ggctgtttga taccgtgttg cctttgaggc
    22741 ttagttctct ttatgaccca atgatgactc tggattagca gagtgattgt tgggtctttt
    22801 gattttcttt gataagaaag attacctcgt tagaaatgag gagacgtggt tgtcatcagt
    22861 ggatttttca agatcatcta gtgggataag ctacctatct tatcagcttt tttttagctt
    22921 taaaaattct tgataatata tttctgataa aattagctat aatagaatca aataggaaaa
    22981 cgcttactta aaggagggta atgaatgtcg caagaaaagt atatcatggc tattgatcag
    23041 ggaacaacaa gttcacgagc gattattttc aatcaaaaag gggaaaaggt tagcagtagc
    23101 caaaaagaat tccctcagat tttcccacac gctggttggg tagagcacaa tgctaatcag
    23161 atttggaatt cggttcagtc tgttattgcg ggtgctttta ttgagtccag catcaaacca
    23221 agccaaattg aagcaatcgg aattaccaac caacgtgaga ccacagtggt ttgggacaaa
    23281 aaaacgggtg tacctattta taatgctatt gtgtggcaat cacgacagac ggcaccaatt
    23341 gctgagcaac tcaaacaaga tggccataca aaaatgatcc atgaaaagac aggccttgtt
    23401 attgatgcct acttttctgc aaccaagatc cgttggattt tagatcatgt tcctggtgct
    23461 caagaacgag ctgaaaaagg cgagttactt tttggaacca ttgatacttg gctggtttgg
    23521 aaattgacag atggtgctgt tcatgtgacg gattattcca atgcggctcg taccatgctc
    23581 tataatatta aagacttaac atgggatgat gagattttag agcttttaaa tattccaaaa
    23641 gatatgcttc cagaggttaa atccaattct gaaatttatg gcaaaacagc tgcctttcat
    23701 ttttatgggg gcgaagtgcc tatttcaggt atggcgggtg accaacaggc agctttgttt
    23761 ggccaactag cttttgagcc tggtatggtg aaaaatactt atggaaccgg ttctttcatc
    23821 atcatgaata ctggtgatga gatgcagcta tcgagtaata accttttgac caccattggt
    23881 tacgggatta atgggaaggt tcactatgct ttagaaggct ctatttttat tgcaggctct
    23941 gccattcaat ggttgcgaga tggtcttaaa atgattgaga cctcaccaga gtctgaacag
    24001 ctggcactag catctaccag tgatgatgaa gtctacgttg tgcctgcctt taccggttta
    24061 ggagctcctt actgggattc taatgcgcgt ggatctgtct ttggcttgac acgtggtacc
    24121 agcaaagaag actttgtcaa agcaacctta caatctatcg cctatcaagt gcgagatgtt
    24181 attgatacca tgcaggtgga ttcaggtatt gacattcaac agctccgtgt ggatggcggt
    24241 gcagccatga ataatatgct catgcaattt caggctgata ttttaggaat tgatattgcc
    24301 cgtgctaaaa atttagaaac aacagctcta ggagcagctt tcttagcagg acttgctgtg
    24361 ggctactggg aagacatgga tgctttgaaa gagttaaatg ccacaggcca gctctttaag
    24421 gctagcatga atgaatcgcg caaagaaaaa ttatacaagg gttggaagcg cgctgttaaa
    24481 gcaactcaag ttttcaccca agaagaagat gcagatgacg atgcaaaata actagaggag
    24541 gatggaatgg aattttcaag agaaacaaga cgcttagctc tccaaaaaat gcaagaaaga
    24601 gatttagacc tactgattat tgggggaggt attacgggtg ctggtgtggc acttcaggcg
    24661 gcagctagtg gcctagatac gggtctgatt gagatgcaag attttgcgca aggaacctct
    24721 agccgttcaa ccaaattggt tcacgggggg cttcgttact tgaaacaatt tgatgtggag
    24781 gtggtttcag atacggtgtc agagcgggct gtggtgcaac aaattgcacc ccacattcca
    24841 aaaccagacc ctatgttatt acctgtttat gacgaacctg gtagtacctt tagcatgttc
    24901 cgtttgaagg tcgctatgga tttgtatgat cttttagcag gcgtgtccaa tacgccagcg
    24961 gccaacaagg tgttaaccaa agaagaagtc ttaaaacgag aaccagactt aaaacaagaa
    25021 ggcttgcttg gtggtggggt ttaccttgat ttccgtaata atgacgcaag gcttgttatt
    25081 gaaaatatca aacgagctaa tcgtgatggg gctcttattg ctagtcatgt gaaagcagaa
    25141 gatttcttgc tagatgacaa tggtaagatt attggtgtga aggcgcgtga tttgctgtca
    25201 gatcaagaaa tcattatcaa ggctaaatta gtcatcaaca ccacaggtcc atggagtgat
    25261 gagactcgtc aattctctca taagggacaa ccgattcatc aaatgcgccc tacaaaaggg
    25321 gtgcatctgg tagtggaccg tcaaaaatta ccagtgtctc aacctgtcta tgttgacaca
    25381 gggttaaatg atggtcgtat ggtctttgtc ttgccacgtg aagaaaaaac ttattttgga
    25441 acaacggaca cggactacac tggagacttg gagcacccac aagttactca agaagatgtg
    25501 gattatttgt taggcgttgt caataaccgc tttccaaatg ccaacgtgac cattgatgat
    25561 attgaaagca gttgggctgg tcttcgcccc ttgttatcag gtaatagtgc ttctgactac
    25621 aatggtggca acagcggtaa agtcagtgat gatagttttg atcacttggt tgatactgtc
    25681 aaagcctata ttaaccacga agatagccga gaagctgttg aaaaagctat taagcaggtt
    25741 gaaaccagca catctgaaaa agaattggat ccgtctgcag tgtcacgagg ttcaagtttt
    25801 gaccgtgatg agaatggact ctttaccttg gcaggtggta agattaccga ctatcgcaaa
    25861 atggctgaag gagcattgac agggattatt caaatcctca aagaagagtt tggcaaatcc
    25921 ttcaagctta tcaattcaaa aacctatcct gtttcaggag gtgaaatcaa tccagccaat
    25981 gtagattcag aaatagaagc ttatgctcaa ttaggaacac ttagtggtct ttcgatggat
    26041 gatgctaggt atttggcaaa cctttatggt tctaatgcgc caaaagtctt tgccttaact
    26101 cgtcaattaa cagcagctga agggttaagt ttagctgaaa ccttgtcctt acattatgcg
    26161 atggattatg aaatggctct taaaccgaca gattatttct tgagaagaac aaatcacctc
    26221 ttatttatgc gagatagcct agatgctttg attgacccag tgattaatga aatggctaaa
    26281 cattttgaat ggtctgatca ggaaagagtg gcacaagaag acgatcttcg tcgcgtgatt
    26341 gcagacaatg atttgagtgc cctaaaaggc cagcaggagg gttaatatgg atatctttgg
    26401 tgaatttttg ggtacagcct tattggtttt actaggaaat ggtgtggtag caggggttgt
    26461 cttacccaaa acgaaaaccc atgcttctgg ctggatcgtg attgcgactg gctggggaat
    26521 tgcagttgct gtggcagttt ttatcagtgg caaagttgcc cctgcccacc tcaatccagc
    26581 tgttagcctt gcttttgcca tgagtggaac cattgcttgg tcaacagcta ttgcctatag
    26641 ccttgcccaa ctattgggag ctatggttgg ttcaactctg gtattccttc agttcaggcc
    26701 acattatctg gctgctgaga gtcaggctga tattttaggg acatttgcga caggtcctgc
    26761 tattcgagat accagctcaa acctattgag tgaaatcttt gggacctttg tcttgatgct
    26821 tggtattttg gcatttggct tatatgatat gccagcagga ctaggaaccc tctgtgtagg
    26881 taccttggtt attgggattg ggttatcctt aggaggaacg acaggttacg ccattaaccc
    26941 agcgcgtgat ttaggacctc gtctagttca tgctattcta ccactcaaca acaagggaga
    27001 ttctgattgg tcttatgctt ggatacctgt tgtaggacca attattggag cggccttagc
    27061 agttttgctt ttccaagtca tgtcctaacc atcactaaag gggttggcta tcataggcca
    27121 gctcctttgc tttttcttta taggattagg taaaacgctt tcatatctta aaaacaactg
    27181 ctataataat actattatga cattaacgac aaagaccata catgttatcg gggcatcctt
    27241 tgctggtcta gcctttgttg ataaatataa ggacctcaat ccagattccc aaatcattct
    27301 cattgataag gaaagctgtc ccaattatat tccaaatggt atcaaccaat tattcagagg
    27361 agacattcaa gacctgtctg atgctatgtg gggaagggcc tgcctagcag cacaaataga
    27421 atctaaccac cggtttattc aagcagaagt gttagctatc gaagctccct caaacacctt
    27481 gcttctcaaa gacagtcaag gacgagtttt tgaagaaggt tatgaaacgc ttgtttgtgc
    27541 catgggtgct agcccccagt ctcattatat tgagacgtca cagaccaaca aagttttggt
    27601 aaccaaatat tatgaagaaa gccaagctag tttaaaactg attgaggcta gccaagaggt
    27661 cttggttatt ggtgccggct tgattggctt agaccttgcc tacagtttgt ccttacaagg
    27721 caaacgggtc aaactgattg aagcagccga acgtccagat ttctatcaga ctgacgcaga
    27781 actgatagcc cctgtaatgg cagaaatgtc cactcaccat gtcaccttta ttaataataa
    27841 gcgtgttacg gctatccatg agatagaggg aaaagttgtc gctcataccg agcaagggga
    27901 caccttccaa ggtgatttgg ctattttagc cattaacttt aggccaaata cacacctttt
    27961 gcaaggacaa gtggcttgtg ctctggataa gaccattttg gtcaatgaaa acttacagac
    28021 cagccaggct aatatctatg ccattggaga catggtttca ttgtattttg gtatattggg
    28081 aatggattac tatacacctt tgattaacca agcaatgaag acaggtcagg ctctggctct
    28141 gcatttagct ggatatccta ttcctccatt acagacggtc aaggtattag gaagttctca
    28201 ctttggttat tatcgcgcga gtgttggtgt gacagaagaa gaagcagagc tttatatgga
    28261 tacgtgtagc tatttgtacc aaaatgggga ttctaaaaac ctcttttggc tgaaattaat
    28321 agctagaaaa acggatggga ttttaatagg cgctcagctc ctatccaaaa ccaatgctct
    28381 tgtcatcgcc aatcaattag gtcaggctct ggctctgaaa gttacagacg gtgacttagc
    28441 atttcaagat tttctctttt tacaaggtca cagtgatttg gcttatcatc ttcatgaagc
    28501 ttgtctcaaa ttatttgaaa agaggctccg ccatgaagat tgaagactta atggataaag
    28561 aacgaaaagc tcaatatcgt ctcttggtga ccctttatca tgctaaagaa acgttaaggc
    28621 taaaagacct aatgaggcta tcaaacttgt ctaaagtgac cctattaaaa tacattgaca
    28681 acctaaatca tttgtgtcga gaacaaggtt tagcctgtca attactttta gaaaaagaca
    28741 gtctgagctt aaaagaaaat ggccagttcc attgggagga tttggtagct ctgcttctca
    28801 aagaatcggt tgcctatcag atattgacct acatgtactg ccatgaacac tttaacatca
    28861 ccaacttatc cgttgaactg atggttagtg aagctactct aaacagacaa ctagctcacc
    28921 tcaaccagct tttatcagaa tttgatctgg cgctctcgca aggcagacaa ctgggaagtg
    28981 agcttcagtg gcgttatttc tatttggaat tgttccgtca taccttgacc agacaaggca
    29041 tagacgcctt ggtcaatcag ttagacgctt cgcatttggc taccttaatt gagcggctga
    29101 ttggccagtc attatcggca gaagccttag aacaattatt gatttggttg gcgatttcgc
    29161 aagcgcgaat gtcctttcaa aaaggctaca atgatcattt tttaagagat agtgacttta
    29221 tgacatctaa tattttcttt aagcgcttag aaagcatgct tttacattac ttaagacgct
    29281 atgcccttga atttgatgct tttgaagcta agagcctctt tgtctttttg catgcttatc
    29341 ctctacttcc tattgccagc atgaagtata gtttgggttt tggaggtcct attgctgatc
    29401 acatttcaga agccttatgg cttttaaaga aggctcatgt gattatccat cagacaaagg
    29461 aggaaatcat ttatggcttg gggatatttt tttccaaagc ttactttttt aaaggagcta
    29521 tccttagcca accaaccaat agccaatacc tttatcaatt agtaggcgaa gataaaagag
    29581 cgcttttaag ggttatcata aaccatttag tgctgcaaat gggtcaggaa acggatttca
    29641 gtcagcagtt gagtgacgac attttagcat tgttggtttt ttccattgag cgccaccatg
    29701 agcctctttt agtagggctt gccttggggc aaaataaggt ggaagcagcc attgcggaat
    29761 tggccattag acgtcatttg gggcatcgta gggattttca attaatgccc tatgatcatc
    29821 aaaaagttta tgactgtctg attacctatc agactgtcta tttaccgaga caagatttac
    29881 cctattaccg gttaaaacag tatagctccc cctatgaact cactgcctta gaagcttttc
    29941 tcaaagacct tttccagcaa aaaaatacac gagaagagga actctcactc agcccagcag
    30001 ctaagtctag ttttgcacac aaaactgtgt gatacttggt cttttctaag gactgttaca
    30061 cttttataat aataaagtag gatagtctaa aggtagatga tttgaaaaag cttatcaaat
    30121 agagcttaac aaaatcgaaa atttttggag gtaagaaatg aaatttttcc tagatacagc
    30181 taatgtagcc gctattaaag cgattaatga acttggcgtt gtagatggtg tcaccaccaa
    30241 tccaaccatt atctcacgtg aaggacgtga ttttgaaaca gttatcaaag aaatctgtga
    30301 catcgtcgat ggcccaatca gtgctgaagt gacagggtta acagcagatg ctatggtaga
    30361 agaagctaga agcattgcta agtggcatga caatgtcgtc gttaaaattc caatgaccac
    30421 agaagggtta aaagcaacaa acattctttc aaaagaaggg attaaaacta atgtgacctt
    30481 gattttcaca gtaagccaag gtttaatggc aatgaaagca ggcgcaactt acattagccc
    30541 atttatcggt cgtttggaag atattggcac agatgcttat caattaatca gcgaccttcg
    30601 tgaaatcatt gacttgtacg atttccaagc agaaatcatc gcagcaagta ttcgtacaac
    30661 agcccatgtg gaagctgttg ctaaattagg cgcacacatt gcgacgattc cagacccctt
    30721 gtttgctaag atgacgcaac atcccttaac gacaaatggt ttgaaaacct ttatggaaga
    30781 ttgggcttcc tttaaaaaat aaggctaaag agtctgtggc tactgtgtca acaggctctc
    30841 ttatattcat cgtccaaaag aattcaccaa tgatgtccaa actagttttt ttctggcaaa
    30901 atagagaaaa agaatttagg gactttggag gattttctga aaaaagcgtc tatttttgtg
    30961 atacaataga caatagaaaa cgtttaattt tagttgtaat aaacagagag gttttactta
    31021 tgacatttga tgcaattgac cagttggcag taaacactgt ccgcacccta tcaatggatg
    31081 ccattcaagc ggcaaattct ggacacccag gacttccaat gggggcagca cctatggcct
    31141 atgttctttg gaatcacttc atgaacatca atcccaaaac aagccgtaat tggtcaaaca
    31201 gagaccgttt tatcctatca gcaggtcatg gaagtgccat gctttatagc ttgttacact
    31261 tagctggtta tgatttatct gtagaagatt taaagaactt ccgtcaatgg ggttctaaaa
    31321 caccaggtca cccagaagtg aatcacacag acggtgtcga agcaaccaca ggacctcttg
    31381 gtcaagggat cgcaaatgcc gttggtatgg ccatggcaga agctcaccta gcagctaaat
    31441 ttaacaaacc aggctttgac atcgttaatc actacacctt tgctttgaat ggtgacggtg
    31501 accttatgga aggggtcagc caagaagcag caagtatggc aggacattta aaacttggga
    31561 aattggtctt gctatatgat tcaaacgaca tctctcttga tggtccaacc tctatggctt
    31621 tcacagaaga tgtgaaagga cgtttcgaag cttacggttg gcaacatatc cttgtgaaag
    31681 atggaaatga tttagaagaa attgcagctg ctattgaagc agctaaagct gaaactgaga
    31741 aaccaaccat catcgaagtc aaaaccatta ttggttttgg tgccgaaaaa caaggaactt
    31801 cagctgttca cggcgctcct cttggagcag aaggcattgc ttttgccaaa aaagcttacc
    31861 aatggactca tcaagatttt gaagtgcctg ctgaagtaac agagcgcttt gctcaaggtc
    31921 ttcaagcgcg tggtgaaaaa gcagaacaag cctggaatga cttgtttgca gtttatgaag
    31981 cagaatatcc agaattagca gcagaatacc aaaaagcctt tactaacgaa gctgctcaag
    32041 tagaacttga agcgcacgaa cttggtagtt caatggcgag tcgtgtatcc agtcaacaag
    32101 ccatccaaca aatctcagaa caagtagcct ctttctgggg tggatcagca gatctttcag
    32161 catctaacaa cacaatggtt aaggctgaaa cagatttcca accaggtcac tacgaaggcc
    32221 gtaacatctg gtttggcgtt cgtgaatttg ccatggctgc agccatgaac ggtattgccc
    32281 ttcacggcgg gacacgcgtt tacggcggaa ccttttttgt cttctctaac tatcttcttc
    32341 cagctgttcg tatggcagcg ctccaaaact tgccaacagt ttatgtcatg acacatgatt
    32401 ctatcgctgt tggtgaagat gggccaacgc acgaaccaat tgaacaattg gcaagtgttc
    32461 gctcaatgcc taactttaac gttattcgtc cagcagacgg taacgaaaca aatgctgctt
    32521 ggaaacgtgc cattgctgaa acagatcgcc caaccatgct tgtattgacg cgtcaaaacc
    32581 ttccagtgct tgagggcaca aaagaattgg cagaagatgg tcttaacaaa ggagcttaca
    32641 tcttgtcaga agccaaaggc gaccttgaag gtatcttgat tgcaacaggt tcagaagtga
    32701 aactggcaat ggatactcaa gaagctcttg aagcagaagg cattcatgtg cgtgtcgtat
    32761 ctatgccatc acaaaatatc tttgacgaac agtcagcaga gtataaagaa agcatcctac
    32821 cagcagctgt gactaaacgt ctcgctatcg aagcaggttc aagctttggc tgggcaaaat
    32881 atgttggcct agcaggaaaa acattgacca ttgacacttg gggtgcttca gctccaggaa
    32941 atcgtatctt cgaagaatat ggtttcactg tggcaaatgc aactgagtta tacaaatcac
    33001 tctaggttac acatcccaaa cagagtaaat caaatgattt actctgtttt tttaaaataa
    33061 aataaccagt tctttctttt cctcaaactg cctaatacac ttcttttgat aagaagtcat
    33121 tgacaaaacg attcataaaa attataattt aattgttatt gtattagcgt aaaagtacaa
    33181 aaaataaagg agtataaatg gaaaagaaac gacagcgctt atacgatgtg attagacagg
    33241 cttatgatta tccagaaaac cgtgaaaatg tggccttgtc gcagctactt ttagcagctt
    33301 ctaatagatt aattaaacac agtaacccac ttcttgttgc ctaccaacta aaccaagatg
    33361 tggataatta cttacttgat aatgacattt tacttccaaa atcgctttgc cgttttaagc
    33421 agtcattgga aaagtatatt ttagaggaag cctcatcagt ctagggagaa aaagagaaga
    33481 aaaggaaaaa ggataccatg atagaattta aacatgtttc aaagttgtat ggcgacaaag
    33541 aagctctcag cgatttaaac gtcaccatca atgacggtga aatttttggc cttatcggac
    33601 ataatggtgc aggtaaaacc acgaccatca gcattttgac ttctattatc gaagcgagtt
    33661 atggcgaagt atttgtggat ggtcaacttc tgacagaaaa tagagaagct atcaaaaaac
    33721 aaattgctta tgtgccagat tctcctgata ttttcttgaa tctaactcca aatgagtatt
    33781 ggcagttttt agctaaaatt tatggggtgt cagatgagga ccgagaagaa cggttagcac
    33841 agctgacaac actttttgaa ctgaaggaag aagttaatca aaccattgat agtttctcac
    33901 atgggatgcg ccaaaaagtg attgtgattg gtgctcttgt atcaaatcct aacatctgga
    33961 ttttggatga accattaaca ggtttggatc ctcaggcttc ttttgacctt aaggaaatga
    34021 tgaaagctca tgcagcttct ggacatacgg tcttattttc gactcacgtg ctatcagtcg
    34081 ctgagcagtt atgtgatcgt atcgggattc taaaaaaagg gaaattgatt tttgttggaa
    34141 cgattgatga gttgaaagaa caccatcctg acaaagactt ggagagcatc taccttgaac
    34201 tcgcaggtcg taaggcgcaa gaagaggggt gatgggatga attggtctac tatttgggaa
    34261 cttatcaaga taaacattct ttattccaat ccacaaagtt tagctaacct caaaaagcgt
    34321 caggaaaacc acccgaaaga aaacttcaaa gcctataaaa gcatgatgag gcaacaggcc
    34381 ttaatgattg ccatgttttt ggtgatttac ctttttatgt ttattggtgt cgatttcagt
    34441 cattatccag gacttttctc ctttgacgtt gctatgtttt ttatcatgtc aaccttgaca
    34501 gcctttagct ctctttacac cattttttat gaaagtaatg acttaaaact ttatattcac
    34561 ttaccagtaa cgtcagaaga actctatatt gcaaaaattg tctcgtcgct agggatgggt
    34621 gctgtctttt tgatgcctct tatctctctt ctcttaattg cctattggca actattggga
    34681 aatcctttgt ctattttagt agctattgtg ctctttttag tgttactagt gagttcgatg
    34741 gtattagcta tttatatcaa tgcttgggtg ggcaaaatca ttgtaaggag tcgcaagcgg
    34801 aaactcattt caactatcat gatgtttgtc tcaacttttg gagctttcgt cttaattttt
    34861 gcgattaata ttagtaacaa caagcgtacg atgacggatg gcgtatttac tgattaccca
    34921 actattccgt atttcaaagg attctatgat gttattcagg caccgttttc tactgcggcc
    34981 ctccttaact tttggttgcc attgctactg attttagcta tggtatatgg tattgtcaca
    35041 aaagtgatgc caacttatta tcgtgaggct ttttatatta gtaacgagaa caaagccaag
    35101 caaactaaaa aaccagttaa tcgtcctcat cagaatcaat cactgacgca gttgttgcga
    35161 aaacatcatc ttttaacgtt acaaaatgca actttactga cacaaaccta tctcatgccc
    35221 ctgatgtatg tgatgctttt tatcggtcca agtttgtcac gtggcacagg tttctttaag
    35281 catatttccc cagattactt tggggtggcc ttattatttg gggtcagttt gggtgtcatg
    35341 tgtgcaacac caaccagctt tattggagta ggtatttcac ttgaaaagga taactttacc
    35401 tttattaaaa gcttaccaat aacgttaaaa aaattcttgc tggataaatt ttgcttgctc
    35461 gtcggtctgc aactgattgt gcctatggtg atttatcttg tgtttggtct ctttgtgttg
    35521 cacctgcatc ctcttctaac gattgctttt tgtcttggct acgccctgtc tctgattgtg
    35581 caaggggaat tgatgtatcg tcgtgactac cggcttctgg atttaaaatg gcaagacatg
    35641 acacaactct ttaccagagg aaatgggcag tggctaacaa tgggccttat ctttggtaat
    35701 ttaatagtca caggtgtagt aggttttgga gctgttatca ttgccaatat tatccaacaa
    35761 cctctgttga ttagtattct attgagttgt ctgacactaa tggttttagg gattgctcac
    35821 ttatggattc aaaaaacctt ctggaaaagc ttagagaagc tttaacgaag agagatcatt
    35881 attcggaaaa acagtcctct ggggctgttt ttatgctata atttgggtat gatgaaacga
    35941 caatttgaag atgtaacacg tatcgtaatt aaaattggca ctagttcatt agtgctacca
    36001 acaggtaaaa tcaatttaga aaaaatcgac caactggctt ttgtgatttc aagtttgatg
    36061 aataagggca aggaagttat tttagtatca tcgggagcga tgggatttgg actagatatt
    36121 ttgaaaatgg aaaaacgtcc gaccaatctt gctaaacaac aggcagtttc cagtgttggt
    36181 caggtggcca tgatgagtct ttattcacaa atttttgcac actaccaaac caacgtgtca
    36241 caaattttat tgacgagaga tgtggtggtt ttcccagaaa gtctagccaa tgttaccaat
    36301 gcttttgaaa gtctgattag tttgggcatt gtgccgattg ttaatgaaaa cgatgcggtt
    36361 agtgtggatg aaatggatca tgcgactaaa tttggagata atgaccgttt atctgctgtt
    36421 gttgctggta ttacaaaagc tgatttatta atcatgttgt ctgatattga tggccttttt
    36481 gataaaaacc ctactattta tgaggatgcg cagttacgca gtcatgttgc agtcattacg
    36541 caagaaatca ttgcgtcagc aggtggtgcg ggcagtaaat ttggcacagg tgggatgctt
    36601 agcaaaattc agtctgctca gatggtattt gaaaacaaag gacagatggt cttgatgaat
    36661 ggagctaatc ccagagatat tttaagagtg cttgaagggc aaccactagg cacctggttc
    36721 aagcagatag aagaggtaac acatgactga tatgagaagg ttagggcaaa gggcaaaaca
    36781 agctagtctt ttaatagcac ctttgtcgac gcaaattaaa aatcgctttt taagcacctt
    36841 agcaaaggca ttagttgacg atacacagac tctcttagcc gctaaccaaa aagatttagc
    36901 gaatgctaaa gaacatggta tttctgacat catgatggat cgcttgcgct taacttcaga
    36961 acggattaaa gcgatagcac agggagttca acaggttgct gacttagctg atccaatcgg
    37021 acaagtcatt aagggctata ctaacctcga tggccttaaa atcctccaaa agagagttcc
    37081 tctaggggtg attgcgatga tttttgagag tcgcccgaat gtttcagtgg atgcttttag
    37141 tctggcgttc aaaaccaata atgccatcat cctaagggga ggaaaagatg cccttcactc
    37201 aaataaagct ttggtgaagt tgatccgaca aagtctggaa aaatcaggga tcaccccaga
    37261 tgctgtccag ctagttgagg atccctcaca tgctgttgct gaagaattga tgcaggcaac
    37321 ggactatgtg gatgtgttga ttccacgtgg tggtgctaaa ttaattcaga ccgttaagga
    37381 aaaagcaaaa gtgcctgtca tcgaaacagg tgtggggaat gtgcatatct atgtggatgc
    37441 tcaagctgac ttagatatgg ctactaatat tgttatcaac gctaaaacaa agcgcccaag
    37501 tgtttgcaat gcagctgagg gattggtaat tcacgaagct gtagcagcaa ggtttattcc
    37561 tatgcttgaa aaagcgatca atcaggttca gccagtagaa tggcgggcag atgacaaggc
    37621 tttgccgtta tttgagcagg cagtgcctgc aaaagcagag gattttgaga ccgaattttt
    37681 agattatatc atgtctgtta aggttgtttc ctctttggaa gaagctattt cttggataaa
    37741 tcagcatact agtcaccatt ccgaagctat catcacaagg gatattaagg cagcagagac
    37801 ctttcaagat ttagtggatg ctgctgctgt ttatgtcaat gcttcaacac gatttaccga
    37861 tggttttgtt tttgggcttg gtgctgaaat tgggatttct actcaaaaaa tgcacgcccg
    37921 tgggccaatg ggcttagaag ccttgaccag cactaaattt tatattaatg gggatggaca
    37981 tatcagagaa taagctacta gaaggtccct taatgactaa tgaaagagaa aaagaagaag
    38041 caaaggttgc tttagaagaa cttttacatc agattcaggt gattcatttg gaattgaaaa
    38101 ttgtccgaaa ttggggctat tttgatcttt ttggagacgg tatgcttgtc agctggatca
    38161 aacgtcgaaa aatgagcaag gtcaaccaat tattaataga cttacaagtt actcttcaaa
    38221 actgccgaag atagctggca gacgtcgata ggctattatt agctgactta ccgaatatga
    38281 gttgggataa aatctttgat atttggttag acaacctgct gacagattgt tgtgtgatta
    38341 aagaagctaa aaatttttta caagtagttg aaattctaga ggcagcggct aagtcagtgc
    38401 ttgctaagtt ggctgatagc gaacaaaaag aacaagatta gagaagagga agattcccac
    38461 gagaatgaaa tccttttctc ttactttttc atttctaaac cttttgaagt atggtacaat
    38521 taaagttatg acaaaagaat ttcatcacgt gaccgtactc cttcacgaaa cagtggacat
    38581 gcttgacata aagcctgatg ggatttatgt tgatgcgacg ctaggtggct caggccactc
    38641 agcttatttg ttgtccaaac ttggtgaaga agggcacctc tattgttttg accaagacca
    38701 aaaggctatt gacaatgcac aagttaccct caaatcttat attgacaaag gacaggtaac
    38761 ttttattaaa gataatttta gacacctcaa agcacgttta acagcgcttg gagttgatga
    38821 aattgatggt atcttatacg accttggtgt ttccagcccg caattggatg aaagagaacg
    38881 agggttttct tataaacaag atgctccatt ggatatgcgc atggatcgtc agtcgctctt
    38941 aacagcttac gaagtggtga atacctatcc attcaatgat ttggttaaga ttttcttcaa
    39001 atatggtgaa gataaattct ccaagcagat tgctcgaaaa attgaacaag caagagctat
    39061 taagcctatt gagacaacaa cagagttggc agaattgatt aaggcagcaa agccagctaa
    39121 agagttgaag aaaaaaggcc accctgctaa acagattttt caagctattc gcattgaagt
    39181 caatgatgaa ttgggagcgg ccgatgaatc tattcaggac gctatggaat tattagccct
    39241 tgatggtcgt atctcagtca ttaccttcca ttctctggaa gatcgcctaa ccaagcagtt
    39301 gtttaaagaa gctagtacgg tggatgtgcc aaaagggctt cctctaattc ctgaagatat
    39361 gaaacctaag tttgaacttg tttcacgtaa gccgatctta cctagtcatt cagagttaac
    39421 agctaataaa agggcacact cagccaagct acgtgttgcc aaaaaaattc ggaaataagc
    39481 ctatgacaaa tgaaaaaaga actcaggcag ttacaaatgc cttgcaaaaa cgaataaaaa
    39541 ctttctctag aattgagaag gctttttata ctgctatcat tgtaacagcg attacgatgg
    39601 cagtaagtat tatttacctg caaagtcgta agttgcagtt acaacaagaa atcactagtt
    39661 taaatagtca tatttcggat caaaaactag agcttaataa tgccaagcaa gaagtaaatg
    39721 agttatctcg ccgtgatcgt attatagata ttgctggtaa agcaggctta agcaaccgta
    39781 ataataatat caagaaagtc gagtaatcta tgaaaaaatg gcaaaaatat gttttagatt
    39841 atgttgtgcg cgataggaga actccagtcg aaaatcgcgt tcgagttgga caaaatatga
    39901 tgctcttaac tatctttatt ttctttattt tcattattaa tttcatgatt attattggaa
    39961 cagatcaaaa gtttggagtt agtttgtcag aaggggcgaa gaaggtttat caagaaaccg
    40021 ttacgatcca agctaagcgt gggaccattt atgatcgaaa tggtacagct attgcagtgg
    40081 attctacgac ttatagcata tacgcaattt tggataaatc atttgtctcg gcttcagatg
    40141 aaaagttata tgtacaacct tctcagtatg aaacagtagc tgatatttta aaaaagcatc
    40201 tgggaatgaa aaaaacagat gtaattaaac agcttaagcg taaaggactt ttccaagtct
    40261 cgtttggacc gtcagggtct ggcatttcat atagtactat gtctactatt caaaaggcta
    40321 tggaagatgc caaaatcaag ggaattgctt tcacaactag tcctggtcgt atgtatccaa
    40381 acgggacatt tgcttcagaa tttataggct tggcatctct aacagaagat aaaaagacag
    40441 gtgttaagag tttagttgga aaaacaggtc tagaagcttc ttttgataaa attttatcag
    40501 gtcaagatgg tgttattact tatcaaaaag atcgaaatgg gaccacactc ttgggtacag
    40561 gtaagactgt caagaaagct attgatggca aagatattta cacaacgcta tctgagccta
    40621 tccagacctt cttagaaacc cagatggatg tttttcaagc caaatcaaat ggtcagttgg
    40681 ccagtgcaac acttgttaat gctaaaactg gtgaaatttt ggcaacaaca caacgcccta
    40741 cttataatgc tgatactctg aaaggacttg aaaatacgaa ctacaaatgg tacagtgcac
    40801 ttcatcaagg aaattttgag ccaggctcaa ccatgaaagt gatgactctg gcagcggcta
    40861 ttgatgataa agttttcaac ccaaacgaaa cctttagcaa tgctaatggt ttaacaattg
    40921 cagatgctac tattcaagac tggtctatta atgaaggcat ttccacagga cagtacatga
    40981 attatgcaca aggatttgcc ttctcaagta acgttgggat gactaaactt gaacaaaaaa
    41041 tgggtaatgc aaaatggatg aattatttga cgaagttccg ctttggtttt cctactcgtt
    41101 ttggtttaaa agatgaagac gcaggtatat ttccttctga taatatcgtg actcaagcta
    41161 tgagcgcttt tggtcaaggg atttctgtaa cccagattca aatgcttaga gcctttactg
    41221 ctatttctaa taatggtgag atgttagagc cacaatttat cagtcaaatt tatgatccta
    41281 acacagcaag ttttagaacg gcaaataaag aaattgttgg aaaacctgta tcaaaaaaag
    41341 ccgctagtga aacaagacaa tacatgattg gtgtaggaac agaccctgag tttggaacac
    41401 tctattcaaa aacatttgga ccaattatta aagtgggtga tttacctgtt gctgttaaat
    41461 caggaacagc acaaattggt tcagaagatg gaagtggtta tcaagatggt ggattgacta
    41521 actatgtcta ttcggttgtg gcaatggtgc cagctgataa accagacttt ttgatgtatg
    41581 ttactatgac taaaccacaa cattttggtc cccttttttg gcaagatgtg gttaacccag
    41641 tattggaaga agcatactta atgcaagata cactaactaa gccagtagta tcagatgcta
    41701 atcgtcaaac aacttataaa ttaccaaact ttgtaggaaa gaatcctggt gagacatcaa
    41761 gcgagttgcg tcgaaatctt gtccagccag ttgtccttgg tactggcagc aagatcaaaa
    41821 aagtatcgca tcagcccggt caaacgttaa cagaaaacca acaagttctc atattatcag
    41881 accgttttgt ggaggtacca gacatgtatg gctggacaaa atccaatgtt aaaacctttg
    41941 ctaaatggac tggaatagac atcagcttta aaggaacaga ttctggtcgt gttatgaaac
    42001 aaagtgttga tgttggtaag tccttgaaaa aaataaaaaa aatgaccatt actttaggag
    42061 attaacatgt ttttaaccct tattgcagca atcatatcct ttatggtatc tgctttcaca
    42121 atgccttatt ttatcaaatt ctatcaattg aaaaaaattg gtgggcaaca aatgcatgaa
    42181 gatgtcaagc aacatttagc taaagctgga acaccgacta tggggggaac tgtttttctt
    42241 ttagtagcta cggcagtatc gttattggtt agtctatttt cgatcaaaaa cactcaaagt
    42301 ttagctttga tttcaggcat tttatcaatt gttgtcattt acgggattat cggatttttg
    42361 gatgattttt taaaaatttt taaacaaatc aatgaaggat tgacagccaa acaaaagtta
    42421 gccttgcagt tggttggagg attgatgttc tattttttac atgttagtcc aagtggcatt
    42481 tcctctatta atgtgtttgg ttatcagctg ccgctgggaa ttttctacct attctttgtt
    42541 ttgttttggg tggttggttt ttcaaatgcc gttaatttga cagatggtat cgatggctta
    42601 gcttctatct cagtggtgat tagtctagtg acttacggcg ttattgccta cgttcagagt
    42661 caatttgatg ttttgttact gattggagca atgattggag ccttgcttgg cttcttctgc
    42721 tttaatcaca aacctgctaa agtatttatg ggagatgtag gtagtttagc ccttggagct
    42781 atgttggctg ccatttctat tgcgcttcgt caagaatgga ctctcctgat tattgggatc
    42841 gtttatgttc ttgaaacaag ttctgtgatg ttgcaagtgt cctatttcaa gtacaccaag
    42901 aaaaaatatg gagaaggtcg tcgtattttt agaatgacgc ctttccacca ccatttggaa
    42961 ttgggaggct tgtctggtaa aggcaagaag tggtcagaat ggcaggtaga tgcttttttg
    43021 tggggagtag gaagtctagc tagtttacta gttttagcta tcttatacgt gttctaaggg
    43081 ggattatcaa gaatagtcac caatagcatt ttgattaaca tcatcaataa gagagtggag
    43141 atgacccaca aatgctgatt ttgtgagtgt tgtcccactc cttttgcttg tctctaacaa
    43201 taatgatata atatggaaga aaacgaggta aaacatgtca tttaaagatt atcattttaa
    43261 gcaatacgtt cagcaagcgc tggaagaaat tggctttgtc aatccaacag aggtccagaa
    43321 gcggctgatt cctattgtta actccggtcg tgacttagtt ggggaatcaa aaacaggttc
    43381 agggaaaacc cacacctttt tgttgcccat ttttgaaaaa cttgatgaag ctaaagcaga
    43441 agtgcaagtc gttattacag cacctagccg tgaattagca actcaaattt ttgatgcttg
    43501 taaacagatt gctaagcact tccaagaaga gattcgtttg gccaactatg ttggaggcac
    43561 agacaaactc cgtcagattg aaaaactaaa aggcagccag ccgcatattg tcatcggaac
    43621 cccaggtcgt atttatgact tggtcaagtc tggtgattta gcgattcata aagcaacgac
    43681 ctttgttgtg gatgaggccg atatgaccat ggacatgggc tttttggata cggtagataa
    43741 gattgcagct tcgcttccaa aatctgttca gattttggtg ttctcagcaa ctattccaca
    43801 gaaattacaa ccctttttga aaaaatactt gaccaatcct gttattgaac aaatcaagac
    43861 aaagaccgtt atcgcagata ccattgataa ctggcttgtg tcgacaaaag ggcgtgataa
    43921 gaatggtcaa ctgttagaga ttctcaaaac catgcagcct tatatggcta tgctttttgt
    43981 caataccaag gaacgcgcag atgatttgca tgccttttta acagctaatg gcttaaaagt
    44041 ggctaagatt catgggggca ttcctccaag agaacgtaag cgcatcatga accaagtcaa
    44101 aaaactggac tttgaataca ttgttgccac agacttagca gcgcgtggga ttgacattga
    44161 aggggtcagc catgtgatca atgacgccat cccacaagac ttgtctttct ttgtccatcg
    44221 tgtaggccga acaggtcgaa atggtatggc aggaactgcc attacacttt accagccaag
    44281 tgacgactca gatatcaaag agctggaaaa aatggggatt gcctttacac cgaaggtgct
    44341 caaaaatgga gaatttcaag atacctatga ccgtgatcgc cgtcaaaacc gtgaaaaagc
    44401 ctatcaaaaa ttagacacag aaatgattgg tttggtcaaa aagaaaaaga aaaaagtcaa
    44461 accaggttac aagaaaaaaa ttcagtgggc ggttgatgaa aaacgtcgca aagaacgtcg
    44521 tgctgaaaat cgtgctaagg gccgcgctga gcgtaacgcc aaaaaacaac atttttaaga
    44581 acaaagagca agcaagtgat caattggcat ctaatcttgc tttttttaca cctgctactt
    44641 gtattgttta ccatttgtta acacaaaaag tgatcatagc ttttgcttat tgctataata
    44701 cggatttact attttacatg tgggtctaag agtgttagaa tattaagaca aacaaataca
    44761 aaggggaaat acatgacatc agtgttttta accagtggtt gggcttttta tgattacctc
    44821 atctcaccga ttccacatgg aaaacttttt agttggcatg ctgtttttga tgccattcca
    44881 aatattatcc aacggcttcc aattacgctt ggtttgacac tatcaggagc aacctttggc
    44941 ttggttttgg ctttgatttt tgcccttgtc aaaattaata aagtgaagct actataccct
    45001 attcaagcta tttttgtgag tttcttgcga ggaactccta ttttggtaca gttgatgttg
    45061 acctactacg gtattcccct ctttctaaaa tttctcaatc agaagtatgg ctttgattgg
    45121 aatgttaatg caattccggc ttctattttt gccattacag cctttgcttt taatgaagca
    45181 gcctacgcta gtgaaactat tcgggcagcc attttatcag tagatacagg tgaaattgaa
    45241 gcagcaaaaa gtctaggcat gacttctgtg caggtttacc gtcgtgtcat tattcctaat
    45301 gcaactgttg tggctattcc aaccttgatt aatgggttaa ttggcttaac taaaggaaca
    45361 tcgcttgcct ttaatgcagg gattgtcgaa atgtttgccc aggctcaaat tctaggcgga
    45421 tcagattacc gttattttga acgttatatc tctgttgctc ttgtctattg gtctatcagt
    45481 attttgatgg agcaagtagg tcgcttgatt gaaaacaaga tggccatcaa agcaccagaa
    45541 caagctagaa atgaaaagtt aggagaattg cgttgatgat tacaattaga aatttgagca
    45601 agacattctc agggcaaaaa gttttagata gtttggctct tgatattgaa aaaggtcaag
    45661 tgattgcttt agttggtgct tcaggagcag ggaaatcgac cttccttcgt agcctaaact
    45721 atttggaaaa accagattca ggatccatta gcattggtga ctttacggtt gattttgaga
    45781 ccattaccac agagcaagtc cttattttac gtcggaaact tgccatggtt tttcaacaat
    45841 ttaatctttt tgagcgtcgt acagcccttg agaatgtcaa agaaggtctt aaggtggtca
    45901 aaaaactgtc tgaccaagaa gcgactaagc tggcgcaagc agaattggct aaggttggtt
    45961 tagcagatcg aaaacatcat tactcacgtc atttatcagg gggacaaaaa caacgggtag
    46021 ctttagcaag agctctggct atgaaaccag atgttttatt actagatgag ccgacctcgg
    46081 ctcttgaccc ggaattagtt ggcgaagtgg aaaagtctat tactgatgct gctaaatccg
    46141 gacagaccat ggttttagtg agtcatgaca tgaattttgt ttaccaagtg gcagatagag
    46201 tactcttttt agatcaaggg aaaatcttgg aacaaggaac acctgaagaa gtctttagac
    46261 atcctcaaaa agaacgaacc aaagaatttt ttgcaagcta ttcaaaaaca tacatttaat
    46321 gatattcttt tgttataata aaaaggtcta ggtattcctg ggcttttctt gttatgtttt
    46381 ttagaaaaac ttatgggaaa gaaataatga ctaggtggaa atatgttagt agaaatcttt
    46441 agcctttatg taaaaggtct cattctctca agccttacta tgattttaat ttgtttaata
    46501 tggactttat ggcgagcaaa aaccaaaaga gataaaacat tgattgaaag acaagctttt
    46561 ctttatgaga tgctaatggt tgctatttta accatcccaa ttttatcgtt tgcttttatg
    46621 agtattttgg ttgtcataaa atcctaatca ttgcaagaag ggattatcat tgctaaaata
    46681 ttggcaagag tagattaaag gagaaaaatc atgtacgata cattaattat tggttcagga
    46741 ccagcaggaa tgactgccgc tttatacgct gctagaagta atttatctgt tgccattatt
    46801 gaacaagggg cacctggtgg tcagatgaac aacacttttg atatagagaa ttacccggga
    46861 tatgatcata tatctggacc agaattagct atgaaaatgt acgaaccttt agagaagttt
    46921 aacgttgaaa acatttatgg tattgttcaa aaaatagaaa attttggtga ctataagtgt
    46981 gtgttgactg aagatgctag ttatgaagct aaaacggtta ttattgcaac gggagctaaa
    47041 taccgtgtac taggtgttcc aggtgaagaa tactatacta gtcgaggagt ttcttattgt
    47101 gcagtatgtg atggtgcgtt tttccgtgac caggatcttt tggtcgttgg tggtggtgac
    47161 tcagcagttg aagaagctat ttatcttact cagtttgcta aaaaggtaac cgttgttcat
    47221 cgccgtgatc aactaagagc tcaaaaaata ctgcaagatc gtgcttttgc caatgataag
    47281 gtcgacttta tctgggattc agtcgtcaaa gaaattcaag gcaatgatat taaggtctcc
    47341 aatgtcctaa tcgaaaatgt gaaaacaggc caagtcactg accacgcttt tggaggtgtg
    47401 tttatctatg ttggtatgaa tcctgtgaca ggtatggtaa aggatctaga aattaccgat
    47461 caagaaggtt ggattatcac agatgatcat atgaggacaa gtattcctgg tatttttgcg
    47521 attggagatg ttcgtcaaaa agaccttcgc caaatcacga cagctgttgg agatggtgca
    47581 attgcagggc aaggtgttta tcactacctt gaaagtttcc cttcataagg ttaaaactcc
    47641 ttcatttttc cttgtctttc ccttaaaaaa gctaaattgc ataagacctt taagaaatat
    47701 aaagaacagt tttccttaat atctttccta gataaatgtt gaaaattttc tgaaaaaagg
    47761 atataataaa tagtatttta gtgaatggag tttagaatgt ctataaaaga acaaacagac
    47821 aataacgaac tagaaaatgg aatggtaagg ggcttagaaa atcgtcatgt gcagttaatt
    47881 gcgattgcag gaacgattgg aacaggtctc tttttagggg caggacgctc tattgcccta
    47941 acaggaccat ctattatttt tgtgtatatg attacaggtg ccttcatgtt tatgatgatg
    48001 cgtgccatcg gtgaaatgct ctattatgat ccagatcaac acacctttat caattttatt
    48061 tccaaataca ttggtccagg ctggggttat ttttcgggtc tatcctattg gatttccctt
    48121 atttttattg gaatggcaga aatcacagca gtaggtgctt atgtgcaatt ttggttccca
    48181 agctggccag cctggttgat tcagttggtt ttcttagtct tacttagttc gatcaactta
    48241 attgctgtgc gcgtttttgg ggaaacagag ttttggtttg ccatgattaa gattttagct
    48301 attttagctt tgattgcaac agctattttc atggtattga caggttttga aactcacaca
    48361 ggccatgcta gcctttccaa tatttttgac catttttcca tgttcccaaa tgggaaacta
    48421 aagttcttta tggccttcca aatggttttc tttgcttatc aagctatcga atttgtggga
    48481 attaccactt ctgagacggc taacccaaga aaagttctac caaaggctat tcaagaaatt
    48541 ccaacccgta ttgtgatctt ttatgtggga gccttggtct ctattatggc aattgtgcca
    48601 tggcatcagt taccagttga tgaatctcct tttgtgatgg tgttcaaatt gattggtatt
    48661 aaatgggcag cagccttgat taactttgtg gtcttgacat cagcagcgtc agcgcttaac
    48721 tcaacccttt attcaaccgg tcgtcatctc tatcagattg ctaatgagac tccaaacgct
    48781 ttgaccaatc gcttaaagat taatacttta tctcgacaag gggtgccaag tcgtgccatt
    48841 attgcttcag cggttgtggt tggtatctca gctttgatta atatcctacc aggagttgca
    48901 gatgcgttct cactcattac ggcctcctca tcaggtgtat atattgctat ctacgcctta
    48961 accatgattg ctcactggaa gtaccgtcaa tctaaggact ttatggcaga tggttatttg
    49021 atgccaaaat ataaagtgac aacgcctttg acccttgctt tctttgcttt tgtctttatc
    49081 tctcttttct tacaagaatc cacctatatc ggcgccattg gagcaacgat ttggattatt
    49141 gtttttggga tttatagcaa tgtcaaattt aaataataat tagcgatata gaactgaatc
    49201 cattaactct ttcttatttg aggatgtcat gatggatcag ttcttttttt actttagcga
    49261 taactcaagt tatttctaaa ccctagccaa agagtccttt ttaaaatatg ttataatagt
    49321 aacaataatt caaaggagtc ccctttatgt ataaagatga tagtttaact ctccatactg
    49381 atttgtatca gattaatatg atgcaagttt attttgagca gggcattcat aatcgtcatg
    49441 cggtgtttga agtctatttc cgcaaggaac cttttaacaa tggttatgct gtttttgccg
    49501 gtttacagcg catggtggag tatctagagc aatttcaatt ttcagaaaca gatttggctt
    49561 accttgaaga actgggttat ccaggaaact ttttgaccta ccttaaagag ttacgattag
    49621 aattgaccat ccgttcagct aaagaaggag atttggtttt tgctaatgag ccgattgtgc
    49681 aagtagaagg ccctctaggt cagtgtcagt tggtcgaaac agctctttta aacattgtta
    49741 acttccagac cttgattgct acaaaggctg ctcgtattcg ttctgtgatt gaggatgaac
    49801 cccttttgga atttggtacc cgtcgtgcac aggaactaga tgctgcgatt tggggaaccc
    49861 gcgcagcgat gattggtgga gcagatgcaa caagtaatgt gagagcaggt aaacgctttg
    49921 atatccctgt ttcaggtacg catgctcatg ctttagtgca agcctatggt aatgattatg
    49981 atgctttcat ggcttatgct aagacccata aagattgtgt atttcttgtt gacacctatg
    50041 acacacttaa agtaggtgtg ccaacagcta tccgagtagc caaagaaatg ggtgacaaga
    50101 ttaattttct aggtgttcga ttggactctg gtgacttggc ttacctctca aaaacagtcc
    50161 gtcagcagtt agacgacgct ggttttaccg aggctaagat ctatgcttct aatgacctcg
    50221 atgaaaatac catcctcaac ttaaaaatgc aaaaggccaa aattgatgtt tggggtgttg
    50281 ggaccaaact gatcacagcc tatgatcagc ctgccttagg tgctgtttat aagattgtct
    50341 ctattgagca agaagatggt agcatgcgtg atacgatcaa attatcaaat aatgcagaaa
    50401 aagtatccac accaggtaaa aaacaagttt ggcgcatcac cagtcgtgaa aaaggcaaat
    50461 cagaaggcga ctatatcacg tttactgata ttaatgttaa tgaattaact gagattgaga
    50521 tgttccatcc gacctatacc tatattaaga aaaccgtcag aaattttgat gcaattcctc
    50581 tcttagttga tatttttgtc aagggagaat tagtttacca gctaccgacc ttatctgaaa
    50641 tcaagactta tgctaaaaaa gaatttgaca agctttggga tgaatacaag cgtgtcttaa
    50701 atcctcaaga ctacccagta gacttggcac gagatgtgtg gcaaaataag atgaccttga
    50761 ttgataacat tcgtaaagat actcatggaa agagtgaatg acatgacatt acaagaagag
    50821 atcattcgac aattaggcgt caaggctagc attgatccac aagaagaaat tcgaaaaacc
    50881 gttgattttt taaaagctta tttgcgaaag cactcatttt taaaaactta tgtcttaggc
    50941 atttctggag gtcaggattc taccctcgca ggaaagctag cccagatggc cattgcagag
    51001 ttacgggagg aaacaagtga tcaagcttac caatttatag ctgttcgctt gccttacggg
    51061 gttcaggctg atgaagcaga tgcgcaaaaa gccttggctt ttattgcacc agatcagacc
    51121 cttaccatta atatcaaggc agctgtggac ggtcaagtag aggctctaca agcagcaggc
    51181 gttgagattt ctgattttaa caaaggtaat attaaagcac gtcagcgaat gataagtcaa
    51241 tatgccattg ctggccaaat ggctggtgct gttattggaa cggatcatgc ggcagaaaac
    51301 atcactggtt tctttactaa atttggtgat ggtggagcag atattcttcc cctctttagg
    51361 cttaacaaac gtcaaggaaa ggctcttttg aaagttttag gagcagatgc tgctttgtat
    51421 gaaaaagttc caactgctga tttagaagat caaaaaccag gcttggctga tgaggttgcc
    51481 ctaggggtga cctatcaaga catcgacgac tatttggaag ggaagctgat ttccaaagca
    51541 gctcaagcaa ctattgaaaa atggtggcat aaaggacaac acaagcgtca cctgccaatc
    51601 accatttttg atgatttttg gaaataagaa atgatgttca aagagtaaaa aaagaaaaca
    51661 aaaaaagtta ggttatccta acttttttct tgaagaaaat caaataaggt ctataataga
    51721 agtaatcctt tagaatagaa tggctggata ataaccatct tgccaatatt atttcaggtg
    51781 aagcttatct taatatttta ggaggacaat aatgtcagca ttaacagaga catttacaga
    51841 acaattattt gctcattacg aagccaatgc aaaattttca gctattgaga atgccgttac
    51901 gcataatggt ttgctaaaat cattagaaac ccgtcaaagt gaagtggaca atgattttgt
    51961 gttttctatt gatttaacta aagataaggt atctaaccaa aaggcatctg gtcgctgctg
    52021 gatgtttgct gccttgaaca ctttccgaca caaattaatc acagagttta aattagaaaa
    52081 ttttgaactg tcacaagccc atactttctt ttgggataag tatgaaaaag ctaactggtt
    52141 tatggagcag gtcattgcta cagctgacca agaattaacc agtcgtaaag ttaagtttct
    52201 cttggatgtt ccacaacaag atggtggcca gtgggacatg gtggtttcct tgtttgagaa
    52261 gtatggagtg gtgccaaaat ctgtttaccc agaatcaatc tcatcaagta acagtcgtga
    52321 attaaaccag tatttgaata aattattgcg ccaagatgct caaattcttc gtgacctgat
    52381 tgcatcaggt gctaaggcag accaagttga ggatcgaaaa gcagagcttc ttcaggaaat
    52441 ttttaacttc ttggccatga cccttggttt accacctcgt cattttgatt ttgcctaccg
    52501 tgataaagat gaccactatc atgttgagaa aggtttgaca ccgcaagcgt tttacgacaa
    52561 atttgttggc ttaaagcttt ctgattatgt gtctgttatt aatgcgccaa ctgctgataa
    52621 gccttatggc aaatcataca ctgttgagat gctaggaaat gtggtcggta gtcgtgaggt
    52681 tcgctacctc aaccttgata tgaaacgttt caaagaacta gctatcaagc agatgcaggc
    52741 tggtgagtcc gtttggtttg gctctgatgt gggtcaagtt tcagatcgtc aaaaagggat
    52801 tttggcaact aatacttacg attttgaagc tagtatggat atcaatctta gtcaagataa
    52861 ggctggacgt ttggattata gtgaaagttt gatgacacat gctatggttt taacaggtgt
    52921 tgacttagat gaaacaggaa aaccgttaaa atggaaggtt gaaaattcat ggggtgagaa
    52981 ggttggtgac aaaggttact ttgttgcttc agatgcttgg atggatgaat atacctatca
    53041 aattgttgtc cgtaaagaat tcttaacagc tgacgagtta gcggcttatg aaaaagagcc
    53101 acaagtacta gctccttggg atccaatggg tgctttagca caatgctaat tcctttcttt
    53161 gcgactgtat gaaatctaaa ggctggacaa aacgaacagc aaacggattg gactccgcat
    53221 aagaaagtga acatgaaaaa aattattatg ctaagaaaat gacatatgaa aaaacacgtc
    53281 ttcggtatat ggaaacgtgt tttttcatgt ttactctctt atggcattag ttgctattta
    53341 taacaatttt aaataagtct attttgtttt aggttaacgt ttgaaaaatc agttgctaat
    53401 tcccttaacg ttgtaccttt tctttaatac gattaaagaa ttctgttttt tgttttatat
    53461 tgtatgtggt aagtcatttg tgtcatataa aaacgagtcc tcacagaacg tggactcgct
    53521 ttttatttag attatcactt aaggtagata ttaaaagtaa ctaaatggat atgtaataaa
    53581 aggtaggtac agacaataag tagagttaga gattaatgag ttgtcttttt atcatcggta
    53641 gatggatgat tgctattgtt gtctgtattg gttgaattac ttatatcatt gctactacta
    53701 gtatcatctg aagtcgtttg gctagaagct gttgtcgtgt tattgctgta aagattgttg
    53761 taaaccgaat tagtatagtc ggtgttgctc gcataggttc cgcttaagta gaggaatcca
    53821 ccactgcgat aaagaccatt tggcatcgtc caatcttcac tgtaaccatt tgttaagtaa
    53881 gtcatcatgc tacgataaac gtcagatgca atctctagac ttgatccgta tactggggtc
    53941 aagcggtttt tgtaacctgt ccaaacagcc atggcgtagc gcttagtaaa gccaacaaag
    54001 ttttcgtctg gcgctaatgt accaacataa tctggataaa ggccgtattt ttcaccaatt
    54061 ttagctagtt cctcatcagt gtagttagaa gtccctgttt taccagcttg cgcaacacca
    54121 ggaatggcag cagcagtacc tgtaccataa gtgagaacag tttttaacat atctgtcatc
    54181 atataggccg tggtttcttt catggcacgt tttccttttt catcaaaagt tttacttgta
    54241 ccatcactaa attccacttt attgacatac cttggtttat gataaatacc accatttgca
    54301 aaagcagcgt atgcagcggc cattttttca ctacttgcac catatttttt atctgagcta
    54361 ctgttattac ttgagatagc gtttgagtag tgcatttcgg gatagttaat acctaaactg
    54421 cttaagaaag atcgagcata gtcaagtcct gcggcttcca gtgctcggac tgctgggaca
    54481 tttcgcgata gcataatagc agcttggatt gtcatccatc cgttatatct aaggtcccag
    54541 ttaaacaatt gggtagtggt tccaggccaa taatagactg agtcattagt cgactgagca
    54601 gtagaagtat aaacaccaga ttcaatagca ggagcatagg ctgtgattgg cttcatggta
    54661 gaaccccagt cacgatcagt taaaacagct tggttagtcc caaatgaaac attttcatct
    54721 tgattacgtc cgccaagctg agcaataaca tgaccatttg tcacatcaac aatcgttgaa
    54781 gcaacttgga aatcttggtc tggatagtaa acataatcac cagaatgata aatattataa
    54841 agagtctgct gcgcatcggg gataatattg gtataaactt ttaaaccagc ggtaaaaata
    54901 tctttattcg tttctttttt gacttcttca ataacttgtt ttagataatt atccatatat
    54961 tttggataag ttgagcgctg ttggagtgat tgtagacctt cagcgacggg agttgcgatg
    55021 gcagtttcat attctgcttt cgtcagatgt ttttccatgt acatctgttg caacacgacg
    55081 ttacggcgat tttgagcagc ttcaggatga aggtaaggat catattgact aggagcttga
    55141 gggattccag ccaatagggc tagttgggca taagataaat ccttaagatc cttgccataa
    55201 taagacttag cggctgtcag cataccatag ttgccattac ccatatatac tttgttgatg
    55261 taaaaagtca ggatttcttg tttagtatat tttcgctcca tttgaagagc aagccaaact
    55321 tcttgagcct tacgttttaa ggtttgatca gattcattag tagaaaaata ggctagttta
    55381 atgagttgct gatcaagcgt tgacccccct tgagtggtct gactcgttag attatgaaag
    55441 gcagcaccaa aaatacgata aagatctact ccacgatggt taaagaaacg tttatcttca
    55501 attgaggtaa tagcattaac tagattaata gggatactat cagctgttac attttcacgc
    55561 ttttcagaac ccaaatcagc aatcagattg ttattaccgt cataaaccaa gctagagttt
    55621 gttgatttta actgggcttc tgacagtttc ggagcactgc taatgtagaa ggtaaacaaa
    55681 agaccaccaa taataataac aaggataata aggctaagaa ttgcacttaa tacatacttt
    55741 agccacttaa ggatttttgg atttttaatt gtaatcaccg cctaaaaaat tttgttcaat
    55801 tatgtttaaa taagggactt gtggaaaggc tccaaatgct accttaaagc cattttttct
    55861 gatataatca atgggcatcg atttactgcc attatctatc tgataaaatg aaatcaaggc
    55921 ttgagcaggt aggtagtaag tctccttcag tgtggaaaag tgaagaagga caaagcagat
    55981 tcctttttga tgtaaaacgc aagccatatg ttcaatctga tgtaaatgga aatttttcat
    56041 gggcatagct gttttttgcc tggtttcttt tgcttcaaaa tcaacataat gtcctttata
    56101 aacgccgcaa tagtcagtcg ttgaagcttg tctaaagtac gcttcaacga tttttgcccg
    56161 actgcgttta gggtaatcca ctttgacaat ttggacagga gtaggttttt tgtgaatgac
    56221 agctatttga cgagataaat aataatcatt tgtcgcgttg atagcagctt caaaggacat
    56281 acctctattg gcgaagtcca ccttattttg ctttgtttgc ttttgaacgg aacttacttt
    56341 ttgtcgaata agattatgag gataattaac cattgttctc ctaatattat ctagttaatg
    56401 ctaaaaattt aagcactttt attataacat attttttgaa aggataacac ctaatgactg
    56461 cgattctaat tacaggttac cgaagctttg aaatagggat ttttgaccat aaagacccac
    56521 gagtttcaat tattaaacag gctattcgta aagatttaat aggttatttg gaaaatggtg
    56581 tggattggtt tatctttaca gggaacttgg gttttgaaca atgggctttg gaagtagcta
    56641 atgagctaaa agaggagtat cccttgcaga tagctactat attcttgttt gaaacgcatg
    56701 gagataggtg gaatgaaaaa aatcaagaag ttttaagtca gtttagggct gttgactttg
    56761 taaaatatta ctttccaaat tacgaacaac cgacgcaatt tagtcaatat tatcagtttt
    56821 tattagaaaa gacagaaggg gcttatgttt tttatgacac agaaaacgag accaacttaa
    56881 aatattttct caagaaagca aaagatatgc cgcattatca gttgctattg ctaacctttg
    56941 atcggctaaa tgatatgtca caaagctagc attgtatttt taggggtatt ggtatttaaa
    57001 tacttgcttt ttgaactatt tttctgtatc attaataata atactgaaat ataattttgg
    57061 taaaaagatt gaccgttcgg agagtaaaga atgacaagta ttatttatag tcctaaagat
    57121 atttttgagc aagaatttaa gactagtatg cgagggtttg ataagaaaga agttgatgaa
    57181 tttcttgata atgtaattaa agattatgaa aatttcaatg ctcaaattga agctttaaaa
    57241 gctgagaacg aagccttaaa aaaggccaag tttcaagcta gaaatacagt ttcagcgact
    57301 gttcaacaac cagtaccgca accgactcgt gttgctcaat cagctactaa ttttgacatc
    57361 ttgaaacgta ttagtaaatt ggaaaaagag gtttttggta aacaaattat tgagtaatga
    57421 aaactgtgca atttttggat aatcgcgtag tattttaata ctatgaggaa agtccatgct
    57481 agcactggct gtgatgccag tagtgattgt gctaggcgaa cacataagcc tagggatgtg
    57541 catacacatt acggcgaagg aaatggctaa gtcgtttgat atgccaaagt acttctgaaa
    57601 gtgccacagt gacgtagttt ttatggaaac gtaaaaaatg gaacgcggta aacccctcaa
    57661 gctagcaacc caaactttgg taggggcatg ggatagttgg aaacgaacaa gctatcctga
    57721 ctgtcaacag acggtagaca gatgattatc gaaggaaata attcctagtt atttccggaa
    57781 caaaacatgg cttatagaaa attgcataat aggttaagct agcacttgct agcttttatt
    57841 tgatttaaaa gaaagagata tatgaaagaa acatttagat tagtcgcaac tgcagccgca
    57901 ggtttagaag ctgtcgttgg taaagaagtc agagcactag gttttgattg tcaggttgaa
    57961 aatgggaaag tttattttga aggtgatgta gaagcgattg ttaaaacaaa tctttggtta
    58021 cgtgcagcag atcgtatcaa aattatcgta ggacaatttc ctgctagaac ctttgaagaa
    58081 ttattccaag gtgtctttgc cttggattgg ggaaattatt taccgctagg agctaagttt
    58141 ccaatctcaa aagccaagtg tgtcaagtca aagctacaca atgagccaag tgttcaggcg
    58201 attaccaaaa aagctgttgt taagaaactg caaaaacatt tccacaggcc agaaggagtt
    58261 cctcttcaag aagttgggtc aacgtttaac attgaggtat ctattttaaa agaccaagcc
    58321 actattatga ttgacacgac aggatctagc ctatttaaac gagggtaccg cgttcaaaag
    58381 ggtggggctc ctattaaaga aaatatggca gctgctatct tggccttatc taactggttt
    58441 ccagataaac ctctggttga tcccacctgt ggatcgggaa cgttttgcat cgaggcagcc
    58501 ctgattggga tgaatattgc tccaggattt aaccgttcgt ttgcttttga agaatggagc
    58561 tgggtagata aagacatggt gcaacaagtt cgtgatgatg ccgaacaaga agctaattat
    58621 gaaatcgaat tagatatttc tggctttgat atcgatggcc gcatgattga gattgctaaa
    58681 agtaatgctg aagaagctgg tttatctgat gtgattacct ttaagcaaat gcgtctgcaa
    58741 gactttcgaa cagacaaggt taatggtgta gtgatatcaa atcctcctta tggagaacgg
    58801 ttgcttgatg acaaagcggt tgacatcttg tataatgaaa tgggacaaac ctttgcacca
    58861 ttgaaaacat ggagcaaatt tatccttaca agtgatgaac tttttgaact aaaatacggg
    58921 caaaaagctg ataaaaaacg taaactttat aacggcaccc taaaagttga tttgtaccaa
    58981 ttttatgggg aaagggtaaa gcgacatctg atatagagag gtagtaagcg tgtcagaaga
    59041 aagcaaagaa gtcgaagtaa caaaagaatc gcaaacattg gggcttaatg aagccaagtc
    59101 aatgacaatt ggtgaagctg tacgcaaaca gtcagagata aaagcaggtg ttactaagga
    59161 tgatagtatt ttagataagt acattaagca acaccgtgac gaagtttctt ctcaaaagtt
    59221 tgatgctaag tatacagagc ttgatacagc tagtttagat aactttatca aaaaacaaag
    59281 agaagcattg agtaaagccg gtcttgttga tgacgaacct gtgtctgcgg agtctgctga
    59341 gcaagatagc actttggttg aagaagtagc tgaagactta gctccaatgg agactactgc
    59401 ggtggtaact gggattccgg tggaagcgac agtccctgtg ttagatctag atccatcaga
    59461 gaggggcatt ccagaaccac agatgaccaa ggaagagcca aaaagagatc agtttctttc
    59521 tgaagatagc caccatccag ccaaacaaaa cacgaaaaaa ggttggttaa tagctttatt
    59581 tctactatta ttggccattc ttgcagttgt atttggttgg aaccatttct tgagacaaga
    59641 cagtggtaaa acaactcaaa cagcaagtaa acagacaaag acctctttgc agaccgacag
    59701 tgctaaaaaa gcaacgcgct tgaaggctgc tgctaaagct ttcgaaaaac aatatggtac
    59761 tttttataca gatgccacaa agagcaaatt aaaaaatagt gcctttgcta ctttgccaga
    59821 tttagaagct gccttgaaag ctttggaagg ctctgcttac tatgataagg cgaaagcaaa
    59881 agtcgattcc cttaagaaag ctattgctgc tattacagcg gttaacggta aatttgtttc
    59941 cgatgttgtt gtagatggtg aaaaggtttc ggctgaagtc aaggctgatg ctaattttga
    60001 tgatttatca agtgcaacct tgacaacagg aaatgcaaat cttgatgctg tcttgcaggc
    60061 aagcatcaca gaaggtcgtc agcaactagc cagcaaggct gaggccgcta aagcagctaa
    60121 tgagcaagca gcccaggctc aagcagctca aggacaaagt acatcagtag caccatctgg
    60181 ctatggcttg acaagttatg accctgcaag cttacaacgg catttatcac gtgtacctta
    60241 caatcaagat gttattgctg acagagctaa cccatcttgg gcatttaatc caggagttct
    60301 tgaaaaaatt gtagcaacct cacaagctag aggttatatt tcaggctacc aatatatttt
    60361 agagccggtt aatattatta atggcaatgg ttactataat atgtttaagc ctgatggcac
    60421 ttatctgttc tctattaatt gtaagacggg ttattttgta ggaaatggca aaggttatgc
    60481 tgatgcttta gattactgag aaaatcaaaa aaaactgaga taaggcaact tgtctcagtt
    60541 ttttgattaa taaagtatgt tgtcttaaaa tctgcagata gacttgtttt ttgtaacaag
    60601 tcaaataggt cagtaggatt gttatgtttg acaacacact agtatcagat gacatggcga
    60661 ctaaaaggat cgtctgaaat cccttgatca ataatcaatt gagcccattc tttggcggca
    60721 aagaggctat gatccttata gtttccacag gattcaagag ttgttccagg aacatcttcc
    60781 caagtaatcc cagttgcaat ttcttctagg ctggatttaa tcaccttggc aatatcagta
    60841 gaactgtgtt ttccccacat gataaggtga aaacctgttc gacagccaaa aggagagcaa
    60901 tcaatcatcc catcaatgcg ttggcggatg agcttggcaa gtaagtgttc aatggtatgc
    60961 aaaccggctg tttcaataga attttggtta ggctgcacta ggcgaacatc aaaatttgta
    61021 atacgatcgc ccttgggtcc aaattcttca gaaataagac gaacataagg ggcctttaca
    61081 atagtatgat ctagctcaaa actttcgacg ataacttctt ttgtcatttc aatctccttt
    61141 gttttacact ttatcataac acaaatagtg agaaagtggc tacttgtgga ggagaaaagg
    61201 ctaagaaaac agatcaaagg ctatcataat gacctgtgcg ataaaagtac ttacagaaac
    61261 agtaataaag gtttctttaa aagcattttt gtggttaagt cctgtcaatg ctaagaaggt
    61321 gagaaagaca ccactttggg gcacaatggt aaagatatta gaagcaatag tggcaacacg
    61381 gtgaatcatt tcgggattaa ggccttgatc taaataatac tgggcaaagt taggcatgac
    61441 aattcctaga gcaccggaag aagagccggt aatagctgac atagaggaag tcaacacggc
    61501 taaactaata agaggattac cgggaatatt caaaatgaga tctgagaaga atgtgaaacc
    61561 tggggcaatc atcacaacgg ctccgaaggc aactgcagat gctgtcgcaa aaataggtgc
    61621 aatagagctg ctagctccta aattaaagac agcaatctta ttaggaataa actgtctaaa
    61681 gagccagctt gcggtgagaa tggcagctaa aagtgctatg aaaataatgt tcttcttaaa
    61741 aaaatcgttt ccgaaaaggc taccggttag ggcgataata atgagcaaca ataaaggtaa
    61801 gatactggca aggaaatgtg ggagattttt tgttttcact tgtatctcat tgtcaaaagc
    61861 ataagtggca taagtttcac cccttgccat actttttgct agacaatatt tcatataaaa
    61921 gagcccaaaa gtcacgcagc caattgagcc aacgatactc ggaatggcgg cagcagttag
    61981 tgaggtgtcc aagtattgaa taggaataac attttgaatg gcaggtgttc cgggtaaaat
    62041 cgtcatggta aaagttgcaa taccgagcca aagaggaact tgaattaagt tccaagccaa
    62101 gtccattttt ttgaatagac ttcgtgcaag cggcaagaca gcaaacatga caacaaagag
    62161 gctgattccc ccgtaagtta atatagcact gattagaaaa atagcaacga gtactttata
    62221 aggactatca tgaccaactt tttttaaaat ataatctgca atagaagtgg tttcaccgct
    62281 tgtttccatt aatttggcta agatagaacc taataaaaag atagcaaaat aattgagaat
    62341 ataagtggag agtgccccca taaattggtt gggttctttt ccaagtaagg ttgtggttgg
    62401 atccatttga ttaaacaaaa tgaccaagct ggtagcaaga ggcgcagcga tgataatgtt
    62461 aacttctttg acagacaggt aaataataac aataacacct atcaagaccc ccagagaacc
    62521 aataatttcc atagttttat gacctcctta ttagaaaata aagagacaga tcacaagata
    62581 cagcctcttt atctctccaa atttattgag cagtgtagcc accgtctaag atacaggctt
    62641 gacctgtaac accttttgcc ttatcactgg caaggaaaga cacatagtct gcaatttctt
    62701 gaacgtcaat gaggcgtttt tgaggaacaa gtgggtatag cacttcttca agaacatttt
    62761 caaggggaat acctcttgtt ttggaaaggt cttcaaactg gccacgaacc agtggtgtgt
    62821 caacatatcc aggacaaatg gcattgaccg taatgcctga atcagcagct tctaaggcag
    62881 ttactttggt cagaccgatc aagccgtgct tagctgaatt gtaggcggat ttgccagcaa
    62941 aaccaatgac accattgata gaagccatat taataatacg accgtgtttt tgagctttca
    63001 ttgtaggaaa agcacgttta atggcaataa atggtgctgt caacatgatt ttaatcatga
    63061 attcaaattt ttcagttgga aaatcttcaa tcatggcaac atgttgaagg cctgcgttgt
    63121 taaccaaaat atcaatacga ccatattttt caacaaccgt atcaatagca gcattgatgg
    63181 cttcttcttt ggtgacatcg cacacaacac taaaagcgtc atagccttct ttagtatagt
    63241 cagcaacaac ctgatccagt ttttcttgat taatgtcaga aaaaacaacc gttttacctt
    63301 cttttaagaa ggtttccccg atttgttttc caatcccgct tgcagcacca gtgataaatg
    63361 caacttcttt agtatgtgtc atgatactat cctcgtcttt cttttggtta gtctttagtt
    63421 tgttttatca attagggtaa cttcggtaac gttttgcacg tcttcaaagg tgtaatctgg
    63481 gttaatttca agggcgcata gaccttcatc ttgatattca aagactccca tttcagtaat
    63541 gattaggtta acaacatttt gagcagtgag aggaagggtg catttgtcta aaatttttgc
    63601 tttgccttta ttggtatgct ccattgcaac aatgactttt ttagcaccta ccaataagtc
    63661 catggcccct cccatcccag gaaccatttt tccaggaatc aggtaattag caatgctagc
    63721 ttctttatca acttgtaagg cacctaagac ggttgcggcc acatggccgc ctcgaataat
    63781 accaaaggaa gttgagctat cgaagaaagc ccctccagga gcaatactaa caggttgccc
    63841 accagcatta acaatactag gatcgtagtg atcatctgtt aatccagtga gaccgacaaa
    63901 accattttcg gactgcaggg taatggttac accttcagga atatagttgg ctacttttgt
    63961 tggcaaacct atgcctaagt tgaccagtgt attgtgttct aattcttgtg caacgcgctt
    64021 agcaatacgt gtttgaatct cttcttttga aagaacagta gctgatgttc ccatgttagg
    64081 ctcctttcac taagtagtta acaaaaatgc caggagtatg gacaaaatta ggatccattt
    64141 gccccacgtc aacaatttct cttgcttcaa caatggtggt tttcgcatta gcagccatca
    64201 catgattaaa gttattttca gaacctgcgt attgcaaatt gccattttta tctgccttgt
    64261 tagcaaaaat aagtgcgata tcagctttca aaggtttttc aagaaggtag tctttgccgt
    64321 caacagtaat gacttctttt cctttggcaa cttcggttcc tattccagta ggtgttaaga
    64381 aaccgcctag cccaaaaccg ccactgcgga tgcgctcagc taatgttccc tgaggtacaa
    64441 gatcaattac agtctcgccc tcagtcattt gtcggcccgc ttcacggttg agaccgatat
    64501 gtgatgcaat aatagttgaa aactgtttat tggcaaccat cttaccaacg cctttgtcag
    64561 gaaaaccggc atcgttacag attaaagtga ggtctttgac acctttttcc acaagtgtat
    64621 caattaattt ttctggtgtg ccatttgtca tgaagccacc aaccataatg gtatcaccat
    64681 ctttaacgtg agctaccgct tcttttattg tgatttcttt acacattttt tctaccaact
    64741 ttcacgagac taagtattgt ttttcacgat aacagcagtt ccttgtcctc caccaatgca
    64801 aagtgttgct agtcctctgg ttacttggcg ttttttcatt tcgtggatta atgtgactag
    64861 aatgcgagcg ccactggcac caataggatg acctaaagcg atggctcctc cattaacatt
    64921 cacgatttct gggtttaact ttaaatcttt taaaaccgcc aaggactgtg ctgcaaatgc
    64981 ttcattggac tccactaagt ccaaatcaga aattgttaat ccagctttct taagggcttt
    65041 ttgagtggca ggaattggtc cagttcccat cagttcaggt gcaacacctg cactagcata
    65101 gctttcaata gtgactaaag gtgttaaccc taattcaaga gctttttctt cagtcattaa
    65161 catcaataag gctgcaccat cgtttattcc tgaggcattc ccagcagtaa cggttccttc
    65221 tttagggagg aaagcaggac gtaattgctg taatttttca aggcttgtat caacttttgg
    65281 gtattcatct gttgtaacaa ttaaagggtc tttgcgtcgt tgaggcacag agacagggac
    65341 aatttcgtca gcaaaacgat gattttctaa tgctttagca gctttttctt ggctttctaa
    65401 agcaaattga tcttgctctt ctcttgagat gccatatttc tgagcaacgt tttcagcagt
    65461 aatacccatg tggtaattgt taaaagcatc tgttaagcca tcatgaacaa gagtatcaat
    65521 caattgacta ttaccaagac gttgaccaaa gcggtgctga gttgagacat aagctgcttg
    65581 tgacatgttt tcgacaccac cagcaataac gatatcagcg tcaccaagca tgatgctttg
    65641 cgcagctaat tggattgctt taagtccaga accgcaaacc atatctaatg tgaaagctgt
    65701 tttatcttca ggaattcctg agtggactgc aacttgtctt gccacatttt gtcctaggcc
    65761 tgcatgaagg acgtttccta ggatgacttc atcaaccatt tcaggcttta cttgcgtttt
    65821 atcaaggatt tttttcagta cagttgttcc aagttcaaca gcagagacac ttttaaagga
    65881 gcctccaaaa gctccgatag gagttcgtaa agcttcaacg ataactatgt ttttcataaa
    65941 ataaacatcc tttcagtttt agaaagcgct ttccatgtct ctaagataac aagaaaacgc
    66001 taaaaataaa aatatagaat ttttataagc atataatttt ttttgataga gttggcaata
    66061 gcatttaaca tctacttgtg tcataatata ggtaattata ccggaaaaag taattgataa
    66121 gacaaggaga atcctatttg gatattaaac aaattcgtta ttttatcgca atcgttgaaa
    66181 atcattttaa cttgagccag gcggcggaac tgttgtatgt ttctcaaccg acgctgagta
    66241 tgatgattaa cgattttgaa aaaagagaaa atgttaagct cttcaaacga aaaagaggtc
    66301 gtattattgg gttaacctat cttggtgata attattataa ggacgctcaa aaggttctca
    66361 gtctctatga tgacatgttt ttgaaattgc atgatcacag taaagggtta aagggaagta
    66421 tcagtattgg tattcctcct cttattttat cagttgtttt ctcagaagta atgccaaaac
    66481 tgattttaga aaatccgggg attcaattca atgttaagga gattggtgct taccaactaa
    66541 aaaatgaact actcgtggga aatgttgacg tcgcagtctt gttatcccca acaggcatcg
    66601 ctgataattt ggttgagact tatgaaatcc agagatcaga attatctgta tgcctgtctc
    66661 cacgccatcg tttagcttcc aaaaaagtta ttcagtggga agatttaaca gatgaacaac
    66721 ttgccctatt tgatcctagt ttcatggttc accatttagt gcttgaagct tgcgagcgtc
    66781 accaagtcag acctaatatt attttgacgt cctcttcctg ggattttatg cttaattcta
    66841 ccaagattaa tcacaacgtc ttaactattt gtcccaaacc tattacagaa ttataccaat
    66901 tcaaggacat taagtgtatt ctaatggaac gtcccatttc ttggcgtgtt gtcttaacac
    66961 gcctccggaa aaaaagttat tctgaaatag aagcctacat catggacgac ttgctacagt
    67021 ctttttttaa gtagggttct caactacttg gcccaaatgt taattttttt aaaaatgaat
    67081 tggaattgtg taaacgctca atttatgata aactaagaga tggctttcta ttaatgaaat
    67141 ctagaatata ttgaggtaca aacatggtta atattatttt attaattgtt tctgccctca
    67201 ttggtttaat attaggttat gcacttattt cgattagact caagtctgcg aaggaagctg
    67261 cagagttgac tcttttaaac gctgaacaag aagctgttga tattcgtggc aaagcagaag
    67321 tagatgctga acacatcaaa aaaacagcta aacgtgaaag taaagcaaat cgtaaagaat
    67381 tacttttaga agcaaaagaa gaggcaagaa aatatcgtga agagattgaa caagaattta
    67441 agtctgaaag acaagagctt aaacaactcg agacacgctt agcggagcgc tccttaactc
    67501 ttgaccgtaa agatgaaaac ctatcaagta aagaaaaggt actagatagt aaagaacaaa
    67561 gtctgaccga taaatctaaa cacattgatg agcggcaact tcaagtggaa aaacttgaag
    67621 aggagaaaaa agcagaactg gaaaaagttg ctgcgatgac gattgcagaa gcgcgtgaag
    67681 tgattttaat ggagacggaa aacaaactga cccatgaaat tgcgacgcgc attcgagatg
    67741 ccgaacgtga catcaaggac cgaacagtta aaacagccaa ggacttgtta gcgcaagcca
    67801 tgcaacgcct tgctggtgag tatgtgactg aacaaactat taccagtgtc catctcccag
    67861 acgacaacat gaagggccga attattggac gtgaaggccg taatattcgt actttagaga
    67921 gcttgactgg cattgacgtt attattgacg atactcctga agttgttatc ttatcaggat
    67981 ttgatcctat tcgacgtgaa attgctcgta tgaccttgga atctctgatt gctgatggtc
    68041 gcatccatcc agctcgtatc gaggaattgg ttgagaaaaa tcgtcttgaa atggataatc
    68101 gtattcgtga gtacggtgaa gctgcagcct atgagattgg tgcaccaaac cttcatcctg
    68161 atttgattaa aatcatggga cgcctgcaat tccgtacctc gtttggtcaa aatgtcctac
    68221 gtcactctgt tgaggttggt aagttagctg gtattttagc tggtgaactg ggtgaaaatg
    68281 ttgcccttgc ccgccgcgct ggtttcttgc atgatatggg taaagctatt gaccgtgagg
    68341 ttgaaggtag tcacgttgag attggaatgg aatttgcacg taaatacaaa gaacatccag
    68401 ttgttgtcaa cactattgct agccaccacg gagatgtgga gccagattct gtgatcgctg
    68461 tgctagtagc tgcagcagac gctctcagtt cggctcgccc aggcgctcgt aatgagtcaa
    68521 tggagaatta catcaagcgt cttcgtgatt tagaagaaat cgcgacaagt tttgatgggg
    68581 tacaaaatag ttttgctcta caagctggac gtgagattcg tattatggtt caacctgaaa
    68641 aaatttcaga tgatcaggtt gtcattttgt cgcataaagt aagagaaaaa attgaaaaca
    68701 atctagatta cccaggaaat attaaagtaa ctgttattcg tgagatgaga gcggttgatt
    68761 atgccaagta gaaaaagagg aattatcctc tttttcttta tgacttgcct ttgaaaatgt
    68821 tcttgaaaaa gactagcctt tttgataaac tattaaaaag caactaataa ggagaatata
    68881 atgtctgaac gcggtttgtt aatcgtcttt tctggccctt caggtgtcgg aaaaggaacg
    68941 gtaagacaag aaattttttc aacgccagat cataaatttg aatattcggt ttcaatgacg
    69001 actcgtccgc aaagaccggg cgaggttgat ggggttgatt atttcttccg cacacgtgaa
    69061 gaatttgaag aactcattaa aactggtcaa atgctagagt atgctgaata tgttggtaat
    69121 tactatggca cccctttaac ttatgttaat gagactcttg ataagggaat tgatgtcttt
    69181 ttagaaattg aagttcaagg agctcttcag gttaagtcaa aagtaccaga tggtgttttt
    69241 gtttttttaa caccaccaga tttagatgaa ttagaagacc gcctggttgg tcgtggtaca
    69301 gatagtcaag aagtcatagc tcaacgtatt gaaagagcaa aagaagaaat tgcccttatg
    69361 cgcgaatacg attatgcagt tgttaatgat gaagttgcct tggcagctga gcgggttaag
    69421 cgtatcattg agacagaaca ctttcgtgtg gagcgcgtga ttggtcgtta tgataaaatg
    69481 attaaaataa caaaaaatcc ctttaaagct aaatagaaaa gagtaagcat tatgatgtta
    69541 aaaccttcca ttgatacctt attagataaa gtaccatcta agtattcact tgtgatttta
    69601 caagccaaac gtgcccatga actagaagct ggtgccactc caactcaaga atttaaatca
    69661 gtgaagtcaa cacttcaggc acttgaagaa attgaatcag gaaacgttgt gattcaccca
    69721 gatccttcag ctaaacgtga agctgtccgt gctaagattg aggcagaacg cttggccaaa
    69781 gaggaagagg aacgtaaaat caaagaacaa attgccaaag aaaaagaaga agaaggagaa
    69841 aaaatctaag gtttcagaaa tcttagattt tttcatattt agtacgatgt ccatgacaga
    69901 aaaggagaag caaatggaaa aagtagctca tgtgattgta gatattccat tgatgcaaac
    69961 ggacaagcca ttttcttatg gtattccaaa agagttagtt tctctcgtgc agcttggttc
    70021 tcgtgtgcat gttccttttg gaaaagggaa tcgtttgttg caaggctttg ttattggttt
    70081 tggccaggaa gattcaagct ctcttaaact gattcagacc gttttagatc ctgagccagt
    70141 cctaaaccaa gaacaattga cccttgctga tcagcttcga aaaacagttt tttcttataa
    70201 aatcacgctc ttaaaagcaa tgattcctaa tcttcttaac tctaactatg ataaagtgct
    70261 aaggccagag tcaggtctaa aaaagcctga tagggacttt ctttttgaag gaaaaccatc
    70321 tgtcttatat tctaccttag accgcgagaa ggaaaaaata gctcttaagg gcatacaagc
    70381 tgggcatatt actgttagtt atttagccaa agacaagaaa aacctaaaga cagaaaaata
    70441 ttatcacgtc gacttagacg ccttagcggt acatcccatc tctagtcgtg ccaaaaagcg
    70501 tcaattatta aaagactatc tgctaaccca tacgaaggaa gctaaacttg caacgcttta
    70561 tcaagccttt tctagagatg tggtggctta ctttgtgaca aatcacttaa ttcgtattga
    70621 cgaacgtcaa attgatcgca gtgagcccta ttttgaccag atcaaaccat cttctttttt
    70681 aaccttaaat gaacagcagg catcagctgt tactgaaatt gttgagcaaa ttgggaaacc
    70741 gagtaaacct tttttgattg aaggtattac aggttcgggt aagacagaag tttatcttca
    70801 tatcatcgaa gcggttttaa aacaggataa gacagccatt gttttagtac cagaaatttc
    70861 gttgactcct cagatgacct ctcgttttat ttcccgtttc ggaaagcaag tggctattat
    70921 gcattcaggc ttatcagatg gtgaaaagtt tgatgagtgg cgcaagataa aaacaggaca
    70981 ggctaaggtt gtggttggtg cccgctctgc tattttctct ccactagagc ggattggtgc
    71041 aattattatt gatgaggagc atgaatcaac ctataaacaa gaaagcaacc ctagatacca
    71101 tgccagagag gttgccctgc tcagagctaa acaccatcaa gcagttgtgg ttatgggctc
    71161 tgctacgcca agtattgaaa gccgagcaag ggctagtaag ggtgtctatc attttattca
    71221 attaacgaaa agagcaaacc ctcttgctaa gattccagaa gtaacgattg ttgattttcg
    71281 tgattatatt ggacagcaag cagttagtaa ttttacccca tatttgattg ataagattaa
    71341 agaacgtttg gttaaaaaag aacaagttgt cttaatgtta aatagacgtg gttattctag
    71401 ttttgtgatg tgtcgggact gtggttatgt ggacaaatgt cccaactgtg atatctcttt
    71461 aacccttcac atggatacta aaactatgaa ttgccattac tgtgggtttc aaaaacctat
    71521 cccaattact tgtcctgagt gtcatagcaa cagtatccgc tattatggca ctggaactca
    71581 aaaagctttt gatgaattgc aaggggttat cccagaagct aggatcttga gaatggatgt
    71641 tgacaccaca cgaaaaaaaa gaagtcacaa aaccatacta gatagttttg gtcgccagga
    71701 agcagatatt ttgttaggga cgcaaatgat tgccaaagga ctggattttc caaatgtgac
    71761 tttagttggg gtactaaatg cagatacctc cttaaatttg ccagattttc gagcgtcaga
    71821 aaaaacgttt caattgttaa cacaggttgc tggtcgggca ggccgtgccc ataagccagg
    71881 ggaagtcttg attcaaactt ataatcccga tcactatgct attcaattag ctaaacaaca
    71941 agattttgaa gccttttacc gttatgaaat ggcgattcgc cgtcagatgg cttatccccc
    72001 ttactacttt acagtgggta ttacgctttc gcatcgttta gaagcatcgg ttgtaaaaaa
    72061 agcttatcaa gttacagaac tgctaaagag tcacttatcg gataatatta aaatattagg
    72121 accgactcct aagccaattg ctagaacaca caatttatac cactatcaaa ttttgttaaa
    72181 ataccgtttt gaagataacc tagaagagac gctcaatcgt attcttgatt ggagtcaaga
    72241 agcagataat cgtcatttaa aactaattat tgattgtgaa ccgcaacaat ttttataagg
    72301 taaatcacta aatctaggat gatcagttat gatataataa aaatacaaag aggaaagtca
    72361 tgataaaatt attatttatg ggaactcccc aattttcagc aactgtattg aagggcttgc
    72421 tagataatcc tgcctatgag attttaggag tggttactca gcctgatcga gctgttggac
    72481 gcaaaaaaga tattaaggta acacctgtaa agcaattagc tcttgagcat ggaatatcaa
    72541 tctatcagcc ggaaaaatta tcaggctctc aagaactcat tgagattatg gaacttggtg
    72601 cagatggtat tattacagca gcatttggcc aatttttacc aacaatatta cttgattcag
    72661 tttcatttgc tattaatgta catgcatcat tgttacctaa atatcgaggt ggagcaccta
    72721 tccattatgc tattatgaat ggagataaag aggctggcgt tactattatg gaaatgataa
    72781 aagaaatgga tgcaggtgac atggtcgcta aagcaagcac tccaattctt gaaaccgata
    72841 atgtaggtac tctttttgag aaattagcca tcattggtcg agacttactt ttggatagtt
    72901 tgccagctta tctttctgga gaattaaaac ctattcctca agatcacagc caagctactt
    72961 tttcccctaa tatctctcca gaacaagaaa aattagattg gacaatgtct gatcaagaag
    73021 tgttcaatca tattcgaggt atgaaccctt ggccggtagc gcatacgttt ttagaggggc
    73081 aacgtttaaa aatttacgaa gctcaattag ctgaaggaga aggattacct ggacaagtta
    73141 ttgtcaaaac caaaaaatct ttggtaatag ctgctggtca aggagccctg tcattgctgg
    73201 ttgtgcaacc tgcggggaaa cctaaaatgg ctattgcaga tttcttaaat ggccttggta
    73261 gaaaacttga agtgggtgat ttcattggca gataattgga aaataagtat tcgaggaaaa
    73321 gcacttctaa ccttagaaca tatttttgat cagggggctt acacaaacat agcgcttaat
    73381 cagcagctaa gcaataaagc tttatctgct aaggacagag ctctattaac tgagattgta
    73441 tatggtaccg tttcacgtaa aattagcttg gaatggtatt tagcccatta tgtcaaagac
    73501 cgagataaat tggacaaatg ggtttattat ctccttatgc tcagtcttta tcaactgatt
    73561 tatttagata agttaccagc gcatgccatt gttaacgatg cggttggtat tgccaaaaat
    73621 cgtggcaata aaaaaggtgc agagaagttt gtcaatgcta ttttacgtca attcaccagc
    73681 catcctctcc cagatatgga aacaattaag cgtcgaaata agtattattc ggtcaagtat
    73741 tcactgcctg tttggttggt taaaaagtta gaagaccaat ttggttctga cagatcagtg
    73801 gccatcatgg aaagtttatt tgtgcgtagt aaggctagta ttcgcgtgac agaccctttg
    73861 aagctagagg aagctgctga ggctttagat gcagaacgtt cccttttatc agcaactggt
    73921 ttaaccaaag cgtcaggtca ttttgcagcc agtgattatt tcacaaatgg ggacatcact
    73981 attcaagatg aaagcagtca gcttgtagct cctactttaa acattgacgg tgacgacata
    74041 attcttgatg catgtagcgc tccagggggc aagacctctc atatagcatc ttacttaaaa
    74101 acaggtaaag ttattgcctt agatctctat gatcataaac tagagttggt gaaggaaaat
    74161 gctaaccgct taggagttgc tgataacatt gagacaagaa aactagatgc cagagaggtt
    74221 catcgtcatt ttgaaaagga tagctttgat aagattttgg tggatgctcc ttgctcagga
    74281 attggtttga ttcgtcgaaa acccgatatc aaatataata aagaaagtca agatttcaat
    74341 gctctacagg ctatccagct agagatattg tctagtgttt gtcaaacctt gcgtaaaggt
    74401 ggtataataa catacagtac ttgcactatt tttgatgagg agaatcgtca agtcatcgaa
    74461 gcatttttac aaagtcatcc taattttgaa caagtaaaac tgaaccatac acaagcagat
    74521 attgtcaagg atggttatct tattatcaca ccagaacaat accagactga tggcttcttt
    74581 attggacaag tcagacgagt cttgtagtat tttaggagga aactgactga gtcagaaaaa
    74641 acgtatgaag atttcattaa aaacagacat tgggcaaaaa cgctcaaaca atcaagattt
    74701 tatcaataag tttgataata aaaaaggcat cactttagtg attttagcag acggtatggg
    74761 tggccacaga gctggaaata ttgccagtga gatgacagtc acagacctgg gaagagaatg
    74821 ggtaaaaaca gacttcacag agttaagtca gattagagat tggttatttg aaactatcca
    74881 gtcagaaaat caacggattt atgatctagg ccaatcagaa gactttaaag gaatgggaac
    74941 gactgttgaa gcagtagctt tagttgaatc tagcgccatt tatgctcata ttggtgattc
    75001 acggattggt cttgtccatg atgggcatta taccttattg accagtgatc attctttagt
    75061 gaatgagttg gttaaggcag gacagatcac agaagaagaa gctgctagcc atccacaaag
    75121 aaatattatc actcaatcta ttggtcaagc ttccccagta gagcctgatt taggagtgag
    75181 ggttttggag ccaggggatt atcttgtcat taacagtgat ggtctaacca atatgatttc
    75241 aaatgatgaa attgtgacta tcttgggcag taaggttagt cttgatgaaa aaaaccaaga
    75301 aatgattgac ttagctaacc taagaggagg cctagataat attacaatcg ctttggttca
    75361 caacgaaagt gaggacgtcg aatgattcag attggcaaat tatttgctgg tcgttatcgc
    75421 attctgaaat ctattggccg cggtggtatg gcggatgttt atttagcaaa tgatttgatc
    75481 ttggataatg aagacgttgc aatcaaggtc ttgcgtacca attatcaaac agatcaggta
    75541 gcagttgcgc gtttccaacg agaagcgcgg gccatggctg aattgaacca tcccaatatt
    75601 gttgccatcc gggatatagg tgaagaagac ggacagcaat ttttagtaat ggaatatgtg
    75661 gatggtgctg acctaaagag atacattcaa aatcatgctc cattatctaa taatgaagtg
    75721 gttagaatta tggaagaagt cctttctgct atgactttag cccaccaaaa aggaattgta
    75781 cacagagatt taaaacctca aaatatccta ctaactaagg agggtgttgt caaagtaact
    75841 gatttcggca tcgcagtagc ctttgcagaa acaagcttga cacaaactaa ttcgatgtta
    75901 ggcagtgttc attacttgtc tccagaacag gctcgcggct ccaaagcgac gattcaaagt
    75961 gatatttatg cgatggggat tatgctcttt gagatgttga caggccatat cccttatgat
    76021 ggcgatagtg ctgttacgat tgccttgcaa cattttcaaa agcctcttcc atctattatc
    76081 gaggagaacc acaatgtgcc acaagctttg gagaatgttg ttattcgagc aacagccaag
    76141 aaattaagtg atcgttacgg gtcaaccttt gaaatgagtc gtgacttaat gacggcgctt
    76201 agttataatc gtagtcggga gcgtaagatt gtctttgagg atgttgaaag taccaaaccc
    76261 ctccccaaag tggcctcagg tcccaccgct tctgtaaaat tgtctccccc taccccaaca
    76321 gtgttaacac aggaaagtcg attggatcaa actaatcaaa cagatgcttt acagcccccc
    76381 accaaaaaga aaaaaagtgg tcgtttttta ggtactttat tcaaaattct tttttctttc
    76441 tttattgtag gtgtagcact ctttacttat cttatactaa ctaaaccaac ttctgtgaaa
    76501 gttcctaatg tagcaggcac tagtcttaaa gttgccaaac aagaactgta tgatgttggg
    76561 ctaaaagtgg gtaaaatcag gcaaattgag agtgatacgg ttgctgaggg aaatgtagtt
    76621 agaacagatc ctaaagcagg aacagctaag aggcaaggct caagcattac gctttatgtg
    76681 tcaattggaa acaaaggttt tgacatggaa aactacaaag gactagatta tcaagaagct
    76741 atgaatagtt tgatagaaac ttatggtgtt ccaaaatcaa aaatcaaaat tgagcgcatt
    76801 gtaactaatg aatatcctga aaatacagtc atcagtcaat cgccaagtgc gggtgataaa
    76861 tttaatccaa acggaaagtc taaaattacg ctcagtgttg ctgttagtga tacgatcact
    76921 atgcctatgg taacagaata tagttatgca gatgcagtca ataccttaac agctttaggt
    76981 atagatgcat ctagaataaa agcttatgtg ccaagctcta gctcagcaac gggctttgtg
    77041 ccaattcatt ctcctagttc taaagctatt gtcagtggtc aatctcctta ctatggaacg
    77101 tctttgagtc tgtctgataa aggagagatt agtctttacc tttatccaga agaaacacac
    77161 tcttctagta gctcatcgag ttcaacgtca agttcaaaca gttcttcaac aaatgatagt
    77221 actgcaccag gtagcaacac tgaattaagc ccatcagaaa ctactgctca aacaccttaa
    77281 caaagaatag ttagtctcat gttttactag catgccacta actattcttt ttaactactc
    77341 gtcatataaa gatgcaattt tccaggaatt ctgataaaat acaaagaaat aaggggaaac
    77401 taatgaaaaa atttcaattc tttttactta tcgagtgtat actgctagct atgggtatca
    77461 tgacaatctt ggataatgat ttatcaagtt ttatcctaat tctagttctt atcttattag
    77521 cattacgctt ttataaccaa gatagccgca ataatttttt actaactgtc agtttattgt
    77581 ttcttttttt aattttcatg cttaatccct acattatcat ggctgttctt ttggggatag
    77641 tttacatttt cattaaccat ttttcacaag tcaaaaagaa gaatcgcttt gccttgattc
    77701 gtttcaagga agaaaagatt gaagtgaaca ataccaagca tcaatggatt ggtactgcta
    77761 attatgaaag tgattattat tgttttgatg acattaacat cattcgaatc tcaggaaatg
    77821 acacggttga cttaaccaat gttattgtga caggaatgga taatatcatt gtgatacgta
    77881 aaatttttgg aaatacgact atattggtac ctattgatgt cactgttact ttagatgtta
    77941 gttccatata cggaagtgtt gattttttta gatgtcagca atatgatttg cgcaacgaat
    78001 ctattaagtt aaaagaaacg gataaccaat cccttaaaaa agtcaaaatt atcgttacaa
    78061 ctattgccgg aaatgttgag gtggataggc gatgaaaaaa cgttactatg ctcttgtttg
    78121 gctctactca accattacca ttttatctat tgtttttgtg gtcatggata atttaggaat
    78181 cacttttaac taccttcgca atcatttatg gcaggtcgaa cgtctagggt tttcgatttt
    78241 attgttgatt gtttctgtga ctttattatt gttgttattg tggattatta tggatgataa
    78301 cagtaagcgt aacatcaacc aaaatctaaa atatattctc aataatcgac gactctatct
    78361 agatgagaca tcagaaatca atactaattt aagtcgactt tctaaaaaaa tgtctcacct
    78421 gactgctaac atgcaaaaga aagaaagtgc ttatattctt gatagccaag aagttgtaaa
    78481 acaagagcgc aaacgaattg caagagattt acatgacaca gtcagtcaag agctattcgc
    78541 ttcgtcattg attttatcag gaatttcaat gagtttggaa caactggaca aaacacaatt
    78601 acaaacacag ttaacaacgg ttgaagcaat gttgcaaaat gctcaaaatg atctacgcat
    78661 tctccttttg catcttagac ctaccgagct agctaatcgg actttatctg agggccttca
    78721 tatgattctt aaggaattaa cagataaaag tgatattgaa gtcatttata aggaaaccat
    78781 tgctcagctt cctaaaacaa tggaagataa tctttttaga attgcccaag aattcattag
    78841 caacacgtta aaacatgcta aagctagtcg aattgaagtt tatctcaatc aaacctcaac
    78901 agaattacaa ttgaagatga ttgatgatgg tgtaggattt gatatggatc aggtaaggga
    78961 tttgagttat ggtctgaaga atattgaaga ccgtgtcaat gatttagcag gaaacctaca
    79021 tttaattagt caaaaaggca aaggagtttc catggatatt agactgccga tagtgaaggg
    79081 agatgacgat gagtaagata aaagtgatat tggtcgatga tcatgaaatg gtccgcatgg
    79141 gactcaagag ttttttgaat ttacaagctg atattgatgt cgttggtgag gcctctaatg
    79201 gacgtgaagg ggttgatttg gcattggctt tgaagccaga tgttttggtt atggatctag
    79261 tgatgccaga gttaggcggt gttgaggcaa ctttagaagt tctaaaaaaa tggaaagagg
    79321 ctaaggtact tgtgttaact tcctatctag ataatgaaaa gatataccct gtcattgatg
    79381 caggagcgaa gggttatatg ttaaaaacat cgagtgcggc tgaaatttta aatgccattc
    79441 gcaaggtttc aaagggagag ttagctattg aaacagaagt tgacaaaaaa attaaggcgc
    79501 atgatcaaca ccctgacttg catgaggaac taacagcgcg tgagtatgat attttacacc
    79561 ttttagctaa agggtatgat aatcagacca tcgctgatga actctttatt tccttaaaaa
    79621 ccgtcaaaac acatgtgtcc aatattctag ccaagttaga agttgatgac cgaacccaag
    79681 cggttgtcta tgcttttcga catcatttag tcccccaaga cgataattaa tgctataata
    79741 tagggtatag caaattggag gaaagtatgg acgcaaaact aaaatacaaa gctaaaaaaa
    79801 tcaaaatggt gttctttgat attgatgata ccttgagggt caaagatacg ggttatatgc
    79861 ctgagtcgat tcaaagagta tttaaggctt tgaaggcaaa aggcatcttg gttggtattg
    79921 cttcaggtcg cgctcggtac ggtgttccac aagaagttca agatttacat gctgactatt
    79981 gtgtcaaatt aaatggagct tatgtcaaag acgatgctaa aactattatt ttccaagctc
    80041 cgattccagc agatgttgtt gtggcctata aaaaatgggc ggatgatatg ggcatttttt
    80101 atggaatggc gggtcgtcat gaagcggtgt tgtccgctcg aaatgacatg attagcaatg
    80161 ctattgataa tgtctacgct caactagagg tctgtccgga ttataatgag tatcatgatg
    80221 tttaccagat gtggactttc gaggacaagg gagatggctt gcagttgcct gctgaattag
    80281 cagagcacct tcgtttggtc agatggcacg ataactcatc agatgttgtc ttgaaaggta
    80341 cgtcaaaagc gctaggtgtt tcaaaagtag ttgaccattt aggattaaaa cctgaaaaca
    80401 ttttggtttt tggagatgaa ctcaatgacc tagagttatt tgactatgct ggtatcagta
    80461 ttgcaatggg agtatcacat ccattactgc aagaaaaagc agattttatc acaaaaaaag
    80521 ttaaagaaga tggcatttta tatgccttgg aggaattagg attgattgac aaagaattac
    80581 agtttccaca attggatttg ccaaaccata aaggaccaaa agcaaccatt aaaacaaacc
    80641 atggcgatat gaccctggtc ttgttcccag atcatgctcc taaaacagta gccaatttct
    80701 taggtttggc taaagaaggg tactatgatg ggattatctt ccaccgtatt attcctgaat
    80761 ttatgattca agggggcgat ccaacaggaa caggaatggg tggacaatcc atctatggtg
    80821 aaagttttga agatgaattt tcggatgaac tctataatct ccgtggggcc ttgtcgatgg
    80881 ctaatgcggg accaaatact aacggcagtc agttctttat tgttcaaaat agtaagattc
    80941 cttatgccaa aaaagaacta gaacgtggtg gttggccggc tccaattgca gcttcttacg
    81001 ctgcaattgg tggaactcct caccttgatc gtcgtcatac ggtctttggt cagcttgtgg
    81061 acgaaacttc attccaagtt ttggatttga ttgctggcgt tgaaactggc gcacaggata
    81121 aacctaaaga agacgttatc attgaaacaa tcgaggtatt tgactaatga aaattggcga
    81181 caaactgcat ggcaccatca ctgggattaa accatacgga gcctttgttg cccttgaaaa
    81241 tgggacaaca ggtctcattc atatttcaga aataaaaacg ggatttattg acgatattga
    81301 tcaattattg gctataggca accaggtttt agtacaagta attgatattg atgaatatag
    81361 taaaaagcca agtttatcta tgcggacctt agcagaagaa aaacagcatt ttttccatcg
    81421 tcatcgttat tccaacagtc ggcacaagat tggctttagg ccacttgaag agcagttacc
    81481 tcagtggatt gaagaaagcc tgcagttttt aaaaaagaac actaaaaaga gtctggaacc
    81541 ttagtagttc caaacccttt tttatttcgt cttaattact ggcacatcat agagttcagt
    81601 cgataaatag cgttcgccat tatctggtaa aatagttaac acatgtttgc cttttcctaa
    81661 ttgtttagcg acttcaatag cggcgtaaag agcagctcca gaagaaatcc caaccaggaa
    81721 gccttcctta gctcctgtta gtcgagcagt ttctaaagca tctttcgatt taacacggat
    81781 aatttggtca taggctttgg tatctaacgt gtttgggata aatccagctg atataccttg
    81841 aattttatgt ggtccaggct cttgaccaga taagacagca gattcttcag cttcaacagc
    81901 atagataaca gtttcagggc tagctttttt caagacatgt gaaacaccag aaagtgttcc
    81961 tccagtacca acaccagata cgaatgcatc taaagaaatc tccttaaaag cttccaaaat
    82021 ttcttgagct gttgtttttt catggatgct tggattggca gggttattaa attgcatagg
    82081 catccaagca cctagttcta ttgctaaagt ttcagctttt gcaatagccc ctttcatacc
    82141 ttctgctcca ggtgttaaga caagctctgc accataagcc tgaatgattt gtcgtctttc
    82201 caagctcata gtttcgggca taacaataat gactcgatac cctttagcag cacctaccca
    82261 tgcaagacca atacctgtat taccgcttgt tggttcgata ataacgtcac caggacttat
    82321 cagaccttca gcttcagcag cttcaatcat cgataaagca atacgatctt taacagaaga
    82381 tcctgggtta aaagcttcta attttacata aacgtcagca gcttcgtttg gaattaaaca
    82441 gttaagtttg ataataggtg tttgacctac taattctgtt atagttttgt aaattttagt
    82501 catagtttag cctcttttct tgatacaaaa taaatacgct gtgtatttaa gtataaggag
    82561 agatattatt aaagtaaaat acagatttac tatcactctg ataataaagc catatcactt
    82621 ttgttgatga ttaatcacag gggaattcaa taactttgga tccgattttc tcacagctta
    82681 tctgaccatg ataataattt gttaaagtat caatgagatt ttccacacgt tcagtatcac
    82741 aataaagtgt tgtctttatc gtgtctgaaa acttagtttc tgtttcggtt aaagccaatt
    82801 gatctagcaa gttagagtat agttggtatt ggggataaga aagtgtgatt tctagtccta
    82861 tctgctgttt aacttcaatg atgccaaatc gtttaatagc tgttgcggtc atgttagagt
    82921 atgctctgat tagcccacct gttcctaatt taataccacc aaagtaacgc gttaccacaa
    82981 ctactacatt tgttaaacac tgcttttcta acacagaaag gataggtatt ccagcagttc
    83041 ctgaaggttc accatcatca cttgagcgct tgatctgact gttgttaccg ataatcatcg
    83101 caaagcagga atgattagct ttgtaatgtt cttttttgat agcattaaca aaattcttac
    83161 catcttcttc agtgcttact cgtttgatat gacaaatgaa acgagatttt ttaatacttt
    83221 cttcgaaaaa accactggct ttgattgtct taaaatgttc cataaaaaaa ttgtacaaga
    83281 aaagataaaa attagcaaaa aaaagcgaat aataaagtat ggagtctatt gaaaatagtt
    83341 atggacgatt gttgctagaa agccagttac cagattcagc aaagcagctt gctcaacctt
    83401 taaaaagtgt agtaatatcg agaggaaaaa tgatttgtca acgttgtcac tatcagcttg
    83461 atgaagaagc acgattgcca aatggagcct attattgccg attttgtctt gtctttggac
    83521 gaaatcaatc agataaattg ctctatgcta tcccacctat gcattttcca aaaggtaatt
    83581 atctggtttg gagcggtcaa ctaactgctt atcaagagat gatttcccaa caattattaa
    83641 tcaatatgca aaatcaaaaa acaacattag ttcatgctgt aactggtgca ggaaaaacag
    83701 aaatgattta tgctgcgatt gaggcggtaa ttaacacagg tggctgggtt tgcattgcca
    83761 gcccaagagt agatgtttgt gtagaggtgg ctactcgttt atcccaagcc ttctcctgca
    83821 gcatctgttt aatgcacgca gagtctcttc cttatcaaag agctcctatt atagtagcca
    83881 caacgcatca gctgctaaaa tttcacaagg cttttgatct cttaattatt gatgaagtag
    83941 acgcattccc ttttgtaaat aatattcaac ttcattatgc tgcaagtcag gctttaaaag
    84001 aaggtggcgc aaagatttta ttaactgcaa cttcaactag aactttagaa agaaaggtaa
    84061 acaagggaga agtagtcaag ctgacattgg cgagacgctt taataatagg ccattagtta
    84121 ttccgaaatt tataagaagt tttaatctat ttaaaatgat acatcggcaa aaacttcctc
    84181 ctaaaatttt aaaatactta aaaaaacaaa gaaaaacagg ctatcccctt ttaatttttc
    84241 tacccactat tataatggca gaatctgtta cagcaatctt aaaagagtta ttgcccgccg
    84301 aacaaatagc ttgtgtttct agtcaatcac aaaacaggaa agaagatatc acagcttttc
    84361 gtcaagggaa aaaaacgatt ttaattacaa caacaatttt agaaaggggt gtgacatttc
    84421 cccaaattga tgtttttgtt cttgggtctc atcatcgtgt ctatagttcc caaagtcttg
    84481 ttcaaattgc tggacgagtt ggaagatcta tagacagacc agacggaacg ttatatttct
    84541 ttcatgaagg tatcagtaaa gcaatgctac tagcaagaaa agagattaaa gaaatgaatt
    84601 ataaagggta ttcgcatgat ctgtctacta tgtcaacaaa ttagtcaaac accaataagt
    84661 attacagaaa tcatcttttt aaggcgtatc tcttcaccga tttgtcaaca atgtcaaaaa
    84721 agctttcaaa agataggaaa aagtgtttgt gcgacatgtt gtgcaaactc agatataata
    84781 gcttgtcgag attgtctaaa atgggaaaac aaaggataca atgtaaatca tagaagctta
    84841 tattgttata atgctgctat gaaagcatac ttcagtcaat ataagtttca aggagactat
    84901 ttattaagaa aagtttttgc agtagaactt gccgatgtta ttaccaagta ctataaaggc
    84961 tatatcccag tcccggttcc tgtaagtccc ggttgttttc gagaaagaca atttaatcaa
    85021 gtgagcgcta ttcttgaggc agctaatgtt agctaccttt ctctttttga aaagctagat
    85081 aatactcacc aatcttccag aacaaaaaaa gagagattat tagtagaaaa atcttatcga
    85141 ctactaaaag tatcaaacat tcctgataaa atccttatag tagatgatat ttatactact
    85201 ggtagtacaa ttatcgctct tagaaaacaa ttggctaaag tagcaaatag tgacattaaa
    85261 agtttgtcaa ttgcacgtta aaacttgcaa attttcatga aagcagtata atagtattaa
    85321 agataaagta agcgcttcaa gcgcgagaaa gaggtatcct atgattaagt tcagtattcg
    85381 tggagaaaat atcgaagtga cagaagctat tcgcgattat gtagaatcaa agcttactaa
    85441 aatcgaaaaa tattttgcta aagaccaaga aatagatgca cgtgttaatt taaaagttta
    85501 tcgggagaga tcttcaaaag ttgaggtaac gatcccatta gattcagtaa cccttcgtgc
    85561 tgaagatgtt tcacaggata tgtacggctc tattgattta gttgttgata agatcgaacg
    85621 ccaaattaga aaaaataaaa ccaaaattgc taaaaaacat cgcgaaaagg ttccaactgg
    85681 tcaagtcttt acaactgagt ttgaagctga agaagtcgat gagataccag aagtacaagt
    85741 tgtacgtact aaaaatgtga cattaaaacc aatggatgtt gaagaagcgc gtttacagat
    85801 ggaactattg ggacatgatt tctttatcta cactgattct gaagatggtg caacaaacat
    85861 tttataccgt cgcgaagatg gaaacctagg attaatagaa gctaaatagt tcacaactaa
    85921 aacctacagg gatgattata cgtattttaa aagtgggaaa aagctattca tgtttcgagg
    85981 tgacatgatg aaaatgagat taagtgatag atacgacagt cattatataa caaaagtatc
    86041 tattacagtt cctctttaag taaatcttac gtaaaattag tttgaaaaaa agattagtcc
    86101 caattctggg ctagaaattc tcaaaaaata tgaaaaagcg cgttttcaga atataaagcg
    86161 cgtttttttc tttttccatt taacaataaa aaactcatga cgaacatggt atcataagag
    86221 aatttttact t
//
LOCUS       DATXIG010000003        85205 bp    DNA     linear   BCT 28-DEC-2023
DEFINITION  TPA_asm: Streptococcus pyogenes strain MGAS11113
            SAMN08555799-rid19682583.denovo.003, whole genome shotgun sequence.
ACCESSION   DATXIG010000003 DATXIG010000000
VERSION     DATXIG010000003.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN08555799
            Sequence Read Archive: SRR6740495
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pyogenes
  ORGANISM  Streptococcus pyogenes
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 85205)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 85205)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-OCT-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-OCT-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001918058.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001918058.2
            Genome Coverage       :: 368x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/12/2023 11:23:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 1,806
            CDSs (total)                      :: 1,769
            Genes (coding)                    :: 1,722
            CDSs (with protein)               :: 1,722
            Genes (RNA)                       :: 37
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1, 1 (5S, 23S)
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 47
            CDSs (without protein)            :: 47
            Pseudo Genes (ambiguous residues) :: 0 of 47
            Pseudo Genes (frameshifted)       :: 30 of 47
            Pseudo Genes (incomplete)         :: 20 of 47
            Pseudo Genes (internal stop)      :: 9 of 47
            Pseudo Genes (multiple problems)  :: 11 of 47
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..85205
                     /organism="Streptococcus pyogenes"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN08555799-rid19682583.denovo.003"
                     /strain="MGAS11113"
                     /serotype="Emm28"
                     /isolation_source="normally sterile site"
                     /host="Homo sapiens"
                     /db_xref="taxon:1314"
                     /country="Finland"
                     /collection_date="2002"
                     /collected_by="National Public Health Laboratory"
     gene            517..1044
                     /locus_tag="VC122_000157"
     CDS             517..1044
                     /locus_tag="VC122_000157"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014612026.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YqeG family HAD IIIA-type phosphatase"
                     /protein_id="HEP5640115.1"
                     /translation="MSIDDYRPTYMVEAIYDLRANDLLRHGITAVLVDLDNTLIAWNN
                     PDGTPEVRAWLDEMTIADISVVVVSNNKHSRVERAVSRFGVDFISRALKPFAYGIEKA
                     IARYGFDRNEVIMVGDQLMTDIRASHRAGIKSVLVKPLVASDAWNTKINRWRERRVMA
                     KLEEKYGKLSYQKGI"
     gene            1044..2162
                     /gene="yqeH"
                     /locus_tag="VC122_000158"
     CDS             1044..2162
                     /gene="yqeH"
                     /locus_tag="VC122_000158"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018363769.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome biogenesis GTPase YqeH"
                     /protein_id="HEP5640116.1"
                     /translation="MEELFCIGCGIQIQTEDKEKAGFTPAAALKKGMETGELYCQRCF
                     RLRHYNEITDVHITDDEFLRLLHEVGDSDALVVNVIDIFDFNGSIIPGLSRFISGNDV
                     LLVGNKKDILPKSVKDGKVTQWLTERAHEEGLRPLDVMLTSAQNKYAIKDLIERINEL
                     RNGRDVYVVGVTNVGKSTLINAIIQEITGDKDVITTSRFPGTTLDKIEIPLDDGSFIF
                     DTPGIIHRHQMAHYLSPKELKIVSPKKEIKPKTYQLNPEQTLFLGGLSRFDFINGERQ
                     GFTAFFDNQLELHRTKLAGADAFYDKHVGTLLTPPGKKELTAFPKLVRHEFTIDQKMD
                     IVFSGLGWIRVNGQKDSKAIVAAWAPEGVAVIVRKAII"
     gene            2188..2496
                     /gene="yhbY"
                     /locus_tag="VC122_000159"
     CDS             2188..2496
                     /gene="yhbY"
                     /locus_tag="VC122_000159"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003101715.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome assembly RNA-binding protein YhbY"
                     /protein_id="HEP5640117.1"
                     /translation="MLTSKQRAFLKSEAHSLKPIVQIGKNGLNDHIKTSIRQALDARE
                     LIKVTLLQNTDEDIHEVAEILEEEIGCDTVLKIGRILILYKVSAKKENRKLSPKVKAI
                     "
     gene            2565..3197
                     /locus_tag="VC122_000160"
     CDS             2565..3197
                     /locus_tag="VC122_000160"
                     /EC_number="2.7.7.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002313156.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nicotinate-nucleotide adenylyltransferase"
                     /protein_id="HEP5640118.1"
                     /translation="MALELLTPFTKVELEEEKKESNRKQIGILGGNFNPIHNAHLVVA
                     DQVRQQLGLDQVLLMPECKPPHVDAKETIDEKHRLRMLELAIEDVEGLAIETCELERQ
                     GISYTYDTMLYLTEQHPDVDFYFIIGADMVDYLPKWHRIDELVKLVQFVGVQRPKYKA
                     GTSYPVIWVDLPLMDISSSMIRDFIKKGRQPNYLLPKRVLDYITQEGLYQ"
     gene            3194..3787
                     /gene="yqeK"
                     /locus_tag="VC122_000161"
     CDS             3194..3787
                     /gene="yqeK"
                     /locus_tag="VC122_000161"
                     /EC_number="3.6.1.41"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012560451.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bis(5'-nucleosyl)-tetraphosphatase (symmetrical)
                     YqeK"
                     /protein_id="HEP5640119.1"
                     /translation="MTYEDYLPYSRTELLAKIAEQMSPKRFKHVLGVEKAALSLAECY
                     GCNPDKAGLAALLHDYAKECPDQVFLDLIDKYQLSPELAKWNNNVWHGMVGIYKIQED
                     LGLKDKDILRAIEIHTVGAAEMTLLDKVLYVADYIEEGRIFPLVDDARKIAKLDLNQA
                     VAYETVNTVAYLASKAQPIFPQTLDTYNAFCSYLKEY"
     gene            3811..4164
                     /gene="rsfS"
                     /locus_tag="VC122_000162"
     CDS             3811..4164
                     /gene="rsfS"
                     /locus_tag="VC122_000162"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003049699.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome silencing factor"
                     /protein_id="HEP5640120.1"
                     /translation="MKKEELLKIVVEATDEKRAKDILALDLEGLTSLTDYFVIASATN
                     SRQLEAIADNIREKVKEAGGDASHVEGNSQAGWVLLDLTDVVVHLFLEDERYHYNLEK
                     LWYEAPAVALDAYLA"
     gene            4212..4955
                     /locus_tag="VC122_000163"
     CDS             4212..4955
                     /locus_tag="VC122_000163"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014407307.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HEP5640121.1"
                     /translation="MKHNYEKFASVYDAVMDDSLYDLWTDFSLRHLPKSKGRNRLLEL
                     ACGTGIQSVRFAQAGFDVTGLDLSQDMLAIAKKRAQSAKKKIDFIQGNMLDLSQVGQF
                     DFVTCYSDSICYMQDEVDVGDVFKEVYDVLANDGIFIFDVHSTYQTDECFPGYSYHEN
                     ADDFAMVWDTYADEAPHSVVHELTFFIQEDDGRFSRFDEVHEERTYELLTYDILLEQA
                     GFKSFKVYADFEDKEPTKTSKRWFFVAYK"
     gene            5209..6315
                     /locus_tag="VC122_000164"
     CDS             5209..6315
                     /locus_tag="VC122_000164"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014407308.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotidyltransferase"
                     /protein_id="HEP5640122.1"
                     /translation="MTVTGIIAEFNPFHNGHKYLLETAEGLKIIAMSGNFMQRGEPAL
                     IDKWIRSEMALKNGADIVVELPFFVSVQSADYFAQGAIDILCQLGIQQLAFGTEDVID
                     YQKLIKVYEKKSKQMTAYLSTLEDTLSYPQKTQKMWEIFAGVKFSGQTPNHILGLSYA
                     KASAGKHIQLCPIKRQGAAYHSKDKNHLLASASAIRQHLNDWDFISHSVPNAGLLINN
                     PHMSWDHYFSFLKYQILNHSDLTSIFQVNDELASRIKKAIKVSQNIDHLVDTVATKRY
                     TKARVRRILTYILVNAKEPTLPKGIHILGFTSKGQAHLKKLKKSRPLITRIGAETWDE
                     MTQKADSIYQLGHQDIPEQSFGRIPIIIKNERLN"
     gene            6797..7513
                     /locus_tag="VC122_000165"
     CDS             6797..7513
                     /locus_tag="VC122_000165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008088325.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YebC/PmpR family DNA-binding transcriptional
                     regulator"
                     /protein_id="HEP5640123.1"
                     /translation="MGRKWANIVAKKTAKDGATSKVYAKFGVEIYVAAKQGEPDPELN
                     TALKFVIDRAKQAQVPKHVIDKAIDKAKGNTDETFVEGRYEGFGPNGSMIIVDTLTSN
                     VNRTAANVRTAYGKNGGNMGASGSVSYLFDKKGVIVFAGDDADSVFEQLLEADVDVDD
                     VEAEEGTITVYTAPTDLHKGIQALRDNGVEEFQVTELEMIPQSEVVLEGDDLETFEKL
                     IDALESDDDVQKVYHNVADF"
     gene            7716..8558
                     /locus_tag="VC122_000166"
     CDS             7716..8558
                     /locus_tag="VC122_000166"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012766588.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter substrate-binding
                     protein"
                     /protein_id="HEP5640124.1"
                     /translation="MIKKQYLGRATIALASTLVLAACGSSKTAESGNQGSSKEVLFAT
                     VGTTAPFSYEKGGQLTGYDIEVAKAVFKGSDDYRVSFKKTEWSSIFTGLDSGKYQMGG
                     NNISFTKERSAKYLFSYPIGSTPSVLVVPKDSDIKSFDDIQGHTTQVVQGTTSVAQLE
                     DFNKKHSDNPVTLKFTNENITQMLTNLSEGKADFKIFDAPTVNAIIKNQGLDNLKTIE
                     LTSTEQPFIYFIFSQDQEKLQSFVNKRIKELTADGTLSKLAKEHLGGDYVPSDKELKL
                     PTAN"
     gene            8885..9730
                     /locus_tag="VC122_000167"
     CDS             8885..9730
                     /locus_tag="VC122_000167"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002985973.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MetQ/NlpA family ABC transporter
                     substrate-binding protein"
                     /protein_id="HEP5640125.1"
                     /translation="MSFKKWIGIVGIALASTVLVACSEKQDDKNTLTIGVMTKTESDQ
                     ARWDKVEELLKKDNITLKYKEFTDYSQPNKAVANGEVDINAFQHYNFLNNWNKENKEH
                     LVAIADTYISPINLFSGTSQDGKAKYKSVADLPNGAQIAVPNDATNESRALYVLQSAG
                     LIKLNVSGDQLATIANISENKKKLDIKELDASQTARALVSADAAVVNNSYAVPAKIDY
                     KTSLFKEKADDNSKQWINIIAGQKDWEKSEKADAIKKLIKAYQTDEVKKVVEKTSNGI
                     DVSVW"
     gene            9980..11044
                     /locus_tag="VC122_000168"
     CDS             9980..11044
                     /locus_tag="VC122_000168"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011184185.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methionine ABC transporter ATP-binding protein"
                     /protein_id="HEP5640126.1"
                     /translation="MNEAIIQLDHIDITFRQKKRVIEAVKDVTVHINQGDIYGIVGYS
                     GAGKSTLVRVINLLQAPTNGKITVDGDVTFDQGKIQLSADALRQKRRDIGMIFQHFNL
                     MAQKTAKENVAFALRHSSLSKTEKEHKVIELLELVGLSERADNYPAQLSGGQKQRVAI
                     ARALANDPKILISDEATSALDPKTTKQILALLQELNRKLGLTIVMITHEMQIVKDICN
                     RVAVMQNGVLIEEGSVLDIFSNPKEALTQEFITTATGIDEALEKINQQDIVKHLPANA
                     LLAQLKYAGTSTDEPLLNSIYRQFEVTANILYGNIEILDHIPVGDMIVVLEGQAENIL
                     AAEKALHEAGVDVSILKRGA"
     gene            11045..11737
                     /locus_tag="VC122_000169"
     CDS             11045..11737
                     /locus_tag="VC122_000169"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002991072.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEP5640127.1"
                     /translation="MSQLIQTYLPNVYELGWSGDAGWGLAIWNTLYMTIVPFIVGGAI
                     GLLLGLLLVLTGPDGVIENKTICWVIDKVTSIFRAIPFVILIAILASFTYLLLRTTLG
                     ATAALVPLTFATFPFYARQVQVVFSELDKGVIEAAQASGATFWDIVKVYLSEGLPDLI
                     RVSTVTLISLVGETAMAGAIGAGGLGNVAISYGYNRFNNDVTWVATIIILLIIFAIQF
                     IGDSLTRRFSHK"
     gene            complement(11791..13161)
                     /gene="brnQ"
                     /locus_tag="VC122_000170"
     CDS             complement(11791..13161)
                     /gene="brnQ"
                     /locus_tag="VC122_000170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002985966.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid transport system II
                     carrier protein"
                     /protein_id="HEP5640128.1"
                     /translation="MKQKNVYIVIGFMLFALFFGAANLIYPAFLGIYSGHQILWSIIG
                     FCLTGVSLPLLGVIAVAKSGSGDVESLARPISKWYAIFYSSILYLSIGPFFAIPRTGA
                     TSFSVGIAPILGDNTTNKAIYAILFFGLSYFLAIKPSKLAENIGKFLTPTLLVVISIL
                     VIASFVHPAGNYGDAFNAGVGVNNAFKDFPFIAGLIQGYGTMDALASLVFAILVIEAT
                     KQFGAKTDKEVTKITLISGAIAILLLALVYIFVGRIGATSQSLFPFSDGSFTLHGNPV
                     NGGQILSHASRFYLGGIGQAFLAVVIFLACLTTSTGLITSSAEYFHKLVPALSHIAWA
                     TIFTLLSAFFYFGGLSVIINWSAPVLFLLYPLTVDLIFLVLAQKCFNNDPVVYRTTIG
                     LTFIPAIFDALLTLSQMTGLFHLPEAVVTFFQKTVPLGQFSMGWIIFAAIGFLIGLIL
                     SKTKKS"
     gene            13395..14609
                     /gene="sstT"
                     /locus_tag="VC122_000171"
     CDS             13395..14609
                     /gene="sstT"
                     /locus_tag="VC122_000171"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012516260.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine/threonine transporter SstT"
                     /protein_id="HEP5640129.1"
                     /translation="MKKIYDLWVRVSLIKKIGIGVVIGVMLGILAPDLTGFSILGKLF
                     VGGLKAIAPLLVFALVSQAISHQKKGKQTNMTLIIVLYLFGTFASALVAVLTAYLFPL
                     TLVLNTPVNTELSPPQGVAEVFQSLLLKLVDNPINALATANYIGVLSWAIIFGLALKA
                     ASQETKHLIKTAAEVTSQIVVWIINLAPIGIMSLVFTTISENGVGILSDYAFLILVLV
                     GTMVFVALVVNPLIAVLITRQNPYPLVLRCLRESGLTAFFTRSSAANIPVNMQLCQKI
                     GLSKDTYSVSIPLGATINMGGAAITINVLTLAAVHTFGIPIDFLTALLLSVVAAVSAC
                     GASGVAGGSLLLIPVACSLFGISNDLAMQVVSVGFIVGVIQDSCETALNSSTDVLFTA
                     IAENAFWKRKKA"
     gene            complement(14664..15338)
                     /locus_tag="VC122_000172"
     CDS             complement(14664..15338)
                     /locus_tag="VC122_000172"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002985961.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TrkA family potassium uptake protein"
                     /protein_id="HEP5640130.1"
                     /translation="MLKRKTVGVLGLGIFGRTVARELSNFDQDVIAIDIRESHVKEVA
                     DLVTKAAVGDITDKEFLLAVGIEHCDTVVIASGNNLESSVLAVMHCKKLGVPTIIAKA
                     KNKIFEEVLYGIGATKVITPERDSGKRVASNLLRRHIESIIYLEHGISMIEFVIPKSW
                     EGQSLSELDVRRKYELNVIGMRQKEVKTLDTNVKPFEPLEPNTIIVAIANDHTFEKFD
                     YLGYLK"
     gene            complement(15348..16739)
                     /locus_tag="VC122_000173"
     CDS             complement(15348..16739)
                     /locus_tag="VC122_000173"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014407312.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TrkH family potassium uptake protein"
                     /protein_id="HEP5640131.1"
                     /translation="MSGGNMKRSFIKSLSVTQRLTFSFAIVILIGTLLLSMPFTHYQN
                     GPNTVYLDHFFNVVSMVCVTGLSVVPVAEVYNGIGQTIAMALMQIGGLGLVTLIAVST
                     FALKRKMRLSDQTLLQSALNRGDSKDLKHYLFFAYKVTFSIEAFAAIVIMIDFIPRFG
                     WKNGIFNSIFLAVSAFCNAGFDNLGSSSLKDFMLNPTLNVIITFLIISGGLGFAVWVD
                     LGVAFKKYFFERPHCYGATFRKLSNQSRLVLQTTAVILFLGTFLTWFLEKDNSKTIAN
                     FSLHQQLMVSFFQTVTMRTAGFATISYNDTLAPTNILYMIQMVIGGAPGGTAGGIKVT
                     TAAITFLLFKAELSGQSEVTFRNRIIANKTIKQTMTVLIFFFAVLMIGFILLLSVEPH
                     IAPIPLLFESISAIATVGVSMDLTPQLSTAGRLIVIVLMFVGRVGPITVLISLIQRKE
                     KTIQYATTDILVG"
     gene            complement(16809..17522)
                     /gene="rsmG"
                     /locus_tag="VC122_000174"
     CDS             complement(16809..17522)
                     /gene="rsmG"
                     /locus_tag="VC122_000174"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002991845.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (guanine(527)-N(7))-methyltransferase
                     RsmG"
                     /protein_id="HEP5640132.1"
                     /translation="MTPQDFYRTLEEDGFSLSSKQKEQFDTYFKLLVEWNTKINLTAI
                     TEENEVYLKHFYDSIAPILQGFLANEPIKLLDIGAGAGFPSLPMKILFPNLEVTIIDS
                     LNKRISFLTLLAQELGLENVHFFHGRAEDFGQDKAFRGQFDVVTARAVARMQVLSELT
                     IPFLKIGGKLIALKAQAADQELEEAKNALCLLFGKVIKNHSYQLPNGDSRFITIVEKK
                     KETPNKYPRKAGLPNKKPL"
     gene            17672..18229
                     /locus_tag="VC122_000175"
     CDS             17672..18229
                     /locus_tag="VC122_000175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014407314.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LemA family protein"
                     /protein_id="HEP5640133.1"
                     /translation="MPTVLIILVVLGVLALWLMISYNSLVKSRMHTKEAWSQIDVQLK
                     RRNDLIPNLIETVKGYASYEQKTFEKITDLRARVANASTPQETMAASNELSKQVTSLF
                     AVAENYPDLKANENFLKLQEELTNTENKISYSRQLYNSTTSNYNLQLESFPSNIAGKL
                     FGFKPSEFLQTPEAEKEVPKVEFNF"
     gene            18276..19172
                     /gene="htpX"
                     /locus_tag="VC122_000176"
     CDS             18276..19172
                     /gene="htpX"
                     /locus_tag="VC122_000176"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012560458.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc metalloprotease HtpX"
                     /protein_id="HEP5640134.1"
                     /translation="MLYQQISQNKQRTVVLLVVFFALLALIGASAGYLLLDNYAMGLV
                     LALVIGVIYATSMIFQSTSLVMSMNNAREVTEKEAPGFFHIVEDMAMVAQIPMPRVFI
                     IEDPSLNAFATGSSPQNAAVAATTGLLEVMNREELEGVIGHEISHIRNYDIRISTIAV
                     ALASAVTVISSIGGRMLWYGGGSRRQRDDGDDDVLRIITLLLSLLSLLLAPLVASLIQ
                     LAISRQREYLADASSVELTRNPQGMINALEKLQLSQPMKHPVDDASAALYINEPRKKR
                     SFSSLFSTHPPIEERIERLKNM"
     gene            19406..19939
                     /locus_tag="VC122_000177"
     CDS             19406..19939
                     /locus_tag="VC122_000177"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011017345.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF177 domain-containing protein"
                     /protein_id="HEP5640135.1"
                     /translation="MLAISEIRKHPDGLSFDRLCDVKSMLLERDQQIIDIKAVKAVGN
                     VRYDKGLYLLDYQLSYEVILPSSRSMVPVCLSEVQHIQELFIEATDLADKKELVEDNL
                     VLVLDKDAINLEESIVDNILLAIPLQVLTEEEKKSKELPAGQNWAVLTEEDYQCLKEE
                     KQKENNPFASLQGLFDE"
     gene            20206..20892
                     /gene="covR"
                     /locus_tag="VC122_000178"
                     /gene_synonym="csrR"
     CDS             20206..20892
                     /gene="covR"
                     /locus_tag="VC122_000178"
                     /gene_synonym="csrR"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002991052.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system response regulator CovR"
                     /protein_id="HEP5640136.1"
                     /translation="MTKKILIIEDEKNLARFVSLELQHEGYEVIVEVNGREGLETALE
                     KEFDLILLDLMLPEMDGFEVTRRLQTEKTTYIMMMTARDSIMDVVAGLDRGADDYIVK
                     PFAIEELLARVRAIFRRQDIESEKKVPSQGIYRDLVLNPQNRSVNRGDDEISLTKREY
                     DLLNILMTNMNRVMTREELLSNVWKYDEAVETNVVDVYIRYLRGKIDIPGKESYIQTV
                     RGMGYVIREK"
     gene            20898..22400
                     /gene="covS"
                     /locus_tag="VC122_000179"
                     /gene_synonym="csrS"
     CDS             20898..22400
                     /gene="covS"
                     /locus_tag="VC122_000179"
                     /gene_synonym="csrS"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002991036.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system sensor histidine kinase
                     CovS"
                     /protein_id="HEP5640137.1"
                     /translation="MENQKQKQKKYKNSLPKRLSNIFFVLFFCIFSAFTLIAYSSTNY
                     FLLKKEKQSVFQAVNIVRVRLSEVDSNFTLENLAEVLYKNDKTHLRIDDRKGSRVIRS
                     ERDITNTLDANQDIYVYNIDKQMIFTTDNEESSPGLHGPIGRVYHDHIEDQYRGFSMT
                     QKVYSNRTGKFVGYVQVFHDLGNYYVIRARLLFWLLVVELFGTSLAYLIILITTRRFL
                     KPLHNLHGVMRNISENPNNLNLRSDISSGDEIEELSVIFDNMLDKLETHTKLQSRFIS
                     DVSHELRTPVAIIKGHIGLLQRWGKDDSDILEESLTATAHEADRMAIMINDMLDMIRV
                     QGSFEGHQNDMTVLEDSIETVVGNFRVLREDFIFTWQSENPKTIARIYKNHFEQALMI
                     LIDNAVKYSRKEKKIAINLSVTGKQEAIVRVQDKGEGISKEDIEHIFERFYRTDKSRN
                     RTSTQAGLGIGLSILKQIVDGYHLQMKVESELNEGSVFILHIPLAQSKES"
     gene            22615..23109
                     /gene="nrdR"
                     /locus_tag="VC122_000180"
     CDS             22615..23109
                     /gene="nrdR"
                     /locus_tag="VC122_000180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012657916.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional repressor NrdR"
                     /protein_id="HEP5640138.1"
                     /translation="MRCPKCNYHKSSVVDSRQAEDGNTIRRRRECEQCHTRFTTFERV
                     EELPLLVIKKDGTREQFSRDKILNGVVQSAQKRPVSSTDIENVISRIEQEVRTTYENE
                     VSSTAIGNLVMDELAELDEITYVRFASVYKSFKDVDEIEELLQQITNRVRGKKKRLNN
                     DETN"
     gene            23093..24268
                     /locus_tag="VC122_000181"
     CDS             23093..24268
                     /locus_tag="VC122_000181"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002991019.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation and membrane attachment
                     family protein"
                     /protein_id="HEP5640139.1"
                     /translation="MMKPIDTFTYLKRNKVTLDSVTLIQLYFPIIGSDAVSIYQYFIH
                     FFDDGLQRHKFSDILNHLQFGMKRFEDALAILTAMELVSVYQLSDTYLITLHQPLSRD
                     LFFQHPAYSRLLEQKIGEVAVSELQVTVPSQARNISKRFSDIFGVQGDLTNVPQKPQK
                     NFDLSSFQQLMVRDGLQFEDNQKDIISLYSIAEQYDMTWFDTYQIAKATAVNGKIRPE
                     RLLAKKNQSMTKPSKENFSQAEQIILREAKQDSALVFLEKIKKARRATITKDERILLQ
                     TLAKMNFLDDVINVMVLYTFNKTKSANLQKSYVLKMANDFAYQKVSTAEEAIVVLRAF
                     TDRQSRRQSKVKTSQSNVPKWSNPDYQETTSQEEQAKLDQFKQAALKRLENLGKGGD"
     gene            24269..25171
                     /gene="dnaI"
                     /locus_tag="VC122_000182"
     CDS             24269..25171
                     /gene="dnaI"
                     /locus_tag="VC122_000182"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014612041.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="primosomal protein DnaI"
                     /protein_id="HEP5640140.1"
                     /translation="MEKIGETMAKLGQNTRVNSDQLIQTILADPEVASFISQHHLSQE
                     QINLSLSKFNQFLVERQKYQLKDPSYIAKGYQPILAMNEGYADVSYLETKELVEAQKQ
                     AAISERIQLVSLPKSYRHIHLPDIDVNNASRMEAFSAILDFVEQYPSAEQKGLYLYGD
                     MGIGKSYLLAAMAHELSEKKGVSTTLLHFPSFAIDVKNAISNGSVKEEIDAVKNVPVL
                     ILDDIGAEQATSWVRDEVLQVILQYRMLEELPTFFTSNYSFADLERKWATIKGSDETW
                     QAKRVMERVRYLAREFHLEGANRR"
     gene            25234..26544
                     /gene="der"
                     /locus_tag="VC122_000183"
     CDS             25234..26544
                     /gene="der"
                     /locus_tag="VC122_000183"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018377046.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome biogenesis GTPase Der"
                     /protein_id="HEP5640141.1"
                     /translation="MVLPTVAIVGRPNVGKSTLFNRIAGERISIVEDVEGVTRDRIYA
                     TGEWLNRQFSLIDTGGIDDVDAPFMEQIKHQAQIAMEEADVIVFVVSGKEGVTDADEY
                     VSKILYRTNTPVILAVNKVDNPEMRNDIYDFYSLGLGDPYPVSSVHGIGTGDVLDEIV
                     ENLPVEEAEENDDIIRFSLIGRPNVGKSSLINAILGEDRVIASPVAGTTRDAIDTHFT
                     DADGQEFTMIDTAGMRKSGKIYENTEKYSVMRAMRAIDRSDVVLMVINAEEGIREYDK
                     RIAGFAHEAGKGMIIVVNKWDTIDKDNHTVAKWEADIRDQFQFLTYAPIIFVSALTKQ
                     RLNKLPDLIKRISESQNKRIPSAVLNDVIMDAIAINPTPTDKGKRLKIFYATQVSVKP
                     PTFVVFVNEEELMHFSYLRFLENQIRAAFTFEGTPIHLIARKRK"
     gene            26751..29849
                     /locus_tag="VC122_000184"
     CDS             26751..29849
                     /locus_tag="VC122_000184"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002992435.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase"
                     /protein_id="HEP5640142.1"
                     /translation="MMARLIPGRVRNEGIKLYEQGLVSFQDDNKGILQIEVETYQVQY
                     GADDEDITCQCDTFHMKHYCKHIAAVEYFLKNDQKGKLFLKQLTNQTKIKETTKKMTS
                     FGSLFLDSLAMNEDDSVKYRLSALGSRSPFSSDYWWSLKINRLPDDRSYVIRDIKGFL
                     QLIKKEGFYQIGKNYFEQLSWLQFDPSSQDLIEFLWRLASNTDKGDNENIFPNHARHL
                     RLPSGFFEEGIHYLTSLYDFTFEGPSQTYHHLFVRSLEAEAGLYEFKVEVHRKSIELQ
                     IAEKNVQYLFDNDYLLYQDTFYHLTLKQRKMVQAIRSLPIEADLAKHIHFDLDDHAKL
                     AASLSDFKQIGLVEAPKSFAIRDFEVTFQFDLLNRDEISCQLMFDYGNYQVSDKASLE
                     ALPFASHLKKEEKINRSLLAFGFSPQFYSKKRLTSAKELYTFFEETVPCFERLGNVAL
                     STAIQALQVKEMPKIAIRRNQGLLDISFDFSTIIENDIDQAVTALFQNNPYFVSQTGQ
                     LVVFDDETQKVSKSLQELRARQLKNGHLQLDGIRALQVSKLFEGMTSVHFSKELEELA
                     YHLQHPETFSIKPLPVKAQMRDYQRNGVQWLSMLNHYGFGGILADDMGLGKTLQTLAF
                     LASHLKSDSKVLILSPSSLIYNWFDECQKFTPQLDVVVSYGLKQIRDQIIEEGHQITI
                     TSYSSFRQDFETYQAFHYDYLILDEAQVIKNAQTKISHCLRAFNTANCFALSGTPIEN
                     KMLEIWSIFQIVLPGLLPTKKEFLKLTAEQVSRYIKPFVMRRKKEDVLPELPDLIETN
                     YSNEMTDEQKAIYLAQLRQMKDQIRNSSDVDISRQKIEILSGITRLRQICDTPSLFMD
                     YQGKSGKLDSLRILLTQIKENGHRALIFSQFRGMLDLAKQEMTALGLTSYQMTGSTPA
                     NERQEMTRAFNNGSKDAFLISLKAGGVGINLTGADTVILIDLWWNPAVEMQAISRAYR
                     IGQKENVEVYRLITRGTIEEKILELQESKRNLVTTVLDGNENRASMSIEEIKEILGLN
                     TH"
     gene            30092..30694
                     /locus_tag="VC122_000185"
     CDS             30092..30694
                     /locus_tag="VC122_000185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002985931.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEP5640143.1"
                     /translation="MVKNQFPLVADGIAISDPAKQMALYENEDLITNIRGYYQDKEYD
                     DIARNEEFTAKATSRQAPSSKRFCSNDEKHHYVKEARQKAKQDLKEKRQAYLAKEMAY
                     VPKQVSKKQQPADSSPSQKQATTEMSRFTKKLHQDNYILAELPKEYKEPKNLPQQGTT
                     KKNNYDFLKSSQIYNNKEMRQQREKTIAQELNLSRFEDLP"
     gene            30734..32062
                     /locus_tag="VC122_000186"
     CDS             30734..32062
                     /locus_tag="VC122_000186"
                     /EC_number="6.3.2.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018363778.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramate--L-alanine ligase"
                     /protein_id="HEP5640144.1"
                     /translation="MSKTYHFIGIKGSGMSALALMLHQMGHKVQGSDVEKYYFTQRGL
                     EQAGITILPFSEDNITPDMELIVGNAFRENNKEVAYALRHQIPFKRYHDFLGDFMKSF
                     ISFAVAGAHGKTSTTGLLSHVLKNITDTSYLIGDGTGRGSANAQYFVFESDEYERHFM
                     PYHPEYSIITNIDFDHPDYFTGIADVRNAFNDYAKQVKKALFVYGEDDELKKIEAPAP
                     IYYYGFEEGNDFIAYDITRTTNGSDFKVKHQGEVIGQFHVPAYGKHNILNATAVIANL
                     FVAGIDTALVADHLKTFSGVKRRFTEKIINDTIIIDDFAHHPTEIVATIDAARQKYPS
                     KEIVAIFQPHTFTRTIALLEDFACALNEADSVYLAQIYGSAREVDKGEVKVEDLAAKI
                     IKPSQVVTVENVSPLLDHDNAVYVFMGAGDIQLYEHSFEELLANLTKNNQ"
     gene            32108..32590
                     /locus_tag="VC122_000187"
     CDS             32108..32590
                     /locus_tag="VC122_000187"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002992439.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HEP5640145.1"
                     /translation="MLIRQVQGSDLEVIATIESDNFSPQEATTRAVLEERIRLIPDTF
                     LVALIDQEIVGYIEGPVVTTPILEDSLFHGVTKNPKTGGYIAITSLSIAKHFQQQGVG
                     TALLAALKDLVVAQQRTGLILTCHDYLISYYEMNGFINQGISESQHGGTLWYQMIWKL
                     "
     gene            32702..34276
                     /gene="mltG"
                     /locus_tag="VC122_000188"
     CDS             32702..34276
                     /gene="mltG"
                     /locus_tag="VC122_000188"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009880404.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="endolytic transglycosylase MltG"
                     /protein_id="HEP5640146.1"
                     /translation="MALTDFKDKDQQDQQRSFKEQILAELEKANQIRKEKEEELFQKE
                     LEAKEAARRTAQLYAEYKRQDAFQKESIAHNNKTAKHFQAIKGAVMTSEALKPTLLSE
                     KENSSLKTTNKRVVQANELQETASKESQVPLTIEKGHSVRRKLSKRQQTERAAKKIST
                     VLISSIIITLLAVTLAGAGYVYSALNPVDKNSDAFVQVEIPSGSGNKLIGQILQKKGL
                     IKNSTVFSFYTKFKNFTNFQSGYYNLQKSMSLEEIASALQEGGTAEPTKPSLGKILIP
                     EGYTIKQIAKAVEHNSKGKTKKAKTPFNEKDFLDLVTDEAFIQDMVKRYPKLLATIPT
                     KEKAIYRLEGYLFPATYNYYKETTMRELVEDMLAAMDATLVPYYDKIAASGKTVNEVL
                     TLASLVEKEGSTDDDRRQIASVFYNRLNSGMALQSNIAILYAMGKLGEKTTLAEDATI
                     DTTINSPYNIYTNTGLMPGPVASSGVSAIEATLNPASTDYLYFVANVHTGEVYYAKTF
                     EEHSANVEKYVNSQIQ"
     gene            34349..34831
                     /gene="greA"
                     /locus_tag="VC122_000189"
     CDS             34349..34831
                     /gene="greA"
                     /locus_tag="VC122_000189"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016481121.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription elongation factor GreA"
                     /protein_id="HEP5640147.1"
                     /translation="MAEKTYPMTLTEKEQLEKELEELKLVRRPEIVERIKIARSYGDL
                     SENSEYDAAKDEQAFVEGQISTLETKIRYAEIIDSDAVAKDEVAIGKTVIVQEVGTTD
                     KDTYHIVGAAGADIFSGKISNESPIAQALIGKKTGDKVRIESPAATYDVEIISVEKTN
                     "
     gene            complement(35451..36374)
                     /gene="yidC"
                     /locus_tag="VC122_000190"
     CDS             complement(35451..36374)
                     /gene="yidC"
                     /locus_tag="VC122_000190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015016705.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="membrane protein insertase YidC"
                     /protein_id="HEP5640148.1"
                     /translation="MKLTLNRILFSGLALSILFTLTGCVGRDAHGNPKGMIWEFLGKP
                     MSYFIDYFANNAGLGYGLAIIIVTIIVRTLILPLGLYQSWKASYQSEKMAFLKPVFEP
                     INKRIKQASSQEEKMAAQTELMAAQRAHGINPLGGIGCLPLLIQMPFFSAMYFAAQYT
                     KGVSTSTFMGIDLGSRSLVLTAIIAALYFFQSWLSMMAVSEEQREQMKTMMYTMPIMM
                     IFMSFSLPAGVGLYWLVGGFFSIIQQLITTYLLKPRLHKQIKEEYAKNPPKAYQSTSS
                     RKDVTPSQNMEQANLPKKIKSNRNAGKQRKR"
     gene            complement(36456..36734)
                     /locus_tag="VC122_000191"
     CDS             complement(36456..36734)
                     /locus_tag="VC122_000191"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010921912.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acylphosphatase"
                     /protein_id="HEP5640149.1"
                     /translation="MQKVRLIVSGRVQGVGFRYATHTLALDIGDIYGRVWNNNDGTVE
                     ILAQSKDSDKIATFIQEVRKGPSKWAKVTYVDVTMANFEDFQDFQIGK"
     gene            36995..37732
                     /locus_tag="VC122_000192"
     CDS             36995..37732
                     /locus_tag="VC122_000192"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011017403.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA methyltransferase"
                     /protein_id="HEP5640150.1"
                     /translation="MVITSKTNQLIKQTKKLLQKKHRKQSYLIEGWHLFEEAQKSGQV
                     FRHIFVLEEMVERLAGEQELVIVSPQVLKELTDSPSPQGIVAEVEIPKLAFPSDYKGK
                     YLVLEDVQDPGNLGTIIRTADAARFDGVFLSEKSADIYNQKTLRSMQGSHFHLPIWRT
                     DVYQLCRELQEYKTPILATTLSKKSVDYKSLTHHERLALVLGNEGQGISAEMAALADQ
                     LVHITMPGQAESLNVAVAAGILIFSLI"
     gene            37771..38271
                     /locus_tag="VC122_000193"
     CDS             37771..38271
                     /locus_tag="VC122_000193"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011017404.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HD domain-containing protein"
                     /protein_id="HEP5640151.1"
                     /translation="MHYTEDKEYMEHVGHLIAHPRFQKLSHIVQHQHSTRLEHSINVS
                     YSSYKLAKRFGWDAKSTARGGLLHDFFYYDWRVTKFNKGHAWVHPRIAVRNAKKLTEL
                     NKKEEDIILKHMWGATIAFPRYKESYIVTMVDKYWAVKEAVTPLRQKWSNRRFLRRKT
                     LQSHNR"
     gene            38286..38975
                     /locus_tag="VC122_000194"
     CDS             38286..38975
                     /locus_tag="VC122_000194"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003059008.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Bax inhibitor-1/YccA family protein"
                     /protein_id="HEP5640152.1"
                     /translation="MNDHVIYTQSDVGLNQFFAKIYSLVGMGVGLSAFVSYLMLYPFR
                     ENLISILVNQPMIYYGAAIIELILVFVASGAARKNTPAALPIFLIYSALNGFTLSFII
                     VAYAQTTVFQAFLSSAAVFFAMSIIGVKTKRDMSGLRKAMFAALIGVVVASLINLFIG
                     SGMMSYVISVISVLIFSGLIASDNQMIKRVYQATNGQVGDGWAVAMALSLYLDFINLF
                     ISLLRIFGRND"
     gene            39153..39395
                     /locus_tag="VC122_000195"
     CDS             39153..39395
                     /locus_tag="VC122_000195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003049770.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YneF family protein"
                     /protein_id="HEP5640153.1"
                     /translation="MSTAIWILLLIVALGVGVFGGIFIARKQIEKEIGEHPRLTPEAI
                     REMMSQMGQKPSEAKIQQTYRNIIKQSKAAVSKGKK"
     gene            39573..40367
                     /gene="racE"
                     /locus_tag="VC122_000196"
     CDS             39573..40367
                     /gene="racE"
                     /locus_tag="VC122_000196"
                     /EC_number="5.1.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003049772.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate racemase"
                     /protein_id="HEP5640154.1"
                     /translation="MDTRPIGFLDSGVGGLTVVCELIRQLPHEKIVYIGDSARAPYGP
                     RPKKQIKEYTWELVNFLLTQNVKMIVFACNTATAVAWEEVKAALDIPVLGVVLPGASA
                     AIKSTTKGQVGVIGTPMTVASDIYRKKIQLLAPSVQVRSLACPKFVPIVESNEMCSSI
                     AKKIVYDSLSPLVGKIDTLVLGCTHYPLLRPIIQNVMGPSVKLIDSGAECVRDISVLL
                     NYFDINGNYHQKAVKHRFFTTANPEIFQEIASIWLKQKINVEHVTL"
     gene            40364..41350
                     /locus_tag="VC122_000197"
     CDS             40364..41350
                     /locus_tag="VC122_000197"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011054210.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside-triphosphate diphosphatase"
                     /protein_id="HEP5640155.1"
                     /translation="MSEKIYEYKDENNWFIGKMTGHNLISGWGVKHTTIKKIDDLLDG
                     IAATLDWENPKGYDVSVVRYQSPLSLITFIIDMINQETQRKIKVTPHAGTILLMENAK
                     LLAVYLPEGGVSTATFFATSEQGFGDIILIATRNEGKTKEFRNLFGQLGYRVENLNDY
                     PELPEVAETGTTFEENARLKAETISRLTGKMVLADDSGLKVDALGGLPGVWSARFSGP
                     DATDAKNNAKLLHELAMVFDQKKRSAQFHTTLVVAAPNKDSLVVEAEWPGYIATQPKG
                     ENGFGYDPVFIVGETGRHAAELEADQKNQLSHRGQAVRKLMEVFPAWQAKQS"
     gene            41329..41850
                     /locus_tag="VC122_000198"
     CDS             41329..41850
                     /locus_tag="VC122_000198"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002985902.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metallophosphoesterase"
                     /protein_id="HEP5640156.1"
                     /translation="MASKTIIVMSDSHGDRDIVQAIKDKYLGQVDAIFHNGDSELNSS
                     DPIWAGIYVVGGNCDYDTGYPDRLVTQLGTVTIAQTHGHLYHINFTWDKLDYFAQEVA
                     ADICLYGHLHRPAAWQVGQTLFMNPGSVTQPRGEINEKLYARVELTDTQIKVDYFTRD
                     HKLYPSLSKEFKR"
     gene            41847..42308
                     /locus_tag="VC122_000199"
     CDS             41847..42308
                     /locus_tag="VC122_000199"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002985900.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CBS domain-containing protein"
                     /protein_id="HEP5640157.1"
                     /translation="MIAKEFETFLMSHLDNYLIPEQDLAIFIDTHNADHVMLLLVSNG
                     FSRVPVITREKKYVGTISISDIMMYQSKRQLTDWEMSQTDIGEMVNTKIETISITSSL
                     TEIMHKLIDFPFLPVVDRANRFVGIITRKSILKAVNSLLHDFTDDYTIIKK"
     gene            42305..43051
                     /gene="xerD"
                     /locus_tag="VC122_000200"
     CDS             42305..43051
                     /gene="xerD"
                     /locus_tag="VC122_000200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014635298.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific tyrosine recombinase XerD"
                     /protein_id="HEP5640158.1"
                     /translation="MKSYIEPFIASKALSQNSQKAYRYDLQQFCQLIGERVNQDKLLL
                     YQNSIANLSLSAKKRKLSTANQFLYYLYQIKYLNSYFRLTDTMKVMRTEKQQAAIINT
                     DIFYQKTPFVWGQLISLLILELGLTPSEVAGIEVANLDLNFQMLTLKTKKGVRVLPLS
                     QILIPFLEQQLVGKEAYLFEHRGIPFSRQWFFNHLKTFVRSIGYEGLTAQKLREQFIL
                     KEKLAGKSIIELSDILGLKSPVTLEKYYKS"
     gene            43051..43755
                     /locus_tag="VC122_000201"
     CDS             43051..43755
                     /locus_tag="VC122_000201"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014407326.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="segregation/condensation protein A"
                     /protein_id="HEP5640159.1"
                     /translation="MDIKLKDFEGPLDLLLHLVSQYKVDIYEVPIVEVIEQYLNYIET
                     LQVMKLEVAGDYMLMASQLMLIKSRRLLPKVVEHIEEEDLEQDLLEKIEEYSRFKAVS
                     QALAKQHDQRAKWYSKPKQELIFEDAILQEDKTVMDLFLAFSNIMAAKRAVLKTNHTV
                     IERDDYKIEDMMASIKQRLEKENVISLSAIFEECQTLNEVISIFLASLELIKLHVVFV
                     EQLSNFGAIILRKEKK"
     gene            43752..44303
                     /locus_tag="VC122_000202"
     CDS             43752..44303
                     /locus_tag="VC122_000202"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002985894.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="segregation/condensation protein B"
                     /protein_id="HEP5640160.1"
                     /translation="MTYLSQIEALLFVAGEEGLSLRHLASMLSLTPTALQQQLEKLSQ
                     KYEKDQHSSLCLIETANTYRLVTKEGFAELLRAYAKTPMNQSLSRASLEVLSIVAYKQ
                     PITRIEIDDIRGVNSSGALSKLLAFDLIREAGKKDVVGRPHLYATTDYFLDYMGINHL
                     DELIEVSAVEPADEEIALFRTQD"
     gene            44425..45147
                     /locus_tag="VC122_000203"
     CDS             44425..45147
                     /locus_tag="VC122_000203"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003046815.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rRNA pseudouridine synthase"
                     /protein_id="HEP5640161.1"
                     /translation="MRINKYIAHAGIASRRKAEELIKQGLVTLNGQVITDLATTVKSG
                     DVVEIEGSPIYNEEKVYYLLNKPRGAISSVSDDKGRKTVLDLLPQVKERIYPVGRLDW
                     DTSGVLILTNDGDFTDTMIHPRNEIDKVYLARVKGIATKENLRPLTRGIVIDGKKTKP
                     ARYNIVRVEADKNRSIVELTIHEGRNHQVKKMFESVGLLVDKLSRTRFGTVDLKGLRP
                     GEARRLNKKEISQLHNLANTKK"
     gene            45144..45407
                     /gene="yidD"
                     /locus_tag="VC122_000204"
     CDS             45144..45407
                     /gene="yidD"
                     /locus_tag="VC122_000204"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002985889.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="membrane protein insertion efficiency factor
                     YidD"
                     /protein_id="HEP5640162.1"
                     /translation="MMKKLLIVSVKAYQKYISPLSPPSCRYKPTCSAYMLTAIEKHGT
                     KGILMGIARILRCHPFVAGGVDPVPEDFSLMRNKNTSKNAEKA"
     gene            45585..46133
                     /locus_tag="VC122_000205"
     CDS             45585..46133
                     /locus_tag="VC122_000205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002990965.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (uridine(34)/cytosine(34)/5-
                     carboxymethylaminomethyluridine(34)-2'-O)-
                     methyltransferase TrmL"
                     /protein_id="HEP5640163.1"
                     /translation="MTTKELINKNDKVKKARNHIVLFQPQIPQNTGNIARTCAATNAP
                     LHIIKPMGFPIDDRKMKRAGLDYWDKLELHFYDHLEQFINQCHGQLHLISKFAVNNYS
                     QATYADGDSHYFLFGREDTGLPEDFMREHAEKALRIPMNDEHVRSLNVSNTVCMVIYE
                     ALRQQGFQGLELKHTYEHDKLK"
     regulatory      46242..46366
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00050"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="FMN riboswitch; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="flavin mononucleotide"
                     /db_xref="RFAM:RF00050"
     gene            46445..47008
                     /locus_tag="VC122_000206"
     CDS             46445..47008
                     /locus_tag="VC122_000206"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011889125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ECF transporter S component"
                     /protein_id="HEP5640164.1"
                     /translation="MSKTHKMIMIGILSAISFLLMLVSFAIIPGAAFLKIEFSIIPVL
                     FGLMIMDLKSAYLILLLRSLLKLFLNNRGVNDFIGLPMNIIAIALFVTAFALVWNRQK
                     TLSQYVFASLLGTGLLTFGMVVLNYTFAIPLYAIFANIDIRAYIGVTKYMMTMVIPFN
                     LVEGLIFAITFYFVYIASKPILERYLH"
     gene            47010..47663
                     /locus_tag="VC122_000207"
     CDS             47010..47663
                     /locus_tag="VC122_000207"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002990960.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatase PAP2 family protein"
                     /protein_id="HEP5640165.1"
                     /translation="MMTNKQTHFLIASFALLIFVIIGYTVKFFPERLALLDNTIQAEI
                     RGNLPIVLTQFFRGVTVFGNVMTQVLLVIVSVLVLFFMKWKIEALFILSNGAIAAFLI
                     TTLKLFYQRPRPAIEHLVYAGGYSFPSGHAMGSMLIFGSLLIICYQRLHSKLLQFVTS
                     MIFIILILLIGLSRIYLGVHYPSDILAGFVLGFGILHFIYPFYKQKRFEWRFLLKQD"
     gene            47956..48876
                     /locus_tag="VC122_000208"
     CDS             47956..48876
                     /locus_tag="VC122_000208"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014407327.1"
                     /note="This family includes YhcC from E. coli K-12, an
                     uncharacterized radical SAM protein; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR01212 family radical SAM protein"
                     /protein_id="HEP5640166.1"
                     /translation="MKKRYQTLNEHYRQLFGAKMFKVPIDAGFDCPNRDGTVAHGGCT
                     FCTVSGSGDAIVAPDAPIKEQFYKEIDFMHRKWPDVNRYLVYFQNFTNTHDTVDVIRD
                     RYEQAINEPGVVGINIGTRPDCLPDDTIAYLAELSERMHVTVELGLQTTYEETSRLIN
                     RAHSYDLYKETVRRLRHYPNINIVSHLINGLPKETHDMMLENVRRCVTDNDIQGIKLH
                     LLHLMTNTRMQRDYHEGRLKLLSQKDYVSIICDQLEIIPKHIVIHRITGDAPRDMLIG
                     PMWSLNKWEVLNAIDKEMERRGSFQGCKVD"
     gene            48915..49469
                     /locus_tag="VC122_000209"
     CDS             48915..49469
                     /locus_tag="VC122_000209"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002990955.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HEP5640167.1"
                     /translation="MLKRPIHLSHDFLAEVVDKSSVVVDATMGNGNDTAFLAQLAKKV
                     YAFDVQEQAIRKTSERLAQLGLSNAELILAGHEAVDQYVTEPVRAAIFNLGYLPSADK
                     SIITLPNTTLQALSKLLTLLMVGGRIAIMVYYGHDGGSLEKDALLDFVKQLDQRKVSA
                     MLYQPLNQVNTPPFLIMLEKLADV"
     gene            49602..50936
                     /locus_tag="VC122_000210"
     CDS             49602..50936
                     /locus_tag="VC122_000210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002990953.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HlyC/CorC family transporter"
                     /protein_id="HEP5640168.1"
                     /translation="MEDPVSQPLVIQFLLLVVLTLLNAFFSASEMALVSLNRSRVEQK
                     AADGDKKYARLLRVLEEPNHFLSTIQVGITFISLLSGASLSASLGKVISGWLGNSATA
                     RTAGTIISLVFLTYVSIVLGELYPKRIAMNLKDKLAIVSAPIIIGLGRLVSPFVWLLS
                     ASTNLLSRLTPMTFDDADEQMTRDEIEYMLSKSEATLDAEEIEMLQGVFSLDEMMARE
                     VMVPRTDAFMIDINDDPLENIQEILKQSFSRIPVYDVDKDKIIGLIHTKRLLESGFRQ
                     GFDQINMRKMLQEPLFVPETIFVDDLLRQLRNTQNQMAILLDEYGGVAGLVTLEDLLE
                     EIVGEIDDETDKAEQFVHEIGDNTYIVVGTMTLNEFNDYFDTELESDDVDTIAGFYLT
                     GIGTIPSQEQKEAYEIDNKDKHLVLINDKVKDGRITKLKLILSNIEQIIEED"
     gene            51014..51805
                     /gene="pflA"
                     /locus_tag="VC122_000211"
     CDS             51014..51805
                     /gene="pflA"
                     /locus_tag="VC122_000211"
                     /EC_number="1.97.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011889124.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate formate lyase-activating protein"
                     /protein_id="HEP5640169.1"
                     /translation="MTEKDYGQVTGMVHSTESFGSVDGPGIRFIIFLQGCKLRCQYCH
                     NPDTWEMETNNSKIRTVNDVLKEALQYKHFWGKKGGITVSGGEAMLQIDFITALFIEA
                     KKLGIHTTLDTCGFTYRPTPEYHQVLDNLLAVTDLILLDLKEIDEKQHKIVTRQPNKN
                     ILQFARYLSDKQIPVWIRHVLVPGLTDIDDHLTRLGEFVKTLKNVDKFEVLPYHTMGE
                     FKWRELGIPYQLEGVKPPTKERVQNAKNLMQTESYTEYMNRIHQS"
     gene            51936..52871
                     /locus_tag="VC122_000212"
     CDS             51936..52871
                     /locus_tag="VC122_000212"
                     /EC_number="3.6.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000036024.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="manganese-dependent inorganic pyrophosphatase"
                     /protein_id="HEP5640170.1"
                     /translation="MSKILVFGHQNPDTDAIASSYAFDYLSQKAFGLDTEVVALGTPN
                     EETAFALDYFGVEAPRVVESAKAQGSEQVILTDHNEFQQSIADIREVEVYGVVDHHRV
                     ANFETANPLYMRVEPVGSASSIVYRMFKENGIEVPKAIAGMLLSGLISDTLLLKSPTT
                     HVSDHLVAEELAELAEVNLEDYGMALLKAGTNLASKSEVELIGIDAKTFELNGNAVRV
                     AQVNTVDIAEVLERQEAIEAAIKDAMAAEGYSDFVLMITDIVNSNSEILAIGANMDKV
                     EAAFNFTLDNNHAFLAGAVSRKKQVVPQLTESFGA"
     gene            52947..53600
                     /locus_tag="VC122_000213"
     CDS             52947..53600
                     /locus_tag="VC122_000213"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009880862.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1803 domain-containing protein"
                     /protein_id="HEP5640171.1"
                     /translation="MITVFHSDKLTRQPFFQDLINYLDQHDHVILREIKKAFPNVTGI
                     DKAIESYVQAGYIRRENKRYGINLPLVSSDQQLALDTMLFVDTCSAMYENILAVVFET
                     QLTNQTNHVMIKEKTNITRDDLTLANYFYRLKRGEKPSAEQLDLYDLLGDVNQEYALK
                     YMTTFLLKFTRKDLVMQKRPDIFVEALVTLGYLKQVEPTTYQLLMTLDKESLTFIAP"
     gene            complement(53645..54682)
                     /locus_tag="VC122_000214"
     CDS             complement(53645..54682)
                     /locus_tag="VC122_000214"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011284529.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron ABC transporter permease"
                     /protein_id="HEP5640172.1"
                     /translation="MLFMVNKKRRPLLKSHRHLLGLYTSLILLLVSLMGLALSLGESH
                     LSFLDLIHVFLGKSSHAISFIVINIRLPRILAACLGGGSLALSGLLLQRLTRNPLADS
                     GVLGITIGAGISLAIVVSFSFFEQAHISHYLPLFAMLGAVVTTFSVYWLSLTKQGQID
                     PTRLILTGVAVTTMLSSLMVALVGHINRYKVDLVINWLSGQLIGDDWPTLSVIAPLLL
                     CFWLLTYSQAHFLNIMGLADNTAIGLGLPLNRKRRLILVLAAGLGALSVLLVGNISFI
                     GLIAGHFSTYLVGSNHKITIPISILIGMILLLVADTVGRVYLVGTNIQTGILVSLIGA
                     PYFLYLMAKTK"
     gene            complement(54643..55695)
                     /locus_tag="VC122_000215"
     CDS             complement(54643..55695)
                     /locus_tag="VC122_000215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002985865.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron ABC transporter permease"
                     /protein_id="HEP5640173.1"
                     /translation="MKTKHPFLCLERFCLKKTQIITKSHIFWLVFVLLSFSLCVAIYC
                     HLRFGAVALSHQDLNSILFGKQNGHKANVLLAIRLPRLFGATLTGSALAVSGTIMQAI
                     TRNPIAEPGLLGINAGAGLALVLAYAFVPHLHYSLIILLSLLGSSLAATLVFGLSYQS
                     GKGYHQLRLVLAGAMVSILLSALGQGITNYYHLANAVIGWQAGGLVGVNWQMIGYIAP
                     LIILSLCLAQLLSYHLTVLSLSESQAKALGQKTNLISAVFMILVLILSSAAVAIAGSI
                     SFIGLVIPHLMKHFTPHHYRYLLPLCAVSGASFMVWVDIACRNLNPPYETPLGALVSL
                     IGFPCFLWLIRRGGRY"
     gene            complement(55685..56617)
                     /locus_tag="VC122_000216"
     CDS             complement(55685..56617)
                     /locus_tag="VC122_000216"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010921927.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-hydroxamate ABC transporter
                     substrate-binding protein"
                     /protein_id="HEP5640174.1"
                     /translation="MKKLTLLLTLCLTTITLIACGNQATNHSNTASKSLSPMPQIAGV
                     TYYGDIPKQPKRVVSLASTYTGYLKKLDMNLVGVTSYDKKNPILAKTVKKAKQVAATD
                     LEAITTLKPDLIVVGSTEENIKQLAEIAPVISIEYRKRDYLQVLSDFGRIFNKEGKAK
                     KWLKDWKTKTAAYEKEVKAVTGDKATFTIMGLYEKDVYLFGKDWGRGGEVIHQAFHYD
                     APEKVKTEVFKQGYLSLSQEVLPDYIGDYVVVAAEDDKTGSALYESKLWQSIPAVKKH
                     HVIKVNANVFYFTDPLSLEYQLETLREAILSSEN"
     gene            complement(56643..57425)
                     /locus_tag="VC122_000217"
     CDS             complement(56643..57425)
                     /locus_tag="VC122_000217"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002985861.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEP5640175.1"
                     /translation="MTTISAEDLTIAYEQRTIIDKLSFYIPEGKITTIIGANGCGKSS
                     LLKALTRLLPPKQGVVYLNGQNIATLETKEVAKKLALLPQVQEATNGITVYELVSYGR
                     FPHQSYFGNLSPADKKAIHWAMQATNVMAYADQPVDALSGGQRQRVWLAMALAQGTDT
                     IFLDEPTTYLDLNHQLEILELVKSLNKDAGKTIVMVLHDLNLSARYSDHLIAMKHGKI
                     HYTGTIADVMTSPIIQDIFQIKPVLVDDPIHNCPIVLTYQLQ"
     gene            complement(57671..59116)
                     /locus_tag="VC122_000218"
     CDS             complement(57671..59116)
                     /locus_tag="VC122_000218"
                     /EC_number="6.3.2.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012560471.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramoyl-L-alanyl-D-glutamate--L-
                     lysine ligase"
                     /protein_id="HEP5640176.1"
                     /translation="MITIEQLLDILKKDHNFREVLDADGYHYHYQGFSFERLSYDSRQ
                     VDGKTLFFAKGATFKADYLKEAITNGLQLYISEVDYELGIPVVLVTDIKKAMSLIAMV
                     FYGNPQEKLKLLAFTGTKGKTTAAYFAYHMLKESYKPAMFSTMNTTLDGKTFFKSQLT
                     TPESLDLFAMMAECVTNGMTHLIMEVSSQAYLVDRVYGLTFDVGVFLNISPDHIGPIE
                     HPTFEDYFYHKRLLMENSRAVVINSGMDHFSFLADQVADQEHVFYGPLSDNQITTSQA
                     FSFEAKGQLAGHYDIQLIGHFNQENAMAAGLACLRLGASLADIQKGIAKTRVPGRMEV
                     LTMTNHAKVFVDYAHNGDSLEKLLSVVEEHQTGKLMLILGAPGNKGESRRADFGRVIH
                     QHPNLTVILTADDPNFEDPEDISKEIASHIARPVEIISDREQAIQKAMSLCQGAKDAV
                     IIAGKGADAYQIVKGQQVAYAGDLAIARHYL"
     gene            59204..60838
                     /locus_tag="VC122_000219"
     CDS             59204..60838
                     /locus_tag="VC122_000219"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015016717.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide biosynthesis protein"
                     /protein_id="HEP5640177.1"
                     /translation="MSTEKKQLTQEELMVQGAAWSTAGNFISRLLGVLYIIPWYIWMG
                     QYAIQANALFNMGYNVYAYFLLISTTGLNVAIAKQVAKYNSMGQTEHSYQLIRSTLKL
                     MLGLGLIFSAIMYLGSPLFASLSGGDDTLVPIMHSLSLAVFIFPVMSVIRGIFQGHNN
                     IKPYAISQIAEQLIRVIWMLLTTFLIMKLGSGDYASAVTQSTFAAFIGMVASMGVLGY
                     YLWKQGLLAAIFSKPDHTVSIDIKGLLLETLKESIPFIVTGSAIQAFQLIDQWTFVNT
                     MTLFTDYSRSQLLVLFGYFNANPAKITMVLIAVAASIGGVGIALLTENYVKKDMKAAA
                     RLIINNIEMLVMFLLPALTGAIILARPLYSVFYGASEDRAIHLFVAVLFQTLLMALYT
                     LFSPMLQALFENRKAIYYFAYGILIKLVLQIPLIYLLHAYGPLLATTIALVVPIYLMY
                     RRLYQVTHFNRKLLQKRLLLTLIETLLMGLVVFVANWLLGYVFKPTGRLTSLLYLLII
                     GGLGMTVYTALTLLTHQLDKLIGSKASRLRQKLGWH"
     gene            61006..61635
                     /gene="upp"
                     /locus_tag="VC122_000220"
     CDS             61006..61635
                     /gene="upp"
                     /locus_tag="VC122_000220"
                     /EC_number="2.4.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002883819.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="uracil phosphoribosyltransferase"
                     /protein_id="HEP5640178.1"
                     /translation="MGKCQVISHPLIQHKLSILRRQTTSTKDFRELVNEIAMLMGYEV
                     SRDLPLEDVDIQTPVSKTVQKQLAGKKLAIVPILRAGIGMVDGLLSLVPAAKVGHIGM
                     YRNEETLEPVEYLVKLPEDINQRQIFLVDPMLATGGSAILAVDSLKKRGAANIKFVCL
                     VAAPEGVKKLQEAHPDIDIFTAALDDHLNEHGYIVPGLGDAGDRLFGTK"
     gene            61859..62449
                     /locus_tag="VC122_000221"
     CDS             61859..62449
                     /locus_tag="VC122_000221"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002274529.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent Clp protease proteolytic subunit"
                     /protein_id="HEP5640179.1"
                     /translation="MIPVVIEQTSRGERSYDIYSRLLKDRIIMLTGPVEDNMANSVIA
                     QLLFLDAQDNTKDIYLYVNTPGGSVSAGLAIVDTMNFIKADVQTIVMGMAASMGTVIA
                     SSGTKGKRFMLPNAEYMIHQPMGGTGGGTQQTDMAIAAEHLLKTRHRLEKILAQNAGK
                     TIKQIHKDAERDYWMSAEETLTYGFIDEIMENNELK"
     gene            62944..63219
                     /locus_tag="VC122_000222"
     CDS             62944..63219
                     /locus_tag="VC122_000222"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002985847.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YlbG family protein"
                     /protein_id="HEP5640180.1"
                     /translation="MFQKQERIGLVVYLYYNRDARKLSKFGDLYYHSKRSRYLIVYIN
                     KNDLDTKLEEMRRLKCVKDIRPSAFDDIDRQFVGNLHRDETNNHQKG"
     gene            63468..64103
                     /locus_tag="VC122_000223"
     CDS             63468..64103
                     /locus_tag="VC122_000223"
                     /EC_number="2.7.4.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014635306.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dTMP kinase"
                     /protein_id="HEP5640181.1"
                     /translation="MITGKLITVEGPDGAGKTTVLEQLIPLLKQKVAQEILTTREPGG
                     VAISEYIRELILDINHTTMDPKTELLLYIAARRQHLVEKVLPALEAGQLVFIDRFIDS
                     SVAYQGAGRGLIKADIQWLNEFATDGLEPDLTLYFDVPSEIGLARINANQQREVNRLD
                     LETIEIHQRVRKGYLALAKEHPKRIVTIDATKPLKEVVSVALEHVLALLLA"
     gene            64121..64996
                     /locus_tag="VC122_000224"
     CDS             64121..64996
                     /locus_tag="VC122_000224"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009880726.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit delta'"
                     /protein_id="HEP5640182.1"
                     /translation="MDLAQKAPNVYQAFQTILKKDRLNHAYLFSGDFANEEMALFLAK
                     VIFCEQKKDQTPCGHCRSCQLIEQGDFADVTVLEPTGQVIKTDVVKEMMANFSQTGYE
                     NKRQVFIIKDCDKMHVNAANSLLKYIEEPQGEAYIFLLTNDDNKVLPTIKSRTQVFQF
                     PKNEAYLYQLAQEKGLLNHQAKLVAKLATNTSHLERLLQTSKLLELITQAERFVSIWL
                     KDQLQAYLALNRLVQLATEKEEQDLVLTLLTLLLARERAQTPLTQLEAVYQARLMWQS
                     NVNFQNTLEYMVMSE"
     gene            65499..65822
                     /gene="yabA"
                     /locus_tag="VC122_000225"
     CDS             65499..65822
                     /gene="yabA"
                     /locus_tag="VC122_000225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002985838.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA replication initiation control protein YabA"
                     /protein_id="HEP5640183.1"
                     /translation="MNKKELFDAFDGFSQNLMVTLAEIEAMKKQVQSLVEENTILRLE
                     NTKLRERLSHLEHETVAKNPSKQRKDHLEGIYDEGFHICNFFYGQRRENDEECMFCRE
                     LLDRK"
     gene            65827..66690
                     /gene="rsmI"
                     /locus_tag="VC122_000226"
     CDS             65827..66690
                     /gene="rsmI"
                     /locus_tag="VC122_000226"
                     /EC_number="2.1.1.198"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003046907.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA
                     (cytidine(1402)-2'-O)-methyltransferase"
                     /protein_id="HEP5640184.1"
                     /translation="MQVQKSFKDKKTSGTLYLVPTPIGNLQDMTFRAVATLKEVDFIC
                     AEDTRNTGLLLKHFDIATKQISFHEHNAYEKIPDLIDLLISGRSLAQVSDAGMPSISD
                     PGHDLVKAAIDSDIAVVALPGASAGITALIASGLAPQPHVFYGFLPRKAGQQKAFFED
                     KHHYPETQMFYESPYRIKDTLTNMLACYGDRQVVLVRELTKLFEEYQRGSISEILSYL
                     EETPLKGECLLIVAGAQVDSEVELTADVDLVSLVQKEIQAGAKPNQAIKTIAKAYQVN
                     RQELYQQFHDL"
     gene            66717..67109
                     /locus_tag="VC122_000227"
     CDS             66717..67109
                     /locus_tag="VC122_000227"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003058684.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEP5640185.1"
                     /translation="MTEDQATILILGVYGIILAVFLLSALLCLIANWRLFKRAGYKGW
                     YSLIPFYAGFVKHKITFGEDNKWFYFIGWVIAIYYYYVHFCYVRAFGGSKGLAVFGLF
                     FPGIASLIVAFGKTYTTQECHPVPHLLN"
     gene            complement(67156..67785)
                     /locus_tag="VC122_000228"
     CDS             complement(67156..67785)
                     /locus_tag="VC122_000228"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003049817.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="copper homeostasis protein CutC"
                     /protein_id="HEP5640186.1"
                     /translation="MIKEFCAENLTLLPTLDAGQVSRVELCDNLAVGGTTPSYGVIKE
                     ACQLLHDKKISVATMIRPRGGDFVYNDLELKAMEEDILKAVEAGSDALVLGLLTSENQ
                     LDTDAIEQLLPATQGLPLVFHMAFDRIPTDHQHQALDQLIDYGFVRVLTHGSPEATPI
                     TDNVEQLKSLVTYANKRIEIMIGGGVTAENCQSLSQLTGTAIVHGTKII"
     gene            68084..68440
                     /locus_tag="VC122_000229"
     CDS             68084..68440
                     /locus_tag="VC122_000229"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011184219.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arsenate reductase family protein"
                     /protein_id="HEP5640187.1"
                     /translation="MITIYQYPKCSTCQRAMAELKQLVSDFEAIDIKANPPKAQDLKH
                     WMETSGYTIKNFFNTSGNSYRELGLKDKIDQLSLDKAAELLATDGMLIKRPILIKDGN
                     VLQVGYRKPYQELDLG"
     gene            complement(68514..69341)
                     /gene="xth"
                     /locus_tag="VC122_000230"
     CDS             complement(68514..69341)
                     /gene="xth"
                     /locus_tag="VC122_000230"
                     /EC_number="3.1.11.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014635309.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease III"
                     /protein_id="HEP5640188.1"
                     /translation="MKLISWNIDSLNAALTGESPRALLSRAVLDTLVAQDADIIAIQE
                     TKLSAKGPTKKHIETLLSYFPNYLHVWRSSVEPARKGYAGTMFLYKNTLNPVITFPEI
                     GAPTTMDAEGRIITLEFEDFFVTQVYTPNAGDGLRRLDDRQIWDHKYADYLTELDAQK
                     PVLATGDYNVAHKEIDLANPSSNRRSPGFTDEERQGFTNLLARGFTDTFRHLHGDIPH
                     VYTWWAQRSKTSKINNTGWRIDYWLASNRIVDKVKRSEMISSGERQDHTPILLDIDL"
     gene            69575..70756
                     /gene="lctO"
                     /locus_tag="VC122_000231"
     CDS             69575..70756
                     /gene="lctO"
                     /locus_tag="VC122_000231"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011054225.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-lactate oxidase"
                     /protein_id="HEP5640189.1"
                     /translation="MAQKTVITEETTDFVMDFKTSSAEGNVDFINVFDLEKMAQQVIP
                     KGAFGYIASGAGDTFTLHENIRSFNHKLIVPHSLKGVENPSTEITFDGDHLTSPLILA
                     PVAAHKLANEQGEVASAKGLKEFGSIYTTSSYSTTDLPEISAALGGTPHWFQFYCSKD
                     DGINRNIMDRVKAQGCKAIVLTADATVGGNREVDRRNGFVFPVGMPIVQEYLPDGAGK
                     TMDYVYKSAKQALTSKDIEYIATYSGLPVYVKGPQCAEDTLRALDAGASGIWVTNHGG
                     RQLDGGPAAFDSLQEVAEAVDHKVPIVFDSGIRRGQHIFKALASGADLVALGRPAIYG
                     LAMGGSIGTRQVFEKLNDELKMVMQLAGTQTIQDVKAFNLRHNPYDSSIPFDQNALRL
                     D"
     gene            71022..75959
                     /gene="scpC"
                     /locus_tag="VC122_000232"
     CDS             71022..75959
                     /gene="scpC"
                     /locus_tag="VC122_000232"
                     /EC_number="3.4.21.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010921938.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CXC chemokine-degrading serine protease SpyCEP"
                     /protein_id="HEP5640190.1"
                     /translation="MEKKQRFSLRKYKSGTFSVLIGSVFLMMTTTVAADELTTTSEPT
                     ITNHAQQQAQHLTNTELSSAESQSPDTSQITPKINREKEQPQGLVSEPTTTELADTDA
                     APMANTGPDATQKSASLPPVNTDVHDWVKTKGAWDKGYKGQGKVVAVIDTGIDPAHQS
                     MRISDVSTAKVKSKEDMLARQKAAGINYGSWINDKVVFAHNYVENSDNIKENQFEDFD
                     EDWENFEFDAEPKAIKKHKIYRPQSTQAPKETVIKTEETDGSHDIDWTQTDDDTKYES
                     HGMHVTGIVAGNSKEAAATGERFLGIAPETQVMFMRVFANDVMGSAESLFIKAIEDAV
                     ALGADVINLSLGTANGAQLSGSKPLMEAIEKAKKAGVSVVVAAGNERVYGSDHDDPLA
                     TNPDYGLVGSPSTGRTPTSVAAINSKWVIQRLMTVKELENRADLNHGKAIYSESVDFK
                     DIKDSLGYDKSHQFAYVKESTDAGYNAQDVKGKIALIERDPNKTYDEMIALAKKHGAL
                     GVLIFNNKPGQSNRSMRLTANGMGIPSAFISHEFGKAMSQLNGNGTGSLEFDSVVSKA
                     PSQKGNEMNHFSNWGLTSDGYLKPDITAPGGDIYSTYNDNHYGSQTGTSMASPQIAGA
                     SLLVKQYLEKTQPNLPKEKIADIVKNLLMSNAQIHVNPETKTTTSPRQQGAGLLNIDG
                     AVTSGLYVTGKDNYGSISLGNITDTMTFDVTVHNLSNKAKTLRYDTELLTDHVDPQKG
                     RFTLTSRSLKTYQGGEVTVPANGKVTVRVTMDVSQFTKELTKQMPNGYYLEGFVRFRD
                     SQDDQLNRVNIPFVGFKGQFENLAVAEESIYRLKSQGKTGFYFDESGPKDDIYVGKHF
                     TGLVTLGSETNVSTKTISDNGLHTLGTFKNADGKFILEKNAQGNPVLAISPNGDNNQD
                     FAAFKGVFLRKYQGLKASVYHASDKEHKNPLWVSPESFKGDKNFNSDIRFAKSTTLLG
                     TAFSGKSLTGAELPDGYYHYVVSYYPDVVGAKRQEMTFDMILDRQKPVLSQATFDPET
                     NRFKPEPLKDRGLAGVRKDSVFYLERKDNKPYTVTINDSYKYVSVEDNKTFVERQADG
                     SFILPLDKAKLGDFYYMVEDFAGNVAIAKLGDHLPQTLGKTPIKLKLTDGNYQTKETL
                     KDNLEMTQSDTGLVTNQAQLAVVHRNQPQSQLTKMNQDFFISPNEDGNKDFVAFKGLK
                     NNVYNDLTVNVYAKDDHQKQTPIWSSQAGASASAIESTAWYGITARGSKVMPGDYQYV
                     VTYRDEHGKEHQKQYTISVNDKKPMITQGRFDTINGVDHFTPDKTKALGSSGIVREEV
                     FYLAKKNGRKFDVTEGKDGITVSDNKVYIPKNPDGSYTISKRDGVTLSDYYYLVEDRA
                     GNVSFATLRDLKAVGKDKAVVNFGLDLPVPEDKQIVNFTYLVRDADGKPIENLEYYNN
                     SGNSLILPYGKYTVELLTYDTNAAKLESDKIVSFTLSADNNFQQVTFKMTMLATSQIT
                     AHFDHLLPEGSRVSLKTAQGQLIPLEQSLYVPKAYGKTVQEGTYEVVVSLPKGYRIEG
                     NTKVNTLPNEVHELSLRLVKVGDASDSTGDHKVMSKNNSQALTASATPTKTTTSATAK
                     ALPSAGEKMGLKLRIVGLVLLGLTCVFSRKKSTKD"
     gene            76732..77439
                     /locus_tag="VC122_000233"
     CDS             76732..77439
                     /locus_tag="VC122_000233"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002990882.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1275 domain-containing protein"
                     /protein_id="HEP5640191.1"
                     /translation="MSKKKRKHYQVYDGLRCAMTLCFISGYVNAFTYMTQGKRFAGVQ
                     TGNLLSFAIRLSEQQLKEALQFLLPMIVFMLGQSFTYFMHRWATKKGLHWYLLSSVIL
                     TGIAFGMALFTPFLPSNVTVAALAFFASIQVDTFKTLRGASYANVMMTGNIKNAAYLL
                     TKGLYEKNHELTHIGRNTLIVILAFAVGVVCSTLLCIAYGEYALMPILMPLLYVNYLL
                     AQEFYHIQTKIKPIKRT"
     gene            77679..79679
                     /gene="metG"
                     /locus_tag="VC122_000234"
     CDS             77679..79679
                     /gene="metG"
                     /locus_tag="VC122_000234"
                     /EC_number="6.1.1.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002985818.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methionine--tRNA ligase"
                     /protein_id="HEP5640192.1"
                     /translation="MMKKPFYITTPIYYPSGKLHIGSAYTTIACDVLARYKRLMGHEV
                     FYLTGLDEHGQKIQTKAKEAGITPQTYVDNMAKDVKALWQLLDISYDTFIRTTDDYHE
                     EVVAAVFEKLLAQDDIYLGEYSGWYSVSDEEFFTESQLEEVFRDEDGKVIGGIAPSGH
                     QVEWVSEESYFLRLSKYADRLVAFFKERPDFIQPDGRMNEMVKNFIEPGLEDLAVSRT
                     TFTWGVPVPSDPKHVVYVWIDALLNYATALGYGQANHANFDKFWNGTVFHMVGKDILR
                     FHSIYWPILLMMLDLPMPDRLIAHGWFVMKDGKMSKSKGNVVYPEMLVERFGLDPLRY
                     YLMRSLPVGSDGTFTPEDYVGRINYELANDLGNLLNRTVAMINKYFDGTVPAYVDNGT
                     AFDADFSQLIDAQLADYHKHMEAVDYPRALEAIWTIIARTNKYIDETAPWVLAKEDGD
                     KAQLASVMAHLAASLRVVAHVIQPFMMETSAAIMAQLGLAPVSDLSTLALADFPANTK
                     VVAKGTPIFPRLDMEAEIDYIKAQMGDSSAISQEKEWVPEEVALKSEKDVITFETFDA
                     VEIRVAEVKEVSKVEGSEKLLRFRVDAGDGQDRQILSGIAKCYPNEQELVGKKLQIVA
                     NLKPRKMMKQYISQGMILSAEHGDQLTVLTVDSSVPNGSIIG"
     gene            80170..81183
                     /gene="nrdF"
                     /locus_tag="VC122_000235"
     CDS             80170..81183
                     /gene="nrdF"
                     /locus_tag="VC122_000235"
                     /EC_number="1.17.4.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002990873.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class 1b ribonucleoside-diphosphate reductase
                     subunit beta"
                     /protein_id="HEP5640193.1"
                     /translation="MTQHYYERSQSPIEYALSETQKQLRSINWNYLNDDKDLEVWNRV
                     TQNFWLPEKVPVSNDLNSWRSLGEDWQQLITRTYTGLTLLDTVQATVGDVAQIQHSQT
                     DHEQVIYTNFAFMVGIHARSYGTIFSTLCSSEQIEEAHEWVVSTQSLQDRSRVLIPYY
                     TGDDPLKSKVAAAMMPGFLLYGGFYLPFYLSARGKMPNTSDIIRLILRDKVIHNYYSG
                     YKYQQKVARLSPEKQAEMKAFVFDLLYELIDLEKAYLRELYAGFDLAEDAIRFSLYNA
                     GKFLQNLGYDSPFTDEETRISPEVFAQLSARADENHDFFSGNGSSYVMGITEETTDDD
                     WEF"
     gene            81187..81675
                     /gene="nrdI"
                     /locus_tag="VC122_000236"
     CDS             81187..81675
                     /gene="nrdI"
                     /locus_tag="VC122_000236"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002985812.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class Ib ribonucleoside-diphosphate reductase
                     assembly flavoprotein NrdI"
                     /protein_id="HEP5640194.1"
                     /translation="MAELIIVYFSSKSNNTHRFVQKLGLPAQRIPVDNRPLEVSTHYL
                     LIVPTYAAGGSDAKGAVPKQVIRFLNNPNNRKHCKGVISSGNTNFGDTFALAGPIISQ
                     KLQVPLLHQFELLGTATDVKKVQAIFARLKHHTHDKQKQINNLITERTHPCHKPMRHT
                     SH"
     gene            81642..83822
                     /gene="nrdE"
                     /locus_tag="VC122_000237"
     CDS             81642..83822
                     /gene="nrdE"
                     /locus_tag="VC122_000237"
                     /EC_number="1.17.4.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011017432.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class 1b ribonucleoside-diphosphate reductase
                     subunit alpha"
                     /protein_id="HEP5640195.1"
                     /translation="MSQTNASYLSLNALTRFKKPDGSYHFDSDKEAVRRYLEEHVSPN
                     QMAFNSLEDKLAYLINEGYYEQAIFDAYPNDLIKEAFHYAYQQGYRFLNLMGAMKFYQ
                     SYALKTLDGKQYLETFEDRAVMNALFLADGDQTFVFDVIDAILHRRFQPATPTFLNAG
                     KKRRGEYISCYLLRVEDNMESISRAISTSLQLSKRGGGVALCLTNLREIGAPIKGIEN
                     QATGIVPVMKLLEDSFSYANQLGQRQGAGAVYLHAHHPEVLTFLDTKRENADEKIRIK
                     SLALGLVIPDITFQLAKENKDMALFSPYDIKRAYGKDMSDISITEEYDKLLANPAIKK
                     TYISARKFFQLIAELHFESGYPYLLFDDTVNKRNPHAKKGRIVMSNLCSEIAQVSTPS
                     TFKEDLSFETIGEDICCNLGSINIAQAMADAPHFEQLITTSIRALDRVSRVSDLNCAP
                     SVETGNAANHAVGLGAMNLHGFLATNHIYYDTKEAVDFTDLFFHAMAYYAFKASCQLA
                     KEKGAFAGFSLSTYADGTYFAKYLQEDAKPQTAKVATLLQDYGFTLPTVADWQALVAD
                     IKQFGLANAHLLAVAPTGSISYLSSCTPSLQPVVAPVEVRKEGSLGRIYVPAYQIDQA
                     NYAYYERGAYEVGPKAIIDVVAAAQKHVDQAISLTLFMTDQATTRDLNRSYIQAFKQN
                     CASIYYVRVRQDVLAGSEQYDEDSLVTAPGASDETTTECQSCMI"
     gene            complement(84027..84779)
                     /locus_tag="VC122_000238"
     CDS             complement(84027..84779)
                     /locus_tag="VC122_000238"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002985808.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ADP-ribosyltransferase"
                     /protein_id="HEP5640196.1"
                     /translation="MLKKRYQLAMILLLSCFSLVWQTEGLVELFVCEHYERAVCEGTP
                     AYFTFSDQKGAETLIKKRWGKGLVYPRAEQEAMAAYTCQQAAPINTSLDKTKGELSQL
                     TPELRDQVAQLDAATHRLVIPWNIVVYRYVYETFLRDIGVSHADLTSYYRNHQFDPHI
                     LCKIKLGTRYTKHSFMSTTALKNGAMTHRPVEVRICVKKGAKAAFVEPYSAVPSEVEL
                     LFPRGCQLEVVGAYVSQDQKKLHIEAYFKGSL"
BASE COUNT        27145 a        15400 c        17533 g        25127 t
ORIGIN      
        1 gtaatcattt tttgatttac agttattatc atgtgttatc tggcgactgt cctgtttaca
       61 agtaagttca caagtatggg ataaaatagt ataaactata gtaaatactg aatagtcata
      121 gaaaaatatc ggcataggtc agaaaagctg atatgtttaa tactttttgc ggatatctac
      181 tgtatttaaa tgtatttgat atccttgtca aggatatacg tctaaacaag ttcaaaacga
      241 aatcatttgt gattatctta tcaggagttg actcttgata agatttttgt atatcagtac
      301 tgtggtacgt ttagatagct tggcagtgaa tttatagagt gtcaaacaac gagtgttttg
      361 tgttacacta accattagaa gagtctattg ttgagcatat caaggtgtta tcttttcgag
      421 ttgaggtatc atatctagta tatttaacta tggttttaaa tgctatttca aaaaaaatat
      481 gataaaatag aaagtactaa ttattttgga gatgacatga gtatcgatga ttataggccg
      541 acctatatgg tagaggctat ttatgattta agagcaaatg atttgttgcg tcatggtatt
      601 acggctgttt tagtggattt agataatacc ttaattgcat ggaataatcc tgatggcaca
      661 ccagaggttc gcgcttggct tgatgaaatg actatcgcgg atattagcgt ggttgttgtt
      721 tcaaataata aacattctcg tgttgagcgt gccgtttcac gctttggtgt tgactttatt
      781 agccgggctt tgaagccatt tgcttatggt attgaaaaag ccattgctcg ttatggattt
      841 gatcgcaacg aggttatcat ggttggagat cagctaatga ctgatatcag agctagtcat
      901 agagctggaa taaaatcagt tttagtcaaa ccacttgttg cttctgatgc ttggaatact
      961 aaaattaacc gctggcgtga acgccgtgtg atggcaaaat tagaagagaa atatggtaaa
     1021 ttatcatatc aaaaaggaat ttaatggaag aattattttg tataggctgt ggtatccaga
     1081 ttcaaactga agacaaagaa aaagctggtt ttacgccagc tgctgctttg aaaaagggta
     1141 tggaaacagg agaactctat tgtcagcgct gctttagatt gaggcattat aatgaaatta
     1201 cagatgtcca tattaccgat gatgaatttt taagacttct tcacgaagtg ggtgatagtg
     1261 atgctcttgt ggttaatgtg attgatattt ttgactttaa tggttctatt attccaggtc
     1321 tatctcgctt tatttctggt aatgatgtct tactggttgg gaataaaaaa gatattttgc
     1381 caaagtctgt taaggatggt aaagttactc aatggttgac ggaacgtgca cacgaagaag
     1441 gtcttcgccc tttagacgtt atgttaacga gcgctcaaaa taaatatgcg attaaagact
     1501 tgattgagcg tatcaatgag ttacgtaatg gtcgagatgt ttatgttgtg ggggtcacta
     1561 atgttggtaa atcgacctta attaatgcca ttattcaaga aattacaggg gataaagatg
     1621 tgattacgac gtcccgcttc cctggaacga cccttgataa aattgaaatc ccactggatg
     1681 acggtagctt catatttgat acgccaggta ttattcatcg tcatcaaatg gctcattatt
     1741 tatcccctaa agagctaaaa atagtgagtc ctaagaagga aatcaagcct aagacgtatc
     1801 aattaaaccc agaacaaact ctctttttag gtggtttgtc tcgctttgac tttatcaatg
     1861 gggagcgtca agggtttaca gctttttttg acaatcaact agaattacat cgtacaaaat
     1921 tagctggagc agatgctttt tatgataagc atgttggaac tttgttaact ccgccaggta
     1981 agaaagagtt gacagcattt cctaaattag ttcgtcatga atttacaatt gatcagaaaa
     2041 tggatattgt tttttctggt ctaggatgga ttcgtgttaa tggtcaaaaa gactcaaaag
     2101 caatagttgc tgcatgggct cctgaaggcg tcgctgtcat tgttcgtaaa gcgattattt
     2161 aaaaataaaa aaagaagttg gaaaaaaatg cttacaagta aacaaagagc tttcttaaaa
     2221 tctgaggctc atagcttaaa accgattgta caaattggca aaaatggatt aaatgaccac
     2281 attaagacta gtattcgtca agctttggat gctagagaat tgataaaggt cactttgttg
     2341 caaaatactg atgaagatat tcatgaagta gcagagattt tagaagaaga aattgggtgt
     2401 gatacggttt tgaaaattgg tcgtatcttg attctctata aggtttctgc taagaaagaa
     2461 aaccgcaagt tgtcccctaa agttaaagca atctagtcct tttatttgta tagggactgg
     2521 ttttatgaga cgaggactat tgtcgcgaga attttggaga aagtatggca ttagaattgt
     2581 tgacgccttt tacgaaggtg gaattagagg aagaaaaaaa agaaagcaac cgcaaacaga
     2641 ttgggatttt aggagggaat tttaatccta ttcacaatgc acaccttgtg gttgcagatc
     2701 aagttcgcca gcaattagga ttagatcagg tgctattgat gcctgaatgt aaaccaccgc
     2761 atgtggatgc caaagagact attgatgaga agcatcgtct tcgcatgttg gaattggcta
     2821 ttgaagatgt tgaagggcta gctattgaaa cctgtgaact tgaacgtcag gggatcagct
     2881 atacctatga tacgatgctt tatttgactg agcagcatcc tgatgttgat ttttatttta
     2941 tcatcggagc tgatatggtt gattatttgc caaagtggca ccgtattgat gaactggtca
     3001 aactcgttca atttgttgga gttcaacgtc caaagtataa agctggaacc tcttacccgg
     3061 tcatttgggt agatcttcct ttgatggata tttcttctag tatgattagg gattttatta
     3121 aaaaaggtcg tcagcccaat tatctcttac ctaagcgggt tttagactac attactcaag
     3181 aaggtttgta tcaatgacat atgaagatta tcttccttat agcaggaccg aactgttagc
     3241 aaaaattgca gagcagatga gccctaaacg gtttaaacat gtacttggcg tggaaaaggc
     3301 agccttgtcc ttggcagagt gttatggttg taacccagac aaagcagggt tagcagctct
     3361 tttacatgat tatgccaagg agtgccctga ccaggtcttt ttagacttaa ttgacaagta
     3421 ccaattatct ccagaattag ctaaatggaa taataatgtc tggcatggta tggttggtat
     3481 ttataagatt caagaagacc tagggctaaa agacaaagat atcttgcgag ctattgaaat
     3541 tcacaccgtt ggtgctgctg agatgacctt gctggataag gtcttatacg ttgctgatta
     3601 tattgaagaa ggtcgcattt ttcctttggt tgatgacgct agaaaaatcg cgaagctcga
     3661 tttgaatcag gctgttgcct atgaaacagt gaacactgtc gcttatttag cctcaaaagc
     3721 gcagcctatt ttcccacaaa ctctggatac ctataatgcg ttttgcagct atttaaagga
     3781 atactaatgg ataatgatat gaggaataaa atgaaaaaag aagaactctt aaaaattgtt
     3841 gtggaagcaa ctgatgaaaa gcgtgctaaa gatattttag ccttggattt agaggggtta
     3901 actagtttga ctgattattt tgtgattgcg agtgcaacaa atagtcgtca attagaggct
     3961 attgcggata atatccggga aaaggtaaaa gaggctggtg gagatgctag ccatgttgaa
     4021 ggtaatagcc aagcaggctg ggtcttgctt gatttgaccg atgtggtggt acatcttttt
     4081 ttagaagatg agcgttacca ctataatctt gaaaaattgt ggtatgaagc gcctgctgtg
     4141 gctctagatg cctatttagc ttaatgttac aaagagacct tgtcaatacg acagggtttt
     4201 tgaggatgat aatgaaacat aattatgaaa aatttgcgtc agtttatgat gctgtgatgg
     4261 atgattctct atatgacttg tggacagatt tttcacttcg acatttacca aagtctaagg
     4321 gtcgaaatcg tttgttagag ttggcctgtg gaaccggtat tcagtcagtt cgttttgctc
     4381 aagcaggttt tgatgtgact ggtttagact taagtcaaga catgctggct attgctaaaa
     4441 agcgtgcgca atctgctaaa aagaaaattg attttattca aggtaacatg ctggatttgt
     4501 cacaggtggg gcagtttgat tttgtaacct gctattcgga ttccatttgc tatatgcaag
     4561 acgaagttga tgttggcgat gtttttaaag aagtttatga tgttttagca aacgatggca
     4621 tctttatttt tgatgtgcat tcaacctatc aaacggatga gtgtttccct ggttattcct
     4681 atcatgagaa tgcagatgat tttgcaatgg tttgggatac ctatgctgac gaggctcctc
     4741 actctgttgt tcacgaactc actttcttta tccaagaaga cgatggacgt ttttctcgct
     4801 ttgatgaagt gcatgaggaa cgaacgtatg agttattgac ttatgatatt ttgttggaac
     4861 aagcagggtt caagtcattt aaggtctatg ctgattttga agataaggag ccaactaaga
     4921 ccagcaagcg ttggtttttt gtagcatata aatagaagaa aaaagtagcc tttttagcta
     4981 taggatttaa tgaattatct tgaatgttac ttatatgaaa agagttcatt aggcattaaa
     5041 tgctattatc aaagcttaga agtattggag acattacgat tgacactatg tctgaaggtt
     5101 gctaccaact ttaaagaaag cattttctat tatacgttgt gcttaagcat ttgatttaac
     5161 tcttattagc cctataaaag actatttagt acaatttgag gagattctat gactgttacc
     5221 ggaattatag ctgagtttaa tccgtttcac aatggacaca aatatttgct agaaacagca
     5281 gagggactaa aaattattgc tatgtcagga aattttatgc aaagaggaga gcctgctctt
     5341 attgataaat ggattcgatc agagatggct cttaaaaatg gtgcggatat tgttgtggaa
     5401 ttgccctttt tcgtttcagt tcaatcagca gactattttg ctcaaggagc tattgatatt
     5461 ctttgtcaat taggaattca gcaattagca tttgggacag aggatgtgat tgattatcaa
     5521 aaactgatta aggtctatga gaaaaaatca aagcaaatga ctgcctattt atcgacgctt
     5581 gaagatacac ttagctatcc acaaaaaaca caaaaaatgt gggaaatctt tgctggagtt
     5641 aaattttcag ggcaaacgcc aaatcatatt ttggggttaa gttacgcaaa ggctagcgct
     5701 ggaaagcata tacaactatg tcctatcaag cgtcaagggg ctgcctacca ttcgaaagac
     5761 aaaaatcatc tgctagcttc tgcaagtgcc attagacagc acctgaatga ttgggatttc
     5821 ataagtcatt cagtaccaaa tgcaggttta cttataaaca atccccatat gtcatgggac
     5881 cattattttt cattcttaaa atatcaaatt ctgaaccatt ctgatttaac tagtattttt
     5941 caggtcaatg atgaattggc aagtcgtatt aaaaaagcga taaaagttag tcaaaatatt
     6001 gaccacctag ttgacactgt tgctaccaaa cgctatacaa aagctcgtgt aagacgtatt
     6061 ttaacctaca ttttggttaa tgctaaggaa cctactttac ctaaaggaat tcatattcta
     6121 gggtttacct caaaaggaca agcacattta aaaaaattaa aaaaaagcag gcctctgatt
     6181 actagaattg gagcagaaac ttgggatgag atgacacaaa aagcagacag catctatcaa
     6241 cttgggcatc aggatatccc agaacaaagt tttggaagaa tacctattat aataaaaaat
     6301 gagaggctca attaaactct aattgagttt ttttgggggc ttgactctaa cattacgttg
     6361 atggtttata cttcagttaa tccaatgagg aaattttata acaataatta aaaaagtcag
     6421 tcaaaagata tgctttcgaa gaacaggcta acgtttttac aagattatat cagtaatcat
     6481 tttgacaatt gtagtaatgg aatgttgcca aaatttagaa tgttgtgtgt tatgaatgac
     6541 aagattttaa aggaatacta attaaacggg aggtcgtgaa ccggaagctg ttgtcactca
     6601 agcgccaaaa tctgtctgta acataatatt gatgtatggg aacgcaatta tttggttttt
     6661 attgtgatgc ttaaaacgat tttatatttc tattttacca aagcttttca aagtgctata
     6721 ataagggagt tagagtcaat attagtaacg ttaatgttga caatattcaa taaaatacta
     6781 ttaaaaggag acaaaaatgg gacgtaaatg ggcaaatatt gttgctaaaa aaactgctaa
     6841 agatggagca acatcaaagg tctatgccaa atttggtgtt gaaatttatg tggctgccaa
     6901 acaaggcgag ccagatccag agttaaatac agctcttaaa tttgtcattg atcgtgcaaa
     6961 gcaagcacaa gtgccaaagc atgttattga taaagcgatt gataaagcta aaggaaacac
     7021 agatgaaact ttcgtagagg gacgctatga aggttttggt ccaaatggtt caatgattat
     7081 tgtggatact ttgacatcaa atgttaaccg tacagcggca aatgtacgta cggcttacgg
     7141 taagaacggt ggcaatatgg gagcttcagg atctgtatcc tacttatttg ataaaaaagg
     7201 ggtcatcgtt tttgctggtg atgatgctga cagtgtcttt gaacaattac ttgaagcgga
     7261 tgtagacgta gatgatgttg aagcagaaga gggaacaata acagtttata ccgccccaac
     7321 agatcttcat aaaggtatcc aagcacttcg cgataatggt gtagaagaat tccaagttac
     7381 tgaacttgaa atgattcctc aatcagaagt agtattggaa ggtgatgacc ttgaaacttt
     7441 tgaaaagctt attgatgcac tcgaatcaga tgatgatgtt caaaaagtgt atcataatgt
     7501 agcagatttt taaattgaca tagaataata aaaagagttg aaaactattt cagctctttt
     7561 taagtgatgc ttttataagt aagtaccaag taacatagtc gttgttagaa gataatgaac
     7621 aatagcaaat cagtatcaac ttgataggaa tcaattatta gtgaactcta attaaaaatg
     7681 gtaaactaga cacaagaaca aaaaggagag ataggatgat taaaaaacaa tatttaggaa
     7741 gggcaacaat tgccctagca tcaacccttg tactagcagc ttgtgggtca tcaaaaacag
     7801 ctgagtcagg aaatcagggg agttctaaag aagtcttatt tgcaacggtt ggaacaacag
     7861 ctcccttttc ttatgaaaaa ggtggtcaac taactggcta cgatatcgag gtggctaaag
     7921 cagtctttaa aggatcggat gattacaggg tgtcctttaa aaaaacagaa tggtcttcta
     7981 tctttacagg tttggactca ggaaaatacc aaatgggtgg taacaatatt tcctttacca
     8041 aagagcgttc agctaagtac cttttttctt atccaatcgg atcaacacct tctgttttag
     8101 ttgttccaaa agacagtgat atcaagagct ttgatgatat ccaagggcat acgacacaag
     8161 ttgttcaagg gactacaagt gttgcacagt tagaagactt taataagaaa cacagtgaca
     8221 acccggttac tctaaaattc accaatgaga acattaccca aatgttaacc aatttgagtg
     8281 aaggcaaggc agatttcaag atttttgacg ctccaacggt taacgccatt attaaaaatc
     8341 aagggttaga taatctcaaa acaattgaac tcacctcaac agagcaacca tttatttatt
     8401 ttatctttag tcaagatcaa gagaaattgc aaagctttgt taacaagcgt attaaagagt
     8461 tgactgctga cggaactctt agcaagcttg ccaaagagca ccttggcggt gattacgtgc
     8521 catcagataa agagttgaaa cttcctacag ctaactaaac catctattaa ccagttattc
     8581 atctagaaga ctggtttttc tgatcggaaa caatattata gtttatgagg ttatgttata
     8641 aaaattgcaa atagccttaa tcaaaatgag atgttaaagt gctttttgag aagtgaatat
     8701 tgtaagaagt atgctttggg ttttagtcct caaataccaa gttttttgtt gactaaacaa
     8761 ataaggatgg ttagcatagc atatagttta aaactatggt atatgctagc cattatcaat
     8821 ttgaaaacta tatctaatta agatacaata atgatcgtaa acaaaatgaa tcgaggataa
     8881 cgctatgtct tttaaaaaat ggattggaat agtcggaatc gctttagcat caactgtatt
     8941 ggttgcttgt tctgaaaaac aagatgataa aaacactcta actattggag tgatgacaaa
     9001 aacagaatct gatcaagcga gatgggataa agttgaagaa ttattgaaaa aagacaatat
     9061 cactttgaag tacaaagaat ttacagatta ctcgcaacct aataaggcag ttgctaatgg
     9121 tgaagtcgat attaatgcct tccaacacta taatttctta aataactgga ataaggaaaa
     9181 taaggaacat ttggtggcca ttgctgatac ctatatcagc ccaattaacc ttttctctgg
     9241 aaccagtcaa gatgggaagg ctaaatacaa atcagtagct gatctgccaa atggtgctca
     9301 aattgcagta ccaaatgatg cgaccaatga aagccgtgcc ctctatgtgc tgcaatcagc
     9361 tggcctcatt aaattgaatg tgtctggtga tcaattagca accattgcca atatctcaga
     9421 gaacaaaaag aaattagata tcaaggaatt agatgctagc caaacggcac gtgctttagt
     9481 atctgctgat gcagctgttg tcaataatag ttacgctgtt cctgcaaaaa ttgactacaa
     9541 aacctctctt ttcaaagaaa aagcagatga caattcaaaa caatggatta acattattgc
     9601 aggtcaaaaa gattgggaaa aatcagagaa ggctgatgct attaaaaaac tcatcaaggc
     9661 ctaccaaact gatgaggtga agaaagtcgt ggaaaaaact tcaaatggta tagatgtatc
     9721 cgtatggtaa attttagcca ttgttataac aagagtgtgg tcgtgtttaa aacacatgct
     9781 ttcttgcatg cttgaggaag agtttacgac taatttacct ttagaaagct atttaagacc
     9841 ttttcccttt taggatttag aaaccttggg aagactattg catgttatct taatagtcat
     9901 gattatgcag gcgatgaaaa cattgctatc gtagattact gtcgccatat cgtactaata
     9961 gaggagttat taaaatctta tgaatgaagc aataattcaa ttagatcata ttgatattac
    10021 cttccgtcaa aaaaaacggg ttatcgaagc tgttaaagat gtcacggttc atatcaatca
    10081 aggagatatt tatggcattg ttgggtattc aggtgccggg aaatcaactc ttgtgcgtgt
    10141 gattaacctg ctacaggcac caacaaacgg gaaaatcact gttgatgggg atgtgacctt
    10201 tgaccagggg aaaattcaat tgtcagctga tgcccttcgc caaaaacgac gtgatattgg
    10261 tatgatcttc caacatttca atttgatggc ccaaaaaaca gccaaagaaa atgtagcatt
    10321 tgcgctccgt cattcgtcgt taagtaagac tgagaaagaa cataaagtta tcgagctgct
    10381 agaacttgta ggtttatcag agcgagcaga taactaccct gcccagttat cgggtgggca
    10441 aaaacaaagg gtggctattg cgcgtgctct agctaacgat ccaaaaattt tgatttcaga
    10501 tgaggcaaca tcagcactag atccaaaaac cactaaacaa attttggcgc tcttgcaaga
    10561 attaaaccgc aaacttggcc ttaccattgt tatgattact catgaaatgc aaattgttaa
    10621 ggatatttgt aatcgtgttg ctgttatgca aaatggtgtt ttgattgaag aaggttctgt
    10681 ccttgatatc ttttcaaatc caaaagaagc cttgacacag gaatttatca caacagctac
    10741 agggattgat gaggctttag aaaaaattaa ccaacaagac attgttaaac atttgcctgc
    10801 aaatgccctt ctagctcagt tgaaatatgc aggaacttct acggatgagc ctctacttaa
    10861 tagcatttac cgtcaatttg aagtgacagc taatattttg tatggcaata ttgagatttt
    10921 agatcatatt ccagttggtg acatgattgt tgtcttggaa gggcaagctg aaaatattct
    10981 tgcggctgag aaagccttgc atgaagctgg tgttgatgtt agtattttaa agagaggagc
    11041 ctagatgtca cagttaattc aaacttattt gccaaatgtt tatgaactcg gatggtctgg
    11101 agatgctggt tggggcttag cgatttggaa tactttatac atgaccattg tgccttttat
    11161 tgttgggggt gctattggtc tcttattagg cttattatta gttttaacag gaccggacgg
    11221 tgttattgaa aacaaaacga tttgttgggt tattgataaa gttacctcta tttttagagc
    11281 cattcctttt gttattttga ttgctatttt agctagcttt acctatttgc ttttaagaac
    11341 aactttagga gcaacagcgg ccttggtacc tttaaccttt gcgacttttc ctttttatgc
    11401 ccgtcaggtc caagttgttt tttcagaatt agacaagggt gttattgaag cagcgcaagc
    11461 ttcaggtgct actttctggg atattgtcaa agtgtatctc agtgaaggtt taccagatct
    11521 tattcgtgtt tctacagtca ccttaatttc tttagttggg gaaactgcca tggcaggagc
    11581 tatcggtgct ggaggattag gaaatgtagc gatttcttat ggttataatc gttttaacaa
    11641 tgatgtgact tgggtagcga ctattattat tcttttaatt atttttgcta tccaatttat
    11701 tggtgatagc ttgactagaa ggtttagtca taaataaacg aaacaacacc ctaagttttt
    11761 acttagggtg ttgttgttta gatgatgtca ttagcttttc ttcgttttac ttagtataag
    11821 ccctattaaa aaaccaatag cagcaaagat aatccatccc attgagaatt gccctagtgg
    11881 aacagttttt tggaaaaaag ttacaacggc ttctggtaaa tgaaataatc cagtcatttg
    11941 tgatagtgtt aggagtgcat caaatatggc aggaataaag gttagaccaa ttgtagttcg
    12001 atagacaaca ggatcattat tgaagcattt ttgtgccaaa acaaggaaaa ttaaatcgac
    12061 tgttaatggg tataaaagga ataaaacagg agctgaccag ttgataataa ctgataaacc
    12121 accaaaataa aagaaagctg atagtaaagt aaagatagtt gcccaagcaa tatgagataa
    12181 agcaggcact aatttatgga agtattcagc acttgacgtg attaagcctg ttgaagtggt
    12241 tagacaggcc aggaaaatca caacagctag aaatgcttgt ccgatgccac ctaggtaaaa
    12301 acgagaagca tgacttaaga tttgaccgcc attaactgga ttaccatgaa gggtaaagct
    12361 gccatcacta aaaggaaata atgattgtga tgttgctcca atacgaccga caaagatata
    12421 gacaagtgct aatagcaaaa tggcaatagc cccagaaata agtgttattt tggtcacttc
    12481 tttgtccgtc ttagcgccaa attgtttggt agcctcaatg actaaaatag caaaaacaag
    12541 agaagctagt gcatccatag tgccataacc ttgaattaat cctgctataa aaggaaaatc
    12601 tttaaaggca ttattaacac cgaccccagc gttaaaagca tcaccgtaat ttccagcagg
    12661 atggacaaag gacgcgataa ccaaaataga aataacaact aacaacgttg gcgttaaaaa
    12721 ttttccgata ttttcagcta gcttactagg tttgatagca aggaagtagg acagaccaaa
    12781 aaatagtata gcataaatag ctttattggt tgtattatct cctaagatag gagcgatacc
    12841 gactgaaaaa gaagtggctc ctgttcttgg aatagcaaaa aatggaccaa tagataaata
    12901 caaaatggaa gaatagaaga ttgcatacca tttagagatg gggcgtgcca aactttcaac
    12961 atcgcctgat ccagatttag caacagcaat gacaccgagt aaaggcaagg agacaccagt
    13021 taaacaaaaa ccaatgatag accatagaat ctgatgacct gagtagatgc ctaaaaaagc
    13081 tgggtaaatg aggtttgctg ctccaaaaaa tagtgcaaat agcatgaatc caatgacgat
    13141 atatacattt ttttgtttca tttataaata gtctttctat tgatactaat ccgaagattc
    13201 taacaatttt aattatatta tgtgtcaaaa taacaacaaa ggttattata tctaagatta
    13261 cttcgatagt caatagaaat tgactgaaaa ttcttagcac aaaaaatggt taagatatgt
    13321 agctcattcg atttgacgaa tagatagaat tatattatta taaaaaacat gtaactttag
    13381 taaggatgta acgtatgaaa aaaatatatg atttatgggt tagggttagt ctaatcaaaa
    13441 aaataggtat cggtgttgta ataggagtca tgttgggcat tttggctcct gatcttacag
    13501 gttttagtat tttaggaaag ttatttgttg gtggtttaaa ggctatcgca cctttgcttg
    13561 tatttgcttt ggtttcccaa gccatctccc atcagaaaaa agggaaacag actaatatga
    13621 cattaatcat tgttctgtat ttatttggta cctttgcgtc agctttagta gcagtactga
    13681 cagcttatct attccctttg acgttagtgt taaatacacc tgtaaataca gaattatcac
    13741 cacctcaagg tgttgctgaa gtttttcagt cacttctatt aaaattagtg gataatccga
    13801 tcaatgcttt agctactgcc aattatattg gtgtgctatc atgggcaatt atttttggac
    13861 tagcattaaa ggctgcaagt caagaaacaa agcatcttat aaaaacagcg gcagaagtta
    13921 cttcacaaat agttgtttgg atcatcaatc tggcacctat tgggattatg agtctggtgt
    13981 tcactacgat ttctgaaaac ggtgttggaa tcttatctga ttatgctttt ttgatactag
    14041 ttttagtagg aacaatggtt tttgtcgcct tagtggttaa tccacttatt gcggttttga
    14101 ttacacgaca aaatccttat ccccttgtct tgagatgttt acgtgagtca ggtcttacag
    14161 catttttcac acgaagttct gcagccaata ttccagtcaa tatgcaatta tgccaaaaaa
    14221 ttggattgag taaagatact tattcagtgt caattccact tggagcaact attaatatgg
    14281 gtggggcagc aattaccatc aatgtcctaa cgcttgctgc tgtacacact tttggtattc
    14341 ctattgattt tctaacagcc ctcttactta gcgttgttgc tgctgtctct gcttgtggtg
    14401 cttctggtgt tgctggggga tcgttgttac ttattcctgt agcatgtagt ttgtttggca
    14461 tctcaaatga tcttgcgatg caggtggtta gtgttggttt tattgttggt gttatccagg
    14521 attcttgtga gacagcctta aattcgtcaa ccgatgttct ttttacagct attgctgaaa
    14581 atgctttttg gaaacgtaaa aaagcctagc taggtttaac atagtaaaaa acatccttgg
    14641 aaaacgccaa ggatgttttt ttattattta agatagccaa gataatcaaa tttttcaaag
    14701 gtgtggtcat tagcgatggc tacaatgata gtattcggtt ctaaaggttc aaaaggtttg
    14761 acattggtat ctaaggtttt aacttctttt tgacgcatgc caatcacatt taactcatat
    14821 ttacgacgaa catctagttc ggaaagagat tgtccttccc agcttttagg aatgacaaat
    14881 tcaatcatgg aaatgccatg ttcgaggtag atgatgcttt cgatatgacg tcttaacaga
    14941 tttgaagcta cacgttttcc agaatcacgt tcaggggtga tgactttagt tgccccgatg
    15001 ccatatagaa cttcttcaaa aattttattt ttagccttgg caataatggt tggaacaccg
    15061 agttttttac agtgcatgac tgcaagaaca gaagattcca agttattccc tgaagcgata
    15121 acgacagtat cacaatgttc aatccctaca gctaatagaa attctttgtc agtaatatct
    15181 ccaacagcgg ctttagtgac caagtcggcc acctctttaa catgactttc tctgatatca
    15241 atagcaataa cgtcttgatc aaaattactt aattctctgg caacagtacg gccaaaaata
    15301 ccaaggccaa gaacgccgac agttttacgt tttaacatgt tttctcctta acccactaaa
    15361 atatcagtag tggcatattg aatggttttt tcttttcgtt gaatcagact gatgaggaca
    15421 gtaattgggc caacgcggcc gacgaacatc aagacaatga caataagacg tccagcagta
    15481 gacaattgtg gtgtcaaatc catagatacc ccaactgtcg cgatggcaga aatagattca
    15541 aataagagtg gtataggagc gatatgaggc tccacgctaa gtaataaaat aaaacctatc
    15601 ataagaactg caaaaaagaa gataagaact gtcatggtct gcttaattgt cttatttgcg
    15661 ataattcggt tacgaaaggt aacttctgat tgccctgaaa gttccgcttt aaagagtaag
    15721 aaagtaatgg cagcagtcgt cactttaata ccaccagctg ttccaccagg agcaccacca
    15781 ataaccattt gaatcatata aagaatgtta gtcggtgcta gggtatcgtt ataagagatt
    15841 gttgcaaagc cagctgttct catagtgact gtttgaaaaa atgaaaccat aagttgctga
    15901 tgtaaactaa aattggcaat ggttttgctg ttatcttttt ctaaaaacca ggttaagaag
    15961 gttccaagaa aaagaataac agctgtggtt tgtagtacta gacgagactg attggataat
    16021 ttacgaaaag ttgccccata acaatgaggt ctttcaaaaa agtatttttt aaaagctact
    16081 cctaaatcaa cccaaaccgc aaaaccaagt ccaccagaga taatcaagaa cgtaatgata
    16141 acatttaatg taggatttaa cataaagtct tttaaactag aagatcctaa attgtcaaaa
    16201 ccagcgttac aaaatgccga cacagctaga aaaatactat tgaagatacc atttttccac
    16261 ccgaagcggg ggataaagtc aatcatgata actatcgcag caaaggcttc tatactaaag
    16321 gttactttat aagcaaaaaa aaggtagtgc tttaaatctt tgctatcacc tcgatttaac
    16381 gctgattgga gcaaggtttg atcgctcaat cgcatttttc gcttgagggc aaaagtactc
    16441 acagctatta acgtcaccaa gccaagacca ccaatctgca ttagtgccat agcaatggtt
    16501 tgtccgatac cgttatacac ttcagcgacg ggaacaacag aaagtccagt cacacaaacc
    16561 atggaaacca cattaaagaa atgatcaaga tagacggtat taggaccatt ttgataatgg
    16621 gtaaagggca tggataacaa gagagtccca atcagaatca caatggcaaa actaaatgtt
    16681 aagcgctgtg tgactgaaag agatttgata aaagagcgtt tcatgtttcc tccagacata
    16741 atatctccta ctataataac aaaaataagt taaaaaagct aagtttcatc ttagcctttg
    16801 gtactatttt atagaggttt tttgttaggc aagccagctt ttcttggata cttgttaggt
    16861 gtttcctttt tcttctcaac aatggtgatg aagcgagaat ctccatttgg caattggtag
    16921 ctatggtttt taatgacttt accaaaaaga agacaaagag cattcttagc ttcttctaac
    16981 tcttggtcag cagcttgtgc ctttaaggcg attaattttc ctccaatttt taaaaagggg
    17041 atggttaatt cagatagaac ctgcatccga gcaaccgctc tggcagtaac gacatcaaat
    17101 tgacctcgaa aggccttgtc ctgtccgaaa tcttctgctc taccatgaaa aaaatggaca
    17161 ttttccaagc ccaattcctg agcaaggagt gtcagaaaag aaatccgctt attaagagaa
    17221 tcaataatgg taacctcaag gttcgggaaa agaattttca tgggtaggct aggaaaacca
    17281 gctccagctc caatgtccaa taatttgatt ggttcattag ctaaaaagcc ctgtaaaata
    17341 ggagcgatgg aatcataaaa atgcttgagg taaacctcat tttcttctgt aatagctgtt
    17401 aaattaattt tagtgttcca ttccactagt aatttaaagt aggtgtcaaa ttgttctttt
    17461 tgtttcgatg ataaggaaaa accatcttcc tctagtgttc ggtaaaagtc ttggggtgtc
    17521 atttgtatcc tctgtttttg atagttgtta ccattttatc ataaaatagc ccataacaaa
    17581 atccaataaa tcatcagaaa taacacctca ttacttgcta ttagcctaga aattgctata
    17641 attgagacac aaagattaaa ggagacccac tatgccaaca gttttgatta tcttagtcgt
    17701 tttaggagtg cttgcgctat ggttaatgat tagttacaat agcctggtta aatctcggat
    17761 gcacacaaaa gaagcttgga gtcagattga tgtgcaatta aagcgtcgta atgacttgat
    17821 tccaaatctt attgaaaccg taaagggata tgctagctac gagcaaaaga catttgaaaa
    17881 aatcactgat ttgcgtgcgc gtgttgcgaa tgcctcaact cctcaagaaa ccatggcggc
    17941 ttctaacgaa ttgagtaaac aagtgaccag tttgtttgcc gttgctgaaa attacccaga
    18001 cttaaaagct aacgaaaact tcttgaaatt acaagaagag ttgaccaata cggaaaataa
    18061 aatctcatat tctcgtcaac tctataattc aacaacgtct aattacaacc ttcaattaga
    18121 atctttccca agcaatatcg ctggtaaatt atttggtttt aaaccaagtg aattcttaca
    18181 aacaccagaa gctgaaaaag aagttccaaa agttgaattt aacttttaaa tatgaaatta
    18241 tcatctagta ggcaatcata agagaaagga gaattatgct ttatcaacag atttcacaaa
    18301 ataagcaacg aacagttgtt ttattagttg ttttcttcgc tctcttagca cttattggag
    18361 cttcagcagg ctatctgctg ttagataatt atgccatggg cttggttctt gctcttgtca
    18421 ttggggtgat ttatgctacc agtatgattt ttcaatccac cagtcttgta atgagtatga
    18481 acaatgctag agaagtcaca gaaaaagagg ctccaggctt cttccatatt gtcgaggata
    18541 tggctatggt ggctcagatt ccgatgccga gagtttttat tattgaagat ccttctttaa
    18601 atgcttttgc gacaggatct agccctcaaa atgctgctgt tgcagcaacg acaggtttac
    18661 ttgaagtcat gaatcgtgaa gaacttgaag gtgttatcgg ccatgaaatt agccacatcc
    18721 gaaattatga tatccgtatt tcgactattg ctgtagcttt ggcgagtgct gttaccgtta
    18781 tttctagtat cggtgggcgt atgctatggt atggtggcgg ttctcgcagg caaagagatg
    18841 acggagatga tgatgtttta agaatcatta cattactttt gtcgttatta tctttgcttt
    18901 tagcgcccct agtagcaagt ttgattcagt tggctatttc tcgtcaaaga gaatatctag
    18961 ctgatgctag ttctgttgaa ctaacaagaa atccccaagg tatgattaac gctcttgaaa
    19021 aattgcagtt atctcagcca atgaagcatc ctgttgatga tgctagtgca gccttgtata
    19081 ttaatgagcc tcgcaaaaaa aggagcttca gttcattatt cagcacccat cctcctattg
    19141 aggagaggat tgaaaggcta aaaaacatgt gagcgtttgg ggtttttgct caaacctcac
    19201 ttttttatgt ctttgggcag ataatctaaa atggcaggaa ttatcaaaaa tatctgaaca
    19261 aaaagaaaaa aatgagtgaa acaatggtta aaaatagaat gatttaaatc ttaaaattaa
    19321 aaaacgtcag aaaataaatg caaaactatt gacaaatctt ttagatagta gtaaaaataa
    19381 atgagagttt tattaggaga agatgatgtt agctatttcc gaaatcagaa aacacccaga
    19441 cggtctctcg tttgatcgtt tatgtgatgt caaaagtatg ttacttgagc gtgaccaaca
    19501 gattattgac ataaaggcag ttaaagctgt cggtaatgtt cgctatgata aggggcttta
    19561 tctgttagat tatcaattga gttacgaagt gatattaccg tctagccgtt ctatggttcc
    19621 ggtctgttta agtgaagtcc aacacattca agaattattt atagaggcga cagatttagc
    19681 tgataagaaa gagttggttg aagacaatct ggtattagtt ttagacaaag acgctattaa
    19741 cctcgaagaa agtattgtgg ataatatctt acttgctatt ccgctacagg tcttgacaga
    19801 ggaagaaaag aaatcaaaag agttgccagc aggtcaaaat tgggcagttt tgacagaaga
    19861 agactaccag tgtctaaaag aagaaaagca aaaagaaaat aatccttttg ctagcttgca
    19921 agggttgttt gatgaataaa actgaatatt aaagatgtct gtaattttta tttgaaaaaa
    19981 cagctattct ttgatatact ctttagagaa tatggttact tatctaaagt agaaggtatt
    20041 ttgaccatag agggcaaaga aggtaatgac tattagatgc ttcatatttt attcgctaga
    20101 agactatttg accattagaa ctgaaatagt ctaggatatg agatgaattt catagttttc
    20161 tttctgggag aaaaagatag attaagagga taagggttgg tataaatgac aaagaaaatt
    20221 ttaattattg aagatgaaaa gaatctggct agattcgttt ctcttgagct gcaacatgag
    20281 ggttacgaag tcattgttga ggtcaatggt cgtgaagggt tagaaactgc tttggaaaaa
    20341 gagtttgatt taatcctgct tgacttaatg ttaccagaga tggatggttt tgaagtgacc
    20401 cgtcgtttgc aaaccgaaaa aacaacgtat atcatgatga tgactgcgcg tgattctatt
    20461 atggatgtgg ttgcaggttt agaccgcggt gcagacgact atattgttaa accgtttgcc
    20521 attgaagaac tacttgcccg tgttcgtgct attttccgcc gtcaagatat tgaatctgag
    20581 aagaaagtgc ctagtcaagg catttatcga gatctagttt taaatccaca aaaccgttca
    20641 gttaatcgtg gcgacgatga gatttctctc actaaacgtg aatatgattt gcttaatatt
    20701 ttgatgacta atatgaatcg tgtcatgaca cgtgaagaat tattgtcaaa tgtttggaaa
    20761 tatgatgaag ccgttgagac taatgttgta gatgtctata ttcgttatct ccgcggcaaa
    20821 attgacattc caggcaagga atcttatatc caaacagtgc gtggcatggg atacgttatt
    20881 cgtgagaaat aagtcatatg gaaaatcaga aacaaaaaca gaagaaatat aaaaattcgt
    20941 taccaaaacg actatctaat atcttttttg ttcttttttt ctgcattttc tctgccttta
    21001 cactgattgc ctatagttca acaaactatt tcttattgaa gaaagaaaag cagtcagtct
    21061 ttcaagctgt aaatattgtt agagttcgtc tttctgaggt ggactctaat tttacattag
    21121 agaacttagc agaagttttg tacaaaaacg ataaaacaca tctgagaatt gatgacagaa
    21181 agggcagtcg agtcattagg agtgagcgcg atatcacaaa tactctagat gcgaatcaag
    21241 atatttatgt ctataacatt gataagcaga tgatttttac cacagataac gaagaatcat
    21301 ctcctggctt gcatggtcct atcggtcggg tgtatcacga ccatatcgaa gatcagtatc
    21361 gtgggttttc catgacacaa aaggtatatt ctaatcggac tggaaaattt gtgggctatg
    21421 ttcaagtctt tcatgattta ggcaattatt atgtcattag agcaagactg ctgttttggc
    21481 tactagttgt tgagttattt ggcacaagct tagcctattt aatcatctta attactacgc
    21541 ggcgcttttt gaagccttta cacaatttac atggagtaat gcgtaatatc tctgaaaatc
    21601 ctaataattt aaacctgcgc tcagatattt cgtcaggaga tgaaatcgaa gaattgtctg
    21661 ttatctttga caatatgtta gacaaattgg agacacatac taagttgcaa tcacgtttta
    21721 ttagtgatgt cagccatgaa ttacgaacac cggttgcgat tattaaaggg catattggtc
    21781 tcttacaacg ttggggtaaa gatgacagtg atattcttga agaaagtttg acagcaacag
    21841 cgcatgaggc tgaccgtatg gcaatcatga ttaatgatat gttagacatg attcgcgtac
    21901 aaggctcatt tgagggacat caaaatgata tgacagtttt ggaagattct attgaaactg
    21961 ttgttggtaa ttttagagtt ttaagagaag attttatctt tacatggcag tcagaaaatc
    22021 caaaaacgat agcccgtatt tataaaaatc attttgagca ggctttgatg attcttattg
    22081 ataatgctgt gaagtattcc cgtaaagaaa agaaaatcgc gattaacctt tcagtgactg
    22141 gcaaacaaga agctattgtt agagttcaag ataaaggcga aggaatttct aaagaagata
    22201 ttgaacatat ctttgaacgc ttttatagaa cggataaatc acgtaatcga acaagtactc
    22261 aggctggatt agggattggc ttgtctattc tcaagcaaat tgtagatggg tatcatttac
    22321 agatgaaggt tgaaagtgaa ttaaatgaag gttcagtgtt tatcttacat attcctttgg
    22381 cccagtctaa agagagttag agtagcttaa ggcattgtta atgtgacagg tatagtatgt
    22441 aatcattaga taatagtagc agtcttttgc cacaactggt tagaagctga tgctattttt
    22501 ttagcaatga tggatgacat gaaagaaata ctgtcaatcg cgatgccttt tgttatcctt
    22561 gataatatgg taaaatagtg tattagatta atttgataga attggggtgt ttgcgtgcgt
    22621 tgtccaaaat gtaattacca taaatcgagt gttgttgaca gtagacaagc tgaagacggc
    22681 aacaccattc gtcgtcgaag agaatgtgaa cagtgtcata ctcgctttac tacctttgag
    22741 cgtgttgaag aactcccttt attagtcatc aaaaaagatg gcacacgaga gcaattttct
    22801 agagataaaa tattaaatgg ggttgtccaa agtgcgcaaa aacgacctgt ctcgagcaca
    22861 gatattgaaa atgtcatttc tcgtattgaa caagaagttc gaacaaccta cgaaaatgaa
    22921 gtgtcaagta ctgctattgg taatttagtg atggatgagt tggccgaact tgatgaaatc
    22981 acatatgtac gctttgctag tgtctacaag agcttcaagg acgttgatga aatcgaagaa
    23041 cttttgcaac aaattacgaa tcgcgtacgc ggaaagaaaa aacgtttaaa taatgatgaa
    23101 accaattgat acttttacat atctaaaacg caacaaagta actcttgatt cggtgacttt
    23161 aatacagttg tactttccaa ttattggaag tgacgcagtc tctatctatc aatactttat
    23221 tcactttttt gacgatggtt tacaacgtca taaattcagc gatattttaa atcacctaca
    23281 gtttggtatg aaacgttttg aagatgctct tgccatattg acagcaatgg aattagtgag
    23341 tgtgtatcag ctatcagata catatctcat tacgttgcac cagccgctta gccgtgactt
    23401 gttttttcaa catccagctt attctcgatt attagaacaa aagattggag aggtagctgt
    23461 ctctgagttg caggtaactg tcccaagtca agcaagaaat atttctaagc gattttcgga
    23521 tatttttggg gtgcaaggag atttgacgaa cgtacctcaa aagccacaaa agaattttga
    23581 cctttcaagt ttccagcaat taatggttcg tgatgggtta caatttgaag ataatcaaaa
    23641 agatatcatc agcctttaca gtattgctga acagtatgat atgacgtggt ttgatactta
    23701 tcaaatagct aaggcgacgg ctgttaatgg gaaaattcga cctgaacgct tattggctaa
    23761 aaaaaatcaa agcatgacaa aaccaagcaa agaaaatttt tcacaggctg agcaaattat
    23821 tttgcgagaa gcaaagcaag acagtgcttt agtctttttg gaaaaaatta aaaaggctag
    23881 acgtgcaacg attactaagg atgaacgcat tttattgcaa acattggcca aaatgaattt
    23941 tcttgatgat gttatcaatg tcatggtgct ttataccttt aataagacaa aatctgctaa
    24001 tttacagaag agttatgtct taaaaatggc caatgatttt gcctaccaaa aggtgtcaac
    24061 ggctgaagaa gctatagtgg ttttgcgagc cttcacggat cgtcaaagtc gtcggcaatc
    24121 aaaagtaaaa acaagtcaaa gcaatgtgcc taagtggagc aatcctgatt accaagagac
    24181 aacaagtcaa gaagagcagg cgaaactaga tcagttcaaa caggctgctt tgaagaggtt
    24241 agaaaaccta ggaaaagggg gtgactagat ggaaaagatt ggagaaacca tggcgaaatt
    24301 gggtcaaaac actcgtgtta atagcgacca gttgattcaa accattttag cggatcctga
    24361 agtagcaagc ttcatcagtc aacatcactt atctcaagag caaattaacc ttagcttatc
    24421 taagttcaat caatttttgg tggaacgcca gaagtatcaa ctcaaagatc cctcttatat
    24481 tgctaagggc tatcagccta ttctagctat gaatgagggc tacgctgatg tgtcttattt
    24541 ggaaaccaaa gaattggtag aagctcaaaa acaggcagct atctctgaac ggattcaact
    24601 ggtcagtttg ccaaaatcct atcgccatat tcatttacca gatattgatg ttaataatgc
    24661 gagccgtatg gaagctttct ctgctatttt agattttgtg gaacaatacc catcagcaga
    24721 gcaaaaaggc ttatatttgt acggagatat gggaattggc aagtcttact tgttggctgc
    24781 tatggcccat gaattgtctg agaaaaaagg tgtgtcaacg acccttctac attttccgag
    24841 ctttgccatt gatgtaaaaa atgccatttc aaacggctct gttaaagaag aaatcgatgc
    24901 ggtcaaaaat gtaccagtgc tcattttaga tgatattggt gcagagcaag ccacttcttg
    24961 ggtccgtgat gaggtgttgc aggtgatttt acaataccgt atgttagagg aattgccaac
    25021 cttctttacc tctaattaca gctttgcgga tttggagcga aaatgggcaa ccatcaaagg
    25081 aagtgacgaa acttggcagg ccaaacgtgt catggaacgc gtgcgttatt tggctagaga
    25141 gtttcattta gaaggggcta accgccgtta agtttgagat agcaatcaag aagaatagct
    25201 acacgtatga gtgagtgaaa gaaaaggaga atcatggttt tacctaccgt tgccattgtg
    25261 ggccgtccaa atgtgggcaa gtccacctta tttaatcgaa tcgcaggaga gcgcatttca
    25321 attgtagaag acgttgaagg ggttactcgt gaccgtattt atgctactgg ggagtggctt
    25381 aaccgccaat tctcattgat tgatacaggt ggtattgatg acgttgatgc cccgtttatg
    25441 gagcaaatca agcaccaggc tcaaattgct atggaagaag cggacgttat tgtctttgtc
    25501 gtttctggta aagaaggggt gaccgacgct gacgaatatg tgtctaaaat tctgtatcga
    25561 accaataccc cagtcatttt agctgttaat aaggttgata atcctgaaat gcgtaatgac
    25621 atttatgatt tttattcact tggtttaggg gatccctatc cagtttcttc tgttcatggt
    25681 atcggaacag gggatgtctt ggatgagatc gttgaaaact tgccagtaga agaggctgaa
    25741 gaaaatgatg atattattcg cttcagttta atcggtcgtc ctaatgttgg gaaatctagc
    25801 ttgattaacg ctattttagg tgaggaccgt gttatcgcta gccctgtcgc agggacaaca
    25861 cgtgacgcta ttgacacgca ttttacggat gctgacggtc aagaatttac catgattgac
    25921 acggctggaa tgcgcaaatc tggcaagatt tacgaaaata cagagaaata ttctgtcatg
    25981 cgtgccatgc gtgccattga ccgctccgat gtggtcttga tggttatcaa tgctgaagaa
    26041 ggtatccgtg aatacgacaa gcgtatcgct ggctttgcac acgaagctgg taaaggaatg
    26101 attatcgtgg ttaacaaatg ggataccatt gacaaggata atcataccgt tgctaagtgg
    26161 gaagccgata ttcgtgacca attccaattt ttgacatacg ctccgattat ttttgtgtca
    26221 gctttgacga agcagcgcct caacaaattg cctgacttga ttaaacgtat cagtgagagt
    26281 cagaataaac gtatcccatc tgctgtcctc aatgatgtta tcatggatgc tattgctatt
    26341 aatccaacgc caacggataa gggtaaacgt ttgaaaattt tctatgccac tcaagtgtca
    26401 gtaaagccac caacatttgt cgtctttgtt aacgaagaag aattgatgca cttctcttat
    26461 ctacgcttct tggaaaacca gattcgcgca gcctttacct ttgaagggac accgattcat
    26521 ctgattgctc gcaaacggaa ataagaaatg aacatcaact aaaatgctga cctaagaaat
    26581 aatctcatca ttgataagga ctgtgttgtg tatgttacag gagacagtcc tttttataat
    26641 acctttaagg gtgtagacgg aagattagta gcaaacaaag aaaaaggatt ttgacaagaa
    26701 atatcagtcg gtttttgcta taatgaaagg ataagcaaaa aggtggaaaa atgatggcta
    26761 gattaattcc aggacgagta aggaatgaag gtattaagct ttatgagcaa ggtttagtct
    26821 cattccaaga tgataataaa ggcattcttc aaatagaagt ggagacttat caggttcaat
    26881 atggagcaga tgatgaggat atcacttgtc aatgcgacac atttcatatg aaacattatt
    26941 gcaagcatat tgcagcagtc gagtattttt taaaaaatga tcaaaaaggt aagctttttt
    27001 taaaacagtt aacaaatcaa acaaaaatca aagaaacaac aaagaaaatg acatcttttg
    27061 gtagtctctt tttagatagc ttagccatga atgaagatga ttctgttaaa tacaggctgt
    27121 ctgctcttgg tagtcgaagc cctttttcgt cagattactg gtggagtctg aaaatcaatc
    27181 gtttaccaga tgatcgttct tatgtgattc gagatattaa aggctttttg cagcttatta
    27241 aaaaagaagg cttttatcag attggaaaaa attattttga acaactttca tggctgcagt
    27301 ttgacccatc cagtcaagac cttattgagt ttttatggcg cttagcatct aatactgaca
    27361 aaggggacaa cgagaacatt tttccaaatc atgcaaggca tttgcgctta cctagtggtt
    27421 tttttgaaga aggtattcac tatttgacgt cactttatga ctttactttt gaggggcctt
    27481 ctcaaactta tcatcatctt tttgtccgtt ctttggaagc agaggcaggc ctttatgagt
    27541 ttaaagtgga agtgcatcgc aaatctattg aattgcaaat tgctgaaaaa aatgtgcaat
    27601 acctctttga taatgactat cttctttatc aagatacctt ttatcacttg acgttaaagc
    27661 aacgaaaaat ggttcaggct attcgtagtc tgcccattga ggccgacttg gctaaacata
    27721 ttcattttga tttggatgat catgcgaaat tagctgcaag cttatcagat tttaaacaga
    27781 tcggtctagt agaggctcct aaaagctttg ctattagaga ctttgaggtg acttttcaat
    27841 ttgatttgtt aaatcgagat gaaatcagtt gtcagcttat gtttgattat ggcaattacc
    27901 aagttagtga caaagcaagt ttagaagcac ttccttttgc tagtcattta aaaaaagaag
    27961 aaaaaatcaa tcgttcttta cttgcttttg gcttctcgcc acaattttac tcaaaaaagc
    28021 gactaacaag tgcaaaagag ctctatactt tttttgaaga aacagttcca tgttttgagc
    28081 gcttaggtaa tgttgcgttg agtacagcta tccaagcatt acaagtgaaa gaaatgccca
    28141 agatcgctat tagacgcaat cagggattac tagatatttc gtttgatttt tcaaccatta
    28201 ttgaaaatga tattgaccaa gctgttacag ctctttttca aaataatcct tattttgtga
    28261 gtcaaacagg tcaattagtg gtttttgatg atgaaactca aaaagttagc aaaagtttac
    28321 aagaattaag agcaagacag ctaaaaaatg gtcacttgca actggatggc attagagctc
    28381 tacaagtatc caaacttttt gaaggcatga catctgttca tttttcaaag gaattagaag
    28441 agttagccta tcatttgcag catcctgaaa ctttttcaat aaaaccgtta ccagttaagg
    28501 ctcaaatgcg agattatcaa cgcaacggtg ttcagtggtt atcaatgctc aatcattatg
    28561 gctttggtgg cattttagca gatgacatgg gacttggaaa aactctccag acacttgctt
    28621 ttttagctag tcacctgaaa tcagatagta aagtgctcat tttatctcca tctagtctta
    28681 tctataattg gtttgatgaa tgtcaaaaat tcacacctca gctagatgtt gtagtttctt
    28741 acggacttaa acaaatcaga gatcaaatca tcgaagaagg tcatcaaata accataacca
    28801 gctattcctc ctttagacag gattttgaga cctatcaagc gtttcactac gattatctta
    28861 tcttagatga agctcaagtg ataaaaaatg cgcaaactaa gatttcacac tgtttacgtg
    28921 cgtttaatac agccaattgt tttgcgcttt cgggcactcc tattgaaaat aaaatgcttg
    28981 aaatttggtc cattttccaa attgttttgc cagggttgct accgactaaa aaagagtttt
    29041 taaaattaac agctgagcaa gtatcgcgct atatcaaacc ctttgtgatg cgtcgtaaaa
    29101 aagaagatgt cctaccagaa ttacctgatt taattgaaac taattattca aacgagatga
    29161 cagatgaaca aaaagctatt tacttagcgc agttaaggca gatgaaagat caaattcgaa
    29221 attctagtga tgtggatatt agccgtcaaa aaattgagat tttatctggt atcactcgat
    29281 tgaggcaaat ctgtgacact ccaagtctct ttatggatta ccaaggcaaa agtggtaagc
    29341 tagatagttt gcgaatctta ttgacacaaa ttaaagaaaa tggtcatcgg gctttgattt
    29401 tttctcaatt tagagggatg ctggacttgg ccaagcaaga gatgactgct ctaggattga
    29461 cctcttatca aatgaccgga tctacccctg ccaatgaacg tcaagaaatg actagagcct
    29521 ttaataacgg ttccaaggat gcttttttga tttccctcaa ggcaggtgga gttggaatca
    29581 accttacggg agcagacaca gtgattttaa tcgatttatg gtggaatcct gctgttgaaa
    29641 tgcaggcaat cagtcgcgcc taccgtattg gtcaaaaaga aaacgttgaa gtatatcgct
    29701 taatcactcg agggacgatt gaagaaaaga ttttagagct tcaagaaagt aagcgcaatt
    29761 tggtgactac agttttggat ggcaatgaaa accgagcaag tatgtccatt gaagaaataa
    29821 aagaaatttt ggggctaaac actcattaat cttccttgat tttagtgaca ttaaaaaccc
    29881 tttaaagaag atgacaccaa aagcacctta tataacctcg ttaaaacgct tctttttgaa
    29941 atatctttat tgatatagcg attgtcattt gctagtaatg attaaaacat ttagaaaaag
    30001 acaagtcata gaatcatcaa ggaatgcttt gaaaaataac agtatttaag ttataataga
    30061 ggatgctaca agaagggaga atcaaaaaag gatggtaaaa aatcagtttc ctttagttgc
    30121 agatggcatt gctattagtg atccagcaaa gcaaatggct ctgtatgaaa atgaagatct
    30181 cattaccaac atccgtggct attaccagga caaggagtat gatgatattg ctagaaatga
    30241 ggagtttact gctaaagcaa cttctcggca ggccccttct agtaagcgct tttgtagcaa
    30301 tgatgaaaaa caccattatg tcaaagaggc gcgtcaaaaa gcaaagcaag atctcaagga
    30361 aaaaagacag gcttatcttg ccaaagaaat ggcctatgtt cctaaacagg tttctaaaaa
    30421 gcaacagcct gcggattctt ctccttctca aaagcaagca acaacagaaa tgagtcgttt
    30481 tacaaaaaag cttcatcaag acaactatat tttagctgag ttgccaaaag aatataaaga
    30541 gccaaaaaat cttcctcaac aaggaactac aaaaaaaaat aattacgatt ttttaaaatc
    30601 cagtcaaatt tacaataata aagaaatgag acaacaacga gaaaaaacaa ttgcgcaaga
    30661 gcttaatctt agtaggtttg aggatcttcc ttaataaatc tactcagcgt atgcagatga
    30721 caaaggagtt aagatgtcaa aaacgtatca ttttatcgga attaaagggt caggaatgag
    30781 tgccttggcc cttatgttgc atcaaatggg gcataaggtg caaggtagtg atgttgaaaa
    30841 atactatttc actcaaagag gattggaaca agcgggcatc actatcttac cttttagcga
    30901 ggacaacatt acgccagata tggaacttat cgttgggaat gcctttcgtg aaaacaacaa
    30961 agaagtggcc tatgctttga gacaccagat tcctttcaaa cgttatcatg actttttagg
    31021 tgattttatg aaatcattta tcagttttgc agtagcaggt gctcatggta aaacttctac
    31081 aacgggttta ttgtcacatg tcttgaaaaa tatcacagac acatcttatt taattggtga
    31141 tggtactggg cgtggatctg ctaatgcaca gtattttgtt tttgaatcag acgaatacga
    31201 acgccatttt atgccttatc acccagaata ctctatcatt actaatattg attttgacca
    31261 tcctgattat ttcacaggaa ttgctgatgt gcgaaacgct ttcaacgatt atgctaagca
    31321 ggtcaagaaa gctttgtttg tttatggtga agatgacgaa ctaaaaaaga tcgaagcacc
    31381 tgcacctatt tactattatg gttttgaaga gggcaatgat tttatcgctt acgacattac
    31441 gcgtactacg aacggatcgg actttaaggt aaaacaccaa ggagaggtca ttggtcagtt
    31501 tcacgtacct gcttatggaa aacataacat tttaaacgct acagcagtaa ttgcaaactt
    31561 gtttgtagcc ggcattgata cggctctagt ggcagaccat cttaaaactt tttcaggtgt
    31621 gaaacgtcgt tttacagaga aaattatcaa tgacactatt attattgatg actttgctca
    31681 tcatccaacc gaaattgtgg caacaattga tgcggctaga caaaaatacc caagtaaaga
    31741 aattgtagca atatttcagc cacatacttt tactcgcact attgccttat tagaggattt
    31801 tgcttgtgct ttaaatgaag ctgacagtgt ttatttagcc cagatttatg gttctgcacg
    31861 tgaagtagat aaaggtgaag ttaaggttga agatttggct gctaagatta tcaaaccatc
    31921 acaagtagta acggtggaaa atgtctctcc tcttcttgat cacgataatg ctgtttatgt
    31981 ttttatggga gctggagata ttcagctata tgaacattct tttgaggaat tacttgcaaa
    32041 tttaactaaa aataaccaat aacatgtcct gccttaggca ggttttctca ttctaaagga
    32101 gtttgatatg ttgattagac aggttcaagg atccgatttg gaagttattg ctaccattga
    32161 aagtgacaat ttttcaccac aagaagcaac gacaagagca gttcttgaag agcgcatacg
    32221 gctgatacca gatacttttt tagtggcact tatcgatcag gaaattgttg gctatatcga
    32281 aggtccagtt gtcactactc ctattttgga ggattctttg ttccacgggg tgactaaaaa
    32341 tcctaaaact ggaggctata ttgctattac tagtttgtcc atagctaaac attttcagca
    32401 acaaggggtt gggacagctt tgttggcggc actaaaagat ttagtagtag cgcagcaaag
    32461 aacaggtctt attttgactt gtcatgatta cttgatttcc tattatgaaa tgaatgggtt
    32521 tattaaccaa ggaatttcag agtctcaaca tggtggaacc ctttggtacc aaatgatttg
    32581 gaaactttag aatcacagct gattcaactg ttggtttgtt aaaaataagt aacaaatgca
    32641 tcatttaatt gcaattagga tacttttaag ttataatggc ggttgattat atttaggagg
    32701 attggctttg accgatttta aggataaaga ccaacaagat cagcagcgaa gctttaaaga
    32761 gcagattctt gctgagttgg aaaaagcaaa tcaaatcaga aaagaaaaag aggaagaact
    32821 ttttcaaaaa gagttggaag ctaaagaagc agctaggaga acggcccagc tatatgctga
    32881 atataaaaga caagatgctt ttcaaaaaga gtctatagca cataacaata agacagctaa
    32941 acactttcaa gctataaaag gtgcggtaat gacttcagaa gcgcttaaac cgactttact
    33001 ttctgaaaaa gaaaactcat ctttaaaaac gacaaataag agagtcgtgc aggcaaatga
    33061 gcttcaagag actgcctcta aagaatctca agtaccgtta actattgaga aaggtcattc
    33121 agtgagacga aaattaagca aacgccaaca gactgagcga gctgctaaaa agatttcaac
    33181 cgttttgatt agttctatta ttataaccct tttggctgtt actctagcag gagcaggcta
    33241 tgtttatagt gctttaaatc ctgttgataa aaatagtgat gcctttgttc aagttgagat
    33301 accatctggg tcaggcaata aattgattgg tcaaattctt caaaaaaaag gtttaatcaa
    33361 gaatagcact gtttttagtt tttatacaaa atttaaaaac tttacaaatt ttcagagcgg
    33421 gtattacaat ctgcaaaaaa gtatgagtct agaagaaatt gctagtgctt tacaagaagg
    33481 tggtacagca gaacctacca agccatctct tgggaagatc ttgattccag aaggatacac
    33541 gattaaacaa atagctaaag ctgttgagca taatagcaag ggaaagacca aaaaagctaa
    33601 aacacctttt aacgagaagg attttttgga tttagtcaca gatgaggctt ttattcaaga
    33661 tatggtaaaa agatatccaa aattattagc aactatccca actaaagaaa aagctattta
    33721 ccgtttggaa ggttaccttt tcccagcaac ctataactat tacaaagaaa ctaccatgag
    33781 agaacttgta gaggacatgc tggcagctat ggatgctact ttggtaccct attatgataa
    33841 aattgctgct agtggtaaga cagtcaacga ggtattgacc ttggcctctt tggttgaaaa
    33901 agaaggttca acagacgatg acagacgtca aattgcaagt gtcttttata accgccttaa
    33961 tagcggaatg gcactacaat ctaatatagc tattttgtat gcgatgggga aacttggtga
    34021 gaaaacaacc ttggctgagg atgctactat tgacaccacc attaattctc cttataatat
    34081 ttataccaat acaggtctga tgcctggtcc agttgctagc tcgggggttt ctgcaattga
    34141 agcaacccta aatccagcct caacggatta tttatacttt gtggccaatg tccatactgg
    34201 tgaagtttac tatgcaaaaa catttgaaga acactctgca aatgttgaaa aatatgtgaa
    34261 tagtcaaatt cagtaaacac aaacatggtg cttaagcacc atgtttgtgt ttctgattcg
    34321 ttcaaaaaag aaaagagaga aaacaattat ggcagaaaaa acgtatccga tgaccctaac
    34381 ggaaaaagaa caacttgaaa aagaacttga agaattaaaa cttgtgcgtc gtccagaaat
    34441 agtggagcgt attaaaattg cacgttcgta tggagatctt tcagagaact ctgaatacga
    34501 tgccgcaaaa gatgagcaag cttttgttga aggtcaaatt tcaacgcttg aaactaagat
    34561 tcgctatgct gaaatcattg acagtgatgc tgttgcaaaa gatgaagtcg ctatcggaaa
    34621 aacagttatc gttcaagaag ttggaacaac agataaagat acctatcata ttgtaggtgc
    34681 tgctggagca gatatcttct caggaaaaat ttcaaatgaa agcccgattg ctcaagcttt
    34741 gattggtaaa aaaacaggtg acaaggttag aattgaatca ccagcagcta cttatgatgt
    34801 tgaaatcatc agcgttgaaa agacaaatta atcagatgaa gccattattt gcctaaccac
    34861 taagtgtttt ggttgctaat ggcttttcta gtatcttcaa atgaggttga ctgttaggaa
    34921 ggtcaagtat taatcgcatt gattatgagc caagaggact atagatcaaa gatcgttttc
    34981 gatggttatt taatgccaaa agaaaagacc agcaccttaa tgtactgacc ccaaaaagtt
    35041 ggacactata ttttaaggaa gtgtatgatc taaggaaact gacactaatg attgaccgtt
    35101 ttgcaagact ttattaatga tttcttgctt tattttaaat gaataagtct gatacgtttc
    35161 cttccttaac actatcgact ccatactgac ccatcagtca aacggtatat ctcaaacgac
    35221 tacggtttac tccaaattga ttggctagtt gaggccaaga aacacctgat tttttttaat
    35281 gctaaatttc tagcttatct tcatagctta atttcataca aaaacacccc aatcgttaga
    35341 tttttttgat atgtcccctt tcactagatg agtccagtaa aagggggcat atcgtttttt
    35401 gtctaacttt tggggtgcag ttcataattg tgctaggttt tttgtctttc ttaacgcttt
    35461 cgctgttttc cagcattacg attggatttt atttttttag gcaagttagc ctgttccatg
    35521 ttttgtgaag gcgtaacgtc cttacgagaa gaagttgatt ggtatgcttt aggcgggttt
    35581 ttagcgtatt cttctttaat ttgcttatgc aatcgaggtt ttaagaggta ggttgtgatt
    35641 aactgttgaa taatactaaa gaaaccacca actagccagt aaagaccaac accagcaggg
    35701 agtgagaagg acatgaagat catcatgata ggcatggtgt acatcatcgt cttcatttgc
    35761 tcacgttgtt cttctgaaac ggccatcatt gataaccatg attggaagaa gtaaagagca
    35821 gcgataattg ctgttaacac taaactacgg ctaccaagat caatacccat aaaggtgctt
    35881 gttgatactc ctttagtgta ttgagcggca aaatacatgg cagaaaagaa tggcatctgg
    35941 atgagaagag gtagacatcc aatacctcca agaggattaa tcccatgagc acgctgagca
    36001 gccattaatt ctgtttgggc agccattttt tcttcctgac tacttgcctg tttgatacgt
    36061 ttgttgattg gttcaaaaac aggtttcaag aaggccattt tctcagattg ataacttgct
    36121 ttccaagatt gatatagacc tagtggtaag ataagtgttc taacaataat ggttacgatg
    36181 atgatagcca gtccataacc tagtccagca ttgtttgcaa agtaatcgat aaagtatgac
    36241 atgggttttc caagaaattc ccaaatcata cctttgggat ttccgtgagc gtctcttccc
    36301 acacagcctg tcaaggtgaa tagaattgac agtgctagtc ctgaaaagag gatgcgattt
    36361 aatgttagtt tcaatgtgtt tatcctttaa ttttaatata cttatctatt ttacagtttt
    36421 tttcagaaat tcacaatagt tctgaaggcc gatttttact tgcctatttg aaagtcctga
    36481 aagtcttcaa aattagccat ggtgacatcc acataagtga cttttgccca tttggatggt
    36541 cctttacgaa cttcttgaat aaatgtggct attttatctg aatcttttga ttgggccagg
    36601 atttcaacgg tcccatcgtt gttattccag acacgaccat aaatatctcc tatatctaga
    36661 gctaaagtat gggtagcgta tcgaaaacca acaccttgta cacgtccaga aacaattaaa
    36721 cgtacttttt gcataagaag ggcctccttg cttttattat agcacatact tagcaggaaa
    36781 tctctttagc taattgtcta cttcctgtca cttttactaa agacaagaat acaaaaatga
    36841 gatagttagt agtactaaca gtaaaagagg tttgtctatt ttaaaaatag tatcaaaaaa
    36901 aggtctttta ggtttaaaac attgaactgc ccccaaattg accatctaag aagttgatgc
    36961 tttcaaatgc tgtcgcgtat ggtataatag gtgcatggtt attacatcaa aaacaaatca
    37021 actaattaag caaacaaaaa aattattgca aaaaaagcat cgaaaacagt cctatttgat
    37081 tgagggctgg cacctttttg aagaagccca aaaatcagga caagtttttc gccacatctt
    37141 tgttttagag gaaatggtgg agcgtctagc aggtgagcaa gaactcgtta ttgttagccc
    37201 acaagtgctc aaggagttga ccgactctcc tagtccacaa ggcattgttg ctgaggtgga
    37261 gatacctaag ttagcctttc catcagatta caaagggaaa tatttggttt tagaggacgt
    37321 ccaagaccca ggaaatcttg ggaccatcat tcgtacagca gacgctgcac ggtttgatgg
    37381 ggtctttctt tctgaaaaat cagcagatat ttacaaccaa aaaaccctac gttccatgca
    37441 aggtagccat tttcatttac caatttggcg aacagatgtc tatcagcttt gtcgagagct
    37501 ccaagagtat aaaactccca tcctagcaac aactctgtca aagaaatctg ttgactataa
    37561 aagcctcaca catcatgaaa ggttggcttt ggtgttggga aatgaaggcc agggcattag
    37621 tgctgaaatg gcagccctag cagaccaact ggttcatatt acaatgcctg gtcaggcgga
    37681 aagtttgaat gttgccgtag cagcaggaat cctcattttc agcttgattt aagctaatat
    37741 gatataataa ccaagtgggg tgattatgtt atgcattata cagaagataa agaatatatg
    37801 gaacacgtcg gtcatttgat cgctcatcct cgctttcaaa aattgagtca tattgttcag
    37861 caccaacatt caacgcgtct tgaacattct atcaatgttt cttattccag ttataaatta
    37921 gccaagcgat ttggttggga tgctaaaagt acagccagag ggggcttact tcatgatttc
    37981 ttttattatg attggcgcgt gaccaagttt aataagggtc atgcctgggt tcatcctcgt
    38041 attgctgtac gaaatgcgaa aaagttaaca gaactcaata aaaaagaaga ggatatcatt
    38101 ctaaaacaca tgtggggagc gaccattgct tttccgcgtt acaaggaaag ttatattgtg
    38161 acaatggtag ataaatactg ggctgtcaag gaagcagtga cacctttaag acaaaaatgg
    38221 tctaatcgac ggtttcttcg ccgcaagacc ctccaaagtc ataatcgtta agtaaaggag
    38281 ttattatgaa cgatcacgtt atttatacac aatcagatgt gggattaaat cagttttttg
    38341 ctaaaattta tagtctagtg ggaatgggag tgggtttatc tgcttttgtt tcttatttga
    38401 tgctttatcc atttagagaa aatttaatct ctattcttgt taatcagccg atgatttact
    38461 atggtgctgc gattattgaa cttattttag tttttgtagc cagtggtgca gcacgaaaaa
    38521 acacaccagc tgcattgccc atttttctta tctattctgc gctaaatggc tttaccctaa
    38581 gctttattat tgtggcatac gctcaaacga cagtttttca agcatttctt tcttcagcag
    38641 ctgtgttttt tgccatgtca atcattggtg tcaagactaa acgggatatg tcaggtctta
    38701 gaaaggctat gtttgctgct ttgattggtg ttgttgtggc tagtctgatt aatcttttta
    38761 ttggtagtgg gatgatgagt tatgtcatta gtgtgatttc cgtgctaatt ttctcaggtc
    38821 tcatcgcatc agataatcaa atgattaagc gcgtttatca agcaacaaat ggccaggttg
    38881 gtgatggctg ggctgttgct atggccttaa gcttgtattt agattttatt aacttattta
    38941 tcagccttct tcgtattttt ggaagaaatg attaacatta aaaagtgagt ataactactc
    39001 actttttaat gttaacagga taagtaaact gtaaggaaga aattcgagat agatataaga
    39061 tgaggctgct aaaagaccta gtgttaattt gtaaactctt tctttttttg gtatactatt
    39121 aaggattaaa tacgaggaga atatagatag atatgtcaac agctatttgg attttattac
    39181 ttattgttgc ccttggggta ggtgtttttg gtgggatttt tattgcccgt aaacaaattg
    39241 aaaaagaaat tggtgaacac ccacgtttaa caccagaagc tatccgtgaa atgatgagcc
    39301 agatggggca aaaaccaagt gaagcaaaaa tccaacaaac ttatcgtaat atcatcaaac
    39361 aatcaaaagc agccgtatcc aaaggtaaaa aataaaagct aattgttata ggggaaacct
    39421 ttgtgatagt tagaagtgaa cagtctttga tctcaatgtt cagtagcact tgtcttatca
    39481 gttagatttg tcaactgttc agggtttaaa aagaaactca gttggctatt tccaactgag
    39541 tttttcatat cgtcagtaag gaagtaaaca taatggatac aagaccaatt gggtttttag
    39601 attctggtgt gggaggtctg accgtcgttt gtgaattgat tcgtcagctc cctcatgaga
    39661 agattgtata tattggtgat tcagcaagag ccccctatgg tcctagaccc aaaaaacaaa
    39721 ttaaggagta tacttgggag ctagtcaatt ttttgctgac tcaaaatgtt aaaatgattg
    39781 tttttgcatg caataccgca acagcggtgg cttgggaaga agtaaaagca gctttagata
    39841 ttcctgtttt aggggttgtc ttaccggggg caagcgcagc tattaaatca acgacaaaag
    39901 gccaggttgg ggtcatcgga accccaatga cagtggcttc agacatttat cgcaaaaaaa
    39961 tccagctatt agcaccatct gttcaagtaa ggagtcttgc ttgcccgaag tttgtaccga
    40021 ttgtggaatc aaatgagatg tgttcgagta tagctaaaaa aatagtttat gacagtctat
    40081 caccattagt cggtaaaata gatacccttg tactaggatg tactcactat cccttgttac
    40141 gaccaattat ccaaaatgtt atggggccat ctgttaagct gattgacagt ggagcagaat
    40201 gcgtccgaga tatctctgtc ttattaaact attttgacat taacggtaat tatcatcaaa
    40261 aagctgtcaa acaccgcttt tttacaacag caaatcctga gatttttcaa gaaattgcct
    40321 cgatttggct gaaacaaaaa ataaatgtgg agcatgtcac cttatgagtg aaaaaattta
    40381 cgaatacaaa gatgaaaata actggtttat cggaaagatg actggtcata atttaatatc
    40441 gggctgggga gtcaaacaca cgacaatcaa aaaaatagat gaccttttag acggtattgc
    40501 cgcaacgtta gattgggaaa atcctaaagg ttacgatgtt tcggtggtgc gttatcagtc
    40561 tcctttatct ctcatcacct ttattatcga catgataaat caagagaccc agcgtaagat
    40621 taaggtaact ccccatgcag gcacgatttt attaatggaa aatgcgaaac tgcttgcagt
    40681 ttatctccca gaaggtggag tttcaacggc tacatttttt gcgactagtg aacagggatt
    40741 tggggatatt attttaatcg ctacacgaaa cgagggaaaa accaaagagt tccgcaatct
    40801 gtttggccag ctaggttatc gtgtggaaaa tctcaatgat tatccagaat tgccagaagt
    40861 agcagaaact ggtactactt ttgaagaaaa tgctcgcttg aaagcagaaa cgatttcacg
    40921 cctcacagga aaaatggtat tagcagacga ttcaggacta aaagttgatg ccttgggtgg
    40981 tttacctggg gtttggtcag ctcgtttctc aggaccagat gccacagatg ccaagaataa
    41041 tgccaaactc ttacacgaat tagctatggt gtttgaccaa aagaagcgct cagctcaatt
    41101 tcatacgact ttggtagtgg ctgctcccaa taaagatagt ttggttgtgg aagccgagtg
    41161 gccaggctat attgctacgc agcctaaggg agaaaatggt tttgggtatg accctgtctt
    41221 tatcgtcgga gagactgggc gtcatgctgc agaattagag gctgaccaaa agaatcagtt
    41281 gtcgcataga ggtcaagcag ttcgaaaatt aatggaggtc tttccagcat ggcaagcaaa
    41341 acaatcatag taatgagtga ctctcatggt gaccgagata tcgttcaagc cattaaagac
    41401 aagtatcttg gtcaagttga tgctattttc cataatgggg attctgaatt aaacagctca
    41461 gatcctattt gggctggtat ttatgtcgtt ggcggtaatt gtgactatga tacaggttac
    41521 cctgatcgtt tagtaactca attaggcaca gttacgattg cccagacaca tggtcacttg
    41581 taccatatta atttcacttg ggataaactg gactactttg ctcaagaagt agcagcagat
    41641 atttgtctat atggacacct acaccgtcca gctgcttggc aagttgggca aaccctcttt
    41701 atgaatcccg gatcagtaac acaacctcgc ggagaaatta acgagaaatt atatgctcgt
    41761 gttgaactaa ctgatactca aattaaagtt gattacttta caagagatca taaactttac
    41821 ccatcactgt ctaaggaatt taaacgatga tcgcaaaaga atttgaaacg tttttaatga
    41881 gtcatttaga taattatctt atcccggaac aagacttggc tatttttatc gacacacata
    41941 atgcagacca tgtgatgtta cttttggtga gcaatggttt ttctagagtt cctgttatca
    42001 cacgggagaa aaaatatgtg ggaaccatta gcatctcaga tattatgatg taccaatcta
    42061 aaaggcagct gacagattgg gaaatgagtc aaacagatat tggcgagatg gttaatacca
    42121 aaattgaaac cattagcatt acttccagcc taacagaaat tatgcacaag ttaattgatt
    42181 tcccattctt accggttgta gatagagcga atcgctttgt tgggattatt acgcgaaaat
    42241 ctatattaaa ggctgtcaac agtcttttac atgattttac agatgattac acgattataa
    42301 aaaaatgaag agttatattg aaccttttat tgctagcaag gctttatctc aaaatagtca
    42361 aaaagcttat cgatacgatt tgcaacagtt ttgtcaactg attggtgaac gtgtcaatca
    42421 agacaaacta ttactttatc aaaatagtat agctaacctc agtttatctg caaaaaaacg
    42481 caaattatca acagctaatc aattcttata ctacttgtat caaataaagt atttaaatag
    42541 ctattttcga ttaacagaca ccatgaaagt gatgcggact gagaagcagc aagctgctat
    42601 tattaatact gatattttct atcaaaaaac cccttttgtg tgggggcaat taatcagctt
    42661 attaatattg gagctgggtt taactccgag tgaagtagct ggtattgaag tagctaactt
    42721 ggatttaaat tttcaaatgt tgacgctaaa gactaaaaaa ggtgtgcgtg tattaccttt
    42781 gtcgcaaata ctaatccctt ttttagaaca acaactcgtt ggcaaagaag cctatctttt
    42841 tgaacatcga ggcatccctt tttcaagaca atggtttttt aatcacctaa aaacctttgt
    42901 tcgaagtatt ggttatgaag gattaactgc tcaaaaatta cgagagcaat ttattttgaa
    42961 agaaaaacta gctggtaaat cgataataga acttagcgat attcttgggc tgaaaagccc
    43021 tgtgacctta gaaaaatact acaaatcata atggatataa aacttaaaga ttttgaaggt
    43081 cctcttgatt tactcttgca cttggtatca cagtacaaag ttgacatcta cgaggttcct
    43141 attgttgagg ttattgagca gtaccttaat tatattgaaa ccttacaggt aatgaaactt
    43201 gaagtggctg gtgactacat gctgatggca agtcagctca tgttaatcaa aagtagaagg
    43261 ctattaccaa aggtggtaga acacatcgaa gaagaagatt tagaacaaga tctgttagaa
    43321 aagatcgaag aatatagtcg ctttaaagca gttagtcaag cgttagcgaa gcaacatgac
    43381 caaagggcaa aatggtattc aaaacctaaa caagaattaa tttttgaaga tgctatctta
    43441 caagaagata aaacggtgat ggatctattt ctagcttttt caaacataat ggcagctaaa
    43501 agagcagttt taaaaaccaa ccatacagtc atcgagcgtg atgattataa gattgaagac
    43561 atgatggctt ctatcaaaca gcgactggaa aaagaaaacg ttattagctt atcagcgatt
    43621 tttgaggaat gccaaacatt aaatgaagtt atcagtatat ttttagctag tctagaattg
    43681 attaagcttc acgtggtatt tgtagagcag ctctctaatt ttggggctat catactacga
    43741 aaggagaaga aatgacgtat ttgtcacaga tagaagcgct gctatttgtt gcaggtgaag
    43801 aagggttaag tttgcgacat ctagcgtcta tgttatcatt aacgccaaca gctcttcaac
    43861 agcaattaga aaaactctct caaaagtatg aaaaagatca acattctagt ctttgtttga
    43921 tagaaacagc taatacctac aggctagtaa ctaaagaagg ttttgcagaa ctcttgcgtg
    43981 cttatgccaa aacaccaatg aaccaaagtc tatctcgtgc gagtttagag gtcttgtcta
    44041 ttgttgcata caaacaacct attacacgaa tagaaattga tgacattcga ggggtaaact
    44101 ccagtggagc tttgagtaaa ttattggcat ttgatctcat tagagaagct ggcaaaaaag
    44161 atgtggttgg gcgtcctcat ttgtatgcga caacagatta ttttctggac tatatgggaa
    44221 ttaatcattt agacgaattg attgaagtat ctgccgttga accagcagat gaagagattg
    44281 ctctttttag gacacaagac tgagttttgc ttgtcatcaa atagcaaatg gcgtttacaa
    44341 aaggatttgt tttaaggcgt tttagttgaa aaaaagcctt aaactaggga caacaaaaag
    44401 ttgctcaaaa gaaagaagaa tataatgcga attaacaaat acattgccca tgcgggtatt
    44461 gccagtcgac gtaaggctga agaattgatt aagcaaggct tagtaacctt aaatggtcag
    44521 gttattacgg atttagcaac cactgtaaaa tcgggagacg tggttgaaat agaaggaagt
    44581 cctatttaca acgaggaaaa ggtttactat ttgcttaata aacctcgtgg cgctatctca
    44641 agtgtgtcag atgacaaagg ccgtaaaacg gtactggatt tactgcctca ggttaaagaa
    44701 cgcatatacc ctgttggacg attggattgg gacacatcag gggtccttat tttaacgaat
    44761 gatggtgatt ttacagatac tatgattcat cctagaaatg aaatagacaa ggtttatctg
    44821 gcgcgtgtca aaggaattgc aacgaaagaa aatcttcgtc ccttaactcg aggaattgtt
    44881 attgatggta aaaagaccaa accagctcgc tacaatattg ttcgtgtaga agctgataag
    44941 aatcgatcga tcgtagaatt gactatccat gaagggcgaa accatcaggt taaaaaaatg
    45001 tttgagtctg tggggctttt ggttgataaa ttatctcgta cacgttttgg gaccgtcgac
    45061 ttaaagggac taagaccagg agaagcaaga cgcttaaata aaaaagaaat aagtcaactg
    45121 cataacttag ctaatacgaa aaaatgatga aaaaactatt aatagtatct gttaaagctt
    45181 atcaaaaata catttctcct ttatcgcctc ctagttgtcg ctacaagcca acctgttcag
    45241 cttatatgtt aactgctatt gaaaaacatg gtactaaggg aatactaatg gggatagcaa
    45301 gaatattaag atgtcaccct tttgttgctg gtggtgtcga tcctgttcca gaagatttta
    45361 gcctaatgag aaataaaaac acgtctaaaa acgctgaaaa ggcatgactt tatcaaaaaa
    45421 tggcttgaca ttgatagctg aagtgttata atggaaaaca agcattcgtt ggtttaaatc
    45481 aaacctgtta tgatttaagt tagcgagtca ccatcaacca cattgtttgc tgagcatgac
    45541 tccgagcagt gtggtttttt aaattaaaaa gagtaaatga gattatgacg acaaaagaac
    45601 ttatcaacaa aaacgacaaa gtaaaaaagg ctcgcaatca tattgtcctg tttcaacccc
    45661 aaattccaca aaatactggt aatattgcta gaacatgtgc tgcaactaat gcacctttac
    45721 acattattaa acccatggga tttcctattg atgaccgcaa aatgaagcgt gccggcttgg
    45781 attactggga caagttagaa ctgcattttt atgatcattt agaacagttt atcaatcaat
    45841 gtcacggaca gcttcacctg attagcaagt ttgcagttaa taattattct caagctactt
    45901 atgctgatgg cgattcccat tatttcttgt ttggacgaga agatacaggc ctgccagaag
    45961 attttatgcg agaacatgct gaaaaagcac ttcgtattcc gatgaatgat gagcatgtac
    46021 ggagtttaaa tgtctcaaat acggtttgta tggtgattta tgaagccctt agacaacagg
    46081 gctttcaagg gttagagtta aagcatactt acgaacacga taaactaaaa taatggactt
    46141 tttaaaagcg tcagtttttg gcaacatgcg ttgtcagtag ctgacgcttt taaaaattta
    46201 gtcaaagcta ttgctatcta ggtttaaatc tgctatgcta gaagtgtctt cagggcaggg
    46261 tgtgattccc gaccggcggt gactaggtaa ctagaagtcc gcgagcgcaa gctgatgtgg
    46321 tgtaactcca caaccgacag tatagtctgg atgagagaag accgggcttt ttgtgtaggc
    46381 ttattttagc tacacaataa cgacggaact tctttcaatt tgaaaaaatt ggaggaattt
    46441 tttaatgtca aaaacacata aaatgattat gattggtata ctttctgcga tatcatttct
    46501 attgatgcta gttagctttg ccattattcc aggcgcagct tttttaaaaa ttgaatttag
    46561 tattattcct gtcttatttg gtttaatgat tatggactta aagagtgctt acttgatttt
    46621 gttattgagg tccttattga aactcttttt gaacaatcgt ggggttaatg attttattgg
    46681 tctcccaatg aatattatcg ctatcgcttt atttgtgacg gcttttgcct tagtttggaa
    46741 ccgtcaaaaa acgcttagtc aatatgtatt tgctagctta ctaggcacag ggttattaac
    46801 gtttggcatg gttgttctca attatacttt tgccattcct ttatatgcta tatttgcaaa
    46861 tattgatatc agagcttata ttggtgttac taagtatatg atgactatgg ttattccgtt
    46921 taatcttgtt gaggggttga tatttgcaat taccttttat tttgtgtata ttgcaagtaa
    46981 accaatttta gaaagatact tacactaata tgatgacaaa caaacaaact cactttttaa
    47041 tagcttcttt tgcattgcta atctttgtta taataggcta tacggtcaaa ttttttcctg
    47101 aacgtttggc acttctagat aatactattc aagcagagat tagaggcaac cttcctatag
    47161 tgctgacgca attctttagg ggtgttactg tatttggaaa tgtcatgaca caagttttgt
    47221 tagttattgt gtcggtccta gttttattct ttatgaagtg gaaaattgaa gccttgttta
    47281 tcttgtcaaa tggtgccatt gcagcatttt tgattacgac tctcaagctc ttttaccaaa
    47341 gaccaagacc tgctattgag catttggttt atgctggtgg ctattcgttt cccagtggcc
    47401 atgcgatggg tagcatgctg atttttggta gcctattgat tatttgttat cagaggctac
    47461 attcaaaact attacaattt gtgactagca tgatctttat aatactaatt ttattaattg
    47521 ggttatctcg tatttatcta ggagtacact acccaagtga tattttagca ggctttgtat
    47581 taggatttgg tattttgcac tttatttatc ctttttataa gcaaaaacga tttgaatggc
    47641 gatttctttt gaaacaagac taatttttct tgagaagaca taggcttatt taaatcattt
    47701 agaataacat tggcaagcat cacccccttg attgccagtg ttattttagt tttagttaga
    47761 tgatgtgagc tgatgattag taaatatgtt tagataaaaa acgattaaag tgctatgtaa
    47821 cgtcagaaaa gacgtgattt ttgttgatct gtaatcatta agtaagctga tgtagaagta
    47881 gtgaattcta atgactgagc tatcttttgc atagatgact atcttgctag taatgatttg
    47941 tgctacaata gaaacatgaa aaaaagatat cagacattaa atgagcatta ccgtcaacta
    48001 tttggcgcaa aaatgtttaa agttccgatt gatgccggct ttgattgtcc caatcgtgat
    48061 ggaacggtag ctcatggagg atgtactttt tgcacagtat caggttctgg agatgctatt
    48121 gtagcaccag atgctccaat taaagagcaa ttttataaag aaattgattt tatgcaccgt
    48181 aaatggcctg atgtcaatcg ctatttggtt tattttcaaa actttaccaa tactcatgat
    48241 acagttgatg tgattcgcga ccgttacgaa caagctatta acgaacctgg agttgttggt
    48301 attaatattg gcacacggcc tgactgtcta ccagatgata ccattgctta cttagcagaa
    48361 ttatcagaac gcatgcatgt gacagtggag ctaggcttac agacaactta tgaagaaact
    48421 tctaggctaa ttaacagagc acattcttat gatttgtata aagaaacggt gaggcgacta
    48481 aggcattacc ccaacattaa tattgtctcg cacttgatta atggattgcc aaaagaaact
    48541 catgacatga tgcttgaaaa tgtgagacgc tgtgtaacag acaatgacat tcaaggaatt
    48601 aaattgcatt tgctgcattt gatgaccaat actagaatgc aaagagatta tcatgaagga
    48661 cgcttaaagt tgttgagtca aaaggactat gtttcgatta tttgtgacca gctagagatt
    48721 atccctaagc atattgtgat tcaccgtatt acaggagatg ccccacgcga tatgttgatt
    48781 gggcccatgt ggagtctaaa taagtgggaa gtattgaatg ccattgataa agaaatggaa
    48841 agacgaggaa gctttcaggg ctgtaaagtt gactaatttg tctcattttg acggatttgg
    48901 ataggagaaa agtgatgcta aaacgcccca ttcatctctc gcatgatttt ttagcggagg
    48961 ttgtggataa gtctagtgtg gttgttgatg ccacgatggg taatggaaat gatactgcct
    49021 ttttggcaca attagctaaa aaggtttatg cttttgatgt gcaagaacaa gctattagaa
    49081 aaacaagtga gcggttagcg cagctaggat tatcgaacgc tgagctgatt ttagcgggtc
    49141 atgaagcggt tgatcagtat gtgacagagc cggtgcgcgc agcgattttt aaccttggtt
    49201 atttgcccag cgcagataaa tctattatta ccttaccaaa taccacactc caagctttat
    49261 caaaactact aacgttatta atggtaggtg ggcgtattgc tattatggtc tattatgggc
    49321 atgatggagg cagtttggaa aaagatgctt tattagattt tgtcaagcaa ttagaccaaa
    49381 gaaaggtttc tgcaatgctt tatcagcctc ttaatcaggt gaatacccca ccttttttaa
    49441 tcatgttgga gaaattagca gacgtttagc tcttatccct gtgtcaagtc tactagaagt
    49501 tctgcgatta tttcggtgga aaaagaagaa atcccatagg ctttggctat aaatgtgata
    49561 taatgaagtc actatattat ttttttagga gttacattaa catggaagac cctgtgagtc
    49621 agcccttagt gattcaattt ttattgttag ttgttttaac cttgttaaat gctttttttt
    49681 cagccagtga aatggcctta gtttctctca atcgttctcg ggtggaacaa aaagcagcag
    49741 acggtgataa aaaatacgct cgtttgttgc gggttttaga ggaacctaat cattttttat
    49801 caacgattca agttgggatt acctttatta gtttactatc aggagcaagt ttatcagctt
    49861 ctttgggtaa ggtgatctca ggttggctag gtaattcagc gaccgcaagg acagctggta
    49921 ctatcatctc cttggttttc ttgacttatg tctctattgt tttaggagaa ttgtatccaa
    49981 aacggattgc catgaacctc aaagacaagt tggcgattgt ttcagcccct attatcattg
    50041 gattagggag actggttagt ccctttgtat ggctcttatc agcttctact aatttactga
    50101 gccgacttac ccctatgacc tttgatgatg cagatgagca aatgacacgt gatgaaatcg
    50161 agtatatgtt atcaaaaagt gaggcgaccc ttgatgctga agaaattgag atgttgcaag
    50221 gagttttctc acttgatgaa atgatggcgc gtgaagtcat ggtcccaagg accgatgctt
    50281 tcatgattga cattaacgat gatccgcttg aaaatattca ggaaatctta aaacaaagtt
    50341 tttcacgcat tcctgtttat gatgtggata aagataaaat tatcggtctc atccacacta
    50401 agcgtctctt ggagtcaggt ttccgccagg gatttgatca gattaacatg cgaaaaatgt
    50461 tacaagaacc tctttttgtt cccgaaacca tttttgtaga tgatctctta cgccagctgc
    50521 gcaataccca aaatcagatg gctattttgc tagatgaata tggtggtgtg gcaggacttg
    50581 tgactttgga agacttgctt gaagaaatcg tcggtgaaat cgatgatgaa accgataaag
    50641 cagaacaatt tgttcatgag attggagaca atacctatat tgttgttggt actatgactt
    50701 taaatgagtt taatgactat tttgataccg aactagaatc agatgatgta gataccattg
    50761 ctggttttta tttgacaggt atcggaacca ttccaagcca ggagcaaaaa gaagcctacg
    50821 aaatagataa caaagacaaa catttagttc taatcaacga taaagtcaaa gatggccgta
    50881 ttacgaaatt aaaattaatc ctgtctaata tagaacagat tattgaggaa gactaggctc
    50941 catgcctagt ctttttgatg aaaagtgtta taatgaaaat gtaaaaacgt ttacaagtaa
    51001 tgaaggagat gcaatgactg agaaagatta tggacaagta acagggatgg tgcattcgac
    51061 agaaagtttt ggttctgtag atggtccagg cattcgtttt atcattttct tgcaaggctg
    51121 taaattacgt tgccaatatt gccataatcc ggatacttgg gagatggaaa ccaacaattc
    51181 aaaaatcaga acagtcaatg atgtcttgaa agaagccctt cagtacaaac atttctgggg
    51241 aaaaaaagga ggaattaccg tatctggtgg tgaagctatg cttcaaattg attttatcac
    51301 tgccttattc atcgaagcta aaaaattagg tatccatacc acactagaca cctgtggctt
    51361 tacctataga ccgacacctg aataccatca ggttcttgat aacttattgg cagtgaccga
    51421 tttgatttta ttggacttaa aagaaattga tgaaaaacaa cataaaattg tcacgcgtca
    51481 gcctaataaa aacatattgc aatttgctag gtatttatcc gacaagcaga ttcctgtctg
    51541 gattcgacat gtcttggttc caggcttgac tgatattgat gatcatttga cacgattggg
    51601 tgagtttgtc aaaaccttga aaaatgtcga taaatttgaa gttttgcctt accataccat
    51661 gggagaattc aagtggcgtg agctaggtat tccttatcag ttagaaggtg ttaaaccacc
    51721 aactaaggaa cgcgttcaaa atgctaaaaa tctgatgcag accgaatctt acacagaata
    51781 tatgaatcgg attcatcagt cataagagct gttatagggt tgaggtttcc tcaacttttt
    51841 aaagtattat aagcagatct tttgtaatct tcctttgatt ttagggggta attttgctaa
    51901 aataaaatga ataaacaaaa tgaggtagaa tgattatgtc aaaaatttta gtttttggtc
    51961 atcaaaatcc tgatacggat gctattgcat catcttatgc ttttgattac ttatcccaaa
    52021 aagcgtttgg tttggatact gaagtcgtag ctttaggaac acctaacgaa gaaacagctt
    52081 ttgcccttga ttattttggt gttgaggcac cacgtgtggt agagtctgct aaagcacaag
    52141 ggtctgaaca agtgattttg acagaccaca atgaattcca acaatccatt gcagatatcc
    52201 gtgaggttga ggtatatggt gtggttgatc accaccgtgt ggctaatttt gaaactgcaa
    52261 atcctttata catgcgtgtg gaaccagtag gatcagcctc atctattgtt taccgtatgt
    52321 ttaaagaaaa tggcatcgag gtgccaaaag cgattgcagg gatgctcttg tctggtttga
    52381 tttcagatac cttgttgctg aaatctccaa caactcatgt gtctgatcac cttgtcgctg
    52441 aagaattggc agagctagca gaagtgaact tggaagacta tggaatggct cttttaaaag
    52501 caggtacaaa ccttgcaagc aaatcagaag tagaattgat tggtattgat gccaaaacat
    52561 ttgaattaaa tggcaatgct gtgcgcgtgg cacaagtgaa cactgttgac attgcagaag
    52621 tcttagaacg tcaagaagct attgaagcag ccattaaaga tgccatggct gcagaaggtt
    52681 attcagattt tgttttgatg atcacggata ttgttaactc aaactctgaa attctagcta
    52741 ttggagcaaa catggacaag gttgaagcag cctttaactt cacccttgac aacaatcatg
    52801 ccttcttagc aggtgccgtt tctcgtaaaa aacaagtggt tccacaattg acagaaagtt
    52861 ttggagctta atgaaaagac gctttaaaag gacttgattt ttttgaccaa gtccttttgt
    52921 cactcttcta tgttataata gaaggtatga taacagtatt tcattcagat aaactcacca
    52981 gacaaccttt ctttcaagac cttatcaatt atctcgacca gcatgatcac gttatattac
    53041 gagagatcaa aaaagccttt cctaatgtga caggtattga caaggccatc gaaagctatg
    53101 ttcaagctgg ctatattcgc cgtgaaaata agcgttatgg aatcaatctt cctttggtga
    53161 gttctgatca gcagctggcc ttagacacta tgctttttgt ggacacctgt tcagctatgt
    53221 atgaaaatat tttagcggtt gtttttgaga ctcagctaac aaaccaaacc aatcacgtga
    53281 tgatcaagga aaagaccaac atcacgagag acgatttgac cctggctaat tatttttacc
    53341 gtctcaaaag aggtgagaag ccatcagctg agcagctgga cttgtatgac ctcttggggg
    53401 atgtgaatca ggaatatgcc cttaaatata tgacaacttt tttgcttaaa ttcacgcgca
    53461 aagaccttgt gatgcaaaaa cgtcctgata tatttgtgga agctctggtt acacttggtt
    53521 acctaaaaca agtggagcct acaacatacc agctgctaat gaccttggat aaagaaagtt
    53581 taacctttat agcaccatag aaaaaagcta ggaacaagga ctgttcttag ctttttgagt
    53641 gcttttattt tgtttttgcc attaagtaaa gaaaataagg ggctccgata agcgacacta
    53701 aaatccctgt ctggatatta gtgccaacca gatacactcg cccgactgta tctgcaacaa
    53761 gaagtaggat cattccgatt aaaatactaa tgggtattgt tattttatga ttactcccta
    53821 ccaagtatgt actaaagtgt ccagctatta agccgataaa actaatatta ccaaccaaaa
    53881 gaacactcaa agcacctaag ccagctgcta agacaagtat caaacgacgc ttacggttta
    53941 aagggagtcc tagcccaata gcagtattat cagctaatcc cataatatta aggaaatggg
    54001 cttgactata agtcaatagc caaaaacaca ataataaagg agcgatgaca ctcagagtag
    54061 gccagtcgtc tcctattaat tggccgctaa gccaattgat gaccaaatcg actttgtagc
    54121 gattaatatg accaacaaga gcaaccatta gacttgaaag catggtagta acagctacac
    54181 cagtaagaat taaccgtgta ggatcaattt gtccttgttt tgtcaaagat agccaataaa
    54241 cgctaaaggt tgttacaaca gcaccaagca tggcaaataa aggcaagtaa tggctgatat
    54301 gagcttgctc aaagaaagaa aaggagacca caatagccaa agagatacct gctcctatag
    54361 taatacctaa tacaccagag tcagctaagg gatttcgtgt taaacgttgt aataatagcc
    54421 ctgataaagc aagagaaccg cctcctagac aagcagctag aattcttggc agacgaatgt
    54481 taataacgat gaaagatatg gcatggctag attttcccaa aaacacatgg ataaggtcca
    54541 aaaaagatag atgagattct ccaagggaca aagctaatcc cataagactt acaagtagca
    54601 ggatcaggct agtatataac cccaaaaggt gtcggtgaga tttcaatagc ggcctcctct
    54661 tcttattaac cataaaaagc atggaaaacc aattaagcta accaatgccc caagaggggt
    54721 ttcataaggc ggattgagat tacgacaagc aatatcaacc cataccatga aactagcgcc
    54781 agaaactgca caaagcggca aaaggtatcg gtaatgatga ggtgtaaagt gtttcataag
    54841 atgaggaatg actaagccga taaaagaaat gcttccagca atggctacag ctgctgatga
    54901 taaaataaga accaaaatca taaagactgc actgatcaag ttggtctttt gcccaagagc
    54961 ttttgcctga gactcactta gactaaggac ggtcaggtga taagataata gctgggctaa
    55021 acaaagacta agaataataa gaggagcaat atagccgatc atttgccaat tgaccccgac
    55081 aagacctcct gcttgccagc cgataacagc atttgccaga tgatagtaat tggtaatacc
    55141 ttgacctaat gctgatagca gtatggaaac catagctcct gctaagacaa ggcggagctg
    55201 atggtagcct ttgccagatt gataagaaag gccaaagact aaggttgctg ccaggctaga
    55261 ccctaacaac gaaagcagaa taatgaggga atagtgcaag tgaggtacaa acgcataagc
    55321 cagtaccaag gctagtcctg ctccagcatt aatgcctaaa aggccaggct cagcgatagg
    55381 attacgtgtg attgcttgca tgatagttcc agaaacagct aacgcagagc cagttaaggt
    55441 agcaccaaaa agtctcggta atcgtatagc gagtaaaaca ttggctttat ggccgttttg
    55501 ctttccaaat aaaatagagt taagatcctg atgtgataaa gcaacggctc caaagcgcag
    55561 atgacagtaa atagcaacac ataagctaaa actaagtagc acaaagacaa gccaaaagat
    55621 atgtgactta gtgattatct gcgttttttt taagcaaaaa cgctcgagac acaaaaaggg
    55681 atgcttagtt ttcacttgat aagattgcct ctcgtaaagt ctctaattga tattctaaag
    55741 aaagaggatc agtaaagtaa aaaacattgg cattgacttt gatcacatgg tgttttttca
    55801 cagctggaat ggattgccat agcttacttt catatagagc agaaccagtc ttatcatctt
    55861 ctgcagcaac gacgacataa tcaccaatat agtctggcaa gacttcctgc gacaatgaca
    55921 ggtaaccttg tttaaaaact tcagtcttca ctttttcagg agcatcataa tggaaagctt
    55981 gatggataac ctctccgcca cgtccccagt ctttaccaaa aaggtaaacg tctttttcat
    56041 aaagccccat aatagtaaag gttgctttgt cacctgtaac ggctttgact tctttttcat
    56101 aagctgctgt tttagttttc caatctttta accatttctt ggctttgcct tctttgttaa
    56161 agatgcggcc gaagtcagat aacacttgta aataatcacg tttgcggtat tcaatcgaaa
    56221 taacaggcgc aatttctgcc aattgtttaa tattttcttc tgtagagcca acaacaatga
    56281 ggtcaggctt aagcgttgta atggcttcta aatcagttgc agcaacttgc tttgcttttt
    56341 ttacagtctt tgctaagatg gggtttttct tgtcataaga agtaaccccg actagattca
    56401 tatctaattt tttgaggtaa ccagtgtaag ttgaagccag actgactact cgcttaggct
    56461 gtttaggaat atctccgtaa taagtcacac cagcaatttg aggcataggc gataaacttt
    56521 ttgaggcggt gttagaatga ttagttgctt gattaccaca ggcgattaag gtaatagttg
    56581 tcaaacagag tgttaatagt agagttaatt ttttcatgta tgctaattct cctagaataa
    56641 agttattgaa gctgataagt cagcactata ggacagttgt ggataggatc atcaaccagt
    56701 accggtttga tttggaagat gtcttgaatg ataggtgaag tcataacatc tgctatagta
    56761 ccagtataat ggatcttgcc atgtttcata gcaatcagat gatccgaata gcgggctgaa
    56821 agattgaggt cgtgaagaac cataacaata gtcttgccgg catccttatt tagggattta
    56881 acgagctcta ggatttctag ttgatgattt aaatctaaat aagtagttgg ctcatctaaa
    56941 aaaatagtat cagtgccttg ggctaatgcc attgcaagcc aaaccctttg acgttgcccc
    57001 ccagaaagag catcaacagg ttgatcagca taggccatga cattggtggc ttgcatagcc
    57061 caatgaatgg cttttttgtc agctggacta agatttccaa agtaagattg atggggaaaa
    57121 cgtccgtaag aaaccaattc gtaaacagtt atgccgttag ttgcttcttg aacttgaggt
    57181 aaaagagcta gttttttagc aacctcttta gtttctaatg tggcaatgtt ttgcccattt
    57241 aaatatacaa ctccctgttt tggtggtaat aatcttgtca gtgcttttaa taaactagac
    57301 ttcccacagc cattggcgcc aataatggtc gtaattttac cttcaggaat ataaaacgat
    57361 aatttatcga tgatggtacg ctgttcatag gcaattgtga ggtcttcagc actaattgtt
    57421 gtcatatggt tgtctccttt ctaaaaagag tcaaagggtt gatgaaagtg cacattaaag
    57481 tattcatctg cccattttaa aatagttgag aagtagctta ttttctcaaa atcttcacta
    57541 ttatagcata gaaaaaaggc ttaacaaagc cttttttgat gtgattttta ggatgtatta
    57601 aaaaagaaat attagtttag aatgattaaa aacaatagcg ataatcattc taaactaacg
    57661 tcaacatact ctagaggtag tgtctggcga ttgctaaatc accagcataa gctacttgtt
    57721 gtcctttaac aatttgataa gcatctgcgc ctttcccagc gataatcaca gcatcttttg
    57781 ctccttggca aagagacatg gctttttgaa tagcttgctc acggtcacta ataatctcta
    57841 ctgggcgtgc gatatggctg gcaatttctt ttgaaatatc ttctggatcc tcgaaattag
    57901 ggtcatcagc agtgagaatg acggtgaggt ttggatgctg atgaatcacc ctaccaaaat
    57961 cagctcgtct gctctcgcct ttatttcctg gtgcacctaa aatcaacatt aatttgcctg
    58021 tctgatgttc ttccacaacg ctgagtaatt tttctaagct gtcaccatta tgggcataat
    58081 caacaaaaac tttagcatga tttgtcatag tgaggacttc catacggcct gggacgcgag
    58141 tcttagcgat acctttttga atatcagcta ggctagcacc caaacgaagg caggcaagtc
    58201 cagctgccat agcattttct tggttaaaat ggccaattaa ttgaatgtca taatggccag
    58261 ctaattgtcc tttagcttca aaggagaagg cttggctagt ggtgatctgg ttgtcagaca
    58321 agggaccata aaacacatgc tcttgatccg ctacctgatc cgctagaaat gaaaaatgat
    58381 ccatcccgct attgatcaca acagctctgc tattctccat taaaagacgc ttatgataga
    58441 aataatcttc aaacgttgga tgctcgattg ggccaatatg atcaggacta atatttagga
    58501 aaaccccaac atcgaacgta agcccataga cacggtcaac gaggtaagcc tgactagaaa
    58561 cttccataat caagtgggtc atgccattag tcacacactc agccatcata gcaaaaagat
    58621 ccaagctctc aggagttgtg agttgtgatt tgaaaaaggt cttgccatct aaggtggtat
    58681 tcatggtaga aaacatggca ggtttatagg attctttcaa catgtggtag gcaaaataag
    58741 cagccgtcgt ctttccctta gtacctgtaa aagctagtag tttgagcttt tcttgagggt
    58801 tgccatagaa aaccatggca attagactca tagctttttt gatatcagtg accaaaacaa
    58861 cgggaatacc aagctcgtaa tctacctctg aaatatagag ttgtaatccg ttagtgattg
    58921 cttctttaag gtagtcagct ttgaaagttg cccctttagc aaaaaaaagg gtcttgccat
    58981 ctacttgacg gctatcgtaa ctcaagcgct caaaactaaa gccttgataa tggtagtggt
    59041 atccgtcggc atcaagaacc tcacgaaaat tatggtcttt ttttaaaatg tctaataatt
    59101 gttcaatggt tatcataccc ttattttatc cttaaagttc ttgttttaca agtcacaagc
    59161 aaaagtttat aataaagcta atagaaacta ggaaagaata gatatgtcga cagaaaaaaa
    59221 acaactgaca caagaagaat tgatggttca gggagcagcc tggtccacag ctggcaattt
    59281 cattagccga cttttgggag tgttatacat tattccctgg tatatttgga tggggcaata
    59341 cgcgattcaa gccaacgctc ttttcaatat ggggtataat gtttatgctt attttttatt
    59401 gatttcaacc acaggactca atgttgcgat tgctaagcag gttgccaagt acaattccat
    59461 gggacaaact gagcatagct atcaattaat ccgaagtacc ctaaaactca tgcttggtct
    59521 gggccttatt ttttcagcca ttatgtatct gggctctcct ttatttgcga gcttatctgg
    59581 tggtgatgac acacttgttc ccattatgca cagcctttct ttagcagttt ttatttttcc
    59641 tgtaatgagt gtcattcgag gtatctttca aggccacaat aatatcaagc cttatgcgat
    59701 tagtcagatt gctgagcaac tcattcgggt tatctggatg cttttgacca ccttccttat
    59761 catgaaatta ggttctggtg attacgcttc agcagtaact cagtcaactt ttgcagcctt
    59821 tatcgggatg gtggctagta tgggtgttct ggggtactat ctttggaaac agggacttct
    59881 agcagctatt tttagtaagc cagatcatac tgtttccatt gatatcaagg gcttgttgct
    59941 tgaaacctta aaagaatcca tcccctttat cgtaacagga agtgccattc aagcctttca
    60001 attgattgat caatggactt ttgtcaatac tatgactctt tttacagatt atagccgttc
    60061 tcaattgctt gttttatttg gttactttaa cgcaaaccca gccaaaatta caatggtttt
    60121 aattgcagta gcagcatcta ttggtggtgt aggcattgcc ttattaactg aaaattatgt
    60181 caaaaaagac atgaaagcag ctgctcgctt aatcattaac aacattgaaa tgttagtaat
    60241 gtttttgtta cccgctctta ctggggcaat tattttagca agacctctat attctgtttt
    60301 ttacggagct agcgaggatc gtgccattca cctctttgtg gcggtcctct tccaaacctt
    60361 gttgatggcg ctttacaccc ttttttcacc gatgcttcaa gctctttttg aaaatcgaaa
    60421 agcgatttac tactttgcct atggtatctt gattaagtta gttttacaga taccgcttat
    60481 ttatttgcta catgcttatg gtcctttact agcgacgacg attgctttag tggtgccgat
    60541 ttatttgatg tatcgacgtc tatatcaggt tactcatttt aaccgcaaac tgttgcaaaa
    60601 acgtttatta ttaaccttga ttgaaacctt attaatggga ctggtcgtgt ttgtggccaa
    60661 ctggctattg ggctatgtct ttaaaccgac aggccgcttg accagccttc tttacctcct
    60721 cattattggt ggcttgggaa tgacggttta cacggcactc accttgctga cgcatcaact
    60781 ggataaatta attggtagca aagctagtcg ccttcgtcag aaattaggct ggcattaata
    60841 taatgacacc aagaacgatt tcgtgagtgc ttatcgaaat cgtttttttc ttaatgatct
    60901 cgcttagtaa aggtagccga taaaaaatca ggatttccca agcaaactac tgatattttt
    60961 ggtataatga taaaagtgta aaaaaataga aggaagtata tcaacatggg aaaatgtcaa
    61021 gttatttcgc atccgcttat ccaacacaaa ctttcaatct tacgtcgtca aaccacatca
    61081 actaaagatt ttcgtgagtt ggttaatgaa attgcgatgc tgatgggata tgaagtgtca
    61141 cgcgaccttc ccttagaaga tgtcgacatc caaacgccag tatcaaaaac ggtccaaaaa
    61201 cagttagctg gtaaaaaatt agctattgtt ccgattcttc gtgctggtat tgggatggtt
    61261 gatggcttgt taagtttggt accagctgca aaagttggcc atatcggcat gtaccgtaat
    61321 gaagaaactc ttgaacctgt tgaatacctc gttaaactac cagaagatat taaccaacga
    61381 caaattttcc ttgttgatcc aatgttggca acaggtggtt cggctatctt agcagtggat
    61441 tcgttgaaaa aacgtggtgc agcaaatatt aagtttgtct gtttagttgc agcaccagaa
    61501 ggggtgaaaa aacttcaaga agctcatcca gatattgata tcttcacagc agcacttgat
    61561 gatcatttaa atgaacatgg ctatatcgtg ccaggattgg gagatgctgg tgatcgttta
    61621 tttgggacaa aataaaagag acaaccatca aatagtagcc tttttaggcc aatcgttctt
    61681 gattttgtta aaaaaatcag agagatttct cttctttaaa ggaaaagcaa gtttatgatt
    61741 tgacctttat tgaccaaaat attataataa agacaatcag taaaaaagag atgacctagt
    61801 gggttgccgt ctctcaaaat gacgctacta aaaacccttg tagattggag aatgtattat
    61861 gattcctgtt gttattgaac aaactagccg tggagaacgt tcttatgaca tttattcacg
    61921 tttattaaaa gaccgtatta tcatgttaac agggcctgtc gaagacaaca tggccaactc
    61981 tgtcatagcg cagctcttat tccttgacgc gcaagacaac acgaaagata tctacctata
    62041 tgtcaacaca ccaggtggct ctgtctcagc aggcttggct attgtcgata ccatgaactt
    62101 tatcaaggcc gatgtgcaaa ctattgtcat gggtatggca gcttctatgg ggacagtcat
    62161 tgcttcatca ggaacaaaag gcaaacgctt tatgttacca aatgcagagt atatgatcca
    62221 ccagccaatg ggtggcacag gcggtggcac gcaacaaacg gatatggcta tcgcagcaga
    62281 acatctctta aaaacacgtc accgtttaga aaaaatctta gcacaaaatg ctgggaaaac
    62341 gattaaacaa attcataaag atgctgagcg tgattattgg atgagtgctg aagaaacctt
    62401 gacttacggt ttcattgatg aaatcatgga aaacaacgaa ctaaaataaa gaggatatct
    62461 gcaaaagtat tctagaaatt tctaggatgc tttttctctt agaaaggaaa ataatgatac
    62521 agcctactgc attaaaacag cttcaagagt taaaacgtca gttcagatcc ctttgcaacc
    62581 aattatgaga tagtttgagg atgatcctta gcagctgtgt gcaagaagtt atgacgattt
    62641 cataaaggat gacctagcca gtctttcttc gtctctttta cttaaagtgt ggtataatca
    62701 gattatgcta gccaaaatta aaaaaggcat tgccattaaa ggtgttgtag atcggcttat
    62761 cctagatctc ataacaatgc tgtagtaccg cccttacctt gctactaaag gcgctgttga
    62821 agcacaaatt gacttgcaaa ggatcttgac tgataggtat atgtctggtg gaatcgccaa
    62881 agcagcaaaa agaccaagac ccttatttgg tctctaccaa aatataatag aaaaggattt
    62941 agaatgtttc aaaaacaaga acgtattgga ctcgtcgttt acctgtatta taaccgagat
    63001 gccagaaagt tatcaaaatt tggtgattta tattaccatt ccaagcgctc tcgttatctc
    63061 attgtttata ttaataaaaa tgatttagac acaaaattag aagaaatgag acgtttgaaa
    63121 tgtgttaaag acattagacc atcagctttt gatgatattg accgccaatt tgtaggtaac
    63181 cttcatcgcg acgaaacaaa caaccatcaa aagggttaac aaccccctag ttactgatta
    63241 gcgttttaga tttagttagc ttattaaagt aagaaggttt tgacagatgc tcacactact
    63301 tcaagacaat gagacaacta gagcattagt gaataactga gttgacggtc acttctttaa
    63361 taagaacgga taggattggt cctaaaatag agcactgcaa agatatgcca gtgcttttta
    63421 gctcattttt tgataaaata gaactactaa aaagtgaagg aaataagatg atcacaggaa
    63481 aactcataac cgttgaaggt ccagatggcg cagggaaaac gactgtttta gagcaactca
    63541 ttccactgtt aaaacaaaaa gtggctcagg agatcttgac aacaagagag cctggaggtg
    63601 ttgccatttc agaatacatt agagaactta ttttagacat caaccatact actatggacc
    63661 ctaaaacaga actcttactt tatattgcgg ctcgaaggca gcatttggtg gaaaaggtac
    63721 taccagctct tgaagcaggc cagttggttt ttattgatcg tttcatcgat agttcagtag
    63781 cttatcaagg tgcaggacgc ggtttgatca aagcagacat tcagtggctc aacgagttcg
    63841 caacagatgg tttagagcct gacttgaccc tttattttga tgttccttct gaaattggtt
    63901 tagcacggat taacgcgaac caacaaagag aagtcaatcg tttagatttg gaaacaatag
    63961 agatacatca acgagtacga aaaggatacc tagccttagc taaggagcat ccaaaacgta
    64021 ttgtcacaat tgatgcgact aaaccattga aagaagttgt ctctgtcgct ttggaacatg
    64081 tcttagcctt gcttttagcc taggataagg gaggggagga atggatttag cgcaaaaagc
    64141 tcctaacgtt tatcaagctt ttcagacgat tttaaagaaa gaccgtctga atcatgctta
    64201 tcttttttcg ggtgattttg ctaatgaaga aatggctctt tttttagcta aggtcatctt
    64261 ttgtgagcag aaaaaggatc agacgccctg cgggcattgt cgctcttgtc aattgattga
    64321 acaaggagat tttgccgatg tgacggtatt ggaaccaaca gggcaagtga ttaaaacgga
    64381 tgtggtcaaa gaaatgatgg ccaacttttc tcagacagga tatgaaaaca aacgacaagt
    64441 ttttattatc aaagattgtg acaaaatgca tgtcaatgcc gctaatagct tgctaaagta
    64501 tattgaggag cctcagggag aagcttacat atttttattg accaatgatg ataacaaagt
    64561 gcttccgacc attaaaagtc ggacacaggt ttttcagttt cctaaaaacg aagcctatct
    64621 ttaccaattg gcacaagaaa agggattatt aaaccatcag gctaagctag tagccaaact
    64681 tgccacaaat accagtcatc tagaacgtct gttgcaaacg agcaagcttt tagaactgat
    64741 aactcaagca gagcgttttg tatctatttg gctgaaagat cagttgcagg catatttagc
    64801 gttgaaccgt ctggtacagt tagcaactga aaaagaagaa caagatttag ttttgaccct
    64861 tttgaccttg ctcttggcaa gagagcgtgc gcaaacgcct ttgacacaat tggaggctgt
    64921 ctatcaggct aggctcatgt ggcagagcaa tgttaatttt caaaacacat tagaatatat
    64981 ggtgatgtca gaataagtgc aattggaatt cgttatgggg aaacgaagct tgtcaaaaga
    65041 cttgcccgta ctcccttttc agcagaagag cacatggatt tttacaactg ttaaaagatt
    65101 tagcggccga ttttaggtca agaagtgagc tgccccagat taacagccaa gaagatttca
    65161 ttttctatgg aggctatgga ccgtgtggca ggccactgtg ctgcattcgt ttttgggaaa
    65221 atttctgaca gtatctatta aaatactaaa aaatcaaggg ttatcattga ctttgggtaa
    65281 agcaacaagc ccttatggcc gtttgctttg ctgtctctat gatgaaggtg gcttttacca
    65341 ataaagcaag aaaaaattcc ctgaatatgg taccatagta gagacaagtg atggccctgc
    65401 aacagtagca gccgttgata tatttacaga tcccattaag gtgcggttaa aagaccagtt
    65461 gaccttagtc acttatgctt tagaggaggt taaagtaggt gaataaaaaa gaattattcg
    65521 atgcttttga tggattttcc caaaatttaa tggttacttt agctgaaatt gaagccatga
    65581 aaaaacaagt ccaatcacta gtagaagaaa acacgattct tcgcctagaa aatacaaaac
    65641 tacgggaacg tttgagtcac ctagaacatg aaacggtagc taaaaatcct tccaaacaaa
    65701 ggaaggatca cttagaggga atttatgatg aagggtttca tatctgcaat tttttctatg
    65761 gacaacgtcg tgaaaatgat gaagaatgta tgttttgcag agaattatta gatagaaagt
    65821 aagggcatgc aggttcaaaa aagttttaaa gacaaaaaaa caagcggaac cttgtatttg
    65881 gttccaaccc caattggcaa tctgcaggac atgaccttta gagcagtggc aacgttaaaa
    65941 gaggttgatt ttatttgtgc agaagacaca agaaacacag gtcttttgct aaaacacttt
    66001 gacattgcta ccaagcaaat cagctttcat gagcacaacg cctatgagaa gattcctgat
    66061 ttaattgatt tattaatatc aggacggtca ctagctcagg tgtctgatgc aggaatgcca
    66121 tcgatttctg atcctgggca tgatttggtc aaggctgcta ttgatagtga tatcgcagtc
    66181 gttgctttac caggtgcctc agctgggatt acagctttga ttgctagtgg actggctcct
    66241 caaccccatg ttttttatgg gtttttaccc agaaaagcag ggcaacaaaa ggctttcttt
    66301 gaggacaaac accattaccc agaaacacaa atgttttatg aatcgcccta tcgcatcaaa
    66361 gataccctga ccaatatgtt ggcttgttat ggtgatcgtc aagtggtgct tgtcagagaa
    66421 ttaaccaagc tttttgagga gtaccaacgg gggtcgattt cagaaatctt gtcctacctt
    66481 gaggaaacac ccctcaaagg ggagtgtctg ctgattgtgg ctggagccca agtagacagt
    66541 gaggttgagc tgacagctga tgttgactta gtgtctctag tgcaaaaaga aattcaagca
    66601 ggagccaagc caaatcaggc tattaaaaca attgctaaag cttaccaggt aaatcgccaa
    66661 gagctttacc aacaatttca tgacttataa gtcatcgtag gttaggagaa aaacaaatga
    66721 cagaagatca agcaactatc cttattttag gagtatacgg aatcatattg gctgtattct
    66781 tattatcagc tttattatgc cttatcgcta actggcgact ctttaaaaga gcaggttaca
    66841 aaggatggta tagtctcatt cctttctatg caggttttgt caaacacaaa atcacttttg
    66901 gagaagataa taaatggttt tactttattg gttgggtcat tgctatttat tattactatg
    66961 tgcacttttg ctatgtccgt gcttttggtg gatcaaaggg tctagcagta tttggcttgt
    67021 ttttcccagg cattgcttct ttgatagtgg cttttggaaa aacttatacc acacaagaat
    67081 gccaccctgt gccacatctt ttaaactaag agaaaaaaga ccatcaattg gatttattga
    67141 tggtctttta ggtgtttaaa tgattttagt cccgtgaaca atggccgttc cagtgagttg
    67201 actcaagctt tggcaattct cagcagtgac gcctccccca atcatgattt caatgcgttt
    67261 attggcatag gtcacaagac ttttaagctg ttccacattg tctgtaatag gagtagcttc
    67321 aggtgagcca tgagttagca cacgcacaaa gccataatca atcagttggt caagcgcttg
    67381 gtgctggtga tcagtcggaa tccggtcaaa agccatgtgg aaaaccagtg gcaagccttg
    67441 tgtagcaggc agtaattgct cgatagcgtc ggtatccagt tgattttctg aggtcagcaa
    67501 gcctaagact agcgcatctg agccagcttc aacagctttt aagatatctt cttccatagc
    67561 ttttagttcg aggtcgttat agacaaaatc gccaccgcga ggtcgaatca tggtcgctac
    67621 gctaatcttt ttatcgtgta agagctgaca ggcttctttg atgacgccat aagatggcgt
    67681 ggtccctccg acagctaggt tatcacagag ttccacacga ctgacttgac cagcatcaag
    67741 ggttggcagt agtgtgaggt tctcagcaca aaattcttta atcattttta gttctccttt
    67801 atcactaata ttatagcacg aatgaagcta gtcatcagga aacatttttg tcaaatcatg
    67861 gacattatgc tatactcact agtattaaaa gtaggccaaa acagcagcaa atactttgaa
    67921 aaaagattgc tatgttctac ggcaatatca gtctttacca acgaactctc aagaagaaaa
    67981 gaaagacaaa gcttagcatt ttaaaaaagc cccccttttt ttgtgaaatg atgaccaaaa
    68041 acaggcgcct cacgcctaaa acagttaccc taaaggagaa caaatgatta cgatttacca
    68101 atacccaaaa tgtagcacct gccaaagagc catggctgaa ttaaagcaac tggttagtga
    68161 ctttgaagcc attgacatca aagctaatcc acccaaagct caggacctca aacattggat
    68221 ggaaacatct ggttatacca tcaaaaattt ctttaatacc agtggtaaca gctaccgcga
    68281 attgggactt aaagataaaa tagaccagct tagccttgat aaagctgctg aacttttagc
    68341 cacagatggt atgttgatta aacggcctat tctgattaag gacggtaacg tcttacaagt
    68401 tggctatcgc aagccatatc aagagttgga tttaggataa tgcgacaaag tctcaaaaaa
    68461 ggacaaggca acattataac gccttgtcct tttttgggat ctgtttttag acattagaga
    68521 tcaatatcta gtaaaattgg cgtatggtct tggcgttccc cagaagaaat catttcagaa
    68581 cgcttaacct tatccacgat tcggtttgaa gctagccaat agtcaattct ccaaccggta
    68641 ttattgattt tgctggtttt actacgttgt gcccaccagg tataaacatg aggaatatcg
    68701 ccgtgcaagt gacgaaaagt atctgtaaag cctcttgcta ataggttagt aaagccttga
    68761 cgttcttcgt cggtaaaacc aggagaacgt cgattgctgc ttggatttgc caaatcaatc
    68821 tctttatggg caacgttgta atctcccgta gccaaaacag gcttttgagc atccagttca
    68881 gtcaaataat cagcgtattt gtggtcccaa atttgacggt catctaaccg tcttagacca
    68941 tcaccagcat taggcgtata aacctgtgtg acgaaaaagt cttcaaattc tagggtaatg
    69001 atacggccct cagcatccat tgttgttggg gcaccaattt ctggaaaagt aatgacaggg
    69061 tttaacgtgt ttttgtacag gaacatggtg ccagcatagc ctttcctagc aggctcaact
    69121 gaagaacgcc acacatgcaa atagtttgga aagtaactaa gtagggtctc aatatgtttt
    69181 ttggtaggcc ccttagcaga taatttagtt tcttggatgg caataatatc cgcatcttga
    69241 gcgactaaag tgtctaaaac agcgcgtgat aaaagcgcac gaggggactc tcctgtgaga
    69301 gcagcattta aggaatcaat gttccatgaa atcagtttca tttggtttct ctttctaaca
    69361 gatagtcttg ttgttattgt aacaaaaaac gaaacgataa gcgactttgg gcacctagcc
    69421 cccataaaac agtctagcca aatgaagcat agcagcgtaa gaacaagaca aggaggtgat
    69481 cagtgagcat atcgtttgac agttaagagt aggattacta taatagaaac ataaatgtaa
    69541 gcgctaacaa aataaaaaat ataggagaat gtcaatggca caaaaaacag tcatcaccga
    69601 agaaacaacc gattttgtta tggatttcaa aacaagtagc gctgagggaa atgttgattt
    69661 tattaacgtt tttgaccttg agaaaatggc acaacaggtc atccctaaag gagcctttgg
    69721 ttatattgct agtggtgctg gtgatacctt taccctccat gaaaatattc gctcatttaa
    69781 tcataaatta attgtccctc actccctcaa aggggttgaa aatccgtcaa cagagattac
    69841 gtttgatggt gatcacttaa cctctcctct tattttagca cctgtagcgg ctcataagct
    69901 ggccaatgaa caaggagaag tagcgtctgc caaaggattg aaggaatttg gaagtatcta
    69961 caccactagt tcctactcaa caactgacct gccagaaatc agtgctgcct taggtgggac
    70021 accccattgg tttcaatttt attgcagtaa agacgatggc attaatcgta atattatgga
    70081 tcgtgtcaaa gcacaaggtt gcaaagctat cgttttgaca gcagatgcaa cagttggagg
    70141 aaaccgcgaa gttgataggc gaaacggctt tgtttttccg gtcggtatgc caattgttca
    70201 agaatattta cctgatggtg caggtaaaac aatggattat gtctataaat cagctaaaca
    70261 agcgcttacc tcaaaagata ttgaatatat cgcaacctac tcaggcctgc ctgtttatgt
    70321 caaaggacca cagtgtgctg aggatacctt gcgggcattg gacgcaggag catcaggtat
    70381 ctgggtaacc aatcatggtg gccgtcaatt agatggtggt ccagctgctt ttgattcctt
    70441 acaagaagtc gctgaagcag ttgatcacaa agttcctatt gtctttgact caggtataag
    70501 acggggtcaa catattttca aagctcttgc ttcaggtgct gaccttgttg ccttggggcg
    70561 tccagcgatt tacggcttgg ctatgggagg aagcattggt actcgtcagg tctttgaaaa
    70621 gttaaatgac gagttaaaaa tggttatgca actggctggg acacaaacta tccaagatgt
    70681 taaagccttt aaccttcgcc acaatccgta tgattcatcg attccatttg atcaaaatgc
    70741 actacgctta gattagaaca gacaaaaacc tctgctatca tgtcacaaat agtagaggtt
    70801 ttacactgca aaaagaggta tgaaaatcca ataacatagt tacaaggctt tcgtttaata
    70861 ccccccacta atagattaaa atgaaagact tcttacatta atagaagttt ctgttgcttt
    70921 tctcagaaaa atgtggttaa ataatcaaat attattaacg aaaaatgagt taatgattaa
    70981 cattaaactg gttttagtta aaacatttag gagggtatca ggtggagaaa aagcaacgtt
    71041 tttcccttag aaaatacaaa tcaggaacgt tttcggtctt aataggaagc gttttcttga
    71101 tgatgacaac aacagtagca gcagatgagc taaccacaac gagtgaacca acaatcacga
    71161 atcacgctca acaacaagcg caacatctca ccaatacaga gttgagctca gctgaatcac
    71221 aatccccaga cacatcacaa atcactccca agataaatcg tgaaaaagag caaccacaag
    71281 gtctagtctc tgagccaacc acaactgagc tagctgacac agatgcagca ccaatggcta
    71341 atacaggtcc tgatgcgact caaaaaagcg cttctttacc gccagtcaat acagatgttc
    71401 acgattgggt aaaaaccaaa ggagcttggg acaaggggta caaaggacaa ggtaaggttg
    71461 tcgcagttat tgacacaggg atcgatccgg cccatcaaag catgcgcatc agtgatgtat
    71521 caactgctaa agtaaaatca aaagaagaca tgctagcacg ccaaaaagcc gccggtatta
    71581 attatgggag ttggataaat gataaagttg tttttgcaca taattatgtg gaaaatagcg
    71641 ataatatcaa agaaaatcaa ttcgaggatt ttgatgagga ctgggaaaac tttgagtttg
    71701 atgcagagcc aaaagccatc aaaaaacaca agatctatcg tccccaatca acccaggcac
    71761 cgaaagaaac tgttatcaaa acagaagaaa cagatggttc acatgatatt gactggacac
    71821 aaacagacga tgacaccaaa tacgagtcac acggtatgca tgtgacaggt attgtagccg
    71881 gtaatagtaa agaagccgct gctactggag aacgcttttt aggaattgca ccagagaccc
    71941 aagtcatgtt catgcgtgtt tttgccaacg acgtcatggg atcagctgaa tcactcttta
    72001 tcaaagctat cgaagatgcc gtggccttag gagcagatgt gatcaacctg agtcttggga
    72061 ccgctaatgg tgcacagctt agtggcagca agcctctaat ggaagcaatt gaaaaagcta
    72121 aaaaagccgg tgtatcagtt gttgtagcag caggaaatga gcgcgtctat ggatctgacc
    72181 atgatgatcc attggcaaca aatccagact atggtttggt cggttctccc tcaacaggtc
    72241 gaacaccaac atcagtggca gctataaaca gtaagtgggt gattcaacgt ctaatgacgg
    72301 tcaaagaatt agaaaaccgt gccgatttaa accatggtaa agccatctat tcagagtctg
    72361 tcgactttaa agacataaaa gatagcctag gttatgataa atcgcatcaa tttgcttatg
    72421 tcaaagagtc aactgatgcg ggttataacg cacaagacgt taaaggtaaa attgctttaa
    72481 ttgaacgtga tcccaataaa acctatgacg aaatgattgc tttggctaag aaacatggag
    72541 ccctgggagt acttattttt aataacaagc ctggtcaatc aaaccgctca atgcgcctaa
    72601 cagctaatgg gatggggata ccatctgctt tcatatcgca cgaatttggt aaggccatgt
    72661 cccaattaaa tggcaatggt acaggaagtt tagagtttga cagtgtggtc tcaaaagcac
    72721 cgagtcaaaa aggcaatgaa atgaatcatt tttcaaattg gggcctaact tctgatggct
    72781 atttaaaacc tgacattact gcaccagggg gtgatatcta ctctacctat aacgataacc
    72841 actatggtag ccaaacagga acaagtatgg cctctcctca gattgctggc gccagccttt
    72901 tggtcaaaca atacctagaa aagactcagc caaacttgcc aaaagaaaaa attgctgata
    72961 tcgttaagaa cctattgatg agcaatgctc aaattcatgt taatccagag acaaaaacga
    73021 ccacctcacc gcgtcagcaa ggggcaggat tacttaatat tgacggagct gtcactagcg
    73081 gcctttatgt gacaggaaaa gacaactatg gcagtatatc attaggcaac atcacagata
    73141 cgatgacgtt tgatgtgact gttcacaacc taagcaataa agccaaaaca ttacgttatg
    73201 acacagaatt gctaacagat catgtagacc cacaaaaggg ccgcttcact ttgacttctc
    73261 gctccttaaa aacgtaccaa ggaggagaag ttacagtccc agccaatgga aaagtgactg
    73321 taagggttac catggatgtc tcacagttca caaaagagct aacaaaacag atgccaaatg
    73381 gttactatct agaaggtttt gtccgcttta gagatagtca agatgaccaa ctaaatagag
    73441 taaacattcc ttttgttggt tttaaagggc aatttgaaaa cttagcagtt gcagaagagt
    73501 ccatttacag gttaaaatct caaggcaaaa ctggttttta ctttgatgaa tcaggtccaa
    73561 aagacgatat ctatgtcggt aaacacttta caggactcgt cactcttggt tcagagacca
    73621 atgtgtcaac caaaacgatt tctgacaatg gtctacacac acttggcacc tttaaaaatg
    73681 cagatggcaa atttatctta gaaaaaaatg cccaaggaaa ccctgtctta gccatttctc
    73741 caaatggtga taacaaccaa gattttgcag ccttcaaagg tgttttcttg agaaaatatc
    73801 aaggcttaaa agcaagtgtc taccatgcta gtgacaagga acacaaaaat ccactgtggg
    73861 tcagcccaga aagctttaaa ggagataaaa actttaatag tgacattaga tttgcaaaat
    73921 cgacgaccct gttaggcaca gcgttttctg gaaaatcgtt aacaggagct gaattaccag
    73981 atgggtatta tcattatgtg gtgtcttatt acccagatgt ggttggtgcc aaacgtcaag
    74041 aaatgacatt tgacatgatt ttagaccgac aaaaaccggt actatcacaa gcaacatttg
    74101 atcctgaaac aaaccgattc aaaccagaac ccctaaaaga ccgtggatta gctggtgttc
    74161 gcaaagacag tgtcttttat ctagaaagaa aagacaacaa gccttataca gttacgataa
    74221 acgatagcta caaatatgtc tcagtagaag acaataaaac atttgtggag cgacaagctg
    74281 atggcagctt tatcttgccg cttgataaag caaaattagg ggatttctat tacatggtcg
    74341 aggattttgc agggaacgtg gccatcgcta agttaggaga tcacctacca caaacattag
    74401 gtaaaacacc aattaaactt aagcttacag acggtaatta tcagaccaaa gaaacgctta
    74461 aagataatct tgaaatgaca cagtctgaca caggcctagt cacaaatcaa gcccagctag
    74521 cagtggtgca ccgcaatcag ccgcaaagcc agctaacaaa gatgaatcag gatttcttta
    74581 tctcaccaaa cgaagatggg aataaagact tcgtggcctt taaaggcttg aaaaataacg
    74641 tgtataatga cttaacggtt aacgtatacg ctaaagatga ccaccaaaaa caaaccccta
    74701 tctggtctag tcaagcaggc gctagtgcat ccgctattga aagtacagcc tggtatggca
    74761 taacagcccg aggaagcaag gtgatgccag gtgattatca gtatgttgtg acctatcgtg
    74821 acgaacatgg taaagaacat caaaagcagt acaccatatc tgtgaatgac aaaaaaccaa
    74881 tgatcactca gggacgtttt gataccatta atggcgttga ccactttact cctgacaaga
    74941 caaaagccct tggctcatca ggcattgtcc gcgaagaagt cttttacttg gccaagaaaa
    75001 atggccgtaa atttgatgtg acagaaggta aagatggtat cacagttagt gacaataagg
    75061 tgtatatccc taaaaatcca gatggttctt acaccatttc aaaaagagat ggtgtcacac
    75121 tgtcagatta ttactacctt gtcgaagata gagctggtaa tgtatctttt gctaccttgc
    75181 gtgacctaaa agcggtcgga aaagacaaag cagtagtcaa ctttggatta gacttaccgg
    75241 tccctgaaga caaacaaata gtgaacttta cctaccttgt gcgggatgca gatggtaaac
    75301 cgattgaaaa cctagagtat tataataact caggtaacag tcttatcttg ccatatggca
    75361 aatacacggt cgaattgttg acctatgaca ccaatgcagc caaactagag tcagataaaa
    75421 tcgtttcctt taccttgtca gctgataaca acttccagca agttaccttt aagatgacga
    75481 tgttagcaac ctctcaaata actgcccact ttgatcatct tttgccagaa ggcagtcgcg
    75541 ttagccttaa aacagctcaa ggtcagctaa tcccgcttga acagtccttg tatgtgccta
    75601 aagcttatgg caaaaccgtt caagaaggca cttacgaagt tgttgtcagc ctgcctaaag
    75661 gctaccgtat cgaaggcaac acaaaggtga ataccctacc aaatgaagtg cacgaactat
    75721 cattacgcct tgtcaaagta ggagatgcct cagattcaac tggtgatcat aaggttatgt
    75781 caaaaaataa ttcacaggct ttgacagcct ctgccacacc aaccaagaca acgacctcag
    75841 caacagcaaa agccctacca tcagcgggtg aaaaaatggg tctcaagttg cgcatagtag
    75901 gtcttgtgtt actcggactt acttgcgtct ttagccgaaa aaaatcaacc aaagattgat
    75961 aacaaaacgc aaagagacaa caaatcatcc cttttaacag tcggaccttt ctttgagatt
    76021 gagctggcag atgatcattt cttaacaaaa gttaaagagt tgagcaaagt aaggaacaga
    76081 cataaagacc ataaaagcaa gccagtttgg cttgctttta tggtgttatt gtcgtgtgct
    76141 acgatgactg aaaaagaaca acgaacaatg acctcgaatc accaaaagga cttgatttga
    76201 tagcttccct atgctatact aagcctattc tttcaagaag gactcctttt gtggaaaaga
    76261 ctgaaaaaaa ggacactaat ctatccaaaa actccagatg agtagctgca aacggatgcc
    76321 tctgctgtga tcaaggggat aaagcagact ggcttgtcac tacctgagca ggtgtcaaaa
    76381 aaagaagctt ttggctttga cattcaacat actgagcttt agtattttag aacggagaca
    76441 cccccagttt tgtactcaaa aagcatgaac agttctgctg atgtgacatc tgatttctac
    76501 gtgcgcagtg agccaaaaca gctgctgttt gctatggaaa aagtggcgcc aatcgagatt
    76561 tttgagaccc taggctcaaa aataaggcat agaattccaa aggaagtcgc tgccgtgagc
    76621 acaacctaag aaaaactatt ccaaaagact ttttctgcaa tttgaaatac tgaatttcag
    76681 tattttttct tttgtaacta agagcaatga atgaggtaaa aaaaggcaac aatgtcaaaa
    76741 aagaaacgaa aacactatca ggtttatgat ggattacgct gtgctatgac gctttgtttt
    76801 atcagtggct atgtcaatgc ctttacctat atgacccaag gcaaacgttt tgcaggtgtt
    76861 cagacaggca accttttgtc atttgccatt cgcttgtcag aacagcaact gaaggaggca
    76921 ctgcaatttt tacttcccat gatagttttt atgcttgggc agtcctttac ttatttcatg
    76981 caccgctggg ccactaaaaa agggctccac tggtacttat tatcaagcgt tatcttaaca
    77041 gggattgctt ttggaatggc cctattcacg ccttttcttc cttccaatgt tacggtagca
    77101 gctttagcct tttttgcctc tattcaggtt gatacgttta aaaccttacg aggagctagc
    77161 tatgctaatg tgatgatgac aggaaatatc aaaaatgccg cctatttact aaccaaaggc
    77221 ttgtacgaaa aaaatcacga actaacacat atcggacgca acaccttaat agtcatcttg
    77281 gcttttgctg ttggggtggt ttgttcgacg ttgctttgca tagcttatgg cgagtatgct
    77341 ttgatgccta ttttaatgcc tttactctat gtcaattacc tgttggctca ggaattttat
    77401 cacattcaaa ccaaaatcaa accgattaaa agaacttaac ggacttgtgg agaaagaaag
    77461 ttcatttagg gtaagttttt acttaccctc cgttgatttg ctggttttgc tgccccttaa
    77521 aatatgatat aatagaccat tgatagctgt tacttaacag aaggctgata aaaaaggttc
    77581 cattagcctt gagggttgaa tttaaacagg aaaaaacgaa atcctacact atgcatctca
    77641 atcgtacttt catttgagta tggttctgaa aggaaactat gatgaaaaaa ccattttaca
    77701 ttacgacacc catttattac ccatctggga aattacacat tggttctgcc tatacaacta
    77761 ttgcttgtga tgtcttggca cgctacaagc gtctgatggg ccatgaggtc ttttacctca
    77821 caggactaga tgaacacgga caaaagattc aaaccaaggc aaaagaagct ggtattacac
    77881 ctcaaaccta cgttgataac atggcaaagg atgtcaaagc cctttggcaa ttgctagata
    77941 tttcctatga cacatttatc cggacaacgg acgattacca cgaagaggtc gttgctgctg
    78001 tttttgaaaa attactggcg caagatgaca tttaccttgg tgaatactct ggttggtatt
    78061 ctgtctctga tgaggaattt ttcactgaaa gccaactaga agaagttttt agagatgaag
    78121 acggtaaggt tattggaggg attgctcctt caggtcacca agtagagtgg gtatcagaag
    78181 aatcttactt cctacgcctt agcaagtacg ctgaccgcct cgtagccttc tttaaagagc
    78241 gacctgactt tatccaacca gacggtcgta tgaatgaaat ggtgaaaaac tttatcgagc
    78301 caggcctaga agatttggct gttagccgta ctacctttac atggggtgta cctgttccat
    78361 cagacccaaa acacgtggtt tatgtctgga tcgatgccct cctaaactac gcgacagctc
    78421 ttggttatgg tcaagctaat cacgctaact ttgataaatt ctggaatgga acggttttcc
    78481 acatggttgg aaaagatatc ttgcgttttc atagcatcta ctggccgatc ttactcatga
    78541 tgcttgattt accaatgcca gaccgcttaa ttgcccatgg ttggtttgtc atgaaagacg
    78601 gtaaaatgtc taaatcaaaa ggcaatgtgg tctatccaga aatgttagtg gagcgctttg
    78661 gacttgatcc tcttcgttat tacctcatgc gttctttgcc agttggttca gacgggacct
    78721 ttacccctga agattatgtg ggtcgtatca actatgagtt agccaacgac cttggcaatt
    78781 tattgaaccg taccgttgcc atgatcaaca agtattttga cggcacggtg ccagcttatg
    78841 ttgacaatgg aactgccttt gatgctgact tcagtcaact gattgatgct cagctagcag
    78901 actaccataa gcacatggaa gctgtagact acccacgcgc cttagaagcc atttggacca
    78961 tcattgctcg taccaacaaa tacatcgatg aaaccgctcc ttgggtgctc gctaaagaag
    79021 atggtgacaa agctcaacta gccagtgtca tggcacactt ggcagctagc ttgcgcgtgg
    79081 tcgctcatgt gatccaaccc tttatgatgg aaacatcagc tgctatcatg gcccagcttg
    79141 gcttagcgcc tgtttctgac ttatcaacac ttgctctagc agattttcct gccaatacaa
    79201 aggtcgttgc caagggcaca ccgattttcc cacgtcttga catggaagct gagattgact
    79261 acattaaagc ccaaatgggt gacagctcag ctatttccca agaaaaagaa tgggtccctg
    79321 aagaggttgc gctcaaatct gaaaaagacg tcatcacatt tgaaaccttt gatgctgtgg
    79381 aaatccgtgt ggcagaagtc aaggaagtgt caaaagtgga aggttcagaa aaattgctgc
    79441 gctttagagt agatgcagga gacgggcaag accgtcaaat cttgtcaggt attgccaaat
    79501 gctaccccaa cgagcaagaa ttagtgggta aaaaactaca aattgttgct aacctgaaac
    79561 cgcgtaaaat gatgaaacaa tacatcagcc aaggcatgat tttgtcagca gagcatggag
    79621 atcagctaac ggtcttaacc gttgattcct ctgttccaaa tggaagtatc attggctgat
    79681 gctgacctat tattcaaaaa cctaagcaag aatatgtgct taggtttttt gattgccttc
    79741 aagaaagatg aataacgaga tgggtgatat ggcatcaaaa gcataactat cctaggacct
    79801 gtcctagtaa gattgctgtg cttttgacaa gtgaacagag aggtcttaaa ctatatcttg
    79861 tcataaaaaa gattgcaaaa cactatatct tgtgttatta tggtttttat aaaagttaag
    79921 cgtgcttaac tttagccaaa caaaagagga cactagagcg tgtcccacaa gacagttgag
    79981 cccttcgctt ttttagtaga gtaggagtgc tcaagtcaaa aagagacagc ccatcaaacc
    80041 attgctgttt agcaggtctt tttgtgttgc tcaaaaaaca gttcctgtct gtttggcgtc
    80101 tagtgatgac ttaggcttag cgctactaga cttttgtttg aaaaatacct caagccttga
    80161 aggagacaaa tgacccaaca ttattatgaa cgttcgcaat ccccaattga atacgcccta
    80221 tctgaaacac aaaaacagtt gcgctccatc aattggaatt accttaatga tgacaaagat
    80281 ttggaagtgt ggaaccgtgt cactcaaaat ttttggttgc ctgaaaaggt tcctgtttct
    80341 aatgatttaa attcttggcg ctccttgggt gaggattggc aacagctcat cacaaggacc
    80401 tacactgggt tgaccttact agatacagta caagcaacgg ttggtgatgt ggcacaaatt
    80461 cagcattcac aaaccgacca cgaacaagtc atctatacga actttgcctt tatggtgggg
    80521 attcatgccc gctcttacgg gacgattttt tcgacccttt gcagcagcga gcaaatcgaa
    80581 gaagcacatg aatgggtggt ctcaacacag agcttgcaag accgttcccg tgtcttgatt
    80641 ccttactata cgggtgatga ccctttgaaa tcaaaagtag cagcagctat gatgccagga
    80701 tttttgctgt atggcggctt ttaccttcct ttttacctat cagcacgtgg taaaatgcca
    80761 aacacctctg atatcatccg cctgatttta cgcgacaaag ttattcacaa ctattacagc
    80821 ggttacaaat accaacaaaa agtagcacgt cttagcccag aaaaacaggc tgaaatgaag
    80881 gcctttgtct ttgacttgct ctatgaactc atcgaccttg aaaaagctta cttgagagaa
    80941 ctgtatgcag gctttgattt ggcagaagat gctatccgtt ttagccttta taacgctgga
    81001 aaattccttc aaaatttagg ttatgattcg cccttcacag atgaagaaac ccgtatctca
    81061 cctgaagtat ttgctcagct atcagcgcgt gcagacgaaa accatgactt tttctcaggc
    81121 aatggctcat cttatgtgat ggggatcacc gaagaaacca cagatgacga ttgggagttt
    81181 taagaaatgg cagagctgat tattgtgtat ttttcatcta agtccaataa cacccatcgc
    81241 ttcgtccaaa aattaggcct gcctgctcag cgcataccag tagataatag gccacttgaa
    81301 gtttcaaccc attacctttt gattgtgcct acctatgcag ctggtggttc agatgccaaa
    81361 ggagccgttc ctaaacaagt gattcgtttt ttgaataatc ctaacaatcg taaacattgt
    81421 aagggagtga tctcttcagg aaataccaat tttggcgata cctttgcctt agcaggccct
    81481 atcatttctc aaaaattgca ggtcccccta ttacaccaat ttgaactatt aggcactgct
    81541 acagatgtca aaaaggtaca ggcgatcttt gcccgcttaa agcatcacac acacgacaag
    81601 caaaaacaaa tcaacaacct gattacagaa aggacacacc catgtcacaa accaatgcgt
    81661 catacctctc attaaatgca ctgacccgtt ttaaaaaacc agacggcagc taccattttg
    81721 atagcgataa agaagctgtt aggcgctacc tggaagaaca tgtctctccc aatcaaatgg
    81781 cttttaactc actagaagat aagctagctt acctcatcaa tgaagggtat tatgagcagg
    81841 ccatttttga tgcctatcct aacgacctta tcaaggaggc ctttcattat gcctatcaac
    81901 aaggctatcg ctttttaaat ctcatgggcg ctatgaaatt ttaccagtcc tatgccttaa
    81961 aaaccttgga cggtaagcag tatctagaaa catttgaaga ccgtgctgtg atgaatgcac
    82021 tctttttagc tgatggagat caaacctttg tctttgacgt gattgatgcc atcttgcacc
    82081 gacgctttca accagctacc ccaacgtttt taaatgccgg aaaaaaacgc cgtggcgaat
    82141 acatttcgtg ctatcttctt cgtgtcgaag acaatatgga atctatctct cgcgccatta
    82201 gcaccagctt gcaactctca aaaagaggag gcggagtcgc cttgtgcctc accaacctca
    82261 gagaaatcgg agcaccgata aaagggattg aaaatcaggc aacaggcatc gttcctgtca
    82321 tgaaactact agaggactct ttttcctatg ccaatcaact aggtcagcgc caaggagcag
    82381 gtgcggttta ccttcatgcc catcatcctg aggtcttgac ctttttagac accaaacgtg
    82441 aaaatgccga tgagaaaatt cgcattaagt ccctagcatt gggacttgtg atcccagata
    82501 ttaccttcca gttagccaaa gaaaacaaag acatggctct cttttcaccc tatgatatca
    82561 agcgtgccta tggcaaggac atgtcagaca tctccatcac agaggaatac gataaactgc
    82621 tagccaatcc agccatcaaa aagacctaca tcagtgctag aaagttcttt cagttaattg
    82681 cagagctaca ttttgagtca ggctaccctt acctcttgtt tgatgacacg gtcaataagc
    82741 gcaatcctca tgccaaaaaa ggcagaatcg tcatgtctaa cctgtgttct gaaatcgctc
    82801 aagtcagcac cccaagcact ttcaaagaag atttgagttt tgaaaccatt ggagaggata
    82861 tctgctgtaa tttagggtct atcaatattg cccaggctat ggcagatgcg ccacactttg
    82921 aacagctcat cacaacgagc atcagagcac ttgatcgtgt ctctagagtg tctgacctga
    82981 attgtgcgcc ttctgttgag acaggcaatg cagccaacca tgcagtagga ttaggcgcca
    83041 tgaacctaca tgggtttttg gcaaccaacc acatctacta tgatacaaaa gaagccgttg
    83101 actttacaga tctgtttttc catgccatgg cctattatgc ttttaaagcc tcttgccaat
    83161 tagccaaaga aaaaggcgct tttgcaggat tttcgttatc aacctatgca gatggcactt
    83221 attttgccaa gtatttgcaa gaagatgcca agccacaaac agccaaggtt gccacattgt
    83281 tgcaagatta cggcttcaca ttgccaacgg tggcggattg gcaagcccta gtagcagata
    83341 tcaagcagtt tggtctagcc aatgcgcact tgctagcggt tgctccgaca ggctccatca
    83401 gctacttatc atcctgcact ccgagtctac aaccagtcgt tgctcctgtt gaggtccgaa
    83461 aagaaggtag cctgggcagg atttacgtcc ctgcctatca gattgaccag gctaactatg
    83521 cttactatga acgtggtgcc tatgaggtcg gcccaaaagc catcatcgac gttgtagctg
    83581 ctgcccaaaa acacgttgac caagccatct cattgaccct gtttatgacc gatcaagcga
    83641 ccacacgtga ccttaaccgc tcctatatcc aagcttttaa gcaaaactgt gcatcgatct
    83701 attatgtgcg agtgaggcaa gacgtcctag caggcagcga acagtatgac gaggatagtc
    83761 ttgtgacagc tcctggagct agtgatgaga ccaccacaga gtgtcaaagc tgtatgatct
    83821 aacagctact agaaagctct cgtgtcccct atcacatgca taggatcagt gcactcgacc
    83881 tttcaagaca agcaagcatc agctcttgct tgtctttttt tggccttaaa gcccgttagt
    83941 ctgctgctat gcgaggcttt ttttgagcat tagaacgtca aaaaaaagga catggagtcc
    84001 ttttttggtg atcggtgttg aggccgtcac aaactgccct tgaaatacgc ttctatgtgg
    84061 agcttttttt ggtcttgtga cacgtaagct ccaacgacct ccagctgaca gcctcttgga
    84121 aacaagagct caacctctga aggcacagcc gaataaggct cgacaaaggc tgccttggcc
    84181 ccttttttga cacagatgcg cacctccacc ggtcgatggg tcatggcgcc gtttttcaag
    84241 gctgtcgtgc tcataaaact gtgcttggtg tagcgtgtgc caagcttgat cttacaaagg
    84301 atatgagggt caaactgatg gttacggtag taagacgtga gatcagcgtg tgaaacaccg
    84361 atatcacgca aaaacgtctc gtacacatag cggtatacca caatgttcca cgggatgact
    84421 agccggtgag tcgcagcatc gagctgggcc acctgatccc ttagctcagg cgtgagttgg
    84481 ctgagctcac ctttggtctt gtctaggctg gtgttgatag gggctgcctg ctggcaggta
    84541 taagcagcca tcgcctcttg ctcagccctt gggtagacga gacccttgcc ccagcgcttt
    84601 ttaatcagtg tctcagcgcc cttttgatcc gaaaaggtaa aataagcagg cgtcccctca
    84661 caaaccgccc gctcatagtg ctcacagaca aaaagctcga ccaagccctc agtttgccag
    84721 actagactaa aacagctaag aagaaggatc atagccagtt gatagcgctt ttttagcata
    84781 gttccccctg acaccagccc aaaaggctga cccttcccct aggccttttg gctagctcat
    84841 gataccacca gtttctcaga caggctaatc tttttgtcat ctctttctcc ctaaacagac
    84901 tctcttatca tgatattatg ctattatgag cgatttgtca atccatagca accgtttttt
    84961 agccagataa gtaagctttt gacctagagg cagacaagag tggcagtacc tgccgtttgc
    85021 tgtggtggct aataaggact aacataaaat atcaaacgct ttcaaaaaca gctcctaagc
    85081 ttaaaaaagc tgatgaacaa ttttttgagt ttaagagaga ttgccatgat tttctaatca
    85141 tttgatgttt aaaatgataa aaacatgaac ttgaaatgaa agaatacgag tagtaaacta
    85201 aaaat
//
LOCUS       DATXIG010000004        71133 bp    DNA     linear   BCT 28-DEC-2023
DEFINITION  TPA_asm: Streptococcus pyogenes strain MGAS11113
            SAMN08555799-rid19682583.denovo.004, whole genome shotgun sequence.
ACCESSION   DATXIG010000004 DATXIG010000000
VERSION     DATXIG010000004.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN08555799
            Sequence Read Archive: SRR6740495
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pyogenes
  ORGANISM  Streptococcus pyogenes
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 71133)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 71133)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-OCT-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-OCT-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001918058.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001918058.2
            Genome Coverage       :: 368x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/12/2023 11:23:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 1,806
            CDSs (total)                      :: 1,769
            Genes (coding)                    :: 1,722
            CDSs (with protein)               :: 1,722
            Genes (RNA)                       :: 37
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1, 1 (5S, 23S)
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 47
            CDSs (without protein)            :: 47
            Pseudo Genes (ambiguous residues) :: 0 of 47
            Pseudo Genes (frameshifted)       :: 30 of 47
            Pseudo Genes (incomplete)         :: 20 of 47
            Pseudo Genes (internal stop)      :: 9 of 47
            Pseudo Genes (multiple problems)  :: 11 of 47
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..71133
                     /organism="Streptococcus pyogenes"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN08555799-rid19682583.denovo.004"
                     /strain="MGAS11113"
                     /serotype="Emm28"
                     /isolation_source="normally sterile site"
                     /host="Homo sapiens"
                     /db_xref="taxon:1314"
                     /country="Finland"
                     /collection_date="2002"
                     /collected_by="National Public Health Laboratory"
     gene            complement(127..2034)
                     /locus_tag="VC122_000239"
     CDS             complement(127..2034)
                     /locus_tag="VC122_000239"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011017710.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC-F family ATP-binding cassette
                     domain-containing protein"
                     /protein_id="HEP5640197.1"
                     /translation="MIILQGNKLERSFSGDVLFQNISLQVDERDRIALVGPNGAGKST
                     LLKLLVGEETPTSGEVNTKKDLTLSYLAQNSRFESDKTIYEEMLKVFEALRQDEKRLR
                     QMEMDMATVSGQDLTRLMMDYDLLAERFRQQGGFTYEAEIKAILNGFKFDESMWQMTI
                     AELSGGQNTRLALAKMLLEKPELLVLDEPTNHLDIETIAWLENYLANYQGALIIVSHD
                     RYFLDKVATVTLDLTPHGLDRYVGNYSRFMTLKAEKLVAEEKQFDKQQKEIAKLEDFV
                     QKNIVRASTTKRAQARRKQLEKIERLDKPTSARKSAHMTFQADKPSGNVVLTVEKAAI
                     GYNQHVLSEPINLDVHKLDAIAIVGPNGIGKSTLIKSVIGQIPFIKGEVKYGANVEIG
                     YYDQTQSHLTSSNTVLEELWQDFSTTPEVDIRNRLGAFLFSGDDVKKSVAMLSGGEKA
                     RLLLAKLSMENNNFLVLDEPTNHLDIDSKEVLENALIDFDGTLLFVSHDRYFINRLAT
                     KVLEITENGSTLYLGDYDYYLEKKAELEELARLAAGETVEETKEASATDYQLQKANQK
                     ERRRLTRRYEEIEARLETIEERIGAIQEDMHASNDTAQLIAWQKEWDQLDQEQETLME
                     EWETIAEQIES"
     gene            complement(2080..2406)
                     /locus_tag="VC122_000240"
     CDS             complement(2080..2406)
                     /locus_tag="VC122_000240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009880822.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEP5640198.1"
                     /translation="MKQLLAIILWLPKLIVKMFWHLIKGFLQTILLVTIIIIGLMYYS
                     NHSDSVLANKISIVTEQVVQIFDILTQKPSAKTRHGSGNSHSTFLVSAKEGNQRLKKS
                     SISFGN"
     gene            complement(2436..3221)
                     /locus_tag="VC122_000241"
     CDS             complement(2436..3221)
                     /locus_tag="VC122_000241"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009880823.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="esterase family protein"
                     /protein_id="HEP5640199.1"
                     /translation="MASIAIEYHSVVLGMERKVNVIYPDQSEIPKKDQGDKDIPVLYL
                     LHGMGGNENSWQKRTAIERLLRHTNLIVVMPSTDLGWYTDTAYGLNYYRALSQELPQV
                     LAAFFPNMTQKREKTFVAGLSMGGYGAFKWALKSNRFSYAASFSGALDFSPETLLEGN
                     LGELAYWQGVFGQFDDPDLDKHYLKNMVAESDGKTKFYAWCGYEDFLFATNEKAIADF
                     QAQGLDIDYHKGHGKHEWYYWNQQLEVLLEWLPINYQKEERLS"
     gene            complement(3354..5015)
                     /locus_tag="VC122_000242"
     CDS             complement(3354..5015)
                     /locus_tag="VC122_000242"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017646201.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease J"
                     /protein_id="HEP5640200.1"
                     /translation="MTDIKMIALGGVREYGKNFYLVEINDSMFILDAGLKYPENEQLG
                     VDLVIPNLDYVIENKGKVQGIFLSHGHADAIGALPYLLAEVSAPVFGSELTIELAKLF
                     VKSNNSTKKFNNFHVVDSDTEIEFKDGLVSFFRTTHSIPESMGIVIGTDKGNIVYTGD
                     FKFDQAAREGYQTDLLRLAEIGKEGVLALLSDSVNATSNDQIASESEVGEEMDSVISD
                     ADGRVIVAAVASNLVRIQQVFDSATAHGRRVVLTGTDAENIVRTALRLEKLMITDERL
                     LIKPKDMSKFEDHELIILEAGRMGEPINSLQKMAAGRHRYVQIKEGDLVYIVTTPSTA
                     KEAMVARVENLIYKAGGSVKLITQNLRVSGHANGRDLQLLMNLLKPQYLFPVQGEYRD
                     LAAHAKLAEEVGIFPENIHILKRGDIMVLNDEGFLHEGGVPASDVMIDGNAIGDVGNI
                     VLRDRKVLSEDGIFIVAITVSKKEKRIISKAKVNTRGFVYVKKSHDILRESAELVNTT
                     VGNYLKKDTFDWGELKGNVRDDLSKFLFEQTKRRPAILPVVMEVR"
     gene            complement(5219..5977)
                     /locus_tag="VC122_000243"
     CDS             complement(5219..5977)
                     /locus_tag="VC122_000243"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002991977.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEP5640201.1"
                     /translation="MTKIIELINATVDVDNGFEDAKTILDNVTLTIYEHDFLTILGGN
                     GAGKSTLFNVIAGTLSLTRGQIRILGQDVTHWPAEKRALYLSRVFQDPKMGTAPRMTV
                     AENLLIARQRGEKRSLASRKITEHLASFEDLVKRTGNGLEKHLETPAGLLSGGQRQAL
                     SLLMATLKKPALLLLDEHTAALDPKTSQSLMQLTDEFVTKDGLTALMITHHMEDALTY
                     GNRLIVMKDGNIIKDLNQMEKEQLTITDYYQLFD"
     gene            complement(5974..6843)
                     /locus_tag="VC122_000244"
     CDS             complement(5974..6843)
                     /locus_tag="VC122_000244"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002991975.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEP5640202.1"
                     /translation="MIISSVSQGLIWGVLGLGIYLTFRILNFPDMTTEGSFPLGGAVA
                     VTAISLGWNPFLSTLLGMLSGALAGFLTGLLYTKGKMPTLLAGILVMTSCNSIMLMVM
                     GRANLGLHDHKRIQDCLPFSIDLNSLLTGLITVVIVISVLIYFLYTNLGQAYIATGDN
                     KDMAKSFGINTDWMEVMGLVVSNSLIALSGALVSQQDGYADVSKGIGVIVIGLASIIV
                     GEVLYSTGLTLLERLIAIVIGSILYQFLISVVITLGFNTSYLKLISALVLALCLMIPV
                     VKERYFKGVRLTR"
     gene            complement(7188..8186)
                     /locus_tag="VC122_000245"
     CDS             complement(7188..8186)
                     /locus_tag="VC122_000245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002989997.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HEP5640203.1"
                     /translation="MKNKSLIATLLVLTVIVIGSLLSKGVSKENRDLANRQNITIGIL
                     QFVTHEALDDIKRGIEDQLKKQMPQKQNVVIKVMNAEGDQSKIQTMSRQLVQSGSDIV
                     IGIATPAAQGLAAASKDIPVVMSAVSDPVGSRLVMQLDQPEANVTGLSNKVPVKQTID
                     LMKNLTPHVKTVGILYASNEDNSLSQVKEFRRLARKKGYQVISYAVPSTNEVPATMSV
                     MLGKVDAVFIPQDNTIASAFSSVMTTSKAAKIPVYTSVDRMVEKGGLAAISQNQYDLG
                     VQTANQVLKLIKGKRVIDVPVKVVDIGQPLINKNVAAELGIAINREDFPSASFIEN"
     gene            8540..10192
                     /gene="fbp54"
                     /locus_tag="VC122_000246"
     CDS             8540..10192
                     /gene="fbp54"
                     /locus_tag="VC122_000246"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010922232.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rqc2 family fibronectin-binding protein Fbp54"
                     /protein_id="HEP5640204.1"
                     /translation="MSFDGFFLHHLTNELKENLLYGRIQKVNQPFERELVLTIRNHRK
                     NYKLLLSAHPVFGRVQITQADFQNPQVPNTFTMIMRKYLQGAVIEQLEQIDNDRIIEI
                     KVSNKNEIGDAIQATLIIEIMGKHSNIILVDRAENKIIESIKHVGFSQNSYRTILPGS
                     TYIEPPKTAAVNPFTITDVPLFEILQTQELTVKSLQQHFQGLGRDTAKELAELLTTDK
                     LKRFREFFARPTQANLTTASFAPVLFSDSHATFETLSEMLDHFYQDKAERDRINQQAS
                     DLIHRVQTELDKNRNKLSKQEAELLATENAELFRQKGELLTTYLSLVPNNQDSVILDN
                     YYTGEKIEIALDKALTPNQNAQRYFKKYQKLKEAVKHLSGLIADTKQSITYFESVDYN
                     LSQASIDDIEDIREELYQAGFLKSRQRDKRHKRKKPEQYLASDGKTILMVGRNNLQNE
                     ELTFKMAKKGELWFHAKDIPGSHVIIKDNLDPSDEVKTDAAELAAYYSKARLSNLVQV
                     DMIEAKKLHKPSGAKPGFVTYTGQKTLRVTPDQAKILSMKLS"
     gene            complement(10251..11498)
                     /locus_tag="VC122_000247"
     CDS             complement(10251..11498)
                     /locus_tag="VC122_000247"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003043897.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HEP5640205.1"
                     /translation="MLNSEKMIASLDQQDLAHAEKYFQKALKEDDADSLIALGEYLES
                     IGFLPHAKRIYLQLADDYPELNINLAQIAAEDDAIEEAFLYLDKVSKDSPNYLSALLV
                     MADLYDMEGLTEVAREKLLQAVSISPEPLVIFGLAEIDMSLQYFKEAIDYYAQLDNRQ
                     ILELTGISTYQRIGRAYASLGKFEAAIEFLEKAVAIEYEDETVFELATLLYDQENYQK
                     ANLYFKQLETINPDYPGYEYGYALSLHEEHKTSEALRLVQQGLRKNAFDSQLLLLASQ
                     LSYELHDRQNAENYLLQAKEVAVDDEEILMRLVTLYFDAERFEEVIALNRETIDNVLT
                     KWTIAKAYHALEQEEVALALYNEISADLAENPEFLQDYAYLLREFGQFHKAIQMATAY
                     LRQVPDDVNMQDFLDHIKHQQSE"
     gene            complement(11488..12669)
                     /locus_tag="VC122_000248"
     CDS             complement(11488..12669)
                     /locus_tag="VC122_000248"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002990002.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AI-2E family transporter"
                     /protein_id="HEP5640206.1"
                     /translation="MPLIPDKSRTDSLFYKWFLNNQATMALVITLLAFLTIFVFTKIS
                     FLFMPVISFFAVIMLPLVISTILYYLTKPLVDLINHLGPNRTTSIFIVFGLITLLFVW
                     AISGFVPMVQTQLTSFIEDLPKYVGKVNEEANKLLENEWLVSYKPQLQDMLTHTSQKA
                     LDYAQSFSKNAIDWAGNFAGAIARITVAIIISPFILFYFLRDSSHMKNGLVNVLPLKL
                     RVPMVRVLGDINKQLSGYVQGQVTVAIVVGFMFSIMFSLVGLKYAITFGIIAGFLNMI
                     PYLGSFLAMIPVVIMAMVQGPFMLVKVLVIFMIEQTIEGRFVAPLVLGNKLSIHPITI
                     MFLLLTAGSMFGVWGVFLVIPIYASVKVVIKELFDWYKKVSGLYDEEVLVIEEVKDHV
                     K"
     gene            complement(12727..13203)
                     /locus_tag="VC122_000249"
     CDS             complement(12727..13203)
                     /locus_tag="VC122_000249"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010922230.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="8-oxo-dGTP diphosphatase"
                     /protein_id="HEP5640207.1"
                     /translation="MTQLATICYIDNGDSLLLLHRNKKENDVHKGKWISVGGKLEAGE
                     TPDECARREILEETHLTVTEMAFKGIITFPEFTPGHDWYTYVFKVTGFEGDLISDEES
                     REGTLEWVPYDQVLEKPTWEGDYDIFKWILEDRPFFSAKFTYDQNNQLMDKSVTFY"
     gene            complement(13424..14464)
                     /gene="rfbB"
                     /locus_tag="VC122_000250"
     CDS             complement(13424..14464)
                     /gene="rfbB"
                     /locus_tag="VC122_000250"
                     /EC_number="4.2.1.46"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000277597.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dTDP-glucose 4,6-dehydratase"
                     /protein_id="HEP5640208.1"
                     /translation="MYKNIIVTGGAGFIGSNFVHYVYNNHPDVHVTVLDKLTYAGNRA
                     NIEAILGDRVELVVGDIADAELVDKLAAKTDAIVHYAAESHNDNSLEDPSPFIHTNFI
                     GTYTLLEAARKYDIRFHHVSTDEVYGDLPLREDLPGQGEGPGEKFTAETKYNPSSPYS
                     STKAASDLIVKAWVRSFGVKATISNCSNNYGPYQHIEKFIPRQITNILSGIKPKLYGE
                     GKNVRDWIHTNDHSTGVWAILTKGRIGETYLIGADGEKNNKEVLELILEKMGQPKDAY
                     DHVTDRAGHDLRYAIDSTKLREELGWEPQFTNFSEGLEETIKWYTENETWWKAEKDAV
                     EAKYAKTQEVIK"
     gene            complement(14708..15301)
                     /locus_tag="VC122_000251"
     CDS             complement(14708..15301)
                     /locus_tag="VC122_000251"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018379055.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dTDP-4-dehydrorhamnose 3,5-epimerase"
                     /protein_id="HEP5640209.1"
                     /translation="MTETFFDKPLACREIKEIPGLLEFDIPVRGDNRGWFKENFQKEK
                     MLPIGFPERFFEEGKLQNNVSFSRQHVLRGLHAEPWDKYISVADDGKVLGAWVDLREG
                     ETFGNVYQTVIDASKGMFVPRGVANGFQVLSETVSYSYLVNDYWALDLKPKYAFVNYA
                     DPSLGITWENLAAAEVSEADKNHPLLSDVKPLKPKDL"
     gene            complement(15301..16170)
                     /gene="rfbA"
                     /locus_tag="VC122_000252"
     CDS             complement(15301..16170)
                     /gene="rfbA"
                     /locus_tag="VC122_000252"
                     /EC_number="2.7.7.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296172.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-1-phosphate thymidylyltransferase RfbA"
                     /protein_id="HEP5640210.1"
                     /translation="MKGIILAGGSGTRLYPLTRAASKQLMPIYDKPMIYYPLSTLMLA
                     GIKDILIISTPQDLPRFEELLGDGSEFGISLSYKEQPSPDGLAQAFIIGEEFIGDDRV
                     ALILGDNIYHGNGLTKMLQKAAAKEKGATVFGYQVKDPERFGVVEFDENMNAISIEEK
                     PEVPKSHFAVTGLYFYDNDVVEIAKNIKPSARGELEITDVNKAYLERGDLSVELMGRG
                     FAWLDTGTHESLLEAAQYIETVQRLQNAQVANLEEIAYRMGYISKEDVHELAQSLKKN
                     EYGQYLLRLIGEA"
     gene            complement(16229..17335)
                     /locus_tag="VC122_000253"
     CDS             complement(16229..17335)
                     /locus_tag="VC122_000253"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010922194.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-binding oxidoreductase"
                     /protein_id="HEP5640211.1"
                     /translation="MKIAIIGAGIVGSTAAYYLQQSGQKEVTIFDHGQGQATKAAAGI
                     ISPWFSKRRNKVWYRMARLGADFYQQLINDLKEDGFATDFYQQNGIYVLKKQEEKLRD
                     LYELALARKVESPIIGELAIKNRKELGDDFKGLIGFDNCLYASGAARVEGAALCETLL
                     KTSGYPVIRQKVTLKQQGSGYEIAGHYFDQVILAAGAWLPDLLMPLGYQVDVRPQKGQ
                     LLDYDVHHIISDNYPVVMPEGEIDLIPFNQGKISVGASHENDKGYDLEPDWQVLEKLE
                     MQALTYLPLLKEATQKSCRVGIRAYTSDYSPFYGQVPGLKNLYTASGLGSSGLTVGPL
                     IGYELAQLLLGHEGLLTPSDYSPEPYLLRSTGKA"
     gene            complement(17375..18163)
                     /locus_tag="VC122_000254"
     CDS             complement(17375..18163)
                     /locus_tag="VC122_000254"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019781659.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Nif3-like dinuclear metal center hexameric
                     protein"
                     /protein_id="HEP5640212.1"
                     /translation="MKAKTLIDAYEAFCPLDLSMERDVKGLQMGSLDKDIRKVMITLD
                     IRESTVAEAIKNEVDLIITKHAPIFKPLKDLVSSPQRDILLDLVKHDISVYVSHTNID
                     IVPGGLNDWFCDLLEIKEATYLSETKEGFGIGRIGTVKEQALEELASKVKRVFDLDTV
                     RLIRYDKENPLISKIAICGGSGGEFYQDAVQKGADVYITGDIYYHTAQEMLTEGLFAV
                     DPGHHIEVLFTEKLKEKLQGWKEENGWDVSIISSKASTNPFSHL"
     gene            complement(18153..18839)
                     /locus_tag="VC122_000255"
     CDS             complement(18153..18839)
                     /locus_tag="VC122_000255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011054397.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (adenine-N(1))-methyltransferase"
                     /protein_id="HEP5640213.1"
                     /translation="MDSQLSNRLAQVAAYVPKGVKLLDVGSDHAYLPIFLVETNQISA
                     AIAGEVVRGPYESALKNVTQSGLAEHIQVRLANGLAAFEEADDVTAITICGMGGRLIT
                     DILEAGKEKLQGIERLVLQPNNREDDLRAWLSVNAFKIVAETIMAENDKYYEIIVAEH
                     GEKALSATELRFGPYLSQEKSVVFKEKWQREMDKLAYALSCIPEEKTQERQLLLTKIQ
                     QIKEVIVHES"
     gene            complement(18911..19567)
                     /gene="nth"
                     /locus_tag="VC122_000256"
     CDS             complement(18911..19567)
                     /gene="nth"
                     /locus_tag="VC122_000256"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002990106.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease III"
                     /protein_id="HEP5640214.1"
                     /translation="MRIGKARLAKVLTIIGQMFPEAKGELDWETPFQLLIAVILSAQT
                     TDKAVNKVTPGLWQSYPEIEDLAFAELSDVENALRTIGLYKNKAKNIIKTAQAIRDDF
                     KGQVPKTHKELESLPGVGRKTANVVLAEVYGVPAIAVDTHVARVSKRLNISSPDADVK
                     QIEADLMAKIPKKDWIITHHRLIFFGRYHCLAKKPKCEICPVQSYCKYYQNTYGKSKA
                     "
     gene            complement(19564..20247)
                     /locus_tag="VC122_000257"
     CDS             complement(19564..20247)
                     /locus_tag="VC122_000257"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011184446.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DnaD domain-containing protein"
                     /protein_id="HEP5640215.1"
                     /translation="MSFLEHYKSGNLVIPSALLFHYKDLFKSSDDFLVWQFFYLQNTT
                     KRDDLAPSQIAHALGKSVADINKIISSLTSQGLLDMRTIELTGEIEIIFDASPVLAKL
                     DQLFVSQTATEIDKQETPNHFKRLVDEFERELGRFLSPFELEDLEKTLRDDKTDPDLI
                     REALKEAVFNGKTNWKYIQAILRNWRKEGIVNLRQVEERRRVREGEDLSQVTISEDFL
                     SAMNLWSDS"
     gene            complement(20328..20846)
                     /locus_tag="VC122_000258"
     CDS             complement(20328..20846)
                     /locus_tag="VC122_000258"
                     /EC_number="2.4.2.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014294327.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenine phosphoribosyltransferase"
                     /protein_id="HEP5640216.1"
                     /translation="MDLTNYIASIKDYPKAGITFRDISPLMADGKAYSYAIREIAQYA
                     CDKDIDMVVGPEARGFIIGCPVAVELGIGFAPVRKPGKLPRDVVSADYEKEYGLDTLT
                     MHADAIKPGQRVLIVDDLLATGGTVKATIEMIEKLGGIVAGCAFLIELEGLNGRHAIR
                     NYDYKVLMQFPG"
     gene            complement(20996..23206)
                     /gene="recJ"
                     /locus_tag="VC122_000259"
     CDS             complement(20996..23206)
                     /gene="recJ"
                     /locus_tag="VC122_000259"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014407518.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="single-stranded-DNA-specific exonuclease RecJ"
                     /protein_id="HEP5640217.1"
                     /translation="MIKSKYSWKIKDKKPDDGFFKLAKTKGLTQTAAQLIYERGIRTE
                     EALDEFLTADLSQLHDPYLLHDMAKAVPRIRQAIEEGERILIYGDYDADGMTSASIVK
                     ETLDMMGAEPLVYLPNRFTDGYGPNQSVYKYFIEQEAVSLIITVDNGVAGHEAIRYAQ
                     EQEVDVIVTDHHSLPEELPEAFAIIHPEHPDADYPFKHLAGCGVAFKLATALLESLPT
                     DCLDLVAIGTIADMVSLTGENRVLVKNGLAMLKHTERVGLQELMSLSPIDLEHFNEDA
                     IGFQIAPQLNALGRLDDPNPAIELLTGFDDQEAQAIALMIKKKNEERKALVQDIFDQA
                     MAMVDPQKPVQVLAQAGWHPGVLGIVAGRIMETIGQTVVVLTIDNGFAKGSARSLEAI
                     NIFEALNGKRELFTAFGGHAGAAGMTLPVDNLEALSDFLCQFVIERGLDQTAKNTLTI
                     DERLSLDDLSLDILKSLDKLAPYGMDHQKPVFYVKDIRVSQARTIGQDQSHLKFKVSQ
                     GKASFDVLAFGQGSQLQEFRQATGLELAVTLSVNHWNGNTSLQFMLVDARVDGVQLLD
                     LRTKTAKVPEGIPTIEEDPNARVILINDIPEDFKTWRNQFVHKDFDAIYFKNQMKHPY
                     YLTGFGSREQFAKLYKTIYQFPEFDLRHKLTELSHYLNIEKLLLIKLIQIFEELSFVT
                     IDDGLMTVNPQAQKREISESHIYQDLKELVKFQEIMALASPKEMYDYLVKSNDQ"
     gene            complement(23203..23967)
                     /locus_tag="VC122_000260"
     CDS             complement(23203..23967)
                     /locus_tag="VC122_000260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003061843.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HEP5640218.1"
                     /translation="MAQRIIVITGASGGLAQAIVKQLPKEDSLILLGRNKERLEHCYQ
                     HIDNKECLELDITNPVAIEKMVAQIYQRYGRIDVLINNAGYGAFKGFEEFSAQEIADM
                     FQVNTLASIHFACLIGQKMAEQGQGHLINIVSMAGLIASAKSSIYSATKFALIGFSNA
                     LRLELADKGVYVTTVNPGPIATKFFDQADPSGHYLESVGTFTLQPNQVAKRLVSIIGK
                     NKRELNLPFSLAVTHQFYTLFPKLSDYLARKVFNYK"
     gene            complement(23967..24896)
                     /locus_tag="VC122_000261"
     CDS             complement(23967..24896)
                     /locus_tag="VC122_000261"
                     /EC_number="3.1.26.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012677753.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease Z"
                     /protein_id="HEP5640219.1"
                     /translation="MELQFLGTGAGQPAKQRNVSSLALKLLDEINEVWMFDCGEGTQR
                     QILETTIKPRKIRKIFITHLHGDHIFGLPGFLSSRSFQASEEQTDLDIYGPIGIKTYV
                     LTSLKVSGARVPYQIHFHEFDDKSLGKIMETDKFVVYAERLAHTIFCMGYRVVQKDLE
                     GTLDAEALKAAGVPFGPLFGKIKNGQDVELEDGRLICAKDYISAPKKGKIITIIGDTR
                     KTSASVKLAKDADVLVHESTYGKGDERIARNHGHSTNMQAAQIAHEAGAKRLLLNHVS
                     ARFLGRDCRQMEKDAATIFENVKMVQDLEEVII"
     gene            complement(24911..25546)
                     /locus_tag="VC122_000262"
     CDS             complement(24911..25546)
                     /locus_tag="VC122_000262"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003043928.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cystathionine beta-lyase"
                     /protein_id="HEP5640220.1"
                     /translation="MNNYIDLAKTYGGFTSLDTNYLNHLLASLTDQQKLAFITPPPSV
                     INAYFAEIYQKQSPQAATDYYFNLSKALGLFTDQPSFEEEKPFVRLNLSGKAYGFAYQ
                     NDQEVALVFSEKAEPKKPELFFELTQIFPQYMVYEDKGQLKMQAKQFEQGECEDITPD
                     DTLLSKIYRLANGITMLKGFNVEELWALSQTFSGQKYYDFAQREFMIYITQ"
     gene            complement(25539..26777)
                     /gene="hflX"
                     /locus_tag="VC122_000263"
     CDS             complement(25539..26777)
                     /gene="hflX"
                     /locus_tag="VC122_000263"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000573352.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GTPase HflX"
                     /protein_id="HEP5640221.1"
                     /translation="MIETKRQQERVILLGVELQTTEHFDMSMTELANLAKTAGVKVMA
                     SFSQKRERYDSKTFIGSGKLDEIKAIVEADEIDAVIVNNRLTARQNANLEAVLEVKVI
                     DRMQLILDIFAMRARSHEGKLQVHLAQLKYMLPRLVGQGIMLSRQAGGIGSRGPGESQ
                     LELNRRSIRHQIADIERQLTQVEKNRQTIRDRRVGSDTFKIGLIGYTNAGKSTIMNLL
                     TDDSHYEANELFATLDATTKQLYLENQFQATLTDTVGFIQDLPTELVAAFKSTLEESK
                     YVDLLLHVIDASDPNHSEQEKVVLNLLKELDMLNIPRLAIYNKVDIAEQFTATAFPNI
                     RISARSKDSKILLRRLIIDQIRDQFVPFRIKVHQDKAYKLYDLNRVALLDHYTFDQEI
                     EDISGYISPKQQWRLDDFYE"
     gene            complement(26850..27749)
                     /gene="miaA"
                     /locus_tag="VC122_000264"
     CDS             complement(26850..27749)
                     /gene="miaA"
                     /locus_tag="VC122_000264"
                     /EC_number="2.5.1.75"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012560634.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (adenosine(37)-N6)-dimethylallyltransferase
                     MiaA"
                     /protein_id="HEP5640222.1"
                     /translation="MTKIKIVVIVGPTAVGKTALGISLAKAFNGEIISGDSQQVYRQL
                     DIGTAKATQEEQEAAVHHLTDIREVTESYSAYDFVQDAQKAISDIVSRGKLPIIVGGT
                     GLYLQSLLEGYHLGGQVDQEAVKAYRNELEQLDDHDLYERLQVNNITIEQVNRRRAIR
                     ALELAQFADELENAETAYEPLIIGLNDDRQVIYDRINQRVDRMLENGLLEEAKWLYEH
                     YPTVQASRGIGYKELFPYFVGEMTLAEASDQLKQNTRRFAKRQLTWFRNRMAVSFTAI
                     TAPDYPQVVHDRVRDFLGQKEKS"
     gene            27877..28053
                     /locus_tag="VC122_000265"
     CDS             27877..28053
                     /locus_tag="VC122_000265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002263068.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3042 family protein"
                     /protein_id="HEP5640223.1"
                     /translation="MIMEYQFVKGLATGVLATAATVAGAVFAVKKTIIDPEEEKAAFI
                     EENRKKAARRRVAR"
     gene            complement(28171..29079)
                     /locus_tag="VC122_000266"
     CDS             complement(28171..29079)
                     /locus_tag="VC122_000266"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014635421.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TDT family transporter"
                     /protein_id="HEP5640224.1"
                     /translation="MKHLKNPPLVMSGLALGTLSFGNLLATYVSIFSYLGILAALFIY
                     GILLVGMVRNLNDTKMQLRQPLIASVFPTFFMTGMLLSSLFLKVTGGCWLGFLTWWLF
                     FLGNLVLIAYYQYRFVFSFSWDNVFPSWSVLFVGIAMAALTAPASRQFLLGQVIFWVC
                     LLLTAVILPFMAKKTYGIGLGQAVMPNISTFCAPLSLLSASYLATFPRPQVGMVIFLL
                     ISSQLLYAFVVVQLPRLLNRPFNPGFSAFTFPFVISATSLKMTLSFLGWQGLGWQVLL
                     LGEVLLATALVTYVYGAYLRFLFQNK"
     gene            complement(29138..29524)
                     /locus_tag="VC122_000267"
     CDS             complement(29138..29524)
                     /locus_tag="VC122_000267"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011054392.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione S-transferase family protein"
                     /protein_id="HEP5640225.1"
                     /translation="MLFIAKQTLKSQLIPQDNLLAESRFNEIMDFLTGDFPLVFRPMY
                     NPHRYTISQDNQALEKVKQASYKRMDIAMTHLDGLIGDSGHVYRDQQTIADAYAYAMA
                     LWSQKTPKSYENYPHLAAFMAKMLEM"
     gene            complement(29835..30821)
                     /locus_tag="VC122_000268"
     CDS             complement(29835..30821)
                     /locus_tag="VC122_000268"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002990124.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhodanese-related sulfurtransferase"
                     /protein_id="HEP5640226.1"
                     /translation="MSEKIRVLLYYKYVSIENAQEYAAKHLEFCKSIGLKGRILIADE
                     GINGTVSGDYETTQKYMDWVHSDERFADLWFKIDEENQQAFRKMFVRYKKEIVHLGLE
                     DNNFDSDINPLETTGEYLNPKQFKEALLDEDTVVLDTRNDYEYDLGHFRGAIRPDIRN
                     FRELPQWVRDNKDKFMEKRVVVYCTGGVRCEKFSGWMVREGFKDVGQLHGGIATYGKD
                     PEVQGELWDGAMYVFDDRISVPINHVNPTVISKDYFDGTPCERYVNCANPFCNKQIFA
                     SEENETKYVRGCSPECRAHERNRYVQENGLSRQGWAERLEAIGESLPEFVGA"
     gene            31021..31323
                     /locus_tag="VC122_000269"
     CDS             31021..31323
                     /locus_tag="VC122_000269"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011888962.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEP5640227.1"
                     /translation="MTYELCLEYGTYPLSRVYAYWGEDQNPPTFIQEDRLLCHKLETM
                     NHLFHDLFVTIESQFHYVGFNMPEKRAQIRILYQEVATILKSKYKDYPIKIETFLL"
     gene            complement(31686..31757)
                     /locus_tag="VC122_000270"
     tRNA            complement(31686..31757)
                     /locus_tag="VC122_000270"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(31722..31724),aa:Arg,seq:ccg)
     gene            complement(31870..33075)
                     /gene="rpsA"
                     /locus_tag="VC122_000271"
     CDS             complement(31870..33075)
                     /gene="rpsA"
                     /locus_tag="VC122_000271"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002997242.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S1"
                     /protein_id="HEP5640228.1"
                     /translation="MNEFEDLLNSVSEVNPGDVVTAEVLTVDNGQANVVIEGTGVEGV
                     LTLRELTNDRDADINDFVKAGDTVEVLVLRQVVGKDTDTVTFLVSKKRLEARKAWDKL
                     VGREGEVVTVKGTRAVKGGLSVEFEGLRGFIPASMIDTRFVRNTEKFVGQEFDAKIKE
                     VDAAENRFILSRREVIEEAAKEARAEVFSKISEGAVVTGTVARLTSFGAFIDLGGVDG
                     LVHVTELSHERNVSPKSVVSVGEEVEVKVLSIDEEAGRVSLSLKATTPGPWDGVEQKL
                     AQGDVVEGKVKRLTDFGAFVEVLPGIDGLVHISQISHKRVENPKDVLSVGQEVTVKVL
                     EVNAADERVSLSIKALEERPAQAEGDNKEEKRQSRPRRPKRESRRDYELPETQTGFSM
                     ADLFGDIEL"
     gene            complement(33202..33273)
                     /locus_tag="VC122_000272"
     tRNA            complement(33202..33273)
                     /locus_tag="VC122_000272"
                     /product="tRNA-Gln"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(33239..33241),aa:Gln,seq:ttg)
     gene            complement(33324..33404)
                     /locus_tag="VC122_000273"
     tRNA            complement(33324..33404)
                     /locus_tag="VC122_000273"
                     /product="tRNA-Tyr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(33368..33370),aa:Tyr,seq:gta)
     gene            complement(33465..33695)
                     /locus_tag="VC122_000274"
     CDS             complement(33465..33695)
                     /locus_tag="VC122_000274"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015057615.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2969 domain-containing protein"
                     /protein_id="HEP5640229.1"
                     /translation="MSKKDKKIEIQLIDHKVMVNETKIDGYQLQIGKRVVGEIAELDE
                     KFAVLKNDGVDCFFKTLEQAVENIIENYNLNH"
     gene            complement(33759..34826)
                     /locus_tag="VC122_000275"
     CDS             complement(33759..34826)
                     /locus_tag="VC122_000275"
                     /EC_number="2.6.1.42"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011528530.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid aminotransferase"
                     /protein_id="HEP5640230.1"
                     /translation="MKKSYNRKKQKRGTMMTIAIDWDNLGFEYHKLPFRYISYYKNGQ
                     WDKGQLTEDATLHISESAPALHYGQQAFEGLKAYRTKDGSIQLFRPDRNAVRLQATAD
                     RLLMPQVSTEQFIDAAKQVVKANEDFVPPYGTGATLYLRPLLIGVGDIIGVKPAEEYI
                     FTIFAMPVGNYFKGGLAPTNFIVSEAFDRAAPYGTGAAKVGGNYAGSLLPGKAAKSAG
                     FSDVIYLDPATHTKIEEVGAANFFGITANNEFVTPLSPSILPSITKYSLLQLAEERLG
                     MTVIEGDVPINELDKFVEAGACGTAAVISPIGGIQYKDNLHVFYSETEVGPVTRRLYD
                     ELVGIQFGDIEAPEGWIVKVD"
     gene            complement(34904..37363)
                     /gene="parC"
                     /locus_tag="VC122_000276"
     CDS             complement(34904..37363)
                     /gene="parC"
                     /locus_tag="VC122_000276"
                     /EC_number="5.6.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_021340535.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase IV subunit A"
                     /protein_id="HEP5640231.1"
                     /translation="MSNIQNMSLEDIMGERFGRYSKYIIQERALPDIRDGLKPVQRRI
                     LYSMNKDGNTFEKGYRKSAKSVGNIMGNFHPHGDSSIYDAMVRMSQNWKNREILVEMH
                     GNNGSMDGDPPAAMRYTEARLSEIAGYLLQDIEKNTVSFAWNFDDTEKEPTVLPAAFP
                     NLLVNGSSGISAGYATDIPPHNLSEVIDAVVYMIDHPKASLEKLMEFLPGPDFPTGGI
                     IQGADEIKKAYETGKGRVVVRSRTEIEELKGGKQQIIVTEIPYEVNKAVLVKKIDDVR
                     VNNKVPGIVEVRDESDRTGLRIAIELKKDADSQTILNYLLKYTDLQVNYNFNMVAIDH
                     FTPRQVGLQKILSSYISHRKDIIIERSKFDKAKAEKRLHIVEGLIRVLSILDEIIALI
                     RSSDNKTDAKENLKVSYDFSEEQAEAIVTLQLYRLTNTDIVTLQNEENDLRDLITTLS
                     AIIGDEATMYNVMKRELREVKKKFANPRLSELQAESQIIEIDTASLIAEEETFVSVTR
                     GGYLKRTSPRSFNASSLEEVGKRDDDELIFVKQAKTTEHLLLFTTLGNVIYRPIHELT
                     DLRWKDIGEHLSQTISNFATEEEILYADIVTSFDQGLYVAVTQNGFIKRFDRKELSPW
                     RTYKSKSTKYVKLKDDKDRVVTLSPVIMEDLLLVTKNGYALRFSSQEVPIQGLKSAGV
                     KGINLKNDDSLASAFAVTSNSFFVLTQRGSLKRMAVDDIPQTSRANRGLLVLRELKTK
                     PHRVFLAGGVQSDTSAEQFDLFTDIPEEETNQQMLEVISKTGQTYEIALETLSLSERT
                     SNGSFISDTISDQEVLVARTR"
     gene            complement(37454..39403)
                     /gene="parE"
                     /locus_tag="VC122_000277"
     CDS             complement(37454..39403)
                     /gene="parE"
                     /locus_tag="VC122_000277"
                     /EC_number="5.6.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014407511.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase IV subunit B"
                     /protein_id="HEP5640232.1"
                     /translation="MTKKEITINNYNDDAIQVLEGLDAVRKRPGMYIGSTDATGLHHL
                     IWEIVDNAVDEALSGFGDDIKVVINKDGSVSVADSGRGMPTGQHAMGIPTVQVIFTIL
                     HAGGKFGQGGYKTSGGLHGVGSSVVNALSAWLEVEITRDGSVYRQRFENGGKPVTTLK
                     KVGTAPKSKSGTVVTFMPDDKIFSTIDFKFNTISERLKESAFLLKNVKMSLTDLRGDD
                     PIIEEFHYENGVQDFVEYLNEDKETLTPVIYMEGQDQDFQVEVALQYNDGFSDNILSF
                     VNNVRTKDGGSHETGLKSAITKAMNDYARKTNLLKEKDKNLEGSDYREGLSAVLSILV
                     PEQHLQFEGQTKDKLGSPLARPIVESIVSEKLTFFLLENGEVASHLVRKAIKARDARE
                     AARKARDDSRNGKKNKKDKGLLSGKLTPAQSKNAKKNELYLVEGDSAGGSAKQGRDRK
                     FQAILPLRGKVLNTEKAKMADILKNEEINTMVYTIGAGVGADFNLEDINYDKIIIMTD
                     ADTDGAHIQTLLLTFFYRYMRPLVEAGHVYIALPPLYKMSKGKGKTEKVSYAWTDGEL
                     EDLRKEFGKGAILQRYKGLGEMNANQLWETTMDPETRTLIRVTIDDLARAERRVSVLM
                     GDKAAPRRQWIEDNVKFTLEENTVF"
     gene            39538..40179
                     /gene="plsY"
                     /locus_tag="VC122_000278"
     CDS             39538..40179
                     /gene="plsY"
                     /locus_tag="VC122_000278"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009881256.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerol-3-phosphate 1-O-acyltransferase PlsY"
                     /protein_id="HEP5640233.1"
                     /translation="MKLLLFITIAYLLGSIPTGLWIGQYFYHINLREHGSGNTGTTNT
                     FRILGVKAGTATLAIDMFKGTLSILLPIIFGMTSISSIAIGFFAVLGHTFPIFANFKG
                     GKAVATSAGVLLGFAPLYLFFLASIFVLVLYLFSMISLASVVSAIVGVLSVLTFPAIH
                     FLLPNYDYFLTFIVILLAFIIIIRHKDNISRIKHHTENLIPWGLNLSKQVPKK"
     gene            complement(40237..41505)
                     /locus_tag="VC122_000279"
     CDS             complement(40237..41505)
                     /locus_tag="VC122_000279"
                     /EC_number="3.5.2.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010922183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroorotase"
                     /protein_id="HEP5640234.1"
                     /translation="MLLIKNGRVMDPKSQRDQVADVLIDGKQIVKIASAIECQEAQVI
                     DASGLIVAPGLVDIHVHFREPGQTHKEDIHTGALAAASGGVTTVVMMANTNPVISDVE
                     TLQEVLASAAKEKIHIYTNASVTQAFNGKDVTDFKALLEAGAVSFSDDGIPLESSKVL
                     KEAFDLANANQTFISLHEEDPQLNGVLGFNEGIAEEHFHFCGATGVAEYSMIARDVMI
                     AYDRQAHVHIQHLSKAESVQVVAFAQQLGAKVTAEVSPQHFSTTEDLLLIVGTSAKMN
                     PPLRTQRDRLAVIEGLKSGVITVIATDHAPHHKDEKAVDDMTKAPSGMTGLETSLSLG
                     LTHLVEPGHLTLMSLLEKMTLNPALLYGFDAGYLAENGPADLVIFADKQERLITENFA
                     SKASNSPFIGNKLKGVVKYTIADGEVVYPN"
     gene            complement(41629..42282)
                     /locus_tag="VC122_000280"
     CDS             complement(41629..42282)
                     /locus_tag="VC122_000280"
                     /EC_number="3.2.2.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012767071.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="uracil-DNA glycosylase"
                     /protein_id="HEP5640235.1"
                     /translation="MAHSIWHEKIKSFLPEHYYGRINHFLDEAYASGLVYPPRENVFK
                     ALQVTPLEETKVLILGQDPYHGPKQAQGLSFSVPEEISAPPSLINILKELADDIGPRD
                     HHDLSTWASQGVLLLNACLTVPAGQANGHAGLIWEPFTDAVIKVLNEKDSPVVFILWG
                     AYARKKKAFITNPKHHIIESPHPSPLSSYRGFFGSKPFSRTNAILEKEGMTGIDWLQ"
     gene            complement(42415..43065)
                     /locus_tag="VC122_000281"
     CDS             complement(42415..43065)
                     /locus_tag="VC122_000281"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003057640.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter permease"
                     /protein_id="HEP5640236.1"
                     /translation="MINIPLMKDSLGFVLSGLPYTLGISLLSFLTGLVFGLLLALLGR
                     SHQPLLHYLVRAYISIMRGVPMIVVLFVLYFGLPYYGLELPALLCAYLGFSMVSAAYI
                     SEVFRSSIEAIDKGQWEAAKALGLPYALMVKKIILPQAFRIAVPPLGNVIIDMVKSSS
                     LAAMITVPDIFQNAKIIGGREWDYMSMYILVAFIYWIIAFLLERYQEFLENKLALV"
     gene            complement(43094..43957)
                     /locus_tag="VC122_000282"
     CDS             complement(43094..43957)
                     /locus_tag="VC122_000282"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003061079.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transporter substrate-binding domain-containing
                     protein"
                     /protein_id="HEP5640237.1"
                     /translation="MKKSVTLLSIGLASLLLAACAPHKSQKSSWETIKEKGVLKVATP
                     GTYQPTSFYNDNNELVGYEVDMVKEIGKRLNIKVKFVETGFDQAFTSVDSGRVDISLN
                     NFDITPKRQKKYNISTPYKYGVGGMIVRADGSSNMAKKDLSDWKGKKAAGASGTEYMK
                     VAQKQGAELVTYDNVTGDVYLNDVANGRTDFIPNDYPAQKLFVDYMLSQNPNLNVKMS
                     DAQYNPTEQGIVMNKKDDSLKKKIDAVIKDMIKDGSLKKISETYYAGQDLTKPFGKDK
                     KIPIIDTKDVN"
     gene            complement(44075..45529)
                     /locus_tag="VC122_000283"
     CDS             complement(44075..45529)
                     /locus_tag="VC122_000283"
                     /EC_number="3.5.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014407508.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidase"
                     /protein_id="HEP5640238.1"
                     /translation="MTYQDATAMAIAVQTGQTTPLELVTQAIYKAKKLNPTLNAITSE
                     RFEAALEEAKQRDFSGKLFAGVPIFLKDLGQELKGDLSTSGSKLFKNHHATKTDLFVK
                     RLEDLGFIILGRSNTPEFGFKNISDSQLHGPVSLPHDNTRNAGGSSGGAAALVSSGIS
                     ALATASDGGGSIRIPASFNGLIGLKPSRGRMPVGPGSYRSWQGASVHFALTKSVRDTR
                     NLLYYLQMEQMESPFPLATLTKNSIYQSLQRPLTIAFYQRLSDGSPVSLDTEKALRQA
                     VTWLREQGHQLVELEEFPVNMTEVIRHYYIMNSVETAAMFADIEDTFGRPMTKDDMET
                     MTWAIYQSGKDIPAWRYSQVLQKWDTYSATMASFHETYDLLLTFTTNTPAPKHGELVP
                     DSKLMANLAQAEIFSSEEQFNLVETMFGKSLAINPYTALPNLTGQPAISLPTYETKEG
                     LPMGIQLIAAKGREDLLLGIAEQFEAAGLLKIPQ"
     gene            complement(45726..46355)
                     /locus_tag="VC122_000284"
     CDS             complement(45726..46355)
                     /locus_tag="VC122_000284"
                     /EC_number="2.4.2.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010922180.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="orotate phosphoribosyltransferase"
                     /protein_id="HEP5640239.1"
                     /translation="MTLGSQIATQLLDIKAVYLKPEDPFTWASGIKSPIYTDNRVTLS
                     YPKTRDLIENGFVETIRAHFPEVEVIAGTATAGIPHGAIIADKMTLPFAYIRSKPKDH
                     GAGNQIEGRVLKGQKMVIIEDLISTGGSVLDAAAAASREGADVLGVVAIFTYELPKAS
                     QNFKEAGIKLITLSNYTELIAVAKLQGYITNDGLHLLKKFKEDQVNWQQ"
     gene            complement(46413..47105)
                     /gene="pyrF"
                     /locus_tag="VC122_000285"
     CDS             complement(46413..47105)
                     /gene="pyrF"
                     /locus_tag="VC122_000285"
                     /EC_number="4.1.1.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002984921.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="orotidine-5'-phosphate decarboxylase"
                     /protein_id="HEP5640240.1"
                     /translation="MKEERPIIALDFSSFEETKAFLDLFPAEEKLYVKIGMELYYAQG
                     PDIVRSIKSLGHNVFLDLKLHDIPNTVRAAMAVLKELDIDMATVHAAGGVEMLKAARE
                     GLGQGPTLIAVTQLTSTSEDQMRGDQNIQTSLLESVLHYSKGAAKAQLDGVVCSAQEV
                     EAIKAVTPTGFTCLTPGIRPKGSNIGDQKRVMTPNQARRIGSDYIVVGRPITQAKDPV
                     AAYQAIKAEWAG"
     gene            complement(47382..47918)
                     /locus_tag="VC122_000286"
     CDS             complement(47382..47918)
                     /locus_tag="VC122_000286"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002990141.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEP5640241.1"
                     /translation="MNVTEQNSHQILIQKLLVSIHYLTLFRDELKLVERTPSILGGEF
                     PAHLVQSELGDIVAAIDTLDMQQRLIESTFWYEESAFKLMNKTLDIVDNWIKGVDHLI
                     DLCQSKEVFQIIIGDKRIRVFGVLSDVFSSLKVSALSLKEAPIPDVLYEHIQRVNLEE
                     EAFIKYYQSPKVPTGEFE"
     gene            48140..49045
                     /locus_tag="VC122_000287"
     CDS             48140..49045
                     /locus_tag="VC122_000287"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011284709.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HEP5640242.1"
                     /translation="MRIQQLHYIIKIVECGSMNEAAKQLFITQPSLSNAVKDLEMEMG
                     ITIFNRNPKGITLTKDGVEFLSYARQIIEQTSLLEDRYKNHNTGRELFSVSSQHYAFV
                     VNAFVSLLKRTDMTRYELFLRETRTWEIIDDVKNFRSEIGVLFINDYNRDVLTKLFDD
                     NHLTASPLFKAQPHIFVSKSNPLATKSLLNMDDLRDFPYLSYDQGIHNSFYFSEEMMS
                     QMPHSKSIVVSDRATLFNLMIGLDGYTVASGILNSNLNGDQIVAIPLDVPDEIDIVFI
                     KHEKANLSKMGERFIEYLLEEVTFD"
     gene            complement(49124..49912)
                     /locus_tag="VC122_000288"
     CDS             complement(49124..49912)
                     /locus_tag="VC122_000288"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002984926.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LytR family transcriptional regulator"
                     /protein_id="HEP5640243.1"
                     /translation="MTNNQTLDILLDVYAYNHAFRIAKALPNIPKTALYLLEMLKERR
                     ELNLAFLAEHAAENRIIEDQYHCSLWLNQSLEDEQIANYILDLEVKVKNGAIIDFVRS
                     VSPILYRLFLRLITSEIPNFKAYIFDTKNDQYDTWHFQAMLESDHEVFKAYLSQKQSR
                     NVTTKSLADMLTLTSLPQEIKDLVFLLRHFEKAVRNPLAHLIKPFDEEELHRTTHFSS
                     QAFLENIITLATFSGVIYRREPFYFDDMNAIIKTELGLWRQSIV"
     gene            complement(49905..50618)
                     /gene="deoD"
                     /locus_tag="VC122_000289"
     CDS             complement(49905..50618)
                     /gene="deoD"
                     /locus_tag="VC122_000289"
                     /EC_number="2.4.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002984928.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="purine-nucleoside phosphorylase"
                     /protein_id="HEP5640244.1"
                     /translation="MSIHISAKKGDIADKILLPGDPLRAKFIAENFLEDAVCFNEVRN
                     MFGYTGTYKGRRVSVMGTGMGMPSISIYARELIVDYGVKTLIRVGTAGAIDPEVHVRE
                     LVLAQAAATNSNIIRNDFPEFDFPQIADFGLLDKAYHIAREMGVTTHVGNVLSSDVFY
                     TNMPERNMALGKLGVKAIEMEAAALYYLAAQHHVKALGIMTISDNLNDPTEDTTAEER
                     QTTFTDMMKVALETLIAND"
     gene            complement(51203..52012)
                     /locus_tag="VC122_000290"
     CDS             complement(51203..52012)
                     /locus_tag="VC122_000290"
                     /EC_number="2.4.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015647060.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="purine-nucleoside phosphorylase"
                     /protein_id="HEP5640245.1"
                     /translation="MSLMTKINETKDFLVAKGIEAPEFGLILGSGLGELAEEVENPVV
                     IDYADIPNWGKSTVVGHAGKLVYGDLAGRKVLALQGRFHFYEGNPLEVVTFPVRVMKA
                     LGCEGVLVTNAAGGIGYGPGTLMAITDHINMTGNNPLIGENLDEFGPRFPDMSDAYTK
                     VYRNKAHEVAEKMNIKLEDGVYMGLTGPTYETPAEIRAFKVLGADAVGMSTVPEVIVA
                     AHSGLKVLGISAITNFAAGFQSELNHEEVVEVTEHIKEDFKGLVKAILAEL"
     gene            complement(51996..52436)
                     /gene="arsC"
                     /locus_tag="VC122_000291"
     CDS             complement(51996..52436)
                     /gene="arsC"
                     /locus_tag="VC122_000291"
                     /EC_number="1.20.4.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010922177.1"
                     /note="This arsenate reductase requires both glutathione
                     and glutaredoxin to convert arsenate to arsenite, after
                     which the efflux transporter formed by ArsA and ArsB can
                     extrude the arsenite from the cell, providing resistance;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arsenate reductase (glutaredoxin)"
                     /protein_id="HEP5640246.1"
                     /translation="MEEITIYHNPNCGTSRNVLAMIRHAGIEPTIIEYLQTPPNRETL
                     IELLQSMGISARELLRTNVPEFEAHGLANQAVAEKDVINAMLTDPILINRPIVVTRKG
                     VKLCRPSETLLDILPVPLPSPYIKEDGEIVNPIEVKGEKDVIDD"
     gene            complement(52452..53663)
                     /locus_tag="VC122_000292"
     CDS             complement(52452..53663)
                     /locus_tag="VC122_000292"
                     /EC_number="5.4.2.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011184417.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphopentomutase"
                     /protein_id="HEP5640247.1"
                     /translation="MSKFNRIHLVVLDSVGIGAAPDADKFFNAGVADTDSDTLGHISE
                     TAGLSVPNMAKIGLGNISRPIPLKTVPTEDNPTGYVTKLEEVSLGKDTMTGHWEIMGL
                     NITEPFDTFWNGFPEEILTKIEEFSGRKIIREANKPYSGTAVIDDFGSRQMETGELIV
                     YTSADPVLQIAAHEDIIPVEELYKICEYARSITLERPALLGRIIARPYVGEPGNFTRT
                     ANRHDYAVSPFQDTVLNKLADAGVPTYAVGKINDIFNGSGITNDMGHNKSNSHGIDTL
                     IKTLQLPEFTKGFSFTNLVDFDANFGHRRDPEGYRDCLHEFDNRLPEIIANMKEDDLL
                     LITADHGNDPTYAGTDHTREYIPLLAYSASFTGNGLIPQGHFADISATVAENFGVDIA
                     MIGESFLGHLK"
     gene            complement(53740..54423)
                     /gene="rpiA"
                     /locus_tag="VC122_000293"
     CDS             complement(53740..54423)
                     /gene="rpiA"
                     /locus_tag="VC122_000293"
                     /EC_number="5.3.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014623011.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribose-5-phosphate isomerase RpiA"
                     /protein_id="HEP5640248.1"
                     /translation="MEALKKIAGVTAAQYVTDGMTIGLGTGSTAYYFVEEIGRRVKQE
                     GLQVVGVTTSSVTSKQAEVLGIPLKSIDDIDSIDLTVDGADEVDKDFNGIKGGGAALL
                     MEKIVATPTKEYIWVVDASKMVEHLGAFKLPVEVVQYGADRLFRVFEKAGYKPSFRMK
                     GDSRLVTDMQNYIIDLDLGCIKDPVAFGHLLDGTVGVVEHGLFNGMVDKVIVASKDGV
                     TVLEVPKAS"
     gene            54801..56900
                     /locus_tag="VC122_000294"
     CDS             54801..56900
                     /locus_tag="VC122_000294"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010922175.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent Clp protease ATP-binding subunit"
                     /protein_id="HEP5640249.1"
                     /translation="MTHFSGKDPFVNMDDIFNQLMANMGGYRSENPRYLVNGREITPE
                     EFQHYRQTGQLPVATTKATTSQMLTPKADSVLTQLGTNLTQEARQGHLDPVIGRNKEI
                     QDTAEILARRTKNNPVLVGDAGVGKTAVIEGLAQAIVNGDVPAAIKNKEIVSIDISSL
                     EAGTQYRGSFEETIQNLIQEVKEAGNIILFFDEIHQIVGAGATSSDSGSKGLADILKP
                     ALSRGELTLIGATTQDEYRNTILKNAALARRFNEVKVNAPSAEDTFHILMGIRNLYEQ
                     HHHITLPDNVLKAAVDYSIQYIPQRSLPDKAIDLLDMTAAHLAAQHPVTDLKTLETEI
                     AKQKERQEKAVAKEDFEKALAAKTRIETLQKQIEQHNQSQNVTATVNDIAESVERLTG
                     IPVSNMGTNDLERLKGISSRLKSHVIGQDEAVAAVARAIRRNRAGFDDGNRPIGSFLF
                     VGPTGVGKTELAKQLALDLFGSKDAIIRLDMSEYNDRTAVSKLIGTTAGYVGYDDNNN
                     TLTERVRRNPYAIVLLDEIEKADPQIITLLLQVLDDGRLTDGQGNTINFKNTVIIATS
                     NAGFGQQDTETSGFNIMDRIAPYFRPEFLNRFNSIIKFNHLQKENLEEIVDLMLAEVN
                     QTTAKKGISLTIADDAKAHLIDLGYNHAMGARPLRRIIEQEIRDRITDYYLDHPEVKK
                     LQAILEEGQLVIRQKDQ"
     gene            complement(56958..57701)
                     /locus_tag="VC122_000295"
     CDS             complement(56958..57701)
                     /locus_tag="VC122_000295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002984942.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEP5640250.1"
                     /translation="MTQVAQGRSKGKSLLCHQCNALFVFIIFLILLAVLFGVRYRNSS
                     IEGIWRTTSIDQKLGDDFAKRLTGLHQSPLIDDSLLTSSQMILTVKNNNVDLSFSVQV
                     ERDIFVKRLAAYHQNELLKTLKENHLVVGDLSSKERQIIENSMPASHELEMILDQAFE
                     KLASQIGGKYNQKTGHLSAVVLKGKVNRILHTIDIKEEVAAGHTSFSKGLLTPNGYFD
                     YTRFGKKLELLGDEKIIFKKALKKSPSSV"
     gene            complement(57849..58448)
                     /locus_tag="VC122_000296"
     CDS             complement(57849..58448)
                     /locus_tag="VC122_000296"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011054379.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YihA family ribosome biogenesis GTP-binding
                     protein"
                     /protein_id="HEP5640251.1"
                     /translation="MAEEQVLNTHNASILLSAANKSHYPKDDLPEIALAGRSNVGKSS
                     FINTILGRKNLARTSSKPGKTQLLNFFNIDDKLRFVDVPGYGYAKVSKSERAKWGKMI
                     EEYLTSRDNLRAVVSLVDLRHAPSKEDIQMYDFLKYYDIPVIVVATKADKIPRGKWNK
                     HESVVKKALNFDNSDTFIVFSSVERIGIDDSWDAILEQV"
     gene            complement(58458..59687)
                     /gene="clpX"
                     /locus_tag="VC122_000297"
     CDS             complement(58458..59687)
                     /gene="clpX"
                     /locus_tag="VC122_000297"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002984948.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent Clp protease ATP-binding subunit
                     ClpX"
                     /protein_id="HEP5640252.1"
                     /translation="MAGSRTNDIKVYCSFCGKSQDDVKKIIAGNNVFICNECVALSQE
                     IIKEELAEEVLADLTEVPKPKELLDVLNQYVVGQDRAKRALSVAVYNHYKRVSFTESR
                     DDDDVDLQKSNILMIGPTGSGKTFLAQTLAKSLNVPFAIADATSLTEAGYVGEDVENI
                     LLKLIQAADYNVERAERGIIYVDEIDKIAKKGENVSITRDVSGEGVQQALLKIIEGTV
                     ASVPPQGGRKHPNQEMIQIDTKNILFIVGGAFDGIEEIVKQRLGEKVIGFGQNSRKID
                     DNASYMQEIISEDIQKFGLIPEFIGRLPVVAALEQLNTSDLIQILTEPRNALVKQYQA
                     LLSYDGVELAFDKEALEAIANKAIERKTGARGLRSIIEETMLDIMFEIPSQEDVTKVR
                     ITKAAVEGKSKPVLETA"
     gene            complement(59817..59987)
                     /locus_tag="VC122_000298"
     CDS             complement(59817..59987)
                     /locus_tag="VC122_000298"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302775.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEP5640253.1"
                     /translation="MQRSIFGVFTAFLCVICVLCAIPAFKKNRYGLGSLFLLNAFTNL
                     VNSIHAFYGTLF"
     gene            complement(60007..60504)
                     /locus_tag="VC122_000299"
     CDS             complement(60007..60504)
                     /locus_tag="VC122_000299"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002984955.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrofolate reductase"
                     /protein_id="HEP5640254.1"
                     /translation="MTKEIIAIWAEDEAGLIGVAGKLPWYLPKELEHFKKTTLHQAVL
                     MGRVTFEGMNCKRLPQRQTLVMTRNRDYQVDEVLTMTSIEKVLEWYHAQDKTLYIIGG
                     NKVLEAFNGYFDRIIKTVIHHRFKGDTYRPNLDFSHFTQESQTFYARDAKNPYDFTVT
                     ALKHK"
     gene            complement(60584..61423)
                     /locus_tag="VC122_000300"
     CDS             complement(60584..61423)
                     /locus_tag="VC122_000300"
                     /EC_number="2.1.1.45"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003011065.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thymidylate synthase"
                     /protein_id="HEP5640255.1"
                     /translation="MTKADQIFKANIQKIINEGSLSEQARPKYKDGRTAHSKYITGAF
                     AEYDLAKGEFPITTLRPIPIKSAIKELFWIYQDQSNSLDVLEAKYNVHYWNEWEVDQT
                     RTIGQRYGAVVKKHDIISKILKQLAENPWNRRNVISLWDYEAFEETKGLLPCAFQIMF
                     DVRRVGEDLYLDASLTQRSNDILVAHHINAMQYVALQMMIAKHFGWKIGKFFYFVNNL
                     HIYDNQFDQAQELLKRQPVASQPKLVLNVPDGTNFFDIKPDDFELQNYDPVKPQLHFD
                     LAI"
     gene            61632..62807
                     /locus_tag="VC122_000301"
     CDS             61632..62807
                     /locus_tag="VC122_000301"
                     /EC_number="2.3.3.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002993959.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxymethylglutaryl-CoA synthase"
                     /protein_id="HEP5640256.1"
                     /translation="MTIGIDKIGFATSQYVLKLEDLALARQVDPAKFSQGLLIESFSV
                     APITEDIITLAASAADQILTDEDRSKIDMVILATESSTDQSKASAIYVHHLVGIQPFA
                     RSFEVKQACYSATAALDYAKLHVASKPDSRVLVIASDIARYGVGSPGESTQGSGSIAL
                     LVTANPRILALNEDNVAQTRDIMDFWRPNYSFTPYVDGIYSTKQYLNCLETTWQAYQK
                     RENLQLSDFAAVCFHIPFPKLALKGLNNIMDNTVPPEHREKLIEAFQASITYSKQIGN
                     IYTGSLYLGLLSLLENSKVLQSGDKIGFFSYGSGAVSEFYSGQLVAGYDKMLMTNRQA
                     LLDQRTRLSVSKYEDLFYEQVQLDDNGNANFDIYLTGKFALTAIKEHRRIYHTNDKN"
     gene            62794..64071
                     /locus_tag="VC122_000302"
     CDS             62794..64071
                     /locus_tag="VC122_000302"
                     /EC_number="1.1.1.88"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009880299.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxymethylglutaryl-CoA reductase,
                     degradative"
                     /protein_id="HEP5640257.1"
                     /translation="MTKTNLNWSGFSKKTFEERLQLIEKFKLLNAENLNQLKTDVLLP
                     IQTANQMTENVLGRLALPFSIAPDFLVNGSTYQMPFVTEEPSVVAAASFAAKLIKRSG
                     GFKAQTLNRQMIGQIVLYDIDQIDNAKAAILHKTKKLIALANKAYPSIVKRGGGARTI
                     HLEEKGEFLIFYLTVDTQEAMGANMVNTMMEALVPDLTRLSKGHCLMAILSNYATESL
                     VTTSCEIPVRLLDHDKTKSLQLAQKIELASRLAQVDPYRATTHNKGIFNGIDAVVIAT
                     GNDWRAIEAGAHAYASRNGSYQGLSQWHFDQDKQVLLGQMTLPMPIASKGGSIGLNPT
                     VSIAHDLLNQPDAKTLAQLIASVGLAQNFAALKALTSSGIQTGHMKLHAKSLALLAGA
                     TQDEIAPLVNALLADKPINLEKAHFYLSQLRQS"
     gene            complement(64301..65290)
                     /locus_tag="VC122_000303"
     CDS             complement(64301..65290)
                     /locus_tag="VC122_000303"
                     /EC_number="5.3.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002984966.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type 2 isopentenyl-diphosphate Delta-isomerase"
                     /protein_id="HEP5640258.1"
                     /translation="MTNRKDDHIKYALKYQSPYNAFDDIELIHHSLPSYDLSDIDLST
                     HFAGQDFDFPFYINAMTGGSQKGKAVNEKLAKVAAATGIVMVTGSYSAALKNPNDDSY
                     RLHEVADNLKLATNIGLDKPVALGQQTVQEMQPLFLQVHVNVMQELLMPEGERVFHTW
                     KKHLAEYASQIPVPVILKEVGFGMDVNSIKLAHDLGIQTFDISGRGGTSFAYIENQRG
                     GDRSYLNDWGQTTVQCLLNAQGLMDQVEILASGGVRHPLDMIKCFVLGARAVGLSRTV
                     LELVEKYPTERVIAIVNGWKEELKIIMCALDCKTIKELKGVDYLLYGRLQQVN"
     gene            complement(65277..66290)
                     /locus_tag="VC122_000304"
     CDS             complement(65277..66290)
                     /locus_tag="VC122_000304"
                     /EC_number="2.7.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014635411.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphomevalonate kinase"
                     /protein_id="HEP5640259.1"
                     /translation="MSNYCVQTGGKLYLTGEYAILTPGQKALIHFIPLMMTAEISPAA
                     HIQLASDMFSHKAGMTPDASYALIQATVKTFADYLGQSIDQLEPFSLIITGKMERDGK
                     KFGIGSSGSVTLLTLKALSAYYQITLTPELLFKLAAYTLLKQGDNGSMGDIACIAYQT
                     LVAYTSFDREQVSNWLQTMPLKKLLVKDWGYHIQVIQPALPCDFLVGWTKIPAISRQM
                     IQQVTASITPAFLRTSYQLTQSAMVALQEGHKEELKKSLAGASHLLKELHSAIYHPKL
                     VTLVAACQKQDAVAKSSGSGGGDCGIALAFNQNARDTLISKWQEADIALLYQERWEEN
                     DQP"
     gene            complement(66283..67227)
                     /gene="mvaD"
                     /locus_tag="VC122_000305"
     CDS             complement(66283..67227)
                     /gene="mvaD"
                     /locus_tag="VC122_000305"
                     /EC_number="4.1.1.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011888980.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diphosphomevalonate decarboxylase"
                     /protein_id="HEP5640260.1"
                     /translation="MDPNVITVTSYANIAIIKYWGKENQAKMIPSTSSISLTLENMFT
                     TTSVSFLPDTATSDQFYINGVLQNDEEHTKISTIIDQFRQPGQAFVKMETQNNMPTAA
                     GLSSSSSGLSALVKACDQLFDTQLDQKALAQKAKFASGSSSRSFFGPVAAWDKDSGAI
                     YKVETDLKMAMIMLVLNAAKKPISSREGMKLCRDTSTTFDEWVEQSAIDYQHMLTYLK
                     TNNFEKVGQLTEANALAMHATTKTANPPFSYLTKESYQAMEAVKELRQEGFACYFTMD
                     AGPNVKVLCLEKDLAQLAERLGKNYRIIVSKTKDLPDV"
     gene            complement(67209..68087)
                     /gene="mvk"
                     /locus_tag="VC122_000306"
     CDS             complement(67209..68087)
                     /gene="mvk"
                     /locus_tag="VC122_000306"
                     /EC_number="2.7.1.36"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011054375.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mevalonate kinase"
                     /protein_id="HEP5640261.1"
                     /translation="MNENIGYGKAHSKIILIGEHAVVYGYPAIALPLTDIEVVCHIFP
                     ADKPLVFDFYDTLSTAIYAALDYLQRLQEPIAYEIVSQVPQKRGMGSSAAVSIAAIRA
                     VFSYCQEPLSDDLLEILVNKAEIIAHTNPSGLDAKTCLSDHAIKFIRNIGFETIEIAL
                     NGYLVIADTGIHGHTREAVNKVAQFEETNLPYLAKLGALTQALERAINQKNKVAIGQL
                     MTQAHSALKAIGVSISKADQLVEAALRAGALGAKMTGGGLGGCMIALADTKDMAEKIS
                     HRLKEEGAVNTWIQML"
     gene            complement(68380..69612)
                     /locus_tag="VC122_000307"
     CDS             complement(68380..69612)
                     /locus_tag="VC122_000307"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011888981.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing histidine kinase"
                     /protein_id="HEP5640262.1"
                     /translation="MFNRIRIRFIMIASIAIFIILSSIVGIINTARCYQSQQEINRIL
                     HLISSNKGKLPGTTESSKRLGTKLSEDSLSQFRYYSVIFNANGHLLSSNTANISALDR
                     EEAQYFARLFAKSGEEKGSYRHQDSVYSYLITQLPNEEKLVVILDTTFYFRSVGDLLA
                     VSVMLAFGGFIFFVVLVSLFSGMVIKPFVQNYEKQRRFITNAGHELKTPLAIISANNE
                     LVELMTGESEWTKSTSDQVKRLTGLINQMITLARLEEQPDVVLHMVDFSAIAQDAAED
                     FKSLVLKDGKRFDLTIQPNIMIKAEEKSLFELVTILVDNANKYCDPKGLVKVSLTTIG
                     RRRKRAKLEVSNTYLEGKSIDYSRFFERFYREDESHNSKEKGYGIGLSMAESMVKLFK
                     GTITVNYKNDAIVFTVVI"
     gene            complement(69621..70289)
                     /locus_tag="VC122_000308"
     CDS             complement(69621..70289)
                     /locus_tag="VC122_000308"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002984985.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HEP5640263.1"
                     /translation="MKILLAEDEWQMSNVLTTAMTHQGYDVDVVFNGQEAIDKAKDNA
                     YDIMILDIMMPIKSGIEALKEIRASGNCSHIIMLTAMAEINDRVTGLDAGADDYLTKP
                     FSLKELLARLRSMERRVESFTPQVLQFAGVTLNINEQELSAGNAIRLASKEGKLMAFL
                     MLNQGKYLDTKTLYQHVWSDQEDYDTSIVWVYISYLRQKLLAIQANVIIAGDKDTSYC
                     LLEK"
     gene            complement(70292..70942)
                     /locus_tag="VC122_000309"
     CDS             complement(70292..70942)
                     /locus_tag="VC122_000309"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002984988.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GTP pyrophosphokinase family protein"
                     /protein_id="HEP5640264.1"
                     /translation="MKERLEHYSTSSIYSGFEVYLPLVLQTITDVIIAENIKSKKETG
                     FKLYEHFTSRIKSEASMIEKCQRKQLPLTSKSALKIIKDSIGIRIICGFIDDIYRMVD
                     LLKSIPGMSVNTEKDYILNAKPNGYRSYHLILELETHFPDILGEKKGCYFIEVQLRTI
                     AQDSWASLEHQMKYKHQVANAEMITRELKRCADELASCDVTMQTIRQLIQETTEEE"
BASE COUNT        21506 a        15037 c        12545 g        22045 t
ORIGIN      
        1 atataacagt ttcacgtttt accgaccttg acataaaaaa acacatcatt ttaaaacctc
       61 tttcacacct tcaaacaagt aacaaagcga taaaaataag cttagcaaaa ctgctaaagc
      121 ttatttttat gattctattt gttcagcgat ggtttcccat tcttccataa gagtttcttg
      181 ttcttgatcc aattggtccc attccttttg ccaagcgatt aactgcgccg tatcattgga
      241 ggcatgcatg tcttcttgaa tagcaccaat gcgctcttca atggtttcaa gtcttgcttc
      301 aatctcctca tatcggcggg tcaagcgacg acgctctttt tgattagctt tttgtagctg
      361 ataatcagtg gcagaggctt cttttgtttc ctcaactgtc tctcctgctg ctaggcgggc
      421 tagttcttct agctctgcct ttttctcaag gtaataatca tagtcaccta agtaaagggt
      481 tgagccattt tcggtaattt ccagaacctt agttgccaaa cgattaatga aatagcggtc
      541 gtggctgaca aaaagcaagg taccgtcaaa gtcaatgagg gcattctcta gcacttcttt
      601 actgtcaatg tctaggtggt tagtcggctc gtccagaaca agaaaattat tgttttccat
      661 agataacttc gcaagaagca gacgggcttt ttctccacct gacagcatag ccaccgattt
      721 tttgacatca tctccagaaa agagaaaagc ccctaatcgg tttctgatat cgacttctgg
      781 cgtagttgaa aaatcctgcc ataattcttc caaaactgtg ttgctagacg tcaaatgtga
      841 ttgggtttgg tcatagtaac ctatttcaac attggcaccg tatttcactt ctcctttgat
      901 gaaaggaatc tgtccaataa cagatttaat cagagtagat ttaccaatac catttggacc
      961 aacaatagca atcgcatcta atttgtgaac gtctaggtta ataggctcag ataaaacatg
     1021 ttggttataa ccaatagcag ctttttcaac cgtcaacacc acattgccag acggcttatc
     1081 tgcctgaaaa gtcatgtgag ctgattttct agcactagtc ggtttgtcta agcgttctat
     1141 tttttccaat tgcttacgtc tcgcttgagc acgttttgta gttgaagccc tgacaatatt
     1201 tttttgaaca aaatcttcta acttggcaat ttctttttgc tgtttgtcaa actgcttttc
     1261 ttcggctact aatttttcag cttttaaggt cataaaacga gaataattgc caacgtagcg
     1321 atccaatcca tgaggagtta aatccaaggt cactgttgcc accttatcca aaaaataacg
     1381 gtcgtgactc acaatgatta gagctccttg ataattggcc aagtagttct ctaaccaggc
     1441 aatggtttca atatctaagt ggtttgttgg ctcatcaagg accaatagtt ctggcttttc
     1501 taaaagcatt ttagccaagg ccagacgagt gttttgaccc cctgacagct cagcgatagt
     1561 catctgccac atgctttcat caaatttaaa gccatttaaa atggctttaa tctcagcttc
     1621 atatgtaaag ccaccttgct gacgaaaacg ttctgctaaa agatcataat ccatcatcag
     1681 ccgtgtcaag tcctgacctg aaacggttgc catatccatt tccatctgac ggagacgctt
     1741 ttcatcttgg cgtaatgctt cgaaaacctt gagcatttcc tcatatatcg ttttgtcaga
     1801 ttcaaaacgg ctattttgag ccagataaga caaggttagg tcttttttag tattaacttc
     1861 tccgctcgtt ggggtttctt ccccaaccaa tagcttgagt aaggttgact taccagctcc
     1921 atttggcccc actaatgcaa ttctgtctct ttcatcaact tgcagtgata tgttttgaaa
     1981 aagaacatct cctgagaaag agcgttctaa cttgtttcct tgtaaaataa tcatactttc
     2041 attctaacaa aaaaatgccc ttttgaggac attttcagcc tagtttccaa aagaaatcga
     2101 actcttttta agtcgttgat taccttcttt agcgctaaca aggaaagtgg aatgactatt
     2161 accactccca tgacgcgtct ttgcagatgg cttttgtgtt aagatatcaa aaatttgcac
     2221 cacttgctca gtaactatcg agatcttatt ggctaaaacg gaatcactgt gattagaata
     2281 gtacatcagc cctataataa taatcgttac taataagata gtttgtaaaa accctttgat
     2341 aagatgccaa aacattttca ctattagctt aggcagccac aatatgatcg ccagtaattg
     2401 tttcatttgt cacctcatgt tgtactagga aatggttaag acaacctttc ttctttttgg
     2461 tagtttatcg gtaaccactc taacaggact tctaattgct ggttccaata gtaccactca
     2521 tgtttaccat gacctttatg ataatcaatg tcaagacctt gagcctgaaa atcagcaata
     2581 gctttttcat tagtggcaaa aagaaaatcc tcatagccac accaagcata aaatttggtt
     2641 tttccatcac tttctgccac catattttta aggtaatgct tgtcaaggtc agggtcatca
     2701 aactggccaa aaacgccctg ccaataggct aattctccca ggtttccctc aagcaaggtc
     2761 tcaggagaaa aatccaaagc gcctgaaaac gatgccgcat aagaaaaccg attgcttttg
     2821 agtgcccact taaaggcacc atagccaccc atagagagcc cagcaacaaa ggttttttct
     2881 cgtttctggg tcatattagg aaagaaagct gctaagacct gcggtaactc ttgtgacaac
     2941 gcccgataat agttaagtcc ataagccgtg tctgtatacc aacctaggtc tgtcgaaggc
     3001 ataacgacta tcaaattggt atgccgcaac aagcgttcaa tagctgttcg tttttgccaa
     3061 gagttttcat tgcctcccat accatgtaac aaatataaaa caggaatatc cttatcccct
     3121 tgatcttttt tagggatttc agactggtca ggataaatca catttacctt acgctccatt
     3181 cctaaaacta ccgaatggta ttcaattgca atacttgcca ttcattacac tcctttatac
     3241 caaaataatc caatcacgta aatctactgt atttttgggt gaacagtttc ctggtttacc
     3301 agttgttaaa atcaaagaag gtaggcagtt tttgcctaac cttcctattt tcactaacga
     3361 acttccatca caaccggcag aatcgctggg cgccttttgg tttgttcaaa taagaattta
     3421 gacaaatcat ctctgacatt cccttttaat tcgccccagt caaaggtatc tttttttaag
     3481 taattgccaa cagttgtatt aactaattct gcactttctc ttaaaatatc atgacttttc
     3541 ttgacataca caaatccacg cgtattaact ttagccttag aaataatgcg tttttctttt
     3601 ttagaaacag taatagcaac aatgaaaata ccatcttctg acaagacttt acggtctcgt
     3661 aaaacgatat tacccacatc accgatagca ttaccgtcaa tcatgacatc actggcaggt
     3721 acacctcctt catggagaaa accttcgtca ttaagcacca tgatatcgcc acgcttcaga
     3781 atatgaatat tctctggaaa aataccaact tcttcagcta gctttgcatg ggcagctaaa
     3841 tcacgatatt ctccttgcac tggaaaaaga tactggggtt ttaacaaatt catcaatagt
     3901 tgcaaatcac gcccattagc atggcctgac acgcgtaagt tttgagtgat taatttaaca
     3961 gaaccaccgg ccttgtaaat caagttttct acacgcgcca ccatagcttc tttagccgta
     4021 cttggtgtag ttacaatata aacaaggtct ccttctttga tttgaacata acggtgacgt
     4081 cccgctgcca ttttttgtaa gctattaatt ggctcaccca tacgaccagc ttcaaggatg
     4141 attaattcat ggtcttcgaa tttagacata tccttaggtt taattaaaag acgctcatcc
     4201 gtaatcatta atttttctag acgtaaagct gttctgacaa tattttctgc atctgttccg
     4261 gttaaaacaa cacgacgccc atgggcagtg gcggaatcaa atacttgctg aatccgaaca
     4321 agatttgagg ctactgcagc tacaatgaca cggccatcag catcagagat aacactgtcc
     4381 atttcttcac caacttcaga ttcgctggca atctgatcgt tgcttgtcgc attgactgaa
     4441 tctgataaaa gagctagaac cccttctttc ccaatctcag ctaaacgtag caaatccgtc
     4501 tgataacctt ctcttgctgc ttgatcaaat ttgaagtcac cggtataaac aatgtttcct
     4561 ttatctgttc caataacaat acccatactt tctgggattg aatgcgttgt tctaaagaac
     4621 gaaactaggc catctttaaa ttcaatttca gtatcactgt ctaccacatg aaaattatta
     4681 aatttcttag tgctgttatt acttttcaca aatagcttgg ctaattcaat agtcaattca
     4741 gatccaaata caggagctga tacttcagct aacaaataag gaagggctcc aatggcgtca
     4801 gcatggccat gactcaagaa aatcccttgt actttacctt tgttttcaat tacataatct
     4861 aaattgggga tgactaaatc taccccaagt tgctcatttt cagggtactt gagccctgcg
     4921 tctaaaataa acatagaatc gttaatttct accaaataga agttttttcc gtattcacgg
     4981 acccctccca aggcaatcat tttaatatca gtcatgatac tcctttaaat attttattat
     5041 tacaaacggt ccttacaacg taagtcgaat tactgttatg tagctatgct actatcttct
     5101 atagtatatc atatttgagg cgttttttct ctaaatagcc ttgatacttt cagattattt
     5161 tcagtatgga aatcaaaaaa tcactgactt attattagtc aatgatttag atcgttgatt
     5221 agtcaaatag ttggtaatag tcagtaatcg tcagttgttc tttttccatt tgatttaaat
     5281 ctttaataat gtttccatct ttcattacaa tcagacgatt cccataagtt aatgcatctt
     5341 ccatatggtg ggtaatcatc aaagctgtta agccatcttt tgtcacaaac tcatctgtca
     5401 attgcattaa agattgactc gtcttagggt caagggcagc cgtatgttca tctaataata
     5461 agagagctgg ctttttcaac gttgccatta ataaacttaa agcctgtctt tgtccaccag
     5521 acaaaagacc tgctggtgtt tctaaatgct tttctaggcc atttcctgtt cgtttaacca
     5581 aatcctcaaa actagctaag tgctctgtta tttttcgaga agccaaggag cgtttttctc
     5641 ctctttgacg tgcgattaag agattttcag ccactgtcat ccgaggggct gttcccattt
     5701 tgggatcttg aaagactcgc gataggtaaa gagctctttt ttctgccggc caatgcgtca
     5761 catcctgacc aagaatccta atttgccccc gtgtcaaact tagcgttcct gcaattacat
     5821 taaaaagggt tgattttcca gctccatttc ctcctaaaat agttaaaaaa tcatgttcat
     5881 agatagttag tgtcacattg tccagtattg ttttagcatc ttcaaaacca ttatctacgt
     5941 caactgttgc attgatcaat tcaataattt ttgtcatcgt gttaacctca ctcccttaaa
     6001 gtagcgctct tttaccactg gtatcatgag gcacagtgct aaaacaagag cactaattaa
     6061 tttcaaataa cttgtattaa aacctagcgt aataactact gaaattaaaa attgataaag
     6121 aatcgagcct attacaatag caatcaaacg ttctaaaaga gtcagtccag ttgagtatag
     6181 tacttcacca acaattatgc tcgccaaacc aataacaata actccaatcc cttttgagac
     6241 atctgcatag ccatcttgtt ggctcactag agcacctgat aaagctatta aactattaga
     6301 aaccacaagt cccataactt ccatccaatc cgtgttaata ccaaaactct tagccatatc
     6361 tttattgtct cccgtagcga tataagcttg acctagatta gtgtaaagga aatagatcaa
     6421 aacgctaata acaatcacaa cagttatcag acctgttaaa aggctattta aatcaattga
     6481 aaaaggtaaa caatcctgaa tacgtttatg atcatgtaaa cccaagtttg ctcttcccat
     6541 taccattagc ataatggagt tacatgacgt catcaccaaa attccagcta aaagtgttgg
     6601 catttttcct ttcgtatata acagccctgt taaaaaacct gctaaagcac ctgataacat
     6661 accaagtaag gttgatagaa atggattcca ccctaagcta atagcggtga ccgcaacagc
     6721 tcctcctaaa ggaaaagaac cttctgtagt catgtcaggg aagtttaaaa tcctaaaggt
     6781 caggtagatt cctaatccta aaactcccca tataaggcct tgtgaaactg atgaaataat
     6841 catatttttc ctcatattta tattagcggg taactctcgc cagtataaac cctttcttta
     6901 cattaatttt tagatttgtt ttatacttag ttcagcaccg tggcttagtc ttttttccaa
     6961 tcactttaac gaaactaaga cagaaaacaa aaaaatcgag aatcatgttt aaacttactc
     7021 aaaaggcaag attttttacg tgtctgaaat agagcatttt aaaactaaaa atgataaaaa
     7081 taacccttac atcctcaaga cgattgtctg acttggcatc caaattcatc tcagtgaggg
     7141 tgttttttat ggttttttct tgacctttta tatcacatgt cattgtatta attctcaata
     7201 aaagaagctg aaggaaaatc ttccctgtta atagcaatac ctaattctgc tgctacattc
     7261 ttattaatta gcggttgacc aatatccaca actttgacag gaacatctat aacacgcttt
     7321 cctttgatta attttaacac ctgattagct gtttgaactc ccaaatcgta ctgattttga
     7381 gaaatggctg ccaaaccacc tttttcaacc attcgatcaa cgctagtgta gacaggaatc
     7441 tttgcagcct tactggttgt catgactgat gaaaatgcac ttgctatagt attgtcttgg
     7501 gggataaata ccgcgtctac tttacctaac ataactgaca tcgtggcagg aacttcgtta
     7561 gtagaaggaa ccgcgtatga aataacttga tagccctttt tgcgagctaa ccgcctaaac
     7621 tctttaactt gagataagga attatcctca ttacttgcat ataaaattcc aactgttttg
     7681 acatgtggtg tcagattttt cattaaatca attgtctgtt taacaggcac tttattagat
     7741 agaccagtta cattagcttc aggttgatcc aattgcatca ctaatctaga tcctactgga
     7801 tcagatactg ctgacatgac aactgggata tccttgctgg cagctgctaa gccttgcgcc
     7861 gccggggttg cgataccaat cacgatatca cttcctgatt gcactaattg cctactcatt
     7921 gtttggattt tactctgatc cccctcagca ttcataacct taatcactac attttgtttt
     7981 tgaggcatct gctttttgag ttgatcttca atacctcgct taatatcatc aagagcctca
     8041 tgagtaacga attgtaagat accaattgta atgttttgtc ggttagccaa atcacggttt
     8101 tcctttgata ctccttttga taatagagag cctatcacta ttacagttaa aacaaggagg
     8161 gtagctatta aactcttgtt tttcatctga attcctcaca tttcatgtca atctctttaa
     8221 aacgttgatg atgagaatac ttctcaaaag aaaaagcact tagtaatgat tactaggcgc
     8281 ccttgtgaaa tccaatgtca gcgcatagat atctttttag aagtactcta aaagtcgtat
     8341 ctatgccaat tcataaacac aacttatcta tgccagcctt gccaatttac taagtgttgt
     8401 gtttttacca tcattgactt cctttctcgt ttttttaaca ttataaccta aatcaaaaaa
     8461 attgcaagct ctttcgtcca tatttttagt caagtatttg tggtataatg aagagattag
     8521 aattcagaaa ggagaaacca tgtcttttga cggctttttc ttacaccatt taaccaacga
     8581 attaaaagag aacttgcttt acggtcgtat ccaaaaggtc aatcaaccct ttgaacggga
     8641 acttgtctta accattcgta accatcgcaa gaattataaa ttgctattgt ctgcccatcc
     8701 tgtttttgga agggtacaaa tcacccaagc agactttcaa aatcctcagg tacctaatac
     8761 ctttaccatg attatgcgta aatacctcca gggtgcagtc attgagcagt tagaacagat
     8821 tgataatgac cgcattatcg aaatcaaggt atctaataaa aatgaaatcg gagatgctat
     8881 tcaggcaacc ctaatcattg aaatcatggg gaagcacagc aatatcatct tggtggaccg
     8941 tgcagaaaat aaaatcatcg aatccattaa acatgttggc ttttcacaaa actcttaccg
     9001 caccatttta cctggctcta cctacattga gccccctaaa acagctgctg ttaatccttt
     9061 tacaattaca gatgttcctc tatttgaaat cctccaaaca caagaactaa cagttaaaag
     9121 cttgcaacag cattttcaag gtttggggcg agacaccgca aaagagttgg ccgagcttct
     9181 gaccacagac aaattaaaac ggttccgtga gttttttgct agacctactc aggcaaatct
     9241 gaccacagct tcttttgcgc ctgttctttt ctcagatagc catgcgacgt ttgagacttt
     9301 atcagagatg cttgatcact tttatcaaga taaggctgag cgtgaccgta tcaatcagca
     9361 agccagtgat ttgattcacc gtgtgcaaac tgaactggac aaaaatcgta acaagctaag
     9421 taagcaagaa gctgagctgc tagccactga aaatgctgaa ttattccgtc aaaaaggaga
     9481 attattgacc acctacctca gtctggtccc aaacaaccaa gactctgtga ttttagacaa
     9541 ttattataca ggggaaaaaa ttgagattgc cttagacaag gctctgacac caaatcaaaa
     9601 tgctcaacgt tattttaaaa aataccaaaa gctaaaagaa gctgtcaaac acttatctgg
     9661 attgattgcc gataccaagc aaagtattac ttactttgag agcgtggact acaatttatc
     9721 tcaagcaagt atcgacgaca tcgaagatat tcgcgaagaa ttatatcagg ctggcttttt
     9781 gaaaagtcgc caaagagaca aacgccataa acgtaaaaag cctgagcagt acctggcttc
     9841 agatggaaaa accattctca tggtgggacg caataacttg caaaatgaag aactcacctt
     9901 taaaatggct aaaaaaggag aactctggtt ccatgctaag gatatccctg gaagtcatgt
     9961 catcatcaaa gacaatcttg acccaagtga cgaggttaaa actgatgctg ccgaactagc
    10021 tgcttattat tcgaaagcta ggttatcaaa tctcgttcag gtcgatatga ttgaagctaa
    10081 aaaattacac aaacctagtg gcgccaagcc aggatttgtg acttatactg gtcaaaaaac
    10141 tttacgggtt actcctgacc aagccaaaat tctctctatg aaattatcct gataagcaac
    10201 taaaaaacag ctctcctagg aaagttattt cccctaggag aactgttttt ttattctgat
    10261 tgttggtgct tgatatggtc aagaaagtct tgcatattca catcatcagg cacttgtctg
    10321 aggtaagcgg tagccatttg gatagccttg tgaaactggc caaactccct taataaatag
    10381 gcataatcct gtaaaaactc cggattttct gccaaatcag ccgaaatctc attataaaga
    10441 gccaaggcaa cttcttcttg ttccaaggcg tggtaagcct ttgcaatggt ccatttggtt
    10501 aagacattat caatcgtctc acgattaaga gcaataacct cctcaaaacg ctcggcatca
    10561 aaataaaggg tcactaaacg catgagaatc tcttcgtcat caacagctac ttcctttgct
    10621 tgaagcaggt aattttcagc attctgccta tcgtgtaatt cataagaaag ctgcgatgct
    10681 agtaataata attgactgtc aaatgcattt ttacgtaggc cctgttgaac caagcgcaaa
    10741 gcttctgacg tcttatgctc ttcatgaaga ctcaaagcat aaccatattc ataacctgga
    10801 taatctggat tgatggtttc caactgttta aagtaaagat tagctttttg ataattttct
    10861 tggtcataca agagggtcgc caattcaaaa acagtttcat cttcgtattc aatggccacg
    10921 gccttttcca agaattcgat ggctgcctca aatttcccta agctagcata ggctcgtcca
    10981 atgcgttgat aagtggaaat acctgtcaat tcaaggattt gacgattgtc taactgtgca
    11041 tagtaatcaa tagcttcttt aaaatactgc aaggacatgt caatttctgc caaaccaaaa
    11101 atgaccaatg gctctgggct gatgctcact gcttgaagca atttttcacg cgccacctct
    11161 gtcagtcctt ccatgtcata gaggtcagcc attactaaga gagctgacaa ataattgggg
    11221 ctatcttttg aaaccttatc caaatacaag aaagcttctt caatggcgtc gtcttctgct
    11281 gcaatctgag ctaaatttat attgagttct gggtaatcat cagctaactg caaataaata
    11341 cgcttggcat gtggcaaaaa acctatactt tctaagtatt ctcctaaggc gattaaacta
    11401 tctgcatcat cttcttttaa agctttttgg aaatattttt cagcgtgcgc taggtcttgc
    11461 tgatccaagg aagcaatcat tttttcacta tttaacatgg tccttaacct cctcaatcac
    11521 caatacttct tcgtcataca aaccgctaac ttttttgtac cagtcaaata attctttaat
    11581 cacaactttg acagaagcgt agattgggat gactaagaag acaccccaaa cgccaaacat
    11641 tgatcctgca gtcaacaata agaacatgat cgtaatcgga tgaatgctca gcttatttcc
    11701 aagaacaagc ggtgccacaa aacggccttc gatggtttgc tcaatcataa agataacaag
    11761 caccttaacc aacataaatg ggccttgtac catagccata atcacaacag gtatcatggc
    11821 aaggaaactt cctaagtaag gtatcatgtt aagaaagcca gcaataatcc caaaagtgat
    11881 ggcatatttc agtcccacaa gactaaacat gatagaaaac ataaagccga caacaattgc
    11941 aaccgtaacc tgcccttgga catagcctga taattgcttg ttaatatctc ccaagactcg
    12001 aaccataggc acccgtaatt tgagaggtaa gacattcaca aggccatttt tcatgtggct
    12061 actgtctcgt aaaaaataaa agagaataaa gggcgaaata ataatagcca cggtaatccg
    12121 agcaatggca ccagcaaaat taccagccca gtcaatagca ttttttgaaa aactttgcgc
    12181 gtaatccaaa gccttttgac ttgtatgggt aagcatgtct tggagttggg gcttgtagct
    12241 gactaaccat tcattttcaa gaagtttatt ggcttcttca ttgaccttgc ccacatattt
    12301 gggcagatcc tcaataaaac tggtaagttg ggtctgtacc atgggaacaa agcctgaaat
    12361 cgcccagaca aaaagtaagg taatcaaacc aaaaacaata aaaatagagg tggtacgatt
    12421 cgggcccaag tgattaataa ggtcaactaa gggtttggtc agataataca gaatggtcga
    12481 aataaccaag ggcaacatga tgaccgcaaa aaaagaaatc actggcataa ataaaaaaga
    12541 gattttagta aaaacaaaaa tcgttaaaaa ggctaaaagt gttattacta aagccatggt
    12601 agcttgatta tttaaaaacc atttgtaaaa taagctatct gttctagact tgtcaggtat
    12661 taagggcatt aagatagggt tcctttcttt gggaaaatga taagttttac tggtaacctg
    12721 ggtaaatcaa taaaaggtca cagatttatc catgagttgg ttgttttggt cataggtaaa
    12781 tttggcagag aaaaaaggac ggtcttctaa aatccattta aaaatgtcat aatcaccttc
    12841 ccaagttggc ttttccaaca cttgatcgta aggtacccat tccaaggttc cttctcgtga
    12901 ctcttcatcg gaaatgaggt ctccttcaaa tcctgttact ttaaaaacat aggtatacca
    12961 gtcatgacca ggggtaaatt ctggaaaagt aatgattcct ttaaaggcca tctctgtaac
    13021 agtcaaatgg gtttcctcta aaatctcacg acgggcacac tcatctgggg tctcaccagc
    13081 ctcgagctta cccccaactg aaatccactt gcctttgtgg acatcatttt cttttttatt
    13141 gcggtgtaat aatagcaaag aatcgccatt atcaatgtaa caaatggttg ctaattgagt
    13201 catctatctc tttcctttcc atctttgttt ctattatacc aatctctaga aaatatgcta
    13261 tcattttatg agtcaaagtg tgaatactta gtcacgctca aaaccttcta aagatgcctt
    13321 aaaaaagtgt agataagagc ctacaatgaa aaacatcaat ttaaactatc aaaagaacct
    13381 tgtcatatca acgataattg atatgacaag gttctttaat gttttattta atcacttctt
    13441 gagttttagc atacttggct tctacagcat ctttttctgc tttccaccat gtctcatttt
    13501 ctgtatacca cttaatagtt tcttccaaac cttctgaaaa gtttgtaaat tgtggttccc
    13561 agccgagttc ttcacgcaat tttgtagaat caatagcgta acgaagatcg tgaccagcac
    13621 ggtcagttac gtgatcataa gcgtcttttg gttgacccat tttctcaagg ataagctcaa
    13681 gaacttcctt gttgtttttc tcgccgtcgg caccaataag gtatgtttca ccgatacgac
    13741 ccttagtcaa aatagcccat actcctgtag aatgatcatt agtatgaatc cagtcacgga
    13801 catttttccc ttcaccgtat agttttggct tgatgcctga taaaatattg gtgatttggc
    13861 gaggaataaa tttctcaatg tgctggtaag gtccgtagtt attggaacaa tttgaaatgg
    13921 tcgctttcac accgaaggaa cgtacccatg ccttaacgat aaggtcagaa gctgccttag
    13981 ttgatgagta aggtgatgat ggattgtatt ttgtttcagc agtgaatttt tcacctggtc
    14041 cttcaccctg tcctggaagg tcttcacgaa gtggaagatc tccataaact tcatcagttg
    14101 acacgtggtg gaaacggata tcgtatttac gagctgcttc aagcaaagtg taagttccga
    14161 taaagtttgt atggataaat ggacttggat cttccaatga gttatcgttg tgactctcag
    14221 ccgcatagtg aacaatagca tccgttttgg cagccaattt gtctaccaat tcagcgtcag
    14281 cgatatcacc aacaactaac tcaacacgat caccaagaat agcttcaatg ttagcacggt
    14341 tacctgcata tgtcaattta tcaaggacag ttacatgaac atctgggtgg ttattgtaga
    14401 catagtgcac aaagttagat ccgatgaaac cagctccacc agttacgata atatttttat
    14461 acataagata taaagggacc tttcgtttcc gaaaaaatct attaaaagta ataactctaa
    14521 caaagacgtt ttaggcctaa gaaaacatcg ctttctaaga tttgagtccc atgttcagct
    14581 caaagagaga aactgaactt tcctttcatc atttttagtt gttacttgtt acctgaagta
    14641 gagaagaata actcccctca tatccacctc aaatgattaa tcccaatcat ctgagtatgt
    14701 tgtttcccta taggtctttt ggtttcagtg gtttgacatc actaagaaga ggatggtttt
    14761 tatctgcttc agaaacttct gcagctgcta gattttccca agtaatccca agacttgggt
    14821 cagcatagtt tacaaaagca tacttaggtt tcaagtcaag agcccagtag tcattgacaa
    14881 gataactgta agagacagtc tctgaaagaa cttgaaagcc attagcaacc cctctaggaa
    14941 caaacattcc ttttgaagca tcaatcactg tctggtagac gtttccaaaa gtctctccct
    15001 cacgaagatc aacccaagct cctaaaacct tgccatcatc agcaactgag atgtatttat
    15061 cccaaggttc agcatgaagt ccacgcagca catgctgacg tgaaaaagaa acgttgtttt
    15121 gtagttttcc ttcctcaaaa aaacgttctg gaaagccaat tggcaacatt ttttcttttt
    15181 ggaaattttc tttaaaccag ccacgattat ccccacgaac aggaatatca aactctagta
    15241 agcctgggat ttctttgatt tctcggcaag ccaatggttt gtcaaaaaaa gtttctgtca
    15301 ttaagcttct ccaatcaaac gaagcaagta ttgcccgtat tcgtttttct ttaaagactg
    15361 cgccaattca tggacatctt ctttactgat gtaacccatg cgataggcaa tttcttccag
    15421 atttgccact tgagcgtttt gcaaacgttg aactgtttca atatattgag ccgcttcaag
    15481 tagactttca tgcgttccag tgtctagcca tgcaaaacca cgccccatca attcaacgga
    15541 gaggtcacca cgttccaagt aagccttgtt gacgtctgta atttccaact caccacgcgc
    15601 gcttggttta atgtttttag caatttctac cacgtcattg tcatagaaat aaagtccagt
    15661 cactgcaaag tgggacttag gcacttctgg tttttcttcg atagagatag cattcatgtt
    15721 ctcatcaaat tccaccactc caaagcgctc agggtctttc acttgataac caaaaacggt
    15781 ggctcctttt tctttggcag ctgccttttg aagcatttta gtgaggccat taccgtggta
    15841 aatattatct cctaaaataa gagcaacacg gtcatcaccg ataaactcct ctccaataat
    15901 gaaagcttgt gctaaaccat ctgggcttgg ttgttcctta taggataagc taatgccaaa
    15961 ttctgaccca tctcctagca attcttcgaa acgaggaaga tcttgggggg ttgaaatgat
    16021 taaaatatct ttaatccctg ccaacatcaa ggttgacaat gggtaataaa tcattggttt
    16081 gtcataaatg ggcatcaact gtttagatgc ggcacgggtc agtggataca agcgtgtccc
    16141 tgaacctcct gcaagaataa tacctttcat acgtagtgct cctattcttc tatataccct
    16201 atcattctat cattttttga aagcagtgtc acgctttacc agtagaccgc aaaaggtaag
    16261 gctcagggga atagtcgcta ggcgttaaaa gtccttcgtg tcctagcaat aattgagcta
    16321 attcatagcc aatcaaaggg ccaactgtta atcccgatga ccctaaacca ctagctgtat
    16381 aaaggttctt caatcctgga acctgaccat aaaaaggaga ataatcactg gtataagctc
    16441 ttatccctac acgacatgat ttttgagtag cttcttttaa taacggcaaa taagtgagag
    16501 cttgcatttc aagtttttct aagacctgcc aatcaggctc caaatcataa cctttatcat
    16561 tttcatggct cgcaccaaca gaaatcttac cttgattgaa aggaatgagg tctatttcac
    16621 cttctggcat aacaacggga taattgtcgc tgatgatgtg atgaacgtca taatccaaga
    16681 gttgtccctt ttgtggtcgc acatcaacct gatagcctaa aggcatcaat aagtcaggta
    16741 gccatgctcc tgctgccaga atgacctgat caaaataatg accagcaatc tcataaccac
    16801 ttccttgctg tttcagagtg actttttggc gaataactgg ataaccacta gtcttgagta
    16861 acgtctcaca taaagcggct ccctccactc tagcagctcc tgaagcgtac agacagttat
    16921 caaagccaat caatccttta aaatcatccc ccagttcttt acgattttta atggctaact
    16981 caccaataat cggtgattct acctttcttg ctaacgctaa ttcataaaga tctcttagtt
    17041 tttcttcctg cttttttaaa acatagatgc cattttgctg ataaaaatct gttgcaaaac
    17101 catcttcttt gaggtcattg attaattgct gataaaagtc cgctcctaaa cgagccatgc
    17161 ggtaccaaac tttattgcga cgctttgaaa accaaggact aataatacca gcagcagcct
    17221 tagtggcttg tccttgacca tggtcaaaaa tggtaacttc tttttgacca ctttgttgta
    17281 aataataagc ggcggttgat cctacaatcc ccgccccaat aatggctatt ttcattcctg
    17341 gtatcctttt atcttacttt aagaccttaa cagcctataa atggctaaat ggattggtcg
    17401 aggctttgct tgaaataatg ctgacatccc aaccgttctc ttctttccac ccttggagtt
    17461 tctcctttaa tttctccgta aaaagcacct caatatgatg ccccgggtca accgcaaata
    17521 gtccttctgt cagcatctcc tgtgctgtat ggtaatagat atcccctgta atataaacgt
    17581 ctgcgccttt ttgcacagca tcctgataaa attcaccacc acttccgcca cagatagcaa
    17641 tctttgaaat caaagggttt tccttatcgt aacggataag tcgcacggta tctaaatcaa
    17701 acaccctttt gaccttactt gctaactctt ctaacgcttg ttctttaact gttccaatac
    17761 gaccaatgcc aaagccttct ttggtttcag acaagtaagt tgcttccttg atttctagca
    17821 ggtcacaaaa ccaatcattt agcccgcctg gtacaatgtc aatattggta tggctcacat
    17881 agacagaaat gtcatgcttg accaaatcta gaaggatatc acgctgaggg gatgaaacca
    17941 gatctttaag cggtttaaag atgggagcat gcttagttat aatgagatca acttcatttt
    18001 tgatggcttc tgctactgtt gactctcgga tatctaaagt aatcataacc ttacgaatgt
    18061 ctttatccaa tgaacccatt tgaagacctt taacgtctct ttccatagat aaatcaagtg
    18121 ggcaaaaggc ttcataggca tcaattaggg ttttagcttt catgaacaat cacctctttt
    18181 atttgttgta tttttgtcaa gagaagctga cgctcttgag ttttttcttc tgggatgcag
    18241 gacaaggcat aagctaactt atccatttct cgttgccact tctctttaaa aacgactgat
    18301 ttttcctgag aaaggtatgg accaaaacgc aattctgtcg cggataaggc cttttcacca
    18361 tgctcagcaa cgatgatttc ataatatttg tcattttcag ccatgatggt ttctgcaaca
    18421 attttaaaag cgttaaccga taaccaagcc cgtaaatcat cctcacgatt gttgggctgt
    18481 aaaaccagac gttcaatgcc ctgcaatttt tccttacctg cctctaaaat atcagtaatc
    18541 aaacggccac ccatgccaca aatggtaatg gctgtcacat catcagcttc ttcaaaggct
    18601 gcaaggccat tggctaagcg cacctgaata tgctcggcaa ggccagactg tgtgacattt
    18661 ttaagagcag attcgtaggg acccctaacc acttcgccag caatggctgc tgaaatctga
    18721 ttagtttcta ccaaaaatat gggaagatag gcatgatcac tgccaacatc taataatttg
    18781 acaccttttg gaacataggc cgcaacttgg gctaaacggt ttgataattg actatccatc
    18841 ttgatttctt tctaaaacgt aatggttcta ttatagcaaa attacctcca taaaaaaaga
    18901 aaaccaaagg ctaggctttc gattttccat aagtattttg atagtactta cagtaagact
    18961 gaacgggaca aatctcacat tttggttttt tggctaaaca atggtagcgt ccaaagaaaa
    19021 tcaatcgatg gtgagtaata atccaatctt tctttggaat tttagccatc aaatctgctt
    19081 caatctgctt gacatcggca tctggtgatg aaatattgag cctctttgag actctagcca
    19141 catgagtatc aaccgcaata gctggaacac cataaacctc tgccaatacc acattggctg
    19201 tttttcggcc aacacccggt aaactttcaa gctctttgtg ggtttttggc acttgaccct
    19261 taaaatcatc acgaatagcc tgagcagttt taatgatatt tttggcctta tttttataga
    19321 gtccaatcgt tctcagagca ttttcaacat cagaaagttc agcaaaagct aagtcttcta
    19381 tttctggata agactgccat agtccaggag ttaccttatt gacggcctta tctgtcgttt
    19441 gagctgataa aatcaccgca attaacaatt gaaaaggtgt ttcccagtct aactctccct
    19501 tagcctcagg aaacatttga ccaatgatgg tcaggacttt tgctaatctt gcttttccaa
    19561 ttctcatgaa tcactccata aattcatcgc agatagaaaa tcctctgata tcgtcacttg
    19621 agaaagatct tcgccttctc taacacgtct acgttcttct acttgtcgaa gattaacaat
    19681 accttctttt cgccaattac gtaagatagc ctggatatat ttccaatttg ttttgccgtt
    19741 gaagacagct tcttttaaag cttctcgtat caaatcagga tctgttttat catcacgaag
    19801 cgttttttca agatcttcta actcaaaagg actcaagaat cgtcctaatt cacgttcaaa
    19861 ttcatcaact aatcgtttga aatggtttgg tgtttcttgt ttgtcaattt cagttgccgt
    19921 ttgactgaca aacaattgat ctaacttggc taacacagga ctcgcatcaa aaataatttc
    19981 aatttctcca gtaagttcaa ttgtccgcat atctaaaagc ccttgactag tcaaagatga
    20041 aatgatttta ttaatatcag caactgattt tccaagcgca tgagcaattt gactgggcgc
    20101 taagtcgtct cgctttgtcg tattttgaag atagaaaaac tgccagacca aaaaatcatc
    20161 tgagctctta aaaagatctt tgtaatgaaa aagcaaggca cttgggataa ccaaattacc
    20221 tgacttataa tgttctaaaa aactcataat ccctcaatca aaaggctaaa atcgaatcta
    20281 tagcctttac tactttaaat cccctaatca gatgctgatt aatgtgctta cccaggaaac
    20341 tgcattaaga ctttatagtc atagttccta atagcatgac gaccatttaa accttctaat
    20401 tcaataagga aggcacaacc agctacaata ccaccaagtt tttcaatcat ttcaatagtt
    20461 gcttttaccg taccgccagt cgctagtaaa tcatcgacaa tcaaaacacg ttgtccaggc
    20521 ttaatagcat ctgcatgcat agttaaggta tcaagaccat attctttttc gtaatcggca
    20581 gagacaacat ctcttggcag ttttcctggt ttacgaacgg gagcaaaacc aattcctaac
    20641 tcaactgcaa caggacaacc aatgataaaa ccacgcgctt caggtccaac aaccatatca
    20701 atatctttat cacaagcgta ctgtgctatt tcacgaatag catagctata tgctttaccg
    20761 tcagccatta aaggtgagat atctcgaaac gtaataccag ctttagggta atctttgatc
    20821 gatgcaatgt aatttgttaa atccatagtt aattctttct agtggcaaat tgccaaatat
    20881 aaaatactct tcattatatc acgaaacata tttttcgtaa aataagactt ctgacattat
    20941 gtttatctga tacttcattc gtaactacct caataactac tgtcaactgc tttacttatt
    21001 gatcgtttga tttcaccaaa taatcataca tctctttcgg gctagccaaa gccatgattt
    21061 cttgaaattt caccaattct tttaagtctt ggtaaatgtg gctctctgaa atctctcgtt
    21121 tttgcgcttg ggggttaacc gtcatcagcc catcgtcaat cgtcacaaaa gacaactctt
    21181 caaaaatttg aatcaactta atcagtaaaa gtttttctat gtttaaatag tgactcaact
    21241 ctgtcaattt atgccgtaaa tcaaattcag gaaactgata gatggttttg tagagcttgg
    21301 caaactgctc tctgctgcca aatcctgtta aataataagg atgtttcatc tgatttttaa
    21361 aatagatggc gtcaaaatcc ttgtgaacaa actggttgcg ccaggttttg aaatcttcag
    21421 ggatgtcatt aatcagaatg actctggcat ttgggtcttc ttcaatagtc ggaatacctt
    21481 caggaacttt ggctgttttt gtccttaagt caagaagttg cactccgtca acacgagcat
    21541 ctactaacat gaattggaga ctcgtgttcc cattccaatg gttgacagat aaggtaactg
    21601 ctaattctaa accagtagct tgtcggaact cttgcagttg acttccttga ccaaaagcca
    21661 gtacgtcaaa ggaagctttg ccctgactga ctttaaattt aagatggctc tggtcttgcc
    21721 ctatggtacg cgcttgacta acccttatgt ccttgacata aaagaccggc ttttgatgat
    21781 ccatcccata aggggctaat ttatctaaac tctttaaaat atcaaggcta aggtcgtcca
    21841 aagacagtct ttcatcaata gttaacgtat ttttggcagt ctgatctaag ccacgttcta
    21901 tcacaaactg acaaagaaag tctgaaagag cttccaagtt gtcaacgggc agggtcattc
    21961 ctgctgcacc agcatgtccc ccaaaggctg taaataactc acgcttgcca ttcaaggctt
    22021 caaaaatatt aatagcttct aaacttctgg ccgagccttt tgcaaagcca ttatcaatgg
    22081 tcaaaacgac gaccgtttgt ccaatagttt ccatgatacg accagcgaca atcccaagaa
    22141 caccaggatg ccagccagct tgtgccaaga cttgaacagg tttttgagga tcaaccatag
    22201 ccatcgcttg atcaaaaatg tcttggacta aggccttacg ctcttcattt tttttcttaa
    22261 tcatcaaggc aatcgcttga gcttcttggt catcaaagcc cgtcagtagc tcaatggctg
    22321 gattagggtc atctaagcgc cctaaagcat ttaattgcgg agcaatctga aagccaatag
    22381 cgtcttcatt aaaatgttcc aaatcaatag gagacaaaga catcaactcc tgcaagccca
    22441 ctctttcggt gtgtttgagc atggccagac catttttgac taaaacacgg ttttcacctg
    22501 tcaagcttac catatcggca atggttccaa tagctaccaa gtctagacag tctgttggta
    22561 aactttctag caaagctgtt gccaatttaa aggcaacgcc acagccagct aagtgtttaa
    22621 agggataatc agcatcagga tgctcaggat gaatgatggc aaaggcttct ggcaattctt
    22681 caggcaggct atgatggtca gttacaatga catcaacttc ctgttcttga gcatatctaa
    22741 tcgcttcatg tccggccacg ccattatcaa ccgtgataat tagggacaca gcttcctgct
    22801 caataaaata tttatagacg ctctggttgg ggccatagcc atctgtaaag cggttgggaa
    22861 gataaaccaa aggctctgct cccatcatgt ctagggtttc tttgacaata gaagccgacg
    22921 tcatgccatc agcatcatag tcgccgtaaa tgagaattcg ttcgccctct tcaatagctt
    22981 gacgaattct tggcacagct tttgccatgt catgtagcaa gtaaggatca tgcagttgag
    23041 acaagtccgc tgttaaaaac tcatctaagg cttcttcagt ccttatccct cgctcataaa
    23101 tcaactgagc ggcggtctgg gttagacctt ttgtctttgc tagcttaaag aagccatcat
    23161 ctggcttctt gtcttttatt ttccagctat atttggattt aatcatttat aattaaatac
    23221 ctttcttgca agataatcag ataatttagg gaaaagggtg taaaattgat gggtcaccgc
    23281 taaactaaag ggcaaattca attctcgttt atttttcccg ataatagaaa ccaaacgctt
    23341 agccacttga tttggttgga gagtaaatgt accaacgctt tccaaataat gtccagacgg
    23401 gtcagcttgg tcaaaaaact tggtggcaat gggacctgga ttcacggtgg tcacgtaaac
    23461 ccctttatcc gctaattcta agcgaagggc attggaaaat ccgataaggg caaacttggt
    23521 ggctgaataa atgctcgatt tggctgacgc aatcaagcct gccatggaca caatattaat
    23581 aaggtgacct tgcccctgct ctgccatttt ctgaccaatc aagcaagcaa agtgaatgct
    23641 cgctagggtg ttaacctgaa acatatcagc tatttcttgg gcagaaaact cttcaaagcc
    23701 tttgaaagct ccgtagccag cattattaat caagacatca atacggccat agcgctggta
    23761 aatctgggcg accattttct caatggctac tggattggta atatccaact caaggcattc
    23821 tttgttgtca atatgctgat aacagtgttc taggcgttct ttgttacgtc ctaataaaat
    23881 caagctgtct tccttgggta actgcttaac aattgcctga gccagtcctc cagaagctcc
    23941 cgtgataaca atgattcttt gtgccattaa ataatcactt cctctaaatc ttgtaccatt
    24001 ttaacatttt caaatatagt ggcggcatct ttttccatct ggcgacaatc acgccctaaa
    24061 aagcgagctg atacatgatt gagcaagagg cgttttgcgc ctgcttcatg agcgatttgt
    24121 gctgcctgca tgttggtaga atgcccgtga tttctcgcaa tccgctcgtc acccttacca
    24181 taggtggatt catgaaccaa aacatctgca tctttagcga gtttaacact ggcagacgtt
    24241 ttgcgcgtat cgccgataat ggtgatgatt ttgccttttt taggggcaga aatataatct
    24301 ttggcacaaa tcaggcggcc gtcttccaac tcaacatctt gaccattttt aattttgcca
    24361 aagagtggtc cgaagggaac acccgctgct tttaaggctt ctgcgtctag ggttccttct
    24421 aagtcttttt gaactactcg ataacccatg caaaaaatgg tatgcgccag acgctctgca
    24481 tagaccacaa acttatctgt ttccattatt ttacctaatg atttgtcatc aaattcatga
    24541 aaatgaatct gataaggtac acgcgctcca gaaactttta gactagttaa cacgtaagtt
    24601 ttaatgccaa ttggtccata aatatcaaga tctgtctgtt cttcactagc ttgaaaagac
    24661 cgacttgata aaaatcctgg taatccaaaa atgtggtccc catgcaaatg agtaatgaaa
    24721 atctttctta tttttcgtgg cttaatcgtc gtttctaaaa tttgtctttg cgttccttca
    24781 ccgcaatcaa acatccagac ttcattgatt tcgtctagta atttcaaggc aagacttgac
    24841 acattgcgct gcttggctgg ttgaccagct ccagttccta aaaattgtaa ttccattcaa
    24901 tctattcttt ctattgggtg atgtaaatca taaattctcg ttgggcaaaa tcataatact
    24961 tttgaccaga aaatgtttgg ctaagagccc ataactcctc tacattaaac cccttaagca
    25021 tggtgatacc gtttgccaga cggtaaatct tcgacaagag tgtgtcatca ggcgtaatgt
    25081 cttcacattc tccttgctca aactgcttag cctgcatttt aagctggcct ttgtcttcat
    25141 aaaccatata ttgtgggaaa atttgtgtta attcaaagaa gagttcgggt ttcttaggct
    25201 ctgctttttc agaaaatacc aatgcaactt cttggtcatt ttggtaagca aaaccataag
    25261 cttttccaga caaattgagg cgtacaaagg gcttttcttc ctcaaaagat ggttggtcag
    25321 taaaaagccc caaagcctta gaaagattaa aataataatc agtcgcagct tgcggtgact
    25381 gtttctggta gatttcagca aagtaggcat taataacact tggtggtggt gtgataaaag
    25441 ctagtttttg ctggtctgtc aaactggcta atagatggtt taggtagttg gtatccagac
    25501 tagtaaatcc accataggtt tttgctaaat caatataatt attcataaaa atcgtcaagt
    25561 ctccattgct gttttgggga aatgtagccc gaaatatcct ctatttcttg gtcaaacgtg
    25621 tagtggtcta aaagagcaac tcggttcaaa tcatacagct tgtacgcctt gtcttgatgc
    25681 actttgattc taaaaggaac aaactggtct cgaatttggt caatgattaa acgtctcaag
    25741 agaattttgc tatcctttga acgggctgaa atcctaatat ttggaaaagc agtagctgtg
    25801 aattgttctg ctatatccac cttgttataa atagctaaac gtggaatatt aagcatgtct
    25861 agctctttta gaaggtttaa aaccactttt tcttgctcac tatgattagg gtcgctagcg
    25921 tcaatcacat gtaacagcaa atccacatat ttgctttctt ctagggttga tttaaaagca
    25981 gccactaatt ccgttggcaa gtcttgaata aagcctacgg tgtctgttaa ggttgcttga
    26041 aattgatttt ctaaatagag ttgctttgta gttgcatcta aggtagcaaa taattcattg
    26101 gcttcatagt ggctgtcatc tgtcaacaag ttcataatgg ttgacttgcc agcattggtg
    26161 tagccaatca agccaatttt aaaggtatct gaacccacac gacgatctct aattgtttga
    26221 cgatttttct ctacctgagt taattggcgt tcaatatcag caatctgatg acgaatcgaa
    26281 cggcgattta actctagttg gctttcacct ggtccacggc tcccaatacc acctgcttga
    26341 cgacttaaca tgatgccctg tccaaccaaa cgaggtaaca tatatttcaa ttgggcaagg
    26401 tgtacttgta atttgccctc atggcttctg gcacgcatgg caaaaatatc taagattaac
    26461 tgcatacggt caatgacttt tacctctaaa acagcttcta gattagcatt ttgcctagca
    26521 gtcaaccgat tgttcacaat aactgcatca atctcatcag cttccacaat cgccttaatc
    26581 tcatccagtt tccctgagcc gataaaggtt ttgctgtcgt aacgttctcg tttttggctg
    26641 aaacttgcca taactttgac gccagctgtt ttggcaagat tagctaattc tgtcatggac
    26701 atatcaaaat gttctgttgt ctgaagctca actcctaaaa gaatcactct ttcttgctga
    26761 cgctttgtct caatcatcac ctttaccaat cctccaaatt ccgtaaattc tattataaca
    26821 tatcttcaac agattctgct aattttgcgt catgacttct ctttctgacc aagaaaatcc
    26881 ctgaccctat catgtactac ctgtgggtaa tcaggagccg ttatggcagt gaagctaaca
    26941 gccattcgat tgcggaacca agttagctgt cgcttggcaa aacgtctcgt gttttgtttt
    27001 aactgatcac tagcttctgc caaagtcatt tcacctacaa aataggggaa taattcctta
    27061 taacctatgc cacgactagc ctgaacagtc gggtaatgct catataacca ttttgcttct
    27121 tccaataagc cgttttcaag catacgatcc acgcgctgat tgatacggtc atagatgact
    27181 tggcggtcat cattcaagcc gataataagc ggctcataag ccgtctcggc attctctaat
    27241 tcatcagcaa actgagccaa ttcaagggct cggatcgccc gcctgcgatt cacctgctca
    27301 atggtaatgt tattgacttg caagcgttca tacaagtcgt gatcgtccaa ttgctccaac
    27361 tcattccgat aggcttttac cgcctcttga tcaacttgac ctcctagatg ataaccttcg
    27421 agtaaacttt gtaagtaaag accagttcca cctacaataa ttggtaattt cccacgacta
    27481 acaatgtctg aaatagcttt ttgagcatct tgaacaaaat cgtaggccga ataagactcc
    27541 gtcacctcac gaatatcagt taagtgatga accgctgctt cttgctcttc ttgagtggcc
    27601 ttggctgttc caatatccaa ctgtcgataa acttgctggc tatctcctga aataatttct
    27661 ccattaaaag ccttggctaa actaatccca agtgctgttt ttccaaccgc tgtcggacca
    27721 acaatcacaa ctatttttat ttttgtcatt ttcttgtctt tctattagaa aagcgtgtcc
    27781 aaaaacgcct tttacttgaa aattagctta tttttcgcta ccatatatta taacataaga
    27841 ttaaggacca ctgaggtcct tcactagaaa tggagaataa tcatggaata tcaatttgtt
    27901 aaaggtcttg ctactggcgt cctagcaact gctgctactg ttgctggtgc tgtctttgct
    27961 gtaaaaaaaa caatcattga ccctgaagaa gaaaaagctg ctttcattga agaaaaccgc
    28021 aaaaaagctg cgcgtcgtcg tgtagctcgc taacgtgaca atggatcaat ttaaaagaga
    28081 ctagcacatt aaagtgccgg cctctttttt agtaatctcg ataactagtt agtagtaact
    28141 gtctcttgat gagccaagat gttagctgct ttacttattt tgaaatagaa atctcaggta
    28201 agccccataa acatacgtga ctaaagctgt cgccaacaat acctctccta gcaaaagaac
    28261 ctgccaacct aaaccttgcc aacctaaaaa agataaggtc atttttaagg aagtggcact
    28321 aatcacaaac ggaaaggtaa aagctgaaaa acctgggtta aagggacgat tgagtaagcg
    28381 aggaagctgt acaaccacaa aagcatataa cagttgtgag ctgatgagaa gaaagataac
    28441 cattcctact tgtggtctag gaaaggttgc taaataagac gctgaaagta aggacagagg
    28501 ggcacaaaac gttgaaatat tgggcattac tgcttgacct aaaccaatcc cataagtctt
    28561 tttggccata aaaggtaaaa tcacagcagt taagagaaga caaacccaaa aaatcacctg
    28621 tcctaataaa aactgcctac tagcaggtgc tgttaatgcc gccattgcaa ttccaacaaa
    28681 aagcacggac caggaaggaa aaacgttatc ccatgaaaaa gaaaagacaa aacgatactg
    28741 gtaataagca atcaggacaa gatttcccaa aaagaatagc caccaagtga gaaagcctaa
    28801 ccaacaccct cctgtcactt tgagaaagag tgaagacaac aacatccctg tcataaaaaa
    28861 agttggaaaa acagaggcaa ttagaggttg tcgtaactgc attttggtat cgttaaggtt
    28921 cctaaccatg ccaacaagca agataccata aataaaaaga gctgccagaa ttcccaaata
    28981 actaaaaatc gaaacataag tagccaacaa attgccaaat gacaacgttc ctaatgccaa
    29041 tcctgacatg accaatggtg ggtttttaag gtgtttcata agtgtccttt tctgcctatt
    29101 aacagctatt tttagtgaca taatcttgtt tattttatca catttcgagc atttttgcca
    29161 taaaggctgc cagatgcgga taattctcgt aagattttgg tgttttttga gaccatagcg
    29221 ccatggcgta agcataagca tctgcaatgg tttgttgatc tcgataaaca tgcccactat
    29281 ccccaataag accatctaaa tgcgtcatag caatatccat ccgcttgtaa gaagcttgtt
    29341 tgactttttc aagcgcttga ttgtcttgag aaatagtgta gcgatgaggg ttgtacattg
    29401 gcctaaaaac aaggggaaaa tcacctgtta agaaatccat gatttcgttg aatcgacttt
    29461 ctgccaaaag attatcttgg ggaatgagct gagatttcag ggtttgtttc gctataaaga
    29521 gcaagataat acctgcctgt gtgacgattt gaccatcatc aagctgtaaa gcttaaacca
    29581 ttcccaaagg attgatggta aggcagtctt cagaatccag ttttaccttc tctatctcgt
    29641 aatacagaaa ctcacaattc ttttgaaaag gttctagaca gagttggtgt tgacgtcttc
    29701 gctgttgatg gctctgcatg acaaaatgag cttggtgtcc acgttcacta gtatcttgtt
    29761 gcaaagtctt tgcaatttgg tctcatcatc tgtatcacaa aaaacaaacc agtttcctga
    29821 tttgttttga gatattaggc acctacaaat tctggcaagg attctccaat agcttctaaa
    29881 cgttctgccc atccttgacg tgataaacca ttttcttgca catagcgatt acgttcatgt
    29941 gcacgacatt caggcgagca tcctcgaaca tactttgttt cgttttcttc tgaagcaaaa
    30001 atttgtttgt tacaaaacgg atttgcacaa ttgacataac gttcacaagg tgttccatca
    30061 aaataatctt tactaatgac agttggatta acatgattaa ttggcaccga gatacgatca
    30121 tcaaaaacat acatcgcacc atcccaaagt tcaccttgaa cttccggatc ttttccatat
    30181 gttgcaatac caccgtgtaa ttgtccaaca tctttaaagc cttctcgaac catccaaccc
    30241 gagaatttct cacagcgaac accacccgta cagtaaacaa caacacgttt ttccataaac
    30301 ttatctttgt tgtcacgtac ccattgtggt aactcacgga aattgcgaat atcaggacga
    30361 attgctccac ggaagtgacc taagtcatat tcataatcat tacgcgtatc taaaacaaca
    30421 gtatcttcat caagcaaggc ttctttaaat tgtttaggat ttaaatactc acctgttgtt
    30481 tccaaaggat taatatcact atcaaagtta ttgtcttcta acccaaggtg cacgatctct
    30541 ttcttataac gaacaaacat tttacggaaa gcttgttgat tttcctcgtc aatcttgaac
    30601 caaagatcag caaaacgctc atcgctgtgg acccaatcca tatacttctg agttgtttca
    30661 taatctccag aaacagttcc attaatcccc tcatccgcaa tcaaaatacg acctttaagc
    30721 ccaatagatt tacaaaattc tagatgtttc gcagcgtact cttgcgcatt ttcaattgaa
    30781 acatatttat aatagagtaa aactctgatt ttttctgaca ttttttctcc ttcattgtta
    30841 ttcagttact tcctattata tctaggaaaa acaagtctag gcaagtcatt tgtttgtctt
    30901 ttttttcaca aaatagatct caatttattt ttgatgagat gagatgcaac ccattactcc
    30961 tttaagcaga tcattagaag ctttatttag aaaatgctaa gataaaacaa ggaggccaaa
    31021 atgacttatg aactttgttt agaatatggg acttatccgt tgtcacgtgt ttatgcttat
    31081 tggggagaag atcaaaatcc cccaacattc attcaagaag atcgactgct ttgccataaa
    31141 ctagaaacta tgaatcacct gttccatgat ttatttgtaa ctattgaatc acaattccat
    31201 tatgttggtt ttaacatgcc tgaaaaaaga gcgcagattc ggatactata tcaagaagta
    31261 gctactatat taaaatccaa atataaggat tatccaatta agattgagac gtttcttctt
    31321 taaaaatcta atactaaaaa actaactgtg cttagacaga tgacacagct agttttttag
    31381 gtaaaactct tcttaaattt taaaacgttt cgaaaaatca ctaaatgctt taatagcact
    31441 tgctaaaaat ggtttcacca tctcatcaat aattaacctt gattacttac aatcttatga
    31501 atctgagtca aataaaattc aggctgtggc attacagaga tatttaaaac ctatactcat
    31561 cgttttcaag gtcagaaaaa taatgatttt gaaaaggttc gtcaactctt ctagataatt
    31621 ttggacaaat tttggaaaag aaacaaaaaa agcctatcaa atagggcttt tttatatgat
    31681 ttctatccac cttgagggaa tcgaaccccc atctcaagaa ccggaatctt acgtgatatc
    31741 cattacacta aaggtggtca caactttgtc aataccctta tagtatatca taaaatgaaa
    31801 taatatgaaa taacttttct aaccagaaga caataaaaag ttgaggaaaa cctcaacttt
    31861 ttagatttat tacaattcaa tatcaccgaa aagatcagcc attgagaatc cagtttgtgt
    31921 ttctgggagt tcgtagtcac gtcttgattc acgttttgga cgacgtgggc gtgattgacg
    31981 tttttcttct ttgttgtctc cttcagcttg cgctgggcgt tcttcaagag ctttgattga
    32041 taatgatacg cgctcatcag ctgcattcac ttcaagaact ttaactgtta cttcttgtcc
    32101 tacagaaagt acatcttttg gattttcaac acgtttgtgt gaaatttgtg aaatatgaac
    32161 aagtccatca atacctggta atacttcaac aaaagcaccg aagtcagtca agcgttttac
    32221 tttaccttca acaacatcac cttgagcaag tttttgttca acgccgtccc atggtccagg
    32281 tgttgttgct ttaagtgaaa gtgacacacg accagcttct tcgtcaattg aaagaacttt
    32341 aacttcaact tcttcaccaa ctgaaacaac tgatttaggt gacacattac gttcgtgaga
    32401 caattcagtc acgtgaacaa gtccgtcaac accaccaagg tcaatgaaag caccaaaact
    32461 tgttaaacgc gctacagtac ctgtaacgac tgcaccttct gaaatcttag agaacacttc
    32521 agcacgagct tctttagcgg cctcttcgat aacttcacga cgtgatagga tgaaacggtt
    32581 ttctgctgca tcaacttctt tgattttagc atcaaactct tgtccaacaa atttttcagt
    32641 gttgcgaaca aaacgagtgt caatcattga agcagggata aatccacgaa gtccttcaaa
    32701 ttcaactgaa agaccacctt taacagcacg ggtaccttta acagtaacaa cttcgccttc
    32761 acgaccaaca agtttgtccc aggctttacg agcttccaaa cgttttttag atactaggaa
    32821 agtaactgta tcagtatctt tacctactac ttgacgaaga acaagtactt caactgtgtc
    32881 gccagcttta acaaagtcgt taatatcagc atcgcggtcg ttagtcaatt cacgaagtgt
    32941 caagacacct tcaacacctg ttccttcgat aacaacgttt gcttgaccgt tatcaacagt
    33001 taaaacttcc gcagtgacaa catcaccagg gttcacttcg ctaacactgt ttagcaaatc
    33061 ttcaaattca ttcattctaa aaaatcctcc aacaaaagct agccaatgct agcttttgat
    33121 aacaatatat tttctcaagg taacccgcaa cgacaacaac tcttatcttt gacggtctca
    33181 gggagcacct gataccttaa actggggtag ctggattcga accaacgcat gagggagtca
    33241 aagtcccttg ccttaccgct tggctatacc ccaataaaat tttttacaga aaactaattt
    33301 tctaattaga ttagtttccg aaatggaaag agagggattc gaacccccga acccgaagga
    33361 gcggatttac agtccgccgc gtttagcctc ttcgctatct ttccgtaaca acaaaaacta
    33421 ttctatcata gtttttgtaa gctggcaagt gtttttgtct aattttaatg gttcaaattg
    33481 tagttttcaa tgatattttc aaccgcttgt tcgagcgttt tgaagaagca atctactcca
    33541 tcatttttta agacagcaaa tttctcatca agttctgcaa tttctccaac cacacgttta
    33601 ccaatttgta attgatagcc atcaatctta gtttcattaa ccataacttt gtgatcaatc
    33661 agttggattt caattttttt atctttttta ctcatcactt tacctcactt tgtctatttt
    33721 acacaaaatc aaaaaagctt gcaagagcaa gctctatttt aatctacttt tacaatccaa
    33781 ccttctggtg cttcaatatc cccaaattga ataccgacca actcatcgta taaacggcgt
    33841 gtcacaggac caacttctgt ttcactgtaa aagacatgta agttatcctt gtactggata
    33901 ccaccaattg gagagataac agctgctgtt ccacaagctc ctgcttccac aaatttatcc
    33961 aattcattaa ttggaacatc accctcaatt actgtcatac caagtctttc ttcagctagt
    34021 tgtaagagtg aatacttcgt aatagatggc aagatagatg gactaagtgg cgtaacaaat
    34081 tcattatttg cagtaatgcc aaaaaagttt gcagctccga cttcttcaat tttagtatgg
    34141 gttgctggat ctaagtaaat cacatcagaa aagcctgctg atttagcggc cttacctggt
    34201 agcaaacttc ctgcatagtt tcctcccacc ttagcagcac ctgttccgta aggagcggcg
    34261 cggtcaaacg cttcagaaac aataaaatta gtcggagcaa gtccaccttt gaaatagttt
    34321 cctactggca tggcaaaaat ggtgaaaata tattcttcgg ctggtttaac cccaataata
    34381 tctccaactc caattaataa cggtctcagg taaagcgttg cacctgttcc gtaaggtggt
    34441 acaaagtctt cattggcttt aaccacttgc ttagctgcat caataaattg ctctgttgat
    34501 acttgtggca tcaacaagcg gtctgctgtg gcttgtaaac gcactgcatt acggtctggt
    34561 ctaaatagtt gaatagagcc atctttggtg cgataagcct ttaaaccttc aaaagcttgt
    34621 tgcccataat gaagcgctgg agcgctttct gagatatgta aggtggcatc ttctgttaat
    34681 tgacctttat cccattgacc atttttgtaa taagaaatgt aacgaaatgg caacttatga
    34741 tattcaaatc ctaggttgtc ccaatcaatt gctattgtca tcatcgtacc tcttttctgc
    34801 ttttttctat tgtaactttt tttcaataat tttcaagttt attctactct tttgcattaa
    34861 tattctaaat ttaccataaa attaaaaaga gttttcactc tttttaacgt gttctagcta
    34921 ccagtacttc ttgatccgaa atagtgtctg aaataaagga gccattgctg gttctttccg
    34981 ataacgaaag cgtttctaaa gcaatctcgt aggtttgacc agtcttacta atgacttcaa
    35041 gcatttgttg attagtttct tcttcaggta tatcagtgaa caaatcaaac tgctcagctg
    35101 aagtatctga ttgaacgcct ccggccagaa aaactctatg aggtttggtt tttaactctc
    35161 gtaggaccaa tagtccacga ttggcacgac ttgtctgagg aatatcatcg acagccatcc
    35221 gtttgagaga acccctctga gttaagacaa agaaagagtt acttgtcaca gcaaaggctg
    35281 aggcaaggct gtcatcattt ttcaagttaa tgcctttaac accagcagat ttcaaccctt
    35341 gaataggcac ttcctgactg gagaatcgca aagcataacc atttttagtc actaatagga
    35401 ggtcttccat aataactggt gacaaggtaa ccacgcggtc tttgtcatcc tttagcttca
    35461 catatttggt tgatttagat ttataagttc gccaaggact tagttcttta cgatcaaagc
    35521 gtttgataaa accattttgt gtgacagcca catacaagcc ctggtcaaag gaagtcacaa
    35581 tatcagcgta gagaatctct tcttctgtcg cgaagtttga aatggtttgt gataaatgtt
    35641 caccgatatc cttccaacgt aaatcggtca attcatggat tggccggtaa atgacattgc
    35701 ctaaagttgt aaacaacaaa aggtgttctg ttgttttggc ctgtttaacg aagatgagtt
    35761 cgtcatcatc tctcttgcca acttcttcca aactagacgc attgaaggat cgtgggctgg
    35821 ttcttttgag gtatccgccc cgtgtcacac taacaaaagt ttcttcctct gcaatcaagc
    35881 tggcagtgtc aatctcaatg atttgacttt ctgcttgtaa ctctgataaa cgaggattgg
    35941 cgaatttctt tttcacttct cttaattcac gtttcatgac gttatacatg gttgcttcgt
    36001 caccgataat agcggaaagg gtcgtgatta agtctcttaa atcattttct tcattttgga
    36061 gagtaacaat atcggtatta gttaaacgat aaagctgcaa ggttacaatc gcttctgctt
    36121 gttcctcaga gaaatcatag ctgactttca gattttcttt agcatcagtc ttattgtcag
    36181 acgatctaat caaagcaata atctcatcta agatggacaa gacacgaatc aatccttcaa
    36241 caatgtggag gcgtttttca gctttggctt tatcgaattt agaccgctca ataataatat
    36301 ctttgcggtg actgatatag cttgatagta ttttttgaag ccctacttga cgtggtgtga
    36361 agtgatcaat ggcaaccata ttgaaattat aattaacttg aaggtctgtg tatttcaaca
    36421 aataatttag aatggtttgg ctatctgcat cttttttcaa ctcaatagct atgcgaagac
    36481 ctgtgcgatc agattcatca cgtacttcta cgatgcctgg gactttattg ttaacacgaa
    36541 catcgtcaat ctttttgacc aagactgctt tgttaacttc ataaggaatc tcagtgacaa
    36601 taatttgttg tttaccacct ttaagttcct caatttccgt cctagagcga acaacgacac
    36661 gtcccttacc tgtttcgtag gcttttttga tttcgtcagc tccttggata atgcctcctg
    36721 tcgggaaatc tggcccaggc aaaaattcca tcaatttctc taagctagcc ttgggatgat
    36781 caatcatata cacaacagca tcaatcactt ctgacaaatt gtgcggtgga atatctgtcg
    36841 catagcctgc ggaaatacca gaagacccat tgacaagtaa gttaggaaaa gctgctggta
    36901 aaacggtggg ttctttttct gtgtcatcaa agttccacgc aaaagagacc gtattttttt
    36961 caatgtcttg aagaagataa cctgcaattt cagacaagcg agcttctgta taacgcatag
    37021 ccgcaggcgg atcgccatcc attgaaccat tatttccgtg catttcaaca aggatttcac
    37081 ggtttttcca attttgactc atccgtacca tggcatcata aatagaggaa tccccatgcg
    37141 ggtggaaatt ccccatgata ttaccaactg atttggctga tttacggtaa cctttttcaa
    37201 aagtattgcc atccttattc atcgaataaa gaatccgacg ctgaacgggt ttcaatccat
    37261 ctcgaatatc aggaagtgcc cgttcctgaa taatatactt ggaataacgg ccgaagcgtt
    37321 ctcccataat atcctcaagg gacatgtttt gaatgtttga catattcaaa tagctttctt
    37381 tttcattatt tattaattac ctaaaaaaca atcgtcttta gcacttgtaa ttatctgtga
    37441 gaaacttgta tgactaaaac accgtatttt cttctaaggt aaacttgacg ttatcctcga
    37501 tccactgacg gcgtggggct gctttgtcac ccatcaacac agaaacacgt cgctctgctc
    37561 ttgccaagtc gtcaatagta acacgaataa gggttctggt ttcagggtcc atagtggttt
    37621 cccataattg gttagcattc atttcaccca gtcctttata gcgttgaaga atagctcctt
    37681 taccaaattc cttacgtaaa tcttctaatt caccatctgt ccaagcgtag gacactttct
    37741 ctgttttacc tttacctttt gacattttat aaagcggggg aagcgcaata taaacgtgac
    37801 ctgcttccac caacggtcgc atataacggt aaaagaaagt cagcaaaaga gtctgaatat
    37861 gagccccatc ggtatcagca tcggtcatga taataatctt atcatagttg atatcctcga
    37921 ggttaaagtc agctcccact ccagctccaa tggtataaac catagtgtta atttcttcat
    37981 ttttcaagat gtcagccatc ttagcttttt cggtatttaa aactttgcca cgtaatggta
    38041 aaatagcctg aaatttgcgg tctcgtcctt gtttggctga acctcccgca gaatctcctt
    38101 cgaccagata caattcattt ttcttggcat ttttggactg ggctggggtt aattttcccg
    38161 acaacaagcc tttgtctttt ttgtttttct taccattacg tgagtcatca cgcgcttttc
    38221 gagcagcttc gcgagcatct ctagctttaa tggctttgcg aactagatga gaagctactt
    38281 ctccattttc gagtaaaaag aaggttaatt tttcagaaac aatgctctct acaataggtc
    38341 tcgctaaggg acttcccaat ttgtctttgg tctgtccttc aaattgcaag tgctgctcag
    38401 gaactaagat agataatact gctgagagtc cttctcgata atctgagccc tctagatttt
    38461 tatccttttc ttttaagaga ttggttttac gagcatagtc attcatcgct ttggtaatag
    38521 ctgactttag tcccgtttca tggcttccac catctttggt gcgcacattg tttacaaagg
    38581 ataagatatt gtcggagaaa ccatcgttat actgcaaagc aacctcgact tgaaagtctt
    38641 ggtcttgacc ttccatgtaa ataacaggtg tcaaggtttc cttatcttca ttgaggtatt
    38701 cgacgaaatc ttgaacccca ttttcataat gaaactcttc aataatcggg tcatctccac
    38761 gaagatctgt caagctcatc ttgacatttt tcaaaagaaa agcagattct ttcaatcgtt
    38821 ctgaaatggt attgaactta aagtcaatag ttgaaaaaat cttatcatct ggcataaagg
    38881 ttacaaccgt tcctgattta gacttaggag ctgtgccaac ttttttaagg gttgtaacag
    38941 gtttcccccc attttcaaag cgctgacgat acactgaccc gtctcttgtg atttcgactt
    39001 ctaaccaggc agacaaagca ttgacaacag aggagccaac accgtgcaaa ccacctgacg
    39061 ttttgtagcc accctgacca aatttaccac ctgcatgaag aatcgtaaaa ataacttgaa
    39121 cggtgggaat ccccatggca tgctgacctg ttggcattcc acgtccgcta tctgccacac
    39181 tgaccgagcc atctttattg ataacaacct taatatcatc tccaaaacca gacaaggcct
    39241 catcgacagc attatcaaca atttcccaaa tcaggtggtg caatcccgta gcatctgttg
    39301 aaccgatata catccctgga cgcttgcgaa cagcatctaa cccttctagg acttggatag
    39361 catcgtcatt ataattatta attgtaattt ctttcttagt caacaccgac ctccagacag
    39421 tttcatcttt tttatcttac aagtttttta ccaattttgc aaaataattt gctaattttt
    39481 tccgaattct tttccagatc tagttctcca atctatgaat atgttataat aaggattatg
    39541 aaattactac tttttattac cattgcctat ttacttggtt ctattccaac tggactatgg
    39601 attggacagt acttttacca catcaactta cgagagcatg gatcaggaaa tactggaacc
    39661 acaaatactt ttcggatttt aggtgtcaag gcaggaacag ctaccttagc tattgatatg
    39721 tttaaaggga cactttcaat attgttacca attatttttg gtatgacttc aatttcatcc
    39781 attgctatcg gctttttcgc agttttaggg catacttttc ctatttttgc caactttaaa
    39841 ggtggtaagg ccgtagcaac aagtgctggt gtattgctag gctttgctcc gttatatctc
    39901 ttttttttag catctatctt tgttttagtt ctctatttat ttagcatgat atctttagct
    39961 agtgtggttt cagctatcgt tggtgtgtta tctgttttaa catttcctgc cattcatttt
    40021 cttttaccaa attatgacta ctttttaact tttattgtga tattacttgc gttcattatt
    40081 attataagac acaaagataa cattagtcgt atcaaacatc atactgaaaa tctaatacct
    40141 tgggggctaa atttaagcaa acaagtgcct aaaaaataaa tttactaaaa gcctgacttc
    40201 ctctgaaagt caagcttttt tgttttaact catttgttaa ttagggtaaa caacctctcc
    40261 gtctgcaata gtgtatttca cgacaccttt gagtttgtta ccaataaatg gggaattact
    40321 agctttagaa gcaaaattct cagtgatcag acgttcttgc ttgtctgcaa aaataaccaa
    40381 atctgctgga ccattttctg ccaaataacc cgcatcaaaa ccataaagca aagctggatt
    40441 aagcgtcatt ttttctaata gtgacataag tgtaagatga cctggttcaa ccaaatgcgt
    40501 caaacctaat gacaaagaag tttcaagtcc tgtcatacca gatggagctt tagtcatatc
    40561 atcaacagct ttttcatcct tgtgatgagg agcatgatca gtggcaatga ctgttataac
    40621 acccgatttc aagccttcta tgacagctaa gcgatctctt tgtgtacgaa ggggaggatt
    40681 catcttggcg ctggtaccta caatcaataa gagatcttca gttgttgaaa aatgttgtgg
    40741 ggacacttca gcagttacct tagcacccaa ctgctgagca aaagccacta cttgaaccga
    40801 ttctgcttta gataaatgtt ggatatgaac atgagcttgc ctgtcataag caatcatgac
    40861 atctcttgca atcatactat attctgctac accagttgcc ccacaaaaat gaaaatgctc
    40921 ctcagcaata ccttcattaa aaccaagaac cccatttaac tgaggatctt cctcatgcaa
    40981 agaaatgaaa gtttggttgg cattagccaa atcaaaggct tcttttaaaa ctttagaact
    41041 ttctaaggga ataccatcat ctgaaaagct tactgctcct gcttccaaca aagctttaaa
    41101 atcagtcacg tcttttccat taaaggcttg ggtgacactg gcattggtgt aaatatgaat
    41161 cttttcttta gctgcgctag ctaatacttc ctgtaaagtt tcgacatctg aaatgacagg
    41221 attggtatta gccatcatga caaccgtagt cacaccacct gatgctgctg ccaaggctcc
    41281 cgtgtgaata tcttctttgt gcgtttgacc gggttctcta aaatgcacat gaatatcgac
    41341 caaacccgga gcaacaataa gaccactagc atcaatcact tgagcttctt ggcattcaat
    41401 agctgaagct atctttacaa tctgtttacc atcaatcaag acatctgcca cttgatctct
    41461 ctgcgatttc gggtccataa cgcgtccatt tttgatgagt aacatgctct ctccttcttc
    41521 tcacttatct gtaaaactga tagacagacc tgtctgccaa taatactgaa actttgtcca
    41581 taggctgctt acctcaaacc atctgcctga taatcactaa tgcttgtcct attgtaacca
    41641 atctatgcca gtcatgcctt ctttttctaa aatagcattt gttcttgaaa agggtttgct
    41701 gccaaaaaag ccacgataag acgacaaagg gcttggatga ggactctcga tgatgtggtg
    41761 ctttggatta gtaatgaagg cttttttctt ccttgcataa gctccccaca aaatgaaaac
    41821 tacagggctg tctttctcat tcagcacttt aataacagcg tctgtaaatg gctcccatat
    41881 taagcctgca tgcccattag cctggcctgc tggcactgtt aagcaagcat tcaaaagcag
    41941 aaccccttga ctagcccaag tgcttaaatc atgatggtca cgaggaccaa tgtcatctgc
    42001 taattctttt aaaatattaa taagggatgg cggagcagaa atctcttctg gaactgaaaa
    42061 tgataagccc tgggcctgtt tggggccatg ataagggtct tgtcctaaaa tcaacacttt
    42121 ggtttcttcc aaaggagtga cttgtaaggc tttaaagaca ttttctcgtg gaggatagac
    42181 aagaccggaa gcataggctt catctaaaaa gtgattgatg cgaccgtagt aatgctccgg
    42241 caaaaatgat ttgattttct catgccaaat tgaatgagcc ataagattcc tttctaatca
    42301 cgtcagtttc taagttaaat acgatgtcct tttctatcta gtttaccaca ttttaaatag
    42361 aaagaccctg aaaatatgat atttccaggg ttaatattcg ttttttacag gatattaaac
    42421 aagtgctagc ttattttcta agaattcttg gtaacgttct aacaaaaaag caataatcca
    42481 atagataaag gctactaaaa tgtacatgga catgtagtcc cactccctac caccgataat
    42541 tttggcattt tgaaaaatat ctggtacggt aatcatggca gctagcgacg aacttttaac
    42601 catatcaata atgacattgc ctagaggagg aactgcaatt cgaaaggctt gaggaagaat
    42661 aattttctta accataagag catatggcaa ccctaaagcc ttagctgctt cccactgtcc
    42721 cttgtcaata gcttctatag aagagcgaaa aacttcagaa atataggcag cgctaaccat
    42781 agaaaaacca agataagcgc aaagtaaagc tggcaattct aaaccataat aaggcaaccc
    42841 gaaataaaga acaaagagca ccacgatcat cggcacacct cgcataatag agatgtaggc
    42901 cctaacaaga tagtgtagca gtggctgatg tgaccttcct agtagggcaa ggagcaaacc
    42961 aaagaccagc cccgttaaaa agctaagcaa cgatatgcca agggtataag gtaatcccga
    43021 taatacaaat cctaaactat ctttcattaa tgggatatta atcatgtggt taggactcct
    43081 ttaacatcaa gtattagtta acgtctttcg tatcaatgat gggaattttt ttgtctttgc
    43141 caaaaggttt tgtcaaatct tgaccagcat agtaggtttc agatattttc ttcaagctac
    43201 catcttttat catgtcttta ataacagcat ctattttttt cttgaggcta tcgtcttttt
    43261 tgttcataac aattccttgt tcggttgggt tgtactgagc atcgctcatt ttgacattta
    43321 gatttggatt ttgagataac atgtaatcca caaataattt ttgagctggg taatcatttg
    43381 gaatgaaatc tgttcgaccg ttagccacat cgtttagata aacatctcct gtaacattat
    43441 cgtaggtcac cagttcagct ccctgttttt gcgctacttt catgtactct gttccagaag
    43501 cgccagctgc tttttttcct ttccagtcgc taagatcttt tttagccatg tttgagctgc
    43561 catcagcacg gacaatcatg cctcctaccc cgtatttata aggcgtagag atattgtatt
    43621 ttttctgacg ttttggggta atgtcaaagt tgttcaaaga aatatccact cgaccactat
    43681 caactgaggt aaaggcttgg tcaaatcctg tttcaacaaa cttgacttta atgttaagtc
    43741 gtttaccgat ttccttgacc atatctactt cgtaacctac taactcatta ttatcgttgt
    43801 aaaaagaagt tggctgatag gttcctgggg tagctacttt taacacccct ttttctttga
    43861 tagtctccca acttgatttt tgagatttat gtggcgcaca tgctgccaat aaaaggctgg
    43921 caagtccgat agacagtaaa gtaactgatt tcttcatagt aactccttta ttaagataag
    43981 aatagtataa cattttgatt aagttgtgtt atcagtgagc gctcaatctc tttaaaaagt
    44041 taatctataa tagttaaact gttttataag attattattg aggaatcttc aataatcctg
    44101 ctgcctcaaa ctgctctgca attcctaata ataggtcctc tctacctttg gctgcaatta
    44161 gttgaatgcc cattgggagc ccttccttag tttcgtaagt aggtaaacta atagctggct
    44221 ggccagttag attaggcaga gctgtgtaag gattaatagc cagactctta ccaaacatgg
    44281 tttcgaccaa attgaattgc tcttcactgc tgaaaatttc agcttgagcc aagttagcca
    44341 tgagtttcga atctgggaca agttctccgt gcttaggagc tggtgtgttg gttgtgaacg
    44401 tcaacaacag gtcataagtc tcatgaaaac tagccatggt tgcactgtac gtatcccatt
    44461 tttgaaggac ctgtgagtaa cgccaagctg ggatgtcttt acctgattgg taaatagccc
    44521 aggtcatggt ttccatatcg tctttggtca tcggacgtcc aaaagtgtct tcaatatcag
    44581 caaacatagc agccgtttcc acactattca taatataata atgacgaatc acttctgtca
    44641 tatttacagg aaactcttcc aattcaacta gctgatgccc ttgttctctt aaccaagtta
    44701 ctgcttgtct taaagctttt tcagtatcta aagatactgg gctaccatca cttaaccgtt
    44761 gataaaaagc aattgtcaag ggacgttgta gcgattgata aatactattt tttgttaagg
    44821 tggctagggg gaaggggctt tccatttgtt ccatctgtaa atagtagagt agatttctag
    44881 tatctctaac ggatttggtc aaggcaaaat gaactgaggc tccttgccag cttcgataag
    44941 aaccaggtcc gacaggcatg cgcccgcggc taggtttgag cccaattaag ccattaaacg
    45001 aggctggaat tcgaatagaa ccaccaccat cactagcagt tgctagtgca ctaattcctg
    45061 atgacactag agcagcagct cccccagatg agccaccagc atttcgtgta ttatcatgag
    45121 gtaatgaaac cgggccatgg agctgactat cactaatatt tttaaagcca aattcaggtg
    45181 tgttagaacg ccctaaaata ataaatccca agtcttctaa ccttttcacg aataaatctg
    45241 ttttagtcgc atgatgattc ttgaatagct tagaacccga tgttgataag tctcccttca
    45301 attcttgtcc caaatctttc aaaaaaatgg gaactcctgc aaaaagctta cctgaaaaat
    45361 ccctttgttt ggcttcttct agggctgctt caaagcgttc tgaggtaata gcatttaagg
    45421 tagggttcaa ctttttagcc ttataaatag cctgggttac taattctagt ggtgtggtct
    45481 gaccggtctg aacagcaata gccatagccg tcgcatcttg ataagtcata gctactcttc
    45541 ctttctaaga tatgaatcct aaaacattct tttttattaa aacacaaata aaagaaaacc
    45601 accattaaaa ggttaaaaat tagaatgtga tgtgtaagga atgtagacaa aacaatcaaa
    45661 acataacatt ttgaattggg gttaaagaga ttttttcatt aaaaataaac ctggctgata
    45721 gttccttatt gttgccagtt tacctggtct tctttaaatt ttttaagtaa atgtaaacca
    45781 tcattagtaa tatagccttg caactttgca actgcaatta gctcagtata attagaaagc
    45841 gtaatcaact tgataccagc ttctttaaaa ttctgactag cttttggtaa ctcataagtg
    45901 aaaatcgcaa caactcccag aacgtcagct ccctcacgac tggctgctgc ggctgcatct
    45961 agtaccgaac cacctgttga aatcaaatct tcaatgataa ccattttttg gcctttaagc
    46021 acacgcccct caatctggtt gcctgctcca tgatcttttg gcttactgcg aatataagca
    46081 aaaggaagag tcattttatc tgctataatg gcaccgtgag gaatacctgc tgtagctgta
    46141 ccagcgatga cctcaacttc tgggaaatga gccctgatag tttccacaaa cccattctca
    46201 attaaatcac gagtcttagg ataagaaagg gtgacacgat tatcagtgta aataggtgat
    46261 ttaatgccag atgcccacgt aaaaggatct tcaggcttta aataaacagc tttaatatca
    46321 agtaattgag ttgcaatttg gcttcctagt gtcatttctt attctccttt ttcttcttta
    46381 acagacaagt gttggtttcc taatcttatc tgttagcctg cccactcagc ttttatggct
    46441 tggtaagcag ccactgggtc ttttgcctgg gtaattggtc gccctaccac aatataatca
    46501 cttccaatgc gtcttgcttg gtttggagtc atcactcgtt tttgatcgcc aatattactg
    46561 cctttaggac gaatgccagg agttaagcaa gtaaatcccg taggcgtcac cgccttgata
    46621 gcttctactt cttgtgccga acaaaccacc ccatctagct gagctttagc tgcccccttg
    46681 ctgtaatgca aaacagactc taaaagactc gtttgaatat tttgatcacc acgcatttgg
    46741 tcttcactgg ttgaagttag ctgcgttacc gcaattaagg tagggccttg gcctagtcct
    46801 tctcgagctg cttttagcat ctctacacca ccagctgcat gaacagttgc catatcaata
    46861 tctaattctt taagaactgc catagcagca cgaacagtat ttggaatatc atgtaatttt
    46921 aaatccaaaa atacattatg ccctaacgat ttgatagacc gaacaatatc tggtccttgc
    46981 gcataatata attccatacc aattttcacg tatagttttt cttctgctgg aaataagtct
    47041 aaaaatgctt tagtctcctc aaatgatgaa aagtctaaag caataattgg gcgttcttct
    47101 ttcataactt atcctttcta taatgtgtca atatctcatt aagcaccaaa aagcctcgct
    47161 accaaagaag cgaggctaca gcaaactaaa agggaattcc ctttttttat cttgtatacc
    47221 ttgtttgcct ctctggactc acttaaaggt gtctaaattt aaactattat agctttttta
    47281 aaacaagatg tcaaaacttt ttgttatttg aacacatttt tcctactata tctttttttg
    47341 tacacttacg tcaactcatt ttatcatttt tacccaatca tttattcaaa ctcacctgtg
    47401 ggtaccttag ggctttggta atattttata aaagcttctt cttcaagatt aaccctctgg
    47461 atatgttcat agagcacatc cggaatagga gcttccttaa gtgacaaagc actcactttt
    47521 aaagatgaaa aaacatcact taagacccca aaaactcgaa tgcgtttatc tccaataata
    47581 atttgaaaga cttctttgga ctggcataaa tctatcagat gatccactcc tttaatccaa
    47641 ttatcgacaa tatccaaagt tttgttcatc aacttaaaag ctgattcttc ataccaaaag
    47701 gtagactcaa tcaagcgttg ctgcatatcc aaggtatcaa tggccgcaac aatatctccc
    47761 aactcagact gtactaaatg agctggaaat tcccctccaa gtatagaagg agttcgttct
    47821 acaagcttca gttcatctcg aaaaagagtc aaatagtgga tactgaccaa taacttttgg
    47881 attaagattt gatgactatt ttgttcagta acattcacac gaccaccacc ctttgattag
    47941 aaaaatacct atttgttaat actattaata taacacaaga aaaataaaag ggcaatgaaa
    48001 ttgatagatt caactctcaa aattgaatca tatttatcct aggccatatc gagaaaagac
    48061 tccagcgctt tttcaaagtt ctaaacccat gatgacgttt gtgcttgcgt ctttattttt
    48121 tttgagctag actgtactta tgagaattca acaactgcat tatattatca aaattgttga
    48181 gtgcggctct atgaatgaag ctgctaagca attatttatc acacaaccaa gtctatccaa
    48241 tgcggtcaag gatttggaaa tggagatggg cattaccatt ttcaaccgaa atcctaaagg
    48301 gatcaccttg acaaaagatg gggtggaatt tttatcttat gctcgccaaa ttatagagca
    48361 aacatcactg ttagaagacc gctataaaaa ccacaatact ggccgagagc tctttagcgt
    48421 ttcatctcaa cattatgcct tcgtagtcaa tgcatttgtc tcactcctca agcggaccga
    48481 catgactcgg tacgaactct ttttgcgtga gacaagaact tgggaaatca ttgatgatgt
    48541 caaaaacttt cgatctgaaa ttggtgttct ctttatcaac gactataacc gagatgtctt
    48601 gaccaaatta tttgatgaca atcatttgac tgcctctcca ttatttaagg cacagcctca
    48661 tatttttgtc agcaaatcaa atcctctagc aactaaaagc ctattaaaca tggacgactt
    48721 gagagatttt ccttacctga gctatgacca agggattcac aactcctttt atttttcaga
    48781 agagatgatg tcccagatgc cccatagcaa atcaattgtg gtcagtgacc gtgccaccct
    48841 ctttaactta atgattggtt tagatggcta caccgttgcc agtggtattc taaatagcaa
    48901 tctaaatggt gaccagattg tggctatccc gctagatgtc cctgacgaaa ttgacattgt
    48961 ttttatcaag catgaaaaag ccaacttgtc caaaatgggg gaacgtttta tcgaatacct
    49021 tctcgaagaa gtaacctttg attaatttct ttttcaatcg tcacactaaa aaacacggta
    49081 gtaagacctg tatcggtcaa gttatcgtgt tttttgataa atatcagaca atagattgtc
    49141 tccaaaggcc caactccgtt ttaataatgg cattcatgtc atcaaagtaa aaaggttcac
    49201 gtcggtagat tacaccagaa aaagtcgcca aggtgataat gttttccaaa aaagcctgtg
    49261 aagaaaaatg agtggtgcga tgcagttcct cttcatcaaa aggcttaatc aaatgagcca
    49321 gaggattacg gacagccttt tcaaaatgtc gtaacaaaaa aaccaagtcc ttgatttcct
    49381 gaggtaagga ggtcaacgtc aacatgtctg ctaagctttt ggtcgtcaca ttgcgagact
    49441 gcttttgaga caggtaagcc ttaaaaacct cgtgatcaga ttccaacatg gcctgaaaat
    49501 gccaggtatc atattggtca ttctttgtat caaaaatata agccttgaag tttggaattt
    49561 ctgacgtgat tagtctgaga aaaagtcggt aaagaatagg cgacactgac ctgacgaaat
    49621 caataatagc accgtttttt actttaactt ctaaatccaa aatgtaattg gcaatctgct
    49681 catcttcaag cgattggtta agccataatg aacagtggta ctggtcttca atgatccgat
    49741 tctctgctgc atgttccgcc agaaaggcaa ggttcaattc tctgcgctct tttaacatct
    49801 ctagtaaata gagggcagtt ttagggatat ttggcaaggc tttagcaatt ctaaaggcgt
    49861 gattataagc atagacatcc aaaaggatgt ctagtgtttg attattagtc attggcaatt
    49921 aaggtttcca aggcgacctt catcatatca gtgaaagtgg tttggcgctc ttcagctgta
    49981 gtatcttcgg ttggatcatt caagttatct gagatcgtca tgattccaag ggctttgaca
    50041 tggtgctgag cagctaaata gtaaagagct gctgcttcca tttcgatggc tttaacaccc
    50101 aatttaccaa gagccatatt acgctcaggc atgttagtgt aaaagacatc agaagaaagc
    50161 acattcccaa cgtgagttgt tactcccatt tctctggcga tatggtaagc cttgtcaagt
    50221 aaaccaaaat cagcaatttg tgggaaatca aattctggga agtcattacg gataatattt
    50281 gagttggttg ctgctgcttg ggcaagcacc aattcacgca catgaacttc tgggtcaatc
    50341 gcacccgcag taccaacacg gatcaatgtt ttgacaccgt aatcaacgat taattcacga
    50401 gcatagatag agatcgaagg catccccata ccagttccca tgacagaaac gcggcgccct
    50461 ttgtaagttc ctgtgtagcc aaacatgtta cgcacttcgt taaaacacac tgcatcttct
    50521 aggaaatttt cagcgataaa tttcgcgcgc aaagggtctc caggtagaag aattttatca
    50581 gcaatatcac cttttttggc agaaatatgg atagacatta ttaagatatt aaaggcgaca
    50641 aagaacaaag taaaaatagg aaactgacga cgcatcccag atgctagaca gtttatcttt
    50701 tttacacagt tctccgcccg tattcaattc aatgaataca gttagcccta cctttccttt
    50761 tattttgtag aaggtcagag ggcggacaga aagcaactga aatttaggga actaacgcca
    50821 tgtgtgagca caaaagttag tttatctagt ttccgagctt tccgcccgta ttcaattgaa
    50881 tacaacccca ttcctttcat aattgatatt gaatagaaga agagagctaa aaatcgagtt
    50941 caaataagaa aggactaact ctctttcctt tattatttaa aaattaatac gaagaaatat
    51001 tcgagtccat gcttaattta aaccgaatac gtctctttcc cttcgttggt tatgttagtt
    51061 agaaaagcta gaatctgagt tcaacattca tggaactcac accccgtgat tattttttga
    51121 tttttttatt gcgaacagat gtgccaaact gacgacataa aataacattc ctgtaataat
    51181 gctccagttg attttgttcg tcctacaact cagctaaaat agctttaaca agacctttga
    51241 aatcttcttt aatatgttca gtcacttcaa caacttcttc gtggttcaat tctgattgga
    51301 aaccagctgc aaagttagtg atggctgaaa tgccaagtac tttaagtcca gaatgagcag
    51361 ccacaataac ttctggcaca gttgacatgc cgacagcatc tgctcctaat actttgaaag
    51421 cacggatttc tgctggtgtc tcataagttg gaccagttaa gcccatataa acaccatctt
    51481 ctagtttgat gttcattttt tcagccactt catgtgcctt attgcgataa accttagtat
    51541 aggcatcaga catatcaggg aagcgtggac caaactcgtc caagttttca ccgattagtg
    51601 ggttatttcc ggtcatattg atatggtctg tgattgccat caaggtacct gggccgtagc
    51661 cgataccacc agctgcattt gtgacaagaa caccttcaca tcccagagct ttcatgacac
    51721 gaacagggaa agtcaccact tcaagcgggt ttccttcgta aaaatggaaa cgaccttgta
    51781 gggcaagtac tttacgacca gctaaatcac cgtagactaa ttttccagca tggccaacaa
    51841 ctgttgattt gccccagttt ggaatatctg cataatcaat aacaacagga ttctctactt
    51901 cttcagcaag ttcaccaagt cctgaaccca gaattaatcc aaattctgga gcttcaattc
    51961 ctttagcaac taaaaagtct tttgtttcgt taattttagt catcaatgac atctttttct
    52021 ccttttacct caatagggtt cacaatctcg ccatcctctt taatataagg actaggcagg
    52081 ggaacaggaa gaatatccag taaggtctca gaaggacggc aaagttttac tcctttgcga
    52141 gtcacaacaa taggacgatt gatcaaaatt ggatccgtca acatggcatt aataacgtct
    52201 ttctcagcta ctgcttgatt tgctaatcca tgagcctcaa attcaggaac attggttcgc
    52261 aagagttctc gcgcagagat tcccatcgat tgtaagagct caattaaagt ctcccgatta
    52321 ggtggcgtct gcaaatactc aataatagta ggctcaatac ctgcatggcg aatcatggca
    52381 agaacattac gtgaagtgcc acagtttgga ttatgataaa tcgtgatttc ttccattatc
    52441 aactaatttt cttattttaa gtgaccaagg aatgactccc caatcatggc tatatcaact
    52501 ccaaaatttt ctgcaacagt cgctgaaata tctgcaaaat gcccttgtgg aataagacca
    52561 tttccagtaa aggatgcaga gtaggcaagc aatggaatgt attcacgagt gtggtcggtg
    52621 cctgcatagg ttgggtcatt accatggtca gcagtgatga gaagcaaatc gtcttctttc
    52681 atatttgcga tgatttcagg taggcgattg tcaaattcat gcaagcagtc gcgataaccc
    52741 tctgggtcac gacgatgacc aaagttggca tcaaaatcaa cgaggttagt aaaggagaat
    52801 cccttagtaa actcaggtaa ttgaagagtc ttaataagcg tgtcaatacc atggctgttc
    52861 gacttattgt gtcccatgtc gtttgtaata cctgaaccat taaagatgtc attaatctta
    52921 ccaacggcat aagttgggac acctgcgtca gccagtttgt tcaaaacggt atcttggaaa
    52981 ggagacactg catagtcatg acggttagcc gtacgtgtaa agtttcctgg ttcgcctaca
    53041 taaggacgcg caataatacg gcctaaaagg gcagggcgtt caagcgtgat ggagcgagca
    53101 tattcacaaa tcttgtaaag ctcttccaca ggaataatgt cttcatgggc tgcaatttga
    53161 agcactgggt ctgctgaggt gtaaacaatc aactcacctg tttccatttg acgcgaacca
    53221 aaatcatcaa taaccgcagt tcctgaatag ggtttattag cttcacgaat aattttgcga
    53281 cctgagaatt cttcaatctt agttaaaatc tcttctggga aaccattcca gaaagtgtca
    53341 aaaggttctg taatgttcaa acccataatt tcccaatggc ctgtcatggt atcttttcca
    53401 agagatactt cttccaattt ggtcacgtaa ccagtaggat tatcttctgt tggaacagtt
    53461 tttagtggta ttggacgaga aatgtttcca agtccaattt ttgccatatt tggaaccgat
    53521 aagcctgctg tttctgaaat atgaccgagg gtgtcagaat ctgtatcagc tacaccagca
    53581 ttaaagaatt tgtcagcatc tggtgctgca ccaataccga cagagtctaa aacaaccaag
    53641 tgaatacgat taaattttga cataagtctc caatctaggg gaggttacgg ttgactcccc
    53701 aatacctata aaatacatta acactgttat atgctaagac tagcttgctt taggcacttc
    53761 taaaacagta acgccgtctt tactagcaac aatcacctta tcaaccatac cattaaagag
    53821 accgtgttcc acaacaccaa ctgttccatc taacaggtga ccaaaagcaa caggatcttt
    53881 aatacaaccc aaatcaagat caataatata gttttgcatg tctgtcacca aacgactatc
    53941 acctttcata cgaaaagacg gtttgtaacc agctttttca aagacacgga acaaacgatc
    54001 agcaccatat tgaacgactt cgactggtaa cttaaaggca cccagatgtt ctaccatttt
    54061 agaagcatca acaacccaga tgtattcttt tgttggtgtt gcaacaattt tttccattag
    54121 taaggctgcg ccgccaccct tgattccatt gaaatcctta tccacttcat cagcaccatc
    54181 tacggtgagg tcaatgctat caatatcatc tatcgatttt aaaggaatgc ctaagacttc
    54241 tgcttgcttg ctagtcacac ttgacgtagt cacaccaacc acttgcagtc cttcttgctt
    54301 tacgcgacgg ccaatttcct caacaaaata gtaagccgtt gacccagttc caagcccaat
    54361 ggtcatgcca tcagtcacat attgcgcagc ggtaacaccg gcaatttttt tgagtgcttc
    54421 catgattccc ccttataata gaattgcgtc ttagcacttt attataccat gaaagcgcca
    54481 acttgttaat gaaaacattt gtttttctct cattgacagc ctaagtgcta tagaatagga
    54541 tgatcttttt aaaacttatg atttattcta aagtcatcat ataagcactt acttttcgtg
    54601 tttatttagc tttttgaaaa tcaagtgttt ttgtttgacc tttactgacc ttttggttat
    54661 aataatacta taagatgagt aaaaataatg tctgaatcat tttattagca aagggtttcc
    54721 tatcatgtga aatcctaaca acgattctgc gactgcttac aaacatctta tagtctattt
    54781 tttattgcga ggtaactgat atgacacatt tttctggtaa agatcctttt gtaaatatgg
    54841 atgatatttt caaccaatta atggcaaaca tgggtggtta tcgtagcgaa aatccccgct
    54901 atctcgttaa cggtcgcgaa ataactcccg aagaattcca acactatcgt caaacaggac
    54961 aactccctgt agcgacaacc aaagcaacaa cttctcaaat gttaacacct aaagctgata
    55021 gtgtattgac tcaactcgga actaacttaa cacaagaagc tcgtcaaggg catcttgacc
    55081 ccgtcattgg tcgtaacaaa gaaattcaag ataccgctga aatcttggca cgacgaacta
    55141 aaaataatcc tgtccttgtc ggagatgctg gtgtcggtaa aactgcagtc attgaaggtt
    55201 tagctcaagc cattgttaat ggagatgtcc cagctgctat taaaaacaag gaaatcgtat
    55261 caattgatat ttctagtctt gaagctggta cacagtatcg tggttccttt gaagagacca
    55321 ttcaaaactt gattcaagaa gtaaaagaag ctgggaatat catcttattt tttgatgaaa
    55381 ttcatcaaat agttggtgct ggtgccacaa gcagtgattc aggctcaaag ggacttgcag
    55441 atattcttaa accagccttg tcacgaggag aattaacact gattggtgct actactcaag
    55501 acgaataccg caatactatt ttaaaaaatg ctgcactcgc tcgtcgcttt aatgaagtta
    55561 aagtgaatgc accatcagct gaagatactt ttcacatttt aatgggtatt cgtaatcttt
    55621 atgaacaaca ccaccatatt acccttcctg ataatgttct aaaagctgct gttgattatt
    55681 ctattcaata catcccacag cgtagcctgc cagataaagc aattgactta ctggatatga
    55741 cagctgctca tttagctgcg caacacccgg tcactgactt aaaaacactc gaaactgaaa
    55801 tagctaaaca aaaagagagg caagaaaaag ctgtcgccaa agaagacttt gaaaaagctt
    55861 tagcagcaaa aacacggatt gaaacattac aaaagcaaat tgagcaacat aaccaaagcc
    55921 aaaatgttac tgctacagtc aatgatatcg cagagtctgt agagcgctta actggtattc
    55981 ccgtctctaa catggggact aatgatttag aaaggctcaa aggaattagc agccgcttaa
    56041 aaagccatgt catcggacaa gatgaggctg ttgcagcagt agcacgtgct attcgacgaa
    56101 atcgagcagg ttttgatgat ggtaaccgtc caataggttc tttccttttt gtgggtccaa
    56161 ctggtgttgg taaaacagag ttagctaaac aattagcact tgatttattt ggttccaaag
    56221 atgctattat tcgacttgat atgtccgaat ataacgatcg cactgctgtg tcaaaattga
    56281 ttgggacaac ggctggttat gttggctacg atgataataa taatacccta actgagcgtg
    56341 ttcgtcgtaa cccttatgct attgtcttac tagatgaaat tgaaaaagct gatcctcaaa
    56401 tcattacact tcttctacaa gtgttagatg atggacgatt gacagatgga caaggaaata
    56461 ctattaactt taaaaatacc gttattattg ctacatcaaa tgccggtttt ggccaacaag
    56521 atacggaaac ttctgggttt aacattatgg atcgcattgc accttatttc agaccagagt
    56581 ttttgaaccg ttttaatagt atcattaaat ttaatcatct gcaaaaagaa aatttagaag
    56641 aaattgttga tttaatgcta gctgaagtaa atcaaaccac cgcaaaaaaa ggtattagtt
    56701 taacgattgc tgatgacgct aaagctcatt tgatagattt agggtacaat cacgctatgg
    56761 gagctcgtcc attacgacgt attatcgaac aggaaattcg tgaccgtata actgattatt
    56821 atcttgacca tccagaagtc aaaaaacttc aggcaatcct agaagaagga caactagtga
    56881 ttaggcaaaa agatcagtaa taaacttctt caattgaata attttcaaaa catgaaaaaa
    56941 tccgtcttgt gactacgtta tacacttgac ggactttttt ttagcgcttt tttaaaaata
    57001 attttttcat cgcctaataa ctcaagtttc tttccaaatc tggtgtaatc aaaataacca
    57061 ttaggtgtga gcaatccttt tgaaaaagaa gtatgtcccg ctgctacttc ttctttgata
    57121 tcaatggtgt gcaaaatacg attaactttt cctttgagaa caacagcaga taaatgtcct
    57181 gttttttgat tatatttgcc tccgatctga gaagctaatt tctcaaaagc ttgatctaaa
    57241 atcatttcta attcatggct agctggcata ctattctcaa taatttggcg ttctttacta
    57301 ctcaagtcac caacaactaa atgattttcc tttaatgtct ttaataattc attttggtga
    57361 taagcagcta aacgtttgac aaaaatgtct cgttcaactt gaacactaaa actcaagtca
    57421 acgttgttat ttttaactgt tagtatcatc tgagatgacg ttaataaact atcatcaata
    57481 agagggctct gatgcaaacc agtaagacgt ttagcaaaat catcgcctaa cttctgatca
    57541 atgcttgttg ttcgccagat accttcaata gagctattac gatatcgaac accaaaaagt
    57601 acagcaagca agataagaaa aataataaat acaaaaaggg cattgcattg atggcataac
    57661 aatgactttc ctttagagcg cccttgggct acttgtgtca tgactcatct ccttaatata
    57721 aaatactagc atgtcatcat taaggcgtca agtaaaatcg gtaaacctaa ctatgatttt
    57781 ttatgttttc gataagaaaa acaccctcta ctgagaatgt tttagcatag ataaatcaac
    57841 acctatcgct agacctgctc taaaatagca tcccaagaat catcgatacc aatacgttct
    57901 accgaagaaa atactataaa agtatcagag ttgtcaaaat tcaaagcttt cttgaccaca
    57961 gactcatgtt tgttccattt gccacgagga atcttatctg ctttggttgc cacaacaata
    58021 acaggaatgt catagtactt caaaaagtca tacatctgaa tatcttcttt tgaaggggca
    58081 tgacgtaaat caaccaaact cacgactgca cgcagattgt ctctgctggt taagtattct
    58141 tcgatcatct ttccccattt ggcccgttca cttttagaaa ccttggcata gccataacca
    58201 gggacatcca caaatctaag cttgtcatca atattgaaaa aatttaatag ttgggttttt
    58261 cctggcttgc ttgaggttct tgccaaattt ttgcgcccca atatggtatt aataaaactt
    58321 gatttaccaa catttgaacg tccagcgagg gcaatttctg gcaaatcatc tttggggtaa
    58381 tgagatttat tggcagcact caataaaatt gaggcgttat gggtatttaa gacttgttct
    58441 tcagccatta tttctcctta agctgtctct aaaacgggtt ttgacttgcc ttcgacagca
    58501 gccttggtga tacgcacttt tgtgacatct tcttggcttg gaatttcaaa cataatgtct
    58561 aacatcgttt cttcaataat agaacgaaga ccacgtgctc ctgtcttacg ctcaatggct
    58621 ttattggcaa tggcttctaa ggcttcctta tcaaatgcca attcgacgcc atcgtaagaa
    58681 agaagggcct gatattgctt gaccagggca tttcttggtt ctgtcaaaat ttgaatcaaa
    58741 tctgatgtgt ttagttgttc aagggctgcc acaactggta agcggccaat aaattctgga
    58801 ataagcccaa acttttgaat atcttctgaa ataatttctt gcatataaga agcattgtca
    58861 tcaattttac ggctgttttg accaaaacca ataacttttt cacctaaacg ttgtttaaca
    58921 atctcctcga tgccatcaaa agccccgcca acaataaaca agatattttt tgtatcaatt
    58981 tggatcattt cttgattagg gtgcttccgc ccaccttgtg ggggaacact tgctaccgta
    59041 ccttcaataa ttttaagaag ggcttgctgt acaccttcac cagatacatc acgtgtgata
    59101 gatacgttct cacctttttt agcaatctta tctatttcat caacataaat aataccacgc
    59161 tctgctcgtt cgacattata gtcagcagcc tgaatcagtt tgagtaaaat attttccaca
    59221 tcttctccaa cataccctgc ttcagtcaaa gaagttgcat ctgcaatagc aaatggtaca
    59281 ttgaggcttt tagccagagt ttgtgctaag aaggtttttc ctgaacctgt tggtccaatc
    59341 atcaaaatgt tggacttttg taaatccaca tcatcgtcat cacgactttc tgtaaacgag
    59401 acacgcttat aatgattgta cacagcaact gataaagcac gtttagcacg atcttgacca
    59461 accacatatt gatttaaaac atcaaggagc tcttttggtt ttggaacttc tgtcaaatcg
    59521 gcaaggactt cttcagccaa ttcttctttt ataatttctt gtgataaagc tacgcattca
    59581 ttgcagatga aaacattatt tcctgcgata atttttttaa cgtcatcctg gcttttacca
    59641 caaaatgaac agtatacttt aatatcgtta gttctacttc ctgccatttc ttataattct
    59701 cttactttct cttatctttt actgaatttt aatattagtc aatgtgttgt attggttttt
    59761 agtgattctt gcttgatagc taaatcttca ccagtgctgt cttcgcaagt cacatctcaa
    59821 aacaatgtac catagaaggc gtgaatacta ttaaccagat tagtaaacgc atttaataag
    59881 aacagacttc ctaagccata acgattcttt ttaaaagctg ggatggcaca aagaacacaa
    59941 atcacgcaca aaaaagccgt aaatactcca aaaatactac gttgcatcgc ttatcatctc
    60001 ctcagactat ttgtgtttta aagcagtaac ggtaaaatca taaggatttt tagcgtctcg
    60061 agcatagaac gtttggcttt cttgcgtgaa atgactaaaa tctagatttg gtcggtaggt
    60121 atctccttta aaacgatgat ggataactgt ttttatgata cgatcgaaat aaccattaaa
    60181 cgcttctaaa accttattcc caccaatgat ataaagtgtt ttatcctgcg catggtacca
    60241 ctccaagact ttttcaatac tagtcattgt caacacctca tctacttgat aatctctatt
    60301 tcttgtcatg acaagggttt gacgttgagg aagtcttttg caattcattc cttcaaaagt
    60361 cacacggccc attaagactg cttgatgtag cgttgtcttt ttaaaatgtt ctaactcttt
    60421 tggtaaatac catggtaact tacccgcaac accaataagc cctgcttcat cctctgccca
    60481 aatagcaata atttcctttg tcattaatta tttccttcat tttaaactct atttatttta
    60541 ccaaatttat gaacaaaatg ctcacaaaaa atctaaataa agtttaaata gcaaggtcaa
    60601 agtgcaactg aggcttcact ggatcataat tttgtaattc aaaatcatct ggtttaatat
    60661 caaagaaatt agtcccatct ggaacattta aaacaagctt aggctgacta gcaactggct
    60721 gtcgctttag taattcttga gcctgatcaa attggttgtc ataaatatgt aaattgttaa
    60781 caaaataaaa gaacttacct attttccatc caaaatgttt agcaatcatc atctgcaaag
    60841 caacgtattg catggcattg atgtgatgag caactaatat gtcattagaa cgttgcgtca
    60901 aactagcatc taaataaaga tcttccccta cacgtctaac atcaaacatg atttgaaagg
    60961 cacatggcaa caaccctttt gtctcctcaa atgcttcata gtcccagaga gaaatgacat
    61021 tacgacgatt ccaaggattt tctgctaatt gctttagtat ttttgagatg atatcatgtt
    61081 ttttgactac agcaccataa cgttgcccaa tagtacgtgt ctggtcaact tcccattcat
    61141 tccaataatg aacattatat ttagcttcta gcacatctaa actgttggac tgatcttgat
    61201 atatccaaaa taattccttt atagcagatt taattggaat gggtcgtaaa gttgtgatgg
    61261 gaaactctcc tttagctaaa tcatattcag caaacgcccc agtaatatac ttagaatgag
    61321 ctgttcgacc atctttatat ttaggacgtg cttgctcact caatgatcct tcgtttataa
    61381 ttttttgaat attagcttta aaaatctggt cagcttttgt catctctaat aacctttctg
    61441 taagcatact tcatactatt ctaactaatt ttaccaaaaa ataccagaat aaataaacat
    61501 ttgaaaaact tcatgtggtc atgaacattt taactattaa aggataaaac aaataacttt
    61561 atcagtctaa ttttgcagta aaatgtgata gaatgggtaa gactataaaa ataactaaaa
    61621 gaaggacaaa tatgacaatt ggaattgata agattggttt tgcaaccagt caatatgtct
    61681 tgaaattgga agatttagcg cttgcacgcc aagtggatcc agcaaaattt agtcaagggc
    61741 tactcattga gtcttttagt gtggcaccaa tcactgaaga cattattact ttagctgctt
    61801 ctgcagcaga tcaaatctta accgacgaag atcgatctaa gattgatatg gttattttgg
    61861 caactgaatc gagtactgat cagtcaaagg catcagctat ctatgtacat cacttagttg
    61921 gtatccagcc ttttgcacgt tcctttgaag taaaacaagc ctgctatagc gcaactgctg
    61981 ctctagacta tgctaaactg catgtggctt ctaagccaga ttctcgtgtc cttgttattg
    62041 ctagtgatat tgctagatac ggtgtaggat ctcctggcga atcaactcaa ggctctggta
    62101 gtattgctct tttggtaact gctaaccctc gtattcttgc tctaaatgaa gataatgtgg
    62161 ctcaaactag ggatattatg gacttttgga ggcctaacta tagtttcaca ccttatgttg
    62221 atggtattta ctctaccaag caatatctca attgcttaga aaccacgtgg caagcttatc
    62281 agaaaagaga aaaccttcag ttatctgatt ttgctgcagt ttgtttccat attccatttc
    62341 ccaagttagc cctaaaaggt ctaaataaca ttatggataa cacagtacct cctgaacaca
    62401 gggaaaaact aatagaagcc tttcaagctt ctattactta tagcaaacaa attggaaata
    62461 tttatactgg ctcactttat ctaggattgt tatctttact tgaaaatagt aaagtattac
    62521 aatctggaga taaaattggt ttttttagct atggttccgg tgccgtaagt gaattttatt
    62581 ccggtcagtt agttgctggg tacgataaaa tgttaatgac taatcgacaa gctttactgg
    62641 atcaacgaac acgtctttcc gtttctaaat acgaagacct tttctacgaa caagtccaat
    62701 tagatgataa tggtaatgcc aattttgaca tttacttaac tggaaaattt gctctaacag
    62761 ccatcaagga gcatcgaagg atctatcata ccaatgacaa aaactaatct taactggagc
    62821 ggtttttcaa agaaaacatt cgaagaacgc ctccaactta tcgaaaaatt taaactactt
    62881 aatgctgaaa acttaaatca actcaaaaca gacgttcttt tgcctatcca aacagctaat
    62941 caaatgactg aaaatgtctt aggacgattg gctttgccct ttagcatagc tcctgatttt
    63001 cttgtcaacg gttcaactta tcagatgcct tttgtcacgg aagaaccttc tgttgttgct
    63061 gcagcatctt tcgcagcaaa actaatcaaa cgctcaggtg gttttaaagc tcaaacccta
    63121 aaccgacaaa tgattggtca aattgttctt tacgatatcg accaaataga taacgctaaa
    63181 gccgccatcc ttcataaaac aaaaaagcta attgcattgg caaataaagc ttatccttcc
    63241 attgttaaaa gaggtggagg cgctagaacc attcatttgg aagaaaaagg agagtttttg
    63301 attttctatc tgactgttga tacccaagaa gctatgggag caaatatggt caatactatg
    63361 atggaagctc ttgttcctga tttaacaaga ctgtctaagg ggcattgtct aatggcgatt
    63421 ttatctaatt acgcaacaga gtcgcttgtt actactagtt gtgagattcc cgtgcgcctt
    63481 ttagatcacg ataaaacaaa atccctacag ttagctcaaa aaatagagct agccagccga
    63541 ctagctcaag tagatcctta ccgggctact actcataata aaggtatttt taatggcatt
    63601 gatgcagtgg taatagccac aggaaatgac tggcgtgcta ttgaagcagg ggcccatgct
    63661 tatgcctcaa gaaatggtag ctatcaagga cttagtcagt ggcattttga ccaagataaa
    63721 caagttctgc ttggccaaat gaccctccct atgcctattg ctagtaaggg gggatctatc
    63781 gggcttaacc ctactgtttc tatcgcacat gatcttctta atcaacctga tgccaaaaca
    63841 ttagcccaat tgattgcatc tgtggggtta gctcaaaact ttgctgcact aaaagctctg
    63901 acctcatctg gcatccaaac tggtcacatg aaactacatg cgaaatcatt agctcttttg
    63961 gcgggggcaa cccaagacga aattgctcct ttagttaatg ctttactagc tgataaacca
    64021 ataaatctag aaaaagcaca tttttactta tcccagctaa gacagtctta gatttaaaca
    64081 actaatataa ggcaaacacc catgtaaaca attggtttac atgggtgttt aatctttgtt
    64141 tctatacata caagtcttag aaatagtctc cataacatac tctatttact catctactct
    64201 attctgagtt tgatgaggta tttataaata ttttatatta tgttctaaaa aagctctatc
    64261 atctttgtag taggtggcac tattgtagag gtgatgaagc ctaattaacc tgctgcaagc
    64321 gtccatatag taagtagtcg actcccttta attctttaat agttttacag tcaagagcac
    64381 acataatgat ttttaattct tctttccagc cgttaacgat agcaatcaca cgctcggttg
    64441 ggtatttttc gaccaattct aaaacggtgc gtgagagtcc cactgcacgt gctcctagga
    64501 cgaaacattt aatcatgtcc aaggggtgtc tgacaccacc cgaagctaag atttccactt
    64561 ggtccatcag tccttgtgca tttagcaagc actgaacggt ggtttgtccc caatcgttta
    64621 agtaagagcg gtctcctcct ctttgatttt caatataggc aaatgaggtc cctcctcttc
    64681 ctgaaatatc aaaggtttga atgcctaggt catgtgctag cttgatacta ttgacatcca
    64741 tgccaaaacc aacttctttg agaatgacag gaactggtat ttgactagcg tattcagcga
    64801 ggtgtttttt ccaggtatga aagacgcgct caccctctgg catcagcaac tcttgcatca
    64861 cattcacatg aacctgtaaa aagaggggct gcatttcttg aaccgtttgt tgtcctagcg
    64921 ccacaggttt atctagacca atattcgtgg ctagtttcaa gttatctgcc acctcatgta
    64981 aacgatagga atcgtcgtta ggatttttta aagcagcgct ataagaccct gtcaccatga
    65041 caatccctgt tgctgctgct actttggcca atttttcatt gacagctttg cctttttgac
    65101 ttcctcctgt catggcattg atgtaaaagg gaaagtcgaa gtcttgcccg gcaaaatgag
    65161 tactgagatc aatatcagac aaatcatagc taggtaagga atgatgtatg agttctatgt
    65221 catcaaaagc attataaggc gattggtact tgagggcata tttgatgtga tcatctttac
    65281 ggttggtcat tctcttccca cctttcttga taaagtaatg cgatatcagc ttcttgccat
    65341 ttggaaataa gggtatctct ggcattctga ttaaaggcaa gcgcgatgcc acaatctcca
    65401 ccaccagaac ctgaagattt agcaacagca tcttgcttct gacaagcagc taccaaggtt
    65461 actagcttag gatggtagat agctgaatga agctctttta ggagatgact tgctcctgct
    65521 aaacttttct tgagttcttc cttatgacct tcttgcaaag ctaccatagc tgattgcgtt
    65581 agctggtaac ttgttcttaa gaaagctggg gtaatgctcg ctgtcacttg ttgaatcatc
    65641 tgccttgaaa tagcaggtat tttagtccag ccaaccaaaa agtcacaagg cagggctggt
    65701 tgaatgactt ggatatggta accccaatct ttgacgagaa gtttttttaa gggcatggtc
    65761 tgcagccagt tactgacctg ttctcggtca aaggaggtat aggcgactaa agtctggtaa
    65821 gcgatacagg caatatcccc catagaccca ttatctcctt gcttaagcaa ggtataagcc
    65881 gctagtttaa agagtaactc tggggttaaa gtgatctgat aataggccga taaggccttt
    65941 aaggttaaga gggtgacact accacttgaa ccaatgccaa attttttgcc atcgcgctcc
    66001 atttttcccg ttataattag ggaaaatggc tccagttggt caattgactg tcctagataa
    66061 tcagcaaagg ttttaaccgt tgcttgaatc agtgcataag aggcatcggg tgtcatgccc
    66121 gctttatggg aaaacatatc tgaagctaat tgaatatggg ctgctgggct aatttctgct
    66181 gtcatcatca gtggaataaa gtgaattaag gctttttgtc ctggtgttaa gatagcatat
    66241 tcgcctgtga ggtatagttt cccacctgtt tgcacacaat aattagacat ctggtaaatc
    66301 ctttgttttt gaaacgataa tacggtagtt cttgccaagt cgttctgcta gttgagccaa
    66361 gtctttttct aaacatagga ccttcacatt tggacctgcg tccatggtaa aataacaagc
    66421 aaaaccttct tgacgcagtt ctttcacagc ctccatagcc tggtaagact cttttgttag
    66481 gtaagaaaag ggaggattgg ctgtctttgt cgtggcatgc atggccaagg cattagcttc
    66541 tgttaactga ccaactttct caaaattatt agttttgaga taggtaagca tatgttggta
    66601 atcgattgcc gattgttcta cccattcatc aaatgtagtt gaggtatcgc ggcataactt
    66661 catgccctct cggctagaaa ttggcttttt cgcagcattt aagaccagca taatcatggc
    66721 cattttcaag tcagtctcaa ccttgtaaat agcaccacta tctttgtccc aagcagcaac
    66781 tgggccaaaa aaagaacgag aagatgatcc tgaggcgaac ttagcctttt gagctaaagc
    66841 tttctgatct agctgagtgt caaataattg atcacaggct ttaaccaagg ctgataagcc
    66901 actggaactt gatgataaac ctgcagctgt tggcatatta ttttgagttt ccatctttac
    66961 aaaagcctga ccaggttggc gaaattggtc aatgatagta gaaattttgg tatgttcttc
    67021 gtcattttgc aagacaccgt taatgtaaaa ctgatcactg gttgcagtat ctggtaagaa
    67081 ggaaacgctg gttgtggtga acatgttttc caaagtcaaa gaaatgctag aggtcgaagg
    67141 aatcatctta gcttgattct cttttcccca atatttgata atagcaatgt tagcatagga
    67201 tgttacggtt ataacatttg gatccacgtg tttacggctc cttcttcttt taatctgtga
    67261 ctgatttttt cggccatgtc tttggtatct gctagggcaa tcatacagcc acccaagcca
    67321 ccgcctgtca tcttagcgcc taaagcacct gctcttaggg cagcctccac aagttggtca
    67381 gctttactga tgctaacgcc aatggccttt aaagcagagt gcgcttgtgt catgagctga
    67441 ccgatagcca ccttattttt ttggttaatc gctctttcaa gggcttgtgt caaagctcct
    67501 agtttagcca gatagggcaa attcgtttcc tcaaactgtg ctaccttatt gacggcctca
    67561 cgtgtatgac cgtgaatccc tgtatctgca atgacaagat aaccatttaa ggcgatttcg
    67621 atagtttcaa agccaatgtt tcggataaac ttaatggcat ggtcactaag gcatgtcttg
    67681 gcatctaaac cactaggatt ggtgtgggca ataatttctg ccttgttgac taaaatttcc
    67741 aacaaatcat ccgagagggg ttcttgacaa taagaaaaaa cggctctaat agctgcaata
    67801 gaaacggctg ccgaagaccc cataccacgc ttttgtggca cttgtgaaac aatctcataa
    67861 gcaattggtt cttgtagtcg ctgcaaataa tccaaggcag cataaatggc tgttgataga
    67921 gtatcataaa aatcaaagac caatggctta tcagctggaa aaatatgaca aaccacctca
    67981 atatctgtta aaggcaaagc aatagctggg tagccataca caacagcatg ctcacctatc
    68041 aaaatgatct tactgtgtgc cttaccatat ccaatgtttt cattcatttt ttatcttttc
    68101 attactaatt cttctaaact tttaccttac tattttacac taaatgatta gaaaatgcga
    68161 tgatatatct ttttaaaatc atttcttcac tgaaattaac aataaagact atctactctt
    68221 tttaggatca taacaaaaat tttcttattt caaactacct tctttaatca ctcttctact
    68281 aaattaaacg ttaaaaccag cctcatgggc tggttaagca tcatttatta cctttgccta
    68341 gacattatta ctagctaaag gtactgaaaa atgtttaagt taaatcacca ctgtaaaaac
    68401 aatagcatcg tttttataat ttacagttat cgttccttta aataacttaa ccatactttc
    68461 tgccatagat aaaccaattc catagccttt ttctttgcta ttatgggatt cgtcttcacg
    68521 ataaaagcgt tcaaaaaagc ggctgtaatc aatggatttt ccttccaaat aggtattaga
    68581 aacttctaat ttcgctcgtt ttcgtcttct tccaatagtg gttaaagata ccttgaccaa
    68641 gcctttagga tcacaatatt tattggcatt atcaactaaa attgtcacaa gttcaaataa
    68701 tgacttttcc tccgctttaa tcataatatt cggctgaatc gtcaaatcaa aacgtttacc
    68761 atctttcaaa accagactct taaaatcttc agctgcatct tgagcaatag ctgaaaagtc
    68821 taccatatgt aataccacat ctggctgttc ctccaaacga gccaaagtaa tcatttgatt
    68881 aatcaagcct gtcaatcgct tcacctgatc acttgtactc ttggtccatt ctgattctcc
    68941 agtcattaat tcgaccaatt cattattggc tgagataatg gctagtggtg tttttaactc
    69001 gtggcctgca ttagtaataa agcgacgctg cttctcataa ttttggacaa aaggtttgat
    69061 gaccataccg gaaaaaagac tcactaaaac aacaaaaaag ataaatcctc caaaggccaa
    69121 catcactgaa acagctaata aatctcctac actacgaaaa taaaaggtag tatctaaaat
    69181 tacaaccagt ttttcttcat ttggcagctg agtaatcaag tacgaataaa cactatcttg
    69241 gtgacggtaa ctgccttttt cttccccaga cttagcaaaa agtctagcaa aatattgggc
    69301 ttcttctcta tctaaagctg aaatattagc agtattagaa gaaagtagat gtccattagc
    69361 attaaaaatg acactataat accgaaactg gcttaggcta tcttcagaca gtttcgttcc
    69421 aagtcgttta gagctttccg tagtgccagg taatttacct ttatttgagg aaattaaatg
    69481 taaaatacgg ttaatttctt gttgactttg gtaacagcgt gccgtgttaa taataccaac
    69541 aattgaactt aaaataataa aaatggcaat agaagcaatc atgataaaac gaatacggat
    69601 acgattaaac atgtcacctc ctatttttct aataaacaat agctagtatc tttatcacct
    69661 gcaataatga catttgcttg aatagctaat aatttttgac gaagataaga aatataaacc
    69721 caaacaatag aagtatcata atcttcttgg tctgaccaaa catgttggta aagtgtttta
    69781 gtatccaagt actttccttg atttaacatt aaaaaggcca ttaatttacc ttctttagaa
    69841 gctaatcgta tagcattacc ggcactcaat tcttgctcat taatatttaa tgtgacacca
    69901 gcaaattgta atacctgagg agtaaaactt tctactcgtc tttccatcga ccttaatcta
    69961 gctaaaagtt cttttaatga gaacggttta gtcagataat catcagctcc cgcatctagt
    70021 ccagtaacac ggtcattaat ttctgccata gccgtcagca taataatatg tgagcaattt
    70081 cctgaagcgc gaatctcctt taaagcttca atgccagact taatcggcat catgatatct
    70141 aaaatcataa tatcatatgc attgtcttta gctttatcaa ttgcctcttg accgttaaaa
    70201 accacatcaa cgtcataacc ttgatgagtc atggcagttg tcaaaacatt agacatctgc
    70261 cattcgtctt cagctaacaa aattttcatg gttactcttc ctccgtcgtt tcttgaatca
    70321 attgacgaat ggtttgcatg gtaacatcac aagatgccaa ctcgtctgca caacgcttta
    70381 actctcttgt aatcatttct gcattagcaa cttgatgttt gtacttcatt tgatgttcca
    70441 agctagccca agagtcttga gcaatggttc gcaactgcac ttctataaaa taacatcctt
    70501 ttttttcccc taaaatatct ggaaaatgtg tttctaattc caaaatgaga tgatatgatc
    70561 tatagccatt aggcttggca tttaaaatat aatctttttc agtgttaact gacataccag
    70621 gaatgctttt gagcaagtct accatccgat aaatatcatc aataaaacca caaataatgc
    70681 gaataccgat actatcttta atgattttta atgcagattt gcttgttaga ggaagttgtt
    70741 tgcgttgaca tttttcaatc atcgaagctt ccgatttaat acgggatgta aagtgttcgt
    70801 atagtttaaa ccctgtttct tttttgcttt tgatattctc agcaataatc acatctgtta
    70861 ttgtctgtag taccaacggc aagtacactt caaatccgct atatatagag ctggtagaat
    70921 aatgttctag tctttctttc atacgcttat tataacaaat tttctcaaca aatgcttaaa
    70981 tctctacaat gatcggttat ctttatttct aactatataa ctgtaaacga tctttatcgt
    71041 ttgtttttaa atcgctttca tatatttttt gtgcgttctt ttttaaaaat accattgaat
    71101 tacaaacgaa aacatagtaa acttgatcta taa
//
LOCUS       DATXIG010000005        63095 bp    DNA     linear   BCT 28-DEC-2023
DEFINITION  TPA_asm: Streptococcus pyogenes strain MGAS11113
            SAMN08555799-rid19682583.denovo.005, whole genome shotgun sequence.
ACCESSION   DATXIG010000005 DATXIG010000000
VERSION     DATXIG010000005.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN08555799
            Sequence Read Archive: SRR6740495
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pyogenes
  ORGANISM  Streptococcus pyogenes
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 63095)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 63095)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-OCT-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-OCT-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001918058.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001918058.2
            Genome Coverage       :: 368x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/12/2023 11:23:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 1,806
            CDSs (total)                      :: 1,769
            Genes (coding)                    :: 1,722
            CDSs (with protein)               :: 1,722
            Genes (RNA)                       :: 37
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1, 1 (5S, 23S)
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 47
            CDSs (without protein)            :: 47
            Pseudo Genes (ambiguous residues) :: 0 of 47
            Pseudo Genes (frameshifted)       :: 30 of 47
            Pseudo Genes (incomplete)         :: 20 of 47
            Pseudo Genes (internal stop)      :: 9 of 47
            Pseudo Genes (multiple problems)  :: 11 of 47
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..63095
                     /organism="Streptococcus pyogenes"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN08555799-rid19682583.denovo.005"
                     /strain="MGAS11113"
                     /serotype="Emm28"
                     /isolation_source="normally sterile site"
                     /host="Homo sapiens"
                     /db_xref="taxon:1314"
                     /country="Finland"
                     /collection_date="2002"
                     /collected_by="National Public Health Laboratory"
     gene            48..1244
                     /locus_tag="VC122_000310"
     CDS             48..1244
                     /locus_tag="VC122_000310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014407823.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HEP5640265.1"
                     /translation="MEEKLFNKHFVAITVINFIVYMVYYLFTVIIAFVATRELGAQTS
                     QAGLATGIYILGTLLARLIFGKQLEVFGRRLVLRGGAIFYLLTTLAYFYMPTISMMYL
                     VRFLNGFGYGVVSTATNTIVTAYIPARKRGEGINFYGLSTSLAAAIGPFVGTFMLDNL
                     HIDFRMVIVLCSVLIGCVVVGAFAFPVKNMSLNAEQLAKTKSWTVDSFIEKKALFITA
                     IAFLMGIAYASVLGFQKLYTSEIHLTTVGAYFFVVYALIITITRPAMGRLMDAKGDKW
                     VLYPSYLFLAMGLFLLGSVSSGGSYLLSGALIGFGYGTFMSCGQAASIQGVDEHRFNT
                     AMSTYMIGLDLGLGAGPYLLGLIKDLALGSGVASFRHLFWLAAVIPLICTLLYLLKTK
                     TRQVLS"
     gene            1746..2594
                     /locus_tag="VC122_000311"
     CDS             1746..2594
                     /locus_tag="VC122_000311"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009880787.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aquaporin family protein"
                     /protein_id="HEP5640266.1"
                     /translation="MEMTWTVKYITEFIATAFLIILGNGAVANVDLKGTKGHNSGWLV
                     IAFGYGLGVMMPALMFGNVSGNHINPAFTVGLAVSGLFPWAHVLQYVVAQLLGAIFGQ
                     LVVVMVYKPYFMKTENPNHVLGSFSTISSLDNGQKDSHKVSYINGFLNEFVGSFVLFF
                     GALALTKNYFGVELVGKLIEAGYDQTTAATQISPYVTGSLAVAHIGIGFLVMVLVTSL
                     GGPTGPALNPARDFGPRLLHHFLPKSVLGQAKGDSKWWYAWVPVVAPILAAIVAVAAF
                     KYLYIR"
     gene            complement(2930..3826)
                     /locus_tag="VC122_000312"
     CDS             complement(2930..3826)
                     /locus_tag="VC122_000312"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011106604.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfite exporter TauE/SafE family protein"
                     /protein_id="HEP5640267.1"
                     /translation="MNEMLILRLIQALLVSAMLFIFFMLVKHLKKNKINPFKRFWTGF
                     WIGLLTDALDTLGIGSFATTTTCFKLTKLVTDDRQLPGTMTVAHVLPVLIQSLCFIFV
                     VKVEVLTLLAMAAAAFIGAYLGTHITKNWHAPTVQRILGSLLIIAAIIMIIRIIYHPG
                     EHLSDTIHGLHGIWLFVGIGFNFIVGVLMTMGLGNYAPELIFFSLMGLSPTVAMPVMM
                     LDAAMIMTASSSQFIKANRVSWDGFAGIVSGGIIGVLLAVFFLTNLDINSLKLLVIAI
                     VFFTGGMLIRSSFTSHKQQTNR"
     gene            4124..4876
                     /locus_tag="VC122_000313"
     CDS             4124..4876
                     /locus_tag="VC122_000313"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011528952.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CppA family protein"
                     /protein_id="HEP5640268.1"
                     /translation="MTLMENITFKTPVLRVNDRDLNIAFYQNNLGLRLVSEENAIAIF
                     SSWGEGQECFVIEESPSVRTRAVEGPKKVNTIVIKTNQPKEIEQLLAHGAHYDALFKG
                     QNGYAFETISPEGDRFLLHAEQDIKHLQGTDLPSLEKDATFKGLTQFKFDIIVLNVIS
                     EERSKAFYRDLFSDQLPITMDFIQEEGPDLAIDPHIAWDLEILEFQVSKDYDMKVLKA
                     TLEEDGHKVYIDKKHKVLVLSDPSQIEVWFTK"
     gene            4861..5811
                     /locus_tag="VC122_000314"
     CDS             4861..5811
                     /locus_tag="VC122_000314"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011285135.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-lactamase family protein"
                     /protein_id="HEP5640269.1"
                     /translation="MVYEMTLAVIKCIENHLHKKVYKGASLALFQSGRWQEYHIGTID
                     GRRPVDANLVYDLASVSKVVGVATICNILLNNGTLALDDPLKAYYPSIVDATVTIRQL
                     LTHTSGLDPYIPNRDVLNAQQLRKALNHLTQKGNKNFYYTDVNFLLLGFMLEELFSES
                     LDQIFDKTIFTPFGMYHTSFGPRPEAVPTLKGVSDGEVHDPKAKILKKHSGSAGLFST
                     LADLESFSNHYLNDPFSDCLWRNYSQQTIERSLGWNLDGDWISHTGYTGPFLMLNKKE
                     QTAAIFLTNRTYDEDDKSKWLKERQLLYNALKHDLTTPVS"
     gene            complement(5991..8318)
                     /gene="pflB"
                     /locus_tag="VC122_000315"
     CDS             complement(5991..8318)
                     /gene="pflB"
                     /locus_tag="VC122_000315"
                     /EC_number="2.3.1.54"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014407821.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate C-acetyltransferase"
                     /protein_id="HEP5640270.1"
                     /translation="MATVKTNTDVFEKAWEGFKGTDWKEKASVSRFVQANYTPYDGDE
                     SFLAGATERSLHIKKVIEETKAHYEATRFPYDTRPTSIADIPAGFIDKENELIYGIQN
                     DELFKLNFMPKGGIRMAETTLKENGYEPDPAVHEIFTKYVTTVNDGIFRAYTSNIRRA
                     RHAHTVTGLPDAYSRGRIIGVYARLALYGADYLMQEKVNDWNAITEIDEESIRLREEV
                     NLQYQALGEVVKLGDLYGVDVRRPAQNVKEAIQWVNIAFMAVCRVINGAATSLGRVPI
                     VLDIFAERDLARGTFTESEIQEFVDDFVLKLRTVKFGRTKAYDALYSGDPTFITTSMA
                     GMGNDGRHRVTKMDYRFLNTLDNIGNSPEPNLTVLWTDQLPETFRRYCMKMSHKHSSI
                     QYEGVTTMAKEGYGEMSCISCCVSPLDPENEEQRHNIQYFGARVNVLKALLTGLNGGY
                     DDVHRDYKVFNVVEPITSEVLEYDEVMANFEKSLDWLTDTYVDALNIIHYMTDKYNYE
                     AVQMAFLPTHQRANMGFGICGFANTVDTLSAIKYATVKTIRDENGYIYDYEVTGDFPR
                     YGEDDDRVDDIAKWLMEAYHTRLASHKLYKNAEASVSLLTITSNVAYSKQTGNSPVHR
                     GVFLNEDGTVNTSQVEFFSPGANPSNKAKGGWLQNLNSLAKLEFSHANDGISLTTQVS
                     PRALGKTFDEQVDNLVTVLDGYFENGGQHVNLNVMDLNDVYDKIMNGEDVIVRISGYC
                     VNTKYLTPEQKTELTQRVFHEVLSMDDAAEAISSK"
     gene            8527..9621
                     /gene="dinB"
                     /locus_tag="VC122_000316"
     CDS             8527..9621
                     /gene="dinB"
                     /locus_tag="VC122_000316"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011285134.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase IV"
                     /protein_id="HEP5640271.1"
                     /translation="MLIFPLINDTSRKIIHIDMDAFFAAVEERDNPALKGKPVVIGKD
                     PRETGGRGVVSTCNYEARKYGIHSAMSSKEAYERCPKAIFISGNYEKYRTVGDQIRRI
                     FKRYTDVVEPMSIDEAYLDVTNNKLGIKSAVKIAKLIQHDIWKEVGLTCSAGVSYNKF
                     LAKLASDFEKPHGLTLVLKEDALCFLAKLPIEKFHGVGKKSVEKLHDMGIYTGQDLLA
                     VPEMTLIDHFGRFGFDLYRKARGISNSPVKSDRIRKSIGSERTYAKLLYQETDIKAEI
                     SKNAKRVAALLQDHKKLGKTIVLKVRYADFTTLTKRVTLPELTRNAAQIEQVAGDIFD
                     SLSENPAGIRLLGITMTNLEDKVADISLDL"
     gene            complement(9714..10196)
                     /locus_tag="VC122_000317"
     CDS             complement(9714..10196)
                     /locus_tag="VC122_000317"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012560937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEP5640272.1"
                     /translation="MISYEKVRQALKTSTIAIIILNGLGVVLSLMGFAGIFYLQSQLK
                     NEAFRAQLTTEQLAQLQSSMTPFMIFLSILNVLAIIAIIVFCAQNLSKLKQGLTVSYI
                     PYILGLILSVIGLVNQFTTTMSMVGTILILIQAALYGFAFYKAKTLNEKGDDTDQAML
                     "
     gene            complement(10287..12650)
                     /locus_tag="VC122_000318"
     CDS             complement(10287..12650)
                     /locus_tag="VC122_000318"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002983069.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent RecD-like DNA helicase"
                     /protein_id="HEP5640273.1"
                     /translation="MEYVFTGTVDRIIFENQANFFKILLLAIEDTDSDIDDFEIIITG
                     TMADIIEGDDYTFWGELTQHPKYGQQLKLSRYQKIKPSSSGLVNYFSSDHFKGIGKKT
                     AEKIIALYGHNTIDHILEDPSKLETISGLSKANRQAFVAKLKLNYGTEQLIAGLVELG
                     LSNRFAFQAFEKYKEEALDLVKENPYQLVEDLQGFGFKMADALAENLGIESGSPKRFR
                     AALLHCLLEESINRGDTYVQARQLLDFAITLLEDARQVECDPAAVAEQLSELIIEGKI
                     KNSDTKLFDASLYFAEEGIANNISRLLDTPLSQSFSHDTIQTTIQAVQKDFAITYDQV
                     QQEAITKALTSKVFLLTGGPGTGKTTVIRGILQAYANLHQIDLDKKDLPILLAAPTGR
                     AARRMNELTGLPSATIHRHLGLNGDNDYQAMEDYLDCDLLIVDEFSMVDTWLANQLLG
                     AINSTTQVIIVGDSDQLPSVGPGQVLSDLLKVNSLPQIALQKIFRQSQESTIVNLADQ
                     MRRGILAADFRDKKADRSYFEAQAAFIPDMIQKIVLSAIKSGIPAEEIQILAPMYKGQ
                     AGINHLNQLMQELLNPLQGQTEFLFNDTHFRKGDKVLHLVNDAQLNVFNGDIGYITDL
                     IPAKYTESKQDELILDFDGSEVTYPRNEWLKLTLAYAMSIHKSQGSEFQVVILPITRQ
                     SGRLLQRNVIYTAITRSKSKLILLGEYTAFEYAIKHEGDKRQTYLIERFQEQSDLASS
                     QSTNSNPTENSQSDNDDFRLTPENYSTIDSMIGLTESDIALFFQKKS"
     gene            complement(12708..13301)
                     /gene="lepB"
                     /locus_tag="VC122_000319"
     CDS             complement(12708..13301)
                     /gene="lepB"
                     /locus_tag="VC122_000319"
                     /EC_number="3.4.21.89"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002983074.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="signal peptidase I"
                     /protein_id="HEP5640274.1"
                     /translation="MKQFIKEWGPFTLFLILFGLSRLFLWQAVKVDGHSMDPTLAHGE
                     RLIVFNQARIDRFDIVVAQEEENGQKKEIVKRVIGLPGDTISYNDDTLYINGKKTVEP
                     YLAEYLKQFKNDKLQKTYAYNTLFQQLAETSDAFTTNSEGQTRFEMSVPKGEYLLLGD
                     DRIVSRDSREVGSFKKENLIGEVKARFWPLNKMTVFN"
     gene            complement(13312..14214)
                     /locus_tag="VC122_000320"
     CDS             complement(13312..14214)
                     /locus_tag="VC122_000320"
                     /EC_number="3.1.26.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002983077.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease HIII"
                     /protein_id="HEP5640275.1"
                     /translation="MNTLVLKIDAILSKHLKKQLAPYTISSQNIYVAFAAKKNGVTVL
                     LYKSGKLVLQGNGANALAQELNLPVAKTVFEASNNSQDIPIIGSDEVGNGSYFGGIAV
                     VASFVDPKDHPFLKKLGVDDSKKLSDKTIQQIAPLLEKQIPHQSLLLSPKKYNELVGK
                     SKPYNTISIKVALHNQAIFLLLQKGIQPKQIVIDAFTSQSNYEKHLKKEKNHFPNPLT
                     FQEKAESHYLAVAVSSIIARNLFLDNLDQLGQDLGYQLPSGAGSASDKVASQLLAAYG
                     MSSLEYSAKLHFANTHKAQALLTK"
     gene            14371..14679
                     /locus_tag="VC122_000321"
     CDS             14371..14679
                     /locus_tag="VC122_000321"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003045196.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEP5640276.1"
                     /translation="MKSINRYKFTFGEKTLTLTTDKDNLFMEEVERVAKEKYQALKNH
                     LPEADDETIAILMAINTLSTQLSREIAIEKMEAEILDLRQKTLVGLQEKANQIDVNEV
                     "
     gene            14682..15227
                     /locus_tag="VC122_000322"
     CDS             14682..15227
                     /locus_tag="VC122_000322"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012560935.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CvpA family protein"
                     /protein_id="HEP5640277.1"
                     /translation="MLSLLIVLVLTWNFYIGYSRGIILQSFYVLGALLSLLVANRFYI
                     GLAHKLTLWIPYSNPVEGTSVFFFKSVDIFVLDKVYYAGLAFFIIFLLGYALSRFLGI
                     FVHFLLLDYFDNQWTKCLSGGLALLVSLLFLNMLLSIFATVPMPFLQHYLHSSFLARL
                     VIEHLPPLTIIIQKLWIQAII"
     gene            15376..17715
                     /locus_tag="VC122_000323"
     CDS             15376..17715
                     /locus_tag="VC122_000323"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017650215.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease MutS2"
                     /protein_id="HEP5640278.1"
                     /translation="MNNKILEQLEFNKVKELLLPYLKTEQSQEELLELEPMTEAPKIE
                     KSFNEISDMEQIFVEHHSFGIVSLSSISESLKRLELSADLNIQELLAIKKVLQSSSDM
                     IHFYSDLDNVSFQSLDRLFENLEQFPNLQGSFQAINDGGFLEHFASPELERIRRQLTN
                     SERRVRQILQDMLKEKAELLSENLIASRSGRSVLPVKNTYRNRISGVVHDISSSGSTV
                     YIEPRAVVTLNEEITQLRADERHEEGRILHAFSDLLRPHVATIRNNAWILGHLDFVRA
                     KYLFMSDNKATIPKISDDSTLALINVRHPLLSNPVANDLHFDHDLTAIVITGPNTGGK
                     TIMLKTLGLAQLMGQSGLPVLADKGSKIAVFNNIFADIGDEQSIEQSLSTFSSHMTHI
                     VSILNEADRNSLVLFDELGAGTDPQEGASLAMAILEHLRLSHIKTMATTHYPELKAYG
                     IETNFVENASMEFDAETLSPTYRFMQGVPGRSNAFEIASRLGLAPFIVKQAKQMTDSD
                     SDVNRIIEQLEAQTLETRRRLDHIKEVEQENLKFNRAVKKLYNEFSHERDKELEKIYQ
                     EAQEIVDMALNESDTILKKLNDKSQLKPHEIIDAKAQIKKLAPQVDLSKNKVLNKAKK
                     IKAARAPRIGDDIIVTSYGQRGTLTSQLKDGRWEAQVGIIKMTLTQDEFSLVRVQEEQ
                     KVKNKQINVVKKADSSGPRARLDLRGKRYEEAMQELDHFIDQALLNNMGQVDIIHGIG
                     TGVIREGVTKYLRRHKHVKHFAYASQNAGGSGATIVTLG"
     gene            17719..18219
                     /locus_tag="VC122_000324"
     CDS             17719..18219
                     /locus_tag="VC122_000324"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017767232.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatase PAP2 family protein"
                     /protein_id="HEP5640279.1"
                     /translation="MESYEHFYAKLSQPFRKKPQLIILLNVLLKIITGVMYILYPSFL
                     IFTLWQGMTFQLWLRLLIIPAVGFIALSYIRKRFDFPRPYEKWNIKPLIDKDTKGRSM
                     PSRHVFSATMISMCLLRYYVYFGIVCLILSVLLAICRVIAGIHYPKDVIVGYLIGLML
                     GLCLLI"
     gene            18300..18614
                     /gene="trxA"
                     /locus_tag="VC122_000325"
     CDS             18300..18614
                     /gene="trxA"
                     /locus_tag="VC122_000325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001162959.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin"
                     /protein_id="HEP5640280.1"
                     /translation="MALEVTDATFVEETKEGLVLIDFWATWCGPCRMQAPILEQLSQE
                     IDEDELKILKMDVDENPETARQFGIMSIPTLMFKKDGEVVKQVAGVHTKDQLKAIIAE
                     LS"
     gene            complement(18666..19254)
                     /locus_tag="VC122_000326"
                     /pseudo
     CDS             complement(18666..19254)
                     /locus_tag="VC122_000326"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000402392.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
     gene            complement(19330..20454)
                     /gene="mutY"
                     /locus_tag="VC122_000327"
     CDS             complement(19330..20454)
                     /gene="mutY"
                     /locus_tag="VC122_000327"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015632237.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="A/G-specific adenine glycosylase"
                     /protein_id="HEP5640281.1"
                     /translation="MWDNETIASFRRTLLGWYDQEKRDLPWRRTTNPYYIWVSEIMLQ
                     QTQVNTVIPYYKRFLEWFPQIKDLADAPEEQLLKAWEGLGYYSRVRNMQKAAQQVMVD
                     FGGIFPHTYDDIASLKGIGPYTAGAIASISFNLPEPAVDGNVMRVMARLFEVNYDIGD
                     PKNRKIFQAIMEILIDPDRPGDFNQALMDLGTDIESAKTPRPDESPIRFFNAAYLNGT
                     YGKYPIKNPKKKPKPMRIQAFVIRNQNGQYLLEKNTKGRLLGGFWSFPIIETSPLSQQ
                     LDLFDDNQSNPIIWQTQNETFEREYQLKPQWTDNHFPNIKHTFSHQKWTIELIEGVVK
                     ATDLPNAPHLKWVAIEDFALYPFATPQKKMLETYLKQKNA"
     gene            20652..20945
                     /locus_tag="VC122_000328"
     CDS             20652..20945
                     /locus_tag="VC122_000328"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002983113.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEP5640282.1"
                     /translation="MFQKLKNLIDRYSWFVFIMMILVLFLNYIYPFDPSLSGLQLEWL
                     IMTQVFPGLGIVCAMVAIIFKKFNLFVLALILYFALWLKLFLAFSFFTYFLGV"
     gene            21118..21408
                     /locus_tag="VC122_000329"
     CDS             21118..21408
                     /locus_tag="VC122_000329"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018030687.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S6"
                     /protein_id="HEP5640283.1"
                     /translation="MAKYEILYIIRPNIEEEAKNALVARFDSILTDNGATVVESKDWE
                     KRRLAYEINDFREGLYHIVNLEATDAAALNEFDRLSKINGDILRHMIVKLDA"
     gene            21430..21921
                     /locus_tag="VC122_000330"
     CDS             21430..21921
                     /locus_tag="VC122_000330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003083754.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="single-stranded DNA-binding protein"
                     /protein_id="HEP5640284.1"
                     /translation="MINNVVLVGRMTKDAELRYTPSQVAVATFTLAVNRTFKSQNGER
                     EADFINCVIWRQPAENLANWAKKGALIGVTGRIQTRNYENQQGQRVYVTEVVADNFQM
                     LESRATREGGSTGSFNGGFNNNTSSSNSYSAPAQQTPNFGRDDSPFGNSNPMDISDDD
                     LPF"
     gene            22086..22325
                     /locus_tag="VC122_000331"
     CDS             22086..22325
                     /locus_tag="VC122_000331"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002983142.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S18"
                     /protein_id="HEP5640285.1"
                     /translation="MAQQRRGGFKRRKKVDFIAANKIEYVDYKDTELLSRFVSERGKI
                     LPRRVTGTSAKNQRKVTTAIKRARVMALMPYVNED"
     gene            complement(22458..23114)
                     /locus_tag="VC122_000332"
     CDS             complement(22458..23114)
                     /locus_tag="VC122_000332"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011285125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1129 domain-containing protein"
                     /protein_id="HEP5640286.1"
                     /translation="MELQELTKKNQEFIHTATNKLIQDGKSDEDIKLILEEAIPAILE
                     NQKKGVTARNLLGTPTAWAASFSQDPSQKAAETDKNTNPWLMWLDTSLLFIGIVALLN
                     GIMTFFNTNATVTGLISLLALGFGGGASMYATYYFIYRHLGKDKSLRPSWFKIIAALS
                     LAMLIWIALYSATAFLPTSLNPQLPPLALLIIGGISLALRYYLQRKYNIQNTMSPVNK
                     "
     gene            complement(23247..24191)
                     /locus_tag="VC122_000333"
     CDS             complement(23247..24191)
                     /locus_tag="VC122_000333"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003085337.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="magnesium transporter CorA family protein"
                     /protein_id="HEP5640287.1"
                     /translation="MKQMFLSSAIEFKEIETFEPGAWIKLVNPSQEESMKIADQFNID
                     ISDLRAPLDVEETSRIAVEDDYTLIIVDVPIYEERNNKSYYITMPLGIIVTENAVITT
                     CLHDMTLFDHFHNRRVKNFYTFMKTRFVFQILYRNAELFLTALRTIDRQSERLEAQLE
                     AATRNEELIDMMELEKSIVYLKASLKFNERIVKKLSSSTSSLKKYIEDEDLLEDTLIE
                     TQQAIEMAGIYENVLNAMTETTASIINNNQNTIMKTLALMTMALDIPTVIFSAYGMNF
                     QNNWLPLNGLEHAFWYITLIAMLLSSFVVIYFIRKKWF"
     gene            24394..27222
                     /gene="uvrA"
                     /locus_tag="VC122_000334"
     CDS             24394..27222
                     /gene="uvrA"
                     /locus_tag="VC122_000334"
                     /EC_number="3.1.25.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002993235.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="excinuclease ABC subunit UvrA"
                     /protein_id="HEP5640288.1"
                     /translation="MQNKIIIHGARAHNLKNIDVEIPRDKLVVVTGLSGSGKSSLAFD
                     TIYAEGQRRYVESLSAYARQFLGNMEKPDVDSIDGLSPAISIDQKTTSKNPRSTVGTV
                     TEINDYLRLLYARVGTPYCINGHGAITASSAEQIVEQVLALPERTRMQILAPIVRRKK
                     GQHKTIFEKIQKDGYVRVRVDGDIFDVTEVPELSKSKMHNIEVVIDRLVNKDGIRSRL
                     FDSVEAALRLGDGYLMIDTMDGNELLFSEHYSCPVCGFTVPELEPRLFSFNAPFGSCP
                     TCDGLGIKLEVDLDLVVPDPSKSLREGALAPWNPISSNYYPTMLEQAMASFGVDMDTP
                     FEALTEEERDLVLYGSGDREFHFHYVNDFGGERNIDIPFEGVVTNVNRRYHETNSDYT
                     RNVMRGYMNELTCATCHGYRLNDQALCVHVGGEEGPHIGQISELSIADHLQLLEELEL
                     TENESTIAKPIVKEIHDRLTFLNNVGLNYLTLSRAAGTLSGGESQRIRLATQIGSNLS
                     GVIYILDEPSIGLHQRDNDRLIESLKKMRDLGNTLIVVEHDEDTMMQADWLIDVGPGA
                     GEFGGEIIASGTPKQVAKNKKSITGQYLSGKKFIPVPLERRSGNGRFIEIKGAAQNNL
                     QSLDVRFPLGKFIAVTGVSGSGKSTLVNSILKKAVAQKLNRNADKPGKYHSISGIEHI
                     ERLIDIDQSPIGRTPRSNPATYTGVFDDIRDLFAQTNEAKIRGYKKGRFSFNVKGGRC
                     EACSGDGIIKIEMHFLPDVYVPCEVCHGRRYNSETLEVHYKEKNIAEVLDMTVDDALV
                     FFSAIPKIARKIQTIKDVGLGYVTLGQPATTLSGGEAQRMKLASELHKRSTGKSLYIL
                     DEPTTGLHTDDIARLLKVLERFVDDGNTVLVIEHNLDVIKSADHIIDLGPEGGVGGGQ
                     IVATGTPEEVAQVKESYTGHYLKVKLQQ"
     gene            27338..28411
                     /locus_tag="VC122_000335"
     CDS             27338..28411
                     /locus_tag="VC122_000335"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012560927.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminopeptidase P family protein"
                     /protein_id="HEP5640289.1"
                     /translation="MSGFLEQRLGHCLRQMAEKGLEALLVTHLTNSYYLTGFSGTAAT
                     VLITAKRRVLITDSRYTLLAKASVEGFDIIESRTPLKVVAELLEADQIDCLGFEDQVS
                     FSFYQAMQAELSGITLLAQSGFVEHLRFIKDTSEIDTIAKACSISDKAFEDALDFIKP
                     GTTTERDLANFLDFRMRQYGASGTSFDIIVASGYRSAMPHGRASDKVIQNKESLTMDF
                     GCYYNHYVSDMTRTIHIGQVTDEEREIYALVLAANKALIDKASAGMTYSDFDGIPRQL
                     ITEAGYGSHFTHGIGHGIGLDIHENPFFGKSEQLLQAGMVVTDEPGIYLDNKYGVRIE
                     DDLVITETGCQVLTLAPKELIVL"
     gene            28445..28912
                     /locus_tag="VC122_000336"
     CDS             28445..28912
                     /locus_tag="VC122_000336"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003059460.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="competence protein ComE"
                     /protein_id="HEP5640290.1"
                     /translation="MTNRLSWQDYFMANAELISKRSTCDRAFVGAVLVKDNRIIATGY
                     NGGVSATDNCNEAGHYMEDGHCIRTVHAEMNALIQCAKEGISTDGTEIYVTHFPCINC
                     TKALLQAGIKKITYKAHYRPHPFAIELMEKKGVAYVQHDVPHIILGGKEGPNG"
     gene            29000..29557
                     /gene="efp"
                     /locus_tag="VC122_000337"
     CDS             29000..29557
                     /gene="efp"
                     /locus_tag="VC122_000337"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000568636.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor P"
                     /protein_id="HEP5640291.1"
                     /translation="MIEASKLKAGMTFESEGKLIRVLEASHHKPGKGNTIMRMKLRDV
                     RTGSTFDTTYRPDEKFEQAIIETVPAQYLYKMDDTAYFMNTDTYDQYEIPVANVEQEL
                     LYILENSDVKIQFYGSEVIGVTVPTTVELTVAETQPSIKGATVTGSGKPATLETGLVV
                     NVPDFIEAGQKLIINTAEGTYVSRA"
     gene            29603..29992
                     /locus_tag="VC122_000338"
     CDS             29603..29992
                     /locus_tag="VC122_000338"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014335250.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Asp23/Gls24 family envelope stress response
                     protein"
                     /protein_id="HEP5640292.1"
                     /translation="MTTEHIGEIVISPRVLEVITGIATTQVEGVHSLHNKKMADSFNK
                     ASLGKGVYLQTEEDGSVTADIYVYLQYGVKVPTVSMNIQKTVKSAVYDMAEVPISAVN
                     IHVEGIVAEKTPKPDLKSLFDEDFLDD"
     gene            29985..30437
                     /gene="nusB"
                     /locus_tag="VC122_000339"
     CDS             29985..30437
                     /gene="nusB"
                     /locus_tag="VC122_000339"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011285698.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription antitermination factor NusB"
                     /protein_id="HEP5640293.1"
                     /translation="MTNSFQNSRRDLRERAFQALFNIEMGAELLAASQFAYGYDKVTG
                     EDAQVLELPIFLLSLVTGVNNHKEELDNLISTHLKKGWSLERLTLTDKTLLRLGLFEI
                     KYFDETPDRVALNEIIEVAKKYSDETSAKFINGLLSQYVSEAPSANKS"
     gene            complement(30578..31543)
                     /locus_tag="VC122_000340"
     CDS             complement(30578..31543)
                     /locus_tag="VC122_000340"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002988504.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HEP5640294.1"
                     /translation="MVAKLTDVAALAGVSPTTVSRVINQKGYLSQKTVNKVNKAMREL
                     GYKPNNLARSLQGKSTQLIGLIFPNISNIFYAELIEHLEIELFKQGYKTIICNSEHNP
                     VKEREYLEMLAANQVDGIISSSHNLGIEDYERVEAPIVAFDRNLAPNIPVISSDNFEG
                     GKLAAQTLQKHGCQNIVMITGNDNSDSPTGLRQLGFNYQLKRSAEIIKLPNNLSPVRR
                     EMEIKSILATRKPDGLFVSDDLTAILIMKVAKQLHITIPEDMKVIGYDGTTFIQQYVP
                     QLATIRQPIDEIAKLSVEILIKKIKKEKTSKDYILPITLLPGASI"
     gene            complement(31548..32987)
                     /locus_tag="VC122_000341"
     CDS             complement(31548..32987)
                     /locus_tag="VC122_000341"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011054964.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 32 protein"
                     /protein_id="HEP5640295.1"
                     /translation="MDLPQAIRYRPYKEWSSKDYQAITEKMAQSPWHSQFHVEPKTGL
                     LNDPNGFSYFNGHYHLFYQNWPYGAAHGLKQWVHMTSTDLVHFTETRSRLLPDHAHDS
                     HGAYSGSAYAIDDKLFLFYTGNVRDANWVRTPLQVGAWMDKQGNISKIPQVLIEQPDD
                     VTEHFRDPQLFSYQGQFYAIIGAQGLDGKGKIKLYKAVDNHVDNWRFIADLDFDDSGT
                     EYMIECPNLVFVDDKPVLIFSPQGLAKADLDYQNIYPNTYKIFESFNPETGQLLGGGA
                     LQNLDFGFEAYATQAFSSPDGRVLAVSWIGLPDIDYPTDRYDYQGALSLVKELRIKDG
                     ILYQTPVSALQNLRGPAELFHNKIDSSNCYELELTIPGQKKLDLLLFADQKGNGLRLK
                     VDTTKGQLSIDRSRAGVQYAQDYGTVRSCQIPQGHVTLNVYVDNSILEIFINQGQKVL
                     TSRVFPTQGQTGIQVVEGQAFGHYYEMRY"
     gene            33229..35112
                     /locus_tag="VC122_000342"
     CDS             33229..35112
                     /locus_tag="VC122_000342"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002995059.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS beta-glucoside transporter subunit IIBCA"
                     /protein_id="HEP5640296.1"
                     /translation="MDNRQIATEVIEALGGRENVRSVAHCATRLRVMVHDEGKIDKEK
                     AEAIDKVKGAFFNSGQYQMIFGTGTVNNIYDEVVALGLPTSSTSEQKAEAGKQGNIFQ
                     RAIRTFGDVFVPIIPAIVATGLFMGVRGLVTQPAIMDLFGVHEYGENFLMYTRILTDT
                     AFVYLPALVAWSAFRVFGGNPIIGIVLGLMLVSNELPNAWVVASGGDVKPLTFFGFVP
                     VVGYQGTVLPAFFVGLVGAKLEKWLHKKVPEALDLLVTPFLTFAIMSTLGLFVIGPVF
                     HSLENLVLAGTQAVLHLPFGIAGLIVGGIQQLIVVTGIHHIFNFLEAQLIANTGKDPF
                     NAYLTAATAAQAGATLAVAVKTKSTKLKGLAFPSTLSALLGITEPAIFGVNLRYPKVF
                     VSGLIGGALGGWVAGLFGIAGTGFGITVLPGTLLYLNGHLLQYLVTMLVGLGVAFGIA
                     YTWGYQDRETLPLPAVEVDQTADQPALAEETLYSPLNGTVVDLAAVSDPVFSSGAMGQ
                     GLAIKPEDNTLYSPVDGKVEIVFETGHAYAITSSQGAEVLLHIGIDTVSMAGDGFESL
                     VAVGQAVKKGDLLGHFDPSKIAEAGLDDTTMMIVTNSADYQSVDILAQGHVLIGDQVA
                     LIK"
     gene            35343..38357
                     /locus_tag="VC122_000343"
     CDS             35343..38357
                     /locus_tag="VC122_000343"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002988512.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="endo-beta-N-acetylglucosaminidase"
                     /protein_id="HEP5640297.1"
                     /translation="MDKHLLVKRTLGCVCAATLMGAALATHHDSLNTVKAEEKTVQVQ
                     KELSSIDSLHYLSENSKKEFKEELSKAGQESQKVKEILAKAQQADKQAQELAKMKIPE
                     KIPMKPLHGPLYGGYFRTWHDKTSDPTEKDKVNSMGELPKEVDLAFIFHDWTKDYSLF
                     WKELATKHVPKLNKQGTRVIRTIPWRFLAGGDNSGIAEDTSKYPNTPEGNKALAKAIV
                     DEYVYKYNLDGLDVDVEHDSIPKVNGEASDENLKRSIDVFEEIGKLIGPKGADKSRLF
                     IMDSTYMADKNPLIERGAPYIDLLLVQVYGARGEQGEFQNDTKLVTDTPEERWQGYSK
                     YIRPEQYMIGFSFYEERAGSGNLWYDINSRKDEDKANGINTDITGTRAERYARWQPKT
                     GGVKGGIFSYAVDRDGVAHQPKQIAEKDKQNVKNNQPQIPEITDNIFHSDYSVSKALK
                     TVMLKDKSYDLIDEKDFPDKALREAVMAQVGTRKGDLERFNGILRLDNPAIQSLEGLN
                     KFKKLAQLDLIGLSRITKLDQSVLPANMKPGKDTLETVLETYKKDNKEEPATIPPVSL
                     KVSGLTGLKELDLSGFDRETLAGLDVATLTSLEKVDISGNKLDLAPGTENRQIFDTML
                     STVSNHVGSNEQTVKFDKQKPTGHYPDTYGKTSLRLPVANGKVDLQSQLLFGTVTNQG
                     TLINSEADYKAYQNQQIAGRSFVDSNYHYNNFKVSYENYTVKVTDSTLGTTTDKTLAT
                     DKEETYKVDFFSPADKTKAVHTAKVIVGDEKTMMVNLAEGATVIGGSADPVNARKVFD
                     GQLGSETDNISLGWDSKQSIIFKLKEDGLIKYWRFFNDSARNPKTTNKPIQEASLQIF
                     NIKDYNLDNLLENPNKFDDEKYWITVDTYSAQGERATAFSNTLNNITSKYWRVVFDTK
                     GDRYSSPVVPELQILGYPLPNADTIMKTVTIAKGLSQQKDKFSQKMLDELKIKEMALE
                     TSLNSKIFDVTAINANAGVLKDCIEKRQLLKK"
     gene            38621..39505
                     /locus_tag="VC122_000344"
     CDS             38621..39505
                     /locus_tag="VC122_000344"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002995401.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ROK family protein"
                     /protein_id="HEP5640298.1"
                     /translation="MGKLYGSIEAGGTKFVCAVGDEEFTVVDKTQFLTTTPEETIART
                     IAYFKAFEADLAGMAIGSFGPIDIDPSSETYGHITTTPKSGWANVDLLGQLSAAFKIP
                     FDVTTDVNSSAYGEVLARPGVESLVYYTIGTGIGAGAIQHGHFIGGLGHTEAGHTYVM
                     PHPDDMAKGFLGVCPFHKGCLEGMAAGPSIEARTGVRGERLDQEADVWDIQAFYIAQA
                     ALQATMLYRPQVIVFGGGVMTQEHMVLRVHDKFTTLLSGYLPVPDLTDYIVTPAVADN
                     GSATLGNFALAKLAAQGK"
     gene            39600..40553
                     /gene="manA"
                     /locus_tag="VC122_000345"
     CDS             39600..40553
                     /gene="manA"
                     /locus_tag="VC122_000345"
                     /EC_number="5.3.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002983189.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mannose-6-phosphate isomerase, class I"
                     /protein_id="HEP5640299.1"
                     /translation="MSEPLFLKSTMHDRIWGGTKLRDVFAYNIPSDTTGEYWAISAHP
                     NGVSTVTNGRYQGQPLNTLYAQEPALFGNPKEEVFPLLTKILDANDWLSVQVHPDDAY
                     GREHEGELGKTECWYIISAEEGSEIVYGHQAKSKEDLRAMIEAGAWDDLLTRVPVKAG
                     DFFYVPSGTMHAIGKGILILETQQSSDTTYRVYDFDRKDVNGNLRDLHIEKSIDVLTI
                     GKPENSVPATIVLDNMVETTLVSTPFFTVYKWVTSQMVDMKQAAPYLLVSVLKGQGKL
                     YVDQKAYELEKGMHFILPNDVKSWSFDGQLEMIVSHPNQLV"
     gene            40687..43206
                     /gene="secA"
                     /locus_tag="VC122_000346"
     CDS             40687..43206
                     /gene="secA"
                     /locus_tag="VC122_000346"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012560919.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecA"
                     /protein_id="HEP5640300.1"
                     /translation="MANILRKVIENDKGELRKLEKIAKKVESYADQMVSLSDRDLQGK
                     TLEFKERYQKGETLEQLLPEAFAVVREAAKRVLGLFPYRVQIMGGIVLHNGDVPEMRT
                     GEGKTLTATMPVYLNAIAGEGVHVITVNEYLSTRDATEMGEVYSWLGLSVGINLAAKS
                     PAEKREAYNCDITYSTNSEVGFDYLRDNMVVRQEDMVQRPLNFALVDEVDSVLIDEAR
                     TPLIVSGAVSSETNQLYIRADMFVKTLTSVDYVIDVPTKTIGLSDSGIDKAESYFNLS
                     NLYDIENVALTHFIDNALRANYIMLLDIDYVVSEDGEILIVDQFTGRTMEGRRFSDGL
                     HQAIEAKEGVRIQEESKTSASITYQNMFRMYKKLAGMTGTAKTEEEEFREVYNMRIIP
                     IPTNRPIARIDHTDLLYPTLESKFRAVVEDVKTRHAKGQPILVGTVAVETSDLISRKL
                     VEAGIPHEVLNAKNHFKEAQIIMNAGQRGAVTIATNMAGRGTDIKLGEGVRELGGLCV
                     IGTERHESRRIDNQLRGRSGRQGDPGESQFYLSLEDDLMRRFGSDRIKAFLDRMKLDE
                     EDTVIKSGMLGRQVESAQKRVEGNNYDTRKQVLQYDDVMREQREIIYANRRDVITANR
                     DLGPEIKAMIKRTIDRAVDAHARSNRKDAIDAIVTFARTSLVPEESISAKELRGLKDD
                     QIKEKLYQRALAIYDQQLSKLRDQEAIIEFQKVLILMIVDNKWTEHIDALDQLRNAVG
                     LRGYAQNNPVVEYQAEGFKMFQDMIGAIEFDVTRTMMKAQIHEQERERASQRATTAAP
                     QNIQSQQSANTDDLPKVERNEACPCGSGKKFKNCHGRKSFS"
     gene            43322..43678
                     /locus_tag="VC122_000347"
     CDS             43322..43678
                     /locus_tag="VC122_000347"
                     /EC_number="2.7.8.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002983199.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="holo-ACP synthase"
                     /protein_id="HEP5640301.1"
                     /translation="MIVGHGIDLQEISAIEKVYQRNPRFAQKILTEQELAIFESFPYK
                     RRLSYLAGRWAGKEAFAKAIGTGIGRLTFQDIEILNDVRGCPILTKSPFKGNSFISIS
                     HSGNYVQASVILEDKK"
     gene            43675..44775
                     /locus_tag="VC122_000348"
     CDS             43675..44775
                     /locus_tag="VC122_000348"
                     /EC_number="5.1.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002988532.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alanine racemase"
                     /protein_id="HEP5640302.1"
                     /translation="MISSFHRPTVARVNLQAIKENVASVQKHIPLGVKTYAVVKADAY
                     GHGAVQVSKALLPQVDGYCVSNLDEALQLRQAGIDKEILILGVLLPNELELAVANAIT
                     VTIASLDWIALARLEKKECQGLKVHVKVDSGMGRIGLRSSKAVNLLIDSLKELGADVE
                     GIFTHFATADEADDTKFNQQLQFFKKLIAGLEDKPRLVHASNSATSIWHSDTIFNAVR
                     LGIVSYGLNPSGSDLSLPFPLQEALSLESSLVHVKMISAGDTVGYGATYTAKKSEYVG
                     TVPIGYADGWTRNMQGFSVLVDGQFCEIIGRVSMDQLTIRLPKAYPLGTKVTLIGSNQ
                     QKNISTTDIANYRNTINYEVLCLLSDRIPRIY"
     gene            44862..46373
                     /locus_tag="VC122_000349"
     CDS             44862..46373
                     /locus_tag="VC122_000349"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002988534.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CHAP domain-containing protein"
                     /protein_id="HEP5640303.1"
                     /translation="MNKNKLLRVAMLLSLLAPTAESMTVLAQDVMLETHKATTNETSD
                     SSSKEENNKNAAPTTSDKTDQGPLDASAETNSNSLVNADDKKRSDSSQSAVGSSNNKA
                     EAENQVDDKSTDHSKPTDQPKPTDQPKPSPSKVDTAPASSLSRQLPEARTPIQSLSPY
                     VSDLDLSEIDIPSVNTYAAYVEHWSGKNAYTHHLLSRRYGIKADQIDSYLKSTGIAYD
                     STRINGEKLLQWEKKSGLDVRAIVAIAMSESSLGTQGIATLLGANMFGYAAFDLDPTQ
                     ASKFNDDSAIVKMTQDTIIKNKNSNFALQDLKAAKFSRGQLNFASDGGVYFTDTTGSG
                     KRRAQIMEDLDKWIDDHGGTPAIPAELKVQSSASFASVPAGYKLSKSYDVLGYQASSY
                     AWGQCTWYVYNRAKELGYQFDPFMGNGGDWKYKAGYALSKTPKVGYAISFAPGQAGAD
                     GTYGHVSIVEDVRKDGSILISESNCIGLGKISYRTFTAQQAEQLTYVIGKSKN"
     gene            46863..50690
                     /gene="shr"
                     /locus_tag="VC122_000350"
     CDS             46863..50690
                     /gene="shr"
                     /locus_tag="VC122_000350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010922617.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heme-binding protein Shr"
                     /protein_id="HEP5640304.1"
                     /translation="MKKISKCAFVAISALVLIQATQTVKSQEPLVQSQLVTTVALTQD
                     NRLLVEEIGPYASQSAGKEYYKHIEKVIVDNDVYEKSLEGERTFDINYQGIKINANLI
                     KDGKHELTIVNKKDGDILITFIKKGDKVTFISAQKLGTTDHQDSLKKDVLSDKTVPQN
                     QGTQKVVKSGKNTANLSLITKLSQEDGAILFPEIDRYSDNKQIKALTQQITKVTVNGT
                     VYKDLISDSVKDTNGWVSNMTGLHLGTKAFKDGENTIVISSKGFEDVTITVTKKDGQI
                     HFVSAKQKQHVTAEDRQSTKLDVTTLKKAIKEADAIIAKESNKDAVKDLAEKLQVIKD
                     SYKEIKDSKLLADTHRLLKDTIESYQAGEVSINNLTEGTYTLNFKANKENSEESSMLQ
                     GAFDKRAKLVVKADGTMEISMLNTALGQFLIDFSIESKGTYPAAVRKQVGQKDINGSY
                     IRSEFTMPIDDLDKLHKGAVLVSAMGGQESDLNHYDKYTKLDMTFSKTVTKGWSGYQV
                     ETDDKEKGVGTERLEKVLVKLGKDLDGDGKLSKTELEQIRGELRLDHYELTDISLLKH
                     AKNITELHLDGNQITEIPKELFSQMKQLRFLNLRSNHLTYLDKDTFKSNAQLRELYLS
                     SNFIHSLEGGLFQSLHHLEQLDLSKNRIGRLCDNPFEGLSRLTSLGFAENSLEEIPEK
                     ALEPLTSLNFIDLSQNNLALLPKTIEKLRALSTIVASRNHITRIDNISFKNLPKLSVL
                     DLSTNEISNLPNGIFKQNNQLTKLDFFNNLLTQVEESVFPDVETLNLDVKFNQIKSVS
                     PKVRALIGQHKLTPQKHIAKLEASLDGEKIKYHQAFSLLDLYYWEQKTNSAIDKELVS
                     IEEYQQLLQEKGSDTVSLLNDMQVDWSIVIQLQKKASNGQYVTVDEKLLSNDPKDDLT
                     GEFSLKDPGTYRIRKALITKKFATQKEHIYLTSNDILVAKGPHSHQKDLVEKGLRALN
                     QKQLRDGIYYLNASMLKTDLASESMSNKAINHRVTLVVKKGVSYLEVEFRGIKVGKML
                     GYLGELSYFVDGYQRDLAGKPVGRTKKAEVVSYFTDVTGLPLADRYGKNYPKVLRMKL
                     IEQAKKDGLVPLQVFVPIMDAISKGSGLQTVFMRLDWASLTTEKAKVVKETNNPQENS
                     HLTSTDQLKGPQNRQQEKTPTSPPSAATGIANLTDLLAKKATGQSTQETSKTDDTDKA
                     EKLKQLVRDHQTSIEGKTAKDTKTKKSDKKHRSNQQSNGEESSSRYHLIAGLSSFMIV
                     ALGFIIGRKTLFK"
     gene            50887..51762
                     /locus_tag="VC122_000351"
     CDS             50887..51762
                     /locus_tag="VC122_000351"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014407805.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heme-binding protein"
                     /protein_id="HEP5640305.1"
                     /translation="MTKVVIKQLLQVIVVFMISLSTMTGVVYADKGQIYGCIIQRNYR
                     HPISGQIEDSGGEHSFDIGQGMVEGTVYSDAMLEVSDAGKIVLTFRMSLADYSGNYQF
                     WIQPGGTGSFQAVDYNITQKGTDTNGTTLDIAISLPTVNSIIRGSMFVEPMGREVVFY
                     LSASELIQKYSGNMLAQLVTETDNSQNQEVKDNQKPVDTKLGESQDESHTGAMITQNK
                     PKANSSNNKSLSDKKILPSKMGLTTSLELKKEDKFRSKKDLSIMIYYFPTFFLMLGGF
                     AVWVWKKRKKNDKTM"
     gene            51746..52630
                     /gene="isdE"
                     /locus_tag="VC122_000352"
     CDS             51746..52630
                     /gene="isdE"
                     /locus_tag="VC122_000352"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014407804.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heme ABC transporter substrate-binding protein
                     IsdE"
                     /protein_id="HEP5640306.1"
                     /translation="MIKRCKGIGLALMAFFLVACVNQHHKTAKESEQQRIVATSVAVV
                     DICDRLNLDLVGVCDSKLYTLPKRYDAVKRVGLPMNPDIELIASLKPTWILSPNSLQE
                     DLEPKYQKLDTEYGFLNLRSVEGMYQSIDDLGNLFQRQQEAKELRQQYQDYYRAFQAK
                     RKGKKKPKVLILMGLPGSYLVATNQSYVGNLLDLAGGENVYQSDEKEFLSANPEDMLA
                     KEPDLILRTAHAIPDKVKVMFDKEFAENDIWKHFTAVKEGKVYDLDNTLFGMSAKLNY
                     PEALDTLTQLFDHVGDHS"
     gene            52632..53654
                     /locus_tag="VC122_000353"
     CDS             52632..53654
                     /locus_tag="VC122_000353"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014407803.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron ABC transporter permease"
                     /protein_id="HEP5640307.1"
                     /translation="MTASDLQRDQSLLAKKRFRSGLYLVLLILSLLFLAVIALSLGGL
                     AVSYGAIVKGLFVAYDPQVALIYDLRFPRIVIALLAGAGIAVSGVLFQAVLKNPISDP
                     AIIGICSGASFMVLVSSLLLPQLLLYGPIVSFLGGGVSFLLIYGLAWKKGLNPIRLIL
                     TGIAINALFMGLSTALTSFFTSASPMVNALLAGHISQKTWADVGVLFPYTFIGLLLAL
                     LLSKTCNLLLLDDQVIRHLGIDATALRLGISLVAVLLASVATSIVGVVSFLGLIVPHM
                     SRLLVGSKHQILIPFSALLGAFVFLLADTLGRSLAYPLEISPAIIMSIVGGPYFIYLL
                     RRSDII"
     gene            53651..54487
                     /locus_tag="VC122_000354"
     CDS             53651..54487
                     /locus_tag="VC122_000354"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011285106.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEP5640308.1"
                     /translation="MSKNSSHIQVQNLSFAYEEKLVLDDLTFSIPKGKITTIMGANGS
                     GKSTLLQLLTKNLPLKQGQVWLEQEELATISLKAFAKKVAVVHQYHQVVEGLLVGELV
                     AMARLARRSFFKTTTKEDEERLAWALEVTALSDYVYRDLQHLSGGEQQRVWIAMALAQ
                     DTDIIFLDEPTTYLDIKYQLDILKLITKINQELGLTIVLVLHDINQALSLSDHLIGLK
                     DGRLYAQGQPRDLLDQQFIRDVFGVELPFVERNGQQFVLTSLVNETNDIRTQRRDRNK
                     NR"
     gene            54484..56247
                     /locus_tag="VC122_000355"
     CDS             54484..56247
                     /locus_tag="VC122_000355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002983224.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein/permease"
                     /protein_id="HEP5640309.1"
                     /translation="MMETEDTISRGKRKRLLKRLRERIAPKRYLLYVSAFLSWLQFVM
                     RMISFYLIAKTFSTFILGHAIALGRLAGLLLLLNVVGFVLAILGKQLQGIASQFARDS
                     LKQSFFEAFIDLDGQFDAHASDADILTLASQGIDSLDTYYGYYLSLSMRTKWNCTTIM
                     ILVFLIYPLAGLVFLGVLLLIPLSIVAMQKRSKQIMSHYWSSYMDVGNLFMDDLKGLN
                     TLYSYQAIERYEQEFSGKAEQFRKATMSLLGFQLQAVGYMDAVMYLGIGLSGFLAVQA
                     LATGQLSFFNFLFFLLIATEFFTPIREQGYGMHLVMMNTKMADRIFSFLDSVPARKDN
                     KSKTAINFNQIDIQNISLAYEKKTVLSGVTMTLTKGQLTAIAGVSGQGKTSLAQLLLK
                     RQSATTGHILFDGLDSDNLLQETINQQVLYVSDQSTLLNRSIYDNLRLAANLSKKEIL
                     DWIDQHGLLSFINWLPDGLDTIVGENGNLLSPGQKQQVICARALLSKRSLYIFDEATS
                     SLDAENERIIDNLITRLAKTAIVIVITHKMSRLKGANQVLFLNTGQPACLGKPCDLYR
                     DQPTYRHLVDTQARLEASIYG"
     gene            56240..57910
                     /locus_tag="VC122_000356"
     CDS             56240..57910
                     /locus_tag="VC122_000356"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011054954.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEP5640310.1"
                     /translation="MAKPNRSTLSLVWRLLTLMKTLLPWIILAVGFAVMGFVITVSIP
                     TGIAYLGLLAIRQEVIPILALYLLIALAFLRGFVRYGEHYFGHFVAFHSLAAFRNLIF
                     KKLRALSPACLDSQDSGYLLKMIGEDIEALEVFFAHTIAPICTAILSAGLMFWYFCQS
                     SWQLALLALATYACLAIVIPIYFANILQVLLKSQNEGRKDYLSYFLESLRSVKDLLQF
                     QVLDEQFERLIKKSNHVNALDRNVAQAQFLQMALTFFWLGLMILAFSYMVFDGICHDS
                     LSFDKGLLTFIAFTASFSPFLELGRLPLGFKRAMNAARNIFDLLDENVIVDEGTKQID
                     RLRSVSFEDLSFAYPKRQELVFKDLSVTFQEKGIIGIKGESGSGKSTLVKLIMKWYNW
                     KTGDIFLNDRNSCLLNAAKLQDTIAYVPQTAQLFQQSIRENLTFGRQDISDESIWNLA
                     ETCGMKDRLLACKEGLDTIIKSPSDFSAGEGQRLELMRALLKDASCYIFDEPTSNLDS
                     LNEAILLDLIKTHCQGLVFLISHRPSTLACADHLFCVKNGSLKEVNKK"
     gene            57907..58500
                     /locus_tag="VC122_000357"
     CDS             57907..58500
                     /locus_tag="VC122_000357"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014635664.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MptD family putative ECF transporter S
                     component"
                     /protein_id="HEP5640311.1"
                     /translation="MKQLTIKDIMITGAFAALYFICVGLGTFINIFFDRSGNMLYAPA
                     FAALLGGTIYFLLIAKLQTFGPITLLGLMMGGFFFLSGHFFAAMFPSIIFGLIADIIA
                     SLGKYKSRFFNDLSYMVFAFVNSGPIVLMWLLKDAYVESLLARGKSMTYVNRIMYDFS
                     LGHVIWFVGIVLAGALLGSAFAHYLLKKHFIKSGLLS"
     gene            58497..59177
                     /locus_tag="VC122_000358"
     CDS             58497..59177
                     /locus_tag="VC122_000358"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011528920.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="energy-coupling factor transporter transmembrane
                     protein EcfT"
                     /protein_id="HEP5640312.1"
                     /translation="MRLDVRTKLLLLVLANACFFFRVDGFLEFIIVIFLLLLLSALNK
                     KKLAFKLAVVYLLMIGLSVIPLSIFPSYLDHLLSFVSIAGRLVFPSLLAGLITIKTTT
                     IYELVHGLRKWHFPEVWLLTLAVMCRFIPMIRQECCVIHRSLTIRGIILTKWSILIRP
                     KQYLEYLMVPLLLSLIRSSQELTIASLTKGLAVNKGTSECFSSHLTWKDWGVQIWITV
                     IIIITILQ"
     gene            59114..60568
                     /locus_tag="VC122_000359"
     CDS             59114..60568
                     /locus_tag="VC122_000359"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002988553.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEP5640313.1"
                     /translation="MERLGGADLDYCYHNHHDTAMISAEQLVFTYHDQKNPACQVSTC
                     QIASGQFIVLCGPSGSGKSTFLKLLNGIIPDYYAGKYEGRLDVADCQADRDSVETFSR
                     SVASVFQNPASQFFYREVQHELVFPCENQGLDAKVIMKRLWTLAEDFAFAELLNKDMF
                     GLSGGQKQRVAIATAIMQGTNIMLFDEPTANLDSAGIAAVKAYLTQLKAAGKTIIVAE
                     HRLHYLMDLADNFFYFKNGRLTDKLTTQDLLALTDEQRQDMGLRRLDLSDLKPVLAGK
                     IESQHYRPDDSLCIEHLTVRAGSKILRCIEQLSFAAGSISGITGSNGLGKSQLVYYIA
                     GILDDKKATIKFQGIPLSAKQRLSKTSIVLQEVSLQLFAESVSKEVNLGHERHPRTTE
                     VIERLSLTTLLERHPASLSGGEQQRVMIAASLLADKDILIFDEPSSGLDLLQMKALAN
                     LLMQLKTQHKVVILISHDEELLSSVCDTIYQMLP"
     gene            60943..62958
                     /gene="recG"
                     /locus_tag="VC122_000360"
     CDS             60943..62958
                     /gene="recG"
                     /locus_tag="VC122_000360"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014407797.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent DNA helicase RecG"
                     /protein_id="HEP5640314.1"
                     /translation="MILTAPMSNLKGFGPKSAEKFQKLDIYTVEDLLLYYPFRYEDFK
                     SKSVFDLVDGEKAVITGLVVTPANVQYYGFKRNRLSFKLRQGEAVLNVSFFNQPYLAD
                     KIELGQEVAVFGKWDATKSAITGMKVLAQVEDDMQPVYRVAQGISQSTLIKAIKSAFE
                     IDAHLELKENLPATLLEKYRLMGRSQACLAMHFPKDITEYKQALRRIKFEELFYFQMN
                     LQVLKAENRSETNGLPILYSKHAMETKISSLPFILTNAQKRSLDDILSDMSSGAHMNR
                     LLQGDVGSGKTVIAGLSMYAAYTAGFQSALMVPTEILAEQHYISLQELFPDLSIAILT
                     SGMKAAVKRTVLAAIANGSVDMIVGTHALIQDSVQYHKLGLVITDEQHRFGVKQRRIF
                     REKGENPDVLMMTATPIPRTLAITAFGEMDVSIIDELPAGRKPIMTRWVKHEQLGTVL
                     EWVKGELQKDAQVYVISPLIEESEALDLKNAVALHAELSTYFEGIAKVALVHGRMKND
                     EKDAIMQDFKDKKSHILVSTTVIEVGVNVPNATIMIIMDADRFGLSQLHQLRGRVGRG
                     YKQSYAVLVANPKTDSGKKRMTIMTETTDGFVLAESDLKMRGSGEIFGTRQSGIPEFQ
                     VADIVEDYPILEEARKVSAAIVSDPNWIYEKQWQLVAQNIRKKEVYD"
BASE COUNT        19341 a        11034 c        13269 g        19451 t
ORIGIN      
        1 gctttttttg taaaatgttt atgaaaatag agaggagttc attttcaatg gaagaaaaat
       61 tgtttaacaa acattttgtg gccatcacag taataaactt tatagtctat atggtctact
      121 atctttttac ggttattatt gcctttgtgg cgactagaga gctaggagct cagactagcc
      181 aggcaggatt ggcaacaggg atttatattc tagggacttt attagcccgt ttgatttttg
      241 gaaagcagtt ggaggtgttt ggtcgtcgtt tggttttaag aggaggtgct attttttacc
      301 tcttaacgac cttagcctac ttttatatgc caacgattag catgatgtac ttggttcgtt
      361 tcttgaacgg atttggttat ggtgtagtat caactgcgac aaataccatt gtaacagcct
      421 atattccagc tcgtaaaaga ggagaaggaa ttaattttta tggcttgtca accagtctag
      481 cagcagctat tggccctttt gtaggaacct ttatgttgga taatcttcat attgattttc
      541 gcatggttat tgtcttatgt agcgtcttga ttgggtgtgt ggttgttggt gcttttgctt
      601 ttccagtcaa aaacatgtct ttaaatgcgg agcaattggc taaaaccaaa tcgtggactg
      661 tggatagttt cattgagaaa aaagccttgt ttatcactgc aatcgccttt ttaatgggga
      721 ttgcctatgc ttcagtttta ggatttcaaa aattatacac gtctgaaatt catttgacaa
      781 cagtaggcgc ttatttcttt gtggtctatg ccttaatcat taccatcact cgtccagcaa
      841 tgggacgttt aatggatgct aaaggcgata aatgggtctt gtacccaagt tatctttttt
      901 tagcaatggg actcttcttg ctaggtagtg tatcgtctgg aggaagctat ttgctttcag
      961 gagccttgat tggctttggt tatggcactt ttatgtcttg tggtcaagcc gcctccatac
     1021 aaggagtgga tgagcaccgc tttaatacgg ctatgtctac ttatatgatt ggtcttgatt
     1081 taggtttagg tgctgggcct tacctcttgg gcttgattaa agatttggca cttggtagtg
     1141 gtgtggcttc ctttaggcac ttattctggt tagctgctgt gattccactg atttgtacct
     1201 tgctttactt attaaaaaca aagacaagac aggttttgtc ttaaggacaa gaaaaggagt
     1261 ttagtcttat acttaaatac aaacgtatct taacagctgt agatagttcc tatgagccgg
     1321 gattagctgt gggtaaagcc attactatag cgctttgaaa taattctagt ttgctattcg
     1381 tacatgtcaa taaaaatgac tcgcaaaagc cattcgtgaa agagaaaatg tggccttatt
     1441 tttagtaggt acaattggat taagcacgtt tagatacatg acaattggat taacatcaga
     1501 atataggctt tgtcatgtta agattgatct gttgatcgtg agagctaata aaaaatgtta
     1561 taatactatg aaataaagtg ataaagataa ggccttttat ggtccttttc tttaccgctt
     1621 ttattattgt gtgaaaataa cacttaatga tcattttatt tattatttta ttacctttac
     1681 attcatcaat aagcctgtta aaatggtatt tgttatattt tattatttgg aggaaaccag
     1741 aaaacatgga aatgacatgg actgtgaaat acatcacaga atttatcgca acggcttttt
     1801 tgattatttt ggggaatgga gctgttgcta acgtcgactt aaaaggaaca aaaggacata
     1861 actcaggttg gttagtgatt gcttttggct atggcttagg agttatgatg ccagctttga
     1921 tgtttggtaa tgtatctgga aaccatatta atcctgcttt cacagttgga ctagcagtat
     1981 caggcttgtt cccatgggct cacgtcttac aatacgttgt ggcacaatta cttggagcta
     2041 tctttggtca gttggtagtg gtaatggttt ataaacctta ctttatgaaa actgaaaatc
     2101 caaatcatgt tttaggctca ttctcaacga tttcatctct tgataatggt caaaaagaca
     2161 gccataaagt ctcttatatc aatggtttct taaacgaatt tgtaggttca tttgttcttt
     2221 tctttggtgc tttggctttg actaaaaact actttggggt agagttagtt ggtaaattga
     2281 ttgaagcagg ttacgaccaa acaactgcag caacacaaat ttcaccatac gtgacaggct
     2341 cattggcggt tgctcacatt ggtattggtt tcttagttat ggtcttggtt acttcattag
     2401 gtggaccaac tgggccagca ctaaacccag cgcgtgattt tgggcctcgt ttgttacacc
     2461 acttcttacc aaaatcagtt cttggtcaag ccaaaggtga ttcaaaatgg tggtatgcat
     2521 gggttcctgt agttgcacct atcttagcgg cgatagtagc cgtagcagcg tttaaatacc
     2581 tttatatcag ataaaaaaag aactaaagct tcttttagac tcaagacaag ctcgcttaag
     2641 gaacttgtct tttttatatg agttaagcga aatctaatgc aaacttgact gtcttgtata
     2701 gcgtattttg taaattttac tagacttctt gtaaaaaagc ctctcctcag ataagatgac
     2761 taatgcctct aaacggtgtt acaaataaac gaggcatccc taacttcctt tatctggtgt
     2821 tgaaactatt ttgcacggac taaatcactg atagagtaaa aaacgatttt tcatttttgt
     2881 tgtaaaatat taaaaacgtc tctggttagt gattcccaga aacgttatct tatctatttg
     2941 tttgttgctt atgagaggta aaggaggaac gaatcagcat tccaccagtg aagaaaacga
     3001 tagcgatgac cagaagtttc aagctattaa tatctaaatt tgttaagaaa aacacagcta
     3061 gtaaaacgcc aataatacca ccagaaacaa ttccagcaaa accatcccaa ctgacacggt
     3121 ttgctttgat aaactggcta ctagaagctg tcataatcat agctgcatct agcatcataa
     3181 caggcatagc aactgttggg ctaagaccca ttaatgaaaa gaaaatcaat tctggagcat
     3241 aattgcctaa ccccatagtc atcaagactc caacgataaa attaaaacca attccaacaa
     3301 aaagccagat gccatggagg ccatgaatgg tgtctgaaag gtgttctcct ggatggtaga
     3361 ttattctaat gatcatgatt atagctgcta tgattaaaag actaccaagg attcgttgga
     3421 cagttggagc atgccagttt tttgtaatat gagtccccaa ataagctcca ataaaggctg
     3481 ctgcagccat agcaagtaaa gttagaacct caacttttac gacaaaaata aagcaaagag
     3541 attgaatgag caccggaagc acatgagcta cggtcatagt gccaggcaat tgtctatcgt
     3601 cagtgactaa tttagttaac ttaaagcacg ttgtggtagt agcaaaagac cctatcccaa
     3661 gggtgtcaag ggcatctgtt agcaggccaa tccaaaaccc cgtccaaaag cgtttaaaag
     3721 gattgatctt atttttttta agatgtttta ctaacataaa gaaaataaaa agcattgccg
     3781 aaactagcaa agcttgtatc agtctaagga taagcatttc gttcataaag actagtttac
     3841 aaaaaaaaac cttagccgtc aacgtgattt acttcattaa aacatgccac tatgcttaga
     3901 agagctttgg ttcgatgtat tttgttaaaa ttaagctaaa atattaatat tgcatgaaag
     3961 gggacgcctt cctttttccc ttactgagct tagctgttat tgtagtgaat gtttaaaagg
     4021 ctagtttgtc gctctttatc aaaaatctag taggatgaat acgtataatt tcttgtcaaa
     4081 tagaggaatt ttgtctataa tagcagagga ttaggaggaa attatgactt taatggaaaa
     4141 tattaccttt aaaactccag tattacgcgt taatgatcgc gaccttaata ttgcatttta
     4201 tcaaaataat ttagggcttc gtttagtatc tgaagaaaat gcgattgcta tcttttcatc
     4261 ttggggagaa gggcaggaat gttttgtaat tgaagagtca ccttcagtta gaacgcgtgc
     4321 ggtagaaggc cctaaaaagg ttaatacgat tgtcatcaaa acaaatcaac ctaaagaaat
     4381 cgaacaatta ttagctcatg gagcacatta tgacgctctt tttaaaggtc aaaatggcta
     4441 tgcctttgag actatttccc cagaaggaga ccgattcttg ttgcatgctg aacaagatat
     4501 taaacatctc caaggtactg atttaccatc gcttgaaaaa gatgcaactt ttaaaggttt
     4561 aactcaattt aagtttgata ttattgtttt aaatgttatt agtgaagaac gctcaaaagc
     4621 tttttatcgg gatctgttta gtgatcagtt acctattact atggatttta ttcaagaaga
     4681 aggtcccgat ttggcaattg atccccatat tgcttgggat ttagaaattt tagaatttca
     4741 agtctcaaag gactatgata tgaaggtcct aaaagcaact ttagaagaag acgggcacaa
     4801 ggtctacatt gataaaaaac acaaagtatt ggtcctttct gatccaagtc agatcgaagt
     4861 atggtttacg aaatgacttt ggcagtcatt aaatgtattg aaaaccatct tcacaaaaaa
     4921 gtatacaaag gggccagtct ggctctcttt caatctggtc gatggcaaga ataccatatc
     4981 ggaacaattg atggaagacg accagttgat gctaacttag tttatgactt ggctagtgtt
     5041 tcaaaagttg ttggtgtggc cacaatatgc aatattttat tgaataatgg tacgttagca
     5101 ttagatgatc ccttaaaagc atattatcct agtattgttg atgcgactgt cactattcga
     5161 cagttattga ctcataccag tggtcttgat ccttatattc ctaacagaga tgttttaaat
     5221 gcacaacaat taagaaaagc acttaatcat cttactcaaa aaggaaataa aaacttttat
     5281 tacacagatg tcaatttcct cttgttaggc tttatgttag aagagctctt tagtgaatct
     5341 ttagaccaga tatttgacaa gactatcttt actccctttg ggatgtatca tacgtctttt
     5401 ggccctcgtc cagaagctgt accaacatta aagggtgtga gtgatgggga agttcatgat
     5461 cccaaagcaa aaatcttaaa aaaacattct ggatctgcgg ggctattttc aactcttgca
     5521 gatttagaaa gttttagcaa tcattatctt aatgatccat tttcagattg tctgtggcgc
     5581 aactacagcc aacaaacaat tgagcggtca ctagggtgga atttagatgg cgattggatt
     5641 agtcatacag gttacacagg gccgtttcta atgcttaata aaaaagagca aacggcagct
     5701 atttttttga ccaaccgtac ttacgacgag gatgataaaa gtaaatggtt aaaagaacgt
     5761 cagttgcttt acaatgcact aaagcatgat ctgacaacac ccgtatcgta acgtctaaag
     5821 gggcagtttt agcgatctta ttactgtttt taaagtttct tctaaaaaat agccatttca
     5881 tttgtctgat gagattagag tgattataag tgaaaaaaga acagccatct cattttgaat
     5941 caaatgagat ggctgttctt ttttataagt tattgatcat ttagatacta ctatttactt
     6001 gaaatagctt cagcggcatc atccattgaa agcacttcgt ggaagacacg ttgagtcaat
     6061 tcagtttttt gttctggtgt aaggtatttg gtgttgacac agtaaccaga gatacgcacg
     6121 ataacgtctt cgccgttcat gattttgtca taaacatcgt taaggtccat aacgttcaag
     6181 ttaacgtgct gaccaccatt ttcaaagtaa ccgtcaagca ctgttaccaa gttgtcaact
     6241 tgttcgtcaa atgttttacc aagagcacga ggtgatactt gagtagtcaa tgagatacca
     6301 tcgttagcat gtgagaattc cagtttagca agtgagttaa ggttttgtaa ccagccacct
     6361 tttgctttgt tagatgggtt agcacctggt gagaagaatt ccacttggct agtgttaact
     6421 gtaccatctt cgtttaagaa gacgccgcgg tgaactggag agttaccagt ttgtttagag
     6481 taagcaacgt ttgaagtgat ggtaagaagt gatactgaag cctcagcatt cttgtaaagt
     6541 ttgtggcttg caagacgtgt gtggtaagct tccatcaacc atttcgcgat gtcatcaaca
     6601 cggtcgtcat cttcaccgta acgagggaag tcacctgtta cttcgtagtc atagatgtag
     6661 ccattttcat cgcggatagt tttaacagtt gcgtatttaa tagctgacaa ggtatcaact
     6721 gtgttagcga aaccacagat accgaatccc atgttagcac gttggtgtgt tggcaagaag
     6781 gccatttgaa ctgcttcgta gttgtattta tcagtcatgt agtggatgat gttaagcgca
     6841 tctacataag tatctgtcaa ccagtcaaga gatttctcaa agttagccat aacttcgtcg
     6901 tactcaagga cttctgatgt gattggttca acaacgttaa acactttgta gtctctgtga
     6961 acatcatcgt aaccaccgtt aagaccagta agaagggctt tgagaacgtt tacacgagca
     7021 ccgaagtatt ggatgttatg acgttgttct tcattttctg ggtcaagtgg tgatacacaa
     7081 catgaaatac atgacatttc accataacct tctttagcca ttgttgtcac accttcatat
     7141 tggatagaag agtgtttgtg gctcattttc atacagtagc gacggaatgt ttctggcagt
     7201 tggtcagtcc aaagaactgt taagtttggt tctggagagt taccgatatt atcaagagtg
     7261 tttaagaaac ggtagtccat cttagtcaca cggtgacgac cgtcgttacc cataccagcc
     7321 atagaagttg tgatgaatgt tgggtcacct gagtaaaggg catcgtaagc tttagtacga
     7381 ccaaatttta cggtacggag tttaaggaca aaatcatcca caaattcttg gatttctgat
     7441 tctgtaaagg taccgcgagc aaggtcacgt tctgcaaaga tatcaagaac gattggcaca
     7501 cgtccaagag aagtagcagc accgttgatc acacgacata cagccatgaa agcgatgttt
     7561 acccattgga tagcttcttt aacgttttgt gctgggcgac gaacatcaac tccgtaaagg
     7621 tcaccaagtt taaccacttc accaagagct tggtattgaa ggttaacttc ttcgcgaaga
     7681 cgaatggatt cttcatcgat ttcagtgata gcattccagt cattgacttt ttcttgcatc
     7741 aagtagtcag caccgtaaag agcaagacga gcgtaaacac cgatgatacg tccacgtgag
     7801 taagcatctg gaagaccagt tacagtgtgg gcatgacgag cacgacgaat atttgaagta
     7861 taagcgcgga agataccgtc gttaacagtt gtcacgtact tagtaaagat ttcgtgtact
     7921 gctgggtctg gttcatagcc gttttcttta agggtcgttt ccgccatacg gataccacct
     7981 tttggcatga agttcaattt gaacaattca tcgttttgaa taccataaat taattcattt
     8041 tctttatcga tgaaaccagc aggaatgtca gcaattgaag ttggacgagt atcgtatggg
     8101 aaacgtgtag cttcatagtg tgcttttgtt tcttcgataa cttttttgat gtgaagtgaa
     8161 cgttcagtag caccagccaa gaaagattca tcgccatcgt atggggtgta gttagcttgt
     8221 acaaaacgtg agacacttgc tttttctttc cagtcagttc ctttaaagcc ttcccaagct
     8281 ttttcgaaaa catctgtatt ggttttaaca gttgccataa atttctcctt aaattctagt
     8341 aacaaagcga tctgttacat ttacaattct aagtttaaca tataggaaac gttttcccaa
     8401 gcaaaattct tgacaaaatt aagattcttt tataatacag attgttgaaa cagtcacaaa
     8461 ctgtattaga atgtattttt ttggctttag atagcctaaa aggtataata gagaaaggag
     8521 ggacttatgc ttatttttcc actgattaat gacacgtcac gaaaaatcat ccatattgac
     8581 atggatgcct tttttgctgc agttgaggaa agggataacc ctgctttaaa aggaaagcct
     8641 gttgtgattg ggaaagatcc aagagaaaca ggtggtcgcg gagttgtttc cacttgtaat
     8701 tacgaagcga gaaaatatgg cattcattcg gccatgagct ctaaggaagc ttatgagcgt
     8761 tgtcccaaag ccatttttat ttcaggaaat tatgaaaagt atcgaacagt tggggaccag
     8821 atccgccgta tttttaagcg ttatactgat gtggtagagc ccatgtccat tgatgaggcc
     8881 tatctagatg tcaccaacaa taagttgggt ataaaatcag ccgtcaaaat agccaagctg
     8941 attcagcatg atatctggaa agaagtagga ttgacctgtt cagcaggtgt gtcttataac
     9001 aaatttttgg ctaaattggc tagtgatttt gaaaaacccc atggcctcac cctagtcttg
     9061 aaagaagatg ccctgtgctt tttagccaaa ctccccattg aaaagtttca tggtgttggt
     9121 aaaaaatcag ttgaaaaact gcatgacatg gggatttata caggacagga tttgttggca
     9181 gttcctgaaa tgaccttgat tgatcatttt ggtcggtttg gttttgacct ttaccgtaaa
     9241 gcgagaggca tcagcaattc tcctgtcaag tctgatcgga tacgcaagtc aattggcagt
     9301 gagagaacct acgctaaact gctttatcaa gaaacagaca tcaaggcaga gatcagtaaa
     9361 aatgctaagc gcgtggccgc tctcttacaa gaccataaaa agttaggcaa gactattgta
     9421 ctcaaagtgc gttatgctga ttttaccacc ttgacaaaac gtgtcacctt gccagaatta
     9481 accagaaatg ccgcacaaat tgagcaagta gctggggata tttttgacag cttaagcgaa
     9541 aatcctgctg gtatccgcct gttagggata accatgacca atttggagga taaggtagct
     9601 gatatttcct tggacctata ggagaaagag tgctctatcc gtccgcctca ttttggagaa
     9661 aaaagaagct gattagggat ttccctaatc agcttctttg gtccattatt ttattacagc
     9721 atagcttggt ctgtatcatc acctttttcg ttaagggttt tagccttgta aaaagcaaag
     9781 ccataaagag cagcttgtat aaggataagg atagttccaa ccattgacat ggttgttgtg
     9841 aactggttaa ccaagccgat aacagaaagg ataaggccta agatataagg aatataacta
     9901 accgttagac cttgttttaa tttagataag ttttgagcac aaaagacaat gatagcaata
     9961 atagcaagga catttaagat agataagaaa atcataaatg gtgtcatgct gctttgtagc
    10021 tgtgccaatt gctcagttgt taattgggcc cgaaatgctt cattcttgag ttggctttgt
    10081 aagtagaaaa tccctgcaaa tcccattaat gacagtacaa ctccaagccc gtttagaata
    10141 ataatggcaa tagtagacgt tttgagtgcc tgacgcactt tttcataaga aatcatataa
    10201 tcctcctaga gttcttaatc cgaaattgta caatgtgttt aatacaaaaa tattatacca
    10261 tagattaatc aaaaatgtcc gtttttctag gattttttct ggaaaaagag ggcaatatct
    10321 gactctgtaa gccctatcat cgaatcgata gtcgaataat tctcaggtgt taacctaaaa
    10381 tcatcattat ctgactgaga gttctcagtg gggttagagt ttgttgattg agaggaggct
    10441 aagtcggatt gttcttggaa gcgttcgatc aagtaggttt ggcgtttatc gccttcgtgt
    10501 ttaatagcat actcaaaggc agtatattct cccaacagaa ttaacttact tttagaccga
    10561 gtaatggccg tgtaaatcac atttcgttgc aagagtcggc cgctttggcg tgtgataggt
    10621 aaaatcacca cttgaaactc actcccttgc gatttatgaa tgctcatggc ataggccagg
    10681 gttaatttta gccattcatt tctagggtac gtgacttcgc taccgtcaaa atctaagatt
    10741 aattcgtctt gcttagattc ggtgtattta gcaggaatca aatctgtaat ataaccaata
    10801 tctccgttaa agacattcaa ctgagcatcg ttgactaagt gcaggacttt atcaccttta
    10861 cgaaaatgcg tatcattgaa cagaaattct gtttgtcctt gcaagggatt gagaagttct
    10921 tgcatgagtt gattaagatg attgatgcca gcttgtcctt tgtacatagg cgctaaaatt
    10981 tgaatttcct cagcagggat gccactttta atagcagata ggacaatttt ttgaatcatg
    11041 tcagggataa aggctgcttg ggcttcaaaa taagaacggt cagctttttt atcacgaaag
    11101 tcagcagcta agattcctcg acgcatctgg tctgccaaat tgacaatagt agattcttgg
    11161 gattgacgaa atatcttttg caaggcgatt tggggcaggc tattgacttt taaaagatct
    11221 gacaggactt gaccgggtcc aaccgaagga agctggtcac tatctccgac aataatcact
    11281 tgagttgttg aattaatagc tcctaacaac tggttggcaa gccaggtatc caccattgaa
    11341 aattcatcga caatcagcag gtcgcagtct agataatcct ccatggcttg gtaatcgttg
    11401 tcgccattga ggcctaagtg cctgtggatg gttgcgctag gaagtccagt caactcattc
    11461 atgcgacgtg cagcccgacc tgttggagct gctaacaaga taggaaggtc ttttttatcc
    11521 aaatcaatct gatgcaggtt cgcgtaagcc tgcaaaatgc ctcgaataac agttgttttt
    11581 cctgttccgg gaccacctgt taggagaaag accttgctgg ttaaggcctt agtaatggct
    11641 tcttgctgca cttggtcata ggtgatggca aagtcttttt gaacggcttg gatggttgtt
    11701 tgaatggtat catgactaaa tgactgactt aaaggggtat ctaagagacg agagatgttg
    11761 ttagcaatcc cttcttcagc aaaataaagg ctagcatcaa acagtttggt gtcactgttt
    11821 ttgattttgc cctctatgat caactcgctt agttgttcag ccacagcagc aggatcacat
    11881 tctacttgac gtgcatcttc aaggagtgta atggcaaagt ctaataattg tcgtgcttga
    11941 acataagtat ctcctcgatt gatggattct tccaagagac agtgaaggag ggctgcacga
    12001 aaacgtttcg gagagccgct ttcaatccct aagttttcag cgagagcatc tgccatttta
    12061 aacccaaaac cttgtagatc ttcgactaac tgatagggat tttctttaac aaggtcaaga
    12121 gcctcttctt tatacttttc aaacgcttga aaggcaaaac ggttgctaag gccaagttcg
    12181 acgagaccag caatcagttg ctctgtgccg taattcaatt ttagtttagc gacaaaggct
    12241 tgacgattag ccttagatag gccagagata gtttctaatt tgcttgggtc ttctaaaata
    12301 tggtcaatgg tattatgacc atatagtgca atgattttct ccgctgtttt tttaccaatc
    12361 cccttaaaat ggtcgctaga gaaataatta accaaaccag atgaactagg tttgattttt
    12421 tggtaacggc ttagtttgag ttgctgtcca tatttagggt gctgggtcaa ttctccccaa
    12481 aaggtgtagt catctccttc aataatgtca gccatcgttc ctgtgataat gatttcaaag
    12541 tcgtcaatgt cactgtctgt atcctcaatg gcaaggagga gaatcttaaa gaaattagcc
    12601 tggttttcaa aaatgatacg atcaacagta cctgtaaaaa cgtattccat agtaactctt
    12661 tctatttata caagataact gaaaacctgc ccaaaggcag gttttttcta attaaagacg
    12721 gtcattttat tgagtggcca aaaacgagct ttcacttcac cgataaggtt ttcttttttg
    12781 aaactaccaa cttcgcgact atccctggaa acaatacgat catcaccaag aagaaggtat
    12841 tctccttttg gaacactcat ctcaaagcgt gtttgtccct cagaattagt tgtaaaagca
    12901 tcagatgttt ctgctaactg ttggaatagg gtattatagg cgtaagtttt ttggagttta
    12961 tcgtttttaa attgttttag atactcagcc aaatacggct caactgtttt tttaccatta
    13021 atataaagtg tgtcatcatt ataagaaatg gtatcgcctg gcaatccaat aactcttttt
    13081 acgatttctt tcttttgtcc attttcttct tcctgagcaa ctacaatatc aaagcgatca
    13141 atcctagctt gattaaaaac gataaggcgt tcgccatgag ctagagttgg gtccatagaa
    13201 tggccgtcta ctttaacagc ctgccacaaa aaaagacgag atagaccaaa gagaattaaa
    13261 aagagagtga atgggcccca ttctttaata aactgtttca tatgattcct tttatttagt
    13321 gagaagcgct tgcgctttgt gagtgttggc gaagtgaagt ttagcactat attctaagct
    13381 agacatacca taagcggcta agagttggct agcaacttta tctgaagctg aaccagcacc
    13441 gcttgggagt tggtaaccta aatcttgacc taattgatcg agattgtcta aaaatagatt
    13501 tctagcaatg atagagctaa cagccacagc caaatagtga ctttctgcct tttcttgaaa
    13561 ggttagagga tttgggaaat ggtttttttc cttttttaga tgcttttcat aatttgattg
    13621 gctagtgaaa gcatcaataa caatttgttt aggttgaatg cctttttgta acaacaaaaa
    13681 aatagcttgg ttatgaagag caactttgat agaaatggta ttatatggtt tacttttacc
    13741 aaccaattcg ttatattttt taggtgatag gagaagtgat tgatgaggta tttgtttctc
    13801 taacagaggc gcaatctgtt gaatggtttt atccgataat tttttagaat catcaacccc
    13861 aagctttttc aggaagggat gatctttagg atcgacaaaa cttgccacaa cagcaatccc
    13921 accaaaataa gagccattac ccacctcatc actgccgatt ataggtatat cttggctatt
    13981 gttgcttgct tcaaaaactg tcttagctac tggaagatta agctcttgag ctaaagcatt
    14041 tgcaccgttt ccctggagaa ctaattttcc ggatttgtac agcaaaacag taaccccatt
    14101 ttttttagca gcaaaggcta catagatatt ttggctactg atggtgtagg gggctagctg
    14161 tttttttaaa tgtttactca gtatagcatc tattttcaaa actaaggtat tcataataat
    14221 tattttaaca taaaatagtt gactatggtt aattcataag tttatttaac aaagaaaaca
    14281 gagactcgaa tgtttagacg ggttagggca atggtgttga ctattttacg aaagtttcat
    14341 tttacggtat aatgctaatg aggtgaccct atgaaaagta ttaatcgcta taaattcacc
    14401 tttggtgaaa aaacattaac attgacaact gacaaagaca atctctttat ggaagaagtt
    14461 gagcgggtag ctaaagaaaa gtatcaggct cttaaaaatc atttaccaga agctgatgac
    14521 gagacaatcg ctattttaat ggctatcaat accttgtcaa cacaattaag cagagagatt
    14581 gccattgaaa aaatggaagc tgaaatttta gatttacgtc aaaaaacctt ggttggtctt
    14641 caagaaaaag ctaatcaaat agatgtgaat gaggtttaat catgttatct ttattgatag
    14701 tgcttgtttt aacttggaat ttttatattg gttatagtag aggtattatt cttcaatcgt
    14761 tttatgtctt aggagcccta ctttctcttt tggtagctaa tcgattttat attggattag
    14821 cccataagct aacgctttgg attccttatt ctaacccggt agaaggaaca tcagtctttt
    14881 tctttaaatc agttgatatc tttgttttag ataaggttta ttatgcaggc cttgcctttt
    14941 ttatcatttt cttattgggg tatgcactga gtcgctttct tgggattttt gttcatttcc
    15001 ttttgctaga ttattttgat aaccagtgga caaaatgttt gagtggcgga ctagcgcttc
    15061 tagtatcgct tctttttttg aacatgttac tgtctatttt tgcaacggtc cctatgcctt
    15121 ttttacagca ctatttgcac tctagttttc ttgctcgttt ggtgattgaa caccttccac
    15181 cattgacaat aattattcaa aaattgtgga ttcaagccat tatttgatat cgttttgtga
    15241 ctaatataag aacctggagt gagtttcacc tttaggttct ttatttacga gaaactgttt
    15301 agaaataaag agaacgcatg ggaacttagt gactttcttg ccacattttc catcatttta
    15361 ggtataatga agagtatgaa taacaagatt ttagagcagt tagaatttaa caaagttaag
    15421 gaattgctat taccttatct caagacagaa caatcacaag aagaattatt agagctggag
    15481 ccgatgacgg aggctcctaa aatagaaaaa agttttaatg aaatttctga catggaacag
    15541 atttttgttg aacatcactc atttggcata gtcagcctaa gttcaatctc tgagagttta
    15601 aaacgcttag agctttcagc tgatcttaat attcaagaac ttttggctat caaaaaagtt
    15661 ttacagagtt cttcggatat gattcacttt tattctgatt tggataatgt ttctttccaa
    15721 tctttggatc gtttgtttga aaatttggaa caattcccta atctgcaagg gtcttttcaa
    15781 gctatcaatg atggtggttt tttagaacat tttgcgagtc cagaattaga gcgtatccgt
    15841 cgtcaattaa caaatagtga acgacgggtt cgtcagattt tacaggatat gcttaaggaa
    15901 aaagcagagc ttttatcaga gaatctaatc gctagtcgta gtggacgaag tgtcctacca
    15961 gtaaaaaata cttatcggaa tcgtatttct ggtgtggttc atgacatctc ttcttcagga
    16021 agtactgttt atattgagcc tcgtgctgta gttacactaa acgaagagat aactcagctt
    16081 agagctgacg aacgccacga agaaggtcgt attttacacg cattttcaga cttgttaaga
    16141 cctcatgtcg ccactattag aaataatgca tggattcttg ggcatcttga ttttgtaagg
    16201 gctaaatatc tttttatgtc tgataataag gcgacgatac ctaagatctc tgatgacagc
    16261 acgttagcat taatcaatgt tcgtcatcct cttttaagta atcctgtggc taatgactta
    16321 cattttgatc acgatttaac tgcaattgtc atcactggtc ccaatactgg tggtaagacg
    16381 attatgctaa aaacactcgg tttagcacaa ttaatgggac agtctggttt gccagtatta
    16441 gcggataaag gtagtaaaat tgcagtattt aacaatatct ttgcagatat tggcgatgag
    16501 caatctattg aacaaagtct atcaactttt tctagtcata tgacgcacat agtcagtatt
    16561 ttaaacgagg ctgaccgcaa tagtttagtt ctctttgatg aactaggagc aggaacggat
    16621 cctcaagaag gtgctagttt ggctatggct attttagaac accttaggtt aagtcatatc
    16681 aaaacgatgg cgaccacgca ctatccagaa ttaaaagctt atgggattga gacaaatttt
    16741 gtagagaatg cgagcatgga atttgatgcc gaaacgctta gccccacgta tcgctttatg
    16801 caaggagttc ctggacgatc aaatgcattt gaaattgctt ctcgccttgg tttagctcca
    16861 tttattgtta aacaagctaa gcagatgaca gattctgact cagatgttaa ccgtattatt
    16921 gaacagttag aggcacagac acttgagaca cgtagaagac tggatcatat taaagaagtt
    16981 gaacaagaaa atctcaaatt caatcgtgcg gttaagaaac tctataatga attttcacat
    17041 gaacgcgata aagagttaga aaaaatctat caagaagctc aagaaattgt agatatggct
    17101 ttgaatgaga gtgatactat cttaaaaaaa ctcaatgata agagccaatt aaaacctcac
    17161 gaaattatag atgctaaggc acaaataaaa aaattagcac ctcaagttga tttatcaaaa
    17221 aataaagtct taaataaggc taaaaaaatc aaagcagctc gtgcccctag aattggtgat
    17281 gatattatag tgactagtta tggacagcga ggtaccttaa ctagtcaatt aaaagatggc
    17341 cgttgggaag cacaagtggg aattatcaaa atgacattaa cacaagatga atttagcctc
    17401 gttagagtcc aagaagaaca gaaagtcaaa aataaacaga ttaatgtggt taaaaaggct
    17461 gatagttctg gaccaagagc tcgacttgat cttagaggta aaagatacga agaagcaatg
    17521 caagagttag atcattttat tgatcaagca ttgcttaaca atatgggaca agttgatatc
    17581 attcatggta ttggtacagg cgttattcgt gagggagtga caaaatatct tcgtcgtcat
    17641 aagcatgtta agcattttgc ttatgcctca caaaatgcag ggggatctgg cgccacaatt
    17701 gtaacgttag ggtaaatgat ggaatcatat gaacactttt atgccaaact atctcaacct
    17761 tttagaaaaa aaccacagtt aataattctt ttaaatgttt tactaaaaat aatcacagga
    17821 gtgatgtaca tactttatcc atcttttctg atatttacgc tttggcaagg gatgacattt
    17881 caattatggt taaggttatt aattattcct gctgttggct ttatagcttt gtcttacatt
    17941 aggaaacgct ttgattttcc acgaccgtat gaaaaatgga atattaaacc tttaattgat
    18001 aaggatacaa agggaaggtc aatgcctagc agacatgttt tttcagctac gatgattagt
    18061 atgtgtttgt tacgatatta tgtttacttt ggaatagttt gtttaatatt atcggtttta
    18121 ttggcaattt gccgtgtgat agcaggtatt cactatccta aggatgttat tgtaggttac
    18181 cttattggct tgatgttagg gctttgttta ttgatttagc gagttcgtag caaatagttt
    18241 caaaaacaac taaattcttg tacaataggc ttatcattaa gatgaaatgg agaaaaaaga
    18301 tggcattaga agtaacagat gctacatttg tagaagaaac taaagaaggt ttagtactca
    18361 ttgatttttg ggcaacttgg tgtggtccat gtcgtatgca agcaccaatc ttagaacagt
    18421 tatctcaaga aatcgatgaa gatgaactca aaattttgaa aatggatgtt gatgaaaatc
    18481 cggaaactgc tcgtcagttt ggtattatgt caattccaac attgatgttt aaaaaagacg
    18541 gagaagtggt taaacaagtt gcaggtgttc atactaaaga ccaactgaaa gctattattg
    18601 ctgaattgag ttaactaaaa taaagcccag cgtcacgctg agctttattt tatgacctac
    18661 taacttcaat ggaataacca tgtaaacata aaacgacttg caaaagcaaa agcaacagta
    18721 atgaggaaaa atacagtgac aataaggggt ttctgataag gaagttttgc tctttcttct
    18781 cgtttttcga tttcgtctaa taattttcca tttgaaatag ctaggatttc cttataaggt
    18841 tgcacatcat attttttctt aaaacgttcc acacgattag cataagtcat agtaattatc
    18901 gataataaaa caaaaatgcc aagtcctagc cactctaaga aatagactta atggataact
    18961 tgaaatcatt aatagtatca tcaatgttac cattactttg gtatctcgtt taaaattctt
    19021 tttatcaacc tgagtgatgg tatatttcat tttctcaatg tctcctttaa tgagttggtc
    19081 taagctcact tgaaaaattt gacttaggag taagaggcta tggatgtctg ggtaagtttt
    19141 gtcattttcc cagttagata tgctctgacg gcttacatag actttctcgg ctaatttttc
    19201 ttgcgagagc gcttgttgtt tacggtagcg aataatttgc tgaccaattt ccataaatat
    19261 caccgaaaaa ctttacagcg gcgtaaagga attgtaaaca aaaagtgaac cgttatagtt
    19321 cacttgcgat taagcatttt tctgctttaa gtaggtctct agcattttct tttgcggtgt
    19381 tgcaaaggga tacagagcaa agtcctcaat tgctacccac tttaggtgag gagcattagg
    19441 taaatctgtt gctttgacca ccccctctat gagttctatt gtccacttct ggtgactaaa
    19501 agtgtgctta atatttggaa agtgattatc agtccattgg ggctttaatt gatattctct
    19561 ttcgaaagtt tcattttggg tttgccagat tatggggttg gattgattgt catcaaataa
    19621 atctaattgt tgagaaagag gtgacgtttc gataatagga aatgaccaaa aacctcctag
    19681 aagtctccct ttagtatttt tctccagtag atattggcca ttttggttac gaatgacaaa
    19741 agcctgaatt cgcattggtt taggcttttt ctttggattt tttatggggt atttaccata
    19801 agtaccattc aaataagcag cgttaaagaa acgaataggg ctttcatcag gcctaggtgt
    19861 cttagcggat tcgatgtctg tacctagatc cataagtgcc tggttaaaat caccgggtct
    19921 gtcagggtca attagtattt ccataatagc ttgaaaaatt ttacgatttt tagggtcacc
    19981 gatatcataa ttaacttcaa acaaacgagc catgacacgc atcacattgc catcaacagc
    20041 aggctctggt aagttgaatg agatgctggc gatagcacct gcagtataag gaccaatccc
    20101 ttttaatgaa gcaatatcat cataagtgtg aggaaatatg ccaccaaaat caaccatcac
    20161 ttgctgtgca gccttttgca tattacgcac ccgagaataa tagcctagtc cctcccatgc
    20221 ctttagtaat tgttcttcag gagcgtctgc caaatctttt atttgaggaa accactctag
    20281 aaaacgttta taataaggga ttaccgtatt aacttgtgtt tgttgcaaca tgatttcaga
    20341 aacccaaata taatatggat tagtagttct tcgccaaggg agatcacgtt tttcttggtc
    20401 ataccaccct aaaagtgtgc gacgaaaaga agcgattgtc tcattatccc acatattaat
    20461 accataatct tttaattcaa tcatgttgtt attgtaacac aggagcctta aaaagagtaa
    20521 aaaaagattt taggtatttg cgaaaaagaa aaggaaatat tataataata ttaggtttac
    20581 ctcatctaaa tgttgattta gaataactga aattaacgat agaagcagac aggttttaat
    20641 ttggagggga catgttccag aaattaaaga atcttattga tcggtattcg tggttcgttt
    20701 ttataatgat gattcttgtg ttatttctta actatattta tccatttgat ccttctttga
    20761 gcggactaca gttggaatgg ctgatcatga cgcaagtttt tcctggatta ggtatcgtat
    20821 gtgccatggt tgctattatt tttaaaaagt ttaacctttt tgttttggcc ctaattttat
    20881 attttgcact ttggttaaaa cttttccttg ctttttcttt tttcacctat tttttaggag
    20941 tctgactaag tgccaatttt taaaactcgt gaaaggaaat gtttgacaaa acttagtcaa
    21001 gtagaaaaag ttccttgctt ttaattgact attagtgtac aataagattc tgtgagtaca
    21061 cctcacttac tcactccaaa agtagtgagt cattagacca aaaggaggaa aatatcaatg
    21121 gctaaatacg aaattcttta tatcattcgt ccaaacattg aagaagaagc taaaaacgct
    21181 ttggtagcac gctttgactc tatcttgact gacaacggtg caactgttgt tgaatcaaaa
    21241 gattgggaaa aacgtcgtct tgcatacgaa atcaacgatt tccgtgaagg actttaccac
    21301 atcgttaacc ttgaagcgac tgacgcagca gctcttaacg agtttgaccg tctttcaaaa
    21361 atcaatggtg acattcttcg tcacatgatc gttaaacttg acgcttaatt ccaactaaga
    21421 gggtagttta tgattaataa tgtagtacta gttggtcgta tgaccaagga tgcagaactt
    21481 cgttacacac caagtcaagt agctgtggct accttcacac ttgctgttaa ccgtaccttt
    21541 aaaagccaaa atggtgaacg cgaggcagat ttcattaact gtgtgatctg gcgtcaaccg
    21601 gctgaaaatt tagcgaactg ggctaaaaaa ggtgccttga tcggagttac gggtcgtatt
    21661 cagacacgta actacgaaaa ccaacaagga caacgtgtct atgtaacaga agttgttgca
    21721 gataatttcc aaatgttgga aagtcgtgct acacgtgaag gtggctcaac tggctcattt
    21781 aatggtggtt ttaacaataa cacttcatca tcaaacagtt actcagcgcc tgcacaacaa
    21841 acgcctaact ttggaagaga tgatagccca tttgggaact caaacccgat ggatatctca
    21901 gatgacgatc ttccattcta gtcagaatgg attgaacgaa tacgaaatta tttgagttcg
    21961 ggctaaaaat ttagaactta gaatttagat caactcccta ggaaatcaag atttcttgcc
    22021 agttttctaa aattcagtcg ttttttaaga gcccttaaca tcataattta taaaggagaa
    22081 aatacatggc tcaacaacgt cgtggcggat tcaaacgccg taaaaaagtt gacttcatcg
    22141 cagctaacaa aattgaatat gttgattaca aagatactga gcttcttagc cgtttcgttt
    22201 cagaacgtgg aaaaattctt cctcgtcgtg taacaggaac ttcagctaaa aaccaacgta
    22261 aagtaacaac agcaatcaaa cgtgctcgcg ttatggctct tatgccttac gtaaacgaag
    22321 actaataaac aaacagtcca tttcggactg ttttttagtt gactaggact agtctaggcg
    22381 tataggtgag ttttttctaa aagagagaat gatagtaaac gaaaatcctt gcgtagacag
    22441 caaggatgaa atgattgtta tttgttaaca ggtgacatag tgttttgaat gttgtatttg
    22501 cgttgtaggt aatagcgtaa agcaagggaa ataccaccaa taatgagcaa ggcaagagga
    22561 ggcaactgtg ggttaagaga ggttggtaaa aaggcagtcg cagagtacaa ggcaatccaa
    22621 atcaacatgg ctaatgataa ggctgcgatg attttgaacc aacttggacg aaggctctta
    22681 tcttttccga gatgacggta aataaagtaa taagtagcgt acatggatgc tcctccacca
    22741 aaaccaagtg ccagtaagga aatcaatcct gtaactgtag cgtttgtgtt gaagaaggtc
    22801 atgatgccat ttaaaagggc aacaattccg atgaatagca aagaggtatc tagccacatc
    22861 aaccaaggat tggtgttttt gtcagtttct gctgcttttt gactagggtc ttggctaaaa
    22921 gaagctgccc aagcagtcgg agtgcctaat agatttcgag cggtgacacc ttttttctgg
    22981 ttttctagaa tagctggaat ggcttcttcg agaatcagct taatgtcttc atcagacttg
    23041 ccgtcttgga ttaatttgtt cgtagcggtg tggataaatt cttgattttt tttggtaagt
    23101 tcttgtaatt ccatgggaat cccttcttta tatgggttag ttaaagtaag tcaatgatat
    23161 tagctagtgt caaatgagac tttggagcca gccgtttgac acagagtttt gtcaggtgtt
    23221 aactacattc tacagcttgc tccactctag aaccattttt ttctaataaa gtaaattaca
    23281 acaaaggaac taagtagcat agcaatcaag gtaatatacc aaaaagcatg ttctaggcca
    23341 tttagtggta accagttgtt ttggaaattc ataccatagg cggagaaaat cacagttggt
    23401 atatctaaag ccatggtcat caaggccagt gttttcataa tagtgttctg gttgttgttg
    23461 ataatagaag cagtggtttc agtcatggca ttcaagacat tttcataaat gccagccatc
    23521 tcaatggcct gttgggtttc aatcaaggta tcttctagca aatcctcgtc ctcaatgtat
    23581 ttcttgagag agcttgttga gctggataat ttcttgacga tgcgttcgtt gaatttcaag
    23641 gaagccttta agtagacaat ggatttttca agttccatca tgtcgataag ttcttcattt
    23701 cgagtagctg cttcaagctg agcttccaaa cgttcgcttt ggcgatcaat ggtcctcaag
    23761 gcagtcagaa agagctcggc gttgcgatat agaatctgaa aaacaaaacg tgtcttcatg
    23821 aaggtataga aatttttgac ccggcggttg tgaaaatgat cgaaaagggt catgtcatgc
    23881 agacaagttg tgataacagc attttccgtt acaataatgc ctaaaggcat agtaatatag
    23941 taacttttat tgttacgttc ttcgtatatc gggacgtcta cgataattag ggtataatca
    24001 tcttctacgg caatacgtga ggtttcctcc acgtccaaag gggcgcgcaa gtcagaaata
    24061 tcaatgttaa attgatcggc gatcttcata gactcttctt gggaagggtt aaccagttta
    24121 atccaagctc ctggctcgaa tgtttctatt tctttgaatt caattgccga tgaaagaaac
    24181 atttgtttca tgacaagccc tcctttttga tgattgactg gtagagattg acatcaaacc
    24241 ttgataataa agccttctca aggattttct gaaaaattct caatacttat ctattatacc
    24301 aaaattcgca aagtttgggg caataggtta tcagaaattt ttgttataat ataagaaagc
    24361 tcgatgagga gggtgccttt aggcacattc aagatgcaaa ataaaataat aattcatggg
    24421 gcaagagccc acaatttaaa aaatattgac gtggaaattc cacgagacaa attagttgtc
    24481 gtgacagggt tatcagggtc aggaaaatct agtctagcct ttgatactat ttatgcggaa
    24541 gggcaacgtc gttacgtaga aagtttatca gcttatgcta gacagttctt gggtaatatg
    24601 gaaaaaccag atgtggattc cattgacggt cttagccctg ctatttccat tgatcaaaaa
    24661 actaccagta agaacccccg ctcaacggtt ggtacggtta cggaaatcaa tgattattta
    24721 cgtttactat atgcgcgtgt gggcacacct tactgtatta atgggcatgg ggccattaca
    24781 gcatcttctg cagaacaaat tgtagagcag gtcttagccc tccctgaacg gacacgcatg
    24841 cagattttag cgcctattgt tcgccggaaa aaaggtcaac acaaaaccat ttttgagaaa
    24901 attcaaaaag atggttatgt ccgtgtgcgg gttgacgggg atatttttga cgtgacagaa
    24961 gtgccggagc tgtccaaaag taagatgcac aacatcgagg tggttattga ccgtctggtt
    25021 aataaggatg gtattcgtag ccgtttattt gattcggttg aagcagcttt aaggctggga
    25081 gatggctacc ttatgattga cactatggat ggcaatgaac tgctcttttc tgagcattat
    25141 tcctgtcctg tctgtgggtt tacggttcct gaattagaac caagactctt ctcttttaat
    25201 gcgccgttcg gctcttgtcc gacctgtgat gggctaggca ttaagttgga agtggatttg
    25261 gacttggttg ttcctgaccc aagtaagtct ttgagagagg gagctttggc gccttggaat
    25321 cctatttcct ccaattatta cccaaccatg cttgagcaag ctatggcaag ttttggggtg
    25381 gatatggata caccttttga ggccttgact gaggaagaaa gagatttagt gctttatggt
    25441 tcaggagata gagaatttca ttttcactat gtgaatgatt ttggtggtga gcgtaacatt
    25501 gatattcctt ttgaaggtgt tgtgactaat gttaatcgtc gttatcacga aactaatagt
    25561 gattatacgc gcaatgtgat gcgtggttat atgaacgaat tgacctgtgc gacttgtcat
    25621 ggttaccgtt tgaatgacca agccttgtgt gtgcatgtgg gtggcgaaga aggacctcat
    25681 attggtcaaa tttcagaatt atcgattgcc gaccacttgc agttgttgga agaattggaa
    25741 ctcacagaaa atgaaagtac cattgctaaa ccgattgtca aagaaattca tgaccgcctg
    25801 accttcttaa ataatgtggg actgaattat ctgaccttgt cacgggctgc tgggacttta
    25861 tcaggtggtg aaagtcaacg aatcaggtta gcgacacaaa ttggttctaa cttatcaggg
    25921 gtgatctata tcttggatga gccttctatt ggattgcacc aaagagacaa tgaccgtttg
    25981 atcgaaagcc tgaaaaaaat gcgtgatttg ggtaatacct taattgtggt agaacacgat
    26041 gaagacacca tgatgcaggc agactggctg attgatgtgg ggcctggggc aggtgagttc
    26101 ggtggagaaa ttattgcctc tggaacgccg aaacaagtgg ctaagaataa aaaatccatc
    26161 acgggtcaat atttgtctgg taaaaaattc attccagtgc cattagagcg tcgttctggt
    26221 aatgggcgat tcattgaaat aaaaggagca gcgcagaata atcttcaaag cctcgacgtg
    26281 agattcccac tgggtaaatt tattgcggtc acaggggtat caggctcagg gaaatcaacc
    26341 ttggttaata gtattttgaa aaaggctgtc gcccaaaaat taaaccgtaa tgctgataag
    26401 cctggtaaat atcactctat ttctggtatt gaacatattg aacgcttgat tgacattgac
    26461 caaagtccga ttggaaggac gccacggtca aatccagcca cctatacggg tgtctttgat
    26521 gatattcgag acttgtttgc ccaaaccaac gaagctaaga ttcgtggcta caaaaaaggc
    26581 cgtttctctt ttaatgtcaa aggtgggcgt tgtgaggctt gttcaggtga tgggattatc
    26641 aaaatcgaaa tgcacttttt gccagatgtt tatgtgcctt gtgaggtttg ccacggcaga
    26701 cggtataatt ctgagacgct tgaggtgcat tataaagaaa aaaatattgc tgaagtactt
    26761 gacatgactg tcgatgatgc cttggtattt ttctctgcta tccctaaaat tgcccgaaaa
    26821 attcaaacca ttaaggatgt tggtttgggt tatgtgacct tgggtcaacc agctactact
    26881 ctatctggag gagaagcgca gcggatgaaa ttagctagtg aattgcacaa acgctcgaca
    26941 ggtaagagct tgtatatcct agatgagcca acgactggtt tacacacaga tgatattgca
    27001 aggctattaa aagttttgga acgctttgta gacgatggta atacggttct tgtgattgag
    27061 cataatttgg acgttatcaa atctgctgac catattattg acttaggacc agaaggtggt
    27121 gtcggaggtg gtcagattgt tgccacgggg acaccagaag aggtagctca agttaaagaa
    27181 agctatactg gtcattattt gaaagtaaaa ctacaacaat aggattagga gttcgtttga
    27241 gctccttttt aatcttttaa atgctctcaa atcatggtaa acttaaagta gttttttcat
    27301 gctacaatat tctcaaaagt taccaaagga gttcgcgatg tcaggatttt tagagcaacg
    27361 attaggtcac tgcctaaggc agatggcaga gaaggggcta gaggctcttc tagtcaccca
    27421 tttaaccaat agttattact tgacaggttt ttctggaact gcagcaactg ttttgataac
    27481 ggccaaacgt cgtgttttga tcacagattc acgttatacc ttgcttgcta aagctagtgt
    27541 tgagggattt gatattatcg aaagccgcac gccgcttaag gttgtggcag aattgttaga
    27601 ggctgatcaa atagattgcc ttggttttga ggaccaggta tcgttttctt tttatcaggc
    27661 catgcaagca gaactgtcag gaataacctt gcttgctcag tcaggttttg tggagcattt
    27721 acgttttatt aaggatactt ctgaaattga taccattgct aaagcgtgct cgatctcaga
    27781 caaagcattt gaagatgctc ttgattttat taaaccaggg acaaccactg aacgtgacct
    27841 ggctaatttt ttagattttc gtatgcgtca gtatggtgcc agcggcacat catttgatat
    27901 cattgtagct tcaggctatc gctctgccat gcctcatgga cgcgccagtg acaaggttat
    27961 ccagaataaa gagagcttga ccatggactt tgggtgttac tacaatcact atgttagtga
    28021 tatgacgagg accattcata ttggccaagt tactgatgaa gaacgtgaga tttatgctct
    28081 tgttcttgct gctaataagg ctctcattga taaagctagc gctggtatga cttatagcga
    28141 ttttgacggt attccgcgcc aactcatcac tgaggctggt tatggtagtc acttcacaca
    28201 tggcattggt catggtatcg ggcttgacat ccatgagaat ccgttttttg gtaaatctga
    28261 gcaacttctt caagcaggaa tggtggtaac agacgagcca ggaatctatc tggataataa
    28321 atatggcgtc cgtattgaag atgacttggt catcacagaa actggctgtc aagtattgac
    28381 cttggcacca aaggaactga ttgtgttgta aattatctta gggaaggatt agaaggacga
    28441 agcaatgaca aatcgtttat catggcaaga ttactttatg gccaatgcag agcttatttc
    28501 caaacgctct acctgtgacc gcgcctttgt gggagctgtt ttggtaaagg ataatcgtat
    28561 tattgccacg ggttataatg gtggtgtttc tgccacagat aattgtaatg aagcgggcca
    28621 ttatatggaa gatggacatt gcatcaggac agttcatgct gaaatgaatg ctttgattca
    28681 atgtgcgaaa gaagggattt cgactgacgg aactgaaatt tatgtaacgc actttccctg
    28741 tatcaactgt accaaggctc tgttgcaggc tgggattaaa aagattactt acaaagcaca
    28801 ttatcgtccg cacccgtttg cgattgaact aatggagaaa aagggagttg cgtacgttca
    28861 acacgacgtg cctcacatca ttttgggtgg taaggaagga cctaatggtt agcttgggcg
    28921 aatggtcata aaagatttga gcctagggcg cttttgtggt aaaatgaagt gataatattt
    28981 tagtaagagg taaataataa tgattgaagc aagtaagctt aaagccggta tgacattcga
    29041 atcagaaggg aaattaattc gtgtccttga agctagccac cataaaccag gtaaaggaaa
    29101 cacaattatg cgtatgaaat tgcgtgatgt gcgtacaggg tctacctttg acacaactta
    29161 ccgtccagat gaaaaatttg agcaagctat cattgaaact gttccagcac aatacctata
    29221 taaaatggat gacactgctt acttcatgaa tactgacact tacgatcagt acgaaattcc
    29281 agttgctaac gttgagcaag agttgcttta cattcttgaa aactcagacg ttaaaatcca
    29341 attctatgga agcgaagtga ttggagtgac tgttccaaca accgttgaat tgactgttgc
    29401 cgaaacacaa ccatctatca aaggtgcaac tgtcacaggt tctggtaaac ctgcaactct
    29461 tgaaacaggt cttgtggtca acgttccaga ctttatcgaa gctggtcaaa aactaatcat
    29521 caacactgca gaaggtactt acgtttctcg tgcttaatcc tttgtcagaa caggttagtc
    29581 taaagaagag aaaggaaatg ctatgacaac tgaacatatc ggtgaaatcg ttatttcacc
    29641 acgagtactt gaggttatta ctgggattgc taccacacaa gttgagggcg ttcattccct
    29701 tcataacaaa aaaatggcag atagctttaa caaagcaagt cttggtaaag gggtttatct
    29761 ccagacagaa gaagatggaa gtgttacagc agatatttat gtttacttgc aatatggagt
    29821 taaggtcccg actgtatcta tgaatattca aaaaacagtc aaatctgctg tgtacgatat
    29881 ggcagaagtg cctatttctg cagttaatat ccatgttgaa ggtattgttg cggaaaagac
    29941 accaaaacca gatttgaagt cactttttga tgaggatttc ttggatgact aacagctttc
    30001 aaaattcaag aagagatttg cgtgaacgtg cctttcaggc tttatttaac atagagatgg
    30061 gagctgagtt attggcagct tctcagtttg cctatggtta tgataaagta acaggggagg
    30121 acgctcaagt attggagctg ccgatttttt tattaagtct tgtgacgggt gtcaataatc
    30181 ataaagaaga gttggataac ttgatatcaa ctcatttgaa aaaaggttgg tcgttagaac
    30241 gcttgacact aacagacaaa acgttattac gactaggtct ttttgaaatc aagtattttg
    30301 acgaaacacc agaccgtgtt gcactaaacg aaatcattga agtagctaaa aaatattctg
    30361 atgaaacctc tgctaaattt attaatggct tacttagcca atatgtctcg gaagctcctt
    30421 cagcaaataa atcataataa gtcggagcaa tcttaggatt gtatccgatt ttttcaattt
    30481 atgtacgaat attaaaagac aaacaagcat actctcgcat ctagaaagag tatgcttgtt
    30541 tgtcttttag agtaattagt gattggttat gttttgttta aatacttgct cctggaagta
    30601 gcgtaatcgg taaaatatag tctttacttg ttttttcttt tttaattttt ttgatgagta
    30661 tttcgacact caatttagca atctcatcaa taggttgacg aatggtcgct aattgaggaa
    30721 catattgctg gataaatgtc gttccatcat aacctatgac tttcatatcc tctggaatag
    30781 taatgtgaag ttgtttggct actttcataa tgaggatagc agttaaatca tcagaaacga
    30841 aaaggccatc aggttttcgg gttgccaaaa tggatttaat ttccatttcg cgtctaacag
    30901 gagataggtt atttggtaat ttgatgattt ctgcacttcg tttgagctgg tagttaaacc
    30961 ctagctggcg caaccctgtg ggcgaatcag aattgtcatt acctgtaatc atgacaatgt
    31021 tttgacaacc gtgtttttgc aaagtttgtg cggctaattt accaccttca aaattatctg
    31081 aagaaataac cggaatattt ggcgccagat tacggtcaaa agctacaata ggagcttcga
    31141 cacgctcata atcctcaatt cccaaattat gactggaaga aataatgccg tccacttgat
    31201 tggccgcaag catttctaag tattcgcgtt ctttgacggg attatgttca ctattgcaaa
    31261 taatagtttt gtagccctgt ttaaacaatt caatttccaa atgttcaatt aattcagcgt
    31321 aaaagatatt gctaatgttg gggaagataa gcccaatgag ttgggttgat ttaccttgca
    31381 aacttctagc gagattgtta ggcttgtagc ccaattcacg catggcctta ttcaccttat
    31441 tcactgtttt ttgggaaagg tagccttttt ggttgattac tcgcgaaaca gttgttggac
    31501 tgacacccgc caaggctgcc acgtctgtta attttgcgac cacttgccta atacctcatt
    31561 tcataataat gtccaaaagc ttgtccttct actacttgga tacctgtttg tccttgggtt
    31621 gggaaaacac gactagttag gactttttgc ccttggttaa taaagatttc aagaatggaa
    31681 ttgtccacat agacattaag tgtaacatgg ccttgtggga tttggcaaga gcgcaccgta
    31741 ccatagtctt gggcatattg aacccctgcc cgacttcggt caatgcttaa ctgtcctttt
    31801 gttgtatcca ccttcaatct taggccattc cctttttggt cggcaaatag gagcaggtca
    31861 agcttttttt ggcctggaat cgtcaattcc aattcataac agttagaact gtctatttta
    31921 ttatgaaata attctgctgg ccctcgaaga ttttgtaggg ctgaaacagg tgtttgatag
    31981 agaattccgt ctttaatcct taattctttc actaaactaa gagctccttg atagtcataa
    32041 cgatctgtcg gataatcaat atctggtaac ccaatccaag acacggctag aaccctgcca
    32101 tctggactac taaaggcttg agtggcataa gcttcaaaac caaagtcaag attttgaagg
    32161 gcccccccac ccagcaattg gccagtttct ggattgaagc tttcaaaaat tttataggta
    32221 ttggggtaga tattttgata atctaaatca gctttagcca gtccttgtgg gctgaaaatc
    32281 aaaacaggct tgtcatcgac aaacacaagg ttggggcatt caatcatgta ttccgtgcca
    32341 gaatcgtcaa agtccaaatc ggctataaat cgccagttgt ccacatgatt gtccacagct
    32401 ttgtaaagtt tgatttttcc tttgccatct aaaccttggg cgccaataat agcatagaat
    32461 tgaccttgat aagaaaaaag ctgaggatcc cgaaaatgtt ctgtaacatc atcgggctgt
    32521 tcaatcaaaa cctgtggtat tttggaaata tttccttgtt tatccatcca tgcgccaacc
    32581 tgtaatggtg ttctgaccca attggcatcg cgaacattac cagtgtaaaa gaggaagagc
    32641 ttgtcatcaa tagcataggc agaacctgag taagccccat gactatcgtg agcgtggtct
    32701 ggcagaaggc ggctccttgt ttccgtaaaa tggacgaggt ctgtggatgt catgtgtacc
    32761 cattgtttca gaccatgcgc agccccataa ggccagtttt gataaaaaag atggtagtga
    32821 ccgttgaaat aagaaaaccc attggggtca ttgagtaatc ctgtcttagg ttcgacatga
    32881 aattggctgt gccatgggga ttgggccatt ttttcagtaa tagcttggta gtcttttgag
    32941 ctccactctt tgtaagggcg gtagcgaata gcttgtggta gatccatcaa aatctccttg
    33001 ttttatagtc gtcttcaaga atatactaaa cgttttctgt ttaaaaatca agaatttaaa
    33061 gcatataaaa taaaaatatg ttaaacgctt gacataaaaa atattagtga taaaatgata
    33121 gtcaagaagt gaagcggatt cactagcgtt ggtcatgcac attcttagcc acaaagctgg
    33181 cagaatggat gagggactaa caccaaatga aaaaggagac ttttaggcat ggataatcgt
    33241 cagattgcaa ctgaagtgat tgaggcttta ggtggccgag aaaatgtgag aagtgttgcc
    33301 cactgtgcaa ctcgccttcg cgtgatggtt catgatgaag gaaagattga taaggaaaaa
    33361 gcagaagcta ttgacaaggt taaaggagct ttctttaatt ctggtcaata tcaaatgatt
    33421 tttggaactg gtaccgtcaa taacatttat gacgaagtcg ttgctcttgg tttaccaaca
    33481 tcatcaacca gtgagcaaaa ggcagaagca ggcaaacaag gcaatatctt ccaacgggcg
    33541 attcgtacgt ttggagatgt ttttgttccc attattccag ctattgtagc aacgggtctc
    33601 tttatggggg ttcgtggttt ggtgacccag ccagctatta tggatttatt tggggtgcat
    33661 gagtacgggg aaaattttct catgtacact cgtattctaa cggatacagc ctttgtctac
    33721 ttgccagctt tggtggcttg gtcagccttt agggtctttg ggggtaatcc tattatcggt
    33781 attgttttag gtttgatgct ggtgtccaat gaattgccta atgcttgggt agttgcctct
    33841 ggtggagatg ttaagccgct aaccttcttt ggatttgttc ctgttgttgg ttatcaaggt
    33901 accgttttac ctgccttctt tgttggtctg gtaggagcta agttggagaa atggttgcac
    33961 aaaaaggttc cagaagcttt agacttattg gtcacaccgt ttttaacgtt tgctattatg
    34021 agtaccttgg gactatttgt gattggacca gttttccatt ctcttgaaaa ccttgttctg
    34081 gctgggacac aggccgtctt gcatttgccg tttggcattg ctggtcttat tgttggtggt
    34141 atccaacagt tgatcgtcgt tactggtatt caccatatct ttaacttcct agaagcgcag
    34201 ctaattgcca ataccggaaa agatcctttc aatgcttacc taacagcagc aacagctgct
    34261 caagctggag ctaccttagc tgttgctgtt aaaaccaaat caacaaaact aaaaggtctg
    34321 gcctttccat caaccttgtc agctctttta gggattactg aaccagctat ttttggggtc
    34381 aacctccgtt atccaaaagt ctttgtttca ggtcttattg gtggtgcctt aggtggttgg
    34441 gtcgctgggc tctttggcat tgcaggaact ggttttggga ttacggtctt accaggaact
    34501 ctcctttact tgaatggcca tttattacaa taccttgtga ctatgcttgt cggtcttggg
    34561 gtagcctttg gaattgctta tacttggggt tatcaagata gagaaaccct tccgttacca
    34621 gctgtcgaag ttgatcaaac ggctgatcag ccagccttag cagaggaaac tttatatagt
    34681 cctttgaatg gaacagttgt agatttagca gctgtttcag accctgtttt ttcatcaggt
    34741 gctatgggtc aaggcttagc aattaagcca gaagataata ccctatactc accagttgat
    34801 ggtaaagttg aaattgtctt tgaaacaggt catgcctatg ctataacgtc aagtcaagga
    34861 gcagaagtgt tacttcatat tggtattgac actgtgtcga tggcaggaga tggctttgaa
    34921 tctcttgtag cagtaggtca ggcggttaaa aaaggtgatc ttcttggaca ctttgatcct
    34981 agcaagattg cagaagctgg actagatgac accaccatga tgattgtgac caatagcgct
    35041 gactatcaaa gcgtggacat tcttgctcaa ggacatgttt tgattggtga tcaagtggca
    35101 cttatcaaat aaggacaaaa gagctaacta agcagctctt ttgtcctgcc attcatggca
    35161 ggttggcaaa aaaatgagaa aagcctaaaa accttaaatt tgtgttattt tacctatact
    35221 ttgtaccttg ttttttttaa taaagtgttg ttatacttaa ggcgaactat aggaatgcgc
    35281 ttacatagat ggtatatcaa ctgggaagcc atgacttagt accaaaaata aggagtgtcc
    35341 aaatggataa acatttgttg gtaaaaagaa cactagggtg tgtttgtgct gcaacgttga
    35401 tgggagctgc cttagcgacc caccatgatt cactcaatac tgtaaaagcg gaggagaaga
    35461 ctgttcaggt tcagaaagaa ttatcttcta tagatagctt gcattatctg tcagagaata
    35521 gcaaaaaaga atttaaagaa gaactctcaa aagcggggca agaatctcaa aaggtcaaag
    35581 agatattagc aaaagctcag caggcagata aacaagctca agaacttgcc aaaatgaaaa
    35641 ttcctgagaa aataccgatg aaaccgttac atggtcctct ctacggtggt tactttagaa
    35701 cttggcatga caaaacatca gatccaacag aaaaagacaa agttaactcg atgggagagc
    35761 ttcctaaaga agtagatcta gcctttattt tccacgattg gacaaaagat tatagccttt
    35821 tttggaaaga attggccacc aaacatgtgc caaagttaaa caagcaaggg acacgtgtca
    35881 ttcgtaccat tccatggcgt ttcctagctg gaggtgataa cagtggtatt gcagaagata
    35941 ccagtaaata cccaaataca ccagagggaa ataaagcttt agccaaagct attgttgatg
    36001 aatatgttta taaatacaac cttgatggct tagatgtgga tgttgaacat gatagtatcc
    36061 cgaaagtaaa tggagaagct agtgacgaaa atctcaagcg atctattgat gtttttgaag
    36121 agattgggaa attaattgga ccaaaaggtg ctgataaatc gcgtctgttt attatggata
    36181 gcacctacat ggctgataaa aacccattga ttgagcgagg agctccttat attgatttgt
    36241 tattggtaca agtctatggc gcaagaggag aacaaggtga gtttcaaaac gataccaagt
    36301 tagttacaga tacccctgaa gaacgatggc aaggttatag caagtatatt cgtcctgaac
    36361 aatacatgat tggtttttct ttctatgagg aaagagcagg cagcggcaat ctttggtatg
    36421 atattaattc tcgcaaggac gaggacaaag caaatggaat taacactgac ataactggaa
    36481 cgcgtgccga acggtatgca aggtggcaac ctaaaacagg tggggttaag ggaggaatct
    36541 tctcctatgc tgtggatcgt gatggtgtag ctcatcaacc caaacaaata gctgaaaaag
    36601 ataaacagaa tgtaaaaaat aatcaacctc agattccaga gattactgat aacattttcc
    36661 actcagatta tagtgtctcc aaggcactaa agacagttat gctaaaagat aagtcgtatg
    36721 atctgattga tgagaaagat ttcccagata aggctttgcg agaagctgtg atggcgcagg
    36781 ttggaaccag aaaaggtgat ttggaacgtt tcaatggcat attacgattg gataatccag
    36841 cgattcaaag tttagaaggt ctaaataaat ttaaaaaatt agctcaatta gacttgattg
    36901 gcttatctcg cattacaaag ctcgaccaat ctgttttacc cgctaatatg aagccaggca
    36961 aagatacctt ggaaacagtt cttgaaacct ataaaaagga taacaaagaa gaacctgcta
    37021 ctatcccacc agtatctttg aaggtttctg gtttaactgg tctgaaagaa ttagatttgt
    37081 caggttttga ccgtgaaacc ttggctggtc ttgatgtcgc tactctaacg tctttagaaa
    37141 aagttgatat ttctggcaac aaacttgatt tggctccagg aacagaaaat cgacaaattt
    37201 ttgatactat gctatcaact gtcagcaatc atgttggaag caatgaacaa acagtgaaat
    37261 ttgacaagca aaaaccaact gggcattacc cagataccta tggcaaaact agtctgcgct
    37321 taccagtggc aaatggaaaa gttgatttgc aaagccagct tttgtttggg actgtgacga
    37381 atcaaggaac cctaatcaat agcgaagcag actataaggc ttaccaaaat cagcagattg
    37441 ctggacgtag ctttgttgat tcaaactatc attacaataa ctttaaagtt tcttatgaga
    37501 actataccgt taaagtaact gattccacat tgggaaccac tactgacaaa acgctagcga
    37561 ctgataaaga agagacctat aaggttgact tctttagccc agcagataag acaaaagctg
    37621 ttcatactgc taaagtgatt gttggtgacg aaaaaaccat gatggttaat ttggcagaag
    37681 gcgcaacagt tattggagga agcgctgatc ctgtaaatgc aagaaaggta tttgatgggc
    37741 aactgggcag tgagactgat aatatctctt taggatggga ttctaagcaa agtattatat
    37801 ttaaattgaa agaagatgga ttaataaagt attggcgttt cttcaatgat tcagcccgaa
    37861 atcctaagac aaccaataaa cctattcagg aagcaagtct gcaaattttt aatatcaaag
    37921 attataatct agataatttg ttggaaaatc ccaataaatt tgatgatgaa aaatattgga
    37981 ttactgtaga tacttacagt gcacaaggag agagagctac tgcattcagt aatacattaa
    38041 ataatattac tagtaaatat tggcgagttg tctttgatac taaaggagat agatatagtt
    38101 cgccagtagt ccctgaactc caaattttag gttatccgtt acctaacgcc gacactatca
    38161 tgaaaacagt aactattgct aaaggtctat ctcaacaaaa agataagttt tctcaaaaga
    38221 tgcttgatga gttaaaaata aaagagatgg ctttagaaac ttctttgaac agtaagattt
    38281 ttgatgtaac tgctattaat gctaatgctg gagttttgaa agattgtatt gaaaaaaggc
    38341 agctgctaaa aaaataaaca aagcaacttt cttagatagc aacattcaga ttaaattaac
    38401 aaaatgtgac tatgataaag gtttgctgga attgattaac caaaagacta aaaatctgag
    38461 atgaatagtc ccagattttt agtcttttat aggttttgat gacataaagc taaataatcg
    38521 ttagactacc agaaagtggc acttgtccgt gagacatggc tgtcttgata agagcctgtg
    38581 tcatcataaa gataaaaaag aacaagaaaa aggagaaaac atgggaaaat tatatggaag
    38641 cattgaagct ggtgggacaa agtttgtctg tgccgtgggt gatgaggagt ttacagtggt
    38701 ggataaaaca caatttctga ccaccacacc agaagaaacc atcgcgcgaa ccatcgctta
    38761 ttttaaagct tttgaggcag atttggctgg aatggcgatt ggttcttttg gaccaattga
    38821 tattgatccg tcgtcagaga cttacggtca tatcacaacc actcctaagt caggctgggc
    38881 taatgtggat ttgttaggac aactttcggc tgcttttaag attccttttg atgtgacaac
    38941 agatgttaat agctctgctt atggtgaggt gcttgctcgt cctggtgtgg aaagtttggt
    39001 ctattacacc ataggtactg gtattggggc gggagctatt caacatggcc actttattgg
    39061 gggattaggt catacagaag cgggtcatac ctatgtcatg ccccatcctg atgatatggc
    39121 caagggattt ttaggtgttt gcccttttca taaagggtgt ctggaaggga tggcagcagg
    39181 tccaagtatt gaggccagaa cgggagtgag aggtgaacgg cttgatcagg aagctgatgt
    39241 ttgggacatt caagccttct acattgccca agcagcgctg caagcaacta tgctttaccg
    39301 tcctcaagtg attgtttttg gcggaggtgt catgacgcaa gaacatatgg tgttgcgcgt
    39361 gcatgataaa ttcactaccc ttttaagcgg ttatttgcca gtccctgatt tgacagacta
    39421 cattgttact ccagctgttg ctgataatgg ctccgctact ctgggaaatt ttgctttggc
    39481 caagttggct gcacaaggaa aatagggtct gtttttgcct gcttgtctag taaaagaaag
    39541 cgcagtcagc tagaacatgc tataataaac agagattaac atcagaagcg aggaatatta
    39601 tgtcagaacc attatttcta aaatcaacca tgcacgacag aatttggggt ggcaccaagt
    39661 tgagagatgt ctttgcttac aacatcccaa gtgacaccac aggtgagtat tgggctattt
    39721 ctgctcatcc taatggtgtt tcgacagtta ctaatggacg ttatcaagga caacctttaa
    39781 atacattata tgcgcaagag ccggccttgt ttgggaatcc aaaggaagaa gtttttccac
    39841 tgttaactaa gattttagat gctaatgact ggctaagtgt ccaagttcat cctgatgatg
    39901 cttatggacg tgaacatgaa ggagaattag gcaagacaga atgctggtat attatttctg
    39961 ccgaagaagg ttctgaaatt gtatacggcc atcaggccaa gtccaaagaa gaccttcgtg
    40021 ccatgattga agcaggcgct tgggatgatt tgctgactcg tgtcccagtc aaagcaggcg
    40081 attttttcta tgttccaagt ggcaccatgc atgcgatcgg caagggaatt cttattttag
    40141 aaacccagca gtcatctgat accacttatc gggtttatga ttttgaccgt aaggacgtta
    40201 atggtaatct tcgtgacctt catattgaaa agtctattga tgtcttaacg attggtaagc
    40261 cagaaaatag cgtgccagcc actatagttt tggataatat ggtagaaaca actttagtct
    40321 caacgccatt tttcactgtt tacaaatggg taactagtca aatggttgac atgaaacaag
    40381 ctgcgcctta tcttttagtg agtgttttaa aaggtcaagg aaaactctat gtggaccaga
    40441 aggcttatga gctagaaaaa ggcatgcact ttatcttgcc taacgatgtt aaatcgtggt
    40501 catttgatgg tcaattagaa atgattgtga gtcaccccaa tcaactcgtt taataccatg
    40561 gtatcaatgt gagaaaaaag gataaaatta tgactctctg tttctctgat aactttacca
    40621 acctttcttg aactgcctac atatttatgt taaaatgaaa tgattagatg atcgaaagga
    40681 aagagaatgg ccaatattct acgcaaagtt atcgaaaatg acaaaggcga actaagaaaa
    40741 ttagaaaaaa ttgcaaaaaa agtagaatcc tacgcagacc aaatggtgtc attgtcggat
    40801 agagatctac aaggaaaaac acttgaattt aaagaacgct atcaaaaagg tgagaccctc
    40861 gaacaactat tacctgaggc tttcgcagtt gttcgtgaag cagcaaaacg tgtgttgggc
    40921 ttgttccctt atcgtgttca aattatggga ggaattgttc ttcataacgg ggatgtccct
    40981 gaaatgcgta ctggtgaagg aaaaactctt acagccacca tgccagttta tctcaatgct
    41041 attgctggtg agggtgttca cgttattact gttaatgagt atctttcaac gcgtgacgct
    41101 accgaaatgg gtgaagtgta tagctggtta ggtctttcag ttgggatcaa cctagctgct
    41161 aagtctcctg ctgaaaaacg tgaagcttat aattgtgata ttacttattc aaccaactct
    41221 gaagtcggtt ttgattacct tcgtgacaac atggttgttc gccaggaaga catggtacaa
    41281 cgcccactca attttgcctt ggttgatgaa gtggactcag tcttaattga cgaggcaagg
    41341 acacctttaa ttgtctctgg tgctgttagc tctgaaacca atcaattgta tattcgtgct
    41401 gacatgtttg taaaaactct gacgtcagtg gattatgtta ttgatgtgcc cactaaaacg
    41461 attggattaa gcgattcagg tattgataaa gctgagagtt attttaactt gagcaatctt
    41521 tacgatattg aaaatgtagc cttgactcac tttatagaca acgcccttcg tgcaaactat
    41581 attatgttgt tagatatcga ttatgtggtc agcgaggatg gtgagattct tattgttgac
    41641 caatttacag gtcgtacgat ggaagggcgt cgcttctcag acggcttgca ccaagctatt
    41701 gaggcaaaag aaggcgttcg tattcaggaa gaatcaaaaa ccagcgcctc aatcacctac
    41761 caaaatatgt ttcgtatgta caaaaaattg gcaggtatga ctggtactgc gaagaccgag
    41821 gaagaagaat ttcgcgaagt ttataacatg cgtattattc caattccaac caatcgtcca
    41881 attgcccgta ttgatcacac tgatttgctt tacccgacct tagaatcaaa gtttagagca
    41941 gttgtggaag acgttaaaac gcgtcatgca aaaggacaac ctatccttgt aggtacggtt
    42001 gctgttgaaa ctagtgattt gatttcacgc aagctggttg aagcagggat tcctcatgag
    42061 gttttgaatg ctaaaaatca ctttaaagaa gctcaaatca ttatgaatgc tgggcaacgt
    42121 ggtgctgtta caatcgcaac taacatggct ggtcgtggta ctgatatcaa gcttggtgaa
    42181 ggtgttcgtg aacttggtgg gttatgtgtc attggtacag agcgtcatga aagccgtcgt
    42241 atcgataacc agcttcgtgg acgttcaggt cgtcaaggag atccaggtga atcccaattc
    42301 tatctttcat tagaagatga tttgatgaga cgtttcggct ctgatcgtat aaaagccttt
    42361 ttggatcgga tgaaattaga tgaagaagac actgttatta aatcaggtat gttagggcgt
    42421 caagtggaat ctgctcaaaa acgggttgaa ggaaataact acgatacccg taaacaggtc
    42481 ttacaatatg atgatgtgat gcgtgaacag cgtgaaatta tctatgctaa ccgtcgtgat
    42541 gtcataactg ctaatcgaga tcttggccca gaaattaaag ctatgatcaa gcgtactatc
    42601 gaccgtgctg tagatgctca tgcacgtagc aatcgtaaag atgctataga tgctattgta
    42661 acttttgcta ggacctcgct ggtaccagaa gagtctatta gtgctaaaga gttacgtggt
    42721 ttaaaagatg atcaaatcaa agagaagctt tatcaaagag cgcttgctat ctacgaccag
    42781 caactatcaa aacttcgtga tcaagaagct attattgaat tccaaaaagt actcattttg
    42841 atgattgtgg ataacaaatg gactgaacac attgatgcct tagatcaact tcgtaatgct
    42901 gttggacttc gggggtatgc tcaaaataac cctgtggtgg aataccaagc agaaggtttc
    42961 aagatgttcc aagatatgat tggtgctatc gagtttgatg taacacgtac catgatgaaa
    43021 gcacaaattc acgaacaaga acgtgaacgt gcaagtcaac gtgccacaac agcagcacct
    43081 caaaacatcc aatcgcagca gtcagctaac accgatgatt tacccaaagt ggagcgtaac
    43141 gaagcttgtc catgtggttc aggcaaaaaa ttcaaaaatt gtcacggtcg taaatccttc
    43201 tcataagaac gagacacaac aggacaaagt taggtatata gaaagaagct gagaataatt
    43261 ctcagcttct cactgtttaa atcttctaac attaagatga aataaggttt ggtggaaagt
    43321 aatgattgtt ggacatggaa tagacttaca agagatttca gctattgaaa aagtttatca
    43381 aagaaatcct cgctttgctc aaaaaatttt aactgagcag gaattggcta tttttgagag
    43441 ttttccttat aaacgtcgac tcagctattt ggcgggtcgt tgggcaggca aagaggcttt
    43501 tgctaaggct atagggacag gtattgggcg gttaaccttt caagatattg agattttaaa
    43561 tgatgttaga ggatgtccca ttctgacaaa atctccgttt aaaggaaata gttttattag
    43621 catctcacat agtggcaatt atgtacaggc tagtgttatt ttggaggata aaaaatgatt
    43681 tcaagtttcc atcgcccaac agttgcaagg gtcaacttac aagctattaa ggagaatgtt
    43741 gccagtgttc aaaagcatat tccactaggg gtaaaaacgt atgcagttgt caaggctgat
    43801 gcttatggtc atggtgctgt ccaggtgtca aaagcactcc tacctcaagt ggatgggtac
    43861 tgtgtgtcaa atcttgatga ggctttgcaa ttacgtcaag caggcattga taaagagatt
    43921 ttaattcttg gagtcctcct gccaaatgaa ttagagttag cagttgctaa tgctattact
    43981 gttacaatcg ctagtttaga ctggatagct ttagctagac tggagaaaaa agaatgtcaa
    44041 ggcttaaaag ttcatgtaaa agttgattct ggtatggggc ggatcgggct tcgttcttca
    44101 aaagcagtca atttattgat tgatagtcta aaagagttgg gtgctgatgt agaaggtatt
    44161 ttcactcatt ttgccacagc tgatgaggca gatgatacta aatttaacca gcagttacag
    44221 ttttttaaaa agctgatagc tggacttgag gataagcctc gtttagtaca tgctagtaat
    44281 tcagccacaa gtatctggca tagtgatacc atttttaatg ctgttcgttt aggaattgtt
    44341 agttatggtt tgaatccaag tggttctgat ctaagcttac catttccact gcaagaggct
    44401 ttatctctag aatctagctt agtgcatgtc aagatgattt cagctggtga tacagtcggt
    44461 tatggagcta cttatactgc caaaaagtct gaatatgtag ggaccgtccc aatcggttat
    44521 gcagatggct ggaccaggaa catgcaaggc ttttcggtgt tagttgatgg acaattctgt
    44581 gaaattatag ggcgtgtatc gatggatcaa ctgaccatac gacttcccaa agcatatcct
    44641 ttaggaacaa aagtcacttt gattggcagc aatcagcaaa aaaatatttc tacaacagat
    44701 atcgcaaatt accgtaatac aatcaattat gaagttctat gccttttaag tgaccgtatt
    44761 cctcggatat attaagaaaa tcacgtaaaa ataattgcat tgtgcttatc tttaatgtta
    44821 taataaagtg aggacattag aaagaagtga ctaattaata tatgaataaa aacaaactat
    44881 taagagttgc catgctacta agtctcttag ccccgacagc agaaagcatg acagtgttgg
    44941 ctcaagatgt aatgcttgag acgcataaag caactacaaa tgaaaccagt gattcttctt
    45001 caaaagagga aaataataaa aatgcagcac ctacaacatc agataaaact gaccaaggtc
    45061 cccttgatgc ttctgcagaa acaaactcta atagtcttgt taacgcggat gataaaaaaa
    45121 gaagcgattc tagtcagtct gctgtaggct cttcgaacaa caaggcagaa gcagaaaacc
    45181 aggtagatga taaatcaact gatcattcga aaccaactga ccagccaaaa ccgactgatc
    45241 agcccaaacc atcaccatct aaagttgata cggcacctgc ttcttcattg tcgagacagc
    45301 tgccagaggc aagaactcct attcagtcgt tgtcccctta cgtatcagat ttagatttga
    45361 gtgagataga tatcccttct gtcaacacat acgcggcata tgtagagcat tggagtggta
    45421 aaaatgccta tacccatcat cttttatctc gccgttatgg tattaaagct gaccagattg
    45481 atagttactt aaaatcaaca ggcattgcct atgacagcac acgtattaat ggtgagaagc
    45541 tattgcaatg ggaaaagaaa agtgggctgg atgttcgagc tatcgtagct attgcgatgt
    45601 ctgagagttc tttaggaact caagggattg cgactttgct tggagctaat atgtttggct
    45661 atgcagcttt tgatctagat ccgactcaag caagtaagtt taatgatgat agtgctattg
    45721 tcaaaatgac acaagatacc attattaaaa acaaaaatag caattttgca cttcaagatt
    45781 taaaagcggc taagttttca cgaggtcaat taaactttgc aagtgacggg ggtgtttatt
    45841 ttactgatac tactggtagt ggtaaacgtc gcgcacaaat tatggaagac ctggataagt
    45901 ggattgatga ccatggtggc acaccagcca ttccagccga attgaaagtg cagtcatcag
    45961 ctagttttgc atctgtgcca gcaggttata agctctctaa gagttatgat gtcttgggtt
    46021 atcaagcttc gagttatgct tggggacaat gcacttggta tgtgtataat cgcgccaaag
    46081 aattgggtta ccaatttgat ccttttatgg gaaatggtgg agattggaag tataaagcag
    46141 ggtatgccct gtcaaagact ccaaaagtag gttatgctat ttcatttgca ccagggcaag
    46201 caggcgctga tggcacttat ggccacgtat caattgtaga agatgttaga aaagatgggt
    46261 ctattcttat ttcagagtct aactgtatcg gcttaggtaa gatttcttat cgtaccttta
    46321 cagctcagca ggctgaacag ctaacatatg ttattggcaa gagtaaaaac taataactaa
    46381 tgacttttct taaggcttaa ttttcttagc gggagttagg ccttttaaac tgttggtgta
    46441 acacgataaa gtttcaagta aataaatgat tttgagacaa tagcacgatt agggctattg
    46501 tcttttttta ttgtgatgaa tatgcagtag tgacatctca tgttgtctcc taacacaatt
    46561 atggagctta taacgtgtta catgttttgt gaatgaagta atgattgaat cagccctagt
    46621 aataactatc ctaaggctag ctttatctca gtaatattgt tgtagaggaa ttcttttatc
    46681 agagatatca tcattatcaa tccaggctta ttgctagtgt cttggctcac atagtggagc
    46741 tttttaacag acaattggac cattggaaac aatcttgaca aaataatcac aaagtgatag
    46801 attaagttca gttaataaaa aagttagctt attcataaaa atttttagaa agttgattga
    46861 aaatgaaaaa aatcagcaaa tgtgcgtttg tggcaatatc tgcccttgtt ctcattcagg
    46921 ctactcaaac tgtaaaatca caagagcctt tagttcagtc acaactcgtg acaacagtag
    46981 ctttaactca ggataatcga cttttagttg aagagatagg cccttacgct agtcaatcag
    47041 ctggaaaaga gtattataaa catattgaaa aggttattgt tgataatgat gtctatgaaa
    47101 aaagcctgga aggcgagcga acctttgata ttaactacca agggattaag atcaatgcta
    47161 accttattaa agacggtaag catgaattga ctattgttaa taaaaaagat ggtgatatcc
    47221 taattacctt tattaaaaag ggcgataaag tgacctttat ttcagctcaa aaattaggaa
    47281 caacagatca tcaggattca ttaaaaaaag atgtgctcag tgataaaaca gtgccacaaa
    47341 accaaggcac acaaaaagtt gttaaatctg ggaaaaatac tgctaacttg tcattaataa
    47401 caaaattgag tcaagaagat ggtgcaattt tatttccaga aattgatcgt tattctgata
    47461 acaaacagat aaaagcattg actcagcaaa tcacaaaggt tacagtcaat ggtacagttt
    47521 ataaagatct tatttcagat tctgtaaaag atactaatgg ctgggtctcg aatatgacag
    47581 ggcttcatct tggaacaaaa gctttcaaag atggagaaaa tacaatcgtg atatcctcaa
    47641 aaggatttga agacgttacc attaccgtta ccaagaaaga tggtcaaatc cattttgtat
    47701 ctgccaaaca aaaacaacat gtgactgctg aagacagaca atcaacaaag ttggatgtca
    47761 ccactttgaa aaaagctatc aaagaagcgg atgcgattat tgctaaagaa agcaacaaag
    47821 acgcggtcaa agatctggct gagaaacttc aagtcatcaa ggattcttac aaagaaatca
    47881 aagatagtaa gctactcgcc gatactcatc gactgttaaa agataccatc gagtcttatc
    47941 aagcaggtga ggtttctatt aacaatctca cagaaggaac ctatacgcta aactttaaag
    48001 ctaataaaga aaactcagaa gagtcctcca tgcttcaagg tgcttttgat aaaagagcca
    48061 aattagtggt taaagcagat ggtacaatgg aaatttccat gcttaatact gctttaggac
    48121 aatttttgat tgacttttct attgaaagca aagggaccta cccagcagca gtgcgtaaac
    48181 aagttggcca aaaagatatc aatggtagct atattcgaag cgaatttacc atgcctattg
    48241 atgatttgga taaattacac aaaggtgctg ttttggtatc agccatggga ggtcaagaaa
    48301 gtgatttaaa ccactatgac aaatacacca aacttgacat gacctttagt aagaccgtta
    48361 ccaaaggctg gagtggttat caggtagaaa ctgatgataa agaaaaaggg gttgggactg
    48421 aacgtcttga aaaagtttta gttaaacttg gcaaagattt agacggcgat ggtaaattat
    48481 caaaaacgga attagaacag attcgaggcg agttgcgtct agaccattac gagttaactg
    48541 atatttcttt attgaaacat gctaaaaata ttacagaact acatctggat ggaaaccaaa
    48601 ttacggaaat tccaaaagag ttatttagtc aaatgaagca acttcgattt cttaacttaa
    48661 gaagtaatca tttaacttat ctagacaaag atacatttaa aagcaacgct caattaagag
    48721 aactctactt atcaagtaac tttattcact ctcttgaagg aggactattc cagtcgcttc
    48781 atcacctgga gcaacttgat ctttccaaga atcgtattgg ccgactttgt gataacccat
    48841 ttgaaggatt gtctcgcctg acttcattag gtttcgcaga aaatagtctt gaggagatac
    48901 ctgaaaaagc gctagagcct ctaacatcac ttaactttat cgacttatct caaaataatt
    48961 tagcactact gccaaaaaca atagaaaaat tgcgcgcctt aagcactatt gtggcaagta
    49021 gaaatcatat tactcgtatt gataatattt catttaaaaa tcttcctaaa ttatctgtac
    49081 tcgatttatc aactaatgaa atttcaaatc ttccaaatgg tatatttaaa cagaataacc
    49141 aattaacaaa acttgatttt ttcaataact tgcttactca ggttgaagaa tcagtatttc
    49201 cagatgttga aacgcttaat ttagatgtga agttcaatca gataaaaagt gtgagtccaa
    49261 aagtaagagc tcttatcgga caacacaaac tgactccaca aaaacatatt gcaaaacttg
    49321 aagcttcctt agatggcgaa aaaataaaat atcatcaagc tttcagtctt ttagatttgt
    49381 attattggga gcaaaaaaca aattctgcca ttgataaaga actagtgtct attgaagaat
    49441 atcaacaatt gttacaagaa aaaggttcag atactgtctc tttacttaat gatatgcaag
    49501 tcgattggag tattgtgatt cagttgcaaa aaaaagcttc caacggacag tatgtgacgg
    49561 ttgacgaaaa gcttctctca aatgatccga aagatgactt aacgggagag ttttctttaa
    49621 aagatccagg tacatatcgg attcgcaaag ctttaataac aaagaaattt gctactcaaa
    49681 aagaacatat ctatttgaca tctaatgata tccttgtggc gaaaggacca cattcacatc
    49741 aaaaagattt agttgagaaa ggccttagag cattaaatca aaaacaattg cgtgatggta
    49801 tttactattt aaatgccagc atgttaaaaa ctgacttagc atctgagtcc atgtcaaaca
    49861 aggctattaa tcatcgagtg actttggtag ttaaaaaagg tgtttcctat ttagaagttg
    49921 agtttagagg gataaaggtt ggtaaaatgt taggctacct tggtgaattg agctatttcg
    49981 tagatggtta ccaaagagat ttagctggta aaccagttgg tcgaacaaaa aaggcagagg
    50041 ttgtgtctta tttcacggat gtaactggcc taccattggc agatcgttat ggaaaaaact
    50101 atccaaaagt gctgcgtatg aaattgattg aacaagcgaa gaaagacgga cttgtgccat
    50161 tacaggtctt tgtgcctatc atggatgcca tttcaaaagg gtctggcctt caaaccgttt
    50221 ttatgcgttt agactgggca agccttacga cagagaaggc aaaggttgtc aaagaaacta
    50281 ataatccaca agaaaatagc catctaactt caacagatca gttgaaagga cctcaaaatc
    50341 gtcaacaaga aaaaacacct acaagtcctc cttcagcagc tactggtatt gctaacttaa
    50401 ctgatctcct ggctaaaaaa gcaaccggcc aatcaactca agaaacttct aagacagatg
    50461 atactgataa ggcagagaaa ttgaaacagt tagtgcgtga ccatcaaaca tcaattgaag
    50521 gtaaaacggc aaaagatact aagactaaaa aatctgataa gaaacatcgt tccaatcaac
    50581 aatcaaatgg tgaagaaagt agctctcgtt atcacttaat tgcaggtcta tctagcttta
    50641 tgatcgtagc tctgggattc attattggtc gaaagacatt atttaaataa gaaacttagt
    50701 accaagtgaa ataacaaaaa agctagtcag caagagctaa aaacatacct atttccgaca
    50761 ggactcctga tacaggttga agtagtcgca agataggtgt gtttttcaca tttggagatg
    50821 ttataaaatg ggaatcattt aaaaaatgaa ataagatgtt taagaaaaga tagacaagag
    50881 gagcatatga caaaggtagt aataaagcaa ttgttgcagg taatagtagt gttcatgata
    50941 agtttatcga caatgacagg tgttgtgtat gcagataaag gtcaaattta tggatgtatt
    51001 attcagagaa attatcgtca tccgatatct gggcagatag aagatagcgg aggtgagcat
    51061 tcctttgata ttggtcaagg tatggttgaa gggactgtct atagtgatgc catgctagaa
    51121 gtatctgatg ctggaaagat cgttcttact tttcgtatga gcttagctga ttattcgggc
    51181 aattatcagt tctggataca acctggagga actggaagct ttcaggcagt tgattacaat
    51241 atcacccaaa aaggtactga taccaatggg acaacactag atattgctat tagtttgcct
    51301 actgtaaata gtatcattcg aggaagcatg tttgttgaac ctatgggcag agaagtggtc
    51361 ttttacctgt cagcttcaga gttaatacaa aagtattcag gtaatatgtt ggcccagctt
    51421 gttactgaaa cagacaattc tcaaaatcaa gaagtaaaag acaatcaaaa gcctgttgat
    51481 acaaaactag gagaaagtca agatgagtct cacacaggtg ctatgataac tcagaataag
    51541 cctaaagcca actcatcaaa taacaagagt ctatcagata aaaaaattct tccttctaag
    51601 atgggcttaa cgacttcttt agagttaaaa aaagaagata agtttcggtc taaaaaagac
    51661 ttatcaatca tgatttacta ctttccaact ttttttctga tgttaggagg gtttgctgta
    51721 tgggtttgga aaaagaggaa aaaaaatgat aaaacgatgt aaaggaattg gtctagcctt
    51781 aatggccttc tttttggtag cttgtgtgaa tcagcaccat aaaacggcta aagagtctga
    51841 acagcagaga attgtagcca cttcggttgc tgtggttgac atctgtgacc gtttaaattt
    51901 agacctcgtt ggggtttgtg atagtaaatt atataccctt cctaaacgct atgatgctgt
    51961 taagcgtgtg ggtttaccca tgaatcctga tatagagttg attgcttctt tgaaaccaac
    52021 ttggattttg agtcccaatt ctttacaaga agatttggaa cccaagtatc aaaaattgga
    52081 tactgagtat ggttttttga acttacggag tgttgagggc atgtaccagt ccattgatga
    52141 tttagggaac cttttccaac gtcaacaaga agcaaaagaa ttgcgccagc aataccagga
    52201 ctattatcgt gctttccaag ctaaacgtaa ggggaagaaa aagcctaaag tgcttattct
    52261 tatgggcttg ccaggtagtt atttggtggc gacgaaccaa tcttatgtag ggaatctttt
    52321 ggacttggca ggtggtgaga atgtttatca gtcagatgag aaagaatttc tatcagctaa
    52381 tcctgaagac atgctggcta aggagcctga cttgatttta cgaacagctc atgccattcc
    52441 agacaaggta aaagtgatgt ttgacaaaga atttgctgaa aatgatattt ggaaacattt
    52501 tacggcagtc aaggaaggga aagtctatga tttggacaat accctgtttg gcatgagtgc
    52561 taaattgaac tacccagaag ccttggacac cttaacacag ctttttgacc acgtgggaga
    52621 tcattcgtaa catgacagca tcagatttac aaagggatca atccctactg gctaaaaaac
    52681 gtttcagaag tggtctttat ctagttcttt taattctcag tctcctgttt ttggcggtta
    52741 ttgccttatc ccttggtggt ttggcggttt cttatggggc aattgtcaag ggactgtttg
    52801 tggcttatga cccccaagtg gctttgattt atgatttgcg ctttccaaga attgtcattg
    52861 ctttattagc aggagcaggg atagcggttt cgggagttct ttttcaagca gtgttgaaaa
    52921 atcctatttc agaccctgct attataggga tttgtagtgg tgctagtttt atggttttag
    52981 tctctagttt acttctaccg cagttgctgc tgtatggtcc aatcgtatcc tttttaggag
    53041 gcggagtgtc tttcctgctg atttatggtt tagcatggaa gaaggggctt aatcctattc
    53101 ggcttattct aacagggatt gctattaatg ctttatttat ggggttatca actgctttaa
    53161 ccagcttttt cacctcagct agtcccatgg tcaatgctct tttagcaggt catattagtc
    53221 aaaaaacatg ggctgatgta ggggttttat tcccttatac attcattggc ttgttactag
    53281 ctttgttatt gtcaaaaacc tgtaaccttc ttcttttaga tgatcaggtg attcgtcatt
    53341 taggaatcga tgctacagcg ttacggttgg gaatttcctt ggttgctgtt ttattggctt
    53401 cggtagccac ttcgatcgtt ggagtggttt ctttcctagg cttgattgtc cctcatatga
    53461 gccgcttgct ggttggaagt aagcaccaaa tcttaattcc tttttcagct ttgcttggag
    53521 cctttgtctt tttgctagcc gatactttag gaagaagtct tgcttaccca ttggaaatta
    53581 gccctgcgat tataatgagt attgtaggcg ggccttactt tatttacctg ctaaggaggt
    53641 ctgatattat atgagtaaga acagcagtca tattcaggtg caaaaccttt cctttgctta
    53701 tgaggaaaaa cttgttttag atgacctgac ctttagcatt ccaaaaggga aaattaccac
    53761 tattatgggg gcaaatggca gtggcaagtc aaccttatta caattattaa ccaaaaacct
    53821 tccgttaaaa caagggcagg tttggcttga gcaggaagaa ttagcaacga tttctctcaa
    53881 ggcttttgcg aaaaaagtgg cagtggtaca ccaataccat caagtggtcg aaggtttgtt
    53941 agtgggtgaa ttggtagcca tggctaggct tgcccgtcgt tcttttttta aaactactac
    54001 caaagaagat gaggaacgcc ttgcttgggc cttagaagta acggctttga gtgattatgt
    54061 ttatcgtgac ctccaacacc tttctggtgg tgaacagcag agggtttgga ttgccatggc
    54121 ccttgcccag gatacagata ttattttttt agatgagcca acgacttatt tggatattaa
    54181 gtaccagctt gacattctca agctgattac taaaatcaac caagagctag gcctaacgat
    54241 tgtgttagta ttacatgaca tcaatcaggc cttgtctctt tcagaccatc ttattggact
    54301 gaaagatggg cgactttatg cccaagggca accaagagac ctccttgatc aacagtttat
    54361 cagagatgtc tttggtgttg aattgccctt tgttgagaga aatggtcaac agtttgtcct
    54421 cacttcattg gtcaatgaaa ccaatgatat tagaactcaa aggagagata ggaacaagaa
    54481 cagatgatgg aaacagaaga tacaatatca aggggaaaaa ggaaaagact gttaaaacga
    54541 ttacgtgaac gaattgcacc aaaaaggtat ttgctttatg tgtcagcctt tttatcttgg
    54601 ttacaatttg tgatgaggat gatcagtttt tacctcattg ccaaaacgtt ttcgaccttt
    54661 attttgggtc atgctattgc cttaggacgc ttggcaggtt tactcctact tttaaatgtg
    54721 gtaggctttg ttcttgctat attaggcaag caactgcaag ggatcgcttc acaatttgct
    54781 agagattcac tcaaacaatc tttctttgag gcttttattg atttagacgg gcaatttgat
    54841 gctcatgcca gtgatgctga tattttaacg ttggcttcac aggggattga tagtttggat
    54901 acttattatg gttactattt gtctttgtct atgagaacca agtggaactg cacaactatt
    54961 atgatactag tttttttaat ttatccattg gcaggccttg tctttttagg agttctgctt
    55021 ctgattccac tttcaatcgt tgctatgcaa aagcggtcta agcaaattat gagtcattat
    55081 tggtcttctt atatggatgt tggtaattta tttatggatg acttaaaagg tcttaataca
    55141 ctgtattctt atcaagcaat agaacgttac gagcaagaat tttcgggtaa ggcagaacag
    55201 tttcgaaagg ctaccatgtc tttgcttggc tttcagttac aagcagttgg ttatatggat
    55261 gcagttatgt atctgggaat tggtttgtca ggttttttag ccgttcaagc tttggcaaca
    55321 ggccagttat ccttttttaa tttcctcttt tttctcttga ttgctacaga attttttaca
    55381 ccgattcgtg agcagggtta tggcatgcac ttggtcatga tgaataccaa aatggctgac
    55441 cgtatcttta gtttcttgga tagtgttcca gcaaggaaag ataacaagag taaaacagca
    55501 ataaacttta accagattga catccaaaat atttctttag cttatgagaa aaaaacagtg
    55561 cttagtgggg ttaccatgac attaacgaag ggccaactaa cagctatcgc gggggtgtca
    55621 ggacaaggaa aaacaagtct agctcaactc ctcttgaaac gccagtctgc aacgactggg
    55681 catattttat ttgacggttt agacagtgac aatttgttgc aagaaactat caaccagcag
    55741 gttctttatg tatcggacca atctacctta ttgaaccgta gcatttacga taatttaagg
    55801 ttagcagcaa atctctctaa aaaagaaatc ttagattgga tagatcaaca tggtttgtta
    55861 agcttcatca actggctacc agatggtctt gacacaatag ttggtgaaaa tggtaacctg
    55921 ttatcaccag gccaaaagca acaggtgatt tgcgcaagag ctctattaag taagaggtct
    55981 ctttatatct ttgatgaggc aacatctagt ctagatgcag aaaatgaacg tattattgac
    56041 aatttaataa cgagattagc taagacagca atagttattg tgattactca taagatgtct
    56101 cgactgaaag gagctaacca agtactcttt ttaaacacag ggcagcctgc ttgtttaggc
    56161 aaaccttgcg acctttatcg ggaccaacca acttatcgtc acctagttga tactcaagca
    56221 agactggagg caagtattta tggctaaacc taacagatct accttaagcc tggtttggcg
    56281 tttattaacc ctaatgaaaa ctctgctacc ttggattatc ttggctgttg gctttgctgt
    56341 tatgggtttt gtcatcacgg taagtattcc aactggaata gcttatttag gactactagc
    56401 tattagacaa gaagtgattc caatattagc tttatatctt ttgattgctc tagccttttt
    56461 aagaggtttt gttcgttatg gagagcatta ttttggtcat tttgttgctt ttcatagttt
    56521 agcagctttt aggaacttga tctttaagaa actaagagct ctttctccag cttgcttgga
    56581 tagtcaagac agtggttatt tgcttaaaat gattggagaa gatattgagg cattagaagt
    56641 tttttttgcc cacacaatcg cacctatttg tacagcaata ttgtctgcag gtttaatgtt
    56701 ctggtacttt tgtcaaagta gttggcagtt agccttatta gctttggcaa cctatgcttg
    56761 tttagccatt gttataccca tttattttgc taatatttta caggttttac taaaaagcca
    56821 aaatgaaggt cgaaaagatt acctgtctta ttttttagag agtttacgat cagtcaaaga
    56881 tcttttgcaa tttcaggtat tagacgagca atttgaacga ttaataaaaa aaagtaatca
    56941 tgtaaatgct cttgatcgta acgtagcgca agcccaattt ttgcaaatgg cattgacttt
    57001 tttttggctt ggcttaatga tcttagcatt ttcttatatg gtttttgatg ggatatgcca
    57061 tgacagctta tcatttgata aaggcttatt aacttttatt gctttcactg cttcgttttc
    57121 tcctttttta gaactaggac gtttgccatt aggctttaag cgtgctatga atgcggctcg
    57181 aaatattttt gaccttctag acgaaaatgt catagtagac gaaggcacaa aacagataga
    57241 tcgtttaagg agtgtttctt ttgaggacct cagttttgct tatccaaagc gccaagagct
    57301 ggtttttaag gatttatcag ttacctttca agaaaagggg attattggca ttaaaggcga
    57361 atcaggatct ggtaaatcaa ctttggtgaa attaattatg aaatggtata attggaagac
    57421 aggagatatt tttcttaatg ataggaatag ttgcttacta aatgctgcta aattacaaga
    57481 tactattgct tatgtgccac aaacagcgca gcttttccaa cagtctatcc gtgaaaatct
    57541 tacctttggc cgtcaagata tttcagatga gtccatttgg aatttagctg agacttgtgg
    57601 tatgaaagac agactgttag cttgtaagga aggtttggat acgattatca agagtccttc
    57661 tgacttttca gctggagaag gtcaacgttt agagttgatg cgagctttat tgaaagatgc
    57721 gagttgctat atttttgatg aacccacgtc aaatttagat tctctaaatg aagctatctt
    57781 gcttgattta attaaaacac attgtcaagg attggtcttc ttaatttctc acaggccatc
    57841 aaccctagct tgtgctgatc atctattttg cgtaaaaaat ggttccctaa aagaggtgaa
    57901 taaaaaatga aacagttaac aataaaagat attatgataa caggcgcatt tgccgcgctc
    57961 tactttattt gtgttggtct aggcacgttt attaacatct tttttgatcg ctcaggaaat
    58021 atgttatatg cccctgcctt tgcagcttta ctggggggaa caatttattt tcttttgatt
    58081 gctaaattgc aaacatttgg gccaataaca ttattaggat tgatgatggg aggttttttc
    58141 ttccttagtg gtcacttttt tgccgcgatg tttcccagca ttattttcgg tttaatagcg
    58201 gatattattg cttctttagg aaaatacaag tcaaggtttt ttaatgacct atcttatatg
    58261 gtttttgctt ttgtcaatag tggccctatt gtgctgatgt ggttgttgaa agatgcctat
    58321 gttgaaagtt tgttagcccg cggtaaaagt atgacctatg tcaatcgcat tatgtatgat
    58381 tttagtttag ggcatgttat ttggtttgta ggaattgttc ttgcaggagc cttattaggg
    58441 tcagcttttg cacattattt actaaagaaa cattttatca aatcaggatt gttatcatga
    58501 ggcttgatgt tcgcaccaaa ctgcttttgt tggtacttgc caatgcctgc ttctttttcc
    58561 gtgttgatgg ctttttagaa tttataattg ttatttttct cttattactt ttgtctgctt
    58621 taaataagaa aaagctagca ttcaagttgg cggttgttta tctgttaatg atcggtctat
    58681 ctgtaatccc gctatctatt ttcccttctt atcttgatca cttattgagt tttgtctcaa
    58741 tagcaggaag gttagttttc ccttctttac tagcaggttt aattactatt aagacaacta
    58801 ccatctacga attggttcat ggattacgca aatggcattt cccagaagtc tggctattga
    58861 ccttagccgt tatgtgccgt ttcataccaa tgattagaca agaatgttgt gttattcatc
    58921 gctctttaac aatcaggggg attattttaa caaaatggtc tattttgatc agacctaaac
    58981 aatacttgga gtatttaatg gttccattgc ttttatcttt gataagaagt agccaagaac
    59041 taactatcgc tagtctaact aaagggctag cagtcaacaa aggcaccagt gaatgcttta
    59101 gctcacactt aacatggaaa gattgggggg tgcagatttg gattactgtt atcataatca
    59161 tcacgatact gcaatgattt cagcagaaca acttgttttt acctatcatg atcaaaaaaa
    59221 tccagcttgt caggtatcga cttgtcaaat tgcctctgga caattcattg ttttgtgtgg
    59281 accttctggt tcaggtaaat ctaccttttt aaaacttctt aatggcataa ttcctgatta
    59341 ttatgctgga aaatatgaag gtagactaga tgtggctgat tgccaagcag acagagacag
    59401 tgttgaaaca ttttctagat ctgtggcctc tgtttttcaa aatccagcca gtcagttttt
    59461 ctatcgcgaa gtccaacacg aactggtctt tccctgtgaa aatcaaggtt tagatgcaaa
    59521 ggttatcatg aagcgtcttt ggactttagc agaagatttt gcttttgcag agctccttaa
    59581 caaggatatg tttggattgt caggagggca aaaacaaaga gttgctattg caacggccat
    59641 tatgcaaggt actaatatta tgctttttga tgagccaact gctaacttag atagtgcagg
    59701 catagctgct gttaaagcct atcttaccca attaaaagca gcaggaaaaa ccattattgt
    59761 agccgagcat cgcttacatt acttgatgga tttggcagat aactttttct actttaaaaa
    59821 tggtagatta acagataagt taacaacaca agatttgcta gccttgacag atgaacaacg
    59881 gcaagatatg ggactacgtc gcttagactt gtctgaccta aaacctgtgt tagctggtaa
    59941 gattgagagt caacattatc gcccagatga tagcctgtgt attgagcatc taacagttag
    60001 agcgggaagc aaaatactta gatgtattga acagcttagt tttgctgcag gaagtatttc
    60061 tggcattaca ggatcaaacg ggcttggaaa atcgcaatta gtttattata tagctggtat
    60121 cttagacgat aaaaaagcaa ctattaagtt tcaaggcata cctttatctg ccaagcaacg
    60181 tttatcaaag actagtattg tcttacaaga agtttctttg caactatttg cagaatcggt
    60241 ttcaaaggaa gtcaacttgg gccatgaacg acatccacgg acgacagaag taatagagcg
    60301 gttatcttta acgactcttt tagaacgtca tccagctagt ttgtcaggag gtgagcaaca
    60361 aagagtgatg attgccgcta gtcttttggc tgataaagat atcttaattt ttgatgagcc
    60421 aagtagcgga cttgatttgt tacaaatgaa ggctttagct aatcttctca tgcagctgaa
    60481 gacccaacat aaggttgtca tcctcatttc acatgatgaa gaacttctaa gtagtgtctg
    60541 cgataccatt tatcaaatgc taccttaacg agcccctaga actgtttaaa agtactaaaa
    60601 ggcttttatc tgaaaataaa taggaactct tgtataatag atacaagagt cagaacgtag
    60661 acaactccag ttttatgctc aaaaagcatg catagctctg ctgatttgac attcggtttc
    60721 cgatttttac atataaagtg agccaaaaca gatgcttttt gctgtggtaa aagagtggcg
    60781 tcaatacgag attgacgcca ctcgggattt ttgagacctc aggctcaaaa ataaaggcat
    60841 ggaattccaa agaacgtcgc tgccgtaagc accacctaag gaaagtatca cgacaaactt
    60901 tgtcttcaat ctgacaagag tttttgtgaa gggaaaaaga taatgatatt aacagctcct
    60961 atgtccaact taaagggatt tggaccaaaa tcagcagaaa aatttcagaa attagatatt
    61021 tatacagtag aagatttact gctttattat ccgtttcgct atgaagattt taaatcaaaa
    61081 tctgtttttg atttagtgga tggtgaaaaa gcagtcatta caggcttagt cgttactcca
    61141 gctaatgtac aatattatgg ttttaaacgt aaccgtttaa gtttcaaatt gcgtcaaggg
    61201 gaagctgtct taaatgttag tttttttaat caaccctatt tagctgataa aatagaactt
    61261 ggtcaagagg tagctgtttt tggtaaatgg gatgccacta aatcggctat tactgggatg
    61321 aaggttttag ctcaagttga agatgacatg caacctgttt atcgcgtagc tcagggaatt
    61381 tcacagtcta ctttgattaa agctattaag tcagcttttg aaatcgatgc gcatttggaa
    61441 ttgaaggaaa atttaccagc tactttattg gaaaaatacc gattgatggg tcgtagtcag
    61501 gcttgtttag ctatgcattt cccaaaagat atcacagagt ataagcaagc gctccgtcgg
    61561 attaaatttg aagaattatt ttactttcaa atgaaccttc aagttttgaa agccgaaaat
    61621 agatctgaaa caaatggttt gcctattctt tatagtaaac atgctatgga gacaaagatt
    61681 tcctctttac cctttattct aacaaatgct caaaagcgct ctttagatga catattatct
    61741 gatatgtcat cgggagctca tatgaatcgt ttattgcaag gagatgtagg atcaggaaag
    61801 acagtcattg ctggtctatc aatgtatgca gcttatacag caggttttca atcggctttg
    61861 atggttccaa cggaaatcct agctgaacaa cactacatta gtctgcaaga gttatttcca
    61921 gatttatcaa tcgctatatt aacttcgggt atgaaagcag ctgtcaagcg tacggtttta
    61981 gcagctattg caaatggctc ggttgatatg attgtaggaa ctcatgctct tatccaagac
    62041 tcggtacagt accataaact ggggcttgtc attacagacg agcaacatcg ttttggtgtt
    62101 aaacagcgta gaattttccg tgaaaaggga gaaaatcctg atgttttaat gatgacagcc
    62161 accccaattc cccgaactct agcaatcaca gcttttggag aaatggatgt ttctattatt
    62221 gatgaattac ctgccggtcg taaacctatt atgacacgct gggtgaaaca cgagcagcta
    62281 ggtactgtgt tggaatgggt aaaaggtgaa ttgcaaaaag atgctcaagt gtatgtcatt
    62341 tcaccgttga ttgaagaatc agaagcttta gatttaaaga atgcagtagc attgcatgct
    62401 gaattatcta cttattttga aggaattgct aaggttgctc ttgtacatgg acgtatgaaa
    62461 aatgatgaaa aagatgctat aatgcaagat ttcaaggata aaaaaagtca tattttagta
    62521 tccacaacag ttattgaagt aggggtaaat gtcccaaatg caacgatcat gattattatg
    62581 gatgccgatc gttttggatt aagtcagtta catcaacttc gtgggcgtgt tggtcgtgga
    62641 tataaacaat catacgctgt tttagtggct aatcccaaaa ctgattcggg gaaaaaacga
    62701 atgacaatca tgacagaaac gacagatggt ttcgttttag ctgagtcgga tttaaaaatg
    62761 cgtggttctg gtgaaatctt tggtactcgt cagtctggaa ttccagaatt tcaagtagct
    62821 gatatcgttg aggattatcc tattttagaa gaagcacgca aagtttctgc agcgattgtt
    62881 tctgatccta actggatata tgaaaaacag tggcaattag tggcacaaaa tattagaaaa
    62941 aaagaagttt atgattaact taaaacaagt cgttgtttta caaatattta gttagttttg
    63001 agataaactg ttataaagta taaagaggtc actatttctg gcctttttta cttatgcttt
    63061 acgatgagac tgctatgtga agcagtatta accca
//
LOCUS       DATXIG010000006        53382 bp    DNA     linear   BCT 28-DEC-2023
DEFINITION  TPA_asm: Streptococcus pyogenes strain MGAS11113
            SAMN08555799-rid19682583.denovo.006, whole genome shotgun sequence.
ACCESSION   DATXIG010000006 DATXIG010000000
VERSION     DATXIG010000006.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN08555799
            Sequence Read Archive: SRR6740495
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pyogenes
  ORGANISM  Streptococcus pyogenes
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 53382)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 53382)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-OCT-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-OCT-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001918058.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001918058.2
            Genome Coverage       :: 368x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/12/2023 11:23:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 1,806
            CDSs (total)                      :: 1,769
            Genes (coding)                    :: 1,722
            CDSs (with protein)               :: 1,722
            Genes (RNA)                       :: 37
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1, 1 (5S, 23S)
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 47
            CDSs (without protein)            :: 47
            Pseudo Genes (ambiguous residues) :: 0 of 47
            Pseudo Genes (frameshifted)       :: 30 of 47
            Pseudo Genes (incomplete)         :: 20 of 47
            Pseudo Genes (internal stop)      :: 9 of 47
            Pseudo Genes (multiple problems)  :: 11 of 47
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..53382
                     /organism="Streptococcus pyogenes"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN08555799-rid19682583.denovo.006"
                     /strain="MGAS11113"
                     /serotype="Emm28"
                     /isolation_source="normally sterile site"
                     /host="Homo sapiens"
                     /db_xref="taxon:1314"
                     /country="Finland"
                     /collection_date="2002"
                     /collected_by="National Public Health Laboratory"
     gene            141..1337
                     /gene="tuf"
                     /locus_tag="VC122_000361"
     CDS             141..1337
                     /gene="tuf"
                     /locus_tag="VC122_000361"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014335040.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor Tu"
                     /protein_id="HEP5640315.1"
                     /translation="MAKEKYDRSKPHVNIGTIGHVDHGKTTLTAAITTVLARRLPSSV
                     NQPKDYASIDAAPEERERGITINTAHVEYETATRHYAHIDAPGHADYVKNMITGAAQM
                     DGAILVVASTDGPMPQTREHILLSRQVGVKHLIVFMNKVDLVDDEELLELVEMEIRDL
                     LSEYDFPGDDLPVIQGSALKALEGDTKFEDIIMELMDTVDSYIPEPERDTDKPLLLPV
                     EDVFSITGRGTVASGRIDRGTVRVNDEIEIVGIKEETKKAVVTGVEMFRKQLDEGLAG
                     DNVGILLRGVQRDEIERGQVIAKPGSINPHTKFKGEVYILSKDEGGRHTPFFNNYRPQ
                     FYFRTTDVTGSIELPAGTEMVMPGDNVTINVELIHPIAVEQGTTFSIREGGRTVGSGI
                     VSEIEA"
     gene            1578..2336
                     /locus_tag="VC122_000362"
     CDS             1578..2336
                     /locus_tag="VC122_000362"
                     /EC_number="5.3.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012515922.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="triose-phosphate isomerase"
                     /protein_id="HEP5640316.1"
                     /translation="MSRKPIIAGNWKMNKNPQEAKAFVEAVASKLPSTDLVDVAVAAP
                     AVDLVTTIEAAKDSVLKVAAQNCYFENTGAFTGETSPKVLAEMGADYVVIGHSERRDY
                     FHETDEDINKKAKAIFANGLTPIVCCGESLETYEAGKAVEFVGAQVSAALAGLSAEQV
                     ASLVLAYEPIWAIGTGKSATQDDAQNMCKAVRDVVAADFGQEVADKVRVQYGGSVKPE
                     NVKDYMACPDVDGALVGGASLEADSFLALLDFLN"
     gene            complement(2435..3670)
                     /locus_tag="VC122_000363"
     CDS             complement(2435..3670)
                     /locus_tag="VC122_000363"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002990536.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminoacyltransferase"
                     /protein_id="HEP5640317.1"
                     /translation="MALIEISQEQFDHYCHSLVHHSFIQTSEMASLMAKRGAKPQFLG
                     LEKDGELKVAAMVFSQKVAGGWRMELNAGPNTNHPEELEHFYTQLKDYAKQNDVIELI
                     LKPYDNYQSFDTDGIPISHPNTDLISLLTALGYKHDGLKTGYPEGEPVWHYVKKLEGI
                     DSSRLTHSFSKKGKALIKKANTFGIKLRQLKRDELHHFKEITEATSDRRDYLDKSLSY
                     YQDFYDSFGDSCEFMVATLNFEDYLNNLKQRQLQLATSINKVKGDLGKNPHSEKKQNR
                     LKELSSQFETFQVRISEALHFLEEYGTKDVFLAGSLFIYTEQEAVYLFSGSYPKFNKF
                     YSPALLQEHAMLKAIHKGIKQYNFLGITGKFDGSDGVLRFKQNFNGFILQKPGTFRCY
                     PFPIKYHFIRLAKKLLNRY"
     gene            complement(3657..4883)
                     /locus_tag="VC122_000364"
     CDS             complement(3657..4883)
                     /locus_tag="VC122_000364"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012560553.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipid II:glycine glycyltransferase FemX"
                     /protein_id="HEP5640318.1"
                     /translation="MYTYKIGISPEEHDQFVLAQPQAGLLQSSKWRKVKDNWKHERIS
                     FYENGVQVAAAACLIRKLPLGFTMIYIPRGPIMDYANFELLDFVIKTLKTFGKSKRAL
                     FVKIDPSLVIKQTLEGKESKENDVTLSIIAFLKKLGIEWSGRTKELEDTIQPRIQANI
                     YAKDFDFDSLPKKAKQSIRTATNKGVNVTIGGSELLDDFSALMKKTENRKGIILRGKS
                     YYQKLLDIYAGQSYITMASLDLPEQKKLLIQQLDKALAEQARLTDKSKPSKVAENQKT
                     IARLQKDLTILSEQLAAGKTRIPLAATLTLIYGETSENLYAGMDDDYRNYQAPLLTWY
                     ETAKEAFKRGCRWHNLGGVENQQDGGLYHFKARLNPTIEEFAGEFNIPVGLVSSLAIL
                     TYNLRKKLRSTTNGTN"
     gene            complement(4883..5692)
                     /gene="yidA"
                     /locus_tag="VC122_000365"
     CDS             complement(4883..5692)
                     /gene="yidA"
                     /locus_tag="VC122_000365"
                     /EC_number="3.1.3.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002985513.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar-phosphatase"
                     /protein_id="HEP5640319.1"
                     /translation="MSIKLVAVDIDGTLLTDDRRITDDVFQAVQEAKAQGVHMVIATG
                     RPIAGVISLLEQLELNHKGNHVITFNGGLVQDAETGEEIVKELMTYDDYLETEFLSRK
                     LGVHMHAITKEGIYTANRNIGKYTVHESTLVNMPIFYRTPEEMTNKEIIKMMMIDEPD
                     LLDAAIKQIPQHFFDKYTIVKSTPFYLEFMPKTVSKGNAIKHLAKKLGLDMSQTMAIG
                     DAENDRAMLEVVANPVVMENGVPELKKIAKYITKSNNDSGVAHAIRKWVLN"
     gene            complement(6148..7449)
                     /locus_tag="VC122_000366"
     CDS             complement(6148..7449)
                     /locus_tag="VC122_000366"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009880543.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HD domain-containing protein"
                     /protein_id="HEP5640320.1"
                     /translation="MNEKVFRDPVHNYIHIDNPLIYDLINTKEFQRLRRIKQVPTTAF
                     TFHGAEHSRFSHCLGVYEIARRVTAIFEEKYADIWNKDESLVTMTAALLHDIGHGAYS
                     HTFEVLFHTDHEAFTQEIITNPETEINAILVRHALDFPDKVASVINHTYPNKQVVQLI
                     SSQIDCDRMDYLLRDSYFSAANYGQFDLMRILRVIRPVEDGIVFEHSGMHAVEDYIVS
                     RFQMYMQVYFHPASRAVELILQNLLKRAQHLYPEQQAYFQKTAPGLIPFFEKKANLAD
                     YIALDDGVMNTYFQVWMASEDHILSDLASRFINRKILKSVTFDQDSRGELERLRQLVE
                     SVGFDPDYYTGIHINFDLPYDIYRPELENPRTQIEMIQKDGSLAELSQLSPIVKALTG
                     TTYGDRRFYFPKEMLELDDLFAPSKETFMSYISNGHFHFSQ"
     gene            7531..7917
                     /locus_tag="VC122_000367"
     CDS             7531..7917
                     /locus_tag="VC122_000367"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011528418.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1934 domain-containing protein"
                     /protein_id="HEP5640321.1"
                     /translation="MKLQLTNHIRFGDETEIIQEIHDCEWREKGGYQYLIYQNTDKEK
                     VVIKYNETELTMSRFSNPQSIMKFFAGKKVLIALPTPMGVQQFLTDTSHYHLDCSCQK
                     LDLHYHLLQAQTEMLFASYHLELSWD"
     gene            8147..10828
                     /locus_tag="VC122_000368"
     CDS             8147..10828
                     /locus_tag="VC122_000368"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010922034.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cation-translocating P-type ATPase"
                     /protein_id="HEP5640322.1"
                     /translation="MSKEQRHEAFYTQSEETVLAQLETSREGLTSAQAKERLAEYGRN
                     ELDEGEKRSLFMKFLDQFKDLMIIILIVAALLSVLTEGMEGLTDAIIILAVVILNAVF
                     GVYQEGQAEAAIEALKSMSSPLARIRRDGHVTEIDSKELVPGDIVLLEAGDVVPADLR
                     LLEANSLKIEEAALTGESVPVEKDLSTAVSEDAGIGDRVNMGYQNSNVTYGRGIGVIT
                     NTGMYTEVGHIAGMLANADETDTPLKQNLDNLSKILTYAILVIAAVTFAVGVFLRGQH
                     PLEGLMTSVALAVAAIPEGLPAIVTVVLSLGTQVLAKRNAIIRKLPAVETLGSTEIIA
                     SDKTGTLTMNQMTVEKVYTNGTLQSSSADIAFDNTTLRVMNFANDTKVDPSGKLIGDP
                     TETALVQFGLDHNFDVREAMVAEPRVAELPFDSDRKLMSTIHKQADGKYFIAVKGAPD
                     QLLKRVTQIEENGQIRPITDADKKTILDTNKSLAKQALRVLMMAYKYSDALPTLETEI
                     VEANLVFSGLVGMIDPERPEAAQAVKVAKEAGIRPIMITGDHQDTAKAIAKRLGIIEE
                     DGVDHVFTGAELNELSDEEFQKVFKQYSVYARVSPEHKVRIVKAWQNEGKVVAMTGDG
                     VNDAPSLKTADIGIGMGITGTEVSKGASDMVLADDNFATIIVAVEEGRKVFSNIQKTI
                     QYLLSANMAEVFTIFLATLFGWDVLQPVHLLWINLVTDTLPAIALGVEPAEPGVMKHK
                     PRGRKSSFFDGGVKEAILYQGAFQTILVLGVYGFALMFPEHTSYHDVHADALTMAYVT
                     LGLIQLVHAYNVKSVYQSIFTVGLFKNKLFNYSIPVAFVALMATVVVPGFNQFFHVTH
                     LTITQWLVVIIGSLLMVVLVELVKAVQRSLGQDEKAI"
     gene            complement(10912..11907)
                     /locus_tag="VC122_000369"
     CDS             complement(10912..11907)
                     /locus_tag="VC122_000369"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010922035.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HEP5640323.1"
                     /translation="MQRKVTIKDIAELAKTSKTTVSFYLNGRFDKMSEETKNRIKSTI
                     EATNYKPSIAARSLNAKSTKLIGVVIGDITNSFSNQIVKGIESKAQEFGYQIIIGNSN
                     YDPSREDELIEKMLNLGVDGFIIQPTSNFRKYSRIIDIKKKKVVFFDSQLYEHRTNWV
                     KTNNYDAVYDTIQQCIDKGYEHFIMITGNPNLLSTRIERASGFIDVLEANHLTHQEMI
                     IDENQTSSEAIAQFLQGSLTKKSLVFVPNCWALPKVFTAMKSLKFNIPEIGLVGFDNI
                     EWTKFSSPTLTTIIQPAYEEGEQATKILIDDIEGHSQEAKQQIFDCQVNWQESTF"
     gene            complement(11971..13878)
                     /locus_tag="VC122_000370"
     CDS             complement(11971..13878)
                     /locus_tag="VC122_000370"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002995224.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alginate lyase family protein"
                     /protein_id="HEP5640324.1"
                     /translation="MSLAFTRFKETVNPNFCRNYLLDYQADSYADRKRIADLLLTNTF
                     LFEDNWDMEPCHIPYHLDPITWQEAVTDDPEWNFMLNRQTYLQTLILVYLVERDERYL
                     LTAKGFILNWIESAIPLDPKGLATRTLDTGIRCFAWVKCLIYLNLFDALTKQEERLIL
                     ASLEKQLHFLHANYLDKYSLSNWGILQTTAILLADAYFGSDLDIAAATAFARKELTQQ
                     IALQILEDGSQFEQSTMYHVEVLKALLELTALVPDYLPQLRPTLLAMSDYLLKMTGPD
                     HKQIPLGDSDFTDTRDILTLAATILEEPHLKAAAFPTLDIDSLLLLGEKGVHTFEQLP
                     VQTLPTFAHHFEHSGHITINQENYYLFFKNGPIGSSHTHSDQNSICLYYKGQPLFCDA
                     GRYTYKEEPLRYALKSASHHSTAFLEEQLPEQINSSWAYLSYPKSNYCHLRQNGHVYF
                     IEGSYQTQFSDRNNYQHDRQILILPPGMFLIIDTIQAQGNHCLVSQFILDNHLEVKTD
                     HLSDLRLISDCPFTIEETILSKKYNQYLTSHKFIKRKPFKDKGCTSTLLVPDDTKVTP
                     LTPLQTGKRNPIETALSWHLKGKQFDYSICVLQEDLIKGEKLVLLNGHKIRGKVVVIN
                     HITNEIIRLKH"
     gene            complement(13964..14785)
                     /locus_tag="VC122_000371"
     CDS             complement(13964..14785)
                     /locus_tag="VC122_000371"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011889050.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS mannose/fructose/sorbose transporter family
                     subunit IID"
                     /protein_id="HEP5640325.1"
                     /translation="MTGSNKLTKKDYLKTALRAFFLQNGFNYSNYQGIGYANVIYPAL
                     KKHFGSDKKGLYQALEDNCEFYNTNPHFLPFITSLHLVMLENDRPEEETRNIKMALMG
                     PLAGIGDSLSQFCLAPLFSTIAASLASDGLVLGPIFFFLAMNIILTAIKIGSGLYGYK
                     VGTSFIDKLSEQMAIVSRMANIVGVTVIAGLAATSVKITVPITFAAGKVDAANTAQKF
                     VTIQGMLDKIAPALLPALFTLLMYYLIKNKKWTTYKLVILTVIIGVIGSWLGILA"
     gene            complement(14772..15554)
                     /locus_tag="VC122_000372"
     CDS             complement(14772..15554)
                     /locus_tag="VC122_000372"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003047650.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS
                     mannose/fructose/sorbose/N-acetylgalactosamine transporter
                     subunit IIC"
                     /protein_id="HEP5640326.1"
                     /translation="MDINLLQALLIGLWTAFCFSGMLLGIYTNRCIILSFGVGMILGD
                     LPTALSMGAISELAYMGFGVGAGGTVPPNPIGPGIFGTLMAITSAGKVTPEAALALST
                     PIAVVIQFLQTFAYTAFAGAPETAKKQLQKGNIRGFKFAANGTIWAFAFMGLGLGLLG
                     ALSMDTLLHLVDYIPPVLLNGLTVAGKMLPAIGFAMILSVMAKKELIPFVLIGYVCAA
                     YLQIPTIGIAIIGIIFALNEFYNKPKQVDATTVQGGQQDDWI"
     gene            complement(15573..16061)
                     /locus_tag="VC122_000373"
     CDS             complement(15573..16061)
                     /locus_tag="VC122_000373"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002990518.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS system
                     mannose/fructose/N-acetylgalactosamine-transporter subunit
                     IIB"
                     /protein_id="HEP5640327.1"
                     /translation="MTQPNIIMTRVDERLIHGQGQLWVKFLNCNTVIVANDAVSEDKI
                     QQSLMKTVIPSSIAIRFFSIQKVIDIIHKASPAQSIFIVVKDLQDAKRLVEGGVPITE
                     INIGNIHKTDDKVAITQFISLGETDKSAIRCLAHDHHVVFNTKTTPAGNSASDVDILD
                     YI"
     gene            complement(16097..17296)
                     /locus_tag="VC122_000374"
     CDS             complement(16097..17296)
                     /locus_tag="VC122_000374"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015016828.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 88 protein"
                     /protein_id="HEP5640328.1"
                     /translation="MTRSLKTIALEPIKQPERFTKEDFLSQEDITQALDLALKQVRLN
                     MDYFKEDFPTPATKDNQYAIMDNTEWTNAFWTGCLWLAYEYGGDDAIKALAQANDLSF
                     LDRVTRDIELDHHDLGFLYTPSCMAEWKLLKTPESREAALKAADKLIQRYQDKGGFIQ
                     AWGVLGKKEDYRLIIDCLLNIQLLFFASQETGDNRYRDMAINHFYASANHVIRDDASA
                     YHTFYFDPETGDPIKGVTRQGYSDDSAWARGQAWGIYGIPLTYRFLKEPELIQLFKGM
                     THYFMNRLPKDQVSYWDLIFGDGSEQSRDSSATAIAVCGIHEMLKTLPDHDPDKKTYE
                     AAMHSMLRALIKDYANKNLNPGAPLLLHGVYSWHSGKGVDEGNIWGDYYYLEALLRFY
                     KDWNPYW"
     gene            complement(17296..17733)
                     /locus_tag="VC122_000375"
     CDS             complement(17296..17733)
                     /locus_tag="VC122_000375"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003050049.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HEP5640329.1"
                     /translation="MIAIIVMGHGHFASGIVSSLELIAGPQEKVTAIDFTTEMTAADV
                     QDQLSRALIPEEETLVLCDLLGGTPFKVAATLMESLPNTTCNVLSGLNLAMLIEASFA
                     RQTAASFDDLVSGLITCSKEGIVDWKTLSQQEDGTTDDELGGI"
     gene            18068..18862
                     /locus_tag="VC122_000376"
     CDS             18068..18862
                     /locus_tag="VC122_000376"
                     /EC_number="1.1.1.69"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012766735.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gluconate 5-dehydrogenase"
                     /protein_id="HEP5640330.1"
                     /translation="MENMFSLQGKIALITGASYGIGFEIAKAYAQAGATIVFNDIKQE
                     LVDKGVAAYRELGIEAHGYVCDVTDEAGIQQMVSQIEDEVGVIDILVNNAGIIRRTPM
                     LEMAAEDFRQVIDIDLNAPFIVSKAVLPSMIAKGHGKIINICSMMSELGRETVSAYAA
                     AKGGLKMLTKNIASEFGEANIQCNGIGPGYIATPQTAPLRERQADGSRHPFDQFIIAK
                     TPAARWGTTEDLAGPAVFLASDASNFVNGHILYVDGGILAYIGKQP"
     gene            18887..19528
                     /locus_tag="VC122_000377"
     CDS             18887..19528
                     /locus_tag="VC122_000377"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010922043.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RpiB/LacA/LacB family sugar-phosphate isomerase"
                     /protein_id="HEP5640331.1"
                     /translation="MKIALINENSQAAKNGIIYDALTSVTDKHGYQVFNYGMYGTEGE
                     SQLTYVQNGLLASILLTTKAADFVVTGCGTGVGAMLALNSFPGVTCGFASEPTEAYLF
                     SQINGGNALSIPFAKGFGWGAELNLTLIFERLFAEPMGGGYPKERAIPEQRNARILSD
                     LKKITYRDLLAIVKDIDQDFLKETISGAHFKEYFFANAEPSELVAYLKSVLEQ"
     gene            19557..20558
                     /locus_tag="VC122_000378"
     CDS             19557..20558
                     /locus_tag="VC122_000378"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012560559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar kinase"
                     /protein_id="HEP5640332.1"
                     /translation="MSKLLLVGEPLIRVSPNQFQPLTNACDAQLFFGGSEANIARTLG
                     GFGLEARLFTALPDNPVGHAFHQFLKQSGVDMSLTAWQGHRVGLYYLENGFGCRASQV
                     YYDRCGSSFSALDKDSLDLAAIFEGISHFHFSGISLALGKKTQDLIEILAREARKRQV
                     CVSFDLNFRSSMIAVADAKRLFSHFAQYADIIFGMEPLLLDSDDFDMFDRNKADTTMI
                     RERLAGLYQRYQLQAIYHTERSNDAQGSNRFKAYAYDGQFYESCEVTTPVLQRVGSGD
                     AFVAGLLYQLLEGNEKQRNLDFAVATASLKCTVAEDQLYHSVQQVEAVLHNQRDVQR"
     gene            20563..21198
                     /locus_tag="VC122_000379"
     CDS             20563..21198
                     /locus_tag="VC122_000379"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011017554.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional 4-hydroxy-2-oxoglutarate
                     aldolase/2-dehydro-3-deoxy-phosphogluconate aldolase"
                     /protein_id="HEP5640333.1"
                     /translation="MGKIEILTKLKANRLVLVVRGESSEEALACSLASIEGGIKTIEV
                     TYTNPFASEVIGQLAERFKEDPEVLIGAGTVLDDVTARQAILAGAQFIVGPNFNRAVA
                     LICHGYSIPYLPGCMTVNEVVTALESGVDMVKIFPGSTVGISFIRAIKSPLPQVEVMV
                     TGGVSSDNLKDWLAAGVDVLGIGGEFNQLASQKQYNLITKKAAHYIKSLRQ"
     gene            21494..22144
                     /locus_tag="VC122_000380"
     CDS             21494..22144
                     /locus_tag="VC122_000380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002994445.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family phosphatase"
                     /protein_id="HEP5640334.1"
                     /translation="MIKGIIFDMDGVLFDTEPFYLRRREDFFKTKGIPIDHLNSKDFI
                     GGNLQELWKELLGKNRDDAIVKAITTDYDAYKQAHKPPYQKLLITEVNSCLEQLKKQG
                     IKLAVASNSKRQDVLLALETTQIKDYFEIILAREDVSRGKPYPDIYNKAVQKLGLQKK
                     QLLVVEDSEKGIAAAKAANLTVFAITDYRYGIDQSQADHKIDHLGQLCVKIGCFGS"
     gene            22841..23965
                     /gene="queG"
                     /locus_tag="VC122_000381"
     CDS             22841..23965
                     /gene="queG"
                     /locus_tag="VC122_000381"
                     /EC_number="1.17.99.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011054310.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA epoxyqueuosine(34) reductase QueG"
                     /protein_id="HEP5640335.1"
                     /translation="MTIKAEIKALAKEIGISKIGFTTADNFDYLEKSLRASVEEGRNS
                     GFEHKVMEDRIYPERLLESAKTIISIGVAYPHKLPQQPQKTPYKRGKITPSSWGLDYH
                     YVVGEKLDRLSKGIEELCRDFPLQQKAMVDTGALVDTAVAQRAGIGFIGKNGLVISKE
                     YGSYMFLGELITNLEIEPDKPVDYDCGDCRRCLDACPTSCLIGDGSMNAKRCLSFQTQ
                     DKGMMDIEFRKKIKTVIYGCDICQICCPYNKGINNSPATEIDPELAQPELIPFLSLSN
                     GQFKEKFGMIAGGWRGKNILQRNAIIALANAHDKTAVVKLIEIIDKNNNPIHTATAIW
                     ALGEIVKKPNDEMLAFMSHLTLKDEDSQKELELIRRKWQV"
     gene            24034..25132
                     /gene="prfB"
                     /locus_tag="VC122_000382"
     CDS             join(24034..24105,24107..25132)
                     /gene="prfB"
                     /locus_tag="VC122_000382"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011889043.1"
                     /ribosomal_slippage
                     /note="programmed frameshift; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor 2"
                     /protein_id="HEP5640336.1"
                     /translation="MEVAEIRQKIVENKEKLTSFRRSLDLDRLEEEIALLENHMTEPD
                     FWNDNIAAQKTSQELNELKGKYDTFHNMQELSDETELLLEMLDEDDSLKEELEENLMQ
                     LDKIMGAYEMTLLLSEPYDHNNAILEIHPGSGGTEAQDWGDLLLRMYTRFGNANGFKV
                     EVLDYQAGDEAGIKSVTLSFEGPNAYGLLKSEMGVHRLVRISPFDSAKRRHTSFASVE
                     VMPELDDTIEVEVRDDDIKMDTFRSGGAGGQNVNKVSTGVRLTHIPTGIVVSSTVDRT
                     QYGNRDRAMKMLQAKLYQLEQEKKAQEVDALKGDKKEITWGSQIRSYVFTPYTMVKDH
                     RTNFELAQVDKVMDGEINGFIDAYLKWRIED"
     gene            25151..25843
                     /gene="ftsE"
                     /locus_tag="VC122_000383"
     CDS             25151..25843
                     /gene="ftsE"
                     /locus_tag="VC122_000383"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014294955.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division ATP-binding protein FtsE"
                     /protein_id="HEP5640337.1"
                     /translation="MALIEMSGVTKKYRRSTTALRDVNVSVNQGEFVYLVGPSGAGKS
                     TFIKLLYREEQLTTGKLYVGEFNLTKLKARDVPILRRHIGVVFQDYKLLPRKTVFENV
                     AYAMEVIGEKRRHIKKRVPEVLDLVGLKHKMRSFPSQLSGGEQQRVAIARAIVNNPKL
                     LIADEPTGNLDPEISWEIMQLLERINVQGTTILMATHNSHIVNTFRHRVVAIEDGRIV
                     RDEEKGDYGYDD"
     gene            25836..26765
                     /locus_tag="VC122_000384"
     CDS             25836..26765
                     /locus_tag="VC122_000384"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002990498.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEP5640338.1"
                     /translation="MIRYFFRHIWESIKNLKRNFWMTFASVSMVAVTLTLVGVFAATL
                     LNIQRVASGVENNVHINTYLQVDSTDAAKVIQNTAGEPVNNDNYHSVYDKIAQIKGVK
                     KITFSSKDEQLKKLQETLGDVWNMYDQDTNPLQDIYLIETQTPKQVKAITKKIRTIEG
                     VEAADYGGINSDKLFKFSTLIQTWGLIGTAMLLFVAVFLISNTIRMTIMSRKRDIEIM
                     RLVGAKNSYIRGPFFFEGAWVGLLGAVLPSLLIYYGYDLVYKHFAQELQRNNLSMYPL
                     DPYVYYLIGALFVIGIMIGSLGSVLSMRRYLKF"
     gene            complement(27075..27773)
                     /locus_tag="VC122_000385"
     CDS             complement(27075..27773)
                     /locus_tag="VC122_000385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011017557.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HEP5640339.1"
                     /translation="MPFIFRHSFFNKALIFWYTILMKIYKTINHIAGENTYYLVNDQA
                     VILIDPGSNGQEIIAKIKSFEKPLVAILLTHTHYDHIFSLDLVRDAFDHPPVYVSEKE
                     AAWLSSPDDNLSGLGRHDDIITVIARPAENFFKLKQPYQLNGFEFTVLPTPGHSWGGV
                     SFVFHSDELVVTGDALFRETIGRTDLPTSNFEDLITGIRQELFTLPNHYRVYPGHGPS
                     TTICHEKNANPFFH"
     gene            27941..28705
                     /locus_tag="VC122_000386"
     CDS             27941..28705
                     /locus_tag="VC122_000386"
                     /EC_number="1.1.1.304"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000048144.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="(S)-acetoin forming diacetyl reductase"
                     /protein_id="HEP5640340.1"
                     /translation="MSKVAIVTGAGQGIGFAIAKRLHADGFKIGILDYNEETAQAAVD
                     KLSPEDTVAVVADVSKRDQVFDAFQKVVDTFGDLNVVVNNAGVAPTTPLDTITEEQFE
                     KAFAINVGGTIWGSQAAQKHFRELGHGGKIINATSQAGCEGNPNLTVYGGTKFAVRGI
                     TQTLAKDLASEGITVNAYAPGIVKTPMMFDIAHEVGKNAGKDDEWGMEQFAKDITLKR
                     LSEPEDVANAVGFLVGDDSNYITGQTIVVDGGMVFH"
     gene            28813..31314
                     /locus_tag="VC122_000387"
     CDS             28813..31314
                     /locus_tag="VC122_000387"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011528429.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional DnaQ family
                     exonuclease/ATP-dependent helicase"
                     /protein_id="HEP5640341.1"
                     /translation="MFCFIDIACYNRLTMTQKKLRKYAVVDLEATGAGPNASIIQVGI
                     VIIQGNKIIDSYETDVNPHESLDEHIVHLTGITDKQLAKAPDFGQVAHHIYQLIEDCI
                     FVAHNVKFDANLLAEALFLEGYELTIPRVDTVELAQLFFPRFEKYNLSHLSRQLNIDL
                     AEAHTAIADARATAILFLRLLQKIESLPIECLESLLAYSDSLLFETAMVIQEGLAKAK
                     PYDPNKYIKIRQILLPKGSKALKPYQISKSFPINMALLGLEERPKQTQFAQLIDEDYH
                     QGVASFIEAQTGIGKTYGYLLPLLAKEDQNQIIVSVPTKLLQDQLMAGEVAAIQEQFH
                     IACHSLKGPANYLKLDSFADSLDQNDQNRLVNRYKMQLLVWLLETKTGDLDEIKQKQR
                     FAAYFEQLKHDGDIKQSSEFYDYDFWRVSYEKAKTARLLITNHAYFLHRVQDDKDFTR
                     NKVLVFDEAQKLMLQLDQLSRHQLNLTVFLQTIQAKLSNPLPLLEKRLLESLSFELGQ
                     VSSDYYQNKEHQLAHDWSRIAGYAKELTGADYQELQAFFATSDGDYWLSSEKQEEKRV
                     TYLNSASKAFIHFQQLLPETVKTYFVSATLTISSEVTLADLLGFEEYLYHVIEKDKKQ
                     DQLVLVDQEAPIVTEVSDQIYVEAIAKRIESLKQEGYPILVLFNSKKHLLLVSDYLDQ
                     WQVPHLAQEKNGTAYNIKKRFDQGEQTILLGLGSFWEGVDFIQADRMITLIARLPFDN
                     PEDFFVKKMSHYLLEKGKNPFRDYFLPMTILRLKQAIGRTMRRQDQKSVVIILDRRLL
                     TKSYGQVILEGLGQEFLISQQNFHDCLVETDCFLI"
     gene            31649..32842
                     /locus_tag="VC122_000388"
     CDS             31649..32842
                     /locus_tag="VC122_000388"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000221826.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal phosphate-dependent aminotransferase"
                     /protein_id="HEP5640342.1"
                     /translation="MPKLSKRVLEMKESVTLAAGARAKALKAQGRDVLNLTLGEPDFF
                     TPKHIQDKAIESIQNGTASFYTNASGLPELKAAIATYLKNQYGYHLSPDQIVAGTGAK
                     FILYAFFMAVLNPGDQVLIPTPYWVSYSDQVKMAEGQPIFVQGLEENQFKVTVDQLER
                     ARTSKTKVVLINSPSNPTGMIYGAEELRAIGEWAVHNDILILADDIYGSLVYNGNQFV
                     PISTLSEAIRRQTITVNGVAKSYAMTGWRVGFAAGEPEIISAMSKIIGQTTSNLTTVS
                     QYAAIEAFCGSQSSLEEMRLAFEERLNITYPLLCQVPGFEVVKPQGAFYFFPNVKKAM
                     EMTGFSDVTSFANAILEEVGLAVVSGAGFGAPENVRLSYATDIETLKEAVRRLHVFME
                     SNEIN"
     gene            32872..34218
                     /gene="asnS"
                     /locus_tag="VC122_000389"
     CDS             32872..34218
                     /gene="asnS"
                     /locus_tag="VC122_000389"
                     /EC_number="6.1.1.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000038477.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="asparagine--tRNA ligase"
                     /protein_id="HEP5640343.1"
                     /translation="MSKKLISIVDVKDYVGQEVTIGAWVANKSGKGKIAFVQLRDGSA
                     FFQGVAFKPNFIEKYGEESGLEKFDVIKRLNQETSVYVTGIVKEDERSKFGYELDITD
                     LEIIGESHEYPITPKEHGTDFLMDNRHLWLRSRKQMAVMQIRNAIIYATYEFFDQNGF
                     IKFDSPILSENAAEDSTELFETDYFGKPAFLSQSGQLYLEAGAMALGRVFDFGPVFRA
                     EKSKTRRHLTEFWMMDAEYSFLSHEESLDLQEAYVKALIQGVLDRAPQALDILERDVE
                     ALKRYITEPFKRVSYDDAITLLQEHEADEDTDYEHLEHGDDFGSPHETWISNYFGVPT
                     FVVNYPASFKAFYMKPVPGNPERVLCADLLAPEGYGEIIGGSMREDNYDALVAKMDEL
                     GMDKSEYDFYLDLRKYGSVPHGGFGIGIERMVTFVAGTKHIREAIPFPRMLHRIRP"
     gene            34633..35523
                     /gene="rapZ"
                     /locus_tag="VC122_000390"
     CDS             34633..35523
                     /gene="rapZ"
                     /locus_tag="VC122_000390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002282100.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNase adapter RapZ"
                     /protein_id="HEP5640344.1"
                     /translation="MSDKHINLVIVTGMSGAGKTVAIQSFEDLGYFTIDNMPPALVPK
                     FLELIEQTNENRRVALVVDMRSRLFFKEINSTLDSIESNPSIDFRILFLDATDGELVS
                     RYKETRRSHPLAADGRVLDGIRLERELLSPLKSMSQHVVDTTKLTPRQLRKTISDQFS
                     EGSNQASFRIEVMSFGFKYGLPLDADLVFDVRFLPNPYYKVELREKTGLDEDVFNYVM
                     SHPESEVFYKHLLNLIVPILPAYQKEGKSVLTVAIGCTGGQHRSVAFAHCLAESLATD
                     WSVNESHRDQNRRKETVNRS"
     gene            35520..36497
                     /locus_tag="VC122_000391"
     CDS             35520..36497
                     /locus_tag="VC122_000391"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011284642.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YvcK family protein"
                     /protein_id="HEP5640345.1"
                     /translation="MKNPKMTVIGGGTGISIILKSLRNEAVDITAVVTVADDGGSSGE
                     LRNAMQLTPPGDLRNVLLAMSDMPKFYERVFQYRFNESDGALAGHPLGNLIIAGISEM
                     QGSTYNAIQILTKFFHITGKIYPSSEQALTLHAVFKDGHEVAGESSIAKYQGMIDHVY
                     VTNTYNDQKPQASRKVVEAILESDMIVLGPGSLFTSILPNLVIPEIKEALRQTKAEVV
                     YICNIMTQYGETEQFSDADHVAVLNQHLGRDLIDTVLVNVAKVPQAYMNSNKFDEYLV
                     QVDHDFAGLCRAAKRVISSYFLRLENGGAFHDGNLVVEELMNLVRIVKQ"
     gene            36494..37405
                     /gene="whiA"
                     /locus_tag="VC122_000392"
     CDS             36494..37405
                     /gene="whiA"
                     /locus_tag="VC122_000392"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003062628.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding protein WhiA"
                     /protein_id="HEP5640346.1"
                     /translation="MSFTTKVKEELIHLSTGDNNELAAIIKLSGSLGLAHQSLHLSIT
                     TENAKIARYIYSLIEDAYVIVPEIRYHQKTNLRKNRVYTVYVEQGVETILADLKLADS
                     FFGLETGIEPQVLSDDNAGRSYLKGAFLAAGSIRDPESGKYQLEIYSVYLDHAQDLAQ
                     LMQKFMLDAKTIEHKSGAVTYVQKAEDIMDFLIIIGAMSCKEDFEAIKLLREARNDIN
                     RANNAETANIAKTISASMKTINNIIKIMDTIGLESLPIELQQVAQLRVKHPDYSIQQV
                     ADALEFPITKSGVNHRLRKINKIADDL"
     gene            37538..38935
                     /locus_tag="VC122_000393"
     CDS             37538..38935
                     /locus_tag="VC122_000393"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012766747.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="C69 family dipeptidase"
                     /protein_id="HEP5640347.1"
                     /translation="MACTTILVGKKASYDGSTMVARTEDSQNGDFTPKKMIVVKPEDQ
                     PRHYRSVQSSFEMDLPDNPMTYTSVPDALGKDGIWAEAGVNEANVAMSATETITTNSR
                     VLGADPLVASGIGEEDMVTLVLPYIRSAREGVLRLGAILEDYGTYESNGVAFSDEHDI
                     WWLETIGGHHWIARRVPDDAYVTNPNQFGIDYFEFNNPEDYLCSADLKDFIDTYHLDL
                     TYNHEHFNPRYAFGSQRDKDRHYNTPRAWIMQKFLNPEIVQDPRSFALAWCQKPYRKI
                     TVEDVKYVLSSHYQDTVYDPYGSEGTPVSKKVFRPIGINRTSQTAILHIRPNKPQEIA
                     AIQWMAYGSMPFNTMVPFFTQVKTIPDYFANTYENVSTDNFYWTNRLIAALADPHYNH
                     HETDLDNYLEETMAKGHAMLHAVEAQLLAGETVDLEEENQKMSDYVQGETQTLLNKIL
                     FDASNLMTNRFSLSD"
     gene            39087..40634
                     /locus_tag="VC122_000394"
     CDS             39087..40634
                     /locus_tag="VC122_000394"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010922097.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc ABC transporter substrate-binding protein
                     AdcA"
                     /protein_id="HEP5640348.1"
                     /translation="MKKKILLMMSLISVFFAWQLTQAKQVLAEGKVKVVTTFYPVYEF
                     TKGVIGNDGDVSMLMKAGTEPHDFEPSTKDIKKIQDADVFVYMDDNMETWVSDVKKSL
                     TSKKVTIVKGTGNMLLVAGVGHDHHHEEADKKHEHNKHSEEGHNHAFDPHVWLSPYRS
                     ITVVENIRDSLSKAYPEKAENFKANAATYIEKLKELDKDYTAALSDAKQKSFVTQHAA
                     FGYMALDYGLNQISINGVTPDAEPSAKRIATLSKYVKKYGIKYIYFEENASSKVAKTL
                     AKEAGVKAAVLSPLEGLTEKEMKAGQDYFTVMRKNLETLRLTTDVAGKEILPEKDTTK
                     TVYNGYFKDKEVKDRQLSDWSGSWQSVYPYLQDGTLDQVWDYKAKKSKGKMTAAEYKD
                     YYTTGYKTDVEQIKINGKKKTMTFVRNGEKKTFTYTYAGKEILTYPKGNRGVRFMFEA
                     KEPNAGEFKYVQFSDHAIAPEKAEHFHLYWGGDSQEKLHKELEHWPTYYGSDLSGREI
                     AQEINAH"
     gene            40782..41504
                     /locus_tag="VC122_000395"
     CDS             40782..41504
                     /locus_tag="VC122_000395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003050088.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HEP5640349.1"
                     /translation="MPKEQPLYLQIVDDLEVKIKKSMTENDKLLSERELSDLYGVSRI
                     TIRLALKELELRDLIYKKQGKGTYVSGIKEPVTDLSSTYSFTEQMKKVGKTPKTEILS
                     FEQYQVTPYLSGLLEFDLDTEVFELERLRIADDMPLMFERSYIPAQPFQGLSIADLKR
                     KALYDIFAKDYQETIRLAEEEFYASIALDYEADLLGIKKGDPVLHIIRKTYNDKNILI
                     EFTFSIARADQFRYRVQHHPNG"
     gene            41524..42723
                     /locus_tag="VC122_000396"
     CDS             41524..42723
                     /locus_tag="VC122_000396"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011528441.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SIS domain-containing protein"
                     /protein_id="HEP5640350.1"
                     /translation="MFTKTQEALEALGAAITTKEIKQEPRLWQETMTFFEETRDSLDS
                     FLKRVCKSANGNNVHVIFTGAGTSEYIGNTICPYLKKVGNRQCYLFESVASTDLVAAP
                     DYYLAEEETVLLVSFARSGNSPESVAAVNLVNQLVPNSYHLTITCAKDGELAKKAQQD
                     ERSYLYLMPEDANDAGFAMTGSFTCMMLAALLIFDDANSIAQKEAYVTDMILAGQAVI
                     DQERRLQELADLGFERLVYLGSAGLAKLTQEAQLKMLELTAGQVATLYESSMGFRHGP
                     KSFINDRTLVIGFVNNDAYVRQYDLDMLEEIQADGIALKILALLQGGDINFSEDQFRL
                     DTRHLLPEAYLAFPMILVAQTLALLTAVTLGNSPDTPSATGTVNRVVKGVTIHPYPTS
                     SRQCNKA"
     gene            complement(42820..43080)
                     /locus_tag="VC122_000397"
     CDS             complement(42820..43080)
                     /locus_tag="VC122_000397"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018370011.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type B 50S ribosomal protein L31"
                     /protein_id="HEP5640351.1"
                     /translation="MRKDIHPDYRPVVFLDTTTGYQFLSGSTKASKETVEFEGETYPL
                     IRVEISSDSHPFYTGRQKFTQADGRVDRFNKKYGLKDANAAK"
     gene            complement(43195..44136)
                     /locus_tag="VC122_000398"
     CDS             complement(43195..44136)
                     /locus_tag="VC122_000398"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002985305.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional oligoribonuclease/PAP phosphatase
                     NrnA"
                     /protein_id="HEP5640352.1"
                     /translation="MITTFETILDKIKAHQTIIIHRHQNPDPDALGSQAGLKEIIAQN
                     FPDKKVLMTGFDEPSLTWISQMDQVTDKDYKEALVIITDTANRPRIDDERYTLGQCLI
                     KIDHHPNDDVYGDFYYVDTSASSASEIIADFAFSQNLTLSDKAAKLLYTGIVGDTGRF
                     LYASTTSKTLSIASQLRHFEFDFAAISRQMDSFPLKIAKLQSYVFEHLTIDESGAAYV
                     LVSQETLKHFDVTLAESSAIVCAPGKIDNVQAWAIFVELTDGNYRVRMRSKEKIINGI
                     AKRHGGGGHPLASGANSANLEENQAIFRELIAVCQEI"
     gene            44530..44979
                     /locus_tag="VC122_000399"
     CDS             44530..44979
                     /locus_tag="VC122_000399"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002985303.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flavodoxin"
                     /protein_id="HEP5640353.1"
                     /translation="MALAKIVYASMTGNTEEIADIVANKLQELGHDVDIDECTTVDAS
                     EFENADIAVVATYTYGDGDLPDEIVDFYEDLQDLDLEGKIYGVVGSGDTFYDYFCKSV
                     DDFSDQFALTGAVKGAEPVKVDLAAEDEDIDRLEAFAEQLSQAVNNL"
     gene            45154..45438
                     /locus_tag="VC122_000400"
     CDS             45154..45438
                     /locus_tag="VC122_000400"
                     /EC_number="5.4.99.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002990469.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chorismate mutase"
                     /protein_id="HEP5640354.1"
                     /translation="MRLEEIRQEINGIDHHLVALLEKRMALVEQVTAYKLANHLPVLD
                     QARENQILDRVSYLVKDQAFEPAIHETFKTIMSLSRQYQTQRLTGGDIND"
     gene            45431..46693
                     /locus_tag="VC122_000401"
     CDS             45431..46693
                     /locus_tag="VC122_000401"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002985300.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="voltage-gated chloride channel family protein"
                     /protein_id="HEP5640355.1"
                     /translation="MIKSIATLPKPSLKEALGLIGLALPIGATVGVVDVIFGKGLLFL
                     SEYRDHHLFLLLPFLALAGLVIVFLYDKLGKEVRQGMGLVFQVGHGQKDQIPPILIPL
                     ILFSTWVTHLFGASAGREGVAVQIGATISHYCQRFVTSQEAARHLLIMGMAAGFAGLF
                     QTPIAAVVFALEVLLVGTLRYCALLPSLVAACVASWTSHSLGLEKFTIVLEETLTITP
                     LTLVKLIGLGLIFGLVGNSFAYLLGWFKPYLSQKLPNPYFRIAFIGALLSICLMIGHV
                     GRYSGLGTHLIAAAFSGQTILTYDWLLKMIVTVISLSAGFQGGEVTPLFAIGASLGIV
                     LAPYLGLPVLLVAALGYTTVFGSATNTFWAPIFIGIEVFGPENALAYFVTSAAAYMVS
                     HRHSIYSYQKVVMINLKDMTVNQMDLKK"
     gene            46808..47155
                     /gene="rplS"
                     /locus_tag="VC122_000402"
     CDS             46808..47155
                     /gene="rplS"
                     /locus_tag="VC122_000402"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005590628.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L19"
                     /protein_id="HEP5640356.1"
                     /translation="MNPLIQSLTEGQLRSDIPNFRPGDTVRVHAKVVEGTRERIQIFE
                     GVVISRKGQGISEMYTVRKISGGIGVERTFPIHTPRVDKIEVIRHGKVRRAKLYYLRA
                     LQGKAARIKEIRR"
     gene            47402..47473
                     /locus_tag="VC122_000403"
     tRNA            47402..47473
                     /locus_tag="VC122_000403"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:47435..47437,aa:Arg,seq:cct)
     gene            complement(47516..47773)
                     /locus_tag="VC122_000404"
                     /pseudo
     CDS             complement(47516..47773)
                     /locus_tag="VC122_000404"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017770118.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
     gene            48169..48738
                     /locus_tag="VC122_000405"
     CDS             48169..48738
                     /locus_tag="VC122_000405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002994056.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrolase"
                     /protein_id="HEP5640357.1"
                     /translation="MKGMNYQDYIWDLGGTLLDNYELSTQAFVQTLAFFSLPGDHDAV
                     YQKLKESTAIAVAMFAPNEPEFLHVYRLREADKLAQPIWCLGAKEILEKIATSGSRNF
                     LISHRDCQVNQLLEQAGLLIYFTEVVTASNGFARKPNPESLFYLKEKYDINSGLVIGD
                     RLIDKQAGQAAGFNTLLVDGRKNLLEIVT"
     gene            48739..50691
                     /gene="gyrB"
                     /locus_tag="VC122_000406"
     CDS             48739..50691
                     /gene="gyrB"
                     /locus_tag="VC122_000406"
                     /EC_number="5.6.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002283665.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase (ATP-hydrolyzing) subunit B"
                     /protein_id="HEP5640358.1"
                     /translation="MIEENKHFEKKMQEYDASQIQVLEGLEAVRMRPGMYIGSTAKEG
                     LHHLVWEIVDNSIDEALAGFASHIKVFIEADNSITVVDDGRGIPVDIQAKTGRPAVET
                     VFTVLHAGGKFGGGGYKVSGGLHGVGSSVVNALSTQLDVRVYKNGQIHYQEFKRGAVV
                     ADLEVIGTTDVTGTTVHFTPDPEIFTETTQFDYNVLAKRIQELAFLNRGLKISITDKR
                     SGMEQEEHFHYEGGIGSYVEFLNDKKDVIFETPIYTDGELEGIAVEVAMQYTTSYQET
                     VMSFANNIHTHEGGTHEQGFRAALTRVINDYAKKNKILKENEDNLTGEDVREGLTAVI
                     SVKHPNPQFEGQTKTKLGNSEVVKITNRLFSEAFQRFLLENPQVARKIVEKGILASKA
                     RIAAKRAREVTRKKSGLEISNLPGKLADCSSNDANQNELFIVEGDSAGGSAKSGRNRE
                     FQAILPIRGKILNVEKATMDKILANEEIRSLFTAMGTGFGADFDVSKARYQKLVIMTD
                     ADVDGAHIRTLLLTLIYRFMRPVLEAGYVYIAQPPIYGVKVGSEIKEYIQPGIDQEDQ
                     LKTALEKYSIGRSKPTVQRYKGLGEMDDHQLWETTMDPENRLMARVTVDDAAEADKVF
                     DMLMGDRVEPRRNFIEENAVYSTLDI"
     gene            51059..52783
                     /gene="ezrA"
                     /locus_tag="VC122_000407"
     CDS             51059..52783
                     /gene="ezrA"
                     /locus_tag="VC122_000407"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014407454.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="septation ring formation regulator EzrA"
                     /protein_id="HEP5640359.1"
                     /translation="MSSGIILLIVAIVLLVIIAYLVGVIIRKRNDSLITSLEERKQAL
                     FALPVNDEIEEVKSLHLIGQSQTSFREWNQKWVDLTVNSFADIENHIFEAENLNDTFN
                     FIRAKHEINSVESQLNLVEEDIASIREALNILKEQEEKNSARVTHALDLYEKLQASIS
                     ENEDNFGSTMPEIDKQMKNIETEFSQFVALNSSGDPVEASEVLDRAEEHTIALGQITE
                     QIPAIVAKLEDDFPDQLDDLETGYRRLLEENYHFPEKNIEARFQEIRESIRANSSELV
                     TLDLDRAREENTHIQERIDSLYEVFEREIAAYKVAAKNSKMLPRYLAHVKRNNEQLKD
                     EIARLSRKYILSETESLTVKAFEKDIKEIEDSTLAVAEQFGLQEKPFSELQVTFERSI
                     KTLTNVESGQMDVFAAVKDIEKIESQARHNLDVYVTQLHMIKRYMEKRHLPGIPQDFL
                     SAFFTTSSQLEALMDELSRGRINIEAVSRLSEVATVAIANLEDLTYQVVQNATLTEQL
                     LQYSNRYRSFEAGVQSSFEHALRLFEVENDYQASFDEISYALETVEPGVTDRFVNSYE
                     KTREHIRF"
     gene            complement(52915..53373)
                     /locus_tag="VC122_000408"
     CDS             complement(52915..53373)
                     /locus_tag="VC122_000408"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002990451.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YueI family protein"
                     /protein_id="HEP5640360.1"
                     /translation="MTNLEDKLLKGAWGERRLNPEQQRYYLGTYAERVVLSALLDEAQ
                     TKTVKDYLKRELPSLQLVYQPLHLKISSKLAPQLQILYMKLAKENHLPVTIITETHMT
                     SPFAFILHTDHAINLKETRLEVILKQTKNDQLSKQPPEKTKSFWKRFLKK"
BASE COUNT        16218 a         9474 c        11369 g        16321 t
ORIGIN      
        1 ctatttaaaa aataaagaaa tggcaaaaaa cataaaatac ttgcattttc attgtaattt
       61 gctacaatag taaggtaaag ttagactgta tggcctaccg tctatctata aaatatattt
      121 tattggaggc ttttcctaaa atggcaaaag aaaaatacga tcgtagtaaa ccccacgtta
      181 acattggtac aatcggacac gttgaccatg gtaaaactac tttaacagct gcaatcacaa
      241 ctgtattggc acgtcgcttg ccttcatcag ttaaccaacc aaaagattac gcttctatcg
      301 atgctgctcc agaagaacgc gaacgcggaa tcactatcaa cactgcacac gttgagtacg
      361 aaactgcaac tcgtcactat gcgcacatcg acgctccagg acacgcggac tacgttaaaa
      421 acatgatcac tggtgccgct caaatggacg gagctatcct tgtagttgct tcaacagatg
      481 gaccaatgcc acaaacgcgt gagcacatcc ttctttcacg tcaggttggt gttaaacacc
      541 ttatcgtgtt catgaacaaa gttgaccttg ttgatgacga agagttgctc gaattagttg
      601 agatggaaat tcgtgacctt ctttcagaat acgatttccc aggtgatgac cttccagtta
      661 tccaaggttc agctcttaaa gctcttgaag gcgacactaa atttgaagac atcatcatgg
      721 aattgatgga tactgttgat tcatacattc cagaaccaga acgcgacact gacaaaccat
      781 tgcttcttcc agtcgaagat gtattctcaa tcacaggtcg tggtacagtt gcttcaggac
      841 gtatcgaccg tggtactgtt cgtgtcaacg acgaaatcga aatcgttggt atcaaagaag
      901 aaactaaaaa agctgttgtt actggtgttg aaatgttccg taaacaactt gacgaaggtc
      961 ttgcaggaga caacgtaggt atccttcttc gtggtgttca acgtgacgaa atcgaacgtg
     1021 gtcaagttat tgctaaacca ggttcaatca acccacacac taaattcaaa ggtgaagtat
     1081 atatcctttc taaagacgaa ggtggacgtc acactccatt cttcaacaac taccgtccac
     1141 aattctactt ccgtacaact gacgtaacag gttcaatcga acttccagca ggtacagaaa
     1201 tggttatgcc tggtgataac gtgacaatca acgttgagtt gatccaccca atcgccgtag
     1261 aacaaggtac tactttctca atccgtgaag gtggacgtac tgttggttca ggtatcgttt
     1321 cagaaatcga agcttaattt taattaagtt cccagaatag caatttaagt cagacaactt
     1381 aagtttgaag aagcccccaa ttcgggggct ttttgctatc ttaaactcca taaaatgctg
     1441 ataaataaga atgaaaagct ttcagaaaaa gggtttttat ttgtgaaaat attcctaaaa
     1501 tataagcaat tccttttaat cgtcggtcat ttgtgctaaa atagaagata taaataaaaa
     1561 aagaagaggt atgtgatatg tcacgtaaac caattattgc tggtaactgg aaaatgaaca
     1621 aaaaccctca agaagcaaaa gcattcgttg aggctgtagc aagcaaatta ccttcaactg
     1681 accttgttga tgttgccgta gcagccccag ctgttgattt ggtaaccact attgaagctg
     1741 ctaaagattc agttcttaaa gttgctgcac aaaactgcta ctttgaaaac acaggggcat
     1801 tcactggtga aacatcacca aaagtgcttg ctgaaatggg agctgactat gttgtgattg
     1861 gtcactcaga acgccgtgat tacttccatg aaacagatga agacattaat aaaaaagcca
     1921 aagcaatctt tgctaacggt ttaactccaa ttgtttgttg tggtgagtct cttgaaactt
     1981 atgaagctgg taaagccgtt gagtttgtag gtgcgcaagt ttcagcagca cttgcaggtc
     2041 tttcagcaga gcaagtagct tcacttgttt tagcttatga gccaatctgg gctatcggta
     2101 ctggtaaatc agctactcaa gatgatgctc aaaacatgtg taaagctgtt cgtgacgtgg
     2161 tagcagcaga ctttgggcaa gaagttgctg ataaagttcg cgttcaatac ggtggttctg
     2221 taaaacctga aaacgttaaa gattacatgg cttgtccaga tgttgacggt gcccttgtag
     2281 gtggtgcatc acttgaagct gatagcttcc ttgctttgct tgatttcctt aactaagtca
     2341 tctaaaaaag aaaaggttaa atccttttct tttttagatg acttaactct tactatgaga
     2401 gaataaaggt gattctacag gcaaatgcat tagcctaata gcggtttaat agcttttttg
     2461 ccaatctaat gaaatgatat ttgattggaa atggataaca tctaaatgtc cctggttttt
     2521 gcaaaataaa gccattgaaa ttctgtttaa agcgcaagac accgtctgaa ccatcaaact
     2581 ttccagtaat accaagaaaa ttatattgtt tgatgccctt gtgaattgcc ttgagcatag
     2641 catgctcttg taatagagct gggctataaa atttattaaa tttggggtaa gatcctgaaa
     2701 agaggtagac ggcctcttgt tcggtataga taaaaagact tcccgcaagg aacacatctt
     2761 tagttccgta ctcttcaaga aaatggagag cttcagaaat tcttacctga aaggtttcga
     2821 attggctgga tagttctttt aatctatttt gctttttttc agaatgagga tttttgccaa
     2881 ggtctccttt aactttgttg atgctggttg ctagttgtag ttgtctttgt ttaaggttat
     2941 taaggtaatc ttcaaaatta agagtcgcaa ccataaattc acaagagtct ccaaaactat
     3001 cataaaagtc ctgatagtaa gataatgatt tgtcaaggta atctctacga tcagaggttg
     3061 cttcagtgat ttctttgaaa tggtgtaact catctcgttt cagttgacgt aatttaatac
     3121 caaaagtatt agcttttttt attaaagctt ttcctttttt gctgaaggaa tgggttaaac
     3181 gtgaggaatc aattccttct aactttttaa cgtaatgcca aacaggttca ccctcaggat
     3241 agcctgtttt taaaccgtca tgtttatatc ctaacgccgt taaaaggcta ataaggtcag
     3301 tgtttggatg agaaataggg atgccatcag tatcaaatga ctgataattg tcgtaaggtt
     3361 ttaaaatgag ttcgataacg tcattttgtt tggcatagtc tttaagttga gtgtaaaaat
     3421 gttctaattc ttcgggatga ttggtattag ggcctgcatt gagttccata cgccaacctc
     3481 cagccacttt ttggctaaag accatagcag caacctttag ttcaccatct ttttcaagtc
     3541 caagaaattg tggttttgcc cctcttttag ccatcaagct ggccatttct gaggtttgga
     3601 taaaggaatg atggacgaga ctgtggcagt aatggtcaaa ttgttcttgt gaaatttcaa
     3661 ttagtgccat tagttgtgct ccttaatttt tttcttaagt tataggtcaa gatggctaag
     3721 cttgaaacaa ggccgacagg aatgttgaat tcacctgcaa attcttcgat ggtggggttg
     3781 agtctagctt tgaaatgata gagtccacca tcttgttgat tttccacacc tcctaaatta
     3841 tgccagcggc agcctctttt aaaagcttct ttagccgttt cataccaggt taaaagagga
     3901 gcttgataat tgcgataatc atcgtccatt cccgcataaa gattttcaga agtctcgcca
     3961 taaatcaagg ttagggtggc ggctagtggg atgcgggttt tgcctgcagc gagctgctcg
     4021 cttaaaatgg ttaaatcttt ttgtagccgt gcaatggttt tttgattttc tgctactttg
     4081 ctaggtttac tcttatctgt taatcgtgct tgttctgcta aggccttgtc caactgttgg
     4141 atcaagagtt ttttttgttc aggtaagtct aaactagcca tagtaatgta ggactgacca
     4201 gcataaatat ctagtaattt ttgatagtag gatttgcctc gaaggataat gccttttcga
     4261 ttctcggttt ttttcatcaa ggctgaaaag tcgtctagca attcactgcc accaatcgtg
     4321 acattaacac ctttattggt agcggtacga atggattgct tggctttttt gggcaggctg
     4381 tcaaagtcaa aatccttggc gtagatgttg gcttgtattc ttggttgaat agtgtcttcg
     4441 agttcttttg ttctgccaga ccattcgata ccaagttttt ttagaaaagc aataatagag
     4501 agtgtcacat cattttcttt gctttctttt ccttctaaag tttgtttgat gactaaactt
     4561 ggatcaattt tgacaaaaag cgctcgtttg ctttttccga aagttttgag cgttttaatg
     4621 acgaaatcaa ggagttcaaa attggcgtag tccatgattg gcccacgtgg gatataaatc
     4681 atagtgaaac caagaggaag ttttctgatt aaacaagcag cagcagcaac ttgtacccca
     4741 ttttcataaa agctgatgcg ttcgtgtttc caattatctt tgacttttct ccactttgaa
     4801 ctttgaagta agccagcttg tggctgtgct aagacaaact gatcatgttc ttcaggagat
     4861 attccgattt tatatgtata cattagttta atacccactt gcgaatagca tgtgccacac
     4921 cactgtcatt gttggatttg gtgatgtatt tggctatttt tttcaattca ggaaccccat
     4981 tttccatgac aacagggttg gcaacgactt ctaacatagc acgatcattt tcagcatccc
     5041 cgatggccat ggtttgtgac atatctagcc cgagtttttt agctaagtgt ttgatggcat
     5101 tgcccttgct gacggttttt ggcatgaact ctaaataaaa aggggttgac ttgacgatgg
     5161 tgtacttatc aaagaaatgt tgaggaattt gcttgatggc cgcgtccaaa aggtcaggtt
     5221 catcaatcat catcattttg atgatttctt tattggtcat ttcttcggga gtgcgataga
     5281 aaataggcat gtttaccaaa gtcgattcat gaactgtgta ttttccaata ttccgattgg
     5341 cagtgtaaat accttctttg gtaatggcat gcatgtgaac tcctaatttt cggctcaaaa
     5401 attccgtttc cagataatcg tcgtaagtca tcaattcttt aacaatttct tcgccggtct
     5461 cggcatcttg caccaagcca ccattaaaag tgataacgtg gtttcctttg tggttgagtt
     5521 ctaattgttc gagtaatgaa atcactccgg caataggtct gccagtagca atcaccatat
     5581 gaaccccttg ggctttagcc tcttggacag cttgaaaaac atcatctgta attcgacggt
     5641 catcagtgag taatgtgcca tcaatatcga cagcaaccag tttgatagac atggcatcct
     5701 ccttgttttt ttgtggttta tcttaaaaat gttctccctc ctatcttact aaaaataggt
     5761 gcttttgtca tgggatgaga aaggaaactt aaggaaaaac gagagaaaac gttttagttt
     5821 agctcaatcg cctctgtagt aactaaaaag attgaagaaa gtactctttc ttcaatctta
     5881 gaatggagac aaacccagtt tgatgctcaa aaagcatgaa ctgctctgct gatttgacaa
     5941 ctgatttctt atttctgcgt gtgacgtgag ccaaaacagc tgctttttgc tgtggcaaaa
     6001 gtggtgtcaa tactggcagt gacgttacgc ggaatttttg agaccctagg ctcaaaaata
     6061 aagcatagaa ttccaaagga agtcgctgcc ataagcatca cctaagaaaa gtatcaaaaa
     6121 agactttttc tgcaatctta aagggcttta ctgactaaag tgaaaatgcc cattggaaat
     6181 ataactcata aaagtttcct tgctaggagc aaagaggtca tcgagctcta acatttcctt
     6241 tggaaaataa aagcgacggt ctccgtaggt tgtccctgtt aatgctttga caataggaga
     6301 taactgagat aattcagcaa ggctcccgtc tttttgtatc atttcaatct gagttcttgg
     6361 attttcgagc tctggtcgat aaatatcata aggcaggtcg aagttgatat ggataccggt
     6421 gtaataatct ggatcaaagc caactgattc gaccaattgg cgcaagcgtt ctaactctcc
     6481 tcgggaatct tggtcaaagg taacagactt cagaattttt cgattaataa agcgactggc
     6541 caaatctgat aagatatggt cttcgcttgc catccagact tggaagtaag tattcatgac
     6601 tccgtcatct agggctatat aatctgctaa attagccttc ttttcaaaga aggggatgag
     6661 tccaggagct gttttttgga aataagcctg ttgctcagga tataagtgtt gggctctttt
     6721 gaggagattt tgtaagatga gctcaacagc acggctggcg ggatgaaaat agacctgcat
     6781 gtacatttga aaacgactga caatatagtc ctcgacagca tgcataccac tatgttcaaa
     6841 gacaatgcca tcttcaacag gtcgaatcac acgtagaatg cgcattaaat caaactgacc
     6901 ataattggca gcagaaaaat aagaatctct taaaaggtaa tccatacggt cgcagtcaat
     6961 ctggctggaa atcagttgca caacttgctt gttaggatag gtatgattaa tcacgctggc
     7021 aactttgtct ggaaaatcaa gagcatgacg caccaaaatg gcattgattt ctgtctcagg
     7081 attggtaatt atctcctgag taaaggcctc gtgatccgta tgaaaaagaa cctcaaaggt
     7141 atgagaatag gctccatgac caatgtcatg taagagggca gccgtcatag tgaccagact
     7201 ttcatcttta ttccagatat cagcgtattt ctcttcaaaa atagctgtca cccgacgggc
     7261 aatttcgtaa acacctaggc agtgagaaaa acggctatgt tctgcgccgt ggaaggtgaa
     7321 agctgttgta gggacttgtt tgatgcgtcg taagcgttgg aattctttgg tattgataag
     7381 gtcataaatt aatggattat caatgtggat atagttgtga acagggtcac gaaatacttt
     7441 ttcattcata atttttatta tatcaaaaat tgataaaata gggagaaatt gatagaataa
     7501 acagacgagc ttagaataga aagaagagtt atgaaattac agttaaccaa ccatattcgc
     7561 tttggagatg agacagagat tattcaagaa attcatgatt gtgagtggcg agaaaaaggg
     7621 ggctatcagt acctcatcta tcaaaacact gacaaggaaa aggttgtcat caagtataat
     7681 gagacagaac tgaccatgag ccgtttttcc aaccctcagt caatcatgaa attttttgcg
     7741 ggcaagaagg tcctgattgc tctacctaca ccgatggggg ttcaacaatt tttgacagat
     7801 accagtcatt accacttaga ttgttcttgt caaaaattgg accttcatta tcatttgctt
     7861 caagctcaaa cagaaatgct atttgcaagc tatcatcttg agctgagttg ggattaacta
     7921 aaaagatagc agtaaatcat ctgattaggg attattcttg ttttttagtc aggtttttac
     7981 tataatggtt attatatcaa gggagtagca gacggctagc cgtgattgta tcgtcaatac
     8041 ggaatttcgg tttccggtac aatcttaaac agcgagactt gtttaagaaa ataaacaggt
     8101 ctttttattt gaacaactaa aataaaaaga ccaaggaggt actaatttgt ctaaagaaca
     8161 aagacatgaa gcattttaca cgcaaagtga agagaccgta ctggctcaat tagagaccag
     8221 tcgcgaaggt cttacgtctg cccaagcaaa agagcgctta gcagagtatg gtcgtaatga
     8281 gcttgatgaa ggcgaaaaac gaagtttatt tatgaaattc cttgatcaat ttaaggattt
     8341 gatgattatt attttgattg tggcagccct tctttcagtt cttacagagg ggatggaagg
     8401 tttgacagac gccattatta tcttggctgt tgttatccta aatgctgttt ttggtgttta
     8461 ccaagaagga caagctgaag ctgctattga agccttgaaa tcaatgtcaa gtccactggc
     8521 tcgtattcgc cgtgatgggc atgtgactga gatagactct aaagaattgg tccctggaga
     8581 tattgtcttg ctagaagcgg gagatgttgt ccctgctgat ctgcgtcttt tggaagctaa
     8641 ctccttaaaa atcgaagaag cagcgcttac tggagaatct gtaccggttg aaaaagactt
     8701 gtcaacggct gtttcagaag atgccggtat tggtgatcgc gtcaatatgg gatatcaaaa
     8761 ctcaaatgtg acatatggtc gtgggattgg ggttatcact aacactggca tgtacaccga
     8821 ggttgggcat attgctggta tgcttgctaa tgctgatgag acagatacac cattgaagca
     8881 aaaccttgac aacctttcta agattttgac ttatgcgatc cttgtgattg cagcggtgac
     8941 ttttgctgtt ggagtcttct tgcgtggtca acacccgctt gaaggcttga tgacttcagt
     9001 agcgcttgcc gttgcagcta ttccagaggg acttcctgcg attgttactg ttgtgctttc
     9061 tcttggaact caagtacttg ctaaacgtaa cgccattatc cgtaaattac cagctgttga
     9121 aacgctagga tcaactgaaa tcattgcttc tgataaaacg ggtaccttga caatgaatca
     9181 aatgaccgtt gaaaaagttt ataccaacgg gacactccaa agttcatcag ctgatattgc
     9241 ttttgacaat acgacacttc gtgtaatgaa ctttgctaat gatactaaag ttgatccttc
     9301 agggaagtta attggtgacc caacagaaac agctttggta cagtttgggt tagaccacaa
     9361 ttttgacgta cgtgaggcta tggtggcaga acctcgtgtg gcagaattac catttgattc
     9421 tgaccgtaag ttgatgtcaa ccattcataa acaagctgat ggcaaatatt tcatcgctgt
     9481 taaaggtgcg ccagaccaat tgttgaagcg tgtcacacag attgaagaaa atggacaaat
     9541 tcgtcctatt acggatgctg ataaaaaaac cattcttgat accaataaga gtcttgccaa
     9601 acaagctctt cgtgtcttga tgatggctta caaatattct gatgctcttc caacactgga
     9661 aacagaaatt gttgaggcta atcttgtctt ctctggtttg gtagggatga ttgaccctga
     9721 acgtcctgaa gcagcacagg ctgttaaagt agctaaggaa gctggcattc gtccaatcat
     9781 gattactggt gaccaccaag atacagcaaa agctattgcg aaacgtcttg gtattatcga
     9841 agaagatggc gttgatcatg tctttacagg tgctgaactc aacgaattat cagatgaaga
     9901 gttccaaaaa gtctttaaac aatactccgt gtatgcccgt gtatctcctg agcataaagt
     9961 tcgtatcgtt aaggcttggc aaaatgaagg caaagttgtt gccatgactg gcgatggggt
    10021 taacgatgca ccatctctta aaacggcaga tattggtatt ggtatgggaa ttacaggtac
    10081 agaggtttct aagggagctt ctgacatggt tcttgccgat gataactttg caaccattat
    10141 cgttgcggtt gaagaaggac gtaaggtctt ctcaaatatt caaaaaacaa ttcaatattt
    10201 actttctgct aacatggcag aagtctttac catcttcctt gcaaccttgt ttggttggga
    10261 tgtgcttcaa ccagttcact tgctttggat taacttggta actgataccc taccagctat
    10321 tgctcttggt gttgaacctg ctgagcctgg tgtgatgaaa cacaaacctc gtggacgtaa
    10381 gtcaagcttc tttgatggtg gggtgaagga agccattctt taccaaggtg ccttccaaac
    10441 cattttggtg cttggggttt atggttttgc cttgatgttc ccagaacaca caagctacca
    10501 tgatgtccat gcagatgcct tgaccatggc ttatgtgacc ctcggtttaa ttcagttggt
    10561 acatgcctac aacgtgaaat ctgtttacca atccatcttt actgttgggt tgttcaaaaa
    10621 caaattgttc aactattcta ttccagtggc ctttgtagca ttgatggcaa cagttgttgt
    10681 tcctggattt aatcaattct tccatgtgac acacttgacg atcacacaat ggttagtggt
    10741 tattatcgga agtctcttga tggttgttct tgttgaattg gtgaaagctg ttcaacgatc
    10801 acttggtcaa gatgaaaaag caatttaata gacgctaaaa ggtttgagac aattgtctca
    10861 aacctttttt tataatcgtt acaacgataa ggactattgg tatgcctgac attaaaaagt
    10921 tgattcttgc caattgactt gacagtcaaa aatttgttgt ttggcttctt gactgtgacc
    10981 ttcaatatca tcgattaaaa tcttagtagc ctgttcgcct tcttcatagg cgggttggat
    11041 aatagttgtt agtgttggtg aagaaaattt tgtccactca atgttatcaa atcctactag
    11101 tccaatctct ggtatattaa atttaagaga cttcatagct gtaaatactt ttggaagagc
    11161 ccaacaattg gggacaaaaa ccaaactctt tttagtcagt gatccttgca aaaactgagc
    11221 gattgcctct gaagaagtct gattttcatc aataatcatt tcttgatgag taagatgatt
    11281 agcttctaac acatcaatga agcctgaagc gcgttcaata cgagtgctaa gtaaattggg
    11341 gttacccgtg atcatgataa aatgttcata acctttatcg atacattgtt gaatggtatc
    11401 ataaacagca tcataattat tagttttgac ccagtttgtg cgatgttcgt aaagttggct
    11461 atcaaaaaag accacttttt tcttcttaat atcgataatg cgagaatact tcctgaaatt
    11521 agaggtaggc tgaatgataa agccatccac tccgaggttc agcatctttt caatgagttc
    11581 atcttcgcga gaagggtcgt agttgctgtt tccgatgata atttggtaac caaattcctg
    11641 ggctttgctt tcaattccct taacaatttg attggaaaaa ctattggtaa tatcaccaat
    11701 aaccacgcca atgagtttag tagatttggc gtttagactt ctggcagcaa tgcttggttt
    11761 ataattggta gcttcaatcg tgcttttaat gcggttttta gtttcttcag acatcttgtc
    11821 aaacctacca tttaagtaga aagaaactgt tgtttttgaa gttttcgcta attcagcaat
    11881 atctttgatg gttactttgc gctgcattgc atacactcct ttaaaatagt aatggttagt
    11941 catattataa cataaggact cgtaaaagat ttagtgtttc aatcgaataa tctcattcgt
    12001 gatatggttg ataacaacca cctttcctct gattttatga ccatttagga gaactagttt
    12061 ttctccttta attaagtcct cttggagaac gcagatactg tagtcaaact gctttccttt
    12121 taaatgccag gaaagggcgg tctcaattgg gttgcgtttg cctgtttgaa gtggtgttaa
    12181 gggagtaact ttggtatcgt cagggactaa cagggtactg gtgcaaccct tgtctttaaa
    12241 gggttttcgt ttgatgaatt tgtgactagt caggtattgg ttgtattttt ttgacaaaat
    12301 cgtttcttcg atagtgaaag ggcagtcact gattagtctg aggtcactaa ggtgatctgt
    12361 ctttacttct aagtgattat caagaataaa ttgactgaca agacagtggt taccttgagc
    12421 ctgtatcgta tcaatgataa gaaacattcc tggtggtaag atgagaattt gtcggtcatg
    12481 ttggtagttg ttcctatcag agaattgcgt ctggtagcta ccttcaataa agtaaacgtg
    12541 tccattttgt cgaaggtgac aataattgga tttggggtaa gaaaggtaag cccaggagct
    12601 attaatctgc tcaggtaatt gttcttctaa gaaggcggta gaatgatggc tagcgctttt
    12661 tagggcatag cgtaatggtt cttctttgta agtgtatcgc ccagcatcac agaaaagtgg
    12721 ttgtccttta taatagagac agatactatt ttggtcgcta tgggtatggg aactaccgat
    12781 gggcccattt ttgaaaaaaa gataataatt ttcttgattg atggtaatgt gcccggaatg
    12841 ttcaaagtga tgcgcaaaag ttggtaaggt ttggacgggt aactgttcaa aagtgtggac
    12901 acctttttct cctagaagga gaagactatc gatatcaagt gtgggaaaag cagcagcttt
    12961 taagtgtggt tcttcaagga tagtggctgc tagtgttaga atatctctgg tatcggtaaa
    13021 gtcactgtct cctaagggga tctgtttgtg gtcagggcca gtcattttga ggagataatc
    13081 actcatggca agcaaagtag gtctaagctg tggaagataa tcagggacta gagcagttaa
    13141 ttccagaagg gctttgagta cttccacatg atacatggtt gattgttcaa attgactgcc
    13201 gtcttcgaga atttggagtg cgatttgttg cgtcaattct tttctagcga aagctgttgc
    13261 tgcagcaata tcaaggtcag aaccaaagta ggcatcagct aataaaatcg cagtggtttg
    13321 caagattccc caattgctga gactgtattt atcaaggtag ttggcatgga gaaagtgaag
    13381 ttgtttttcc agggaagcta ggatgagcct ttcctcttgc ttggttaagg cgtcaaaaag
    13441 attaaggtaa atcaaacatt tgacccaagc aaaacaacgg atgccagtat ctaaggttct
    13501 agtagctaac cccttaggat ctaaaggaat agctgattca atccaattga gaatgaaacc
    13561 tttagctgtt aagagatacc gttcatctct ttcaacaagg tagacgagga ttaatgtttg
    13621 gagataggtc tgtcgattta acataaaatt ccactcagga tcatctgtga cagcctcttg
    13681 ccaagtaatg ggatctaagt ggtaagggat gtggcagggc tccatatccc aattatcttc
    13741 aaataaaaag gtattggtta agagtaggtc agctatcctt tttcgatcag catagctgtc
    13801 agcctgatag tctagcaggt aattcctgca aaagtttgga ttgactgttt ctttaaagcg
    13861 tgtgaaggca agagacatag gacgtttcct ttccaaaaga aaagagacct gttaatgtga
    13921 ctaggaaata gttcctaaac ccataacaga atctcagtga caactaagct aaaataccca
    13981 accaactgcc aataacacca atgataacag ttaaaatgac caatttgtag gttgtccatt
    14041 ttttgttttt aataaggtaa tacatgagta aggtaaagag agctggtaaa agtgcagggg
    14101 caatcttatc aagcattcct tgaatagtca caaatttttg tgctgtattg gccgcatcca
    14161 ccttgccagc cgcaaaagta atgggaacag ttattttaac agaagtagca gctaatccag
    14221 cgataacggt aacaccgaca atatttgcca tacgagatac tatcgccatt tgttcgctta
    14281 gtttatcaat aaagcttgtg ccgaccttgt agccatagag accagaaccg attttaatag
    14341 cagttaggat aatattcatg gctaggaaga agaaaattgg acctaacact aaaccatcac
    14401 tagctaagga agctgcaatc gttgaaaaga gtggcgccaa gcaaaattgt gataaggagt
    14461 caccgatacc agctaaaggc cccataagtg ccattttgat gtttcgggtt tcttcttcag
    14521 gacgatcatt ttctaacatg accaagtgca gactggtgat gaaaggaagg aagtgagggt
    14581 tagtattgta aaactcacag ttatcttcta aagcttgata taagcccttt ttatcactgc
    14641 caaaatgttt tttcaaggca ggatagatga cattggcgta tccgatacct tggtaattac
    14701 tgtaattaaa cccattttgt aggaagaaag ctctcagagc tgttttgagg taatctttct
    14761 tggttaattt attagatcca gtcatcttgt tggcctcctt ggacagttgt tgcatcgact
    14821 tgtttaggtt tgttgtaaaa ttcattcaag gcgaaaatga taccaataat ggcgatacca
    14881 atggttggaa tttggaggta ggctgcacaa acataaccaa tcagtacaaa aggaatcaac
    14941 tccttcttgg ccataactga taagatcatg gcaaatccga tagcaggtaa cattttacca
    15001 gcaacagtca atccattgag caagacaggt gggatataat caaccaagtg aaggagggta
    15061 tccattgaca aggcacctaa taaaccaagg cctaatccca taaaagcgaa agcccagata
    15121 gtgccgttgg cagcgaattt gaagcctcta atattgcctt tttgcaattg ttttttagcg
    15181 gtttcaggag ccccagcaaa agcggtatag gcgaatgtct gaagaaattg gatgacaact
    15241 gcgattggtg ttgataaggc tagggccgct tctggggtaa ctttaccagc actggtaata
    15301 gccatcaagg taccaaagat accagggccg ataggattag gaggaacggt accaccggca
    15361 cccactccaa atcccatata agctaattcg gagatagctc ccatgctaag tgcagttggc
    15421 aagtcaccta agatcatccc tacaccaaaa gacagaataa tacaacggtt ggtgtagatg
    15481 ccaagtaaca ttccgctgaa acaaaaggct gtccaaagac caattaaaag tgcctgtaac
    15541 aaattgatat ccatatcaat ctcctttatt cattaaatat aatctaagat gtcaacgtca
    15601 gaggcgctat tgccagctgg agttgttttg gtgttaaaaa ccacgtggtg gtcatgggct
    15661 aagcaacgaa tagcagactt gtctgtttct cccagcgaaa taaactgggt aatcgcaacc
    15721 ttatcatcag ttttatgaat attaccgata ttgatttctg tgatgggaac acccccttca
    15781 actaggcgtt tggcatcttg caaatctttg accacgatga agatactttg agcaggactt
    15841 gctttatgaa tgatgtcaat cactttttga atggagaaaa aacgaatggc aatactggat
    15901 ggaataactg ttttcatgag ggattgttga atcttatctt cagaaaccgc atcgttagcg
    15961 acgataacgg tgttgcagtt gaggaatttg acccataatt gcccctgtcc gtgaatcaag
    16021 cgctcatcaa ctcgcgtcat gataatgttt ggttgtgtca taaaatctcc tttttaaatg
    16081 tcggtatcgg tgtttgttac cagtaagggt tccagtcttt gtagaaacga agcagagctt
    16141 caagatagta atagtctccc cagatatttc cctcgtcaac cccttttcca gagtgccaag
    16201 aatagactcc gtgaagtagt agaggagcgc caggattgag atttttgtta gcataatcct
    16261 taatcaaggc tcttagcatg ctgtgcatgg cagcttcata tgttttttta tcaggatcat
    16321 gatctggtaa ggtttttaac atttcatgaa taccacatac agcaatggcg gttgctgaac
    16381 tatcacgaga ctgctcgcta ccatctccaa aaattaaatc ccagtaggaa acctggtctt
    16441 taggaagacg gttcatgaaa taatgggtca tgcccttgaa gagttgaatg agttcaggtt
    16501 cttttaggaa acgataggtc aaaggaatac cataaatgcc ccaagcttgc cctctggccc
    16561 aagcagaatc atcgctataa ccttgtcggg tgaccccttt aataggatcg cctgtttctg
    16621 gatcaaagta gaaggtgtgg taagcagagg cgtcatctcg gatgacatga ttggctgagg
    16681 cgtagaaatg attgatcgcc atgtcacggt aacggttgtc tccagtttct tggctagcaa
    16741 aaaagagtaa ttggatgttt aggagacaat caatgattaa acggtaatct tcttttttac
    16801 ccagcacccc ccaggcctga ataaagccgc ctttgtcttg atagcgttgg attaatttgt
    16861 cggctgcttt caaagcagct tctctagact caggtgtttt taaaagtttc cactcagcca
    16921 tgcaagaagg ggtgtaaaga aagcccaaat catggtgatc tagctcaata tctcttgtta
    16981 ctcgatctag gaaagaaagg tcatttgctt gagccagagc tttaatggca tcatccccac
    17041 cgtattcgta agctaaccaa aggcaacctg tccaaaaggc attggtccat tccgtgttgt
    17101 ccataatggc gtattggtta tctttggtag ctggcgttgg aaagtcttct ttaaaatagt
    17161 ccatattaag ccgaacttgt ttgagggcta agtcaagggc ctgagtaatg tcttcttggc
    17221 ttagaaagtc ttctttggta aatcgttcag gctgcttgat aggttcaaga gcaatggttt
    17281 ttaaagatcg tgtcattaaa tgcctcctaa ctcatcgtct gtcgtaccgt cttcttgctg
    17341 agaaagagtt ttccagtcaa caatgccttc tttagaacat gttataagtc ctgatactaa
    17401 gtcgtcaaaa gaagctgctg tttgtcttgc aaaactagct tcaatcagca tggctaagtt
    17461 aaggccagaa agaacattac acgtcgtgtt tggaagagat tccattaagg ttgcagcgac
    17521 tttaaaaggg gtacctccta gaaggtcaca aagaactaag gtttcctctt ctgggattaa
    17581 agcacgagat aattggtcct gcacatcagc ggcggtcatc tcagtagtaa aatcaattgc
    17641 agttaccttc tcttgtggtc cggcaattaa ctctagggat gagacaatcc cactcgcaaa
    17701 atgtccatgt cccataacga taatagcaat cataaataaa tctcctttgg tttaccggtt
    17761 tactcaaaaa tattgtagcg ctttcaaaat tctttgtcaa tacttttttt aaaagatttt
    17821 tgaaaaagaa aaagtgaacg gttgtgctaa aaaagaatca aaaatggtct tttttgtcac
    17881 tatttaatgt agaaaaatct taatagctat taagtatagg acaagactaa acaagaatga
    17941 cagtttggat aagagcactc ttttaaattt tgaaccgttt tcattaaaat gattgacaaa
    18001 actagtttac tttgttatag tcttggtaaa caggtttact taatctaata acaaaaagga
    18061 gaatcctatg gaaaatatgt tttcgttaca aggtaagatt gccttgatta ccggagcatc
    18121 ttacggtatt ggttttgaaa ttgccaaagc ctatgctcaa gcgggtgcaa ctattgtgtt
    18181 taatgacatc aaacaagagt tagttgataa gggggtggct gcctatcgag agttagggat
    18241 agaagcgcat ggctatgttt gtgacgtgac tgatgaagct ggtatccaac agatggttag
    18301 tcaaattgaa gatgaagtag gtgttattga tattctggtc aacaatgcag gtattattag
    18361 gcgtacgccg atgcttgaaa tggccgcaga agattttcgt caagttattg atattgattt
    18421 aaatgctcct tttattgtgt caaaagctgt attaccatca atgattgcaa agggacatgg
    18481 taaaattata aatatctgtt ctatgatgag cgaattgggg cgtgaaacag ttagtgccta
    18541 cgcggcggct aaaggtggct tgaaaatgtt aaccaaaaat atagcctctg agtttggaga
    18601 agccaacatt caatgtaatg ggattggtcc tggctacatt gcgacgcccc aaacagctcc
    18661 gttgagagag cgacaagcag atggtagtcg tcatcctttt gatcaattta tcattgctaa
    18721 aacaccagca gcacgttggg ggacaacaga agacttagct ggtccagctg tttttctagc
    18781 tagcgatgcc agtaattttg tcaacggcca tattctttat gtcgacggtg gcattttagc
    18841 ttatattgga aaacaacctt aatcacacac tagaaagaga ttttatatga aaattgcatt
    18901 aattaatgaa aatagccaag cggctaaaaa tggtattatt tacgatgccc ttacctcagt
    18961 gacagataaa cacggctacc aagtctttaa ctatggcatg tatggcacag aaggtgaaag
    19021 ccagttgacc tatgttcaaa atggactctt agcttctatt ttattgacca ctaaggcggc
    19081 cgattttgtg gtgactggtt gtgggacagg tgtgggagct atgttggcct tgaacagctt
    19141 cccaggggtt acctgtggtt ttgcgagtga gccaacagaa gcatatctct tttctcaaat
    19201 caatggtggt aatgccttgt ccattccttt tgccaaaggt tttggttggg gagcagagtt
    19261 gaatttgacc ttaatatttg aacgcctctt tgcagaacca atgggtggcg gttaccctaa
    19321 agagcgagct attcctgaac agcggaatgc acgtatttta agtgatctga aaaaaattac
    19381 ttacagagat ttgttggcaa ttgtcaaaga catcgaccaa gatttcctca aagaaacgat
    19441 ttctggcgct cattttaaag agtacttctt tgccaatgcg gagccgtcag aattagtcgc
    19501 ttatttgaaa tcagtacttg aacagtaatt ccattttgac gaggaaagga ggtcacatgt
    19561 ctaagttatt attggttggt gaaccgctga ttcgcgttag ccccaatcag tttcagcctt
    19621 tgaccaatgc ctgcgacgct cagctttttt tcggcggttc agaagctaat attgcaagaa
    19681 ctctaggcgg ttttggctta gaagcaaggc tatttacggc cctaccagat aatcctgttg
    19741 gtcacgcttt tcatcagttt ttgaagcaaa gcggtgtgga catgtccttg acggcttggc
    19801 aaggtcatcg cgttgggcta tattaccttg aaaatggctt tggctgtcgg gctagtcagg
    19861 tttattatga ccgctgtggc tctagctttt cggccttaga taaggatagt cttgatttag
    19921 ctgccatttt tgaaggcatt agtcactttc attttagtgg gatcagtttg gcgctgggca
    19981 aaaaaactca ggatctgatt gagatactag cgagagaagc taggaaaagg caagtttgtg
    20041 tttcttttga tcttaacttt agatctagta tgattgcggt agctgatgct aaacgactct
    20101 tttctcactt tgctcaatac gcggatatta tttttggcat ggaacctttg ctgttagata
    20161 gtgacgattt tgacatgttt gataggaata aggcagatac tacgatgatt agagaacgtc
    20221 tggcaggact ttatcagcgc tatcagttac aagctattta ccatacggag cgttcaaatg
    20281 atgcccaagg cagtaatcgt ttcaaggctt atgcctatga tgggcagttt tacgaatcct
    20341 gtgaagtgac gaccccagtc ttgcaacggg ttggaagtgg agatgctttt gtggcgggtt
    20401 tactttatca gttgttagaa ggtaacgaaa agcagcgcaa ccttgatttt gctgtggcaa
    20461 cagctagcct aaaatgtaca gtagctgaag atcagctcta tcacagcgtc caacaagtag
    20521 aagcagtctt acataatcaa cgggatgtcc agaggtgatg atatgggaaa aatagaaatc
    20581 ttaaccaaac ttaaggctaa tcggcttgtc ctggttgtac gaggagagtc tagtgaggaa
    20641 gctttagctt gcagcctggc cagtattgaa ggtggcatta agacaattga agtcacctat
    20701 actaacccct ttgctagtga ggttatcggt caattagctg aacgcttcaa agaagatcct
    20761 gaggtattga taggtgctgg gacagttttg gatgatgtca ctgcccgcca agctatttta
    20821 gcaggagcac agtttattgt tggtcctaat ttcaatcgag cagtggcttt gatttgtcat
    20881 ggttatagca taccttattt accaggttgt atgacggtta atgaagtggt tacagcatta
    20941 gaatcagggg ttgatatggt caaaattttt cctggttcta ctgtagggat atcctttatt
    21001 cgggcaatca aatctccttt gccacaagta gaggttatgg ttacaggcgg agtttcttca
    21061 gataatctca aagactggct agctgctgga gtagatgttc taggcatagg aggcgagttt
    21121 aatcagttag cgagccaaaa acaatataac ctaatcacta agaaagccgc tcattatatc
    21181 aaaagtttgc gacaataatc taaaaaacgt ttttaccttg gctttagtgc tagtaaaaac
    21241 gtttttttat cattcattca attaatatgc ttctgctcat gtttttccag atagtataga
    21301 tataagagca gctaatttaa caagatgagc ctgttgtttg atatatagga tataaacttt
    21361 ttatgatcaa acaaaagtaa taccttttga attcgttagt attgatctga tctaaagtga
    21421 catcacttag aatggactag ctgtacagct tttacagcaa cttatgctaa aatagaaaaa
    21481 aggagaaatg ttgatgatta aaggaattat ttttgatatg gacggtgttt tatttgatac
    21541 agaacctttt tatctgaggc gacgagaaga tttttttaag acaaagggaa ttcccattga
    21601 tcatttgaac tctaaagatt ttattggggg caatcttcaa gaattatgga aagagttgtt
    21661 aggtaaaaat agggatgatg ctatcgttaa ggcaattaca actgactatg acgcttacaa
    21721 acaagcgcat aagcctcctt atcaaaaact gttgattaca gaagtgaact cttgtcttga
    21781 acagttgaaa aaacaaggta ttaaactggc tgtggcatca aactcgaagc gtcaggatgt
    21841 tttgttggcg ttggagacaa cgcaaataaa agattatttt gaaataatcc tagcgcgtga
    21901 agatgtttct agaggcaaac cttatccaga tatctataat aaagcagtac aaaaactagg
    21961 attacaaaaa aaacaactgc ttgtagtaga agacagcgaa aaaggcattg ctgccgccaa
    22021 agcggccaat ctgacagttt ttgccattac cgactaccga tatggcattg atcagagtca
    22081 agctgatcac aagatagatc atttaggaca actgtgtgta aaaatcggtt gttttggatc
    22141 gtgaagtaac gcaactaact gatcattgat gatacttcaa gacacttaag aatacgtcta
    22201 ggactttcta cagaccaata acaagaatac gtttatgtgt ttttactgtt ttttagctac
    22261 ctatttacaa tatgacactg aaaagagtac tatgtttaag ttattatcaa aaaaaaccgt
    22321 cgcataagat cacctagaaa aggattatga tttttgtgat ttggatgcga tcattagtcg
    22381 ttgaccatct ttatgtggca ggagatatgt ttaaccgtgg tcattatttg gatttgtttt
    22441 tagattggct agtgagtaaa tatagaagga tcataatact gctttgacaa aggcagtatc
    22501 tgagtccatg agttgcacgg gtaatgtgac tcggttttgg ttaggtataa taatatttgg
    22561 aagtgttatg ctgcttgtct tcgtaggtcc attgcttata gtcagactta ctttagagct
    22621 gagctgactg tctagacaag acgtgatgga gatgtgattt ctgaccaagt agaggtaacc
    22681 agaaaaggga cttttacgat gtaattgaat ttacttgttg cattcttgtg catgttaaga
    22741 gagaaaaccc agataaatca ggaagtaaaa tggttatgtt tgattagaat tgtccttctt
    22801 tttccttgaa gtcttaactt attttgatta tgatagtagt atgactatta aggcagaaat
    22861 taaggcactc gccaaagaaa ttggtatctc taaaattggt tttacgacgg cagataactt
    22921 tgattatttg gaaaagtcat tgcgtgcttc tgtggaagag gggcgcaatt caggatttga
    22981 acataaagtc atggaggatc gtatctaccc tgaaaggctt ttagaatcgg ctaaaacgat
    23041 tatttcaatt ggcgttgctt atcctcacaa acttcctcag caacctcaaa aaacacctta
    23101 taaacgtgga aaaattacac ctagttcgtg ggggcttgat tatcattatg tggtcggcga
    23161 aaagttagac agactatcaa aaggcattga agagctgtgt cgagattttc ctttgcaaca
    23221 aaaagcaatg gtcgatactg gagctttagt tgatactgca gttgcccaaa gagcgggtat
    23281 tggttttata ggaaaaaatg gtttagttat ttctaaagaa tatggctctt atatgttttt
    23341 gggagaatta attaccaatt tagagatcga gccagacaag cctgtcgatt atgattgtgg
    23401 tgactgtcgt cgttgtctag atgcctgtcc aacttcttgt ttaattggtg atggctcaat
    23461 gaatgccaag cgttgtctat catttcaaac ccaagataag ggaatgatgg acatagagtt
    23521 tcgaaaaaag attaaaacag ttatctatgg ttgtgatatt tgccaaattt gctgtcctta
    23581 taacaagggt ataaacaatt caccagctac agagattgat cctgagttgg ctcaaccaga
    23641 attaatacca tttttgagtt tatcaaatgg acaatttaaa gaaaaatttg gtatgattgc
    23701 tggaggttgg cgaggtaaaa atattttaca gcgtaatgcc attattgcgc tagcaaatgc
    23761 tcatgataaa accgcagtgg tcaaattaat agagattatt gataaaaata ataaccctat
    23821 tcatactgca acagctatat gggcattggg agaaattgtc aaaaaaccaa acgatgagat
    23881 gctggcgttt atgagtcatt taaccttaaa agatgaagat agccaaaaag agttggaatt
    23941 aatccgtcgt aaatggcaag tttaagcctt ttatggtatt tttaagaacc ttatgataaa
    24001 ataaaacaga ttaacaaaga ttggagaaat ctcatggaag tggctgaaat tcgccaaaaa
    24061 atagtagaaa ataaagagaa gttgactagc ttcaggaggt ctctttgact tagatcgttt
    24121 ggaagaggag attgctcttc ttgaaaatca catgactgag ccagactttt ggaatgacaa
    24181 tattgcagcg caaaagacat ctcaagaact aaatgagtta aaaggtaagt atgatacttt
    24241 ccataatatg caagaattat ctgacgaaac agaactctta cttgagatgc ttgacgaaga
    24301 tgattctttg aaagaagaat tagaagaaaa tctaatgcag ctagataaga taatgggtgc
    24361 ttatgaaatg acgcttctct tatctgaacc ctatgaccac aataatgcta tcttagaaat
    24421 ccatcctggt tcaggaggaa ctgaagcgca agattgggga gatttactct tacgaatgta
    24481 tacccgtttt ggcaatgcta atggtttcaa agtagaagta ttggattatc aagcaggaga
    24541 tgaagctggt attaaatcgg ttaccctctc ttttgaaggc cccaatgctt atggtttgtt
    24601 gaaatcagaa atgggagttc atcgcctcgt tcgtatatca ccttttgatt cagctaaacg
    24661 tcgtcatact tcatttgctt cagttgaagt gatgccagag ttagatgata cgattgaagt
    24721 agaagtacgt gatgatgaca ttaaaatgga tactttccgt tccggaggag ctggtggtca
    24781 gaacgtcaat aaagtatcaa caggtgttcg tttgacgcat attccaactg gaattgtggt
    24841 atcgtctacc gttgaccgta ctcaatatgg gaaccgcgat cgcgctatga agatgttgca
    24901 ggctaagcta tatcagttag aacaagaaaa gaaagcacaa gaagtagatg ctctcaaggg
    24961 tgataaaaaa gaaatcactt ggggaagtca gattcgttct tatgttttca ccccttatac
    25021 gatggtgaag gatcatcgta ctaactttga gcttgctcag gtagataagg ttatggatgg
    25081 agaaatcaat ggctttattg atgcctacct taagtggcgc attgaagact aaaaataata
    25141 aaggagaaac atggcactaa tagaaatgtc tggtgttact aaaaaatacc gccgctcaac
    25201 gaccgccctc agagacgtca atgtttctgt taatcaaggt gagtttgttt atctcgtagg
    25261 cccatcaggt gctggtaaat caacttttat caaactcctt taccgtgaag aacaactgac
    25321 aactggtaag ctatatgtgg gtgaattcaa tttaactaaa ctaaaagcac gtgatgttcc
    25381 gattcttcgt cgtcacatcg gagtagtttt tcaagattac aaactgctac caagaaaaac
    25441 agtctttgaa aatgtagctt acgccatgga agttattggt gaaaagcgcc gccacattaa
    25501 aaaacgcgtg ccagaggtac tcgatttagt tggcttgaaa cataagatgc gttcttttcc
    25561 aagtcagcta tcaggagggg agcagcaacg tgtggccatt gcgcgtgcga ttgttaacaa
    25621 ccctaaatta ttgattgcag atgaaccgac agggaattta gatcctgaga tttcatggga
    25681 aattatgcag ttacttgaac gtattaatgt tcagggaaca actatcttaa tggcgacaca
    25741 caacagtcat attgtgaata catttcgtca tcgtgttgtt gctattgaag atggtcgcat
    25801 agtacgtgat gaagaaaaag gagattatgg ttacgatgat tagatacttt ttccgtcata
    25861 tttgggaatc cattaaaaat cttaaacgca acttttggat gacatttgct tcagttagta
    25921 tggtggctgt tacattgact ttagttggtg tttttgcggc gacattgttg aatatacagc
    25981 gtgttgcttc gggtgttgaa aataatgtcc atatcaatac ctacttacaa gttgattcaa
    26041 ctgatgctgc aaaagttatt caaaatactg ccggtgaacc tgttaataac gataactacc
    26101 acagtgttta cgacaaaatt gctcagataa aaggtgtcaa aaagattaca ttttctagca
    26161 aggatgaaca attgaaaaaa ttgcaagaaa ccttaggtga tgtttggaat atgtatgacc
    26221 aagacactaa tccgctccaa gatatctatc ttattgagac ccagacgcct aaacaagtta
    26281 aggcgatcac taaaaagatc agaacgattg aaggcgtaga agccgcagat tatggaggga
    26341 ttaactccga taaactattt aagttttcaa cattaattca aacttggggg ttgatcggaa
    26401 cagcaatgct attatttgta gctgtctttc tcatttcaaa cactattcgt atgactatta
    26461 tgtctcgtaa acgcgacatt gaaattatgc ggttagttgg tgctaaaaat tcttatattc
    26521 gagggccttt cttttttgaa ggggcttggg ttggcttgtt aggagctgtt ttaccatctc
    26581 ttctgattta ttatggatat gatcttgttt ataagcattt tgcacaagaa ttacaacgta
    26641 ataatctctc gatgtatccg cttgatcctt atgtatatta cctgattggt gctttgtttg
    26701 tcataggtat tatgattggt tcgcttggtt cagtgctttc tatgagacgt tatttaaaat
    26761 tctaaaaaaa gcgctgctga cgaaacgtag cgaccctcaa aagttggacc tcttatgtta
    26821 ttccaaggac ttcgttttgt attgcacagg actggtcctt ttcattttgt tttgattcat
    26881 ttgtttattt gtaataggca atatagttta aaatagcagt ttctaaggtt tcaagagaag
    26941 aaaagcaccc caaaagttag atgtttgatg tatctaactt ttggggtgtc attcaaaatt
    27001 tagtggctct tttttagtaa aatactatga tttcagtttt aggataacct caaacagtat
    27061 ctaaaatagc tatgtcaatg aaaaaagggg ttagcatttt tttcgtgaca aatggtagta
    27121 gaaggaccat gcccaggata aactcggtaa tgattaggta aggtgaatag ttcttggcga
    27181 ataccagtaa tgaggtcctc aaagttactg gtaggtaagt cagtacgacc gatagtttca
    27241 cgaaatagag catcgccagt aacgactaat tcatctgagt ggaaaacaaa tgagactcct
    27301 ccccaagaat gaccaggtgt tggtaaaact gtgaattcaa aaccatttag ttgatagggt
    27361 tgctttaatt taaaaaagtt ctctgccggt cttgctataa cagtaatgat atcatcatga
    27421 cggcctaacc cagataaatt atcatctggg gaagatagcc aagcggcttc tttttctgaa
    27481 acataaacag gtggatgatc aaaagcatct ctgaccaaat ctaaactaaa aatatgatca
    27541 taatgagtat gcgttaagag gatagccact aaaggttttt caaaggattt aattttagca
    27601 atgatttctt ggccgttact tcctggatca atgaggataa cagcttgatc attaacgagg
    27661 taataagtat tttcaccagc aatgtggttt attgttttat agattttcat aaggatagtg
    27721 taccaaaaaa tcaaggcttt gttaaaaaaa gagtggcgga aaataaatgg caattagttt
    27781 cagaaaatat gagatctcac agcaatttct tgacaccgtt ttcataaagg tgtatcataa
    27841 aagtaatcaa taagccaagc ctaaagctga acttagttta agaacactga ctttttgaga
    27901 gcaacctaat cggttgacta aaaaatatga ggtggaagat atgtcaaaag ttgctattgt
    27961 tactggtgct ggtcaaggaa ttggatttgc tattgcgaaa cgtcttcatg cagacggctt
    28021 taaaataggc attttagact ataatgaaga gactgctcag gctgcggttg ataaactatc
    28081 acctgaggat actgttgcag ttgtagcaga tgtttcaaaa cgtgaccaag tttttgatgc
    28141 tttccaaaaa gttgtagata cttttggtga tttgaatgtt gttgttaaca atgcaggtgt
    28201 tgccccaaca acccctcttg acacgattac tgaagagcag tttgaaaagg cttttgcaat
    28261 caatgtcgga ggaacgattt ggggaagcca agcagctcaa aaacatttta gagaattagg
    28321 tcatggcgga aaaattataa atgcaacgtc acaagcagga tgtgaaggga atcctaattt
    28381 gactgtttac ggtggtacaa aatttgcagt tcgtggtatt acacagactt tggcaaagga
    28441 tttggcatca gaaggaatca cagtcaatgc ttacgcacca ggtattgtta aaacacctat
    28501 gatgtttgat attgcccatg aagttggtaa aaatgcaggc aaagatgatg aatggggtat
    28561 ggagcaattt gcaaaagata tcactttgaa acgcttgtct gaaccagaag atgttgctaa
    28621 tgcagtaggc ttcttagtgg gagatgattc aaattatatc actggacaaa caatagttgt
    28681 cgatggtggt atggtattcc attaaaaatg attaatactg acactgtgag tttcactcac
    28741 agtgtttttt atatttctta aaaaattatc ctatcttgat tattggagtg atggcacctt
    28801 tgaggtatag tcatgttttg ttttatagat atagcgtgct ataatagact tactatgacc
    28861 cagaaaaaac ttagaaaata tgcagtagtg gacttggagg ctactggagc aggtcccaat
    28921 gcttctatta ttcaggttgg gattgttatt atccaaggaa ataaaattat tgatagttat
    28981 gaaacagatg ttaatcctca tgaatctttg gatgagcata ttgttcattt gacaggtatt
    29041 acggataagc agttggctaa agctccggat tttggtcaag tggcgcatca catttatcaa
    29101 ttgattgaag attgtatttt tgttgcccac aatgtaaagt tcgatgcaaa tttattggca
    29161 gaagctcttt ttttggaagg atatgagtta acaataccac gtgttgatac tgttgaatta
    29221 gcccaactat ttttccctcg ctttgaaaag tataacctta gtcacctatc taggcagctc
    29281 aatattgatt tggcagaagc tcatactgct attgcagatg caagagctac agctatactg
    29341 tttttaagac ttttacaaaa aattgagagc cttccaattg agtgcttaga aagcttatta
    29401 gcctattcag atagcctgct atttgaaaca gcaatggtca ttcaagaagg attagccaag
    29461 gccaaacctt atgatcccaa caaatacatt aaaatcagac agattctctt accaaagggc
    29521 tcaaaagctc tcaagcctta tcaaatatca aaaagcttcc ctattaatat ggcactatta
    29581 ggtctggaag aaaggcctaa acagactcaa tttgctcagt taattgacga agattaccat
    29641 cagggagtag ctagttttat tgaagctcag acaggtattg gaaaaaccta cggctacctg
    29701 ctaccccttt tggcaaaaga agatcaaaac caaatcattg ttagtgtgcc aaccaaactt
    29761 cttcaagacc aattaatggc tggtgaagta gctgctattc aagaacaatt tcacatcgcc
    29821 tgccatagtt taaaagggcc tgccaattac ctaaagctag atagctttgc agatagtttg
    29881 gaccaaaatg accagaaccg tttggtgaat cgttataaaa tgcaattatt ggtttggctc
    29941 ttagaaacta agacaggaga ccttgacgaa atcaaacaaa agcagcgctt tgccgcttat
    30001 tttgagcagc ttaagcacga tggagacatt aagcagtctt ctgaatttta cgattatgat
    30061 ttttggagag ttagttatga aaaagccaaa acagcccgtc tcctgattac caaccatgct
    30121 tattttttac accgtgttca agatgacaag gactttacta gaaacaaggt cttggtattt
    30181 gatgaggccc aaaagctgat gctccaatta gatcaattat cacgtcatca gcttaacctg
    30241 acggtttttt tacaaacaat acaggccaag ttaagcaacc ctttacctct tttagaaaaa
    30301 cgcttattgg aaagcttgtc gtttgaactg ggacaagtgt caagtgatta ctatcaaaat
    30361 aaagagcacc agcttgctca tgattggtct cgtatagctg gctacgccaa agaattaaca
    30421 ggagcagatt atcaagagtt acaagccttt tttgctacct cagatggtga ctactggctt
    30481 agtagcgaaa agcaagagga gaagcgagtg acttatctaa acagtgctag taaggcattt
    30541 attcatttcc aacagctctt accagaaacg gtgaaaactt attttgtttc tgccacttta
    30601 accatcagct cagaagtaac cttggcagac ctattaggtt ttgaagagta tctctatcat
    30661 gtcattgaaa aggataaaaa gcaagaccag ttggtgcttg ttgatcaaga ggcgcctatc
    30721 gtgacagaag tcagtgacca aatctatgtg gaagcaattg caaaacgaat cgaaagcttg
    30781 aagcaagagg gttatcctat tttagtgctt tttaattcta aaaaacactt gctgttggtt
    30841 tcagactatc tagaccaatg gcaagtgcct cacttagcgc aggaaaagaa tggaacagct
    30901 tataatatta aaaaacggtt tgatcaagga gagcaaacta ttttgcttgg tttaggttct
    30961 ttttgggaag gagttgattt tattcaagcg gatcgtatga ttacccttat cgcacgattg
    31021 ccctttgata acccagagga tttctttgtt aagaaaatga gtcattatct attggaaaaa
    31081 ggtaaaaatc ctttcagaga ttacttcttg ccaatgacta ttttgcgatt aaaacaggct
    31141 attggtcgca cgatgcgtcg acaagatcaa aagtcagtgg ttattatttt ggatcgtcgt
    31201 ctccttacaa agtcctatgg acaagttatt ttggaaggtc tcggtcagga atttttgatt
    31261 tctcaacaaa attttcacga ttgtctagta gaaacggact gttttctgat ataatggaaa
    31321 ggatctatta tatcagaaaa atttttaatg gcgttaagca caatcaacta acatttaaat
    31381 tagtttgaac ttttattatg ctcttttaga caactgttca aatgttttcg aattgttttt
    31441 ttgataagga caataaatga acaaattttt ggttttagta aacccaatta ctgttctttt
    31501 cattaatgtc agttgctatc aaaacagctt gtcatagttc gtgcgatcag tagcgagtat
    31561 tggtaaaact gaaaccgtag attatgaaaa aatgatctgt tgatcattaa ttaatctgaa
    31621 gataagaagt cgataaaaag gagatacgat gcccaaacta tcaaagcgag ttttagagat
    31681 gaaagagagt gttactctgg ctgcaggtgc tagagctaag gctttgaaag cgcagggtcg
    31741 tgatgtactg aatttaactt taggagaacc agattttttt acacctaagc acattcaaga
    31801 caaggcgatt gaatctattc aaaatggtac ggctagcttt tatacaaatg cttctgggct
    31861 tccagaatta aaagctgcga ttgccacata cctgaaaaat cagtatgggt atcacttgtc
    31921 tcctgatcag attgtggcag gaacaggtgc caagttcatc ctttatgcct tttttatggc
    31981 tgttttaaat cctggtgacc aagttctcat tccaacacca tattgggtat cttattctga
    32041 ccaagtaaaa atggcagaag gtcagccgat cttcgttcaa ggactagaag aaaatcaatt
    32101 caaggtaact gttgaccagc tggaaagagc aaggacaagt aagactaaag ttgtccttat
    32161 caattctcct tccaatccaa caggcatgat ttatggtgct gaggaattaa gggccattgg
    32221 agaatgggcg gtgcacaacg atattcttat tctggcagat gatatttacg ggtccttagt
    32281 ctataacggc aatcagtttg ttcctatttc aacactctca gaagcgattc gtcgtcaaac
    32341 cattacggtc aatggcgtgg ctaaaagtta tgccatgaca ggctggcggg ttggttttgc
    32401 agcaggtgaa ccagagatca tttcggcaat gagtaagatc atcgggcaga ctacctctaa
    32461 cctgacaacc gtttcacaat atgctgctat cgaggctttc tgtggtagtc agtcatcact
    32521 tgaagagatg cgattagcat ttgaagagcg ccttaatatc acttatccat tgttgtgtca
    32581 ggtccctggg tttgaggttg ttaaaccaca aggagctttt tacttctttc caaacgttaa
    32641 gaaagccatg gaaatgacag gtttttccga tgtgacaagc tttgctaatg ctattttaga
    32701 ggaagtcgga ttagctgttg tgagtggagc aggctttggc gcaccggaaa atgttcgtct
    32761 gagttatgct acagatatcg aaacccttaa agaagctgtt cgtagactgc atgtctttat
    32821 ggaaagtaat gaaattaatt aaattaatta aataaaaata gagagaaact tatgtcaaaa
    32881 aaacttattt caattgttga tgttaaagat tatgttggtc aagaagttac tattggtgct
    32941 tgggttgcta acaaatcagg taaaggtaag attgcgtttg tacagttgcg cgatggttcg
    33001 gcttttttcc aaggtgtcgc ttttaaacca aacttcattg aaaaatatgg tgaagaatca
    33061 ggtcttgaaa aatttgacgt tattaaacgt ctcaaccaag aaacatctgt ttatgtaaca
    33121 gggattgtta aagaggatga gcgttctaaa tttggctatg agcttgatat tactgacctt
    33181 gaaatcattg gagagtcaca tgaatatcct ataactccta aagagcatgg gacagacttc
    33241 ttaatggaca accgtcatct ttggttgcgt tctcgtaaac aaatggctgt tatgcaaatt
    33301 cgtaacgcta ttatttatgc aacttatgaa ttctttgacc aaaatggttt tatcaaattt
    33361 gatagcccaa ttttatcaga aaatgcagca gaagattcaa cagagttatt cgaaacagac
    33421 tattttggga aaccagcatt tcttagccaa tctggtcaat tataccttga agctggagca
    33481 atggctctag gccgagtttt cgactttggt ccagtctttc gtgctgaaaa atctaaaaca
    33541 cgtcgccatt taacagaatt ttggatgatg gatgctgagt attctttctt atcacatgag
    33601 gaatcacttg accttcaaga agcttatgtt aaagcattga tccaaggcgt tctagatcgt
    33661 gcgcctcaag cacttgatat tttagaacgt gatgttgagg ctctcaaacg ttatatcaca
    33721 gaaccattca agcgtgtttc ttatgatgat gctattacac ttcttcaaga gcatgaagca
    33781 gatgaagata cagattatga acatttagag catggtgatg actttggttc cccacatgaa
    33841 acttggattt ctaattactt tggagtaccg acatttgtag tgaattaccc agcaagcttt
    33901 aaagcatttt atatgaaacc agttcctgga aatccagaac gcgttctttg tgctgatctt
    33961 ttagctccag aaggttatgg agagatcatt ggcggctcaa tgcgtgaaga caactatgat
    34021 gctcttgtgg ctaaaatgga tgaactaggt atggataaat cagaatatga cttttacctt
    34081 gaccttcgta aatatggctc tgttcctcat ggcggttttg gtataggtat tgaacgtatg
    34141 gtaacttttg ttgctggtac caaacatatt cgcgaagcta ttccattccc acgtatgtta
    34201 catcggatta gaccctaata atacaaaaaa gttcatgtca tctatgaact ttttgtattg
    34261 gtaagaggtg agtgtgttat aaaaaaatca atatgtccta aaggagagat aagaaatagt
    34321 agagtttgaa cctacatcct ttgtgaagat aaattttgga agtttagtag atcagttagc
    34381 agataagaca atatagcctt gctcaaggga tattttatag acaagtattt ggactacgat
    34441 tatcaaatag acagtctcat ttgatactaa aaaggtctcg tctattcata taataggaga
    34501 gttacgggag ttattattaa aattagctga tttttacagt tcaaaatgtg ttaccacttt
    34561 ttcacttctt agctttctta tgtatgtggt ataatgaagg tggacggttc gatagagaaa
    34621 gggaaattgt aaatgtcaga caaacacatt aatttagtta ttgtgacagg aatgagcggc
    34681 gctggaaaaa cagttgccat tcagtctttt gaggatctag gctactttac cattgataat
    34741 atgcccccag ccttggttcc aaaattttta gaattaattg aacaaaccaa tgaaaatcgt
    34801 agggtggctt tggttgtcga tatgagaagt cgtttgtttt tcaaggaaat taattctacc
    34861 ttagatagta ttgaaagcaa tcctagcatt gattttcgga ttcttttttt ggatgcaacg
    34921 gatggagaat tggtgtcacg ctataaagaa accagacgga gccacccttt ggctgcggac
    34981 ggtcgtgtgc ttgatggtat tcgattggaa agagaactcc tatctccttt gaaaagcatg
    35041 agccaacatg tggtggatac aacaaaattg acccctagac aattgcgtaa aaccatttca
    35101 gaccagtttt ctgaagggtc taatcaagcc tctttccgta ttgaagtgat gagctttggg
    35161 ttcaaatatg gtcttccttt ggatgcggat ttggtttttg atgtgcgttt tctacccaat
    35221 ccttattata aggtagagct tcgtgaaaaa acaggactag atgaggacgt ttttaattat
    35281 gtgatgtctc acccagaatc agaggtgttt tacaagcatt tgttaaacct tattgtccct
    35341 atcttaccgg cttaccaaaa agaagggaag tctgtcttga cggtggctat tggctgcaca
    35401 ggaggccaac accgcagcgt tgcctttgcc cattgcttgg cagaaagtct ggcaacagat
    35461 tggtcggtta atgaaagcca tcgtgatcaa aatcgtcgta aggaaacggt gaatcgttca
    35521 tgaaaaatcc taaaatgact gttataggtg ggggtactgg catctccatt atcctaaaaa
    35581 gccttcgtaa tgaagcagtt gatattactg ctgtggtcac tgttgcagat gatggtggtt
    35641 cctcaggaga acttcgtaat gccatgcaat taaccccgcc aggagactta cggaatgtct
    35701 tgttagcgat gagtgacatg ccaaaatttt atgagcgggt tttccaatac cgttttaacg
    35761 agagtgatgg agccttggct ggtcaccctc taggcaatct cattattgct ggtatctctg
    35821 aaatgcaagg ctcaacctac aatgccattc aaattttaac caaatttttc catattacag
    35881 gtaaaattta cccctctagc gagcaggcct tgacccttca tgctgttttt aaagatgggc
    35941 acgaagtagc aggagaaagt tctattgcca aatatcaagg catgattgac catgtctatg
    36001 tgaccaacac ctacaacgac caaaagcctc aggccagtcg taaggtggtt gaggctattc
    36061 tagaaagtga catgattgtc ttgggaccag gctcactgtt tacctctatt ttacctaatt
    36121 tggtcattcc agaaattaaa gaagctttgc gccagactaa agcagaagtg gtgtatattt
    36181 gtaatatcat gacccagtat ggtgaaacag aacaattttc agatgcagac catgtggctg
    36241 ttttaaatca gcacttagga agagatttga ttgatacggt tttggtaaat gtggctaagg
    36301 tgccgcaggc ttacatgaat tctaacaaat ttgatgaata cctcgttcag gttgatcacg
    36361 actttgctgg cctttgcagg gctgctaagc gtgtgatttc gtcttatttt ttacggttgg
    36421 agaatggcgg agcttttcac gatggtaact tagtggtgga agaattaatg aatttagtga
    36481 ggatagtcaa gcaatgagtt ttacaaccaa ggtcaaagag gaattaattc acttgtccac
    36541 aggtgacaac aatgaattgg ccgccattat caaattatca ggcagcttgg gcttagctca
    36601 ccaaagcctt catttatcca ttaccactga aaatgctaaa attgcccgct acatttattc
    36661 tcttattgaa gacgcctatg ttatcgtgcc agaaatcagg tatcatcaaa agacgaacct
    36721 gcgcaaaaac cgtgtttaca ccgtctatgt tgagcaaggt gtggaaacca ttttagccga
    36781 tttgaaatta gcggactcct tttttggtct ggaaacaggc atcgaacctc aggttttatc
    36841 agacgataat gctggccgtt cttatcttaa aggggctttt ctagcggcag gtagcatcag
    36901 agatcctgag tcaggcaagt atcagttaga aatctattca gtttatcttg atcatgctca
    36961 agatttagct caactcatgc aaaaatttat gctagatgcc aaaaccatcg agcataaaag
    37021 cggtgcagtt acttatgtgc aaaaggcaga agatatcatg gactttttaa ttataattgg
    37081 tgccatgtcc tgcaaagaag actttgaagc catcaaatta cttcgagaag ctcgaaatga
    37141 tattaaccgt gccaataatg cagaaacagc taatattgcc aaaactatca gtgctagcat
    37201 gaaaaccatt aataatatta ttaaaatcat ggacacaatt ggtttagaaa gtttaccgat
    37261 tgaattgcaa caggtagccc agcttcgggt taagcatcca gactattcca ttcaacaagt
    37321 tgctgatgct cttgagttcc caatcaccaa gagcggggtc aaccatcgct tgcgtaaaat
    37381 caataaaatt gcagatgact tatagaacat ttttatcgta tgagcctttg ttcatacgat
    37441 ttttttgtct aaaagtcgtt ttccattgtt tttttgaagt gtctgatgtt ctgtgatatg
    37501 ataaaaggga taataacgtt tgtaaaggag actgataatg gcatgtacaa ctatactcgt
    37561 cggtaaaaag gcatcttatg atggctcaac catggttgct cgaacagaag attctcaaaa
    37621 tggtgatttc acgcctaaaa aaatgattgt ggtgaaacca gaagatcaac cgagacatta
    37681 ccgttcggtg caatcttcct ttgaaatgga cttaccggac aatcccatga cttacacgtc
    37741 tgttcccgat gcgcttggta aagatggtat ctgggcagaa gcaggcgtga acgaggctaa
    37801 tgtggccatg tcagcaactg aaaccatcac taccaattct cgtgttttag gggcagatcc
    37861 tctagtagct tctggtattg gtgaggagga catggtgacc ttagtgctcc cttatatccg
    37921 ttctgcgcgt gaaggggttt tgcgccttgg tgccatttta gaagactatg gcacttacga
    37981 gtcaaatggt gtggcttttt cagatgaaca tgacatttgg tggctggaaa cgattggtgg
    38041 tcatcactgg attgcaagac gggtgccaga tgatgcttat gtcaccaatc ccaatcaatt
    38101 tggcattgat tattttgaat ttaacaatcc tgaagattac ctttgctcag ctgatttaaa
    38161 ggactttatt gacacgtatc acttagattt aacctataac catgagcatt ttaatccccg
    38221 ttatgccttt ggtagtcaac gtgacaaaga ccgtcactat aatacgccac gtgcttggat
    38281 catgcaaaaa ttcttaaacc cagaaattgt gcaagacccg cgcagctttg cccttgcttg
    38341 gtgtcaaaaa ccttaccgta aaataacagt agaagatgtc aaatatgtat tgagtagtca
    38401 ctatcaggac acggtttatg acccttacgg ttcagaagga actcctgtta gtaagaaagt
    38461 ctttcgccct attggcatta accgtaccag ccaaaccgct atcttacaca ttcgtcctaa
    38521 taaacctcaa gaaattgcgg ctatccagtg gatggcttat gggtcaatgc catttaatac
    38581 catggtgcct tttttcacac aggtgaagac tattccagat tattttgcca acacttacga
    38641 aaatgtgtct accgacaatt tctattggac caaccgtctg attgcggctt tggcagatcc
    38701 tcactacaat catcatgaaa ctgatttgga caattacttg gaagaaacca tggcaaaagg
    38761 tcatgccatg ttacatgctg tggaagcaca gttattggct ggtgaaacag tcgatttgga
    38821 agaggaaaat caaaaaatga gtgactatgt gcaaggagaa acgcagacgc tgttgaacaa
    38881 gattctcttt gacgctagca atctaatgac caatcgtttc agtttgagcg attaaaagtg
    38941 aagctaaggc tgagcaactg cttagccttt tttacgataa ctttttagga aaagtaatcg
    39001 caaaaagaca aaaacttgga aaaaaaatca aagtatgcta tacttaactg gtcaattttt
    39061 aaccagttaa taacaaagga ggaatcatga aaaagaaaat tcttttaatg atgagtttaa
    39121 tcagtgtctt ttttgcttgg caacttactc aggcaaaaca agtcttagca gagggtaaag
    39181 tgaaggtggt gacaactttc tatcctgttt atgaatttac aaaaggggtt attggtaatg
    39241 atggcgatgt ttccatgctt atgaaagcag gaacggaacc tcatgatttt gagccttcta
    39301 caaaagacat taaaaaaatc caagatgcag atgtatttgt ttatatggat gacaatatgg
    39361 aaacttgggt ttctgatgtg aaaaaatcat tgacatctaa aaaagtgacc atcgtcaagg
    39421 gaactggtaa catgctcttg gtagcaggag ttggacatga ccatcaccat gaggaagctg
    39481 acaaaaagca tgagcataat aaacatagcg aagaaggaca caaccatgct tttgacccac
    39541 acgtgtggtt gtcaccatac cgtagcatta cggtcgttga aaatattcgc gacagtcttt
    39601 caaaagctta cccagaaaaa gcagagaact tcaaagccaa tgccgctact tatattgaaa
    39661 aattaaaaga gcttgacaaa gactatacgg cagcactttc agatgctaag caaaagagct
    39721 ttgtgacaca acacgcagct tttggttata tggcacttga ctatggcttg aaccaaattt
    39781 ctattaatgg tgtcacacca gatgcagaac catcagcaaa acgtattgct actttgtcaa
    39841 aatacgttaa aaaatatggt atcaaataca tttattttga ggaaaatgcg tcaagtaaag
    39901 tcgcaaaaac cctagctaaa gaagcaggag ttaaagcggc tgtgcttagt ccgcttgaag
    39961 gtttgactga aaaagagatg aaagctggcc aagattactt tacggtcatg cgtaaaaacc
    40021 ttgaaacctt acgcttaacc actgatgtgg ctggtaaaga aattcttcca gaaaaagaca
    40081 cgactaagac agtttacaat ggttatttca aagacaaaga agtcaaagat cgtcaattat
    40141 ctgactggtc aggtagctgg caatctgttt acccctatct acaagatggt actttagacc
    40201 aagtttggga ctataaggct aaaaaatcta aaggtaaaat gacagcagcc gagtacaaag
    40261 attactacac tactggttat aaaactgacg tggaacaaat caaaatcaat ggtaagaaaa
    40321 agaccatgac ctttgttcgt aatggtgaaa agaaaacctt cacttacaca tacgccggca
    40381 aagaaatctt gacctatcca aaaggaaatc gcggagttcg tttcatgttt gaagctaaag
    40441 aaccaaatgc tggcgaattc aaatacgttc aattcagtga ccatgccatt gctcctgaaa
    40501 aagcagagca tttccacctg tactggggtg gtgacagcca agaaaaatta cataaagagt
    40561 tagaacattg gccaacttac tacggttcag acttatctgg tcgtgaaatt gcccaagaaa
    40621 tcaatgctca ttaatcttaa ctcactaagc aaatcttctt ttaaggaggt ttgctttttc
    40681 tttgtggagt aaaaaaattt aataattctc aacattaatt tgacggacac ttatgattat
    40741 aatatacttt aagtggttat aacaagttag aggtgaataa gatgccaaaa gaacaaccac
    40801 tctatctaca gattgttgat gacttggaag tgaaaatcaa aaagagcatg acagaaaacg
    40861 ataagttgtt gtcagaacga gaattaagtg acctttacgg tgttagtcgt atcacgatta
    40921 gactggctct taaggaattg gaattacgag acttaattta taaaaagcag ggcaagggga
    40981 cttatgtttc aggaatcaaa gaacctgtca ctgatttatc aagtacttac agctttacag
    41041 agcaaatgaa aaaagtgggg aaaactccta aaacagagat tctttctttt gaacagtatc
    41101 aggtaacacc ttacttgtct ggtctcttag agtttgatct cgatacagaa gtttttgaat
    41161 tagaacgcct aaggattgca gatgatatgc ccttgatgtt tgaacgatcc tatattcctg
    41221 cgcagccttt tcaggggtta agcattgcgg acttgaagcg taaagctttg tacgatatct
    41281 ttgcaaagga ctatcaagag acaatccgtc ttgcagaaga agagttttat gccagcattg
    41341 ccttggacta tgaagcagac cttttgggaa tcaaaaaagg agatcctgtc ttacatatta
    41401 ttcgtaaaac ttataacgat aaaaatattt tgatcgaatt tactttcagt attgcaagag
    41461 cagaccagtt taggtatagg gtacaacatc accccaatgg ttaaagaaat gggaggttaa
    41521 tacatgttta caaaaacgca ggaagctttg gaggctcttg gtgcagctat cactaccaag
    41581 gaaattaaac aagaaccccg tttatggcaa gaaaccatga cgttttttga agaaacaaga
    41641 gattctctgg attctttttt aaagagagtt tgtaaaagcg ctaatggaaa taacgttcat
    41701 gtcatcttta caggtgcggg tacatctgag tacatcggta ataccatttg cccttacttg
    41761 aagaaggtgg ggaatcggca gtgctatctc tttgagagtg ttgcttcaac cgatttggtt
    41821 gcggcgccag attattatct tgctgaggaa gagacggtac tattagtctc ctttgctagg
    41881 agtggtaata gtccagaaag tgtagcggct gttaacctgg tcaatcaact agtgccaaat
    41941 agttatcatt taaccattac gtgtgccaaa gatggagaac tggcgaaaaa agcacagcaa
    42001 gatgagcgtt cttaccttta tttgatgcct gaagacgcta atgatgctgg ttttgccatg
    42061 actggtagtt ttacctgtat gatgctagcg gctcttttga tttttgatga tgctaacagt
    42121 atagctcaga aagaagctta tgtgactgat atgattcttg ctgggcaagc tgttattgac
    42181 caagaaagac ggctacaaga gctagctgat ttaggatttg aacgtttagt ttatctaggt
    42241 tctgctggtt tagccaagtt aacccaagaa gctcaattaa aaatgttaga attgacagct
    42301 ggacaagttg cgaccctcta cgagtcctct atgggcttta ggcatggtcc gaaatctttt
    42361 attaatgacc gtaccttagt cattggattt gtcaataatg atgcttacgt cagacagtac
    42421 gatttggaca tgttagagga aatccaagca gatggcattg cccttaaaat cttggctcta
    42481 ttgcaaggag gggatatcaa cttttcagag gaccaatttc gactagacac ccgtcactta
    42541 ttgccagaag cttacttagc ttttcccatg attttagtcg cgcagacctt ggccctctta
    42601 acggccgtta cattaggaaa ttcccctgac accccatctg cgacaggaac ggttaatcgt
    42661 gtggtcaaag gagtaacgat tcacccttac ccgactagta gtcgtcaatg caacaaagct
    42721 taaagctttt gtgtagaagc cgtaaaacga aatgattttg tgctagtagg ccaaaagaaa
    42781 aaccctcctg atacaggaag gttttttgta ttgtcttgct tattttgctg cgtttgcgtc
    42841 tttgagaccg tattttttgt tgaagcggtc cacacgtcca tctgcttgag tgaacttttg
    42901 acgtcctgtg tagaatgggt gtgaatctga tgaaatttcc acacggataa gtgggtaagt
    42961 ttcaccttca aactcaacag tttctttaga agcttttgtt gagccgctaa ggaactggta
    43021 acctgtagtt gtatcaagga aaacaactgg acgataatct ggatgaatgt cttttctcat
    43081 cttaaaaaat tcctttctgc catgacactt gatgcatcat agttattaac tattctagtg
    43141 taacaaaact tgaatgactt gacaaggctt ttctttcctt attttttaaa aatcctaaat
    43201 ctcttggcaa acagcgatga gttctcggaa aatagcttga ttttcttcta aattagctga
    43261 gttggctccg ctagcaaggg gatgcccccc tccaccgtga cgcttagcaa tgccattaat
    43321 aatcttttct ttactgcgca tacgcacacg gtagttgccg tcagttaact caacaaaaat
    43381 agcccaagct tgaacgttat caattttacc aggagcacag acaatggcag agctttctgc
    43441 tagggtcacg tcaaaatgtt ttaaggtttc ttggctgaca aggacataag cagccccact
    43501 ctcatcaatt gttaaatgct caaagacgta gctttgcagc tttgctattt tcaaaggaaa
    43561 cgaatccatt tgccttgaaa tcgcagcaaa gtcgaactcg aaatgtctga gttggctagc
    43621 aatggaaagg gttttactag tggttgaggc ataaagaaat cgccctgtat caccaacgat
    43681 accagtgtat aagagcttag cagccttgtc agagagagta agattctgac taaaggcaaa
    43741 gtctgcaatg atttcacttg cgctagaagc gcttgtgtcc acataataga agtcaccata
    43801 tacatcatcg ttgggatggt gatcaatctt aattaagcac tgccccagtg tgtagcgctc
    43861 atcatcaatc cttggtcgat tcgctgtatc tgtaatgatg accaaagcct ctttatagtc
    43921 tttatcagtc acctgatcca tttggctaat ccaagttaaa ctaggctcat caaaaccagt
    43981 catcaaaacc tttttatctg ggaaattttg tgcaataatt tctttcaagc cggcctgact
    44041 accaagagca tcagggtcag gattttgatg gcgatggata ataatagttt ggtgagcttt
    44101 tattttatct aaaattgttt caaaagttgt tatcatgctt taatctttct ttttcaaaaa
    44161 gaggaaaata accgtttaac ataatcttca aaaaacggtt tgtgtcataa ggaggtgtgc
    44221 gttatgttaa gtggctaatg ctagaatttt ttaagattgt caaagctcat aaattgacat
    44281 tttatctgaa aatcatatcc tattttatta tagcataaaa gtaagctcta gataatggta
    44341 aattaatcta ggaagaaaag aggttagtta tcagttatga tactatcaaa gctagttttg
    44401 caaatgcaag tgaaaaaata gtttttaagt gtcttaagta atcatatcaa ctttctcttt
    44461 aaacaattag tgcttttcct aaaaaagtgt gctaaaataa acatagttta gtaattttgg
    44521 gaggaattta tggcattagc aaaaatcgtt tatgccagca tgacgggaaa tactgaagaa
    44581 atcgctgata tcgttgcaaa taaattgcaa gaattgggcc atgatgttga cattgatgag
    44641 tgcacaacag ttgacgcttc agagtttgag aatgctgata tcgctgtcgt tgcgacttat
    44701 acttatggtg acggtgactt accagatgaa atcgttgatt tttacgaaga tttacaagat
    44761 ttagatttag aaggtaaaat ctatggcgtt gtcggctctg gtgacacttt ttatgactat
    44821 ttttgcaaat ctgttgatga cttttcagat caatttgcgc ttactggtgc cgtcaaaggt
    44881 gcagagccag tcaaagtaga tttggcagca gaagatgaag acatcgatcg tttagaggcc
    44941 tttgcagagc aactctcaca ggctgttaat aatctataaa actatttagt aaaaatgtca
    45001 gggacttcct tggcattttt agatttttaa cctaaaataa agggaatgtc acttcagggt
    45061 aggaattgaa aaaacaatca gcttcacctt gcttctgatc ctcttattaa ctataataaa
    45121 aagataatct aatacatcta gaaaaggaaa gttatgcgtt tagaagagat acgacaagaa
    45181 atcaatggta ttgatcacca cttggttgcc ttattagaaa agcggatggc acttgtagag
    45241 caagtaacag cttataaact agccaatcac ttgcctgtct tagatcaggc gagggaaaat
    45301 cagatcctag atagggtttc ttatctcgtt aaagaccaag catttgagcc agctatccat
    45361 gagactttta aaaccattat gtctctttca cggcagtatc aaacgcaacg cttaacaggt
    45421 ggtgacatta atgattaaat caattgcaac actgcctaaa ccctccttaa aagaagcgtt
    45481 aggactaata ggcttggcgc ttcccatagg ggccacggtt ggagtggttg atgtgatttt
    45541 tggtaaaggg cttttgtttc tttccgaata tcgcgatcac cacctgttct tgcttcttcc
    45601 ttttcttgcc ctagcaggtc ttgttatcgt ttttctttac gataagttag gcaaagaggt
    45661 tcgtcaaggg atgggattgg tttttcaggt aggacatggt caaaaagatc agattcctcc
    45721 tattctcatt cctcttattt tgttctcgac ttgggtaacc cacttatttg gagcgagtgc
    45781 gggtcgagaa ggggtagccg ttcaaatagg agctaccatc tctcattatt gtcagagatt
    45841 tgtgactagt caagaagccg cacgacacct tttgattatg gggatggcag ccggttttgc
    45901 aggacttttt caaacgccga ttgctgctgt cgtatttgcc cttgaggttt tgttggtagg
    45961 aacgcttcgc tactgcgctt tattgccttc cttagttgct gcttgcgtgg cttcttggac
    46021 ctctcattct ttgggattag agaaatttac gattgttctt gaagaaacat tgaccataac
    46081 tcccttaaca ttggttaaat taattgggtt aggccttatt tttggtctgg tgggaaatag
    46141 ttttgcctat ttacttggct ggtttaaacc ttacctcagt caaaagctac caaatcctta
    46201 tttccggatt gcttttatcg gtgctttgtt atctatctgt ctgatgattg gtcacgtcgg
    46261 aagatatagt ggtctaggaa ctcatttgat tgcggcagct ttttctggcc aaactatcct
    46321 aacttacgac tggcttttaa aaatgatcgt gacggtgatt agcttatctg caggctttca
    46381 aggtggtgaa gtgacgcctc tatttgctat tggagcttct ttaggcatcg ttttggcccc
    46441 ttacttaggt cttcctgtac tgttggttgc tgcacttggt tacaccacgg tatttggaag
    46501 tgcaacaaat accttttggg cccctatctt tattgggata gaggtctttg gtccagagaa
    46561 tgccctagca tattttgtca cctcggctgc agcctatatg gttagtcaca gacattccat
    46621 ttacagttat caaaaagtag tcatgatcaa tttaaaagac atgacggtaa atcagatgga
    46681 tttaaaaaaa tagctttaca ataaagaagc tttttgttag aataaatctc gtgtgtaatg
    46741 gacacacgaa aattacggct aatccgctaa gacaagtact taagattagt aagataggag
    46801 aataaaaatg aatccattaa tccaaagttt gacagaaggt caacttcgct ctgatatccc
    46861 taacttccgt cctggtgaca ctgttcgcgt tcacgcaaaa gttgtcgaag gaactcgcga
    46921 acgtatccag atctttgaag gtgttgttat ttcacgtaaa ggtcaaggga tctcagaaat
    46981 gtacactgtt cgtaaaatct caggtggtat cggggtagag cgtacattcc caatccacac
    47041 accacgtgtt gacaaaatcg aagttatccg ccacggtaaa gtccgtcgtg cgaaacttta
    47101 ctacttgcgt gcattacaag gtaaagctgc tcgtatcaaa gaaattcgtc gttagtttga
    47161 accgaaacgt aacgtaatag aataaaaaga ctagagtctc tagtcttttt atgtcgtcgt
    47221 gacaatgtga caattggtga ttaacgctat gctcgtagga cactttgaaa gttccttagc
    47281 agttataatt agtatattgt aataacaaaa tgaagcaaaa taaactttca gttatctgat
    47341 atttcatctt gcattacctt attttctagg ttaaaataga agacaaggtg aatataagat
    47401 aggcccctta gttcaatgga tataacaact ccctcctaag gagtagttgc tggttcgatt
    47461 ccggcagggg tcattattgt cactgaaaaa gcctttacat caaaggcttt ttgttttatt
    47521 ccggttttaa aaggggcata aaaggggcag tttgtttatt tataatttct ttcatgttta
    47581 cagttgtgtg actgtaaata gataatgttg tttttggatc gctgtgacca actctatcca
    47641 ttatcgcatt tagcggtatc cctttttctg ctaaaaatga tatatgcgag tgtctaaata
    47701 agtgcgtgtg ataatctccg taaattttca atcgcttatt gatgtgtgca tttaaaattg
    47761 gtaaaccttt tgaatatgga aagctgttag cactctgctt gtgattaatc tatcgtatgt
    47821 taaaagtgac atttaaatta aatcattttt tatacatata tttcaacagg atttatcata
    47881 ttggaaaata gcggtgcttc tattggcatt ttactattga caggaatttc cctgtctttt
    47941 gtctcctaaa gaagatcagc tttgatttct aacttgatta gagtgggctt ggtactgttt
    48001 atgaattatc agtatatact ttctggagat caaaaaggaa aggaacagct aaagaggtgt
    48061 atgtgttacc attagaggaa tagaacatcg caagactcgt ttattcagtc attacgatac
    48121 ctgttttatt tgggaaataa ctataatatt aagtgaattt gtgatataat gaaaggtatg
    48181 aattatcaag attatatatg ggatttggga ggtaccttac tcgataatta cgagctttca
    48241 acccaagctt ttgtccaaac gctagctttt ttcagccttc caggagatca cgatgctgtt
    48301 taccaaaaat taaaagaatc aaccgctatc gctgtggcaa tgtttgctcc taatgagcca
    48361 gaatttttgc atgtgtatag gctaagagaa gcagacaaat tggcacaacc catttggtgt
    48421 ttgggagcta aagaaatttt ggaaaagata gcgacttcag gttcgcgaaa ttttttaatt
    48481 tctcatcgag attgtcaggt gaaccaactt ttagagcaag ctgggttgct aatctatttt
    48541 acagaagtcg tgacagcttc aaatggcttt gctcggaaac caaatcctga aagtttgttt
    48601 tatttaaaag aaaagtatga tattaatagt ggattagtaa ttggagatcg gctaattgat
    48661 aaacaagcag gacaagcagc gggctttaat accttgcttg ttgacggtag aaaaaaccta
    48721 ttggagatag taacgtagat gattgaagaa aataaacatt ttgaaaaaaa aatgcaagaa
    48781 tacgatgcca gtcaaattca ggttctagaa gggctggagg ctgtccgcat gcgtccaggg
    48841 atgtatattg gctcgacagc taaagagggt ttgcatcatt tagtctggga aattgttgac
    48901 aactcaattg acgaagcatt agcaggtttt gcctctcata ttaaagtctt tattgaagca
    48961 gataattcca ttacagtagt agatgatggc cgtggaattc cagttgacat ccaagccaag
    49021 acaggacgtc ccgccgttga aacagttttt acagtcttac acgcaggtgg taaatttggc
    49081 ggaggcggct ataaggtttc tggaggattg catggtgtag ggtcatctgt tgttaatgct
    49141 ttatcaacac aattagatgt gcgtgtttat aaaaacggcc aaattcatta ccaagaattt
    49201 aaacgcgggg cagttgtagc agatcttgag gtcattggaa ccactgatgt gactggaaca
    49261 acggtacact ttacacccga tccagaaatt tttaccgaaa cgactcagtt tgattacaat
    49321 gttttagcaa aacgtattca agagttagcc tttttgaatc gtggtttaaa aatttccatt
    49381 acagataagc gctcaggtat ggaacaagaa gaacatttcc attatgaagg tggaattggt
    49441 tcttatgttg aatttttaaa tgataaaaaa gatgttatct ttgaaacgcc catctataca
    49501 gatggtgaat tagaaggtat tgcagttgaa gtagccatgc aatacacgac tagctatcaa
    49561 gaaacagtca tgagttttgc taataatatt catactcatg aaggtggaac gcatgaacaa
    49621 ggctttagag cggctcttac tcgggtcatc aatgactacg ctaagaaaaa taaaattctt
    49681 aaagaaaatg aggacaattt gacaggagaa gatgttcgtg aaggtttgac ggcggtaatt
    49741 tctgttaagc atccaaatcc tcaatttgaa ggtcaaacca aaacaaaatt gggcaactca
    49801 gaagtggtta agatcactaa tcgtctcttt agtgaggcct ttcaacgttt tcttttggaa
    49861 aacccacaag ttgctcgtaa gattgtggaa aaagggattt tggcttctaa agctagaatt
    49921 gcagctaagc gagcccgcga agtcacccgc aaaaaatcag gcttagaaat ttcaaactta
    49981 cctggaaaat tagcagactg ttcgtcaaat gatgctaacc aaaacgaact tttcatcgtc
    50041 gaaggagatt cagcgggtgg gtcggccaaa tcaggtcgta accgagagtt tcaagctatc
    50101 ttgcctattc gcggtaaaat tttgaacgtg gaaaaagcaa ctatggataa gattcttgcc
    50161 aacgaagaaa ttagaagtct ttttaccgct atgggtacag gttttggtgc agattttgac
    50221 gtgtcaaaag ctcgctacca aaagctggtt atcatgaccg atgccgatgt ggatggcgct
    50281 catattagaa ccttgctttt aaccttgatt taccgcttta tgaggcctgt tctagaagct
    50341 ggctatgttt atatcgccca gccacctatt tatggtgtta aggtcggtag tgagattaaa
    50401 gagtacattc agccaggtat tgatcaagaa gaccaattaa aaacagctct tgaaaaatat
    50461 agtattggtc gttcaaaacc aactgttcaa cgttataaag gtcttgggga aatggatgac
    50521 catcaacttt gggaaactac tatggatcct gaaaatcgtt tgatggcgcg tgtgacagtt
    50581 gatgatgccg cagaagcaga taaagtattt gatatgttaa tgggagatcg tgttgaacca
    50641 agacgtaatt tcattgagga aaatgcggtt tatagtacac tggatattta gtcacctttt
    50701 tagtttcaca aggaatctgc taccaaagag gacgaattga ctaaaaatca tgatgcatga
    50761 tgccaacagc tttgttagtt agtacgtctt catcttgagg gacagcgtta atgcataggt
    50821 attgagaccg ccccttaata cctatgttag tccttattaa gaaagccttg tgagtcgctg
    50881 gtacgagatt gttgaattag tcaaatatat tttgaaatgc attttaaagt atgttataat
    50941 cgtaatgtta tagtgtattt taaataaaat tttaataaaa ggcgattgag gaatttcagt
    51001 ttttagttat tactggaaca atgttgataa atcgtgtcta aataataagg agattcagat
    51061 gtcaagcgga attatcttgc tgattgtggc tatagtccta ctagtgatta tcgcctatct
    51121 agtgggtgtc atcatacgaa agcgtaatga ttccctaatt accagtctag aagaacgaaa
    51181 acaggccttg tttgctttgc ctgttaatga tgaaattgaa gaagtaaagt cacttcattt
    51241 gataggacag agccaaacct cttttcggga atggaatcag aaatgggtag acttaactgt
    51301 aaattcgttt gcagatattg aaaatcatat ttttgaagct gaaaatttga atgatacgtt
    51361 taattttatt cgcgccaagc atgagattaa tagcgttgaa agtcagctta atcttgttga
    51421 agaagatatc gcttctattc gtgaggcact taatatctta aaagagcaag aagaaaaaaa
    51481 tagtgctcgt gtcacccatg ctcttgattt atatgaaaag ctccaagctt ctatttctga
    51541 gaatgaagat aattttggtt caactatgcc tgaaattgac aaacagatga aaaacattga
    51601 aactgaattt tcacagtttg tcgcactcaa ctcatcaggt gatcctgtag aagcttctga
    51661 agttctggat agagcagaag agcatactat tgctctaggg cagattacag agcagattcc
    51721 agctattgtc gccaagttgg aagatgattt tccagatcag ttagatgatt tggaaacagg
    51781 atatcgccgt ttacttgaag aaaactatca ttttccagaa aaaaacattg aggcacgttt
    51841 tcaggagatt cgtgagtcta ttcgtgccaa ttcatcagag ttagttacac ttgacttgga
    51901 tcgtgctaga gaagaaaaca cgcatattca agaacgcatt gattctcttt atgaagtctt
    51961 tgagcgcgaa attgccgctt ataaggtagc tgctaaaaat agtaagatgc tacctcgtta
    52021 cctagcacat gtgaaacgta ataatgaaca actcaaagat gaaattgcac gtttatcacg
    52081 taaatatatt ctaagtgaaa cggaaagtct gactgttaag gcatttgaga aggatattaa
    52141 agagatcgaa gacagtacac ttgctgttgc agaacaattt ggtttacaag aaaaaccatt
    52201 ttcagaactg caagtgactt ttgaacgcag tataaagacg cttactaatg tcgagtcagg
    52261 tcaaatggat gtttttgcag cagttaaaga tattgaaaaa attgaatcac aggcacgcca
    52321 taaccttgat gtttatgtta ctcagcttca tatgattaaa cgttatatgg aaaaacgtca
    52381 cttgcctgga attccacaag actttcttag tgctttcttt acaacgagtt cacagttgga
    52441 agctttgatg gatgagctta gtagaggacg gatcaatatt gaggcagtgt cacgtttgtc
    52501 ggaagttgcg acggttgcta ttgctaatct agaagattta acctatcaag tggttcaaaa
    52561 tgctaccttg actgaacaat tactacaata ttctaatcgc tatcgttctt ttgaagcagg
    52621 agttcaaagt agttttgaac atgctttgag acttttcgaa gtagaaaatg attatcaggc
    52681 ttcttttgat gaaatttctt atgctcttga aacagttgaa cctggggtaa cagatcggtt
    52741 tgttaactct tatgaaaaaa cacgagagca cattcgtttt tgatacttga aagtcgtgat
    52801 attctcacga ctttttatta gctttttgga ccaatacgtg tttttgacta gaatcttctt
    52861 tttaatgatc aaacgaagaa gtagtaacag tgttttttgg tattgagagg atagttattt
    52921 tttcaaaaat cttttccaaa aggatttggt tttttcagga ggttgtttgc taagttgatc
    52981 attttttgtc tgctttaaga tgacttctaa acgcgtttcc tttagattta ttgcgtggtc
    53041 ggtatgaagg ataaacgcaa aaggtgaagt catatgcgtt tctgtaataa tagtaacagg
    53101 taaatggttt tcttttgcta atttcatata gagtatttga agttgaggtg ccagcttgga
    53161 agagattttt aagtgtaaag gttgatagac taactgaagg ctcggtaatt ctcttttcaa
    53221 atagtctttt actgttttag tttgagcttc gtctaataaa gcacttaaaa cgactcgttc
    53281 agcatacgtt cctagatagt agcgttgctg ctcaggattt aatcttcttt ctccccaggc
    53341 tccttttaat agcttatctt ctaaattagt catagatacc ct
//
LOCUS       DATXIG010000007        50024 bp    DNA     linear   BCT 28-DEC-2023
DEFINITION  TPA_asm: Streptococcus pyogenes strain MGAS11113
            SAMN08555799-rid19682583.denovo.007, whole genome shotgun sequence.
ACCESSION   DATXIG010000007 DATXIG010000000
VERSION     DATXIG010000007.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN08555799
            Sequence Read Archive: SRR6740495
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pyogenes
  ORGANISM  Streptococcus pyogenes
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 50024)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 50024)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-OCT-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-OCT-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001918058.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001918058.2
            Genome Coverage       :: 368x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/12/2023 11:23:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 1,806
            CDSs (total)                      :: 1,769
            Genes (coding)                    :: 1,722
            CDSs (with protein)               :: 1,722
            Genes (RNA)                       :: 37
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1, 1 (5S, 23S)
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 47
            CDSs (without protein)            :: 47
            Pseudo Genes (ambiguous residues) :: 0 of 47
            Pseudo Genes (frameshifted)       :: 30 of 47
            Pseudo Genes (incomplete)         :: 20 of 47
            Pseudo Genes (internal stop)      :: 9 of 47
            Pseudo Genes (multiple problems)  :: 11 of 47
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..50024
                     /organism="Streptococcus pyogenes"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN08555799-rid19682583.denovo.007"
                     /strain="MGAS11113"
                     /serotype="Emm28"
                     /isolation_source="normally sterile site"
                     /host="Homo sapiens"
                     /db_xref="taxon:1314"
                     /country="Finland"
                     /collection_date="2002"
                     /collected_by="National Public Health Laboratory"
     gene            110..1102
                     /locus_tag="VC122_000409"
     CDS             110..1102
                     /locus_tag="VC122_000409"
                     /EC_number="6.3.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016502595.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate--ammonia ligase"
                     /protein_id="HEP5640361.1"
                     /translation="MKKSFIHQQEEISFVKNTFTQYLIAKLDVVEVQGPILSRVGDGM
                     QDNLSGTENPVSVNVLKIPNATFEVVHSLAKWKRHTLARFGFNEGEGLVVNMKALRPD
                     EDSLDQTHSVYVDQWDWEKVIPDGKRNLAYLKETVETIYKVIRLTELAVEARYDIEAV
                     LPKKITFIHTEELVAKYPDLTPKERENAITKEFGAVFLIGIGGVLPDGKPHDGRAPDY
                     DDWTTETENGYHGLNGDILVWNDQLGSAFELSSMGIRVDEEALKRQVEMTGDQDRLAF
                     DWHKSLLNGLFPLTIGGGIGQSRMVMFLLRKKHIGEVQTSVWPQEVRDSYDNIL"
     gene            1225..1764
                     /gene="rsmD"
                     /locus_tag="VC122_000410"
     CDS             1225..1764
                     /gene="rsmD"
                     /locus_tag="VC122_000410"
                     /EC_number="2.1.1.171"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011018010.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (guanine(966)-N(2))-methyltransferase
                     RsmD"
                     /protein_id="HEP5640362.1"
                     /translation="MRVVSGEFGGRPLKTLDGKITRPTSDKVRGAIFNMIGPYFNGGR
                     VLDLFAGSGGLAIEAVSRGMSAAVLVEKNRKAQAIIQDNIIMTKAENRFTLLKMEAER
                     AIDCLTGRFDLVFLDPPYAKETIVATIEALAAKNLLSEQVMVVCETDKTVLLPKEIAT
                     LGIWKEKIYGISKVTVYVN"
     gene            1754..2245
                     /gene="coaD"
                     /locus_tag="VC122_000411"
     CDS             1754..2245
                     /gene="coaD"
                     /locus_tag="VC122_000411"
                     /EC_number="2.7.7.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020905327.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pantetheine-phosphate adenylyltransferase"
                     /protein_id="HEP5640363.1"
                     /translation="MLTKIGLYTGSFDPVTNGHLDIVKRASGLFDQIYVGIFDNPTKK
                     SYFKLEVRKAMLTQALADFTNVIVVTSHERLAIDVAKELRVTHLIRGLRNATDFEYEE
                     NLEYFNHLLAPNIETVYLISRNKWQALSSSRVRELIHFQSSLEGLVPQSVIAQVEKMN
                     EKT"
     gene            2232..3269
                     /locus_tag="VC122_000412"
     CDS             2232..3269
                     /locus_tag="VC122_000412"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002994811.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PDZ domain-containing protein"
                     /protein_id="HEP5640364.1"
                     /translation="MKRLKKIKWWLVGLLALISLLLALFFPLPYYIEMPGGAYDIRTV
                     LQVNGKEDKRKGAYQFVAVGISRASLAQLLYAWLTPFTEISTAEDTTGGYSDTDFLRI
                     NQFYMETSQNAAIYQALSLAGKPVTLDYKGVYVLDVNNESTFKGTLHLADTVTGVNGK
                     QFTSSAELIDYVSHLKLGDEVTVQFTSDNKPKKGVGRIIKLKNGKNGIGIALTDHTSV
                     NSEDTVIFSTKGVGGPSAGLMFTLDIYDQITKEDLRKGRTIAGTGTIGKDGEVGDIGG
                     AGLKVVAAAEAGADIFFVPNNPVDKEIKKVNPNAISNYEEAKRAAKRLKTKMKIVPVT
                     TVQEALVYLRK"
     gene            3684..4661
                     /locus_tag="VC122_000413"
     CDS             3684..4661
                     /locus_tag="VC122_000413"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002994809.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HEP5640365.1"
                     /translation="MVTIKQVAEEAGVSRSTVSRYISQKGYVSDDARHKIKAAIAKLH
                     YTPNVLAQSLKTKKNQLVGLLLPDISNPFFPRLARGAEEYLKEKGYRVMLGNISDSEA
                     LEEEYVHVLLQSNAAGIITTHDFTKRYPTLAIPVVVVDCVDQETQYGVFSDNRAGGLL
                     AAQTVWQAGAKEVLLIRGPLDNAENINERFEASFSYLQKQDVTMYVCDSQNFDFESIQ
                     LEASYNLKCYPTIDSIIAPSDIHAIAYIHELHSQGKKIPQDVQIIGYDDILMSQFIYP
                     SLSTIHQSSYLMGRYAAELVYTIASQLTVKANRIKLPVHYVERETIRKR"
     gene            5085..5729
                     /locus_tag="VC122_000414"
     CDS             5085..5729
                     /locus_tag="VC122_000414"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011018009.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1027 domain-containing protein"
                     /protein_id="HEP5640366.1"
                     /translation="MDFSERCQMKKEISPEMYNYNKFPGPKFIHFEEQVKAEGIDLLL
                     LEDVKNAFDTTSFGQRYTEVLLKYDYIVGDWGNEQLRLKGFYKDSDDIKKTNRISRLE
                     DYIKEFCNFGCAYFVLENLHPQDIKFEEERQPRRKKSPKSKSNRRKPNYSNQQPATPK
                     SKSKRVSKEKQPENQAFTSQKRRSNTKHKEKSKRNQTSQLNTKISHFIIRKKDK"
     gene            5733..6812
                     /gene="rlmN"
                     /locus_tag="VC122_000415"
     CDS             5733..6812
                     /gene="rlmN"
                     /locus_tag="VC122_000415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011018008.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (adenine(2503)-C(2))-methyltransferase
                     RlmN"
                     /protein_id="HEP5640367.1"
                     /translation="MKPSIYSLTRDELIAWAVERGQKQFRATQIWDWLYKKRVQSFEE
                     MTNISKDFVSILNDSFCVNPLKQRVVQESADGTVKYLFELPDGMLIETVLMRQHYGHS
                     VCVTTQVGCNIGCTFCASGLIKKQRDLNSGEITAQIMLVQKYFDDRKQGERVSHVVVM
                     GIGEPFDNYKNVMCFLRVINDDNGLAIGARHITVSTSGLAHKIRDFANEGVQVNLAVS
                     LHAPNNDLRSSIMRVNRSFPLEKLFSAIEYYIEKTNRRVTFEYIMLNEVNDSIKQAQE
                     LADLTKTIRKLSYVNLIPYNPVSEHDQYSRSPKERVLAFYDVLKKNGVNCVVRQEHGT
                     DIDAACGQLRSKTMKKDREKVTATK"
     gene            complement(6894..7520)
                     /locus_tag="VC122_000416"
     CDS             complement(6894..7520)
                     /locus_tag="VC122_000416"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010922497.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prepilin peptidase"
                     /protein_id="HEP5640368.1"
                     /translation="MIAFFSICSGILLTHLFYCLLLYFPDNGQEQLSKQTVIVCDQLT
                     FAKRVFRQLQNHFITSQKIFFSLEISLIIILWASMVHWVSASYCYLLLFSLLFSLFDW
                     RSQEYPFILWLFSFVSLLLFYSINYLSLILLLLGLLAHLRPFSIGAGDFFYLASLALV
                     LDLTSLIWLIQLASLAGITACLLLGIKRIPFIPYLSFGLFWIVLLEHF"
     gene            7748..8275
                     /locus_tag="VC122_000417"
     CDS             7748..8275
                     /locus_tag="VC122_000417"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012997888.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA starvation/stationary phase protection
                     protein"
                     /protein_id="HEP5640369.1"
                     /translation="MTNTLVENIYASVTHNISKKEASKNEKTKAVLNQAVADLSVAAS
                     IVHQVHWYMRGPGFLYLHPKMDELLDSLNANLDEMSERLITIGGAPYSTLAEFSKHSK
                     LDEAKGTYDKTVAQHLARLVEVYLYLSSLYQVGLDITDEEGDAGTNDLFTAAKTEAEK
                     TIWMLQAERGQGPAL"
     gene            8417..8620
                     /locus_tag="VC122_000418"
     CDS             8417..8620
                     /locus_tag="VC122_000418"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002983840.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YqgQ family protein"
                     /protein_id="HEP5640370.1"
                     /translation="MKTLYDVQQLLKNFGIFVYLGKRLYDIEMMKIELQRLYDSGLLD
                     KRDYLNAELILRREHRLELEKEG"
     gene            8625..9596
                     /locus_tag="VC122_000419"
     CDS             8625..9596
                     /locus_tag="VC122_000419"
                     /EC_number="2.7.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002995901.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ROK family glucokinase"
                     /protein_id="HEP5640371.1"
                     /translation="MSQKLLGIDLGGTTIKFGILTAAGEVQEKWAIETNILEGGKHIV
                     PDIIASIKHRLDLYGLSSADFVGIGMGSPGAVDRDTNTVTGAFNLNWKETQEVGSVVE
                     KELGIPFAIDNDANVAALGERWVGAGENNPDVVFMTLGTGVGGGIIADGNLIHGVAGA
                     GGEIGHMIVEPENGFACTCGSHGCLETVASATGVVKVARLLAEAYEGDSAIKAAIDNG
                     EGVTSKDIFMAAEAGDSFADSVVEKVGYYLGLASANISNILNPDSVVIGGGVSAAGEF
                     LRSRIEKYFVTFTFPQVRYSTKIKIAELGNDAGIIGAASLARQFITK"
     gene            9606..9995
                     /locus_tag="VC122_000420"
     CDS             9606..9995
                     /locus_tag="VC122_000420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002989081.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhodanese-like domain-containing protein"
                     /protein_id="HEP5640372.1"
                     /translation="MSPITLILWLLLVGIVGYYTWNYFSFRKMAKQVDNETFKDVMRQ
                     GQLIDLREPAAFRAKHILGARNFPAQQFDAAIKGLRKDKPVLIYENMRPQYRVPAVKK
                     LKKAGFEDVYVLKDGIDYWDGKVKQTT"
     gene            10178..12019
                     /gene="typA"
                     /locus_tag="VC122_000421"
     CDS             10178..12019
                     /gene="typA"
                     /locus_tag="VC122_000421"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012678311.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="translational GTPase TypA"
                     /protein_id="HEP5640373.1"
                     /translation="MTNLRNDIRNVAIIAHVDHGKTTLVDELLKQSHTLDERKELQER
                     AMDSNDLEKERGITILAKNTAVAYNDVRINIMDTPGHADFGGEVERIMKMVDGVVLVV
                     DAYEGTMPQTRFVLKKALEQNLIPIVVVNKIDKPSARPAEVVDEVLELFIELGADDEQ
                     LEFPVVYASAINGTSSLSDDPADQEHTMAPIFDTIIDHIPAPVDNSDEPLQFQVSLLD
                     YNDFVGRIGIGRVFRGTVKVGDQVTLSKLDGTTKNFRVTKLFGFFGLERREIQEAKAG
                     DLIAVSGMEDIFVGETITPTDCVEALPILRIDEPTLQMTFLVNNSPFAGREGKWITSR
                     KVEERLLAELQTDVSLRVDPTDSPDKWTVSGRGELHLSILIETMRREGYELQVSRPEV
                     IIKEIDGVKCEPFERVQIDTPEEYQGAIIQSLSERKGDMLDMQMVGNGQTRLIFLIPA
                     RGLIGYSTEFLSMTRGYGIMNHTFDQYLPVVQGEIGGRHRGALVSIENGKATTYSIMR
                     IEERGTIFVNPGTEVYEGMIVGENSRDNDLGVNITTAKQMTNVRSATKDQTAVIKTPR
                     ILTLEESLEFLNDDEYMEVTPESIRLRKQILNKAARDKANKKKKSAE"
     gene            12141..12392
                     /locus_tag="VC122_000422"
     CDS             12141..12392
                     /locus_tag="VC122_000422"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002983849.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3165 family protein"
                     /protein_id="HEP5640374.1"
                     /translation="MFYLIMAVSIISYYLYMAPKSVRNTLGMIGLVGLVALLIVLAGL
                     SFIKIMQTPPEFFIGMGMVALGYFALKDVRKMTKKPRVK"
     gene            12748..14106
                     /locus_tag="VC122_000423"
     CDS             12748..14106
                     /locus_tag="VC122_000423"
                     /EC_number="6.3.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011054713.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramoyl-L-alanine--D-glutamate
                     ligase"
                     /protein_id="HEP5640375.1"
                     /translation="MKVISNFQNKKILILGLAKSGEAAAKLLTKLGALVTVNDSKPFD
                     QNPAAQALLEEGIKVICGSHPVELLDEDFEYMVKNPGIPYDNPMVKRALAKEIPILTE
                     VELAYFVSEAPIIGITGSNGKTTTTTMIADVLNAGGQSALLSGNIGYPASKVVQKAIA
                     GDTLVMELSSFQLVGVNAFRPHIAVITNLMPTHLDYHGSFEDYVAAKWMIQAQMTESD
                     YLILNANQEISATLAKTTKATVIPFSTQKVVDGAYLKDGILYFKEQAIIAATDLGVPG
                     SHNIENALATIAVAKLSGIADDIIAQCLSHFGGVKHRLQRVGQIKDITFYNDSKSTNI
                     LATQKALSGFDNSRLILIAGGLDRGNEFDDLVPDLLGLKQMIILGESAERMKRAANKA
                     EVSYLEARNVAEATELAFKLAQTGDTILLSPANASWDMYPNFEVRGDEFLATFDCLRG
                     DA"
     gene            14106..15188
                     /locus_tag="VC122_000424"
     CDS             14106..15188
                     /locus_tag="VC122_000424"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003044364.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylglucosamine--N-acetylmuramyl-
                     (pentapeptide) pyrophosphoryl-undecaprenol
                     N-acetylglucosamine transferase"
                     /protein_id="HEP5640376.1"
                     /translation="MPKKILFTGGGTVGHVTLNLILIPKFIKDGWEVHYIGDKNGIEH
                     TEIEKSGLDVTFHAIATGKLRRYFSWQNLADVFKVALGLLQSLFIVAKLRPQALFSKG
                     GFVSVPPVVAAKLLGKPVFIHESDRSMGLANKIAYKFATTMYTTFEQEDQLSKVKHLG
                     AVTKVFKDANQMPESTQLEAVKEYFSRDLKTLLFIGGSAGAHVFNQFISDHPELKQRY
                     NIINITGDPHLNELSSHLYRVDYVTDLYQPLMAMADLVVTRGGSNTLFELLAMAKLHL
                     IVPLGKEASRGDQLENATYFEKRGYAKQLQEPDLTLHNFDQAMADLFEHQADYEATML
                     ATKEIQSPDFFYDLLRADISSAIKEK"
     gene            15189..16337
                     /locus_tag="VC122_000425"
     CDS             15189..16337
                     /locus_tag="VC122_000425"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010922494.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsQ/DivIB"
                     /protein_id="HEP5640377.1"
                     /translation="MAKDKEKQSDDKLVLTEWQKRNIEFLKKKKQQAEEEKKLKEKLL
                     SDKKAQQQAQNASEAVELKTDEKTDSQEIESETTSKPKKPKKVRQPKEKSATQIAFQK
                     SLPVLLGALLLMAVSIFMITPYSKKKEFSVRGNHQTNLDELIKASKVKASDYWLTLLT
                     SPGQYERPILRTIPWVKSLHLSYQFPNHFLFNVIEFEIIAYAQVENGFQPILENGKRV
                     DKVRASELPKSFLILNLKDEKAIQQLVKQLTTLPKKLVKNIKSVSLANSKTTADLLLI
                     EMYDGNVVRVPQSQLTLKLPYYQKLKKNLENDSIVDMEVGIYTTTQEIENQPEVPLTP
                     EQNAADKEGDKPGEHQEQTDNDSETPANQSSPQQAPPSPETVLEQAHG"
     gene            16549..17913
                     /gene="ftsA"
                     /locus_tag="VC122_000426"
     CDS             16549..17913
                     /gene="ftsA"
                     /locus_tag="VC122_000426"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002983860.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsA"
                     /protein_id="HEP5640378.1"
                     /translation="MARNGFFTGLDIGTSSIKVLVAEFISGEMNVIGVSNVPSTGVKD
                     GIIIDIEAAATAIKTAVEQAEEKAGMTIEKVNVGLPANLLQIEPTQGMIPVPSESKEI
                     KDEDVDSVVKSALTKSITPEREVISLVPEEFIVDGFQGIRDPRGMMGIRLEMRGLIYT
                     GPSTILHNLRKTVERAGIKVENIIISPLAMAKTILNEGEREFGATVIDMGGGQTTVAS
                     MRAQELQYTNIYAEGGEYITKDISKVLKTSLAIAEALKFNFGQAEISEASITETVKVD
                     VVGSEEPVEVTERYLSEIISARIRHILDRVKQDLERGRLLDLPGGIVLIGGGAIMPGV
                     VEIAQEIFGVTVKLHVPNQVGIRNPMFSNVISLVEYVGMMSEVDVLAQTAVSGEELLR
                     RKPIDFSGQESYLPDYDDSRRPESTIGYEQQASQTAYDSQVPSDPKQKISERVRGIFG
                     SMFD"
     gene            17937..19256
                     /gene="ftsZ"
                     /locus_tag="VC122_000427"
     CDS             17937..19256
                     /gene="ftsZ"
                     /locus_tag="VC122_000427"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003062681.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsZ"
                     /protein_id="HEP5640379.1"
                     /translation="MAFSFDTASIQGAIIKVIGVGGGGGNAINRMIDEGVAGVEFIAA
                     NTDIQALSSSKAETVIQLGPKLTRGLGAGGQPEVGRKAAEESEEILTEALTGADMVFI
                     TAGMGGGSGTGAAPVIARIAKSLGALTVAVVTRPFGFEGNKRGNFAIEGIEELREQVD
                     TLLIISNNNLLEIVDKKTPLLEALSEADNVLRQGVQGITDLITSPGLINLDFADVKTV
                     MANKGNALMGIGIGSGEERIVEAARKAIYSPLLETTIDGAQDVIVNVTGGLDMTLTEA
                     EEASEIVGQAAGQGVNIWLGTSIDDTMKDDIRVTVVATGVRQEKAEQVSGFRQPRTFT
                     QTNAQQVAGAQYASDQAKQSVQPGFDRRSNFDFDMGESREIPSAQKVISNHNQNQGSA
                     FGNWDLRRDNISRPTEGELDNHLNMSTFSANDDSDDELETPPFFKNR"
     gene            19259..19930
                     /locus_tag="VC122_000428"
     CDS             19259..19930
                     /locus_tag="VC122_000428"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009880395.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YggS family pyridoxal phosphate-dependent
                     enzyme"
                     /protein_id="HEP5640380.1"
                     /translation="MDLLTNKKKIFETIRLSTEAANRTNDSVSVIAVTKYVDSTIAGQ
                     LIEAGIEHIAENRVDKFLEKYDALKYMPVKWHLIGTLQRRKVKEVINYVDYFHALDSV
                     RLALEINKRADHPVKCFLQVNISKEESKHGFNISEIDEAIEEIGKMEKIQLVGLMTMA
                     PANASKESIITIFRQANQLRKNLQLKKRKNMPFTELSMGMSNDYPIAIQEGSTFIRIG
                     RAFFH"
     gene            19942..20598
                     /locus_tag="VC122_000429"
     CDS             19942..20598
                     /locus_tag="VC122_000429"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011018004.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein SepF"
                     /protein_id="HEP5640381.1"
                     /translation="MAFKDTFNKMISYFDTDEVNEVEEDVAASTDNVIPRSQQSVRAS
                     SHPKQEPRNNHVQQDHQARSQEQTRSQMHPKHGTSERYYQQSQPKEGHEMVDRRKRMS
                     TSGIANRREQYQQSTCSDQTTIALKYPRKYEDAQEIVDLLIVNECVLIDFQFMLDAQA
                     RRCLDFIDGASKVLYGSLQKVGSSMYLLAPSNVSVNIEEMTIPHTTQDIGFDFDMKRR
                     "
     gene            20603..20857
                     /locus_tag="VC122_000430"
     CDS             20603..20857
                     /locus_tag="VC122_000430"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003051849.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YggT family protein"
                     /protein_id="HEP5640382.1"
                     /translation="MILILSILLRLIKVYTYLLIAYALMSWFPGAYDSKIGRLISGIV
                     EPILKPFRAFNLQFAGLDFTIFVVIISLNFLAQVLVRVFI"
     gene            20857..21648
                     /locus_tag="VC122_000431"
     CDS             20857..21648
                     /locus_tag="VC122_000431"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011888737.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA-binding protein"
                     /protein_id="HEP5640383.1"
                     /translation="MVSHSKIYQHFHQEEYPFIDRMSDMINRVGDYYLLEVTEFLNPR
                     EVMILKSLIALTDLKMFVSTDYYPSEYGRVIIAPGYYDLEQSDFQIALVEISYQAKFN
                     QLTHSQILGTLINELGVKRNLFGDVFVEMGYAQLMIKRELLDYFLGTITKIAKTSVKL
                     REVNFDQLIRSIDNSQTLDILVSSFRLDGVVATILKKSRTQVIALIEANKIKVNYRLA
                     NKASDNLVIGDMVSIRGHGRFTLLADNGVTKHGKQKITLSKMIHK"
     gene            21658..22416
                     /locus_tag="VC122_000432"
     CDS             21658..22416
                     /locus_tag="VC122_000432"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011054711.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DivIVA domain-containing protein"
                     /protein_id="HEP5640384.1"
                     /translation="MALTTLEIKDKTFKTKFRGYCEEEVNEFLDIVVDDYEALVRKNR
                     DNEARIKDLEEKLSYFDEMKESLSQSVILAQETAEKVKATANAEATNLVSKATYDAQH
                     LLDESKAKANQMLRDATDEAKRVAIETEELKRQTRVFHQRLISSIESQLSLSNSPEWD
                     ELLQPTAIYLQNSDDAFKEVVKTVLNEDIPESDDSASFDATRQFTPEELEELQRRVDE
                     SNKELEAYQLDSQSDSTTEPEVNLSETQTFKLNI"
     gene            22689..25490
                     /locus_tag="VC122_000433"
     CDS             22689..25490
                     /locus_tag="VC122_000433"
                     /EC_number="6.1.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011285636.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="isoleucine--tRNA ligase"
                     /protein_id="HEP5640385.1"
                     /translation="MKLKETLNLGKTAFPMRAGLPNKEPQWQAAWEQAELYKKRQELN
                     AGKPAFHLHDGPPYANGNIHVGHALNKISKDIIVRSKSMSGFQAPYVPGWDTHGLPIE
                     QVLAKQGIKRKEMDLAEYLEMCRQYALSQVDKQRDDFKRLGVSADWENPYVTLDPQFE
                     ADQIRVFGAMAEKGYIYRGAKPVYWSWSSESALAEAEIEYHDIDSTSLYYANKVKDGK
                     GILDTNTYIVVWTTTPFTVTASRGLTVGPDMDYLVVKPAGSDRQYVVAEGLLDSLAGK
                     FGWESFETLASHKGADLEYIVTEHPWDTDVEELVILGDHVTLESGTGIVHTAPGFGED
                     DYNVGTKYKLEVAVTVDERGLMTENAGPDFHGQFYNKVTPIVIDKLGDLLLAQEVINH
                     SYPFDWRTKKPIIWRAVPQWFASVSDFRQDILDEIEKTTFHPSWGETRLYNMIRDRGD
                     WVISRQRAWGVPLPIFYAEDGTAIMTKEVTDHVADLFQENGSIIWWQKEAKDLLPEGF
                     THPGSPNGEFTKETDIMDVWFDSGSSWNGVMNTKENLSYPADLYLEGSDQYRGWFNSS
                     LITSVAVNGHAPYKAILSQGFVLDGKGEKMSKSKGNIISPNDVAKQYGADILRLWVAS
                     VDTDNDVRVSMEILGQVSETYRKIRNTLRFLIANTSDFNPATDTVAYADLGAVDKYMT
                     IVFNQLVATITDAYERYDFMAIYKAVVNFVTVDLSAFYLDFAKDVVYIEAANSLERRR
                     MQTVFYDILVKITKLLTPILPHTTEEIWSYLEHESEAFVQLAEMPVAETFSAQEDILE
                     AWSAFMTLRTQAQKALEEARNAKIIGKSLEAHLTIYASEEVKTLLTALDSDIALLLIV
                     SQLTIADLADAPADAVAFEGIAFMVEHAIGEVCERSRRIDPTTRMRSYNAFVCDHSAK
                     IIEENFPEAVAEGFEESDK"
     gene            complement(25755..26057)
                     /locus_tag="VC122_000434"
     CDS             complement(25755..26057)
                     /locus_tag="VC122_000434"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012678298.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1827 family protein"
                     /protein_id="HEP5640386.1"
                     /translation="MRLINTTSSHPELIRNQLKNTDAYLVEVYSAGNTDVIFTQAPKH
                     YELLISNKYRAIKEDELDIIREFFLKRKIDPKIVIPGQSKTLHTNNLIEISFQTSV"
     gene            complement(26108..26563)
                     /locus_tag="VC122_000435"
     CDS             complement(26108..26563)
                     /locus_tag="VC122_000435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002989103.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX hydrolase"
                     /protein_id="HEP5640387.1"
                     /translation="MMIPTFGHKNAHKDYVTRYGVYAIIPNHEQTKIILVQAPNGSWF
                     LPGGEIEAGEGQLQALERELIEELGFSATIGSYYGQADEYFYSRHRDTHFYHPAYLYE
                     VTAFQAVSKPLEDFNNLSWFSPIEAIAKLKRESHQWGVKEWQKKHHSTN"
     gene            complement(26691..28973)
                     /locus_tag="VC122_000436"
     CDS             complement(26691..28973)
                     /locus_tag="VC122_000436"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012560820.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent Clp protease ATP-binding subunit"
                     /protein_id="HEP5640388.1"
                     /translation="MLCQNCNLNESTIHLYTSVNGKQRQVDLCQNCYQIMKSDPANSI
                     LNGLTPGYRAQDRSTSPFFDDFFGDLNNFRAFGNLPNTPPTQAGQNGNGGGRYGGNYN
                     GQRPAQPQTPNQQAKGLLEEFGINVTDIARNGNIDPVIGRDEEITRVIEILNRRTKNN
                     PVLIGEPGVGKTAVVEGLAQKIIDGTVPQKLQGKQVIRLDVVSLVQGTGIRGQFEERM
                     QKLMEEIRNRKDVILFIDEIHEIVGAGSAGDGNMDAGNILKPALARGELQLVGATTLN
                     EYRIIEKDAALERRMQPVKVDEPSVEETITILKGIQPKYEDYHHVKYSPAAIEAAAHL
                     SNRYIQDRFLPDKAIDLLDEAGSKMNLTLNFVDPKEIDKRLIEAENLKAQATRDEDYE
                     RAAYFRDQITKYKEMQAQKVDEQDIPIITEKTIEAIVEQKTNIPVGDLKEKEQSQLVN
                     LANDLKAHVIGQDDAVDKIAKAIRRNRVGLGTPNRPIGSFLFVGPTGVGKTELSKQLA
                     IELFGSTNNMIRFDMSEYMEKHAVAKLVGAPPGYVGYEEAGQLTEQVRRNPYSLILLD
                     EVEKAHPDVMHMFLQVLDDGRLTDGQGRTVSFKDTIIIMTSNAGTGKSEASVGFGAAR
                     EGRTSSVLGELSNFFSPEFMNRFDGIIEFKALSKEHLLHIVDLMLEDVNERLGYNGIH
                     LDVTQKVKEKLVDLGYDPKMGARPLRRTIQDYIEDAITDYYLEHPTEKQLRALMTNSE
                     NITIKAVKEGDSFLNEESLD"
     gene            29271..29501
                     /locus_tag="VC122_000437"
     CDS             29271..29501
                     /locus_tag="VC122_000437"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003105222.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YkuJ family protein"
                     /protein_id="HEP5640389.1"
                     /translation="MESHLVRIINRLELMVTDGGNLKRNFERDGVVVAEVAFSNDPEN
                     GPVFTLRDVEARESYSFDSIDLIAMEIYDLLY"
     gene            29628..30314
                     /locus_tag="VC122_000438"
     CDS             29628..30314
                     /locus_tag="VC122_000438"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011018000.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter permease"
                     /protein_id="HEP5640390.1"
                     /translation="MDLSFLPKYWAYFNYGVLVTIMISVSVVFFGTLIGVLVTLIKRS
                     HVKPLTWVVNLYVWIFRGTPMVVQIMIAFAWMHFNNMPTIGFGVLDLDFSRLLPGIII
                     ISLNSGAYISEIVRAGIEAVPKGQLEAAYSLGIRPQNAMRYVILPQAFKNILPALGNE
                     FITIIKDSAFLQTIGVMELWNGAQSVVTATYSPISPLLVAAFYYLMVTTVMAQLLAVL
                     ERHMAQGGNH"
     gene            30314..31048
                     /locus_tag="VC122_000439"
     CDS             30314..31048
                     /locus_tag="VC122_000439"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014407690.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter ATP-binding protein"
                     /protein_id="HEP5640391.1"
                     /translation="MTEAIISIKDLHKYYGHNEVLKGIDLDIMPGEVVVIIGPSGSGK
                     STLLRTMNLLEVPTKGQIRFEGIDITDKKNDIFSMREKMGMVFQQFNLFPNMTVLENI
                     TLSPIKTKGMAKAEADKTALSLLDKVGLSEKAKAYPASLSGGQQQRIAIARGLAMDPD
                     VLLFDEPTSALDPEMVGEVLAVMQDLAKSGMTMVIVTHEMGFAKEVADRVIFMDGGVI
                     VEEGSPNQLFDLTKEERTKDFLSRVL"
     gene            31197..32606
                     /locus_tag="VC122_000440"
     CDS             31197..32606
                     /locus_tag="VC122_000440"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002989109.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="deoxyribodipyrimidine photo-lyase"
                     /protein_id="HEP5640392.1"
                     /translation="MVSVMWFRRDLRIDDQKALAHAIASQSPTLCVFQFNKEQLNLLH
                     SRNQSSFITSVLAFRKLLKCESIDLYLMYGDLMTCFEQLLTQLKDWTDVYFNYDESGY
                     GRLRDQKAAQFFKKNGIAVHTYQDHYLHGSQEIINQSGQPYKVFTPYYRIWQNYPKET
                     PIKVELSQGRWLNLETPDDVLRTVESFKDEKYQDVATFDEASKQLNRFIQDQLAAYHA
                     NRDFPAQLGTSRLSPFLRIGAIGIRTVYHAVRQAPNSLGQATFLKELAWRDFYNMVYV
                     AYPDQKTQPIQKAFSQIEWVNNPDWFQLWKEGKTGYPIVDAAMLQLQKTGWMHNRLRM
                     IVASFLTKDLLCDWRLGEQYFQQQLIDYDAASNIGGWQWAASTGTDAVPYFRIFNPVT
                     QGKRFDPKGEFIKAYLPQLEHVPEKYLHEPWKMPKYLQESVSCIIGTDYPQPIVDHAK
                     QREQAIAKYEWAKEKAKIE"
     gene            32784..34478
                     /locus_tag="VC122_000441"
     CDS             32784..34478
                     /locus_tag="VC122_000441"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014635581.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phospho-sugar mutase"
                     /protein_id="HEP5640393.1"
                     /translation="MTYNEVYQEWLHNNDLSDDIKADLAAIKDNEAEIQDRFYKTLEF
                     GTAGLRGKLGAGTNRMNTYMVGKAAQALANTIIDHGPEAVKKGIAVSYDVRYQSRTFA
                     ELTCSIMAANGIKAYLYKGIRPTPMCSYAIRALGCISGVMITASHNPQAYNGYKAYWQ
                     EGSQILDDIADQIAQHMAALTQYEEIKQMPFEKALDSGLVTYIDESIEEAYKKEVLGL
                     TINDTDIDKSVRVVYTPLNGVGNLPVREVLRRRGFENVYVVPEQEMPDPDFTTVGYPN
                     PEVPKTFAYSEKLGKAVDADILIATDPDCDRVALEVKNAVGDYVFLNGNKIGALLSYY
                     IFSQRFDLGNLPANPVLVKSIVTGDLSRAIASHYGIETVETLTGFKNICGKANEYDVT
                     KQKNYLFGYEESIGFCYGTFVRDKDAVSASMMIVEMAAYYKKKGQNLLDVLQTIYATF
                     GYYNERQIALELEGIEGQKRIARIMEDFRQTPIASVAEMALDKTIDFIDGYQDFPKQN
                     CLKFYLDDGSWYALRPSGTEPKIKFYLYTIGQTQENSATKLDAIEAACRTKINQVN"
     gene            34686..35540
                     /locus_tag="VC122_000442"
     CDS             34686..35540
                     /locus_tag="VC122_000442"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010922486.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional methylenetetrahydrofolate
                     dehydrogenase/methenyltetrahydrofolate cyclohydrolase"
                     /protein_id="HEP5640394.1"
                     /translation="MTELIDGKALAQKMQQELAAKVNNLKQKKGIVPGLAVILVGDDP
                     ASQVYVRNKERAALTVGFKSETVRLSEFICQEELIAVIERYNADNTIHGILVQLPLPN
                     HINDKKIILAIDPKKDVDGFHPMNTGHLWSGRPLMVPCTPSGIMELLREYNVNLEGKH
                     AVIIGRSNIVGKPMAQLLLDKNATVTLTHSRTRQLEEVCRCADVLIVAIGQGHFITKQ
                     YIKDGAIVIDVGMNRDDNGKLIGDVAFDEVAEVAAKITPVPGGVGPMTIAMLLEQTYQ
                     SALRSTHK"
     gene            35693..37033
                     /locus_tag="VC122_000443"
     CDS             35693..37033
                     /locus_tag="VC122_000443"
                     /EC_number="3.1.11.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002989115.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease VII large subunit"
                     /protein_id="HEP5640395.1"
                     /translation="MADYLTVTHLTKYLKLKFDRDPYLERVYLTGQVSNFRKRPTHQY
                     FSLKDESAVIQATMWAGVYKKLGFDLEEGMKINVIGRVQLYEPSGSYSIVIEKAEPDG
                     IGALALQFEQLKKKLTAEGYFEQKHKQPLPQFVSKIGVITSPSGAVIRDIITTVSRRF
                     PGVEILLFPTKVQGDGAAQEVVANIRRANQREDLDLLIVGRGGGSIEDLWAFNEEIVV
                     QAIFESQLPVISSVGHETDTTLADFVADRRAATPTAAAELATPITKTDLMSWIVERQN
                     RSYQACLRRIKQRQEWVDKLSQSVIFRQPERLYDAYLQKIDRLSMTLMNTMKDRLSSA
                     KENKVQLDHSLANSQLQTKIERYQDRVATAKRLLMANMASQYDSQLARFEKAQDALLS
                     LDASRIIARGYAMIEKNQALVASVSQITKGDQLTIKMRDGQLDVEVKDVKNENI"
     gene            37011..37226
                     /locus_tag="VC122_000444"
     CDS             37011..37226
                     /locus_tag="VC122_000444"
                     /EC_number="3.1.11.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002983901.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease VII small subunit"
                     /protein_id="HEP5640396.1"
                     /translation="MSKTKTFEENLQDLETIVNKLENGDVPLEEAISEFQKGMLLSKE
                     LQKTLQAAEKTLVKVMQADGTEVDMDD"
     gene            37226..38098
                     /locus_tag="VC122_000445"
     CDS             37226..38098
                     /locus_tag="VC122_000445"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011888744.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polyprenyl synthetase family protein"
                     /protein_id="HEP5640397.1"
                     /translation="MDKLARIDEAIRRYYKTTSNGVSEELIDAILYSVDSGGKRIRPL
                     ILLEMIEGFGVSLQNAHFDLAAALEMIHTGSLIHDDLPAMDNDDYRRGRLTNHKQFGE
                     ATAILAGDSLFLDPFGLIAQAELNSEVKVALIQELSLASGTFGMVGGQMLDMKGENQA
                     LSLPQLSLIHLNKTGKLLTFPFKAAALVTEQAITVRQQLEQAGMLIGHAFQIRDDILD
                     VTASFEDLGKTPKKDLFAEKATYPSLLGLEASYQLLTESLDQALTIFQTLESDVGFKP
                     QIITKLIEGLRLNA"
     gene            38091..38918
                     /locus_tag="VC122_000446"
     CDS             38091..38918
                     /locus_tag="VC122_000446"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015017216.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TlyA family RNA methyltransferase"
                     /protein_id="HEP5640398.1"
                     /translation="MPKERVDVLAYKQGLFETREQAKRGVMAGLVVSVINGQRYDKPG
                     DKIDDGTELKLKGEKLKYVSRGGLKLEKGLHVFGVSVANQIGIDIGASTGGFTDVMLQ
                     DGAKLVYAVDVGTNQLVWKLRQDPRVRSMEQYNFRYAQPEDFNEGQPVLASIDVSFIS
                     LSLILPALHNVLSDQGQVIALIKPQFEAGREQIGKKGIVKDKQIHEKVIQKVMDFASG
                     YGFTVKGLDFSPIQGGHGNIEFLAHLAKSQTPETLAPHLIQEVVAKAHKEFEKHEKE"
     gene            38905..39375
                     /locus_tag="VC122_000447"
     CDS             38905..39375
                     /locus_tag="VC122_000447"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002983907.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arginine repressor"
                     /protein_id="HEP5640399.1"
                     /translation="MKKSERLELIKKMVLTHPIETQHDLLRLLAEHGLELTQATISRD
                     MNEIGIVKIPSGSGRYIYGLSQDSGKKIVQGPRSIKSTILAVSDKTKGLEQHLYLKVV
                     PGNSKLIKRYLLADFSKAIFSLIADDDSLLLIAKSPSEADMIRQEILLWMQGIT"
     gene            39397..41058
                     /gene="recN"
                     /locus_tag="VC122_000448"
     CDS             39397..41058
                     /gene="recN"
                     /locus_tag="VC122_000448"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003051804.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein RecN"
                     /protein_id="HEP5640400.1"
                     /translation="MLLEISIKNFAIIDEISLNFENGMTVLTGETGAGKSIIIDAMNM
                     MLGARASTEVIRRGANKAEIEGFFSVDATPELVACLESSGIAMEEELIIRRDIFANGR
                     SVSRINGQMVNLATLKQVGQFLVDIHGQHDQEELMRPQLHQQILDAFGDKAFEQLKEN
                     YQLIFDRYKSLRRQVIDKQKNEKEHKDRIDMLAFQIAEIEAAALSRGEDDRLNQERDR
                     LMNHKQIADTLTNAYVMLDNDDFSSLSNIRSSMNDLLSIEQFDSEYKGMSTSISEAYY
                     ILEEVSKQLSDTIDQLDFDGGRLQEIEFRLDTLNSLTRKYGGNVNDVLDYYDNIIKEY
                     QLLTGDDLSSGDLESELKALEKQLVAAATNLSERRHQLAEQLEAEIKAELKELYMEKA
                     DFKVHFTTSKFNRDGNESLEFYISTNPGEGFKPLVKVASGGELSRLMLAIKAAISRKE
                     DKTSIVFDEVDTGVSGRVAQAIAQKIYKIGRHGQVLAISHLPQVIAIADYQYFISKES
                     KEESTVSKVRLLTPEERVEEIASMIAGTDMTQAALTQARELLAKH"
     gene            41230..42180
                     /locus_tag="VC122_000449"
     CDS             41230..42180
                     /locus_tag="VC122_000449"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011285633.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LPXTG cell wall anchor domain-containing
                     protein"
                     /protein_id="HEP5640401.1"
                     /translation="MTSKKACLSSIIVLASLTCGNDTVSANHLSATGDKFDDCSTLVE
                     KDVAPKDELEMLAWSSSQTTDDADRDYEDFLDDDSFISQNETDKMFENLTDDRLLDEL
                     DELDELDELDELDEEDTIEPEQNVIMPSDDELFDLTDAVETRLTVSSAPHLEAELPKP
                     HLRSLSDTALRSGEIRGHLDNKLDALSVTATKLALTMAQKFDLTTHVYSIGESFSEVL
                     AAHYEDRKAESAFSKKKRFHLPIATPDVVIEELRRLVSSIGSSKEDVSVPYNRKLGMA
                     VAKRKIALPQTGEEFSYYPALLGLMILGLTPIMIPKKINN"
     gene            42396..43247
                     /locus_tag="VC122_000450"
     CDS             42396..43247
                     /locus_tag="VC122_000450"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012767275.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DegV family protein"
                     /protein_id="HEP5640402.1"
                     /translation="MEKYMGTIKIVTDSSITIEPELIKALDITVVPLSVMIDSKLYSD
                     NDLKEEGHFLSLMKASKSLPKTSQPPVGLFAETYENLVKKGVTDIVAIHLSPALSGTI
                     EASRQGAEIAEAPVTVLDSGFTDQAMKFQVVEAAKMAKAGASLNEILAAVQAIKSKTE
                     LYIGVSTLENLVKGGRIGRVTGVLSSLLNVKVVMALKNDELKTLVKGRGNKTFTKWLD
                     SYLAKNSHRPIAEIAISYAGEASLALTLKERIAAYYNHSISVLETGSIIQTHTGEGAF
                     AVMVRYE"
     gene            43240..44082
                     /locus_tag="VC122_000451"
     CDS             43240..44082
                     /locus_tag="VC122_000451"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014635578.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SGNH/GDSL hydrolase family protein"
                     /protein_id="HEP5640403.1"
                     /translation="MNNRHLFSGIFFFVISLCLAFLLLNIIIPKSNSRLKKSDFLTKE
                     QVAIQYVAIGDSLTEGVGDLTHQGGFVPLLTNDLSEYFKANVNHQNYGVSGDTSQQIL
                     DRMIKQKQIQLSLKKADIMTLTVGGNDVMAVIRKNLADLQVSSFRKPARQYQKRLRQI
                     IELARKDNKDLPIFILGIYNPFYLNFPELTDMQKVIDDWNTKTKEVVGEYDRVYFVPI
                     NDLLYKGINGQEGIVHSSGDQTTIVNDALFTGDHFHPNNTGYQIMSNAVMEKIKKHEK
                     KIKP"
     gene            44060..44647
                     /locus_tag="VC122_000452"
     CDS             44060..44647
                     /locus_tag="VC122_000452"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010922482.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YpmS family protein"
                     /protein_id="HEP5640404.1"
                     /translation="MKKKSNLNWWKWSFLCLLAFNTAFLMVIASRLIQVREPESELIA
                     KKPVKNIKIGTFVTTREQLNETVASYLKDYQTKKMSYKFYATSSSILFEGTYQLLGYE
                     VPLYIYFQPHRLENGAVQLQVISFSVGTLPLPEKDVLQYLKSSYKLPSFVKVMPNQSA
                     IVVNLQDIQNDAKVYLKAKKIDLFNDEISFNIYKK"
     gene            44746..45021
                     /locus_tag="VC122_000453"
     CDS             44746..45021
                     /locus_tag="VC122_000453"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002950996.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HU family DNA-binding protein"
                     /protein_id="HEP5640405.1"
                     /translation="MANKQDLIAKVAEATELTKKDSAAAVDAVFSTIEAFLAEGEKVQ
                     LIGFGNFEVRERAARKGRNPQTGAEIEIAASKVPAFKAGKALKDAVK"
     gene            45375..47237
                     /gene="cadA"
                     /locus_tag="VC122_000454"
     CDS             45375..47237
                     /gene="cadA"
                     /locus_tag="VC122_000454"
                     /EC_number="7.2.2.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011528775.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cadmium-translocating P-type ATPase"
                     /protein_id="HEP5640406.1"
                     /translation="MTIRQWMADHLHLMETLACLVLIIIGLAFLHSFPQVASAIFITA
                     FLIGGYASAKTGILDLVKNKHLSVDILMILAAIGAGIIGYWLEGALLIFIFSLSNTLE
                     EMAMEKSKDAISALMSLTPDTARQYQEDGHILEVETRSLSVGDRLQVRKGEAVPIDGQ
                     LLSPFGQFDESMVTGEPITVDKAEGQDLIGGTINQGQTIDMLVTIENDDTLFAKIINL
                     VESAQEKKSKTATFIESLEDGYVKFVLVLIPAFILFSHFVLSWTWLAAFYRGMILLTV
                     ASPCALIASSTPASLAAISRAARKGLIIKGGDIVDNMGDIKAVVMDKTGTLTQGKPSV
                     VNAHYLEDELLVNRLVKGAETASTHPISKALLEYTEKLEPLTFDHLEEISGKGFQGFY
                     QGQEWRIGKKTFILEKVQDLSAFEETIQVEENQGKTLIFVSRDHQLIAYYALLDDIKI
                     ESKRAIKSLHAMGIKTVMLTGDQERTANYVAQKLGIDEVVANCMPQDKVAKLAELKTK
                     YGFVAMVGDGINDAPALAQADVSYAIGSGTDIAMESADSVIMDDLTRIPFSIQLSRKM
                     KTIIKQNIVFALSVITLLILANVFQVVNLPLGVVGHEGSTILVILNGLRLLSFK"
     gene            complement(47537..48472)
                     /locus_tag="VC122_000455"
     CDS             complement(47537..48472)
                     /locus_tag="VC122_000455"
                     /EC_number="1.3.98.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004228450.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroorotate oxidase"
                     /protein_id="HEP5640407.1"
                     /translation="MVSTATQIGHFSFDNCLMNAAGVYCMTKEELMEVEKSQAASFVT
                     KTGTLEVRPGNPEPRYADTRLGSINSMGLPNNGFRYYLDFVSDLAKTGQHKPHFLSVV
                     GLSPTETETILKAIMASDYEGLVELNLSCPNVPGKPQIAYDFETTDQLLENIFTYYTK
                     PLGIKLPPYFDIVHFDQAAAIFNKYPLSFVNCVNSIGNGLVIEDEQVLIKPKNGFGGI
                     GGDYIKPTALANVHAFYKRLKPSIHIIGTGGVKTGRDAFEHILCGASMVQIGTALHQE
                     GPAIFERVTKELKTIMVEKGYQSLDDFRGNLRYKD"
     gene            48711..49406
                     /locus_tag="VC122_000456"
     CDS             48711..49406
                     /locus_tag="VC122_000456"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003051611.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoglycerate mutase"
                     /protein_id="HEP5640408.1"
                     /translation="MVKLVFARHGESEWNKANLFTGWADVDLSEKGTQQAIDAGKLIK
                     EAGIEFDLAFTSVLTRAIKTTNLALENAGQLWVPTEKSWRLNERHYGALTGKNKAEAA
                     EQFGDEQVHIWRRSYDVLPPAMAKDDEYSAHKDRRYADLDPALIPDAENLKVTLERAM
                     PYWEEKIAPALLDGKNVFVGAHGNSIRALVKHIKGLSDDEIMDVEIPNFPPLVFELDE
                     KLNIVKEYYLGGE"
     gene            49513..>50024
                     /locus_tag="VC122_000457"
     CDS             49513..>50024
                     /locus_tag="VC122_000457"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014407681.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription repressor NadR"
                     /protein_id="HEP5640409.1"
                     /translation="MKAEDRRQKIIECLNSEQKAVSATRLGKLLGVSRQVIVGDIALL
                     RAQQIDIISTPKGYIMSTALYSHQFCARIVCQHNVEETKKELEIILAHQGIITTVEVE
                     HPIYGMITAPLNIKTHSDVTNFMSKLSQSKAELLSSLTEGLHSHLISCPSQEAFLAIK
                     HDLELAGILY"
BASE COUNT        15950 a         8518 c        10506 g        15050 t
ORIGIN      
        1 ttatttttca aaatattaga ataaacaaaa gtggacaacg tataaagaga actgctaagt
       61 cgttgagttc attagtccca aaaagattac caaaaatgaa ggaggagcta tgaagaaaag
      121 ttttattcat caacaagagg agatttcctt tgttaagaat acgttcactc agtatttgat
      181 tgctaagtta gatgttgttg aagtgcaagg acccatttta agtcgggtcg gggacggtat
      241 gcaagataat ttgtccggta ctgaaaatcc agtatctgtc aatgtcttga aaattcctaa
      301 tgcaaccttt gaagttgtgc attcattagc aaaatggaaa cgtcacacct tagcccgttt
      361 tgggtttaat gaaggtgaag gccttgttgt taatatgaag gctctccgac ctgatgagga
      421 ttcacttgac caaacccatt ctgtctatgt tgaccaatgg gactgggaaa aagtcatccc
      481 tgatggcaaa cgtaatctcg cttacctcaa agaaaccgtt gaaaccattt acaaggtcat
      541 tcggttaact gaattagctg tagaagctcg ttatgatatt gaggctgttt tacctaagaa
      601 aataaccttc attcataccg aagaattggt agctaaatac cctgatttga cgcccaaaga
      661 acgtgaaaat gccattacta aagaatttgg agcagtcttt ttaattggta ttggcggggt
      721 tttacctgac ggtaaaccac acgatggccg cgctcctgac tatgatgact ggacaacaga
      781 aactgaaaat ggctatcatg ggcttaatgg tgatattttg gtttggaatg accaacttgg
      841 ttcagccttt gaattatcct caatggggat ccgtgtggat gaagaggccc taaaacgtca
      901 ggtggaaatg actggtgacc aagaccgctt agcctttgat tggcacaaat ctttgttgaa
      961 tggacttttc cctctaacga ttggtggagg aatcggtcaa tcacgtatgg tcatgttttt
     1021 gttgcgtaaa aaacatattg gagaagttca gacctctgtt tggccccaag aagttcgtga
     1081 tagctacgat aatattttat aagtaatcac atcattttgc taagttttcc acttgtataa
     1141 gagactttaa ataaatgatc agaaatattg aaaaaagaaa tcatttttaa ggtttagagc
     1201 tagaaatttg ctataataaa tattatgaga gttgtatcag gtgaatttgg tgggcgtcct
     1261 ttaaagacgc tcgatggaaa gataacacgc cctacttcag acaaagttag aggtgcgatt
     1321 tttaatatga taggacctta ctttaatggt ggtcgtgttt tagatttgtt tgcaggttca
     1381 ggtggattag ccattgaggc tgtttctcgt gggatgtctg ctgcagtttt ggttgagaag
     1441 aatcgtaaag cgcaagctat tattcaagac aatattataa tgactaaagc tgagaatcgg
     1501 tttaccttgt taaaaatgga agctgagcga gctattgatt gcttgactgg tcgatttgac
     1561 ttagtctttt tagaccctcc ttatgccaaa gaaacaattg tggcaactat tgaagcttta
     1621 gcagctaaaa acttattgag tgaacaggtt atggttgtct gtgagacaga taaaacagtc
     1681 ttgttgccaa aagagattgc aacgttaggt atttggaaag aaaaaattta tggaattagt
     1741 aaggtaacag tttatgttaa ctaaaatagg actttataca gggtcatttg atcctgttac
     1801 aaacggtcat ctcgatattg ttaaacgggc aagtggccta tttgatcaaa tatatgttgg
     1861 gatttttgat aatcctacca aaaaaagcta ttttaaacta gaagttcgta aagcaatgct
     1921 tactcaagct ctggctgatt ttacaaatgt aattgtagta acttcgcacg aacgtttagc
     1981 tattgatgta gctaaagaat tacgagtaac tcacttgatt agaggcttgc gaaatgcaac
     2041 tgactttgag tatgaagaaa atttggaata ttttaatcac cttttagctc caaatattga
     2101 gaccgtctat ttgatatcaa gaaataaatg gcaggccctt agctctagtc gtgtgagaga
     2161 gctaattcat tttcagtctt ccttagaagg tttggttcct cagtcagtta ttgctcaagt
     2221 ggagaaaatg aatgaaaaga cttaaaaaaa tcaaatggtg gttagtgggt ctgctagctt
     2281 taatctcttt gttgctagcg ttattttttc cgctacctta ttatattgaa atgcctggag
     2341 gcgcttacga tattcggact gtcttacaag tcaatggcaa agaagacaaa cgaaaaggag
     2401 cttaccagtt tgttgcagtg ggcattagtc gtgccagcct cgcgcagcta ttatatgctt
     2461 ggctgacacc gtttactgaa attagtacag cagaagatac aacaggcgga tacagcgata
     2521 ctgatttcct tcgaattaat caattttaca tggaaacatc acaaaatgca gctatttatc
     2581 aagctttatc cttagctgga aaaccagtta cattagatta taaaggcgta tatgttttag
     2641 acgtaaacaa cgaatctact tttaaaggaa cgttacactt agcagatact gtaacaggtg
     2701 taaatggtaa acagtttact agttcagcag aacttattga ctatgtttct cacctaaaac
     2761 taggggatga agttacggtt cagtttacga gtgataataa gcctaaaaaa ggagttggcc
     2821 gtattatcaa actgaaaaat gggaaaaatg ggattggcat tgccttgact gatcatacaa
     2881 gtgtcaattc agaagacaca gtgatcttta gtactaaagg agtaggagga cctagtgctg
     2941 gtctaatgtt tactcttgat atatatgatc aaataactaa agaagattta cgcaagggcc
     3001 gtacaattgc aggtacagga actattggca aggatggcga agtaggagat attggtggtg
     3061 caggtcttaa agtagttgca gcagctgaag ctggtgcaga tatatttttt gttccgaata
     3121 atcctgttga taaggaaatt aaaaaagtta atccaaatgc tataagtaat tacgaagaag
     3181 ccaaacgggc agccaaacga ctaaagacca aaatgaagat tgttcctgtt acgactgttc
     3241 aagaggcact ggtttatctt cgcaaataaa ataactatga atgtattata ttcaattata
     3301 ttcacgctat taactcataa aaaaacggtt gaaccagata gacagatcct tgaccagtct
     3361 tttgggttca atcatttttt acataaaaca tatctaactt gacagcgtaa aaaagatgtt
     3421 ataaaagtct agtggtaaaa aggcaataga gaattgagta aactttagtc gattatctgg
     3481 aaatagagca tactaaaaac acctagtgaa aattgatttt tttcactagc tcatccaaga
     3541 acttgcgcag ataatcatga gtccattcca tgactctatt attctggaaa gccttgcaat
     3601 agacaagcta acgttataac aactaaatta aaaacttgag attgaaaaat ccccataaac
     3661 ttattttaca aggaggtaag gctatggtaa ccattaaaca agttgctgaa gaagcaggtg
     3721 tttctagatc aacagtctcc aggtatattt ctcaaaaagg atatgtatct gatgatgcaa
     3781 ggcataaaat caaagcagcg atagcaaaat tacattacac tccaaatgtc ttggcgcagt
     3841 cattgaagac gaaaaaaaat caactagtcg gtttattatt accagacatt tccaatcctt
     3901 tttttccgag gttagcaaga ggagcagaag aatatttaaa agaaaagggg tatcgggtta
     3961 tgctaggaaa tatttcagac agcgaagctc ttgaagaaga atacgtccat gtccttcttc
     4021 aaagcaatgc tgctggcatt attactaccc atgactttac aaagcggtat cctactcttg
     4081 ctattccagt tgtagtggta gattgtgttg accaagagac ccagtacggt gtcttttcag
     4141 ataatagggc agggggctta ttggcggctc agactgtctg gcaagcaggg gcgaaagaag
     4201 ttctgttaat aagaggtccg ctggataatg ctgaaaacat taatgagcgg tttgaagcta
     4261 gtttttctta ccttcaaaaa caagacgtga ctatgtatgt gtgtgatagt cagaattttg
     4321 attttgaaag tattcagcta gaagctagtt acaacctcaa gtgttaccca acaatagata
     4381 gtattatagc accttctgat atccatgcta ttgcttatat tcatgaactg cattctcaag
     4441 gtaaaaagat tcctcaagat gttcaaataa taggttatga tgacatttta atgagtcaat
     4501 ttatttatcc ttctttatcg actattcatc aatcctctta tttgatgggc cgttatgccg
     4561 ccgaattagt ttatactatt gctagtcaat taactgtgaa agcaaatcgg attaaattgc
     4621 ctgttcacta cgttgagcga gaaacaataa ggaaaagata agtaataata ttagtccaga
     4681 ttttagcagg tatgatgaag atttattggg atttaccttc tactttaaaa gatgttatct
     4741 gtcattactt tcatcgctat cagtcaaaac gcattcttat gcaactctta accttaagct
     4801 taatgtcttt ttgataacat agaggaatgt tacacgatga tgacgcaata aagcctaaat
     4861 atagtaattg tcgtgatttt atagctagtg agtaatgaga tagcaagtct ctcatttact
     4921 tacaaaagtc aaaaaatagt gtataatggt tgagttacag ataacactgc aaaaaagata
     4981 acaagtgtcc ataattatct aagactataa aggcgatata gacagatata aaaaagatga
     5041 cgaatcagtt atcactggac aattaaccga tttagttaca actaatggat ttttcagaaa
     5101 ggtgtcaaat gaaaaaagaa atttcgccag aaatgtacaa ctataataaa tttccaggtc
     5161 ccaaatttat tcattttgaa gagcaagtta aggctgaagg cattgatttg ttactcttag
     5221 aagatgttaa gaacgctttt gatacgacta gctttggtca acgttataca gaagttctcc
     5281 taaagtatga ctatattgtt ggcgattggg gaaatgaaca gcttcgtctt aaagggtttt
     5341 ataaggatag tgatgatatc aagaaaacga atcgcatctc acgtttagaa gattatatta
     5401 aagaattttg caattttggt tgtgcttatt ttgttttaga aaatcttcat ccacaagata
     5461 ttaaatttga agaggagcgc caaccaagac gaaagaaatc acctaaatca aaatcaaatc
     5521 gtcgcaagcc aaactattca aatcagcagc ccgcaacacc taagagtaaa tcgaagcggg
     5581 tatcaaaaga aaagcaacct gaaaaccaag cgtttaccag tcaaaaacgt cgaagtaata
     5641 ctaagcataa agaaaagtca aaacgtaatc agactagtca acttaatacc aaaattagtc
     5701 attttatcat cagaaagaaa gataaataat ccatgaaacc atcgatttat agcttaacac
     5761 gcgatgaact gatcgcgtgg gcagttgaac gcggccaaaa acagtttaga gctactcaaa
     5821 tttgggattg gctctataaa aaaagagttc aatcttttga ggagatgacg aatatttcta
     5881 aagattttgt ttctatttta aatgatagtt tctgtgtcaa tcctttaaaa cagcgagttg
     5941 tacaagagtc agctgatggt acagtaaaat atttatttga actgcccgat ggtatgttaa
     6001 ttgaaacagt tttaatgcgt caacattatg gtcattctgt ttgtgtgacg actcaagttg
     6061 gctgtaacat tggctgtact ttctgtgcta gtggattgat caaaaagcaa cgtgatctaa
     6121 acagtgggga aattacagct caaatcatgt tagtccaaaa atattttgac gatagaaagc
     6181 aaggtgagcg ggttagccat gttgtcgtaa tgggcattgg tgaacctttt gataattaca
     6241 aaaatgtcat gtgctttctt cgcgtcatca acgatgataa tggcttagca attggtgccc
     6301 gtcatattac agtctctacc tcagggttag ctcacaagat tcgggatttt gcaaacgagg
     6361 gagtacaggt taatttagca gtctcgttgc atgcaccaaa taatgattta cgttcaagta
     6421 ttatgcgagt aaatcgctct tttccgctgg aaaagctctt ttcagctata gaatactata
     6481 ttgaaaaaac gaatcgtcgt gtcacttttg aatacattat gctaaatgaa gttaacgata
     6541 gcattaagca agctcaagaa ttagcagact taactaaaac tattcgtaag ttatcttatg
     6601 ttaacttgat tccgtataat cctgtttcag aacatgatca gtatagtaga agtcctaaag
     6661 agcgagttct agcgttttat gatgtgctaa agaaaaatgg agttaactgt gtggtgcgtc
     6721 aagagcacgg aacagatatt gatgcagcct gtggccagtt acgttctaaa acgatgaaaa
     6781 aagatcgcga aaaggttaca gcgacaaaat aaagcccaaa aaatatcttt gattgacaat
     6841 gcttgatgac aaaatattcc tagtcactaa aatgactagg ttttttatct ggcctaaaaa
     6901 tgctctagaa gcacaatcca aaataatcca aaactaagat aaggaataaa aggtatgcgc
     6961 ttgatgccta atagtaagca agcagtaata cctgctaaac tagcaagttg aattagccaa
     7021 atcaacgacg ttagatccaa tactaaagct aagctagcaa ggtagaaaaa atcgcctgct
     7081 ccaattgaaa aaggtcttaa atgagctaat aaacctagta gcagtaatat caatgataaa
     7141 taattgatcg aataaaacag gagtagactt acaaagctaa aaagccacaa gataaaaggg
     7201 tattcttgag aacgccaatc aaataaacta aataataaag aaaagagtaa caagtaacag
     7261 taactagcac taacccaatg taccatacta gcccataaga taattattag acttatctct
     7321 aagctaaaga agattttttg gctagttata aaatggtttt ggagctgccg aaaaacgcgt
     7381 ttggcaaaag ttagttggtc acaaacaatg acagtctgtt tacttagctg ttcttgacca
     7441 ttatcaggaa aataaagtag taaacagtaa aaaagatggg tcaataagat gcccgaacat
     7501 atactaaaaa aagctatcat acttatttat tcgaaaagaa atcaaatact tgcaatcttt
     7561 ccttaatcat tgtagaatat agatataaaa cgataatgat tctaattaag tgtctaatca
     7621 tccaattagg attattccgt tattgtcgaa taggattagg tccttaaccc tttcttatgt
     7681 ttatcacgtt agggttagtt ccccctccat tcttgccctt taagctgaaa agtaaggaga
     7741 tgttattatg acaaacacac tcgttgaaaa tatttatgct tcagtaactc acaatatttc
     7801 taaaaaagaa gcatcaaaaa atgaaaaaac taaagctgtt ttaaaccaag ctgtcgctga
     7861 cttatctgtg gctgcatcta ttgtgcacca agtacactgg tatatgcgtg gtccaggttt
     7921 cttgtacctt cacccaaaaa tggatgaatt acttgatagc ttgaatgcta atttagatga
     7981 aatgagcgag cgtttgatca ctattggcgg agcgccttat tctaccctag ctgagttttc
     8041 taaacactct aaattggacg aagcaaaagg tacttacgat aaaacagttg ctcagcattt
     8101 agcaagattg gtcgaagttt acctttacct cagcagcctt taccaagttg gtttggatat
     8161 taccgatgaa gaaggcgatg ctggaacaaa cgatttgttt acagcagcta agacagaggc
     8221 tgaaaaaacc atttggatgc tccaagcaga gcgtggacaa ggcccagcac tctaatggat
     8281 tatgccttag gataaaagaa gtcaatgata aaattggctt cttttttatg gaatcatcga
     8341 ttcattttca gaaacataga aagcgattcc atacttgtct tgaaatttgc cgtagaattt
     8401 gctaaaatct acttatatga aaacactata tgatgtgcaa caattgctaa aaaactttgg
     8461 catttttgtt taccttggca aacgtctcta tgatattgaa atgatgaaga ttgaacttca
     8521 gcgtctctat gatagcggct tattggataa gagggattac ttaaatgcgg agttaatttt
     8581 gcgacgtgag catagattag aattggaaaa agaaggatag acaaatgagt caaaaattac
     8641 tggggattga tttaggtggt accaccatta aatttggaat tttaacagca gcaggagaag
     8701 tccaagaaaa atgggcaatt gagactaata ttttagaagg aggcaaacat attgtccctg
     8761 atatcatcgc ttccatcaaa catcgcttag acttgtatgg tctaagcagt gctgattttg
     8821 tgggaattgg tatgggatca ccaggagcgg ttgaccgtga tactaataca gttactgggg
     8881 cattcaacct taattggaaa gaaacccaag aagtcggttc cgttgttgaa aaagaattgg
     8941 gcattccttt tgccattgac aatgatgcca atgtggctgc ccttggtgaa cgttgggtag
     9001 gtgctggtga aaataaccca gatgtcgtct tcatgacact tggaacaggt gtcggtggag
     9061 gcattattgc tgatggtaac ttgattcatg gtgttgcagg agcaggtggt gaaatcggcc
     9121 acatgattgt tgagccagaa aatggctttg cttgtacttg tggctcacac ggctgtttgg
     9181 aaacagtagc ttcagcaaca ggagttgtca aagtggcacg tttattggca gaagcttatg
     9241 aaggggattc agccatcaaa gcagctattg acaatggtga aggtgttacc agcaaagata
     9301 tttttatggc ggctgaagca ggggattcct ttgctgattc tgttgtggaa aaggttggtt
     9361 actaccttgg ccttgcttca gcaaatattt ccaatatctt gaatccagac tcagtggtta
     9421 tcggtggggg tgtgtcggca gcaggagaat ttctacgctc acgtattgaa aaatactttg
     9481 tgaccttcac tttcccacaa gtgaggtatt caactaaaat taagattgca gagcttggca
     9541 atgatgctgg aattatcggt gcagctagcc ttgctcgaca atttatcaca aaataggagg
     9601 gggatatgtc tccaatcaca cttatcttat ggttacttct tgtcggcatt gttggttatt
     9661 atacatggaa ctatttctct tttcgaaaaa tggcaaagca ggttgataac gagactttta
     9721 aagacgtgat gcgtcaaggt caattgattg atttacgaga gccagcggct tttagagcta
     9781 agcatatttt aggagctcga aattttccag cgcaacaatt tgatgctgct atcaaaggtc
     9841 ttcgcaagga caaacctgtt cttatttacg aaaatatgcg tccccagtac cgtgtgcctg
     9901 cggtcaaaaa acttaaaaaa gctggctttg aagacgttta tgtcttaaaa gacggtattg
     9961 attattggga tggcaaagtt aaacaaacaa cctagttaag agtaacgtgg agttaagggg
    10021 aataaaggta gtcactgtct caaaaacctt aaatcctttt tttgctgtat ccagacttgc
    10081 tgaaagtctg aaaacattta caattgatta aaaccagttt tttaaaacat tttgtgttat
    10141 acttatctag ttaaaatata tttacttaga ggaacaaatg actaacttaa gaaacgatat
    10201 ccgtaacgta gcgattattg cccacgttga ccacggaaaa acaacacttg tagatgaatt
    10261 attaaaacaa tcccatactc ttgatgagcg taaagagctt caagagcgtg ccatggattc
    10321 caatgacctt gaaaaagaac gtgggattac aatccttgcg aaaaatacgg cagtagccta
    10381 taacgatgtt cgtattaaca tcatggatac cccaggacac gcggacttcg gtggtgaagt
    10441 tgaacgtatc atgaaaatgg ttgacggggt tgttcttgtt gtggatgcct acgaaggaac
    10501 aatgccccag acgcgtttcg tattgaaaaa agcacttgag caaaacctta tcccgatcgt
    10561 tgtggtgaac aagattgaca aaccttcagc tcgtccagca gaagttgtag atgaagtgct
    10621 tgaattattc atcgaacttg gtgccgatga tgagcaattg gaattcccag ttgtttacgc
    10681 atcagctatt aatggaacat catcattatc agatgaccct gctgaccaag agcatactat
    10741 ggcaccgatc tttgatacga ttattgatca tattccagcg ccagttgata attcagatga
    10801 gcctttgcaa ttccaagtgt cacttttgga ctacaacgat ttcgtaggtc gtatcggtat
    10861 cggtcgtgtt ttccgtggta ctgttaaagt gggtgaccaa gtaactcttt caaaacttga
    10921 tggtaccact aaaaacttcc gtgttacaaa actgtttggt ttcttcggtt tggaacgtcg
    10981 tgaaattcaa gaagctaaag caggtgactt gattgctgtt tcaggtatgg aagatatctt
    11041 tgttggagaa accattacac caactgactg tgtggaagct ctgccaattc ttcgtattga
    11101 tgagccaaca cttcagatga ctttcttggt caataactct ccttttgcag gtcgtgaagg
    11161 taaatggatc acgtcacgta aggttgaaga acgtctttta gcagaattgc aaacagacgt
    11221 gtcacttcgt gttgacccaa cagattcgcc agataaatgg acggtttcag ggcgtggaga
    11281 attgcattta tctatcctca ttgaaaccat gcgccgtgaa ggctatgaac ttcaagtatc
    11341 acgtccagaa gttatcatca aagaaattga tggtgtcaaa tgtgaaccgt ttgagcgtgt
    11401 tcaaattgat acaccagaag aatatcaggg tgcaatcatc cagtctcttt cagaacgtaa
    11461 aggggatatg cttgatatgc agatggttgg taatggtcaa acgcgtttga ttttcttgat
    11521 tcctgcacgt ggtttgattg gttattcaac agagtttctt tcaatgacac gtggatatgg
    11581 tatcatgaat catacttttg atcagtatct accggttgtt caaggagaaa tcggtggccg
    11641 tcatcgtggt gccttggttt ctattgaaaa tgggaaagca acgacatatt caatcatgcg
    11701 tattgaagaa cgtggaacta tctttgtcaa cccaggtaca gaagtttacg aagggatgat
    11761 tgttggtgaa aactcacgtg ataacgacct tggtgttaac attacaacag ctaagcaaat
    11821 gacaaatgtc cgttcagcga ctaaagatca aactgcagtt atcaagacgc ctcgtatcct
    11881 aacgcttgaa gaatcattag agttcttgaa tgacgacgaa tacatggaag taacgccaga
    11941 atctatccgc ttgcgtaaac aaatcttgaa taaagctgcg cgtgataaag ctaataaaaa
    12001 gaaaaaatca gccgaatagc ttttgaagtt aaactaacta aataaaatag caaaaagata
    12061 tttaagaaaa gtcatttttt aaaggtcttt ttgcgtttac aagttcctat ggaatataaa
    12121 ccaaaataaa ggaggacgga atgttttacc ttattatggc tgtctcgatt atatcttatt
    12181 atctatacat ggctcctaaa agtgtccgta acacccttgg gatgattggt ttagttggct
    12241 tggttgctct gcttattgtt ttagcgggct tgagttttat caaaatcatg caaacccctc
    12301 ctgaattttt tattgggatg ggaatggtgg cattgggcta ttttgcttta aaagatgtgc
    12361 gtaaaatgac caaaaaaccg agagttaaat aaaaatactt aaagattgat gattttggtt
    12421 tttatacgat aaaaggactg atttgtttta gacaatatca gtcctttttt aatagttcat
    12481 tttttctgaa agaaagcttt tagccgattt tcattgagtg tgcaactaaa aaaagatgct
    12541 tggtagtgac agttaagacc tctgactagt attttatctt actttactta aaaaaacgta
    12601 ccaataatct cacaaatatg ggcatgatgt ttgtatattg gaaacggatt ctaattctaa
    12661 actcaagaac ggtattttta aaatgttgaa aaagcatcta aataaaggta aaatagaaat
    12721 caaatagaat ggatagtgtg agcttagatg aaagtgataa gtaattttca aaataaaaaa
    12781 atattaatat tggggttagc caaatcgggc gaagcagcag caaaattatt gaccaaactt
    12841 ggtgctttag tgactgttaa tgatagtaaa ccatttgacc aaaatccagc ggcacaagcc
    12901 ttgttggaag aggggattaa ggtcatttgt ggtagccacc cagtagaatt attagatgag
    12961 gactttgagt acatggttaa aaaccctggg attccttatg ataatcctat ggttaaacgc
    13021 gcccttgcaa aggaaattcc catcttgact gaagtagaat tggcttattt cgtatctgaa
    13081 gcgcctatta tcgggattac aggatcaaac gggaagacaa ccacaacgac aatgattgcc
    13141 gatgttttga atgctggcgg gcaatctgca ctcttatctg gaaacattgg ttatcctgct
    13201 tcaaaagttg ttcaaaaagc aattgctggt gatactttgg tgatggaatt gtcctctttt
    13261 caattagtgg gagtgaatgc ttttcgccct catattgctg tcatcactaa tttaatgccg
    13321 actcacctgg actatcatgg cagttttgag gattatgttg ctgctaaatg gatgattcaa
    13381 gctcagatga cagaatcaga ctaccttatt ttaaatgcta atcaagagat ttcagcaact
    13441 ctagctaaga ccaccaaagc aacagtgatt cctttttcaa ctcaaaaagt ggttgatgga
    13501 gcttatctga aggatggaat actctatttt aaagaacaag cgattatagc tgccactgac
    13561 ttaggtgtcc caggtagcca caacattgaa aatgccctag caactattgc agttgccaag
    13621 ttatctggta ttgctgatga tattattgcc cagtgccttt cacattttgg aggcgttaaa
    13681 catcgtttgc aacgggttgg tcaaatcaaa gatattacct tctacaatga cagtaagtca
    13741 accaatattt tagccactca aaaggcttta tcaggttttg ataacagtcg cttgattttg
    13801 attgctggcg gtctagatcg tggcaatgaa tttgacgatt tggtgccaga ccttttagga
    13861 cttaagcaga tgattatttt gggagaatcc gcagagcgta tgaagcgagc tgctaacaaa
    13921 gcagaggtct cttatcttga agctagaaat gtggcagaag caacagagct tgcttttaag
    13981 ctggcccaaa caggcgatac tatcttgctt agcccagcca atgctagctg ggatatgtat
    14041 cctaattttg aggttcgcgg ggatgaattt ttggcaacct ttgattgttt aagaggagat
    14101 gcctaatgcc taagaagatt ttatttacag gtggtggaac tgtaggtcat gtcaccttga
    14161 acctcattct cataccaaaa tttatcaagg acggttggga agtacattat attggtgata
    14221 aaaatggcat tgaacataca gaaattgaaa agtcaggcct tgacgtgacc tttcatgcta
    14281 tcgcgacagg caagcttaga cgctattttt catggcaaaa tctagctgat gtttttaagg
    14341 ttgcacttgg cctcctacag tctctcttta ttgttgccaa gcttcgccct caagcccttt
    14401 tttccaaagg tggttttgtc tcagtaccgc cagttgtggc tgctaaattg cttggtaaac
    14461 cagtctttat tcatgaatca gatcggtcaa tgggactagc aaacaagatt gcctacaaat
    14521 ttgcaactac catgtatacc acttttgagc aggaagacca gttgtctaaa gttaaacacc
    14581 ttggagcggt gacaaaggtt ttcaaagatg ccaaccaaat gcctgaatca actcagttag
    14641 aggcggtgaa agagtatttt agtagagacc taaaaaccct cttgtttatt ggtggttcgg
    14701 caggggcgca tgtgtttaat cagtttatta gtgatcatcc agaattgaag caacgttata
    14761 atatcatcaa tattacagga gaccctcacc ttaatgaatt gagttctcat ctgtatcgag
    14821 tagattatgt taccgatctc taccaacctt tgatggcgat ggctgacctt gtagtgacaa
    14881 gagggggctc taatacactt tttgagctac tggcaatggc taagctacac ctcatcgttc
    14941 ctcttggtaa agaagctagc cgtggcgatc agttagaaaa tgccacttat tttgagaaga
    15001 ggggctacgc taaacaatta caggaacctg atttaacttt gcataatttt gatcaggcaa
    15061 tggctgattt gtttgaacat caggctgatt atgaggctac tatgttggca actaaggaga
    15121 ttcagtcacc ggacttcttt tatgaccttt tgagagctga tattagctcc gcgattaagg
    15181 agaagtaaat ggcaaaagat aaagagaaac aaagtgatga caagctcgtt ttgacagagt
    15241 ggcaaaagcg taacattgaa tttttaaaga aaaagaagca gcaagctgag gaagaaaaaa
    15301 aactcaaaga gaaattattg agtgataaaa aagcgcagca gcaagctcaa aatgcttctg
    15361 aagcagttga gcttaaaact gatgagaaaa ctgatagtca ggaaattgag tcagaaacga
    15421 cgtcaaaacc taaaaaaccc aaaaaagtta gacaacccaa ggaaaaaagc gcgacacaaa
    15481 tcgcttttca aaaatccttg cctgttcttt tgggggcgct cttacttatg gcggtgtcta
    15541 tttttatgat cactccttat agcaaaaaga aagagttttc tgtaagagga aaccatcaaa
    15601 cgaaccttga cgaattaatc aaagctagca aagtcaaagc atctgactat tggttaacgt
    15661 tgttaacttc gcctggtcag tatgaacgac cgattcttcg tactattcca tgggtgaaat
    15721 ctttacatct ctcttaccaa tttcctaatc actttctatt taacgttatt gaatttgaaa
    15781 tcatcgctta tgcacaagtc gaaaacggtt ttcagcctat tttggagaat ggaaaacgtg
    15841 tggacaaggt cagggcatca gaactaccga aatctttctt gattcttaat ttaaaagatg
    15901 agaaagcgat ccaacagtta gttaagcaat taacgacatt acctaaaaaa ttagtcaaga
    15961 atatcaagtc agtgtctctt gcaaattcca aaacgacagc ggatttacta cttattgaaa
    16021 tgtatgacgg taatgtagtt agagtaccgc agtcacaact cacattgaaa cttccctatt
    16081 atcaaaaatt gaaaaaaaac cttgaaaatg atagtatagt ggatatggaa gttggaattt
    16141 acactacaac acaggagatt gaaaatcaac ctgaagttcc tcttacgcca gaacaaaacg
    16201 cagctgataa agaaggagat aagcctggtg agcatcagga acagacagac aatgattcag
    16261 aaacgccagc aaatcagagt agtcctcagc aagcaccacc atccccagaa acggtcctcg
    16321 aacaggccca tggctagcta atatctaagt tgaaaaagca atgaaaacgt tagaaattca
    16381 acgattctaa cccataatga attgcctaaa aaaaattaag tttatataac aaaaaacgta
    16441 aaatgataac attttacgtt tttttatggt ataatatttt ctgaatgatt ctgtttttta
    16501 gcagttttta gaatagcaaa agtttggaaa gtaagtgagg tcaagtgaat ggctagaaat
    16561 ggctttttta ctggtttgga cattggaaca agctcgataa aagttttagt agcagaattt
    16621 atttctggtg agatgaacgt cattggtgtt agtaatgttc caagtaccgg cgtaaaagat
    16681 ggcataataa tcgatataga ggcagctgcg actgccatca aaactgcggt agaacaagca
    16741 gaagaaaaag cagggatgac aattgaaaag gttaatgttg ggctaccggc aaaccttctt
    16801 caaattgaac caacacaagg aatgattcct gtcccaagtg agtctaaaga gataaaagat
    16861 gaggatgttg atagcgttgt taaatcggct ttaacaaaaa gtatcacacc agaacgagag
    16921 gttatctctt tagttccaga agagttcatt gtggatggct ttcagggcat tcgagatcca
    16981 cgtggtatga tggggattag attagagatg cgcgggctta tttatactgg accaagcacc
    17041 attttacata atctgcgtaa aacggtagaa agagcaggca ttaaagttga aaacatcatt
    17101 atttctccgt tagctatggc taaaaccatt ttaaacgaag gtgagcgcga gtttggagct
    17161 actgtaattg atatgggagg tggacagaca actgtcgctt ctatgcgagc acaagaattg
    17221 cagtatacca atatatatgc tgaaggcggc gaatacatta ctaaagatat atcaaaagta
    17281 ttaaaaacgt ctttggctat tgcagaagca cttaagttta attttggtca agcggagata
    17341 tcagaagcta gtataactga aacagtaaaa gttgatgtgg taggtagtga agagcctgtt
    17401 gaggtaactg aacgttattt atctgaaatt atttcagcgc gtattcgtca tattttagat
    17461 cgtgtgaagc aagatttgga aagaggtcgt ttactagact taccaggagg cattgttttg
    17521 attggtggcg gtgcaatcat gcctggagtg gtagaaattg cacaagaaat ctttggagta
    17581 actgtaaagc tccatgttcc aaatcaagtc ggtattagaa atccaatgtt ttcaaacgtt
    17641 atcagtttgg tagaatatgt tggtatgatg tctgaagtag acgttttagc acaaactgca
    17701 gtttcaggag aagaactttt gcgacgcaag cctatcgatt tcagtggtca agaatcttat
    17761 ttaccagatt atgatgattc aagaagacca gaatcgacca ttggctatga acaacaagcg
    17821 tcacaaacag catatgattc acaagttccg agtgatccta aacaaaaaat ttcagaacgt
    17881 gttcgtggca tatttgggag tatgtttgat taaaagtaat aaagtgagga gataaaatgg
    17941 cgttttcatt tgatactgca tcaattcaag gtgcaattat aaaagtaatt ggagtcggcg
    18001 gaggtggcgg aaatgccatt aatcgtatga ttgatgaagg tgttgctggt gtcgagttca
    18061 tcgcagcaaa tacagacatt caggcattaa gctcatcaaa agctgaaacg gttattcaac
    18121 taggccctaa attaactcgt ggacttggtg ctggaggaca acctgaagta ggacgtaaag
    18181 ctgctgaaga aagcgaagaa attttaacag aagctcttac aggagcggat atggtattta
    18241 ttactgccgg tatgggtggt ggctctggga caggggctgc accggttatt gctcgtatcg
    18301 ctaagagttt gggagccttg acagtagctg ttgttactcg cccgtttggt tttgaaggta
    18361 acaaacgtgg taattttgct attgaaggta tcgaagaact ccgtgaacaa gttgatactt
    18421 tgttaattat ttcaaataat aaccttcttg agattgttga taaaaagaca cctttattag
    18481 aagcacttag tgaagctgat aatgttttac gtcagggagt tcaagggata accgacttaa
    18541 ttactagtcc tggccttatc aatctcgatt ttgccgacgt gaaaacagtt atggcaaata
    18601 aagggaatgc cttaatgggg attgggattg gttctggaga agagcgcatt gttgaggcgg
    18661 cgcgtaaggc aatctattca cccctattag aaacgactat tgatggtgca caagacgtta
    18721 ttgtgaacgt tacaggaggt ctcgacatga cactcacaga agctgaagaa gcctctgaaa
    18781 ttgttgggca agctgctggt caaggcgtta acatttggtt aggaacatct attgatgata
    18841 ctatgaaaga tgacatccgt gtgactgttg tagcaactgg agtgcgccaa gaaaaagccg
    18901 aacaagtttc aggttttcgt cagcctagga cttttaccca aaccaacgcg cagcaagtag
    18961 cgggtgcaca atatgcatca gatcaagcaa aacagtcggt tcaaccaggg tttgatcgtc
    19021 gctcaaattt tgattttgac atgggggagt ctcgcgagat accaagtgca caaaaggtaa
    19081 tttctaatca taatcaaaat caaggttctg cttttggaaa ttgggatttg agacgtgata
    19141 atatttctcg tccaacagaa ggtgaattgg ataaccatct taatatgtca acgttctcag
    19201 ctaacgatga cagtgatgat gaattagaaa cgcctccatt ctttaaaaac cgttaataat
    19261 ggatttactg acaaataaaa agaaaatttt tgagactatc cgcttatcta cagaggcagc
    19321 aaataggact aatgatagtg tttcagttat tgctgtaaca aaatatgtgg atagtacaat
    19381 tgcaggtcag cttatcgaag caggaattga gcacattgcc gaaaaccgtg ttgataaatt
    19441 tcttgaaaag tatgatgcgt taaagtatat gccagtaaag tggcatttaa tcggtacctt
    19501 acaacgtcgt aaagtcaagg aagttatcaa ttatgttgat tattttcacg ctctagattc
    19561 tgtgagatta gctttggaaa tcaacaagag agctgaccat cctgtgaagt gttttctaca
    19621 agttaatatt tctaaagaag agagtaaaca tggttttaac atttctgaga ttgatgaagc
    19681 gattgaagaa ataggtaaga tggagaagat acagttagtt ggtttaatga ctatggcacc
    19741 agcaaatgcc agtaaagaaa gtattataac tatttttcga caagcaaatc aattaagaaa
    19801 aaacttgcag ttaaaaaaaa gaaagaatat gccttttaca gaattgagca tgggcatgag
    19861 taacgattat ccaattgcta ttcaagaagg ctcaactttt attcggattg gtagagcttt
    19921 ctttcactaa tggagaataa gatggctttt aaagatacat ttaacaagat gatttcttat
    19981 tttgacacgg atgaggttaa cgaagttgaa gaagatgttg cagcatcaac tgataacgtt
    20041 attccaagat cacaacaatc agtcagagca agtagtcatc caaaacaaga acctagaaac
    20101 aatcacgtac aacaagatca tcaggcgaga tcccaagaac agacaaggtc acaaatgcat
    20161 ccaaaacatg gtacttctga acgctattat cagcagtctc agccaaaaga aggccatgaa
    20221 atggttgaca gaagaaaacg gatgagcact tctggtattg caaatcgccg tgagcagtat
    20281 caacaatcaa cttgttcaga tcagacaact attgccttaa aatatcctcg taaatatgag
    20341 gatgctcaag aaattgtgga tcttttaata gttaatgaat gcgttttgat tgattttcag
    20401 tttatgctag atgctcaggc tagacggtgt ttagatttta ttgatggtgc tagtaaagtg
    20461 ctctatggta gcttacaaaa ggtcggctct tcaatgtact tactggctcc gtcaaatgta
    20521 tccgtcaata tagaagaaat gactatccca catactacac aagatattgg ctttgatttt
    20581 gatatgaaaa ggcggtaaat aaatgatatt aatactatct attcttcttc gtctgatcaa
    20641 agtttacact tatttattga ttgcttacgc attaatgtca tggtttcctg gggcatatga
    20701 ttcaaaaatt gggcgtttga ttagtggtat cgttgaacca attttaaaac cttttagagc
    20761 atttaattta caatttgccg gtcttgactt cactattttt gtcgtcatta ttagtttgaa
    20821 ttttttagct caagttttgg tccgtgtgtt tatttaatgg ttagtcatag taagatttat
    20881 cagcattttc accaagaaga atatcctttt attgatagaa tgtctgatat gattaataga
    20941 gttggagatt actatctttt agaagttact gagtttttaa atcctagaga agtgatgatt
    21001 ttaaaaagtt tgattgcttt aacagatcta aaaatgttcg tatcaacaga ttactaccca
    21061 agcgaatatg gtcgtgtcat tattgcacct ggttactatg acttagaaca aagtgatttt
    21121 caaatagctt tagtagagat aagttatcag gcaaagttta atcagttgac acatagtcaa
    21181 attttaggaa ctttaattaa tgaattagga gtaaagcgaa atttatttgg agatgttttt
    21241 gttgaaatgg gatatgccca gctcatgatt aagcgggagt tattggatta ttttttagga
    21301 acaattacta aaatagctaa aactagtgtg aaattaagag aagttaactt tgatcagtta
    21361 attaggtcta ttgataacag ccagaccctg gatattctag tttctagttt tcgattagat
    21421 ggtgtagttg ctactatctt aaaaaaatct cgaacgcaag ttatagcatt aattgaagca
    21481 aataagatta aggtaaacta tcgactcgct aataaagctt cagataatct agtcataggg
    21541 gatatggtga gtatcagagg tcacgggcgt tttactcttt tagcagataa tggagtgacc
    21601 aaacatggca aacaaaaaat aacactaagt aaaatgatac ataaataagg agatacaatg
    21661 gcacttacaa cgctagaaat taaagataaa acttttaaga caaaattccg aggatactgt
    21721 gaagaagaag tcaatgaatt ccttgatatt gttgttgatg attacgaagc tcttgtacgt
    21781 aaaaatcgtg ataacgaagc aagaattaaa gatcttgaag aaaaattatc ttactttgat
    21841 gaaatgaaag agtcacttag ccagtctgta atcttagctc aagaaacagc tgaaaaagtg
    21901 aaagcaacgg ctaatgcgga agcaacaaac ttggttagta aagcgactta tgatgctcag
    21961 catttattag atgaatctaa agctaaagcc aatcaaatgt tgcgtgatgc aactgatgag
    22021 gctaagcgag ttgcgattga aacagaagaa ctaaagcgtc aaacacgtgt gttccaccaa
    22081 cgtttaatct catctattga gtcacaatta agcttatcaa attcacctga atgggatgaa
    22141 ctgctacaac cgactgcaat ctatcttcaa aattctgacg atgcttttaa ggaagtcgtg
    22201 aagaccgttc ttaatgaaga cattcctgaa tctgatgata gtgcctcttt tgatgcaaca
    22261 cgtcagttta ctccagaaga gttagaagaa ttgcaacgtc gtgttgatga aagcaataag
    22321 gagttagagg cttaccaact tgactctcaa tctgattcta cgactgagcc agaggtaaat
    22381 ctcagtgaaa cacaaacgtt taaattaaat atctaaataa agaaaaacaa gatgatttgc
    22441 ttataatcac agcaagcggg agatgatgag agtctcgtat tccctaagta ttatcattat
    22501 cattttctaa aattttcgct gaactacagt aagcgaggag gtttgttcac cttacgagca
    22561 aagagggagc ggtaagtctt atgatatatt atcgctctga actaaggtgg taccacgagc
    22621 tttcgtcctt tttgagacga ggcttttttc gttatagtcg caagtttgtt taaaaggagt
    22681 ttgaaataat gaaattaaaa gagacactta atctaggaaa aacagccttt ccaatgcgcg
    22741 caggtcttcc taataaagag ccacaatggc aagcagcttg ggaacaagca gagctttaca
    22801 aaaaacgtca agaattgaat gcaggtaagc ctgcctttca tcttcatgat ggacctccat
    22861 acgctaacgg caatattcac gttgggcatg cccttaataa aatttcaaaa gatatcattg
    22921 tgcgttctaa gtccatgtct ggctttcaag cgccttatgt acctggttgg gacacacacg
    22981 gccttccaat cgaacaagtc ttggctaagc aaggaatcaa acgtaaggag atggatttag
    23041 cagaatacct tgagatgtgt cgtcaatacg ctttaagtca ggttgataaa cagcgagatg
    23101 atttcaaacg gttaggtgtt tcggcagact gggaaaatcc ttatgtcact ttggacccac
    23161 agtttgaagc tgatcaaatt cgtgtttttg gagcgatggc tgaaaaaggt tacatctatc
    23221 gtggtgctaa acctgtttat tggtcatggt cttcagaatc ggccttggca gaagctgaaa
    23281 ttgaatacca tgacatcgat tcaacctctt tatactacgc caataaagtt aaagatggta
    23341 aaggtatcct tgatactaat acgtatatcg ttgtttggac aacaacacca tttacggtta
    23401 cagcatcacg cggtttgact gttggtcctg atatggatta tcttgtggtg aaacctgctg
    23461 gttcagaccg tcaatatgtt gtggcagaag gtcttttgga tagccttgct ggaaaatttg
    23521 gctgggaatc ttttgaaact ttagctagcc ataaaggagc tgatttagag tacattgtga
    23581 cagaacaccc atgggatact gacgtagaag aattggttat ccttggtgac catgttaccc
    23641 ttgagtcagg gacagggatt gtccacacag caccaggttt tggtgaggat gactacaatg
    23701 ttgggacaaa atataaactg gaagttgctg tgactgttga tgaacgtggc ctaatgacgg
    23761 aaaatgcagg tccagatttc cacggacaat tttataacaa ggtaacgccg attgtcattg
    23821 ataaacttgg tgatctttta ttggcacaag aagtgatcaa tcactcttac ccatttgact
    23881 ggcggacgaa aaaaccaatc atctggcgtg ctgtaccgca atggtttgct tctgtttctg
    23941 atttccgtca agatattttg gatgaaattg aaaaaacaac ctttcatcca tcatggggtg
    24001 aaacacgcct ttacaatatg attcgtgatc gtggcgactg ggttatttct cgtcaacgtg
    24061 cttggggagt tccgcttcct atcttctatg ccgaagatgg cacagctatt atgactaaag
    24121 aagtgacaga tcatgtggct gatttgttcc aagaaaatgg atcaatcatt tggtggcaaa
    24181 aagaagctaa agacctatta ccagagggct tcactcatcc agggtcacca aatggcgagt
    24241 ttacaaaaga aactgatatc atggacgttt ggtttgactc tggttcttct tggaatggag
    24301 tcatgaatac taaagaaaat ctttcctatc cagcggatct ctacttagaa ggctctgacc
    24361 agtatcgtgg ttggttcaat tcatccttaa ttacatctgt ggctgttaat gggcatgctc
    24421 catataaggc tattttatcg caaggctttg ttcttgatgg taagggcgaa aaaatgtcta
    24481 aatcaaaagg caatatcatt tctcctaatg atgtggctaa acaatatggt gcagatattc
    24541 ttcgactttg ggtagcttct gtggataccg ataatgatgt ccgtgtttct atggagatcc
    24601 ttggtcaagt ctctgaaact taccgtaaaa tccgtaacac ccttcgcttc ttgattgcta
    24661 atacatctga tttcaaccct gctacggata cagtagctta tgcagattta ggagctgttg
    24721 ataagtacat gacaattgtc tttaatcagt tggtagcaac gattactgac gcttatgagc
    24781 gttatgactt tatggctatt tacaaagcag tggtgaactt tgttacggtt gatttatcag
    24841 ccttctatct tgattttgct aaagacgttg tctatattga agctgccaat agcttagaac
    24901 gtcgtcgcat gcagacagtc ttctatgata tcttggttaa gattaccaag ttattgacgc
    24961 caatcttgcc tcacactact gaagaaatct ggtcttatct agagcatgag tcagaagcat
    25021 ttgttcaatt ggcagaaatg cctgtggcag aaactttctc agctcaagag gatattttag
    25081 aagcttggtc tgctttcatg actttgcgta cccaagctca aaaagctttg gaagaagcgc
    25141 gaaatgctaa gattatcggt aaatcattgg aagcccattt gaccatctac gctagcgaag
    25201 aagtgaaaac cttattgact gctttagaca gcgatattgc tttgcttttg atcgtgtctc
    25261 aattaaccat tgctgacttg gcagatgcgc ctgcggatgc agtggcattt gaaggtattg
    25321 cctttatggt agaacatgcc ataggtgaag tttgtgagcg ttcacgccgc atcgacccaa
    25381 ctactcgcat gcgttcttac aatgcatttg tctgtgatca cagcgctaaa atcattgaag
    25441 aaaatttccc agaagctgtg gctgaagggt ttgaagagag tgacaaataa gtcttatcaa
    25501 gacttgaagt gacactgagt aagaagacaa ccatcgggtc ttagggcctg atggtttttt
    25561 agtagagctg attacgtcca ttagccagct ttaacgagag acaaagtagg agaaatttag
    25621 agtacttttt taatttcttt taagaaaatt gctggcttca tataaggact attaagatag
    25681 agttcacctt tcgctaataa aaaaacttag ctcttttaga agggctaagt tttgtctgaa
    25741 tagctggctg ctgtttatac agaagtttga aatgaaattt caataaggtt attcgtgtga
    25801 agtgtttttg attgtccagg aataacaatt ttaggatcaa ttttacgttt taaaaaaaat
    25861 tcgcgaatga tatcaagttc atcctccttg atggcacggt atttatttga aataagtagt
    25921 tcgtaatgtt ttggtgcttg ggtaaaaatg acatctgtat ttcctgctga gtatacttcg
    25981 actaaatagg catcggtgtt ttttagctga tttctgatga gttctgggtg actgctagtg
    26041 gtattgatga gtctcataaa tgtcctcctt agtattcctg tacctattat agcactaatt
    26101 taaagcctta gttagtagaa tgatgctttt tttgccattc cttgactccc cattgatggc
    26161 tctctcgttt taatttagca attgcctcga taggagaaaa ccaacttaag ttattaaaat
    26221 cttctaaggg cttagaaacg gcttgaaatg cggttacttc ataaagatag gcaggatggt
    26281 agaagtgggt atcacgatgg cgagagtaga aatattcatc agcctgaccg taatatgaac
    26341 caatagtagc agaaaaacca agttcttcaa tcagttcacg ctctagggcc tgaagctgac
    26401 cttcacctgc ttcaatttca ccaccaggta aaaaccagga accattagga gcttgaacaa
    26461 gaataatttt tgtttgttcg tgattgggaa tgatagcata gacaccgtaa cgtgtgacat
    26521 agtctttatg tgcattttta tgtccaaatg ttggaatcat cattttattt acctttcctt
    26581 tttcttcatt atactaaatt tttgctaatt attcttatca atttgtttag tttaataaaa
    26641 aattgataga aaaagagaga gtctccttat agaaactctc ttagctaacg ttaatctaga
    26701 gactcttcgt taagaaaaga atctccctct ttaacagctt taatcgtgat gttttcactg
    26761 tttgtcatca gtgcacgtaa ctgtttttca gttgggtgtt ctaaataata gtctgtaatg
    26821 gcatcttcga tataatcttg gatagtacga cggagcggcc gagcgcccat tttaggatca
    26881 tagcctaaat ctactaattt ttctttgacc ttttgtgtca catcaagatg aatcccatta
    26941 tagcctaagc gctcattaac atcttccagc attaaatcaa cgatatgcag caaatgctct
    27001 ttagataaag ctttgaattc aataatacca tcgaaacgat tcataaactc tgggctgaag
    27061 aagttgctta attcaccaag gacagaactt gtccgtcctt ctctagcagc accaaatccg
    27121 acagaagctt cgcttttacc tgttccggca ttagaggtca tgataataat ggtgtccttg
    27181 aagctgactg ttcgcccttg gccgtctgtt aagcgaccgt catcaagaac ttgtaagaac
    27241 atgtgcatga cgtcaggatg ggctttttcc acctcatcta agagaataag ggaataagga
    27301 ttgcgacgaa cttgttcggt tagctgtcca gcttcttcat agccgacata acctggagga
    27361 gccccgacta atttggcgac agcgtgtttt tccatgtatt cggacatgtc aaagcgaatc
    27421 atattgtttg tcgaaccaaa gagttcaatg gctagttgtt tagacaattc agttttacca
    27481 accccagtcg gtccaacgaa taagaaagaa ccaatagggc gatttggagt tcctaatccc
    27541 acacggttac gacgaatagc cttagcgatt ttatcaacag catcgtcttg cccgatcacg
    27601 tgtgctttca gatcattggc taagtttacg agttgagact gttccttttc ttttaagtcc
    27661 ccaactggaa tattagtttt ttgctctaca atagcttcaa tggttttttc agtaatgatg
    27721 ggaatatctt gctcatcgac tttttgagcc tgcatttctt tgtattttgt aatttgatcg
    27781 cggaaataag ctgcgcgttc atagtcttcg tctcgagtag cttgcgcctt gagattctca
    27841 gcttcaataa gacgtttgtc aatttcttta ggatcaacaa agttgagggt tagattcatt
    27901 ttggaaccag cttcgtccag aaggtcaata gccttatcag gaagaaaacg gtcttgaata
    27961 tagcggttag ataaatgagc ggcagcttca atagcggctg ggctatattt gacatgatga
    28021 tagtcttcgt atttcggttg gatacctttt aaaatcgtga tggtttcctc aacagaaggt
    28081 tcatcaactt taacgggttg catacgtcgt tctaaggcag cgtctttttc aataatacgg
    28141 tattcattta atgtagtagc accaacgagt tgcaactcac cacgggccaa ggctggtttt
    28201 aaaatattac cagcatccat attgccgtct cctgcagaac cagcaccgac aatctcatga
    28261 atttcatcaa taaagagaat cacatccttg cgattgcgaa tttcttccat taatttttgc
    28321 atgcgctctt caaactgacc acggatacct gttccctgaa cgaggctaac cacatcaaga
    28381 cgaatcactt gcttgccttg gagtttttga ggaacagtac catcaataat tttttgagcc
    28441 aaaccttcta caacagcagt tttaccaact ccaggctcac caattagcac aggattattt
    28501 ttagtacggc ggttgaggat ctcgataacg cgtgtaatct cttcgtcacg accaataaca
    28561 ggatcaatat taccatttct tgcaatatct gtgacattaa tcccaaactc ttctaataag
    28621 ccctttgctt gctgatttgg tgtttgtggc tgagcaggtc gttgtccgtt gtagttacca
    28681 ccatagcgtc ctccgccatt tccattttgc cctgcctgag taggtggggt atttggaaga
    28741 ttaccaaaag ctctaaaatt attcaaatca ccaaaaaagt catcaaagaa aggacttgtg
    28801 gatctatctt gtgctctata tcctggggtt aggccattta aaatagaatt ggcaggatcg
    28861 gatttcatga tttgataaca attttgacag agatcaacct gtctttgttt tccattaaca
    28921 cttgtataaa gatgaatagt agattcgttt aaattacaat tttgacacag cataatatac
    28981 ctccgagggt ttatctaaaa gtcaaagtca tttagtcaaa aatggtcaaa ctttatattc
    29041 atattatagc accacggctt gggaaatcaa gtaaaaagtt tatcgcttta gcgttagctt
    29101 ttagagagta acatgacaga gtaactttat taagtttcca atgagcaaat gtttaaaatc
    29161 attgaaaaga gtaaaaaaag taaaatatca ataaatatat tactttcctt gttagtttcc
    29221 cttgttttgt gttaaaataa gagggataaa aaacgaatag gagaaacgaa atggaatcac
    29281 atttggtgag aatcatcaat cgtttggagt tgatggtgac agatggcggt aatctcaagc
    29341 gaaattttga acgcgatggt gttgttgttg cagaagttgc cttttcaaat gatcctgaaa
    29401 atggacctgt tttcacattg cgtgatgttg aagcgcgtga gtcatattcc tttgatagca
    29461 ttgacttgat tgcaatggaa atctacgatt tattatatta agcttgacat agtaaagaga
    29521 cttgcctgag gcaggttttt ttattgtcaa aaagtattgt attaatattc atcagatggt
    29581 ataattatct ttattgtaat gaacctagga cttatgagga gatataaatg gatttgtcat
    29641 ttttgcccaa atactgggcc tactttaact acggtgtact tgtcaccatt atgatttcag
    29701 tcagcgttgt cttttttgga acccttattg gtgtcttggt aaccctgatt aagcgtagtc
    29761 atgtgaagcc gttgacctgg gtcgttaatc tttacgtttg gatctttcgg ggaacaccta
    29821 tggtggttca aatcatgatt gcctttgctt ggatgcattt taacaatatg cctactattg
    29881 gttttggggt tttagacttg gacttttcaa gactacttcc tggaattatt atcatttcat
    29941 tgaatagcgg tgcttatatt tcagaaattg ttagagcagg tattgaggct gtaccaaaag
    30001 ggcaattaga agcagcttat tcactaggta ttcgtcctca aaatgccatg cgttatgtga
    30061 ttttgcctca ggcctttaaa aatattttgc cagccttagg aaatgaattt attaccatta
    30121 ttaaggatag tgctttttta caaaccattg gagtgatgga actttggaat ggtgcccaat
    30181 cagtggtaac ggctacttat tctccaattt cccctttact ggtggctgct ttttactact
    30241 taatggtcac aacagtgatg gcacagttat tggcagtctt agaacgtcac atggcgcaag
    30301 gaggtaatca ttgatgactg aggccattat ttcaattaaa gacttgcaca agtattatgg
    30361 gcacaatgaa gtgcttaagg gaattgattt agacattatg ccaggagagg ttgtggtgat
    30421 tattggcccg tctggttctg gaaaatcgac cttacttcga accatgaatc ttttggaagt
    30481 accaactaag ggacagatta ggtttgaggg gattgatatt accgataaga agaacgatat
    30541 tttcagcatg cgtgaaaaaa tggggatggt ttttcagcag tttaacctct ttcccaatat
    30601 gactgtttta gaaaatatca ctttatcgcc aatcaaaact aagggaatgg ctaaagcaga
    30661 ggctgacaaa acagccttga gcttgttgga caaagttgga ttatcagaaa aagccaaggc
    30721 ttatcctgct agtctttctg gtgggcaaca gcagcggatt gcgattgcgc gtggactggc
    30781 tatggatcca gatgttttac tctttgatga accaacttca gctctagacc cagaaatggt
    30841 gggtgaggtc ttggctgtca tgcaagattt ggctaaatct gggatgacta tggttattgt
    30901 gactcatgaa atgggctttg ctaaagaagt cgcagatcgt gtgattttta tggatggtgg
    30961 cgttattgtg gaagaaggaa gtcctaacca actttttgat ctgactaaag aagaacgtac
    31021 aaaagatttt ttgagtagag tattataaat ctgttctatg aattataaaa ggaagtccta
    31081 aatcatttag gacttccttt tataatttgc taaggcattt tataggagat tacttttctt
    31141 tttgttaaaa taaaaagaaa tagttacatt atttgcatag aagaaaggga tctgttatgg
    31201 tttcagtgat gtggtttcgt agagatttaa ggattgatga tcaaaaagct ttagctcatg
    31261 ctattgctag tcaatcgcca accttatgtg tgtttcaatt taacaaggag caattaaacc
    31321 tgctacatag tcggaatcaa tctagtttta ttactagcgt tttggctttt agaaagttat
    31381 taaaatgtga aagtattgac ttatatctaa tgtatggaga cttaatgacc tgttttgagc
    31441 aattattgac acaattaaaa gactggacag atgtgtattt taactatgat gaatctggat
    31501 atggtcgttt acgtgatcaa aaagcagcac aatttttcaa aaaaaatggg attgctgttc
    31561 atacctacca agatcactat ttacatggta gtcaagagat cattaaccaa tcaggtcagc
    31621 cttataaagt ttttacgccc tactatagga tttggcaaaa ttatccgaaa gagactccta
    31681 ttaaagtaga actatcgcaa ggcaggtggt taaatcttga gacaccagat gatgttttaa
    31741 ggactgttga gagctttaaa gatgaaaaat atcaggatgt tgcaactttt gatgaagcgt
    31801 caaaacaact aaatcgattt attcaagacc aattagcagc ttatcacgct aatcgagatt
    31861 ttccagcgca gctagggacg agtcggttgt ctcccttttt aagaatagga gctattggca
    31921 ttcgcacggt atatcacgct gtgcgtcaag caccaaatag tctagggcaa gctacttttt
    31981 taaaagaatt agcttggaga gacttttaca atatggtcta tgtggcttat cctgatcaga
    32041 aaactcagcc tatccaaaaa gccttttctc aaatcgaatg ggttaataat cctgactggt
    32101 ttcagttgtg gaaagaaggt aaaacgggtt accctattgt tgatgctgcc atgcttcagt
    32161 tacaaaagac aggatggatg cataatcgtt tacgaatgat agtagcttct tttttaacta
    32221 aagatctttt atgtgattgg cgtttaggag agcagtattt tcaacaacaa ctaattgatt
    32281 atgatgctgc cagtaatatc ggaggatggc agtgggctgc ttccactgga acagatgctg
    32341 ttccttattt tagaatattt aatcctgtta ctcaaggtaa gcgttttgat cccaaaggcg
    32401 agtttattaa agcgtatttg cctcaactag aacacgttcc agagaagtat ctccacgaac
    32461 cgtggaagat gccaaaatac cttcaagaga gtgtatcctg tattattggc acagactatc
    32521 ctcaaccaat tgtcgatcat gctaaacaac gtgaacaagc tattgcaaag tatgagtggg
    32581 caaaagaaaa agcaaaaata gaatgactgt tctcattata agagtttaaa caaaaagtta
    32641 tcatgaatta agattattta atttaaaaaa taagttgact ttatttagat tatttgatac
    32701 tattcagttg tctcccttag agaaagcgat ttcttgtttt ggagctgatt tttaccattt
    32761 ggtcttagaa taggatgagt aatatgactt acaacgaggt atatcaagaa tggttgcaca
    32821 ataatgatct tagtgatgat attaaagcag atttagcagc cataaaagac aatgaggctg
    32881 agattcaaga tcgtttttac aaaacacttg aatttggaac agcagggcta agaggaaaac
    32941 ttggagcagg caccaatcgc atgaatacct atatggttgg taaggcagca caagctttag
    33001 ctaatactat tattgatcat ggacctgaag cggttaaaaa gggcattgcg gttagttatg
    33061 atgttcgcta tcaatctaga acatttgcag aattaacatg ctctattatg gcagctaacg
    33121 gtattaaggc gtacctttat aaagggattc ggccaacacc aatgtgttcg tacgctattc
    33181 gtgctttggg atgtatctct ggtgttatga ttacagctag tcacaatccc caagcttaca
    33241 atggttataa agcttattgg caagaaggtt ctcaaatttt ggatgacatt gcggatcaaa
    33301 ttgcacaaca catggctgca ctaactcagt atgaggaaat caaacaaatg ccttttgaaa
    33361 aggctctgga cagtggactt gttacttata ttgatgagag tattgaagaa gcatataaaa
    33421 aagaagtgct tggtttaacg attaatgata ctgacattga taagtcagtc cgagtagtct
    33481 ataccccttt aaatggtgtt gggaatctac cagtgcgtga ggttttaaga cgccgcggtt
    33541 ttgaaaatgt ttatgtggta cctgagcagg aaatgcccga tcctgatttt acaacagttg
    33601 gctatcctaa tcccgaagtc cctaaaactt ttgcatattc agaaaaatta ggtaaggcag
    33661 ttgatgctga tattttgatt gcaactgatc cagattgtga ccgtgtggct ttagaagtta
    33721 aaaatgcagt cggtgactat gtttttctca atggcaataa aatcggagcg cttttatctt
    33781 actatatctt ttcacaacga tttgacttag gcaatttacc ggctaatcct gttttagtga
    33841 aatccattgt aacaggagac ttgtcacggg ctattgctag ccattatggt attgaaaccg
    33901 ttgaaacatt gactggtttt aagaatattt gtggcaaggc aaacgaatat gatgtgacga
    33961 aacaaaaaaa ctacctgttt ggttatgaag aaagtattgg tttttgctat ggcacttttg
    34021 ttcgtgacaa agacgctgtt agcgcctcta tgatgattgt agaaatggca gcttactaca
    34081 agaaaaaagg gcagaattta ctagatgttt tgcagactat ttatgcgaca tttgggtatt
    34141 ataatgaacg tcaaattgcc ttggagcttg aggggataga aggacaaaaa cgcattgcga
    34201 ggataatgga agatttcaga cagacaccaa tagctagtgt tgcagagatg gcgttagaca
    34261 agacaattga ttttattgat ggctatcaag attttccaaa gcaaaactgt ctcaaatttt
    34321 atttggatga tggttcttgg tatgcactta gaccatcagg aacagagcct aaaataaaat
    34381 tttatttata tactattggc caaacacaag aaaatagtgc aacaaaatta gatgctattg
    34441 aagcagcgtg tcgcacaaaa ataaatcaag ttaattaaac atcaaaaatg gataacactt
    34501 ttacactatt tttccatata aacaactaca aaaggaggtg agacttttag gcaccttctt
    34561 tttcttgtag gtgccactaa tcaatttaat cgactcaaac caatactaaa aaataagtaa
    34621 gagtataaag aacaggcatc tatttttttg ttataataga aaaatgaaat taaagggaga
    34681 tgtcaatgac agaactaatt gatggtaaag ccttagctca aaagatgcaa caagagttag
    34741 cagctaaagt caacaatcta aaacaaaaaa aaggaattgt accaggctta gccgttattc
    34801 ttgtaggtga tgatcctgct agtcaggtgt atgtccgtaa taaagagcgt gcagctctta
    34861 ctgtaggttt taaaagtgag accgttagat tatcagaatt catttgtcaa gaagagctta
    34921 ttgcagtaat cgaacgttac aatgcagata acactattca tggtatttta gtgcagctac
    34981 ccctgccaaa tcatattaat gataaaaaaa ttattctcgc cattgatccc aaaaaagatg
    35041 tggacggttt tcacccgatg aatacaggtc acctttggtc aggacgtccc ttaatggttc
    35101 cttgtactcc atcagggatt atggaattgc ttcgagaata taatgttaac cttgaaggta
    35161 aacatgccgt cattattggc agatcgaata tcgttggaaa accaatggca cagctcttac
    35221 tagacaaaaa tgcaacagtc acgttgacac attcaagaac acgtcaatta gaagaagtat
    35281 gtcgctgtgc agatgtgttg attgtggcaa tcggacaagg tcatttcata acaaaacaat
    35341 atataaaaga tggtgcgata gtgattgatg tgggaatgaa ccgtgatgat aatggcaagc
    35401 taattggaga tgtggccttt gatgaggtgg cagaagttgc agcgaaaatc acccctgtac
    35461 caggaggtgt cggtcctatg acgattgcta tgttgctaga gcaaacttat caatctgccc
    35521 tccgtagtac tcataaatag ttataattag acaattcaac ataagagaat ataagaagtc
    35581 agcaatggct tccttttttg tttttctgct cactatttat ccagaaaaat ggtataattg
    35641 cattagtatg caaagggact atgtcaggtc caactaagtg aaaggtggct tgatggcaga
    35701 ttatttaacc gtcactcatt tgacgaaata tttaaaatta aaatttgacc gtgaccctta
    35761 tttggaacgg gtttatctga ctggtcaagt gtccaatttt cgaaaacgac caactcatca
    35821 atatttttcc ttaaaagatg aaagtgctgt gattcaagcc accatgtggg caggagtcta
    35881 taaaaaacta ggattcgacc tagaagaagg catgaaaatt aatgtaattg ggcgagtcca
    35941 actttatgaa cctagtggct cttactctat cgtgattgaa aaggcagagc cagatggtat
    36001 aggtgccttg gctttgcagt ttgaacaatt aaagaaaaaa ttaacggcag aaggttattt
    36061 tgagcaaaaa cacaagcagc ccttgccaca gtttgtatca aaaattgggg tcattacaag
    36121 tcctagtggt gctgtgattc gagatattat cacaaccgtt tcacggcgtt ttccaggggt
    36181 tgagatttta ttattcccaa ctaaagtaca aggtgatgga gccgcccaag aagtagtggc
    36241 taatattcga agagccaatc aaagagagga tttggacttg ctcattgtcg gccgtggagg
    36301 tggctcgata gaagaccttt gggccttcaa cgaggaaata gtggttcagg ctatctttga
    36361 atcgcaactt ccagtgattt cgagtgtggg tcatgaaaca gacacgactt tagcggattt
    36421 tgtggcagat cgcagagcag ccacaccaac agcagcagca gagttagcaa cgcctattac
    36481 aaaaacagat cttatgtctt ggatagtaga gcggcaaaat cgttcctatc aggcctgctt
    36541 gaggcggatt aagcaacggc aagagtgggt ggataaactc tcgcaatctg ttatttttag
    36601 gcaaccagaa cgattatatg atgcctacct gcaaaaaatt gatcgcctta gcatgaccct
    36661 gatgaatacg atgaaagatc gtctgagttc cgccaaagaa aacaaggttc agttggacca
    36721 ttccttggcc aatagtcaat tacagacaaa aatcgagcga taccaagacc gtgttgctac
    36781 cgctaagcgt ttactgatgg ctaatatggc tagtcaatat gatagtcagc tggctcgctt
    36841 tgaaaaagca caggatgctt tgctatcact ggatgccagc cgaattatcg cccgtggtta
    36901 tgcaatgatt gaaaaaaatc aagcactggt agcctctgta agtcagataa caaagggtga
    36961 tcagttaacc attaagatgc gtgatggaca attagatgta gaggtaaaag atgtcaaaaa
    37021 cgaaaacatt tgaagaaaat ttacaagatt tagagactat tgtgaacaaa cttgaaaatg
    37081 gggatgtccc tttggaagaa gctatttcag aatttcaaaa aggaatgctt ctctcaaaag
    37141 aacttcaaaa gaccttacaa gcggcagaaa aaacactcgt caaagtgatg caagctgatg
    37201 gcacagaagt agatatggat gattaatgga caaattagct agaattgacg aagccattcg
    37261 tcgctactat aagacgacta gtaacggtgt atctgaagag ttgattgatg ctatcttgta
    37321 ttctgttgac agtggtggca agcgcattcg cccacttatc ttattagaga tgattgaggg
    37381 atttggggta tcgttacaga atgctcattt tgatttggct gcagcccttg aaatgattca
    37441 tacaggaagt ctgattcacg atgatttgcc agccatggat aatgatgatt accgacgtgg
    37501 tagactgacc aatcacaaac aatttggaga ggctactgct attttggcgg gagatagctt
    37561 atttttagac ccctttgggt taatagctca agctgaatta aatagcgagg ttaaagtagc
    37621 cttaatccaa gaactttccc tagcttcagg aacttttggc atggttggtg gtcagatgct
    37681 agatatgaaa ggggaaaatc aagctttgag tctccctcag ttgtcactga ttcacctcaa
    37741 taaaaccgga aaattattaa cttttccttt taaagcagca gctcttgtaa cagaacaggc
    37801 tattactgtt cgtcaacaac tagagcaggc gggaatgctc atcgggcacg cctttcagat
    37861 tagggatgat attttagacg tgacagctag ctttgaagat cttggaaaaa cgcctaaaaa
    37921 ggatttattt gcagaaaaag ctacttaccc aagtttattg gggttagaag cctcctacca
    37981 actgttgaca gagagtttag atcaggcttt gacgattttt cagacactcg aaagcgatgt
    38041 aggctttaag cctcaaataa ttacaaaact gatagaaggg ttacgactta atgcctaaag
    38101 aaagagtaga tgtgttagcc tataagcaag gattatttga gaccagagag caagctaagc
    38161 gtggtgttat ggcaggctta gtggtctctg tgattaatgg ccaacgctat gacaagccag
    38221 gtgacaaaat tgacgatggg actgagttaa aacttaaagg tgaaaaactc aaatacgtca
    38281 gtcgtggtgg attaaaactg gaaaaggggc tgcacgtttt tggtgtatca gttgctaatc
    38341 aaattgggat tgatattggc gcttcaacag gtggctttac cgatgttatg ttacaagatg
    38401 gggccaaact agtctatgcc gtcgacgttg ggactaacca gttggtatgg aaactcagac
    38461 aagatccacg agtaagaagt atggaacagt ataactttcg ttatgctcag ccagaggact
    38521 ttaatgaggg acagcctgta ttggccagta ttgatgtgtc ctttatttct ctcagcttga
    38581 ttttgccagc cctgcataac gttttgtcag atcagggaca ggttattgct ctcattaaac
    38641 cgcaatttga agctgggcgc gagcagattg gtaaaaaagg catcgtcaag gacaaacaga
    38701 ttcatgaaaa agtgattcaa aaggtcatgg attttgcctc aggttacgga tttacagtta
    38761 agggacttga tttttcccct attcagggtg gtcatggcaa tatcgaattt ttagctcatc
    38821 ttgctaagtc acagacacct gaaacgttag ccccgcattt gattcaggaa gttgttgcca
    38881 aggcacataa ggagtttgag aaacatgaaa aagagtgaac gacttgaatt aatcaaaaaa
    38941 atggttttga cgcatccaat tgagacgcaa catgacctct taagattatt ggcagagcat
    39001 gggttagaat taacccaggc tactatttca agagacatga atgaaattgg tatagtaaag
    39061 attccttctg gcagtggacg ttacatctat ggtctttctc aagatagtgg aaaaaagatc
    39121 gttcaaggac ctagatcgat aaagagcact attttagctg tatctgacaa aacaaaaggt
    39181 ttagaacaac acctctattt aaaagtcgta cctggcaata gtaaattaat caaacgttat
    39241 ttattagcag atttttcaaa agctattttt agtcttattg ctgatgatga tagtttattg
    39301 ttaattgcaa agtctccttc agaagcagat atgatacgcc aagaaatttt gctttggatg
    39361 cagggtataa cctagaaaaa aataggggag aaagtgatgt tattagaaat ttctattaaa
    39421 aattttgcta ttattgatga aatttcccta aattttgaaa atggtatgac ggttttgacg
    39481 ggtgaaactg gagcaggaaa gtctattatc attgatgcta tgaatatgat gcttggtgcg
    39541 cgtgctagta cagaagtgat tcgtcgtgga gctaataaag cagaaattga ggggttcttt
    39601 tcggtagatg ctaccccaga actggttgcg tgtctggaat catcaggcat tgctatggaa
    39661 gaagaactga ttattcgccg agatatcttt gccaatggca gaagcgtgag ccgcattaat
    39721 ggtcagatgg ttaacctagc caccttaaaa caggtcggac agtttttggt agatattcat
    39781 gggcaacatg accaagaaga attaatgcga ccacagctcc accagcaaat tttggacgct
    39841 tttggtgata aggcttttga gcaattgaaa gagaactatc agcttatttt tgatcgttat
    39901 aaaagcttgc gtcgccaggt tattgacaaa caaaaaaatg aaaaggaaca caaagatcgt
    39961 attgatatgt tggcctttca gatagcagaa attgaagctg ctgccttgag tcgaggtgag
    40021 gacgaccggt taaatcaaga acgtgatcgc ttaatgaacc acaaacaaat tgctgatacc
    40081 ctgaccaatg cctacgtcat gctagataat gacgattttt caagtctatc caatattcgc
    40141 tctagcatga atgacttact atcaattgag cagtttgatt cagagtacaa agggatgtcg
    40201 acttcgattt ctgaagccta ttatattctt gaagaagtga gcaagcaatt atcagatacc
    40261 attgaccaac tggattttga tggtgggcga ttacaggaaa ttgaatttcg cttggataca
    40321 ctcaatagct taacccgtaa atacggtggt aatgtgaatg atgtgcttga ctactacgat
    40381 aatattatca aagaatacca gctattaaca ggagacgact tgtcttcagg ggatctagaa
    40441 tcagaattga aagccttaga aaaacaattg gttgctgcag ccactaactt gagtgaacgc
    40501 cgccatcaat tggcagagca attagaagct gagattaaag ctgaacttaa ggaactttat
    40561 atggaaaaag cagatttcaa agttcacttt accacatcaa aattcaatcg tgatggtaat
    40621 gaaagcttgg aattttacat ctctactaat ccaggcgaag gatttaagcc ccttgtcaag
    40681 gtggcttctg gaggagagtt atcacgcctt atgttagcca tcaaggctgc tatttctaga
    40741 aaagaagata agacgagtat tgtttttgat gaagtagata caggtgtttc tggtcgcgtt
    40801 gcgcaagcca ttgcccaaaa aatttataaa attgggcgac atgggcaagt tctcgctatt
    40861 tcacacttac ctcaagttat tgctattgct gattatcagt attttatttc taaagaaagt
    40921 aaagaagagt ccaccgtttc taaggtaagg ttattaactc ctgaagaacg tgtggaagaa
    40981 attgctagca tgattgcagg aacagatatg acacaagctg ctcttacgca ggctcgtgaa
    41041 ctacttgcca aacattaaga ttgtgtttgg tatttgatat tttcataatt agatgacatt
    41101 gattttttag gtgctaaaat aatcattgtg ctagaccatt tttcctctgt taatctggct
    41161 tggtttgcct tgacaaacct ctgagaaata gtagaataga taagagaatt ttaagctgag
    41221 gggatagaaa tgactagtaa aaaagcgtgt ttatcaagca tcattgtgtt agcaagttta
    41281 acgtgtggaa atgatactgt tagtgccaat catctctcag caactggaga taagtttgat
    41341 gattgctcaa cacttgttga aaaagatgtg gcccctaaag atgaacttga gatgttagca
    41401 tggtcctcgt ctcaaacaac tgatgatgct gacagagact atgaagattt tctcgatgat
    41461 gattctttta tttctcaaaa tgaaactgat aagatgtttg agaatttaac tgatgatagg
    41521 ttattagatg aattagatga attagatgaa ttagatgaat tagatgaatt agatgaagaa
    41581 gatacaattg agccagagca aaatgtaata atgcctagtg acgatgagct atttgattta
    41641 actgatgctg ttgagacacg ccttactgtt tctagtgctc cccatttaga ggctgaattg
    41701 ccgaaaccac atttgaggag cctatcagat acagcactgc ggtctggtga aattagagga
    41761 catttagata acaaactgga cgctttgtct gtaacagcta caaagttagc attaacgatg
    41821 gctcaaaaat ttgatttgac aacgcatgtc tattctatag gtgaaagctt tagtgaagta
    41881 ttagctgctc attatgaaga cagaaaagca gaatcagctt tttctaagaa aaagagattt
    41941 caccttccta ttgctactcc agatgttgtt atagaggagt taaggcgcct agtctcttct
    42001 attggaagtt caaaagaaga tgtttcagtt ccttataatc ggaagctagg tatggcagtt
    42061 gcaaaaagaa aaatagccct gccacaaacg ggagaggagt tctcttatta tccagcttta
    42121 cttggtttaa tgatattagg attaacgccg attatgatac caaagaagat aaataattag
    42181 gtaataagtt attgtagttg taaaagacgg ggcaaatgtt caagcgtgaa aaggttgact
    42241 tttgcctcgt tttgtatctc gttattgcta aggcattttc ttcgagcagc tagtgctcgc
    42301 catacctatc taccgagtga ttattcaata gcactaccag ttttgcttta tttagtgagg
    42361 agtgttagaa taaagaggtg agaaactagt ttagaatgga aaaatacatg ggaactataa
    42421 aaattgttac agattcatca ataactattg aaccagaatt aattaaagct ttagatatta
    42481 ctgtagtgcc tttatctgtc atgattgata gcaaattata ttctgataat gatttaaaag
    42541 aagaaggtca ttttttgagt cttatgaagg ctagcaaatc ccttcccaaa accagtcagc
    42601 caccggttgg tctctttgct gaaacttacg aaaatttggt caaaaaagga gtaactgaca
    42661 ttgttgctat tcacctctca ccagctttat caggtactat tgaagcttcc cgtcaggggg
    42721 ctgaaatcgc tgaggcccct gttacagttt tagattcagg atttactgat caagccatga
    42781 aatttcaggt cgttgaggca gcaaagatgg caaaagcggg tgctagtttg aatgagattt
    42841 tggctgctgt tcaagctatc aaatcaaaaa cagagctcta cattggtgta tctaccttag
    42901 aaaatttagt aaaaggaggt cgtattggtc gtgtgacggg tgttttgagc tcgctactta
    42961 atgtcaaagt tgttatggca cttaaaaatg atgagttaaa aacgcttgtc aaaggtcgag
    43021 gtaataaaac atttacgaaa tggttagata gttacctcgc taaaaatagt catcgcccga
    43081 ttgcagagat agctatttct tatgctggtg aagctagcct ggcattaacc ttaaaagaaa
    43141 gaatcgcagc ttattacaac cactctattt cggttttaga aacaggctct atcatccaaa
    43201 cacatacagg agaaggagct tttgcggtta tggttcgtta tgaataatcg tcatttattt
    43261 agtgggatat ttttctttgt tattagttta tgtctggcct ttttattgct aaatattatt
    43321 atccctaagt caaattcacg tttgaaaaag agtgattttc tgacaaaaga acaagtagct
    43381 atccaatatg ttgctatagg agattcattg acagaaggag taggtgatct aactcatcaa
    43441 ggtggttttg ttcctttgtt aacgaatgat ctcagtgaat attttaaggc taatgttaat
    43501 catcaaaatt acggcgtatc tggtgatacc agtcaacaaa ttcttgatag gatgataaaa
    43561 caaaagcaga tacagttatc tttaaaaaaa gcagatataa tgacgttaac cgttggtggt
    43621 aatgatgtta tggcagttat tcggaagaat ttagcggatt tgcaagtttc tagttttaga
    43681 aagccagctc gtcagtatca aaaacgatta agacagatta tcgagttagc cagaaaagat
    43741 aataaagatc ttcctatttt tattttaggc atctataatc cgttttattt gaattttcca
    43801 gaactaactg atatgcaaaa agtgattgat gactggaata ccaaaactaa ggaggttgtt
    43861 ggagaatacg atcgtgtgta ctttgtgcca ataaatgacc tcttgtataa agggataaat
    43921 ggacaagaag gaattgttca ttcttcagga gatcaaacta caattgtcaa tgatgccttg
    43981 tttactgggg accattttca cccaaataat actggctatc aaatcatgtc aaatgcagta
    44041 atggagaaaa taaaaaagca tgaaaaaaaa atcaaacctt aattggtgga agtggagctt
    44101 cttatgtctg cttgctttta ataccgcctt tttgatggtt attgcaagtc gtctcattca
    44161 agtaagagaa cctgagtcag aactgattgc taaaaaacca gttaaaaata tcaaaattgg
    44221 tacatttgtc acaaccagag aacagcttaa cgaaactgta gcaagttatc ttaaagatta
    44281 tcagactaaa aaaatgtctt ataaatttta tgcgacatct tcttctattt tgtttgaagg
    44341 aacttatcaa ttattagggt atgaagtccc tttatatatc tattttcaac ctcatcgtct
    44401 agaaaatgga gctgtccaac tacaggtgat ctctttttca gtgggaactc tgccactccc
    44461 agaaaaagat gttttgcagt atttgaaatc aagctacaaa ttgccaagct ttgttaaagt
    44521 gatgccaaat caatcagcta tcgttgttaa tttacaagat atccaaaatg atgctaaggt
    44581 ttatttaaag gccaagaaaa ttgatttatt taatgatgaa atcagtttta acatctataa
    44641 gaaataagct caaataacgt taaatacttg acctaatcgc tttttttaga tatgataata
    44701 tctgttaaag cattaagctt actaattatt tggaggattt gttaaatggc taacaaacaa
    44761 gatttaatcg caaaggttgc agaagcaact gaattgacta aaaaagattc agcagcagca
    44821 gttgatgccg tgttttctac aatcgaagct ttccttgctg aaggtgaaaa agtacaattg
    44881 atcggttttg gtaacttcga agtacgcgaa cgtgcagctc gtaaaggtcg taacccacaa
    44941 actggtgcag aaattgaaat tgcagcttca aaagttccag ccttcaaagc tggtaaagct
    45001 cttaaagacg ctgttaaata attcgtctag aaaaatcttg ttgctatcga tgtttattga
    45061 tagcgacaag gttctttttt atatttgggg catatttggg gcataaagtt agtagttttc
    45121 tcttcagtta agccaagttc taatgcatgc actctataat ggtcagtcat ccttctatta
    45181 gtatatcatt tccgcaggta gggggagttt ttttcatatt tgcaaatagt cgaagttagg
    45241 atttttgaaa gcaaaaggta agtttaaaaa agtttattta gaatgcttct aaattacttg
    45301 acttatttat ttagaattat tataattaat gtatcttaaa gattgaataa cttttaattg
    45361 cgagggaact aaaaatgaca atcagacaat ggatggcaga ccatcttcat ttaatggaga
    45421 ctttagcgtg tttggtatta ataataattg gcctagcctt tttacactca tttccacaag
    45481 tggcttccgc tattttcatt acagccttct tgattggagg atatgcgtct gcaaaaacgg
    45541 gtatattgga tttggtgaag aacaaacact tgtcagtgga tattttgatg attttagcag
    45601 ctatcggtgc tggaattatc ggctattggc tggagggtgc tctgcttatt tttatctttt
    45661 cgttgtccaa tacgcttgaa gaaatggcca tggaaaaaag taaggatgct atttcagcct
    45721 tgatgtcctt gacgccagat acagctcgtc aataccaaga agatggccat attttagaag
    45781 ttgagaccag atccttgagt gttggtgacc gtttacaagt tcgtaaaggc gaagctgttc
    45841 caattgacgg gcagttgctt agtccttttg gtcaatttga tgaatctatg gtcactggtg
    45901 agcctatcac tgttgataag gcagaaggcc aggatctgat tgggggaacc attaaccaag
    45961 gacaaaccat agatatgttg gttactattg aaaatgacga tactctcttt gccaagatta
    46021 ttaatctggt ggaatctgcc caagaaaaga aaagcaaaac tgccaccttt atcgaaagct
    46081 tagaagatgg ttatgtcaaa tttgtgctcg tccttattcc tgcctttatc ctctttagcc
    46141 actttgtgct ttcttggact tggttggctg ctttttaccg agggatgatt ctcttaacag
    46201 tagcttcacc atgtgcccta attgccagtt ccacacctgc tagcttggct gctatttctc
    46261 gtgcagccag aaagggattg attatcaaag gaggggatat tgtcgataac atgggagata
    46321 ttaaggctgt tgtcatggat aaaacgggaa cgctcaccca aggaaaacct tctgttgtga
    46381 atgctcatta tttggaagat gagttgctcg tgaatagact ggtaaaagga gcagagactg
    46441 ctagtaccca tcctatttct aaagcccttc ttgaatacac tgaaaaattg gagccactga
    46501 cctttgacca cttagaagaa atttctggga aaggttttca gggcttctat caagggcaag
    46561 aatggcgaat tggcaagaaa accttcattt tggaaaaggt tcaagaccta tcagcttttg
    46621 aagaaactat tcaagtggaa gaaaatcaag ggaaaaccct aatctttgtt tcacgtgacc
    46681 atcaattgat agcttactat gccctcttgg atgatatcaa aatagaatca aaacgtgcta
    46741 ttaagtctct tcatgccatg ggaatcaaaa cagtcatgtt aacaggtgac caagaacgaa
    46801 ccgccaatta tgtggcacaa aaacttggta ttgatgaagt ggtagccaac tgtatgcctc
    46861 aagataaggt ggctaagtta gcagaattaa agaccaaata tggttttgtg gccatggtag
    46921 gagatggtat taatgatgct cctgcccttg ctcaagcaga tgtttcttat gctattggat
    46981 caggaacaga tattgcaatg gaaagtgcag acagtgtgat tatggatgac ttgactcgta
    47041 ttccattttc gattcaactt tcccgcaaaa tgaagaccat tatcaaacaa aatattgttt
    47101 ttgccttatc tgtgattacc ttattgattt tagccaatgt ttttcaggta gttaacttgc
    47161 cgcttggtgt tgttggacac gaaggctcaa cgattttagt gattttaaat ggcttgcgtt
    47221 tactttcttt taaataattt agaaacttgc ttgaaaactt gaaagtgttt ataataatag
    47281 taagacttat gaaagagaaa accgccgtat gtttactatt atgatcattg gtttagctgt
    47341 attttcaata attaaaatca ttctattttt ctatttttat cagcaatttg agcgcaagca
    47401 aaaaagaaaa gttgaatcaa acaaccagca aagacagtcc caagaaagtg acgctgtccc
    47461 ttcaaaaaaa ttcccatttt aaaaagcttg gaaagtgtac aaagtgcaca tttccaagct
    47521 tttttgacta ttaaggttaa tctttgtaac gtaagttccc acgaaaatcg tctaagcttt
    47581 ggtagccttt ttctaccata atcgttttta gttccttagt gacccgttca aagatagctg
    47641 gcccttcttg atgaagggct gtcccaattt ggaccatgct ggcgccacaa agaatatgtt
    47701 caaaggcatc gcgtcctgtt ttaactcccc ctgtaccaat aatgtggata gaaggtttta
    47761 agcgtttgta aaacgcatgg acattggcta gagcagtagg tttgatgtaa tctccaccta
    47821 taccaccaaa gccattttta ggcttgatga ggacttgttc atcctcaatg accagcccat
    47881 ttccgataga attgacacag ttaacaaagg aaagggggta tttattgaaa atcgcagccg
    47941 cttgatcaaa atgcacaatg tcaaaataag gaggtaattt gataccaaga ggtttagtgt
    48001 aataggtaaa gatgttctct agaagttgat cagtggtttc aaaatcataa gcaatctgtg
    48061 gttttcctgg aacgttaggg caagaaaggt ttagttccac tagaccctca taatctgaag
    48121 ccatgatggc ttttaaaatg gtttcggttt cagtaggaga tagcccgaca acggagagaa
    48181 agtgaggttt atgttgcccg gttttggcaa gatcactgac aaagtctaag tagtagcgaa
    48241 aaccattatt agggagtccc attgaattga tagaaccaag acgagtatct gcgtagcgag
    48301 gttctggatt acctggacga acttcaagcg tccctgtttt ggtgacaaag gaagcggctt
    48361 gagacttttc aacctccata agctcttctt ttgtcatgca gtaaacacca gcagcattca
    48421 tcaaacagtt atcaaaagag aaatgaccaa tttgcgtagc agtagaaacc attatttcct
    48481 ccatatttta gacaaatttt ttctattcta ccactaggca tgaataatag caagctattt
    48541 tcacaaaaat gtaaatatat tactgttatt gacgaacatt tgaaattttc atgatagtca
    48601 cattatgatt ttcaataaag tcagaataga ctagcttttt tgctatattt tttgtaaaat
    48661 aggaattgta aaaaggtagc aacctttaaa ttaaaaggag atattaactc atggtaaaat
    48721 tggttttcgc tcgccacggt gagtcagaat ggaacaaagc taacctcttc actggttggg
    48781 cagatgttga tctttcagaa aaagggacac aacaagcgat tgatgcaggt aaattgatca
    48841 aagaagcagg tattgaattt gaccttgctt tcacttcagt attgacacgt gctatcaaaa
    48901 caactaacct tgcccttgaa aatgcaggtc aattgtgggt tccaactgaa aaatcatggc
    48961 gcttgaacga gcgtcactat ggtgctttga ctggcaaaaa caaagctgaa gctgcagaac
    49021 aatttggtga tgaacaagtt catatctggc gtcgttcata cgacgtattg ccgccagcta
    49081 tggctaaaga tgatgagtat tcagcacaca aagaccgtcg ttatgctgat cttgacccag
    49141 ctcttattcc agatgctgaa aacttaaaag taactcttga acgcgcaatg ccttactggg
    49201 aagaaaaaat tgctccagct cttcttgatg gtaaaaacgt ctttgttggc gcacatggta
    49261 actcaatccg cgctcttgtg aaacacatta aaggtctttc agatgacgaa atcatggatg
    49321 tggaaattcc aaacttccca ccacttgttt ttgaattaga tgaaaaactt aacattgtta
    49381 aagaatacta ccttggtggt gaataactta aaaaccagac tttggtctgg ttttttggtt
    49441 ctatatccta agaagaaaga aaaccctgct taaaaatatg atatgataga aataaaaaag
    49501 gatagataga ttatgaaagc agaagatcgt cgtcaaaaaa ttattgagtg cttaaatagt
    49561 gaacaaaaag ctgtatctgc tactcgttta ggtaagttat taggagtgag tcgccaagta
    49621 atcgttggag atatagctct tcttcgagct cagcagattg atattatttc gactccaaaa
    49681 ggttatatta tgtcaacagc gctttatagt caccaatttt gtgcacgtat tgtgtgccaa
    49741 cataatgtag aagaaactaa aaaagaatta gaaattattt tagctcatca agggattatt
    49801 acgacggtag aggtagaaca tcctatttac ggtatgataa cagccccttt aaatattaag
    49861 acacactctg atgtaactaa tttcatgtcc aaactcagtc aatctaaagc agagttattg
    49921 tcttcgttga cagaaggact acatagtcac ttaatttcgt gtcctagtca agaagccttt
    49981 ttagctatca agcatgattt agaattagca ggtatcttat acaa
//
LOCUS       DATXIG010000008        46430 bp    DNA     linear   BCT 28-DEC-2023
DEFINITION  TPA_asm: Streptococcus pyogenes strain MGAS11113
            SAMN08555799-rid19682583.denovo.008, whole genome shotgun sequence.
ACCESSION   DATXIG010000008 DATXIG010000000
VERSION     DATXIG010000008.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN08555799
            Sequence Read Archive: SRR6740495
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pyogenes
  ORGANISM  Streptococcus pyogenes
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 46430)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 46430)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-OCT-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-OCT-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001918058.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001918058.2
            Genome Coverage       :: 368x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/12/2023 11:23:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 1,806
            CDSs (total)                      :: 1,769
            Genes (coding)                    :: 1,722
            CDSs (with protein)               :: 1,722
            Genes (RNA)                       :: 37
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1, 1 (5S, 23S)
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 47
            CDSs (without protein)            :: 47
            Pseudo Genes (ambiguous residues) :: 0 of 47
            Pseudo Genes (frameshifted)       :: 30 of 47
            Pseudo Genes (incomplete)         :: 20 of 47
            Pseudo Genes (internal stop)      :: 9 of 47
            Pseudo Genes (multiple problems)  :: 11 of 47
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..46430
                     /organism="Streptococcus pyogenes"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN08555799-rid19682583.denovo.008"
                     /strain="MGAS11113"
                     /serotype="Emm28"
                     /isolation_source="normally sterile site"
                     /host="Homo sapiens"
                     /db_xref="taxon:1314"
                     /country="Finland"
                     /collection_date="2002"
                     /collected_by="National Public Health Laboratory"
     gene            160..1392
                     /locus_tag="VC122_000458"
     CDS             160..1392
                     /locus_tag="VC122_000458"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002986011.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanyl-D-alanine carboxypeptidase"
                     /protein_id="HEP5640410.1"
                     /translation="MIKRLISLVVIALFFAASTVSGEEYSVTAKHAIAVDLESGKVLY
                     EKDTKEVVPVASVSKLLTTYLVYKEVSKGKLNWDSPVTISNYPYELTTNYTISNVPLD
                     KRKYTVKELLSALVVNNANSPAIALAEKIGGTEPKFVDKMKKQLRQWGISDAKVVNST
                     GLTNHFLGANTYPNTEPDDENCFCATDLAIIARHLLLEFPEVLKLSSKSSTIFDGQTI
                     YSYNYMLKGMPCYREGVDGLFVGYSKKAGASFVATSVENQMRVITVVLNADQSHEDDL
                     AIFKTTNQLLQYLLINFQKVQLIENNKPVKTLSVLDSPEKTVKLVAQNSLFFIKPIHT
                     KTKNTVHITKKSSTMIAPLSKGQVLGRATLQDKHLIGQGYLDTPPSINLILQKNISKS
                     FFLKVWWNRFVRYVNTSL"
     gene            1560..2741
                     /locus_tag="VC122_000459"
     CDS             1560..2741
                     /locus_tag="VC122_000459"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011017332.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanyl-D-alanine carboxypeptidase"
                     /protein_id="HEP5640411.1"
                     /translation="MTKRIILTIFTFICFSVMPLVQAEDVMDITRQAGYTVSEVNRPK
                     SSIVVDANSSDILWQDNIDIPRDPASMSKMFTLYILFEELAKGKITMDTTITATPTDQ
                     AIANIYEISNNNIVAGVAYPIRDLITMTAVPSSNAATVMIANYLSNNDASAFIDRINA
                     TAKQLGMTNTHFSNASGAAAQAFQGYYNPTKYDLSASNITTARDLSKLLYAFLKKYPE
                     IISFTNKSVVHTMVGTPYEEEFHTYNHSLPDNQFGMKGVDGLKTGSSPSAAFNAMITA
                     KRGKTRLITIVMGVGDWSDQNGEFYRHPFVNALTEKGFKDSKTLSKKARQKLEKLVPQ
                     TKKETSPKQQHFKATKKQSYLERVEDFMNHNHTFLLICLAIFIITILLLSLVVFAMGR
                     Q"
     gene            complement(2893..4311)
                     /gene="sufB"
                     /locus_tag="VC122_000460"
     CDS             complement(2893..4311)
                     /gene="sufB"
                     /locus_tag="VC122_000460"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017769002.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S cluster assembly protein SufB"
                     /protein_id="HEP5640412.1"
                     /translation="MSDINEKVEPKPIDLGDYQFGFHDDVEPIYSTGKGLSEAVVREL
                     SAAKNEPEWMLEFRLKSLETFNKMPMQTWGADLSDINFDDIIYYQKASDKPARSWDDV
                     PEKIKETFDRIGIPEAERAYLAGASAQYESEVVYHNMKGEFEKLGIIFTDTDSALKEY
                     PDLFKQYFAKLVPPTDNKLAALNSAVWSGGTFIYVPKGVKVDIPLQTYFRINNENTGQ
                     FERTLIIVDEGASVHYVEGCTAPTYSSNSLHAAIVEIFALDGAYMRYTTIQNWSDNVY
                     NLVTKRARALTDATVEWIDGNLGAKTTMKYPSVYLDGPGARGTMLSIAFANAGQHQDT
                     GAKMIHNAPHTSSSIVSKSIAKSGGKVDYRGQVTFNKQSKKSVSHIECDTILMDDISK
                     SDTIPFNEIHNSQVALEHEAKVSKISEEQLYYLMSRGLSESEATEMIVMGFVEPFTKE
                     LPMEYAVELNRLISYEMEGSVG"
     gene            complement(4304..4783)
                     /locus_tag="VC122_000461"
     CDS             complement(4304..4783)
                     /locus_tag="VC122_000461"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002986018.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SUF system NifU family Fe-S cluster assembly
                     protein"
                     /protein_id="HEP5640413.1"
                     /translation="MALSKLNHLYMAVVADHSKRPHHHGQLDGVEAVQLNNPTCGDVI
                     SLTVKFDEDKIEDIAFAGNGCTISTASSSMMTDAVIGKSKEEALALADIFSEMVQGQE
                     NPAQKELGEAELLAGVAKFPQRIKCSTLAWNALKEAIKRSANAQHLTDQNVKEGKNV"
     gene            complement(4770..5996)
                     /locus_tag="VC122_000462"
     CDS             complement(4770..5996)
                     /locus_tag="VC122_000462"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002986020.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine desulfurase"
                     /protein_id="HEP5640414.1"
                     /translation="MLDAKDIKQDFQILNQQVNDEPLVYLDNAATTQKPALVLEALQS
                     YYQEDNANVHRGVHTLAERATHKYEASRQQVADFIHAKSSKEVLFTRGTTTSLNWVAR
                     FAEQVLTPEDEVLISIMEHHANIIPWQQACQKTGARLVYVYLKDGQLDMDDLANKLTT
                     KTRFVSLVHVSNVLGCINPIKEIAKLAHAKGAYLVVDGAQSVPHLAIDVQDLDCDFFA
                     FSAHKMLGPTGLGVLYGKEELLNQVEPLEFGGEMIDFVYEQEATWKELPWKFEAGTPH
                     IAGAIGLSAAISYLQRLGMADIHAHEAELIAYVLPKLEAIEGLTIYGPSQPSARSGLI
                     SFNLDDLHPHDLATALDYEGVAVRAGHHCAQPLLSYLGVPATVRASFYIYNTKADCDR
                     LVEAILKAKEFFNGTL"
     gene            complement(6027..7289)
                     /gene="sufD"
                     /locus_tag="VC122_000463"
     CDS             complement(6027..7289)
                     /gene="sufD"
                     /locus_tag="VC122_000463"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014407299.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S cluster assembly protein SufD"
                     /protein_id="HEP5640415.1"
                     /translation="MTKEKLVAFSQAHAEPAWLQERRLAALEAIPELELPTIERVKFH
                     RWNLGDGTLTENESLASVPDFIAIGDNPKLVQVGTQTVLEQLPMALIDKGVVFSDFYT
                     ALEEIPEVIEAHFGQALAFDEDKLAAYHTAYFNSAAVLYVPDHLEITTPIEAIFLQDS
                     DSDVPFNKHVLVIAGKESKFTYLERFESIGNATQKTSANISVEVIAQAGSQIKFSAID
                     RLGPSVTTYISRRGRLEKDANIDWALAVMNEGNVIADFDSDLIGQGSQADLKVVAASS
                     GRQVQGIDTRVTNYGQRTVGHILQHGVILERGTLTFNGIGHILKGAKGADAQQESRVL
                     MLSDQARADANPILLIDENEVTAGHAASIGQVDPEDMYYLMSRGLDQETAERLVIRGF
                     LGAVIAEIPIPSVHQEIIKVLDEKLLNR"
     gene            complement(7384..8154)
                     /gene="sufC"
                     /locus_tag="VC122_000464"
     CDS             complement(7384..8154)
                     /gene="sufC"
                     /locus_tag="VC122_000464"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002986023.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S cluster assembly ATPase SufC"
                     /protein_id="HEP5640416.1"
                     /translation="MSILEINNLHVSIEGKEILKGVNLTLKTGEVAAIMGPNGTGKST
                     LSAAIMGNPNYEVTQGQILLDGVNILDLEVDERARLGLFLAMQYPSEIPGITNAEFMR
                     AAMNAGKADKDKISVRDFITKLDEKMALLGMKEEMAERYLNEGFSGGEKKRNEILQLL
                     MLEPKFALLDEIDSGLDIDALKVVSKGVNEMRGKDFGAMIITHYQRLLNYITPDLVHV
                     MMDGRIVLSGDAALATRLEKEGYAGIAQDLGIEYKEES"
     gene            complement(8276..9445)
                     /locus_tag="VC122_000465"
     CDS             complement(8276..9445)
                     /locus_tag="VC122_000465"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014407298.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="undecaprenyl/decaprenyl-phosphate
                     alpha-N-acetylglucosaminyl 1-phosphate transferase"
                     /protein_id="HEP5640417.1"
                     /translation="MFSFTIDYVLVLIGALLMSLFLTPLVRFLAFRVGAVDNPNARRV
                     NKVPMPTSGGLAIFMSFLVASLGLIPIASKGAMFFGQTYFSYILPVVIGATVITLTGF
                     LDDLYELSPKLKMFGILIGAVIVWAFTDFKFDSFKIPFGGPLLVFGPFLTLFLTVLWI
                     VSITNAINLIDGLDGLVSGVSIISLVTMAIVSYFFLPQKDFFLTLTILVLISAIAGFF
                     PYNYHPAMIYLGDTGALFIGFMIGVLSLQGLKNSTAVAVVTPVIILGVPIMDTIVAII
                     RRSLSGQKFYEPDKMHLHHRLLSMGFTHRGAVLVVYGITMLFSLISLLLNVSSRIGGV
                     LLMLGLLFGLEVFIEGLEIWGEKRTPLFNLLKFIGNSDYRQAMLLKWKEKKDLKH"
     gene            complement(9452..10213)
                     /gene="mecA"
                     /locus_tag="VC122_000466"
     CDS             complement(9452..10213)
                     /gene="mecA"
                     /locus_tag="VC122_000466"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003059809.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adaptor protein MecA"
                     /protein_id="HEP5640418.1"
                     /translation="MEMKQISETTLKITISMDDLEERGMELKDFLIPQEKTEEFFYSV
                     MDELDLPDNFKDSGMLSFRVTPRKDRLDVFVTKSEINKDINLEDLAEFGDMSQMTPED
                     FFKSLEQSMREKGDVKAHEKLEKIEEIMEDVVEATLANQSEAADPSTNHESEPLDYVH
                     YVLDFSTITEAVAFAKTIDFSIEASELYKGSNCYHMTILLDVQQQPSYFANVMYARLI
                     EHANPGSKTRAYLQEHGLQLMLDGAVEQLQKIELG"
     gene            complement(10359..11198)
                     /locus_tag="VC122_000467"
     CDS             complement(10359..11198)
                     /locus_tag="VC122_000467"
                     /EC_number="3.6.1.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015057324.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="undecaprenyl-diphosphate phosphatase"
                     /protein_id="HEP5640419.1"
                     /translation="MLIIELLKAIFFGIIEGITEWLPVSSTGHLILVQEFIRLNQDKA
                     FIEMFNIVIQLGAIIAVMLIYFERLNPFQPGKTAREVQLTWQLWLKVVIACIPSILIA
                     VPLDNWFEAHFYFMVPIAIALIVYGIAFIWIEKRNAQQEPAVTELARMSYKTAFFIGC
                     FQVLSIVPGTSRSGATILGAIILGTSRTVAADFTFFLAIPTMFGYSGLKAVKFFLDGH
                     HLDFAQVLILLVASLTAFVVSLLAIRFLTDYVKKHDFTIFGKYRIVLGSLLLIYSFFK
                     FVF"
     gene            complement(11264..13189)
                     /locus_tag="VC122_000468"
     CDS             complement(11264..13189)
                     /locus_tag="VC122_000468"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014407295.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2207 domain-containing protein"
                     /protein_id="HEP5640420.1"
                     /translation="MKKILMTLVLCFSLLGIRIKAADVDYSITNYEGQLLLSKENTAR
                     FEQKVTYQFDTSYNGQYISLGRAGHLPAGFAIDQKPKVEVYQNGQQVPVSQEFSDLGD
                     GYRLKLYNAGQAGDKVDVKVIWQLHHLLTAYQDVAELNWTPISDWDKTLEKVSLTVTT
                     PTDIQDSNLWAHRGYYQKKPQVLKEGNSRYQINAKNVSGQLELHAYWDKKALLGEEPV
                     DVSTSKKNKIVALETKISRRRALLQLLFGKVIPLVEVGFLLWQLIQFIRLKKQFNRYH
                     LANHTDHSYEVPEDLSPLVLTQAIYGQSFAYLSPTASESQKLLIPKGVTFEALVQATL
                     LDLIDQKVLLLTKEEGKAYLEISQLDRVTDEEAAFLDMAFGNKVTLPVDQLFSQYHYD
                     ADTIKQLKKTYKGKKLEQEVRQSSEQVIKAMKKASAAITNNVLETIKKLNLPDTYRQM
                     TPAEKRKSNSVQGLGCLLLVLNSGLLIYLAIKESGLALIYLALMVLTMCLGFYISLKL
                     DQYKKLGIETPEGGVRLHQWQSFKNMIRDIDKFEDVAIEGLVVWNRVLVYATLFGYAK
                     KVERYLKVHRIALPEVYQAVRPGELSMVMYATTPTFLSSLSSATTSSNFSVSSGGGIS
                     GGGGFSGGGGGGGGGAF"
     gene            13387..14955
                     /locus_tag="VC122_000469"
     CDS             13387..14955
                     /locus_tag="VC122_000469"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011285384.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding
                     protein/permease"
                     /protein_id="HEP5640421.1"
                     /translation="MKKLILSCLVALALLFGGMSRAQANQYLRVGMEAAYAPFNWTQD
                     DASNGAVPIEGTSQYANGYDVQVAKKVAKAMNKELLVVKTSWTGLIPALTSGKIDMIA
                     AGMSPTKERRNEISFSNSYYTSQPVLVVTANGKYADATSLKDFSGAKVTAQQGVWHVN
                     LLTQLKGAKLQTPMGDFSQMRQALTSGVIDAYISERPEAMTAEAADSRLKMITLKKGF
                     AVAESDAAIAVGMKKNDDRMATVNQVLEGFSQTDRMALMDDMVTKQPVEKKAEDAKAS
                     FLGQMWAILKGNWKQFLRGTGMTLLISMVGTITGLFIGLLIGIFRTAPKAKHKVAALG
                     QKLFGWLLTIYIEIFRGTPMIVQSMVIYYGTAQAFGISIDRTLAAIFIVSINTGAYMS
                     EIVRGGIFAVDKGQFEAATALGFTHGQTMRKIVLPQVVRNILPATGNEFVINIKDTSV
                     LNVISVVELYFSGNTVATQTYQYFQTFTIIAIIYFVLTFTVTRILRHIERRFDADTYT
                     TGANQMQIAEVSNV"
     gene            14948..15688
                     /locus_tag="VC122_000470"
     CDS             14948..15688
                     /locus_tag="VC122_000470"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003054906.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter ATP-binding protein"
                     /protein_id="HEP5640422.1"
                     /translation="MSNSIIEIKNLKKSYGSNEVLKDISLSVNKGEVISIIGSSGSGK
                     STLLRSINLLEEPSAGQILFHGEDVLAEHYNLTHYREKLGMVFQSFNLFENLNVLENA
                     IVAQTTVLKRDRAQAEQIAKENLNAVGMTEQYWQAKPKQLSGGQKQRVAIARALSVNP
                     EAMLFDEPTSALDPEMVGEVLKTMQDLAKSGLTMIIVTHEMEFARDVSDHIIFMDKGL
                     ITEEGSPQQIFENPTQDRTKEFLQRFLK"
     gene            15830..15946
                     /locus_tag="VC122_000471"
     CDS             15830..15946
                     /locus_tag="VC122_000471"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000818732.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative metal homeostasis protein"
                     /protein_id="HEP5640423.1"
                     /translation="MAEKTDLSSAYRRLKSPNIKTRKRALKIIHEYKRYGKK"
     gene            complement(16172..17182)
                     /gene="gap"
                     /locus_tag="VC122_000472"
     CDS             complement(16172..17182)
                     /gene="gap"
                     /locus_tag="VC122_000472"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008090210.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I glyceraldehyde-3-phosphate dehydrogenase"
                     /protein_id="HEP5640424.1"
                     /translation="MVVKVGINGFGRIGRLAFRRIQNIEGVEVTRINDLTDPNMLAHL
                     LKYDTTQGRFDGTVEVKEGGFEVNGNFIKVSAERDPENIDWATDGVEIVLEATGFFAK
                     KEAAEKHLHANGAKKVVITAPGGNDVKTVVFNTNHDILDGTETVISGASCTTNCLAPM
                     AKALHDAFGIQKGLMTTIHAYTGDQMILDGPHRGGDLRRARAGAANIVPNSTGAAKAI
                     GLVIPELNGKLDGAAQRVPVPTGSVTELVVTLDKNVSVDEINAAMKAASNDSFGYTED
                     PIVSSDIVGVSYGSLFDATQTKVMEVDGSQLVKVVSWYDNEMSYTAQLVRTLEYFAKI
                     AK"
     gene            complement(17530..19608)
                     /gene="fusA"
                     /locus_tag="VC122_000473"
     CDS             complement(17530..19608)
                     /gene="fusA"
                     /locus_tag="VC122_000473"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000090325.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor G"
                     /protein_id="HEP5640425.1"
                     /translation="MAREFSLAKTRNIGIMAHVDAGKTTTTERILYYTGKIHKIGETH
                     EGASQMDWMEQEQERGITITSAATTAQWDGHRVNIIDTPGHVDFTIEVQRSLRVLDGA
                     VTVLDSQSGVEPQTETVWRQATEYGVPRIVFANKMDKIGADFLYSVQTLHDRLQANAH
                     PIQLPIGAEDDFRGIIDLIKMKAEIYTNDLGTDILEEDIPEEYLEQAQEYREKLIEAV
                     AETDEDLMMKYLEGEEITNDELIAGIRKATINVEFFPVLCGSAFKNKGVQLMLDAVIA
                     YLPSPLDIPAIKGVNPDTDAEEERPASDEEPFAALAFKIMTDPFVGRLTFFRVYSGVL
                     NSGSYVMNTSKGKRERIGRILQMHANSRQEIETVYAGDIAAAVGLKDTTTGDSLTDEK
                     AKVILESIEVPEPVIQLMVEPKSKADQDKMGVALQKLAEEDPTFRVETNVETGETVIA
                     GMGELHLDVLVDRMKREFKVEANVGAPQVSYRETFRASTQARGFFKRQSGGKGQFGDV
                     WIEFTPNEEGKGFEFENAIVGGVVPREFIPAVEKGLIESMANGVLAGYPMVDVKAKLY
                     DGSYHDVDSSETAFKIAASLALKEAAKSAQPAILEPMMLVTITAPEDNLGDVMGHVTA
                     RRGRVDGMEAHGNSQIVRAYVPLAEMFGYATVLRSATQGRGTFMMVFDHYEDVPKSVQ
                     EEIIKKNKGE"
     gene            complement(19975..20445)
                     /gene="rpsG"
                     /locus_tag="VC122_000474"
     CDS             complement(19975..20445)
                     /gene="rpsG"
                     /locus_tag="VC122_000474"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011285383.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S7"
                     /protein_id="HEP5640426.1"
                     /translation="MSRKNQAPKREVLPDPLYNSKIVTRLINRVMLDGKRGTAATIVY
                     DAFSAIKEATGNDALEVFETAMDNIMPVLEVRARRVGGSNYQVPVEVRPERRTTLGLR
                     WLVNASRARGEHTMKDRLAKEIMDAANNTGASVKKREDTHKMAEANRAFAHFRW"
     gene            complement(20466..20879)
                     /gene="rpsL"
                     /locus_tag="VC122_000475"
     CDS             complement(20466..20879)
                     /gene="rpsL"
                     /locus_tag="VC122_000475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001142328.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S12"
                     /protein_id="HEP5640427.1"
                     /translation="MPTINQLVRKPRKSKIEKSDSPALNIGYNSHKKVQTKMAAPQKR
                     GVATRVGTMTPKKPNSALRKFARVRLSNLIEVTAYIPGIGHNLQEHSVVLIRGGRVKD
                     LPGVRYHIVRGALDTAGVADRKQGRSKYGAKRPKG"
     gene            complement(21087..23708)
                     /locus_tag="VC122_000476"
     CDS             complement(21087..23708)
                     /locus_tag="VC122_000476"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002991142.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SEC10/PgrA surface exclusion domain-containing
                     protein"
                     /protein_id="HEP5640428.1"
                     /translation="MDLEQTKPNQVKQKIALTSTIALLSASVGVSHQVKADDRASGET
                     KASNTHDDSLPKPETIQEAKATIDAVEKTLSQQKAELTELATALTKTTAEINHLKEQQ
                     DNEQKALTSAQEIYTNTLASSEETLLAQGAEHQRELTATETELHNAQADQHSKETALS
                     EQKASISAETTRAQDLVEQVKTSEQNIAKLNAMISNPDAITKAAQTANDNTKALSSEL
                     EKAKADLENQKAKVKKQLTEELAAQKAALAEKEAELSRLKSSAPSTQDSIVGNNTMKV
                     PQGYPLEELKKLEASGYIGSASYNNYYKEHADQIIAKASPGNQLNQYQDIPADRNRFV
                     DPDNLTPEVQNELAQFAAHMINSVRRQLGLPPVTVTAGSQEFARLLSTSYKKTHGNTR
                     PSFVYGQPGVSGHYGVGPHDKTIIEDSAGASGLIRNDDNMYENIGAFNDVHTVNGIKR
                     GIYDSIKYMLFTDHLHGNTYGHAINFLRVDKRNPNAPVYLGFSTSNVGSLNEHFVMFP
                     ESNIANHQRFNKTPIKAVGSTKDYAQRVGTVSDTIAAIKGKVSSLENRLSAIHQEADI
                     MAAQAKVSQLQGKLASTLKQSDSLNLQVRQLNDTKGSLRTELLAAKAKQAQLEATRDQ
                     SLAKLASLKAALHQTEVLAEQAAARVTALVAKKAHLQYLRDFKLNPNRLQVIRERIDN
                     TKQDLAKTTSSLLNAQETLAALQAKQSSLEATIATTEHQLTLLKTLANEKGYRHLDED
                     IATVPDLQVAPPLTGVKPLSYSKIDTTPLVQEMVKETKQLLEASARLAAENTSLVAEA
                     LVGQTSEMVASNAIVSKITSSITQPSSKTSYGSGSSTTSNLISDVDESTQRALKAGVV
                     MLAAVGLTGFRFRKESK"
     gene            complement(23699..24538)
                     /gene="purR"
                     /locus_tag="VC122_000477"
     CDS             complement(23699..24538)
                     /gene="purR"
                     /locus_tag="VC122_000477"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009880602.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pur operon repressor"
                     /protein_id="HEP5640429.1"
                     /translation="MKLRRSERMVVISNYLINNPYKLTSLNTFATKYEAAKSSISEDI
                     AIIKKAFEEANIGDIDTLTGASGGVIFTPSISETEARTIVEDLCQRLSESDRILPGGY
                     IYLSDLLSTPKILQNIGRIIANAFKGEKIDAVMTVATKGVPLANAVANILSVPFVIVR
                     RDLKITEGSTVSVNYASASSDRIEKMFLSKRSLKPNSRVLIVDDFLKGGGTITGMISL
                     LTEFDSTLVGVAVFAENAQSEREQMTFKSLLKVSEIDVKNNNVVVEVGNIFDKWKKER
                     EWT"
     gene            complement(24805..25743)
                     /locus_tag="VC122_000478"
     CDS             complement(24805..25743)
                     /locus_tag="VC122_000478"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003045335.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3'-5' exoribonuclease YhaM family protein"
                     /protein_id="HEP5640430.1"
                     /translation="MKINQMKKDQLFEGFYLIKSAEVRKTRAGKDFISLTFQDDTGEI
                     SGNLWDAQPYNVEEFTAGKVVFMKGRREVYNGTPQVNQITLRNVRPGEPNDPKDFKEK
                     APVSVTEVRDYLEQMLFKIENATWQRIVRALYRKYDKEFYTYPAAKTNHHAFESGLAY
                     HTATMVRLADSIGDIYPDLNKSLLFAGIMLHDLAKVIELTGPDNTEYTVRGNLIGHIS
                     LINEEITKVISELQIDDTKEEVIVLRHVILSHHGQLEYGSPVRPRIMEAEIIHMIDNI
                     DANMMMMTTALSRVSEGEMTNRIFAMDNRSFYKPNY"
     gene            complement(25733..27004)
                     /locus_tag="VC122_000479"
     CDS             complement(25733..27004)
                     /locus_tag="VC122_000479"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014407291.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA recombination protein RmuC"
                     /protein_id="HEP5640431.1"
                     /translation="MDLILFLLVLVLLGLGAYLLFKVNGLQHQLAQTLEGNADNLSDQ
                     ITYQLDTANKQQLLELTQLMNRQQADLYQQLTDIRDVLHRSLSDSRDRSDKRLEEINQ
                     QVNQSLKNMQESNEKRLEEMRQIVEEKLEETLKNRLHASFDSVSKQLESVNKGLGEMR
                     SVAQDVGTLNKVLSNTKTRGILGELQLGQIIEDIMTSSQYEREFVTVSGSSERVEYAI
                     KLPGNGQGGYIYLPIDSKFPLEDYYRLEDAYEVGDKLAIEASRKALLAAIKRFAKDIH
                     KKYLNPPETTNFGVMFLPTEGLYSEVVRNAAFFDSLRREENIVVAGPSTLSALLNSLS
                     VGFKTLNIQKNADDISKILGNVKLEFDKFGGLLAKAQKQMNTANNTLDQLISTRTNAI
                     VRALNTVETYQDQTTKSLLNMPLLEEENNEN"
     gene            complement(27006..27638)
                     /locus_tag="VC122_000480"
     CDS             complement(27006..27638)
                     /locus_tag="VC122_000480"
                     /EC_number="2.7.6.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010921882.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine diphosphokinase"
                     /protein_id="HEP5640432.1"
                     /translation="MSKVALFAGGDLSYISRDFDYFVGIDRGSLFLLENGLPLNMAVG
                     DFDSVSQKAFTDIKEKAELFITAHPEKNDTDTELALKEVFARFPEAEVTIFGAFGGRM
                     DHLLSNIFLPSDPGIAPFMAQIALRDQQNMITYRPAGQHLIHQEEGMTYVAFMAEGEA
                     DLTITGAKFELTQDNFFKKKIYSSNAFIHQPITVSLPSGYLIIIQSKDWS"
     gene            complement(27631..28293)
                     /locus_tag="VC122_000481"
     CDS             complement(27631..28293)
                     /locus_tag="VC122_000481"
                     /EC_number="5.1.3.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002992061.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribulose-phosphate 3-epimerase"
                     /protein_id="HEP5640433.1"
                     /translation="MSTLKIAPSILAADYANFASELARIEETDAEYVHIDIMDGQFVP
                     NISFGADVVASMRKHSKLVFDCHLMVVDPERYVEAFAQAGVDIMTIHTESTRHIHGAL
                     QKIKAAGMKAGVVINPGTPATALEPLLDLVDQVLIMTVNPGFGGQAFIPECLEKVATV
                     AKWRDEKGLSFDIEVDGGVDNKTIRACYEAGANVFVAGSYLFKASDLVSQVQTLRTAL
                     NV"
     gene            complement(28303..29175)
                     /gene="rsgA"
                     /locus_tag="VC122_000482"
     CDS             complement(28303..29175)
                     /gene="rsgA"
                     /locus_tag="VC122_000482"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014635274.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome small subunit-dependent GTPase A"
                     /protein_id="HEP5640434.1"
                     /translation="MQGKIIKSLAGFYYVESEGQVYQTRARGNFRKRGETPYVGDIVD
                     FSAEDNSEGYILAIHPRKNSLVRPPIVNIDQAVVIMSAKEPEFNSNLLDRFLILLEHK
                     AIHPVVYISKMDLLDSPEEIKAIGRQYQAIGYDFVTSLEELLPLLADKITVFMGQTGV
                     GKSTLLNRIAPELALETGEISDSLGRGRHTTRAVSFYNTHGGKIADTPGFSSLDYDIA
                     NAEDLNEAFPELRRLSHECKFRSCTHTHEPKCAVKAALETGELWPVRYEHYLQFLSEI
                     ENRRETYKKVIKRK"
     gene            complement(29599..30471)
                     /gene="rsmA"
                     /locus_tag="VC122_000483"
     CDS             complement(29599..30471)
                     /gene="rsmA"
                     /locus_tag="VC122_000483"
                     /EC_number="2.1.1.182"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008535644.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA
                     (adenine(1518)-N(6)/adenine(1519)-N(6))-
                     dimethyltransferase RsmA"
                     /protein_id="HEP5640435.1"
                     /translation="MRIADYSVTKAVLDRHGFTFKKSFGQNFLTDTNILQKIVDTAEI
                     DQNVNVIEIGPGIGALTEFLAENAAEVMAFEIDDRLVPILADTLRDFDNVQVVNQDIL
                     KADLQTQIKQFKNPDLPIKVVANLPYYITTPILMHLIESKIPFQEFVVMMQREVADRI
                     SAEPNTKAYGSLSIAVQYYMTAKVAFIVPRTVFVPAPNVDSAILKMVRRDQPLIEVKD
                     EDFFFRVSRLSFVHRRKTLWNNLTSHFGKSEDIKTKLEKGLALADIKPSIRGEALSIQ
                     DFGKLADALKEVGL"
     gene            complement(30585..31175)
                     /gene="rnmV"
                     /locus_tag="VC122_000484"
     CDS             complement(30585..31175)
                     /gene="rnmV"
                     /locus_tag="VC122_000484"
                     /EC_number="3.1.26.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011285382.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease M5"
                     /protein_id="HEP5640436.1"
                     /translation="MQRGYLSLTEKINIQEVLVVEGKDDTANLRRFYEVDTYETRGSA
                     ITEEDLERINRLNDLRGVIVLTDPDYNGERIRKLIMAAVPTARHAFLNRNEAVPSSKS
                     KGRSLGVEHANFEDLQKALAHVTQQYDDESYFDIRQTDLIRLGLLMASDSRKRREYLG
                     EKLRIGYANGKQLLKRLELFGITLAEVEEVMETYEE"
     gene            complement(31147..31938)
                     /locus_tag="VC122_000485"
     CDS             complement(31147..31938)
                     /locus_tag="VC122_000485"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002986070.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TatD family hydrolase"
                     /protein_id="HEP5640437.1"
                     /translation="MTNKVEKLTIFDTHTHLNVAEFQGHETEELTLAQEMGVAYHNVV
                     GFDQATISGALALADQYPNVYATIGWHPTEAGSYSEAVEESIVSQLSHPKVIALGEIG
                     LDYYWMEDPKEVQIEVFKRQMQLAKDHDLPFVVHTRDALEDTYEVIKAAGVGPRGGIM
                     HSYSGSLEMAERFIELGMMISFSGVVTFKKALDIQEAAQHLPLDKILVETDAPYLTPV
                     PKRGKQNHTAYTRYVVDKIAELRGMTVEEVAKETTANAKRVLKLD"
     gene            32223..33053
                     /locus_tag="VC122_000486"
     CDS             32223..33053
                     /locus_tag="VC122_000486"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010921879.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MurR/RpiR family transcriptional regulator"
                     /protein_id="HEP5640438.1"
                     /translation="MPKNHRLITKIEASLEHMTSLEKGIAHFFITTDLTPQELTASEI
                     VKRLHISQAALTRFAKKCGFTGYRAFAFDYLHSLQESQETFQSIHLELTKRVLMDYDA
                     LINKTYELVNEEKLLNLAKLIDASERVYFFGKGSSGLVAREMKLRFMRLGLICDAYSD
                     TDGFTWANSLVNENCLVFGFSLSGKTNSVITALHQASQRGAKTVLLTTDNQTEFDDSL
                     DIIPVSSTHQLHYGNRVSPQFPLLIMMDIIYAYVLAIDKPHKEKIFKNTIIDKENNGL
                     "
     gene            complement(33164..34102)
                     /locus_tag="VC122_000487"
     CDS             complement(33164..34102)
                     /locus_tag="VC122_000487"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011528232.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ROK family protein"
                     /protein_id="HEP5640439.1"
                     /translation="MKHYLAIDIGGTAIKYGLISETGDLLEKEEMATEAYKGGPSILE
                     KVKGLVKTYQDQMDLAGVAISSAGMVNPDKGEIFYAGPQIPNYAGTQFKKEIEETFGL
                     PCEVENDVNCAGLAEAISGSAKDYPVALCLTIGTGIGGCLLFNSQVFHGSSHSACEVG
                     YLHLSDGQFQDLASTTALVQEVALAYGDDISQWDGRRIFEQAKAGDAICIAAISKQVD
                     YLGQGIANICYVVNPNVVVLGGGIMVQKDYLADKLKTALDSYLVSSLAKKTQLKFASH
                     GNNAGILGAYYHFKQKNERSCSFITLEKIENNLASS"
     gene            complement(34124..35038)
                     /locus_tag="VC122_000488"
     CDS             complement(34124..35038)
                     /locus_tag="VC122_000488"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002894436.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrodipicolinate synthase family protein"
                     /protein_id="HEP5640440.1"
                     /translation="MTDLTKYQGIIPAFYACYDDQGAISPERVRALTQYYIDKGVQGL
                     YINGSSGECIYQSVFDRQLVLENVMAVAKGKLTIINHVACNNTKDSIELAAHSERLGV
                     DAIAAIPPIYFRLPEYAVADYWNAISSAAPHTDFIIYNIPQLAGVALTPSLYKTMLAN
                     KRVIGVKNSSMPVQDIQTFCAIGGDDHIVFNGPDEQFLGGRLMGAAAGIGGTYGAMPE
                     LFLRLNQLIVDKDLEKAKTLQYTINETIGVLVSAHGNMYGVIKEVLRINEGLDIGSVR
                     SPLAELVEEDRVICQRAAALINQAKETF"
     gene            complement(35050..35712)
                     /locus_tag="VC122_000489"
     CDS             complement(35050..35712)
                     /locus_tag="VC122_000489"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010921876.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YesL family protein"
                     /protein_id="HEP5640441.1"
                     /translation="MTSKKQGLLHSLFKLDSKWMRASAALFDLLVFNLLFVLSCLPLL
                     TIGVAKMALYASLLDWREGQVSQLVTTYSSHFKHYFKSGMRLGLIELLIMSICVLDLF
                     LIRNQSGLVFQGFKVLCIAVLFLVVILFLYAYPQAVRKELPLLILFKRSLLLAGILFP
                     WTFAFLVVIGLLVFTLPLTLLTLFCGLSLLATMGISSLTYLYLIIMESLLRRFPLNND
                     IE"
     gene            complement(35869..36699)
                     /locus_tag="VC122_000490"
     CDS             complement(35869..36699)
                     /locus_tag="VC122_000490"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017767339.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HEP5640442.1"
                     /translation="MTKKKLTASDVLTTVILCILTILFIFPFYWIMTGAFKAQADTIM
                     IPPQWWPKAPTIENFKALVVQNPALKWLWNSVFISVATMFLVCGTSSLAGYALAKKRF
                     YGQRLLFSIFIAAMALPKQVVLVPLVRIVNFMGIHDTLAAVILPLVGWPFGVFLMKQF
                     SENIPTELLESAKIDGCGEIRTFFNVAFPIVKPGFAALAIFTFINTWNDYFMQLVMLT
                     SRENLTISLGVATMQAEMATNYGLIMAGAAMAAVPIVTVFLVFQKSFTQGITMGAVKG
                     "
     gene            complement(36712..37599)
                     /locus_tag="VC122_000491"
     CDS             complement(36712..37599)
                     /locus_tag="VC122_000491"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001260031.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HEP5640443.1"
                     /translation="MNVNKLKMRETLISYAFLAPVLVFFVIFVLIPMIMGFVTSFFNY
                     SMTDFTFVGFANYARMFQDPIFMKSLINTLIIVIGSVPVVVFFSLFVAAKTYDKNVVA
                     RSFYRAVFFLPVVTGSVAVTVVWKWIYDPMSGILNYVLKYAHVIEQNISWLGDKHWAL
                     LAIIAILLTTSVGQPIILYIAAMGNIDNSLMEAARVDGATEFQVFWNIKWPSLLPTTL
                     YIAVITTINSFQCFALIQLLTSGGPNYSTSTLMYYLYEKAFKLSEYGYANTMGVFLAV
                     MIAIISFAQFKILGNDVEY"
     gene            complement(37702..39021)
                     /locus_tag="VC122_000492"
     CDS             complement(37702..39021)
                     /locus_tag="VC122_000492"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011528226.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter substrate-binding
                     protein"
                     /protein_id="HEP5640444.1"
                     /translation="MNMKKLASLVMLGASVLGLAACGGKSQKEAGASKSDTAKTEITW
                     WAFPVFTQEKAEDGVGTYEKKLIAAFEKANPEIKVKLETIDFTSGPEKITTAIEAGTA
                     PDVLFDAPGRIIQYGKNGKLADLNDLFTEEFTKDVNNDKLIQASKAGDTAYMYPISSA
                     PFYMALNKKMLKDAGVLDLVKEGWTTDDFEKVLKALKDKGYNPGSFFANGQGGDQGPR
                     AFFANLYSSHITDDKVTKYTTDDANSIKAMTKISNWIKDGLMMNGSQYDGSADIQNFA
                     NGQTSFTILWAPAQPGIQAKLLEASKVDYLEIPFPSDDGKPELEYLVNGFAVFNNKDE
                     QKVAASKTFIQFIADDKEWGPKNVVRTGAFPVRTSYGDLYKDKRMEKIAEWTKFYSPY
                     YNTIDGFAEMRTLWFPMVQAVSNGDEKPEDALKAFTEKANKTIKKTQ"
     gene            complement(39070..39774)
                     /locus_tag="VC122_000493"
     CDS             complement(39070..39774)
                     /locus_tag="VC122_000493"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014407284.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmannosamine-6-phosphate 2-epimerase"
                     /protein_id="HEP5640445.1"
                     /translation="MPDKPTKEKLMEQLKGGIIVSCQALPGEPLYSETGGIMPLLAKA
                     AQEAGAVGIRANSVRDIKEIQAITDLPIIGIIKKDYPPQEPFITATMAEVDQLAALNI
                     AVIAMDCTKRDRHDGLDIASFIRQVKEKYPNQLLMADISTFDEGLVAHQAGIDFVGTT
                     LSGYTPYSRQEAGPDVALIEALCKAGIAVIAEGKIHSPEEAKKINDLGVAGIVVGGAI
                     TRPKEIAERFIEALKS"
     gene            complement(40048..40182)
                     /gene="rpmH"
                     /locus_tag="VC122_000494"
     CDS             complement(40048..40182)
                     /gene="rpmH"
                     /locus_tag="VC122_000494"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000831903.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L34"
                     /protein_id="HEP5640446.1"
                     /translation="MKRTYQPSKIRRQRKHGFRHRMSTKNGRRVLAARRRKGRKVLSA
                     "
     gene            complement(40497..41411)
                     /locus_tag="VC122_000495"
     CDS             complement(40497..41411)
                     /locus_tag="VC122_000495"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015058067.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein jag"
                     /protein_id="HEP5640447.1"
                     /translation="MVLFTGKTVEEAIETGLQELGLSRLKAHIKVISKEKKGFLGFGK
                     KPAQVDIEGISDKTVYKADKKATRGVPEDINQKNAPAVNSADVEPEEIKATQKLEAED
                     TKVVPLMSEDSPAQTLSNLAETVTETKAQQPSIPVEESEVPQDPGNDGFSKDIEKAAQ
                     EVSDYVTKIIYEMDIEATVETSNNRRQINLQIETPEAGRVIGYHGKVLKSLQLLAQNF
                     LHDRYSKNFSVSLNVHDYVEHRTETLIDFTQKVAKRVLESGQDYTMDPMSNSERKIVH
                     KTVSSIEGVDSYSEGNDPNRYVVVSLQR"
     gene            complement(41423..42250)
                     /locus_tag="VC122_000496"
     CDS             complement(41423..42250)
                     /locus_tag="VC122_000496"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011184147.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="membrane protein insertase YidC"
                     /protein_id="HEP5640448.1"
                     /translation="MRKVLRVKKNIKIARIVPLVLLLVACGRGEVTAQSSSGWDQLVY
                     LFARAIQWLSFDGSIGVGIILFTLTIRLMLMPLFNMQIKSSQKMQDIQPELRELQKKY
                     AGKDTQTRMKLAEESQALYKKYGVNPYASLLPLLIQMPVMIALFQALTRVSFLKTGTF
                     LWVELAQHDHLYLLPVLAAVFTFLSTWLTNLAAKEKNVMMTVMIYVMPLMIFFMGFNL
                     ASGVVLYWTVSNAFQVVQLLLLNNPFKIIAERQRLANEEKERRLRERRARKKAMKRK"
     gene            complement(42216..42575)
                     /gene="rnpA"
                     /locus_tag="VC122_000497"
     CDS             complement(42216..42575)
                     /gene="rnpA"
                     /locus_tag="VC122_000497"
                     /EC_number="3.1.26.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014407282.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease P protein component"
                     /protein_id="HEP5640449.1"
                     /translation="MKKTYRVKREKDFQAIFKDGKSTANRKFVIYHLNRGQDHFRVGI
                     SVGKKIGNAVTRNAVKRKIRHVIMALGHQLKSEDFVVIARKGVESLEYQELQQNLHHV
                     LKLAQLLEKGFESEEKH"
     gene            complement(43115..43855)
                     /locus_tag="VC122_000498"
     CDS             complement(43115..43855)
                     /locus_tag="VC122_000498"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011528222.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HEP5640450.1"
                     /translation="MMNIFILEDDFIQQTRIESIVVGILKETRIPCNQLEVFSTPQKL
                     FESIQERGDHQLYFLDIEIGEYTRCGLELAAAIRQKDPNAVIVFVTTHSEFAPISFKY
                     KVSALDFIDKAGGQKQFKEQIEECIRYTYDMMSSRESKDMFLFETPQTRLKLPYKDIL
                     YFATATTPHKVCLWTQTERLEFYGNLSEIQAVAPKLFLCHRSYLVNLDKVVRIDKSKQ
                     LLYFENGDSCMVSRLKMKCLFERWEALR"
     gene            complement(43859..45142)
                     /locus_tag="VC122_000499"
     CDS             complement(43859..45142)
                     /locus_tag="VC122_000499"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002986102.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sensor histidine kinase"
                     /protein_id="HEP5640451.1"
                     /translation="MMVIYSMALFYATIFINSWVIFAKVSAIKLSWKRVSVIGISFVI
                     ANMIFDKVILIDQLFFIIVSLLSAPKKKLFEHMFNGFFTILIVELLFRVIGSFFLPAV
                     LGFSIGQINNNLKLLELCYLFVLPIFYLFSYIFSIDLSLIRFISEDKMKKWVFWMNTA
                     MFSYYFFAHFLVTVQSGFLALYFQYRSILVFIYLAIFIWVIVKLDRFAKDQLSQKLTQ
                     AQNERIAYLENYNQSIEQLYREIRTVKHDSENILISLKDSIDSGDIDLITRVYDTVIQ
                     QSATSMMRTNYEISSLDNIKEAVIRSIMNSKLLEAQYLGIELYIEIPDVIDHLPIKLI
                     DLIVLFTGLVDNAIETAKGSRRPFLSIAYFKQDNKQLFIIENSTKTNRVDIAKRFDAQ
                     QQNSAHFLTVLDSYPQITLSTKSDHYRLRQLLEMR"
     gene            complement(45139..>46430)
                     /locus_tag="VC122_000500"
     CDS             complement(45139..>46430)
                     /locus_tag="VC122_000500"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014407280.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="sensor histidine kinase"
                     /protein_id="HEP5640452.1"
                     /translation="KIYIFSFLSDITLPVWKQLTILALALFFNQFPYLSPLLIDPLLF
                     LVVLRQETKQLFSLKALFLAVAPSVLVDLLSRFMGTIVIPYLFLSSGIYLGHIIFDLL
                     AYLLIFPSFAIINYMIGKDYKMICQSGYSKRSHNFYQTLLIFVLVYYVDIFVILGFTD
                     PFLHFHHSLFVPTPYKLLFLMFILLLVYLLSYFNHSSKEYLKNELRREQQAYMTNLET
                     YGKHLEKLYRDVRAFQSDYLSRIERLGQAIKSESITQIQDIYAQTVHEANDYWDDKHY
                     NISKLRKINISSIKSLLSAKIISAEKSGIDLNVEVPDNIKETYIPELDLLLLMSIFCD
                     NAIEAALEAQQPHMSIAYFLLDDYQMFVVTNTTKKKVDINKIFEEGYSSKGSERGIGL
                     SNAQRILKKYPYLSLRTKSFDKEFSQTLTMPKEEVDR"
BASE COUNT        14042 a         9856 c         8433 g        14099 t
ORIGIN      
        1 atactccttt tttaatgagg tttcaattag tttaataaag attgtcattc tctcatggta
       61 gcgttttatt gcatcactac tcttgtgagg taagtcatta ccttagacaa aaatatggta
      121 taattaaaaa aataatactt gagtaaggat aatctataaa tgatcaaacg attaatttcc
      181 ctagtggtca tcgccttatt ttttgcagca agcactgtta gcggtgaaga gtattcggta
      241 actgctaagc atgcgattgc cgttgacctt gaaagtggca aagttttata cgaaaaagat
      301 actaaagaag ttgtcccagt cgcctcagtc agtaagctct tgacaaccta tctggtttac
      361 aaagaagttt ctaagggcaa gctaaattgg gatagtcctg taactatttc taactaccct
      421 tatgaactca ctacaaacta tactattagt aacgttcctc ttgataagag aaaatatacc
      481 gttaaagaac ttttaagtgc gttagttgtt aataacgcca atagccccgc tattgcttta
      541 gctgaaaaaa taggcggaac cgaacccaaa tttgttgaca aaatgaaaaa acaattaagg
      601 caatggggca tttccgatgc aaaggtcgtt aattcaactg gcttaactaa ccatttttta
      661 ggagctaata cttatcctaa tacagaacca gatgacgaaa attgtttttg cgccactgat
      721 ttagctatta ttgccaggca tctcttatta gaatttccag aagtactgaa attatctagc
      781 aaatcctcca ctatttttga tggacaaacc atttacagtt ataattacat gcttaaaggc
      841 atgccttgtt atcgagaagg cgtggatggt ctttttgttg gttattctaa aaaagccggt
      901 gcttcttttg tagctactag tgtcgaaaat caaatgaggg ttattacagt agttttaaat
      961 gctgatcaaa gccacgagga tgatttagct atatttaaaa caaccaatca attgttgcag
     1021 taccttttaa ttaattttca aaaagtccag ttaattgaaa ataataaacc agtaaaaacg
     1081 ttatctgtct tagacagtcc tgaaaaaact gtcaaacttg tagcccaaaa tagtttattt
     1141 tttatcaaac caatacatac aaagaccaaa aataccgtcc atattactaa gaaatcatcc
     1201 acaatgatcg cacctctatc aaagggacaa gtcttaggta gagcaaccct tcaagataaa
     1261 catcttattg gacagggtta tctggatact cctccttcta tcaatcttat ccttcaaaaa
     1321 aacatttcta aaagtttctt tttaaaggtc tggtggaacc gttttgtgag gtatgtcaat
     1381 acctctttat agaataacac aatgcttttt gtatgacatt gactgacaga gtacttctgc
     1441 agtctttttc atttttattt aagcaaacta ggccaatcct aacaattttt caaaaaaact
     1501 tcacattgta ttagcaaagt gatatactga aaatgcatta catttaaagg agtcggaaat
     1561 tgaccaaaag aattatacta actattttta cctttatttg cttttcggtg atgcctcttg
     1621 tccaagccga agatgtcatg gatattacaa ggcaagcagg ctacacagtt agtgaggtta
     1681 atcgaccaaa atcttctatt gtcgttgatg caaatagttc agatattctt tggcaagata
     1741 atattgatat tcctagggat cctgctagta tgtctaaaat gtttacgctt tacattttat
     1801 ttgaagagct cgctaaggga aaaattacga tggacactac tattacggca actccaaccg
     1861 atcaagctat cgctaacatt tatgagatta gcaataataa tatcgtcgct ggagtagctt
     1921 atcctattcg tgatcttatt actatgacag ctgtgccttc atcaaatgca gcaactgtta
     1981 tgattgccaa ttatttatca aacaatgatg cttctgcgtt tatcgatcgt attaatgcca
     2041 cagccaaaca attaggcatg actaacactc atttttcaaa cgctagtggt gcagcagcac
     2101 aagcttttca aggttattat aatcctacta aatatgattt atctgcttca aatattacga
     2161 ccgctcgaga tttgtccaag ttactctatg ccttcttaaa aaaataccct gaaattatct
     2221 cctttacaaa taaatctgtt gtgcacacta tggtaggaac gccatatgaa gaagaatttc
     2281 acacctataa ccactctctc ccagacaatc aatttggtat gaaaggagtt gatggattaa
     2341 aaacaggttc tagtcccagt gctgctttta acgccatgat aactgccaaa agaggcaaaa
     2401 ctcgactcat tactattgtc atgggggtcg gagattggtc agatcaaaac ggagagtttt
     2461 atagacatcc tttcgttaat gccttgactg agaaagggtt taaagatagt aagaccttat
     2521 ccaaaaaagc acgccaaaaa ctcgaaaaat tagtgccaca aaccaaaaaa gaaacatcac
     2581 ctaaacagca gcacttcaaa gccactaaaa agcaatcgta cttggaaaga gtcgaagatt
     2641 ttatgaatca caaccacact ttcttgctta tttgcttagc tatctttata attactatcc
     2701 ttttactaag cctcgttgtc tttgcaatgg gacgccaata atgaattatc aaaaacctct
     2761 gtcaaagcac agaggttttt gatatgttat cttacgtgtt caaggcaact aaaaactaac
     2821 cacttggggt tagtactaca actcataaaa tcctatctca aagatctttc agatggataa
     2881 gagaaaatgc aattaaccga cagaaccttc catctcatag gaaatcaatc gattgagttc
     2941 gaccgcatat tccattggca attctttggt aaaaggctcc acaaatccca tgacaatcat
     3001 ctctgtggct tcgctttctg ataagccacg gctcatgaga tagtagagtt gttcttcaga
     3061 aatcttagac actttagctt catgttctaa cgccacctgt gaattatgaa tttcgttaaa
     3121 tgggatcgta tctgatttag aaatatcgtc catcaaaata gtatcacatt caatatggga
     3181 aacggatttt ttagattgct tgttaaaggt cacttggcca cgataatcta ctttaccacc
     3241 agacttggca attgattttg acacaattga tgatgatgta tggggagcat tgtgaatcat
     3301 tttagcccct gtatcttggt gttggcctgc gttagcaaag gcaatagaca gcatggtgcc
     3361 acgcgcacct ggcccatcaa ggtaaacaga agggtacttc atggtggttt tagctcctag
     3421 attgccatca atccattcca ctgttgcatc cgtaagggcg cgtgcacgtt ttgttactag
     3481 attatacaca ttgtctgacc agttttgaat ggtggtataa cgcatgtatg caccgtcaag
     3541 cgcaaagatt tcaacgatag cagcatgtaa gctgttactt gaataagtgg gagctgtaca
     3601 tccttcaaca taatgaacac ttgctccttc atcaacaata atcaatgtcc gctcaaattg
     3661 accagtattt tcattgttaa tgcggaaata agtttgcaaa gggatatcta ccttgacccc
     3721 tttaggaaca taaataaagg taccaccaga ccaaactgct gaattgaggg ctgctaattt
     3781 gttgtctgtt ggcggaacca gtttggcaaa gtattgtttg aacaaatcag gatattcttt
     3841 gagggcggaa tcggtatctg taaagataat ccctagcttt tcaaattcac ccttcatgtt
     3901 atggtaaacc acttctgact catactgagc tgatgcccca gcaagataag cacgttctgc
     3961 ttctggaatt ccaatacgat caaaggtttc ttttattttt tctggaacat catcccaaga
     4021 acgagctggc ttatcagatg ctttttgata gtaaatgata tcatcaaagt taatatctga
     4081 taagtctgct ccccaggttt gcatcggcat tttattaaag gtttccaagg attttaaacg
     4141 aaactccaac atccactcag gttcattttt ggcagctgat agttcgcgaa ccactgcctc
     4201 actcaatcct tttcctgtag aataaatggg ctctacatca tcgtgaaatc caaattggta
     4261 gtcccctaag tcaattggct ttggctctac tttctcattt atatcagaca tttttccctt
     4321 cctttacatt ttggtccgtg aggtgctgag cattggcact tcgtttaatg gcttccttga
     4381 gagcattcca agctagggta gagcatttga tacgctgtgg aaattttgca actcctgcca
     4441 acaattctgc ttcacctagc tctttttgag caggattttc ctgtccttgt accatctctg
     4501 aaaaaatatc agctaacgcg agtgcttctt ctttactttt accaataaca gcatctgtca
     4561 tcatgcttga tgaagctgtg gaaatggtac agccgttgcc tgcaaaagca atatcttcaa
     4621 ttttatcttc gtcaaactta acggtcaaag aaatcacatc accacaagtc ggattattca
     4681 gttgaacagc ctctacgcca tctagttgcc catgatgatg tggacgtttc gaatggtccg
     4741 ctaccacagc catgtataga tggttcagtt tagagagtgc cattgaaaaa ctcctttgct
     4801 tttagaattg cttcgactag acggtcacaa tctgccttgg tgttatagat ataaaaactt
     4861 gctctaacag ttgctggtac acctaaataa ctaagaagag gttgggcgca gtggtgccct
     4921 gctcttactg caacaccttc atagtccaag gctgttgcca agtcatgagg atgcaaatca
     4981 tccagattaa aagaaatcag accagatctt gcactaggct ggcttggtcc atatatggta
     5041 agcccctcaa tagcttctaa tttcggcaag acataggcta ttagttctgc ttcatgcgca
     5101 tgtatatcag ccatgcctag tctctgaagg taagaaatgg ctgcgcttag cccaatagca
     5161 ccagctatgt gaggtgttcc tgcttcaaac ttccagggca attctttcca agtggcctct
     5221 tgttcgtaaa caaaatcaat catttctccg ccaaattcaa gaggctccac ttgattcaaa
     5281 agctcttctt tgccgtaaag aacacccaaa cctgttggcc ccaacatctt atgagctgaa
     5341 aaagcaaaga aatcacaatc caagtcttga acatcaatag ccaaatgtgg aaccgactgg
     5401 gcaccgtcaa caacaaggta ggctccttta gcatgtgcca gcttggcaat ttctttaatg
     5461 ggattgatgc aaccaagaac attggagaca tgtactaggc taacaaaacg tgtttttgtc
     5521 gtcaatttgt ttgccaaatc gtccatgtca agttggccat cttttaaata aacatagact
     5581 aaccttgctc ctgttttttg acaggcttgt tgccaaggaa tgatattggc atgatgctcc
     5641 ataatcgaaa tcaacacctc atcttctggc gtcaagacct gctctgcaaa ccgagcaacc
     5701 caattcaaac tggttgttgt tcctctggta aagagcactt ccttacttga tttagcatga
     5761 ataaagtcag caacctgctg gcgactggcc tcatatttgt gcgttgcacg ttcagccaag
     5821 gtatgaactc ctcggtggac attagcatta tcttcttgat aataggattg caaagcctca
     5881 agaacaagcg ccggtttttg tgtggtggcg gcattatcca aataaacaag gggttcatca
     5941 ttgacttgtt gatttaagat ttgaaagtct tgtttgatgt cttttgcgtc tagcaatact
     6001 aacctctttc tttttggcag taggtcttaa cgattaagca atttctcatc taaaacctta
     6061 ataatctctt ggtggactga tggaatagga atttcagcga taactgctcc taggaatcct
     6121 ctaataacca atcgttctgc tgtttcttga tccagtcctc gactcatcaa gtaatacata
     6181 tcttcagggt caacctgacc gatagaagct gcatgacctg ctgttacttc attttcatca
     6241 attaagagga ttggattggc atcggctctt gcttggtcag aaagcatcaa aacacggctt
     6301 tcttgttgag catcagctcc cttagcgcct tttagaatat gaccaatccc gttaaacgtt
     6361 aaggtgccac gttccaaaat cacaccatgc tgtaaaatat gaccgaccgt acgttgacca
     6421 tagttggtca cgcgcgtgtc aataccttgt acctgacgac cacttgaggc tgcaacaact
     6481 ttcaaatcag cttgtgagcc ctgaccaatc aaatcactgt caaaatcagc aatgacattg
     6541 ccttcattca tcacagctaa ggcccaatca atgttggcat ccttctctaa acgtcctcga
     6601 cggctaatat aggttgtcac tgaaggacct aagcggtcga tagccgagaa tttaatctgg
     6661 ctgcctgctt gggcaatcac ttctacactg atattagcgc tggtcttttg agtggcattg
     6721 ccaatagatt caaaacgctc taaataggtg aacttacttt cttttcctgc aatcactaga
     6781 acatgcttgt taaaaggaac gtcactgtca ctatcttgta agaaaatagc ttcaatagga
     6841 gttgtgattt ccaagtgatc aggaacgtag agcacggctg cgctattaaa ataagcagtg
     6901 tggtaggcag ctagtttgtc ttcatcaaaa gctaatgcct gaccaaaatg agcttcaatt
     6961 acttctggga tttcctcaag cgccgtataa aaatcactga aaacaactcc cttgtcaatt
     7021 aacgccattg gtaactgttc taagactgtt tgcgtgccta cctgaacaag ctttgggtta
     7081 tctccaatag ctataaaatc tggaacacta gctagacttt cattttctgt taaggtacca
     7141 tctcctagat tccaacggtg aaatttaacc ctttcgatgg ttggtaactc taattctggt
     7201 atggcttcta atgccgctaa acgccgttct tgcagccaag caggctcagc gtgggcttgc
     7261 gaaaaagcta ctagtttttc ttttgtcatc tgtcaatcct ttgtctctgg tttttaaatt
     7321 ggctgggtat atagaacatc acctagcttt ttgtagaaag ctaaagtaat tgtctccaaa
     7381 aacttaactt tcttccttat actcgattcc cagatcctgt gcaatacctg cgtagccttc
     7441 tttttcaaga cgagtagcca aggcagcatc accagataag acaatacgac catccatcat
     7501 aacatgaact aggtctgggg taatgtaatt caacaaacgt tgatagtggg tgataatcat
     7561 agctccaaag tctttcccac gcatctcatt aaccccttta gagactactt ttagggcatc
     7621 aatatcaaga ccagaatcaa tttcatctaa caaagcaaat ttaggctcta acatgagcaa
     7681 ttgaagaatt tcattgcgtt ttttctcccc accagaaaaa ccttcattga ggtaacgctc
     7741 tgccatctct tctttcatac ctaacaaagc cattttctca tcaagtttgg tgataaaatc
     7801 tcgcacggaa atcttatcct tatctgcctt accggcattc attgctgcac gcataaattc
     7861 agcgttggta atccctggaa tttctgaagg gtattgcatg gcgagaaaaa gtcccagtct
     7921 cgcacgctca tcaacttcta gatcgagaat attgaccccg tcaagcaaaa tttgcccttg
     7981 ggtcacttcg tagttaggat ttcccataat agctgctgaa agggtagact ttcctgtccc
     8041 atttggcccc ataatggcag ctacttcccc agttttaaga gttaggttaa cccctttcaa
     8101 aatctcttta ccttctattg aaacatgtaa attattaatc tcaagaattg acatgataca
     8161 ccccttattt tctctgtcat ctataccatc tcaagaaaag acagtgtcat ttgcactgct
     8221 ttttttataa tggtcactgc atctattata gcaaaaaaag ccttactcgg cttttttagt
     8281 gtttcaaatc cttcttttct ttccatttca gaagcattgc ttgacgataa tcactattac
     8341 caataaactt taacaagtta aacaaaggcg ttcgcttttc accccagatc tccaatcctt
     8401 caataaaaac ttctaaacca aataaaagtc caagcatcaa gaggacgcca ccaattcgac
     8461 tagaaacatt taaaagtaaa gaaataaggg aaaaaagcat ggtaatacca taaactacta
     8521 agacagctcc tcgatgagta aagcccattg ataagagtcg atgatgcagg tgcatcttat
     8581 ccggctcgta gaatttttga cctgataaac tacgtcgaat aatagccacg atggtatcca
     8641 taatgggtac accaagaata ataacaggtg tcaccacagc cacagccgtt gaatttttca
     8701 atccttgaag agacaaaacc cctatcataa agccaataaa gagggctcct gtgtcaccca
     8761 aataaatcat agctggatgg taattataag gaaaaaatcc tgcaatagca gaaatcaaga
     8821 ccaaaatggt taacgtcaaa aagaaatcct tttgaggtaa aaagaaataa gatacaatag
     8881 ccatggtcac taaactaata atagagaccc cactaaccaa accatccaaa ccgtcaatca
     8941 agttaatagc attagtgatg gaaacaatcc acaggactgt taagaataag gttaagaaag
     9001 gaccaaaaac taacaacggc cctccaaaag gaattttgaa gctatcaaat ttaaagtcgg
     9061 taaaagccca gacaatcact gcaccaatta gaataccaaa catctttaat ttaggactta
     9121 actcatacaa atcatctaaa aaaccagtaa gggtaattac tgtagcacca atcacgacag
     9181 gtaaaatata actgaagtag gtctgaccaa aaaacatggc gcctttagaa gcaatcggaa
     9241 taagtcccaa acttgctact aaaaaagaca taaaaatagc taatccacca ctggttggca
     9301 tcggaacctt attgacccgt ctggcattgg gattgtctac tgcacccaca cgaaaggcta
     9361 aaaagcgaac aagaggggtt aagaataagg acattaacaa agcccctatc agaacaagga
     9421 cataatctat tgtgaatgaa aacatactat tttatcccaa ctcaatcttt tgtaattgct
     9481 caacagcacc atcaagcatc aattgtaagc catgctcttg taaataagct cttgtttttg
     9541 atccagggtt tgcatgctca atcaatctcg catacatgac attcgcaaaa taagatggtt
     9601 gttgctgaac atctaataaa atagtcatat ggtagcagtt gctcccctta tataactctg
     9661 aagcttctat cgaaaaatca atcgttttag caaaagccac tgcctctgta atcgttgaaa
     9721 aatccaatac ataatggaca tagtctaaag gttctgactc atgattagtt gatggatcag
     9781 ccgcctcaga ctgattagct agagtagctt caacaacatc ttccattatc tcttcaattt
     9841 tttccaattt ttcatgagct ttgacatctc ccttttcacg catggattgc tccaagctct
     9901 tgaaaaaatc ttccggagtc atttgggaca tatcaccaaa ctctgctaaa tcttcaagat
     9961 taatatcttt atttatctca gatttggtaa caaaaacatc tagacggtcc ttgcgtggag
    10021 tcactcgaaa actaagcata ccactatctt taaaattatc tggaagatcg agttcgtcca
    10081 tcacagaata gaaaaactct tctgtttttt cttgaggaat caagaaatct ttcaactcca
    10141 ttcctctttc ttctaaatcg tccatactaa ttgttatttt tagcgttgtc tcgctaattt
    10201 gtttcatttc cattctcaat acctcgcacg cgtagtcatt ttattatact agaaatgctg
    10261 taatttcgca atttatgact aactcctctt ctccaaaagg aaatttaaaa agcgacagtc
    10321 cccaaggtct gtcgcaacat cctaaacgat atgtaagttt aaaaaacaaa tttaaagaag
    10381 ctataaataa gtaaaaggct tcctaacaca atacggtatt tcccaaaaat ggtgaaatcg
    10441 tgtttcttaa cataatctgt caagaaccta atcgccaata aactaaccac aaaagcggtc
    10501 aaactagcca ctaaaagaat aaggacttgt gcaaaatcaa ggtgatggcc atcaaggaaa
    10561 aatttcacag cctttaatcc actatatcca aacatggtag gaatggccaa aaagaaggtg
    10621 aaatcagcgg ccactgttcg actagttcct aaaataatag ctcctaagat ggtagcccct
    10681 gatcgactag taccaggcac aatacttaag acttgaaaac aaccaatgaa gaaagctgtt
    10741 ttgtaagaca ttctagctaa ttcagtgacg gcaggttctt gttgagcatt tcgtttttca
    10801 atccagataa aggcaatccc gtagacgatt aaagcgatgg caatcggaac cataaaataa
    10861 aaatgtgctt caaaccaatt atcaagagga accgctatta aaatagaagg aatacacgca
    10921 atcaccactt ttagccataa ttgccaggta agctgtactt ctcttgctgt tttcccaggt
    10981 tgaaagggat tgagacgttc aaaataaatc aacataacag cgataatcgc ccctaattga
    11041 ataacgatat taaacatttc tataaaagcc ttatcctgat ttagtcggat aaattcctgc
    11101 accaaaatga ggtgaccagt gcttgaaact ggtaaccatt ctgtgattcc ctcaatgatg
    11161 ccaaagaaaa tggcttttag caattcgata attaacatgt tttctcctta ttaaccactc
    11221 ttattgtgat tttctaaact gtgattaatg ccaagcagct tttttaaaag gcaccgccgc
    11281 cgcctcctcc gccaccgcca gagaagccgc caccaccact aatcccgcct ccggaagaga
    11341 ctgagaaatt tgaggaagtt gtagctgaag acaagctaga caaaaaggtt ggtgtcgtag
    11401 catacattac cattgataat tcacccggtc gaacagcttg gtaaacctca ggtaaggcaa
    11461 tacgatggac tttcaaatag cgttccactt tcttagcgta accaaataag gtggcataaa
    11521 ccaaaacacg gttccaaaca accaaacctt caatagcaac atcctcaaat ttgtcaatgt
    11581 ctcgtatcat gtttttaaag ctctgccatt gatgtaaccg aacacccccc tcaggtgttt
    11641 caatacccaa tttcttgtat tgatctaact tcaggctaat gtaaaagcca aggcacatcg
    11701 ttagcaccat taaagcaagg taaatgaggg ctaacccact ttctttaata gccaagtaaa
    11761 tcaataaacc actatttaga acaagcaaca aacaacctaa tccctggaca ctattgcttt
    11821 ttcgtttctc tgccggggtc atttgacgat aagtatctgg taagtttaac tttttgatag
    11881 tttctaggac attgtttgta atagctgccg acgctttttt catcgcctta atcacctgct
    11941 cagacgattg acggacttct tgctcgagct ttttaccttt gtacgttttc tttaattgtt
    12001 taatggtatc agcatcataa tggtattgac taaacaattg atccaccggc aatgtcacct
    12061 tgtttccaaa agccatatct aagaaggcag cttcctcatc tgtcacacga tctaactgac
    12121 tgatttcaag ataagctttc ccttcttctt tcgttaataa aagcaccttt tggtcgatca
    12181 gatccaacaa ggtagcttga acaagggcct caaaggtcac tcctttaggg atcaataatt
    12241 tctggctttc tgatgctgta ggggataaat aggcaaaact ctgtccataa atcgcttgtg
    12301 tcaacacaag aggcgaaaga tcttcaggga cttcgtaact gtgatctgta tggttggcca
    12361 aatgataacg attgaattgt ttctttaatc ttataaactg tattaactgc cacagaagaa
    12421 aacctacctc taccagagga atgactttgc caaatagcag ctgtaacaag gcccttcgtc
    12481 tggaaatctt agtttccaac gctacaattt tattcttttt actcgttgaa acatcaactg
    12541 gttcttctcc caataatgct tttttatccc aataagcatg caattccaat tgccctgaga
    12601 catttttggc attaatctga tagcgactgt taccttcttt taacacttga ggtttctttt
    12661 ggtaatatcc tctgtgagcc catagattag aatcctgaat atcagttggg gtagtgacgg
    12721 tcaaactcac cttttctaat gttttgtccc aatcactaat cggtgtccaa ttcagctctg
    12781 ccacgtcttg ataagctgtt aaaaggtggt gcagttgcca gataacctta acgtcaacct
    12841 tatcaccagc ttggccagca ttataaagtt ttaaacgata gccatctcca aggtcactaa
    12901 actcttgact aacaggtacc tgttgaccat tctgatatac ctcaacttta ggcttttggt
    12961 caatagcaaa gcccgctggc aaatgacctg cccggcccag cgaaatatac tggccgttat
    13021 aagaagtgtc aaattgatag gttacttttt gctcaaaccg agcagtattt tcttttgata
    13081 acagtaactg accttcatag ttggtgatgc tatagtctac atcagctgct tttattcgta
    13141 tccctaaaag actgaagcag agcacaagcg tcatcaaaat ttttttcatg gcaacatcct
    13201 ctttctgccc tcattttacc atattttaag gagatgacaa aacctaaacc aagtccttca
    13261 tcttattttt aatcattatt ttgaatattt ttccatattt caacctattc actgataata
    13321 tattgaaata ggatgcaaat tgctgtaaaa taggtctgaa tactattttt gaaacaggag
    13381 acctatatga aaaagctaat ccttagctgt ttggtcgcct tggcccttct gtttggagga
    13441 atgagccggg ctcaagcaaa ccaatattta agagtcggaa tggaagcagc ctatgctcct
    13501 tttaactgga ctcaagatga cgcttcaaac ggggctgtcc ctattgaggg aactagccaa
    13561 tacgccaacg gttacgatgt ccaagtcgct aaaaaagtcg ctaaagctat gaacaaagaa
    13621 ctgttagtcg ttaagacctc ttggaccggt ttaattccag cattaacttc tggaaaaatc
    13681 gatatgatcg ctgctggtat gagtcctacc aaagagcgta gaaacgaaat tagcttctca
    13741 aacagctact acactagcca acctgttcta gtcgtaactg ctaatggcaa atatgctgat
    13801 gcaacaagcc tcaaggattt ttctggagct aaagtaactg cccagcaagg cgtttggcat
    13861 gtcaatctct taactcaact aaaaggtgct aagttacaaa caccaatggg agatttctct
    13921 caaatgcgac aagcccttac ttcgggtgtt atcgatgcct atatttctga acggcctgaa
    13981 gccatgactg ctgaagctgc tgatagccgt ttgaaaatga tcactcttaa aaaagggttt
    14041 gctgttgctg aatcagatgc tgctatcgct gtcggaatga aaaaaaatga cgatcgtatg
    14101 gcaactgtca accaagtgct tgaaggattt tctcaaacgg atcgtatggc cctgatggat
    14161 gatatggtta ccaaacaacc cgtggaaaag aaagccgaag atgctaaagc atcatttcta
    14221 ggccaaatgt gggctatttt aaaaggtaac tggaagcaat tcttacgtgg aactggaatg
    14281 acccttctga tttccatggt cggaaccatt acaggtctct ttattggatt attaatcggt
    14341 attttccgta cagctcctaa agctaagcat aaagtagctg ccttgggaca aaaactcttt
    14401 ggttggttac tcactattta catcgaaatc ttccgtggga cacctatgat tgttcaatct
    14461 atggttattt actacggaac agcgcaagcc tttggtattt cgatcgaccg taccctagcg
    14521 gctattttta tcgtatctat caatacgggt gcctatatga gtgaaattgt tcgcggtggt
    14581 attttcgctg tcgacaaagg tcaattcgaa gcagcaactg ctcttggctt tactcacgga
    14641 caaaccatgc gtaaaatcgt gctaccacaa gttgttcgca acattttacc agcaacaggt
    14701 aatgagtttg tcatcaatat caaagatact tctgttttga atgttatctc tgttgtggaa
    14761 ctttacttct caggtaatac cgtagccaca caaacctacc aatatttcca aacctttacg
    14821 attatcgcca ttatctactt tgttcttacc ttcacggtga cacgtattct tcgtcatatt
    14881 gaacgccgtt tcgatgccga tacttacacc actggagcaa accaaatgca gattgcggag
    14941 gtctcaaatg tctaactcaa ttattgaaat caaaaacctc aaaaaatctt acggcagcaa
    15001 tgaagtactc aaagatatct ccctttctgt taacaaggga gaagtgattt ccattatcgg
    15061 ttcttctggt tctggaaaat caaccctcct gcgctcgatc aatcttttgg aagagccaag
    15121 tgccggtcaa atcctctttc atggtgaaga tgtccttgct gaacactaca acttgactca
    15181 ctaccgtgaa aaactaggca tggttttcca atcctttaac ttgtttgaaa acctaaatgt
    15241 attggaaaat gctatcgtcg ctcaaaccac cgtcctcaaa agagaccgtg cacaagccga
    15301 acaaatcgct aaagaaaacc tcaatgcggt tggtatgaca gaacaatact ggcaagccaa
    15361 accaaaacaa ttatcagggg ggcaaaaaca acgggttgcc attgccagag ccttatctgt
    15421 caatccagaa gccatgctct ttgatgaacc aacatcagct cttgatcctg aaatggttgg
    15481 tgaggtcctt aaaaccatgc aagacttggc aaaatcaggg ttgaccatga ttattgttac
    15541 ccatgaaatg gaatttgctc gtgatgtttc tgaccatatc atcttcatgg ataagggatt
    15601 gattactgaa gaaggaagtc ctcaacaaat tttcgagaat cctacacaag atcgtaccaa
    15661 agaattttta caacgcttct taaaataaga taaaggatcc aactatttct atgccagttg
    15721 ggtctttttt caattcaaaa agaatttgtc ataaaatact ttaccagtta actattttat
    15781 gctatactta tttttgttaa cgttaataat ttatatttag gaggataata tggcagaaaa
    15841 aactgactta tcgtcagctt acagaagact gaaaagtccc aatattaaaa ccagaaaacg
    15901 tgctctgaag attattcacg agtacaaacg ttacggtaaa aaataagatt ttacttttta
    15961 tctctccaaa tttaagtcag acaaaaaaag gctaagacac cccttccatc ttagcctttt
    16021 tgtatacagt ttattaaggg aactatttta aaatatcttt tatgctagag tctagaatag
    16081 ttcaaaacgt taaaatcata catttaaggt agtgtttaaa gtatacgaaa aagaactcag
    16141 cataaaccaa gctctctttc gttataacta attatttagc aatttttgcg aagtactcaa
    16201 gagtacgtac aagttgagca gtgtaagaca tttcgttgtc ataccatgat acaactttaa
    16261 ccaattgtga tccgtcaact tccattactt tagtttgagt tgcgtcaaac aatgaaccgt
    16321 atgatacgcc tacgatatct gaagaaacga ttggatcttc agtgtaaccg aagctatcgt
    16381 ttgaagcagc tttcatagca gcgttgattt cgtcaacaga aacgtttttg tcaagagtta
    16441 caaccaactc agttactgat ccagttggaa caggaacacg ttgtgcagca ccgtcaagtt
    16501 taccgttaag ttctgggata acaagaccga tagctttagc agcaccagtt gagttaggaa
    16561 cgatatttgc agcaccagcg cgtgcacgac gaaggtcacc accacggtgt ggtccgtcaa
    16621 ggatcatttg gtcaccagtg taagcgtgga ttgtagtcat aagacctttt tgaataccga
    16681 atgcatcgtg aagagcttta gccataggag ctaaacagtt tgtagtacat gaagcacctg
    16741 agataactgt ttcagtaccg tcaagaatgt cgtggttagt gttgaaaaca actgttttaa
    16801 catcgtttcc accaggagct gtgataacaa cttttttagc accgttagca tgtaagtgtt
    16861 tttcagctgc ttctttttta gcaaagaaac cagttgcttc aagaacgatt tcaaccccat
    16921 cagttgccca gtcgatgttt tctggatcac gttcagcaga aactttgatg aagtttccgt
    16981 ttacttcaaa tccaccttct ttaacttcaa ctgttccatc aaaacgacct tgagttgtat
    17041 cgtatttcaa caagtgtgca agcatatttg gatctgtaag gtcattgata cgagttactt
    17101 caacaccttc gatgttttga atacggcgga atgcaagacg tccgatacga ccgaaaccat
    17161 taataccaac tttaactacc attagtgatt tcctccttat gaaaatcaaa agaatattat
    17221 ttagagggaa tgctccctac ctattgtgaa aagattaact tacagtcatc tgcaacaacc
    17281 tttcaacaga actattatat aactatttca gaaaaaatgc aaataaatta gccattttca
    17341 taaagggcag cttctttctt ctagccttca aaacccacca aatataggaa agggctttct
    17401 tttttctatg ttctattctg taatttatct ttttattggg aaagatttcc aaaacttcaa
    17461 actgtactat aacgttctgt tacactaaaa aagaggttag gatagttatc ctcaacctct
    17521 tagtggtggt tattcacctt tgtttttctt aatgatttct tcttgaactg acttaggaac
    17581 atcttcatag tggtcaaata ccatcatgaa tgtaccacgt ccttgagtag cagaacgaag
    17641 aactgttgca taaccgaaca tttcagcaag tggaacataa gcacgaacga tttggctgtt
    17701 accatgtgct tccataccgt caacgcgtcc acgacgagct gtcacgtgtc ccataacgtc
    17761 tccaagattg tcttctggag ctgtaatcgt tacaagcatc atcggctcaa ggatagctgg
    17821 ttgtgcagat ttagctgctt ctttaagtgc aagtgatgca gcgatcttga aggcagtttc
    17881 agatgagtcg acatcgtggt atgaaccatc gtaaagctta gctttaacat caaccattgg
    17941 gtaacctgca agaacaccgt ttgccataga ttcgatcaac cctttttcaa cagctgggat
    18001 aaattcacga ggaactacac caccaacgat agcattttcg aattcgaaac ctttaccttc
    18061 ttcatttggt gtaaattcaa tccaaacatc accgaattga cctttaccac cagattggcg
    18121 tttgaagaat ccacgagctt gtgtagaagc acggaatgtt tcacggtaag atacttgagg
    18181 agcaccaacg ttagcttcaa ctttgaactc acgtttcata cggtcaacaa ggacatcaag
    18241 gtgaagttca cccataccag cgataactgt ttcaccagtt tcaacgtttg tttcaacgcg
    18301 gaatgttgga tcttcttcag caagtttttg aagggcaaca cccatcttgt cttggtctgc
    18361 tttagatttt ggctcaacca tcaactggat aactggttct ggaacttcga ttgactcaag
    18421 aataactttt gctttttcat cagtcaatga gtcaccagtt gttgtgtctt tcaaaccaac
    18481 agcggcagcg atgtcaccag cgtaaactgt ttcaatttct tgacggctgt ttgcgtgcat
    18541 ttgaaggata cgtccgatac gctcacgttt accttttgat gtgttcataa cgtaagaacc
    18601 gctattaaga acacctgagt aaacacggaa gaatgtcaaa cgacctacga atgggtcagt
    18661 catgatcttg aaggcaagag ctgcaaatgg ctcttcatca gatgctggac gttcttcttc
    18721 agcatctgta tccgggttaa cacctttgat tgcagggatg tcaagtgggc ttggaaggta
    18781 tgcgataacc gcatcaagca tcaattgaac acctttgttt ttgaaggcag aaccacaaag
    18841 tactgggaag aattcaacgt tgattgttgc tttacggata ccagcaatca attcgtcgtt
    18901 agtgatttct tcaccttcaa ggtatttcat catcaagtct tcatcagttt cagcaactgc
    18961 ttcgattaat ttttcacggt attcttgagc ttgttctaag tattcttctg gaatatcttc
    19021 ttcaaggata tctgtaccaa ggtcgttggt atagatttct gctttcatct tgatcaagtc
    19081 aatgatacca cggaaatcat cttctgcacc gattggcaat tggattgggt gtgcatttgc
    19141 ttgaagacgg tcatgaaggg tttgtactga gtaaaggaag tcagcaccga ttttatccat
    19201 tttgttggcg aaaacgatac gtggaacacc gtattcagtt gcttgacgcc aaactgtttc
    19261 agtttgaggc tcaacacctg attgtgagtc aagaacggtt acagcaccat caagaacacg
    19321 gagagaacgt tgtacttcga tggtgaagtc cacgtgtcct ggtgtgtcga tgatgttaac
    19381 gcggtgacca tcccattgag ctgttgtagc ggcagaagta atagtgatac cacgttcttg
    19441 ctcttgctcc atccagtcca tttgtgatgc gccttcgtgt gtttcaccga ttttatgaat
    19501 tttaccagtg tagtaaagga tacgctctgt tgtagttgtt ttaccagcat cgacgtgagc
    19561 catgataccg atattacgag tttttgcaag tgaaaattcg cgagccatgt gtttctccta
    19621 tttttttagt ttactctttc attatagcac tatttagcaa aaacggatag gcaggaccta
    19681 cccgttatct aaatatttgt tggtaaagat aatacctatt gcctttataa ggcttggtat
    19741 cttattcatt gaactcaggc taaacgctgt gtgaaaaaga taaactttcc tagagacaaa
    19801 ggtttcttcg tcaaatttcc tattttcact ttgcgttcta tacgccttcg tatcttatta
    19861 attgagttcg agctacaagc cactgacaaa aagataatag ttcctagaaa ctgaagtttc
    19921 tgcgtcaatt tccctatttt gtctagcatt tgtttgacgc taaaatatct tatattacca
    19981 acggaagtga gcaaaggcac ggttagcttc agccattttg tgagtgtctt cacgtttctt
    20041 aactgatgca cctgtgttgt ttgcagcatc catgatttct ttagcaagac gatctttcat
    20101 agtgtgttca ccacgagcac gtgatgcgtt taccaaccaa cgaagtccaa gtgttgtacg
    20161 acgttctgga cgaacttcaa ctgggacttg gtagttagaa ccaccgacac ggcgtgcgcg
    20221 tacttcaagt acaggcatga tgttgtccat agctgtttca aatacttcaa gagcgtcatt
    20281 tcctgttgct tctttgatag cgctgaaagc atcgtaaacg atagtagcag ctgtaccacg
    20341 tttaccgtca agcataacac ggttgataag acgtgttacg atttttgagt tatataatgg
    20401 atctggtaat acttcgcgtt taggcgcttg atttttacga ctcattttct cttatccccc
    20461 ttctattatc cttttggacg tttcgcaccg tatttagaac ggccttgttt acggtcagca
    20521 acacctgcag tatcaagtgc gccacgaacg atatggtaac gtacccctgg aaggtctttt
    20581 acacgtccgc cacggataag aacaacgctg tgctcttgca agttgtgtcc gatacctggg
    20641 atgtaggcag ttacttcgat aaggttgctc aaacgtacac gagcgaattt acgaagggct
    20701 gagttaggtt ttttaggtgt cattgttcca acacgagtag caacgccacg tttttgtggg
    20761 gcagccattt tagtttgaac ttttttgtga ctgttgtaac caatgttcaa agctggtgaa
    20821 tctgattttt caattttaga tttacgtggt ttacgtacca actggttaat tgtaggcatc
    20881 tacattctcc tgtgtttttt tatttttggt tgatatagca cttggtgaca gcccatatcc
    20941 ggtgcacttt ttgcaacaat tgtcagcacg tctctgtaca cttttgagag accaaaagta
    21001 aaaagtaccg tctaatataa taacacagac ggtacttcat gtcaaataga atttttcctt
    21061 tgatgcaatt gcttatgacg aatgcatcac ttagattcct tacggaacct aaatcctgtg
    21121 aggccgacag ctgccaacat gacgactcct gctttaagag ctctttgagt actttcatca
    21181 acatcagaaa tgagattgct cgttgtagaa gatcctgagc cataagatgt cttagatgag
    21241 ggctgagtaa tcgaagatgt gattttagac acaatggcat tacttgctac catttcagag
    21301 gtttggccaa caagcgcttc tgctacaaga cttgtatttt cagcagctaa tcttgctgaa
    21361 gcttctaata gttgtttcgt ttctttaacc atttcttgaa caagcggagt agtatctatc
    21421 ttactatatg atagcggttt tacgcccgta agaggtggag ctacttgcaa atcaggcaca
    21481 gtagctatat cttcgtctaa atggcgatat cccttttcat tagctaaggt tttaagcaaa
    21541 gtcaactggt gttctgtggt agcaatagta gcttctagac tgctttgttt agcttgtaag
    21601 gctgctaaag tttcttgtgc atttaacaaa gatgaggtag ttttagctaa atcttgctta
    21661 gtattatcaa tgcgctcacg tatcacttga aggcggttag gattcaattt aaagtccctt
    21721 agatattgca aatgagcttt tttagccacc agtgctgtca ctctggctgc ggcttgctct
    21781 gctaagactt ctgtctggtg cagtgcggct ttcaacgatg ctagcttagc taatgattga
    21841 tcacgagtag cttcgagttg tgcttgtttt gctttagctg ctagtaattc tgttctcaaa
    21901 gaacctttag tatcatttaa ttgtctcact tggaggttta agctgtctga ctgcttaagt
    21961 gtgcttgcta atttaccttg aagttgactt actttagctt gggctgccat aatgtcagct
    22021 tcttgatgaa tagccgacaa acgattttct aatgagctta cttttccttt gatcgctgca
    22081 atagtatcag atacagtgcc tactctttgg gcataatctt ttgtacttcc aacggctttt
    22141 ataggggtct tattaaagcg ttgatggtta gcaatgttag actctggaaa cattacaaag
    22201 tgttcattca aagatcctac attgctggtt gaaaatccaa ggtaaacagg cgcattaggg
    22261 ttacgtttat ctacacgtaa aaagttaata gcatggccgt atgtatttcc gtgtaaatga
    22321 tctgtaaaga gcatatactt gatactgtca taaataccac gtttaatacc attcacagta
    22381 tgcacatcgt taaaagcacc gatattctcg tacatgttat catcatttcg aatgagccct
    22441 gacgctccgg cagagtcttc aataatagtt ttatcatgag gcccaacacc ataatgccct
    22501 gatacccctg gctgtccgta gacaaatgat ggtcttgtat taccatgagt tttcttatag
    22561 ctggtactaa gtaatcttgc aaattcttgt gatcctgctg taacagtaac tggtggtaga
    22621 cctaattgtc ttcttacact attaatcatg tgagctgcaa actgcgctag ctcattttgc
    22681 acttctggtg tcaaattatc gggatcaaca aagcgattac gatctgctgg aatatcttgg
    22741 tattgattta attgattacc tggactagct ttggcaataa tttgatctgc atgctctttg
    22801 taataattat tgtaactagc tgatccaata taaccactag cttctaattt tttaagttct
    22861 tcaagaggat agccttgcgg tactttcatg gtattattac ccacaatgct atcttgagta
    22921 gacggagctg aggatttaag acgactaagt tctgcctctt tttctgctag agcagctttc
    22981 tgagctgcca actcttcagt caattgcttt ttaactttag ctttttgatt ttctaagtca
    23041 gctttagcct tctccaattc tgagcttaat gcttttgtat tatcattagc cgtttgagct
    23101 gctttagtga tagcatcagg attgctaatc atagcattga gcttagcaat attttgttca
    23161 gacgttttga cttgttccac taaatcttga gctcgagtag tttctgctga aatgctagct
    23221 ttttgttctg acaatgcagt ctcttttgaa tgttgatctg cttgagcatt atgaagctct
    23281 gtttcagtag ctgttaactc tctttgatgt tcggctcctt gggctaatag cgtctcctca
    23341 ctacttgcaa gagtattagt gtaaatttct tgtgcagagg ttaaagcttt ttgttcatta
    23401 tcttgctgct cttttaagtg gttgatttca gcagtagttt ttgtcagagc ggtagcaagc
    23461 tctgtcagtt ctgctttttg ttgactgaga gttttttcaa ctgcatcaat agttgccttt
    23521 gcctcttgaa ttgtttctgg ttttggtaaa ctatcgtcgt gagtattact cgccttcgtt
    23581 tctcctgagg ctctatcatc tgctttgact tggtgagata cgcctacact ggcactcaat
    23641 aaagcaattg ttgaggttaa agcaattttc tgcttaactt ggtttggctt cgtttgttct
    23701 aagtccattc tctctccttt ttccatttat cgaaaatatt accaacttct acaacaacat
    23761 tgttgttttt gacatcaatc tctgaaactt tcagcaatga tttgaaggtc atttgctcac
    23821 gctctgattg agcgttttct gcaaacacag caacaccaac taaagtgctg tcaaattctg
    23881 tcaagagact gatcattcct gtaatcgtcc ccccgccctt caagaaatca tctacaatca
    23941 atacacgact attgggtttc aaactacgct ttgacagaaa catcttttca atacgatcac
    24001 tagaagcact cgcataattg acagacactg tcgatccttc tgttattttt aaatcacgtc
    24061 taacaatgac aaaaggaaca cttaaaatat tagccactgc atttgctaaa ggaactcctt
    24121 tggttgcaac tgtcatcaca gcgtcaatct tttctccttt aaaggcattg gcaataatac
    24181 gaccaatatt ttgtaaaatt ttaggtgtac tcagcaagtc tgacaaatag atatagcctc
    24241 caggtaagat gcgatcactc tctgataacc tttgacacag gtcttcaaca atggtacgcg
    24301 cttctgtttc tgaaatactg ggggtaaaaa ttacgcctcc acttgctcct gtaagtgtat
    24361 cgatatcacc aatgttagct tcttcaaaag ctttcttgat aatagcgata tcttctgaga
    24421 tagaagattt tgcggcctca tatttagttg caaaggtgtt taaactagtt aatttatacg
    24481 gattgttaat caaataattt gaaatgacaa ccatacgttc acttcgtctt aatttcatag
    24541 tttccccatt tttatgtact ttttgctatt ataccataaa gttaataaaa aacgaacatt
    24601 cgattaggaa aatattggtt tttttgttct tattaatatc atgcaagaat tcaatctaag
    24661 ataaactaat caacattcct actgaaagga cattctactt aataaccata acaagagcca
    24721 aaaacactta tgaaaccatc ataagtgtta agcacatcaa aaatcatcac aaaagcaaag
    24781 taactattac ttgagaaaca ctgatcaata gtttggcttg taaaaagacc gattatccat
    24841 ggcaaaaatc cgattggtca tctcgccctc gctaacacgg ctaagagctg ttgtcatcat
    24901 catcatatta gcatcaatat tgtcaatcat atgaataatt tcagcttcca tgatgcgtgg
    24961 acgtaccgga ctaccgtatt ctaattgccc gtgatggctt aaaatcacgt gcctaaggac
    25021 aatgacttct tctttggtat catcgatttg tagttctgaa attacttttg taatttcttc
    25081 gttaatgaga gaaatatgcc caatcaagtt accacgaacg gtgtattctg tattatctgg
    25141 tcctgttaat tctatgactt tagctaagtc atgtaacata atacctgcaa acagtagact
    25201 cttatttaag tcagggtaga tatctccaat gctatctgct agtctgacca tcgtagcagt
    25261 atggtaggcg aggcctgact caaaagcatg gtggtttgtt ttagctgcag gataagtata
    25321 aaattcttta tcatacttac gataaagagc ccttacaatt cgttgccacg ttgcattttc
    25381 aattttaaag agcatctgtt ccaaatagtc acgtacttct gtcacactga caggtgcctt
    25441 ttctttgaag tcctttggat cattgggctc acctgggcga acatttcgca aggtgatttg
    25501 attaacctga ggggtaccat tatacacctc gcgtcgccct ttcataaaca cgactttacc
    25561 agcagtaaat tcttcaacgt tataaggttg ggcatcccac agatttcctg aaatctcgcc
    25621 agtgtcatcc tgaaaggtaa ggctaataaa atctttacca gctcttgtct tacgaacctc
    25681 tgccgactta atgaggtaga acccttcaaa aagctggtct tttttcattt gattaatttt
    25741 cattattttc ctcttctaat aagggcatgt tcaagagaga ttttgttgtt tggtcttgat
    25801 aagtttcaac ggtattcaag gctcgaacaa tggcatttgt ccttgttgaa atgagctgat
    25861 ccagcgtatt attagctgta ttcatttgct tttgagcctt ggcaagcagg ccaccaaatt
    25921 tatcgaattc taacttgaca ttgcccaaaa ttttactgat gtcatcagca tttttttgga
    25981 tattaagggt cttgaaacca acagataagg aattgagcaa agcagacagg gtcgaaggac
    26041 ctgcaaccac aatattttct tcccgacgaa ggctatcaaa gaaagccgca tttctgacca
    26101 cttctgaata aagaccttct gttggtaaga acataactcc gaaattggtc gtctctgggg
    26161 ggttcaagta ctttttatga atgtctttgg caaagcgttt gatagctgcc agaagtgctt
    26221 ttcggctagc ctcgatggcc agtttatcac caacttcgta agcatcttct aatcggtaat
    26281 aatcttcaag agggaatttt gagtcaatcg gtaggtaaat ataaccgcct tgaccatttc
    26341 ctgggagctt aatcgcatat tctacgcgtt cactagaacc actaaccgtt acaaattctc
    26401 tttcgtactg gcttgatgtc atgatatcct caatgatttg gcctagttga agttcgccta
    26461 aaatgcctcg tgttttggta ttggacaaaa ccttatttaa agtacccaca tcttgagcca
    26521 cgctacgcat ttctcccaag cctttattga cactttctag ttgcttggat acagaatcga
    26581 aagaggcgtg cagacgattt tttaaggttt cttccaattt ttcttcaacg atctggcgca
    26641 tttcctccaa acgtttttcg ttagattctt gcatattttt gagcgattgg ttgacctgct
    26701 ggttaatttc ttctaagcgt ttgtcagacc ggtccctact atcagacaaa ctacggtgca
    26761 agacgtcacg aatatctgtt aattgttggt aaaggtctgc ttgttgtcgg ttcatcagct
    26821 gtgttagctc taacaattgt tgcttgttag ctgtatccaa ctggtaggtt atttggtcag
    26881 acaaattatc cgcgttgcct tctagggttt gggcaagctg atgttgaagg ccgttgactt
    26941 tgaacaacag ataagcccct aaacctaaga gaaccaagac caaaaggaac aagataaggt
    27001 ccatactaac tccaatcttt actttgaata ataatcaagt agccacttgg caaactaact
    27061 gtaataggtt gatggataaa ggcattactg gaatagattt tctttttgaa aaaattatct
    27121 tgcgtcaact caaatttagc accagtaatg gtcaagtcag cttctccttc tgccataaat
    27181 gccacatagg tcatcccttc ttcttgatgg ataaggtgct gacctgccgg gcggtaggta
    27241 atcatatttt gttggtcacg caaggcgatt tgtgccataa agggagcaat accagggtcg
    27301 ctaggcaaga aaatattgga taagaggtgg tccattcgac ccccaaaagc cccaaaaatg
    27361 gtcacttccg cctcaggaaa acgagcaaag acctctttaa gggctaattc cgtatccgta
    27421 tcattttttt ctggatgagc agtaataaaa agctctgcct tctcttttat atccgtaaac
    27481 gctttttggg aaaccgaatc aaaatcacca acagccatat tcaaaggaag cccattttct
    27541 aataaaaata ggctccctct atcaataccg acaaaataat caaaatcacg gctgatataa
    27601 gagagatctc ctcccgcaaa aagagcaact ttagacattc aaggccgtcc ttaaggtttg
    27661 gacttggctc actaggtcac tggccttgaa aaggtaagaa cctgccacaa agacattggc
    27721 ccctgcttca taacaagcac gaatggtctt attgtctact ccgccatcaa cctcaatgtc
    27781 aaaggacaat cctttttcat ctcgccattt ggctacggta gccacttttt ctaaacattc
    27841 tgggataaag gcctgacccc caaaacctgg gttgactgtc ataatgagga cttggtcaac
    27901 taagtctagc aaaggttcta gggctgttgc aggagttcct ggattaatca ctacccctgc
    27961 tttcatgcca gctgccttga ttttttgaag ggctccatga atatgacgag tactttctgt
    28021 gtgaatggtc atgatatcaa caccagcttg agcaaaagcc tcaacatagc gttcaggatc
    28081 aaccaccatt agatggcaat caaagaccaa tttactgtgc tttctcatac ttgccacaac
    28141 atctgcacca aaactgatgt taggaacaaa ttgaccatcc ataatatcaa tgtgaacgta
    28201 ttcggcatct gtttcttcaa tacgagctag ctcagaagca aaattagcat aatcagctgc
    28261 tagaatagat ggggcaattt ttagagttga catggagtag acctactttc ttttgataac
    28321 ttttttatat gtttcacgac ggttttcaat ttcactgaga aattgcaggt aatgctcata
    28381 acgaactggc cacaactctc ccgtttcaag cgctgccttg acagcacact tgggttcgtg
    28441 ggtatgggta caggatctaa acttacactc atggctcagc cggcgcagct ctggaaaagc
    28501 ctcgttcaaa tcttcagcat tagcaatatc atagtctaat gatgaaaaac ctggcgtatc
    28561 cgcgattttt ccgccatggg tattgtaaaa actcacagct cgggtggtgt gacgaccacg
    28621 ccctagacta tctgagattt ctcctgtttc aagagccaat tcgggggcga ttcggttgag
    28681 aagggttgat ttcccaacac ctgtttgtcc cataaagacg gtaattttat cagctagcaa
    28741 aggcaacaat tcctcaagac tggtcacaaa atcgtaaccg attgcctgat actgtcgacc
    28801 aatagcctta atctcctcgg gactatctaa taagtccatc ttagagatat aaaccactgg
    28861 atgaatggct ttgtgttcaa gcaagatgag aaagcggtct agaagattgc tgttaaattc
    28921 tggttcttta gcagacataa taaccactgc ttggtcaata ttgacgatag gaggtcttac
    28981 caaactgttt ttacgaggat gaatcgctaa aatataacct tcagagttgt cctctgctga
    29041 gaaatccacg atatctccaa cataaggtgt ctcaccgcgc ttcctaaaat taccacgcgc
    29101 tcgcgtctga tatacctgtc cttctgactc cacgtaataa aaaccagcca gagatttaat
    29161 gattttacct tgcaactgtg ctcctttgcc ctactataat aactctatta tatcagaaaa
    29221 ccgctcccaa atcagcattt gactctaagt ttcactcgcc ttaaaaaact gtcaagattc
    29281 tctagaccac tggctatcat ttaggtcact gctttttaaa aatagaatat tttatcaact
    29341 aaaaatcaga tcactgatga tttttgaggt aggaaacgat atgaatttta agaagacctt
    29401 tgctatttac gaaaaagagt ctaaccgtac cgctatagtc acgctcttgt taattgcccc
    29461 ttttttccgg ggtattaaaa aaactagctc tgcactgcga taccttaacg gccagcttca
    29521 ataagaaaaa tccccctgaa ctgaacattt ctgttttgtc tacaagggaa tttttttatt
    29581 gtcaccggga gaaagctttt ataatccaac ttcttttaaa gcatcagcta atttgccaaa
    29641 atcttgaatc gacaaggcct caccacgaat agaaggcttg atatctgcta gtgctagacc
    29701 tttttcaagt ttagtcttga tgtcttcaga cttcccaaaa tggctagtca agttattcca
    29761 cagggtcttg cgacgatgga caaaactgag acgagatact ctgaaaaaga aatcttcgtc
    29821 cttaacctca atcaaaggct ggtcgcgacg caccattttg aggatagcac tatcgacatt
    29881 tggtgcaggt acaaagacgg tgcgcggcac aatgaaggca accttggcag tcatgtaata
    29941 ctgcaccgca atggacaagg aaccataagc tttagtgttt ggctcagccg agatacgatc
    30001 tgccacttca cgctgcatca tgactacaaa ctcttggaat ggaattttgc tctcaatcaa
    30061 gtgcatgaga ataggcgttg tgatgtagta aggtagatta gccaccacct taatgggcaa
    30121 atcaggattt ttaaactgct tgatttgggt ctgcaaatca gcttttaaaa tatcttgatt
    30181 gaccacttgc acattatcaa aatcgcgcaa ggtatctgcc aaaatcggca ccaagcggtc
    30241 atcaatctca aaagccatca cttcagcagc attttctgcc aaaaattccg tcaaagcccc
    30301 aattccagga ccaatctcga taacattgac attctgatca atctcagccg tgtcaacgat
    30361 tttctgtaaa atattggtat cggtcaaaaa gttctgacca aaagatttct taaaggtaaa
    30421 tccgtgacga tcaaggacag ccttggtcac gctataatct gcaattctca tatattctct
    30481 tctttttatg atcactttct ttttggcatt tgaaccacta gcctaaactt ttttgccaag
    30541 atacgccatt cagttaatgg ggctgttata ttttattgta ctctttactc ctcatatgtc
    30601 tccatcactt cttccacttc tgctagggta atgccaaata attcgaggcg cttgagcagt
    30661 tgtttgccgt tggcatagcc gatgcggagc ttttcgccca agtattccct gcgtttacgg
    30721 ctatctgacg ccattaaaag ccctagacga atcaggtctg tttgacgaat gtcaaaataa
    30781 gactcatcat catattgctg agtgacatga gcaagtgcct tttggaggtc ctcaaaattg
    30841 gcatgttcaa cccctaagga acggccctta ctcttagaac tcggaaccgc ctcgtttcgg
    30901 tttaaaaagg cgtgacgagc tgttggcaca gctgccataa tgagcttacg aatgcgctca
    30961 ccattataat ctgggtctgt caaaacaatg acaccacgca agtcatttag gcggttaatc
    31021 cgttctaaat cttcttcagt gatagcagag cctctggtct cataggtatc cacctcgtaa
    31081 aaacggcgaa ggttagccgt gtcgtccttg ccttctacaa caagaacctc ttgaatatta
    31141 attttttcag tcaagcttaa gtaccctctt tgcattagca gtcgtttctt ttgcaacttc
    31201 ttccacggtc attccccgta gctctgcgat tttatcaacc acataacggg tataagctgt
    31261 atgattttgt ttgccacgtt ttggaactgg tgtaagataa ggggcatctg tttccaccaa
    31321 aatcttatcc aaaggcaagt gctgcgctgc ctcttgaata tcaagggcct ttttgaaagt
    31381 gaccacacct gaaaaagaaa tcatcatgcc aagttcaatg aaacgttcag ccatctctaa
    31441 tgatcctgag taagaatgca tgatgcctcc acgagggcca acaccagctg ctttgatgac
    31501 ctcataggta tcttccaagg catcgcgggt gtgcaccaca aaaggcaaat catggtcttt
    31561 tgctaattgc atttggcgct taaaaacctc tatttgcacc tctttggggt cctccatcca
    31621 ataatagtct aagccaattt cacctaaggc aatgacttta ggatgtgata attgagagac
    31681 gatagactcc tcaactgctt cagaataaga tcctgcttct gtcgggtgcc atccaatagt
    31741 agcatacacg ttaggatact ggtctgcgag agcaagtgct ccactaatag tggcctggtc
    31801 aaagccaacc acattatggt aggccacgcc catttcttga gccaaagtca attcttcagt
    31861 ttcgtgtcct tgaaattctg ctacattcag gtgcgtatgg gtatcaaaaa tagttagttt
    31921 ttctacttta tttgtcatga tgttattata acataaagat cttaaccaca tcagaaaact
    31981 tggtgagaac ttctgccgtt taggttgatc gtaaaaccct ttgctagctg acacttgtct
    32041 attttctact tttttgacaa atgaccttta atagtcggct cctacataag ccctcaaaaa
    32101 tactacaacc tactcaaata aagtgggttg aacaaatctg tatcacaaaa agggtcgcta
    32161 ccttctgtta gcgatctatg ctatactagt agatagttaa actgtaagga gtatgctttg
    32221 ctatgcctaa aaaccaccgc ctaattacca aaatcgaagc atcattggaa catatgacca
    32281 gtttagaaaa agggattgct cattttttta ttacaaccga tctgacacct caagagttga
    32341 ccgcatccga gatagttaag cgactacaca tctctcaagc cgccttgact cgatttgcaa
    32401 aaaagtgtgg ttttactggt tatcgtgctt ttgcctttga ttaccttcac agtcttcaag
    32461 aatcacaaga gactttccaa tctattcact tagaattgac aaagcgcgtg ctgatggact
    32521 acgatgctct tattaataaa acctatgagc tcgttaatga agaaaaatta ctgaacttag
    32581 ccaagctgat cgatgcttct gagcgagtct atttctttgg caaaggatct tctggactag
    32641 tagcaagaga aatgaagcta cgctttatgc gacttggttt aatctgtgat gcttattcag
    32701 acactgatgg ttttacttgg gcaaatagcc tcgtcaatga aaattgcctt gtttttggtt
    32761 tttctttatc tggcaaaaca aactctgtca tcacagcact tcatcaagct agtcagcgag
    32821 gggccaaaac tgtcctctta acaacagata atcaaaccga atttgacgac tccttagata
    32881 ttattcccgt atcatcaacc caccaactac attatgggaa tcgtgtgtca ccacaatttc
    32941 ctcttcttat tatgatggac attatttatg cttacgtttt agcgattgac aaaccgcaca
    33001 aagaaaaaat cttcaaaaac actattattg acaaagaaaa taacggcctt tagactacaa
    33061 tagcaaaaag ggcgttgttt tctttttaaa gggtcgtcat tcacctcccc cagtatactt
    33121 aaacaagaac tcttcacgtc atatagactc acagccacta agattagcta ctcgctaaat
    33181 tattttcgat tttctcaaga gttataaatg aacaggatcg ctcattcttt tgtttaaaat
    33241 ggtagtaagc tcctaatata ccagcattat ttccatgact agcaaacttc agctgagttt
    33301 tcttggcaag actagaaacc aagtagctat caagagctgt ctttaattta tcagctaaat
    33361 agtctttttg caccatgatt ccgccaccaa gtaccaccac atttgggtta acaacgtagc
    33421 agatattagc aataccttgt cctaagtaat ccacctgctt actaatggct gctatacaaa
    33481 tcgcatcacc cgctttagcc tgctcaaaaa tacgacgacc atcccattgg ctaatgtcat
    33541 ccccataagc taaggccacc tcttgaacaa gagctgtggt agatgccaga tcctgaaact
    33601 gaccatctga caaatgaagg taaccaactt cgcaagctga atgactacta ccatgaaaaa
    33661 cttgggaatt aaacaataaa cagccaccga ttcccgtccc aattgtcaag caaagagcca
    33721 caggataatc cttggcactg ccagaaatag cttcggctag accagcacag ttaacatcgt
    33781 tttccacttc acacggtaac ccgaaagtct cttcaatctc ttttttaaac tgagttccag
    33841 catagttagg aatctgaggg ccggcataaa agatttctcc cttatcagga ttaaccattc
    33901 ctgcagaaga aatggccact ccagccagat ccatttggtc ttgataggtc tttaccaagc
    33961 ccttgacctt ttccaaaata gagggaccac ctttataagc ctcagtggcc atttcctctt
    34021 tttccaacag gtctcctgtt tcagaaatca agccatactt gatagcagta ccaccaatat
    34081 caattgctaa ataatgcttc atttgttatc ccctcgcatc ttattaaaag gtttccttag
    34141 cctgattaat cagagcagca gcccgttggc aaataaccct atcttcctcg accaactcag
    34201 ctaaaggtga tcgaacagaa ccaatatcaa gaccctcatt aatacgaagt acttccttaa
    34261 taaccccata catattgccg tgagcagaaa caaggacacc gatagtctca ttaatagtat
    34321 attgaagggt cttggccttt tctaaatcct tgtcaactat aagttgattc agccttaaaa
    34381 ataattctgg catagccccg taggtgccac caatgcctgc agcagcaccc atcaagcgcc
    34441 ctcctagaaa ttgttcatct gggccattaa aaacaatatg atcgtctccg ccaatcgcac
    34501 agaaggtttg aatatcctgc actggcatgg atgaattttt aacgccaatc acgcgcttat
    34561 tagccaacat agtcttataa aggcttgggg tcaaggcaac ccctgccagt tgaggaatat
    34621 tatagatgat aaaatctgtg tgaggagcag ccgaagaaat cgcattccaa taatcagcaa
    34681 ctgcatattc tggtaaccgg aaataaatag gcggaatagc tgcaatggca tccacgccca
    34741 aacgttctga atgagctgcc aattcaatac tatctttagt gttgttgcag gcaacgtgat
    34801 taataatcgt cagcttgccc ttagcgactg ccatgacatt ttctaacacc aattgacggt
    34861 caaaaacgct ctggtaaata cactctccag aagagccatt aatgtaaagc ccctgaaccc
    34921 ctttgtcaat atagtactgg gtcaaggcac gcacacgctc agggctaata gctccttggt
    34981 catcgtaaca agcataaaag gcaggaataa tgccttggta tttcgtaaga tctgtcatag
    35041 taatgtcctt tattcaatgt cattattaag tgggaatcgc ctaagaaggg attccatgat
    35101 aatcaagtag aggtaagtta ggctactgat gcccatagtg gccagtagag acaagccaca
    35161 aaataaggtt aacagggtta acggcaaggt aaaaaccaaa aggccaatca ccactagaaa
    35221 agcaaacgtc catgggaata aaattcctgc cagcagtaaa cttcgcttaa agagtattaa
    35281 aagaggaagc tcttttctga cggcctgagg ataagcgtac aaaaagagta taactaccaa
    35341 aaataaaaca gcaatgcaaa gtactttaaa cccttgaaaa actaggcctg attggtttcg
    35401 aattaagaaa agatctaaga cacaaatact cattatcaaa agctcaataa gacctaggcg
    35461 catcccactt ttaaaataat gtttaaaatg agagctataa gtagtaacaa gctgactaac
    35521 ttgaccttct cgccaatcca acaaactcgc atacaaagcc attttagcaa ccccaatcgt
    35581 tagaagggga aggcaggaca agacaaataa gagattaaac acgagcaaat caaacagtgc
    35641 tgcgctagcc ctcatccatt tactatcaag cttgaagaga gagtgtaaaa gtccctgttt
    35701 tttcgatgtc aaatcacaac ttcctttcta gaaatacgct tacaaacaaa agctaattcc
    35761 cctcttaaac gtttaacaca tgacttgctt cccatctgat ttatctatgt atgcacctaa
    35821 attaataagc aacctataaa ttaagcttag cgacacgctt tatttagtct aacctttcac
    35881 agcacccata gtaatgcctt gggtaaatga tttttggaag acaagaaata ccgtcacaat
    35941 aggcactgca gccatggcag cccctgccat aatcaagcca taattagtag ccatttcagc
    36001 ttgcatagtg gcaaccccaa gtgaaatcgt taaattttcc cgagaggtta acatcaccaa
    36061 ctgcataaaa taatcattcc aagtattgat aaaggtaaaa attgctagcg ctgcaaagcc
    36121 tggtttgaca atagggaaag ccacattgaa aaaggtacga atttcgccac atccatcaat
    36181 cttggcggat tccaataact cggttggaat attttctgag aactgtttca taaggaaaac
    36241 accaaatggc cagcccacaa gaggcaaaat aacagccgcc aaagtgtcat ggattcccat
    36301 aaaattaaca atccgcacaa gaggcactag aacaacctgt tttggcaagg ccatagcagc
    36361 aataaaaatc gaaaacaaaa gccgctgtcc ataaaaccgc tttttggcca aagcatagcc
    36421 agccaaagag gaagtgccac aaaccaagaa catggtcgcc accgaaataa agacactatt
    36481 ccacaaccat tttaaggctg gattttgcac taccaaggcc ttaaaatttt caatagtagg
    36541 tgcctttggc caccattgcg gtggaatcat aatggtatcg gcttgagcct taaatgctcc
    36601 tgtcataatc caataaaatg ggaaaataaa caaaatggtc aagatgcata gaataacagt
    36661 agtcagaaca tccgatgcgg ttagtttctt tttcgtcatc ttaatatcct cctaatattc
    36721 cacatcatta cctaaaattt taaattgagc aaagctgatg attgcaatca tgactgccaa
    36781 gaacacaccc attgtgttgg catagccgta ttcagagagc ttaaaggctt tttcatacaa
    36841 atagtacatg agggtactag ttgaataatt tggccctcca gacgtcaaga gttgaatcaa
    36901 agcaaaacat tggaaggaat taatggttgt tatgacagca atatataagg ttgttggcag
    36961 gagacttggc cacttgatat tccaaaacac ctgaaattct gtggcgccgt ctacacgcgc
    37021 agcttccatc aaagaattat caatatttcc catagcagca atatatagaa taataggctg
    37081 cccaacagat gtggttaata aaatagcaat aatagctaac aaagcccagt gcttatctcc
    37141 cagccaacta atgttttgtt caatcacatg agcatatttt aagacataat tcaaaatccc
    37201 tgacatcgga tcataaatcc atttccaaac caccgttacg gcaacacttc cggtaacaac
    37261 aggtagaaag aaaactgccc gataaaacga acgcgccacc acatttttgt cataagtctt
    37321 agccgccaca aagagggaaa agaaaactac aacaggtacc gaaccaataa cgataatcaa
    37381 cgtattgata agagatttca taaagatagg atcttgaaac atcctagcat aattggcaaa
    37441 accaacaaag gtaaaatccg tcatggaata attgaaaaaa ctcgtcacaa agcccataat
    37501 catcggtatc aagacaaaaa tcacaaagaa aaccaagacc ggagctaaga aagcgtatga
    37561 gatgagcgtc tctcgcattt ttaatttatt gacgttcact tgaaatacct cttttttctg
    37621 ttcaactttt ctaaaaagaa aagcttcttt ccagtgacca tttgacatgt gcattgaaaa
    37681 gaagccactt gttctgaaca cttattgtgt ttttttgatt gtcttgttcg ctttttcagt
    37741 gaaggctttc aaagcatctt ctggcttttc atcaccatta gagacggctt gaaccattgg
    37801 gaaccaaaga gttctcattt cagcaaaccc atcaatcgtg ttgtagtatg gtgagtagaa
    37861 ttttgtccat tcagcaattt tttccattcg tttgtctttg taaagatccc cataagaagt
    37921 tcttacaggg aaagcacctg tacgaacaac atttttaggt ccccattctt tatcatcagc
    37981 gataaactgg ataaatgtct tagaggcagc gactttttgt tcatctttgt tattaaagac
    38041 cgcaaaacca tttacgaggt attctagttc tggtttgcca tcatctgatg ggaatgggat
    38101 ttcaaggtaa tccactttac tagcttctaa caatttagct tggatgccgg gttgtgcagg
    38161 cgcccaaaga atggtaaagg aagtttggcc attcgcaaag ttttgaatat cagctgaacc
    38221 gtcgtattga gagccattca tcattaaacc atctttaatc caattggaaa ttttagtcat
    38281 ggctttaata gagttagcat catctgtggt gtatttggtt actttgtcat cggtaatatg
    38341 actactataa aggttagcaa agaaggcacg tggtccttgg tcaccacctt gaccattagc
    38401 aaagaaagaa cctgggttat atcctttgtc cttcaatgct ttcaagactt tttcaaaatc
    38461 atctgttgtc caaccctctt tcacaaggtc aaggacacca gcatctttga gcattttttt
    38521 attcaacgcc atgtagaatg gagcagagct gataggatac atgtaagctg tatctcctgc
    38581 ttttgaggct tgaatcaatt tgtcattgtt aacatctttg gtaaactctt ctgtgaacaa
    38641 gtcattcaag tcagccaatt tcccattttt accatattga atgatacgac ctggagcatc
    38701 aaagaggaca tcaggagctg ttccagcttc aatagcagtt gtgattttct caggaccaga
    38761 tgtaaaatca atggtttcca attttacctt gatttctgga ttagctttct caaaggcagc
    38821 aatcaatttc ttctcatagg ttcccacacc atcttcagct ttttcttgcg tgaagaccgg
    38881 gaaagcccac catgtgattt ctgtcttagc agtatcagat ttacttgcac cagcctcttt
    38941 ctggctcttt ccgccacaag ctgctagtcc taaaacagat gcaccaagca tcactaatga
    39001 agctaacttc ttcatgttca tcgtaaaatc tccttttttt gatgtgtgtt tctattttga
    39061 caaccactat taggatttga gcgcttcgat aaaacgctct gcaatttcct taggccttgt
    39121 aatggcccct ccaacaacaa tacctgccac acctaagtca ttaattttct tggcttcttc
    39181 tggggaatga atctttcctt cggcaatgac agcaatgcct gctttgcaaa gagcttctat
    39241 caatgccaca tcaggacctg cttcctgacg gctatacggg gtgtagcctg acaaggtggt
    39301 tccaacaaaa tcaatgccag cctgatgggc cactagtcct tcatcaaagg tactaatatc
    39361 tgccatcaaa agctgattgg ggtacttttc cttaacttgt cgaataaagc tagcaatatc
    39421 caagccatca tgtcggtcac gcttggtaca atccatagca atcactgcaa tatttaaggc
    39481 agctaattga tccacttcgg ccatcgttgc cgtaataaaa ggctcttgcg gcgggtaatc
    39541 ttttttgatg ataccgataa ttggtaggtc tgttatggct tgaatttctt taatgtctct
    39601 aaccgaatta gctctgatgc cgacagcacc agcttcttga gcagcttttg ccagcagtgg
    39661 cataataccc ccagtctctg aatagagagg ctcacctggc aaagcctgac aagaaacgat
    39721 aatcccaccc tttaactgct ccatgagctt ttctttggtt ggtttatcag gcatagtttc
    39781 tactccttgt gtttcttagt tttaataaaa ataacgttca caaagttatt ataacaaaaa
    39841 ataaagcgct ttcaatagca atgagacttt tagaatctac tttcattttt tatattattt
    39901 tctattattt ggaatgctct ttcatacaac aaataaaaag gtaaggagtt gacaccatac
    39961 ttatacattg caaattcccg attagaaaac gttaaaaaac caagagcctc gagcactctt
    40021 ggtttacttt agttgtattt tttgcaatta agctgataat actttacgtc ctttacgacg
    40081 acgtgcagca agcacgcgac gtccgttttt agttgacata cggtgacgga atccgtgttt
    40141 acgttgacga cggattttac ttggttgata agtacgtttc acagtgaata cctcctcata
    40201 gattttgtat tcgtttagcc ggctatttgt gatatcagtt actaaacata ctataatatt
    40261 ctatcttaaa gagggtctac tgtcaagatt tattttcgtt tttcaacagt ttctctctca
    40321 tttaagaggc cgctcatttc ataaagtgtc tcttctttga cactgttcct ttgccttacc
    40381 gttgaaacct tatcaatagc aacaataatc aatgagatca gtaaaacaaa cggttaagct
    40441 ggccatacaa aaaatcttcg atagctaaac taactaccaa agattctttt taggatctaa
    40501 cgttgaagac tgactaccac atagcggtta gggtcattgc cttcagaata actatcaacc
    40561 ccttcaatgg atgaaacggt tttatgaacg attttacgct cactgttact catgggatcc
    40621 atggtgtaat cttgaccaga ttccaagacg cgtttggcaa ctttttgcgt aaagtcaatc
    40681 aaggtttctg ttcgatgttc cacataatca tggacattca aggatactga aaaatttttt
    40741 gaatagcggt cgtgcaagaa attttgagct aacaactgca aggatttcaa aaccttacca
    40801 tggtaaccaa tcacacgtcc cgcctctggc gtttcaatct gcaaattgat ttggcgacga
    40861 ttgttgctgg tttcaacggt ggcttcgata tccatttcat agatgatttt agtcacataa
    40921 tcggacactt cctgagcagc tttttcaata tctttgctga aaccatcatt accaggatct
    40981 tggggcactt ctgattcctc gactgggatt gacggttgtt gtgcttttgt ttcagtgact
    41041 gtctcagcaa gattagaaag ggtttgcgct ggactatctt cagacatgag aggaaccact
    41101 ttggtatctt ctgcttctaa cttttgagtg gcttttattt cttcaggttc tacatcagca
    41161 gaattgaccg cgggagcatt tttttgatta atatcctctg gaacacctcg agtggctttt
    41221 ttatcagcct tataaaccgt cttgtcgctg ataccttcga tatcaacttg ggctggcttt
    41281 ttaccgaacc cgaggaaacc ttttttctct tttgaaatga ctttgatatg cgctttaagg
    41341 cgtgataacc ctaactcttg aagtcctgtt tcgatagctt cttcgactgt ttttcctgta
    41401 aataatacca taacctaaac tcctattttc tcttcattgc ttttttacga gcacgacgtt
    41461 cacgaagacg gcgttctttt tcttcattgg caagacgctg tctttcggca ataatcttaa
    41521 aaggattgtt taatagcagt agttgtacca cttgaaaggc gtttgagacc gtccaataga
    41581 gcactactcc actagccaag ttaaagccca tgaaaaagat cattagcggc atcacataaa
    41641 tcataacagt catcatgaca tttttttctt tagccgctag attggtcaac caagtggaca
    41701 agaaagtgaa gacagctgcc aaaactggta aaaggtacaa atgatcatgt tgtgccaatt
    41761 ccacccacaa gaaagtccct gtttttaaga aagatacccg tgtcaaggct tggaataggg
    41821 caatcataac tggcatctga attaagaggg gtaagaggct agcataaggg ttaaccccat
    41881 attttttgta aagggcttga ctttcttcag ccaacttcat ccgcgtttgt gtgtctttac
    41941 cagcgtattt cttttgcaat tctctaagct caggttggat gtcttgcatc ttctggcttg
    42001 atttgatttg catattaaac aaaggcatga gcatgagacg gatagtaagg gtaaaaagaa
    42061 taatgccaac accaattgaa ccatcaaagg aaagccattg aattgctctg gcaaataagt
    42121 aaaccaactg gtcccaacca ctagatgatt gtgccgttac ctcaccacga ccacaggcca
    42181 ctaataatag aactaagggg actattcttg caattttaat gtttttcttc actctcaaaa
    42241 cctttctcaa gcaattgtgc taactttaaa acatgatgta aattttgttg aagctcttga
    42301 tactccaaag actcgacacc cttacgggca atcaccacga aatcctctga cttcagctga
    42361 tgccctaatg ccatgataac atgacgtatc tttcgtttga ctgcatttct ggtgactgca
    42421 tttcctattt ttttaccgac agaaataccc acacggaagt ggtcttggcc tctatttaaa
    42481 tgataaatga caaattttcg atttgctgta ctttttccat ccttaaatat ggcttggaaa
    42541 tctttctcac gcttgacacg ataggtcttc ttcaaaattt aactccaata tctaaattat
    42601 taccattata ccacatcccc taaaaaatta aaaagcccta accctttcag attaagcctc
    42661 tttctgtact atttctagtt tttgtgcttc aactgcctct tgtcactcca tcttttcgta
    42721 gtatagttct tctttcgaca aaggaataac cttcagaaaa caaccataaa aagtcagaat
    42781 ccacaagacg tcagctactt atccctgtat ttacctatca aaaaacccgg caagccgggc
    42841 tttgatactg attttacaat cagctaatgt gatatccaaa caaagacaac tgacatcgtc
    42901 attttgtctg ccgagccatc atatcaaaaa tactatttgt ttatggagag agagaaaaca
    42961 atattttgat tgaattgggg attgattgct cgataaactc atgacttcgt aaatctttat
    43021 ttacactaat tattatagcg aaaaatgttt caataagagc atcatttcct gaaatgttgc
    43081 tttttcgtcc taaaatgtta ttgctctctt aactttagcg aagagcctcc caccgttcaa
    43141 acagacattt catttttaat cgtgatacca tacaagaatc accattttcg aaataaagga
    43201 gctgcttact cttatcaatt cgaacaacct tatctagatt cacaaggtag gatctatggc
    43261 acaaaaaaag tttcggtgct actgcttgaa tctcagatag attaccataa aactctaatc
    43321 tttctgtctg agtccataaa caaactttgt gaggagttgt ggctgtagca aagtataaaa
    43381 tatccttata gggcaatttt aatcttgtct gtggagtctc aaataaaaac atgtcctttg
    43441 actcacgact agacatcata tcgtaagtat atcggataca ttcttcgatt tgttctttaa
    43501 actgtttttg accacctgct ttatcaataa aatctagagc ggatacctta tatttaaaag
    43561 aaataggagc aaattctgaa tgagtggtga caaacacaat aacggcatta ggatcttttt
    43621 gtcgaatagc tgcagctaat tcaagcccac aacgagtata ttcaccaatt tcaatatcta
    43681 aaaaatacag ctgatggtct cctctttctt ggatactctc aaataatttt tgaggagtcg
    43741 agaacacttc aagctgatta catggtattc tagtctcttt taagatgcct actacgatag
    43801 attctatgcg tgtctgctgg ataaaatcat cctcaagaat aaaaatgttc atcatacttt
    43861 atctcatttc taacaattgt cttaatcgat aatggtcact tttagtcgaa agcgttattt
    43921 gggggtaact atctaaaaca gtcaaaaagt gggctgagtt ctgctgctga gcatcaaagc
    43981 gtttggctat atccacacga ttggtttttg tgctattttc aataataaat aattgcttgt
    44041 tatcttgttt aaagtatgca atagataaaa aaggacgtct actgcctttg gctgtctcaa
    44101 tagcattgtc cactagtcca gtaaaaagta caattaagtc gattaactta ataggtaaat
    44161 gatcgataac atctggaatc tcaatataaa gttcaatacc caaatactga gcttcaagca
    44221 atttggaatt catgatactg cgaatcacag cctctttgat attgtctaaa gaagatattt
    44281 cataatttgt gcgcatcata gaagttgccg attgctgaat aaccgtgtca taaactctag
    44341 taatcagatc tatatcacca ctatcgatac tatcttttag actaattaag atattttcag
    44401 aatcatgttt aactgttctg atttctcggt aaagttgctc aatcgattga ttgtaatttt
    44461 ccagatacgc aatacgttca ttttgtgctt gtgttaattt ttgtgacagc tgatctttag
    44521 caaatcgatc taattttaca ataacccaaa taaaaatagc taaataaata aatactaaaa
    44581 tagaccggta ctggaaatat agtgcaagaa atccgctttg cacagttacc aaaaaatggg
    44641 cgaaaaagta gtaagaaaac atggcagtat tcatccaaaa tacccacttt ttcatcttat
    44701 cttcactgat aaaccttatt aagcttaagt caatactgaa aatgtagcta aacagataaa
    44761 atattggtaa tacaaaaaga taacataact ctaaaagctt aaggttatta ttaatttgac
    44821 caatgctaaa tcctaagaca gctggtaaaa agaaagatcc gatgacgcga aacagtaatt
    44881 cgacaatcaa gatagtaaag aaaccattaa acatatgctc aaataatttc tttttcggag
    44941 cacttaacaa actaacaata ataaaaaaca gttggtcaat caaaatgact ttgtcaaata
    45001 tcatattagc aattacaaaa ctaatgccta taacgctaac ccgtttccag cttagtttaa
    45061 tagcagaaac ctttgcaaaa atgacccagc tgttaataaa gatggtagca tagaataaag
    45121 ccatactgta tataaccatc atctatccac ctcctccttt ggcatggtca aggtttggct
    45181 aaattcctta tcaaaacttt tagtccgcaa ggataaataa ggatactttt tgagaatacg
    45241 ctgcgcatta gaaaggccga taccacgttc tgaacctttt gaagaatagc cttcttcaaa
    45301 gatcttgtta atgtctacct tttttttagt cgtatttgtc acaacaaaca tctgatagtc
    45361 atctaacaaa aagtaagcaa ttgacatatg aggttgctgt gcttcaagag ctgcctcaat
    45421 ggcgttatca caaaaaatag acatcaacaa tagtaagtcc aactcaggaa tgtaagtctc
    45481 ttttatatta tctggcactt ccacattcaa atcaatacct gatttttccg cactgataat
    45541 tttagctgac aataagctct taatcgatga gatattaatt ttcctaagct tcgagatatt
    45601 atagtgttta tcatcccagt agtcgttagc ttcatgaacc gtttgggcat aaatatcttg
    45661 aatctgagta atggattcac tcttaatggc ttgacctaaa cgctctatac ggctcaggta
    45721 atcactttga aaagctctta catctcgata gagtttctca agatgtttcc cataagtctc
    45781 taagttagtc atataagctt gttgttctct tctcaactca tttttaaggt attctttaga
    45841 actatgatta aaataagata gaagataaac taaaagaagt ataaacatca aaaacaataa
    45901 cttataaggt gtgggaacaa aaagagaatg atgaaaatgt aaaaaggggt ccgtaaatcc
    45961 taaaatgaca aaaatgtcca cataataaac caaaacaaaa atcagcaatg tttgataaaa
    46021 attatgcgat cgtttcgaat atccggattg gcatatcatc ttgtaatctt ttccaatcat
    46081 ataattaata atagcaaaac ttggaaaaat cagcagataa gcaagcaagt caaagataat
    46141 atgacccaga taaataccac tcgacaaaaa aagatagggg ataacgattg tacccataaa
    46201 ccgagatagc aaatccacca aaacactagg tgcaacagcc aaaaaaagag ctttcaaaga
    46261 aaataattgc ttggtttctt gccttaaaac aactaaaaat aatagaggat caattagcaa
    46321 tggcgacaaa tacgggaact gattgaaaaa tagggctaaa gccaaaatcg ttagttgttt
    46381 ccaaactggt aatgtgatat cagatagaaa ggaaaaaata taaatttttg
//
LOCUS       DATXIG010000009        46349 bp    DNA     linear   BCT 28-DEC-2023
DEFINITION  TPA_asm: Streptococcus pyogenes strain MGAS11113
            SAMN08555799-rid19682583.denovo.009, whole genome shotgun sequence.
ACCESSION   DATXIG010000009 DATXIG010000000
VERSION     DATXIG010000009.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN08555799
            Sequence Read Archive: SRR6740495
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pyogenes
  ORGANISM  Streptococcus pyogenes
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 46349)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 46349)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-OCT-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-OCT-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001918058.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001918058.2
            Genome Coverage       :: 368x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/12/2023 11:23:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 1,806
            CDSs (total)                      :: 1,769
            Genes (coding)                    :: 1,722
            CDSs (with protein)               :: 1,722
            Genes (RNA)                       :: 37
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1, 1 (5S, 23S)
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 47
            CDSs (without protein)            :: 47
            Pseudo Genes (ambiguous residues) :: 0 of 47
            Pseudo Genes (frameshifted)       :: 30 of 47
            Pseudo Genes (incomplete)         :: 20 of 47
            Pseudo Genes (internal stop)      :: 9 of 47
            Pseudo Genes (multiple problems)  :: 11 of 47
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..46349
                     /organism="Streptococcus pyogenes"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN08555799-rid19682583.denovo.009"
                     /strain="MGAS11113"
                     /serotype="Emm28"
                     /isolation_source="normally sterile site"
                     /host="Homo sapiens"
                     /db_xref="taxon:1314"
                     /country="Finland"
                     /collection_date="2002"
                     /collected_by="National Public Health Laboratory"
     gene            47..119
                     /locus_tag="VC122_000501"
     tRNA            47..119
                     /locus_tag="VC122_000501"
                     /product="tRNA-Phe"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:80..82,aa:Phe,seq:gaa)
     gene            139..219
                     /locus_tag="VC122_000502"
     tRNA            139..219
                     /locus_tag="VC122_000502"
                     /product="tRNA-Tyr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:173..175,aa:Tyr,seq:gta)
     gene            226..296
                     /locus_tag="VC122_000503"
     tRNA            226..296
                     /locus_tag="VC122_000503"
                     /product="tRNA-Trp"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:258..260,aa:Trp,seq:cca)
     gene            308..380
                     /locus_tag="VC122_000504"
     tRNA            308..380
                     /locus_tag="VC122_000504"
                     /product="tRNA-His"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:341..343,aa:His,seq:gtg)
     gene            389..460
                     /locus_tag="VC122_000505"
     tRNA            389..460
                     /locus_tag="VC122_000505"
                     /product="tRNA-Gln"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:421..423,aa:Gln,seq:ttg)
     gene            480..563
                     /locus_tag="VC122_000506"
     tRNA            480..563
                     /locus_tag="VC122_000506"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:514..516,aa:Leu,seq:caa)
     gene            823..1008
                     /locus_tag="VC122_000507"
     CDS             823..1008
                     /locus_tag="VC122_000507"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002987757.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEP5640453.1"
                     /translation="MSFEIFTLIIGWKLVGYRVMECGSSFKQGDYRLVVHICLLNVRD
                     EMLSQQGHEDKADWPEP"
     gene            1646..1954
                     /locus_tag="VC122_000508"
     CDS             1646..1954
                     /locus_tag="VC122_000508"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011284415.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-diphosphocytidyl-2C-methyl-D-erythritol
                     kinase"
                     /protein_id="HEP5640454.1"
                     /translation="MKHRFQVKRGVSVYLEKRIPMCAGMGGGSSDAVTIRALNQLWLL
                     TLSRKDMMDIGIPIGSDVPYCLLSGRAQVTGKGEVVCRILGLLSSWVVLVKPDFGIST
                     "
     gene            2009..2221
                     /locus_tag="VC122_000509"
     CDS             2009..2221
                     /locus_tag="VC122_000509"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002993461.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-diphosphocytidyl-2C-methyl-D-erythritol
                     kinase"
                     /protein_id="HEP5640455.1"
                     /translation="MVAAIEAGNYNDGILTEMNNLLEDIFIAKRPFIQKIKEKTLQAG
                     AATTLMTGSGPTVFALCQTEKQTIIV"
     gene            2331..2774
                     /locus_tag="VC122_000510"
     CDS             2331..2774
                     /locus_tag="VC122_000510"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002986587.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc-dependent MarR family transcriptional
                     regulator"
                     /protein_id="HEP5640456.1"
                     /translation="MGTLEKKLDNLVNTILLKAENQHELLFGACQSDVKLTNTQEHIL
                     MLLSQQRLTNTDLAKALNISQAAVTKAIKSLVKQDMLAGTKDTVDARVTYFELTELAK
                     PIASEHTHHHDETLNVYNRLLQKFSAKELEIVDKFVTVFAEELEG"
     gene            2778..3497
                     /locus_tag="VC122_000511"
     CDS             2778..3497
                     /locus_tag="VC122_000511"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002986585.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal ABC transporter ATP-binding protein"
                     /protein_id="HEP5640457.1"
                     /translation="MRYISVKNLSFQYESEPVLEGITYHLDSGEFVTMTGENGAAKST
                     LIKATLGILQPKAGRVTIAKKNKDGKQLRIAYLPQQVASFNAGFPSTVYEFVKSGRYP
                     RSGWFRHLNKHDEEHVQASLEAVGMWENRHKRIGSLSGGQKQRVVIARMFASDPDIFV
                     LDEPTTGMDSGTTDTFYELMHHSAHQHGKSVLMITHDPEEVKAYADRNIHLVRNQKLP
                     WRCFNIHEVETDDEKGGHGHA"
     gene            3490..4305
                     /locus_tag="VC122_000512"
     CDS             3490..4305
                     /locus_tag="VC122_000512"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002987764.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal ABC transporter permease"
                     /protein_id="HEP5640458.1"
                     /translation="MLDILSYDFMQRAVMAVVAISIFAPILGIFLILRRQSLMSDTLS
                     HVSLAGVALGVVLGISPTITTIIVVVLAAILLEYLRVVYKHYMEISTAILMSLGLALS
                     LIIMSKSHSSSSMSLEQYLFGSIITISMEQVVALFAIAAIILILTVLFIRPMYILTFD
                     EDTAFVDGLPVRLMSVLFNIVTGVAIALTIPAAGALLVSTIMVLPASIAMRLGKNFKT
                     VILLGIVIGFSGMLSGIFLSYFFETPASATITMIFISIFLLVSLGGMLKKRLF"
     gene            complement(4345..4728)
                     /locus_tag="VC122_000513"
     CDS             complement(4345..4728)
                     /locus_tag="VC122_000513"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011528158.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HIT family protein"
                     /protein_id="HEP5640459.1"
                     /translation="MTECIFCHQLKANQLLTQSQYFKVVFDIDPIQKGHLLLISKDHY
                     TSLTQLPKEVRYDLIDLQAALVAKLEQHLPISGVTSVSNDKELMDEGTHFHLHLIPRL
                     TNDSFWESITINQENWDLTPFLKHL"
     gene            complement(4779..6035)
                     /locus_tag="VC122_000514"
     CDS             complement(4779..6035)
                     /locus_tag="VC122_000514"
                     /EC_number="6.1.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011184085.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine--tRNA ligase"
                     /protein_id="HEP5640460.1"
                     /translation="MNIFEELKARGLVFQTTDEQALVKALTEGQVSYYTGYDPTADSL
                     HLGHLVAILTSRRLQLAGHKPYALVGGATGLIGDPSFKDAERSLQTKETVLEWSDKIK
                     GQLSTFLDFENGDNKAELVNNYDWFSQISFIDFLRDVGKYFTVNYMMSKDSVKKRIET
                     GISYTEFAYQIMQGYDFYELNDKHNVTLQIGGSDQWGNMTAGTELLRKKADKTGHVMT
                     VPLITDSTGKKFGKSEGNAVWLDADKTSPYEMYQFWLNVMDDDAVRFLKIFTFLFLDE
                     IAEIETQFNAARHERLAQKTLAREVVTLVHGEEAYKQALNITEQLFAGNIKNLSANEL
                     KQGLSNVPNYHVQSEDSLNLVDMLVTAGISPSKRQAREDVQNGAIYINGDRVQDLDYQ
                     LSNDDKIDDQLTVIRRGKKKYAVLTY"
     gene            6127..8439
                     /locus_tag="VC122_000515"
     CDS             6127..8439
                     /locus_tag="VC122_000515"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002987769.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin-binding protein"
                     /protein_id="HEP5640461.1"
                     /translation="MRYFMVKWNTKQKRISHQRLGLLDLGPVLLRTLRLLSNFFYIVI
                     FLFGMMGFGMAFGYLASQIESVKVPSKESLVKQVESLTMISQMNYSDNSLISTLDTDL
                     LRTPVANDAISENIKKAIVSTEDEHFQEHKGVVPKAVFRATLASVLGFGEASGGSTLT
                     QQLVKQQVLGDDPTFKRKSKEIVYALALERYMSKDNILCDYLNVSPFGRNNKGQNIAG
                     VEEAARGIFGVSAKDLTVPQAAFLAGLPQSPIVYSPYLSTGQLKSEKDMAYGIKRQQN
                     VLYNMYRTGVLSKKEYEDYKAYPIQKDFIQPGSAIVNNHDYLYYTVLADAKKAMYSYL
                     IKRDKVSSRDLKNDETKAAYEERALTELQQGGYTITTTINKPIYNAMQTAAAQFGGLL
                     DDGTGTVQMGNVLTDNATGAVLGFVGGRDYALNQNNHAFNTIRSPGSSIKPIIAYGPA
                     IDQGLMGSASVLSNYPTTYSSGQKIMHADSEGTAMMPLQEALNTSWNIPAFWTQKLLR
                     EKGVDVENYMTKMGYKIADYSIESLPLGGGIEVSVAQQTNAYQMLSNNGLYQKQYIVD
                     KITASDGTVVYKHENKPIRIFSAATATILQELLRGPITSGATTTFKNRLAAINPWLAN
                     ADWIGKTGTTENYTDVWLVLSTPKVTLGGWAGHDDNTSLAPLTGYNNNSNYLAYLANA
                     INQADSNVIGVGQRFNLDPGVIKANVLKSTGLQPGTVNVNGHTFSVGGEMTTSLWSQK
                     GPGAMTYRFAIGGTDADYQKAWGNFGFRKN"
     gene            8703..12269
                     /gene="rpoB"
                     /locus_tag="VC122_000516"
     CDS             8703..12269
                     /gene="rpoB"
                     /locus_tag="VC122_000516"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003055252.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit beta"
                     /protein_id="HEP5640462.1"
                     /translation="MAGHEVRYGKHRTRRSFSRIKEVLDLPNLIEIQTDSFQDFLDSG
                     LKEVFEDVLPISNFTDTMELEFVGYEFKEPKYTLEEARIHDASYSAPIFVTFRLVNKE
                     TGEIKTQEVFFGDFPIMTEMGTFIINGGERIIVSQLVRSPGVYFNDKVDKNGKVGYGS
                     TVIPNRGAWLELETDSKDIAYTRIDRTRKIPFTTLVRALGFSGDDEIVDIFGESDLVR
                     NTIEKDIHKNPSDSRTDEALKEIYERLRPGEPKTADSSRSLLIARFFDARRYDLAAVG
                     RYKVNKKLNIKTRLLNQIIAENLVDAETGEILVEAGTEMTRSVIESIEEHLDGDLNKF
                     VYTPNDYAVVTEPVVLQKFKVVSPIDPDRVVTIVGNANPDDKVRALTPADILAEMSYF
                     LNLAEGLGKVDDIDHLGNRRIRAVGELLANQFRIGLARMERNVRERMSVQDNDVLTPQ
                     QIINIRPVTAAVKEFFGSSQLSQFMDQHNPLSELSHKRRLSALGPGGLTRDRAGYEVR
                     DVHYTHYGRMCPIETPEGPNIGLINNLSSFGHLNKYGFIQTPYRKVDRATGRVTNEIV
                     WLTADEEDEYTVAQANSKLNEDGTFAEEIVMGRHQGNNQEFSASVVDFVDVSPKQVVA
                     VATACIPFLENDDSNRALMGANMQRQAVPLIDPKAPYVGTGMEYQAAHDSGAAVIAQH
                     NGKVVFSDAEKVEIRRQDGSLDVYHITKFRRSNSGTAYNQRTLVKVGDIVEKGDFIAD
                     GPSMENGEMALGQNPVVAYMTWEGYNFEDAVIMSERLVKEDVYTSVHLEEFESETRDT
                     KLGPEEITREIPNVGEEALKDLDEMGIIRIGAEVKEGDILVGKVTPKGEKDLSAEERL
                     LHAIFGDKSREVRDTSLRVPHGGDGIVRDVKIFTRANGDELQSGVNMLVRVYIAQKRK
                     IKVGDKMAGRHGNKGVVSRIVPVEDMPYLPDGTPVDIMLNPLGVPSRMNIGQVMELHL
                     GMAARNLGIHIATPVFDGASSEDLWDTVREAGMDSDAKTVLYDGRTGEPFDNRVSVGV
                     MYMIKLHHMVDDKLHARSVGPYSLVTQQPLGGKAQFGGQRFGEMEVWALEAYGASNVL
                     QEILTYKSDDVTGRLKAYEAITKGKPIPKPGVPESFRVLVKELQSLGLDMRVLDEDDN
                     EVELRDLDEGEDDDIMHVDDLEKAREKQAQETQEVSETTDEK"
     gene            12360..16001
                     /gene="rpoC"
                     /locus_tag="VC122_000517"
     CDS             12360..16001
                     /gene="rpoC"
                     /locus_tag="VC122_000517"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020904830.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit beta'"
                     /protein_id="HEP5640463.1"
                     /translation="MVDVNRFKSMQITLASPSKVRSWSYGEVKKPETINYRTLKPERE
                     GLFDEVIFGPTKDWECACGKYKRIRYKGIVCDRCGVEVTRAKVRRERMGHIELKAPVS
                     HIWYFKGIPSRMGLTLDMSPRALEEVIYFAAYVVIDPKDTPLEPKSLLTEREYREKLQ
                     EYGHGSFVAKMGAEAIQDLLKRVDLAAEIAELKEELKSASGQKRIKAVRRLDVLDAFN
                     KSGNKPEWMVLNILPVIPPDLRPMVQLDGGRFAASDLNDLYRRVINRNNRLARLLELN
                     APGIIVQNEKRMLQEAVDALIDNGRRGRPITGPGSRPLKSLSHMLKGKQGRFRQNLLG
                     KRVDFSGRSVIAVGPTLKMYQCGVPREMAIELFKPFVMREIVAKEYAGNVKAAKRMVE
                     RGDERIWDILEEVIKEHPVLLNRAPTLHRLGIQAFEPVLIDGKALRLHPLVCEAYNAD
                     FDGDQMAIHVPLSEEAQAEARLLMLAAEHILNPKDGKPVVTPSQDMVLGNYYLTMEDA
                     GREGEGMIFKDKDEAVMAYRNGYAHLHSRVGIAVDSMPNKPWKDSQRHKIMVTTVGKI
                     LFNDIMPEDLPYLQEPNNANLTEGTPDKYFLEPGQDIQEVIDRLDINVPFKKKNLGNI
                     IAETFKRFRTTETSAFLDRLKDLGYYHSTLAGLTVGIADIPVIDNKAEIIDAAHHRVE
                     EINKAFRRGLMTDDDRYVAVTTTWREAKEALEKRLIETQDPKNPIVMMMDSGARGNIS
                     NFSQLAGMRGLMAAPNGRIMELPILSNFREGLSVLEMFFSTHGARKGMTDTALKTADS
                     GYLTRRLVDVAQDVIIREDDCGTDRGLLIRAITDGKEVTETLEERLQGRYTRKSVKHP
                     ETGEVLIGADQLITEDMARKIVDAGVEEVTIRSVFTCATRHGVCRHCYGINLATGDAV
                     EVGEAVGTIAAQSIGEPGTQLTMRTFHTGGVASNTDITQGLPRIQEIFEARNPKGEAV
                     ITEVKGNVVEIEEDASTRTKKVYVQGKTGMGEYVVPFTARMKVEVGDEVNRGAALTEG
                     SIQPKRLLEVRDTLSVETYLLAEVQKVYRSQGVEIGDKHVEVMVRQMLRKVRVMDPGD
                     TDLLPGTLMDISDFTDANKDIVISGGIPATSRPVLMGITKASLETNSFLSAASFQETT
                     RVLTDAAIRGKKDHLLGLKENVIIGKIIPAGTGMARYRNIEPQAMNEIEVIDHTEVSA
                     EAVFTAEAE"
     gene            16153..16518
                     /locus_tag="VC122_000518"
     CDS             16153..16518
                     /locus_tag="VC122_000518"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002986560.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1033 family protein"
                     /protein_id="HEP5640464.1"
                     /translation="MYQVIKMYGDWEPWWFIDGWQDDIIDEQQFSDWQEALDYFNQEW
                     QRMKAIFPSYHSQKNLLATFWEKEDKRWCEDCDEDLQQFHSLLLLKNKDIVPSNNYIP
                     EFEQRNDSPQVAYLCKLNL"
     gene            16611..17549
                     /gene="tadA"
                     /locus_tag="VC122_000519"
     CDS             16611..17549
                     /gene="tadA"
                     /locus_tag="VC122_000519"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009880361.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Flp pilus assembly complex ATPase component
                     TadA"
                     /protein_id="HEP5640465.1"
                     /translation="MVQALAKAILAKAEQVQAQDIYILPRADQYDLFLRIGDERRLVD
                     VYQSDRMAPLISHFKFVAGMIVGEKRRCQVGSCDYKLSKDKQLSLRLSSVGDYRGQES
                     LVIRLLHHQNKSVHYWFDGLTKVANQVGGRGLYLFAGPVGSGKTTLMYQLISSYHQEA
                     QVISIEDPVEIKNHQILQLQVNDDIGMTYDNLIKLSLRHRPDILVIGEIRDSQTARAV
                     IRASLTGAMVFSTVHAKSISGVYVRLLELGVTKAELSNCLALIAYQRLLNGGALIDST
                     QNEFEYYSSSNWNQQIDQLLEAGHLNPKQAKLEKII"
     gene            17533..18519
                     /locus_tag="VC122_000520"
     CDS             17533..18519
                     /locus_tag="VC122_000520"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002986555.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system F family protein"
                     /protein_id="HEP5640466.1"
                     /translation="MKKLSSKHQHKFIQLLANLLSTGFSFAEVIAFLKRSQLLQLDYV
                     LKMEESLLKGQDLADMLSGLGFSDAILTQISLADRHGNIETTLVAIQHYLNQMARIRR
                     KTVEVITYPLILLLFLFVMMLGLRRYLVPQLETQNQITYFLNHFPAFFIGFCSGLILL
                     FGMVWLRWRSQSRLKLYSRLSRYPFLGKLLKQYLTSYYAREWGTLIGQGLDLMTILDI
                     MAIEKSSLMKELAEDIRMSLLEGQAFHIKVATYPFFKKELSLMIEYGEIKSKLGAELE
                     IYAQESWEQFFSQLYQVTQLIQPAIFLVVAVTIVMIYAAILLPIYQNMGGIF"
     gene            18521..18847
                     /locus_tag="VC122_000521"
     CDS             18521..18847
                     /locus_tag="VC122_000521"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002986552.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prepilin-type N-terminal cleavage/methylation
                     domain-containing protein"
                     /protein_id="HEP5640467.1"
                     /translation="MINQWNNLRHKKLKGFTLLEMLLVILVISVLMLLFVPNLSKQKD
                     RVTETGNAAVVKLVENQAELYELSQGSKPSLSQLKADGSITEKQEKAYQDYYDKHKNE
                     KARLSN"
     gene            18822..19250
                     /locus_tag="VC122_000522"
     CDS             18822..19250
                     /locus_tag="VC122_000522"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011184089.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system protein"
                     /protein_id="HEP5640468.1"
                     /translation="MKKPVLAIKAFTLLETLLSLSVMSFIILGLSVPVTKSYQKVEEH
                     LFFSHFEHLYRHQQKLAILQQKQRVLDISSTKIVTEGNSLTVPKSITVNHPYRLVIDQ
                     MGGNHSLAKIIFDMTDRRFKYQFYLGSGNYQKTSQSLHSP"
     gene            19255..19491
                     /locus_tag="VC122_000523"
     CDS             19255..19491
                     /locus_tag="VC122_000523"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011888535.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="competence protein ComG"
                     /protein_id="HEP5640469.1"
                     /translation="MIATGILFSIVILVLSSLQQSQAALTYYRKQQEKLNLALMAVQT
                     RTKEITLNGCHITILRTDRYISIHDDEGEVMKIE"
     gene            19484..19918
                     /locus_tag="VC122_000524"
     CDS             19484..19918
                     /locus_tag="VC122_000524"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014407215.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="competence protein ComGF"
                     /protein_id="HEP5640470.1"
                     /translation="MSKQLSNIKAFTLLEALIALLVISGSLLVYQGLTQTLLKRSHYL
                     ARHDQDNWLLFSHQLREELSGARFYKVADNKLYVEKGKKVLAFGQFKSHDFRKSASNG
                     KGYQPMLFGISRSHIHIEQSQICITLKWKSGLERTFYYAFQD"
     gene            19902..20228
                     /locus_tag="VC122_000525"
     CDS             19902..20228
                     /locus_tag="VC122_000525"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002986539.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="competence protein ComG"
                     /protein_id="HEP5640471.1"
                     /translation="MPFKTNLKAGILLYAMFMASIFLLVLQVYLSQVTALHKEYQAQT
                     DYVKARLIAEIVYQDHRYKASNPVFFKGGQVICRERKERWMLIVKLDQQRQYQFEYLK
                     PKQYSQ"
     gene            20326..21279
                     /locus_tag="VC122_000526"
     CDS             20326..21279
                     /locus_tag="VC122_000526"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014407216.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HEP5640472.1"
                     /translation="MTFEKIEEAYQLLLENCQLIENDLKTHIYDAIVEQNSFYLGAEG
                     ASPQVAQNSDKLKALCLTKEEWRKAYQFLFIKAAQTEQLQANHQFTPDAIGFILLYLL
                     EQLSDKDSLEVLEIGSGTGNLAQTLLNNTSKSLDYVGIELDDLLIDLSASIAEIMDSS
                     AHFIQEDAVRPQLLKESDIVISDLPVGYYPNDDIAKRYKVASSDKHTYAHHLLMEQSL
                     KYLKKDGFAIFLAPVNLLTSPQSQLLKQWLKDYAQVVTLITLPDSIFGHPSNAKSIIV
                     LQKQTDHPMETFVYPIRDLKLAENIHDFMENFKKWKLSNVN"
     gene            21338..22534
                     /locus_tag="VC122_000527"
     CDS             21338..22534
                     /locus_tag="VC122_000527"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014407217.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetate kinase"
                     /protein_id="HEP5640473.1"
                     /translation="MSKTIAINAGSSSLKWQLYQMPEEEVLAQGIIERIGLKDSISTV
                     KYDGKKEEQILDIHDHTEAVKILLNDLIHFGIIAAYDEITGVGHRVVAGGELFKESVV
                     VNDKVLEQIEELSVLAPLHNPGAAAGIRAFRDILPDITSVCVFDTSFHTSMAKHTYLY
                     PIPQKYYTDYKVRKYGAHGTSHKYVAQEAAKMLGRPLEELKLITAHIGNGVSITANYH
                     GKSVDTSMGFTPLAGPMMGTRSGDIDPAIIPYLIEQDPELKDAADVVNMLNKKSGLSG
                     VSGISSDMRDIEAGLQEDNPDAVLAYNIFIDRIKKCIGQYFAVLNGADALVFTAGMGE
                     NAPLMRQDVIGGLTWFGMDIDPEKNVFGYRGEISTPESKVKVLVISTDEELCIARDVE
                     RLKNTK"
     gene            22721..23029
                     /locus_tag="VC122_000528"
     CDS             22721..23029
                     /locus_tag="VC122_000528"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011528165.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEP5640474.1"
                     /translation="MYVFIEPNDLKDYLITVYDERNRAIRKQTQVYCFYLMIVLFVAL
                     FFFFPCLTIRDLVSYKLALIFAYLYLIIRSLVAKKMEALGKITTNSQKPPFDTSQVLC
                     "
     gene            complement(23112..23882)
                     /locus_tag="VC122_000529"
     CDS             complement(23112..23882)
                     /locus_tag="VC122_000529"
                     /EC_number="1.5.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009880354.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyrroline-5-carboxylate reductase"
                     /protein_id="HEP5640475.1"
                     /translation="MKIGIIGVGKMASAIIKGLKQTPHELIISGSSLERSKEIAEQLT
                     LPYAMSHQDLIDQVDLVILGIKPQLFETVLKPLHFKQPIISMAAGISLQRLATFVGQD
                     LPLLRIMPNMNAQILQSSTALTGNALVSQELQARVRNLTDSFGSTFDISEKDFDTFTA
                     LAGSSPAYIYLFIEALAKAGVKNGMPKAKALEIVTQTVLASASNLKTSSQSPHDFIDA
                     ICSPGGTTIAGLMELERLGLTTTVSSAIDKTIDKAKSL"
     gene            complement(23930..24997)
                     /gene="pepA"
                     /locus_tag="VC122_000530"
     CDS             complement(23930..24997)
                     /gene="pepA"
                     /locus_tag="VC122_000530"
                     /EC_number="3.4.11.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012766453.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamyl aminopeptidase"
                     /protein_id="HEP5640476.1"
                     /translation="MTDLFSKIKEVTELDGIAGYEHSVRDYLRTKITPLVDRVETDGL
                     GGIFGIRDSKAEKAPRILVAAHMDEVGFMVSDIKVDGTLRVVGIGGWNPLVVSSQRFT
                     LYTRTGQVIPLISGSVPPHFLRGANGSASLPNIENIVFDGGFTDKAEAESFGITPGDI
                     IVPQSETILTANQKNIISKAWDNRYGVLMITEMLEALKGQDLNNTLIAGANVQEEVGL
                     RGAHVSTTKFDPELFFAVDCSPAGDIYGNPGTIGDGTLLRFYDPGHVMLKDMRDFLLT
                     TAEDAGVNFQYYCGKGGTDAGAAHLQNGGVPSTTIGVCARYIHSHQTLYAMDDFVEAQ
                     AFLQAIIKKLDRSTVDLIKRY"
     gene            25453..25746
                     /locus_tag="VC122_000531"
     CDS             25453..25746
                     /locus_tag="VC122_000531"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002986518.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4651 domain-containing protein"
                     /protein_id="HEP5640477.1"
                     /translation="MRDMSKKKIGMISGIFGFSLAIGLGIVIKDYCQDRQRRQMTRDL
                     RTFFSPLGQIEVLYINPCQVKQDYISGGVVMSNGKQYQFTYHSRQISFEEDKG"
     gene            25743..26060
                     /locus_tag="VC122_000532"
     CDS             25743..26060
                     /locus_tag="VC122_000532"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002987805.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin family protein"
                     /protein_id="HEP5640478.1"
                     /translation="MIRPTSYESLAALIEKEDKLVLFFTADWCPDCQFIYPIMPEIEA
                     ELTDMTFICVNRDQFIEVAQKWNIFGIPSFVVIEKGQEVGRLVNKMRKTKTEIMHFLA
                     AYQ"
     gene            26078..26704
                     /locus_tag="VC122_000533"
     CDS             26078..26704
                     /locus_tag="VC122_000533"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011284429.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4479 and tRNA-binding domain-containing
                     protein"
                     /protein_id="HEP5640479.1"
                     /translation="MIFAYNKEQVGDVLMVILQDTKDIKRQVERKGKVARVFAEESGK
                     TLAWNIFEVSSLITIEGNGQIFLTDEDLARLNAELAKEGFSERLEPTVGPVFVVGQIV
                     EMVAHPDSDHLNICQVAIGEDQTVQIVAGAPNAALGLKTIVALPGAIMPNGSLIFPGK
                     LRGEESYGMMCSPRELVLPNAPQKRGIIELDESAVVGEAFDPAKHWKG"
     gene            26856..27251
                     /locus_tag="VC122_000534"
     CDS             26856..27251
                     /locus_tag="VC122_000534"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000282448.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="single-stranded DNA-binding protein"
                     /protein_id="HEP5640480.1"
                     /translation="MYNKVIAIGRLVAKPELVKTATDKHVARLSLAVNRRFKNASGER
                     EADFISIVVWGKLAETLVSYASKGSLMSIDGELRTRKYDKDGQVHYVTEVLCQSFQLL
                     ESRAQRAMRENNVTNDLVDLVLEEDTLPF"
     gene            complement(27512..28153)
                     /locus_tag="VC122_000535"
     CDS             complement(27512..28153)
                     /locus_tag="VC122_000535"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014635233.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="deoxynucleoside kinase"
                     /protein_id="HEP5640481.1"
                     /translation="MLIVLAGTIGAGKSSLAAALGEHLGTDVFYEAVDNNPVLDLYYQ
                     DPKKYAFLLQIYFLNKRFKSIKEAYQADNNILDRSIFEDELFLKLNYKNGNVTKTELD
                     IYQELLANMLEELEGMPKKRPDLLIYIDVSFDKMLERIEKRGRSFEQVDGNPSLEQYY
                     HQVHGEYPTWYEDYKVSPKMKIDGNSLDFVQNPQDLATVLKMIDTKLKELHLL"
     gene            complement(28173..29150)
                     /gene="dusB"
                     /locus_tag="VC122_000536"
     CDS             complement(28173..29150)
                     /gene="dusB"
                     /locus_tag="VC122_000536"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011888540.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA dihydrouridine synthase DusB"
                     /protein_id="HEP5640482.1"
                     /translation="MTKLNSSFRIGDVEIPHRTVLAPMAGVTNSAFRTIAKEFGAGLV
                     VMEMISEKGLLYNNEKTLHMLHIDENEHPMSIQLFGGDAEGLKRAADFIQTNTKADIV
                     DINMGCPVNKVVKNEAGAKWLRDPDKIYHIVKEVTSVLDIPLTVKMRTGWADSSLAVE
                     NALAAESAGVSALAMHGRTREQMYTGTCDHETLARVSKAITKIPFIGNGDVRSVQDAK
                     FMIEEIGVDAVMIGRAAMNNPYLFTQINHFFETGQELPDLPFAKKLDIAKDHLKRLIN
                     LKGETIAVREFRGLAPHYLRGTAGAAKVRGAVSRAETLAEVEAIFKTVR"
     gene            complement(29137..30009)
                     /gene="hslO"
                     /locus_tag="VC122_000537"
     CDS             complement(29137..30009)
                     /gene="hslO"
                     /locus_tag="VC122_000537"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002994071.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Hsp33 family molecular chaperone HslO"
                     /protein_id="HEP5640483.1"
                     /translation="MDKIIKSIAQSGAFRAYVLDSTETVALAQEKHNTLSSSTVALGR
                     TLIANQILAANQKGDSKITVKVIGDSSFGHIISVADTKGHVKGYIQNTGVDIKKTATG
                     EVLVGPFMGNGHFVTIIDYGTGNPYTSTTPLITGEIGEDFAYYLTESEQTPSAIGLNV
                     LLDENDKVKVAGGFMVQVLPGASEEEIARYEKRLQEMPAISHLLASKNHVDALLEAIY
                     GDEPYKRLSEEPLSFQCDCSRERFEAALMTLPKADLQAMIDEDKGAEIVCQFCGTKYQ
                     FNENDLEALINDKA"
     gene            complement(30156..31649)
                     /gene="rofA"
                     /locus_tag="VC122_000538"
     CDS             complement(30156..31649)
                     /gene="rofA"
                     /locus_tag="VC122_000538"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_047149466.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator RofA"
                     /protein_id="HEP5640484.1"
                     /translation="MIEKYLESSIESKCQLIILFFKTSYLPITEVAEKTGLTFLQLNH
                     YCEELNAFFPGSLSMTIQKRMISCQFTHPFKETYLYQLYASSNVLQLLAFLIKNGSHS
                     RPLTDFARSHFLSNSSAYRMREALIPLLRNFELKLSKNKIVGEEYRIRYLIALLYSKF
                     GIKVYDLTQQDKNTIHSFLSHSSTHLKTSPWLSESFSFYDILLALSWKRHQFSVTIPQ
                     TRIFQQLKKLFVYDSLKKSSHDIIETYCQLNFSAGDLDYLYLIYITANNSFASLQWTP
                     EHIRQCCQLFEENDTFRLLLNPIITLLPNLKEQKASLVKALMFFSKSFLFNLQHFIPE
                     TNLFVSPYYKGNQKLYTSLKLIVEEWMAKLPGKRDLNHKHFHLFCHYIEQSLRNIQPP
                     LVVVFVASNFINAHLLTDSFPRYFSDKSIDFHSYYLLQDNVYQIPDLKPDLVITHSQL
                     IPFVHHELTKGIAVAEISFDESILSIQELMYQVKEEKFQADLTKQLT"
     gene            31916..33586
                     /gene="sfbI"
                     /locus_tag="VC122_000539"
     CDS             31916..33586
                     /gene="sfbI"
                     /locus_tag="VC122_000539"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014407228.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fibronectin-binding protein SfbI"
                     /protein_id="HEP5640485.1"
                     /translation="MNNKIFLNKEAGFLAHTKRKRRFAVTLVGVFFMLLACAGAIGFG
                     QVAYAADEKTVPNFKSPDPDYPWYGYDAYTGAFLRYHDLRVNLNGSRSYQVYCFNIKK
                     NYPRPFTSSDKKWYKRLEGTAETFKVHAMAPRVGGEELTKKLRSVMYNGYPNDGNNIM
                     KGLEPSNAIEVTQYAVWYYSDKSQYEIETLWKSEAKEGKISESQVTLMREALRKLISS
                     DEDLVKQVPSNFKLSTFESSDKSYQNLLSAEFVPDDPPKPGDTSEHNPKTPELDGSPI
                     PEDPKHPDDNLEPTLPPVMLDGEEVPEVPSESLEPALPPLMPELDGEEVPEVPSESLE
                     PALPPLMPELDRQEVPEKPSVDLPIEVPRYEFNNKDQSPLAGESGETEYITEVYGNQQ
                     NPVDIDKKLPNETGFSGNMVETEDTKEPEVLMGGQSESVEFTKDTQTGMSGQTTPQVE
                     TEDTKEPGVLMGGQSESVEFTKDTQTGMSGFSETVTIVEDTRPKLVFHFDNNEPKVEE
                     NREKPTKNITPILPATGDIGNVLAFLGILILSVLSIFSLLKNKQNNKV"
     gene            33874..34722
                     /locus_tag="VC122_000540"
     CDS             33874..34722
                     /locus_tag="VC122_000540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014407229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class C sortase"
                     /protein_id="HEP5640486.1"
                     /translation="MTERLKNLGILLLFLLGTAIFLYPTLSSQWNAYRDRQLLSTYHK
                     QVTQKKPSEMEEVWQKAKAYNARLGIQPVPDAFSFRDGIHDKNYESLLQIENNDIMGY
                     VEVPSIKVTLPIYHYTTDEVLTKGAGHLVGSALPVGGDGTHTVISAHRGLPSAEMFTN
                     LNLVKRGDTFYFRVLNKVLAYKVDQILTVEPDQVTSLSGVMGKDYATLVTCTPYGVNT
                     KRLLVRGHRIAYHYKKYQQAKKAMKLVDKSRMWTEVVCAAFGVVIAIILVFMYSKMTD
                     KKVIKN"
     gene            35008..37251
                     /locus_tag="VC122_000541"
     CDS             35008..37251
                     /locus_tag="VC122_000541"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011284436.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pilus ancillary protein 1"
                     /protein_id="HEP5640487.1"
                     /translation="MNNKKLQKKQDAPRVSNRKPKQLTVTLVGVFLMLLVLIGFEGKV
                     RAAHELVEVPVPIFHNPDPQSDYQWYGYEAYTGGYPKYDLFKTYYHDLRVNLHGSKSY
                     QVYCFNVHKHYPRSSQSFDRKWYKKLDGTAENFDSLAMEPRVRKEELTKKLRAVMYNA
                     YPNDANGIMKDLEPLNAIKVTQEAVWYYSDSAQINPDESFKTEAQSNGINDQQLGLMR
                     KALKELIDPNLGSKYSNKTPSGYRLNVFESHDKTFQNLLSAEYVPDTPPKPGEEPPAK
                     TEKTSVIIRKYAEGDYSKLLEGATLRLTGEDIPDFQEKVFQSNGTGEKIELSNGTYTL
                     TETSSPDGYKIAEPIKFRVVNKKVFIVQKDGSQVENPNKEVGSPYTIEAYNDFDEFGL
                     LSTQNYAKFYYGKNYDGSSQIVYCFNANLKSPPDSEDHGATINPDFTTGDIRYSHIAG
                     SDLIKYANTARDEDPQLFLKHVKKVIENGYHKKGQAIPYNGLTEAQFRAATQLAIYYF
                     TDSVDLTKDRLKDFHGFGDMNDQTLGVAKKIVEYALSDEDSKLTNLDFFVPNNSKYQS
                     LIGTEYHPDDLVDVIRMEDKKQEVIPVIHSLTVKKTVVGELGDKTKGFQFELELKDKT
                     GQPIVDALKTNNQDLVAKDGKYSFNLKHGDTIRIEGLPTGYSYTLKETEAKDYIVTVD
                     NKVSQEAQSASENVTADKEVTFENRKDLVPPTGFTTDGGTYLWLLLLVPFGLLVWFFG
                     RKGLKND"
     gene            37244..37765
                     /gene="lepB"
                     /locus_tag="VC122_000542"
     CDS             37244..37765
                     /gene="lepB"
                     /locus_tag="VC122_000542"
                     /EC_number="3.4.21.89"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002987832.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="signal peptidase I"
                     /protein_id="HEP5640488.1"
                     /translation="MTNYLNRLNENPLFKAFIRLVLKISIIGFLGYILFQYVFGVMIV
                     NTNQMSPAVSAGDGVLYYRLTDRYHINDVVVYEVDNTLKVGRIAAQAGDEVNFTQEGG
                     LLINGHPPEKEVPYLTYPHSSGPNFPYKVPTGTYFILNDYREERLDSRYYGALPINQI
                     KGKISTLLRVRGI"
     gene            37787..38824
                     /locus_tag="VC122_000543"
     CDS             37787..38824
                     /locus_tag="VC122_000543"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011017261.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="QVPTGV class sortase B protein-sorting
                     domain-containing protein"
                     /protein_id="HEP5640489.1"
                     /translation="MKKNKLLLATAILATALGTASLNQNVKSETAGVSENAKLIVKKT
                     FDSYTDNEVLMPKADYTFKVEADSSATDKTKDGLEIKPGVTEGLTTEQTIAYDNSVKP
                     SDKSKTATFDFSTVKFPEVGVYRYTVSEIDSKVSGIKYDTKTWIVDVYVVNDGNGGFK
                     ARYIVSKEKGQNDKKPVVFENSFKTTSLKVEKQVTGNTGELKKDFNFTLTINPNDNFV
                     AGQVIKLEKGGIKADVKIGEPYKFALKNGEKVTLSKLPVGVTYSIIEDEADKDGYTTN
                     AKITDGTAAPVEYKLGNQQLADESADEIVVTNNRDTQVPTGVVGTLAPFAVLSIVAIG
                     GVIYITKRKKA"
     gene            38840..39565
                     /gene="srtB"
                     /locus_tag="VC122_000544"
     CDS             38840..39565
                     /gene="srtB"
                     /locus_tag="VC122_000544"
                     /EC_number="3.4.22.71"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002995430.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class B sortase"
                     /protein_id="HEP5640490.1"
                     /translation="MMMTIVQVINKAIDTLILIFCLVVLFLAGFGLWDSYHLYQQADA
                     SNFKKFKTAQQQPKFEDLLALNEDVIGWLNIPGTHIDYPLVQGKTNLEYINKAVDGSV
                     AMSGSLFLDTRNHNNFTDDYSLIYGHHMAGNAMFGEIPKFLKKDFFNKHNKAIIETKE
                     RKKLTVTIFACLKTDAFDQLVFNPNAITNQDQQRQLVDYISKRSKQFKPVKLKHHTKF
                     VAFSTCENFSTDNRVIVVGTIQE"
     gene            39582..40169
                     /locus_tag="VC122_000545"
     CDS             39582..40169
                     /locus_tag="VC122_000545"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011017263.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial protein"
                     /protein_id="HEP5640491.1"
                     /translation="MRKYWKMLFSVVMILTMLAFNQTVLAKDSTVQTSISVENVLERA
                     GDSTPFSIALESIDAMKTIEEITIAGSGKASFSPLTFTTVGQYTYRVYQKPSQNKDYQ
                     ADTTVFDVLVYVTYDEDETLAAKVISRRAGDEEKSAITFKPKRLVKPIPPRQPDIPKT
                     PLPLAGEVKSLLGILSIVLLGLLVLLYVKKLKSRL"
     gene            complement(40328..41533)
                     /locus_tag="VC122_000546"
     CDS             complement(40328..41533)
                     /locus_tag="VC122_000546"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012560395.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YSIRK-targeted surface antigen transcriptional
                     regulator"
                     /protein_id="HEP5640492.1"
                     /translation="MVIFDLKYVQTLHSLSQLPISVMSQDKALIQVYGNDDYLLCYYQ
                     FLKHLAIPQAAQDVIFYEGLFEESFMIFPLCHYIIAIGPFYPYSLNKDYQEQLANNFL
                     KHSSHRSKEELLSYMALVPHFPINNVRNLLIAIDAFFDTQFETTCQQTIHQLLQHSKQ
                     MTADPDIIHRLKHISKASSQLPPVLEHLNHIMDLVKLGNPQLLKQEINRIPLSSITSS
                     SISALRAEKNLTVIYLTRLLEFSFVENTDVAKHYSLVKYYMALNEEASDLLKVLRIRC
                     AAIIHFSESLTNKSISDKRQMYNSVLHYVDSHLYSKLKVSDIAKRLYVSESHLRSVFK
                     KYSNVSLQHYILSTKIKEAQLLLKRGIPVGEVAKSLYFYDTTHFHKIFKKYTGISSKD
                     YLAKYRDNI"
     gene            41918..45412
                     /locus_tag="VC122_000547"
     CDS             41918..45412
                     /locus_tag="VC122_000547"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002987847.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fibronectin binding protein"
                     /protein_id="HEP5640493.1"
                     /translation="MTQKNSYKLSFLLSLTGFILGLLLVFIGLSGVSVGHAETRNGAN
                     KQGSFEIKKVDQNNKPLSGATFSLTPKDGKGKPVQTFTSSEEGIIDAQNLQPGTYTLK
                     EDKAPDGYDKTSRTWTVTVYENGYTKLVENPYNGEIISKAGSKDVSSSLQLEKSTMSV
                     FSKYGGTEVTSGAADFYRNHAAYFKMSFELKQKDKSETINPGDTFVLQLDKRLNPKGI
                     SQDIPKIIYDSENSPLAIGKYDAKTHQLTYTFTNYIAGLDKVQLSAELSLFLENKVVL
                     ENQTISDFKSTIGDQEIKYGGTVNVLYGNASGQDNNYVTNGLSNVGGSIESYNTQTGD
                     FTWYVYVNPNRTNIPYATMNLWGFGRLVKSNNSTSKSEDSATSSAQLEKVEIYEVKED
                     YTLPTSYGVDLTKLNLRKDLEAKLPQDSTQNSNKRLRIDFGDKLQNKAFVVKITGKTD
                     QSGKPLVVQSNLASFNNETAVTYGNPVNNVYFQNEIALSPSKGSGSGTSEFTKPSITV
                     ANLKRVAQLRFKKMSTDNVPLPEAAFELRSSNGNSQKVEAKSNTQGEVHFKDLTSGTY
                     DLYEKQAPKGYQQVTEKLATVTVDTTKPAEEMVTWGSPHSSVKVEDSKEVTIVNHKET
                     LTFSGKKIWENDRPDQRPAKIQVQLLQNGQKMSDKVQEVTKDNDWSYHFKDLPKYDDR
                     NQEYKYSVEEVKVPDGYKVSYLGNDIFNTRETEFVFEQNNFNLEFGNAEIKGQSGSKI
                     IDEDTLTSFKGKKIWKNDTAENRPQAIQVQLYADGVAVEGQTKFISGSGNEWSFEFKN
                     LKKYNGTGNDIIYSVREVTVPTGYDVTYSANDIINTKREVITQQGPKLEIEETLPLES
                     GASGGTTTVEDSRPVDTLSGLSSEQGQSGDMTIEEDSATHIKFSKRDIDGKELAGATM
                     ELRDSSGKTISTWISDGQVKDFYLMPGKYTFVETAAPDGYEIATAITFTVNEQGQVTV
                     NGKATKGDAHIVMVDAYKPTKGSGQVIDIEEKLPDEQGHSGSTTEIEDSKSSDLIIGG
                     QGEVVDTTEDTQSGMTGHSGSTTEIEDSKSSDVIIGGQGQVVETTEDTQTGMHGDSGC
                     KTEVEDTKLVQSFHFDNKESESNSEIPKKDKPKSNTSLPATGEKQHNMFFWMVTSCSL
                     ISSVFVISLKTKKRLSSC"
     gene            complement(45678..46343)
                     /locus_tag="VC122_000548"
                     /pseudo
     CDS             complement(45678..46343)
                     /locus_tag="VC122_000548"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011184105.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
BASE COUNT        14475 a         8114 c         9846 g        13914 t
ORIGIN      
        1 cgtaggttca aatcctgctc ccgcaataca taagtatgta gttgttggct cggtagctca
       61 gttggtagag caatggattg aagctccatg tgtcggcggt tcgattccgt ctcgcgccat
      121 taaaatttaa tattttacgg aaagatagcg aagaggctaa acgcggcgga ctgtaaatcc
      181 gctccttcgg gttcgggggt tcgaatccct ctctttccat cctaaacggg catagtttaa
      241 aggtagaact aaggtctcca aaaccttcag tgtgggttcg attcctactg cccgtgttag
      301 aaatatggcg ggtgtggtga agtggttaac acatcagatt gtggctctga cattcgtggg
      361 ttcgattccc atcactcgcc tattttattg gggtatagcc aagcggtaag gcaagggact
      421 ttgactccct catgcgttgg ttcgaatcca gctaccccag tttaaatttt atatttaaag
      481 ccggcgtggc ggaattggca gacgcgctgg actcaaaatc cagtgtccgc aaggacgtgc
      541 cggttcgacc ccggccgccg gtatacttga aaaacaaggt ttacaaacct tgttttttgt
      601 tttgatctta agctgtagct acgtagtaca atctcttata ttactgataa ggtctattta
      661 atgcgcaaga tttgtggtat gatgaatatg attgagtatt gatgctttat gggattaccg
      721 gtaaggtttt gaaaaaggtt tgtcactact aggtgctttt ttagctatag aaagacagtt
      781 atggattgat gtattgtaac taatgttgga ggttaatggt ggttgagttt tgagatattt
      841 acactgataa tcgggtggaa actggttggg tatcgggtaa tggagtgtgg ttcttcgttt
      901 aagcaaggag attatcgttt ggtggttcat atttgcttgt taaatgttag ggatgagatg
      961 ctgagtcagc aaggccatga agataaagct gactggccag agccttgaga tgtgacgttt
     1021 ggtggaagtc ctttttggtg agatggcaca gcatatagta atattagatc ctgaggaaga
     1081 attgggactt tcattaggct tgactggggt atgacctcac tttacaattc atttcgatga
     1141 tgggtttgat gatacgtttt tagttcagga ctttgtagag ttggagaatt tggttcttta
     1201 tgaggaagaa gtgcaggtaa ttcgttgaga cagtcgtgaa gatattttag gtatgattga
     1261 tgaaggtttg ttcattcctt atctgaagta taaaataaat ttgtgctttg agatggttgg
     1321 ccaatataaa gtccatcaat cttagaagta ataatttttg aattttttct gaggtttgtg
     1381 tatatttgat agtgtactga tgtaagaagt aaggagtact atgatggcaa tcaatgagag
     1441 agcttctgta aaaatcaagt taggccttga tactaagaat aagcgatgag atggttacca
     1501 cgatttatcg atggtcatga tgagtattga tctgtgtgac tatgtgacgg ttgaccatat
     1561 agatgacaat aagattgtct ttgcctcaaa ctgtccgaaa atacctatta actaagataa
     1621 tgatgtttat aagatagttc agctaatgaa acatcgattt caagttaaaa gaggagtttc
     1681 cgtttacttg gaaaagcgga ttcccatgtg tgctggtatg ggaggaggtt ctagcgatgc
     1741 tgttactatc agagcattaa atcagttatg gctccttact cttagccgaa aagatatgat
     1801 ggatattggt ataccgattg gtagtgatgt gccttattgc ttgctttcag gtcgtgccca
     1861 agtcactggt aaaggtgaag tggtttgtcg cattttgggg ctgttatcgt cttgggtggt
     1921 tttggttaaa ccagattttg gtatctcgac ttgaacattt tttctagata ttaattgtaa
     1981 aatcatttca agagtgtcta ctactcattt ggttgcggcg attgaagcgg gtaactataa
     2041 tgatgggata ttgactgaaa tgaacaattt gctggaagat attttcattg ccaagcgacc
     2101 ctttattcaa aaaataaaag agaaaacact gcaagctggt gctgctacta ctttgatgac
     2161 aggaagtgga ccgactgttt ttgctttgtg ccagaccgaa aaacaaacta taatagttta
     2221 aaaggacttt gcaaggaagt ttttaaggtg agaatgttgt aatattttgt tgggattgct
     2281 tctttggaag cttcatgtta taattaactg gttaatgaaa gaggagattt atggggactt
     2341 tagaaaaaaa acttgataac ttagtaaata ctattttatt aaaagcagaa aatcagcatg
     2401 agttattatt tggagcttgt caaagtgacg ttaagcttac taatacacaa gaacatattt
     2461 taatgttact atctcagcaa cgtctcacta atacagattt ggctaaggca ttaaatatta
     2521 gtcaggcggc agtaactaag gctatcaaga gtttggtcaa acaagacatg ctagcaggaa
     2581 ctaaggatac ggttgatgct agggtgactt attttgaatt aaccgagtta gctaagccta
     2641 ttgcgtcaga acatacccat catcatgatg aaaccttaaa tgtttacaac cgtttattac
     2701 aaaaattctc cgcgaaagaa ttagagattg tagataagtt tgtaacagtt tttgctgagg
     2761 aattagaagg gtaatccatg agatacatat cagtgaaaaa tctctccttt caatatgaaa
     2821 gtgagccagt tttagaaggg atcacttatc atttagatag tggagaattt gtcaccatga
     2881 ccggtgaaaa tggcgctgca aagtcaacct taataaaagc aaccttagga attttacaac
     2941 caaaggctgg acgagttact attgctaaaa aaaataaaga cggtaaacaa ttaaggattg
     3001 cttacttgcc gcagcaagta gctagcttta acgctggttt tccatccacc gtttacgagt
     3061 ttgtcaaatc aggtcgctac ccacgtagtg gttggtttag acatttgaac aaacacgatg
     3121 aagagcatgt gcaagcaagc ttagaagcag tcggcatgtg ggaaaaccgt cataagagaa
     3181 ttggtagttt atcaggtggt caaaaacaac gtgtggttat tgcccgtatg tttgcttctg
     3241 accctgatat ttttgtgcta gacgagccaa caacgggaat ggatagcggt actactgata
     3301 ccttttatga actgatgcac cacagtgcac atcaacacgg gaaatccgtt ctgatgatta
     3361 cccatgaccc agaagaagtg aaggcttatg ctgatcggaa cattcattta gtcagaaacc
     3421 aaaaacttcc ttggcgttgt ttcaacattc atgaagttga aacagatgac gaaaaaggag
     3481 gtcatggtca tgcttgatat tttatcctat gatttcatgc aacgggcggt aatggcggta
     3541 gttgccatta gtatttttgc tccgatttta ggtattttcc ttattttacg tcgtcaaagt
     3601 ttgatgagcg atacccttag tcatgtttct ttggctgggg tagcgcttgg ggtagtcctt
     3661 ggtatttcac caaccatcac tactattatt gttgtggttt tagctgctat tttgttagaa
     3721 tacctgcgtg tagtttacaa acactacatg gagatttcaa cggcgatttt gatgtcactt
     3781 ggcttggccc tatctctgat tattatgagt aagtcgcata gttcatcaag catgagttta
     3841 gaacaatacc tttttggatc gatcatcacg attagtatgg aacaagttgt cgccttgttt
     3901 gctattgctg cgattatttt aatcttgacc gttctcttca ttagaccgat gtacattctg
     3961 acctttgatg aagatactgc ttttgtagat ggtttgcccg ttcgcttgat gtctgttcta
     4021 ttcaatatcg tcactggggt tgctattgct ttgaccattc cagcagcagg agcacttttg
     4081 gtttctacca ttatggtctt gccagcaagt atcgcaatga gattgggtaa aaactttaaa
     4141 acagttatct tactgggaat tgtcatcggt tttagcggta tgttatctgg tattttctta
     4201 tcttatttct ttgaaacgcc agctagtgcc actattacca tgattttcat tagtattttc
     4261 ctcttagtta gtctaggtgg aatgcttaaa aaacggttat tttaataaga aaaatcctca
     4321 catgtttaac atgtgaggat ttatttataa atgctttaaa aagggggtaa ggtcccaatt
     4381 ttcttgatta atggtgatgc tctcccaaaa gctatcatta gttaaacgag gaatcaaatg
     4441 aaggtgaaaa tgagtcccct catccatcaa ctccttgtca ttgctgacgc tagtgacgcc
     4501 tgaaatggga aggtgctgct ctagcttagc aactagagcg gcttgtaagt cgatgagatc
     4561 atagcgcact tcctttggta gttgtgtcag actagtgtaa tggtctttgg aaatcagtaa
     4621 aaggtgcccc ttttggatag gatcaatatc aaaaacaacc ttgaaatatt gactttgagt
     4681 cagtagctga tttgctttga gctgatggca aaagatacat tctgtcatag gtacatctcc
     4741 ttatgaaaaa acgagccaga gctcgtttaa tcggttatct agtaagtgag aacagcgtat
     4801 tttttcttac cgcggcgaat aacggttaat tgatcatcaa ttttatcatc attacttaat
     4861 tggtaatcta aatcttgaac gcggtctccg ttaatgtaga ttgctccatt ttgcacgtct
     4921 tcacgcgctt ggcgttttga tggggaaata cctgcggtga caagcatgtc aacaaggtta
     4981 aggctgtctt ctgattgaac gtgataattt ggtacattgc tcaatccttg ttttaattca
     5041 ttagcagaaa gatttttgat gtttccagca aataactgct cggtaatgtt aagagcttgt
     5101 ttataagctt cttcgccatg aaccaaggta accacttcgc gtgccaaggt tttttgagcg
     5161 agacgctcgt gacgagcggc attaaattga gtttcaattt ctgcaatctc atctagaaat
     5221 aagaaggtga agattttcaa gaaacgcact gcatcatcat ccatgacatt taaccagaat
     5281 tggtacatct cgtaaggaga cgtcttatcg gcatcaagcc agacggcatt accttctgat
     5341 ttaccgaatt ttttcccggt tgagtcagtg atgagtggta cagtcatgac gtgcccagtt
     5401 ttatcagcct ttttgcggag taattcagta ccagctgtca tattgcccca ctggtcagag
     5461 ccgccaattt gtaaggttac attatgcttg tcattgagtt cgtagaagtc gtagccctgc
     5521 atgatttggt aagcaaactc agtgtaagaa atgcctgttt caatgcgttt tttaacagag
     5581 tctttactca tcatgtagtt aacggtgaag tatttaccga catcacggag gaagtcaata
     5641 aagctgattt gcgagaacca gtcatagttg ttgacaagct ctgctttatt atcaccattt
     5701 tcaaaatcaa ggaaagtaga caactgccct ttaatcttgt cactccactc caaaactgtt
     5761 tctttggttt gaaggctgcg ctctgcatct ttaaaggaag gatcaccaat taaacctgtg
     5821 gcaccaccca caagagcgta aggtttgtgc cctgctagtt gcaagcgacg agatgttaag
     5881 atagccacta gatggcccag atgaaggctg tcagcggttg gatcataacc ggtataatag
     5941 gatacttgcc cttctgttaa tgctttgaca agggcttgtt catcagtcgt ttgaaagacc
     6001 aagccacgag ctttgagttc ttcaaaaata ttcataggct ttctccattt tcattatagt
     6061 ttcgaatcat tatatcaaaa aacgctactg ctatgcaagc tttatcaaag tttggtataa
     6121 tgataaatga ggtattttat ggtgaaatgg aacacgaaac aaaagcgtat aagtcatcaa
     6181 agattaggtc tcttggattt ggggccggtg ctattacgta cgttgagact actgtctaac
     6241 tttttttata ttgttatctt cctttttgga atgatgggat ttggtatggc atttgggtat
     6301 ttggctagtc agattgaatc tgttaaggta ccaagtaaag aaagtttagt caaacaagtt
     6361 gaatcattaa cgatgatttc gcaaatgaac tattctgata atagtttaat ttctacttta
     6421 gatacggatt tacttcgaac accagtagct aatgatgcga tttcagagaa tatcaaaaaa
     6481 gctattgtat caacagaaga cgaacatttt caagaacata aaggtgtcgt gccaaaagct
     6541 gtttttcggg caacattggc ttctgtattg ggatttggag aagctagtgg aggttcgacc
     6601 ttaacacagc aattggtcaa acaacaagtt ttgggagacg atcccacatt taagcgcaag
     6661 tctaaggaga tcgtttacgc tcttgcctta gagcgttata tgtccaaaga caatatctta
     6721 tgtgattatc ttaatgtttc accttttggt cgtaataaca agggccaaaa tattgctggt
     6781 gttgaagaag ctgcgcgtgg catttttggc gtttctgcca aagatttaac ggtgccacag
     6841 gcagcatttt tggcggggct tccgcagagt cctattgttt actctcctta tttgtcaacg
     6901 ggacaactga agtcagaaaa ggacatggct tatggcatca agcgtcagca aaatgttctc
     6961 tataacatgt accgtacagg tgttctgtct aaaaaagaat acgaggacta taaggcttat
     7021 ccgattcaaa aggattttat tcaaccggga agcgcaatag taaataatca cgattacctt
     7081 tattacacgg tgttagcgga tgctaaaaaa gccatgtata gctatttgat taagcgagat
     7141 aaggtgtcta gtcgtgactt gaaaaatgac gagactaagg ctgcttatga agagagagcc
     7201 ttaacagaat tgcaacaggg tggctatacc atcaccacaa ccattaataa gcctatttac
     7261 aatgcgatgc agacagcggc agctcagttt ggtggcctgt tagatgatgg cactggtaca
     7321 gttcaaatgg gaaatgtctt gacagacaat gcgactggtg ctgtgttagg ttttgttggt
     7381 ggtagagatt atgctctgaa tcaaaataat catgctttca atacaattag atcgccaggt
     7441 tctagcatta aaccgataat cgcttatggt cctgctattg atcaaggttt aatggggagt
     7501 gctagcgttt tgtctaatta cccaacaact tactcgagtg gccaaaaaat catgcatgct
     7561 gatagtgaag gaacagccat gatgccactt caagaggccc taaatacttc ttggaacatc
     7621 ccagcttttt ggacacagaa attactgcgt gaaaaaggtg tcgatgtcga aaattatatg
     7681 acaaaaatgg gttataagat tgcagactac tcgattgaaa gtttacctct agggggcggt
     7741 attgaagtct cggttgctca acaaaccaat gcttaccaaa tgctttcaaa caatggctta
     7801 tatcaaaagc aatatattgt agataagatc actgctagcg atggtacagt cgtttacaaa
     7861 catgaaaata agccaattcg tattttttct gcagcaacag ctacgatttt acaagaattg
     7921 ttgagaggtc cgattacttc aggcgctacg actactttca agaaccgttt ggcggctatt
     7981 aatccgtggc ttgctaatgc tgattggatt ggtaagaccg gaacaactga gaattatacg
     8041 gatgtttggc tagtcctgtc tactccaaaa gttaccttag gcggttgggc aggacatgat
     8101 gacaatacct cattagcgcc attaacagga tataacaata attctaatta ccttgcctat
     8161 ttagctaatg ccattaatca ggccgattcc aatgttattg gagtagggca acgtttcaac
     8221 ttagatccag gagtcattaa ggcgaatgtc ttgaagtcaa caggtttaca accaggaact
     8281 gttaatgtca atggacatac tttttctgtt ggtggagaaa tgaccaccag tctatggtcc
     8341 caaaaaggac cgggggctat gacttaccga tttgctattg gtggcacgga tgccgattat
     8401 caaaaagctt gggggaactt tgggttcaga aaaaattagg ttgccattta tcgataaaaa
     8461 gggtataata gcaataacta tttgtcgtcc aatctagctg aaatattgtc cagttaggaa
     8521 gaacagcagt taaatcacac tgataaagtc agatttagct gctctttttg tgtctatttt
     8581 tagaaaagat agggtttgta acctatattt aaatattcta aaaattaaca tttagggcaa
     8641 atgataactt agttgcgatt tgctgaaaca gcaaagctta aatagaagaa ggagctaaaa
     8701 acttggcagg acatgaagtt cgatacggaa aacaccgtac acgtcgtagc ttttcaagaa
     8761 tcaaagaagt tcttgattta ccaaatttga ttgaaattca aactgactca ttccaagatt
     8821 tcctcgattc aggtttgaaa gaagtatttg aagatgtact tcctatttca aactttacgg
     8881 atactatgga acttgaattt gttggttacg aatttaaaga acctaaatat acccttgaag
     8941 aagctcgtat ccacgatgca agttattctg caccaatctt tgttaccttc cgtttggtca
     9001 ataaagaaac tggtgagatt aaaactcaag aagtcttctt tggtgatttc ccaatcatga
     9061 cggaaatggg tacctttatt atcaatggtg gtgaacgtat catcgtttct cagttggtgc
     9121 gttctccagg tgtttatttc aacgataaag ttgataaaaa cggtaaagtt ggttacggat
     9181 caacagtgat tcctaaccgt ggggcttggt tggaattaga gactgactca aaagacattg
     9241 cctacactcg tattgaccgt acgcgtaaga ttccattcac aaccttggtt cgtgcccttg
     9301 gtttctcagg tgatgatgaa attgttgata tctttggtga aagcgacctt gttcgtaata
     9361 ccattgaaaa agacattcac aaaaatccaa gtgattctcg tacagacgaa gcccttaaag
     9421 aaatttacga gcgtcttcgt ccaggtgagc caaaaactgc agatagctct cgtagtctct
     9481 tgattgcgcg tttctttgat gcacgtcgtt acgatttggc tgcggttggt cgttacaaag
     9541 tgaacaaaaa acttaatatc aagactcgtc ttttgaacca aatcattgct gaaaaccttg
     9601 tggatgctga aactggcgaa attttggtag aagctggaac tgagatgact cgtagcgtca
     9661 tcgaatccat cgaagaacac cttgacggtg atttgaacaa gtttgtttac acaccaaatg
     9721 attacgctgt ggttactgaa cctgttgttc ttcaaaagtt caaggttgtg tcaccaattg
     9781 atcctgatcg tgtagtaact attgttggta atgccaatcc agatgataag gttcgtgcat
     9841 tgacacctgc tgatattttg gcagaaatgt catacttctt gaaccttgct gaaggtcttg
     9901 gaaaagttga cgatattgac cacttaggta accgtcgtat ccgtgccgtt ggtgaattgc
     9961 ttgccaacca attccgcatc ggtcttgctc gtatggagcg taacgttcgt gagcgtatgt
    10021 ctgttcaaga caacgatgtg ttaacaccac aacaaatcat caatatccgt cctgtcacag
    10081 cagctgtcaa agaattcttc ggttcgtctc agttgtcaca gttcatggac caacacaacc
    10141 cattgtcaga gttgtctcac aaacgtcgtt tatctgcctt aggacctggt ggtttgacac
    10201 gtgaccgtgc tggttatgag gttcgtgacg tgcattacac gcactatggc cgtatgtgtc
    10261 cgattgaaac acctgaagga ccaaatattg gtttgattaa caacttgtct tcatttggac
    10321 atcttaataa atatggtttc attcaaacac cttaccgtaa ggttgaccgt gcgactggta
    10381 gggtaactaa cgagattgtt tggttgactg ccgacgaaga agacgaatac acagttgcac
    10441 aggccaattc gaaactaaac gaagatggca cttttgctga agaaatcgtt atgggtcgtc
    10501 accaaggtaa taaccaagaa ttttctgcaa gtgttgttga tttcgttgac gtttccccta
    10561 aacaggtagt tgctgttgcg acagcatgta ttcctttctt ggaaaatgat gactccaacc
    10621 gtgctctcat gggtgccaac atgcaacgtc aggctgtgcc attgattgat ccaaaagcac
    10681 catatgttgg tactggtatg gaatatcaag ctgcccatga ctcaggcgct gcggtgattg
    10741 ctcagcacaa tggtaaagtt gtcttttctg atgctgaaaa agtggaaatc cgtcgtcaag
    10801 atggctcact tgatgtttac cacattacca aattccgtcg ttcaaactca ggaacagcct
    10861 ataaccaacg cacccttgtt aaagtaggag acattgttga aaaaggtgat ttcatcgctg
    10921 atggaccttc tatggaaaat ggtgaaatgg ctcttggaca aaacccagtc gttgcttaca
    10981 tgacttggga aggttataac tttgaggacg ctgttatcat gagtgagcgc cttgtgaaag
    11041 aagatgtcta cacatctgtt cacttggaag aattcgaatc tgaaacgcgt gatacaaaac
    11101 ttggccctga agaaatcact cgggaaatcc caaatgttgg tgaagaagcc ctcaaagacc
    11161 ttgacgaaat gggaattatc cgtatcggtg ctgaggttaa agaaggcgac atcttagtag
    11221 gtaaagtcac acctaaaggt gaaaaagacc tctctgctga agaacgttta cttcacgcca
    11281 tctttgggga taaatcgcgt gaagtgcgtg atacctcact tcgtgtccct cacggtggtg
    11341 atggtatcgt tcgtgatgtg aaaatcttta cacgcgctaa cggcgatgaa ttgcaatcag
    11401 gtgttaatat gcttgtgcgt gtttacatcg ctcaaaaacg taaaatcaag gtcggagata
    11461 aaatggccgg tcgtcacgga aacaagggtg tcgtttcacg tattgtacca gttgaagaca
    11521 tgccatacct tccagacgga acaccagttg acatcatgtt gaaccctctt ggggtgccat
    11581 cacggatgaa tattggtcag gttatggaac ttcaccttgg tatggctgct cgtaatcttg
    11641 gcattcacat tgcaacacct gtctttgacg gggcttcatc agaagacctt tgggacactg
    11701 ttcgtgaagc tggtatggat agcgatgcta agacggtcct ttatgatggt cgcaccggtg
    11761 aaccatttga caaccgtgta tccgttggtg tcatgtatat gatcaaactt caccacatgg
    11821 ttgatgataa acttcatgcc cgttctgtag gaccatactc acttgttacc caacagccac
    11881 ttggtggtaa agctcaattt ggtggacaac gttttggtga gatggaggtt tgggcccttg
    11941 aagcttatgg tgcatcaaat gttcttcaag aaatcttgac ctacaagtca gatgacgtca
    12001 caggacgttt gaaagcctat gaagcgatta ctaaaggtaa accaattcca aaaccaggtg
    12061 taccagaatc cttccgtgtt cttgtaaaag aattgcaatc gcttggtctt gatatgcgtg
    12121 tgcttgacga ggatgataat gaagtggaac ttcgtgatct tgatgaaggt gaagacgatg
    12181 acattatgca tgttgacgat ctcgagaagg cacgtgaaaa acaagctcaa gaaacccaag
    12241 aagtttctga aacaactgac gaaaaataag caatcaattc ttattaaata attatttact
    12301 ggtctggggc aaaggcccca ggaactggta aagtcatcaa aggcagaaag gtaaaactag
    12361 tggttgacgt aaatcgtttt aaaagtatgc aaatcacatt agcctcacca agtaaggtcc
    12421 gttcatggtc ttatggtgaa gttaaaaaac ctgaaacaat caactaccgt acattaaaac
    12481 cagaacgtga aggactcttt gacgaagtca tctttggtcc aacaaaagac tgggaatgtg
    12541 cgtgtggtaa gtacaaacgt atccgttata aagggatcgt ttgtgaccgc tgtggggttg
    12601 aggtaactcg tgctaaagta cgtcgtgaac gtatgggtca cattgagtta aaagctcctg
    12661 tatcacatat ctggtatttc aaagggattc catctcgtat gggattgact cttgatatga
    12721 gtcctcgtgc ccttgaagaa gtgatctatt ttgcggctta tgtggtcatt gatccaaaag
    12781 atacaccgct tgaaccaaaa tcattattaa cagaacgtga ataccgtgaa aaactacaag
    12841 agtatggtca tgggtcattt gtcgctaaga tgggtgctga agccattcaa gatctcttga
    12901 aacgtgtgga cttggcagct gaaatcgctg aattaaaaga agagttgaaa tctgcttctg
    12961 gtcaaaaacg gattaaagca gttcgtcgtt tagacgtcct tgatgccttt aacaaatctg
    13021 gaaataaacc agaatggatg gttcttaaca tcctgccggt tattccacca gatcttcgtc
    13081 cgatggttca attggatggt ggccgttttg cggcatcaga cttgaatgac ttgtaccgtc
    13141 gtgtgattaa ccgtaataac cgtttggcac gtttgttaga acttaatgcc cctggcatca
    13201 ttgttcaaaa tgaaaaacga atgcttcaag aagccgttga tgctttgatt gataatggtc
    13261 gtcgtggtcg tccaatcact ggaccaggaa gtcgtccatt gaaatcattg agccacatgc
    13321 ttaaaggtaa acaagggcgt ttccgtcaaa acttgcttgg taaacgtgta gacttctctg
    13381 gacgttccgt tatcgctgtt ggtccaaccc ttaaaatgta tcaatgtggt gtcccacgtg
    13441 aaatggctat cgagcttttt aaaccatttg taatgcgcga aattgttgcc aaagaatatg
    13501 ctggtaacgt taaagccgct aaacgtatgg ttgaacgtgg cgacgaacgc atctgggata
    13561 tcttagaaga agttatcaaa gaacacccag tcctacttaa ccgcgcaccg acccttcaca
    13621 gacttggtat tcaggctttt gaaccggttc ttattgacgg taaggcactt cgtcttcacc
    13681 cacttgtgtg tgaggcctac aatgccgact tcgatggaga ccaaatggcc atccacgtgc
    13741 cactttcaga agaagcacaa gctgaagctc gtcttttgat gcttgctgca gagcacatct
    13801 tgaaccctaa agatggtaaa ccagttgtta ctccatctca ggatatggtt cttggtaact
    13861 actatcttac gatggaagat gctggtcgtg aaggcgaagg tatgattttc aaggataaag
    13921 acgaagctgt gatggcatat cgtaatggtt atgctcatct tcatagtcgt gtgggtatcg
    13981 ctgttgacag catgccaaac aaaccttgga aagacagtca aagacataaa atcatggtga
    14041 caactgttgg taagattctc tttaacgata tcatgccaga ggaccttcct tacctccaag
    14101 agccaaacaa tgccaacttg acagaaggaa cacctgataa atacttcctt gaacctggtc
    14161 aagacatcca agaagtgatt gatcgcttag acatcaatgt gccatttaag aagaaaaacc
    14221 tcggtaacat cattgcggaa accttcaaac gtttccgtac aacagaaaca tcagccttcc
    14281 ttgaccgctt gaaagacctt ggttactacc attcaaccct tgccggtttg acagtgggta
    14341 tcgctgacat cccagttatc gataacaaag cagaaatcat tgatgctgct caccatcgtg
    14401 ttgaagaaat taacaaagcc ttccgtcgtg gtttgatgac agatgatgac cgttatgttg
    14461 ccgttacaac aacatggcgt gaagctaaag aagcccttga aaaacgtcta attgaaacac
    14521 aagatcctaa gaacccaatc gttatgatga tggactcagg agctcgtggt aacatctcta
    14581 acttctcaca gcttgctggt atgcgtggtt tgatggctgc tcctaacgga cgcatcatgg
    14641 aacttcctat cttgtcaaac ttccgtgaag gtttgagcgt tttggaaatg ttcttctcaa
    14701 cccacggtgc acgtaaaggg atgaccgata cggcccttaa aacagccgac tcaggttatc
    14761 ttactcgtcg tttggttgac gttgcccaag atgttatcat tcgtgaggac gattgtggca
    14821 ctgatcgtgg tcttcttatc cgtgctatta cagatggtaa agaagttacc gaaacgcttg
    14881 aagagcgtct tcaaggtcgt tacacacgta aatcagtcaa acaccctgaa actggtgaag
    14941 tcttgattgg tgctgaccaa ttaatcactg aagacatggc tcgtaagatt gttgatgcag
    15001 gtgttgaaga agtgaccatt cgttctgtct ttacctgtgc gactcgtcat ggtgtctgcc
    15061 gtcactgtta tggtatcaac ttggcaactg gtgatgctgt tgaagtgggt gaagcagttg
    15121 gtactattgc tgcccaatct atcggtgagc ctggtactca gcttaccatg cgtaccttcc
    15181 acacgggtgg tgtagcctca aataccgata tcacacaggg tcttcctcgt attcaagaga
    15241 tctttgaagc acgtaatcct aaaggggaag cggtcattac tgaagtgaaa gggaatgtcg
    15301 tcgagattga agaagatgcg tcaactcgta ccaagaaagt ctacgttcaa ggaaaaactg
    15361 gcatgggcga atatgtcgta ccatttacag cacgtatgaa agttgaagtt ggcgacgaag
    15421 ttaatcgtgg agctgccctt acagaagggt caattcaacc gaaacgtctc cttgaagtgc
    15481 gtgatacctt gtcagttgaa acgtaccttc ttgcagaagt acaaaaagtt taccgtagcc
    15541 aaggggtaga aatcggagac aaacacgttg aggtaatggt tcgccaaatg cttcgtaaag
    15601 ttcgtgtcat ggatccaggt gatacagacc tccttccagg tacacttatg gatatttctg
    15661 atttcacaga tgctaacaaa gatattgtta tctctggtgg tattcctgcg acatctcgtc
    15721 ctgttcttat gggtattact aaggcttccc ttgaaaccaa ttccttctta tcagctgcat
    15781 ccttccaaga aacaactcgt gttcttacag atgctgctat ccgtggtaag aaagaccact
    15841 tacttggtct taaagaaaat gttatcattg gtaaaatcat tccagctggt actggtatgg
    15901 cgcgctaccg taacattgaa ccacaagcga tgaatgagat tgaagtgatt gatcatacag
    15961 aagtctcagc agaagccgtt tttacagcag aagcagaata atctcatgaa agagtcctta
    16021 ggggctcttt tttgtctgag tttgttggca gtaaaaagca ttgacaatca gtcttagtcc
    16081 caatgagcag atgtttttca aaatacttat gatttagcta taataatgca taagaggaat
    16141 aggtaatgtg ttatgtatca agtaattaaa atgtatggtg attgggagcc ttggtggttc
    16201 atcgatggat ggcaagacga tattatagat gagcaacaat ttagtgactg gcaagaagcg
    16261 cttgattatt ttaatcaaga atggcagcgc atgaaagcta tttttcctag ttatcatagt
    16321 caaaagaatt tgttagctac tttttgggaa aaagaagata aaagatggtg cgaggactgt
    16381 gacgaagatt tacagcagtt tcattctctt ttactcctta aaaataaaga tattgtacca
    16441 agcaataatt atatccctga atttgaacaa cgaaatgatt caccacaggt agcttatctt
    16501 tgcaagttaa acctttaagt gtttgttaaa aacaaaataa gttaaaagaa aatatcactt
    16561 cttctgatat tttctttttt tgtttacgaa taaatgagta aggaggaatt atggtacaag
    16621 cattagcaaa agctattcta gcaaaagctg aacaggttca ggcacaagat atttatattt
    16681 tgccaagagc agatcaatat gatctttttt tacgaatagg agatgaaagg agattagttg
    16741 atgtttatca gagcgatcgg atggctcctc ttattagtca ctttaaattc gttgcaggaa
    16801 tgatagttgg tgaaaagaga cgttgtcagg tgggttcatg tgattataag cttagtaaag
    16861 ataagcagtt atctttgcgc ttatctagcg tgggtgatta tcgcgggcaa gaaagcttag
    16921 tgattcgtct gcttcatcat caaaataaaa gtgtacatta ttggtttgat ggattgacaa
    16981 aagtagccaa tcaggttggc ggtagagggt tgtatttatt tgcaggacca gttgggtctg
    17041 ggaagacaac cttgatgtac cagctgattt cgagttatca tcaagaagca caggttatta
    17101 gtatagaaga tcctgtagaa attaaaaatc accagatttt acaattacaa gtgaatgatg
    17161 atattggtat gacttatgac aatttgatca aactgtcttt acgccatcga ccagatattt
    17221 tagttattgg tgagattcga gatagtcaaa cagcaagagc cgttattagg gctagtctaa
    17281 caggtgccat ggttttttca acggttcacg ctaaaagtat ctcgggtgtt tatgtaagat
    17341 tgttagaact tggtgtaacg aaagcagaac tgtctaattg cttagcatta attgcttacc
    17401 aaaggttact taatggagga gcattgattg actctactca aaacgaattt gaatattatt
    17461 cctcatcgaa ctggaatcaa caaattgatc agcttcttga ggcaggacat ctcaatccca
    17521 agcaagctaa gcttgaaaaa attatctagt aaacatcagc ataaattcat tcaattacta
    17581 gctaatcttt tatctacagg atttagcttc gcagaggtta ttgctttttt aaaaagaagc
    17641 cagttgcttc agctagatta tgttcttaaa atggaagagt ctttattaaa aggacaagac
    17701 ttagcagata tgttgtcggg tttaggtttt tcagatgcta ttcttactca aattagttta
    17761 gctgatagac acggtaatat tgaaacaaca ttagtagcga ttcaacatta cttgaaccaa
    17821 atggcaagga tcagacgaaa aactgttgaa gttatcacct atcctcttat tcttttactt
    17881 tttctttttg tgatgatgct agggttgaga cgctatttag tccctcaatt agaaactcaa
    17941 aatcagataa cttactttct taaccatttt cctgccttct ttataggttt ttgctcaggt
    18001 cttattttgt tgttcgggat ggtatggcta cgatggcgat ctcaaagccg cttgaaactc
    18061 tatagtcggc taagccgtta tcctttttta ggtaaactgt taaaacaata cttaactagt
    18121 tattatgcta gagaatgggg tacgttaata ggtcaaggtc ttgatttgat gactatttta
    18181 gacattatgg ccattgagaa atcctcacta atgaaagaat tagctgagga catcagaatg
    18241 agtctgcttg aagggcaagc ctttcacatt aaagtagcta cctacccttt ttttaaaaaa
    18301 gaattaagtt taatgattga atacggtgaa atcaaatcga aactgggggc tgagttggaa
    18361 atctatgccc aggaaagctg ggaacaattt tttagtcagc tttatcaagt gacacaactt
    18421 attcaaccag ctattttttt agtagttgct gtgacaatcg tcatgattta tgcggcaatc
    18481 ctattaccaa tttaccaaaa tatgggaggt attttttaag atgattaatc aatggaacaa
    18541 cttacgacac aagaagctaa aaggatttac tcttctagaa atgttattgg tgattcttgt
    18601 catcagtgtt ttgatgctat tatttgtgcc taatttaagc aagcaaaaag acagggttac
    18661 agaaacaggt aatgccgctg ttgttaaatt agtggagaat caagcagaac tatatgaatt
    18721 atctcaaggc tcaaaaccaa gtttgagcca gttaaaggca gatggtagta tcactgagaa
    18781 acaagaaaaa gcttatcaag actattatga caaacataaa aatgaaaaag cccgtcttag
    18841 caattaaagc ttttactttg cttgaaactt tactaagttt gtcagtgatg agtttcatta
    18901 tattgggatt gtctgtacca gtgacaaaaa gctatcaaaa agtagaagag catctgtttt
    18961 ttagccattt tgaacatttg tatcgacatc agcaaaagtt agccatatta cagcaaaaac
    19021 agcgcgtctt ggatatttct tcaacaaaaa ttgttacaga aggtaatagc ctaacagttc
    19081 ctaaatcaat aacagtgaat catccttatc ggttagttat tgatcagatg ggaggcaacc
    19141 attccttagc taaaattatt tttgacatga ctgatcgacg ctttaaatac caattttatc
    19201 tagggagtgg gaattatcaa aaaacaagtc aaagcttaca tagcccttga aagtatgatc
    19261 gcaacaggca tcctctttag tattgtcatt ctcgtcttaa gcagtttaca gcagagtcag
    19321 gctgctctaa cgtactatcg aaagcagcaa gaaaagctta atctagcctt gatggcagta
    19381 caaactagaa ctaaagagat aacattgaat ggttgtcata ttactatttt acgtacggat
    19441 agatacatta gtattcacga tgacgaagga gaagtgatga agattgagta aacaattaag
    19501 taacataaaa gcttttaccc ttctagaggc gttaatagcc ttactcgtga tatcagggtc
    19561 tttattggtt tatcaaggtt tgacccaaac cctccttaaa cgtagccatt atctagcccg
    19621 tcatgatcaa gacaattggc tcttattttc tcatcaattg cgagaggagt taagtggagc
    19681 aagattttac aaagtagctg ataataaact atacgttgaa aaaggaaaga aagtactagc
    19741 ttttggccaa tttaaaagtc atgattttcg aaaatcagct agtaatggaa aagggtatca
    19801 acccatgtta tttggaatat cacgtagtca tattcacata gagcagtcac agatttgcat
    19861 tactttaaag tggaaaagtg ggttagaaag gactttttat tatgcctttc aagactaatc
    19921 tcaaagcagg catccttttg tatgctatgt ttatggctag tatttttttg ctggttttac
    19981 aggtttacct ttctcaagtg acagctctac acaaggaata ccaagctcaa actgactatg
    20041 ttaaagcccg actgatagca gaaatagttt atcaagatca tagatataaa gcttctaatc
    20101 ctgtcttttt taaaggtggt caagtcattt gtcgagagcg aaaagagaga tggatgttaa
    20161 ttgttaagtt agatcagcaa cgacagtatc agtttgaata ccttaaaccc aaacaatata
    20221 gtcaataaat agtattgttg tcataaatcc ataaaatgat ccaatatata gtaattaaag
    20281 gtgacacttc ttttcatttc aagtgtaatt tggtatgata aggttatgac ttttgaaaaa
    20341 attgaagaag cttatcagct gctcttagag aactgtcaac taattgaaaa tgatctaaaa
    20401 acacatatat acgatgccat tgttgaacaa aactcctttt atttaggggc tgagggagct
    20461 agtcctcaag ttgctcaaaa tagtgataaa ctgaaagcct tgtgcctgac aaaagaagaa
    20521 tggcgtaagg cctaccagtt tctttttatt aaggcagctc agacggagca actccaagcc
    20581 aaccatcagt tcacaccaga tgctattggc ttcattctgc tgtatctttt ggaacaattg
    20641 agtgataaag atagcttaga ggtacttgag attggaagtg gaacagggaa cctagcccaa
    20701 acccttctca acaacacgag caagagcctt gattatgtag ggattgaact tgatgatctc
    20761 ttgattgatc tgtcagccag tattgctgag ataatggatt cttcagctca ttttattcaa
    20821 gaagatgcgg taaggcctca attactaaaa gaaagtgaca ttgtcatcag tgacttacca
    20881 gttggttatt atcctaacga tgatattgcc aaacggtaca aggtggctag ttcagataag
    20941 catacctatg cccatcattt attaatggaa cagtctttaa aatacttgaa aaaagacggt
    21001 tttgcgattt ttctggcacc agtcaattta ttgacgagcc ctcagagcca gttattgaaa
    21061 cagtggttaa aagattatgc tcaggtggtg accttgatta cgctaccaga ttctattttt
    21121 ggtcatccct caaatgccaa gtccattatt gtcttacaaa aacaaacaga ccacccaatg
    21181 gaaacctttg tctatccaat tcgggatttg aagcttgcag agaatattca tgattttatg
    21241 gaaaatttca aaaaatggaa actgagtaat gtcaattaat tgattttttg ttataataaa
    21301 aaagcatcac agaaaacgat ttcaagagag gttaaaaatg tcaaagacaa ttgcaattaa
    21361 tgcaggtagt tctagtttaa aatggcaact ttatcagatg ccagaagaag aagtgttagc
    21421 acaaggaatt attgagcgta tcggtctcaa ggattctatt tcaacagtta agtacgatgg
    21481 caaaaaagaa gagcaaattc ttgatattca cgatcataca gaagctgtta aaatcttatt
    21541 gaacgatttg attcactttg gaatcattgc agcttatgat gaaattacag gagttggaca
    21601 ccgtgttgtt gctggtggtg aacttttcaa agaatccgtt gtggttaatg acaaagttct
    21661 tgaacagatt gaagaattat ccgttttagc tccacttcac aatcctggag cggcggcagg
    21721 gattcgtgcc ttccgtgata ttttgccaga tattaccagt gtttgtgtgt ttgacacgtc
    21781 atttcacact agcatggcaa agcatactta cctttaccca attcctcaaa aatattatac
    21841 ggactacaag gttcgtaaat atggggcgca tgggacaagt cataaatatg tagcgcaaga
    21901 agctgctaaa atgcttggtc gtccattaga agaattaaaa ctaattactg ctcatattgg
    21961 aaatggtgtc tcaattacag ctaattacca cggtaaatca gttgatacct cgatgggctt
    22021 tacaccactt gctggtccta tgatgggaac acgttctggt gacattgacc cagctatcat
    22081 tccttattta attgagcaag acccagaatt aaaagacgct gctgacgttg tgaatatgtt
    22141 aaacaaaaaa tcaggtctta gtggggtgtc aggcatttct agtgatatgc gtgatatcga
    22201 ggctggttta caagaagata atccagatgc tgttttagct tacaatatct ttattgatcg
    22261 cattaaaaaa tgcattggtc aatatttcgc tgttttgaat ggagcagatg ctttggtctt
    22321 tacagctggt atgggtgaaa atgcaccatt gatgcgtcaa gatgttattg gtggcttaac
    22381 atggttcgga atggacattg atcctgagaa aaatgttttt ggataccgtg gtgaaatttc
    22441 aactcctgag tcaaaagtga aggttcttgt tatctcaaca gacgaggaat tgtgcatcgc
    22501 gcgtgacgtt gagcgtttga aaaatacaaa ataaaagaga ctaagttttc atagattaac
    22561 caagtaaggc aacactttac ttggtttttt atgaggttta tctgttagtt atggtgccta
    22621 ggtattagtt ctgatcagtc tccttgcttt ttgtaatctt gtgctaggcg atttggatgt
    22681 cagccttatt tagaagtttt taattgcttc aacgcttttc ttgtatgtgt ttatagagcc
    22741 caatgatcta aaagactatt tgataacggt ttatgatgag cgtaatcgtg cgattcgaaa
    22801 acagacacaa gtttactgct tttacctgat gattgttcta tttgttgcct tattcttttt
    22861 cttcccttgc ttaaccattc gagaccttgt ttcttataag ctagctttga tttttgctta
    22921 cctttacttg attatcagaa gtctggtggc taagaaaatg gaggcactag ggaaaataac
    22981 aactaatagt caaaaacctc cgtttgatac gagtcaggtt ctttgttaga tgacttgtct
    23041 aacggttgag tttcctgtct ggtcaacgga ggtgtttgta ttgttgtctg atttgtattg
    23101 gggcacttat tttacaagct tttagcctta tcgatggttt tgtcaatggc agagctgaca
    23161 gtagttgtga ggccaaggcg ttctaactcc attagaccag caatagttgt gccaccgggg
    23221 ctacaaatag cgtcaatgaa atcgtgcgga ctttgagaac tggtcttgag attgctggcg
    23281 ctagccaaga ctgtttgagt aacaatctcc agcgcctttg ctttaggcat gccattcttg
    23341 acgccagcct tagccaaagc ctcaataaag agataaatat aggcaggact tgaccctgct
    23401 aaagcggtaa aggtgtcaaa atccttttca ctaatatcaa atgtgctacc aaagctatct
    23461 gttaagtttc gaacacgtgc ttgtaattcc tgggacacca aagcatttcc cgttaaagcg
    23521 gtactgcttt ggagaatttg tgcattcata tttggcatga tacgtagcag cggaaggtct
    23581 tgtcctacga atgttgctag tcgttgaagg gaaatgcctg ctgccataga tataataggc
    23641 tgtttgaagt gaagcggttt gagtaccgtt tcaaatagtt gaggcttgat gcctaaaata
    23701 acaagatcaa cctggtcaat aaggtcttgg tgggacatag cataaggcag tgttaactgc
    23761 tctgcaattt ccttggaccg ttctaaagat gatcctgaaa tgataagttc atggggtgtt
    23821 tgtttaaggc ctttgatgat agcgctagcc attttgccaa caccaataat gccaattttc
    23881 attctgttgc tccttgtggg atattacggt taatcaggct aacgctagtt tagtaacgtt
    23941 taatcaagtc aacggttgag cgatccagtt ttttgataat ggcttgtaag aaggcttggg
    24001 cttctacgaa atcatccata gcgtagaggg tttgatgaga gtgaatgtag cgtgcacaga
    24061 ctccgatggt tgttgatggg acaccaccat tttgaaggtg tgcagcacct gcatctgtgc
    24121 ctcccttgcc acaatagtat tggaaattga caccagcgtc ctcagcagta gtcagtaaga
    24181 agtcgcgcat atctttgagc atgacatggc ctgggtcgta aaagcgcaat aaggtaccat
    24241 ctccgattgt tccaggattg ccataaatat caccagcagg cgaacaatct actgcgaaaa
    24301 agagttcagg gtcgaacttg gtggttgaga cgtgggctcc gcgcagacca acttcttctt
    24361 gaacgttagc acctgcaatt agggtgttgt taaggtcttg tccttttaac gcttcaagca
    24421 tttctgttat catgagaacg ccatagcgat tgtcccaagc ttttgaaata atattttttt
    24481 gattggctgt taggatcgtt tcagattgag ggacaataat atcacccggt gtaatgccaa
    24541 agctttcagc ttctgcctta tccgtaaagc caccatcaaa cacaatattt tcgatatttg
    24601 gtagactagc agagccattt gccccacgta aaaaatgggg aggtaccgat cctgaaataa
    24661 ggggaataac ttggccagtg cgtgtgtaaa gggtaaaccg ttgtgaactg acaacaagtg
    24721 ggttccaacc accgatacca accacgcgta gcgttccgtc aactttgata tcactgacca
    24781 taaaaccgac ttcgtccatg tgcgcagcta ctaaaatacg gggggctttt tcagctttac
    24841 tatctctgat accaaaaatg ccaccaagcc cgtctgtttc aacacggtca accagcgggg
    24901 ttattttggt gcgtaggtag tcacgaacgc tatgttcata gcccgcaatg ccatccagtt
    24961 cggtaacttc tttgattttt gagaataagt ctgtcatttt tcatctccat ttaaaaggtt
    25021 tcagaccagt tcaacgcggt catctcctta tattctacca ctttttaagt aagaatgggt
    25081 ttcacagaaa agaaaaatcc cagagactag agatctctgg aagaagtgtt tgtcttttgg
    25141 cgataacctg tttttttagg cagcaaggaa atcattaaaa tgataaagca caagctggtt
    25201 aataagaata gtagggcgct taggatgcga aaagactctt cactagcgaa aagtaaggat
    25261 aatggagtca taaggagaac cttccatttt aggttaatca gtatttttta tagcgtggtt
    25321 aaattaataa taaaccacac ctaatcaatg tacttttagt ttagttgttt ttactatttt
    25381 tgtcaataca taacagtaga aatctttagt tttcttattt cagatcgtcc aatttgatct
    25441 ttttatgata aaatgaggga tatgagtaaa aagaaaatag gtatgatttc aggtatcttt
    25501 ggatttagtt tagctattgg cctgggaata gttatcaaag attattgcca agacaggcag
    25561 cgccgacaaa tgacaaggga tttacgcacc tttttttcac ctttaggaca aatcgaagtt
    25621 ttatatatca atccttgtca ggtcaaacaa gattatattt caggtggggt tgttatgtca
    25681 aacggcaaac agtaccaatt cacctatcac agtcgacaaa ttagttttga ggaggacaag
    25741 ggatgatcag accgacttct tatgagtcat tagcggctct tattgaaaaa gaagataagc
    25801 tggtgttatt ttttacggca gactggtgtc cagactgtca atttatttat cctattatgc
    25861 cagaaattga agcagaactt acagatatga cttttatttg tgttaatcgt gatcagttta
    25921 tagaggtagc acaaaagtgg aatatttttg gtattcctag ttttgtcgtg attgaaaaag
    25981 gtcaggaagt agggcgtctg gttaacaaaa tgagaaagac aaaaactgag attatgcatt
    26041 ttttagctgc ttatcaataa gaaaagagga agaataaatg atttttgcat acaataaaga
    26101 acaagttggc gatgtcttga tggttatctt acaagacacc aaagatatca aacgtcaagt
    26161 agaacgaaaa ggcaaagtag cccgtgtttt tgcagaagaa agcggcaaaa cccttgcttg
    26221 gaatatcttt gaagtgtcaa gcttgattac tattgaaggc aatggacaga tttttttgac
    26281 agacgaggac cttgcaagat taaatgcaga gcttgccaag gaaggatttt cagaaaggct
    26341 tgaaccgact gtgggacctg tttttgtggt tggtcaaatt gttgagatgg tggctcatcc
    26401 agatagcgac catcttaata tttgtcaagt ggctattggt gaagatcaaa cggttcaaat
    26461 cgtagcgggc gcaccaaatg ctgcgcttgg tttaaaaacg attgttgcct taccaggtgc
    26521 tataatgcca aatggtagtc tgattttccc aggaaaattg cgtggtgaag aaagttatgg
    26581 tatgatgtgc tctcctcgtg agttggtctt gccaaatgca ccgcaaaaac gtggcattat
    26641 tgaacttgat gagtcagctg tggtaggaga agcttttgac ccagccaaac attggaaagg
    26701 ttagaaaaac cagctgaaat aagactgtat ttaactaaat gaccagaaaa aaaggattag
    26761 gagcccttaa tccttttttc ttatgttttc aaaaacgttt aaaatgaaac ttattttacc
    26821 acaattttta cgaataataa gataaggagg tcagtatgta taataaagtg atagcaatcg
    26881 gtcgtttggt agctaaacca gaattggtaa aaaccgctac ggataagcat gtagcacgtc
    26941 tctctttagc tgttaatcga agatttaaaa atgcttcggg agagcgagaa gcggatttta
    27001 tttcaattgt tgtttgggga aagttagcag aaactctggt ttcttatgct agcaaaggta
    27061 gtttgatgtc tattgatggc gaacttagga cccgcaagta tgataaagat gggcaagtgc
    27121 attatgtgac agaagttctc tgccaatcat ttcaactgct tgaaagtcgt gctcagcgcg
    27181 ctatgagaga aaataatgtt actaatgatc tagttgattt agtcttagaa gaagatactc
    27241 ttcccttttg atagaggcta atgcttctca tcagggttca gctgattact gaaatatatg
    27301 atgaggtgat atccgagcaa caaaagtgtg actagatact tctgataaaa ataaaaaaaa
    27361 caccttacga gtatgaacaa agtttcaagt gctagatgtt ggaactttgt tcatcatagg
    27421 aatagtaggg tgttttttag tgtattactt ctttttgttg ctagtgttaa ggtttacttg
    27481 cttacaagcg gttatcaggc cactagaact cttataataa atggagctct ttgagtttgg
    27541 tgtcaatcat ttttaagaca gtcgccaaat cttgtgggtt ttgaacgaaa tcaagggaat
    27601 tgccatcaat tttcattttt ggagagacct tgtagtcttc gtaccaggtt gggtattcac
    27661 cgtggacttg atggtaatat tgctcaagac taggattgcc gtcgacctgc tcaaaacttc
    27721 ggccacgttt ttcaatgcgc tcaagcattt tgtcaaaaga cacatcaatg tagatgagaa
    27781 gatcagggcg ttttttaggc atgccttcta gttcttctaa catgttggcg agaagttcct
    27841 gatagatgtc aagttctgtc ttagtgacat tgccattttt gtagttaagt tttaagaaga
    27901 gttcgtcttc aaaaatagag cggtcaagaa tgttgttatc ggcttgataa gcttctttga
    27961 tggacttaaa ccgtttattc aaaaaataaa tttgaagtaa aaatgcgtac tttttagggt
    28021 cttggtagta gaggtctagc acggggttat tatcaacagc ttcataaaaa acgtcagttc
    28081 ctaagtgctc tccaagtgca gcagcaagcg agctcttgcc ggccccaatt gttccagcta
    28141 gtacaatcaa catgcgtctc cttctaatat cattagcgta ctgttttgaa gatggcttca
    28201 acctcagcta gagtctcagc gcgtgaaaca gcaccacgaa ctttggcagc acctgctgtt
    28261 ccgcgaagat agtgtggcgc taagccccga aattcccgaa cagcaatggt ttcacctttg
    28321 aggttaatca ggcgtttgag gtggtctttg gcgatgtcta attttttggc aaagggaaga
    28381 tctggcaatt cttgaccagt ttcaaagaaa tgattgattt gggtaaaaag ataaggattg
    28441 ttcatggctg cgcggccaat catcacggca tctacaccga tttcttcaat cataaatttg
    28501 gcgtcttgca ccgaacgcac gtccccattt ccaataaagg gaatcttagt gatagcctta
    28561 gaaactcggg ctaatgtctc atggtcgcag gttcctgtat acatttgttc acgggtacga
    28621 ccgtgcatgg caagagcaga tacacctgcc gactcagcgg caagggcatt ctcaacggca
    28681 agtgaactat ccgcccaacc tgtacgcatt ttgacagtca gtggaatatc taaaacagac
    28741 gtgacttcct tgacaatgtg gtaaatctta tcagggtcac ggagccactt ggcaccagcc
    28801 tcgtttttga caactttatt aacagggcag cccatattaa tatcaacaat atcggccttg
    28861 gtattggttt gaatgaaatc agctgcacgt ttcaaccctt ctgcgtcacc accaaataat
    28921 tgaatggaca tagggtgctc attctcgtca atatggagca tgtgaagggt cttttcgttg
    28981 ttgtagagca gccctttttc ggaaatcatt tccataacca ccaaaccagc accaaattct
    29041 ttggcgatgg tacgaaaggc agaattggtc acgccagcca taggggcaag tacagtgcgg
    29101 tgggggattt ccacatcacc aatcctaaag gaagaattaa gctttgtcat tgatgagggc
    29161 ctccaaatca ttttcattaa attggtactt ggtaccacaa aattggcaaa caatctcagc
    29221 acccttatct tcgtcaatca tggcctgtaa atctgctttt ggcaaagtca ttagggctgc
    29281 ttcaaaacgt tctctagaac agtcacactg aaaactcaga ggttcttcag aaagacgctt
    29341 gtaaggttca tctccataga tggcctccaa aagggcatcc acatgatttt tggaagctag
    29401 taggtgagaa atggcaggca tttcttgcag gcgtttttca tagcgagcaa tctcttcttc
    29461 agaagctcct ggtagcacct gcaccataaa gccaccagca accttgacct tatcattttc
    29521 atctaaaagc acattaagtc cgattgcaga tggtgtttgc tctgattcgg ttaagtagta
    29581 ggcaaaatcc tcaccaattt ccccagtaat aagaggcgtt gttgaggtgt aagggttacc
    29641 tgtgccataa tcaataatcg tcacaaaatg accattcccc ataaaaggcc caacaaggac
    29701 ttcacctgtt gcggtctttt taatatcgac accagtattt tgaatgtaac ctttcacatg
    29761 tcctttggta tctgccacag agataatgtg tccaaaagaa gaatcaccaa tcactttgac
    29821 ggtgatttta ctatcgcctt tttgattagc cgccaaaatc tggttggcga tcaaggtacg
    29881 gccaagcgca acagtagacg atgacagcgt gttgtgtttt tcttgagcta atgcaaccgt
    29941 ctcagtgcta tcaaggacat aagccctaaa agcccctgat tgtgctattg atttaattat
    30001 tttatccata acttagatta tagcataaag tgtaaaactt gtattgacag gattgcttga
    30061 aaaatcaata cagatagaca gtaaaaaatc ccagagaatg actcgggatt aagttaaggt
    30121 gtggtctaaa gtcgcgcaat gtggtgacat ggtgcttatg ttaattgctt ggttaaatca
    30181 gcttggaatt tttcctcttt aacttgatac atcaattctt ggatagacag aatcgattca
    30241 tcaaaagata tttcagcaac agcaattcct tttgtaagtt catggtgaac aaaaggaatc
    30301 agttgactgt gagtgatgac caaatctggc tttaaatcag gaatttgata aacattatct
    30361 tgcaatagat aataggaatg aaaatcaatg cttttatccg agaaatacct tggaaaagaa
    30421 tccgttagga gatgagcatt gataaaatta ctggctacga aaacaacaac taaaggaggt
    30481 tggatatttc ttagactttg ctcgatatag tgacaaaaaa gatgaaaatg cttatggttc
    30541 aagtcacgct taccaggaag tttggccatc cactcttcga caattaactt taaggacgta
    30601 tagagttttt ggtttccttt atagtacgga gaaacgaata agttggtctc aggaataaaa
    30661 tgttgcagat taaacaagaa tgattttgaa aaaaacataa gagcttttac taaactagcc
    30721 ttttgctctt ttaggttagg taaaagagtg atgataggat ttaaaagcag gcgaaaagta
    30781 tcattttctt caaaaagttg acaacattgt ctgatatgct caggtgtcca ttgtaagctc
    30841 gcaaaagaat tattagcggt gatataaatt aaatagaggt agtccaaatc tcctgctgaa
    30901 aagtttagtt ggcagtaagt ttcgataata tcatggctac tttttttcaa agaatcatag
    30961 acaaaaagtt tttttaattg ttgaaaaatt ctggtttggg gaatagttac cgaaaattga
    31021 tgccgcttcc acgataaagc caataaaatg tcatagaaag aaaacgattc cgataaccaa
    31081 ggagaggttt taaggtgggt ggaactatgg gataaaaagc tatgaatagt gtttttgtct
    31141 tgctgcgtca agtcataaac tttaatgcca aacttactat atagcagagc gatgaggtaa
    31201 cggatgcgat attcctcacc gacaatcttg ttcttagaga gttttaattc aaagtttctt
    31261 aataaaggaa tcaatgcttc gcgcatccga taagctgagg agtttgataa aaaatgactt
    31321 cttgcaaaat ccgtaagggg acgagagtgg gaaccatttt ttattaaaaa cgctagtaat
    31381 tgtaagacat tagatgatgc atagagttgg taaagataag tttctttaaa aggatgtgta
    31441 aattggcaag atatcatcct tttttggatg gtcatagaca gactaccagg gaaaaaggca
    31501 ttcagttcct cacaataatg gtttagttgt aaaaaggtta agccagtttt ttctgctacc
    31561 tcagttattg gcaaataaga tgtcttaaaa aacaagataa ttaactgaca ttttgattcg
    31621 attgatgatt ccaagtattt ttctatcaag gaaagggacc tctcttccta aacttagaaa
    31681 gccaaataag tctttgataa gtgtattctc ctatttcaat tctatctaag ctaaaaatag
    31741 aaatcaaatc taatggtttt tggtgaaaag tcgaaacctt ttgaaatagc tttaaagcaa
    31801 tattttctca aaaaatcatt ttcaaaaaca ttaatttggt gaaaaatata atcaaaaaat
    31861 tgtctttctt gacaataacg tggtaagctc atatatgttt ttgagaggag agaaaatgaa
    31921 taacaaaata tttttgaata aagaagccgg ttttttggca cacacaaaaa gaaaaaggcg
    31981 atttgctgtc actttagtgg gagtcttttt tatgcttttg gcatgcgcgg gtgctatcgg
    32041 ttttggtcaa gtagcctatg ctgcggatga gaagactgtg ccgaatttta aaagcccaga
    32101 tccagattat ccctggtatg gttatgatgc ttatactgga gcgtttttaa gatatcatga
    32161 tcttagagtc aatttaaacg gaagtcggag ctaccaagtt tattgtttta atattaaaaa
    32221 aaactatcct cgacctttta cgagtagtga taaaaaatgg tataaaagat tagagggaac
    32281 tgctgaaact tttaaagttc atgctatggc tcccagagta gggggagagg aactaactaa
    32341 aaaattacgg agcgttatgt ataacggcta ccctaatgac ggcaataata ttatgaaagg
    32401 tttggagcct tcaaatgcta tagaagtaac acaatacgca gtttggtact attcagataa
    32461 gtcacagtat gaaattgaga cattatggaa gtctgaagcg aaagaaggta agattagtga
    32521 gtcacaagtt actttaatgc gtgaagccct gaggaaacta atttcctctg atgaagattt
    32581 ggttaaacaa gttccatcga actttaaact tagtactttt gagtcttctg ataagtccta
    32641 ccaaaatctt ttaagtgctg agtttgtacc tgatgatccc cctaaacctg gtgatacgtc
    32701 agaacataat cctaaaactc ccgagttgga tggtagtcct attcccgaag acccaaaaca
    32761 tccagatgat aatttagaac ctacattgcc gccagtgatg ctagatggtg aagaagttcc
    32821 agaagttcca agcgagagct tagaacctgc gcttccccca ttgatgccag agctagatgg
    32881 tgaagaagtc ccagaagttc caagcgagag cttagaacct gcgcttcccc cattgatgcc
    32941 agagttagat cgtcaagaag tccctgaaaa acctagtgtt gacttaccta ttgaagttcc
    33001 tcgttatgag tttaacaata aagaccagtc acctctagcg ggtgagtctg gtgagacgga
    33061 gtatattacc gaagtctatg gaaatcaaca gaaccctgtt gatattgata aaaaacttcc
    33121 gaatgaaaca ggtttttcag gaaatatggt tgagacagaa gatacgaaag agccagaagt
    33181 gttgatggga ggtcaaagtg agtctgttga atttactaaa gacactcaaa caggcatgag
    33241 tggtcaaaca actcctcagg ttgagacaga agacacgaaa gagccaggag tattgatggg
    33301 aggtcaaagt gagtctgttg aatttactaa agacactcaa acaggcatga gcggtttcag
    33361 tgaaacagtg accattgttg aagatacgcg tccgaagtta gtgttccatt ttgacaataa
    33421 tgagcccaaa gtggaagaga atcgggaaaa gcctacaaaa aatataacac ctatccttcc
    33481 tgcaacagga gatattggaa atgttttggc ctttcttgga atccttattt tgtcagtact
    33541 ttctattttt agccttttaa aaaacaaaca aaacaataaa gtctgacgta aaagtgttcc
    33601 ataagaaata atgcgcaaat atgctgaata gtggataact gctacagtca agacaagtgt
    33661 taattcaaaa ggtactactt atacatctga taataataag gtgacagata attcagagca
    33721 agtaggatgg ctaaaaaatg atgtattcta tgcataatag acttacaaga aatcaagtta
    33781 ttgttaaacg tcgtaaagct taacgctttc aaaagtcgaa atcaatcgag actgtcttta
    33841 ggcagtctcg atttttattg ataaggaact gcaatgacag aaagactaaa aaatctaggg
    33901 atactcttat tatttttatt gggaacagcc atttttcttt accctacgct aagtagtcag
    33961 tggaatgcct accgtgatcg tcaattgtta tcaacttatc acaaacaagt gactcaaaag
    34021 aagccctcag aaatggagga ggtgtggcaa aaggctaagg cgtataacgc taggcttggg
    34081 attcagcccg ttccagatgc tttttcattt agggatggca ttcatgataa aaactacgaa
    34141 agcctacttc aaattgaaaa caatgatatt atgggatatg ttgaagtgcc ctctattaaa
    34201 gtgacgttac ccatatacca ctatacgaca gacgaggtgc taactaaggg agcaggtcac
    34261 ttagttggaa gtgccttacc agttggtggc gacgggactc acacggttat ttctgctcat
    34321 agaggccttc catcggcaga aatgtttact aatttgaatt tagtgaaaag gggagatact
    34381 ttttactttc gtgtgttaaa taaagtattg gcttataaag ttgatcagat actaactgtt
    34441 gaaccagatc aagtcacatc tctttctggg gtaatgggaa aagattatgc tactctggtt
    34501 acctgcacac catatggtgt taataccaag cgtctattgg tcagaggtca tagaattgca
    34561 tatcattaca aaaagtatca acaggctaaa aaagcaatga aactcgtgga taaatcaaga
    34621 atgtggacag aagtagtatg tgctgctttc ggggtggtta tagctattat tttagtattt
    34681 atgtattcaa agatgactga taaaaaagtg attaaaaact aacttgccct gaaccttctt
    34741 tatcgtattg caactgtcat aaaagtaaaa taatctggta gttactatcc cctttgatga
    34801 agtttaatta aatctcatca agagaaaaat attaggcgat tacaaatgat tttcccaaaa
    34861 attatttttt ggaacgcata atatggaaaa aaagatgata agaacaactg ttttaaacat
    34921 caaataaatg atatagtagc tatgaaatag cattgtgact gtccggtaaa gggatgggac
    34981 tgaagatttt attcagaagg agagggaatg aataacaaaa aattgcaaaa gaagcaagat
    35041 gctcctcggg tatcaaacag aaagccaaaa caattaactg tcactttagt gggagtattt
    35101 ttaatgctct tagtattgat aggatttgaa ggaaaagttc gagcagctca cgaattggtt
    35161 gaggtaccag tacctatttt tcacaatcca gatccacagt cagactatca gtggtatggc
    35221 tatgaagcct atacaggggg gtatccgaag tatgatttat tcaagacata ctatcatgat
    35281 ctgagagtga atttgcatgg gagtaaaagt tatcaagtat attgctttaa cgttcacaaa
    35341 cactatcccc gttcctccca gagttttgat cggaagtggt ataaaaaatt agatggaact
    35401 gctgagaatt ttgattctct tgctatggaa ccaagagttc gtaaagagga gttaaccaaa
    35461 aaactccgag ctgttatgta taatgcctat cctaatgatg ctaatggcat tatgaaagat
    35521 ttagagcctt taaatgctat taaagtcact caagaggctg tatggtacta ttctgattca
    35581 gctcaaatta atccggatga aagttttaaa acagaagctc aaagtaatgg tattaatgac
    35641 cagcagttag gcttaatgcg aaaagcttta aaagaactaa ttgatccaaa cttagggtca
    35701 aaatattcga ataaaactcc atcaggttat cggttaaatg tatttgaatc tcatgataag
    35761 actttccaaa atcttttgag tgctgagtat gttccggata ctcccccaaa accaggagaa
    35821 gagcctccgg ctaaaactga aaaaacatca gtcattatca gaaaatatgc ggaaggtgac
    35881 tactctaaac ttctagaggg agcaactttg cgtttaacag gggaagatat cccagatttt
    35941 caagaaaaag tcttccaaag taatggaaca ggagaaaaga ttgaattatc aaatgggact
    36001 tataccttaa cagaaacatc atctccagat ggatataaaa ttgcggagcc gattaagttt
    36061 agagtagtga ataaaaaagt atttatcgtc caaaaagatg gttctcaagt ggaaaatcca
    36121 aacaaagaag taggttctcc atatactata gaggcataca atgattttga tgaatttggt
    36181 ttactgtcaa cacaaaatta tgcgaaattt tattatggaa aaaactatga tggcagttca
    36241 caaattgttt attgcttcaa tgccaacttg aaatccccac ctgactcgga agatcatggt
    36301 gctacaataa atcctgactt tacgactggt gatattaggt atagtcatat tgctggttca
    36361 gatttgataa aatacgctaa tacagctagg gatgaagatc ctcaattatt tttaaaacac
    36421 gtaaaaaaag taattgaaaa tgggtatcat aaaaaaggtc aagctattcc atataacggt
    36481 ctgactgagg cacagtttcg cgcggctact caactggcaa tttattattt tacagatagt
    36541 gttgacttaa ctaaggatag attgaaagac ttccatggat ttggagatat gaatgatcaa
    36601 actttgggtg tagctaaaaa aattgtagaa tacgctttga gtgatgaaga ttcaaaacta
    36661 acaaatcttg atttcttcgt acctaataat agcaaatacc aatctcttat tgggacagaa
    36721 taccatccag atgatttggt tgacgtgatt cgtatggaag ataaaaagca agaagttatt
    36781 ccagtaattc atagtttgac ggtgaaaaaa acagtagtcg gtgagttggg agataagact
    36841 aaaggcttcc aatttgaact tgagttgaaa gataaaactg gacagcctat tgtcgacgct
    36901 ctaaaaacta ataatcaaga tttagtagct aaagatggga aatattcatt taatctaaag
    36961 catggtgaca ccataagaat agaaggatta ccgacgggat attcttatac tctgaaagag
    37021 actgaagcta aggattatat agtaaccgtt gataacaaag ttagtcaaga agctcaatca
    37081 gcaagtgaga atgtcacagc agacaaagaa gtcacttttg aaaaccgtaa agatcttgtc
    37141 ccaccaactg gttttactac tgatggtgga acctatctgt ggttattatt gcttgtccca
    37201 tttggtttgt tagtgtggtt ctttggtcgt aaaggactaa aaaatgacta attacctaaa
    37261 tcgcttaaat gagaatccac tatttaaagc tttcatacgg ttagtactta agatttctat
    37321 tattggattt ctaggttaca ttctatttca gtatgttttt ggcgtcatga ttgttaacac
    37381 aaatcagatg agtcctgctg taagtgctgg tgatggagtc ttatattatc gtttgactga
    37441 tcgctatcat attaatgatg tggtggtcta tgaggttgat aacactttga aagttggtcg
    37501 aattgccgct caagctggcg atgaggttaa ttttacgcaa gaaggaggac tgttgattaa
    37561 tgggcatcca ccagaaaaag aggtccctta cctgacgtat cctcactcaa gtggcccaaa
    37621 ttttccctat aaagttccta cgggtacgta tttcatattg aatgattatc gtgaagaacg
    37681 tttggacagt cgttattatg gggcgttacc catcaatcaa atcaaaggga aaatctcaac
    37741 tctattaaga gtgagaggaa tttaaaaaaa tttggagaga gagaaaatga aaaaaaataa
    37801 attattactt gctactgcaa tcttagcaac tgctttagga acagcttctt taaatcaaaa
    37861 cgtaaaatct gagacggcag gagtgtccga aaatgcaaaa ttaatagtaa aaaagacatt
    37921 tgactcttat acagacaatg aagttttaat gccaaaagct gattatactt ttaaagtaga
    37981 ggcagatagt tctgcaactg ataaaacgaa agatggttta gagattaagc caggagtaac
    38041 tgaaggttta actaccgagc agacaattgc ttatgataac tcagttaaac catcggataa
    38101 atctaagact gcaacttttg atttttcaac cgtaaaattt ccagaagttg gtgtttatcg
    38161 ttatacggtt tcagaaatag atagcaaagt tagtgggatt aaatacgata ccaaaacatg
    38221 gatagtggat gtttatgttg ttaatgatgg taatggagga tttaaggcac ggtatattgt
    38281 atctaaagag aagggacaaa atgataaaaa gcctgttgtt tttgaaaact cctttaagac
    38341 aacttcttta aaggtagaaa aacaggtgac tggtaataca ggagaactaa agaaagactt
    38401 caatttcact ttgacaatta atcctaatga taatttcgta gctggacaag ttattaaatt
    38461 agagaaaggt ggaattaaag ctgatgttaa gattggtgag ccgtataaat ttgctctaaa
    38521 gaatggtgag aaagtaactt tatcaaaatt accagtaggt gttacttata gcatcattga
    38581 agatgaggca gataaagacg gatacacaac taatgccaaa attacagatg gtacggcagc
    38641 gcctgtggaa tataagttag gaaatcaaca attggctgac gaatctgctg acgaaatcgt
    38701 tgtcacaaat aaccgtgaca ctcaagttcc aactggtgtt gtaggcaccc ttgctccatt
    38761 tgcagttctt agcattgtgg ctattggtgg agttatctat attacaaaac gtaaaaaagc
    38821 ttaagtgatt agtcaaagaa tgatgatgac aattgtacag gttatcaata aagccattga
    38881 tactctcatt cttatctttt gtttagtcgt actattttta gctggttttg gtttgtggga
    38941 ttcttatcat ctctatcaac aagcagacgc ttctaatttc aaaaaattta aaacagctca
    39001 acaacagcct aaatttgaag acttgttagc tttgaatgag gatgtcattg gttggttaaa
    39061 tatcccaggg actcatattg attatcctct agttcaggga aaaacgaatt tagagtatat
    39121 taataaagca gttgatggca gtgttgccat gtctggtagt ttatttttag atacacggaa
    39181 tcataataat tttacggacg attactctct gatttatggc catcatatgg caggtaatgc
    39241 catgtttggc gaaattccaa aatttttaaa aaaggatttt ttcaacaaac ataataaagc
    39301 tatcattgaa acaaaagaga gaaaaaaact aaccgtcact atttttgctt gtctcaagac
    39361 agatgccttt gaccagttag tttttaatcc taatgctatt accaatcaag accaacaaag
    39421 gcagcttgtt gattatatca gtaaaagatc aaaacaattt aaacctgtta aattgaagca
    39481 tcatacaaag ttcgttgctt tttcaacgtg tgaaaatttt tctactgaca atcgtgttat
    39541 cgttgtcggt actattcaag aataacgaaa ggaggagact tttgagaaaa tattggaaaa
    39601 tgttattttc tgtcgtaatg atattaacca tgctggcctt taatcagact gttttagcaa
    39661 aagacagcac tgttcaaact agcattagtg tcgaaaatgt cttagagaga gcaggcgata
    39721 gtaccccatt ttcgattgca ttagaatcaa ttgatgcgat gaaaacaata gaagaaataa
    39781 caattgctgg ttctggaaaa gcaagctttt cccctctgac cttcacaaca gttgggcaat
    39841 atacttatcg tgtttatcag aagccttcac aaaataaaga ttatcaagca gatactactg
    39901 tatttgacgt tcttgtctat gtgacctatg atgaagatga gactctagcc gcaaaagtta
    39961 tttctcgaag ggctggagac gaagaaaaat cagcgattac ttttaagccc aaacggttag
    40021 taaaaccaat accgcctaga caacctgaca tccctaaaac cccattacca ttagctggtg
    40081 aagtaaaaag tttattgggt atcttaagta tcgtattact gggattacta gttcttcttt
    40141 atgttaaaaa actgaagagt aggctataaa acccattttt aagagtcaca catttttagt
    40201 tgctctattt ttataatgaa acttattgac atctccatag tcttgcttga ctttggagat
    40261 gttttctatg ctgataggtt tttgtaccat ataagataat gattgatgaa aagagtgtct
    40321 tgaattctta aatattatct cggtatttag caagatagtc ttttgaagaa atacccgtgt
    40381 attttttaaa gattttatga aaatgggtag tgtcataaaa atataagctt ttagccactt
    40441 ctccaacagg aattcctcgt tttaagagta gttgagcttc tttgattttt gtacttagaa
    40501 tataatgttg taaggaaaca tttgagtatt ttttaaagac tgaacgtaag tgagattcgg
    40561 aaacatatag gcgcttagcg atatcagata cctttaattt ggaatacagg tgactatcga
    40621 cataatgaag cacactattg tacatttgac gtttatcaga aatactttta ttggttaatg
    40681 attcggaaaa atggatgata gctgcacagc gaattctcaa aactttgagc aagtcactcg
    40741 cttcttcatt taaggccatg tagtatttga caaggctata atgctttgct acgtcagtat
    40801 tttctacaaa actgaattcc agtaacctag ttaaatagat aacagtgagg ttcttttccg
    40861 cccttagagc agaaatagaa gatgaggtga tacttgataa ggggatgcga ttgatttctt
    40921 gcttgagcaa ttgtggattg cctagcttta ccagatccat aatatgattt aggtgctcta
    40981 aaacaggcgg taattggcta gatgctttgc taatatgctt aaggcgatga atgatatcag
    41041 gatcagcagt catctgtttt gaatgctgca acaattgatg aattgtttgt tggcaagtcg
    41101 tctcaaattg tgtgtcaaaa aaagcgtcaa tagctatcaa aaggttccgc acattattaa
    41161 ttggaaaatg tgggacaagt gccatatagg acaagagctc ttctttgcta cgatgagaag
    41221 aatgttttaa aaaattatta gctaattgtt cctgatagtc tttattaagt gaataagggt
    41281 agaaaggtcc aatggcaata atgtagtgac aaagaggaaa aatcataaag gactcttcaa
    41341 ataaaccctc ataaaaaata acatcttgtg cggcttgagg aatagctaga tgctttaaaa
    41401 attgatagta acataataaa tagtcgtcat taccatatac ttgaataagt gccttatctt
    41461 gtgacatcac tgaaataggt aattgagaca agctgtgtaa tgtttgcaca tattttaaat
    41521 cgaatataac catttgatag attgcccccc atccttagtt gtatcatttt ccttagactt
    41581 gtaaaagctt tagtaaatgt taatgatgaa aattcctatt tggtaggcac actaaaaggt
    41641 tttcctaata gtaaactctc tctccatcat ctcctacctt aattatacta taaatagcta
    41701 agcagataaa gataaaggat ttgtacgtta agcgcttgaa aaagaaagag ttacagataa
    41761 tgacataaaa aacgctaaaa aaccatcaaa ataaatactc tgaccataag atgtagactt
    41821 gacaactgaa aatggtaaaa taactatttg acagttgtcc tgtagtcttt agttttggac
    41881 ataggctgtc gcttatgaat gtggagagag aaaataaatg acacaaaaaa atagctataa
    41941 gttaagcttc ctgttatcct taacaggatt tattttaggt ttattattgg tttttatagg
    42001 attgtccgga gtatcagtag gacatgcgga aacaagaaat ggagcaaaca aacaaggatc
    42061 ttttgaaatc aagaaagtcg accaaaacaa taagccctta tcgggagcaa ctttttcact
    42121 gacaccaaag gatgggaagg gaaaacctgt tcagaccttt acctcaagcg aagagggtat
    42181 tatagatgct caaaatctcc aaccagggac ttatacctta aaagaagata aagcaccaga
    42241 tggttatgat aaaaccagtc ggacttggac agtgactgtt tatgagaacg gctataccaa
    42301 gttggttgaa aatccctata atggggaaat catcagtaaa gcagggtcaa aagatgttag
    42361 tagttcttta cagttggaaa aatcaacaat gtctgtattc tctaaatacg gagggacaga
    42421 agtaactagt ggcgcagcgg atttctaccg caatcatgcc gcctatttta aaatgtcttt
    42481 tgagttgaaa caaaaggata aatctgaaac aatcaaccca ggcgatacct ttgtgttaca
    42541 gctggacaaa cgtctcaatc ctaaaggtat cagtcaagat atccctaaaa tcatttatga
    42601 cagtgaaaat agtccgcttg cgattggaaa atacgatgcc aagactcatc agctcaccta
    42661 tacttttaca aattatatcg ctggtttaga taaagtccag ttgtctgcag aattgagctt
    42721 attcctagag aataaggttg ttttagaaaa tcaaacgata tcagatttta agagtaccat
    42781 aggtgatcag gaaatcaaat atggtgggac agttaatgtc ctttatggaa atgcatctgg
    42841 gcaagacaac aattatgtta ctaacggatt gagcaatgtg ggtggaagta ttgaaagcta
    42901 caatacacaa acaggcgact ttacttggta tgtttatgtc aacccaaatc gtaccaatat
    42961 tccttatgcg accatgaatt tatggggatt tggaaggttg gttaaatcta ataactcaac
    43021 aagtaagtca gaggattccg ctacaagtag tgcccaattg gaaaaagtag aaatttatga
    43081 agttaaagag gactatacgc tacccactag ttatggggtt gatctaacaa aattgaattt
    43141 aagaaaagac cttgaggcaa agttaccaca agatagtact caaaattcta ataagcgctt
    43201 gcgaattgac tttggagata aacttcaaaa taaagccttt gttgttaaaa taacagggaa
    43261 aacggatcaa tctggtaagc cactagtcgt tcaatccaat ttggccagtt ttaataatga
    43321 aactgctgta acttatggta atcctgttaa taacgtctac ttccaaaacg aaattgcctt
    43381 gtctccttct aagggtagtg gttctgggac aagtgaattt actaagccct ctattacagt
    43441 agcaaatcta aaacgagtgg ctcagcttcg ctttaagaaa atgtcaactg acaatgtgcc
    43501 attgccagaa gcggcctttg agctgcgttc atcaaatggt aatagtcaaa aagtagaagc
    43561 aaaatcaaac acacaaggag aggttcattt taaggacctg acctcgggca cctacgactt
    43621 gtatgaaaaa caagcaccta aaggctatca gcaggtgaca gagaaattgg cgaccgttac
    43681 tgttgatact accaaacctg ctgaggaaat ggtcacttgg ggaagcccac attcgtctgt
    43741 aaaagtagaa gatagcaaag aagtcacgat tgtcaaccat aaagaaaccc ttacgttttc
    43801 agggaagaaa atttgggaga atgacagacc agatcaacgt cctgcaaaga ttcaagtgca
    43861 actgttgcaa aatggtcaaa aaatgtccga taaagttcaa gaagtaacga aggataacga
    43921 ttggtcttat cacttcaaag acttgcctaa gtacgatgac aggaatcagg agtataagta
    43981 ctcagttgaa gaagtgaaag ttccagacgg ctacaaggtg tcgtatttag gaaatgatat
    44041 atttaacacc agagaaacag aatttgtgtt tgaacagaat aactttaacc ttgaatttgg
    44101 aaatgctgaa ataaaaggtc aatctgggtc aaaaatcatt gatgaagaca cgctaacgtc
    44161 tttcaaaggt aagaaaattt ggaaaaatga tacggcagaa aatcgtcccc aagccattca
    44221 agtgcagctt tatgctgatg gagtggctgt ggaaggtcaa accaaattta tttctggctc
    44281 aggtaatgag tggtcatttg agtttaaaaa cttgaagaag tataatggaa caggtaatga
    44341 catcatttac tctgttagag aagtaactgt tccaacaggt tatgatgtga cttactcagc
    44401 taatgatatt attaatacca aacgtgaggt tattacacaa caaggaccga aactagagat
    44461 tgaagaaacg cttccgctag aatcaggtgc ttcaggcggt accactactg tcgaagactc
    44521 acgcccagtt gataccttgt caggtttatc aagtgagcaa ggtcagtccg gtgatatgac
    44581 aattgaagaa gatagtgcta cccatattaa attctcaaaa cgtgatattg acggcaaaga
    44641 gttagctggt gcaactatgg agttgcgtga ttcatctggt aaaactatta gtacatggat
    44701 ttcagatgga caagtaaaag atttctacct gatgccagga aaatatacat ttgtcgaaac
    44761 cgcagcacca gacggttatg agatagcaac tgctattacc tttacagtta atgagcaagg
    44821 tcaggttact gtaaatggca aagcaactaa aggtgacgct catattgtca tggttgatgc
    44881 ttacaagcca actaagggtt caggtcaggt tattgatatt gaagaaaagc ttccagacga
    44941 gcaaggccat tctggctcaa ctactgaaat agaagacagt aaatcttcag accttatcat
    45001 tggcggtcaa ggtgaagttg ttgacacaac agaagacaca caaagtggta tgacaggcca
    45061 ttctggctca actactgaaa tagaagacag caagtcttca gacgttatca ttggcggtca
    45121 ggggcaggtt gtcgagacaa cagaggatac ccaaactggc atgcacgggg attctggttg
    45181 taaaacggaa gtcgaagata ctaaactagt acaatccttc cactttgata acaaggaatc
    45241 agaaagtaac tctgagattc ctaaaaaaga taagccaaag agtaatacta gtttaccagc
    45301 aactggtgag aagcaacata atatgttctt ttggatggtt acttcttgct cacttattag
    45361 tagtgttttt gtaatatcac taaaaaccaa aaaacgccta tcatcatgtt aagataagct
    45421 gttggtgaca atagcgaaaa agggtccact tgttagtgga cctctttttc tggaatcgat
    45481 cgtctcggtt cactagataa gtcattttaa gtagttcaaa aaggcagagc cttaaaaagt
    45541 tttctgcctt tttaatcttg tggggcactt acgaaagtat ttcggccact tcaagcataa
    45601 taaaaagagt ctgagcaatc atgttgtctc agactctgac tgtaattatg caaaagatga
    45661 tggactggca gcttcattta ctcaccgaac tcccctttat aacttgggaa tgatgctgct
    45721 aatgggttgt tttgtttaat acctgctata aacggtaatt tatcccattt atctttgtaa
    45781 ttatcttcaa gctcagtttt aaacttccaa accattttat gagaaaaata agtgtgcgat
    45841 tgacggccgt aggtgtctac taaaacgtga gaaaattttc cgtcgttttt tagtttgtaa
    45901 aatttatcta gagaaggact ccagccagta taaccaggga atttatcaaa ttcttgatta
    45961 aaatttgttt gttggcttcc ccaatcataa tgccattgtg tattattgtg ttttttagaa
    46021 tcaactgtac cgttcccata atgatgctca taagttatct ccgtaggagc ttttacaccc
    46081 ttaggaagaa acactgagaa atcagggtaa acccattgtt ctttagctga attaaaggtt
    46141 acagtccatt cgatactgtt attgttatcg accaatgttt tagtatagtc gacatagtta
    46201 tcaacctagt aatttccctg ccagtaaagg tgcactttac ctttttcatc aatgttatca
    46261 tgggcatgta ctgatttata cgaactaaac agcatcgaag ctgctagtag tgaagtgaag
    46321 agatatttat tacatcgttt catatcatg
//
LOCUS       DATXIG010000010        40968 bp    DNA     linear   BCT 28-DEC-2023
DEFINITION  TPA_asm: Streptococcus pyogenes strain MGAS11113
            SAMN08555799-rid19682583.denovo.010, whole genome shotgun sequence.
ACCESSION   DATXIG010000010 DATXIG010000000
VERSION     DATXIG010000010.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN08555799
            Sequence Read Archive: SRR6740495
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pyogenes
  ORGANISM  Streptococcus pyogenes
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 40968)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 40968)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-OCT-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-OCT-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001918058.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001918058.2
            Genome Coverage       :: 368x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/12/2023 11:23:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 1,806
            CDSs (total)                      :: 1,769
            Genes (coding)                    :: 1,722
            CDSs (with protein)               :: 1,722
            Genes (RNA)                       :: 37
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1, 1 (5S, 23S)
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 47
            CDSs (without protein)            :: 47
            Pseudo Genes (ambiguous residues) :: 0 of 47
            Pseudo Genes (frameshifted)       :: 30 of 47
            Pseudo Genes (incomplete)         :: 20 of 47
            Pseudo Genes (internal stop)      :: 9 of 47
            Pseudo Genes (multiple problems)  :: 11 of 47
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..40968
                     /organism="Streptococcus pyogenes"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN08555799-rid19682583.denovo.010"
                     /strain="MGAS11113"
                     /serotype="Emm28"
                     /isolation_source="normally sterile site"
                     /host="Homo sapiens"
                     /db_xref="taxon:1314"
                     /country="Finland"
                     /collection_date="2002"
                     /collected_by="National Public Health Laboratory"
     gene            complement(<1..221)
                     /locus_tag="VC122_000549"
     CDS             complement(<1..221)
                     /locus_tag="VC122_000549"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011106857.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="shikimate dehydrogenase"
                     /protein_id="HEP5640494.1"
                     /translation="MLVLYVSSKLIKRRRMKFMYSALLGNPVEHSISDELFEYLNHNI
                     GVLDEYKHKKILVDSVCLEDKIKHLINDP"
     gene            complement(290..361)
                     /gene="shp3"
                     /locus_tag="VC122_000550"
     CDS             complement(290..361)
                     /gene="shp3"
                     /locus_tag="VC122_000550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_128650800.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide pheromone SHP3"
                     /protein_id="HEP5640495.1"
                     /translation="MKKISKFLPILILAMDIIIIVGG"
     gene            441..1292
                     /gene="rgg3"
                     /locus_tag="VC122_000551"
     CDS             441..1292
                     /gene="rgg3"
                     /locus_tag="VC122_000551"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002985634.1"
                     /note="Regulator gene of glucosyltransferase 3; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="quorum-sensing system transcriptional regulator
                     Rgg3"
                     /protein_id="HEP5640496.1"
                     /translation="MKSKLGATLRKVRKGKQISLCFVADEHLSKSQISRFERGESEIS
                     CIRLINILDKLHITLDEFLILHDEDYTNSETFANLVQYIRKQYSSQNINNIACLLSDT
                     SDYTLNSFEKTMVKSILHTMDSSIIPSDDELLQLTDYLFKVEKWGYYEILLLGNCVRT
                     INYNSYFLLTKEMLNNYIYSSRNKTNKRIVTQLAINCFILSINKEEFSNCSYLIDEIK
                     SLLDNELNFYEQTVFLYATGYFEFKRQLETGVEKMKQAIQVLDILNEDKLKLHYADHF
                     DKLVNKD"
     gene            complement(1545..5084)
                     /gene="smc"
                     /locus_tag="VC122_000552"
     CDS             complement(1545..5084)
                     /gene="smc"
                     /locus_tag="VC122_000552"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010921991.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chromosome segregation protein SMC"
                     /protein_id="HEP5640497.1"
                     /translation="MFLKEIELEGFKSFADKTKIEFYKGVTAVVGPNGSGKSNITESL
                     RWALGESSAKNLRGGKMPDVIFAGTQNRNPLNYAKVAVVLDNSDHFIKTAKKEIRVER
                     HIYRNGDSDYLIDGRKVRLRDIHDLFMDTGLGRDSFSIISQGRVEEIFNSKPEERRAI
                     FEEAAGVLKYKTRKKETQIKLNQTQDNLDRLEDIIYELDTQLAPLEKQAKVAKQFLEL
                     DANRKQLQLDILVKDIDIAQERQTKDTEALAALQQDLASYYAKRQSMEEDYQKFKQKK
                     QVLSQESDQTQTTLLELTKLIADLEKQIELVKLESGQEAEKKAEAKKHLEQLQEQLDG
                     FQAEEKQRTEQLLHIDQQLCDVKQQLNELSNALERFSSDPDQLMETLREEFVLLMQKE
                     AALSNQLTALKAHLDKEKQARQHKAQEYQLLVTKLDQLNDESQKAQAHYKAQKEQVEM
                     LLQNYQEGDKRVQELERDYQLKQERLFDLLDQKKGKEARKASLESIQKSHSQFYAGVR
                     AVLQSQKKLGGIIGAVSEHLSFDSDYQTALEIALGANSQHIIVTDEAAAKRAIAYLKK
                     NRQGRATFLPLTTIKARSLSEHYHRQLATCEGYLGTAESLVRYDDSLSAIIQNLLSST
                     AIFETIDQANIAARLLGYKVRIVTLDGTELRPGGSFSGGTNRQSNTTFIKPELEQISE
                     ELTRLVEQLKITEKEVAALQSDLIAKKEELTQLKLAGDQARLAEQRAQMAYQQLQEKQ
                     EDSKALLAALDQSQTTHSDESLLAEQARIEEALTAIAKKKSALTCDIDDIKENKDLVR
                     QKTQNIHQALSQARLQERDLLNEKKFEQANQSRLRTQLKQCQQNILKLESILNNNVSQ
                     DSIQRLPQWQKQLQDATEHKSGAQKRLVQLRFEIEDYEARLEETAEKITKESEKNDTF
                     IRRQTKLETHLEQVANRLRAYAKSLSEDFQMTLADAKEVTNSIDHLESAKEKLHHLQK
                     TIRALGPINSDAINQYEEVHERLTFLTSQKTDLTKAKNLLLETINSMDSEVKARFKVT
                     FEAIRKSFKETFTQMFGGGSADLVLTETDLLSAGIEISVQPPGKKIQSLNLMSGGEKA
                     LSALALLFAIIRVKTIPFVILDEVEAALDEANVKRFGDFLNRFDKDSQFIVVTHRKGT
                     MAAADSIYGITMQESGVSKIVSVKLKEAQEMTN"
     gene            complement(5085..5777)
                     /locus_tag="VC122_000553"
     CDS             complement(5085..5777)
                     /locus_tag="VC122_000553"
                     /EC_number="3.1.26.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012677641.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease III"
                     /protein_id="HEP5640498.1"
                     /translation="MKQLEELLSTSFDIQFNDLTLLETAFTHTSYANEHRLLNVSHNE
                     RLEFLGDAVLQLIISEYLFAKYPKKTEGDMSKLRSMIVREESLAGFSRFCSFDAYIKL
                     GKGEEKSGGRRRDTILGDLFEAFLGALLLDKGIDAVRRFLKQVMIPQVEKGNFERVRD
                     YKTCLQEFLQTKGDVAIDYQVISEKGPAHAKQFEVSIVVNGAVLSKGLGKSKKLAEQD
                     AAKNALAQLSEV"
     gene            complement(6210..7019)
                     /locus_tag="VC122_000554"
     CDS             complement(6210..7019)
                     /locus_tag="VC122_000554"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011184291.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HEP5640499.1"
                     /translation="MNESGFKYSILASGSTGNCFYLETPKKRLLIDAGLTGKKITSLL
                     AEIDRKPEDLDAILITHEHSDHIKGVGVMARKYHLDIYANEKTWQLMDECNMLGKLDA
                     SQKHIFQRDKVLTFGDVDIESFGVSHDAIDPQFYRIMKDNKSFVMLTDTGYVSDRMTG
                     IIENADGYLIESNHDIEILRSGSYPWSLKQRILSDLGHLSNEDGAGAMIRSLGYNTKK
                     IYLGHLSKENNIKELAHMTMVNQLAMADLAVGTDFTVHDTSPDTACPLTDI"
     gene            complement(7023..8375)
                     /gene="vicK"
                     /locus_tag="VC122_000555"
     CDS             complement(7023..8375)
                     /gene="vicK"
                     /locus_tag="VC122_000555"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011017494.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell wall metabolism sensor histidine kinase
                     VicK"
                     /protein_id="HEP5640500.1"
                     /translation="MTRDIIGNLSTFELAILILLVFVAFYFIHLAVRDYRNARIIRMM
                     SHKIRDLINGRYTDIIDEKADIELMELSDQLNDLSDVFRLTHENLAQEKNRLASILAY
                     MSDGVLATDRSGKIIMINETARKQLNLSKEEALKKNITDLLEGDTSYTYRDLVSKTPV
                     VTVNSRNDMGEFVSLRLRFALNRRESGFISGLVVVLHDTTEQEKEERERRLFVSNVSH
                     ELRTPLTSVKSYLEALDEGALKEDIAPSFIKVSLDETNRMMRMISDLLNLSRIDNQVT
                     QLAVEMTNFTAFITSILNRFDLVKNQHTGTGKVYEIVRDYPITSVWIEIDNDKMTQVI
                     ENILNNAIKYSPDGGKITVRMKTTDTQLIISISDQGLGIPKTDLPLIFDRFYRVDKAR
                     SRAQGGTGLGLAIAKEIIKQHHGFIWAKSDYGKGSTFTIVLPYEKDAAIYEEWEEDVD
                     "
     gene            complement(8368..9078)
                     /locus_tag="VC122_000556"
     CDS             complement(8368..9078)
                     /locus_tag="VC122_000556"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019321978.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HEP5640501.1"
                     /translation="MKKILIVDDEKPISDIIKFNLTKEGYDIVTAFDGREAVTIFEEE
                     KPDLIILDLMLPELDGLEVAKEIRKTSHVPIIMLSAKDSEFDKVIGLEIGADDYVTKP
                     FSNRELLARVKAHLRRTETIETAVAEENASSGTQELTIGNLQILPDAFVAKKHGQEVE
                     LTHREFELLHHLANHMGQVMTREHLLEIVWGYDYFGDVRTVDVTVRRLREKIEDTPSR
                     PEYILTRRGVGYYMKSYD"
     gene            complement(9240..10274)
                     /locus_tag="VC122_000557"
     CDS             complement(9240..10274)
                     /locus_tag="VC122_000557"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002990676.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3114 domain-containing protein"
                     /protein_id="HEP5640502.1"
                     /translation="MCYNMVKKDEEKAMKQAILDRYQALKCYQNAGLSNQAFRAIAKE
                     PIIDNRLGSPTFWVIWPIEKENQSAKQLLTFLLDLVEMPFELSGQLHETQTLLTRFHP
                     SLLPDHMFWKELASLVDQAFPGKTLSQAGELEKRLHQFRYVISSQQAQSIRNHYKMIE
                     MTDAQALALFLRSKKGPCLWRQAPDYTLMDSARLHNKLRFEDNKVIFPSQEVSYNIKV
                     LLWFHTEFILDSTGFFLNEVDAEVVTEKGIVNGASFNYGTDGPRHWDLDVDPISRHDP
                     QFRRDTLKGFRSPKRVFRQWFRAQKDDFMFSYFNAKGLFAYHNKSSFARVKKSAKQFK
                     RQIHPIKGWF"
     gene            complement(10330..11577)
                     /locus_tag="VC122_000558"
     CDS             complement(10330..11577)
                     /locus_tag="VC122_000558"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002985649.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AMP-binding protein"
                     /protein_id="HEP5640503.1"
                     /translation="MLTKLEYWAKQCPNKKAIVADQISLTYQELWQAVLIKDQTIKDS
                     VPYIISHSRYLNQLLSFLRGLKEGSCPIILHPNISGTFQQQIKHVDSELLKKADFAVL
                     SSGTTGKAKLFWRCLSTWTRLFDYQNKVFGMTGNSCLFLHGSFSFTGNLNLALAQLWA
                     GGCLVLSQKLSLKTWLSLWQAKKVSHLYLLPTYLNRLLPYLTKNNMTATHLLTSSQMI
                     SQELLRHYYKKFPQLEIVIFYGASELSFITWCNGRAAVKINGLVGQPFPDVSISFKDK
                     EIFVETPYSVEGMSQPYSVSDLGKMSPAGLILEGRQDDWVNQRGVKCHLPSLVELAHQ
                     APNVKEAHALKIGKGENETLILVLVLTKKDCLAPIKDFLALYLNSGQLPKYYLVIDCL
                     PLKDNGKINREVLLNKIPKQWLS"
     gene            complement(11534..12682)
                     /locus_tag="VC122_000559"
     CDS             complement(11534..12682)
                     /locus_tag="VC122_000559"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009880280.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA C-acyltransferase"
                     /protein_id="HEP5640504.1"
                     /translation="MTDVYIAAGLRTPIGLVGKQFAKEQPEILGAKLINALQNKYPVP
                     IDQVICGNAVGTGGNIGRLMTLYSHLGESVSALTVDMQCASAGAALSVGYAKIKAGMA
                     SNLLVGGIESSSLQPESVYASADWRQGAYKVAQFSPDSISPFAMIEGAERVAREHGFT
                     KEYLNHWTLRSHQKASYCQEQALLADLILDLPGASDQGIRPRLSSKVLSKVPPILGED
                     HVISAANACLTHDAAAFLQLSSQPSAFKLIDVVEVAGDPQRSPLMVIKASQVLLEKHG
                     LGMADMTAIEWNEAFAVIDGLFETHYPDLLDRYNIFGGALAYGHPYGASAAIIILHLM
                     RALEIKNGRYGIAAIAAAGGQGFAVLLKYHKEFSHADKARILGKAMPK"
     gene            complement(13077..13226)
                     /locus_tag="VC122_000560"
     CDS             complement(13077..13226)
                     /locus_tag="VC122_000560"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002990686.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroxyacetone kinase"
                     /protein_id="HEP5640505.1"
                     /translation="MQLLDNVYGTLRSFAFMRPVKSSNDTSLADALQADTDMAFILVR
                     GINYQ"
     gene            complement(13312..14097)
                     /locus_tag="VC122_000561"
     CDS             complement(13312..14097)
                     /locus_tag="VC122_000561"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003010202.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEP5640506.1"
                     /translation="MAKLRRMHAIFIKQYLKQIMEYKVDFVVGVLGVFLTQGLNLLFL
                     SVLFQHIPSLEGWTFEQIAFIYGFSLIPKGIDHLFFDNLWALGQRLVRKGEFDKYLTR
                     PISPLFHVLVETFQVDALGELLVGVILLVTTAGSIVWTLPKVLLFILVIPFATLIYTS
                     LKIATASISFWTKQSGAVIYIFYMFNDFSKYPMSIYHSFLRWLISFVIPFAFTAYYPA
                     SYFLTGQHLLFNIGGLVVVSLLVLALSLKLWKWGLDAYESAGS"
     gene            complement(14099..14917)
                     /locus_tag="VC122_000562"
     CDS             complement(14099..14917)
                     /locus_tag="VC122_000562"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014407390.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC-2 family transporter protein"
                     /protein_id="HEP5640507.1"
                     /translation="MWSFWKRYRPFLSAGIQELITYRVNFFLYRIGDVMGAFVAYYLW
                     KAVFDSSKQSLINGFTLSDMTFYIIMSFVTTLLTKSDSSFMIGEEVKDGSIIMRLLRP
                     VHFAASYLFMEIGFRWIVLMSVGFPFLMVLSGIKVMAGLSTLQVLASSCLYLVSLLLA
                     FLINFYFNICFGFSAFVFKNLWGSNLLKNALVAFMSGSLIPLAFFPKVVYIVLSFLPF
                     SSLVYTPVMIVIGKYSLSQIMVALSLQIFWLLVMVVLSQVIWKKVQYHLTIQGG"
     gene            complement(14919..15911)
                     /locus_tag="VC122_000563"
     CDS             complement(14919..15911)
                     /locus_tag="VC122_000563"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018371202.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HEP5640508.1"
                     /translation="MVMIEVSHLQKNFSKTIKEPGLKGALKSFVHPQREIFEAVKDLS
                     FEVPKGQILGFIGANGAGKSTTIKMLTGILKPTSGYCRINGKIPQDNRQDYVRDIGAV
                     FGQRTQLWWDLALQETYVVLKEIYDVPEKAFRKRMDFLNEVLDLNEFIKDPVRTLSLG
                     QRMRADIAASLLHNPKVLFLDEPTIGLDVSVKDNIRRAITQINQEEKTTILLTTHDLS
                     DIEQLCDRIIMIDKGQEIFDGTVTQLKQSFGKMKSLSFELKPGQEQVVSQFMGLPDIT
                     VERHELSLDIQYDSSRYQTADIIQKTMADFAVRDLKMTDVDIEDIVRRFYRKEL"
     gene            complement(16052..17995)
                     /gene="thrS"
                     /locus_tag="VC122_000564"
     CDS             complement(16052..17995)
                     /gene="thrS"
                     /locus_tag="VC122_000564"
                     /EC_number="6.1.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003061393.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="threonine--tRNA ligase"
                     /protein_id="HEP5640509.1"
                     /translation="MIKITFPDGAVREFESGVTTFDIAESISKSLAKKALAGKFNDQL
                     IDTTRAIEEDGSIEIVTPDHKDAYEVLRHSAAHLFAQAAKRLFPNLHLGVGPAIAEGF
                     YYDTDNAEGQISNEDLPRIEAEMQKIVTENYPCIREEVTKEEALELFKDDPYKVELIN
                     EHAGAGLTVYRQGEFVDLCRGPHVPSTGRIQVFHLLNVAGAYWRGNSDNNMMQRIYGT
                     AWFDKKDLKAYLTRLEEAKERDHRKLGKELDLFMISQEVGQGLPFWLPDGATIRRTLE
                     RYITDKELASGYQHVYTPPLASVELYKTSGHWDHYQEDMFPVMDMGDGEEFVLRPMNC
                     PHHIQVYKNHVRSYRELPIRIAELGMMHRYEKSGALSGLQRVREMTLNDGHIFVTPEQ
                     IQEEFQRALQLIIDVYADFNLTDYRFRLSYRDPNDTRKYYDNDEMWENAQSMLKAALD
                     EMGVDYFEAEGEAAFYGPKLDIQVKTALGNEETLSTIQLDFLLPERFDLKYIGADGEE
                     HRPVMIHRGVISTMERFTAILIETYKGAFPTWLAPHQVTVIPISNEAHIDYAWEVAKT
                     LRDRGVRADVDDRNEKMQYKIRASQTSKIPYQLIVGDKEMEDKSVNVRRYGSKATHTE
                     SVEEFVENILADIARKSCPDAQA"
     gene            complement(18417..19751)
                     /locus_tag="VC122_000565"
     CDS             complement(18417..19751)
                     /locus_tag="VC122_000565"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011184276.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
                     /protein_id="HEP5640510.1"
                     /translation="MRVGLFTDTYFPQVSGVATSIRTLKEELEKEGHEVYIFTTTDRD
                     VKRFEDPTIIRLPSVPFVSFTDRRVVYRGLISSYKIAKHYNLDIIHTQTEFSLGLLGK
                     MIGKALRIPVVHTYHTQYEDYVSYIANGKIIRPSMVKPLLRGYLKDLDGVICPSRIVL
                     NLLEGYEVTIPKRVIPTGIPLEKYIRDDITAEEVTNLKAELGIAGDETMLLSLSRISY
                     EKNIQAIINQMPAILAENAKIKLIIVGDGPYLQDLKHLAMQLEVDKHVTFTGMVPHDK
                     VALYYKACDFFISASTSETQGLTYIESLASGTPIIAHGNPYLDDVVTDKMFGTLYYAE
                     TDLTDAIIDAILKTPVMDKRLLAKKRYEISAQHFGKSIYTFYLDTLIARNSKEAQKLS
                     LYLNHSGKSSSLKLVQGAIHLPKRAAKVTAITSVKVVKAPIKLVHAIKDFLD"
     gene            complement(19753..20751)
                     /locus_tag="VC122_000566"
     CDS             complement(19753..20751)
                     /locus_tag="VC122_000566"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011017479.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
                     /protein_id="HEP5640511.1"
                     /translation="MKVLLYLEAENYLRKSGIGRAIKHQAKALSLVGQHFTTNPRETY
                     DLVHLNTYGLKSWLLMIKAQKAGKKVIMHGHSTEEDFRNSFIFSNLLSPWFKKYLCHF
                     YNKADAIITPTLYSKSLIESYGVKSPIFAVSNGIDLEQYGADSKKEAAFRRYFDIKEG
                     EKVVMGAGLFFLRKGIDDFVKVAQAMPDVRFIWFGETNKWVIPAQVRQMVNGNHPKNL
                     IFPGYIKGDVYEGAMTGADAFFFPSREETEGIVVLEALASRQHLVLRDIPVYYGWVDQ
                     SSAELATDIPGFIEALKKVFSGASNKVEAGYKVAQSRRLETVGHALVDVYKKVMEL"
     gene            complement(20882..21883)
                     /gene="ccpA"
                     /locus_tag="VC122_000567"
     CDS             complement(20882..21883)
                     /gene="ccpA"
                     /locus_tag="VC122_000567"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002264971.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="catabolite control protein A"
                     /protein_id="HEP5640512.1"
                     /translation="MNTDDTITIYDVAREAGVSMATVSRVVNGNKNVKENTRKKVLEV
                     IDRLDYRPNAVARGLASKKTTTVGVVIPNIANSYFSILAKGIDDIAAMYKYNIVLASS
                     DEDDDKEVNVVNTLFAKQVDGIIFMGHHLTEKIRAEFSRSRTPVVLAGTVDLDHQLPS
                     VNIDYRAAVSNVVDILAENHKCIAFVSGPLIDDINGKVRLAGYKEGLKHNKLDFKEGL
                     VFEANYSYKEGFELAQRVINSGATAAYVAEDELAAGLLNGLFEAGKRVPEDFEIITSN
                     DSPVVQYTRPNLSSISQPVYDLGAVSMRMLTKIMNKEELEEKEILLNHGIKKRGTTK"
     gene            22057..23142
                     /locus_tag="VC122_000568"
     CDS             22057..23142
                     /locus_tag="VC122_000568"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011054264.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminopeptidase P family protein"
                     /protein_id="HEP5640513.1"
                     /translation="MTKLDQIRLYLDQKGAELAIFSDPVTINYLTGFFCDPHERQLFL
                     FVYHDLAPVLFVPALEVARASQAISFPVFGYVDSENPWEKIKAVLPNTAAKTIYVEFD
                     HLNVNKFHGLQTIFSGQFNNLTPYVQGMRLVKSADEINKMMIAGQFADKAVQVGFDNI
                     SLDATETDVIAQIEFEMKKQGIHKMSFDTMVLTGNNAANPHGIPGTNNIENNALLLFD
                     LGVETLGYTSDMTRTVAVGQPDQFKIDIYNLCLEAQLAAIDFIKPGVTAAQVDAAARQ
                     VIEKAGYGEYFNHRLGHGIGMDVHEFPSIMAGNDLVLEEGMCFSVEPGIYIPGKVGVR
                     IEDCGHVTKNGFEVFTHTPKELLYFEG"
     gene            23191..23856
                     /locus_tag="VC122_000569"
     CDS             23191..23856
                     /locus_tag="VC122_000569"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002985684.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-dependent oxidoreductase"
                     /protein_id="HEP5640514.1"
                     /translation="MDQTIHHQIQQALHFRTAVRVYKEEKISDEDLALILDAAWLSPS
                     SVGLEGWRFVVLDNKPIKEEIKPFAWGAQYQLETASHFILLIAEKHARYDSPAIKNSL
                     LRRGIKEGDGLNSRLKLYESFQKEDMDMADNPRALFDWTAKQTYIALGNMMMTAALLG
                     IDTCPIEGFHYDKVNHILAKHNVIDLEKEGIASMLSLGYRLRDPKHAQVRKPKEEVIS
                     VVK"
     gene            23867..24244
                     /locus_tag="VC122_000570"
     CDS             23867..24244
                     /locus_tag="VC122_000570"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002985686.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HEP5640515.1"
                     /translation="MKALHTCIRVKDLDQSVAFYTSAFPFKENYRKDFPDSQFTLVYL
                     ALEGESYELELTYNYGHGDYDLGNGYGHIALGSEHFEADHKKHRQAGFPVTDIKELAD
                     KSARYYFIQDPDGYKIEVIDLNN"
     gene            24389..25315
                     /locus_tag="VC122_000571"
     CDS             24389..25315
                     /locus_tag="VC122_000571"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011106859.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HEP5640516.1"
                     /translation="MTLLSIIVPCFNEEANILPYFEEMHQLETSMTNQLAFEYIFIDD
                     GSKDNTLGILRELAARFPNVHYLSFSRHFGKEAGLLAGLKEAKGNYITVMDVDLQDPP
                     ELLPVMYAKLKEGYDIVGTRRQNRQGEPLIRSMCSNLFYGLIKHLSDTEMVNGVRDYR
                     LMTRQVVDSILELGEVNRFSKGIFSWVGYRITYLSFENQKRKYGKSRWHFWELLRYSL
                     DGFINFSEMPLTIATWTGTFSFLISIFAILFIIIRKILFGDPVSGWASTVSIILFMGG
                     IQLFCMGIIGKYISKIFLETKKRPLYIIKEKH"
     gene            25526..26209
                     /locus_tag="VC122_000572"
     CDS             25526..26209
                     /locus_tag="VC122_000572"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002994173.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF969 domain-containing protein"
                     /protein_id="HEP5640517.1"
                     /translation="MEWIKLIGIVIIVLGFILKFDAIATVVVAGLVTALVSGISFIDF
                     LDILGKEFTNQRLLTIFFITLPLIGLSETYGLKHRATQLIQRVQALTVGRLLTLYLII
                     RELAGLFSIRLGGHPQFVRPLIQPMGEAAAKANIGEELTDAEKDDIKAMAAANENFGN
                     FFAQNTFVGAGGVLLIAGTLEQLGYDGNQAKIAFSSILIAIISIIIVAIYNYLFEKKM
                     ERQHQKGDN"
     gene            26209..27135
                     /locus_tag="VC122_000573"
     CDS             26209..27135
                     /locus_tag="VC122_000573"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011284586.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF979 domain-containing protein"
                     /protein_id="HEP5640518.1"
                     /translation="MANIANLVLETIYILIGLQLFHTAYCTFKDKTNPVYFGTALFWG
                     LLGVTFVGGAFLPNKVIGFIVIVLALLTLFKQVRIGTLPAFNEQKAEESAHRIGNWIF
                     LPVMLMAMISLLLALILPDFSKSAIGIAGILATIAILIITKQKPSALLAENNRMNQQV
                     STSGILPQLLGALGAIFAAAGVGDVIASLIREIVPADSRFFGVLAYVLGMVIFTMIMG
                     NAFAAFTVITTGIGVPFVFALGADPIIAGALAMTAGFCGTLLTPMAANFNALPVALME
                     IKDRNAVIKKQAPIALVLIISHIALMYLLAYY"
     gene            27185..27832
                     /gene="pcp"
                     /locus_tag="VC122_000574"
     CDS             27185..27832
                     /gene="pcp"
                     /locus_tag="VC122_000574"
                     /EC_number="3.4.19.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002990717.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyroglutamyl-peptidase I"
                     /protein_id="HEP5640519.1"
                     /translation="MKILVTGFDPFGGEAINPALEAIKKLPATIHGAEIKCIEVPTVF
                     QKSADVLQQHIESFQPDAVLCIGQAGGRTGLTPERVAINQDDARIPDNEGNQPIDTPI
                     RADGKAAYFSTLPIKAMVAAIHQAGLPASVSNTAGTFVCNHLMYQALYLVDKYCPNAK
                     AGFMHIPFMMEQVVDKPNTAAMNLDDITRGIEAAIFAIVDFKDRSDLKRVGGATH"
     gene            complement(27949..28668)
                     /locus_tag="VC122_000575"
     CDS             complement(27949..28668)
                     /locus_tag="VC122_000575"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011106860.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutaminyl-peptide cyclotransferase"
                     /protein_id="HEP5640520.1"
                     /translation="MSIMITKGQVRLLRTYSYDSNLYTQGLEQLNNNHILLSAGRYGF
                     SKVGVYDLTQEIFSEKIAFPDTVFAEGLTVVEDYFWLLTYKEGVAYKFDKATCNCLGA
                     YPFEGDGWGLAYDKENQCLWMTSGNAFLQKRDPKDFALLDTVLVAIESVPISMLNELE
                     YVDGYLYANIWQTNTIVKLQPDSGKVVATYDISPLLKALNLDKSHYPDLNVLNGIAHL
                     DQQRFLITGKLYPLMLEVVLD"
     gene            complement(28674..29141)
                     /gene="smpB"
                     /locus_tag="VC122_000576"
     CDS             complement(28674..29141)
                     /gene="smpB"
                     /locus_tag="VC122_000576"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002994152.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SsrA-binding protein SmpB"
                     /protein_id="HEP5640521.1"
                     /translation="MAKGEGHILAQNKKARHDYHIVETVEAGIVLTGTEIKSVRAARI
                     QLKDGFAQIKNGEAWLVNVHIAPFEQGNIWNADPERTRKLLLKKREITHLANELKGSG
                     MTLVPLKVYLKDGFAKVLIGLAKGKHEYDKRETIKRRDQERDIKKQMKHYNAR"
     gene            complement(29144..31477)
                     /gene="rnr"
                     /locus_tag="VC122_000577"
     CDS             complement(29144..31477)
                     /gene="rnr"
                     /locus_tag="VC122_000577"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010921980.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease R"
                     /protein_id="HEP5640522.1"
                     /translation="MMKERIMTYLKEHGKSNVNDLAAALEMAGAKHFPSLIKTISKME
                     SQSLLRFSDDGSLALRKEREKKKEPTVQGVFRANKAGFGFLHVDENEDDMFIGRNDVG
                     YAIDGDTVEVVVKKPADRLKGTAAEAKVVAIVDRSLKTAVGTFILDDDKPKYAGYIRS
                     KNQKIQQKIYIKKEPVVLKGTEIIKVDIDKYPTRGHDYFVASVRDIVGHQGDVGIDVL
                     EVLESMDIVSEFPAEVLAEANAISEAPTAKDLIGRVDLRQETTITIDGADAKDLDDAI
                     HIKLLDNGNYELGVHIADVSYYVTEGSALDKEAIARGTSVYVTDRVVPMLPERLSNGI
                     CSLNPNVDRLTQSALMEINSQGHVVNYQICQSVIKTTYRMTYSTVNDMIAGDEEALQE
                     FASIADDVTLMVALHRILEAMRSKRGALNFDTQEAKVIVNDKGMPVDVVLRQRGIAER
                     MIESFMLAANECVAEHFAKAKLPFIYRIHEEPKAEKLQQFIDYASTFGIHIQGTANKI
                     SQEALQAFMAKVEGQPGAEVLNMMLLRSMQQARYSEHNHGHYGLAAEYYTHFTSPIRR
                     YPDLLVHRMVREYSQPSQEKRDHFTQIIPELATSSSQLERRAIDAERVVEAMKKAEYM
                     AEYVGEEFDGIVASVVKFGFFVELPNTIEGLVHITSLPEYYHFNERTLSLQGEKSGKV
                     FKVGQPIRVKLVKADKETGDIDFEYLPSDFDVVEKIKMSDKASRRDRRKSSKSSKGTK
                     KKEPKEVAKAKTKGKTKKGSKKPFYKEQAKKKSRKRS"
     gene            complement(31571..31807)
                     /gene="secG"
                     /locus_tag="VC122_000578"
     CDS             complement(31571..31807)
                     /gene="secG"
                     /locus_tag="VC122_000578"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018374825.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecG"
                     /protein_id="HEP5640523.1"
                     /translation="MYNLLLTILLVLSVLLVIAIFMQPQKNPSSNVFDSSGSEALFER
                     TKARGFEAFMQRFTAVLVFFWLAIALVLAILSSK"
     gene            complement(31853..31999)
                     /gene="rpmG"
                     /locus_tag="VC122_000579"
     CDS             complement(31853..31999)
                     /gene="rpmG"
                     /locus_tag="VC122_000579"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019803498.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L33"
                     /protein_id="HEP5640524.1"
                     /translation="MRVKINLECSECGSNNYLTSKNKSSHPEKIKVPKYCPKERKVTL
                     HVET"
     gene            complement(31996..33189)
                     /locus_tag="VC122_000580"
     CDS             complement(31996..33189)
                     /locus_tag="VC122_000580"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002990732.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux MFS transporter"
                     /protein_id="HEP5640525.1"
                     /translation="MNWRQNLKVAWLGNFFTGASFSLVMPFMALYVENLGTPKELVEY
                     YAGLAVAVTALASALFAPVWGKLADRYGRKPMMLRASFVMTFTMGGLAIIPNVFWLLI
                     LRLLTGVFAGYVPNATALIASQAPKEESGYALGTLATGVTAGALIGPLLGGILAELLG
                     IRQVFLLVGVILFLCSLMTAVYVKEEFKPIRRFEMIPTKVILKQVKSPQIMLGLFVTS
                     MIIQISAQSVAPILSLYIRHLGQTHNLMFTSGLVVSAMGFSSLFSSSYLGKLGDRFGN
                     HRLLLAALCYSFIMYFSSALAQTSFQLGVLRFAYGFGVGALMPSINSLLTKLTPKEGI
                     SRVFAYNQMFSNLGQVIGPFIGSNVAVVLGYRSVFYVTSLIVFVNLIWSLIIFRKYIK
                     VKDIV"
     gene            complement(33311..34813)
                     /locus_tag="VC122_000581"
     CDS             complement(33311..34813)
                     /locus_tag="VC122_000581"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012560520.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF853 domain-containing protein"
                     /protein_id="HEP5640526.1"
                     /translation="MTTMTIAKGSQPIALQLGMLNRHGLIAGATGTGKTVTLKVLAEQ
                     LSLAGVPVFLADIKGDLSNLTKAGEVTDKLAARLATIGVSDYQPQAFPVRMWDVFGQN
                     GQPLRTTISEMGPMMLSRLLNLNDTQTGVLNIVFKIADEKGWLLIDLKDLQAILKEVG
                     DHASDYSSHYGNIAKQSIGAIQRSLLTLEQEGAHQFFGEPALDVADLMQLDVASGYGA
                     INILSATKLFQSPTLYTTFLLWLLSELYELLPEVGDLDKPKMVFFFDEAHLLFKDAPK
                     VFLEKVEQIVRLIRSKGVGIFFVIQNPLDLPETVLAQLGNRIQHALRAYTPKEQKAVR
                     VAADTFRQNPDLDVARVITELEVGEALISVLNDKGQPSIVERAYIMPPKSSFAVLSEI
                     ESQQLVQSSPFASKYSQSIDRESAYEKLAAKVLEDNRLAQEAIATAKREKEAKEAIKA
                     QAATKKASRRSVGRPRKTVVEKATDAFISTTVRTIGRELVRGLLGSLRKR"
     gene            complement(35003..35596)
                     /locus_tag="VC122_000582"
     CDS             complement(35003..35596)
                     /locus_tag="VC122_000582"
                     /EC_number="2.7.1.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011017469.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dephospho-CoA kinase"
                     /protein_id="HEP5640527.1"
                     /translation="MIIGITGGIASGKSTVVKVIRKAGYQVIDADQVVHDLQEKGGRL
                     YEALREAFGNQILKADGELDRTKLSEMLFSNPDNMATSSAIQNQIIKEELAAKRDHLA
                     QSQAIFFMDIPILMELGYQDWFDAIWLVYVDAQTQLQRLMARNRLDKGKARQRIASQL
                     PIEEKKPYASLVIDNSGDIAALIKQVQSALLSLANPR"
     gene            complement(35593..36420)
                     /gene="mutM"
                     /locus_tag="VC122_000583"
     CDS             complement(35593..36420)
                     /gene="mutM"
                     /locus_tag="VC122_000583"
                     /EC_number="3.2.2.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011528354.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-formamidopyrimidine glycosylase"
                     /protein_id="HEP5640528.1"
                     /translation="MPELPEVETVRRGLETLVLGQEIVAVTLKVPKMVKTDLETFALT
                     LPGQIIQSVGRRGKYLLIDLGQLVLVSHLRMEGKYLLFPDEVPDNKHFHVFFELKNGS
                     TLVYQDVRKFGTFDLIAKSQLSAFFAKRKLGPEPKKETFKLKTFEAALLSSQKPIKPH
                     LLDQTLVAGLGNIYVDEVLWAAKVHPGTASSRLNKAEIKRLHDETIRILALGIEKGGS
                     TVRTYRNALGADGTMQDYLQVYGQTGKPCPRCGQAIVKLKVGGRGTHICPKCQKKRP"
     gene            complement(36592..37458)
                     /gene="rgg2"
                     /locus_tag="VC122_000584"
     CDS             complement(36592..37458)
                     /gene="rgg2"
                     /locus_tag="VC122_000584"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011889095.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="quorum-sensing system transcriptional regulator
                     Rgg2"
                     /protein_id="HEP5640529.1"
                     /translation="MEKELGKTLRRLRKGKQVSISFLADEYLSKSQISRFERGESEIT
                     CSRLLNLLDKLNITIDEFVSAHSKTHTHFFTLLSQARKCYAEKNVVKLTKLLKDYAHK
                     DYERTMIKAILFSIDSSIAPSQEELTRLTDYLFKVEQWGYYEIILLGNCSRFMNYNTL
                     FLLTKEMVASFAYSEQNKTNKMLVTQLSINCLIISIDHSCFEHSRYLINKIDLLLRDE
                     LNFYEKTVFLYVHGYYKLKQEEMSGEEDMRQALQIFKYLGEDSLYYSYKEHYRQIVLG
                     GKGDEDWSEADL"
     gene            37547..37615
                     /gene="shp2"
                     /locus_tag="VC122_000585"
     CDS             37547..37615
                     /gene="shp2"
                     /locus_tag="VC122_000585"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_128650799.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide pheromone SHP2"
                     /protein_id="HEP5640530.1"
                     /translation="MKKVNKALLFTLIMDILIIVGG"
     gene            38529..38798
                     /gene="stcA"
                     /locus_tag="VC122_000586"
     CDS             38529..38798
                     /gene="stcA"
                     /locus_tag="VC122_000586"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012560518.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="quorum-sensing system protein StcA"
                     /protein_id="HEP5640531.1"
                     /translation="MTKKQFYLILAISILCFILAVSPKPPIPKHTIERKRTAMTIGHS
                     KQTPHKKEALKHKKNKNKALFLPSGDSAIKSVISMTVRLLLDDQY"
     gene            complement(39146..39544)
                     /locus_tag="VC122_000587"
     CDS             complement(39146..39544)
                     /locus_tag="VC122_000587"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014635342.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine kinase"
                     /protein_id="HEP5640532.1"
                     /translation="MHRLPNRLIPEEDNNKLSRVAKEVVTEPRLNNPFLLPKKVLSLE
                     DDAAILNHLEEDRNVNKLFLEPVAAEAKREINFHPSDQQVTAKVKESSDFHQKQHQTG
                     ITYGDDTLADLTHHLLHSDGVIPSLNDKSA"
     gene            complement(39641..40711)
                     /locus_tag="VC122_000588"
     CDS             complement(39641..40711)
                     /locus_tag="VC122_000588"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011528351.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEP5640533.1"
                     /translation="MMMLRDVKVKMSSLLVGCAATLLVSLSSTVAADSVHSSDRRFGV
                     GYKEETTSQFSSDQPSQVVLYRGEANTVRFTYTNQMYLMKDIRIALDGSGKSLTAQTV
                     PGMGHVYEGFQTSARGIFTMSGVPESTVPVANPNVQTKYTRYFKVIDDMHNTMYKGTV
                     FLVQPQAWKYTMKSVDQLPVDDLNHIGVAGIERMTTLIKNAGALLTTGSSGAFPDNIK
                     VSINPRGGQATIIYGDGSTDIIPSAVLWEKGSVKEPTEADQSVGTPTPGIPGKFKRDQ
                     SLNEHEAMVNVEPLSHVVKDNIKVIDEKSTGRFEPFRPNEDEKEKPASDVKVRPAEVG
                     SWLEPATALPSVEMSAEDRLKS"
BASE COUNT        12332 a         8725 c         7177 g        12734 t
ORIGIN      
        1 tttggatcat ttatcagatg tttgatttta tcttctaaac acactgagtc taccaaaatt
       61 tttttatgtt tatattcatc cagaactcct atgttgtgat ttagatattc aaataattca
      121 tctgatatag aatgttcaac tggattacca agcaatgcag aatacataaa tttcatcctc
      181 ctacgtttaa ttagtttact actaacataa agtacaagca ttacacagag ctaaccatta
      241 cctataagtc tttagagtat ctaaagactt aggtgtacac ctaagtaact tacccaccaa
      301 caataatgat aatatccatt gctaaaatta aaatcggcaa aaattttgaa attttcttca
      361 taagcggact tcctttcagt ttttatttat tgtacaataa aaaaaaacgg aattgatttt
      421 tgttgggaaa gtgggaaaat atgaaatcaa aacttggtgc cacacttaga aaagttcgaa
      481 aaggaaagca aattagtctg tgcttcgtgg cagatgaaca cctttcaaaa tcacaaatct
      541 ctcgttttga gcgcggggag tcagagatat cgtgtatccg actgatcaat attctagaca
      601 aattacacat tactttggat gagtttctta ttttacatga tgaagactat accaattccg
      661 aaacatttgc taacctggta cagtatattc gtaagcaata ttcatcacaa aatatcaata
      721 atatagcatg tttactttcc gacacttctg attacactct gaattctttt gagaaaacaa
      781 tggtaaagtc cattttacac acaatggatt caagcatcat tccatcagat gatgaattac
      841 ttcaactaac agattatctc tttaaagttg aaaaatgggg ctactatgaa atccttttgt
      901 tgggtaactg tgtacgaaca attaactaca actcctactt cctattgaca aaggagatgt
      961 taaataatta catttactct tcacggaaca aaactaataa acgaattgtc actcagcttg
     1021 ctataaattg tttcattctt agtatcaata aggaggaatt ttctaattgc tcctatttaa
     1081 ttgatgagat aaaatcatta ttagataatg aattaaactt ctatgaacag acagtttttc
     1141 tctatgcaac aggttacttt gaatttaagc gccaattaga aactggagtt gaaaagatga
     1201 aacaagccat acaagttctt gatattttaa atgaagacaa gttaaaatta cattacgctg
     1261 accattttga taaactagtc aacaaagatt aagtgaaatc attctttacc ttttaacgaa
     1321 atgccaatca agagtgtctc aataaaaaat catctactct gacaactttt ctcttttacc
     1381 tatcgacagc taaaaagcct tggaaactgc tatttccaag gctttctttg tactctattg
     1441 tatcaattag cttattccga tttctgttgc tattagttta atcatgaaat ctcctaactt
     1501 atgaccgaac atccaaaaaa acagaaggaa ctcccttctg ttttttaatt ggtcatctct
     1561 tgagcttctt ttaattttac agatacaatt ttagagaccc ccgattcctg catggtaatt
     1621 ccataaatac tatctgctgc agccattgtt cctttacgat gggtcacgac aataaattga
     1681 ctatctttgt caaagcgatt gaggaaatcc ccaaagcgct taacatttgc ttcatcaaga
     1741 gccgcttcta cctcatctaa aatcacaaac ggaatcgttt taacacgtat aatagcaaac
     1801 aataaagcca gtgctgacaa agctttctct ccgcctgaca tcaagttaag agattggatt
     1861 ttcttaccag gaggctgcac agaaatctct ataccagcag acaagagatc tgtttccgtt
     1921 aaaaccaaat cagcagaacc cccaccaaac atctgagtaa aggtttcctt aaaacttttt
     1981 cgaattgcct caaaagtcac tttgaaacga gccttcactt cgctatccat actattaata
     2041 gtctctagca aaagattttt agcttttgtc agatctgtct tttgacttgt taaaaaagtc
     2101 aaacgctcat gcacttcctc atactgatta atagcatcac tattaattgg tcctagtgca
     2161 cgaatagtct tttgtaaatg gtgtaatttt tctttggcac tttctaagtg atcaatacta
     2221 tttgttacct cctttgcgtc tgctagtgtc atttgaaaat cttctgataa acttttagca
     2281 taggctcgta agcgattagc cacttgctct aaatgtgtct ctaacttggt ttgacgacga
     2341 ataaatgtgt catttttttc gctttctttg gttatttttt ctgctgtttc ttctaagcgg
     2401 gcctcatagt cctctatttc aaatcttaat tggactaaac gtttttgagc accagactta
     2461 tgctctgtgg catcttgtaa ctgtttttgc cactggggta gtctttgaat gctgtcttga
     2521 cttacgttgt tgtttagtat gctttcaagt ttgagaatat tttgctggca ttgcttcagc
     2581 tgagttctta gacgagattg attagcttgc tcaaatttct tttcattaag taaatctctt
     2641 tcttgcaaac gagcttgaga taaagcttgg tgaatgtttt gtgttttttg cctaaccaaa
     2701 tctttgtttt ctttgatatc atcaatatca caagttaagg cacttttttt cttagcaata
     2761 gctgttaatg cttcttcaat acgagcttgc tctgccaata aagattcatc agaatgagtc
     2821 gtctgacttt gatcaagggc agctaacaaa gcctttgaat cttcttgttt ttcttgcaat
     2881 tgctgataag ccatttgagc tctttgttca gctaagcgtg cctggtcacc ggctaatttc
     2941 aattgtgtca gttcttcttt ttttgctatc aaatcgcttt gcaaggcagc tacttctttt
     3001 tcagttattt ttaattgctc aactaatcgc gttaattcct cactgatttg ttctaactct
     3061 ggtttgataa aagttgtatt gctttgtcta ttagttcctc ctgaaaaaga accacctggt
     3121 cgtaattctg ttccatcaag agtaacaatt cttaccttat atcctaataa gcgcgctgca
     3181 atattagctt gatcaatagt ttcaaagata gcagtactag ataaaaggtt ttggataata
     3241 gctgaaagac tatcatcata gcgaaccaat gattctgcag ttcccagata accttcacaa
     3301 gtcgctaatt gacgatgata gtgttctgat aatgatctag cctttatagt agttaaaggt
     3361 aaaaaagttg ctcgtccttg gcgatttttt ttcaaataag caattgctct ttttgctgca
     3421 gcttcgtctg ttacaataat atgctgacta ttagcaccta atgcaatttc aagagctgtt
     3481 tggtaatcag aatcaaaaga aaggtgttca ctgactgcac ctatgatccc tccaagcttt
     3541 ttttgtgatt gaaggacagc tcgtactccc gcatagaatt gactatgact tttttgaata
     3601 gattctaagc tagccttacg agcttctttt cctttttttt gatccaacaa gtcaaatagt
     3661 ctttcttgct tcagttggta atctctttct aattcctgaa cgcgtttatc accttcttga
     3721 taattttgga gcaacatttc gacttgctct ttttgagctt tataatgagc ttgagctttt
     3781 tgagactcgt cattaagttg atcaagctta gtaacaagca gctgatattc ttgagcctta
     3841 tgttgacgag cctgtttttc tttgtctaaa tgggctttta aagctgtcag ctgatttgat
     3901 aaggccgcct ctttttgcat caataataca aactcttctc ttagagtttc cattaattgg
     3961 tcgggatcac ttgaaaaacg ctctaatgca ttgctcaatt cgtttaactg ttgtttgaca
     4021 tcacaaagtt gttgatcaat atgcaataat tgttctgtac gctgcttttc ttctgcttga
     4081 aaaccatcca attgttcttg taactgttcg aggtgttttt tggcttctgc ctttttctct
     4141 gcttcttggc cactttctag cttaaccaat tcaatttgct tttccaagtc agctattagc
     4201 ttggttaatt ctaataatgt tgtttgggtt tgatctgact cttgactaag aacctgcttt
     4261 ttttgcttga atttttggta atcttcttcc atagattgac gcttagcgta ataagatgct
     4321 aagtcttgtt gtaaagcagc taaagcttca gtatctttag tctgtctttc ttgagctata
     4381 tcaatatctt tgactagaat atctagttgt agttgcttac gattggcatc aagttctaaa
     4441 aattgtttag ctactttagc ttgcttttca agtggggcca actgcgtgtc tagctcataa
     4501 ataatatcct ctagacggtc taaattatct tgagtttgat ttaatttaat ctgagtttct
     4561 tttttacgtg ttttatattt taaaacacca gctgcttctt caaaaatggc gcgcctttct
     4621 tcaggtttac tattgaaaat ttcctctacg cgaccttgag aaataatgga gaaagaatca
     4681 cgaccaagcc ctgtatccat aaacaaatca tgaatatcac ggagccttac cttgcgacca
     4741 tcaatcagat agtcactatc tccatttcgg taaatatgcc tttcgacacg gatttctttc
     4801 ttggcagttt ttataaaatg atcggaatta tcaagaacaa ccgctacctt agcatagttt
     4861 agaggattac ggttttgtgt cccagcaaaa atgacatctg gcatcttgcc cccacgcaga
     4921 tttttagcac ttgattctcc taaagcccaa cgtaaacttt ctgtaatatt acttttacct
     4981 gaaccatttg gtcctacgac tgcagttact cccttataaa attcaatttt agtcttgtcc
     5041 gcaaaggact tgaacccttc caactcaatt tcttttaaaa acatctaaac ctcactaagt
     5101 tgggcaaggg catttttagc agcatcttgc tccgctaatt ttttagactt acctaaacct
     5161 ttactaagga cagcaccatt aacaactatc gatacctcaa attgtttagc gtgggcaggt
     5221 cctttctcac taatgacttg ataatcaata gccacatcac cttttgtttg caaaaactct
     5281 tgaagacaag ttttatagtc tctaaccctt tcaaaattcc ctttttccac ctgagggatc
     5341 atgacttgtt tcaagaagcg acgtactgca tcaatcccct tatctaataa aagagcacct
     5401 aaaaaggctt caaataagtc tcctaaaata gtatcacgac ggcgaccacc tgacttttcc
     5461 tcaccttttc ctagtttgat ataagcgtca aaagaacaaa atcgtgaaaa acctgccaag
     5521 ctttcctcac gtacaatcat ggaacgtaac ttagacatat ctccttctgt ctttttagga
     5581 tactttgcaa ataaatactc tgaaataatc aattgtagaa cagcgtctcc taaaaattcc
     5641 aagcgttcat tatgtgaaac gtttaggaga cgatgctcgt tagcgtaaga ggtgtgggta
     5701 aaagctgttt ctagtaaagt caaatcatta aattgaatat caaaagatgt tgagagtaac
     5761 tcttcaagct gtttcataaa aaatcccttt catatttgtc agtagacctt atttagtata
     5821 ccaaaaatcc acctagaaaa ccgactctaa gtggattttt cgtgatttat taaacatctt
     5881 aaatacccgt caaaggaaac tttctcatac aatcaaaaga ctctacacta tctgagggga
     5941 atttcccact acagatagta tagagctatt ttgtttttag tctgaacaac taaaaaacta
     6001 ccttatcttt ataatcctca acacctttga taaatagatc agacaagcca aaactacagg
     6061 ctaatttaag tctattgatg ctctaaagga tgaccaatga gagttcattg aactcagaag
     6121 acaatacgct ttaagacatc aaaaaaatag ccctgactgg tatatcacca atcagcgcta
     6181 ctttgcatat tctcttttag tttcttatct caaatatcag ttaatggaca agcagtatct
     6241 ggagaggtat catggaccgt aaagtctgta cctactgcta aatctgccat agccagttga
     6301 ttgaccatcg tcatatgcgc taactctttg atgttatttt ctttactcag atgacctagg
     6361 tatatttttt tggtattgta ccccaggctt ctaatcatgg ctcccgctcc atcttcattt
     6421 gataaatgtc ctaaatcaga taaaatacgc tgcttcaagc tccaaggata agagcctgat
     6481 ctcaaaattt caatatcatg attggattca atcaagtagc catctgcatt ttcaataata
     6541 cctgtcatcc tatcactgac atatcctgta tctgtcaaca tcacaaaaga cttattatct
     6601 ttcataatcc gataaaattg aggatcaata gcgtcatggc tgactccaaa actttcgata
     6661 tcaacatctc caaaggtcaa aactttatcc ctttggaaaa tatgtttttg tgaggcatca
     6721 agcttaccta gcatattgca ctcatccatc agctgccatg ttttttcatt agcatagata
     6781 tccaaatggt atttgcgggc cataacgcct acacccttaa tatggtcaga gtgctcatgg
     6841 gtaatcaaaa tagcatccaa atcctcaggt ttgcggtcaa tttcagcaag aagactggta
     6901 attttcttac cagtaagccc tgcatcaatc aataagcgtt ttttaggtgt ttctaaataa
     6961 aaacaatttc ctgtggaccc agatgctaaa atactatatt taaaaccact ctcattcatt
     7021 gattagtcta catcttcctc ccattcttca tagatggctg catctttttc ataaggcaag
     7081 acaatagtaa aggtcgatcc tttaccatag tcactcttag cccagataaa gccatggtgc
     7141 tgcttgatga tttctttagc aatggcaagg cctagaccgg tccctccttg ggcacgactt
     7201 cttgccttgt ctacacgata gaaccgatca aaaataagag gcaaatctgt tttagggata
     7261 cctagtcctt ggtctgaaat ggaaataatt aattgggtat ctgttgtttt catacggact
     7321 gtgatttttc caccatctgg agaatactta atggcattgt tcaaaatatt ctcgataacc
     7381 tgtgtcattt tatcattatc aatttcaatc cagacagagg taatagggta atctcttaca
     7441 atttcataga cttttcctgt acctgtatgc tgatttttaa ccaaatcaaa tctgtttaaa
     7501 atagaagtta taaaagcagt aaaattagtc atctctactg ctaattgggt tacttgatta
     7561 tcaatccgag acaggtttaa aagatctgaa atcatacgca tcatccgatt agtttcatca
     7621 agagaaactt ttatgaaact tggagcaata tcttctttaa gtgcaccttc atcaagagcc
     7681 tccaagtagg atttaaccga agttaaaggg gtccttaatt catgacttac attagagaca
     7741 aaaagacggc gttcacgttc ttctttttct tgttctgtgg tgtcatggag caccacaacc
     7801 aagcccgaaa taaaaccact ctctctccta ttcaacgcaa agcgcaagcg taatgagaca
     7861 aactcaccca tatcatttcg gctattaaca gttaccactg gtgttttaga taccaaatca
     7921 cggtaggtat atgaagtatc accttctaac aaatctgtaa tgtttttctt tagtgcctct
     7981 tctttactta aatttaattg cttgcgagct gtctcgttaa tcatgatgat tttaccagac
     8041 cggtctgtag caagtacacc atctgacata taagccaaaa tacttgccaa gcgatttttt
     8101 tcttgggcaa gattttcatg cgtcaagcga aaaacatctg ataggtcatt taactggtct
     8161 gaaagctcca ttaactcaat gtctgctttt tcgtcgatta tatcagtata gcgaccatta
     8221 atcaagtctc ggattttatg gctcatcatc cgaataatac gtgcatttcg gtaatcacgc
     8281 accgcaagat ggataaagta aaaagcaaca aaaacaagta agataaggat cgctaattca
     8341 aatgtggata agtttccaat gatatctcta gtcataagat ttcatgtagt acccaacacc
     8401 acgtcttgtt aaaatatact caggacgact tggtgtgtct tcaatttttt cacggagacg
     8461 acgaacagtc acatcaaccg tgcgcacatc gccaaaataa tcatatcccc aaacaatttc
     8521 caataagtgt tctcgtgtca tcacctgacc catatggtta gctagatgat gcaatagttc
     8581 aaattcacga tgggtcaact ctacctcttg accatgtttt ttagcaacaa acgcatctgg
     8641 taaaatctgt aaattaccaa tggttagttc ctgtgtacct gaagaagcat tttcttctgc
     8701 aacagccgtt tcaatagttt cggtacgacg cagatgagcc ttgacacgtg ccagcaattc
     8761 ccgattagaa aagggcttgg tcacgtaatc atcagcccca atttcaagtc caataacctt
     8821 gtcaaactca ctatctttag ccgacaacat aataatcggg acatgactgg ttttacgaat
     8881 ttccttggct acttcaagac cgtccaactc agggagcatc aaatcaagaa taattaaatc
     8941 tggcttttct tcttcaaaaa ttgttaccgc ttcgcgtcca tcaaaagctg taacaatgtc
     9001 ataaccttct tttgtcaaat taaacttaat aatgtcagaa atcggttttt catcatccac
     9061 aataagtatt tttttcatgt ctatacctca gaaagttctt gtaattgata ttacaatttg
     9121 tgatttttgt ggttaataaa aaatcacaaa actatatttt tctaaattat ttctacctga
     9181 ttataccaaa aattcagcct aattgacaat gacgactacc gttacaaata gagtactttc
     9241 taaaaccaac cctttatagg atgaatttga cgtttgaatt gcttagcaga ttttttgact
     9301 ctggcaaaac tactcttgtt gtggtaagca aaaagtcctt tggcattaaa ataagagaac
     9361 ataaagtcat ctttttgagc tctaaaccac tgacgaaata ctctttttgg cgatcgaaat
     9421 cctttaaggg tgtcccttct aaactgagga tcgtgacggc taatcggatc aacatctaaa
     9481 tcccaatgtc taggaccatc tgtgccatag ttaaaagatg caccattgac aattcctttt
     9541 tcggtcacta cttctgcatc aacttcatta agaaaaaagc ctgtgctatc tagaataaat
     9601 tcggtatgaa accaaagaag tacttttata ttataggaaa cttcttgact tggaaaaatg
     9661 actttattat cttcaaatct tagtttatta tgtaacctgg cagaatccat caaggtataa
     9721 tctggtgctt gccgccacaa acaaggccct tttttagacc tcaaaaaaag ggccagagct
     9781 tgggcatctg tcatctcaat cattttataa tggtttctga tagattgagc ttgttgacta
     9841 gagatgacat aacgaaattg atgcaaacgc ttttcaagct cacctgcttg gcttagtgtt
     9901 ttgccaggaa aagcttgatc aactaagcta gccaactctt tccaaaacat atgatctggc
     9961 aatagagatg gatggaatcg ggtgagtagc gtttgagttt catgcaactg accacttagc
    10021 tcaaatggca tctctaccaa atcaagaaga aacgttagta actgcttagc tgattggttt
    10081 tctttttcaa taggccatat tacccagaaa gttggagaac caagtcgatt atcaatgata
    10141 ggctctttag ctatagcacg aaatgcttgg tttgataaac ctgcgttttg atagcatttc
    10201 aaggcctgat agcgatctaa gatagcttgt ttcatggcct tctcctcatc ttttttgacc
    10261 atattataac acatctaaag ctgaatcgtc acttgtatct cactataagc aaaacgaaac
    10321 ctgaagtggt taactaagcc actgtttggg tattttattt aaaagaactt ctcgattaat
    10381 tttaccatta tcttttaaag gcaaacaatc aatcacaaga tagtacttgg gaagttgtcc
    10441 agagtttaaa taaagagcta aaaagtcttt aataggtgct aggcaatcct tcttagtcaa
    10501 aactaatact aatattaggg tttcgttttc tccttttcct atctttaaag cgtgcgcttc
    10561 tttgacgttt ggcgcttggt gagcgagttc aactaggcta ggcaggtggc atttcactcc
    10621 tcgttgatta acccagtcat cttgtcgccc ttctaaaatt aaacccgctg ggctcatttt
    10681 tcctaagtct gagacactgt atggttgtga cattccttct acactataag gagtctctac
    10741 aaaaatttcc ttatctttga aactgataga cacatcagga aagggctgtc ctaccaaacc
    10801 attaattttg acagcagctc tgccattaca ccacgttata aaagataatt cactagcccc
    10861 gtagaaaatg acaatttcaa gctgaggaaa ttttttgtaa taatgtctta gtaattcttg
    10921 tgatatcatt tgcgaagagg tcaataagtg agtcgcagtc atgttgttct tagtcaaata
    10981 aggtagcaag cgatttaggt aggtcggtaa aaggtaaagg tgactcacct tttttgcttg
    11041 ccataagctt aaccacgttt tcaaagacag cttttggctt aaaaccagac atcccccggc
    11101 ccatagttga gctaatgcta agttaagatt gccagtaaaa ctaaaactac catgtaagaa
    11161 tagacaacta ttaccagtca taccaaacac cttattttga taatcaaaaa gtctcgtcca
    11221 agttgataga caacgccaaa ataattttgc tttgccagtt gttcccgaac tcaaaacagc
    11281 aaaatcagct tttttaagaa gctcactatc gacgtgtttt atttgctgct gaaatgtacc
    11341 tgagatatta gggtgtaaaa tgataggaca actgccttct tttaatcctc gtaaaaaaga
    11401 gagtaactga ttaagatagc gactatgact gatgatatat ggtacactgt cttttattgt
    11461 ctgatccttt attaaaaccg cttgccatag ctcttgataa gtcaagctta tctgatcggc
    11521 tacgatagct tttttatttg ggcattgctt tgcccaatat tctagctttg tcagcatgac
    11581 taaactcctt atgatatttt agtaaaacag caaacccttg gccaccagct gctgctatag
    11641 cagctattcc atagcgtcca tttttaatct ctaaagccct cattaagtgc aatattataa
    11701 tggctgctga ggcaccatag ggatgaccat aagctaatgc accaccaaat atattatagc
    11761 ggtccaataa atctggataa tgggtttcaa atagaccatc aataacggca aaggcttcgt
    11821 tccattcaat cgctgtcata tctgccatcc ctagaccatg tttttccaaa aggacttgac
    11881 tagccttaat caccattaaa gggctacgct gtgggtctcc tgctacttct accacatcaa
    11941 tcagtttaaa agctgaaggc tgactactta gctgtaaaaa ggcagcagca tcatgggtta
    12001 ggcaggcatt ggctgcacta atcacgtgat cttctcctaa gatagggggt acctttgaca
    12061 acaccttact tgacaagcgc ggtcgaatac cttgatcact ggctcctggc aggtctagaa
    12121 taagatcagc taacagggct tgctcctggc aataactagc cttttgatga cttctcaatg
    12181 tccagtgatt caaatactcc tttgtaaagc catgctctct tgccactcgt tctgcccctt
    12241 caatcatcgc aaaagggctg atactatctg gagaaaactg agctaccttg taggctcctt
    12301 ggcgccaatc agctgaagca tataccgatt caggttgtaa ggagctgctt tcaatccctc
    12361 ccactaaaag gttgcttgcc atacctgcct tgattttagc ataacccacg gaaagagctg
    12421 caccagctga tgcacactgc atatcaaccg tcaaagcaga gacagattct cctaaatgag
    12481 aatacagggt catcaggcgc ccaatattac cacccgttcc gacggcattg ccacaaatca
    12541 cttggtcaat gggaactgga tacttatttt gtaaagcatt gatgagcttt gctccaagga
    12601 tttctggttg ttctttagca aattgtttcc ctactaaacc gataggggtt ctaaggcctg
    12661 cagcaatata aacatccgtc attatattga cctcctttat tttcgatgtc cttaaatggt
    12721 tattatataa ttatttctga tggttttctt ttttgaacta ataactgcta aaaatacacc
    12781 taaattaatt tagagaatct ctttagtctc gctagtgtgc catcaataaa atctataaaa
    12841 ttctctaacg tcattgaaat gtcaagtccc acaactattg tcgtagaagt cacttacttt
    12901 gttgctttaa cattcaaact gtaacctttg tttttgatac agggtgactt tattatactg
    12961 gaaaaccatt cctaagaaaa gaaaatctta tatagaataa agaattagac accataaaaa
    13021 agctaagctg tgacacttaa cttttttatg gtattgacaa accttgtctt tactatttat
    13081 tgatagttaa tgcctctaac taaaataaaa gccatgtctg tatcagcctg caaggcatct
    13141 gctaagctgg tatcgttact agatttgaca ggtctcataa aggcaaaaga tctcaaagtt
    13201 ccataaacgt tatctagcaa ttgcatgcaa ccacttgaaa tatatctgct tatgttattt
    13261 taccgccctt aatttagacc aatctgctga tgatggttcc aaacaatgcc tctaggaacc
    13321 tgcactttca taagcatcta ggccccattt ccaaagctta agcgacaagg ccaaaaccag
    13381 taacgacacc actaccaagc ctccaatatt aaagagcagg tgctgacctg ttaaaaaata
    13441 agaagcaggg taataagctg taaaagcaaa gggaatgaca aaactgatta accaccgcaa
    13501 aaaggaatgg taaatggaca tagggtactt ggaaaagtca ttaaacatgt aaaaaatata
    13561 aataactgcc ccagactgct tggtccaaaa agaaatgctg gcggtcgcaa tttttaaaga
    13621 ggtgtaaatc aaggtcgcaa aaggaataac caaaataaac aaaagcacct taggaagagt
    13681 ccatacgata ctaccagccg tggtcactaa taagataacc ccaaccaata attcacccag
    13741 ggcatccact tgaaatgtct caaccaaaac atgaaacaaa ggactgattg gtctggtgag
    13801 gtacttgtca aattctcctt tgcggacaag ccgttgccct aaagcccaaa gattgtcaaa
    13861 aaagaggtga tcaattccct ttggaatcaa agaaaagcca taaataaagg caatttgttc
    13921 aaacgtccag ccttctaaag aagggatatg ttggaaaagt accgataaaa agagcagatt
    13981 aagtccctga gtgagaaaaa cacctagcac tcccaccaca aaatcaacct tgtattccat
    14041 aatttgtttg aggtattgtt tgataaaaat agcgtgcata cgtctcaatt ttgccatctc
    14101 accctccttg aatcgttaaa tggtactgaa cctttttcca gatgacttgc gataagacta
    14161 ccatcaccag taaccaaaag atttgcaaac ttagggccac cattatctga ctaaggctgt
    14221 atttcccaat gacaatcata actggcgtgt aaaccaaaga ggaaaacggt agaaaactaa
    14281 gcactatata aaccacttta ggaaaaaaag ccaatggaat caaactgcct gacataaagg
    14341 caaccaaggc atttttcaat aaattagacc cccaaaggtt tttaaagaca aatgctgaaa
    14401 agccaaaaca aatgttgaaa taaaaattga ttaaaaaagc taataaaagg ctaactaaat
    14461 acagacaact gctggctagg acttgcaagg tagacagccc cgccatgact ttaataccag
    14521 ataagaccat taaaaaagga aagccgacac tcattagcac aatccagcga aaaccaattt
    14581 ccataaagag gtaagaggct gcaaaatgaa ctggtcgaag caggcgcatg atgatagaac
    14641 catctttcac ttcttcgcca atcataaagg agctgtctga tttggtcagt aaagtggtca
    14701 caaaactcat gataatgtaa aaggtcatgt cagacagagt aaaaccattg attaaggatt
    14761 gcttagacga atcaaaaaca gccttccaga ggtaataagc cacaaaagcc cccatgacat
    14821 ctccaatacg atacaaaaag aaattaacgc gataggttat taattcctga attcctgcac
    14881 tgagaaaggg tctataacgt ttccaaaatg accacatctt aaagctcctt tcggtagaaa
    14941 cgacgaacaa tatcctcaat atctacatca gtcatcttca aatccctgac cgcaaaatca
    15001 gccatggttt tttgaataat gtccgctgtt tgatagcgcg aactgtcata ttgaatgtct
    15061 aaactaagct catgccgttc cactgtgatg tcaggtaagc ccataaactg tgagaccacc
    15121 tgctcctgcc ctggcttcaa ctcaaaagac agactcttca tttttccaaa gctttgcttt
    15181 aattgggtca ccgttccatc aaagatttct tgccctttat caatcatgat aatccgatca
    15241 cagagctgtt caatatcact caaatcatgg gtcgtcagta aaatagttgt cttttcttct
    15301 tgattaattt gagtaatggc acgccgaatg ttgtccttca cagaaacatc aagtccaatc
    15361 gttggttcat ctaaaaacaa tactttagga ttatgcaaca aggaagcagc aatatcagct
    15421 cgcatccgct gaccgagtga taaggtccgg acaggatcct taataaactc attcaggtcc
    15481 aaaacctcat ttaaaaaatc catgcgcttg cgaaaggctt tttcaggcac atcgtaaatt
    15541 tcttttaaaa cgacataggt ctcttgcaag gccaaatccc accatagttg tgttcgttga
    15601 ccaaaaacag ctccaatgtc tctcacataa tcttgacgat tatcttgtgg aatctttccg
    15661 ttaatgcgac agtaacctga tgtaggcttt aaaatacctg ttaacatctt aatcgttgtt
    15721 gattttcctg caccattggc tccaataaaa cctaaaattt gccctttggg tacctcaaaa
    15781 gataagtcct taaccgcctc aaagatttct ctttggggat gaacaaagga cttcaaggct
    15841 ccctttaatc ctggctcttt aatggttttg gaaaaatttt tttgaaggtg ggacacttct
    15901 atcatcacca tttaccattc ctcgtttctg ttatcgtctt atcaccgtta ctaaacaatt
    15961 tattataaca ccatgaaaaa gactgtgcaa ccgtttgctt aattttttaa gaaagaaaaa
    16021 gaagcccata aggacctctt tttgccattg attaagcttg tgcatctgga catgacttgc
    16081 gagcaatgtc tgccaagatg ttttccacaa attcttccac agattcagtg tgagtcgcct
    16141 tactaccata gcggcgaaca ttgactgatt tgtcttccat ttctttgtcc ccaacaatta
    16201 attggtaagg aattttgctg gtttgagacg cacgaatctt gtactgcatt ttttcattgc
    16261 ggtcatcgac atctgcacga acaccacggt cacgaagggt ttttgctact tcccaagcat
    16321 agtcaatgtg ggcttcattt gaaattggaa tcacggttac ttggtgaggt gccaaccaag
    16381 ttgggaaagc acccttgtaa gtttcaataa ggatagcagt gaaacgttcc atagttgaga
    16441 taacaccacg gtgaatcatg actgggcggt gttcttcgcc atctgcacca atgtatttaa
    16501 ggtcaaagcg ttctggcaac aagaagtcca actgaatggt tgatagggtt tcttcgttac
    16561 caagagctgt tttcacctga atatcaagct taggcccata aaaggctgct tcaccttctg
    16621 cttcaaagta atctacaccc atctcatcaa gtgcagcttt aagcatactt tgagcatttt
    16681 cccacatctc atcattgtca taatatttac gagtatcgtt agggtctcgg tatgacaaac
    16741 gaaaacgata atcagtcaag ttgaagtcag cataaacatc aatgattaat tgaagggcac
    16801 gttgaaattc ttcctggatt tgctctggtg ttacgaaaat gtggccatca ttcaaggtca
    16861 tttcacgtac ccgttgaagt cctgataggg cacctgattt ttcgtaacgg tgcatcatgc
    16921 caagctcggc aatacgaata ggcaattcac gataagaacg cacatgattt ttatacactt
    16981 gaatgtggtg tgggcagttc attggacgaa gaacaaactc ctcaccatct cccatatcca
    17041 ttacagggaa catatcttct tgataatgat cccaatgacc tgatgtcttg tacaattcta
    17101 cagaagcaag tggtggtgtg taaacgtgtt ggtagcctga tgctaattct ttatctgtga
    17161 tgtagcgctc aagtgtacga cgaatagtcg ctccatctgg taaccaaaac ggcaagcctt
    17221 gaccaacttc ttggctaatc atgaacaagt caagctcttt accaagctta cggtgatcac
    17281 gttctttggc ttcttctaga cgagtcaagt aagctttgag gtctttttta tcaaaccaag
    17341 ctgttccata gatacgttgc atcatgttat tgtcgctgtt accacgccag taagcacccg
    17401 ccacatttag caagtggaag acttggatac ggcctgttga tggcacatga gggccacggc
    17461 aaagatccac aaattcacct tgacggtaaa ccgtcaagcc agcaccagca tgctcattaa
    17521 tcaattctac cttgtaagga tcatctttga acaattccaa agcttcttct ttagtcactt
    17581 cttcacggat acatgggtaa ttttctgtca cgattttttg catttcagct tcgatacgtg
    17641 gcaaatcttc gtttgaaatt tggccttcag cattatctgt atcatagtaa aagccttctg
    17701 caatggcagg gcccacaccc aaatgaagat ttgggaacaa gcgttttgca gcttgggcaa
    17761 ataaatgggc tgcagagtgg cgaagcacct cataagcatc tttgtggtca ggtgtcacga
    17821 tttcaatact gccatcttct tcaatggcac gagtggtgtc aatgagttga tcattgaact
    17881 taccagctaa agctttttta gccaatgatt tgctgatgga ctcagcaatg tcaaacgttg
    17941 ttacaccaga ttcaaattca cgcacggcgc catctggaaa agtaatctta atcatttgct
    18001 ttctccttta agtcagttaa gatttccact aaaacatgga tagtaagaca aattagggac
    18061 aagaggttag tgaatagctc aataccacca cgtagaagaa caatacctta ttcacgaatc
    18121 taccctccct agtccttaaa aacaaggcta atcccctcca aaacaaaaaa actgaccgca
    18181 tcccaaagga cgcaatcagc gtggttccac cttaatttat gcagggcaaa aaacatgcct
    18241 tgcacctcta gtggcgataa gggggccacc cttttatgtt tgcataaaaa ccaggaagga
    18301 gtatcagatt agctatcctt gtgctctttc caccaaccag cactcgctta aaagattctg
    18361 tccaatctaa cttgtcttca agataattat agcaagtttt caaaaattta caagtcttaa
    18421 tccagaaaat ctttgatagc atggaccagc ttgataggag ccttgactac ttttactgag
    18481 gtgatagctg tgaccttagc agcacgttta ggcaagtgaa tagcaccttg cactaatttt
    18541 agagaactac ttttaccaga atgattaaga taaagactca gcttttgagc ttctttgcta
    18601 tttctagcaa ttaacgtatc taaatagaac gtgtaaatag attttccaaa gtgctgtgct
    18661 gagatttcat aacgtttttt tgctaataac cgtttatcca taactggtgt ttttagtatg
    18721 gcatcaataa tagcatcagt taaatctgtt tcagcgtaat aaagagtgcc aaacatttta
    18781 tcagtcacca catcatctaa ataaggattg ccatgagcaa taataggagt gccactagcc
    18841 aaactttcaa tataggtcaa gccctgagtc tcactggtcg atgctgagat aaagaaatca
    18901 caagccttat agtacagagc aaccttatca tgaggcacca tgcctgtaaa ggtcacgtgt
    18961 ttgtcaacct ctaactgcat cgccaagtgt ttcaaatctt gcaaataggg gccatctcct
    19021 acaataataa gctttatctt ggcattttca gccaaaatag ctggcatctg attgatgata
    19081 gcttgaatat ttttttcata agaaatccgt gacaaactca ataacatggt ttcatcacca
    19141 gcaatgccca attctgcttt taagttggtt acttcttctg ctgtgatgtc atcacgaata
    19201 tatttttcca aaggaatgcc tgttgggata acccgcttag ggatagtaac ttcgtaacct
    19261 tctagaagat tgaggacaat cctacttggg cagataaccc catccaaatc cttcaaatag
    19321 cccctaagaa gaggtttgac catacttggt cgaatgattt ttccgttggc aatataactc
    19381 acgtagtcct catattgggt atggtaagta tggacaacag gaattcgcaa agctttgcct
    19441 atcattttcc ctaacaagcc taagctaaac tcagtttgcg tatgaataat atcaagatta
    19501 tagtgttttg caattttgta tgacgaaatg aggccacgat aaaccacacg tctatccgta
    19561 aatgacacaa aaggaacact tggcagtcga ataatggtcg ggtcttcaaa gcgtttgaca
    19621 tctctatcag tagtggtgaa aatataaact tcgtgacctt ctttttctag ctcttctttt
    19681 aacgtacgaa tactagtagc gactcctgaa acttgtggaa aataggtatc tgtgaataga
    19741 cctacacgca tcttataact ccattacttt tttatagaca tctactaagg catggccaac
    19801 cgtttctagg cgacgactct gggcaacctt gtaaccagct tcaactttgt tgctggcacc
    19861 agaaaagact tttttcagag cttctataaa acctggtata tcggttgcta attccgcact
    19921 actttgatca acccatccgt agtaaactgg tatatcacgt aaaacaaggt gctgacgact
    19981 ggccaaggct tctaagacaa caatgccttc tgtttcttca cgacttggaa agaaaaaggc
    20041 atctgcacca gtcatggcac cttcataaac atccccttta atgtatcctg ggaaaataag
    20101 atttttcggg tggttaccat tgaccatttg gcgaacttga gcaggaatga cccatttgtt
    20161 ggtctcgcca aaccagataa aacgaacatc tggcatagct tgggcaactt tgacaaagtc
    20221 atcaattcct ttcctcagaa aaaataatcc tgctcccata accacttttt caccctcttt
    20281 aatgtcaaag tagcgacgaa aagctgcttc ctttttagaa tctgctccgt actgctccaa
    20341 atcaatccca tttgacactg caaaaatagg tgacttcact ccataactct caatcaaaga
    20401 cttagaatat agggtagggg taatgatagc atctgcctta ttgtaaaagt gacaaaggta
    20461 ttttttaaac caaggagata atagatttga aaaaataaaa gaatttctaa aatcttcttc
    20521 tgtagaatgc ccatgcatga taaccttctt accagctttt tgtgctttta tcatcagcag
    20581 ccaacttttt aaaccatagg tattgagatg aaccaaatca taagtttctc ttggattagt
    20641 cgtaaaatgt tgaccaacaa gtgacaaggc tttagcctga tgcttaatcg ctcgaccaat
    20701 tcctgatttt cttagataat tttctgcttc taaatacaat aagactttca taagttctat
    20761 tatagctcat tttatagtat ttttcactaa gacatgcaaa aatcagccct aaggctgatt
    20821 ttagtattga agaaacagtc tattgtgata ctttttgtta gatggtgctc ataagtcaca
    20881 gttacttagt tgtcccacgt tttttaatac catgattcaa aagaatttct ttttcttcca
    20941 actcttcttt gttcatgatt ttagtcaaca tccgcatgct aacagcacct aagtcataaa
    21001 caggttgact gatagaactc aaattgggac gagtgtattg aaccactggt gaatcgttgc
    21061 tggtgatgat ttcaaagtcc tctggtacgc gtttacctgc ttcaaacaag ccgtttaaaa
    21121 ggcctgctgc caattcatct tcagccacat aagccgctgt tgctcctgag ttaatgacgc
    21181 gctgcgccaa ttcaaatcct tctttataag aatagttcgc ttcaaaaaca agaccttctt
    21241 tgaagtcaag cttattgtgc ttcaaccctt ctttgtaacc tgctaagcgc actttaccat
    21301 tgatatcatc aatgagtggt cctgacacaa aagcgataca cttatgattt tcagctaaaa
    21361 tatcaacaac gtttgacacc gcagctctat agtcaatgtt gacgcttggt aattgatgat
    21421 caagatctac agttcctgcc aaaacaactg gagtacgaga gcgtgaaaac tcggcccgga
    21481 ttttttctgt caagtgatgc cccataaaaa taataccatc cacttgcttg gcaaaaaggg
    21541 tattgacaac attaacctcc ttatcatcat cttcatcact ggaggcaagc acaatattat
    21601 atttgtacat agcggcgata tcgtcaatac ccttagctaa gatagaaaaa taactatttg
    21661 caatatttgg aatcacaacg ccaacggttg ttgtcttttt gctggcgaga ccacgcgcga
    21721 cagcattggg acgatagtca aggcgatcaa taacttctaa aactttctta cgtgtatttt
    21781 ccttaacatt tttattgcca ttaacaacac gactaacggt tgccattgaa acaccagctt
    21841 cacgggcaac atcataaatt gtaatggtat catctgtatt cattttttat cttcctttct
    21901 atttgaaaat gacgctttca aaaagtattt tagcatgatt tgtaaccact ttcaagtgtt
    21961 ataggttaat ttttgaaaaa gttttcaaaa attaactcga agcttgattt tttaagcttt
    22021 ttttgaaaaa atagtaagta gaaagaaggt tagcctatga caaaattaga ccaaatacgt
    22081 ttgtatcttg accaaaaggg agctgaattg gcgattttct cagatcctgt tacgattaac
    22141 tatttaactg gattcttctg tgacccacat gaaagacaac tgttcttgtt tgtctaccat
    22201 gatttagctc ctgtgctttt tgtacctgcc cttgaggtgg ccagagctag ccaggccata
    22261 tcatttcctg tgtttggtta tgttgattct gaaaatcctt gggaaaagat caaggccgtt
    22321 ttaccaaata cagcagccaa aaccatttat gttgaatttg accaccttaa cgtcaataag
    22381 ttccatggct tgcagactat tttttcagga caatttaaca atctaacccc ttatgtccaa
    22441 ggcatgcgcc tcgtcaaatc agctgatgaa atcaataaaa tgatgattgc cgggcagttc
    22501 gctgataagg ctgttcaagt tggttttgac aatatttcct tagatgctac cgaaacagac
    22561 gtcatcgctc aaattgagtt tgaaatgaaa aaacaaggca ttcataaaat gagttttgac
    22621 actatggtct taactggaaa taatgcagcc aatccccatg gcattcctgg aacaaataac
    22681 attgaaaaca atgcccttct tctttttgac ttaggtgtcg agaccttagg ctacaccagt
    22741 gatatgacca gaactgtggc tgttggtcag ccagaccaat ttaaaatcga tatctacaat
    22801 ctctgcctag aggcacaact agcggctatt gactttatca aaccaggtgt aacagctgct
    22861 caagtagatg ctgctgctcg tcaagtgatt gaaaaggctg gatatggtga gtacttcaac
    22921 caccgcttgg gacatggtat tggcatggat gtccacgaat ttccctccat catggctggc
    22981 aatgacttgg tccttgaaga aggcatgtgc ttctctgttg aacctggtat ttacattcca
    23041 ggaaaggtcg gtgtgcgcat cgaagactgt ggccatgtca ctaaaaatgg ctttgaagtc
    23101 tttacccata ctccaaaaga gttgctttat tttgaaggct aagcccctat catctggtta
    23161 aacaaaccct cgcgaaagga aggtattcgt atggatcaaa ccattcatca ccaaatacag
    23221 caagcactac actttagaac agccgttcgt gtttataagg aagaaaagat ttctgatgag
    23281 gatttagccc ttatccttga tgctgcttgg ttaagtcctt cttctgttgg cttagaaggc
    23341 tggcgctttg tcgttttaga caacaagcct attaaagaag aaatcaagcc ctttgcctgg
    23401 ggggcccagt atcaactgga gacagctagt cactttattc ttttaatagc cgaaaaacat
    23461 gcaagatacg atagccctgc tatcaaaaat agccttttac ggcgtggtat caaagaaggt
    23521 gatggtctca acagccgcct aaaactctat gaatctttcc aaaaagagga catggatatg
    23581 gcagataatc ctcgggcact ctttgattgg acagctaaac aaacctatat cgctcttggt
    23641 aatatgatga tgacagctgc tcttttgggc attgatactt gccctattga aggctttcat
    23701 tatgataagg tcaatcatat cctagctaag cataatgtga ttgatttaga aaaagaaggc
    23761 attgctagca tgttatccct aggctatcgc ctgcgagatc ccaaacatgc tcaagttcgt
    23821 aagcctaaag aagaagtgat ttcagtcgtt aaataaggag attaaaatga aagcattaca
    23881 tacctgtatc cgcgtcaaag accttgacca gtcggttgct ttttacacaa gcgccttccc
    23941 ttttaaggaa aactatcgta aagattttcc agatagtcaa tttactcttg tttatctggc
    24001 tttagaagga gaaagttatg aattagaatt aacctacaat tatggtcatg gcgactacga
    24061 cttaggaaat ggctatggtc atattgccct tggctctgaa cattttgaag cagaccataa
    24121 aaaacatcga caagcaggtt ttccagttac cgatatcaaa gaactggctg acaaatctgc
    24181 ccgctattat ttcattcagg atccagatgg ctacaaaatt gaagtcattg atttaaacaa
    24241 ctaatactat taacaacaga agctttaaag cttctgttgt tttatttttt aacctgacga
    24301 ttgggacaat ctcattatta acagagtttt gaaatatctc agcttttgtg ttacaattgc
    24361 cctaaaattc catttgaaag aaaagtctat gacactacta tctatcattg tcccctgctt
    24421 taatgaagaa gcaaacattc tcccttattt tgaagaaatg catcaactag aaacgtccat
    24481 gaccaaccaa cttgcttttg aatacatttt tattgatgat ggttctaagg ataacacctt
    24541 gggtattttg cgtgagctag ctgctcgctt tccaaatgtg cattatctgt ctttttcacg
    24601 ccattttggc aaggaagctg gactcttagc tggactaaaa gaagccaaag gcaattacat
    24661 tactgtgatg gatgtggatc tgcaagatcc acccgaactt ttgccagtta tgtacgctaa
    24721 gctaaaagag ggttatgaca tcgttggcac caggcgccaa aatagacaag gagaaccact
    24781 aattcgttca atgtgttcga atctatttta tggactgatc aaacacttat cagatactga
    24841 aatggtcaat ggagttcggg attatcggtt aatgaccaga caagtcgttg atagcattct
    24901 tgagttggga gaggttaatc gtttttcaaa agggatcttt tcgtgggttg gttatcgtat
    24961 cacctatctt agttttgaaa atcaaaaacg caaatatgga aaaagtcgtt ggcatttttg
    25021 ggagttgctc cgatattctt tagatggctt tatcaacttt tcagaaatgc cgttaaccat
    25081 tgctacctgg acagggactt tcagcttttt gatttctatt ttcgccatct tatttatcat
    25141 tatcagaaag atactctttg gtgatcctgt ctctggttgg gccagcactg tctctattat
    25201 tttattcatg ggaggcattc agctcttttg catgggtatt attggaaaat atatttcaaa
    25261 aatattcctt gaaactaaaa aaaggcctct ctatattatc aaagaaaaac attaaattaa
    25321 aacatttttc tcatcaatcg tttcctactc cttctgtgcg cgaagctttc tttttaagcc
    25381 cgttatacca aatgataatg acctttccaa aatagtatca ctttccttaa cactttaagt
    25441 agaaaaggaa ctattaggtt gagccttttt gcaaatccta gcgactaatg ttataatcgt
    25501 catactattg aaaaaggagt tctttatgga gtggattaaa ttaatcggta ttgtgattat
    25561 cgtattaggg tttattttaa aatttgatgc tatcgctacc gtagttgttg caggacttgt
    25621 taccgcgctg gtttcaggaa tttcgtttat tgatttttta gatattttag gaaaagaatt
    25681 taccaatcaa cgtttgctaa ccattttctt catcacttta ccgcttatcg gcttgtcaga
    25741 aacttatggg ctgaaacacc gagctactca gttgattcaa cgtgttcaag cccttaccgt
    25801 cggccgttta ttgactctct accttattat tcgagaatta gcaggtctct tttcgattcg
    25861 actaggggga catccacagt tcgttcgccc cttgattcaa cctatgggag aagctgctgc
    25921 taaagccaac attggagaag agttgaccga tgcagaaaaa gatgacatca aagctatggc
    25981 agctgccaat gaaaactttg gcaatttctt tgcccaaaat acctttgttg gcgcaggagg
    26041 tgtcctactt atcgctggaa ctttagaaca gcttggctat gatggtaacc aagctaaaat
    26101 tgcattttct tctattttaa ttgctattat ctctattatt atagttgcta tttacaatta
    26161 cctctttgag aaaaaaatgg aacgtcaaca tcagaaagga gataactaat ggctaacatt
    26221 gcaaaccttg ttttagaaac gatatatatc cttatcggct tgcaactgtt tcacacagcc
    26281 tactgcacct ttaaagataa aaccaaccct gtctattttg ggacagctct gttctgggga
    26341 ttgctgggtg ttaccttcgt tggcggtgcc tttttgccta ataaagttat cggttttatc
    26401 gtgattgtcc tagctctttt aaccttgttt aagcaagttc gtattggaac attaccagcc
    26461 tttaatgaac aaaaagctga agaaagtgct caccgcattg gtaactggat tttcttacca
    26521 gttatgttaa tggctatgat ttctctactt cttgccttga tccttcctga tttcagtaaa
    26581 agtgccattg ggattgctgg tatcttagcc actattgcta ttttaataat cactaagcaa
    26641 aaaccatctg ctcttttagc tgaaaataat cggatgaacc aacaagtgtc cacaagcggt
    26701 attttgccac agctcttagg agctttagga gctatttttg cagctgctgg tgttggtgat
    26761 gtgattgctt ctctcattcg ggagattgta cctgctgata gccgcttctt tggtgttctc
    26821 gcttatgtgc ttggcatggt tatttttact atgattatgg gaaacgcttt tgctgccttt
    26881 accgttatta caaccggtat cggtgttcct tttgtttttg ctttaggagc tgaccctatt
    26941 attgccggtg ctttagctat gactgctggt ttttgtggta ccttattaac accaatggca
    27001 gcaaacttta atgccttgcc tgttgctttg atggagatca aagatcgaaa tgcagttatc
    27061 aaaaagcaag ctcctatcgc ccttgtttta attattagtc acattgcctt aatgtattta
    27121 cttgcttatt attaactttt ttgctaagtt agtagcgtca gttattcatt gaaaggacat
    27181 tattatgaaa attcttgtaa caggctttga tccctttggc ggcgaagcta ttaatcctgc
    27241 ccttgaagct atcaagaaat tgccagcaac cattcatgga gcagaaatca aatgtattga
    27301 agttccaacg gtttttcaaa aatctgccga tgtgctccag cagcatatcg aaagctttca
    27361 acctgatgca gtcctttgta ttgggcaagc tggtggccgg actggactaa cgccagaacg
    27421 cgttgccatt aatcaagacg atgctcgcat tcctgataac gaagggaatc agcctattga
    27481 tacacctatt cgtgcagatg gtaaagcagc ttatttttca accttgccaa tcaaagcgat
    27541 ggttgctgcc attcatcagg ctgggcttcc tgcttctgtt tctaatacag ctggtacctt
    27601 tgtttgcaat catttgatgt atcaagccct ttacttagtg gataaatatt gtccaaatgc
    27661 caaagctggg tttatgcata ttccctttat gatggaacag gttgttgata aacctaatac
    27721 agctgccatg aacctcgatg atattacaag aggaattgag gctgctattt ttgccattgt
    27781 cgatttcaaa gatcgttccg atttaaaacg tgtaggaggc gctactcact gaccgtgacg
    27841 ctactaaacc tattttaaaa aaacagagat atgaactaac tctgtttttt ttgtgctaaa
    27901 aatgaaagac ctagggaaac ttttcatcgg tctttctcaa ttgtcatctt aatctaatac
    27961 tacttctaac atcagcgggt atagtttgcc agtaattaag aaacgttgtt gatctaaatg
    28021 agcaatccca ttcaaaacat taaggtcagg gtaatgggac ttatcaagat ttaaggcttt
    28081 taacaaagga ctaatatcat aggtggctac cacctttcca gaatcaggtt ggagtttgac
    28141 aatagtattg gtttgccaaa tattggcata gagataacca tctacatact ctaattcgtt
    28201 aagcattgag atggggacac tttctatagc aactagtacg gtatccaata aagcaaaatc
    28261 tttggggtct cgcttttgca aaaaagcatt tccactagtc atccaaagac actggttttc
    28321 cttatcatag gctaatcccc agccatctcc ttcaaatgga taggccccta aacaattgca
    28381 ggtagcctta tcaaacttgt aggctacacc ctctttatag gttagtaacc aaaaataatc
    28441 ctcaacaact gttagccctt ctgcaaaaac tgtatctgga aaagctattt tttctgaaaa
    28501 gatctcttga gtaaggtcat acacccctac ttttgagaaa ccataacgac cggcactaag
    28561 taatatatga ttgttgttga gttgttctaa tccctgcgtg tataagttgc tatcataaga
    28621 ataagttcgt aacaaacgaa cttgtccttt agttatcata atactcattc accttacctt
    28681 gcattgtagt gtttcatttg ttttttaata tctctttcct gatcacggcg tttaatggtt
    28741 tcgcgtttgt catactcgtg tttcccttta gctagaccaa tcagaacttt agcaaagcca
    28801 tccttgaggt aaactttgag aggaaccaaa gtcataccag atcctttaag ttcatttgct
    28861 agatgcgtaa tctcacgttt tttgagcaac agtttgcggg tccgttcagg atcagcgttc
    28921 caaatattac cttgttcaaa aggtgcaata tgcacgttga ccaaccacgc ttccccattc
    28981 ttaatttggg caaagccatc tttcagttga atacgagctg cacgaacact tttaatctca
    29041 gttcccgtta agacaatccc tgcttcaacg gtttcaacaa tgtgataatc atgtctagcc
    29101 tttttatttt gcgccagtat atgaccttca ccttttgcca tagttaactc cttttacgac
    29161 tcttcttttt tgcttgttct ttgtaaaatg gttttttaga ccctttttta gttttaccct
    29221 tggtttttgc cttggctact tctttaggtt cttttttctt tgtcccttta gatgattttg
    29281 aggatttacg gcgatctcgt cgactagctt tatccgacat cttgattttt tcaaccacat
    29341 caaagtcgct aggtaaatac tcaaagtcaa tgtcaccagt ttccttgtca gccttaacca
    29401 atttcacacg aataggttga ccaactttaa agactttacc tgatttttca ccttgaaggc
    29461 ttagcgttct ttcattaaag tgatagtatt ccggtaagct agtgatatga accaaacctt
    29521 caatcgtatt tggcaattct acaaaaaaac caaatttaac gacactggcc acgataccgt
    29581 caaattcttc acccacatac tctgccatat actcagcttt tttcatggct tcaaccacgc
    29641 gttcagcatc aatagcacga cgctctaact gtgaagaaga tgttgctaat tctggaatga
    29701 tctgagtaaa atggtcgcgc ttttcttgac taggttgact gtactcacga accatgcggt
    29761 gaaccaacaa gtcagggtaa cgacgaatcg gactggtaaa gtgagtgtaa tattctgcgg
    29821 ccaaaccgta atgcccatga ttatgttcag aatatcttgc ctgttgcata gagcgtaaga
    29881 gcatcatgtt aagcacttct gctcctggtt gaccttcgac ttttgccata aaggcttgca
    29941 aggcttcctg agaaatcttg ttggctgttc cttgaatgtg aataccaaag gtgctagcgt
    30001 aatcaataaa ttgttgaagt ttttcggcct ttggttcttc gtgaatacgg tagataaacg
    30061 gtaacttagc ttttgcaaaa tgttctgcca cacactcatt ggctgctagc ataaaggact
    30121 caatcatccg ctctgcaatt cccctttgac gaagcaccac atcaaccggc attcctttgt
    30181 cattaacaat aactttagct tcttgcgtat caaaattcaa ggcgcctcgt tttgaccgca
    30241 tagcttccaa aatcctatgc aaagccacca tgagggtcac atcatcagca atgctagcga
    30301 attcttggag cgcttcttca tctcctgcaa tcatgtcatt gaccgtacta tacgtcattc
    30361 ggtaagttgt cttaatcact gattgacaga tttgataatt gaccacatgc ccttgagaat
    30421 taatttccat caaggctgac tgggtcaaac gatcaacgtt agggttcaaa gagcagatgc
    30481 catttgacaa acgctctggt aacatcggaa ccacacgatc ggtgacatag acagaagtgc
    30541 cacgggcgat agcttcctta tcaagggcag atccttctgt cacataatat gagacatccg
    30601 caatatgtac cccaagttca tagttaccgt tatccaatag cttaatatgg atagcatcat
    30661 ctaagtcttt ggcgtccgca ccatcaatag taatggtggt ttcttgacgc aaatccacac
    30721 ggcctattaa atctttagca gttggtgctt ctgaaatagc attggcctca gctaaaactt
    30781 ctgctggaaa ttctgacacg atatccatcg attcaaggac ttctaggaca tcaattccaa
    30841 cgtctccttg atggcctacg atatcacgca cactggctac aaaataatcg tgcccgcgtg
    30901 tcgggtattt atcaatatca accttgatga tttcagtgcc tttaagcaca acaggttctt
    30961 ttttgatata gattttttgt tgaatctttt ggtttttgga cctgatataa ccagcatatt
    31021 ttggtttgtc atcatctaaa ataaaggttc caacagctgt ttttaaagaa cggtcaacaa
    31081 tagcaacgac cttagcttca gccgctgtgc ctttgagacg gtctgctggc tttttaacca
    31141 ctacttcgac agtatcacca tcaatagcat acccaacatc attacgaccg ataaacatgt
    31201 catcttcatt ctcatccaca tgtaagaaac caaagcctgc tttattggca cgaaaaacac
    31261 cctgaacagt aggttctttt ttcttttcac gctcttttct aagagccaga ctgccatcat
    31321 ctgaaaaccg taacaggctc tgactttcca ttttggaaat ggttttaata aggctgggaa
    31381 aatgcttagc tccagccatc tccaaggccg cagccaagtc gttcacattg gacttgccat
    31441 gctccttgag gtaagtcata attctttctt tcatcatacc taatttctat ttctgttttg
    31501 tttgttggag gatatcaatc cgtttcgctg ataaaaaaat gagctagact aactctgtca
    31561 agctcactcc ttatttactt gataaaatgg caagcacaag tgcaatcgct agccaaaaaa
    31621 atactagaac tgctgtgaat cgttgcatga atgcttcaaa gccacgagct tttgtacgtt
    31681 caaacaaggc ttctgaaccg ctgctatcaa aaacattgct acttgggttt ttttgaggtt
    31741 gcataaaaat agcaatcaca agcaataccg acagtactag caatatcgta agtagtaagt
    31801 tgtacatgtt atttcctcca ctattgtcat gaccattcta acacaattct tatcaagttt
    31861 caacatgcag ggtcactttt ctctccttgg ggcagtattt gggaactttt attttttccg
    31921 gatgactgct tttattttta ctggttagat aattgttact gccacactca ctacattcta
    31981 aattaatttt tactctcaca caatatcctt aactttaata tattttcgaa atataatcaa
    32041 actccatatt aaattaacaa acacaatcaa actagtcaca taaaaaacag aacggtagcc
    32101 taacactaca gccacatttg atccgataaa agggccaatg acttgaccaa ggttactaaa
    32161 catttgatta taggcaaata ctctagaaat accttctttt ggtgttaact tagttagtaa
    32221 agagttaata cttggcatca aagcgcccac accaaaccca taagcaaagc gtagtactcc
    32281 taattgaaaa ctggtctgcg ctaaggcact gctaaaatac atgataaagc tataacacaa
    32341 agcggctaac aataaccgat gattgccaaa acgatctcct aattttccta agtaagaact
    32401 gctaaataag ctagaaaacc ccatggccga aactaccagg cctgaagtaa acattaagtt
    32461 gtgagtttga ccaagatgac gaatatacaa tgataaaatg ggggccactg attgagctga
    32521 aatttgaata atcatactag tcacaaacaa accaagcata atctggggac tttttacctg
    32581 ttttaaaatg acttttgtag gtatcatctc aaagcgccta atcggcttaa actcttcttt
    32641 aacataaaca gctgtcatta aagaacataa aaataatatc acaccaacta ataagaaaac
    32701 ttgtcgaatt cctaatagct ctgccaaaat cccccctagt aaaggaccaa ttaaagcgcc
    32761 tgctgtcact cctgttgcta atgtgcctaa agcataacct gactcctctt ttggggcttg
    32821 gctggcaatc aaggcagtgg catttggcac atagcccgca aagacacctg ttaataatct
    32881 caaaatgagt aaccaaaaca cattaggaat aatagctaaa ccacccatgg taaaggtcat
    32941 gacaaaactt gctcgcagca tcataggttt tcggccataa cgatcagcca acttccccca
    33001 aacaggagca aataaagctg aagcaagtgc cgtcacagca accgccagac cagcatagta
    33061 ttctactaat tctttcggag tacctaaatt ttccacatac agagccataa aaggcataac
    33121 aagtgaaaag cttgccccag taaagaagtt accaagccaa gccactttta gattttgccg
    33181 ccagttcact gtcgttaccg ctttatcact aatagatcat catcttcttt ccatcgggcc
    33241 tttgattatt ataacacatt tctgccaaaa acagtgtccc ataatagtgc taagtctcat
    33301 ctgatagcct tcatcgttta cgtaagctac ccagtaaacc tctgaccagt tctcgcccaa
    33361 ttgttctaac cgtcgtacta ataaacgcat ctgttgcttt ttcgacgact gtttttcgtg
    33421 gacgcccaac agaacggcga ctagcttttt tggtagctgc ttgcgccttg atggcttctt
    33481 tagcttcttt ttcgcgtttt gctgtcgcta tcgcttcttg ggccagtcgg ttgtcttcta
    33541 aaaccttagc agctaatttt tcataagcag attcacggtc aatggactga ctatatttac
    33601 tggcaaaagg tgaagactgc acaagctgct gactttctat ctctgacaaa acagcaaagc
    33661 tactcttagg cggcatgata taggcacgtt caacgatgga tggttgtccc ttatcattca
    33721 aaacagaaat caaggcctca ccgacttcta gttctgtaat cacccttgcc acatctaagt
    33781 caggattttg tcggaaagta tccgcagcga cacgaaccgc cttttgttct ttcggtgtat
    33841 aagcgcgcaa ggcatgctga atacgatttc caagctgagc caaaaccgtt tctggcaaat
    33901 caagcgggtt ttgaatcaca aagaaaatcc caactccctt tgaacgaata agtcgcacaa
    33961 tctgttcaac tttttccaaa aagaccttag gagcgtcctt gaacaatagg tgagcttcat
    34021 caaaaaagaa aaccatctta ggtttgtcca aatccccaac ttctggcaat aattcgtaca
    34081 actcagacaa gagccaaagt aagaaggttg tgtaaagggt aggagattga aaaagcttag
    34141 tcgctgacaa aatgttaatg gctccataac ccgaagcaac atctaattgc atcaaatcag
    34201 ccacatctaa agcaggctct ccaaaaaatt ggtgggcccc ttcttgttct aatgtcaaga
    34261 gacttctttg gatagccccg atagattgct tggcgatatt accgtaatgg ctggagtagt
    34321 ctgaggcatg gtcaccaact tcttttaaga tagcctgcaa gtctttcaaa tcaatcaaaa
    34381 gccaaccttt ctcgtcggcg attttaaaga cgatgtttaa gacacctgtc tgggtatcat
    34441 taagattcaa taaacgcgac aacatcatcg gtcccatttc agaaattgtt gtccgtaagg
    34501 gttgaccgtt ttgaccaaaa acatcccaca ttcttacagg aaaggcctga ggctggtaat
    34561 cacttacccc gattgtggct aaacgcgcag ccaatttatc agtcacctct cctgctttag
    34621 taagattaga caggtctcct ttgatatctg ccaaaaagac tggcacacca gctaaactta
    34681 attgctctgc tagcactttt aaggtcacag ttttacctgt tccagtcgct ccagcaatca
    34741 aaccgtgacg atttagcatc cctaattgta aggctatcgg ttgactgcct tttgcaattg
    34801 tcattgtagt cataggcttc ctcttttttg atgatcatac ttgtcgatat gtgtttaccc
    34861 ataagttaaa gtgtttagaa gggtctcact cttaatacta ttataacata taacccttga
    34921 tgcgctaatt taaaagggac cagctagttt tagacagaaa tagcttgatt ttgacagcta
    34981 tgatacgaat ctctcagctt agctacctcg gattggctaa tgacaacaac gcagattgta
    35041 cttgctttat taaggctgca atatccccac tattatcaat cactagactg gcatagggct
    35101 ttttttcctc tattggtaac tgactggcta ttcgctgcct tgcttttcct ttgtctaaac
    35161 gatttctagc cattaagcgc tgcaattgtg tctgagcatc tacataaacc agccaaatcg
    35221 catcaaacca gtcttggtag cccaattcca ttaaaatggg aatatccatg aaaaaaatgg
    35281 cttggctctg tgctaagtgg tcacgcttag ccgccaattc ttccttaatg atttgatttt
    35341 gaatggctga ggatgttgcc atattatctg ggtttgaaaa aagcatttct gacagctttg
    35401 tgcgatctaa ctcaccatct gcttttaaaa tctgattccc aaaagcttct ctaagagcct
    35461 cgtaaagacg accgcctttt tcttgcaagt catggaccac ttgatccgca tcaatcacct
    35521 gataaccagc ttttctaatg accttaacca cagtcgattt tcctgatgca atgcctcctg
    35581 tgatgccaat aatcatggtc tttttttctg gcattttgga caaatgtgcg tcccccttcc
    35641 tccaactttt aatttgacaa tagcctgtcc acaacgagga cacggtttcc cagtttgacc
    35701 ataaacctgc aaatagtcct gcatggtgcc atcagctccc aaagcattac ggtaagtacg
    35761 gacggtcgaa ccgccctttt caattcctaa agctaggatt cgtatggttt catcatgcag
    35821 ccgttttatc tccgccttat tcaacctaga agacgcagtt cctggatgaa ccttagcagc
    35881 ccaaagtacc tcgtccacat aaatattccc taaaccagca accaaggtct ggtctaacag
    35941 atgtggtttt atgggttttt gggaggatag cagagcagcc tcaaaggttt ttaacttaaa
    36001 ggtctctttc tttggttcag ggcctagttt tcgtttggca aaaaacgctg ataattgaga
    36061 ttttgctatc aaatcaaagg ttccaaattt acgcacatct tggtaaacca aggtcgaacc
    36121 atttttcaat tcaaaaaaga catgaaagtg tttgttatca gggacctcat ctggaaaaag
    36181 caaatatttg ccttccatcc gcaaatgtga taccaacact aattgcccca aatcaattaa
    36241 taaatatttt ccccgacggc caactgactg aatgatttga ccaggtaggg tcagggcaaa
    36301 cgtctctaag tcggttttaa ccatctttgg cactttaagc gtaacagcca caatttcttg
    36361 gccaagcact aaagtctcaa gccctcgtcg gaccgtttca acttctggta attctggcat
    36421 aatccgttat ttaaaggaca gctagaccct ttgctcctac aaagaataaa ctcagtataa
    36481 aaaagtcact caatagactc aggtctaggc tattgactgc gtttcaaaaa acacagctgt
    36541 ccttctcctt tcttatttct tattttacga acacatctga tagaaagcaa ctcataaatc
    36601 tgcctcagac cagtcctcat cccccttacc tcccagcact atttggcggt aatgttcctt
    36661 ataactataa tacaatgagt cctctccaag atacttaaaa atctgcaagg cctgccgcat
    36721 gtcctcttct ccagacattt cttcctgctt taatttataa tacccatgca catataagaa
    36781 aacagtcttt tcataaaaat tgagctcgtc tcgcagcaaa agatcaattt tgttaatcaa
    36841 atagcgacta tgttcaaagc aagaatggtc aatactgata atgagacaat taatggataa
    36901 ctgagtcact aacattttat tggttttatt ttgttccgaa taagcaaaag atgctaccat
    36961 ttccttagtc aataaaaata gggtattgta attcatgaag cgcgagcaat tccccagaag
    37021 aatgatttca taatatcccc actgttccac tttaaataaa taatctgtga ggcgagtcaa
    37081 ttcttcttga ctaggtgcaa ttgaggagtc aatagaaaaa agaatagctt taatcatcgt
    37141 tcgttcataa tctttatggg cataatcctt caacagtttg gtcaatttaa ccacgttttt
    37201 ctcagcataa cattttctcg cctgacttaa gagcgtgaaa aaatgcgtat gcgtcttgga
    37261 atgagccgaa acaaattcgt caatcgtaat attcagttta tccaaaagat taagcaagcg
    37321 actgcaggtg atttctgatt cccctcgttc aaatcttgaa atttgtgact tggacaggta
    37381 ttcatctgct aaaaaagaaa tgctaacctg cttaccctta cgtaatcgcc ttaaggtttt
    37441 tccgagttct ttttccattt ttcccacttt cacaacaaaa aattaaaatc tgctaaatcc
    37501 attatataat acaagtacaa aatagaaaat cagaaaggag acagtcatga aaaaagttaa
    37561 taaagctttg ctttttacat tgatcatgga tatcctgatt atcgttggtg gataaccttt
    37621 cgcttaaaaa caatccactt ttgggaaacc cttatattta gtttgtaaca ggaaaactaa
    37681 taaatattaa aaagagtgtt agcgacctag cggcagcttt actcttaagt gcgtttgctg
    37741 ccattgacag caaggtcaaa gctaatgaaa cagcaggagc ataccgttat gatgtgtctg
    37801 aacaaaacac ttactatcaa actatcagct ccattaacca gcaacgacct ggtgccactc
    37861 tcgacgaatg aggtgattat tatggaaccc ttacttcatt tacttcccaa aatcgtacca
    37921 gtacttatac acttggtaga caaagccaac gtatcatggt taacagtgat attacagtta
    37981 cctatactct taaagacaat ttggtagccc ttcataagga atataaaacc tttgtcaaaa
    38041 aaatattaca gataaaccga tcaccagtga ctacgacaag gctaaggtta tttataacca
    38101 tttactcaac gaatacactt acgccacaaa ataacttagc accaatcaca aaaccgtaag
    38161 cagcatcaac aatcatactc ctaaagctct ttatcgtggc aagtgtggtg tttgtcaagc
    38221 ttatgccgtc atgtttaaag atatggcaga agctgcaggc ttatcagctt ggtatgtgat
    38281 aaaaaaggat tgaatgagca ccatgcttga aacatcgtta ccattcatgg tgttaattcc
    38341 tacgttgatg ctacctggaa tgattcagca tacaccacaa ggtatttcct tgctggaaaa
    38401 gacgtgatga atactgagta ccgtttagat agccagtaca accacttggc taatactatt
    38461 ttaactacca gctaccaaaa aggctaaact aacaacaact acatttttca actagaaagg
    38521 cctaaacaat gaccaaaaaa cagttttatc ttatcttagc tattagcata ctgtgtttta
    38581 ttcttgccgt cagccctaaa ccaccaattc ctaaacacac tattgaaagg aaaaggactg
    38641 cgatgaccat aggccactca aaacaaaccc ctcacaaaaa agaagccctt aaacacaaaa
    38701 aaaacaaaaa taaggctcta tttcttccaa gtggggacag cgctatcaaa tctgttatta
    38761 gcatgacagt tagactcctg ttagatgacc aatattagct ctcctaaaat cagtggacac
    38821 taccatttcg gtagtgttct tttttaagct aatctcatca ccacgctttc ttcaagctaa
    38881 aaaactgatc atgaaagagc aaccccatca ccctaacagc ccactttgcc cgcactttct
    38941 taaaaagtta ggagaaaacg atataccaat tattttctga tcccagctcc ctatcattaa
    39001 caagatgtgt agcacggacc tacatacagg taagctagag tcattctcgc tacttcggac
    39061 aaaaagaggc cggtagttcc ctgacctctt ctttatttat cttgtaacac tcctcttctt
    39121 agcgagactt actagcacat aattcttaag cactcttatc atttagacta ggaataacac
    39181 catctgaatg aagcaaatga tgcgtcagat ctgccagagt atcatctcca taagtaatcc
    39241 ctgtttggtg ctgtttctga tgaaaatcag atgactcctt aactttagca gtcacctgct
    39301 ggtcacttgg atgaaaatta atttctctct tggcttcagc cgcaactggc tcgaggaaca
    39361 acttattgac attacggtct tcctccaaat gattaaggat agcagcatca tcttctaacg
    39421 aaagaacctt cttgggtaac aaaaaggggt tatttagcct tggctcagta acgacttcct
    39481 tggctactcg tgatagttta ttattgtctt cctcaggtat caaacggtta ggcaatcgat
    39541 gcaattcttc cggctatgag gcgctcacag ctacttcaga agaaacctcc aagccctttc
    39601 ctgtttgcct gtctaactaa agcaaccggc aaattattaa ctaacttttt aacctgtcct
    39661 cagcgctcat ttcaacacta ggaagagctg tcgctggttc tagccagcta ccaacttctg
    39721 ctggtcttac cttaacatcg ctggcaggct tctccttctc atcttcatta ggtctaaaag
    39781 gctcaaaccg ccctgttgat ttttcatcta tgacctttat attgtctttt actacatgag
    39841 acagtggttc gacatttacc atagcttcat gctcgttaag gctctggtct cgtttgaatt
    39901 taccaggaat accaggagtc ggtgttccga cagattgatc ggcttcagta ggctctttta
    39961 cggagccttt ttcccataac accgctgatg gaatgatatc cgtagagcca tctccgtaaa
    40021 taatcgtggc ctgaccacct cttggattaa tagatacctt aatattgtct gggaaagccc
    40081 cactacttcc tgtggttaaa agggcacccg catttttaat gagagttgtc attcgttcaa
    40141 taccagcaac gccaatatgg ttcaagtcat ctactggtaa ctgatcaaca gatttcatgg
    40201 tgtatttcca agcttgcggt tgaacaagaa aaacagttcc tttatacatt gtgttatgca
    40261 tatcatcaat gactttgaaa taccttgtat atttggtttg tacattaggg ttagcaacgg
    40321 gaacagtgct ttcaggaact cctgacatcg taaaaatccc tctagcagaa gtttgaaagc
    40381 cctcataaac atgacccata ccaggaacag tctgagcggt caaagactta ccagaaccat
    40441 ccaaagcaat gcgaatatct ttcatcagat acatctgatt ggtgtaggta aacctaaccg
    40501 tgttagcctc acctcgatac aaaaccactt gggagggctg atccgaagaa aactgagagg
    40561 ttgtttcttc cttatagcca actccaaaac gtctatcaga cgaatggact gaatctgccg
    40621 caactgtact actcaaactg actagtaaag tagccgcaca accaactaat aaactgctca
    40681 ttttaacttt aacatctctt aacatcatca tgttcctttc attatttttt atttaggaat
    40741 tcgtcatact atattttctc atattagtct aacaaaaaac ccgtaattct atataaatca
    40801 tataaaatga cgagctttgg caaagatgag tatagcagga tggaaaacag attgtttctg
    40861 aaacttcaaa ggtatcttct tgtcacgaac tattctttaa ttgaatcggt tacactaaac
    40921 tagtcccatt atttaaaaac aaactttaaa tatgattcat ttcacttt
//
LOCUS       DATXIG010000011        40185 bp    DNA     linear   BCT 28-DEC-2023
DEFINITION  TPA_asm: Streptococcus pyogenes strain MGAS11113
            SAMN08555799-rid19682583.denovo.011, whole genome shotgun sequence.
ACCESSION   DATXIG010000011 DATXIG010000000
VERSION     DATXIG010000011.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN08555799
            Sequence Read Archive: SRR6740495
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pyogenes
  ORGANISM  Streptococcus pyogenes
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 40185)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 40185)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-OCT-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-OCT-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001918058.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001918058.2
            Genome Coverage       :: 368x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/12/2023 11:23:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 1,806
            CDSs (total)                      :: 1,769
            Genes (coding)                    :: 1,722
            CDSs (with protein)               :: 1,722
            Genes (RNA)                       :: 37
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1, 1 (5S, 23S)
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 47
            CDSs (without protein)            :: 47
            Pseudo Genes (ambiguous residues) :: 0 of 47
            Pseudo Genes (frameshifted)       :: 30 of 47
            Pseudo Genes (incomplete)         :: 20 of 47
            Pseudo Genes (internal stop)      :: 9 of 47
            Pseudo Genes (multiple problems)  :: 11 of 47
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..40185
                     /organism="Streptococcus pyogenes"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN08555799-rid19682583.denovo.011"
                     /strain="MGAS11113"
                     /serotype="Emm28"
                     /isolation_source="normally sterile site"
                     /host="Homo sapiens"
                     /db_xref="taxon:1314"
                     /country="Finland"
                     /collection_date="2002"
                     /collected_by="National Public Health Laboratory"
     gene            137..298
                     /gene="sagA"
                     /locus_tag="VC122_000589"
     CDS             137..298
                     /gene="sagA"
                     /locus_tag="VC122_000589"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002985285.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TOMM family cytolysin streptolysin S"
                     /protein_id="HEP5640534.1"
                     /translation="MLKFTSNILATSVAETTQVAPGGCCCCCTTCCFSIATGSGNSQG
                     GSGSYTPGK"
     gene            520..1470
                     /gene="sagB"
                     /locus_tag="VC122_000590"
     CDS             520..1470
                     /gene="sagB"
                     /locus_tag="VC122_000590"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010922108.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="streptolysin S biosynthesis dehydrogenase SagB"
                     /protein_id="HEP5640535.1"
                     /translation="MSFFTKEQQPKENCPPITVEKARQLFEFNTNHLSLSDYHHQTVL
                     KTSKQLVAQHLMPNATDNLSQHFLMNYKANNNYLGFQASIVDFFTDSAVANFSSSYVY
                     ESQEKIIRLPKPTKISTALSTCIIKRRSHRQFSDRQMPLQDLSNILYYACGVSSQASI
                     RDGASDKITLRNCASGGGLYPIHLVFYARNISKLIDGFYEYLPYQHALRCYRHSSEEN
                     VRDFAEYGAINAENCNIIIIYVYHYMKNTRKYGNQATAYAFIESGEIAQNIQLTATAL
                     TYGSIDIGGYNKEYLQELLDLDGLGEHVIHMTLVGTKESQ"
     gene            1467..2525
                     /locus_tag="VC122_000591"
     CDS             1467..2525
                     /locus_tag="VC122_000591"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002985275.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="streptolysin associated protein SagC"
                     /protein_id="HEP5640536.1"
                     /translation="MKYQLNSNVRVVTFQDTFCFRKGIWDFNEAILDLTQEPQNLKIV
                     YQEIVSQLVKGVAIDTETYENTLEPEMFANLMEVISGLYYNDMLMLEDDYNLEENVMK
                     ILMGNFRFMAQAGHMVSNAPVLFISDSTYVNESAELLAEQLHLQLQVASDDLKMLIQQ
                     TDVSSRLDALEHRRNMNCLSEALCDYQSIIICQERLNIMMLRHLNEVSVAMKKQLVIG
                     FVDGPFLHTCTLNPPHSADFDSLERRVLARLQDSTLYQHFANQILPATQDVSQAYLPL
                     LNVLMNLVVSEAFIIAQTGSSKFEGRLLSIYLPTLEIQVQDILKMSNSQTQGALAKLK
                     YEDQQISTREIVKKLLDE"
     gene            2545..3903
                     /locus_tag="VC122_000592"
     CDS             2545..3903
                     /locus_tag="VC122_000592"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014407460.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YcaO-like family protein"
                     /protein_id="HEP5640537.1"
                     /translation="MLYYYPSFNHIFDELKSLSGNRTGILNQSQVPVCNHPHDVYLKS
                     ITGQMPDYHKQFIGELSQVSYHIIGYGSYYEEALIKYLGESIERYATVIAGDLLSDRI
                     VYASYNELKLLHKVMPLEYLQVFTQEQIALSCDLQMMMCDKMVTENDVLGWVKCPMFF
                     EDAEMYVPAQMLCVGYKTNETVGERRIIPGFSTGTASHKTLEAAMCNSLIEYIQIDSM
                     MLSWYTKKPCPKIIVDDPDIEVILEEARLGKDSLYDIIPIDMTVGEDNPLYTFGIILK
                     NKYDEGPYLLFGVQAGLDPKHALLRGIMEASAISYSYYYNLLYQKASLANIECEEPLF
                     LDLDSNVFYYAHPKDQDHKWKAFEPLISGEVLLSDLEDHSGKDKKEDLKTLLAYAKKV
                     SPNAVFLDITPPEALEKGWYVTRVLMPELLEMCIPAFPFANHPRMRQFGGVTNAFVHP
                     MP"
     gene            3878..4549
                     /locus_tag="VC122_000593"
     CDS             3878..4549
                     /locus_tag="VC122_000593"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011017602.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CPBP family intramembrane metalloprotease"
                     /protein_id="HEP5640538.1"
                     /translation="MPLSIQCLNLCFLLVTFCPSIPMQAIFGKEDSGYAFNLIGFLRA
                     TLIYDILALVSIYVLSPQITLSLESIDSKTFFMGLVFCVLIVLIELVFLHGLRCWQKK
                     QWLPATFSFVGTTNDWSKIGYPLLLALFEETIYRFLWFNILAFQWHLPTIIVLIVTSF
                     CYALNHLLMGKSIFYAKLVTGIIYGSIYMLTSQLWLVVIMHVGGNLLVECLSHLQTKK
                     KKGVT"
     gene            4546..5229
                     /locus_tag="VC122_000594"
     CDS             4546..5229
                     /locus_tag="VC122_000594"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002985270.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="streptolysin associated protein SagF"
                     /protein_id="HEP5640539.1"
                     /translation="MMLLVLLSFLGLTTLWLTSYRRRCLARWHLQWLVALSYQDFLDV
                     LLSLFQFVIIILVLFFYSATINLGEVLTFLTQTSWHWQILCYLVLYLMAIIEMTLLVL
                     ILIFDVLLQKDSRLLFKKITWLPFRQDKPVLSILLLGIAILVDSLFYLGLLILLGEQS
                     LTSLATLILGYGIVKACRYSGWLNVLLAFCLFTIVGLWAVTATLLYGWLVGAMILTVT
                     YLMISCKEY"
     gene            5252..6175
                     /locus_tag="VC122_000595"
     CDS             5252..6175
                     /locus_tag="VC122_000595"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011017604.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEP5640540.1"
                     /translation="MSFVQLTNVVKSYKNGKKAVNDVSLSIEAGNIYGLLGPNGAGKS
                     TLINLILGLIPLSSGKITVLGQSQKTIRKISSQIGYVPQDIAVYPDLTAYENVELFGS
                     LYGLKGAQLKKQVLKSLEFVGLHSQAKQFPSQFSGGMKRRLNIACALVHSPKLIIFDE
                     PTVGIDPQSRNHILESIRLLNKEGATVIYTTHYMEEVEALCDYIFIMDHGQVIEEGPK
                     FELEKRYVANLANQIIVTLTDSRHLELADKPDWSLIEDGEKLMLKIDNSDMTSVVHQL
                     TQANITFSEIRHNHLNLEEIFLHLTGKKLRD"
     gene            6184..7311
                     /locus_tag="VC122_000596"
     CDS             6184..7311
                     /locus_tag="VC122_000596"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002985266.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEP5640541.1"
                     /translation="MVLFHLIKKESLQIFRNRTALLMMVIFPILMIVILSFAFKSSFN
                     TATTVPKLTIRYQLEGEKTDYQKNFLAFLKVLNQKLHLEAKPSNSLEKDRQRVSEGAL
                     TAVLEVKKNQTIKVITNNINQQNADLINMLVKNYVDNAKTYDSIAALYPQQLNHIRKR
                     SVDYVKVSSIQTSKGMTSADYYAISMFTMITFYSMMSAMNLVLSDRQQRITNRIHLTG
                     VSPSLLVFGKLIGAMLATTVQLSLLYIFTRFVLRVNWGTNEWMLIGITASLVYLSVAI
                     GIGLGISIKNEAFLTVASNTIIPIFAFLGGSYVPLTTLHSSIINQLSNISPIKWVNDS
                     LFYLIFGGQYNPIPVTLIVNISIGTIFIILALIGMRKQVTT"
     gene            7308..8426
                     /locus_tag="VC122_000597"
     CDS             7308..8426
                     /locus_tag="VC122_000597"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002990425.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEP5640542.1"
                     /translation="MICFIKTLFVKIKRKKTSYVTFFLMPILTTLLALSLSFSNNNQA
                     KIGILDKDNSQISKQFIAQLKQNKKYDIFTKIKKEHIDHYLQDKSLEAVLTIDKGFSD
                     KVLQGKSQKLNIRSIANSEITEWVKAQTNYLLENYNIIGDVALGNEDTFNRILQKNQQ
                     LNYDVKQVTLTDRSRSKAVSSTTTGFLLILMLGSTSVIYSGILADKSGQLYHRLMLSN
                     LSRFRYMLSYVCVGFVAFTIQIVIMLSLLKVFNISFFVPTSLLLIIFFLFSLLAIGFG
                     LLIGAITQNSQQSSQLANLIVMPTSMLAGCLWPLSITPSYMQAIGKLLPQNWVLSAIA
                     IFQSGGTLSQAWPYLLALMGTALALISFSSLLLKPTKL"
     gene            8998..11730
                     /locus_tag="VC122_000598"
     CDS             8998..11730
                     /locus_tag="VC122_000598"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002985259.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SpnA family nuclease"
                     /protein_id="HEP5640543.1"
                     /translation="MINKKYIIPVSLLTLAITLTSVEEVTSRQNLTYANEIVTQRPKR
                     ESVISDKSNFPVISPYLASVDFGERKTSLPTPDKGVKVTTEQSIAQVRKGPEERTYTV
                     TGKITSVINGWGGYGFYIQDSEGIGLYVYPQKDLGYSKGDIVQLTGTLTRFKGDLQLQ
                     QVTAHKKLELSFPTSVKEAVISELETTTPSTLVKLSHVTVGELSTDQYNNTSFLVRDD
                     SGKSIVVHIDHRTGVKGADVVTKISQGDLINLTAILSIVDGQLQLRPFSLEQLEVVKK
                     VTSSNSDASSRNIVKIGEIQGASHTSPLLKKAVTVEQVVVTYLDDSTHFYVQDLNGDG
                     DLATSDGIRVFAKNAKVQVGDVLTISGEVEEFFGRGYEERKQTDLTITQIVAKAVIKT
                     GTAQVPSPLVLGKDRIAPANIIDNDGLRVFDPEEDAIDYWESMEGMLVAVDDAKILGP
                     MKNKEIYVLPGSSTRPLNNSGGVLLPANSYNTDVIPVLFKKGKQIIKAGDSYKGRLAG
                     PVSYSYGNYKVFVDDSKNMPCLMDGHLKPEKTNLQKDLSKLSIASYNIENFSANPSST
                     KDEKVKRIAESFIHDLNAPDIIGLIEVQDNNGPTDDGTTDATQSAQRLIDAIKKLGGP
                     TYRYVDIAPENNVDGGQPGGNIRTGFLYQPERVSLSDKPKGGARDALTWVNGELNLSV
                     GRIDPTNAAWKDVRKSLAAEFIFQGRKVVVVANHLNSKRGDNALYGRVQPVTFKSEQR
                     RHVLANMLAQFAKEGAKHQANIVMLGDFNDFEFTKTIQLIEEGDMVNLVNRHDISDRY
                     SYFHQGNNQTLDNILVSRHLLDHYEFDMVHVNSPFMEAHGRASDHDPLLLQLSFSKEN
                     DKAESSKQSVKAKKTSKGKLLPKTGDSLVYVITLLGTASLLVPILLLTKGKKES"
     regulatory      11895..12010
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF01054"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="PreQ1 riboswitch class II; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /bound_moiety="preQ1"
                     /db_xref="RFAM:RF01054"
     gene            12008..12508
                     /locus_tag="VC122_000599"
     CDS             12008..12508
                     /locus_tag="VC122_000599"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002985256.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="QueT transporter family protein"
                     /protein_id="HEP5640544.1"
                     /translation="MTKLTVHDYVHIGLVAALYVVLTITPPLNAISYGMYQFRISEMM
                     NFLAFYHRKYIIAVTLGCMIANFYSFGLIDVFVGGGSTLIFVTLGVILFSKYQKDYLF
                     NGIFNKAFVYFSFFFATSMFTVAIELYFFGAPFLLTWFTTALGELVSLLIGSLIIDKL
                     SQRISF"
     gene            12702..14660
                     /gene="ligA"
                     /locus_tag="VC122_000600"
     CDS             12702..14660
                     /gene="ligA"
                     /locus_tag="VC122_000600"
                     /EC_number="6.5.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011284658.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent DNA ligase LigA"
                     /protein_id="HEP5640545.1"
                     /translation="MKKRIKELTDLLNRYRYDYYTKDAPSVSDSDYDKLYRELVTLEQ
                     SYPEYVLQDSPTQQVGGTILKGFEKYRHQYPLFSLQDAFSREELDAFDKRVKAEFPNA
                     TYLAELKIDGLSISLSYENGFLQVGATRGDGNIGENITENIKKIKDIPHQLSEPLTIT
                     VRGEAYMSRQSFKAINEARQENGETEFANPRNAAAGTLRQLDTAVVAKRQLATFLYQE
                     ASPTARNQQNEVLAELADLGFSVNPYYQLTSSMDEIWNFIKTIEAKRDQLAYDIDGVV
                     IKVNSLAMQEELGFTVKAPRWAIAYKFPAEEKEAEILSVDWTVGRTGVVTPTANLTPV
                     QLAGTTVSRATLHNVDYIAEKDIRIGDTVIVYKAGDIIPAVLNVVMSKRNQQEVMLIP
                     KLCPSCGSELVHFEDEVALRCINPLCPSLIQRSLEHFASRDAMNITGLGPAIVEKLFL
                     AGFVHDVADIYQLTKEDFMQLDGIKEKSADKLLAAIEASKSNSAEKLLFGLGIRHIGS
                     KVSRLILEVYGDISALLTAKEEEIARIDGLGSTIAQSLTQYFEQKTAAILVDELKTAG
                     VNMHYSGQKVNSDAALFGLTVVLTGKLNQLNRNEAKDKLEALGAKVTGSVSKKTDLVI
                     AGSDAGSKLEKAKSLGIRIEDEDWLRQL"
     gene            14674..15696
                     /locus_tag="VC122_000601"
     CDS             14674..15696
                     /locus_tag="VC122_000601"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014612172.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diacylglycerol kinase family lipid kinase"
                     /protein_id="HEP5640546.1"
                     /translation="MKKQLRARLIYNPTSGQELMRKSVPEVLDILEGFGYETSAFQTT
                     AQKNSALNEARRAAKAGFDLLIAAGGDGTINEVVNGIAPLKKRPKMAIIPTGTTNDFA
                     RALKVPRGNPSQAAKLIGKNQTIQMDIGRAKKDTYFINIAAAGSLTELTYSVPSQLKT
                     MFGYLAYLAKGVELLPRVSNVPVKITHDKGVFEGQVSMIFAAITNSVGGFEMIAPDAK
                     LDDGMFTLILIKTANLFEIVHLLRLILDGGKHITDRRVEYIKTSKIVIEPQCGKRMMI
                     NLDGEYGGDAPITLENLKNHITFFADTDLISDDALVLDQDELEIEEIVKKFAHEVEDL
                     EQELEE"
     gene            16088..16285
                     /locus_tag="VC122_000602"
     CDS             16088..16285
                     /locus_tag="VC122_000602"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002985249.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit C"
                     /protein_id="HEP5640547.1"
                     /translation="MNPIFALALACFGVSLAEGFLMANLFKAASRQPEIIGQLRSLMI
                     LGVAFIEGTFFVTLVMAFILK"
     gene            16320..17036
                     /gene="atpB"
                     /locus_tag="VC122_000603"
     CDS             16320..17036
                     /gene="atpB"
                     /locus_tag="VC122_000603"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003047872.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit A"
                     /protein_id="HEP5640548.1"
                     /translation="MEEAKIPMLKLGPITFNLTLLAVCIVTIAVIFAFVFWASRQMKL
                     KPEGKQTALEYLISFVDGIGEEHLDHNLQKSYSLLLFTIFLFVAVANNLGLFTKLETV
                     NGYNLWTSPTANLAFDLALSLFITLMVHIEGVRRRGLVAHLKRLATPWPMTPMNLLEE
                     FTNFLSLAIRLFGNIFAGEVVTGLIVQLANYRVYWWPIAFLVNMAWTAFSVFISCIQA
                     FVFTKLTATYLGKKVNESEE"
     gene            17054..17548
                     /gene="atpF"
                     /locus_tag="VC122_000604"
     CDS             17054..17548
                     /gene="atpF"
                     /locus_tag="VC122_000604"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003047875.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit B"
                     /protein_id="HEP5640549.1"
                     /translation="MSITFGELVGNFILVTGSVIVLLLLIKKFAWGAIESILQTRSQQ
                     ISRDIDQAEQSRLSAQQLEAKSQANLDASRSQASKIISDAKEIGQLQGDKLVAEATDE
                     AKRLKEKALTDIEQSKSDAISAVKTEMSDLTVLLAEKIMGANLDKTAQSQLIDSYLDD
                     LGEA"
     gene            17548..18084
                     /locus_tag="VC122_000605"
     CDS             17548..18084
                     /locus_tag="VC122_000605"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003057492.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit delta"
                     /protein_id="HEP5640550.1"
                     /translation="MTKKEQALIEQYAKSLVEVASEHHSLDALQADVLAILETFVTTN
                     LDQSLSSLAVPHAEKIKLLTLLKGNNSVYMNNFLNLILQNEREAYLYQMLQTVLNEIA
                     IVSNQYDVTVTSSLPLTEEQKSRVRAVVAKKFAVTAGRLIEKVDPSLIGGFIISVNNK
                     VIDTSIRRQLQAFKMNLK"
     gene            18100..19608
                     /locus_tag="VC122_000606"
     CDS             18100..19608
                     /locus_tag="VC122_000606"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002994422.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit alpha"
                     /protein_id="HEP5640551.1"
                     /translation="MAINAQEISALIKKQIENFQPNFDVTETGIVTYIGDGIARARGL
                     DNAMSGELLEFENGAYGMAQNLESNDVGIIILGDFSAIREGDVVKRTGKIMEVPVGEA
                     LIGRVVNPLGQPVDGLGDIETTGFRPVETPAPGVMQRKSVSEPLQTGLKAIDALVPIG
                     RGQRELIIGDRQTGKTSVAIDAILNQKGQDMICIYVAIGQKESTVRTQVETLRRYGAL
                     DYTIVVTASASQPSPLLFIAPYAGVAMAEEFMYQGKHVLIVYDDLSKQAVAYRELSLL
                     LRRPPGREAYPGDVFYLHSRLLERSAKVSDDLGGGSITALPFIETQAGDISAYIATNV
                     ISITDGQIFLQENLFNSGIRPAIDAGSSVSRVGGSAQIKAMKKVAGTLRLDLASYREL
                     EAFTQFGSDLDAATQAKLNRGRRTVEILKQPLHKPLPVEKQVVILYALTHGFLDDVPV
                     DDILAFEEALYDYFDVHYNDLFETIRTTKDLPEEAALDAAIKAFKEHSNFKS"
     gene            19624..20499
                     /locus_tag="VC122_000607"
     CDS             19624..20499
                     /locus_tag="VC122_000607"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000919083.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit gamma"
                     /protein_id="HEP5640552.1"
                     /translation="MAGSLSEIKAKIISTEKTSKITSAMRMVSSAKLVKSEQAARDFQ
                     IYASKIRQITTDLLKSELTIGSDNPMLVSRPVKKTGYIVITSDKGLVGGYNSKILKSV
                     MDMITEYHADGDYEIISIGSVGSDFFKARGMNVAFELRGLADQPSFEQVRQIISQSVD
                     MFVNEIFDELYVCYNHHVNSLTSQVRVQQMLPISDLVADEAAEEGVTGFELEPNRHDI
                     LDQLLPQFTESLIYGAIIDAKTAEHAAGMTAMQTATDNAKNVINDLTIQYNRARQAAI
                     TQEITEIVAGANALE"
     gene            20661..22067
                     /gene="atpD"
                     /locus_tag="VC122_000608"
     CDS             20661..22067
                     /gene="atpD"
                     /locus_tag="VC122_000608"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017643977.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit beta"
                     /protein_id="HEP5640553.1"
                     /translation="MSSGKIAQVVGPVVDVMFASGDKLPEINNALIVYKDSDKKQKIV
                     LEVALELGDGMVRTIAMESTDGLTRGLEVLDTGRAISVPVGKETLGRVFNVLGETIDL
                     EEPFAEDVDRQPIHKKAPSFDELSTSSEILETGIKVIDLLAPYLKGGKVGLFGGAGVG
                     KTVLIQELIHNIAQEHGGISVFTGVGERTREGNDLYWEMKESGVIEKTAMVFGQMNEP
                     PGARMRVALTGLTIAEYFRDVEGQDVLLFIDNIFRFTQAGSEVSALLGRMPSAVGYQP
                     TLATEMGQLQERITSTQKGSVTSIQAIYVPADDYTDPAPATAFAHLDSTTNLERKLTQ
                     MGIYPAVDPLASSSRALSPEIVGEEHYAVATEVQRVLQRYRELQDIIAILGMDELSDE
                     EKTLVGRARRIQFFLSQNFNVAEQFTGLPGSYVPVAETVRGFKEILEGKYDDLPEDAF
                     RSVGPIEDVIKKAEKMGF"
     gene            22080..22496
                     /locus_tag="VC122_000609"
     CDS             22080..22496
                     /locus_tag="VC122_000609"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003088947.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit epsilon"
                     /protein_id="HEP5640554.1"
                     /translation="MTQMTVQVVTPDGIKYDHHAKCISVTTPDGEMGILPNHINLIAP
                     LQVHEMKIRRGGEDEKVDWIAINGGIIEIKDNVVTVVADSAERDRDIDVSRAERAKLR
                     AEREIAQAETTHNIDEVRRAKVALRRALNRINVSKK"
     gene            22762..23019
                     /locus_tag="VC122_000610"
     CDS             22762..23019
                     /locus_tag="VC122_000610"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002994426.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1146 domain-containing protein"
                     /protein_id="HEP5640555.1"
                     /translation="MVCYNNPMELLMKIIAFSCHLFFIGLSYQLLISVIDWTKFSHYH
                     PENLGRLRLFVFLLATALGYLVSHCMLELLQISQTLFFEFR"
     gene            23084..24355
                     /gene="murA"
                     /locus_tag="VC122_000611"
     CDS             23084..24355
                     /gene="murA"
                     /locus_tag="VC122_000611"
                     /EC_number="2.5.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002994428.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylglucosamine
                     1-carboxyvinyltransferase"
                     /protein_id="HEP5640556.1"
                     /translation="MDKIIIEGGQTRLEGEVVIEGAKNAVLPLLAASILPSKGKTILR
                     NVPILSDVFTMNNVVRGLDIRVDFNEAANEITVDASGHILDEAPYEYVSQMRASIVVL
                     GPILARNGHAKVSMPGGCTIGSRPINLHLKGLEAMGATITQKGGDITAQADRLQGAMI
                     YMDFPSVGATQNLMMAATLADGVTTIENAAREPEIVDLAQFLNKMGARIRGAGTETLT
                     ITGVTSLHGVEHDVVQDRIEAGTFMVAAAMTSGNVLIRDAVWEHNRPLISKLMEMGVS
                     VTEEEYGIRVQANTPKLKPVTVKTLPHPGFPTDMQAQFTALMAVVNGESTMVETVFEN
                     RFQHLEEMRRMGLQSEILRETAMIHGGRQLQGAPVMSTDLRASAALILTGIVAQGVTI
                     VNNLVHLDRGYYQFHEKLAKLGATISRSSEV"
     gene            24359..24547
                     /locus_tag="VC122_000612"
     CDS             24359..24547
                     /locus_tag="VC122_000612"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002985226.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit beta"
                     /protein_id="HEP5640557.1"
                     /translation="MTNGLKYVLEQMLLLLVIAALACLFLAIGLMIGYSFMGDGHSPW
                     HILSMDKWAELVNKFTGK"
     gene            24583..25447
                     /locus_tag="VC122_000613"
                     /pseudo
     CDS             24583..25447
                     /locus_tag="VC122_000613"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011184363.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DNA/RNA non-specific endonuclease"
     gene            25730..26773
                     /gene="pheS"
                     /locus_tag="VC122_000614"
     CDS             25730..26773
                     /gene="pheS"
                     /locus_tag="VC122_000614"
                     /EC_number="6.1.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019314825.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phenylalanine--tRNA ligase subunit alpha"
                     /protein_id="HEP5640558.1"
                     /translation="MDLQAQLEELKTKTLETLQSLTGNHTKELQDLRVAVLGKKGSLT
                     ELLKGLKDLSNDLRPVVGKQVNEVRDLLTKAFEEQAKIVEAAKIQAQLDAESIDVTLP
                     GRQMTLGHRHVLTQTSEEIEDIFLGMGFQIVDGFEVEKDYYNFERMNLPKDHPARDMQ
                     DTFYITEEILLRTHTSPVQARTLDQHDFSKGPLKMVSPGRVFRRDTDDATHSHQFHQI
                     EGLVVGKNISMGDLKGTLEMIIKKMFGEERSIRLRPSYFPFTEPSVEVDVSCFKCGGK
                     GCNVCKKTGWIEILGAGMVHPSVLEMSGVDAKEYSGFAFGLGQERIAMLRYGINDIRG
                     FYQGDQRFSEQFN"
     gene            26983..29388
                     /locus_tag="VC122_000615"
     CDS             26983..29388
                     /locus_tag="VC122_000615"
                     /EC_number="6.1.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002311998.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phenylalanine--tRNA ligase subunit beta"
                     /protein_id="HEP5640559.1"
                     /translation="MLVSYKWLKELVDIDVTPAALAEKMSATSIEVEGIEVPADGLSK
                     LVVGHVLSCEDVPETHLHLCQVDTGDETPRQIVCGAPNVKAGIKVIVAVPGARIADNY
                     KIKKGKIRGMESLGMICSLQELGLSDSIIPKEFSDGIQILPEEAVPGDAIFKYLDLDD
                     HIIELSITPNRADALSMRGVAHEVAAIYGKSVSFPQKNLQESDKATSEAIEVAIASDN
                     VLTYASRVVENVKVKPSPQWLQNLLMNAGIRPINNVVDVTNYVLLYFGQPMHAFDYDK
                     FEDHKIVARAARQGESLVTLDGEKRDLTTEDLVITVADKPVALAGVMGGQATEIDANS
                     QTVVLEVAVFDGKSIRKTSGRLNLRSESSSRFEKGVNYATVLEALDFAAAMLQELAEG
                     QVLSGHVQAGQLPTEPVEVSTSLDYVNVRLGTELTFADIQRIFDQLGFGLTGDETSFT
                     VAVPRRRWDVSIPADLVEEIARIYGYDKLPTTLPEAGGTAAELTPTQALRRKVRGLAE
                     GLGLTEIISYALTTPEKAVEFAVAPSHLTELMWPMSVERSALRQNMVSGMLDTVAYNV
                     ARKQSNLALYEIGKIFEQEANPKEDLPNELNHFAFAICGLVAQKDFQTQAQAVDFYHA
                     KGILDTLFANLNLKVQYVPTKDLASMHPGRTALILLDEQVIGFVGQVHPGTAKAYSIP
                     ETYVAELDMAALEAALPSDQTFAEITKFPAMTRDVALLLDREVSHQAIVTAIESAGVK
                     RLTKIKLFDVYEGVTIQAGKKSMAYSLTFQNPNDNLTDEEVAKYMEKITKALTEQVGA
                     EVR"
     gene            29498..29875
                     /locus_tag="VC122_000616"
     CDS             29498..29875
                     /locus_tag="VC122_000616"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002985212.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="OsmC family protein"
                     /protein_id="HEP5640560.1"
                     /translation="MYQTKITGNRLYHTVSKGYGDSVTLFGKTDQGETPMSLLVIALS
                     SCVTMCVQGFYHRQFQQDTLAVSVEASYEEEVFELLVRIDDVLDDKKKADLLAYIGTY
                     CRVKQLLKPEVMVTITLEGLSHV"
     gene            29868..30254
                     /locus_tag="VC122_000617"
     CDS             29868..30254
                     /locus_tag="VC122_000617"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015016875.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YbgA family protein"
                     /protein_id="HEP5640561.1"
                     /translation="MSDAGLKTYQKQWAYQKYWVMAHSQQHYNALRELFKGNQWSEEK
                     VLTFHCLIEEAQAIPPTVKSLRTAYQHVWGYFKKVASQEEKDHFKDLDAQLETKSEEM
                     LCFLQEMTAHYQPSYLLSCRLITKGP"
     gene            30327..31403
                     /locus_tag="VC122_000618"
     CDS             30327..31403
                     /locus_tag="VC122_000618"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002993030.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEP5640562.1"
                     /translation="MFLALNEMKQSKLRYGLIAGLLCLVAYLMFFLSGLAFGLMQENR
                     SAVDLWKADSILLAKDADATLTLSQVSRAQENQITADKVAPLAQLNTVAWSVKNPKDA
                     DKVKVSLFGIDSNSFIRPNIVKGRLFKTNKEVVLDQSLAKEEAFAIGKDFYTSSSSQA
                     LTIVGYTQNARFSVAPVVYMNLEAFETLKYGEPLPKDKQVVNAFITKGSLTDYPKKDF
                     QKLDIKTFITKLPGYSAQLLTFGFMISFLVIISAIIIGIFMYILTIQKAPIFGIMKAQ
                     GISNKTITTAVLMQTFFLSFLGSGLGLLGTWLTSLLLPTVVPFQSNWFLYLAIFVSMI
                     CFALLGTLFSVFNIIRIDPLKAIG"
     gene            31413..32081
                     /locus_tag="VC122_000619"
     CDS             31413..32081
                     /locus_tag="VC122_000619"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011889019.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEP5640563.1"
                     /translation="MSVLTFKQVTKTFQDGHHEINALKATDFSIEAGEFVAIIGPSGS
                     GKSTFLTIAGGLQTPSSGQLIIDGTDYTHLSEKERSRLRFKSVGFILQASNLIPFLTV
                     QQQLELVDHLTGSKEKAKANQLFDDLGITGLKHQLPQELSGGERQRAAIARALYHDPA
                     LILADEPTASLDTEKAYEVVKLLAKESKEKNKAIIMVTHDDRMLKYCDKVYRMQDGEL
                     RQER"
     gene            complement(32186..33103)
                     /locus_tag="VC122_000620"
     CDS             complement(32186..33103)
                     /locus_tag="VC122_000620"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002990401.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="neutral zinc metallopeptidase"
                     /protein_id="HEP5640564.1"
                     /translation="MKTDDLRESQQVEDRRGQSSGSFGGGGLGGGLLLQLLFSRGGWK
                     TKLVILLLLLVMGGGGLSSVLGGKPSSTNNNAYQSSQVTRTNGDKASQEQVSFVSKVF
                     ASTEDYWTKTFREKGLTYHKPTLVLYTGATQTACGRGQASSGPFYCPGDQKVYLDISF
                     YNELSTKYGAKGDFAMAYVIAHEVGHHIQNELGIMDNYASARQGKSKAKANQLNVKLE
                     LQADYYAGAWANYVQGQGLLEKGDIEEAMAAAHAVGDDTLQEETYGRTVPDSFTHGTS
                     KQRQRWFDRGYQYGDFEHGDTFSIPYSKL"
     gene            33254..36469
                     /gene="rexB"
                     /locus_tag="VC122_000621"
     CDS             33254..36469
                     /gene="rexB"
                     /locus_tag="VC122_000621"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011285475.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent nuclease subunit B"
                     /protein_id="HEP5640565.1"
                     /translation="MKLIYTEMSYSMTEILVNEARKAADQGYRVFYIAPNSLSFEKER
                     EVLTLLPERGTFSIIVTRFVQMSRYFTVESSPSKQHLDDTTLAMIFYRALMQLKPEDL
                     PSYGRLQNNSVFIEQLVELYKELKNAQLSVHDLTGLDHPQKQEDLIKIIELAETIMIQ
                     QDYNQDSPLQSFARAIKLGLLNNQLSKTVIVIDGFSRFSAEEDYLLSLLNNNCQEVII
                     GSYVSQKAYQKSFIKGNIYEASLHFLQDLAQKYHIKPVFATSNQVFKPAFSRLTQLFE
                     ATHDFSQVDWQLQKNDLDHFSLWQCHHQKEEIEHVAKSIRQKLYEGYRYKDILVLLGD
                     MDAYQLQIGPIFDKFEIPYYLGKAEPMAAHPLVQFIESLERSQRYNWRREDILNMLKS
                     GLFGCFDDSDIDRFEEYTQFADIKGFTKFSKPFTINSSRQYPLDFLNEMRQDIVLPLQ
                     ELFKSQKQLGASLVDKLILFFKKIRLAENMQGLAQSQLEVEKNEEVWKRFTDILTSFH
                     HIFGQEKLRLSDCLALIKTGMKSAQYRVVPATLDVVTIKSYDLVQPHSKPFVYAIGLT
                     QSHFPKQIHHSGLLSDQERARINEIRNYRHFDIASAENSKKNHQTALSLFNAATKELV
                     LSVPTVINETFDDLSPYLKELISFGLPLLDKGKNYLSYDNSDIGNYKALLSQIIAINR
                     QDLIEMSDQDKMFWTVVLRYLRKQLRKQQLELPTSDYRLSTKPLSKEVIEVCFPKGIP
                     LKLSATALTVFYNNQYNYFLKYVLNLNKTESIHPDSRIHGQYLHRVFERLMKDHTQEP
                     FDNKLKQAIYHTNQESFFQQVYQDNAEAEYSLAILEDIVRSTAPILQLNQNIKVIDQE
                     KNFHLDMGNEILVHGIIDRIDQLSDGSLGIVDYKSSANQFDIGTFYNGLSPQLVTYLA
                     ALKQIAPHDINQLFGAMYLHLQDPKLDLVTFKQIDNTLVESIYKALTYKGIFSEVEKE
                     HLSTGAYQTKNALYSNDELETLLNYNKYLYLKAAKHIKKGHFLINPYTSDGKTVQGDQ
                     LKAITRFEADLDMAQARRLVTLPAKEKKECFLTLMRKESHL"
     gene            36430..40098
                     /gene="addA"
                     /locus_tag="VC122_000622"
     CDS             36430..40098
                     /gene="addA"
                     /locus_tag="VC122_000622"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020905049.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helicase-exonuclease AddAB subunit AddA"
                     /protein_id="HEP5640566.1"
                     /translation="MLFNINEKGEPLVISFAPFLSPEAIKHLQENERCSDQSQKRTAQ
                     QIEAIYTSGQNILVSASAGSGKTFVMVERILDKILRGVSIDRLFISTFTVKAATELRE
                     RIENKLYSQIAQTTDFQMKVYLTEQLQSLGQADIGTMDAFAQKVVSRYGYSIGISSQF
                     RIMQDKAEQDVLKQEVFSKLFSEFMNQKEAPVFRALVKNFSGNCKDTSAFRELVYTCY
                     SFSQSTENPKIWLQENFLSAAKTYQRLEDIPDHDIELLLLAMQDTANQLRDVTDMEDY
                     GQLTKAGSRSAKYTKHLTIIEKLSDWVRDFKCLYGKAGLDRLIRDVTGLIPSGNDVTV
                     SKVKYPVFKTLHQKLKQFRHLETILMYQKDCFPLLEQLQDFVLAFSEAYLAVKIQESA
                     FEFSDITHFAIKILEENTDIRQSYQQHYHEVMVDEYQDNNHMQERLLTLLSNGHNRFM
                     VGDIKQSIYRFRQADPQIFNQKFRDYQKKPEQGKVILLKENFRSQSEVLNVSNAVFSH
                     LMDESVGDVLYDEQHQLIAGSHAQTVPYLDRRAQLLLYNSDKDDGNAPSDSEGISFSE
                     VTIVAKEIIKLHNDKGVPFEDITLLVSSRTRNDIISHTFNQYSIPIVTDGGQQNYLKS
                     VEVMVMLDTLRTINNPRNDYALVALLRSPMFAFDEDDLARIALQKDNELDKDCLYDKI
                     QRAVIGRGAHPELIHDTLLGKLNIFLKTLKSWRRYAKLGSLYDLIWKIFNDRFYFDFV
                     ASQAKAEQAQANLYALALRANQFEKSGYKGLYRFIKMIDKVLETQNDLADVEVAAPKQ
                     AVNLMTIHKSKGLQFPYVFILNCDKRFSMTDIHKSFILNRQHGIGIKYLADIKGLLGE
                     TTLNSVKVSMETLPYQLNKQELRLATLSEQMRLLYVAMTRAEKKVYFIGKASKSKSQD
                     ITDPKKLGKLLPLALREQLLTFQDWLLAIADIFSTEDLYFDVRFIEDSDLTQESVGRL
                     QTPQLLNPDDLKDNRQSETIARALDMLEAVSQLNANYEAAIHLPTVRTPSQLKVAYEP
                     LLEPIGVDIIEKSSRSLSDFTLPHFSKKVKVEASHIGSALHQLMQVLPLSKPINQQTL
                     LDALREIDSNEEVKTALDLKKIESFFCDTSLGQFFQTYQKHLYREAPFAILKVDPISQ
                     EEYVLRGIIDAYFLFDDHIVLVDYKTDKYKQPIELKKRYQQQLELYAEALTQTYKLPV
                     TKRYLVLMGGGKPEIVEV"
BASE COUNT        12565 a         6834 c         8216 g        12570 t
ORIGIN      
        1 ctagatagtt gttgtgttac aacagtacaa ttgagctagc cttgtccttg ttgtgttaac
       61 tttattttta aaataaggtt aaaaataaac gactcgcgtt cttatcagtt acttattaga
      121 taaggaggta aaccttatgt taaaatttac ttcaaatatt ttagctacta gtgtagctga
      181 aacaactcaa gttgctcctg gaggctgctg ttgctgctgt actacttgtt gcttctcaat
      241 tgctactgga agtggtaatt ctcaaggtgg tagcggaagt tatacgccag gtaaataatc
      301 tatttagcat ctctatgtga tagtgatatt aaggtaatga gtttgctagc aactaagtta
      361 tctttggtaa cttctgatta tagatgaatg gcatagaggt gttagaaaac atgagacaaa
      421 agtaatcttc ttgatgctaa cgtctcaggt aattagcagg tactagatag tacctgctaa
      481 ttactatatg tttagtaaaa tgagatagga aagatagtta tgtcattttt tacaaaggaa
      541 caacaaccta aagagaattg tccgccaata actgttgaaa aagcaaggca attgtttgaa
      601 tttaatacaa accacttgtc cttatcggat taccatcatc aaacggtgct aaaaacgtca
      661 aagcagctag ttgctcaaca tttaatgcct aatgcaaccg ataatcttag tcaacatttt
      721 ttgatgaact ataaagctaa taataattat ttaggcttcc aagctagtat tgtcgacttt
      781 tttacagatt ctgccgttgc taatttttca agtagttacg tttatgaaag tcaggaaaag
      841 ataattcgtt taccaaaacc taccaagata tcaactgctc tgtcgacatg tattataaaa
      901 cgaagaagtc atcgtcaatt ttcagataga caaatgcctc ttcaagattt atcaaacatt
      961 ctttattatg catgtggtgt tagttcacaa gcatcaatta gagatggagc atcagataag
     1021 attacactca gaaactgtgc ttcaggtgga ggtttatacc ctattcattt agttttttat
     1081 gctagaaaca tcagtaaatt aatagatggt ttctatgaat atctacccta tcagcatgca
     1141 ctaaggtgtt atcggcatag ctctgaggaa aacgttagag attttgcgga atacggtgct
     1201 attaatgctg aaaattgtaa tattattatt atttatgtct accattacat gaaaaataca
     1261 cgtaaatatg ggaatcaggc gactgcctat gcttttattg aatcaggaga aatagcccag
     1321 aatattcaat tgactgcaac tgccttaact tatggaagta ttgatattgg tggttataat
     1381 aaggaatatc tccaagaatt attagattta gatgggctag gagagcatgt gattcacatg
     1441 acactcgtag gaactaagga gtctcaatga aatatcaact taatagtaat gttcgtgtcg
     1501 tgacatttca agatacattt tgttttagaa aaggaatctg ggattttaat gaagccattc
     1561 tagatttgac acaagaacct caaaatttaa agatagttta tcaagaaata gtttcacagt
     1621 tggttaaggg agtagccatt gatactgaaa cttatgaaaa tactttagaa cctgagatgt
     1681 tcgctaattt aatggaagtc atttcaggct tgtattacaa tgatatgctg atgcttgagg
     1741 atgactacaa tcttgaagaa aatgtcatga aaattttaat gggtaatttc cgttttatgg
     1801 cgcaagcagg tcatatggta agcaatgctc ctgttttgtt tatcagtgat tcgacttatg
     1861 ttaatgaatc tgctgagttg ctagctgaac aactgcattt acaattgcaa gtggctagcg
     1921 atgacttaaa aatgttaatt cagcagacag atgttagttc gcgtttagat gctttggagc
     1981 atcgccgtaa catgaattgt ttatcagaag ccttgtgcga ttaccaaagt attattattt
     2041 gtcaagaacg cttgaatatt atgatgttgc gacacttgaa tgaagttagc gtagctatga
     2101 aaaaacaatt agtgattggt tttgtagatg gaccattctt gcacacttgt actttgaatc
     2161 ctcctcatag tgctgacttt gatagcttag aaagaagagt tttggcacgt ttacaagact
     2221 caactctcta tcaacatttt gctaatcaga ttttaccagc aacacaagat gtgagccagg
     2281 cctatttacc attgttaaat gttttaatga atttagtcgt tagcgaagct ttcattattg
     2341 ctcaaacagg tagttctaaa tttgagggac gtttattaag catttactta ccgacgcttg
     2401 agattcaggt ccaagatatt ttgaaaatgt ctaattcaca gacacaagga gctttggcaa
     2461 aattaaaata tgaggatcag cagatatcaa cacgtgagat tgttaaaaaa ctccttgacg
     2521 agtaacctat aaggagaatc tattatgtta tactattatc cttcttttaa tcatattttt
     2581 gatgaattaa aaagtttaag tggcaatcga acaggtatct taaatcaatc tcaagttcca
     2641 gtttgcaacc atcctcacga tgtttactta aaaagtatta ctggccaaat gccagattat
     2701 cataaacaat ttataggtga gttgagtcaa gttagttatc acattattgg ttacggcagt
     2761 tactatgaag aagcacttat caagtattta ggagaaagta ttgaacgcta tgctactgta
     2821 attgccggag acttactatc agatcggatt gtctatgctt cttataatga gttaaaactg
     2881 ttacataaag tgatgccttt agaatacttg caagtgttta ctcaagaaca gatcgcatta
     2941 tcttgtgact tgcaaatgat gatgtgcgat aaaatggtaa ctgagaacga tgtattgggc
     3001 tgggttaaat gtcccatgtt ctttgaggac gcagagatgt atgttccagc tcaaatgctt
     3061 tgtgttgggt ataaaacaaa tgagactgta ggagaaagac ggataatacc aggattttct
     3121 acgggaacag cttctcacaa aacgttagaa gcggctatgt gcaacagttt gattgaatac
     3181 atccagatcg attcaatgat gcttagctgg tatactaaga agccttgccc caagattatt
     3241 gttgatgatc ctgatattga agtgatttta gaagaagcta gactgggaaa agacagtcta
     3301 tacgacatta ttcctattga tatgactgta ggagaagata atcctcttta tacgtttgga
     3361 atcattctca aaaataaata tgatgaaggg ccttacctct tgtttggagt gcaggctggg
     3421 ttagatccta aacatgcttt gctaagaggg atcatggaag cttcagctat tagctatagt
     3481 tactactata atcttttata tcaaaaagct tcccttgcaa atattgaatg cgaagaacct
     3541 ttgtttttag atttagatag taatgtcttc tattatgcac atcccaagga ccaagaccat
     3601 aaatggaagg cctttgaacc tttgatttca ggagaagttc tgttaagtga tttggaagat
     3661 cattctggta aagataaaaa agaagatttg aagactttat tagcttatgc taagaaagtc
     3721 agtcctaatg ctgtcttttt agatattaca cctcctgaag ctctagaaaa aggctggtac
     3781 gtcacgcgag ttctgatgcc agaacttctt gaaatgtgta taccagcatt tccttttgct
     3841 aatcatccac gaatgagaca gtttggaggt gtaacaaatg cctttgtcca tccaatgcct
     3901 taatctttgt tttttgttgg taactttttg tccttcaatt cctatgcaag ctatttttgg
     3961 aaaggaagat agtggttatg catttaattt aattggtttt ctaagagcaa ctctgattta
     4021 tgacattttg gctttggtaa gtatttatgt tttgtcacct caaataacct tgtctcttga
     4081 aagtattgat agtaagacgt tctttatggg acttgtattt tgcgtcttga tagtactgat
     4141 tgaactcgtt ttcttacatg gtttacgttg ttggcaaaaa aagcaatggc ttcctgcaac
     4201 tttctcgttt gtaggaacga caaatgattg gtctaagatt ggctatcctt tattactagc
     4261 tttatttgaa gagacgattt atcgtttttt gtggtttaat atattagctt ttcaatggca
     4321 tttaccaaca attattgttt taattgtaac tagtttttgc tatgctttaa atcacttatt
     4381 gatggggaaa tccatttttt atgctaagtt ggtaacaggc atcatttatg gtagtattta
     4441 catgctgact agtcaattgt ggttggttgt gataatgcat gtaggaggga acttgctagt
     4501 tgagtgtctt agccatcttc aaacaaaaaa gaagaagggg gtgacatgat gctattggtt
     4561 ttgctgtcgt ttttaggtct aactactctt tggttgacca gttatcgaag acgttgtttg
     4621 gctagatggc atcttcaatg gttagtggcc ttatcctatc aagatttttt agatgtctta
     4681 ctgagtctgt ttcaatttgt cattattata cttgttttat tcttttactc agctactatt
     4741 aatctaggag aggttctgac ctttttgaca caaactagct ggcattggca gattctttgt
     4801 tatttagtgt tgtatcttat ggccatcata gagatgacgc ttttagtttt aattcttatc
     4861 tttgatgttt tgctccagaa agatagtcga ttgctcttta aaaaaataac ttggctgcct
     4921 tttagacaag ataaaccagt cttaagtata cttttactag gcatagctat tctagtagac
     4981 agtctctttt atttgggctt gcttatatta ttaggagaac agtcgctaac aagtttagca
     5041 acgcttattt taggttatgg gatagtcaag gcttgtcgct attcaggctg gttaaatgta
     5101 ctattagctt tttgtctttt cactatagtt ggactatggg cagttacggc aactttatta
     5161 tatggttggc tagtaggtgc gatgatatta acggtgactt atttgatgat tagttgcaaa
     5221 gagtattagc aatattaaaa aggagtgata tatgagtttt gtacaattaa caaatgttgt
     5281 caagtcctac aaaaacggca agaaagctgt caatgatgtt tccttgtcta ttgaagcagg
     5341 taatatttat ggtctgttag gaccaaatgg tgctggtaag tctaccttga ttaatcttat
     5401 cttaggcttg atccctttga gttccggtaa aattactgtt ttagggcaat cccaaaagac
     5461 tattcgaaaa ataagttcgc agataggtta tgttcctcaa gacattgctg tttatccaga
     5521 cctaactgct tatgaaaatg tagaactatt tgggtcactt tatggcttaa agggagctca
     5581 gcttaaaaaa caagttctaa aaagtttaga atttgtgggg ctacactccc aagctaagca
     5641 gtttccaagt caattctcag gaggaatgaa gagacggtta aatatcgctt gtgcgctagt
     5701 tcattcacct aaattaatca tttttgacga accgactgta gggattgatc ctcaatcacg
     5761 taatcatatt ttagagtcga ttcgtttgct aaacaaagaa ggtgccacag ttatttatac
     5821 gacccattac atggaagaag tagaggctct ttgtgattat atttttatta tggatcatgg
     5881 tcaagttatt gaagaaggac ctaaatttga actggaaaaa cgttacgttg caaatctagc
     5941 aaaccagatt attgtaactc taacagattc acgtcatttg gaactggcag ataagcctga
     6001 ttggtctttg atagaagatg gagaaaaact catgttgaag attgataata gtgatatgac
     6061 atcagttgtt catcagctca cacaggccaa tattactttt agcgagatta gacataacca
     6121 tttgaattta gaagaaattt tcttacactt aacaggtaag aagttacgag attagaggtt
     6181 actatggttt tatttcattt aatcaaaaaa gaaagtttac agatttttag aaaccgaaca
     6241 gccttattga tgatggtgat ttttccaatt ttgatgatcg ttattttaag ttttgccttt
     6301 aaatcaagtt ttaatactgc gacaacagtc cctaaattga ccattcgtta ccaattagag
     6361 ggtgaaaaaa cggattacca gaaaaatttt cttgcttttt taaaagtttt aaaccaaaaa
     6421 cttcatttag aggctaaacc tagtaattct cttgaaaaag atcgacaaag ggtcagtgaa
     6481 ggagccttaa cggctgtttt agaagtgaag aagaatcaga ccattaaggt tattactaat
     6541 aatattaatc agcaaaatgc agatttgatc aatatgctag taaaaaatta tgtagataat
     6601 gctaaaactt atgactcgat agcagctctt tatcctcaac aattaaatca tatcagaaag
     6661 cgaagtgtgg actatgttaa ggtcagttca atacagacaa gtaaaggaat gacatcagct
     6721 gattattatg ctatttctat gtttaccatg attacttttt atagtatgat gtctgcgatg
     6781 aaccttgttt tgtcagatcg tcaacaacgg attacaaatc gtattcactt aacaggagtt
     6841 tctccaagtc ttttggtctt tggaaaatta ataggtgcta tgttagcaac aactgttcaa
     6901 ttgagtcttt tatacatttt tacaaggttt gttttacgag ttaattgggg cactaatgag
     6961 tggatgctta ttggtataac agcctcctta gtctatctct ctgtagctat aggtatcggg
     7021 ctaggcataa gcattaaaaa tgaggctttt ttaacggttg catccaatac tattattcct
     7081 atattcgcct ttttaggagg cagttacgtt ccattgacaa cattacacag ctctattatt
     7141 aatcaattgt cgaatatatc tcctattaaa tgggttaatg atagtttgtt ctatcttatt
     7201 tttggtggtc aatataatcc gattcctgta actttgatcg ttaatattag tatcggaaca
     7261 atttttataa tattggcatt gataggtatg agaaagcagg tgacgacatg atttgtttta
     7321 taaaaactct atttgttaag ataaagcgca aaaaaacaag ttatgtgaca ttctttttaa
     7381 tgcctatttt aacgacactt ttggctttat ctttgagttt ttcaaataat aatcaggcta
     7441 aaattgggat attagataaa gataatagtc agatatctaa acaatttatt gctcaactaa
     7501 agcaaaacaa aaagtacgac atttttacca aaatcaaaaa agagcatatt gatcactacc
     7561 ttcaagataa atctctagaa gcggtattga ctattgacaa aggattctca gataaagtgt
     7621 tgcaaggaaa atctcaaaaa ctcaatatac gttcgattgc taatagcgaa attacagagt
     7681 gggtaaaggc acagaccaat tatctattgg aaaactataa tattataggg gatgtggctc
     7741 taggtaacga agatactttt aatagaattc tccaaaaaaa ccaacagtta aattatgatg
     7801 ttaagcaagt aactttgaca gaccgatcac gtagcaaagc agtatcttcc acaacgacag
     7861 gatttttgct tattctaatg ttaggtagta cgagcgtgat ttacagtggt attttggcag
     7921 ataaatctgg tcagttatac catcgattaa tgttatcgaa cttatctcgt tttcgctata
     7981 tgttgagtta cgtctgtgta ggatttgttg cttttacgat tcaaattgtc attatgctga
     8041 gtttgttaaa agtatttaat attagttttt ttgttccgac atcacttctt ttgattattt
     8101 ttttcctttt tagtttatta gcgataggct ttggcctgct gattggagct atcacacaaa
     8161 attcccaaca aagtagccag ttagctaatc ttattgtaat gccgacttca atgttggctg
     8221 gctgtttatg gccattgtct attacgcctt cttatatgca agcaattggt aagttattac
     8281 cacaaaattg ggttttatca gccatagcta tttttcaaag tggagggacc ttatctcagg
     8341 cttggccata cttgttagct ctaatgggaa cagcactagc tttgattagt ttttcaagtt
     8401 tattattaaa acctaccaaa ctataattag tcttttgtta aaaaatactt tcctaattag
     8461 ctaactacca ggtagccctt ttattggctt atatgcaata aaagggttgc tttttgctta
     8521 tattttctct agtgcactta tcttttgtta aaagcgattg cgatgttact gttgttactg
     8581 tgttttttag agagcatatt atcaaaagcg acctctctag aaagttcgtt tttgttgaca
     8641 gattagttgt caaagtattt taaaaaaacg ttttcaataa acataaaaac taatgttatt
     8701 aaaaaaagtt gtgaaaacgt atttatttta tggaatgttt ttgttacaat aagcatgtat
     8761 cgaatgctat aaagctgtct tgttcgctat cagggatgct tgtgatgtta tgtcagatca
     8821 aacaaatctt taattgggtg acttaacaga ttaaaagaaa agatctaatc gtgttacttg
     8881 atagtataat taatgcaaac agaaagtgta tcccaattca tagtctcagg tggaggttaa
     8941 tattcaaaaa taaaatcttg ttgttgtctt aaactaatca cattaaaagg agatatcatg
     9001 attaacaaga aatatataat acctgtttca ttgttgacac tagctattac gcttactagt
     9061 gttgaagaag ttacttcacg ccaaaatttg acttatgcca atgaaatcgt aacacaaagg
     9121 ccaaagagag aatctgttat tagtgataaa tcgaattttc ccgtcatatc accttaccta
     9181 gcaagtgtgg attttggtga gagaaaaaca tctttgccaa cacctgataa aggagtaaaa
     9241 gtaactactg aacagtctat tgctcaagta agaaaggggc ctgaagaaag aacctatact
     9301 gttactggca agattacgag tgtgatcaat ggctggggag gctatggctt ttatattcaa
     9361 gatagtgaag gtattggact ttatgtttat cctcaaaaag atttaggata cagtaaggga
     9421 gatattgttc aattaacagg tacacttact cgctttaaag gtgatttaca actccaacag
     9481 gtgactgcac acaaaaagtt agagttatct tttccgactt ctgttaaaga agcagtaata
     9541 tcagaattag aaacaacaac accctcaaca ttagttaagt tatctcacgt gacagttgga
     9601 gaattatcaa ctgatcaata taacaacaca tctttccttg taagggatga tagtggtaaa
     9661 agtatagttg ttcatataga tcatcgtaca ggggttaaag gggctgatgt tgttactaaa
     9721 ataagtcagg gtgatttgat taacctcaca gccatattgt ctattgttga tggtcagtta
     9781 caattaagac cgttttctct tgaacaattg gaagtggtta aaaaggtcac aagctcaaat
     9841 agtgatgctt catctcgtaa tattgtgaaa ataggcgaga ttcaaggagc tagtcatacg
     9901 tcgccacttc tcaaaaaagc ggtcaccgta gaacaggttg ttgtcactta tttagacgat
     9961 tccactcatt tttatgttca agatcttaat ggtgatggtg atttagcgac ttcagatggt
    10021 attcgtgttt ttgctaaaaa cgctaaggtt caagtcggcg atgttttgac catttcaggt
    10081 gaagtggaag aattctttgg tcgtggttat gaggaacgta agcagactga ccttaccatc
    10141 acccaaattg tggctaaagc agtgatcaaa acagggacag ctcaagttcc atcaccgctt
    10201 gttttaggga aagatcgtat cgcgccagcc aatattattg ataatgatgg cttgcgtgtg
    10261 tttgatccag aagaagacgc tattgattat tgggaatcaa tggaaggcat gttagtggcg
    10321 gttgatgatg ctaaaatcct tggtccaatg aaaaataaag aaatttatgt cttacctggc
    10381 tctagtacaa gaccgttaaa taattcaggt ggagtattac ttccagctaa ttcttataac
    10441 acagatgtga ttcctgttct tttcaaaaaa ggcaaacaaa ttattaaagc aggagactct
    10501 tacaaaggaa gattagctgg gccagtatct tatagctatg gtaattacaa ggtctttgtt
    10561 gatgacagca aaaacatgcc atgtttaatg gatggtcatc taaaacctga aaaaacaaac
    10621 ttgcaaaaag accttagcaa gttaagcatt gcttcttaca atattgaaaa cttctcagcc
    10681 aatccttctt caactaaaga tgagaaggtc aaacggattg ccgaatcctt tattcatgat
    10741 ctgaatgctc cagacattat tggattaatt gaagtccaag ataataatgg gccgactgat
    10801 gatgggacaa cggatgcgac acaaagcgcg caacgcctca ttgatgctat taaaaaacta
    10861 ggtggcccaa cttatcgtta tgttgatatt gctccagaaa ataatgttga cggaggtcaa
    10921 ccaggtggta atattcgaac aggattcctt tatcaaccag agcgcgtcag cctttctgat
    10981 aagccaaaag gcggtgctcg tgatgctcta acttgggtta atggagaatt aaaccttagt
    11041 gttggtcgaa ttgatccaac taacgccgct tggaaagatg ttcgtaaatc gctagcagca
    11101 gaatttatct tccaaggtcg taaagtcgtt gttgttgcaa atcatttgaa ctctaagcgt
    11161 ggggataatg ctctttatgg tcgtgtgcaa ccagtcactt ttaaatctga gcaaagacgt
    11221 cacgtcttgg ctaatatgct agcacaattt gcgaaagaag gggcaaaaca ccaagctaat
    11281 attgtgatgc taggtgactt taatgatttt gaattcacaa agacgattca attaatcgaa
    11341 gaaggtgata tggttaactt ggtgaaccga catgatattt cagatcggta ttcttatttt
    11401 caccaaggca ataatcagac ccttgataat atattagttt cacgccattt acttgatcac
    11461 tacgaatttg acatggttca tgtgaattcc ccatttatgg aagctcacgg acgcgcatca
    11521 gatcatgatc cattgttact tcaattatca ttttctaaag aaaatgataa ggcagagtct
    11581 tctaaacaaa gtgtaaaagc taaaaaaact tcaaaaggaa aactgttgcc aaaaacagga
    11641 gatagtcttg tttatgtgat aacgctacta ggaacggcta gtttattagt gcctatttta
    11701 ttattgacta aaggcaaaaa ggaatcatag cgatttgaag aattggagtt cataaagagt
    11761 cgttaaaggc ttgatatact tacgttagac cttaagctaa aatggatgac tatataaaaa
    11821 ctaatattga taacaaaaga agataacaat aagacagctt tacttgctaa agacttttgt
    11881 caagctaatt gttttatggt acaatgtaac cgttgctttg caacccttgg tgcttagttc
    11941 cctttcacca agcatattag aaacgtagat agcttttgag agttatccag acgttaaagg
    12001 agaaaaaatg acaaaattga ctgtacacga ctatgttcac attgggctag tagctgcctt
    12061 gtatgttgtc ttaactatta ccccgccttt aaatgcgatt tcttatggga tgtatcaatt
    12121 tagaatatct gaaatgatga atttcttagc tttttatcat cgcaagtata ttattgcggt
    12181 gactcttgga tgtatgattg ctaactttta tagtttcgga ctgattgatg tctttgtagg
    12241 cggcggttca actcttattt ttgtgacctt aggtgttatt ttgtttagta agtatcaaaa
    12301 agattatctt tttaatggca tttttaataa agcttttgtt tacttttctt tcttttttgc
    12361 aacttccatg tttactgtag caatcgaact ttacttcttt ggagctccat ttttattaac
    12421 atggtttaca actgcgttgg gagaattagt atctctgctc ataggatcac ttattattga
    12481 taagttgtct caaagaattt ccttttagta atgcatcata ctcaaaagag ttgggtcaat
    12541 acccttctct tttttatgtt gtttgtaatg ttaaaatcgt tctagataga aatatttaac
    12601 actttagcta atagtaccat atggagtggc caacgattac ttttgatgct gttatgatat
    12661 agaaatagct catttgttat cttttatgat aaaataaaag tatgaaaaaa cgcataaaag
    12721 aattaacaga tcttcttaac cgttaccgtt atgactatta tactaaagat gccccatcag
    12781 tttctgacag tgactatgat aagctttatc gtgaactggt gactttagag cagtcatatc
    12841 ccgagtatgt cttgcaagat agcccaacgc agcaggttgg agggactatt ttaaaggggt
    12901 ttgaaaaata tcgacatcag tatccgcttt ttagtttgca ggatgctttt tcacgtgagg
    12961 agctggatgc ttttgataag cgcgtcaagg ccgaatttcc gaatgccacc tatctagcag
    13021 agttaaaaat tgatggttta tctatttcct tgagttatga gaatggcttc ttacaggttg
    13081 gagcaacacg gggtgatggc aatatcggtg aaaatatcac agaaaatatt aaaaaaataa
    13141 aggatattcc tcaccaattg agtgagcctt taaccatcac cgttcgagga gaggcttaca
    13201 tgtctcgtca gtcctttaaa gcgatcaatg aagcacgcca agaaaatggg gagactgaat
    13261 ttgctaatcc tagaaatgct gccgctggga cactaaggca gctagatacg gcagtggttg
    13321 ctaagcgtca actagccact tttttgtatc aagaagctag tccgacagct agaaatcaac
    13381 aaaatgaggt cttagcagaa ttagcagatc taggtttttc agtaaatccg tattatcaac
    13441 tcacatcgtc tatggatgag atttggaatt ttatcaaaac tattgaggca aaaagagatc
    13501 aattagctta tgacatagat ggcgttgtta ttaaagttaa tagcctagcc atgcaagaag
    13561 agctagggtt tacggttaaa gctccacgct gggctattgc ttacaaattt ccagcagaag
    13621 aaaaagaggc agaaatcctt tcggttgact ggacagtggg acgaacaggc gtggtcacac
    13681 ctacagcgaa tctaactcct gtgcaactag cggggacaac cgtgagtcgt gcaaccctac
    13741 ataatgtgga ttatattgcc gaaaaagata tccgtattgg cgatacagtg attgtatata
    13801 aggcagggga cattattcct gctgtcttaa atgtcgttat gagtaagcgg aaccagcaag
    13861 aagtcatgct tattcctaaa ctatgcccat cttgtggtag tgaattagtt cattttgaag
    13921 atgaggttgc tttgcgttgt attaatccgt tgtgtcctag tttgattcag cgaagtttgg
    13981 agcattttgc aagtcgtgat gccatgaaca ttactgggct gggaccagct attgtggaaa
    14041 aactattttt agcggggttt gttcatgatg ttgctgatat ttaccagttg accaaagaag
    14101 atttcatgca attagatggc atcaaagaaa agtcagctga taaattgctc gctgcgattg
    14161 aggcttctaa atcaaattca gctgaaaaac tgttatttgg tctaggaatt cgtcatattg
    14221 gctctaaggt tagtcgctta attttagagg tatacggaga tatttctgct cttttaaccg
    14281 ctaaagaaga ggaaatcgca aggatagatg gtcttggctc aacgattgct caatcactta
    14341 ctcaatactt tgaacaaaaa acggctgcga ttttagtaga cgagttaaag acagcaggtg
    14401 tcaacatgca ttactcaggt caaaaagtta atagtgacgc agcgcttttt ggtctaactg
    14461 ttgttttaac tggcaaatta aatcagctga accgaaatga ggccaaagat aaattagagg
    14521 cattaggtgc caaagttact ggaagtgtat ctaagaaaac agatttagtt attgcaggta
    14581 gtgacgctgg ttctaagcta gaaaaagcaa agagcttagg aattcggatt gaagatgaag
    14641 attggctacg ccagctttaa gtgaggtaat gttatgaaaa aacaattgag agcacgtctc
    14701 atttataatc caacgtcagg acaagaactt atgagaaaaa gtgttcctga agttttagac
    14761 atattagaag ggttcgggta tgaaacatct gcctttcaaa caacagccca aaagaactct
    14821 gctctaaatg aagcaagaag agcggcaaag gcaggttttg acttattgat tgctgctgga
    14881 ggtgatggca ctattaacga agtggtcaat ggaattgctc ccttgaaaaa gcgccctaaa
    14941 atggccatta ttccaacagg taccactaat gattttgcta gagcgttaaa agtaccaaga
    15001 ggaaatccga gccaagcagc aaaacttatc ggaaaaaatc agactatcca gatggatatt
    15061 ggtagagcta agaaagatac ttatttcatc aatattgctg cagcaggaag tttgacggaa
    15121 ttaacttaca gtgtaccaag tcagttaaaa acgatgtttg gatacttggc ttatcttgct
    15181 aaaggagtag aactgctccc aagagttagc aatgtgcctg ttaaaattac gcatgacaaa
    15241 ggcgtctttg aaggtcaagt ttctatgatt tttgcagcta ttaccaattc tgttggggga
    15301 tttgaaatga ttgctcctga tgcaaaacta gatgacggta tgtttacgct cattttaata
    15361 aagacagcaa atctttttga aattgtacat cttttgcgtt tgattttgga tggtggtaag
    15421 catattacag atcgtcgtgt cgaatacatt aagaccagta aaatagtcat tgaaccccaa
    15481 tgtggtaagc gaatgatgat taatttagat ggcgagtacg gaggagatgc tccaattaca
    15541 ttagagaacc taaaaaatca tatcactttt tttgcagata ctgatttgat ttctgatgac
    15601 gctttagtct tagatcagga cgaattggaa attgaggaaa tcgtaaagaa atttgctcat
    15661 gaagtagaag acttagaaca agaattagaa gaataataca atagttatta gctactgaca
    15721 gtttagtcaa atgcactact attagaatct aagattttta aattaattga ggtagtgttt
    15781 tttgatgtgc tcaaagagtc aatttattca tatgaggggc ccatttgagg tctctctttt
    15841 ttgatcatat gcaagaaaat gccatgtttt agggatgtta ttataccctt tttggaatct
    15901 tgttgccttt ttgttataat aagagtgtaa ttctattttt aaaaagatct taacaaagac
    15961 agactcgttt atcaaaaagg ttagtattag tctaagaaag cgtactcagt ctaaagaaat
    16021 ctgttgacat tcttaatttt aaggaataga atagcaaagt aaaaaaaatt taaaggagta
    16081 ttctactatg aatcctattt tcgcattggc tttggcctgt tttggtgttt ctcttgccga
    16141 aggtttcttg atggcaaatc tttttaaagc agcatctcgc caaccagaaa tcattggtca
    16201 attacgttca ttaatgatct tgggtgttgc ctttatcgaa ggtactttct ttgttaccct
    16261 tgttatggcc ttcattctta aataacagcc tatttgataa tagaaaagga gggtgttaga
    16321 tggaagaagc taaaataccc atgctaaagc ttggtccaat aacctttaat ttgaccctac
    16381 ttgctgtttg tattgtcaca attgctgtta tctttgcctt tgttttttgg gcaagtcgcc
    16441 aaatgaaatt gaaaccagaa gggaagcaaa ctgctttaga atatttaatc agttttgtgg
    16501 atggtattgg agaagagcac ttagatcata atctacaaaa atcttattcg ctgttactct
    16561 ttaccatttt tctctttgtg gctgtcgcta ataatttagg gttatttact aagttagaga
    16621 cagttaatgg ctataaccta tggacctcgc caacagccaa tctggctttt gaccttgctc
    16681 tatctctttt tattacctta atggtacaca ttgaaggggt tagacgtcgt ggcttggttg
    16741 cccatttgaa acgtttggct acaccgtggc caatgactcc gatgaattta ttagaagagt
    16801 tcacaaattt cttatcactt gccattaggt tattcggtaa tatctttgcc ggggaagttg
    16861 ttacaggttt gattgttcaa ctggccaatt atcgagttta ttggtggccg atcgctttcc
    16921 tagtcaatat ggcgtggaca gccttttcag tctttatttc ctgcatacag gcttttgttt
    16981 tcacaaaact gacagcgacc tatctaggaa agaaagttaa tgaatcagaa gaataaggaa
    17041 ggagaacaaa cgaatgtcaa tcacatttgg tgaactcgtt gggaatttta ttctagtaac
    17101 aggttctgta atcgtcttgt tactcttgat caaaaaattc gcttggggtg caattgaatc
    17161 gattttacag acacgctcac aacaaatctc tcgagatatt gatcaggctg agcaatcacg
    17221 tttaagtgct caacagttag aggcaaaaag tcaagctaac ctagatgcta gtcgttcaca
    17281 agcaagtaaa atcattagtg atgccaaaga aattggtcaa ttacaaggtg ataaattggt
    17341 ggcagaagct actgatgaag caaaacgctt gaaagaaaaa gcgttgacag atattgaaca
    17401 aagcaaatca gacgctattt cagcagtcaa aacagaaatg tctgatttaa cggttctttt
    17461 agcggaaaaa attatgggag ccaatcttga taagacggcg caaagccagc ttattgacag
    17521 ttatcttgat gacttaggag aagcttaatg accaaaaaag aacaggctct tatcgagcaa
    17581 tatgcaaaaa gccttgttga ggtagcaagt gaacatcatt ctcttgatgc cttacaagct
    17641 gatgtgcttg cgattttaga gacgtttgta acaactaatc ttgatcaaag tctatcaagt
    17701 ctagctgttc cgcatgctga aaaaataaaa ttgctaacct tattaaaggg aaataattct
    17761 gtttatatga ataatttctt gaatctcatt ttacaaaacg aacgtgaagc ttatttgtat
    17821 caaatgcttc agactgtttt aaatgaaatt gcaatagttt caaatcagta tgatgtgact
    17881 gttacctcat cactaccttt gactgaggag caaaaaagcc gtgttcgagc agttgttgca
    17941 aagaaatttg ctgtaacagc gggtcgttta attgaaaaag tagatccttc gcttattgga
    18001 ggatttatta tcagcgtcaa taataaagta attgatacca gtattcgtcg tcaattgcaa
    18061 gcatttaaaa tgaatttaaa atagaaagtg gtgtgacttt tggcaattaa tgcacaagaa
    18121 attagcgctt taattaaaaa gcaaattgag aacttccagc caaatttcga tgtcacagaa
    18181 actgggatcg ttacctatat tggtgacggt attgcacgtg cccgtggatt agataatgcc
    18241 atgagtggtg aacttcttga atttgaaaat ggtgcctatg ggatggctca aaaccttgag
    18301 tcaaatgatg taggtattat tattcttggt gatttttcag caatccgtga aggagatgtg
    18361 gttaagcgca ccggtaaaat catggaagta ccagttggtg aagctctcat tggacgtgtg
    18421 gtaaacccac ttggacaacc agttgatggc ttgggagata ttgaaacaac tggctttaga
    18481 ccagtcgaaa cgccagctcc tggtgttatg cagcgtaaat ccgtttctga acccttacaa
    18541 actggattaa aagcaattga tgctttggtt ccaattggtc gtgggcaacg tgagttgatt
    18601 atcggagacc gtcaaacagg aaaaacatcc gtagctattg atgcgatttt gaaccaaaaa
    18661 ggtcaagaca tgatttgtat ctatgtggct attggtcaaa aagaatcaac cgttcgtaca
    18721 caagttgaaa cccttcgccg ttacggtgcc ttggattata ctattgttgt gacagcgtca
    18781 gcctcacaac catcaccatt gctcttcatc gctccttatg cgggtgttgc catggctgaa
    18841 gaatttatgt accaaggcaa gcatgtcttg attgtctatg atgatttatc aaaacaagcg
    18901 gtggcttatc gtgaattgtc tctcttgctt cgtcgtccac caggtcgtga agcttaccca
    18961 ggggatgttt tctacttgca tagccgtctt ttggaacgct ctgcaaaagt ttctgatgac
    19021 ttaggtggag gttctatcac agctctacca tttatcgaaa cacaggccgg agatatctct
    19081 gcatatattg cgactaacgt tatttcgatt acagacggac aaatcttctt gcaagagaac
    19141 ctctttaatt caggaattcg tccagcgatt gatgcaggat catctgtctc acgtgtaggt
    19201 ggttcagctc aaattaaagc gatgaaaaag gttgctggta ctcttcgttt ggatttggct
    19261 tcctatcgtg aattagaagc ctttacccaa tttggttcgg accttgatgc tgcaacacaa
    19321 gcaaaactta atcgtggtcg ccgtacggtt gaaatcttga agcaaccatt gcataaaccg
    19381 ttacctgttg aaaagcaagt cgttattctt tatgccctta ctcatggttt cttagatgat
    19441 gttcctgtag atgatatttt ggcttttgaa gaagctcttt acgactactt tgacgttcat
    19501 tacaatgatt tatttgaaac tatccgtacc acaaaagacc ttcctgaaga agctgccctt
    19561 gatgccgcta ttaaagcgtt taaagaacac tcaaacttta agtcatagag ggaggatact
    19621 agtatggcag gctctctaag tgaaattaaa gctaaaatta tttcaactga gaaaacaagt
    19681 aaaatcacta gtgccatgcg tatggtttct tctgcaaaac ttgttaaatc tgagcaagca
    19741 gctcgtgatt ttcaaattta cgcctcaaaa attcgccaga ttactacaga tttattgaaa
    19801 tcagaattaa ccataggatc tgataatccg atgttggtgt ctcgtcctgt caaaaaaaca
    19861 ggctatattg tcatcacgtc tgataaagga cttgttggag gttacaattc aaaaatcttg
    19921 aaatctgtta tggatatgat tacagagtac catgctgatg gtgactatga aatcatttct
    19981 attggtagtg ttggttcaga cttcttcaaa gcaagaggga tgaatgtggc ttttgaactt
    20041 cgtggacttg cggatcaacc aagttttgaa caagtgagac aaatcatttc acaatccgtt
    20101 gacatgtttg tcaatgagat ttttgatgag ttatatgtct gttacaatca ccatgtgaat
    20161 agcttaacca gccaagtccg tgttcaacag atgttgccaa tttctgactt ggttgctgat
    20221 gaagcagcag aagaaggtgt tactgggttt gaattggaac ctaatcgtca tgatatttta
    20281 gatcaattgt tacctcaatt cacagaaagt ttaatttatg gggctatcat tgatgctaaa
    20341 acggctgaac atgcagcagg tatgactgcc atgcagacag cgacagataa cgctaagaat
    20401 gtcattaacg atttgaccat ccaatataac cgagctcgtc aagcagcaat tacacaagaa
    20461 attactgaaa ttgttgcagg agcaaatgca ctagaataaa aaataacgct gactagcgat
    20521 taggttattg tcagcattca attttatcaa tcgagatgaa atgacttatg ggacttttct
    20581 attgaaagac tgctcttttc agtataaaag aagttagcaa gactaactaa gccctgacta
    20641 tcgaatataa ggagaaatga atgagctcag gcaaaattgc tcaggttgtt ggtccagtag
    20701 tagacgttat gtttgcgagt ggggacaaac ttccagagat taataatgca ttgattgttt
    20761 ataaagatag tgataaaaag caaaaaatcg tccttgaagt tgctctggaa cttggtgacg
    20821 gtatggtgcg aacaatcgct atggaatcaa ctgatgggct tacacgtggg ttagaagttc
    20881 ttgacactgg tcgtgcgatt agcgtaccag taggtaaaga aactttggga cgcgtcttta
    20941 atgtacttgg agaaaccatt gatttggaag aaccatttgc agaagatgtt gaccgtcagc
    21001 cgatccataa aaaagcacca tcgtttgatg aattatcaac atcatcagaa attcttgaaa
    21061 ctggtatcaa ggtaattgac cttcttgccc cttaccttaa aggtggtaaa gttggacttt
    21121 tcggtggtgc cggagttggt aagactgtcc ttatccaaga attaatccac aatatcgccc
    21181 aagaacacgg aggtatttca gtatttaccg gtgttggtga gcgaacacgt gaaggaaatg
    21241 acctttactg ggaaatgaaa gaatcaggcg ttattgaaaa aaccgccatg gtttttggtc
    21301 agatgaatga gccgcctggg gcacgtatgc gtgtagccct tactggttta accattgctg
    21361 agtatttccg tgatgtagaa ggccaagatg ttttgctctt tattgataat atcttccgtt
    21421 tcactcaggc aggttcagaa gtatcagccc tcttaggccg tatgccttct gctgttggtt
    21481 accaaccaac acttgctact gaaatggggc aattgcaaga acggattaca tcaactcaaa
    21541 aaggttctgt tacatctatc caagcgattt atgtaccagc agatgattat actgaccctg
    21601 cgccagcaac agcatttgct cacctggatt caacaactaa ccttgaacgt aaattgacac
    21661 aaatggggat ttatccagcc gttgatccat tggcttcaag ttcacgggct ttatcacctg
    21721 agattgttgg tgaagagcac tatgctgtgg caacagaagt tcaacgtgtg cttcaacgct
    21781 accgtgaatt acaagacatc atcgctattt tggggatgga tgaattatca gatgaagaaa
    21841 aaacattggt tggacgtgcg cgtcgtatcc agttcttcct ctcacaaaat tttaacgttg
    21901 ctgaacaatt taccggtctt cctggttctt acgtgccagt tgctgagaca gttcgcggct
    21961 ttaaagaaat tcttgaagga aaatacgatg atcttccaga agacgccttt cgttcagttg
    22021 gtccaatcga ggatgtgatt aaaaaagccg aaaaaatggg cttttagtga ggtgatgcca
    22081 tgacacaaat gactgttcaa gtggtaacgc cagatggaat caaatatgat caccatgcta
    22141 aatgtatttc ggtaacaaca ccagatgggg aaatggggat tttacctaat cacatcaatt
    22201 taattgcacc tttgcaagtc catgaaatga aaattcgccg aggtggtgaa gatgaaaaag
    22261 tggactggat tgctatcaat ggtggtatta ttgaaatcaa agataatgtg gttaccgttg
    22321 tcgctgattc tgcagaacgt gaccgtgata tcgatgtcag ccgagcagag cgagcaaaac
    22381 taagagcaga acgtgaaatt gcacaggctg aaacgactca caacattgat gaagttcgtc
    22441 gtgccaaggt tgccttgcgc cgcgctttga atcgtatcaa cgttagtaaa aagtaagtgg
    22501 tgttgataaa agacaaggat aagtaagaaa ggtttttaac ctattatttt gtttgctgag
    22561 tctttttaac atcagtttct gagagtttga ccatactacc ttctctaaac acgttattat
    22621 tgttgagagg agttatatgg gggaactctt ttttgtatcc acgttgttag tagaaactca
    22681 gtaatgacgc ttaatgtatt tttaggctat caacttagtc agagaagatg tcaacttaat
    22741 tgataagttg gacggatcaa gatggtatgt tataataatc ctatggaatt attaatgaag
    22801 atcattgcct tttcgtgtca tttatttttt attggcctta gttaccaatt actgattagt
    22861 gtgattgact ggacaaaatt tagtcattat catccagaaa atcttggccg gctacgactt
    22921 tttgtctttt tattagctac tgccctaggg tatttggtga gccattgtat gctggaattg
    22981 cttcaaatta gccagacgct cttttttgag ttccgctaat actggaataa aagaagtatg
    23041 gtataatttt actacgacta agagatagat aagggagaaa attgtggata aaataatcat
    23101 tgaaggtggt caaacacgct tagaaggaga agttgttatt gagggtgcta aaaacgcagt
    23161 tcttccttta ctggcagcat ctattttacc ttcaaaagga aaaacaattc ttagaaatgt
    23221 tcctattcta tctgatgttt ttaccatgaa taacgtggta cgtggtttag atattcgtgt
    23281 tgattttaat gaagcagcta acgaaatcac agttgatgcc tcaggacaca ttttagatga
    23341 agccccttac gagtacgtga gccaaatgcg tgcctctatt gttgttttag gaccaatttt
    23401 ggcacgtaat ggccacgcaa aagtatcaat gcctggaggc tgtacgattg gcagtaggcc
    23461 tattaatttg cacttaaaag gattagaggc tatgggggca acgattactc aaaaaggtgg
    23521 agatattacc gctcaagcag atcgactgca aggtgccatg atttatatgg acttcccatc
    23581 tgtaggagct acccaaaact tgatgatggc tgccacttta gctgatggtg tgaccactat
    23641 tgaaaatgct gctcgtgagc ctgaaattgt tgatttagct caatttttga ataaaatggg
    23701 agctagaatt cgtggtgctg gaacagagac gttaacgata acaggcgtca caagtcttca
    23761 tggggtggag cacgatgtgg ttcaagaccg tattgaagca ggtactttta tggttgcagc
    23821 ggcgatgact tcaggcaatg ttttgattag agatgctgtt tgggaacata atcgtccatt
    23881 gatttcaaaa ttaatggaaa tgggtgtttc tgtaacagaa gaagaatatg gtattcgcgt
    23941 tcaagcaaac acgccaaagc tcaaacctgt aacggttaaa accttgcctc atccaggttt
    24001 tccaacagac atgcaagctc aatttacagc tttaatggca gtggtcaatg gagagtctac
    24061 tatggttgaa actgtctttg aaaatcgatt tcaacatctg gaagaaatgc gacgcatggg
    24121 cttacaatct gaaatcctta gggaaactgc catgattcat ggtggaagac aactgcaagg
    24181 tgctcctgtg atgtcaacag atttacgtgc cagtgctgcc cttatcttga cagggattgt
    24241 tgctcaagga gtgacaatag ttaataattt ggttcattta gatcgtggct actatcaatt
    24301 tcatgaaaaa ttagcaaagc ttggagctac tattagtcgt agcagtgagg tctagcttat
    24361 gacaaatggt ttaaaatatg tcttagaaca aatgttatta cttttagtaa ttgctgcctt
    24421 ggcgtgtctt tttttagcca ttggacttat gattggttat agttttatgg gagatggcca
    24481 tagtccatgg catattttat ctatggataa gtgggcagag ttagtgaata aatttactgg
    24541 aaagtaggcc ttgcctactt tttatagcat ttgagaggaa gtatgttaac aaggaaaaaa
    24601 cgaaaaagga tgcttaattc cttcttattg attttgttag tgttggggtc taacagcctt
    24661 gctaagagtg atttcatagc tagcactcac cctatcaagc aactttatca gactcttgtt
    24721 ggggagaaga aaaaatcatt actcacacat aataatttgc ccgctgatgt tcccacagaa
    24781 gctctggcgg catcggtatt gacaccatca atcaaaaagc aactaggtcc agatgttcag
    24841 tggaatggag caggcgccta tattatcaac aggaacaaaa cagatttaac tgctaaggta
    24901 gccagtccgc cttatgccaa caatgaaacc aaatgggtgc aagagcaaaa ggttcctact
    24961 agagctaatg ccttacttac taagtcgaca cgacagtatc aaaatcgcta tcaaacgaac
    25021 aatgcttatc gtacctttaa gccagcaggt tggcaccgat tgcaccactt aaaaggaagt
    25081 tatgatcacg cggttgatag agggcatttg ctaggttatg ctttagttgg tggactgaaa
    25141 ggttttgatg cttaccagta atcctgataa tattgccact caactaagtt gggctaatca
    25201 agtaaataaa ccctactcga caggtcagaa ctactatgaa ggtttagtac gtcgtgcttt
    25261 ggataagggg catcgcgtcc gctaccgtgt caccctactt tatgatggtg ataatctact
    25321 agccagcggt agccatttgg aggctaaatc ctctgatgac agcttgactt ttaacgtgtt
    25381 tgttccaaat gtccaaccag gattgaccgt tgactatcgg acgggacaaa tagctatcaa
    25441 tctctaagcg aaaagtcact gatttaagat tgtcactaag cacttttttt gatagaatag
    25501 aactaattga ataagtgtct atgagactag tactaataga gaaattaaca gggagtgagg
    25561 gccgtgactg gaagccttct taagggaact attaggaatt cactcagcta aagacaatta
    25621 attaaggtct gataacatca gataaaaacg gatggtaccg cgtgtcaacg ctccgcttat
    25681 gaagtggagt agtggcacgc ttttttcatc gctaaaagaa gggaaaacta tggatttaca
    25741 agcacaatta gaggaattaa aaacaaaaac attggagact ctccaatctt tgacggggaa
    25801 tcataccaaa gaactgcaag atttgcgcgt ggctgttctt gggaaaaagg gatctttgac
    25861 agaattacta aaggggctaa aagacttgtc taatgacttg cgtcctgtgg ttggtaaaca
    25921 ggtgaacgaa gttcgtgact tattgacaaa agcctttgaa gaacaggcta aaattgtaga
    25981 agctgctaaa attcaagccc agcttgatgc tgaaagcatt gatgtgactt taccgggtcg
    26041 tcaaatgact ctgggccacc gtcatgtgtt aacacaaacc agtgaagaaa tcgaagatat
    26101 tttcttagga atgggttttc agattgttga tggctttgag gtagaaaaag attattacaa
    26161 ctttgagcgc atgaatttgc caaaggatca ccctgcccgt gacatgcaag atacgtttta
    26221 tatcacagaa gaaatcttgt tacgaactca caccagtcct gtgcaggccc gtactcttga
    26281 tcaacatgat ttttcaaaag ggcctttaaa aatggtttct ccaggtcgtg ttttccgtcg
    26341 tgatactgat gacgctaccc actcacatca attccatcaa attgaaggtt tggttgtggg
    26401 caaaaatatt tccatgggag acctcaaagg gactcttgaa atgattatca agaagatgtt
    26461 cggtgaagaa cgtagcattc gtttgcgccc gtcatatttc ccgttcacag aaccatctgt
    26521 tgaagtggat gtttcttgct ttaaatgtgg tgggaaaggc tgtaatgtct gcaagaaaac
    26581 aggatggatt gaaattcttg gtgctggtat ggttcaccca agtgtcttgg aaatgtcagg
    26641 tgttgatgca aaagagtatt caggctttgc ctttggttta ggtcaagagc ggattgccat
    26701 gttgcgttat ggcattaatg acattcgtgg tttctatcaa ggcgaccaac gtttctctga
    26761 acaatttaac taaaatgagt cgtttgaaga cttctgtcag ctattttatc taagattgag
    26821 tgaaagttga taggtaatga taataggtta attgtctaag gggaaagtcc ctctcgcttc
    26881 aacgtcataa gggttttgac ttggaactta aaatacgaaa ggaaatgact caaatcacgg
    26941 cttgagagct ttctctagcc gagtgatttg ggcactaaaa ttatgttagt atcttataaa
    27001 tggttaaaag aattagtaga tatcgatgtg acaccagcag cacttgctga gaaaatgtcg
    27061 gcaacgagta tcgaggtaga aggaattgaa gtccctgcag atggtttatc aaaactggtt
    27121 gtggggcatg tgctttcttg cgaagatgtg ccagagacac atttgcattt gtgtcaggta
    27181 gatacaggtg atgaaacgcc acgtcaaatt gtgtgtggcg caccaaatgt gaaagcaggg
    27241 attaaggtta tcgtggctgt gccaggtgct cgtattgcgg ataactataa aatcaaaaaa
    27301 ggcaaaatcc gcggcatgga atctcttggc atgatttgtt ccttgcaaga acttggtttg
    27361 tcagatagta ttattccaaa agaattttca gatggtattc aaatcttgcc agaggaggct
    27421 gtgccaggtg atgctatttt taaatacctg gacttggacg atcatatcat tgaattgtcc
    27481 attacaccta accgtgctga tgcgctttct atgcgaggtg tggcacatga agtcgcagct
    27541 atttatggca agtcagtatc attcccccaa aaaaatctac aagaatctga taaggcaact
    27601 tcagaagcca ttgaagtggc cattgcctct gataacgttc taacttacgc tagccgtgtc
    27661 gttgaaaatg tcaaagttaa gccaagtcca caatggttgc aaaacctttt aatgaatgct
    27721 ggtattcgtc ctattaataa tgtggttgat gtgaccaact atgtgctctt gtattttggt
    27781 caacctatgc atgcttttga ttatgataaa tttgaagacc ataagattgt ggcgcgtgcg
    27841 gctcgtcaag gggaaagttt agtcacactt gatggtgaaa aacgggattt gaccactgag
    27901 gatcttgtta ttaccgtggc tgacaaacca gttgctttgg caggtgttat gggtggtcaa
    27961 gccactgaaa ttgatgccaa ctctcagacg gttgttcttg aagttgctgt ttttgatggc
    28021 aaatcgattc gtaaaaccag tggtcgttta aaccttcgtt cggaatcctc ttcacgtttt
    28081 gaaaaagggg ttaactatgc aactgtttta gaagcgcttg actttgcagc ggctatgtta
    28141 caagaattag cagaaggtca agtcttatca ggccatgttc aagctggaca attgccaaca
    28201 gaacctgttg aagtctcaac tagccttgac tatgtgaatg ttcgtttagg aacagaatta
    28261 acttttgctg atattcaaag gatttttgac caacttggat ttggtctaac aggagatgaa
    28321 acaagcttca cggtagctgt tcctcggcgt cgttgggatg ttagtattcc ggctgatttg
    28381 gtggaagaaa ttgcgcgtat ttatggttat gataagttgc caacaacctt accagaggca
    28441 ggaggaacgg ctgcagaatt aacgccaaca caggctcttc gtcgtaaggt ccgtggttta
    28501 gcggaaggat tgggcttgac tgaaatcatt tcgtatgcct tgacaacacc agagaaagct
    28561 gttgagtttg cagtggcacc aagtcaccta acagaactca tgtggccaat gtctgtggaa
    28621 cgttcagcgc ttcgtcaaaa tatggtttca gggatgttag atacggtagc ttataatgtg
    28681 gctcgtaaac aaagtaactt ggctctttat gaaattggaa aaatctttga acaagaagct
    28741 aatccaaaag aagaccttcc taatgaattg aaccattttg cttttgctat ttgtggctta
    28801 gtggcacaaa aagatttcca aacccaagct caggcagtgg atttttacca tgctaaaggt
    28861 atcttggata ctttatttgc aaaccttaac ctcaaggttc aatatgtgcc aaccaaagac
    28921 ctagcaagca tgcacccagg acggacagcg cttatcctat tagatgagca agtgattgga
    28981 tttgttggtc aagttcatcc aggaacagca aaggcttata gtattccaga aacctatgtg
    29041 gcagaattgg atatggctgc tttagaagca gcgttgccaa gtgatcaaac ttttgcggaa
    29101 atcacaaaat tccctgctat gacacgtgat gttgccttgt tacttgaccg tgaagtgagt
    29161 catcaggcca ttgtaacagc tattgaatca gcaggtgtaa aacgcttgac taagattaag
    29221 ctctttgacg tgtatgaagg agtaactatt caggctggca aaaaatcaat ggcttatagc
    29281 cttactttcc aaaatccaaa tgacaattta acggatgaag aagttgccaa atacatggag
    29341 aaaatcacca aagcattgac agaacaagtc ggtgctgaag tacgctaaga aaaaaactgg
    29401 gcctctaagg ctccgttttt ttctgttata atagaggaaa aaagaactcc agttcaaatg
    29461 attggagaaa aacatcagaa ggtggaggaa aatcagaatg tatcaaacaa aaataacagg
    29521 caaccgcctt taccacacgg tttcgaaagg ctacggagat agtgtaacct tatttggaaa
    29581 aactgatcaa ggggaaaccc ctatgagttt actggttatt gccttgtctt cttgtgtgac
    29641 tatgtgtgtg cagggttttt accatcgtca atttcagcaa gacacacttg ctgtttctgt
    29701 tgaggctagt tatgaggaag aagtttttga actacttgtt aggattgatg atgttttaga
    29761 tgacaaaaag aaagctgatt tgttggctta cattggaact tattgtcgtg tgaaacaact
    29821 cctcaaacct gaagttatgg tgaccattac gttggaagga ctgtctcatg tctgatgctg
    29881 gtttgaaaac ttaccaaaaa caatgggctt atcaaaagta ttgggtcatg gcacactcac
    29941 agcagcatta caatgcttta agagaattgt ttaaaggaaa tcagtggtca gaagaaaagg
    30001 tgttgacttt tcattgtttg attgaagaag cgcaagccat tcctcccaca gtaaaaagct
    30061 tacgaacagc atatcagcat gtttgggggt attttaaaaa ggtagcgagt caggaagaaa
    30121 aagaccattt caaagatttg gatgctcagt tggaaactaa atctgaggag atgctatgct
    30181 ttttacaaga gatgacggct cattatcagc cctcttatct gctatcttgc cgtttgatta
    30241 ccaaaggtcc ttaaatgcta aacagaaaag tagggacttc tgggaagtta tgctaagcta
    30301 gtgatataaa caagggggaa tgagagatgt ttttagcgct taatgaaatg aaacaatcca
    30361 aattgcggta tggtttgatt gctggtttgc tatgtttggt tgcctatttg atgttctttt
    30421 tgtcaggatt ggcctttggt ttgatgcagg agaaccgctc ggcggttgat ctttggaaag
    30481 ctgacagtat tttactagcc aaggatgctg acgctacctt gaccttatca caggtgtcca
    30541 gagctcaaga aaatcaaata acagcagaca aggtagctcc tcttgctcaa ctcaataccg
    30601 tggcgtggtc agttaaaaat cctaaggatg ccgacaaagt taaggttagc cttttcggga
    30661 ttgattctaa tagctttatt cgtcctaaca ttgtaaaagg tcgattattt aagactaaca
    30721 aagaggttgt tttggatcaa agccttgcaa aagaggaagc ttttgcgatt ggcaaggact
    30781 tttacacatc gagttcgagt caagcattaa ctatcgttgg ttatactcaa aatgctagat
    30841 ttagtgttgc accagtggtt tatatgaatc tggaagcttt tgaaacatta aaatatggag
    30901 aaccactacc aaaagataag caagttgtta atgcttttat cactaaagga agtttaacag
    30961 attatcctaa aaaagacttc caaaaattag atattaaaac ctttattact aaattacctg
    31021 gttatagcgc tcaactttta acgtttggct ttatgattag ttttcttgtc attatttcag
    31081 ctattattat tggtattttt atgtatattt tgactattca aaaggcacct atttttggga
    31141 ttatgaaagc gcaaggaatt tctaacaaaa cgattacgac cgctgtgctt atgcagacat
    31201 tctttttgag ttttttaggt agtggtttag ggttgctagg tacttggctg acatcattat
    31261 tattaccaac agtagttcct tttcaaagca attggttttt atatttggct atattcgtta
    31321 gtatgatctg ttttgctcta ttaggcacct tattttctgt ttttaacatt atacgaattg
    31381 atcctttgaa agcaattgga taggaggtag tgatgtcggt tttaacattt aaacaagtga
    31441 ccaaaacatt tcaagatggt catcacgaaa tcaacgcatt aaaggcaact gacttttcca
    31501 ttgaagctgg agaatttgtg gctattattg gaccatcagg ttctggaaaa tcaacctttt
    31561 tgaccattgc tggtggctta cagaccccaa gttctggtca gctaatcatt gatggcactg
    31621 attatacgca tttatcagaa aaagagcgct cacgcttgcg cttcaagtcc gttggtttta
    31681 tcttacaagc ttccaatctg ataccttttt taacagtcca gcagcagttg gaattggtcg
    31741 atcatttgac aggaagcaaa gaaaaagcca aagccaatca gttatttgac gatttaggca
    31801 ttacagggct aaaacatcag ttacctcaag aattgtccgg cggagaaagg caaagagcgg
    31861 cgattgcccg tgctctctac catgatccag cactgatatt ggcagacgaa cctactgcaa
    31921 gtttggatac tgaaaaagcc tatgaagtgg tcaaactgtt agctaaggaa agtaaagaaa
    31981 aaaacaaggc cattattatg gtcactcatg atgatcggat gctaaaatat tgtgacaaag
    32041 tctatcggat gcaagatgga gagttacgtc aagagagata gtagactggt aagagtttat
    32101 gatattagaa gaaaaaaacc aaacaagcac agcacttgtt tggttttttt ctaagtgtga
    32161 ctattatcaa tacgtcacca tttccctaaa gcttactata cgggatgcta aaggtatcac
    32221 catgctcaaa gtcgccatat tggtaacctc ggtcaaacca acgttggcgt tgcttagaag
    32281 tgccatgtgt aaaactatca ggaacggttc tgccataggt ttcttcttgc agagtatcat
    32341 ccccaactgc gtgagctgct gccattgctt cctcaatatc tcctttttcc aaaaggcctt
    32401 gcccttgaac ataattagcc caggcaccag catagtaatc agcttgtaat tccaacttga
    32461 cgttgagctg attggcttta gcctttgatt ttccttgtct agcgctagcg taattatcca
    32521 taattcccaa ttcattttgg atgtggtggc caacttcatg agcaatcaca taggccatag
    32581 caaaatcacc ttttgcgccg tacttggttg atagttcatt ataaaaagaa atatctaaat
    32641 acactttttg atcgcctggg caataaaaag gaccagaaga ggcttgaccc ctgccacaag
    32701 cggtttgagt tgctccagta tatagaacta atgttggttt atgataagtt aaccctttct
    32761 cacgaaatgt cttagtccaa tagtcctcgg tagaggcgaa gaccttactg acaaaagaaa
    32821 cctgttcttg actagccttg tcaccatttg tacgcgttac ttggcttgat tgataggcat
    32881 tgttgttggt agaagaaggc ttgcctccta aaacactaga taggccaccc cctcccataa
    32941 ctaggagaag gagaagaata accagcttgg ttttccaacc tcctcgtgaa aagagtaatt
    33001 gaagcaggag accgccccct aggccgccgc ccccaaaaga accagacgat tgaccacgtc
    33061 tatcttcaac ctgttgactt tctctcaaat catccgtttt cataagtttc tcccatctat
    33121 ttcttgattt tattataaaa taattctatt agaattgcta tttttatgat gagattataa
    33181 ctaaaaatag gaaaagcaat aggtatttgc tataatataa caagggtaga ttcagtttgt
    33241 ataaaggagt catatgaaat taatttacac agagatgagt tactcgatga cagagatttt
    33301 ggtgaatgaa gcacgaaaag ctgctgacca aggttatcga gtgttctata ttgctccaaa
    33361 ttctctttca tttgaaaagg aaagagaagt cttaacctta ttgcctgaac gagggacttt
    33421 ttcgataata gtaacccggt tcgttcaaat gtcaaggtat tttacagttg agagctctcc
    33481 ttctaagcaa catctagatg acacgacttt ggctatgatt ttttatcggg ccttgatgca
    33541 attaaaacca gaagatttgc cgagttatgg tcgtttgcaa aataattcgg tttttattga
    33601 acaactagtt gagttatata aggaattaaa aaacgcacaa ttaagcgtac atgatttaac
    33661 aggattagac catcctcaaa aacaagaaga tcttattaaa attattgagc ttgcagagac
    33721 gatcatgatt cagcaagatt ataatcaaga tagtcctcta caatcttttg caagagcaat
    33781 taagttagga cttttaaata accaactatc aaaaacagtt attgttattg atggtttttc
    33841 acgtttttca gctgaagagg attatttatt atcactttta aataacaact gtcaagaggt
    33901 catcattggc agttatgtga gtcaaaaagc ttatcaaaaa tcttttatta agggaaatat
    33961 ctatgaggct agccttcatt ttttgcaaga tttggctcaa aaataccata tcaagccggt
    34021 atttgcgacc tctaatcaag tgtttaaacc agccttttca cggctaacac aattatttga
    34081 agcaactcat gatttttcac aagtagactg gcaactccaa aagaatgatc ttgatcactt
    34141 tagcctttgg caatgtcatc atcaaaaaga ggaaattgaa catgtagcaa aatccattcg
    34201 gcaaaagcta tatgaaggtt accgttataa ggatatctta gtgttgctag gagatatgga
    34261 tgcttatcaa ttacaaattg ggcctatctt tgataagttt gaaatcccct actatttagg
    34321 aaaagcagag cctatggctg cccatccatt agtccaattt atagaatcgc tcgaacgaag
    34381 tcagcgctat aattggcgtc gtgaagacat tttaaacatg ttaaaatcag gactttttgg
    34441 ttgttttgat gatagtgata ttgatcgctt tgaggaatat actcagtttg cagatattaa
    34501 aggatttact aaattttcaa agccttttac cattaactct tctaggcagt atcctcttga
    34561 ctttttaaat gaaatgagac aagacatcgt tttacctttg caagaattgt ttaagagtca
    34621 aaaacaactt ggtgcttcac ttgttgacaa attaatactt ttttttaaaa agattagatt
    34681 agctgagaat atgcaaggtt tagcacagtc ccaattagag gtggaaaaaa acgaagaagt
    34741 ttggaaaagg tttacagata ttttgacttc ttttcatcat atctttggcc aagaaaaatt
    34801 acggctatca gattgccttg ctttaatcaa aacgggaatg aaatcagctc agtatcgtgt
    34861 tgttccagcg acattagatg ttgtcactat taaatcttat gaccttgttc aaccacattc
    34921 aaaacctttc gtttatgcaa ttggtctaac tcaatcacat tttcctaagc aaattcacca
    34981 ttcaggacta ctttctgatc aagagcgtgc tagaattaat gaaataagaa attatcgtca
    35041 ttttgatatt gctagtgccg aaaatagtaa aaaaaaccat caaactgcgt tatctctgtt
    35101 taatgctgct actaaagagt tagttcttag tgttccaacg gtaatcaacg agacattcga
    35161 tgatctatct ccttatctta aagaattaat aagctttggg ctacctcttt tggataaagg
    35221 caaaaactat ctatcttatg ataatagcga tattggtaac tataaagctc tcttgtcaca
    35281 aattattgct attaaccgac aagatcttat agaaatgtca gatcaagata agatgttttg
    35341 gactgttgtc ttacgttatc tcaggaaaca gttaagaaag caacagctag agctacctac
    35401 ttcagattat cgtttatcaa caaagccttt gtctaaagaa gttatcgagg tatgttttcc
    35461 aaagggaatc cctttaaagc tatctgcaac tgctttaacc gttttttata ataatcagta
    35521 caactatttt ttgaaatatg ttttgaattt aaacaaaaca gaatccattc atccagattc
    35581 cagaattcat ggtcaatact tgcatcgtgt ctttgagcgg ttaatgaaag atcacacgca
    35641 agaaccgttt gataataaat taaaacaagc aatctaccat accaatcaag aaagtttttt
    35701 tcagcaggtt taccaagata atgccgaggc tgagtacagt ttagctatac tagaagatat
    35761 tgttagatca acagcaccta tcttgcaact taaccaaaac ataaaggtca ttgatcaaga
    35821 aaagaacttt catctggata tgggaaatga gatattagtc cacgggatca ttgatcgtat
    35881 tgaccagctg agcgacggta gcttgggcat tgttgactat aaatcaagcg cgaatcaatt
    35941 tgatattggg accttttata atgggttgag tccacaacta gtgacttacc tcgcagcctt
    36001 aaaacagatc gcaccgcacg atataaacca attatttggg gctatgtacc ttcatttgca
    36061 agatcctaaa ttggatctag taacatttaa acaaattgat aacacattag tggagagtat
    36121 ctataaagcg ttgacctata aaggtatttt ttcagaagtc gaaaaagagc atctatctac
    36181 tggtgcttac caaaccaaaa atgctcttta ttccaatgac gaattggaaa cacttttaaa
    36241 ttataacaag tacctctatc ttaaagctgc taaacacatt aaaaagggac attttttgat
    36301 taatccttat acaagcgatg gtaagacagt tcaaggagat caattaaaag ccattacccg
    36361 ttttgaagca gatcttgaca tggcacaggc aaggcgacta gtgactctcc ctgctaagga
    36421 gaaaaaagag tgctttttaa cattaatgag aaaggagagc cacttgtgat ttcttttgcc
    36481 ccatttttaa gccccgaagc tattaaacat ttacaagaaa acgaaaggtg cagtgatcag
    36541 tctcaaaaac gcacagctca acaaattgaa gcaatttata ctagtggcca aaatatactt
    36601 gtatcagctt ctgctggttc aggaaaaacc tttgtaatgg tcgaacgcat acttgataaa
    36661 attttgagag gtgtttcaat tgatcggctt tttatctcaa cctttactgt taaagcagct
    36721 acagaactgc gtgaacggat tgaaaacaaa ttatactcac aaattgctca aactacagat
    36781 tttcaaatga aagtttattt aacagaacaa ttgcaatctc ttggtcaagc tgatattggt
    36841 actatggatg cttttgcaca gaaagtagta agtcgctatg gttatagcat tggcatttca
    36901 tcccaatttc gtatcatgca ggataaagca gaacaagatg ttttaaagca agaggtgttt
    36961 agcaaactct ttagtgagtt tatgaatcaa aaagaggcac cggtgtttag ggctcttgtg
    37021 aaaaattttt ctggtaactg taaagacact tcagctttta gagagttagt ttatacttgt
    37081 tattctttta gccaatcgac agaaaatcca aaaatatggt tgcaagaaaa ttttctaagc
    37141 gctgctaaaa cttaccaaag gcttgaagat atcccggatc atgatattga actcttactt
    37201 ttggcaatgc aagacactgc aaatcagcta agagatgtga ctgatatgga agattatggg
    37261 cagctgacta aggcaggtag ccgatctgct aaatacacta aacacttaac gatcatagaa
    37321 aagttgtctg attgggtgcg tgattttaaa tgtttgtatg gaaaagccgg attggatcgg
    37381 ttgatcagag atgtgacagg ccttatacca tctgggaatg atgttacagt ctcgaaggta
    37441 aaataccctg tttttaagac cttgcatcaa aaattaaaac aatttaggca tttagaaaca
    37501 attttaatgt atcagaaaga ctgttttccc ttattggaac agttacaaga ttttgtgctt
    37561 gcgttttcag aagcttattt agctgtcaaa atacaagaaa gtgcttttga attttcagat
    37621 attacacact ttgcaatcaa aattttagag gaaaatacgg atattcgcca atcctatcag
    37681 caacactatc atgaggtgat ggttgatgaa tatcaagata acaatcatat gcaagagcga
    37741 ctcctgacct tactatcgaa cggtcataat cgctttatgg taggagatat caaacaatcg
    37801 atctatcgat ttcggcaagc cgatcctcag atttttaatc aaaagtttag agactatcaa
    37861 aaaaaacctg agcaggggaa agtgatttta ctcaaagaaa actttcgtag ccaatcagag
    37921 gtgttaaatg tcagcaatgc tgtttttagt cacttaatgg acgaatcagt aggagacgtc
    37981 ttatacgatg agcaacatca gttaatagca ggtagtcatg ctcaaacagt cccctatcta
    38041 gaccgtcgtg ctcagttatt gctatataat agcgataaag atgatggcaa cgccccttca
    38101 gatagtgagg gtatttcatt tagtgaggtt acaattgttg ccaaagaaat tattaagctt
    38161 cacaatgata agggtgtccc ttttgaagac attacgttac tcgtttcttc aagaacaaga
    38221 aatgatatca tttctcatac attcaatcaa tatagtattc ctatagtaac agatggtggg
    38281 cagcaaaact atcttaaatc tgttgaagtg atggttatgt tagatacatt acgcaccatt
    38341 aataacccaa gaaatgatta tgcccttgtg gctttactgc gctcaccgat gtttgccttt
    38401 gatgaggatg atttagcaag aatagcactt caaaaagaca atgagctaga taaagattgc
    38461 ctatatgaca agatacaaag ggctgtgatt ggaagaggtg ctcatcctga attgattcac
    38521 gataccttgc ttggcaagtt aaatattttt ttaaagacgt tgaaaagttg gcgtcgatac
    38581 gctaagctag ggtcgttgta tgacttgatt tggaaaattt ttaatgatcg tttttatttt
    38641 gattttgtag ctagtcaagc aaaagcagaa caagcacaag ctaatctata cgcattagct
    38701 ctacgtgcta atcagtttga aaaatcgggc tataaagggc tataccgttt tattaaaatg
    38761 attgataagg tacttgagac gcaaaatgac ttagcagatg tggaagtggc tgctcctaaa
    38821 caagctgtta atttaatgac cattcacaag tctaaaggtt tacaatttcc gtatgtattt
    38881 atccttaatt gtgacaagcg cttctcaatg acagatattc ataaatcatt tattctgaat
    38941 cggcagcacg gtatcggtat caagtacctt gcagatatca aaggtttact tggtgaaaca
    39001 acactcaatt ctgttaaagt aagcatggaa accttacctt atcaattgaa caaacaagag
    39061 ttgcgcttag caactttatc agaacaaatg cgcttattgt atgttgctat gacacgagct
    39121 gaaaaaaaag tttattttat tggtaaagct agtaagagca aaagtcaaga tatcacagat
    39181 cctaaaaagt taggcaaact tttgccgctg gctttacgag aacagttatt gacattccaa
    39241 gattggctat tagcaatagc agatatattt tcaactgaag atctttattt tgatgttcgc
    39301 tttattgaag atagtgattt gacacaagag tcagtcggac gacttcaaac accacagtta
    39361 ttaaatccag atgatcttaa agataatcgt caatcagaaa caattgcacg ggctttagat
    39421 atgttagaag cagtgtctca attgaatgcc aattatgaag cagctattca tttgccaaca
    39481 gttcgaacgc ctagccaact taaggtagct tacgagcctt tattagaacc cattggtgta
    39541 gatattatag agaaatcttc tcgatcgcta tctgatttta ctttgccaca tttttcaaaa
    39601 aaagtaaaag ttgaagcaag tcatattgga tcagctcttc atcagttgat gcaggtgctc
    39661 cctttgtcaa aaccgataaa tcaacaaacg cttttagacg ctttaagaga aattgatagt
    39721 aacgaagagg taaaaacagc tcttgatctc aaaaaaatag agtcgttctt ttgtgataca
    39781 agcctaggcc aattttttca gacttaccaa aaacacttgt atcgagaagc accatttgct
    39841 attttaaaag ttgaccctat tagtcaagaa gaatatgtcc tacgtggtat tatagatgcc
    39901 tactttttgt ttgatgatca tattgtatta gtggactata aaacagataa atacaagcag
    39961 cccattgagt taaaaaagcg ttaccaacaa cagttggagt tatatgcaga agctctcact
    40021 caaacgtata aacttcctgt gactaagcgc tatcttgttt taatgggagg tggaaagcca
    40081 gaaattgtcg aagtttaagg ttgttgtgac acaagggtat tttgcaaatg ataatagtat
    40141 agttgaggat tttacgaaat gcttgaaaag ctattgacct ttgaa
//
LOCUS       DATXIG010000012        39952 bp    DNA     linear   BCT 28-DEC-2023
DEFINITION  TPA_asm: Streptococcus pyogenes strain MGAS11113
            SAMN08555799-rid19682583.denovo.012, whole genome shotgun sequence.
ACCESSION   DATXIG010000012 DATXIG010000000
VERSION     DATXIG010000012.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN08555799
            Sequence Read Archive: SRR6740495
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pyogenes
  ORGANISM  Streptococcus pyogenes
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 39952)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 39952)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-OCT-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-OCT-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001918058.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001918058.2
            Genome Coverage       :: 368x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/12/2023 11:23:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 1,806
            CDSs (total)                      :: 1,769
            Genes (coding)                    :: 1,722
            CDSs (with protein)               :: 1,722
            Genes (RNA)                       :: 37
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1, 1 (5S, 23S)
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 47
            CDSs (without protein)            :: 47
            Pseudo Genes (ambiguous residues) :: 0 of 47
            Pseudo Genes (frameshifted)       :: 30 of 47
            Pseudo Genes (incomplete)         :: 20 of 47
            Pseudo Genes (internal stop)      :: 9 of 47
            Pseudo Genes (multiple problems)  :: 11 of 47
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..39952
                     /organism="Streptococcus pyogenes"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN08555799-rid19682583.denovo.012"
                     /strain="MGAS11113"
                     /serotype="Emm28"
                     /isolation_source="normally sterile site"
                     /host="Homo sapiens"
                     /db_xref="taxon:1314"
                     /country="Finland"
                     /collection_date="2002"
                     /collected_by="National Public Health Laboratory"
     gene            176..1024
                     /locus_tag="VC122_000623"
     CDS             176..1024
                     /locus_tag="VC122_000623"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011055028.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PucR family transcriptional regulator"
                     /protein_id="HEP5640567.1"
                     /translation="MELKDYFPEMQVGPHPLGDKEWVSVKEGDQYVHFPKSCLSEKER
                     LLLEVGLGQCEVLQPLGSPWQRYLLDHQGNPPQLFETSQFIYLNHQQALPDDLVELLQ
                     QMIAGLEVILPISATQTAFLCRQAISIKVLRWLEDLLPTLESDFGLALTMFVGNAWYQ
                     VAAGTLRECFEEECQLLTAYLRQQSGRKLLTFSGLMLWSLLSHHTFLALTRQFHQSLS
                     PQSDMADVVHALWSEHGNLVQTAQRLYIHRNSLQYKLDKFAQQSGLHLKQLDDLAFAH
                     LFLLKY"
     gene            1322..2455
                     /gene="ugpC"
                     /locus_tag="VC122_000624"
     CDS             1322..2455
                     /gene="ugpC"
                     /locus_tag="VC122_000624"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002988014.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sn-glycerol-3-phosphate ABC transporter
                     ATP-binding protein UgpC"
                     /protein_id="HEP5640568.1"
                     /translation="MVELNLNHIYKKYPNTTHYAVEDFDLDIKDKEFIVFVGPSGCGK
                     STTLRMIAGLEDISEGELKIGGEVVNDKSPKDRDIAMVFQNYALYPHMTVYDNMAFGL
                     KLRKYKKDDIDRRVKEAAQILGLTEFLERKPADLSGGQRQRVAMGRAIVRDAKVFLMD
                     EPLSNLDAKLRVSMRAEIAKIHRRIGSTTIYVTHDQTEAMTLADRIVIMSATKNPQGN
                     GTIGKIEQVGSPQELYNLPANKFVAGFIGSPAMNFFEVEVKDGRIVSEDGLAIAIPEG
                     QAKMLEAAGYKGKKVTFGIRPEDISSRQIVHDTYPSATVTAEVLVSELLGSETMLYVK
                     LGQTEFASRVDARDFHSPGEQVSLTFNVAKGHFFDRDTEQAIR"
     gene            2584..4197
                     /locus_tag="VC122_000625"
     CDS             2584..4197
                     /locus_tag="VC122_000625"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002993683.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-glucosidase"
                     /protein_id="HEP5640569.1"
                     /translation="MKKHWWHKATIYQIYPRSFKDTSGNGIGDLKGITSQLDYLQKLG
                     ITAIWLSPVYQSPMDDNGYDISDYEAIADVFGDMADMDELLAAANERGIKIIMDLVVN
                     HTSDEHAWFVEARENPNSPERDFYIWRDEPNDLTSIFSGSAWEYDNASGQYYLHLFSK
                     RQPDLNWENEALRHKIYDMMNFWIAKGIGGFRMDVIDLIGKVPDSEITGNGPRLHDYL
                     KEMNQATFGNHDVMTVGETWGATPEIARQYSRPENKELSMVFQFEHVGLQHKPDAPKW
                     DYAEELDVPALKTIFSKWQTELKLGEGWNSLFWNNHDLPRVLSIWGNDSTYREKSAKA
                     LAILLHLMRGTPYIYQGEEIGMTNYPFKDLTEVNDIESLNYAKEAMGNGVSAARVMDS
                     IRKVGRDNARTPMQWSKDTHAGFSEAKETWLPVNPNYQDINVADALADPDSIFYTYQK
                     LIALRKEQDWLVEADYHLLPTADKVFAYQRQLGEETYVIVVNVSDEEQVFAKDLTGAQ
                     VIITNTDVDTVFETKHLQPWDAFCLKLKA"
     gene            4395..7892
                     /locus_tag="VC122_000626"
     CDS             4395..7892
                     /locus_tag="VC122_000626"
                     /EC_number="3.2.1.41"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011889168.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pullulanase"
                     /protein_id="HEP5640570.1"
                     /translation="MKKKVNQGSKRYQYLLKKWGIGFVIAATGTVVLGCTPSILTHQV
                     AAKTIVGLARDEAQQGDGNAKSGDGLQSSSKEAKPVLDSSLANPASIAEHHLRMHFKT
                     LPAGESLGSLGLWVWGDVDQPSKDWPNGAITMTKAKKDDYGYYLDVPLAAKHRQQVSY
                     LINNKAGENLSKDQHISLLTPKMNEVWIDENYHAHAYRPLKKGYLRINYHNQSGHYDN
                     LAVWTFKDVKTPTTDWPNGLDLSHKGPYGAYVDVPLKEGANEIGFLILDKSKTGDAIK
                     VQPKDYLFKELDNHTQIFVKDTDPKVYNNPYYIDQVSLKGAEQTTPNEIKAIFTTLDG
                     LDEDAVKQNIKITDKAGKTVAIDELTLDRDKSVMTLKGDFKAQGAVYTVTFGEVSQVA
                     RQSWQLKDKLYAYDGELGATLAKDGSVDLALWSPSADTVKVVVYDKQDQTRVVGQADL
                     TKSDKGVWRAHLTSDSIKGISDYTGYYYLYEITRGQEKVMVLDPYAKSLAAWNDATAT
                     DDIKTAKAAFIDPSKLGPTGLDFAKINNFKKREDAIIYEAHVRDFTSDKALEGKLTHP
                     FGTFSAFVEQLDYLKDLGVTHVQLLPVLSYFYANELDKSRSTAYTSSDNNYNWGYDPQ
                     HYFALSGMYSVNPNDPALRIAELKNLVNEIHKRGMGVIFDVVYNHTARTYLFEDLEPN
                     YYHFMNADGTARESFGGGRLGTTHAMSRRILVDSIAYLTREFKVDGFRFDMMGDHDAA
                     AIEQAFKAAKAINPNTIMIGEGWRTYQGDEGKKEIAADQDWMKATNTVGVFSDDIRNT
                     LKSGFPNEGTAAFITGGAKNLEGLFKTIKAQPSNFEADAPGDVVQYIAAHDNLTLHDV
                     IAKSINKDPKVAEEEIHKRIRLGNTMILTAQGTAFIHSGQEYGRTKQLLNPDYRTKVS
                     DDKVPNKATLIDAVAKYPYFIHDSYDSSDAVNHFDWAKATDSIAHPISNQTKAYTQGL
                     IALRRSTDAFTKATKAEVDRDVTLITQAGQDGIQQEDLIMGYQTVASNGDRYAVFVNA
                     DNKTRKVVLPQAYRYLLGAQVLADAEQAGVTAIAKPKGVQFTKEGLTIDGLTALVLKV
                     SSKTANPSQQKSQTDNHQTKTPDGSKDLDKSLMTRPKRAKTNQKLPKTGEASSKGLLA
                     AGVALLLLAISLLMKRQKD"
     gene            8007..8381
                     /locus_tag="VC122_000627"
     CDS             8007..8381
                     /locus_tag="VC122_000627"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011106906.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiol reductase thioredoxin"
                     /protein_id="HEP5640571.1"
                     /translation="MRPKEKETSMTFEEIVANFIPSSVAEVTSAIASGKDMIVFLGRS
                     SCPYCRRFAPKLAQVATDNQKEVYFVDSENAADAAELAAFRENYQLVTVPALLVSYDQ
                     HQRAVCDSSLTPDDILAFLTRE"
     gene            8497..8862
                     /gene="yajC"
                     /locus_tag="VC122_000628"
     CDS             8497..8862
                     /gene="yajC"
                     /locus_tag="VC122_000628"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010922684.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit YajC"
                     /protein_id="HEP5640572.1"
                     /translation="MGFPTILMFVVMLGLIWFMQRQQKKQAQERQNQLNAIEKGDEVV
                     TIGGMFAIVDEVDTTAKKIVLDVDGVFLTFELSAIKRIVTKATTETTLVETEETSVVN
                     EPAVETVDSTDTDSAIESH"
     gene            9081..9830
                     /locus_tag="VC122_000629"
     CDS             9081..9830
                     /locus_tag="VC122_000629"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015057262.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="isoprenyl transferase"
                     /protein_id="HEP5640573.1"
                     /translation="MFGLKAKSTKKVLGSIPKHIGIIMDGNGRWAKKRLKPRVFGHKA
                     GMDALQEVTITASELGVKVLTVYAFSTENWSRPQDEVSFIMNLPVTFFDKYVPVLHEN
                     NVKIQMIGETSRLPEDTLAALNAAIDKTKRNTGLILNFALNYGGRAEITSAVRFIAQD
                     VLDAKLNPGDITEDLIANYLMTDHLPYLYRDPDLIIRTSGELRLSNFLPWQSAYSEFY
                     FTPVLWPDFKKAELLKAIADYNRRQRRFGKV"
     gene            9843..10637
                     /locus_tag="VC122_000630"
     CDS             9843..10637
                     /locus_tag="VC122_000630"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002275264.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatidate cytidylyltransferase"
                     /protein_id="HEP5640574.1"
                     /translation="MKERVVWGGVAVAIFLPFLIIGNLPFQLFVGVLAMIGVSELLKM
                     KRLEVFSFEGVFAMLAAFVLAVPMDHYLTFLPIDANVAFYSLMVFFILAGTVLNSRAY
                     SFDDAAFPIATSFYVGIGFQHLINARLSGIDKVFLALFIVWATDIGAYLIGRQFGRRK
                     LLPTVSPNKTIEGSLGGIACAVLVSFIFMVIDRSVYAPHHFLTMLVLVALFSIFAQFG
                     DLVESALKRHFGVKDSGKLIPGHGGILDRFDSMIFVFPIMHLFGLF"
     gene            10710..11969
                     /gene="rseP"
                     /locus_tag="VC122_000631"
     CDS             10710..11969
                     /gene="rseP"
                     /locus_tag="VC122_000631"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011055022.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RIP metalloprotease RseP"
                     /protein_id="HEP5640575.1"
                     /translation="MLGIITFIIIFGILVIVHEFGHFYFAKKSGILVREFAIGMGPKI
                     FSHVDQGGTLYTLRMLPLGGYVRMAGWGDDKTEIKTGTPASLTLNEQGFVKRINLSQS
                     KLDPTSLPMHVTGYDLEDQLSITGLVLDETKTYKVAHDATIVEEDGTEIRIAPLDVQY
                     QNASIGGRLITNFAGPMNNFILGIVVFILLVFLQGGMSDFSSNHVRVQENGAAAKAGL
                     RDNDQIVAINGYKVTSWNDLTEAVDLATRDLGPSQTIKVTYKSHQRLKTVAVKPQKHA
                     KTYTIGVKASLKTGFKDKLLGGLELAWSGAFTILNALKGLITGFSLNKLGGPVAMYDM
                     SNQAAQNGLESVLSLMAMLSINLGIFNLIPIPALDGGKILMNIIEAIRRKPIKQETEA
                     YITLAGVAIMVVLMIAVTWNDIMRVFF"
     gene            12167..14023
                     /locus_tag="VC122_000632"
     CDS             12167..14023
                     /locus_tag="VC122_000632"
                     /EC_number="6.1.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015898574.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="proline--tRNA ligase"
                     /protein_id="HEP5640576.1"
                     /translation="MKQSKLLIPTLREMPSDAQVISHALMVRAGYVRQVSAGIYAYLP
                     LANRTIEKFKTIMREEFEKIGAVEMLAPALLTADLWRESGRYETYGEDLYKLKNRDNS
                     DFILGPTHEETFTTLVRDAVKSYKQLPLNLYQIQSKYRDEKRPRNGLLRTREFIMKDG
                     YSFHHNYEDLDVTYEDYRQAYEAIFTRAGLDFKGIIGDGGAMGGKDSQEFMAVTPART
                     DLDRWVVLDKSIASMDDIPKEVLEDIKAELAAWMISGEDTIAYSTESSYAANLEMATN
                     EYKPSSKVAAEDALAEVETPHCKTIDEVAAFLSVDETQTIKTLLFVADNEPVVALLVG
                     NDHINTVKLKNYLAADFLEPASEEEARAFFGAGFGSLGPVNLAQGSRIVADRKVQNLT
                     NAVAGANKDGFHVTGVNPGRDFQAEYVDIREVKEGEMSPDGHGVLQFARGIEVGHIFK
                     LGTRYSDSMGATILDENGRTVPIVMGCYGIGVSRILSAVIEQHARLFVNKTPKGDYRY
                     AWGINFPKELAPFDVHLITVNVKDQVAQDLTAKLEADLMAKGYDVLTDDRNERVGSKF
                     SDSDLIGLPIRVTVGKKAAEGIVEIKIKATGDSIEVNAENLIETLEILTKEH"
     gene            14277..18674
                     /locus_tag="VC122_000633"
     CDS             14277..18674
                     /locus_tag="VC122_000633"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011889165.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PolC-type DNA polymerase III"
                     /protein_id="HEP5640577.1"
                     /translation="MSDLFAKLMDQIEMPLDMRRSSAFSSADIIEVKVHSVSRLWEFH
                     FAFAAVLPIATYRELHDRLIRTFEAADIKVTFDIQAAQVDYSDDLLQAYYQEAFEHAP
                     CNSASFKSSFSKLKVTYEDDKLIIAAPGFVNNDHFRNSHLPNLVKQFEAFGFGTLTID
                     MVSDQEMTEHLTKDFVSSRQALVKKAVQDNLEAQKSLEAMMPPVEEATPAPKFDYKER
                     AAKRQAGFEKATITPMIEIETEENRIVFEGMVFDVERKTTRTGRHIINFKMTDYTSSF
                     ALQKWAKDDEELRKFDMIAKGAWLRVQGNIETNPFTKSLTMNVQQVKEIVHHDRKDLM
                     PEGQKRVELHAHTNMSTMDALPTVESLIDTAAKWGHKAVAITDHANVQSFPHGYHRAR
                     KAGIKAIFGLEANIVEDKVPISYDPVDMDLHEATYVVFDVETTGLSAMNNDLIQIAAS
                     KMFKGNIVEQFDEFIDPGHPLSAFTTELTGITDKHLQDAKPLVTVLKAFQDFCKDSIL
                     VAHNASFDVGFMNANYERHDLPKITQPVIDTLEFARNLYPEYKRHGLGPLTKRFQVSL
                     DHHHMANYDAEATGRLLFIFLRDAREKHGIKNLLQLNTDLVAEDSYKKARIKHATIYV
                     QNQVGLKNMFKLVSLSNIKYFEGVPRIPRTVLDAHREGLLLGTACSDGEVFDAVLTKG
                     IDAAVDLAKYYDFIEIMPPAIYQPLVVRELIKDQAGIEQVIRDLIEVGKRANKPVLAT
                     GNVHYLEPEEEIYREIIVRSLGQGAMINRTIGRGEGAQPAPLPKAHFRTTNEMLDEFA
                     FLGKDLAYQVVVENTQDFADRIEEVEVVKGDLYTPYIDKAEETVAELTYQKAFEIYGN
                     PLPDIIDLRIEKELTSILGNGFAVIYLASQMLVNRSNERGYLVGSRGSVGSSFVATMI
                     GITEVNPMPPHYVCPSCQHSEFITDGSVGSGYDLPNKPCPKCGTPYQKDGQDIPFETF
                     LGFDGDKVPDIDLNFSGDDQPSAHLDVRDIFGDEYAFRAGTVGTVAEKTAYGFVKGYE
                     RDYGKFYRDAEVDRLAAGAAGVKRTTGQHPGGIVVIPNYMDVYDFTPVQYPADDVTAS
                     WQTTHFNFHDIDENVLKLDILGHDDPTMIRKLQDLSGIDPITIPADDPGVMALFSGTE
                     ILGVTPEQIGTPTGMLGIPEFGTNFVRGMVNETHPTTFAELLQLSGLSHGTDVWLGNA
                     QDLIKEGIATLKTVIGCRDDIMVYLMHAGLEPKMAFTIMERVRKGLWLKISEEERNGY
                     IDAMRENNVPDWYIESCGKIKYMFPKAHAAAYVLMALRVAYFKVHHPIMYYCAYFSIR
                     AKAFELKTMSGGLDAVKARMEDITIKRKNNEATNVENDLFTTLEIVNEMLERGFKFGK
                     LDLYKSDAIEFQIKGDTLIPPFIALEGLGENVAKQIVKARQEGEFLSKMELRKRGGAS
                     STLVEKMDEMGILGNMPEDNQLSLFDDFF"
     gene            18784..19212
                     /locus_tag="VC122_000634"
     CDS             18784..19212
                     /locus_tag="VC122_000634"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002982615.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family transcriptional regulator"
                     /protein_id="HEP5640578.1"
                     /translation="MSHLDKNTALKAMVVFRKAQRTLDAFGADIFKKADLTATQFSVL
                     EVLYTKGCMRINHLIDSLLATSGNMTVVLNNMERNGWISKCKDKTDKRAYVVTLTDKG
                     TRLIEAVLPKHVARVEEAFAVLTEKEQLCLIELLKKFKQL"
     gene            19343..19885
                     /locus_tag="VC122_000635"
     CDS             19343..19885
                     /locus_tag="VC122_000635"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011055019.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-dependent oxidoreductase"
                     /protein_id="HEP5640579.1"
                     /translation="MKHILFIVGSLREGSFNHQLAAQAQKALEHQAVVSYLNWKDVPV
                     LNQDIEANVPLPVVDARQAVQSADAIWIFTPVYNFSIPGSVKNLLDWLSRALDLSDPT
                     GPSAIGGKVVTVSSVANGGHDQVFDQFKALLPFIRTSVAGEFTKATVNPDAWGTGRLE
                     ISKETKANLLSQAEALLAAI"
     gene            complement(19918..20532)
                     /locus_tag="VC122_000636"
     CDS             complement(19918..20532)
                     /locus_tag="VC122_000636"
                     /EC_number="3.5.1.88"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009880416.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide deformylase"
                     /protein_id="HEP5640580.1"
                     /translation="MSAQDKLIKPSHLITMDDIIREGNPTLRAVAKEVSLPLCDEDIL
                     LGEKMMQFLKHSQDPVMAEKLGLRAGVGLAAPQIDVSKRIIAVLVPNLPDKEGNPPKE
                     AYSWQEVLYNPKIVSHSVQDAALSDGEGCLSVDRVVEGYVVRHARVTVDYYDKEGQQH
                     RIKLKGYNAIVVQHEIDHINGVLFYDRINAKNPFETKEELLILD"
     gene            complement(20658..20915)
                     /locus_tag="VC122_000637"
     CDS             complement(20658..20915)
                     /locus_tag="VC122_000637"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011184982.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator"
                     /protein_id="HEP5640581.1"
                     /translation="MTQNVCQQNITYTVKERFASYTLEAQANQEVHLNLTLLANRFGT
                     SDRHLKHVLKQPIFQRIIERICFKTCRIADLDALTQLSVNN"
     gene            21041..21196
                     /locus_tag="VC122_000638"
     CDS             21041..21196
                     /locus_tag="VC122_000638"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002994562.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEP5640582.1"
                     /translation="MTMLGYRLSAEVRGSAILPLLAGYLIRQAIAQVYSDLKTASWLF
                     GLASSVA"
     gene            21354..21623
                     /gene="rpsO"
                     /locus_tag="VC122_000639"
     CDS             21354..21623
                     /gene="rpsO"
                     /locus_tag="VC122_000639"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001018251.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S15"
                     /protein_id="HEP5640583.1"
                     /translation="MAISKEKKNEIIAQYARHEGDTGSVEVQVAVLTWEINHLNSHIK
                     EHKKDHATYRGLMKKIGHRRNLLAYLRRTDVNRYRELIQSLGLRR"
     gene            21976..24036
                     /locus_tag="VC122_000640"
     CDS             21976..24036
                     /locus_tag="VC122_000640"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003061769.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BglG family transcription antiterminator"
                     /protein_id="HEP5640584.1"
                     /translation="MLSHELIRNYQLFSKYKGHSLEAFESILKTSKRHILADIAKIND
                     TLSLYQLPLIALDRQLVYPPDLTEKDLLNRMLPTLDDYLFQDERLDMIIIYIMMAKEF
                     ISINHLESLLRLSRNSVIADLNLVRDRVQAFQVTLAYNRQDGYFFEGEPLALRRLLES
                     AVSSLLQVTSGPWVFSYLLHELGLPDQTKVMAATLEELSRENHLTFISEKLRDLIYFF
                     CLLAHRPFSRNVRAEVVDTFPLASPAVETMVDQLLVNFPSLTEEKYLVQSRLLGCIQG
                     DLELVFQQPIYDIMEEIINSVAVNTGLSITDTPELRQNLYSHLLPAYYRLYYDINLTN
                     PLKEQIKQDYESLFYLVKRSLSPLEKQLGKPVNEDEIAYFTIHFGRWLQAPKKRPSNQ
                     LVALSVCPNGISSSLMLEATLKELFPQLQFIRIHQLDKIKLLDPASFDLIFSTVAFDC
                     VKPVYVTQALMGPVEKMMLKKMVCDDFHLPLSEQFALDDLLSIIHKHTTITNKEGLVS
                     DLSRYLFGNHLTIEKGGLGLLDLLTADFIRQADAVSDWQEAIRLAAQPLLEHQMIETS
                     YIDGMIDSVNELGAYIVLAPKVAVPHAAPEKGTRQLGMSLLQLKEPVSFDLKQEGDPD
                     KQVQLIFVLSAVDSSSHLKALQELSLILDDDEHIEQLIEAKNTEEIMSLISHMIEKGD
                     ESHD"
     gene            24029..24313
                     /locus_tag="VC122_000641"
     CDS             24029..24313
                     /locus_tag="VC122_000641"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011018237.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HEP5640585.1"
                     /translation="MIKIVTVCGNGIGSSLLLRMKVEAIASSLGIDVDAESCDSNAAV
                     GKGADLFVTVKEFKDIFPEDAKVCIVKSYTNRKKIEEDLVPVLKEMSGKE"
     gene            24340..25719
                     /locus_tag="VC122_000642"
     CDS             24340..25719
                     /locus_tag="VC122_000642"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002982647.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS ascorbate transporter subunit IIC"
                     /protein_id="HEP5640586.1"
                     /translation="MEALLSFIRDILKEPAFLMGLIAFAGLVALKTPAHKVLTGTLGP
                     ILGYLMLAAGAGVIVTNLDPLAKLIEHGFSITGVVPNNEAVTSVAQKILGVETMSILV
                     VGLLLNLAFARFTRFKYIFLTGHHSFFMACLLSAVLGAVDFKGSLLIILGGFLLGAWS
                     AISPAIGQQYTLKVTDGDEIAMGHFGSLGYYLSAWVGSKVGKDSKDTEDLQISEKWSF
                     LRNTTISTGLIMVLFYLVATVASVLRNTSVTEELAAGQNPFIFAIKSGLTFAVGVAIV
                     YAGVRMILADLIPAFQGIANKLIPNAIPAVDCAVFFPYAPTAVIIGFASSFVGGLLGM
                     LILGVAGGVLIIPGMVPHFFCGATAGIFGNSTGGRRGAMIGAFANGLLLAFLPAMLLP
                     VLGKLGFSNTTFGDVDFGVLGILLGRLGTSIGQIGIYLVVAVLAIVLVLPNFLSKSQE
                     AINNVSEEI"
     gene            25729..26460
                     /locus_tag="VC122_000643"
     CDS             25729..26460
                     /locus_tag="VC122_000643"
                     /EC_number="2.2.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011285186.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transaldolase"
                     /protein_id="HEP5640587.1"
                     /translation="MMTKKLNVKVFSDGAVLETMLKDLQTGLVTGFTTNPSLMKKAGI
                     SSYIGFAKEVLAKITDYPVSFEVFADDLASMEKEAEKIASLGDNVYVKIPVTTSTGES
                     TCPVIQKLSAKGIKLNVTAIFTIEQTQAVVDHLTAGVPAIVSVFAGRIADTGVDPMPI
                     MEEALRICRQKEEVELLWASPRETYNIYQADQLGVDIITCTTDLIAKLPLQGKDLEDY
                     SLETVQMFLKDSTSLGFKILEDDNH"
     gene            26740..28872
                     /gene="pnp"
                     /locus_tag="VC122_000644"
     CDS             26740..28872
                     /gene="pnp"
                     /locus_tag="VC122_000644"
                     /EC_number="2.7.7.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018366801.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polyribonucleotide nucleotidyltransferase"
                     /protein_id="HEP5640588.1"
                     /translation="MSKQTFTTTFAGKPLVVEVGQVAKQANGATVVRYGESTVLTAAV
                     MSKKMATGDFFPLQVNYEEKMYAAGKFPGGFMKREGRPSTDATLTARLIDRPIRPMFA
                     EGFRNEVQVINTVLSYDENASAPMAAMFGSSLALSISDIPFNGPIAGVQVGYIDGEFI
                     INPDKEQMEASLLELTVAGSKEAINMVESGAKELSEDIMLEALLKGHQAIQELIAFQE
                     QIVAVVGKEKAEVELLQVDADLQADIVAKYNAQLQKAVQVEEKKAREAATEAVKEMVK
                     AEYEERYAEDENLATIMRDVAEILEQMEHAEVRRLITEDKIRPDGRKIDEIRPLDAVV
                     DFLPKVHGSGLFTRGQTQALSILTLAPMGETQIIDGLAPEYKKRFLHHYNFPQYSVGE
                     TGRYGAAGRREIGHGALGERALEQVLPSLEEFPYAIRLVAEVLESNGSSSQASICAGT
                     LALMAGGVPIKAPVAGIAMGLISDGTNYTVLTDIQGLEDHFGDMDFKVAGTREGITAL
                     QMDIKIAGITPQILEEALAQAKKARFEILDVIEATIAEPRPELAPTAPKIDTIKIDVD
                     KIKVVIGKGGETIDKIIAETGVKIDIDDEGNVSIYSSDQAAINRTKEIIAGLVREAKV
                     GEVYHAKVVRIEKFGAFVNLFDKTDALVHISEIAWTRTTNVSDVLEVGEDVDVKVIKI
                     DEKGRVDASMKALIPRPPKPEKKEEKHD"
     gene            28865..29617
                     /locus_tag="VC122_000645"
     CDS             28865..29617
                     /locus_tag="VC122_000645"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011285184.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SseB family protein"
                     /protein_id="HEP5640589.1"
                     /translation="MTKSNELDIRLRAFINAPDNFLDSVALVNAFHNFPVWAAKEPYV
                     IEVEGVKVTPVFTDKEDMARFKEEQESAQSQYWLERSALAVLEEVITSGAAGLVFNLK
                     KKGDFGNSTIFKSSDMIQFMNHYTTVLNTLMSDDNVAADTMEKVYLVPAFVYPKDNNH
                     YDRLFPTMSTPEGKSYVPAFSNLQSFAKWYNQDDFGGLFRKAEGVILTWTIDDIYQPR
                     NGENELDETFGVAINPFDDQQILVDWSELDKS"
     gene            29626..30207
                     /gene="cysE"
                     /locus_tag="VC122_000646"
     CDS             29626..30207
                     /gene="cysE"
                     /locus_tag="VC122_000646"
                     /EC_number="2.3.1.30"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010922671.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine O-acetyltransferase"
                     /protein_id="HEP5640590.1"
                     /translation="MGWWKESIAIVKALDPAARNSLEVILTYPGIKALAAHRLSHFLW
                     RHHFKLLARMHSQFWRFWTQIEIHPGAQIAPGVFIDHGAGLVIGETAIVEKGVMLYHG
                     VTLGGTGKDCGKRHPTVRQGALISAHAQVIGPIDIGANAKVGAAAVVLSDVPEDVTVV
                     GVPAKIVRVHGQKDNRQIQSLQKQREVSYQLSK"
     gene            30686..30916
                     /locus_tag="VC122_000647"
     CDS             30686..30916
                     /locus_tag="VC122_000647"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002982665.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEP5640591.1"
                     /translation="MNKIRVGLYVIMSLSILVSSITLVASLIFLLRQDMVKAIIYFIL
                     TLFWLIGLVINIKLLKRWNSISEKDLGYQDDN"
     gene            30944..32287
                     /locus_tag="VC122_000648"
     CDS             30944..32287
                     /locus_tag="VC122_000648"
                     /EC_number="6.1.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000591125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine--tRNA ligase"
                     /protein_id="HEP5640592.1"
                     /translation="MIKIYDTMTRSLRKFVPLTENTVNMYVCGPTVYNYIHIGNARSA
                     VAFDTIRRYFEYTGYQVNYISNFTDVDDKIIKAATQAGVSPKELSDRFIAAFIEDTKA
                     LGVKPATQNPRVMDYIAEIISFVESLIEKDFAYEADGDVYFRVEKSEHYAKLANKTLS
                     ELEVGASGRTDAETALKENPLDFALWKSAKAGEVSWDSPWGFGRPGWHIECSVMATEI
                     LGDTIDIHGGGADLEFPHHTNEIAQSEAKTGKTFANYWMHNGFVTVDNEKMSKSLGNF
                     VTVHDMLQTVDGQVLRFFLATQQYRKPINFTEKAIHDAEINLKYLKNTLQQPLTETAD
                     EQELKQFVIAFQDAMDDDFNTANGITVVFDMAKWINSGSYTEPVKSAFEKMLAVFGII
                     FEEEVLEVDIEALIAKRQEARANRDFAIADAIRDQLAAQGIKLLDTKDGVRWLRD"
     gene            32280..32693
                     /locus_tag="VC122_000649"
     CDS             32280..32693
                     /locus_tag="VC122_000649"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011889155.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Mini-ribonuclease 3"
                     /protein_id="HEP5640593.1"
                     /translation="MINPVDVNLINGIALAFEGDAVYSYYVRRHLIFQGKTKPSQLHR
                     LATRYVSAKAQANLIQAMLEAQLLTEKEEDIYKRGRNTNSHTKAKNADIITYRMSTGF
                     EAIMGYLDMMGQKERLEELIRWCIEYVEKQQLISS"
     gene            32889..33686
                     /locus_tag="VC122_000650"
     CDS             32889..33686
                     /locus_tag="VC122_000650"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012561012.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEP5640594.1"
                     /translation="MTTMQKTVSLLSLALLIGLLGTSGKAISVYAQDQHTDNVIAEST
                     ISQVSVEASIRGTEPYIDATVTTDQPVRQPTQATITLKDASDNTINSWVYTMAAQQRR
                     FTAWFDLTGQKSGDYHVTVTVHTQEKAVTGQSGTVHFDQNKARKTPTNMQQKDTSKAT
                     TNSVDVDTKAQTNQSANQEIDSTSNPFKSATNHRSTSLKRSTKNEKLTPTASNSQKNG
                     SNKTKMLVDKEEVKPTSKRGFPWVLSGLVVSLAAGLFIAIQKVSRRK"
     gene            33733..34479
                     /gene="rlmB"
                     /locus_tag="VC122_000651"
     CDS             33733..34479
                     /gene="rlmB"
                     /locus_tag="VC122_000651"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010922667.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA
                     (guanosine(2251)-2'-O)-methyltransferase RlmB"
                     /protein_id="HEP5640595.1"
                     /translation="MEDKDTIETNDIVYGVHAVTESLQANTGNKLYIQEDLRGKKVDN
                     IKSLATQKKVAISWTPKKTLSQMTDGAVHQGFVLRVSAFAYTDVDEILEIAEQEANPL
                     ILILDGLTDPHNLGSILRTADATNVCGVIIPKHRSVGVTPVVSKTSTGAVEHIPIARV
                     TNLSQTLDKLKARGFWIFGTDMNGTPSDCWNTNGKLALVIGNEGKGISTNIKKQVDEM
                     ITIPMNGHVQSLNASVAAAILMYEVFRNRR"
     gene            34483..35001
                     /locus_tag="VC122_000652"
     CDS             34483..35001
                     /locus_tag="VC122_000652"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002982682.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NYN domain-containing protein"
                     /protein_id="HEP5640596.1"
                     /translation="MKKRILLVDGYNMIAFWQSTRQLFKINQLDQARNTLLTKLNHYA
                     HFENINIICVFDAQYVPGLRQRYDQYYISVVFTEEDETADSYIERMAAELNTAIHMVE
                     VATSDLNEQWTIFSQGALRVTARELEQRVHTVKADLDKMSRDIDLKTPKLRPFDQGQL
                     IQLKDFMSQLDR"
     gene            35098..35958
                     /locus_tag="VC122_000653"
     CDS             35098..35958
                     /locus_tag="VC122_000653"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011889153.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DegV family protein"
                     /protein_id="HEP5640597.1"
                     /translation="MTFTIMTDSTADLNQTWAEDHDIVLIGLTILCDGEVYETVGPNR
                     ISSDYLLKKMKAGSHPQTSQINVGEFEKVFREHARNNKALLYLAFSSVLSGTYQSALM
                     ARDLVREDYPDAVIEIVDTLAAAGGEGYLTILAAEARDSGKNLLETKDIVEAVIPRLR
                     TYFLVDDLFHLMRGGRLSKGSAFLGSLASIKPLLWIDEEGKLVPIAKIRGRQKAIKEM
                     VAQVEKDIADSTVIVSYTSDQGSAEKLREELLAHENISDVLMMPLGPVISAHVGPNTL
                     AVFVIGQNSR"
     gene            36094..36792
                     /locus_tag="VC122_000654"
     CDS             36094..36792
                     /locus_tag="VC122_000654"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015057286.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEP5640598.1"
                     /translation="MFDSKQNLAKSQWGFIVITAVMDLVVIVATLLAKTSFQRYLVGG
                     VAVILTSFLILLVWGLKTAKKLQTRLDSKEVLDSKVRDDQKLPRYDERQKQILLKGYT
                     IGFWFMIVAVWLSIFLHRFSERLVSASFLFTLALWGGLAVQTTYCNLNGVSPFVDRRF
                     GKQGILVGMIATGMSLITIGLAVREMMAGQLKMGSFFKAGGSGSLMVLGITLLSMGAS
                     IFYRHYLDAKEADE"
     gene            36789..36995
                     /locus_tag="VC122_000655"
     CDS             36789..36995
                     /locus_tag="VC122_000655"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003049560.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HEP5640599.1"
                     /translation="MKNLKLKAARAGKDLSQQALADLVGVSRQTIAAVEKGDYNPTIN
                     LCIAICRVLDKTLDDLFWEVDDSH"
     gene            37213..37659
                     /gene="rplM"
                     /locus_tag="VC122_000656"
     CDS             37213..37659
                     /gene="rplM"
                     /locus_tag="VC122_000656"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003083327.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L13"
                     /protein_id="HEP5640600.1"
                     /translation="MNKTTFMAKPGQVERKWYVVDATDVPLGRLSAVVASVLRGKNKP
                     TFTPHTDTGDFVIVINAEKVKLTGKKATDKVYYTHSMYPGGLKSITAGELRSKNAVRL
                     IEKSVKGMLPHNTLGRAQGMKLKVFVGGEHTHAAQQPEVLDISGLI"
     gene            37680..38072
                     /gene="rpsI"
                     /locus_tag="VC122_000657"
     CDS             37680..38072
                     /gene="rpsI"
                     /locus_tag="VC122_000657"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002942223.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S9"
                     /protein_id="HEP5640601.1"
                     /translation="MAQAQYAGTGRRKNAVARVRLVPGTGKITVNKKDVEEYIPHADL
                     RLIINQPFAVTSTEGSYDVFVNVVGGGYGGQSGAIRHGIARALLQVDPDFRDSLKRAG
                     LLTRDARMVERKKPGLKKARKASQFSKR"
     gene            complement(38205..38354)
                     /locus_tag="VC122_000658"
     CDS             complement(38205..38354)
                     /locus_tag="VC122_000658"
                     /inference="COORDINATES: protein motif:HMM:NF012798.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEP5640602.1"
                     /translation="MIEIGVDPVNTAKRLGHASSQITLDTYSHATAVGEDKSIKQFAN
                     YLKNN"
     gene            complement(38390..38752)
                     /locus_tag="VC122_000659"
     CDS             complement(38390..38752)
                     /locus_tag="VC122_000659"
                     /inference="COORDINATES: protein motif:HMM:NF012798.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HEP5640603.1"
                     /translation="MAALRKGELCALKWANIDFQSETLSVNKSLGRLDGQAIEKSTKN
                     DFSVRKIKLDSETISILQEWKAISQKEKAQLAVAPLSIESDFIFTYCTREGSIEPLHT
                     DYINNILSKVIKQHSLKK"
     gene            complement(38715..39209)
                     /locus_tag="VC122_000660"
     CDS             complement(38715..39209)
                     /locus_tag="VC122_000660"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002982723.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase family
                     protein"
                     /protein_id="HEP5640604.1"
                     /translation="MYLKASGNLEEFTKPTHKTYSEIFEEWYQAYQDTVEPTTASRTL
                     DLFQLHILPVMGELPINKTSPLDCQNFITDKAKTFKNIKQIKSYTEKIFDFAIKMKLL
                     KHNPMAEIIMPKRKKTRIENYWTVQELQEFLAIVLQKEPYKHYALFRLLAYSGLKERR
                     AMRS"
     gene            complement(39337..39672)
                     /locus_tag="VC122_000661"
     CDS             complement(39337..39672)
                     /locus_tag="VC122_000661"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003048651.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelE/ParE family
                     toxin"
                     /protein_id="HEP5640605.1"
                     /translation="MDYKKYQIIYAPDVLEKLKEIRDYISQNYSSTSGQRKMEQIISD
                     IEKLEVFREVGFDADEKYGSKIIHYHSTKGYTLSKDYIVLYHIEGEENRVVIDYLLPT
                     QSDYIKLFK"
     gene            complement(39662..39898)
                     /locus_tag="VC122_000662"
     CDS             complement(39662..39898)
                     /locus_tag="VC122_000662"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004261834.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEP5640606.1"
                     /translation="MVSEAMTVLNKKNLTLSSALRLFLQNVVVTNEVDLLTEEELEKE
                     KLFKQFQAEINKNIEDVRQGKFYTSEEVRAELGL"
BASE COUNT        11746 a         7533 c         8915 g        11758 t
ORIGIN      
        1 aatcttccga taaatagcta tttgattgaa tgttcttgct aaaaatgaat aacacttatt
       61 tagctttaat gattaaaaag acagaaaata caaaatcgtg ctgacaacaa accgaactgt
      121 gtgataaaga gtcatgtgtt ataatggttt aaagaccatc atgaaaggaa tggctatgga
      181 gctaaaagac tattttccag agatgcaagt aggccctcat ccgttaggag ataaagagtg
      241 ggtgagtgtc aaagaagggg accaatatgt gcattttccc aaatcatgtt tatccgaaaa
      301 agagcgctta ttacttgagg tggggctggg tcagtgtgag gtcttgcagc cgctagggtc
      361 accttggcag cggtatttgc ttgatcatca gggcaatccc cctcagctgt ttgagacttc
      421 tcagttcatt tacctcaatc atcagcaggc tttaccagat gacttagttg agttgctcca
      481 acagatgatt gcagggcttg aggtcatttt gcctatcagt gcaactcaga cggcattttt
      541 atgccgacaa gcaatctcaa taaaggtctt gcgctggctt gaagatctcc tgccaacgct
      601 agaaagtgat tttggtttgg ccttaaccat gtttgttgga aatgcctggt atcaagtagc
      661 tgcgggtaca ttgagagagt gttttgagga ggaatgtcag ctgttaacgg cctatttaag
      721 gcaacaatca ggcagaaaat tattaacatt ttcaggactg atgctgtggt ctttgctgtc
      781 gcaccacact tttttggcat tgacgcgtca gtttcaccaa tccctaagtc ctcaaagtga
      841 tatggccgat gtggttcacg cgctatggtc agaacacggc aatctggtgc agacagcgca
      901 gcgcttgtat attcaccgta actccttgca atacaaactt gataaatttg ctcagcaatc
      961 aggactccac ttgaagcaat tggatgattt agcctttgct catctctttt tgttgaaata
     1021 ttaggaaagc cacagagctt tcttttttgc tgcctggact gtagctgata aggagaatga
     1081 tagatcgttc agcttgtttt ggctcctgtg accggcctgt gtttacatag ttttcttggt
     1141 ttatggtccc ttacgacttg actagccctg ctattgtgca agttgcccaa agaagtttgg
     1201 tcgctttgtc tctgttttga aagcgctatt tttgttataa taaaagcatc atataaaaga
     1261 tatcagcggt ttctagcttt gcagtcagct agaacatttg ctggctaaat aggagttata
     1321 catggttgaa ttaaatctaa atcacatcta taaaaaatac ccaaatacta ctcattatgc
     1381 cgttgaagat tttgacttgg acattaaaga caaagaattt atcgtctttg ttggaccttc
     1441 aggatgtggt aaatcaacca ctcttcgtat gattgcaggt cttgaggaca tttctgaagg
     1501 agagttaaaa atcggtggcg aagtggtgaa tgacaagtct cctaaagacc gtgatattgc
     1561 catggtcttt caaaactacg ccctttaccc acacatgacc gtgtatgata acatggcctt
     1621 tggtttgaaa ttacgtaagt acaaaaaaga tgatattgac cgtcgtgtca aggaagctgc
     1681 gcagattctt ggtttgaccg aatttttaga gcgtaaacct gctgacctgt ctggtggtca
     1741 acgtcagcgt gtggctatgg gacgtgctat tgtccgtgat gctaaagtgt tcttgatgga
     1801 cgagcctttg tcaaacttgg atgcgaagct tcgtgtctcc atgcgtgctg aaattgctaa
     1861 aattcaccgt cgtatcggct caacaactat ttacgttacc catgaccaga cagaagccat
     1921 gactttggca gatcgtatcg ttatcatgag tgccactaaa aaccctcaag gtaatggaac
     1981 gattggtaaa attgagcaag tgggttcacc acaagaattg tataaccttc cagctaataa
     2041 attcgttgcc ggttttatcg gaagccctgc catgaacttc tttgaagtgg aagttaaaga
     2101 tggccgcatt gtgagtgaag atggtcttgc tattgcgatt cctgaaggtc aagcaaaaat
     2161 gcttgaagcg gctggttaca aggggaaaaa agtcactttt ggtattcgtc cagaagacat
     2221 ttctagtaga caaatcgtgc atgatactta tccaagtgca actgtcacag cagaagtttt
     2281 ggtttctgag ttactgggct ctgagacgat gctctatgtc aagcttggac aaactgagtt
     2341 tgcttctcgt gtagatgcgc gtgatttcca tagtccaggg gagcaagtta gcttgacctt
     2401 taatgtggct aaagggcatt tctttgacag agataccgaa caagctattc gttaaggtgt
     2461 aagcgatatt cataaaaagg ttgggttcca acctttttat catgtcattt gcgcaaacga
     2521 ttgcacaaaa agagaaaaaa cgttaaaata aaagatgaat gaatgattaa taggagaaga
     2581 ataatgaaga aacactggtg gcataaagct acgatttatc agatttaccc gagatccttt
     2641 aaagacacca gcggcaatgg tattggtgat ttaaaaggca ttaccagtca actggactac
     2701 ttgcaaaaac ttggcattac agccatttgg ttatctcctg tttaccaaag tccaatggat
     2761 gacaatggtt atgacatctc tgactatgaa gcgattgctg atgtttttgg agacatggca
     2821 gatatggatg agctcttagc agcagctaat gagcgaggca ttaagattat catggacttg
     2881 gtggtcaacc atacgtctga tgagcatgct tggtttgttg aagctaggga aaaccctaat
     2941 agtcctgagc gtgattttta tatttggcga gatgaaccta acgatttgac ttctattttt
     3001 agtggctcag cttgggaata tgataatgca tcaggccaat attatttgca cctttttagc
     3061 aaacgtcagc cagatctcaa ctgggagaat gaagctttac gtcataaaat ttacgatatg
     3121 atgaatttct ggattgctaa aggcatcggt ggttttcgta tggacgtgat tgacctgatt
     3181 gggaaggttc cagattctga aatcacgggt aacgggccgc gcttacacga ttacctcaaa
     3241 gaaatgaatc aagcaacctt tggcaatcat gacgtgatga ctgtcggtga gacttgggga
     3301 gcaacgcctg aaattgctcg ccagtattct cgccctgaaa acaaagagtt atccatggtc
     3361 ttccaatttg agcatgtggg tcttcaacat aaacctgacg ctcccaagtg ggattatgcg
     3421 gaagaactgg atgttcctgc tcttaaaact attttcagca agtggcagac agaattaaag
     3481 ttgggagaag gctggaattc gcttttttgg aataaccatg acttgccacg ggtcttatcc
     3541 atttggggca atgacagcac ttaccgagaa aaatcagcca aggctttagc tattttactg
     3601 catttgatgc gaggaacccc ttacatttac caaggagaag agattggcat gaccaattat
     3661 ccttttaaag acttaaccga ggttaatgat attgagtccc ttaactatgc taaggaagct
     3721 atgggaaatg gagtgtcagc tgcgcgtgtg atggatagca tccgtaaggt aggacgagac
     3781 aatgctagaa cgccgatgca gtggtctaag gacacacatg ctggcttttc tgaagcaaaa
     3841 gagacttggt tgccggtcaa ccccaactac caagacatca atgtggccga tgcccttgct
     3901 gatccagact ctatttttta cacctaccaa aaattgattg ccttgcgtaa ggagcaggac
     3961 tggctagttg aagcggatta tcacctcttg ccaacagctg ataaagtgtt tgcttatcag
     4021 cgtcagcttg gagaagagac gtatgtgatt gttgtcaatg tgtctgatga ggagcaagtc
     4081 tttgccaaag acttaacagg tgcacaagtg atcatcacca atacagatgt ggacacggtg
     4141 ttcgagacca agcacttaca accttgggat gccttttgtc tcaaactgaa ggcctagcag
     4201 ttgtatgtga tttgagttta cccaacagcc aagcgagtgg aaactcgttt tggctgtttg
     4261 atttttgaga tcctattaag gggtgatagt cattgtttga gctgtgttct aagttgaagt
     4321 gcggcatgtc ttgcaagcgc ttgcgcaatg tgataagata gtcttgtttg aatgttatac
     4381 taaatggagg tcttatgaaa aagaaagtca accaaggatc aaagcgctat caatatctgt
     4441 taaaaaagtg ggggataggt tttgtaatcg ctgcaactgg gactgtcgtg ttagggtgca
     4501 cccctagtat cttaacacat caagttgctg ctaaaaccat tgttggacta gcccgcgatg
     4561 aagctcaaca aggagatggc aatgctaaat ctggtgatgg tcttcaatcg tctagcaagg
     4621 aggcaaaacc agttttagac agctcgttag ctaatcctgc tagtattgct gagcatcatt
     4681 tgcgtatgca ttttaaaaca ttgccagctg gtgagtcgct aggaagcttg ggactttggg
     4741 tgtggggaga tgtggatcaa ccttcaaagg attggccaaa tggtgctatc accatgacaa
     4801 aagcgaaaaa agatgactat ggctattatc tagatgtgcc actagcagct aaacaccgcc
     4861 agcaagtgtc ttatctcatt aataataaag ctggagagaa tctttcaaag gaccagcaca
     4921 tctcgcttct cacaccaaaa atgaatgaag tttggataga cgagaattac catgcgcacg
     4981 cttatcgacc tttgaaaaaa ggttaccttc gaatcaacta ccacaatcaa tcgggacact
     5041 acgataactt agctgtctgg acctttaaag atgtcaaaac cccaacgacc gactggccaa
     5101 atggacttga cttgtcacat aaagggcctt atggagctta tgttgatgtc cccttaaaag
     5161 aaggagctaa cgaaatcgga tttttaatcc ttgataaaag taagacagga gatgctatta
     5221 aagtgcaacc aaaagattat ctgtttaaag agttagacaa tcatactcag atttttgtca
     5281 aagacactga cccaaaagtc tacaacaatc cttattatat tgatcaggtt agtctcaaag
     5341 gagctgaaca aaccacgcca aatgagatta aagccatttt tacgacctta gatgggcttg
     5401 atgaagatgc ggtgaaacaa aacatcaaga tcactgacaa agcagggaaa actgttgcaa
     5461 ttgatgagtt gacacttgac agggataagt ctgtaatgac attaaagggt gattttaagg
     5521 cgcaaggtgc agtctacacg gttacatttg gagaagttag ccaagtcgct cgccaatcct
     5581 ggcaattaaa agataaactc tatgcttacg atggtgaact tggagctacc ctagctaagg
     5641 atggttctgt tgatttagcg ctatggtctc caagtgctga tactgttaag gttgtcgttt
     5701 atgataaaca agatcagaca agggtggttg gtcaagctga tttgaccaag tcggacaagg
     5761 gtgtttggag agctcatctg acttctgata gtatcaaggg cattagtgat tacacaggct
     5821 actattacct ttatgaaatc acgcgcggtc aggaaaaagt catggttttg gatccttacg
     5881 ccaaatctct cgctgcctgg aatgatgcga ctgctactga tgacatcaaa acagcaaaag
     5941 ctgcctttat tgatccaagc aaactaggac caacaggcct tgattttgcc aaaattaaca
     6001 actttaaaaa gcgtgaagac gctattatct atgaagcaca tgtgcgagat tttacgtcag
     6061 ataaggctct agaaggcaag ttaacacacc cttttgggac tttttcagct ttcgttgaac
     6121 agctagacta tctcaaagac ttgggggtta cccacgttca attgctaccg gttttgagtt
     6181 atttttatgc caatgagctg gacaagagcc gctcaacagc ctacacgtct tcagacaata
     6241 attacaactg gggttatgac ccacaacact actttgccct ttctggcatg tattcggtaa
     6301 atcctaacga ccctgcttta cgtatcgcag agcttaaaaa ccttgtcaat gagattcaca
     6361 aacgtggtat gggtgttatt tttgatgtgg tttataacca cacggctaga acctatctct
     6421 ttgaagattt ggaacccaac tactatcatt ttatgaatgc tgatggcaca gctagagaga
     6481 gttttggcgg aggtcgtcta ggaacgacac atgccatgag tcgtcgtatc ttggtggatt
     6541 cgattgctta tctgactcgt gaattcaagg tagatggttt tcgtttcgac atgatgggtg
     6601 accatgatgc ggcagctatt gagcaagcct ttaaggcagc caaagccatt aatccaaata
     6661 ccattatgat tggcgaaggc tggcgtacct accaaggtga tgaggggaaa aaagaaattg
     6721 cggcagatca agattggatg aaagcaacca atacggtcgg tgttttctct gatgatatca
     6781 gaaataccct caagtcaggt ttcccaaatg aaggcacagc agcctttatt actggtggcg
     6841 caaaaaatct agaaggactg ttcaaaacga tcaaagcaca gcctagtaac tttgaagcag
     6901 atgccccagg agatgtagtg cagtatattg cagcccatga caacctgacc ttacatgatg
     6961 tcattgccaa atccatcaat aaggatccta aagtggctga agaagagatt cataagcgta
     7021 ttcgtctagg aaataccatg attttaactg ctcaagggac tgcctttatc cattctggtc
     7081 aggaatatgg acgaaccaag cagcttctaa atcccgacta caggacaaag gtgtctgatg
     7141 acaaggtgcc aaataaggcg actctgattg atgctgtagc gaaataccct tacttcatcc
     7201 acgattctta tgattcgtct gatgcggtca atcattttga ctgggcaaag gcaacagatt
     7261 ccatagctca cccgattagc aaccaaacaa aagcctatac acagggccta attgcgttgc
     7321 gtcgctcaac agatgccttt acaaaagcaa ccaaagctga ggtagatcgg gatgtgacct
     7381 tgatcaccca agcaggacaa gatggtattc aacaagagga ccttatcatg ggttaccaaa
     7441 cggtggcatc aaatggagat cgctatgctg tctttgtcaa tgcagacaac aagacccgca
     7501 aggtagtttt acctcaagcc taccgctatt tgctaggagc ccaagtgctt gctgatgctg
     7561 agcaggctgg tgttactgcc attgctaagc ctaagggagt ccagtttacc aaagaaggct
     7621 tgactattga tggcctaact gccctggtcc tcaaagtatc ctcaaaaaca gctaatccct
     7681 ctcagcaaaa gagtcagaca gacaatcatc aaaccaaaac accagatggc tcaaaagacc
     7741 tagataaatc attaatgact aggccaaaaa gagctaaaac aaaccaaaag ctcccaaaaa
     7801 cgggtgaagc ctcctcaaaa ggcttattag cagctggagt agctctgctt ttattggcta
     7861 ttagcctgtt gatgaagcgc caaaaagatt agggtggtgt taatagagac aaaaagattg
     7921 ctgtttgcgg attctttttg acggctataa aagctctgag ctgtgaaaag accttgtgat
     7981 tgagaaaatt gctactttag tgtatcatga gaccaaaaga gaaggagaca tccatgacat
     8041 ttgaagaaat cgttgcgaat tttataccgt cgagtgttgc agaagtcaca tcagctattg
     8101 ctagtggcaa agacatgatt gtctttttag gacgttcgag ctgtccttat tgtcgccgct
     8161 ttgctcctaa gttggcacaa gtggcaacag ataatcaaaa agaagtctat tttgtggata
     8221 gtgaaaacgc tgctgatgct gctgagcttg ctgcctttcg tgagaactac caattagtga
     8281 cagttccggc tttgctggtt agttacgatc agcaccaaag ggctgtatgt gattcaagtt
     8341 tgacaccaga tgacatctta gcttttttga ctcgtgagta aaggactgcg cttagcgtct
     8401 cgtttaaaga agaaatcagg taacgtctag tcttgctata cctcttgatt tttgatatag
     8461 taagacatga cttttataga ataaaaagga gacattatgg gatttccaac aattttaatg
     8521 tttgtagtga tgcttggatt gatttggttt atgcaacgtc aacaaaaaaa acaagcgcaa
     8581 gaacgccaaa accagttaaa cgctattgaa aaaggagacg aagtcgtaac gattggtggc
     8641 atgtttgcga ttgttgacga agtcgataca acagctaaaa agattgtgtt agatgtggat
     8701 ggcgtctttt tgacatttga gctatcagct attaagcgta ttgtaacaaa agctaccaca
     8761 gaaacgacac tggttgaaac agaagaaacc agtgttgtta atgagcctgc tgttgagaca
     8821 gtagatagca ctgacactga cagtgccatt gaaagccatt aaggcgggga aagtttggat
     8881 gaaatatgcc aaactttttt gttttgatcc ctggactgaa acagttattc tgatgctaac
     8941 aggttcatga aaagctgaca catctatcag aggataggtg aacattttgt gaagataaga
     9001 tctaattgat aaaaaacaag ccaaaaggcg tgaaatatga taaaatatga gataagtatg
     9061 cttatcaata aggaaaaaac atgtttggat taaaagcgaa atcaacaaaa aaagttttgg
     9121 ggagtatccc aaagcatatt ggaattatca tggatggtaa tggacgttgg gccaaaaaaa
     9181 gactaaaacc tagagtgttt gggcataaag ctgggatgga tgctttacaa gaagtaacga
     9241 ttactgcatc tgagttgggg gttaaagttc tgacagtcta tgccttttcg acagaaaact
     9301 ggtccagacc ccaagatgag gtgtctttta tcatgaattt accagtaacc ttctttgaca
     9361 aatatgtgcc tgtgcttcat gagaacaatg taaagattca aatgattgga gagactagtc
     9421 gtttacctga agataccttg gcagcgctaa atgcagccat cgacaaaaca aaacgtaata
     9481 cgggtttaat tttgaatttt gctctcaatt atggaggacg tgctgagatt accagtgctg
     9541 ttcgctttat tgctcaagat gtcttagatg ccaaattaaa tccaggagat attacggagg
     9601 atttgattgc taattacttg atgacagacc atctgccata tctctaccga gaccccgatt
     9661 tgattattcg caccagtggt gagctgcgtt tgagcaattt cttgccttgg caatcagctt
     9721 acagtgaatt ttatttcaca ccagtcttgt ggcctgattt taaaaaggca gagctactaa
     9781 aggccattgc ggattataac cgccgccaac gtcgttttgg caaggtctaa aaggagatag
     9841 ttatgaaaga acgtgttgtt tgggggggag tagcagtagc tattttccta ccttttctca
     9901 taataggaaa cctaccattc caattatttg taggcgtttt ggccatgatt ggggtatcag
     9961 aattactgaa aatgaaaaga ttagaagtct tttcttttga aggtgttttt gccatgttag
    10021 ccgcctttgt attggcagtt ccgatggacc attacctcac ttttttacca attgatgcga
    10081 atgttgcttt ttacagctta atggtttttt tcattttggc aggtactgtt ttaaatagca
    10141 gagcttattc atttgatgat gcggcctttc cgattgcaac tagtttttat gttgggattg
    10201 gttttcaaca tttgatcaat gctcgcctgt caggaattga caaggtcttt ttggccttat
    10261 ttatcgtttg ggccactgat attggagcat atctgattgg gcgtcagttt ggtaggcgca
    10321 aactattgcc tacagtttct ccaaacaaaa ccattgaagg tagtttagga gggatcgctt
    10381 gtgcagtcct tgtgtctttt atctttatgg tgattgatag gtctgtttat gcgccacatc
    10441 actttttgac catgttggtt ttggtcgctc ttttttccat ttttgcccaa tttggtgatt
    10501 tggttgaaag tgctctcaaa cgtcactttg gtgtcaaaga ttcaggtaag cttatcccgg
    10561 gacatggtgg tattttagac cgctttgatt ccatgatttt tgtttttccc atcatgcatc
    10621 tctttggttt attttaatct taagaggtaa tgtttggagt cgcaacaacg cgacaaaact
    10681 caaaaaagca atagactgaa aggaaatcta tgttaggaat aataaccttt attattattt
    10741 ttggtatttt agtgattgtc catgaatttg gacatttcta ttttgctaaa aaatcaggca
    10801 ttctagtaag agaatttgcc attggaatgg gccctaaaat tttttctcat gttgaccaag
    10861 gaggaactct ttataccttg aggatgttac ccttgggtgg ttatgtgcga atggctggtt
    10921 ggggtgatga caaaactgag atcaaaacag gtactccagc aagtttaacc cttaatgagc
    10981 aaggttttgt taagcgcatc aacttgtctc aaagtaagtt agacccaacg agtctcccaa
    11041 tgcatgttac aggctatgac ttagaagatc agctgagtat tactggcttg gttttagatg
    11101 aaaccaagac atataaggtc gctcacgatg ctactattgt tgaagaagac ggtactgaga
    11161 taagaattgc tccgcttgat gttcaatatc aaaatgctag tattggcgga cgtttaatca
    11221 ccaattttgc aggtcccatg aataatttta ttttagggat tgtggttttt atcctcttgg
    11281 tctttttaca aggtgggatg tcagatttta gtagcaatca tgtccgtgtt caagaaaatg
    11341 gagcagcagc taaggctggt cttcgagata atgaccaaat tgtcgcaatt aatggttata
    11401 aggtgactag ctggaatgat ctcactgagg ctgtagacct tgcaacacgg gatctaggcc
    11461 cgtcacagac cattaaggtc acctacaagt cacatcagcg cttaaaaacg gtggctgtga
    11521 aaccacaaaa gcatgcaaag acatacacga taggagttaa ggcgagtctg aaaacaggat
    11581 ttaaggataa gctcttaggc ggtctagaat tagcttggag tggggcgttt actattttga
    11641 atgctttgaa aggattgatc actggcttta gtctcaataa attaggtgga cctgttgcca
    11701 tgtatgacat gtccaatcag gctgctcaaa acggcttaga gtcagtctta tctcttatgg
    11761 caatgctttc gatcaattta gggatcttta acctgattcc gattcctgca cttgatgggg
    11821 gaaaaatctt gatgaatatc attgaagcca ttcgtcgcaa gcctatcaag caagaaacag
    11881 aggcctatat caccctagct ggggttgcta tcatggtcgt attgatgatt gctgtgacat
    11941 ggaatgatat catgcgcgtc tttttctaat caagataggt catcttaagc tgaccttatc
    12001 atgttaaaga tcgaaaaaga cctcaaccct aagatttgca agggttgagg tcttactcaa
    12061 aatgaaagga atacacattc aaattattga ataatcccgc ttcactctta cttgatgttt
    12121 ccagaagggt ttgaggataa ggggatgtat taaaacggaa taatctatga aacaaagtaa
    12181 actgcttatc ccaaccttgc gcgaaatgcc aagtgatgcc caggttatca gccacgcgct
    12241 tatggtgcgt gccggttatg tgcgccaagt ttctgctggt atctatgctt atttaccact
    12301 ggcaaatcgt accattgaga aattcaagac catcatgcgt gaagagtttg aaaagatcgg
    12361 tgctgttgaa atgttggcac cagctctttt gacagctgat ctctggcgtg aatcaggccg
    12421 ttatgagacc tatggagagg acctctataa gcttaaaaac cgtgataact cagactttat
    12481 cttgggtccg acccacgaag aaacctttac gactttggtg cgtgatgcgg tcaaatctta
    12541 caagcaattg cccttaaacc tttatcaaat tcagtctaag tatcgtgatg aaaaacgtcc
    12601 gcgtaatggc ttgcttcgta cccgtgagtt tatcatgaaa gacggctata gtttccatca
    12661 caactatgaa gatttagatg tgacctacga agattatcgt caagcttacg aagctatttt
    12721 taccagagct ggtctagatt tcaaagggat tattggagat ggcggtgcca tgggggggaa
    12781 agattcccaa gaatttatgg ccgttacacc agctcgtacg gaccttgatc gctgggtggt
    12841 tcttgacaag tctattgcgt caatggatga tataccaaaa gaggtcttag aagatattaa
    12901 ggcagaattg gctgcttgga tgatttcagg tgaagatact attgcttatt caacagaatc
    12961 aagctacgct gccaaccttg agatggcaac taacgaatac aaaccgtcct caaaagtagc
    13021 tgctgaagat gccttggcag aagttgagac accacattgc aaaacgattg atgaagtggc
    13081 tgcttttctt tcagtagatg aaacacaaac catcaaaacc ttgctttttg tggcagataa
    13141 tgaacctgtt gttgctttgc tagttggaaa tgaccatatc aataccgtta aattaaaaaa
    13201 ctatctagct gctgattttt tagagccagc tagtgaagaa gaagcccgtg ctttctttgg
    13261 tgcaggtttt ggctcacttg ggcctgtcaa cttggcacaa ggtagtcgca ttgtggctga
    13321 ccgcaaagtg caaaacctta ccaatgccgt tgcaggagct aacaaggatg gtttccatgt
    13381 gacaggagtc aatccaggac gtgacttcca ggctgaatat gtagatattc gtgaagtcaa
    13441 agaaggggaa atgtctccgg atggtcatgg tgttctccag tttgcacgtg gtatcgaagt
    13501 cggtcatatc ttcaagttgg gcactcgtta ttcagacagc atgggagcaa cgattcttga
    13561 tgaaaatggc agaacagttc caattgtgat gggttgttat ggtatcgggg ttagccgcat
    13621 tttgtctgct gtcattgaac agcatgcccg tctctttgtg aacaagacac caaaaggcga
    13681 ttaccgttat gcttggggta ttaacttccc taaagaatta gccccatttg acgtgcattt
    13741 gattacagtt aatgtcaaag atcaagtggc ccaagacttg acggcgaagt tagaagctga
    13801 cttgatggct aaagggtatg atgtcttgac agatgaccgt aatgaacgcg tcggttcaaa
    13861 attctctgat agcgatttga ttggtttgcc aattcgtgtc actgttggta aaaaagccgc
    13921 tgaaggtatc gtggaaatta aaatcaaggc aacaggtgac agcattgaag ttaatgcaga
    13981 aaacctcatc gaaacccttg aaattttaac aaaagaacac taaaaaagaa atgaagaggc
    14041 tgcctgttta gagtggcctc tttttgctgt gaacttgtct gatacagagt ggcttttgct
    14101 ttaccaaaag cgcttggcga caaaagattg gcaaaaaaat gacctcagct gagctatcac
    14161 ttggaataaa ttagccaaag aacgtcagaa gaaagccctg cttttctttc catctaaaac
    14221 tcgaaaatca tcttgaattg tgataaaata aagcctagta aaatcaggag aaaattatgt
    14281 cagatttatt cgctaaattg atggaccaga tagaaatgcc acttgacatg agacgttcaa
    14341 gtgccttttc atctgctgat attatcgagg taaaggtgca ttcggtgtca cgcttgtggg
    14401 aatttcattt tgcctttgcg gcggttttac cgattgcaac ttatcgtgaa ttacatgatc
    14461 gtttgataag aacttttgag gcggctgaca ttaaggtaac ctttgatatc caagctgctc
    14521 aggtggatta ttcagatgat ctgcttcaag cttattacca agaagctttt gagcatgcac
    14581 cgtgtaatag tgctagtttt aaatcttctt tctcaaagct caaagtgact tatgaggatg
    14641 acaaactcat tattgcagcg ccaggttttg tgaataacga tcattttaga aacagtcatc
    14701 tgcctaatct ggtcaagcaa tttgaagcct ttggctttgg caccttgacc atagatatgg
    14761 tgtcagatca ggagatgact gagcatttga ccaaggattt tgtttccagt cgtcaggctc
    14821 ttgtgaaaaa ggctgtgcag gataatttgg aagcccaaaa atcccttgaa gccatgatgc
    14881 caccagttga ggaagccaca cctgctccta agtttgacta caaggaacga gcagctaagc
    14941 gtcaggcagg gtttgaaaaa gcgaccatca caccaatgat tgagattgag accgaagaaa
    15001 accggattgt ctttgagggt atggtttttg atgtggagcg taaaacgact agaacaggtc
    15061 gccatatcat caactttaaa atgacagact atacctcctc gtttgctctg caaaaatggg
    15121 ccaaagacga tgaggagctc cgtaaatttg acatgattgc taagggagct tggttacggg
    15181 tacaagggaa tattgagacc aatcctttta cgaagagtct caccatgaat gtgcagcagg
    15241 tcaaagaaat tgtccatcat gaccgcaaag acctgatgcc agaagggcaa aagcgggtcg
    15301 aacttcatgc ccacaccaat atgtctacca tggacgcctt accgacagta gaaagcttga
    15361 ttgatacggc agccaagtgg ggacacaagg cggttgctat taccgaccat gctaatgtgc
    15421 aaagttttcc tcatggctac catagggctc gcaaagctgg aattaaggct atttttggcc
    15481 tagaagccaa tattgttgag gacaaggtgc ctatttctta tgatcctgtt gatatggatt
    15541 tgcacgaagc tacctatgtg gtctttgacg tggaaaccac aggtctatct gctatgaata
    15601 atgacctgat tcagattgcg gcttccaaaa tgtttaaagg caatattgta gagcagtttg
    15661 atgaattcat tgatcctggg catcctcttt ctgcctttac caccgaattg acagggatta
    15721 ccgataagca tttgcaggac gccaagccat tggttaccgt cctaaaagct tttcaggact
    15781 tttgcaaaga tagtatcttg gttgcccaca acgccagttt tgacgtgggc tttatgaacg
    15841 ccaattatga acgccacgac ttgcccaaaa tcacacagcc tgtgattgat accttagaat
    15901 ttgctagaaa cttgtatcct gagtacaagc gtcacggttt gggaccgctc accaagcgtt
    15961 tccaagtgag tctagaccac catcatatgg ccaattacga cgcggaagcc acaggacgtc
    16021 ttttgtttat ttttctaaga gatgccagag aaaagcatgg catcaaaaat cttttgcaac
    16081 tcaatacaga tttggtagct gaggattctt acaaaaaagc gcggattaag catgcgacta
    16141 tctatgtgca aaatcaggtt ggccttaaaa atatgtttaa gttggtcagc ctttccaata
    16201 ttaaatattt tgaaggggtg ccgcgtattc caagaaccgt cttagatgct cacagagagg
    16261 gtttgttact tggtacagct tgttctgacg gcgaggtttt tgatgccgtt ctgactaaag
    16321 gaattgatgc ggcggttgat ttggctaagt attatgattt tatcgaaatc atgccaccag
    16381 ccatttacca gccattggtt gtccgtgaat taatcaaaga ccaagcaggt attgagcagg
    16441 tgattcgtga cctcattgaa gtagggaaac gagctaataa acctgtactt gccactggaa
    16501 atgtgcatta cctagagcct gaagaagaga tttaccgtga aattattgtg cgtagtctcg
    16561 gtcagggtgc catgattaat agaacaatcg gccgtgggga aggggcccaa cctgctcccc
    16621 tacctaaagc gcactttaga acaaccaatg aaatgctgga tgagtttgcc tttcttggaa
    16681 aagacctcgc ttatcaagtg gttgtagaaa atactcagga ttttgcggac cgtattgagg
    16741 aagtggaagt ggttaagggc gatctttaca ccccgtatat tgacaaggcc gaagagacgg
    16801 ttgccgaatt aacctatcaa aaagcctttg aaatttatgg taatcctctc ccagatatta
    16861 ttgatttacg cattgaaaaa gagttaacct ctatcttggg gaacggtttt gctgtgattt
    16921 atctcgcttc ccaaatgctt gttaaccggt caaatgagcg aggctatctg gttggctcta
    16981 ggggatctgt agggtctagc tttgtcgcca ccatgattgg gattactgag gttaatccta
    17041 tgccgcctca ctacgtttgc ccgtcctgcc aacattctga atttatcaca gatgggtcag
    17101 ttggatctgg ctatgatttg cctaataaac cctgtccgaa atgtggcacc ccttatcaaa
    17161 aagatgggca agacattccc tttgagacct ttcttgggtt tgatggggat aaggtgcccg
    17221 atattgattt gaacttctct ggtgatgacc agcccagtgc ccatttggat gtccgagata
    17281 tttttggtga cgaatacgcc tttcgtgctg gaacagttgg taccgtagca gaaaaaacag
    17341 cttatggatt tgtcaaaggc tatgaacgcg actatggcaa gttctatcgt gatgctgagg
    17401 tggatcgtct agcagcaggt gctgctggtg tgaaacgaac gactgggcag caccctgggg
    17461 ggattgttgt tattcctaat tacatggatg tttatgattt tacccccgtg caatatccag
    17521 cagatgatgt aacggcttct tggcagacaa ctcactttaa cttccatgat attgatgaaa
    17581 acgtcttgaa acttgatatc ctagggcatg atgatccgac catgattcgt aaacttcagg
    17641 atttatcggg cattgatcct attactatcc ctgctgatga tccaggtgtt atggctctct
    17701 tttctgggac agagattttg ggcgttaccc cggaacaaat tgggacaccg actggtatgc
    17761 taggcattcc agaatttgga accaactttg ttcgcggcat ggttaatgag acgcatccga
    17821 ccacttttgc ggagcttttg cagttgtctg gactatctca tggaaccgat gtttggcttg
    17881 gtaatgcaca agatttgatt aaagaaggca ttgcgaccct aaaaaccgtt atcggttgtc
    17941 gtgacgacat catggtttac ctcatgcacg caggcttaga accgaaaatg gcctttacca
    18001 ttatggagcg tgtgcgtaag ggcctctggc tgaaaatttc tgaggaagaa cgtaatggct
    18061 atattgatgc catgcgagaa aacaatgtgc ccgactggta cattgaatcg tgtggaaaaa
    18121 tcaagtacat gttccctaaa gcccatgcgg cggcctatgt cttgatggcc cttcgggtgg
    18181 cttatttcaa ggtgcaccac cccattatgt attattgtgc ttatttctct attcgtgcga
    18241 aggcttttga attaaaaacc atgagtggtg gcttagatgc tgttaaagca agaatggaag
    18301 atattactat aaaacgtaaa aataatgaag ccaccaatgt ggaaaatgac ctctttacaa
    18361 ccttggagat tgtcaacgaa atgttagaac gcggctttaa gtttggcaaa ttagaccttt
    18421 acaaaagtga tgctatagaa ttccaaatca aaggagatac ccttatccct ccatttatag
    18481 cgttagaagg tctgggtgaa aacgtggcca agcaaatcgt taaagctcgt caagaaggcg
    18541 aattcctctc taaaatggaa ttgcgtaaac gaggcggggc atcgtcaacg ctcgttgaga
    18601 aaatggatga gatgggtatt ttaggaaata tgccagaaga taatcaatta agtctttttg
    18661 atgacttttt ctagattatc atgatggtag tcatatgcaa agtcataaaa gaagagggac
    18721 aagatcactt tctaatctct ctgatactaa attgtgacat accgtataaa gcgaggaaat
    18781 caaatgagtc atttagataa gaacaccgct ttgaaagcca tggtggtgtt tcgtaaagct
    18841 cagcgaacgc tagatgcttt tggggctgat atttttaaaa aggcagattt aacagccacg
    18901 cagttcagtg tgcttgaagt actatatact aaaggctgta tgcgaatcaa tcacttgatt
    18961 gattctttgt tagcgacttc ggggaatatg actgttgtac taaataatat ggaacgtaat
    19021 ggctggatta gcaagtgcaa agacaaaacg gataaaagag cctatgtggt taccttaaca
    19081 gataagggta ctcgcttgat tgaagctgtt ttgcctaaac acgtcgcacg ggtggaagaa
    19141 gcctttgctg ttttgactga aaaagaacaa ctttgtctga ttgaacttct caaaaagttc
    19201 aaacaattat aaacaaatct ctttacagct cttcttagag aagatataat tactacatag
    19261 tgataaattt caaaaagaag tatcaatgaa ataccacttt tagaaagctt taaaacgatc
    19321 tttaaaaaaa gaggacaaag agatgaaaca tattttattt attgttggct cgcttcgtga
    19381 agggtctttt aaccatcaat tagcggctca agcacaaaaa gctctggaac atcaagcagt
    19441 agtatcttac ttaaattgga aagacgttcc tgttttgaat caagatatcg aagctaatgt
    19501 acctttacca gttgttgacg ctcgtcaagc tgttcagtca gcggatgcta tctggatttt
    19561 tacaccagtt tacaacttct ctattccagg ttctgttaaa aacctgctag actggttgtc
    19621 tcgtgctctt gatttgtctg atccgacggg cccatctgct attggcggta aggtggttac
    19681 ggtctcttca gttgcaaatg gcgggcatga tcaagtattt gatcagttta aagcactatt
    19741 gccgtttatc cgaacttcag tagcaggaga gtttacaaaa gcaactgtga atcctgatgc
    19801 ctggggaaca ggaaggcttg agatttcaaa agagacaaaa gcaaacttgc tatctcaggc
    19861 agaggctctt ttagcggcta tttagactaa caaaaaaagg ctaaagggcc ttttttgtta
    19921 gtctaaaatc aagagctctt ctttggtttc aaaggggttt ttagcattga tccgatcgta
    19981 gaacaaaaca ccattgatat ggtcaatttc atgttgaacc acaatagcgt tgtatccctt
    20041 taatttgatg cgatgctgtt gtccttcttt atcatagtag tctactgtaa cacgagcatg
    20101 gcgaaccaca tacccttcaa caacacgatc gacagataaa caaccttcgc catcagaaag
    20161 ggctgcatct tgaacagaat gagaaacgat tttaggatta tacaggactt cttgccagct
    20221 ataggcttct ttaggaggat ttccttcttt atcaggaaga ttaggaacga gcacagcgat
    20281 gatacgtttt gacacatcaa tctgaggagc tgctagacca acaccggccc taagtccaag
    20341 tttttccgcc attacaggat cttgagaatg cttcaaaaat tgcatcatct tttctcctaa
    20401 taggatatcc tcatcacaca agggtaagct cacttctttg gcgacagctc ttaaagttgg
    20461 attcccttca cgaataatgt catccatggt aatcagatga ctaggtttaa ttaatttatc
    20521 ttgtgcagac atgtctactc ctttactttt ttttaactgg atttactata tcacaaatca
    20581 tttctttttt aaagaaaagt caggtagtgg tcagagtgat tgctattata agttaatggg
    20641 ctgcatacct cagaggtcta gttattaaca cttaattgag ttagagcgtc taaatcggca
    20701 atacgacagg ttttgaagca gatgcgttcg ataatcctct gaaaaatggg ctgcttaagt
    20761 acgtgcttaa gatggcgatc gcttgttcca aaacggtttg ctaataatgt aaggtttaga
    20821 tgaacctctt ggttggcttg cgcttcaagg gtataggaag caaagcgctc tttgacggta
    20881 taggtaatgt tttgttggca aacattttgt gtcaactctt gacctatgtc ataaaggcag
    20941 gtcgcatcgg ttaataagct gtgttcatgg attagggatt gggtttgtta atactagagc
    21001 aattaatatt atcagtgaac actttgttgg aaagaaatga atgactatgc taggttaccg
    21061 cttatcagct gaagtgcggg gatcagccat cttacctctt ttagctggtt atcttattag
    21121 gcaagcaatc gcacaggttt attctgattt aaaaacagcc tcttggctat ttggccttgc
    21181 tagttctgtt gcttaaggtg gtttggatgt gagagcttag aataatagga taacggttga
    21241 cctaatatta tgaatctgct agaatagagt ggtcgaaaga caaaccaact ttttcttggt
    21301 tttaggagtg ccaatcctaa gactcggatt aagcaaaaaa aggagaatat attatggcaa
    21361 tctcaaaaga gaaaaaaaat gaaatcattg ctcaatatgc acgtcacgaa ggcgatactg
    21421 gttcagttga agttcaagta gcagttctta catgggaaat caaccacttg aacagccaca
    21481 tcaaagaaca caaaaaagac cacgctactt accgtggatt gatgaaaaaa attggtcacc
    21541 gtcgtaactt gttggcatac ctacgtcgta cagatgttaa ccgttaccgt gagttgatcc
    21601 aatctcttgg acttcgccgt taatttcaag tttaaaaagt cctctgctta tgcagtgggc
    21661 tttttcattt ttgacatgtt gtttgttaac ttacacattt ggctagaatg gtctaagact
    21721 gtttttttaa gcagttgatg attttattta acaacaaaag gtatctcagt gatgaggtat
    21781 cttttgttgt atcatgaaga aaaagggaat caattcccag atttgcctat ttttggccac
    21841 cttttgagga acttgttaca atgatatcac ctgttgagtc caattgcagt caaaaatgac
    21901 tacggacaat tggactcaat ttgtcttgaa aatgaaaacg cttcatttta tactgacatt
    21961 ataggaggag atgacatgct atctcatgag ctcatcagaa actatcaact cttttctaaa
    22021 tataaaggac attcactgga ggcatttgaa tccatattaa agacaagcaa acgtcatata
    22081 ctggcagata ttgctaaaat caatgacacg ttgtcactct atcagttacc ccttattgct
    22141 ctggacaggc agctggttta tccgccagac cttactgaaa aggacttgtt gaatcgcatg
    22201 ctacctacct tagacgacta tctctttcaa gatgagcgtc tggacatgat tatcatttac
    22261 atcatgatgg ctaaagaatt tatctccatt aaccacttgg aaagcttgtt acggctcagc
    22321 agaaattccg ttattgctga tttaaacttg gtgcgtgatc gggtacaagc ttttcaggta
    22381 actttagctt acaatcgcca ggatggttat ttttttgaag gagaaccctt agccttgcgg
    22441 cgtctcttag aatcagcggt cagttctctt ttgcaagtta catcaggacc ttgggtgttc
    22501 agttacttat tgcatgaact tggtttgccc gaccagacaa aggtgatggc agcaacgttg
    22561 gaggaactga gcagggagaa ccacttaact tttatttcgg agaaattgag ggatttgatt
    22621 tattttttct gccttctcgc tcatcgacca ttttcacgga acgtaagggc tgaagtagtg
    22681 gatacttttc ctttagcttc gccagctgtt gaaacgatgg tggaccagtt attggtcaac
    22741 ttccccagtc taaccgaaga gaaatatttg gttcaatcta ggttacttgg ttgtatccaa
    22801 ggtgacttag agttagtctt tcagcaacct atttatgaca ttatggagga aatcatcaat
    22861 tcggtggcgg ttaacacggg attgtctatc accgataccc cagaacttcg tcagaaccta
    22921 tacagtcacc ttttgccagc ttattaccga ctttactacg acattaactt gaccaatcct
    22981 ttgaaggaac aaatcaaaca agattatgaa tctctctttt acttggtcaa gcgcagcctt
    23041 tctcctctag aaaaacaatt ggggaaacca gttaatgaag atgagattgc ttattttacc
    23101 attcattttg ggagatggtt gcaggcccct aagaagaggc cgagcaatca gctggtagcc
    23161 ttatctgttt gccctaatgg catcagttcg tctctgatgt tggaggcaac cttgaaggaa
    23221 ctttttccac agctacagtt tattaggatt caccagctgg acaaaataaa gttgttggat
    23281 ccagcttcct ttgatttgat tttttcaaca gtagcttttg actgtgttaa acctgtttat
    23341 gtgacgcaag ctttgatggg acctgttgaa aaaatgatgt tgaaaaagat ggtctgtgat
    23401 gactttcatc ttcccttgtc agagcaattc gctttggatg acctgttgag tattattcat
    23461 aaacacacca cgattactaa taaagaagga cttgttagtg atttatcccg ttacctgttt
    23521 ggtaaccatt taacgattga aaaaggaggt ctaggactat tggacttgtt aacagcagat
    23581 tttattaggc aggctgatgc tgtttcggat tggcaggaag cgattcgttt ggctgctcag
    23641 cctttactag aacaccagat gattgaaaca tcttatatcg acggtatgat tgattcggtc
    23701 aatgagcttg gagcctatat tgttttagcc cctaaggtgg ctgttcctca tgcggcgcct
    23761 gaaaaaggaa cgcggcagtt aggcatgtct ctcttacaac taaaagaacc tgtcagcttt
    23821 gatttgaagc aagaaggtga tccagacaag caagtgcaat tgatttttgt tttgtctgct
    23881 gtggattcta gttcacattt gaaggctttg caggaactgt cgttgatttt agatgatgat
    23941 gaacatattg agcaattaat tgaggctaag aatactgagg aaataatgag tctgattagc
    24001 catatgattg aaaaaggaga tgaatcacat gattaaaatt gtaacggttt gtggaaacgg
    24061 tattggtagt agcttgttac ttcgcatgaa agtagaagct atcgcctcta gtttgggtat
    24121 tgatgtggat gcagaatcct gtgattccaa tgcggctgtt ggaaagggtg cagatttgtt
    24181 tgttaccgtt aaagaattta aagatatttt tccagaggat gccaaggttt gtatcgttaa
    24241 aagctatacc aatcgtaaaa aaattgaaga agatttggtt ccggttctca aggaaatgag
    24301 tggcaaggaa taagccttta aaggagatag gaagatacta tggaagcatt attatcattt
    24361 attcgagata ttttaaaaga acctgcattt ttaatgggct tgattgcctt tgcagggttg
    24421 gtggctttga aaacacctgc tcataaggtg ttgacaggaa ccttggggcc gattttggga
    24481 taccttatgc ttgctgcagg ggcgggtgtt attgtgacca atctggaccc tcttgccaaa
    24541 ctcattgagc acggttttag catcactggc gtagtgccaa ataatgaagc cgtcacttct
    24601 gtcgctcaaa agattctggg tgtggaaact atgtccatct tggtggttgg gttattgctt
    24661 aatttggctt ttgctcgttt tacccgcttc aaatacattt tcttaacagg acatcacagc
    24721 ttctttatgg cttgtctctt gtccgccgtg cttggagctg ttgatttcaa aggaagtctt
    24781 ttgattatct taggtgggtt cctcttggga gcttggtcag ctatttcgcc agccattggt
    24841 caacagtata ctctgaaagt gactgacgga gatgaaatcg ccatgggaca ctttggtagt
    24901 ttgggctatt acctttctgc ctgggttggt agcaaggttg gcaaagacag taaagatacc
    24961 gaggaccttc agatttctga aaaatggagt ttcttacgca acaccaccat ttcaacagga
    25021 cttatcatgg tgcttttcta cttggtggca acagtggctt ctgttttgag gaatacttca
    25081 gtaacagaag aattagcagc aggtcaaaac ccatttatct ttgctattaa gagtggtctt
    25141 acctttgcgg ttggtgtggc aattgtctac gccggtgttc gcatgatttt ggctgacttg
    25201 attccagcct tccaaggtat tgctaacaaa ttaattccaa atgctattcc agccgtggat
    25261 tgtgcggtat tcttccctta tgcgccaacc gctgttatca tcggttttgc gtcaagtttc
    25321 gttggtggct tacttgggat gttgatttta ggagttgcag gtggtgtcct gattatccca
    25381 ggtatggtgc ctcatttctt ctgcggtgca acagcaggga tttttggaaa ttcaacaggt
    25441 ggtcgtcgcg gtgcaatgat tggtgctttc gctaatggct tattactcgc cttcttgcca
    25501 gccatgcttc tacctgtact tggtaaactt ggtttttcaa acacgacctt tggagatgtg
    25561 gatttcggtg ttttagggat tttactgggc cgcttaggaa cgtcgattgg tcaaatcggt
    25621 atttacttgg tggttgctgt gcttgctatt gtattggtgc ttccaaactt tctgtcaaaa
    25681 tctcaagaag ccatcaataa tgtttcagaa gaaatctaaa aggaggatat gatgacaaaa
    25741 aaactaaacg ttaaagtctt ttcagacggt gcggtcttag agaccatgtt aaaggattta
    25801 caaacaggtt tggtcacagg ctttacaacc aatccaagtt tgatgaaaaa agcaggcatc
    25861 agttcttata ttggctttgc caaagaagtg cttgccaaaa ttacagacta cccagtgtcc
    25921 tttgaagtct ttgcggatga tctcgcaagc atggaaaagg aagctgagaa aattgccagt
    25981 ctaggtgaca atgtctatgt gaaaattcca gtcaccactt caacaggcga atcaacttgt
    26041 cccgttattc aaaagttgtc agccaaaggc attaagttaa acgtcacagc catttttaca
    26101 attgagcaga ctcaagctgt ggttgatcat ttaaccgctg gagtgccagc tattgtgtct
    26161 gtttttgctg gtcgtattgc agacaccggc gttgacccaa tgccaatcat ggaagaagct
    26221 ctacgcattt gtcgtcaaaa agaagaggtc gaattgttgt gggctagtcc tcgtgaaact
    26281 tataatatct atcaagctga tcagttaggc gtggacatta tcacttgtac cactgatttg
    26341 attgccaagt tgcctcttca aggaaaagat ttagaagact attcccttga aaccgtgcaa
    26401 atgtttctga aagacagtac cagcctcggc tttaaaattt tagaggatga caatcattaa
    26461 tattaggaga cgaaagcagg cataccaacc tgcttttttt gctttgttaa agagtataag
    26521 tttcgtttaa tatttttcaa ggacatttca agagattgcg atctgacagt gaaatttctg
    26581 ctattttatg gtaaaatgta gcagatacgt cgtttgatac aaagaggagc gtgtgacaag
    26641 cgtggcaggt tgctttttgt agcaaaacaa cgtataacaa ctgaatctaa gcagagaaca
    26701 ggtacctctt tacccctctg aaaattaaaa ggagaatata tgtcaaaaca aacctttaca
    26761 acaacatttg cagggaaacc ccttgttgtt gaagttggtc aagtcgctaa gcaagccaac
    26821 ggggcaaccg ttgttcgtta cggagaatca accgtcctga ccgcagctgt tatgtctaag
    26881 aaaatggcaa cgggtgattt cttccccctt caagttaatt acgaagaaaa aatgtatgcc
    26941 gctggtaaat tcccaggagg tttcatgaag cgtgagggac gcccttcaac agatgcgacc
    27001 ttgacagctc gcttgattga ccgcccgatt cgtcccatgt ttgccgaggg ttttcgtaac
    27061 gaagtacaag tgattaacac tgtcctttct tatgatgaaa atgctagcgc tccaatggca
    27121 gcgatgtttg gctcatctct ggccttgtcc atctcagata ttccatttaa tggaccgatt
    27181 gcaggtgttc aagttggcta tattgatggt gagttcatca ttaacccaga taaagaacag
    27241 atggaagcgt cactcttaga attaaccgtt gctggtagca aagaagccat caacatggtt
    27301 gaatctggtg ccaaagaatt atcagaagac atcatgcttg aggctctttt aaaaggtcac
    27361 caagccattc aagaattaat tgccttccaa gaacaaatcg tagcagttgt tggtaaagag
    27421 aaagctgagg ttgaactgtt acaggttgat gcagacttac aagctgatat tgtggctaag
    27481 tacaatgctc aactgcaaaa agctgttcaa gttgaagaga aaaaagcgcg tgaagcagcc
    27541 acagaagccg ttaaagaaat ggttaaggct gagtacgaag agcgttacgc agaagacgaa
    27601 aacctagcaa ccattatgcg tgacgtggca gaaatccttg aacaaatgga gcatgctgaa
    27661 gtgcgccgcc tcattacaga ggacaagatt cgtcctgacg gtcgtaagat tgacgaaatt
    27721 cgtccgcttg atgctgtggt tgacttcttg ccaaaggtcc atggatcagg tctttttacg
    27781 cgcggtcaaa ctcaggcgct atcaattttg accttggcac caatgggaga aactcaaatc
    27841 attgatggtc tggccccaga gtacaaaaaa cgcttcttgc atcactacaa tttcccgcaa
    27901 tattcagttg gtgaaacggg tcgttacgga gctgctggtc gtcgtgaaat tggacacggt
    27961 gccttaggtg aacgcgctct tgaacaagtc ttgccaagct tagaagagtt tccttatgcc
    28021 attcgtttag ttgccgaagt tctagaatct aacggttcgt cttctcaggc ttctatctgt
    28081 gctggtaccc ttgctctgat ggctggtggt gtaccaatca aggctcctgt ggcagggatt
    28141 gccatgggtc tcatttcaga cgggacgaat tatacagtct tgaccgatat ccaaggtctt
    28201 gaagaccact ttggagatat ggactttaaa gtggcaggaa ctcgtgaagg gattacagcc
    28261 cttcaaatgg atattaagat tgcagggatt acgcctcaaa tcctagaaga agctcttgca
    28321 caagctaaaa aagctcgctt tgaaatcctt gatgtgattg aagcaactat cgctgaacca
    28381 cgtcccgagt tagccccaac tgcaccgaaa attgacacca tcaagattga cgtagacaaa
    28441 atcaaagtgg ttatcggtaa aggtggcgaa accattgata agattattgc cgaaacgggt
    28501 gtcaaaattg atattgatga cgaaggaaat gtctctatct actcaagtga ccaagctgcc
    28561 attaaccgca ctaaagaaat catcgctggc ctagtacgtg aagctaaggt aggcgaagtt
    28621 taccatgcta aggttgtccg tattgagaaa tttggtgcct ttgtcaacct ctttgacaag
    28681 acagatgcac ttgttcacat ttctgaaatt gcttggacta gaacgaccaa cgtgtcagat
    28741 gtccttgaag tgggtgaaga cgttgatgtt aaggtcatta agattgatga gaagggtcgt
    28801 gtggacgcct caatgaaagc tttgattcca cgcccaccaa aaccagagaa aaaagaagag
    28861 aaacatgact aaatcaaatg aattagatat tcgcttaaga gcttttatca atgcgcctga
    28921 taactttttg gatagtgttg cccttgtcaa tgctttccat aattttcctg tctgggctgc
    28981 taaagaaccc tacgtgatag aagtcgaagg tgtcaaggtt acccctgttt ttaccgataa
    29041 ggaagatatg gctcgcttta aagaagaaca agagagtgct caaagtcagt attggctgga
    29101 gcgttcagct cttgcagtgc tagaagaagt cattacatct ggtgcagcag gtcttgtctt
    29161 taaccttaag aaaaagggag attttgggaa ttctaccatt ttcaaaagca gtgatatgat
    29221 tcaatttatg aaccattaca ccactgtgtt aaatacgctt atgagtgatg acaatgtggc
    29281 ggcagatacg atggaaaagg tttaccttgt tcctgccttt gtttatccta aagataataa
    29341 ccattacgac cgcctttttc ctaccatgtc aacgcctgaa ggaaaaagct atgttcccgc
    29401 cttttcaaat cttcaaagct ttgcgaaatg gtacaaccaa gacgattttg gaggcctttt
    29461 caggaaagct gaaggagtga ttctgacttg gacgattgat gatatttatc aaccaagaaa
    29521 tggtgaaaac gagcttgatg aaacgttcgg tgtcgccatc aatccttttg atgaccaaca
    29581 aattcttgtt gattggtcag aattagataa gtcgtaggag ttagtatggg ttggtggaaa
    29641 gaaagtatag ctattgttaa agctctagac ccagctgctc gtaatagtct tgaggttatc
    29701 ctaacttatc caggcatcaa agccctagct gctcatcgtt tgtctcattt tctttggcga
    29761 catcatttta aattactagc cagaatgcat agccaatttt ggcgattttg gacacaaatt
    29821 gaaatccatc ctggagcaca aatcgctcct ggagtcttca ttgaccatgg tgctggtctt
    29881 gttattggag agacagcaat tgttgaaaaa ggtgtgatgc tttatcatgg ggtgacccta
    29941 ggtggaaccg gaaaagattg tggcaagcgt catccaacgg ttcgacaagg tgccttaatt
    30001 tcggcacatg cccaagtgat tggacctatt gacatcggag caaatgctaa agtaggggca
    30061 gcagctgttg tgttatcaga tgttcctgaa gacgtgacag ttgtaggtgt gccagctaag
    30121 atagtacgag tgcatgggca aaaagataat cgtcaaattc aaagtttaca aaaacaacga
    30181 gaggtctctt atcagttgtc aaaatgatcg gaaaataaca aaaagaagca ctgatgactt
    30241 ccgataaaat attttgggac tttctagtag agtaatgttg aacttagcta gccctatcag
    30301 tttggttgct gagtgtagtg atatgggaat gttacaaaat acaaaaaaca cgtaaaagag
    30361 catcaactaa gcaagtctta gactgaagaa acagtctatt gtgatacttt ttctcaggtg
    30421 gtgcttacgg cagcgacttc ctttggaatt ccatgcctta gttttgagcc taaggtctca
    30481 aaaatctgta tgggcatcac tgtcagtatt gataccactt ttaccacaac aaaaagcagc
    30541 tgttttggct caccccacat gcagaaatag gaaacctgat gtcaaatcag cagatctatt
    30601 catgcttttt gagcataaaa gtgggtttgt ctactttctg aagccttcac aggttgtgaa
    30661 ggtttttcgt taataaagga gcctcatgaa taaaataaga gttgggctgt atgtcattat
    30721 gagtttgtct attcttgttt ctagtattac actggtggct agtctaatct ttttactgag
    30781 acaagacatg gttaaagcga ttatatactt cattttgacc ctattttggt taataggttt
    30841 ggttattaat attaaattgt tgaaaaggtg gaatagcatt tcagaaaaag acttaggata
    30901 tcaagatgac aactaatgct aggatattga ataaggagtt tacatgatta aaatttatga
    30961 taccatgacc cgttcgctcc gcaagtttgt acctttgact gaaaatacag tcaatatgta
    31021 cgtttgtgga ccgacggtct ataattatat tcatattgga aatgctagat cagcggtcgc
    31081 ttttgacacc attcgacgtt attttgagta tactggctat caggtcaatt acatttccaa
    31141 ttttaccgat gtcgatgata aaattatcaa ggctgctact caagcaggtg tttctcctaa
    31201 agaattgtca gatcgcttta ttgcagcttt tatagaagat accaaggcac ttggtgttaa
    31261 gccagccaca caaaatcctc gtgttatgga ttatatagca gaaatcattt catttgttga
    31321 aagcctcatt gaaaaagatt ttgcttacga agcagatgga gatgtgtatt tccgcgtgga
    31381 aaagtcagag cattatgcca agctagctaa taaaaccctg tcagaacttg aagttggagc
    31441 cagtggtcga acagatgctg agacagcttt aaaagaaaat ccactggact ttgctctttg
    31501 gaaatcagct aaggcaggtg aggtttcttg ggatagcccc tgggggtttg gtcgtccagg
    31561 ctggcacatt gaatgttctg tgatggctac tgaaattctt ggtgatacca ttgatattca
    31621 tggtggcgga gctgatttgg aatttcctca tcataccaat gaaattgccc aatctgaggc
    31681 taaaacaggc aagacttttg ctaactattg gatgcacaat gggtttgtca ctgttgataa
    31741 cgaaaaaatg tccaagtcac taggtaattt tgtgaccgtt catgacatgt tacaaaccgt
    31801 tgatggtcag gttttgcgat tctttcttgc gacacaacag taccgaaaac caattaactt
    31861 cactgaaaag gctattcatg atgcggaaat caatctgaag tatcttaaaa acaccttgca
    31921 acaaccgctg acagaaactg cagatgagca agagctaaaa cagtttgtaa tagctttcca
    31981 agacgctatg gacgatgatt tcaatacagc aaatgggatt accgtcgtat ttgacatggc
    32041 taagtggatt aattctggct cctatacaga acctgttaaa agcgcttttg agaaaatgtt
    32101 agctgttttt ggcattatct ttgaagaaga ggtgcttgag gttgacattg aagccttgat
    32161 tgctaagcga caagaagcac gcgcgaatcg tgattttgcc atagctgatg ccattcgaga
    32221 tcagttagca gctcaaggca tcaagctgct agataccaaa gatggtgtga ggtggctgcg
    32281 tgattaatcc agttgatgtg aatttgatta atggcattgc cctagccttt gaaggagatg
    32341 cggtttattc ctactatgtt cgtcgtcatc tcatttttca aggtaaaacg aaacctagcc
    32401 agctacaccg tttagcaacg agatatgttt ctgctaaggc acaagccaac ttgattcagg
    32461 ctatgttaga agcgcagcta ttgaccgaaa aagaagaaga catctataag cgtggtcgca
    32521 ataccaatag ccatactaaa gctaagaatg ccgatattat tacctatcgt atgtcaacag
    32581 gttttgaagc cattatgggt tatcttgata tgatgggcca aaaagagcgg ttagaagaat
    32641 tgatcagatg gtgtattgag tatgtagaaa agcaacaatt gatatcctca taagagtccc
    32701 ctaggagact cttattttca taggtctgaa gtaaaggtag agagccatta actcttttta
    32761 tgacatcaaa aagacataat gatataaaaa agatacattt gtaacaaaaa agacataaaa
    32821 ataatgaaat cattttcgaa tagtttataa tgagagggta agactaattt aaagaggaga
    32881 tatttattat gacaactatg caaaaaacag ttagcttatt atcactagct ttacttattg
    32941 gtttgctggg gacttctggc aaagccatat ctgtgtatgc acaagatcag cacactgata
    33001 atgttatagc tgaatcaact attagtcagg tcagtgttga agccagtata cgtggaacag
    33061 aaccttatat tgatgctaca gtcaccacag atcaacctgt cagacaacca actcaggcaa
    33121 cgataacact taaagacgct agtgataata ctattaatag ttgggtatat actatggcag
    33181 cgcaacagcg tcgttttaca gcttggtttg atttaactgg acaaaagagt ggtgactatc
    33241 atgtaactgt caccgttcat actcaagaaa aggcagtaac tggtcaatca ggaactgttc
    33301 attttgatca aaacaaagct agaaaaacac caactaatat gcaacaaaag gatacttcta
    33361 aagcaacgac gaattcggtc gatgtagaca caaaagctca aacaaatcaa tcagctaacc
    33421 aagaaataga ttctacttca aatcctttca aatcagctac taatcatcga tcaacttcct
    33481 taaagcgatc tactaaaaat gagaaactta caccaactgc tagtaatagc caaaaaaacg
    33541 gtagcaacaa gacaaaaatg ctagtggaca aagaggaagt aaaacctact tcaaaaagag
    33601 gattcccttg ggtcttatca ggtctagtag tcagtttagc tgcaggttta tttatagcta
    33661 ttcaaaaagt atctagacga aaataaaaga gtctatgcat ttattttgaa gcattcatgg
    33721 tataataaac tcatggaaga taaagatact attgaaacaa acgatatcgt ctatggtgtt
    33781 catgccgtta cagaaagcct tcaagcaaat acaggaaata agctttatat ccaagaggat
    33841 ttaagaggaa agaaagtgga taacatcaaa agcttagcga cacaaaaaaa ggtcgctatt
    33901 tcatggacgc ctaaaaaaac cttgtcacaa atgactgatg gagctgtgca tcaaggtttt
    33961 gttctgagag tatcagcttt tgcctatact gatgttgatg agatcctcga aatagcagag
    34021 caagaagcaa atcctttgat tcttatttta gatggtctga cagatcctca taatttagga
    34081 tcgattttac ggacagctga tgctacaaat gtatgtggag tgattattcc taaacatcgt
    34141 tctgttggtg tgactccagt ggtctcaaag acgtctacag gtgctgttga acatattccg
    34201 atagcgagag taactaacct tagtcaaact ctagataaat tgaaagcaag aggattctgg
    34261 atttttggca cagacatgaa tggaacaccg tctgattgct ggaacactaa tggtaaactt
    34321 gctttagtca ttggtaacga aggcaaaggc atctctacca atatcaaaaa gcaagttgat
    34381 gaaatgatta cgattcctat gaatggtcac gtacagagct taaatgctag cgtagcggca
    34441 gctattctca tgtatgaagt tttccgaaat aggcgctaaa ttatgaaaaa acggatatta
    34501 ttagtagatg ggtataatat gattgccttt tggcaatcaa cccgtcagtt gtttaagata
    34561 aatcagcttg atcaggcacg taacacactt ttaacaaaac ttaatcatta tgcccatttt
    34621 gagaatatta atattatttg tgtttttgat gcccaatatg taccggggtt aaggcaacga
    34681 tatgaccagt attatatctc agtggtattt acagaggaag acgaaacagc agatagctac
    34741 attgagcgca tggcagcaga gttaaatacg gctatacata tggtagaagt agctacgagt
    34801 gatttaaatg aacaatggac gattttttct caaggagctc ttcgtgtcac tgcaagagaa
    34861 ttagagcaaa gagttcatac tgtcaaagct gatttagata aaatgtctag agatattgat
    34921 ctcaaaacgc ctaaactgcg gccttttgac caaggacagc ttattcaatt gaaggatttt
    34981 atgtctcagt tagatcgata atgagaatta cgactcgcca agtgcgggtt tttttgataa
    35041 aataaaacaa taattatttg attatcaaac taaaaaactg gatgaggaga agtaagaatg
    35101 acctttacaa taatgacaga ttcaaccgct gatttgaatc aaacctgggc agaagatcat
    35161 gatattgtcc ttataggatt aacgattttg tgcgatggag aggtttatga aacggtcggt
    35221 cctaaccgta ttagtagtga ttaccttttg aaaaagatga aagctggcag tcatcctcaa
    35281 accagtcaga ttaatgtggg agagtttgag aaggtttttc gtgaacatgc taggaacaac
    35341 aaagcactgc tttatcttgc tttttcttcg gttttatcgg gtacctatca aagcgctcta
    35401 atggcacgcg atcttgttcg agaagattat cccgatgcag tgattgaaat tgttgatacc
    35461 ttggctgctg caggaggaga aggctatcta accattttag cagcagaagc cagagatagt
    35521 ggcaaaaatt tactggagac taaagatatt gttgaagcag tgattcctcg actgcgcacc
    35581 tatttcttag tagatgatct ttttcatttg atgcgtggtg ggcgtctgtc aaaaggttca
    35641 gcttttctag gcagtttagc cagtatcaaa cctcttttgt ggattgatga ggaagggaaa
    35701 ttggtgccca ttgcaaaaat tcggggacgc caaaaagcca tcaaagaaat ggtggctcaa
    35761 gtggaaaaag atattgcgga ttcgacggtt attgtctctt atacaagcga tcagggtagt
    35821 gccgaaaagt tacgagaaga gctgctggcg catgagaata ttagcgatgt tcttatgatg
    35881 ccactaggac cagttatctc agctcacgtt ggtcctaaca ccctggcagt ttttgtgatt
    35941 gggcagaatt ctcgttaatc ccttataagg attgaagaga aatcctcaat ccttttttgc
    36001 tgcttaaatc aatcaagaat gtaaaatata tttgacaaaa tatccaaaat aatttatatt
    36061 atgtcatata aagatttaaa ggagaaacga atcatgtttg atagcaaaca aaatctcgct
    36121 aagtcacaat ggggtttcat tgtcataaca gcagtgatgg atctagttgt gatagtagca
    36181 acattactgg ctaagaccag ttttcaacgt tacttagttg gaggcgtagc ggttatattg
    36241 actagctttc tcatattgtt ggtatggggg ctaaagacag cgaaaaagct ccaaacgaga
    36301 ctagatagca aagaagtact agattctaag gtaagggacg atcagaagct gcccagatat
    36361 gacgaacgcc aaaaacagat tcttctcaaa ggatacacta ttgggttttg gtttatgatt
    36421 gtggctgttt ggctgtcaat ctttcttcat cgcttctcag agagactggt atctgcaagc
    36481 ttcctcttta cattggctct ttggggaggg ttggcagttc aaacgacata ttgtaacctc
    36541 aacggggtat cgccatttgt ggataggcgc tttggaaaac aaggcatact ggtggggatg
    36601 atagcaacag gaatgagttt aatcactata ggtttggcgg taagagagat gatggcaggg
    36661 caacttaaaa tgggaagctt tttcaaggct ggcggcagtg gatctttaat ggtgttgggc
    36721 ataacgctcc ttagtatggg agctagtatt ttctatcgtc attatctgga tgctaaagag
    36781 gcagatgaat gaagaacctc aaattaaaag cagcgcgtgc aggcaaagat ttgtctcaac
    36841 aagctctagc agatttggta ggcgtctcca gacaaaccat tgcggctgtt gaaaaaggtg
    36901 attacaaccc aacgattaat ctttgcattg ccatttgtcg agtacttgat aagaccttag
    36961 atgacctttt ttgggaggtt gatgattccc attaacatca gcagagcagc tctgccattc
    37021 ctcaaaaacg aaagaaaaaa taatccaata aaatcagcta aagcacttga ctggagcctc
    37081 ttaagtttga tatcatagta ggcggtattg tttaccccat ttgaaaggcc ccggaacctt
    37141 ccaaatacct tcgatgggat ggaacaccca tcaccttgta aacaaatatt acgaattcgt
    37201 ataggagaaa tcatgaacaa aacaactttc atggctaaac caggccaagt tgaacgcaaa
    37261 tggtacgttg ttgacgcaac tgatgtgcca cttggacgtc tttctgcagt agttgctagc
    37321 gtacttcgcg gaaaaaacaa accaactttc acaccacata ctgatacagg tgattttgtt
    37381 atcgttatca acgctgaaaa agttaaatta actggtaaaa aagcgactga taaagtttat
    37441 tacactcact caatgtaccc aggtggtttg aaatcaatca ctgctggtga acttcgctct
    37501 aaaaacgcag ttcgcttgat tgaaaaatca gttaaaggca tgcttccaca taacactctt
    37561 ggacgtgcac aaggtatgaa attgaaagtc ttcgttggcg gtgagcacac tcacgcagca
    37621 caacaaccag aagtacttga catctcagga cttatctaag aggaaaggag cattaaataa
    37681 tggcacaagc acaatatgca ggtactggcc gtcgtaaaaa cgctgttgca cgcgttcgtt
    37741 tggttccagg tactggtaaa atcactgtta acaaaaaaga tgtagaagaa tacatcccac
    37801 atgctgacct acgtttgatc attaaccaac cttttgcagt tacatcaact gaaggttcat
    37861 acgacgtttt cgttaacgtt gttggtggtg gttacggtgg acaatcaggt gcgatccgtc
    37921 atggtatcgc tcgtgcgctt cttcaagtag acccagactt ccgcgattcg ttgaaacgcg
    37981 ctggccttct tacacgtgac gcacgtatgg ttgaacgtaa aaaaccaggt cttaagaaag
    38041 ctcgtaaagc ttcacaattc tcaaaacgtt aagaacagct ttacatcaac gattacaagc
    38101 attttgcaag tctcttgcaa ggtgcttttt ttgtcgtttt ttgcttttga tacctttttt
    38161 tgataccgtt tcggaaaact tttgacttgg tttaatacgt ttatttagtt atttttgaga
    38221 tagttagcaa attgtttgat tgatttgtct tctccgactg cagtagcatg actgtatgta
    38281 tcaagagtta tttgactact agcatgtcct aaccttttgg ctgtatttac tgggtcaact
    38341 ccaatctcaa tcatcagggt tgcatgggta tgcctgaagc cgtgaggact tattttttaa
    38401 gactatgttg cttaatgacc tttgataaga tattgttaat atagtctgta tgaagtggtt
    38461 caatactccc ttctctagta cagtatgtaa atataaagtc agactcaatt gaaagaggtg
    38521 caactgctag ttgtgccttt tctttttggc tgatggcttt ccattcttgc agaatagaaa
    38581 tagtttcgct gtctagctta atctttctta ctgagaaatc atttttagtg cttttttcga
    38641 ttgcctgtcc gtctaatcgt cctaggctct tattgacgct tagtgtctca ctctgaaagt
    38701 ctatattagc ccatttaaga gcgcatagct cgcctttcct taaggccgct ataagctagt
    38761 agccgaaata gtgcgtagtg cttgtaaggc tctttctgaa gtactatggc taaaaactcc
    38821 tgaagttctt gaaccgtcca atagttttct attcgtgtct ttttccgctt tggcataatg
    38881 atttcagcca tagggttatg cttgagcagt ttcatcttga tggcaaaatc aaagatcttt
    38941 tcagtgtagg acttgatttg cttgatattc ttaaaggtct ttgctttatc ggtgatgaag
    39001 ttctggcagt ctaaagggga ggttttgtta atgggcaatt ctcccatgac tggcaaaatg
    39061 tgaagttgaa acagatcaag agtacgagaa gccgtggttg gctctaccgt atcttgatag
    39121 gcttgatacc attcctcgaa tatctcgcta taagttttat gagttggctt ggtgaactcc
    39181 tctaagtttc ctgaagcctt aaggtagatt agcctatctt cgtagtcttt ggcttctttg
    39241 tggaatatct actaaacagc ccgaacttat tttgctccta gttaattggt aatctaccaa
    39301 aaaagcccta gcaagactgc taaggctttt aatgtgttat ttgaataact ttatatagtc
    39361 gctttgagta ggaagcaagt aatcaataac gaccctattt tcttccccct cgatatggta
    39421 taggacaata taatctttac ttaaggtata acctttagtg ctgtgataat ggatgatttt
    39481 tgaaccatat ttttcatcgg catcaaaacc aacttctcgg aaaacctcga gtttttctat
    39541 gtcgctaatg atttgctcca ttttacgctg acctgatgtt gaggaatagt tttgagaaat
    39601 ataatcacga atttctttta gcttctctaa aacatcagga gcatagataa tctgatattt
    39661 cttatagtcc aagttcagcc ctcacttctt cagaggtata aaacttccct tgacgaacat
    39721 cctcaatatt tttgttgatt tcagcttgaa attgcttgaa aagtttttct ttctctaact
    39781 cctcttccgt caataagtca acctcgtttg tgacaacgac attttgtagg aataatctta
    39841 aagcagatga taaggttaaa ttttttttgt ttaatactgt cattgcttcg cttaccaatt
    39901 ctttattagc ttgaaaggta accgctctgt tttttttact gtagtcattt ta
//
LOCUS       DATXIG010000013        36822 bp    DNA     linear   BCT 28-DEC-2023
DEFINITION  TPA_asm: Streptococcus pyogenes strain MGAS11113
            SAMN08555799-rid19682583.denovo.013, whole genome shotgun sequence.
ACCESSION   DATXIG010000013 DATXIG010000000
VERSION     DATXIG010000013.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN08555799
            Sequence Read Archive: SRR6740495
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pyogenes
  ORGANISM  Streptococcus pyogenes
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 36822)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 36822)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-OCT-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-OCT-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001918058.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001918058.2
            Genome Coverage       :: 368x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/12/2023 11:23:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 1,806
            CDSs (total)                      :: 1,769
            Genes (coding)                    :: 1,722
            CDSs (with protein)               :: 1,722
            Genes (RNA)                       :: 37
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1, 1 (5S, 23S)
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 47
            CDSs (without protein)            :: 47
            Pseudo Genes (ambiguous residues) :: 0 of 47
            Pseudo Genes (frameshifted)       :: 30 of 47
            Pseudo Genes (incomplete)         :: 20 of 47
            Pseudo Genes (internal stop)      :: 9 of 47
            Pseudo Genes (multiple problems)  :: 11 of 47
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..36822
                     /organism="Streptococcus pyogenes"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN08555799-rid19682583.denovo.013"
                     /strain="MGAS11113"
                     /serotype="Emm28"
                     /isolation_source="normally sterile site"
                     /host="Homo sapiens"
                     /db_xref="taxon:1314"
                     /country="Finland"
                     /collection_date="2002"
                     /collected_by="National Public Health Laboratory"
     gene            complement(148..1452)
                     /locus_tag="VC122_000663"
     CDS             complement(148..1452)
                     /locus_tag="VC122_000663"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011527521.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FtsW/RodA/SpoVE family cell cycle protein"
                     /protein_id="HEP5640607.1"
                     /translation="MIISRSRGKTMKIDKRHLLNYSILLPYLILSVIGLIMVYSTTSV
                     SLIQAHANPFKSVINQGVFWIISLVAITFIYKLKLNFLTNTRVLTVVMLGEAFLLIIA
                     RFFTTAIKGAHGWIVIGPVSFQPAEYLKIIMVWYLALTFAKIQKNISLYDYQALTRRK
                     WWPTQWNDLRDWRVYSLLMVLLVAAQPDLGNASIIVLTAIIMFSISGIGYRWFSAILV
                     MITGLSTVFLGTIAVIGVERVAKIPVFGYVAKRFSAFFNPFHDLTDSGHQLANSYYAM
                     SNGGWFGQGLGNSIEKRGYLPEAQTDFVFSVVIEELGLIGAGFILALVFFLILRIMNV
                     GIKAKNPFNAMMALGVGGMMLMQVFVNIGGISGLIPSTGVTFPFLSQGGNSLLVLSVA
                     VGFVLNIDASEKRDDIFKEAELSYRKDTRKENSKVVNIKQFQ"
     gene            complement(1592..4390)
                     /gene="ppc"
                     /locus_tag="VC122_000664"
     CDS             complement(1592..4390)
                     /gene="ppc"
                     /locus_tag="VC122_000664"
                     /EC_number="4.1.1.31"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002985527.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoenolpyruvate carboxylase"
                     /protein_id="HEP5640608.1"
                     /translation="MIEETSKEGDGHLPLKKLESSNNQAIIAEEVALLKEMLENITRR
                     MIGDDAFAVIESIMVLSEKQDYIELEKVVANISNQEMEVISRYFSILPLLINISEDVD
                     LAYEINYQNNTDTDYLGKLALTIKDLAGKDNGKDILEQVNVVPVLTAHPTQVQRKTIL
                     ELTTHIHKLLRKYRDAKAGVINLEKWRQELYRYIEMIMQTDIIREKKLQVKNEIKNVM
                     QYYDGSLIQAVTKLTTEYKNLAQKHGLELDNPKPITMGMWIGGDRDGNPFVTAETLCL
                     SATVQSEVILNYYIDKLAALYRTFSLSSTLVQPNSEVERLASLSQDQSIYRGNEPYRR
                     AFHYIQSRLKQTQIQLTNQPAARMSSSVGLSTSAWSSPASLENPILAYDSPVDFKADL
                     KAIEQSLLDNGNSALIEGDLREVMQAVDIFGFFLASIDMRQDSSVQESCVAELLKGAN
                     IVDDYSSLSETEKCDVLLQQLMEEPRTLSSAAVAKSDLLEKELAIYTTARELKDKLGE
                     EVIKQHIISHTESVSDMFELAIMLKEVGLVDQQRARVQIVPLFETIEDLDNARDIMAA
                     YLSHDIVKSWIATNRNYQEIMLGYSDSNKDGGYLASGWTLYKAQNELTAIGEEHGVKI
                     TFFHGRGGTVGRGGGPSYDAITSQPFGSIKDRIRLTEQGEIIENKYGNKDVAYYHLEM
                     LISASINRMVTQMITDPNEIDSFREIMDSIVADSNTIYRKLVFDNPHFYDYFFEASPI
                     KEVSSLNIGSRPAARKTITEITGLRAIPWVFSWSQNRIMFPGWYGVGSAFKRYIDRAQ
                     GNLERLQHMYQTWPFFHSLLSNVDMVLSKSNMNIAFQYAQLAESQDVRDVFYEILDEW
                     QLTKNVILAIQDHDDLLEDNPSLKHSLKSRLPYFNVLNYIQIELIKRWRNNQLDENDE
                     KLIHTTINGIATGLRNSG"
     gene            4549..6348
                     /gene="pepF"
                     /locus_tag="VC122_000665"
     CDS             4549..6348
                     /gene="pepF"
                     /locus_tag="VC122_000665"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012766712.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oligoendopeptidase F"
                     /protein_id="HEP5640609.1"
                     /translation="MELKKRSEFPENELWDLTALYKDRQDFLLAIEKALQDIDLFKRN
                     YEGRLTSVDDFTQALIEIEHIYIQMSHIGTYAFMPQTTDFSDESFAQIAQAGDDFMTK
                     ASVALSFFDTALANADLDVLDTLEKNPYFSAAIRMAKIQKEHLLSPDVEKALANLREV
                     INAPYDIYTKMRAGDFDMEDFEVDGKTYKNSFVSYENFYQNHENAEIRDKAFRSFSKG
                     LRKHQNTAAAAYLAKVKSEKLLADMKGYASVFDYLLAEQEVDRSLFDRQIDLIMTEFG
                     PVAQKFLKHVAQVNGLEKMTFADWKLDIDNDLNPEVSIDGAYDLVMKSLAPLGQEYTK
                     EIERYQTERWVDFAANANKDSGGYAADPYKVHPYVLMSWTGRMSDVYTLIHEIGHSGQ
                     FIFSDNHQSYFNTHMSTYYVEAPSTFNELMLSDYLEHQFDDPRQKRFALAHRLTDTYF
                     HNFITHLLEAAFQRKVYTLIEEGGTFGADQLNAMMKEVLTDFWGDAVDIDDDAALTWM
                     RQAHYYMGLYSYTYSAGLVISTAGYLNLKNNPDGAKEWLDFLKSGGSRTPLDTAMLIG
                     ADIATEKPLRDTIQFLSDTVDQIINYTEEMSHA"
     gene            6341..6820
                     /locus_tag="VC122_000666"
     CDS             6341..6820
                     /locus_tag="VC122_000666"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003054718.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione peroxidase"
                     /protein_id="HEP5640610.1"
                     /translation="MPNLYDFTVKAQNGNDLSLAAYKKKVVLIVNTATKCGLTPQYQA
                     LQALYDTYHDKGFEVLDFPCNQFLNQAPGDAEEINHFCSLTYHTTFPRFAKIKVNGKD
                     ADPLFTWLKEEKSGPLGKRIEWNFTKFLIDQNGQVIKRYSSKTDPKLIEEDLKALLG"
     gene            complement(6867..7250)
                     /locus_tag="VC122_000667"
     CDS             complement(6867..7250)
                     /locus_tag="VC122_000667"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002990551.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4430 domain-containing protein"
                     /protein_id="HEP5640611.1"
                     /translation="MNKRFKTGFLALVAMLLVACSQGTKQTQTPSVPKADHHVRLVVK
                     EDTNTIDEKVSFGKGDTVLEILKDNYEVKEKDGFITAIDGIEQDTKANKYWLFKVNGK
                     MADKGADQITVKDGDSIEFYQEVFK"
     gene            complement(7234..7737)
                     /locus_tag="VC122_000668"
     CDS             complement(7234..7737)
                     /locus_tag="VC122_000668"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011284619.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ECF transporter S component"
                     /protein_id="HEP5640612.1"
                     /translation="MSAKFMSRIAIMSALCVVLRMVFSSLPNVQPVTAFLLSYLLYFG
                     LAEAVLVMMLCLFLSAFLLGFGPWVFWQVTCFVLVLLLWRFALYPLSQQFPKYQLGYQ
                     AFLAALCGLLYGVLIDTCFAYLYSMPWWSYVLAGMPFNIAHALSTLVFFPVVMMLFRR
                     LIGEQKI"
     gene            8062..8910
                     /locus_tag="VC122_000669"
     CDS             8062..8910
                     /locus_tag="VC122_000669"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002985536.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 25 protein"
                     /protein_id="HEP5640613.1"
                     /translation="MRRKIKPIVVLVFFILLAMVLIIGKRQANHAKQKEVEDAKSHIP
                     IATSNPSKAKTSTSETEDFILNPIVDVSGWQLPEEIDYDTLSRHISGAIVRVYGGSQI
                     TAHNNAAFTTGIDKSFKTHIKEFQKRNVPVAVYSYALGRSTKEMKEEARAFYKNAAPY
                     NPTYYWIDVEEATMKDMNKGVTAFREELKKLGAENVGLYIGTYFMAEQDISTKGFDAV
                     WIPTYGSDSGYYEAAPNTTLDYDLHQYTSQGYLSGFNNALDLNQIAVTKDTKKTFEKL
                     FGNSNN"
     regulatory      9038..9134
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00059"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="TPP riboswitch; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="thiamine pyrophosphate"
                     /db_xref="RFAM:RF00059"
     gene            9179..9742
                     /gene="thiT"
                     /locus_tag="VC122_000670"
     CDS             9179..9742
                     /gene="thiT"
                     /locus_tag="VC122_000670"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002990555.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="energy-coupled thiamine transporter ThiT"
                     /protein_id="HEP5640614.1"
                     /translation="MSPNTNVKYLIEAAIFAALAMALSFIPDFAGWFSPSYGAIALVI
                     FSLRRGLKYGMLAGLIWGLLHFVLGKVYYLSMSQVFIEYILAFTSMGLAGSFSDSLIK
                     TLRRQQTFFAVFLAIMASLLAVTVRYLWHFLAGIIFWGSYAPKGMSAVWYSFSVNGTA
                     GVLTFLITCLALMIALPIHPQLFDPKD"
     gene            9832..10311
                     /locus_tag="VC122_000671"
     CDS             9832..10311
                     /locus_tag="VC122_000671"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002990557.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminoacyl-tRNA deacylase"
                     /protein_id="HEP5640615.1"
                     /translation="MAKKTKFKKTLVEQILDKANIAHQGLKLNALEGDFPDDLQPSDI
                     YKTLALTGDQTGPLIGIIPLTEHLSEKQLAKVSGNKKVSMVPQKDLQKTTGYIHGANN
                     PVGIRQKHSYPIFIDQTALEKGQIIVSAGEVGRSIKISSQALADFVGASFADLKKRK"
     gene            10652..11275
                     /locus_tag="VC122_000672"
     CDS             10652..11275
                     /locus_tag="VC122_000672"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011889060.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine phosphatase family protein"
                     /protein_id="HEP5640616.1"
                     /translation="MKLYFVRHGKTLWNLEGRFQGAGGDSPLLEEAKDEIHLLGKELA
                     KVAFDAVYTSDLQRAMATAAIILDAFDQQPKLYHTDQLREWRLGKLEGAKIATMAAIY
                     PQQMLAFRENLAQFKSDQFEAESIYQTTQRVCHLIQSFKDKHYQNVLIVGHGANLTAT
                     IRSLLGFEPALLLAKGGLDNTSLTILETKDYLTYDCLIWNDKSFLEV"
     gene            complement(11383..12285)
                     /locus_tag="VC122_000673"
     CDS             complement(11383..12285)
                     /locus_tag="VC122_000673"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000633099.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family hydrolase"
                     /protein_id="HEP5640617.1"
                     /translation="MITAIVFDVDDTIYDQQAPYRIAMEKCFPDFDMSVMNQAYIRFR
                     HYSDVGFPRVMAGEWTTEYFRFWRCKETLLEFGYREIDEAAGVHFQEVYEHELENITM
                     LDEMRMTLDFLKSKNVPMGIITNGPTEHQLKKVRKLGLYDYIDPKRVIVSQATGFQKP
                     EKEIFNLAAEQFDMNPQTTLYVGDSYDNDIMGAFNGGWHSMWFNHRGRQLKPGTKPVY
                     DVAIDNFEQLFGAVKVLFDLPDNKFIFDVNDKKNPILQMGINNGLMMAAERLLESNMS
                     IDKVVILLRLTKQQEKVLRLKYAR"
     gene            12459..13952
                     /gene="lysS"
                     /locus_tag="VC122_000674"
     CDS             12459..13952
                     /gene="lysS"
                     /locus_tag="VC122_000674"
                     /EC_number="6.1.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014411828.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysine--tRNA ligase"
                     /protein_id="HEP5640618.1"
                     /translation="MSNQHIEELNDQQIVRREKMTALAEQGIDPFGKRFDRTANSAEL
                     KEKYADKTKEELHELNETAIVAGRLMTKRGKGKVGFAHLQDREGQIQLYVRKDSVGED
                     NYEIFKKADLGDFIGVEGEVMRTDMGELSIKATKLTHLSKSLRPLPEKFHGLTDIETI
                     YRKRHLDLISNRESFDRFVTRSKMISEIRRYLDGLDFLEVETPVLHNEAGGAAARPFV
                     THHNAQNIDMVLRIATELHLKRLIVGGMERVYEIGRIFRNEGMDATHNPEFTSIEVYQ
                     AYADYLDIMNLTEGIIQHAAKAVKGDGPIDYQGTEIRINEPFKRVHMVDAIKEVTGVD
                     FWPEMTVEEAIALAKEKQVPLEKHFTSVGHIINAFFEEFVEETLVQPTFVFGHPVEVS
                     PLAKKNPEDTRFTDRFELFIMTKEYANAFTELNDPIDQLSRFEAQAQAKELGDDEATG
                     IDYDFVEALEYGMPPTGGLGIGIDRLCMLLTNTTTIRDVLLFPTMKP"
     gene            14088..14228
                     /locus_tag="VC122_000675"
     CDS             14088..14228
                     /locus_tag="VC122_000675"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011284615.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEP5640619.1"
                     /translation="MTKEYISYISAAIFLLYGLLAAKWIFLGLGLILVLVGLADRLNS
                     KK"
     gene            complement(14325..14537)
                     /locus_tag="VC122_000676"
     CDS             complement(14325..14537)
                     /locus_tag="VC122_000676"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003049995.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdbC family protein"
                     /protein_id="HEP5640620.1"
                     /translation="MAEFTFNIEEHLLTLSENDKGWTKELNRVSFNGAEAKWDIRTWS
                     PDHTKMGKGITLTNEEFKTILDAFRK"
     gene            complement(14754..16040)
                     /locus_tag="VC122_000677"
     CDS             complement(14754..16040)
                     /locus_tag="VC122_000677"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002994498.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="U32 family peptidase"
                     /protein_id="HEP5640621.1"
                     /translation="MSHMKKRPEVLSPAGTLEKLKVAIDYGADAVFVGGQAYGLRSRA
                     GNFSMEELQEGIDYAHARGAKVYVAANMVTHEGNEIGAGEWFRQLRDMGLDAVIVSDP
                     ALIIICSTEAPGLEIHLSTQASSTNYETFEFWKAMGLTRVVLAREVNMAELAEIRKRT
                     DVEIEAFVHGAMCISYSGRCVLSNHMSHRDANRGGCSQSCRWKYDLYDMPFGGERRSL
                     KGEIPEDYSMSSVDMCMIDHIPDLIENGVDSLKIEGRMKSIHYVSTVTNCYKAAVDAY
                     MESPEAFYAIKEELIDELWKVAQRELATGFYYGIPTENEQLFGARRKIPQYKFVGEVV
                     AFDSASMTATIRQRNVIMEGDRIECYGPGFRHFETVVKDLHDADGQKIDRAPNPMELL
                     TISLPREVKPGDMIRACKEGLVNLYQKDGTSKTVRT"
     gene            complement(16142..17068)
                     /locus_tag="VC122_000678"
     CDS             complement(16142..17068)
                     /locus_tag="VC122_000678"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015016802.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="U32 family peptidase"
                     /protein_id="HEP5640622.1"
                     /translation="MEKIIITATAESIEQVKALLAAGVDRIYVGEANYGLRLPHNFSY
                     DELRQIAKLVHDAGKELTVACNALMHQDMMDQIKPFLDLMIEIAVDYLVVGDAGVFYV
                     NKRDGYNFKLIYDTSVFVTSSRQVNFWGQHGAVESVLAREIPSAELFTLAENLEFPAE
                     VLVYGASVIHHSKRPLLENYYHFTKIDDEVSRERGLFLAEPGDASSHYSIYEDNHGTH
                     IFINNDIDMMSKLGELYAHGLTHWKLDGIYCPGDDFVAITKLFIQAKTLLEAGQFTQE
                     EAEKLDQAVHAHHPAGRGLDTGFYEFDPKTVK"
     gene            17267..17551
                     /locus_tag="VC122_000679"
     CDS             17267..17551
                     /locus_tag="VC122_000679"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002990571.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3270 domain-containing protein"
                     /protein_id="HEP5640623.1"
                     /translation="MPAPLKKHNSVEQDDSYQESQSKFRDFQEFNGQSAKLKELVFFA
                     RIASFSLITVLFAFVLLVMNLAPMFAFLFASIISLGITSLFAKVIKSLLM"
     gene            complement(17711..18139)
                     /locus_tag="VC122_000680"
     CDS             complement(17711..18139)
                     /locus_tag="VC122_000680"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002994493.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YtxH domain-containing protein"
                     /protein_id="HEP5640624.1"
                     /translation="MNKSFKNLVIGAVSGVAAAYFLSTEKGKALKNRAEKAYQAYKES
                     PDDYHQFAKEKGSEYSHLARDTFYDVKDKLASGDLTKEDMLDLLKDKTTAFVQKTKET
                     LAEVEAKEKQDDVIIDLNEEDIIIDYTEQDEPVSDTLDKH"
     gene            complement(18132..18539)
                     /locus_tag="VC122_000681"
     CDS             complement(18132..18539)
                     /locus_tag="VC122_000681"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014635358.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF948 domain-containing protein"
                     /protein_id="HEP5640625.1"
                     /translation="MDLVGISLMIIALAFVALVIFLIIVLKKVSETIDEAKKTISVLT
                     SDVNVTLHQTNDILAKANILVEDVNGKVATIDPLFVAIADLSESLSDLNSQARHFGQK
                     ATNATGNVSKAGKLALVGKVASKVFGKKGEKHE"
     gene            complement(18561..19340)
                     /locus_tag="VC122_000682"
     CDS             complement(18561..19340)
                     /locus_tag="VC122_000682"
                     /EC_number="2.4.99.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011106849.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prolipoprotein diacylglyceryl transferase"
                     /protein_id="HEP5640626.1"
                     /translation="MINPIALKCGPLAIHWYALCILSGLVLAVYLASKEAPKKGISSD
                     AIFDFILIAFPLAIVGARIYYVIFEWSYYVKHLDEIIAIWNGGIAIYGGLITGALVLL
                     AYCYNKVLNPIHFLDIAAPSVMVAQAIGRWGNFINQEAYGKAVSQLNYLPSFIQKQMF
                     IEGSYRIPTFLYESLWNLLGFVIIMMWRRKPKSLLDGEIFAFYLIWYGSGRLVIEGMR
                     TDSLMFLGIRISQYVSALLIIIGLIFVIKRRRQKGISYYQE"
     gene            complement(19337..20269)
                     /locus_tag="VC122_000683"
     CDS             complement(19337..20269)
                     /locus_tag="VC122_000683"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012515957.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HPr kinase/phosphorylase"
                     /protein_id="HEP5640627.1"
                     /translation="MTVTVKMLVQKVKLDVVYATDNLLSKEITTSDISRPGLEMTGYF
                     DYYAPERLQLFGMKEWSYLTQMTSHNRYSVLKEMFKKDTPAVVVSRNLAIPKEMVQAA
                     KEEGISLLSSRVSTSRLAGEMSYFLDASLAERTSVHGVLMDIYGMGVLIQGDSGIGKS
                     ETGLELVKRGHRLVADDRVDVYAKDEETLWGEPAEILRHLLEIRGVGIIDVMSLYGAS
                     AVKDSSQVQLAIYLENFEAGKVFDRLGNGNEEITFSGVRIPRIRIPVKTGRNVSVVIE
                     AAAMNHRAKEMGFDATKTFEDRLTQLITKNEVSQ"
     gene            complement(20634..20906)
                     /locus_tag="VC122_000684"
     CDS             complement(20634..20906)
                     /locus_tag="VC122_000684"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003059402.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PspC domain-containing protein"
                     /protein_id="HEP5640628.1"
                     /translation="METKFYKQRKNRLVAGVIAGLADKYGWDLALARVLAALLIYGTG
                     FGVLLYILLAIFLPYKEDLLEERYGRGPRRRKDADVLNEEEDGWFW"
     gene            complement(20970..21407)
                     /locus_tag="VC122_000685"
     CDS             complement(20970..21407)
                     /locus_tag="VC122_000685"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014407418.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SprT family protein"
                     /protein_id="HEP5640629.1"
                     /translation="MTLTNYVQEVSLADFGKPFHHKAYWNKRLKTTGGRFFPKDGHLD
                     FNPRMLEEHGELIFRKIVRHELCHYHLYFEGRGYHHKDRDFKDLLAQVNGLRYVPTSS
                     KSKTNHHYSCQTCGQVYQRKRRINLAKYVCGNCHGKLMEKNQS"
     gene            complement(21394..23526)
                     /locus_tag="VC122_000686"
     CDS             complement(21394..23526)
                     /locus_tag="VC122_000686"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003049981.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA-binding transcriptional accessory protein"
                     /protein_id="HEP5640630.1"
                     /translation="MENNNNHNIAEALSVSLHQIEQVLALTAQGNTIPFIARYRKEVT
                     GNLDEVVIKSIIDMDKSLTTLNERKATILAKIEEQGKLTDQLRTSIEATEKLADLEEL
                     YLPYKEKRRTKATIAREAGLFPLARLILQNAQNLETAAEPFVTEGFASPQEALAGAVD
                     ILVEAMSEDAKLRSWTYNEIWQYSRLVSTLKDEQLDEKKVFQIYYDFSDQVSNMQGYR
                     TLALNRGEKLGILKVSFEHNLEKMQRFFSVRFKETNPYIEEVINQTIKKKIVPAMERR
                     VRSELSDAAEDGAIHLFSENLRHLLLVSPLKGKMVLGFDPAFRTGAKLAIVDQTGKLL
                     TTQVIYPVAPASQTKIQAAKETLTQLIETYQIDIIAIGNGTASRESEAFVADVLKDFP
                     NTSYVIVNESGASVYSASELARHEFPDLTVEKRSAISIARRLQDPLAELVKIDPKSIG
                     VGQYQHDVSQKKLSENLGFVVDTVVNQVGVNVNTASPSLLAHVSGLNKTISENIVKYR
                     EENGALTSRADIKKVPRLGAKAFEQAAGFLRIPGAKNILDNTGVHPESYPAVKELFKV
                     LGIQDLDDAAKATLAAVQVPQMAETLAIGQETLKDIIADLLKPGRDLRDDFEAPILRQ
                     DILDLKDLEIGQKLEGTVRNVVDFGAFVDIGVHEDGLIHISEMSKTFVNHPSQVVSVG
                     DLVTVWVSKIDLDRHKVNLSLLPPRDTH"
     gene            23760..23915
                     /locus_tag="VC122_000687"
     CDS             23760..23915
                     /locus_tag="VC122_000687"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002985576.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEP5640631.1"
                     /translation="MAITHKKNDELEKMLAGFASIPSFDKPLEVNTDGKLATKEKTTE
                     QTKPSKP"
     gene            24063..24965
                     /locus_tag="VC122_000688"
     CDS             24063..24965
                     /locus_tag="VC122_000688"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016480616.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="permease"
                     /protein_id="HEP5640632.1"
                     /translation="MSLFSNLPPSVLQWFAIFMSIIIEALPFVLLGTILSGCIEVFVT
                     PELVQKYLPKQKCLRILFGTFVGFVFPSCECGIIPIINRFLEKKVPSYTAVPFLATAP
                     IINPIVLFATYSAFGNSLRFLILRLVGAALVAITLGVMLAFIVDDNILKDNAQPVHFH
                     DYSHESLPKRIYLALVHAIDEFFDTGRYLVFGTLIASAMQIYVPTRVLTTIGHNPLTA
                     ILIMMLMAFILSLCSEADAFIGASLLSTFGVAPVLAFLLIGPMVDIKNLMMMVKTFKG
                     RFIVQFIGVSVLMIAVYCLLVGVL"
     gene            24965..25780
                     /locus_tag="VC122_000689"
     CDS             24965..25780
                     /locus_tag="VC122_000689"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002991931.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR03943 family protein"
                     /protein_id="HEP5640633.1"
                     /translation="MIRFLILAGYFELTMYLQLSGKLDQYINVRYSYLAYISMVLSFI
                     LALVQLYTWMKNIKVHSHLTGKIARLTSPFILVFPVLIGLLVPTVTLDSTTVSAKGYT
                     FPLAAGASKTGVSDDGTTIQYLKPDTSLYFTKSAYQKEMRQELHKYKGKKPVTITTEN
                     YMEVMELIYLYPDEFLDRDIQYTGFVYNEPGHDNYQFLFRFGIIHCIADSGVYGLLTT
                     GNQTSYPNNTWLTVKGRLHMEYDKNLEQHLPVLQLTEVHQTKEPNNPYVYRVF"
     gene            complement(25879..27303)
                     /locus_tag="VC122_000690"
     CDS             complement(25879..27303)
                     /locus_tag="VC122_000690"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012766692.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 1 protein"
                     /protein_id="HEP5640634.1"
                     /translation="MGIFPKDFLWGGAVAANQVEGAFEADTKGLSVQDVLPNGGLGEW
                     TDSPTSDNLTLEAIDFYHRYKEDIALFAEMDFKVFRTSIAWSRIFPNGDDDQPNEAGL
                     QFYDDLFDELLNYGIEPLVTLSHYETPLHLAKAYNGWTDRRLIGFFERFAQTVMERYK
                     DKVKYWLTFNEVNSILHMPFTSGGIMTEKEKLSLQDLYQAIHHELVASASVTKLAHEI
                     NPDVKVGCMILAMPAYPMTSDPRDILAAHAFENLNLLFSDIHVRGKYPSYIKSYFKEN
                     GIEIVFEDGDKELLAEHTVDFLSFSYYMSVTQAHNPEAYTSGQGNILGGLSNPYLESS
                     EWGWQIDPIGLRLVLNQYYDRYQIPLFIVENGLGAKDQLVQTADGSMTVHDDYRIDYM
                     SQHLVQVAKAIEDGVEVMGYTAWGCIDCVSMSTAQLSKRYGFIYVDRNDDGTGQLTRY
                     KKKSFDWYRQVIQTNGRYLEDNFS"
     gene            complement(27322..29184)
                     /locus_tag="VC122_000691"
     CDS             complement(27322..29184)
                     /locus_tag="VC122_000691"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003047292.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS beta-glucoside transporter subunit IIBCA"
                     /protein_id="HEP5640635.1"
                     /translation="MTYQETAKAILAAVGGKTNIQRVTHCVTRLRLVLKNDEKVKDQQ
                     VKAISNVIGVMRKNGQYQIILGNDVNNYYQAFLSLGHFDNQDEDHSSKAKGSILERLI
                     ETIAGVITPLIPALLGGGMLKVVGILLPMLGLASADSQTVAFINFFGDAAYYFMPVMI
                     AYSAAARFKVTPVLAATIAGILLHPAFVAMVAEGKPLTLFGAPVTPASYGSSVIPILM
                     MVYLMQYIEKWVNRLVPSVMKSFLQPTLIILISGFLALVVVGPLGVIIGQGLSNTMLA
                     IYHVAPWLALAILGAIMPLVVMTGMHWAFAPIFLAASVATPDVLILPAMLASNLAQGA
                     ASLAVAFKTKQKQTRQVALAAGISALLAGITEPALYGVTLKFKKPLYAAMISGGLVGA
                     FIGFVNIASYTFVVPSIIGLPQYINPSGGANFINALIAGAATIVLAFSLTWFMGIDEE
                     SPKQVSVAADMSQVKSGLSTKQTLYAPMTGEMLFLSEVPDETFSSKLLGEGFAILPSE
                     GVVYAPFDGEVITFFPTKHAVALKNTRGVEVLIHVGIDTVELKGQGFEQLVSVGDVVK
                     RGQALLKMDIDFITSKGYSLISPVVVTNSAEQLEIIIQDDKKMVTKEDALLVIL"
     gene            complement(29186..30028)
                     /locus_tag="VC122_000692"
     CDS             complement(29186..30028)
                     /locus_tag="VC122_000692"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011017522.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PRD domain-containing protein"
                     /protein_id="HEP5640636.1"
                     /translation="MLIKRVLNHNAAISTNHQGLDILLMGKGIAFGKKVGDSIELNAI
                     ETSFVLKNSDNMNRFTELFITVPQEVVACSERIINLGKIKLGKTLDEILYINLTDHIH
                     SAIERHEQGMLIHNPLRWEIQRYYPDEYSLGVKALELIERNLGVTLAIDEAAFIAMHF
                     VNASLDTPFKEPHRLTEIVSYIEQKIKTDFKTELDDTSIDYYRFMTHIKLFAQRVLSE
                     MSYDDDDAELLLVVKTKYPKEYRCVLDISEEIKKRYNYHLNSSELLYLTVHVKRLVKH
                     LKET"
     gene            30356..31723
                     /locus_tag="VC122_000693"
     CDS             30356..31723
                     /locus_tag="VC122_000693"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010922014.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HEP5640637.1"
                     /translation="MSHHQQTVSKQTIMAIIAIALIGFSGILSETSMNVTFPTLMSVY
                     QLPLNSLQWMTTIYLLAVAIMMTTSATLKKNVRERPLFFMATGLFTFGTILAVLTQSF
                     AIMLLARIFQGIGTGLVMPQMFNIILERVPMHKVGLFMGFAGLIISLAPAFGPTYGGF
                     MISHFSWQWIFICILPVPLIAGILAYYYLEDSPVSEKVPFDWLAFIALSISLTSALLA
                     ITSLENGSVNLYYLGLFILSFVLFLYKNLTAKQPFLDIRILKIPSLTFGLIPFFVFQL
                     INLGINFLTPNFIVMEKIANSSQAGMVLLPGTLLGALLAPAFGKLYDQKGAKLSLYLG
                     NALFSLSLIIMTLQTRHFMLLPFTLLYILFTFGRNMGFNNSLATAIRELPAEKNADAT
                     AIFQMMQQFAGALGTAMASLIANSQPEFTSGVQSVYLLFTIFALLDFIFFFAMFYHLG
                     KKGLA"
     gene            complement(31777..33327)
                     /gene="ftsY"
                     /locus_tag="VC122_000694"
     CDS             complement(31777..33327)
                     /gene="ftsY"
                     /locus_tag="VC122_000694"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002985588.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="signal recognition particle-docking protein
                     FtsY"
                     /protein_id="HEP5640638.1"
                     /translation="MGLFDRLFGKKETPKVAEEKLEENLLTETTQKEELSEKANEQNK
                     IEVVQQEDVSSEGAGSVENGPEAASVNAQVEEETGDNSNHPSEDTNEFAADKTDLKVS
                     ELSQSTASEPKDLVDQPVVEQFPTKQAQADASNDSANEEAVDTSKEQSSSQQVMEDYY
                     RRKAALEKSLQEKAAATVPVMPEEVPQENQASTSAEASQNKATHDTIPETDQEKYKRS
                     LKKTRTGFSARLNSFFANFRRVDEEFFEDLEEMLILSDVGVHVATTLTEELRYEAKLE
                     NAKKPDALKRVIVEKLVDIYEKDGRYNEAINYQDGLTVMLFVGVNGVGKTTSIGKLAY
                     RYKQEGKKVMLVAADTFRAGAVAQLVEWGRRVDVPVITGPEKADPASVVFDGMEKAVA
                     KGVDILLIDTAGRLQNKENLMAELEKMGRIIKRVLPDAPHETLLALDASTGQNALSQA
                     KEFSKITPLTGLILTKIDGTAKGGVVLAIRQELDIPVKFIGFGEKVDDIGEFHSEDFM
                     KGLLEGIL"
     gene            complement(33327..34151)
                     /locus_tag="VC122_000695"
     CDS             complement(33327..34151)
                     /locus_tag="VC122_000695"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002990612.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Cof-type HAD-IIB family hydrolase"
                     /protein_id="HEP5640639.1"
                     /translation="MNIRILALDLDGTLYNTEKIVTDANKKALAAAREKGVKVVITTG
                     RPLKAIGNLLEELDLLDHDDYSITFNGGLVQRNTGEVLDKSSLSFDQVCQIQQALEAV
                     GLPTDIISGGDVYSIPSKDGRHSQYHLANPLLTFIEVTSVAELPKDITYNKIVTVTDP
                     DFLDQQIIKLSPSLFEDFEAFKSRDIIFEIMPKGIDKAFGLNLLCQHLGLDARHVMAM
                     GDEANDFAMLEWAGLGVAMANGVSGAKAVADAVTTLTNDESGVAEAVKTFILEEES"
     gene            complement(34155..34952)
                     /locus_tag="VC122_000696"
     CDS             complement(34155..34952)
                     /locus_tag="VC122_000696"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011184306.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Cof-type HAD-IIB family hydrolase"
                     /protein_id="HEP5640640.1"
                     /translation="MIKLIATDMDGTFLAEDGTYNQEQLAALLPKLAEKGILFAVSSG
                     RSLLAIDQLFEPFLDQIAVIAENGSVVQYHGEILFADMMTKEQYTEVAKKVLANPHYV
                     ETGMVFSGQKAAYILKGASEEYIQKTKHYYANVKVINGFEDMENDAIFKVSTNFTGHT
                     VLEGSDWLNQALPYATAVTTGFDSIDIILKEVNKGFGMEHLCQALGIKKAETIAFGDN
                     FNDYQMLEFAGRAIATENARPEIKVISDQVIGHCNDGAVLTYLKGLV"
     gene            35256..36382
                     /locus_tag="VC122_000697"
     CDS             join(35256..35490,35490..36382)
                     /locus_tag="VC122_000697"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076612023.1"
                     /ribosomal_slippage
                     /note="programmed frameshift; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS3 family transposase"
                     /protein_id="HEP5640641.1"
                     /translation="MSTVKRYDEEFKQSLVNLYQTGKTQSELCKDYGVSASALAKWIK
                     QYSQVKLEDNSVLTAKQIQELQKRNAQLEEENLIPKKSKCHIHAKLKVRLLAVYRLRF
                     EHDTTTLCRVLRVNRSTYYKFLKHKPSKRELDNQIYRKQILEIYTKANKRLGVKSIKV
                     ILQRDYDTKISEGRIYRLMKNMALPKMATVKPKTALKRTQKTYPQNLLNQKFNPDKPN
                     QVWSTDFTYISIGYKKYVYLCAILDLYSRKCIAWKLSHHMDAKLACDTLELALNKRKI
                     EGTLLFHSDQGSQFKARKLRKIIDDNTIMHSFSKPGYPYDNAVTEAFFKYLKHRQINR
                     KKYQNIKQVQLDCFEYIENFYNNYNPHTANLGLTPNQKEEN"
BASE COUNT        11131 a         7818 c         6553 g        11320 t
ORIGIN      
        1 acaccttatt tttgccacag caaaaagcag ctgtttgggc tcatgtcaca cgcaaaaata
       61 agaaatcagc agagcagttc aagttttttg agcatcaaac tgggtttgtc tatattctaa
      121 aatctcctta aaggagattt tagaatatta ttgaaattgc ttgatattta caactttact
      181 attttctttt cgagtgtcct ttcgataaga taattcagct tctttaaata tatcatctcg
      241 cttttcgcta gcatcaatat ttagtacaaa acccactgca acagataaga caagtaagct
      301 atttccacct tgagacaaaa aagggaaggt aacaccagta gatggaatta agcctgagat
      361 accaccaata ttaacaaaga cttgcatgag catcataccg cctactccta aagccatcat
      421 ggcattaaaa ggattttttg ctttaatacc aacattcata atacgtaaaa ttaagaagaa
      481 aacaagtgcc aaaataaacc ctgctcctat tagacctaac tcttcaatga caactgaaaa
      541 gacaaagtct gtctgtgctt cgggcaaata gccacgcttt tcaatggaat ttcctaaccc
      601 ttggccaaac cacccaccat tactcatagc atagtaagaa ttggctaatt ggtgacctga
      661 atcggtcaag tcatgaaaag gattaaaaaa ggcactaaaa cgtttagcga catagccaaa
      721 aacaggaatc ttagcaactc tttctacccc aataacggca atagtcccaa gaaagaccgt
      781 agatagaccg gtaatcatga ctaggatagc cgaaaaccat cggtaaccaa tgccactgat
      841 agaaaacata ataatggctg tcaatacaat aatggaagca tttcctaagt caggctgagc
      901 agcaaccaaa agtaccatca acaaagaata aacacgccag tcccgtaaat cgttccattg
      961 tgttggccac cattttcgac gagttagtgc ttgataatca taaaggctaa tgtttttttg
     1021 gatcttagca aaggtcaatg ccaaatacca taccataatg atctttaaat actcagcagg
     1081 ctgaaagctg actggtccga tcacaatcca accatgcgct cctttgatag cagttgtaaa
     1141 gaagcgggca ataattaaaa gaaaggcctc tcctaacata accacagtta atacccttgt
     1201 attcgttaaa aaatttaatt ttaatttata gataaatgta atagccacta gacttattat
     1261 ccaaaaaact ccctggttaa tcaccgattt aaatggatta gcatgtgctt gaatcaagct
     1321 gacacttgtg gttgagtaaa ccattattaa gcctatcacc gataaaatta aataaggcaa
     1381 taatatagaa taatttaaga ggtgcctttt atcaatcttc atagttttcc ctctacttct
     1441 tgagataatc atttagcatt ataacatctt tctatatcaa acaaaagaaa aagtcaagaa
     1501 tcctcaaaaa aagaagctcc aagggctaac ctttaaaatt ctgattagtc acttgaaaac
     1561 ttctataaat accaattaat actcaagagc attagcctga attacgtaaa ccagtcgcta
     1621 tgccattgat agttgtatgg atcaattttt catcattttc gtcaagttga ttattacgcc
     1681 aacgtttaat caattcaatt tgaatatagt tcaacacatt aaaatacggc aagcgtgact
     1741 tcaagctatg cttaagggaa ggattatcct ccaatagatc atcatggtct tggatagcaa
     1801 gaataacatt ttttgtcaat tgccactcat ccaaaatttc ataaaaaaca tctcgaacat
     1861 cctgactttc agctagttgt gcatactgaa aggcaatatt catgtttgat ttagataata
     1921 ccatatcaac atttgataac aaggaatgga aaaaaggcca cgtctgatac atgtgttgga
     1981 ggcgttcaag gttaccttgt gctcggtcaa tataacgttt aaaggctgaa cctaccccat
     2041 accaacctgg gaacatgatg cgattttgtg accatgaaaa gacccaagga atggcacgca
     2101 agcctgtaat ttccgtaatc gttttacggg ctgctggtct tgacccaatg ttgagactgg
     2161 aaacttcctt aatcgggctt gcctcaaaga aataatcata gaaatgggga ttgtcaaaaa
     2221 ccaattttcg ataaatggta ttgctatcag caacaatgct atccatgatt tctcgaaaac
     2281 tatcaatctc attaggatct gttatcattt gagtcaccat acgattgata gaagctgaaa
     2341 tcaacatttc taaatggtaa taagccacat ctttattgcc gtatttgttt tcaataattt
     2401 ccccttgctc tgtcagacga atccgatctt tgatggaacc aaaaggttgt gaagtaatgg
     2461 catcataaga agggccacct cctcgaccaa ctgttcctcc acgaccatgg aagaaggtga
     2521 ttttcactcc atgttcctca ccaatagccg ttaattcatt ctgagcttta taaagagtcc
     2581 aacccgatgc caagtagcca ccatctttat tactgtcaga atagcctaac atgatttctt
     2641 ggtaattacg attcgttgca atccaagatt tgacaatgtc atggcttaaa taggctgcca
     2701 tgatgtcacg ggcattatct aaatcctcaa tggtttcaaa gaggggaaca atttgcacac
     2761 gagcccgctg ctggtcaacc aatcccactt ctttaagcat aatggccaac tcaaacatat
     2821 cagagacact ttctgtatgt gaaataatat gttgctttat aacctcttct ccaagcttat
     2881 ctttcaattc tcgtgccgta gtatagatag ccagttcttt ttctaatagg tctgatttag
     2941 caactgccgc tgaggataaa gtccttggct cctccattaa ttgttgtaag agaacatcgc
     3001 atttttcagt ttctgaaaga gagctataat cgtcaacaat attggctcct tttaataact
     3061 ctgccacaca agattcttgg acactggagt cttgcctcat gtcaatgctt gctaagaaga
     3121 agccaaaaat atcaacagct tgcatgactt ctcgcaaatc cccctcaatc aaagcggagt
     3181 tgccattatc aagtagcgac tgctcaatag cctttagatc agctttaaaa tcaacaggag
     3241 agtcataggc taagatggga ttttctaaac tagctggtga agaccaagca gatgtgctta
     3301 gtcccacact actactcata cgagctgctg gctgattagt tagttgaatt tgtgtttgtt
     3361 taagacgcga ttgaatataa tgaaaagctc tgcgataagg ttcatttcca cggtaaatcg
     3421 attggtcctg agacaaactg gctaaacgtt ccacttccga gttgggttgt accaaggtcg
     3481 acgacagtga aaatgttcgg taaagggctg ctaatttgtc aatgtagtaa ttgaggataa
     3541 cctcactttg aaccgtagct gacaagcaca aggtttcagc tgtgacaaag gggttcccat
     3601 cacggtcccc accaatccac atccccatgg taatgggttt gggattgtct aactctagac
     3661 cgtgtttttg ggctaagttt ttgtactctg ttgtcaactt ggtcactgct tgaatgagag
     3721 aaccatcgta atactgcatg acatttttta tctcgttttt cacctgaagc tttttctcgc
     3781 gaataatatc tgtctgcatg atcatttcaa tataacgata tagctcctga cgccatttct
     3841 caagattaat cacaccagcc ttagcatcac ggtatttgcg cagaagttta tgaatgtgag
     3901 ttgtcaattc cagaatcgtt ttacgttgta cttgcgtcgg atgtgctgtt aaaacaggaa
     3961 ccacattaac ctgctccaaa atgtctttac cattatcttt tccagcaaga tccttgatgg
     4021 ttagggctaa ttttcctaaa taatcagtat ccgtattatt ttgataattg atttcatagg
     4081 ctaaatcgac atcttctgaa atattgatta gcaagggtaa aatagaaaaa tacctggaaa
     4141 tcacttccat ttcttgattt gaaatattgg cgacaacttt ttctaattct atgtaatctt
     4201 gtttttcaga taacaccata atagactcga taactgcaaa cgcatcatct ccaatcatcc
     4261 gtcgtgtgat attttccaac atttctttca aaagagcaac ttcttcagca atgatagctt
     4321 gattgttact actctctaac tttttaagtg gcaaatgacc atctccttct ttgctagttt
     4381 cttctatcat accacttttt accaaaaata aaagtgatca tgttaaaaac gtttgttttt
     4441 taacaaaagt aagcgcttta ttttaaagaa tgtttggata ggtttagtct ccatattcca
     4501 ctaaaattct gttatactag tcttgactat attgttaagg agacaactat ggaattgaaa
     4561 aaacgttcag aattccctga aaatgaactt tgggatctaa cagcgctcta caaagaccgc
     4621 caagattttt tactcgctat tgaaaaagcc ttacaagata ttgacctgtt taagcgcaat
     4681 tatgaaggcc gcttaacgtc ggtggacgat ttcactcagg ccttaataga aattgagcat
     4741 atttatattc aaatgagtca tattggcact tatgccttta tgcctcaaac cactgacttt
     4801 tcagatgaaa gttttgctca aattgctcag gctggcgatg atttcatgac taaagctagc
     4861 gttgccctta gcttctttga caccgcctta gccaatgccg accttgacgt gttagataca
     4921 ttagaaaaga acccttattt cagcgcagct attcgcatgg caaaaattca aaaagaacac
     4981 ctcttaagtc ctgatgtgga aaaggctttg gctaacttac gcgaggttat caatgcgccc
     5041 tatgatattt acaccaaaat gcgggctggt gactttgaca tggaggattt tgaggtggat
     5101 ggtaaaacct ataaaaatag ctttgtttct tacgaaaatt tctatcaaaa tcacgaaaat
     5161 gctgagattc gtgacaaagc cttccgctct ttttcaaaag gtctccgtaa acaccaaaat
     5221 acagctgcag cggcttactt agccaaggtt aaatctgaaa aattgttagc ggatatgaaa
     5281 ggctacgctt ctgtctttga ttacctccta gctgaacagg aagtggatcg tagcttattc
     5341 gaccgccaaa ttgacctcat catgactgaa tttggaccag ttgctcagaa attcttaaaa
     5401 catgtcgccc aagtcaatgg tcttgagaaa atgacttttg ctgactggaa gctagatatt
     5461 gacaatgatc tcaatccaga ggtcagcatt gatggtgcct atgacctcgt gatgaaatct
     5521 ctagccccat tagggcaaga atacacgaaa gaaatcgaac gttatcagac agaacgctgg
     5581 gttgactttg ctgctaacgc caataaggat tctggtggct atgccgctga tccctacaag
     5641 gttcatccgt atgtcttaat gagttggact ggaagaatgt ctgatgttta tacccttatt
     5701 catgaaatcg gtcactctgg tcaattcatt ttttcagata atcaccaaag ttacttcaac
     5761 acccatatgt caacttatta cgtcgaagcg ccatcgacct ttaatgaatt gatgcttagt
     5821 gattacttgg aacatcaatt tgatgaccct cgtcaaaaac gttttgccct tgctcaccgt
     5881 ttaacagaca cttactttca taacttcatc actcacctat tggaagcggc tttccaacgt
     5941 aaggtttaca ccctgattga agaaggcggt acctttggag ctgaccaatt aaatgcgatg
     6001 atgaaagagg tcttaacaga cttctgggga gatgccgttg acatcgacga tgacgcagct
     6061 cttacctgga tgcgccaagc gcattactac atgggcttat atagttatac ctattctgct
     6121 gggcttgtta tttcaacagc tggttacctt aaccttaaaa acaacccaga tggggcaaaa
     6181 gaatggcttg acttcctaaa atcaggtggc agccgtacgc ctctcgatac tgcaatgctc
     6241 attggggcag acattgccac cgaaaagcct cttcgagata ccattcaatt cttgagtgat
     6301 accgttgacc aaattatcaa ttacaccgag gagatgtcac atgcctaatc tctatgattt
     6361 tacggttaag gctcaaaatg gaaatgacct ttccttagct gcttataaaa aaaaggttgt
     6421 tttgattgtt aacacagcta ccaagtgtgg attaacccca caataccagg ccctgcaagc
     6481 actatatgac acataccatg acaagggatt tgaggtctta gattttcctt gcaaccaatt
     6541 tttaaatcaa gctccaggag atgctgagga gattaatcac ttttgcagtc tgacctatca
     6601 cactactttc cctcgctttg ctaaaatcaa agtgaatggt aaagacgctg atcctttgtt
     6661 cacctggtta aaagaagaaa aaagtggtcc ccttgggaaa cgcatcgaat ggaatttcac
     6721 taaatttctc attgaccaaa atggtcaagt cattaaacgc tattcctcaa agacagatcc
     6781 taaactaatt gaagaagacc ttaaagcact tcttggttaa tataaaatag acttggcgtg
     6841 ccaagtctat tttgtattaa tagttactat ttaaaaactt cttgataaaa ttcaatgcta
     6901 tcaccatctt taacggtaat ttgatcggca cccttatctg ccatctttcc attcacctta
     6961 aaaagccagt atttgttagc tttcgtgtct tgctcaatac catcaattgc tgtgataaag
     7021 ccatcttttt ctttgacctc ataattatct ttgagaatct caagcaccgt atctcctttt
     7081 ccaaaagaaa ctttttcatc aatggtattg gtatcttctt tgacaactaa gcgaacgtga
     7141 tggtctgctt ttggaacgct tggagtctgt gtttgttttg ttccttgcga gcaagcaaca
     7201 agtagcatag caacaagcgc tagaaatcct gttttaaatc ttttgttcac cgattaacct
     7261 cctaaataac atcatcacaa ctggaaaaaa gacgagagtt gataaagcat gcgctatatt
     7321 aaagggcatc cctgctaaaa cataactcca ccaaggcata ctatacaaat acgcaaaaca
     7381 agtatctatc aacaccccat aaaggaggcc acataaggcg gccaagaaag cctgataacc
     7441 aagctgatat ttggggaatt gctgagacaa agggtacaag gcaaaacgcc ataataaaag
     7501 aacaagcaca aaacaagtaa cttgccaaaa gacccaaggg ccaaatccca gcaaaaaggc
     7561 tgataaaaac aagcacaaca tcataactaa tacagcctcg gctaacccaa agtaaagaag
     7621 ataactgagt aaaaatgccg tcacaggctg cacattaggc aggctgctaa aaaccattct
     7681 caacacaaca catagggctg acatgatagc aatgcgactc ataaacttag ctgacataaa
     7741 cttcttcctc gtttttttct ctattatgac agatttacag agcgtttacc agtttgaagc
     7801 tcacttattt atcaaatgat aataacatga gaattaaata acaaaacagt acttgctgac
     7861 tgtcatgata tagtcaaaga ctaaactaat attgtaaaga atatagctac caactataag
     7921 agatagtgac ataaacttga catagttaga gaggatctca tttgtaactt ataaaagata
     7981 ataccttaaa atgcaagata atataagaaa acagttagga tattttgatt ttttttagta
     8041 attagggtac aataacagtt aatgagaaga aaaataaaac ctattgttgt gctagtcttc
     8101 tttattttac tggcaatggt attgattatc ggaaaaagac aagctaatca tgcgaaacaa
     8161 aaagaagttg aagacgctaa gagtcatatt cctattgcaa ccagtaatcc tagtaaagca
     8221 aaaactagca cttctgaaac cgaagacttt attttaaatc ctattgttga tgtttccggc
     8281 tggcaactcc ctgaagaaat tgattatgac acattgtcac gtcacatctc tggcgcaatt
     8341 gtacgtgtct atggaggatc gcaaattaca gctcataaca acgctgcttt tacaactgga
     8401 attgacaaat cttttaaaac gcatatcaaa gaatttcaaa aacgaaatgt tcctgtagct
     8461 gtttacagtt acgcacttgg tcgtagtact aaagagatga aagaagaggc ccgagctttc
     8521 tacaaaaatg ctgctcccta taatccaact tactactgga ttgatgtcga agaagccact
     8581 atgaaagata tgaataaagg cgtaacagcc ttccgagaag agcttaaaaa acttggtgct
     8641 gaaaatgttg gtctctatat tggaacttat tttatggcag aacaagatat ttcaacaaaa
     8701 ggtttcgatg ctgtatggat tccgacttat ggtagtgatt ctggctatta tgaggctgct
     8761 cctaatacaa ccttagatta cgatttacac caatatacct cacaaggtta tctcagtggt
     8821 tttaacaatg ctttagattt aaatcaaatt gccgtaacaa aagacactaa aaaaacgttt
     8881 gagaagttat ttggcaactc caacaattaa gaggttagca tcatggctaa tcttttttta
     8941 tagtttacat cttaccaaga aaaactctag gtcaataatt taaaacccct tatccttgac
     9001 aaggagatta tctcatcata taatgaaagc actataatat ttcacaaagg agtgctttgg
     9061 ctgagatcgc aattgcgaaa tcctgaggac ctgatcttgt tagtacaagc gtagggattg
     9121 tgaccaataa tcaatgattg gaaagcgata ctctttttgt gcacaaaagg agttttttat
     9181 gtcaccaaac accaatgtca aataccttat cgaagctgct atttttgctg cactggcgat
     9241 ggccctttca tttattccag attttgctgg ctggtttagt ccttcttatg gtgctattgc
     9301 actggttatt ttctctttgc gccgtgggtt aaagtacggc atgttggcgg gtctcatttg
     9361 ggggctgctt cactttgtac ttggaaaagt ttactatctc agcatgtccc aagtctttat
     9421 tgaatatatc ttagccttta cctctatggg attagccggt agtttctctg atagtttgat
     9481 taaaacttta agaagacaac aaaccttttt tgccgttttt cttgcaataa tggctagttt
     9541 actagccgtt acagtacgtt acttgtggca cttccttgca ggaatcattt tctggggcag
     9601 ttacgcccct aaaggcatgt ccgctgtctg gtattctttt agcgttaatg gtacagctgg
     9661 tgtcctaact tttttaataa catgtttagc gttaatgatt gctttaccaa ttcatcctca
     9721 attatttgat cccaaagact aatttttagt catttgaatc ttacattaaa aaaatggtat
     9781 aataatgttg ttataccatt ttttatgttt agataaaggt aaggacttga tatggctaaa
     9841 aaaacaaaat tcaaaaaaac cttagtagaa caaatcctcg ataaggctaa tattgctcat
     9901 caaggactca agcttaatgc tttagaaggc gactttccag atgatcttca gccatcagat
     9961 atttacaaga cacttgctct gactggtgat caaacaggtc ctctaattgg cattattcca
    10021 ttaacagaac acttatcaga aaaacaattg gcaaaagtat caggtaacaa aaaagtgtcc
    10081 atggttcctc aaaaagactt gcaaaagaca acaggctata ttcacggtgc caataatcct
    10141 gttgggattc gtcaaaaaca ttcatatcct atttttattg accaaactgc actggaaaaa
    10201 ggtcaaataa tcgtttcagc tggtgaagtt gggcgttcta taaagatttc tagccaggct
    10261 ctagctgatt tcgttggcgc aagctttgct gatttaaaga agagaaaatg aacactactg
    10321 ccaactttat ctggtttctt ttttaagttt ttcgccttga ttaagctagt ctttgtttag
    10381 tattattttg atctcaactg ctttaacatt gatcctgtta atctttttct tttgtaaagg
    10441 cttgatatca cgacaaattc aggtcgattt gtcttaaatc taatatgggc tttacacttt
    10501 aatcagaacc tagctattat acacttatac tacgcttttt tactttatct tttcactata
    10561 ggtattattt ttgcttggtg atgcctaaac tttttctggt tactttttta tttttagcgc
    10621 tcgcatcagt tatctctaaa aggaggctta aatgaaatta tattttgtaa gacatgggaa
    10681 aaccctttgg aatttagaag gtcgctttca aggcgctggg ggcgattcgc cattacttga
    10741 agaggcaaaa gacgaaattc acttacttgg caaagagtta gctaaggtgg cttttgatgc
    10801 cgtttatact agcgaccttc aaagagctat ggcaacagca gctattatct tagatgcttt
    10861 tgaccaacag ccaaaacttt accacaccga ccagcttcgg gagtggagat taggtaaatt
    10921 ggaaggggct aaaatagcaa caatggcagc tatatatccc caacaaatgc tggcttttcg
    10981 ggagaatcta gcccagttca aatcagatca gttcgaggct gaatcaattt accaaacgac
    11041 tcaacgtgtc tgccatctta tccaatcgtt taaagataaa cattatcaaa acgttcttat
    11101 tgtaggacac ggcgctaact taacagcaac tattcgctca ttattaggtt ttgaacctgc
    11161 tcttttactc gctaaaggtg gcttagataa taccagtctt actattcttg aaactaagga
    11221 ttatttaact tatgactgtt taatttggaa tgataaatcc tttctagagg tgtaaaacca
    11281 ctagacaata ataaaaaaag ctaacacatt gatttcttac tgacaagaca ctcactttgc
    11341 tagctttttt tattattatc tagtgggcta ttatggtttc atctatctgg catattttaa
    11401 gcgcaaaact ttttcttgct gcttggttaa ccgaagcaag ataaccacct tgtcaatgct
    11461 catgttactt tcaagtaaac gctctgctgc catcatcaag ccattattga ttcccatctg
    11521 taaaattgga tttttcttgt cattgacatc aaaaataaat ttattatcag gtaaatcaaa
    11581 gagtacttta acggcaccaa agagctgttc aaagttatca atagccacat cataaacagg
    11641 cttagtgcct ggttttagct gacgacctct atgattaaac cacatggaat gccaaccacc
    11701 attaaaggca cccatgatat cattgtcata agagtcccca acatatagtg tggtttgtgg
    11761 gttcatgtca aattgttctg ctgccaggtt aaaaatttct ttttctggtt tttgaaagcc
    11821 tgttgcttgg ctcacaatga cacgcttagg atcaatataa tcataaagcc ctaatttacg
    11881 aacttttttc agctgatgtt ctgtgggccc attggtaata atccccatag gaacattctt
    11941 tgattttaaa aagtcaaggg tcatacgcat ctcatctaac atggtaatgt tctcaagttc
    12001 gtgctcatag acttcttgaa aatgtactcc cgcagcttcg tcgatttctc gatagccaaa
    12061 ctcaagcagt gtttccttgc aacgccagaa tcggaaatat tctgtggtcc actcccctgc
    12121 cattactctt gggaatccaa catctgaata gtgacgaaag cgaatataag cttgattcat
    12181 tactgacata tcaaaatctg ggaagcactt ttccatggcg attcgataag gcgcttgctg
    12241 gtcgtaaatt gtgtcatcca catcaaaaac aatagcagta atcatgagtc tcttttcttt
    12301 tctagtttaa tatcccaatc attatactat tttccattag gcttttcaat ttcattttca
    12361 aaaaaatgcc ccgctgcagt cgcattttcc aacgaaatat aggtgtattc tagccccttt
    12421 tatgatacaa tggaaaggaa atcgtattgg aggaaaatat gtctaatcaa cacatcgaag
    12481 aattaaatga ccaacagatt gtccgtcgtg aaaaaatgac ggcgctggct gaacaaggaa
    12541 ttgatccttt cggtaaacgc tttgaccgca cagccaattc tgctgaatta aaagaaaagt
    12601 atgctgataa aacaaaagaa gaattacatg agttaaacga aacagctatt gttgctggcc
    12661 gcttgatgac aaaacgtggc aaaggtaagg ttggttttgc tcatcttcaa gaccgtgaag
    12721 gccaaatcca actttatgtt cgcaaggact ctgttggcga agacaactat gaaatcttca
    12781 aaaaagccga tttaggcgac tttatcggcg ttgagggcga agtcatgcgt acagacatgg
    12841 gcgagttgtc aatcaaggca accaagttga ctcacctttc taaatcactt cgtcctcttc
    12901 ctgaaaaatt ccacggatta actgatattg aaaccattta ccgtaagcgt caccttgatt
    12961 taatttcaaa tcgtgaaagc tttgaccgct tcgtgacccg ttccaaaatg atttcagaaa
    13021 tccgtcgtta tttagacggt cttgatttcc tagaagtaga aacgcctgtg cttcataacg
    13081 aggctggtgg tgctgctgct cgtcctttcg ttactcacca taatgctcaa aacattgata
    13141 tggttcttcg tatcgcaaca gaacttcatt tgaaacgttt gattgttggt ggtatggaac
    13201 gtgtctatga aattggtcgt atcttccgta acgagggaat ggatgctact cataatcctg
    13261 agttcacttc tattgaggtt taccaagcct atgcagacta tcttgatatc atgaacctga
    13321 cagaaggcat tatccaacat gctgctaaag ctgttaaagg tgatggccct attgattatc
    13381 aaggaactga aatccgtatc aatgaacctt tcaaacgtgt ccacatggta gatgctatca
    13441 aggaagtgac aggtgttgat ttctggcctg aaatgactgt cgaagaagct attgcacttg
    13501 ccaaagaaaa acaggtccca ctcgaaaaac acttcacaag tgtcggacat atcatcaatg
    13561 ctttcttcga agaattcgtt gaagaaacct tggtccaacc aacctttgtg tttggtcatc
    13621 ctgttgaagt atcgcctttg gctaagaaga atccagaaga tacccgcttc accgaccgct
    13681 tcgaactttt catcatgacc aaagaatacg ccaatgcttt cacagagttg aatgatccca
    13741 ttgaccaatt atctcgtttt gaagcacaag cacaagccaa agaacttggt gatgacgaag
    13801 caactggcat tgactacgac ttcgttgaag ctcttgaata tggtatgccg ccaaccggtg
    13861 gactcggaat cgggattgat cgtctttgca tgttattaac aaacacaaca acaatcagag
    13921 atgtgctttt attcccaacg atgaaacctt aactttttta agatttgtat tcatatctta
    13981 aaaccgataa gtggcaatat aacataacgt tttattatct ctaaccagtc ttcattacca
    14041 tgaagactgg tttattaaca gtagcttata gaaagtgact taacaagatg accaaagaat
    14101 atatctccta cattagtgct gctatcttct tgctttatgg ccttttagct gccaaatgga
    14161 tttttcttgg gctaggctta attttggtac ttgtcggcct tgctgatcgc ctcaattcaa
    14221 aaaaataatc cttctattcg cccctgtcct tttaacctag cgatgtcatc accaggttaa
    14281 aaggacaagg gcgaacgagg gattatgttt cgattaaggt aacattactt acgaaaagcg
    14341 tctaagatgg ttttaaattc ttcattagta agagtaatgc ctttccccat tttcgtatgg
    14401 tctggactcc acgtgcggat atcccatttc gcttctgcgc cattaaagct gacgcggttc
    14461 aattctttcg tccaaccttt atcattttct gataaggtga gtaaatgttc ctcaatgttg
    14521 aatgtaaatt ctgccattag ttttcctcct catctatact attcgaaatt aaagtggaaa
    14581 aaatcatctt tttttcactt tttttatgac tcattctcct attattctat caagttttga
    14641 gaaaataagc tattgtgata tccaattttc aaaaaaaaaa aaaaacaggc tctcttttcc
    14701 caactatggt cgtataccat ttggaaaaag agggcctcta atatttcttg ttactatgtt
    14761 ctaacagttt tactggtgcc atctttttga tagaggttga caaggccttc cttgcaagcc
    14821 ctaatcatat cccctggctt aacttctctc ggtaaagaga tggttaagag ttccattgga
    14881 tttggggcac ggtcaatctt ttggccatcc gcatcatgta agtccttaac aaccgtttca
    14941 aaatgacgga aacctggtcc ataacattca atccgatcgc cttccatgat gacattacgc
    15001 tgacgaatgg tcgctgtcat gctagctgag tcaaaggcaa ctacttctcc gacaaattta
    15061 tattgtggaa ttttgcggcg agcaccaaat aattgttcat tttcagttgg gataccataa
    15121 taaaaacctg tagccaactc gcgctgggca accttccaca actcgtcaat caattcctct
    15181 ttgatagcat aaaaagcttc tgggctttcc atgtaagcat ctacagccgc cttgtaacag
    15241 ttggttacgg ttgagacgta gtggatagat ttcattcggc cttcaatttt taagctatca
    15301 accccatttt caatcaggtc aggaatatgg tcaatcatac acatgtcaac agaggacata
    15361 gaatagtctt ctggaatttc cccttttaag gagcggcgct ctcctccaaa tggcatgtca
    15421 tacaaatcat acttccagcg gcaagactgt gagcagccgc ccctgttggc atcacggtga
    15481 ctcatgtggt ttgacaaaac acagcggcct gaataagaaa tacacatggc tccatggaca
    15541 aaggcttcaa tttccacatc tgtccgctta cggatttctg ctaactcggc catattaacc
    15601 tcacgagcta aaacaactcg ggtcaaaccc atggctttcc aaaattcaaa ggtctcgtaa
    15661 ttggtagatg aagcttgcgt tgacaaatga atttccaaac ctggggcttc tgttgaacaa
    15721 ataataatca aggctggatc tgaaacaatg accgcatcaa gccccatatc acgcagttga
    15781 cgaaaccact cgcccgcacc aatttcgttc ccttcgtggg taaccatgtt ggcagcaaca
    15841 tagaccttag ctccacgcgc atgtgcataa tcaatgcctt cttgcaattc ttccatagag
    15901 aagttaccag cgcggcttct tagaccatag gcctgtcctc caacaaaaac agcatctgcg
    15961 ccatagtcaa tcgcaacttt taatttttca agtgttccag caggtgataa gacctcggga
    16021 cgttttttca tatgtgacat gtgatattct ttctattttc aagataggta gattacctgt
    16081 gtaatggtgt cataaccctg tcttgccctg aaatagtaaa ctcactcaag acaaggatta
    16141 cttatttaac ggtttttgga tcgaactcat aaaagccagt atctaatcca cggccagctg
    16201 gatgatgggc atgcactgct tgatcaagct tttcagcttc ttcttgggta aattgcccag
    16261 cttctaataa ggttttagcc tgaataaaca atttagtaat agcgacaaaa tcatcacctg
    16321 gacagtaaat gccatctagc ttccaatgag ttaagccatg cgcatacagc tcacccaatt
    16381 tagacatcat gtcgatatcg ttattaatga aaatatgagt gccatgattg tcttcataaa
    16441 tggagtaatg gctgctagca tctcctggtt ctgccaagaa aagtcctcgt tcacgactaa
    16501 cctcatcatc aatctttgta aaatggtaat aattttctag caagggacgt ttcgagtgat
    16561 gaatcaccga ggcgccgtaa actagcacct ctgctggaaa ttccaaattt tcagctaagg
    16621 tgaataactc tgctgatggg atttcccttg ctaaaacact ttcaacagca ccatgttgcc
    16681 cccagaaatt tacctgacgg ctagaagtca caaaaacaga ggtatcataa atcagtttaa
    16741 agttatagcc atccctttta ttgacataaa acacacctgc atcaccaacc acaagataat
    16801 caacagcaat ctctatcatt aagtctaaaa agggtttgat ctgatccatc atatcttgat
    16861 gcatcaaagc attgcaagct actgtcaact ctttgcctgc atcatgcact agtttggcaa
    16921 tttgtctcaa ttcatcataa gaaaaattat ggggaaggcg taacccgtaa ttggcttcac
    16981 caacataaat acgatcaaca ccagcagcta ataaagcttt gacttgctca atgctttcag
    17041 ctgtggctgt aataattatt ttttccatat aagctttatt ataccaaaaa ttttaagacc
    17101 ttaagcatat ttcttgcaaa agcccaaacg ttaagctagt ccccttactg ttatagcatg
    17161 ataagtcact tgtcattagt aaatcttttc aaatctttta aaaaaagatg acaagaagag
    17221 acttttatga tagaattact tagtatgtta aggaaaggag aggactatgc ctgcaccgtt
    17281 aaagaagcat aattcggttg aacaggatga ttcttaccaa gaatctcaaa gtaaatttcg
    17341 agattttcaa gagttcaatg gccaaagtgc aaagctaaaa gaattagtct tctttgctcg
    17401 tatcgctagt ttcagcctta ttaccgtttt atttgcattt gtcctgctcg taatgaattt
    17461 agcgccaatg tttgctttct tatttgcaag catcatcagc ttaggcatta cttccttatt
    17521 cgccaaggtt atcaaatcat tactgatgta acttccagag tccgttttac gtgactcttt
    17581 tttttgtgtc aaaaacaaaa aaagattgaa gccacaatcc cccccttctt cagttagcct
    17641 aaaactaacc atcgggacta gtgatcagat ccttcaatct tttcttcttc tatgttttag
    17701 gtcgttttag ttagtgttta tctagtgtat cactaacagg ctcatcttgt tcagtatagt
    17761 caatgatgat gtcttcttca tttaaatcaa tgataacatc atcttgcttt tctttcgctt
    17821 caacttcggc aagggtttct tttgtttttt gcacgaaagc tgttgtttta tctttgagca
    17881 agtctaacat gtcctcttta gttaagtcac cagaagccaa cttatctttc acatcataga
    17941 aagtgtcacg tgctaaatgg ctgtattcac tacctttttc tttagcaaat tggtggtaat
    18001 catctggact ttctttgtag gcttgataag ctttttcagc acgatttttc aaagccttgc
    18061 ctttttctgt tgataagaag taagctgccg ctactcctga aacagctcca atcactaagt
    18121 ttttgaaact tttattcatg cttttctcct ttttttccaa atacttttga ggctacttta
    18181 ccaactaatg ctaattttcc agcttttgaa acattacctg tagcattagt tgctttttgc
    18241 ccaaaatgcc ttgcttgact atttaaatct gaaagacttt ctgacaaatc agcaatagca
    18301 acaaacagtg gatcgatggt tgctacttta ccattaacat cttcaacaag gatattagct
    18361 tttgctaaaa tatcattagt ttgatgaagt gtcacattaa catcacttgt caatacagaa
    18421 atcgtttttt tggcttcatc aattgtttct gagactttct ttaatacaat aatcaagaaa
    18481 atcaccaaag caacaaatgc gagtgcaata atcatcagtg agattccaac taagtccata
    18541 agtttctcct ttttctatcc ttattcttga taataagaaa ttcctttttg acggcgtctt
    18601 tttatgacaa agattaagcc aataataatt aataaggcag acacatattg ggagatgcga
    18661 atacctaaaa acataagact atctgttcgc ataccttcaa tgactagtct tccactacca
    18721 taccaaatta agtaaaatgc aaagatttct ccatctaata gactttttgg cttacgacgc
    18781 cacatcataa taatgacaaa tcccaaaaga ttccaaagtg attcatagag aaaggtcgga
    18841 atgcggtaac ttccctctat aaacatttgc ttttgaataa agcttggtaa gtaatttaac
    18901 tggctcacag ctttaccata ggcttcttgg ttgataaaat ttccccagcg accgatagct
    18961 tgagcgacca tgacacttgg tgcggcaata tctaaaaaat gaatgggatt gagcaccttg
    19021 ttataacagt aagccaaaag tacaagagct cctgtaatga gaccgccata aatagcaata
    19081 ccgccattcc aaatggctat gatttcatct aaatgcttga cataataaga ccattcaaaa
    19141 ataacatagt aaattcttgc accaacaata gcaagtggaa aagcaatcag aataaaatcg
    19201 aaaatagcgt cagatgatat tccttttttg ggtgcttctt ttgatgctaa gtaaaccgcc
    19261 aaaacaagtc ctgacaagat acatagcgca taccagtgga tggctagtgg accacatttt
    19321 aaggcaatgg gattgatcat tgactcacct catttttggt gattagttga gttaaacgat
    19381 cttcaaaagt ttttgtagca tcaaagccca tttctttagc ccgatgattc atcgcagcgg
    19441 cttcaataac cactgaaaca tttcgacctg tttttaccgg aatacgaata cgagggattc
    19501 tcactcctga gaaagttatc tcctcattac cgtttccgag acgatcaaaa actttaccag
    19561 cctcaaagtt ttcaaggtaa atggccaatt gaacttgtga tgagtccttc acagcactag
    19621 ctccataaag cgacatcaca tcaataatac ctaccccacg aatttcaagc aagtggcgca
    19681 agatctctgc tggctctccc caaagggttt cttcatcttt ggcatagaca tccacacggt
    19741 catctgcaac caaacgatgc cctcgcttaa ctaattctaa accagtctca cttttaccaa
    19801 ttccagaatc cccttggata agaaccccca taccataaat atccattaaa acgccgtgaa
    19861 cactagtacg ttcagctaaa gaagcgtcaa gaaaataaga catttctcca gctaagcggc
    19921 tagttgaaac acggctactt aaaagtgaaa taccttcttc ttttgcagct tgaaccattt
    19981 cttttggaat ggctaaattt ctagagacaa ccactgcagg agtgtccttt ttgaacatct
    20041 cttttaaaac agaataacgg ttgtgagaag tcatctgtgt aagataagac cactctttca
    20101 ttccaaatag ctgcaacctt tctggagcat aataatcaaa ataaccagtc atttcaagac
    20161 ctggacgtga aatatctgat gttgttatct cttttgacaa caagttatct gtagcataaa
    20221 ccacatcaag tttaactttt tgcactagca tctttacagt aacggtcata atttctcctt
    20281 tttcaatatt ataacacaaa tcgaccgtta gacccttgca aatactcatt ttttcactac
    20341 taaatgagaa caacgacacc aaaaatggtc ttagctctat taaaaactag gttttatcat
    20401 ttagagatat gagtcaaaaa taagacaatg gcaaacaaat actttactag tatcattgag
    20461 caattagtgc ttattatggt gtttaaaaag gtatcatctt actaagtcaa aaagcaaaaa
    20521 gcctcatcat agctgataag acccctcgct tacttacata tcaaaacatt ttattatcat
    20581 cagttaatca catggttgct cttacgtgat gaattatcat tttaactgat tgtttaccag
    20641 aaccagccat cttcttcttc atttaagaca tcagcgtctt tacgacgacg aggacctcgg
    20701 ccataacgtt cttcaagcaa gtcttccttg taaggtaaga agatggccaa caaaatataa
    20761 agcaaaactc caaaaccagt tccgtaaata agaagagccg ctaagacacg cgctagagca
    20821 agatcccaac catacttatc tgctaaacca gcaataacac ctgcaaccaa gcgatttttt
    20881 cgctgtttat agaatttagt ttccacgttt ctttacctcc tcattctatg atactagttt
    20941 acattgtttt tcgcaaaata aaaccagtct tacgactgat ttttttccat aagtttcccg
    21001 tggcaatttc cacaaacata tttagccaga ttgatacgcc tttttcgttg ataaacctgc
    21061 ccacaggttt ggcaactata atgatgatta gtctttgatt tagagctagt tgggacataa
    21121 cgaagcccgt ttacttgagc tagcagatct ttgaaatccc tatctttatg atggtaccct
    21181 cttccttcaa aataaagatg gtaatggcag agctcatggc gaacaatttt ccgaaaaata
    21241 agctcaccgt gctcctcaag catcctaggg ttaaaatcca aatgaccatc ttttggaaaa
    21301 aagcgaccgc cagttgtttt taggcgcttg ttccaataag ccttgtggtg aaatggtttg
    21361 ccaaaatcag ccagggaaac ttcttggaca taattagtga gtgtcacgcg gtggcaatag
    21421 gctgagattg accttgtggc ggtccaagtc aatcttagag acccaaacag ttaccaagtc
    21481 acccactgaa acaacttgac tagggtggtt gacaaaggtt ttgctcattt cagaaatgtg
    21541 aataagccca tcttcatgaa caccaatgtc tacaaaagca ccaaagtcaa cgacgtttct
    21601 cacagttcct tcaagcttct ggccaatttc caaatctttc aaatcaagga tatcttgacg
    21661 taagattggt gcttcaaagt catcacgaag gtcacgacct ggtttaagga gatcagcaat
    21721 gatatcttta agggtttcct gcccaatagc caatgtttct gccatttggg gaacttgaac
    21781 tgctgctaaa gttgccttgg cagcgtcgtc caagtcctga ataccaagta ccttaaagag
    21841 ttctttgact gctgggtagg actcggggtg aactcctgtg ttatctaaaa tatttttggc
    21901 tcctggaatt ctgagaaaac ctgctgcttg ctcaaacgcc ttagctccta aacgcggcac
    21961 ttttttgatg tcagcgcgtg atgtcaatgc tccattttct tcgcggtatt tgacaatatt
    22021 ttctgaaatc gttttgttca aacctgacac atgtgctaat aaagatggac tagccgtgtt
    22081 cacattaaca ccgacttggt taaccacagt atccacaaca aaaccaagat tctcactcaa
    22141 ctttttctga ctcacatcgt gctggtactg accgacgcca atagatttag gatcaatttt
    22201 aaccaattct gctagtgggt cttgcaagcg acgagcaata gaaatagcag agcgcttctc
    22261 cactgtgagg tctggaaact catgacgggc taactctgac gctgaataaa ccgaagcacc
    22321 gctttcgtta acgatgacat aggacgtatt tgggaaatct ttcaaaacgt ctgctacaaa
    22381 ggcttcactt tcccgactgg ctgttccgtt tccaatggca ataatatcaa tctggtaagt
    22441 ctcaatgagc tgagtcagtg tttctttagc tgcttgaatc ttcgtttggc tagctggtgc
    22501 tacgggataa ataacctgag tagtcaacaa ttttccagtc tgatcaacga tagccaattt
    22561 tgcacctgtt cgaaaggcag ggtcaaaccc aagaaccatt ttccctttta aaggagacac
    22621 taacaaaaga tgacggagat tttcagaaaa gaggtggata gccccatctt ctgcggcatc
    22681 actgagttct gaacgaaccc gtctttccat agctggaaca attttctttt tgatggtctg
    22741 attgatgacc tcttcaatat aagggttggt ttctttgaac cgcacactga aaaagcgttg
    22801 cattttctcc aggttatgct caaaagacac ttttaagatg cctaactttt cgccacggtt
    22861 aagggctaaa gtacgatatc cttgcatgtt agacacttgg tcagaaaaat cgtaatagat
    22921 ttggaaaacc tttttctcat ccaactgctc atctttaagc gttgatacta aacggctgta
    22981 ctgccaaatc tcattgtagg tccaagagcg taatttagca tcttctgaca tggcctccac
    23041 aagaatgtcc acagctcctg ctagagcttc ttgtgggctg gcaaatcctt ctgtgacaaa
    23101 gggttctgct gctgtttcaa ggttttgcgc attttgtaaa atcaaacgtg ctaatgggaa
    23161 caagcctgct tcacgcgcaa tcgtcgcttt tgtacggcgt ttttccttat aaggcaggta
    23221 caactcttcc aaatcagcga gtttttcggt tgcttcaata ctcgttcgta actgatctgt
    23281 cagttttccc tgttcctcaa ttttagcgag aatggttgct ttgcgctcat tcagggtagt
    23341 gagagactta tccatatcaa taatggactt aatcaccact tcatctagat ttcctgttac
    23401 ttctttacgg taacgtgcaa taaagggaat ggtattgcct tgtgctgtta gggcaagcac
    23461 ctgttcaatt tgatggagac taactgacaa agcctctgca atgttgtgat tattgttatt
    23521 ttccataata ttgctattat aacacaaacc atgttaatgc aaaaaatgat agcgaaacat
    23581 tgtcctaata taaagttgcg taaagaatca aaaaaagcta cttttagtaa ataactgacg
    23641 aaaccctaaa taacaatacc taattttaaa aaagaaagaa gtaaacagca cttccgcttt
    23701 ggcattgttc tcaaatgata gtataataga cttatcttga atgaaagaag gtcatagata
    23761 tggcaattac ccataaaaaa aatgatgaac ttgaaaaaat gctagcaggc tttgcatcaa
    23821 tccctagttt tgataagcct ttggaagtca atactgatgg taaactagcg accaaagaaa
    23881 aaacgacaga acaaacaaaa ccaagcaaac catagctagt ttgtctcatt ctcttaacat
    23941 atcgtccatt cgtttaaact gagttgagag atttttagcg atcaggctaa caaaacgtat
    24001 cagtcgaggc tgaacattcg tgtttcagcc tcctctttta tcatctagaa aggatatggt
    24061 gaatgtcact cttttccaac cttcctccca gtgtcttgca atggtttgcg atttttatgt
    24121 ccattatcat tgaagctctt ccttttgtgc tcctaggaac tattttatct ggttgtattg
    24181 aagtctttgt aacgccagaa ttggttcaaa aatacctacc taagcaaaaa tgcttgcgca
    24241 ttctgtttgg aacctttgtt ggatttgttt ttccttcttg tgaatgcggg attattccca
    24301 tcatcaaccg ctttttggaa aaaaaggtgc ctagttatac ggccgtccct tttctggcaa
    24361 cagcgcctat tattaatcct attgttttgt tcgctactta ttctgctttt gggaattctt
    24421 tgcgctttct gatacttcgc ttggtcggtg ctgcactagt agccattaca cttggggtaa
    24481 tgttagcttt tattgtagat gataacattc tcaaggataa tgctcaaccc gtgcattttc
    24541 atgattattc tcatgaaagc ttgccaaaac gcatttatct agcactggta catgctattg
    24601 atgaattctt cgacaccggt aggtatctcg tatttggaac cttgattgcc tctgccatgc
    24661 aaatatatgt cccaactcgt gtgttgacaa cgattggaca caatcccttg acagccattt
    24721 tgatcatgat gctgatggct ttcattctat cattatgtag tgaagccgat gcctttatcg
    24781 gagcttcctt attatccacc tttggagtgg ctccagttct tgcctttctt ttaattggtc
    24841 ctatggtgga tattaaaaac cttatgatga tggtaaagac ttttaaagga cgcttcattg
    24901 tccaatttat cggcgtatcc gttcttatga ttgccgttta ttgtttactt gtgggggtgc
    24961 tctaatgatt cgctttttaa ttttagctgg ctattttgaa ctaaccatgt atctccagct
    25021 gtctggcaaa ctggatcagt acatcaatgt tcggtattct taccttgctt atatttccat
    25081 ggttttatct ttcattttag ctcttgttca actctatacc tggatgaaaa acattaaggt
    25141 acatagccat ttaaccggaa aaatcgcaag gctgaccagt ccttttatct tggtctttcc
    25201 tgttttgatt ggtctccttg tgccgaccgt aaccttagat tccaccacgg tttctgccaa
    25261 gggttacact tttcctttgg ctgcgggagc ctctaaaacg ggagtcagtg atgacgggac
    25321 caccattcaa tacctcaaac ctgataccag cctttacttt acaaagtctg cctaccaaaa
    25381 ggagatgcgt caagaactcc ataaatataa aggcaagaaa ccggtcacca ttaccactga
    25441 aaattatatg gaagtgatgg aattgatcta tctctaccca gatgagtttc ttgatcgtga
    25501 catccaatac actggctttg tttataacga accgggccat gacaactacc aattcctttt
    25561 ccgcttcggc attattcact gtattgccga ctcaggagtg tacggacttt tgaccacagg
    25621 taatcaaacc agctacccta acaacacatg gctaacagtt aaaggtcgcc ttcatatgga
    25681 atatgataaa aacttagaac aacatctacc tgtccttcaa cttactgagg ttcatcaaac
    25741 caaagagcct aataatcctt atgtttaccg cgttttttaa agactaataa aaaagcactt
    25801 gttttttatc aaaacaaatg ctttttgagt tttatcagtt agcataggaa cagagtcacc
    25861 atcttaaaca agacctattt aggaaaaatt atcctcaagg taacgtccat tggtttgaat
    25921 gacttgtcgg taccaatcaa acgatttttt cttatagcgt gtgagttgac cagtaccatc
    25981 atcatttcgg tcaacataaa taaaaccgta acgcttactt aattgggctg ttgacataga
    26041 gacacaatca atacagcccc aagcagtgta acccatgacc tcaacgccat cttcaatcgc
    26101 tttggcaact tgcacgagat gctgactcat gtagtcaatg cgataatcat cgtgaaccgt
    26161 catgctgcca tctgcggttt gcaccagctg atcctttgca cctaagccat tttcaacaat
    26221 aaacaaagga atctggtagc ggtcatagta ctgattcaag accaatcgca gaccaattgg
    26281 gtctatctgc caaccccact ccgaagattc taaatatgga ttggacaaac cacctagaat
    26341 atttccctga ccagatgtgt aagcttcagg attgtgggct tgtgtgacac tcatgtaata
    26401 gctaaaggat agaaaatcaa ccgtatgttc cgctaataat tccttgtctc cgtcttcaaa
    26461 aacaatctca atcccatttt ctttaaaata cgacttaata tatgaaggat acttgcctct
    26521 cacatgaata tctgaaaata agagattaag attttcaaat gcatgcgcag caagtatatc
    26581 acgaggatca gaggtcattg ggtaagcagg catcgccaaa atcatacacc caactttaac
    26641 atcaggattg atttcatgcg ctagctttgt gacactagca gatgctacca attcatgatg
    26701 aatagcttgg tataaatctt gcaggctcag cttttctttt tctgtcatga tgccaccact
    26761 ggtaaagggc atgtgtaaaa tcgagttgac ttcgttgaag gtcaaccagt atttgacttt
    26821 gtccttatag cgttccatga ctgtctgagc aaagcgctca aaaaagccaa tcagacgtct
    26881 atctgtccaa ccattatagg ctttggccaa atgcagaggt gtctcataat gcgacaaggt
    26941 cactaagggt tcgataccat agttcaacaa ttcatcgaat aagtcatcat agaattgcaa
    27001 acctgcttca ttgggctggt catcgtcacc atttggaaaa atacgagacc aagcaataga
    27061 tgttcgaaag actttaaagt ccatctcggc aaaaagagca atgtcttctt tgtagcggtg
    27121 gtaaaaatca atcgcctcta aagttaaatt atcgctggta ggactatctg tccattctcc
    27181 tagtccacca tttggtagaa catcttgtac ggacaaacct tttgtgtctg cctcaaatgc
    27241 tccttccact tggttggcag ctactgcgcc gccccataag aaatcttttg gaaaaattcc
    27301 cattctccct ctccttttag gttataaaat gactaacaaa gcgtcctctt tagtcaccat
    27361 ttttttgtca tcttgaataa taatctccaa ctgctcagcc gaattggtca cgaccacagg
    27421 actgatgagt gaatagcctt ttgaagtaat gaaatcaata tccattttta gaagtgcctg
    27481 gcctcgtttc acgacatcgc caacagacac taactgctcg aaaccttgcc cttttaactc
    27541 aaccgtatca ataccgacat gaatcaagac ttccacacca cgtgtgtttt ttaaggcaac
    27601 agcatgcttg gttggaaaga aagtgatgac ttcaccatca aagggggcat agaccacacc
    27661 ttcgcttggt aatatggcaa atccttctcc taataacttg gaagaaaagg tttcatcagg
    27721 aacttctgat agaaaaagca tctcacctgt cattggagca tacaacgttt gtttggttga
    27781 caagccgctc ttaacttgtg acatatctgc tgcaacactc acttgctttg gggactcttc
    27841 atcaattccc atgaaccaag tcaaactgaa ggctaacaca atcgtcgcag ctccggcaat
    27901 caaagcattt ataaaattag ctccgcctga tgggttgatg tactgtggta aaccaataat
    27961 agaaggtacg acaaaggtat aagaagcaat attgacaaaa ccaataaagg ctccaaccag
    28021 accacctgaa atcatggctg catagagtgg ctttttaaat ttcagtgtga ccccataaag
    28081 tgccggttcc gtgatacctg ccagcaaagc tgaaatcccc gcggcaaggg caacttgacg
    28141 tgtttgtttc tgctttgttt taaaggcaac agccaaagat gcggctcctt gagccaaatt
    28201 agaagccaac attgctggta aaatcaagac atctggtgtt gcgaccgaag cggccaaaaa
    28261 gattggtgca aaagcccagt gcatccccgt catgacaaca agcggcataa tcgctcccaa
    28321 aatggcaagt gctagccacg gagccacatg ataaatagcg agcatggtat tagataaccc
    28381 ttgaccaata atcacaccaa gaggacctac aaccactaaa gccaaaaagc cagaaatcaa
    28441 aatgattaag gttggctgta agaagctttt catcacgctt ggcaccaagc gattgaccca
    28501 tttttcaatg tattgcatca agtaaaccat catcaaaatg ggaataacag atgagccata
    28561 gctagcagga gtgactggtg ctccaaataa agtcaatggt ttaccttctg ctaccattgc
    28621 aacaaaggca ggatgcaata aaatccctgc gatcgtcgct gctaaaacag gtgtcacctt
    28681 aaagcgcgcc gctgctgaat aggcaatcat gacaggcata aaataataag ccgcatcgcc
    28741 aaagaagtta ataaaggcaa cggtttgaga gtccgcactc gctaagccaa gcataggaag
    28801 caagattccc accactttga gcattccacc accaagaagg gctggaatca gcggtgtaat
    28861 cacgccagca atggtttcaa tcaagcgctc aaggatactt cctttcgctt ttgaagaatg
    28921 atcttcatct tgattgtcaa aatgccctag gcttaaaaag gcttggtaat aattattgac
    28981 atcattgccc aagataattt ggtactggcc gtttttgcgc atcacgccaa tcacatttga
    29041 aatggctttg acttgctgat ctttaacttt ctcatcattt ttcagtacta agcgtagacg
    29101 tgttacacag tgtgtcacac gttgaatatt tgttttgcca ccaaccgccg ctaaaatagc
    29161 cttggcagtt tcttgatacg tcatattaag tctcctttaa atgtttcact aaacgtttaa
    29221 catgaaccgt caaatacaaa agttcgcttg aattaaggtg gtagttgtac cgctttttta
    29281 tctcctcact aatatctaag acgcaccggt attctttagg gtatttcgtt ttcaccacta
    29341 aaagcagttc cgcatcatca tcatcgtaag acatctctga taacacacgt tgagcaaata
    29401 atttaatatg ggtcataaag cggtaataat cgatggatgt gtcatccaat tctgttttga
    29461 agtctgtttt gattttttgc tcaatataag aaacaatctc cgtcaggcga tgtggctcct
    29521 taaaaggagt gtccaaacta gcattgacaa aatgcatggc aataaaagct gcttcatcaa
    29581 tagctagagt gactcctaga ttacgctcaa ttagctctaa ggcttttacc cctagactat
    29641 attcatcagg ataatagcgt tgaatttccc accgtagtgg gttatgaatc aacattcctt
    29701 gctcatgcct ttcaatagca gagtgaatat gatccgttaa attaatatag agaatttcat
    29761 ctaaggtttt gcctaattta attttaccta gattgataat tctctctgag caagcaacaa
    29821 cttcttgtgg caccgtaata aataactctg tgaagcgatt catattgtca ctatttttta
    29881 aaacaaatga tgtttcgata gcattaagct caatactatc tccaactttt tttccaaagg
    29941 caatcccttt tcccatcaat aaaatatcta atccttgatg gttggtcgaa atcgccgcat
    30001 tatggtttaa aacgcgttta atcaacataa aagtcctagt cctttagtat cacaataaaa
    30061 aatgacctat ccaaaagctc gctaaaaagc acttttcgaa taggtccctg ctcacaaaac
    30121 tgtgataaca tcctttaata ttctgattgt tatgtctcta gcctgcttac ttgaagtaat
    30181 cacatctgag aacgctttaa tgttatcatc tctttaagat tctgtcaagc gttttcaaaa
    30241 aattctctga aagagcaact attgttattc gtatctattg atttagcaaa aaagagtttc
    30301 tgacaatttt aagattatat gatacaataa caatcgatta gataaaggag ttatcatgtc
    30361 acatcatcaa caaaccgttt caaaacaaac aattatggcg attatcgcca tagcactcat
    30421 tggtttttca ggaattttgt ctgaaaccag tatgaatgtc accttcccga cactgatgtc
    30481 agtctatcag ttacccttaa atagcttgca atggatgacg accatttatt tactagcagt
    30541 ggcgattatg atgaccactt cggctacact gaaaaaaaat gtgcgggaaa gacccctctt
    30601 ttttatggcc acaggtcttt ttacatttgg caccatcttg gccgttctga cccagtcctt
    30661 tgcgatcatg ttgctagccc gcatttttca aggcattggt actggtctgg taatgcctca
    30721 gatgtttaat attattttag agcgtgtccc aatgcataag gtaggtctat ttatgggatt
    30781 tgctggtctt attattagct tagcacctgc ttttgggcct acttatgggg gctttatgat
    30841 tagccatttt agttggcaat ggatttttat ctgtatactc cctgtaccac tgattgcagg
    30901 tattctagct tattattacc tcgaagattc tccagtaagc gaaaaagtac cctttgactg
    30961 gttggcattt attgcactat cgattagctt aacttctgcc ttattagcta ttactagtct
    31021 agaaaacgga agtgttaatt tgtattactt agggcttttt attctcagct ttgtcctctt
    31081 cctctacaag aatctcacag ctaagcaacc ctttcttgat attcgcattc tcaaaattcc
    31141 ctctctaacc tttggcctga ttcccttttt tgtcttccag ctgattaatt taggcataaa
    31201 ttttctaacg ccaaacttta ttgtcatgga aaagattgct aatagttctc aagctggtat
    31261 ggtgttacta cctggtacct tactcggagc tctactagca cctgcttttg gtaaacttta
    31321 tgatcaaaaa ggagcaaaac tttcgcttta tttaggaaat gccttattta gtttatcatt
    31381 gattattatg acacttcaaa caagacattt tatgctttta ccattcactc ttttatatat
    31441 tttattcacg tttgggcgta acatgggctt taataatagc ttagccacgg ctattcgaga
    31501 attgcctgcc gagaaaaatg ccgatgccac ggccattttt cagatgatgc agcaatttgc
    31561 tggcgctcta ggaactgcta tggcatcact gatagcaaat agtcaaccag aattcacaag
    31621 cggtgtccag tctgtctacc ttctctttac tatttttgct ctacttgatt ttatcttttt
    31681 ctttgctatg ttttaccatt tagggaaaaa aggacttgcc taagctgact tacttaaaaa
    31741 ccagcctcgt tctgttgaac aagactggtt tttttcttat aaaatacctt ctaaaagacc
    31801 tttcataaag tcttctgagt ggaattctcc aatatcatcc actttttcac caaatccgat
    31861 gaacttgact ggaatgtcta attcttgacg gatggctagg actacaccac ctttggccgt
    31921 gccatcaatt ttcgttaaaa tcaaacctgt taatggtgtt atttttgaaa attccttggc
    31981 ttggctaagc gcattttgac ctgttgacgc atctaaggcc aaaagcgttt catgaggggc
    32041 atctggtagc acgcgcttaa tgatacgacc cattttttcc agctctgcca tgagattttc
    32101 tttgttttgc aggcgaccag ccgtgtcaat caggagaata tctacacctt ttgccacagc
    32161 tttttccatc ccgtcaaaaa caacagacgc tggatcagcc ttttcagggc cagtaatgac
    32221 tggcacatct actcggcgtc cccattcgac tagctgagcc acagcccctg ctcggaaggt
    32281 atctgctgcc accaacatga cttttttacc ttcttgtttg taacggtaag ccagcttccc
    32341 gattgaagtc gttttaccaa ccccgttaac acctacaaaa agcataaccg tcaagccatc
    32401 ttgataatta atggcttcat tataacgccc gtctttttca tagatatcta ccaatttttc
    32461 cacaatcaca cgtttcaagg catctggttt tttcgcattt tccagtttgg cttcataacg
    32521 caactcctct gtcaaagttg tcgccacatg tacaccgaca tctgataaga tcagcatttc
    32581 ttccaagtct tcaaagaatt cttcatccac tcgcctgaaa ttggcaaaga aactgttcag
    32641 acgagctgag aagccagtcc gcgttttttt caagctacgc ttgtattttt cttgatctgt
    32701 ctcaggaatg gtatcgtggg ttgccttgtt ttgagaagct tctgctgagg tagaggcctg
    32761 attttcttgt ggaacttctt ctggcatcac tggaaccgtt gccgctgctt tttcttgaag
    32821 gcttttttca agagcagcct tacgacgata gtagtcttcc ataacctgtt ggctactgga
    32881 ttgctctttt gaagtgtcta ctgcttcttc attcgccgaa tcatttgagg catcagcttg
    32941 agcttgtttt gttggaaact gttccacaac tggctggtca acaaggtctt ttggttctga
    33001 tgccgtagat tgactaagtt cacttacctt aaggtctgtt ttgtcggcag caaactcatt
    33061 ggtatcttct gatggatgat tactattgtc acctgtttct tcctcaacct gtgcgtttac
    33121 tgaagctgct tcgggaccat tttccaccga acctgcgccc tctgaagaaa cgtcctcttg
    33181 ttgaaccacc tcaatcttgt tttgctcatt ggctttttca gaaagctcct ctttctgagt
    33241 tgtttctgtc aaaagatttt cttctaactt ttcctcagct accttcgggg tttctttttt
    33301 cccaaataaa cgatcaaata atcccattaa ctctcctctt ctaaaataaa tgtcttcaca
    33361 gcttctgcga ctccagactc atcattggtt aaggtggtca cggcatcggc aacggcttta
    33421 gctcctgaaa caccattagc catagctact ccaagtcctg cccattctaa catcgcaaag
    33481 tcgttagctt cgtctcccat agccatgaca tgtctagcgt caaggccaag atgctggcaa
    33541 agcaagttta atccaaaagc tttatcaatg ccttttggca tgatttcaaa aataatatct
    33601 cgtgatttaa aggcttcaaa atcctcaaat aagcttgggg atagctttat aatctgctga
    33661 tccaaaaagt ctggatcggt gactgtcaca attttgttat aggtaatgtc ttttggtagt
    33721 tccgctacag atgtcacctc aataaaggtc aacagtgggt tagctagatg gtactgcgaa
    33781 tgcctaccgt ccttacttgg aatgctatac acatccccac cactgataat atctgttggt
    33841 aagccaaccg cctctaaagc ttgctgaatc tggcaaacct ggtcaaaact gagagagctc
    33901 ttatccaaaa cctcccctgt atttcgctga accaaacccc cattaaaggt aatactgtag
    33961 tcatcgtggt caagcaagtc caactcttct agcaagttac cgatggcctt tagagggcga
    34021 cctgtggtaa taaccacctt aacccctttt tctctagccg ctgctaaggc ttttttattg
    34081 gcatctgtga caattttttc agtgttgtag agggtaccgt ctaaatcaag cgccaaaatt
    34141 ctaatattca tgagttacac tagtcctttt aaatacgtta gtactgctcc gtcattacag
    34201 tggccaatga cttggtctga aatcactttg atttcaggtc ttgcattttc cgtagcaata
    34261 gccctaccag caaactccaa catctggtaa tcattaaaat tatccccaaa agcaatcgtt
    34321 tctgctttct taatgcccaa ggcttgacaa agatgctcca taccaaagcc cttattcacc
    34381 tcttttaaga taatatcaat ggaatcaaag ccggtggtca ctgcagtggc ataagggagg
    34441 gcttgattca accaatcgct tccttctaag acggtatggc ctgtgaagtt ggtagaaact
    34501 ttaaaaatgg cgtcattctc catatcttca aaaccattaa taaccttaac attagcatag
    34561 taatgttttg ttttttgaat gtactcttct gatgcgcctt ttaaaatata ggctgccttt
    34621 tgcccagaaa aaaccatacc tgtttctaca tagtgaggat tggctaaaac cttttttgca
    34681 acttcggtat actgctcttt ggtcatcata tctgcaaata gaatctcacc atggtattga
    34741 accacggaac cattttctgc aataacagca atctgatcca aaaagggctc aaagagctga
    34801 tcaatggcta agagcgagcg gccactcgaa accgcaaata aaatgccttt ttcggctaac
    34861 tttggcaaga gcgcagctag ttgctcttga ttataagtac catcttcagc taggaaggta
    34921 ccatccatat ctgttgctat tagtttaatc atacaatgtc tccttttttt actctagggg
    34981 ggtgcaaacg ccatttaggg tgtgcacttt ttaacgattt aaataagccc catcatttct
    35041 gcattctgtt caatttttct tattatatca taaaaagcag ctttcattcc attcgtaaaa
    35101 aacagccaga ttatatttcg ataacctgac cgtttttgaa gtgaactgct acttttttat
    35161 ttttataaat gaccactgga tgtatactat aaaagtagac aaaactttgt gtgttacaat
    35221 ctgttttaaa agacacaaaa acgaaaggac aacctatgtc aacagttaaa cgctacgatg
    35281 aagaattcaa acaatctctt gtcaaccttt atcaaactgg aaaaactcag tctgaactct
    35341 gtaaagacta tggggtatcc gcttctgcgc ttgcaaaatg gatcaaacag tattctcaag
    35401 tcaaactcga agataattct gtacttactg ccaagcaaat acaagagcta caaaaacgta
    35461 atgcgcagct tgaagaggag aaccttatcc taaaaaaagc aagtgccata ttcatgcaaa
    35521 acttaaagta agactcctcg ctgtctatcg gttacgcttt gaacacgaca caacaacctt
    35581 atgtcgtgtt ttacgtgtca atcgctccac ttactataaa tttctaaaac ataagccctc
    35641 aaaaagagaa ttggataatc aaatttatag aaaacaaata cttgagattt ataccaaagc
    35701 aaacaaaaga cttggtgtga agtctatcaa ggtcattctt caaagagact acgacacaaa
    35761 aatctctgaa ggaagaattt accgtcttat gaagaatatg gcgctcccta aaatggctac
    35821 cgttaagcca aaaacagctc ttaaaaggac tcaaaaaacg tatcctcaaa acttactcaa
    35881 ccagaaattt aatcctgaca aaccaaatca agtatggtct actgacttca cctatatttc
    35941 tattggatat aagaaatatg tctatctctg cgcaatactt gatctctatt ctagaaaatg
    36001 tattgcttgg aaactgagtc atcatatgga tgcaaagtta gcatgtgaca ctctagaatt
    36061 agctcttaat aaaagaaaga ttgaaggaac acttctcttt cattccgacc aagggtcaca
    36121 atttaaggcc aggaaattga gaaaaataat tgatgacaac actatcatgc attctttttc
    36181 taaacctgga tatccttatg ataatgccgt aactgaagca tttttcaagt atttaaagca
    36241 tagacaaatc aaccgaaaaa agtatcaaaa tatcaaacag gttcaattag actgctttga
    36301 atacattgag aatttttata acaattacaa cccacatacg gctaatctag gactaacccc
    36361 taatcagaaa gaagaaaatt agtttaacgt aataaaataa cacggttttc tgtctactta
    36421 tttgacatta gtccaaaata catcctaatg gtgtcgtttt ttttatttca ctagacattc
    36481 gttctagttt aggatataag aaggtctcaa atttttttca tgatgtttat tccaaacatc
    36541 caatcacctt tctatataac tgttcattta gtggtataga caaacagaaa tactccgttt
    36601 ctagtttcat agaagacgga gtatttttta gtagagacgt ctggattgat agatagttgg
    36661 tatatagctc atccctacta ggctttcatt atagactatg gtgggtttcg ttgacaacaa
    36721 tatgtgatta ttattctaaa tgacagtata catcaactat caagtatgct tttaatttta
    36781 ttttttaaag ctacagatga aaatctgtat tccatacttc ca
//
LOCUS       DATXIG010000014        36064 bp    DNA     linear   BCT 28-DEC-2023
DEFINITION  TPA_asm: Streptococcus pyogenes strain MGAS11113
            SAMN08555799-rid19682583.denovo.014, whole genome shotgun sequence.
ACCESSION   DATXIG010000014 DATXIG010000000
VERSION     DATXIG010000014.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN08555799
            Sequence Read Archive: SRR6740495
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pyogenes
  ORGANISM  Streptococcus pyogenes
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 36064)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 36064)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-OCT-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-OCT-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001918058.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001918058.2
            Genome Coverage       :: 368x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/12/2023 11:23:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 1,806
            CDSs (total)                      :: 1,769
            Genes (coding)                    :: 1,722
            CDSs (with protein)               :: 1,722
            Genes (RNA)                       :: 37
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1, 1 (5S, 23S)
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 47
            CDSs (without protein)            :: 47
            Pseudo Genes (ambiguous residues) :: 0 of 47
            Pseudo Genes (frameshifted)       :: 30 of 47
            Pseudo Genes (incomplete)         :: 20 of 47
            Pseudo Genes (internal stop)      :: 9 of 47
            Pseudo Genes (multiple problems)  :: 11 of 47
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..36064
                     /organism="Streptococcus pyogenes"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN08555799-rid19682583.denovo.014"
                     /strain="MGAS11113"
                     /serotype="Emm28"
                     /isolation_source="normally sterile site"
                     /host="Homo sapiens"
                     /db_xref="taxon:1314"
                     /country="Finland"
                     /collection_date="2002"
                     /collected_by="National Public Health Laboratory"
     gene            2..1828
                     /gene="dnaK"
                     /locus_tag="VC122_000698"
     CDS             2..1828
                     /gene="dnaK"
                     /locus_tag="VC122_000698"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002946936.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone DnaK"
                     /protein_id="HEP5640642.1"
                     /translation="MSKIIGIDLGTTNSAVAVLEGTESKIIANPEGNRTTPSVVSFKN
                     GEIIVGDAAKRQAVTNPETVISIKSKMGTSEKVSANGKEYTPQEISAMILQYLKGYAE
                     DYLGEKVEKAVITVPAYFNDAQRQATKDAGKIAGLEVERIVNEPTAAALAYGMDKTDK
                     DEKILVFDLGGGTFDVSILELGDGVFDVLATAGDNKLGGDDFDQKIIDFLVAEFKKEN
                     GIDLSQDKMALQRLKDAAEKAKKDLSGVTQTQISLPFITAGSAGPLHLEMSLSRAKFD
                     DLTRDLVERTKTPVRQALSDAGLSLSEIDEVILVGGSTRIPAVVEAVKAETGKEPNKS
                     VNPDEVVAMGAAIQGGVITGDVKDVVLLDVTPLSLGIETMGGVFTKLIDRNTTIPTSK
                     SQVFSTAADNQPAVDIHVLQGERPMAADNKTLGRFQLTDIPAAPRGIPQIEVTFDIDK
                     NGIVSVKAKDLGTQKEQHIVIKSNDGLSEEEIDRMMKDAEANAEADAKRKEEVDLKNE
                     VDQAIFATEKTIKETEGKGFDTERDAAQSALDELKAAQESGNLDDMKAKLEALNEKAQ
                     ALAVKMYEQAVAAQQAAQGAEGAQANDSANNDDVVDGEFTEK"
     gene            2109..3245
                     /gene="dnaJ"
                     /locus_tag="VC122_000699"
     CDS             2109..3245
                     /gene="dnaJ"
                     /locus_tag="VC122_000699"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002993772.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone DnaJ"
                     /protein_id="HEP5640643.1"
                     /translation="MNNTEYYDRLGVSKDASQDDIKKAYRKMSKKYHPDINKEAGAEQ
                     KYKDVQEAYETLSDSQKRAAYDQYGAAGAQGGFGGGAGGFGGFDGGGFGGFEDIFSSF
                     FGGGGSRNPNAPRQGDDLQYRVNLSFEEAVFGVEKEVSYNREATCGTCLGSGAKPGTA
                     PVTCRKCHGSGVMTIDTQTPLGMMRRQVTCDICHGSGKEIKEPCQTCHGTGHEKQAHK
                     VSVKIPAGVETGQQIRLQGQGEAGFNGGPYGDLFVILNVLPSKQFERNGSTIYYNLDI
                     SFTQAALGDTVEIPTVHGDVEMAIPAGTQTGKTFRLKGKGAPKLRGGGQGDQHVTVNI
                     VTPTKLNDAQREALQAFAEASGEKMLHPKKKGFFDKVKDALEDI"
     gene            3465..4256
                     /locus_tag="VC122_000700"
     CDS             3465..4256
                     /locus_tag="VC122_000700"
                     /EC_number="4.2.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010922598.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="enoyl-CoA hydratase"
                     /protein_id="HEP5640644.1"
                     /translation="MQFKHIIFDVVDDLATLTLNRPEVSNGFNIPICQEILVALAEVK
                     RDTSVRFLLIKAVGKVFSVGGDLVEMQEAVAKDNVQSLVKIAELVQEISFAIKHLPKP
                     VILCADGAVAGAAFNIALAVDFCIASTQTKFIQAFVNVGLAPDAGGLFLLTRAVGLNR
                     ATHLVMTGEGITADKGLDYGFVYRTAESDKLDKVCLQLLKRLRRGSSNSYAGMKSLVW
                     QSFFTGWEDYAKAELAIQEELAFKEDFKEGVIAFGERRRPNFQGK"
     gene            4333..4767
                     /locus_tag="VC122_000701"
     CDS             4333..4767
                     /locus_tag="VC122_000701"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010922597.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family transcriptional regulator"
                     /protein_id="HEP5640645.1"
                     /translation="MEYDKINQYLVDIFNRILVIEEMSLKTSQFNDVSLKEMHTIEII
                     GKYDQVTPSDIARELMVTLGTVTTSLNKLEAKGYIARTRSRSDRRVVYLSLTKRGRLL
                     DRLHAKFHKNMVGHVIADMSDEEMQALVRGLGNLHQFLEDLV"
     gene            4768..5742
                     /locus_tag="VC122_000702"
     CDS             4768..5742
                     /locus_tag="VC122_000702"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002993767.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ketoacyl-ACP synthase III"
                     /protein_id="HEP5640646.1"
                     /translation="MIFSKISQVAHYVPQQLVTNNDLASIMDTSHEWIFSRTGIAERH
                     ISRDEMTSDLAIQVADQLLTQSGLKADAIDFIIVATISPDATMPSTAAKVQAAIAATS
                     AFAFDMTAACSGFVFALAMADKLIASGAYQNGMVIGAETLSKLVNWQDRATAVLFGDG
                     AGGVLLEASKDKHVLAETLHTDGARCQSLISGETSLSSPYSIGKKAIATIQMDGRAIF
                     DFAIRDVSKSILTLMAQSDITKDDIDYCLLHQANRRILDKIARKIDVPREKFLENMMR
                     YGNTSAASIPILLSEAVQKGQIRLDGTQKILLSGFGGGLTWGSLIVKI"
     gene            5803..6027
                     /locus_tag="VC122_000703"
     CDS             5803..6027
                     /locus_tag="VC122_000703"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002938891.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl carrier protein"
                     /protein_id="HEP5640647.1"
                     /translation="MAVFEKVQEIIVEELGKETEEVTLETTFDDLDADSLDVFQVISE
                     IEDAFDIQIETEEGLNTVGDLVAYVEEKSK"
     gene            6229..7200
                     /gene="fabK"
                     /locus_tag="VC122_000704"
     CDS             6229..7200
                     /gene="fabK"
                     /locus_tag="VC122_000704"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011888636.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="enoyl-[acyl-carrier-protein] reductase FabK"
                     /protein_id="HEP5640648.1"
                     /translation="MKTRITELLNIDYPIFQGGMAWVADGDLAGAVSNAGGLGIIGGG
                     NAPKEVVKANIDRVKAITDRPFGVNIMLLSPFADDIVDLVIEEGVKVVTTGAGNPGKY
                     MERLHQAGIIVVPVVPSVALAKRMEKLGVDAVIAEGMEAGGHIGKLTTMSLVRQVVEA
                     VSIPVIAAGGIADGHGAAAAFMLGAEAVQIGTRFVVAKESNAHQNFKDKILAAKDIDT
                     VISAQVVGHPVRSIKNKLTSAYAKAEKAFLIGQKTATDIEEMGAGSLRHAVIEGDVVN
                     GSVMAGQIAGLVRKEESCETILKDIYYGAARVIQNEAKRWQSVSIEK"
     gene            7225..8163
                     /gene="fabD"
                     /locus_tag="VC122_000705"
     CDS             7225..8163
                     /gene="fabD"
                     /locus_tag="VC122_000705"
                     /EC_number="2.3.1.39"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011018157.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ACP S-malonyltransferase"
                     /protein_id="HEP5640649.1"
                     /translation="MTKTAFLFAGQGAQKLGMARDFYDNFAIVRKTFDQASQVLGYDL
                     RRLIDSDELKLNQTSYTQPAILTSSIAIYRVLGLHHVKPDMVAGLSLGEYSALVASGA
                     LSFEDTLSLVAKRGRLMEEAAPQGSGKMVAVMNTDVQVIEEVCQIAAKHGVVAPANYN
                     TPSQIVIGGQTDAVNVAVELLKERGVKRLIPLNVSGPFHTALLEPASRLLAKELERYN
                     FSDFKIPLVGNTEANIMEKDRIPELLARQVMEPVRFYDSVATLVESGITQFIEVGPGK
                     VLTGFVKKIDKNLLCTSVENMVSLRLFLDREDIHGN"
     gene            8153..8887
                     /gene="fabG"
                     /locus_tag="VC122_000706"
     CDS             8153..8887
                     /gene="fabG"
                     /locus_tag="VC122_000706"
                     /EC_number="1.1.1.100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017771521.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-oxoacyl-[acyl-carrier-protein] reductase"
                     /protein_id="HEP5640650.1"
                     /translation="MEIKGKNIFITGSTRGIGLAMAHQFASLGANIVLNGRSAISEEL
                     VASFTDYGVTVVTISGDVSEASEAKRMVNEAIESLGSIDVLVNNAGITNDKLMLKMTE
                     EDFERVLKINLTGAFNMTQSVLKPMIKARQGAIINVSSVVGLTGNIGQANYAASKAGM
                     IGFTKSVAREVAARNICVNAIAPGFIESDMTGVLPEKMQEQILSQIPMKRIGKAQEVA
                     HLASFLVEQDYITGQVIAIDGGMTMQ"
     gene            8902..10134
                     /gene="fabF"
                     /locus_tag="VC122_000707"
     CDS             8902..10134
                     /gene="fabF"
                     /locus_tag="VC122_000707"
                     /EC_number="2.3.1.179"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012679081.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-ketoacyl-ACP synthase II"
                     /protein_id="HEP5640651.1"
                     /translation="MTFKRVVVTGYGLTSPIGHDPETFWNNLKAGQIGIGPITKFDTT
                     DYAVKNAAEIQDFPFDKYFVKKDLNRFDRYSLYALYAAKEAINHADLNIEMVDSDRFG
                     VIVASGIGGIAEIEEQVIRLHEKGPKRVKPMTLPKALPNMAAGNVAMSLKAQGVCKSI
                     NTACASSNDAIGDAFRAIKFGTQDVMIVGGSEAAITKFAIAGFQSLTALSTTEDPSRS
                     SIPFDKDRNGFIMGEGSGMLVLESLEHAQERGATILAEIVGYGNTCDAYHMTSPNPEG
                     LGARKAIHLALQEAGIEASAINYVNAHGTSTPANEKGESQAIVAVLGKDVPVSSTKSF
                     TGHLLGAAGAIEAIATIEAMRHNYVPMTAGTQALSEDIEANVIFGEGKETAINYAISN
                     TFGFGGHNAVLAFKCWEE"
     gene            10136..10636
                     /locus_tag="VC122_000708"
     CDS             10136..10636
                     /locus_tag="VC122_000708"
                     /EC_number="6.4.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011888638.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA carboxylase biotin carboxyl carrier
                     protein"
                     /protein_id="HEP5640652.1"
                     /translation="MNIQEIKDLMAQFDTSSLREFLFKTNEGELIFSKNEQHLNASTS
                     NQEHAVPVPQVQLVPNSTASEASSPASVKDVPVEEQPQAESFVAEGDIVESPLVGVAY
                     LAASPDKPPFVAVGDTVKKGQTLVIIEAMKVMNEVPAPCDGVITEILVSNEDVIEFGQ
                     GLVRIK"
     gene            10633..11055
                     /gene="fabZ"
                     /locus_tag="VC122_000709"
     CDS             10633..11055
                     /gene="fabZ"
                     /locus_tag="VC122_000709"
                     /EC_number="4.2.1.59"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002922202.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-hydroxyacyl-ACP dehydratase FabZ"
                     /protein_id="HEP5640653.1"
                     /translation="MMDIREIQAALPHRYPMLLVDRVLEVSDDHIVAIKNVTINEPFF
                     NGHFPHYPVMPGVLIMEALAQTAGVLELSKEENKGKLVFYAGMDKVKFKKQVVPGDQL
                     VMTATFIKRRGTIAVVEARAEVDGKLAASGTLTFACGQ"
     gene            11087..12451
                     /locus_tag="VC122_000710"
     CDS             11087..12451
                     /locus_tag="VC122_000710"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003056649.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA carboxylase biotin carboxylase
                     subunit"
                     /protein_id="HEP5640654.1"
                     /translation="MFKKILIANRGEIAVRIIRAARELGISTVAVYSEADKEALHTIL
                     ADEAICIGPARSKESYLNMNSVLSAAIVTGAQAIHPGFGFLSENSKFATMCEEMNIKF
                     IGPSASVMDKMGDKINARSEMIKAGVPVIPGSDGEVYNAQEALAIANKIGYPVMLKAS
                     AGGGGKGIRKVETEADLEAAFNAASQEALGAFGNGAMYLEKVIYPARHIEVQILGDAH
                     GNIIHLGERDCSLQRNNQKVLEESPSIAIGNTLRHEMGQAAVRAAEAVAYENAGTIEF
                     LLDEDSEKFYFMEMNTRIQVEHPVTEFVTGVDIVKEQIKIAAGQPLAISQEDITITGH
                     AIECRINAENTAFNFAPSPGKITDLYMPSGGVGLRVDSAVYNGYAIPPYYDSMIAKII
                     VHGSNRFDALMKMQRALVELEIEGIITNTDFQLDLISDKRVIAGDYDTSFLMETFLPH
                     YNKD"
     gene            12460..13326
                     /locus_tag="VC122_000711"
     CDS             12460..13326
                     /locus_tag="VC122_000711"
                     /EC_number="6.4.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012560896.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA carboxylase carboxyltransferase
                     subunit beta"
                     /protein_id="HEP5640655.1"
                     /translation="MALFRKKDKYIRITPNNSLKGSVSHNVPEVPDELFAKCPACKHM
                     IYKKDLGLAKICPTCSYNFRISAQERLTLTVDEGSFQELFTSIETKDPLRFPGYQEKL
                     QKAKETTGLHEAVLTGKAMVKGQQIALAIMDSHFIMASMGTVVGEKITRLFELAIEEN
                     LPVVIFTASGGARMQEGIMSLMQMAKVSAAVKRHSNAGLFYLTILTDPTTGGVTASFA
                     MEGDIILAEPQSLVGFAGRRVIETTVRENLPDDFQKAEFLQDHGFVDAIVKRTELRDK
                     IAHLVAFHGGGQ"
     gene            13323..14093
                     /locus_tag="VC122_000712"
     CDS             13323..14093
                     /locus_tag="VC122_000712"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012678443.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA carboxylase carboxyl transferase
                     subunit alpha"
                     /protein_id="HEP5640656.1"
                     /translation="MTDVSRILKEARDQGRLTTLDYANLIFDDFMELHGDRHFSDDGA
                     IVGGLAYLAGQPVTVIGIQKGKNLQDNLARNFGQPNPEGYRKALRLMKQAEKFGRPVV
                     TFINTAGAYPGVGAEERGQGEAIAKNLMEMSDLKVPIIAIIIGEGGSGGALALAVADQ
                     VWMLENTMYAVLSPEGFASILWKDGSRATEAAELMKITAGELYQMGIVDRIIPEHGYF
                     SSEIVDIIKANLIEQITSLQAKPLDQLLDERYQRFRKY"
     gene            complement(14326..15603)
                     /gene="serS"
                     /locus_tag="VC122_000713"
     CDS             complement(14326..15603)
                     /gene="serS"
                     /locus_tag="VC122_000713"
                     /EC_number="6.1.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012678442.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine--tRNA ligase"
                     /protein_id="HEP5640657.1"
                     /translation="MLDLKRIRTDFDTVAAKLKNRGVSEDTLTHLKELDEKRRALLVQ
                     SEELKAERNIASAAIAQAKRQKEDATQQIADMQKVSADIKTIDNQLVAIDQQVTDIIT
                     VLPNTPHDSVPVGADEEDNVEIRRWGTPRDFDFEVKAHWDLGEDLDILDWERGAKVTG
                     ARFLFYKNLGARLERALYNFMLDEHIKEGYQEIITPYMVNHDSMFGTGQYPKFKEDTF
                     ELADTNFVLIPTAEVPLTNYYRGEILDGKELPIYFTAMSPSFRSEAGSAGRDTRGLIR
                     LHQFHKVEMVKFAKPEESYQELEKMTANAENILQKLGLPYRVISLCTGDMGFSAAKTY
                     DLEVWIPAQNTYREISSCSNTEDFQARRAQIRYRDEADGKVKLLHTLNGSGLAVGRTV
                     AAILENYQNEDGSVTIPEVLRSYMGGETVISPK"
     gene            complement(15995..16354)
                     /locus_tag="VC122_000714"
     CDS             complement(15995..16354)
                     /locus_tag="VC122_000714"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011528899.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF956 family protein"
                     /protein_id="HEP5640658.1"
                     /translation="MAQSLNTSVEYKTKAVSYLGIGGKVGHILLGDKALEFYNDKNVN
                     DYIQIPWTAINHIGANVSRKKVSRHFEIFTDQGKFLFASGDSGKILKITRQHIGNEKV
                     ITLPTLMQTFINKFFRH"
     gene            complement(16468..17379)
                     /locus_tag="VC122_000715"
     CDS             complement(16468..17379)
                     /locus_tag="VC122_000715"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000139230.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS mannose/fructose/sorbose transporter family
                     subunit IID"
                     /protein_id="HEP5640659.1"
                     /translation="MTEQIKLTKSDRQRVWWRSQFLQGSWNYERMQNMGWAYALIPAL
                     KKLYTSPEDRAAALERHMEFFNTHPYVAAPIIGVTLALEEERANGTPIDDKAIQGVKI
                     GMMGPLAGIGDPVFWFTVRPILGALGASLASTGNIVGPLLFFFGWNLIRMAFLWYTQE
                     FGYKAGSEITKDMSGGILQDITKGASILGMFILAVLVQRWVSINFTIDLPGKQLSDGA
                     YVVFPDGAVKGAELKTILANAIGGMSLDKVQAQTLQGQLDSLIPGLAGLLLTFLCMWL
                     LKKKVSPIAIIIGLFAFGILAHLAGIM"
     gene            complement(17396..18205)
                     /locus_tag="VC122_000716"
     CDS             complement(17396..18205)
                     /locus_tag="VC122_000716"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000763694.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS mannose/fructose/sorbose transporter subunit
                     IIC"
                     /protein_id="HEP5640660.1"
                     /translation="MSDISIISAILVVIIAFFAGLEGILDQFQMHQPLVACTLIGLVT
                     GHLEAGVILGGTLQMLALGWANIGAAVAPDAALASVAAAIIMVKSGDFTQKGITFAYS
                     TAIPLAVAGLFLTMIVRTLSTALVHAGDKAATEGNFAGIERFHFIALLLQGLRIAVPA
                     ALLVAVPTSAVQSVLNAMPNWLNEGMQIGGAMVVAVGYAMVINMMATREVWPFFALGF
                     ALAAISQLTLIAMGVIGVAIAFIYLNLSKKGGNGGNAAGSADPIGDILEDY"
     gene            complement(18294..19286)
                     /gene="manX"
                     /locus_tag="VC122_000717"
     CDS             complement(18294..19286)
                     /gene="manX"
                     /locus_tag="VC122_000717"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011888640.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS mannose transporter subunit IIAB"
                     /protein_id="HEP5640661.1"
                     /translation="MGIGIIIASHGKFAEGIHQSGSMIFGEQEKVQVVTFMPNEGPDD
                     LYGHFNNAIQQFDADDEILVLADLWSGSPFNQASRVAGENPDRKMAIITGLNLPMLIQ
                     AYTERLMDAGAGVEQVAANIIKESKDGIKALPEDLNPVEETAATEKVVNALQGAIPAG
                     TVIGDGKLKINLARVDTRLLHGQVATAWTPASKADRIIVASDEVAQDDLRKQLIKQAA
                     PGGVKANVVPISKLIEASKDPRFGNTHALILFQTPQDALRAVEGGVEINELNVGSMAH
                     STGKTMVNNVLSMDKEDVATFEKLRDLGVTFDVRKVPNDSKKNLFELIQKANIK"
     gene            complement(19638..20450)
                     /locus_tag="VC122_000718"
     CDS             complement(19638..20450)
                     /locus_tag="VC122_000718"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002983364.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Cof-type HAD-IIB family hydrolase"
                     /protein_id="HEP5640662.1"
                     /translation="MTKKLIAIDLDGTLLHHDNTISTYTQKIIKAVQDKGHHVIISTG
                     RPYRMALDYYLQLNLKTPIITFNGALTHMPEQKWAYEHNVTLDKGYLLRLLKYQDDFQ
                     MDFIASEYRKNVYITMTNPESIDPQLFGVDEITQDMALEITKITRNPNALLMQTHHED
                     KYALAKNMRAFFKDEIEIDSWGGPLNILEISSKNVNKAYALNYLLGIYNMDKKDLIAF
                     GDEHNDTEMLAFAGTGYAMKNASPVLLPYADQQLNFSNEEDGVAKKLEELFL"
     gene            20714..22174
                     /locus_tag="VC122_000719"
     CDS             20714..22174
                     /locus_tag="VC122_000719"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010922585.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NCS2 family permease"
                     /protein_id="HEP5640663.1"
                     /translation="MEKFFKLSENGTTVSTEIMAGLTTFFAMSYILFVNPSILGAAGM
                     PSNAVFLATIIAAAISTLIMGLFANVPYALAPGMGLNAFFTYTVVFALGFSWQEALAM
                     VFICGLFNIFITVTKFRKSIIKAIPVSLQHAIGGGIGVFVAYLGFKNANIITFSISAE
                     NIVMVNGVEPAKASAKTFADGLLFVDANGGVVPTISSFTDSSVLLAIFGLLLTTALVI
                     RNFRGAILIGIVATTLVGIPLGIVDVSNLNFGISHIGEAWTELGTTFLAAFDGLSSLF
                     SDSSRLPLVFMTIFAFSLSDTFDTIGTFIGTGRRTGIFSQDDENALENSIGFSSKMDR
                     ALFADAIGTSIGALVGTSNTTTYVESAAGIAEGGRTGLTAVSTAVCFLLSILLLPLVG
                     IVPAAATAPALIVVGVMMVSSFLDVNWSKFADALPAFFAAFFMALCYSISYGIAAAFI
                     FYCLVKVVEGKTKDIHPIIWGATFLFIVNFIILTIL"
     gene            22328..22789
                     /gene="tsaE"
                     /locus_tag="VC122_000720"
     CDS             22328..22789
                     /gene="tsaE"
                     /locus_tag="VC122_000720"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002988647.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (adenosine(37)-N6)-threonylcarbamoyltransferase complex
                     ATPase subunit type 1 TsaE"
                     /protein_id="HEP5640664.1"
                     /translation="MFYSENEYTLKAYGETLGTYLSIGDVIVLSGDLGAGKTTLAKGI
                     AKGMGISQMIKSPTYTIVREYEGRLPLYHLDIYRVGDDPDSIDLDDFLFGNGVTVIEW
                     GELLGEGLLQDYLQITITKRDKGRQLDLLAHGERSRQLLEIMSHDSAKSRY"
     gene            22764..23288
                     /locus_tag="VC122_000721"
     CDS             22764..23288
                     /locus_tag="VC122_000721"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011888642.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HEP5640665.1"
                     /translation="MIQQSLVIEEGVPQDAKAVLSLVAAAAKETDFITGVETILEATP
                     HELSDFLSRSQTSFIDFCLLARLDEKVVGLLNLSGEVLSQGQVEADVFMLVAKTYRGY
                     GIGQLLLEIALDWAEENPYIESLKLDVQVRNTKAIYLYKKYGFRIESMRKNDIKSKNG
                     DDLDVYHMRKSVSQ"
     gene            23297..24580
                     /locus_tag="VC122_000722"
     CDS             23297..24580
                     /locus_tag="VC122_000722"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002983372.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LCP family protein"
                     /protein_id="HEP5640666.1"
                     /translation="MKIGKKIVLMFTAIVLTTVLALGVYLTSAYTFSTGELSKTFKDF
                     STSSKKSDAIKQTRAFSILLMGVDTGSSERASKWEGNSDSMILVTVNPKTKKTTMTSL
                     ERDTLTTLSGPKNNEMNGVEAKLNAAYAAGGAQMAIMTVQDLLNITIDNYVQINMQGL
                     IDLVNAVGGITVTNEFDFPISIAENEPEYQATVAPGTHKINGEQALVYARMRYDDPEG
                     DYGRQKRQREVIQKVLKKILALDSISSYRKILSAVSSNMQTNIEISSRTIPSLLGYRD
                     ALRTIKTYQLKGEDATLSDGGSYQIVTSNHLLEIQNRIRTELGLHKVSQLKTNATVYE
                     NLYGSTKSQTVNNNYDSSGQAPSYSDSHSSYANYSSGVDTGSSYQSASTDQDSTASSH
                     TPTTPSSSSDALAADESSSSGSGSLVPPANINPQT"
     gene            complement(24691..25047)
                     /locus_tag="VC122_000723"
     CDS             complement(24691..25047)
                     /locus_tag="VC122_000723"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002983373.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chemotaxis protein"
                     /protein_id="HEP5640667.1"
                     /translation="MKVKTVIAVGLLSFTAYKAYQKRCTIKELLSISRQAKDAAQLDL
                     DNIKANLDLIHSQGKVIQNISQDLAHKWRYFNQETQAHLTEIQNRMAKYQEDSELISS
                     ESPTCQTVILTNNHSR"
     gene            complement(25044..25463)
                     /locus_tag="VC122_000724"
     CDS             complement(25044..25463)
                     /locus_tag="VC122_000724"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011285081.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HIT family protein"
                     /protein_id="HEP5640668.1"
                     /translation="MENCIFCSIIQGDIPSSKVYEDEQVLAFLDISQTTKGHTLVIPK
                     QHVRNLLEMTAETASHLFARIPKIARAIQLATGATAMNIINNNEALAGQTVFHAHVHL
                     VPRYNEKDGISIQYTTHEPNFPVLEKLARQINQEVSS"
     gene            25535..26260
                     /locus_tag="VC122_000725"
     CDS             25535..26260
                     /locus_tag="VC122_000725"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011888644.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEP5640669.1"
                     /translation="MLNIKNLTGGYHNIPVLNDVSFSVDNGELVGLIGLNGAGKSTTI
                     NEIIGFLKPYQGSISIDGLTLAENAVAYRQKIGFIPETPSLYEELTLSEHINTVAMAY
                     DIDLEVAQKRAQPFLEMFRLTDKLEWFPVNFSKGMKQKVMIICAFVIDPSLFILDEPF
                     LGLDPLAISDLIQTLEVEKAKGKSILMSTHVLDSAERMCDRFVILHHGQVRAQGTLAD
                     LQEAFGDRSASLNDIYLALTKED"
     gene            26263..27297
                     /locus_tag="VC122_000726"
     CDS             26263..27297
                     /locus_tag="VC122_000726"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002994331.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEP5640670.1"
                     /translation="MKALFLKRRQDFQKQQNKYLRYVLNDHFVLVLMFLLGYAMVQYG
                     QLLNHFPTNHLPIQVCLGILIPLLLSMGSIATYLEEADQHFLLPKEEEVISYIKQAER
                     LSFLLWGTLQTAILLFLYPIFRRLGLSLFIFIILVLILLALKRVVLSRKTRYFLRGNR
                     LDWAKAVAFESNRKQSILKFYSLFTTVKGISTKVKERTYLNPLLKLVKQTPSNLWLSL
                     YARAFLRSSDYLGLFLRLMLLSSLSVFFIHNLYLSVSLALIFNYLVVFQLLSLYYHYD
                     YHYMTSLYPENSRSKKKNVLSFLRGLSFLMLIVNMLCCSSAPKALILIVGMVFIACIY
                     LPYKLKKIID"
     gene            27361..28152
                     /locus_tag="VC122_000727"
     CDS             27361..28152
                     /locus_tag="VC122_000727"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002983385.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphotransferase family protein"
                     /protein_id="HEP5640671.1"
                     /translation="MTTTEQELTLTPLRGKSGKAYKGTYPNGECVFIKLNTTPILPAL
                     AKEQIAPQLLWAKRMGNGDMMSAQEWLNGRTLTKEDMNSKQIIHILLRLHKSKKLVNQ
                     LLQLNYKIENPYDLLVDFEQNAPLQIQQNSYLQAIVKELKRSLPEFKSEVATIVHGDI
                     KHSNWVITTSGMIFLVDWDSVRLTDRMYDVAYLLSHYIPRSRWSEWLSYYGYKNNDKV
                     MQKIIWYGQFSHLTQILKCFDKRDMEHVNQEIYALRKFREIFRKK"
     gene            28152..28787
                     /gene="trmB"
                     /locus_tag="VC122_000728"
     CDS             28152..28787
                     /gene="trmB"
                     /locus_tag="VC122_000728"
                     /EC_number="2.1.1.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012679098.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (guanosine(46)-N7)-methyltransferase TrmB"
                     /protein_id="HEP5640672.1"
                     /translation="MRVRKRKGAEEHLANNPHYVILNPEDAKGRWHDVFGNDRPIHIE
                     VGSGKGGFITGMALKNPDINYIGIDIQLSVLSYALDKVLASEVPNVKLLRVDGSSLTN
                     YFEDGEVDMMYLNFSDPWPKTKHEKRRLTYKDFLDTYKRILPEHGEIHFKTDNRGLFE
                     YSLASFSQYGMTLRQIWLDLHASNYEGNVMTEYEEKFSNKGQVIYRVEANF"
     gene            28852..28938
                     /locus_tag="VC122_000729"
     tRNA            28852..28938
                     /locus_tag="VC122_000729"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:28886..28888,aa:Ser,seq:gga)
     gene            29046..29582
                     /gene="rimP"
                     /locus_tag="VC122_000730"
     CDS             29046..29582
                     /gene="rimP"
                     /locus_tag="VC122_000730"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002988815.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome maturation factor RimP"
                     /protein_id="HEP5640673.1"
                     /translation="MDSQGPIILEKSIKIEEVIKIANTSIIDIVTKTVTPEIKAPYEL
                     VDVEYDKMGSDYILSILVDKEGGITVEDTSDLTNIISPLLDTIDPDPFPNQYMLEVSS
                     PGLERPLKTADSLKAAVGSYINVSLYQAIDKVKVFQGDLLAFDGETLTIDYLDKTRHK
                     IVNIPYQAVAKVRMAVKL"
     gene            29757..30914
                     /gene="nusA"
                     /locus_tag="VC122_000731"
     CDS             29757..30914
                     /gene="nusA"
                     /locus_tag="VC122_000731"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002994325.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription termination/antitermination
                     protein NusA"
                     /protein_id="HEP5640674.1"
                     /translation="MSKEMLEAFRILEEEKHIDKADIIDAVTESLKSAYKRRYGQSES
                     CVIEFNEKTADFQVFTVREVVEEVFDSRLEISLKDALAISSAYELGDKIRFEESVNEF
                     GRVAAQSAKQTIMEKMRRQMREVMFNEYKEHEGEIMTGTVERFDQRFIYVNLGSLEAQ
                     LSHQDQIPGETFKSHDRIDVYVYKVENNPKGVNVFVSRSHPEFIKRIMEQEIPEVFDG
                     TVEIMSVSREAGDRTKVAVRSHNPNVDAIGTIVGRGGSNIKKVISKFHPKRVDAKTGL
                     EIPVEENIDVIQWVDDPAEFIYNAIAPAEVDMVLFDDEDLKRATVVVPDSKLSLAIGR
                     RGQNVRLAAHLTGYRIDIKSASEYDRLEAEKEAATVVEEPVVDDVMTAEEV"
     gene            30930..31226
                     /locus_tag="VC122_000732"
     CDS             30930..31226
                     /locus_tag="VC122_000732"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006531117.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YlxR family protein"
                     /protein_id="HEP5640675.1"
                     /translation="MSKVKKIPLRKSLVSGEIIAKRDLLRIVKTKDGQVFIDPTGKQN
                     GRGAYIKLDNQEALMAKKKQVFNRSFSMDIPESFYDDLIAYVDHKIKRRELGLD"
     gene            31219..31521
                     /locus_tag="VC122_000733"
     CDS             31219..31521
                     /locus_tag="VC122_000733"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003052320.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YlxQ-related RNA-binding protein"
                     /protein_id="HEP5640676.1"
                     /translation="MTNLERLSSLIGLAQRAGKVISGEELVVKAIQHQQVILVFLAND
                     AGPNVTKKVTDKSNYYNVEVSTVLNALELSAALGKPRKVAAVADAGFSKKMRTLME"
     gene            31541..34402
                     /gene="infB"
                     /locus_tag="VC122_000734"
     CDS             31541..34402
                     /gene="infB"
                     /locus_tag="VC122_000734"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014635652.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="translation initiation factor IF-2"
                     /protein_id="HEP5640677.1"
                     /translation="MSKKRLHEIAKEIGKSSKEVVEHAKYLGLDVKSHASSVEEADAK
                     KIISSFSKASKPDVTASQTVKPKEVAQPSVTVVKETGSEHVEKTQVSKPKSRNFKAER
                     EARAKEQAARKQANGSSHRSQERRGGYRQPNNHQTNEQGDKRITHRSQGDTNDKRIER
                     KASNVSPRHDNHQLVGDRNRSFAKENHKNGRFTNQKKQGRQEPQSKSPKIDFKARAAA
                     LKAEQNAEYSRQSETRFRAQQEAKRLAELARQEAKEAALKAQAEEMSHREAALKSIEE
                     AETKLKSSNISAKSTADNRRKKQARPEKNRELTHHSQEGQKKNKKSWNSQNQVRNQKN
                     SNWNKNKKTKKGKNVKNTNTAPKPVTERKFHELPKEFEYTEGMTVAEIAKRIKREPAE
                     IVKKLFMMGVMATQNQSLDGDTIELLMVDYGIEAKAKVEVDDADIERFFEDENYLNPE
                     NIVERAPVVTIMGHVDHGKTTLLDTLRNSRVATGEAGGITQHIGAYQIEEAGKKITFL
                     DTPGHAAFTSMRARGASVTDITILIVAADDGVMPQTIEAINHSKAAGVPIIVAINKID
                     KPGANPERVIAELAEYGIISTAWGGECEFVEISAKFNKNIDELLETVLLVAEVEELKA
                     DPTVRAIGTVIEARLDKGKGAIATLLVQQGTLHVQDPIVVGNTFGRVRAMVNDLGRRV
                     KSAEPSTPVSITGLNETPMAGDHFAVYADEKAARAAGEERSKRALLKQRQNTQRVSLD
                     NLFDTLKAGEIKTVNVIIKADVQGSVEALAASLVKIEVEGVRVNVVHSAVGAINESDV
                     TLAEASNAVIIGFNVRPTPQARQQADTDDVEIRLHSIIYKVIEEVEEAMKGKLDPVYQ
                     EKILGEAIIRETFKVSKVGTIGGFMVINGKVTRDSSVRVIRDSVVIFDGKLASLKHYK
                     DDVKEVGNAQEGGLMIENFNDLKVDDTIEAYIMEEIVRK"
     gene            34608..34958
                     /gene="rbfA"
                     /locus_tag="VC122_000735"
     CDS             34608..34958
                     /gene="rbfA"
                     /locus_tag="VC122_000735"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012560889.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosome-binding factor RbfA"
                     /protein_id="HEP5640678.1"
                     /translation="MANHRIDRVGMEIKREVNDILQKKVRDPRVQGVTITEVQMQGDL
                     SLAKVYYTIMSDLASDNQKAQTGLEKATGTIKRELGKQLTMYKIPDLVFEKDNSIAYG
                     NKIDQLLRELDKKD"
     gene            complement(35011..36012)
                     /locus_tag="VC122_000736"
     CDS             complement(35011..36012)
                     /locus_tag="VC122_000736"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011528895.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HEP5640679.1"
                     /translation="MKKTLKSTLILLGLTLAIGASTQVDASSRSWKSWFIEKYFWLKG
                     DKGYYSEQDETKFDQYVNSAGEASNKGFDLKEEQVNGTLVTKTVDNMQVHTWNENPSN
                     KANQKVIFYLHGGSYLNNPTTYHIDMLKTLSTSLDAKIILPIYPKAPTYSYSDAMPKL
                     VDLYRDTLKETTAQNITIMGDSAGGGLALGLALALKNESLKQPKDIVLLSPWLDVTMS
                     NPDIESYESNDPILSAWGLRKVGEIWARRDPNNTKHPYVSPINGDVSGLAPITMFVGT
                     HEIFYPDVKKFDDILTAKGHQHELIVADKMNHVYPIYPIEEAKTAQYQIIDTIQKAY"
BASE COUNT        11180 a         6322 c         7781 g        10781 t
ORIGIN      
        1 catgtctaaa attattggta ttgacttagg tacaacaaac tcagcagtag cagttcttga
       61 agggactgaa tcaaaaatca ttgctaaccc agaaggcaat cgtacaactc cttcagtagt
      121 atcattcaaa aatggtgaaa ttatcgtggg tgatgctgca aaacgtcaag cagtgacaaa
      181 cccagaaaca gtaatctcta ttaaatctaa aatgggaact tctgaaaaag tttctgcaaa
      241 tggtaaagaa tatactcctc aagaaatttc agcaatgatt cttcaatacc ttaaaggtta
      301 tgctgaagac tatcttggag aaaaagtaga aaaagcagtt attactgttc cagcttactt
      361 caacgatgca caacgtcagg caactaaaga cgctggtaaa attgcaggtc ttgaagtaga
      421 acgtatcgtt aacgaaccaa cagcagccgc acttgcttat ggtatggaca agactgacaa
      481 ggatgaaaaa atcttagttt ttgaccttgg tggtggtaca tttgacgtat caatccttga
      541 attaggtgat ggtgtcttcg acgttcttgc aacagcaggt gataacaaac ttggtggtga
      601 cgactttgac caaaaaatta ttgatttctt agtggctgaa tttaagaaag aaaatggcat
      661 tgacttatca caagataaga tggcacttca acgcttgaaa gatgctgctg aaaaagctaa
      721 aaaagatctt tcaggtgtga cacaaacaca aatttcatta ccgttcatca ctgctggttc
      781 tgccggtcct cttcacttgg agatgagctt atctcgtgct aaatttgacg atctcactcg
      841 cgaccttgta gaacgtacga aaactccagt tcgtcaagcc ctttcagatg caggattgtc
      901 attgtcagaa attgatgaag ttatccttgt tggtggatca actcgtatcc cagcagttgt
      961 cgaagctgta aaagctgaaa ctggtaaaga accaaataaa tctgtaaacc ctgatgaagt
     1021 ggttgctatg ggtgctgcta tccaaggtgg tgttatcact ggggatgtga aagacgttgt
     1081 ccttcttgac gtaacaccat tgtcacttgg tattgaaaca atgggtggtg tcttcaccaa
     1141 attgatcgac cgcaacacca ctatcccaac atctaaatca caagtcttct caacagcagc
     1201 agacaaccaa ccagctgttg atatccatgt tcttcaaggt gaacgcccaa tggcagcaga
     1261 taacaagact cttggtcgct tccaattgac tgatatccca gctgcacctc gtggaatccc
     1321 acaaattgaa gtaacatttg atatcgataa aaacggtatt gtttctgtaa aagctaaaga
     1381 ccttggtacg caaaaggaac aacacatcgt tatcaaatca aacgacggac tttctgaaga
     1441 agaaattgat cgcatgatga aagacgctga agctaatgcc gaagccgatg cgaaacgtaa
     1501 agaagaagtt gaccttaaaa acgaagttga ccaagctatc tttgctactg aaaaaacaat
     1561 caaagaaact gaaggtaaag gctttgatac agaacgcgat gcagcgcaat cagctcttga
     1621 cgagttaaaa gctgcgcaag aatctggcaa ccttgacgac atgaaagcta aacttgaagc
     1681 attaaatgaa aaagcgcaag ctttggctgt taaaatgtac gagcaagctg tagcagctca
     1741 acaagcagca caaggtgcag aaggtgcaca agctaatgat tcagcaaata atgatgatgt
     1801 tgtagatggc gaatttacag aaaagtaatg atttagttat ctagtaacat taatatccga
     1861 attcagaggt tgtaccaaac ctctgttttt ggctaaataa aatgtaaaaa tgctgacgtc
     1921 aaaatatttt aagaaaggaa tacaagttcg attattcgaa cacaggctaa agcgtgtaaa
     1981 gactcttttg cggattcgaa cacgcctacg actctgcgaa aaaagagaaa tacctcctag
     2041 atacgagagt atctttgtcg gatttcctat ttttcattga gtcgttttac ggcttggtat
     2101 cttacattat gaacaataca gaatattacg atcgtctggg ggtttctaag gacgcttctc
     2161 aggacgatat caaaaaagct tatcgaaaaa tgtctaagaa atatcatcca gatatcaata
     2221 aagaagctgg agcggagcaa aagtataagg atgttcaaga agcttacgaa accttaagtg
     2281 attcacaaaa gcgtgctgct tatgatcaat acggcgctgc aggtgctcaa ggcggctttg
     2341 gtggcggagc aggcggcttt ggtggttttg acggaggtgg ctttggtggt tttgaagata
     2401 tcttctcaag cttctttggt ggcggtggaa gccgaaaccc aaatgcccct cgtcaaggtg
     2461 acgatttgca atatcgtgtt aatttatctt ttgaagaggc tgtatttggt gttgaaaaag
     2521 aagttagcta taatcgcgaa gcaacctgcg gcacttgttt aggctcaggt gcaaaacctg
     2581 gtaccgctcc agtaacttgt cgtaagtgcc atggttcagg tgtaatgaca attgacaccc
     2641 aaacaccact tggtatgatg cgtcgacaag tcacctgcga tatttgtcat ggtagtggta
     2701 aggaaattaa agaaccctgt caaacctgtc acggtactgg tcatgaaaag caagctcata
     2761 aagtgtctgt taagattcca gctggtgtag agactggtca gcaaatccgt ttacaaggtc
     2821 aaggagaagc tggctttaat ggcggacctt atggtgattt atttgttatt ttgaatgtct
     2881 taccaagtaa gcaatttgaa cgcaatggtt ccactattta ctacaactta gatatttctt
     2941 ttactcaagc agccttagga gatactgttg aaattccaac ggttcatggc gatgttgaga
     3001 tggctatccc tgctgggaca caaacaggta aaaccttccg cctcaaaggc aaaggagcac
     3061 caaaactccg tggtggaggt caaggggatc aacatgttac cgttaatatt gtgaccccaa
     3121 ccaaactcaa tgacgctcaa agggaagcct tgcaagcatt tgcagaagcg agtggtgaaa
     3181 aaatgttaca tccaaagaaa aaaggttttt ttgataaagt gaaagatgcc ttagaggata
     3241 tttaaagatg tcaaacgtat aatctcagga taattttccc gaagttatac gtttttttac
     3301 gtcaaaacag ttagagaaaa cgttttcttt tatagatatc gtttttttct cttgtcaaaa
     3361 tggtttattt agtatatcat aattaggtaa ttgatttgat tatcaaatga ttttaaagag
     3421 aaataatttc ttagtcaaat aaattatcag aaaagaggat tgttatgcag tttaaacaca
     3481 tcatctttga tgttgtagat gatcttgcga cattgacttt aaaccgtcca gaggtttcaa
     3541 acggttttaa tattccaatt tgtcaagaga ttctggtagc attagcagaa gttaaacgcg
     3601 atacatctgt gcgtttttta ttaattaaag ccgttggaaa agtgttttca gttggtggtg
     3661 acctagttga aatgcaagaa gctgttgcaa aagataatgt gcagtcgcta gtcaagattg
     3721 ctgaactggt acaagaaatt tcttttgcaa tcaagcattt gccaaaacca gtcatccttt
     3781 gcgccgatgg agctgtagct ggagcggctt ttaatatagc tttagctgtt gacttttgta
     3841 tcgctagtac tcaaacaaaa tttatccaag cttttgtaaa tgttggacta gcaccggatg
     3901 ccggtggttt atttctattg actcgtgcag taggacttaa tcgtgcaact caccttgtga
     3961 tgacaggtga aggaattact gctgataaag gtctcgatta tgggtttgtc taccgtacgg
     4021 cagaatctga taaactagac aaagtttgtc ttcaactttt aaagcgattg agacgtggct
     4081 cttcaaattc ttatgctgga atgaaatcat tggtctggca aagctttttc acaggatggg
     4141 aagattatgc taaggcagag cttgctattc aagaggagct agctttcaag gaagatttta
     4201 aagaaggtgt catagctttt ggagaaaggc gccgaccaaa ttttcaaggt aagtaagaca
     4261 gctgttgctt gcaaaatact ttgacctttg atataatttg attgtcaaag tttattgcta
     4321 ggaggtgttt acttggaata tgacaaaatt aaccaatact tagttgatat tttcaaccgg
     4381 attttggtta ttgaagaaat gagcttaaag acgagtcagt ttaatgatgt ctctttaaaa
     4441 gagatgcaca ctattgaaat tatcggcaaa tatgatcagg tgactccaag tgatatcgcc
     4501 agagaattaa tggtaacgtt agggacagta acgactagct taaataaatt agaagcaaaa
     4561 ggttatatcg caagaacgcg atcacgctca gataggcgag ttgtttacct gtcattaaca
     4621 aaaaggggtc gacttttgga ccgcctccat gcaaagttcc ataaaaatat ggttggacat
     4681 gtcattgctg atatgagtga tgaagagatg caggcattgg tgcgtggact agggaatcta
     4741 catcaatttt tggaggattt ggtttaaatg attttttcta aaatcagtca ggtagcccac
     4801 tatgttccac agcagttagt gactaataat gatttagcta gcataatgga cactagtcat
     4861 gagtggatct tttctcgaac aggaatagca gaacgccata tttctagaga tgaaatgact
     4921 agtgacctag ctattcaggt tgctgatcag cttttaactc agtctgggtt aaaagctgat
     4981 gctattgatt ttattattgt tgcaactatc agccctgatg ccactatgcc atcaacagct
     5041 gcgaaagttc aagcagctat tgcggctaca agtgcttttg cttttgatat gactgctgct
     5101 tgtagtggct ttgtctttgc cttggctatg gcagataagc ttattgcatc aggtgcctac
     5161 caaaacggaa tggtaatagg tgcagagact ttatctaagt tagtaaactg gcaggatcga
     5221 gcgacagccg ttctttttgg agacggagct ggtggcgtgc tactggaagc cagtaaagat
     5281 aaacatgttt tagcggaaac attacacaca gatggtgcga gatgtcagag tttaatttca
     5341 ggagaaacta gtctttcatc tccgtactca attggcaaaa aagccatagc gactattcag
     5401 atggatggac gtgccatatt tgattttgcg attagggatg tgtcaaaaag catcctcaca
     5461 ctaatggcgc aatcagatat tacaaaagat gacatagact attgtttatt gcatcaagct
     5521 aatcgtcgta tacttgataa aatagcacgc aagattgatg ttcctcggga aaaattcctt
     5581 gaaaacatga tgcgttatgg taatactagt gctgctagta tcccaattct cttgtcagag
     5641 gctgttcaaa agggacagat taggttagat ggtacccaaa aaatcttact ttccggtttt
     5701 ggtggaggtt tgacatgggg aagtctaatt gttaaaatct agttattcat gtgcctagca
     5761 catttataaa aacaaattta tattttttaa ggagaaataa acatggcagt atttgaaaaa
     5821 gtacaagaaa ttattgttga agaacttggt aaggagacag aagaagtaac gctagaaact
     5881 acatttgatg atttagatgc agattcactt gatgttttcc aagttatttc agaaattgaa
     5941 gatgcttttg atattcaaat tgaaacagaa gaaggattaa atactgttgg tgaccttgtt
     6001 gcttacgttg aggaaaagtc aaaataagga taaaagtgac aagtaacatc agaagtctat
     6061 gaaaagctta ctgctgttat gttcatgttc atcactaaaa aaaacaataa gtattaatca
     6121 aaccattagg gtgggattag actcttttta ggatctagct cgctctttct tcttactata
     6181 atagtttgat tatcaaatta ttctccttat tttttataga aagttattat gaaaacacgt
     6241 attacagaat tacttaatat tgattacccc atttttcaag gaggaatggc ttgggttgct
     6301 gatggtgatt tagcaggtgc agtttctaat gctggtggtt taggcattat aggtggtggc
     6361 aatgctccca aagaagtcgt taaagctaat attgatcgtg tcaaagctat tactgataga
     6421 ccttttgggg ttaatatcat gcttttatct ccttttgctg atgatatcgt tgatctggtc
     6481 attgaagaag gtgttaaagt agtaacaaca ggcgcaggaa atccaggaaa gtatatggaa
     6541 agactgcacc aggcgggtat aatcgttgtt cctgttgtcc caagcgttgc gctagccaaa
     6601 cgtatggaaa agcttggggt agatgcagtg attgctgagg gtatggaagc tggaggacat
     6661 attggcaagt taacgactat gtctttagta agacaagttg ttgaagcggt ttcgattcct
     6721 gtcattgcgg caggtggtat agctgatggt catggtgcag cagcagcatt tatgttagga
     6781 gcagaggctg ttcaaattgg aactcgcttt gttgttgcta aagaatccaa tgcccaccaa
     6841 aattttaaag ataaaatctt agcagcaaaa gatattgata cggtgatttc tgcgcaggtt
     6901 gtgggccacc ctgtccgctc tattaaaaat aaattgacct cagcttacgc taaagcagaa
     6961 aaagcatttt taattggtca aaaaacagct actgatattg aagaaatggg agcaggatcg
     7021 cttcgacatg ctgttattga aggcgatgta gtcaatggat ctgttatggc tggccaaatt
     7081 gcagggcttg tgagaaaaga agaaagctgt gaaacgattt taaaagatat ttattatggt
     7141 gcagctcgtg ttattcaaaa tgaagctaag cgctggcaat ctgtttcaat agaaaagtag
     7201 ataaacacat gaaggataat gataatgaca aagacagcct ttttatttgc cggtcaaggt
     7261 gcccaaaaat tagggatggc aagggatttt tatgataact ttgctattgt aagaaaaacc
     7321 tttgatcaag ctagtcaagt attgggatac gatttgcgcc gtttgattga tagtgacgag
     7381 ttaaaactta atcaaactag ctatacgcaa ccagctattt tgacatcatc aattgctatt
     7441 taccgtgttc tgggcttaca tcacgttaaa ccggatatgg tagctggtct ttccttagga
     7501 gagtactcag ctttggtagc atcaggggca ctctcttttg aagataccct atccttagta
     7561 gctaagagag gccgcttgat ggaggaagca gcaccacaag gatctggaaa gatggttgcc
     7621 gttatgaata cagacgtgca agtcatcgaa gaagtctgtc aaatagctgc taaacatgga
     7681 gtggttgctc cagcaaacta taatactcct agtcaaattg taattggtgg tcagacagat
     7741 gctgtgaacg tggcagttga acttttaaag gaaaggggag ttaagcgttt aatcccttta
     7801 aacgtgtcag gtcctttcca cactgctttg ttagaaccag ctagccgctt gttggctaaa
     7861 gagttggaaa gatacaactt cagtgacttc aagattcctt tggttggtaa taccgaagct
     7921 aatattatgg aaaaagatcg tatcccagaa ctattagccc gtcaagtcat ggagcctgtt
     7981 cgtttttatg acagtgttgc gactttagta gaaagtggca taacacaatt cattgaggta
     8041 ggaccaggta aggttttgac aggttttgtg aagaaaattg ataaaaattt actatgtact
     8101 agtgttgaaa acatggtaag cttacgttta tttttagata gagaggatat tcatggaaat
     8161 taaaggaaaa aatatattta tcacaggctc aacgagaggt atcggtttag caatggctca
     8221 tcaattcgcc agtttaggag ctaatattgt cttaaatggt cgctctgcta tctctgagga
     8281 gcttgttgct agttttactg actatggtgt tacagttgtt accatctctg gggatgtttc
     8341 agaagcatca gaagctaaac gtatggtcaa tgaagctatc gaaagtttag gttcgattga
     8401 tgttttagtt aacaatgctg gcattactaa tgataaattg atgcttaaaa tgacggaaga
     8461 ggattttgag cgcgtcttga aaatcaattt gaccggtgct tttaacatga ctcaatcagt
     8521 gttaaaacca atgatcaagg cccgccaagg agcaattatt aatgtctcta gtgtggttgg
     8581 tttaacaggc aatattgggc aagcaaacta tgctgcttct aaggcaggaa tgattggttt
     8641 taccaagtct gttgcccgtg aagttgctgc tcgtaacatt tgcgtgaatg ctattgctcc
     8701 aggatttatc gagtccgaca tgacaggtgt ccttcctgaa aaaatgcaag agcaaatctt
     8761 aagtcaaatc ccaatgaaac gtattggaaa agctcaagaa gttgctcact tagcaagctt
     8821 cttggtggaa caagattata ttacgggaca agttatcgct attgatggcg gtatgacaat
     8881 gcaataagga gacgtagtga aatgacattt aaaagagtag tggtaacagg ttatggtcta
     8941 acatctccaa tcgggcatga tcctgaaacg ttttggaaca atctgaaagc tggccaaatt
     9001 ggtattggtc ctattactaa atttgacacg actgattatg cagtcaagaa tgctgctgaa
     9061 attcaggatt ttccatttga caaatacttt gtgaaaaaag atttgaaccg tttcgataga
     9121 tattctcttt atgctcttta cgcagcgaaa gaggctatta atcatgctga tttaaatatt
     9181 gagatggttg attccgatcg atttggcgtt attgttgctt caggtattgg tggaattgcg
     9241 gagattgaag agcaggtgat tcgcttacat gaaaaagggc caaaacgtgt gaagccaatg
     9301 acgttgccaa aagcgctacc aaatatggct gcaggaaatg tcgccatgtc tttaaaagct
     9361 caaggagtat gtaaatcaat taatactgcc tgcgcttctt caaatgatgc gattggggat
     9421 gcctttcgtg ccattaaatt tgggactcaa gatgtgatga tagttggtgg gtcagaggcg
     9481 gccataacca agtttgccat tgctggcttc caaagtttaa cagctctctc aacaactgaa
     9541 gatccaagca gaagctctat tccatttgat aaagaccgaa atggttttat catgggagaa
     9601 ggatcaggaa tgcttgtttt agaaagccta gaacatgcgc aagaacgcgg ggcgacaatc
     9661 ttagcagaaa ttgtgggtta tggaaatacc tgcgatgcct accatatgac atctccaaat
     9721 cctgagggat taggagctcg taaagccatc cacttagcct tacaagaagc tggtattgaa
     9781 gccagtgcta ttaattatgt caatgcgcac ggaacttcaa ctccagctaa tgaaaaagga
     9841 gagagtcaag caattgtagc cgttcttggt aaggatgttc cggtgtcttc aacaaaatcg
     9901 tttacgggtc accttcttgg agcagcaggc gctattgaag ctattgccac tatcgaggct
     9961 atgcgtcata actatgttcc gatgacggct gggacacaag ctttatcaga ggatatcgaa
    10021 gcaaatgtca tctttggaga aggaaaagaa acagctatca actatgccat ttcaaatact
    10081 tttggatttg gtggtcataa tgctgtatta gctttcaaat gttgggagga gtaggttgaa
    10141 tattcaagaa atcaaagatt tgatggctca gtttgatacc tcaagcctac gtgaattttt
    10201 attcaaaaca aatgaaggtg agctaatctt tagtaaaaac gaacaacacc tcaatgcatc
    10261 gacatcaaat caagaacatg ctgtgcctgt accacaggta caacttgttc cgaattcaac
    10321 tgcttcggaa gcgtccagtc cagcatcagt taaggatgtt cctgtagagg agcaaccaca
    10381 agcagaatct tttgtagcag aaggtgatat tgttgaaagt cctttagtcg gtgtagccta
    10441 tcttgcagct agccctgaca agccaccatt tgtggcagtc ggagatactg taaaaaaagg
    10501 acaaaccctt gtgattatcg aagccatgaa ggtgatgaac gaagtgccag cgccatgtga
    10561 tggtgtgatt acagaaattt tggtatcaaa tgaagatgtc attgaatttg gacaaggatt
    10621 ggtgcggatc aaatgatgga tattagagaa attcaagcgg ctttgcctca tcgctaccca
    10681 atgttattgg ttgatcgtgt gttagaagtg tcggacgacc atatcgttgc cattaaaaat
    10741 gtgactatta atgaaccttt ctttaacggg cattttcctc actatcctgt gatgccaggt
    10801 gttctgatta tggaagcttt ggctcaaaca gctggtgtct tagaattgtc aaaagaagaa
    10861 aataaaggca agttggtctt ttacgctgga atggataaag tgaagttcaa aaaacaagtc
    10921 gtccctggtg atcaacttgt gatgacagca acctttatca agcgtcgcgg taccattgct
    10981 gtcgttgaag ccagggcaga agttgatggc aagctagcag caagtggaac attaaccttt
    11041 gcctgtgggc aatgagaatg attgttctgc ggaggaagaa acccttatgt ttaaaaaaat
    11101 cttaattgct aatcgtggtg aaattgcagt gcgtatcatt cgtgctgcac gagaattagg
    11161 gatttccaca gttgcagttt attcggaagc ggataaggaa gctctgcaca cgatattggc
    11221 ggacgaggct atctgtattg gtcctgcccg ttccaaggaa tcctatctta atatgaattc
    11281 cgtcttgtca gcagctattg tgacaggagc ccaagcgatt cacccagggt ttggcttttt
    11341 aagtgaaaac tcaaagtttg cgaccatgtg tgaagagatg aatattaaat ttatcggtcc
    11401 atccgcatcg gttatggata aaatgggtga taaaatcaat gctcgttctg aaatgattaa
    11461 ggcaggagtg cccgttattc cagggtcaga tggtgaagtt tataacgctc aagaagcctt
    11521 agctattgcc aataagattg gttatcctgt catgctaaaa gcatcggcag gtggtggagg
    11581 taaagggatt cgtaaagtgg agacagaagc agatttggag gcggctttca atgctgccag
    11641 tcaagaagct ttgggagctt tcggaaatgg cgccatgtat ttggaaaaag taatttaccc
    11701 agctagacat attgaggtgc aaattttagg agacgctcac ggtaacatca ttcacttggg
    11761 agaacgggat tgctccttgc aacggaataa tcaaaaagtc ttggaagaaa gtccatccat
    11821 tgctattggc aataccctca gacacgaaat ggggcaagca gcggttagag cagctgaagc
    11881 tgtagcctat gaaaatgcgg gaaccattga gtttttgttg gacgaagaca gcgagaaatt
    11941 ctatttcatg gaaatgaata cccgtatcca agttgagcat cctgtcaccg aatttgtgac
    12001 aggagtggat attgtcaaag agcaaatcaa aattgcagca ggtcagcctt tggcgattag
    12061 tcaagaagac atcactatca cgggacatgc tattgaatgt cgtatcaatg ctgaaaatac
    12121 tgcttttaac tttgcaccga gtccaggaaa gattacagac ttgtatatgc caagtggtgg
    12181 tgtaggacta cgagttgatt cagcagttta caatggctat gccattcctc cttattatga
    12241 cagcatgatt gctaaaatca tcgttcatgg aagcaatcga tttgacgctt tgatgaaaat
    12301 gcaacgtgcc ttagttgaat tggaaattga aggcattatc accaatacag acttccagct
    12361 cgatttgatt tcagataagc gagtgattgc aggagattat gatacctctt tcttgatgga
    12421 aacgttcctg ccacattaca ataaagatta ggagaaaata tggctttatt tcgtaaaaaa
    12481 gataaataca tccgtattac tcccaataat tccctcaaag gatctgtcag ccataatgtc
    12541 ccagaagttc cagatgaact ctttgcgaaa tgcccagctt gtaagcacat gatttataag
    12601 aaagatttgg gattggcaaa gatttgccca acctgttctt acaattttag gatttctgcc
    12661 caagagcggc tgaccttaac agttgatgaa ggctctttcc aggaattatt tacaagtatt
    12721 gaaaccaagg atccgttacg ctttccaggt tatcaggaaa aattacaaaa agccaaggaa
    12781 acaactggtc tgcatgaagc tgttttgaca gggaaagcta tggttaaagg acaacaaatt
    12841 gctcttgcta ttatggattc tcactttatt atggctagta tgggaactgt tgttggggaa
    12901 aaaattaccc gattgtttga attggctatc gaagaaaacc tgccggtggt gatttttacg
    12961 gcttcaggag gcgcccgtat gcaagagggg atcatgagcc ttatgcaaat ggccaaggtc
    13021 tctgcagctg tgaaaagaca ttctaatgca ggcctctttt acctcactat tttgacagat
    13081 ccaactactg gtggggtaac agctagtttt gccatggaag gggatattat tttggcagaa
    13141 cctcaatcct tggttggctt tgcaggtcgt cgtgtcattg agaccacagt tcgtgaaaac
    13201 ttgccagatg atttccaaaa agcagaattt ttacaagacc atggttttgt ggatgctatt
    13261 gtgaaacgaa cagagttgcg agacaagatt gcccacttag tagcatttca tggaggtggc
    13321 caatgacaga tgtatcaaga attttaaaag aagcgcgtga tcaagggcgt ttaacaactt
    13381 tggattacgc caaccttatt ttcgatgact ttatggaact gcatggcgat cgccattttt
    13441 cagatgatgg tgctattgta ggtggcttag cttatttggc gggacaacct gttacggtca
    13501 ttggtattca aaaaggtaag aatttacagg ataatttggc aaggaatttt ggccagccca
    13561 atccagaagg ttatcgtaaa gctttgcgcc ttatgaaaca ggcagaaaaa tttggacgac
    13621 cagttgttac gtttatcaat actgcaggag cctatccagg tgtcggtgcg gaagaacgag
    13681 gacagggtga ggccattgct aaaaatttga tggaaatgag tgatctcaag gttcccatta
    13741 tcgccatcat tattggtgaa ggaggctctg gtggtgcatt agccttagcg gttgccgatc
    13801 aggtctggat gctagaaaac accatgtatg cagtccttag tccagaaggc tttgcttcta
    13861 ttttatggaa agatggttca agagcgacag aggctgctga attgatgaaa attaccgcag
    13921 gcgaattgta tcaaatgggc atagtagacc gtattattcc agaacatggt tatttttcaa
    13981 gtgaaatcgt tgacatcatc aaagctaacc tcatcgaaca aataaccagt ttgcaagcta
    14041 agccattaga ccaattatta gatgagcgtt accaacgctt tcgtaaatat taacaacatc
    14101 cttttggagt ctccctacgt gtttatcaag ctaagggagg cctttccaaa agggttttta
    14161 actagtcttg taaataagga aatgggtata ctaataagac ttttgataaa ctagtgtgaa
    14221 aaaaagtgtt gttaaagggg caatgtagtg agtttcaagt gatgtggtgg ctctttaaaa
    14281 tagtaaaccc cagaaatcac tttaatgatc tctggggtga gtttattatt taggtgaaat
    14341 gacggtttcg ccacccatgt aagagcggag tacttcaggg atggtaacag aaccatcttc
    14401 attttggtag ttttctaaaa tggctgctac ggtacgaccg acagcaagtc ctgacccatt
    14461 aagggtgtga agaagtttga ccttaccgtc agcttcatca cggtaacgga tttgagcacg
    14521 tcgtgcttgg aaatcttcag tatttgaaca tgatgaaatt tcacggtagg tgttttgtgc
    14581 aggaatccag acttcaaggt cataggtttt agcagctgag aagcccatgt ctccagtaca
    14641 aagggaaatg acgcgatatg gcaaaccaag tttttgaaga atattttcag cattagcagt
    14701 cattttttct agttcttggt aagattcttc tggtttagca aatttaacca tttcaacttt
    14761 gtggaattgg tgtaggcgga taaggccacg tgtgtcacga cccgcagaac ccgcttcaga
    14821 acgaaaagat gggctcattg cagtaaagta aattggcaat tctttgccgt ctaggatttc
    14881 cccacggtaa taattggtca aaggcacttc agcagttgga atcaggacaa agttggtatc
    14941 tgctaattca aaggtgtcct ctttgaactt tggatattgt cctgtaccaa acatggaatc
    15001 gtggttaacc atgtaaggtg tgatgatttc ttgataacct tctttgatat gttcatccag
    15061 catgaagtta taaagagcac gctccaaacg agcaccaaga tttttataga ataagaaacg
    15121 agcaccagtt acctttgccc cacgttccca gtctaaaata tcaagatctt cgcctagatc
    15181 ccaatgagcc ttaacttcga agtcaaagtc acgaggcgtt ccccaacggc gaatttcgac
    15241 attgtcttct tcgtcagctc caacagggac agaatcgtga ggagtattag gtaaaacagt
    15301 gatgatgtca gtaacttgct ggtcgatagc tactagttga ttgtcaatag ttttgatatc
    15361 tgcagagact ttttgcatat cagcaatttg ttgagtggca tcttcttttt gacgttttgc
    15421 ttgggcgatg gctgcagaag caatattacg ctctgctttt aattcttctg attgcactaa
    15481 tagagcacga cgtttttcat caagttcttt aagatgggtc agggtgtctt cagaaacacc
    15541 gcggtttttt aatttggcag cgacagtgtc aaaatcggta cggatacgtt taagatctag
    15601 catacagaag ctcctttaag atagttatta gtagctgtca gtgaaacaag gctaagtaag
    15661 acagttggat cttgtgattc tctgacgata gaaaaagcac agcttatgaa gatgctggag
    15721 cgctttggcg cggttccatc cagcttcaca atctgtgcac ttaatctcta ttttattgat
    15781 gacacggtag aatttcatat cattttccct atctgttcac agcatccaca gactctctga
    15841 atgaaaaaaa ggtattactt atccgttagg actattataa caaaaaacaa gaaaaagtaa
    15901 actcaaatga gggaatcttt tccactaaat agcaatttag agctactatt ctttgctttc
    15961 ttgaaatgat aaatcagcac tgctcattat ttttttaatg tcgaaaaaat ttgttaataa
    16021 aggtttgcat caaggttggt aatgtaatca ctttctcatt accaatatgt tgacgagtga
    16081 tttttaagat ttttccagag tctccagaag caaataaaaa cttgccttgg tccgtaaaaa
    16141 tttcaaagtg gcggctaact ttttttcttg aaacattagc cccgatgtga ttgatagctg
    16201 tccaaggaat ttgaatataa tcattcacat ttttatcgtt gtaaaattca agggccttgt
    16261 caccgagtaa aatatgacca actttgcctc ctattccaag gtaggagaca gcttttgtct
    16321 tgtattcgac actagtgttt aaagattgag ccatttaagt tcctttcatt gcgtagttgt
    16381 agttattata ccatatttta agtgggattt tcctgacata aagcataaaa aagaagcctg
    16441 agttcatctc agacttccat tggttgatta cataatacct gcaaggtgag caaggatacc
    16501 aaatgcaaac aagccgatga tgattgcgat tggagaaact tttttcttca ataaccacat
    16561 gcaaaggaat gtaaggagta atccagctaa acctgggata agagagtcca attgtccttg
    16621 aagggtttga gcttggactt tatcaaggct cataccgcca atagcgttgg caaggatagt
    16681 tttcaactca gcacctttaa cagcgccatc tgggaagacc acataggcac catctgacaa
    16741 ttgttttcca ggaaggtcga tagtaaagtt gatagatacc caacgttgaa caagcactgc
    16801 aaggataaac attccaagga ttgaagcacc tttagtaatg tcttggagga taccgcctga
    16861 catgtcttta gtaatttcag aaccagcttt gtaaccaaat tcttgagtat accataagaa
    16921 ggccatacgg ataaggttcc atccaaagaa gaacagaagt ggaccaacga tgttacctgt
    16981 tgaagccaaa gaagcaccaa gtgccccaag gataggacga accgtaaacc agaatactgg
    17041 gtcaccgata ccagcaagag gtcccatcat accgatttta accccttgga tagccttgtc
    17101 atcaattggc gtaccatttg cgcgttcttc ttcaagggca agtgttacac caatgattgg
    17161 agctgcaacg tatgggtgag tgttaaagaa ttccatgtgg cgctcaagag cagctgcacg
    17221 gtcttcagga gacgtataca attttttaag agcagggata agtgcataag cccagcccat
    17281 gttttgcata cgttcgtagt tccaagaacc ttgtaagaat tgtgaacgcc accaaacacg
    17341 ttgacggtct gatttagtta atttaatttg ttcagtcatt tgttcttacc cctttctagt
    17401 agtcttctaa gatgtcgccg attggatcag ctgaacctgc tgcattacca ccgttaccac
    17461 cttttttaga aagattaagg tagatgaagg caattgctac accgataaca cccatagcga
    17521 taagggttaa ttggctgata gctgcaagag caaaacctag ggcgaagaat ggccatactt
    17581 cacgagtagc catcatgttg ataaccatag cataacctac agcaacaacc atcgcaccac
    17641 cgatttgcat accttcgttc aaccagtttg gcatagcatt caaaacagat tgtacggctg
    17701 aagtaggaac agcaacaaga agagctgctg ggacagcgat acgcaatcct tgaagaagaa
    17761 gggcaataaa gtggaagcgt tcaatacctg cgaaattacc ttcagtggca gctttgtcac
    17821 cagcgtgtac aagggcagtt gacaaagtac gaacgatcat tgtaaggaaa agaccagcaa
    17881 cagcaagtgg gatagctgtt gagtaagcaa aggtaattcc tttttgagta aagtcgccac
    17941 ttttgaccat gatgatagca gctgcaacag aggcaagggc agcatcaggg gcaacggcag
    18001 caccgatgtt tgcccaacca agagcaagca tttgaagtgt accaccaagg atgacaccag
    18061 cttcaaggtg tccagttaca agtccaatca aagtacaggc aacaagtggt tggtgcattt
    18121 gaaattggtc gaggatacct tcaagaccag cgaagaaggc aatgataacg actaaaattg
    18181 cagaaataat cgaaatatct gacatgttgt aatcctttca aagtatagta aacgaggact
    18241 ttttgctaat aagttagcta gtcatcgttt taaaacctta tagcgtttga aacttattta
    18301 atgttagctt tttggataag ctcaaataag tttttcttag aatcgttagg aactttacgg
    18361 acgtcgaatg taacacctag gtcgcgtaat ttttcaaaag tagcaacgtc ttctttgtcc
    18421 attgataaaa cgttgttaac cattgtttta ccagttgagt gagccattga accaacgttc
    18481 aattcgttga tttcaacacc accttcaacc gcgcgaaggg catcttgagg ggtttggaaa
    18541 agaataagtg cgtgtgtgtt accaaaacga ggatctttag aagcttcaat caatttgcta
    18601 attggaacaa cgttagcttt gacaccacca ggtgcagcct gtttgatcaa ttgtttgcga
    18661 aggtcatctt gagcaacctc atcagaagca acaatgatac ggtcagcctt agaagctgga
    18721 gtccatgctg ttgcaacttg accgtgtaaa agacgagtgt caacacgagc aagattgatc
    18781 tttaatttac catctccgat gacagttcca gcaggaatag ctccttgaag tgcgttaaca
    18841 actttttcag ttgcagcagt ttcttcaact gggttaaggt cttcaggaag ggctttgata
    18901 ccgtccttag attccttaat gatgtttgca gcaacttgtt caacacctgc accagcatcc
    18961 ataagacgtt cagtgtaagc ttgaataagc ataggcaagt tgagaccagt gatgatcgcc
    19021 attttgcggt caggattttc acctgccaca cgactagctt ggttaaatgg agaaccactc
    19081 caaaggtcag ctagcacaag gatttcatca tcagcatcaa attgttggat agcattgttg
    19141 aagtgtccat acaaatcatc tggtccttcg tttggcatga aagtaacaac ttgaactttc
    19201 tcttgttcac caaaaatcat agaaccagat tgatgaatac cttcagcaaa tttaccatgg
    19261 ctggcaataa taataccgat acccattctt gttcttcctc ctttaaaata tttttcgttg
    19321 aaatgaagaa gaataatctc cttccaaaac taaattttgg caattctttt tcacttaaac
    19381 gaaattcaag aacgtttcta ttttaaaacg ttttcactaa aaaatcaagc cctttcggaa
    19441 aaatagtaca aaacatgaaa aaattttcag aatttaggat taaataggca gattattgat
    19501 attaaaggtg ttttcaagct gtttaaaaca aaaatatgct gaaaaatgtt gaaattatta
    19561 aaatgatttt cattttttga tagctgttga cagaagaaaa accaccattt tctgaaaaat
    19621 agtggtttcg tttgctacta caagaaaagt tcttccaatt ttttggcaac accgtcttct
    19681 tcgttggaaa aattaagctg ctgatcagca taaggcagta gcacagggct agcatttttc
    19741 atagcataac cagttccagc aaaagctagc atctcagtgt cattgtgttc atcaccaaag
    19801 gcgattaaat cctttttatc catattgtaa attccaagga gatagttcag agcgtaagct
    19861 ttattgacat tcttagacga aatttcaaga atgttgagag ggcctcccca agaatcaatt
    19921 tcaatttcat ctttgaagaa agcacgcatg tttttagcta aggcatactt gtcttcgtga
    19981 tgcgtttgca tcaataaggc gtttggattt cgagtgattt tggtaatctc taatgccatg
    20041 tcctgagtaa tttcatcaac accaaagagt tgagggtcga tactttctgg attggtcatg
    20101 gtgatgtaga cattttttcg atactcactg gcaataaaat ccatctggaa atcatcctga
    20161 tattttagta accttagtag gtagcctttg tccaaagtga cattatgttc ataggcccat
    20221 ttttgttcag gcatgtgggt caaggcccca ttaaaagtaa tgattggggt ctttaaattg
    20281 agctgcagat agtagtccag tgccatgcgg taaggacggc ctgttgaaat aatgacgtgg
    20341 tggcccttgt cttggacagc tttgatgatt ttttgagtat aggtagagat ggtgttgtca
    20401 tgatgtaata aggttccatc caaatcaatg gcaatcaatt ttttagtcat aattaaatta
    20461 tagcaaattt tgctccaaga taaagagaaa agataagtta agcgtattca ttagtaaaaa
    20521 agataggata actgtttttt taatgaataa atgaagttac agacgttgaa aagtgaacaa
    20581 aaaaatggta acatagtgtt attgttcatt ttaatacgaa caataacaat aataatgagg
    20641 tgtcaaaaag acttatctag tgataagcgc tttttgattt tacctactat ttaattaagg
    20701 agactccatt tcaatggaaa agttttttaa gttaagcgaa aatgggacaa ctgtctcaac
    20761 tgagattatg gctggtttaa cgaccttttt tgccatgtcc tatattttgt ttgttaaccc
    20821 aagtatttta ggtgcagcgg ggatgccctc taatgctgtc tttttggcta cgattatcgc
    20881 agcagccata tcaaccttaa ttatgggact atttgccaat gtgccttatg cgttggcacc
    20941 aggaatggga cttaacgctt ttttcactta tacagttgtt tttgctttag ggttttcatg
    21001 gcaagaagcc ttggcaatgg ttttcatttg tggattattc aatattttta ttaccgtaac
    21061 caagtttcgt aaaagtatca tcaaggcgat tccagttagt ttacagcatg ctattggtgg
    21121 gggaattggt gtctttgtag cttatttagg atttaaaaac gcaaatatca ttactttttc
    21181 tatctctgct gaaaatatag taatggtaaa tggtgttgaa ccggctaaag catcggctaa
    21241 aacatttgca gatggtctat tatttgtaga cgccaatggt ggagttgtac ctacgatttc
    21301 tagttttacg gattctagtg tattacttgc tatttttggt ttacttttaa cgacagctct
    21361 tgtgattcga aattttagag gtgctatttt aattggtatt gtcgcaacaa ctcttgtagg
    21421 tattccttta ggaatagtgg atgtgtccaa cctcaatttt gggatcagcc atattggtga
    21481 agcttggact gaattaggta caactttcct tgcagctttc gatggtttga gttctctttt
    21541 tagcgattca agtcgtttac cgctagtttt catgactatt tttgctttta gtctatcaga
    21601 tacttttgac acaattggta cctttatcgg aactggtcgt cgaacaggta ttttctctca
    21661 agacgatgag aatgctttgg aaaatagtat aggctttagt tcaaaaatgg atcgtgcgct
    21721 ttttgcagat gctatcggta cttctattgg ggctttggtt ggaacttcaa atacgactac
    21781 ctatgttgaa tcagcagcag gaattgctga aggtggacgt actggactaa cagcagtctc
    21841 caccgcagta tgcttcttat tatcaatatt gctattaccg cttgtaggta ttgtcccagc
    21901 tgctgctacg gctccagctt taattgttgt gggtgtcatg atggtgtctt cttttcttga
    21961 tgttaattgg agtaaatttg cagatgctct tccagctttt tttgcagctt tctttatggc
    22021 gctgtgttac tctatttcct atggtattgc cgctgccttt attttctatt gtctagtaaa
    22081 agttgttgag ggaaaaacaa aagatattca ccctattatt tggggagcaa ccttcttgtt
    22141 cattgtaaat ttcatcatat taactatctt ataaccaaat aatttactta ttttgctact
    22201 aactgccgct tctttaattg attttaacct ttaatataac gaagcgtttt tactttacga
    22261 ataccttatt ggagtcatct tctaggtagt gcttggaagt atgttttttt tctgatataa
    22321 tgttgctatg ttttatagtg aaaatgaata tactcttaaa gcttacggag aaaccctagg
    22381 tacttatttg tctattggag atgtgattgt attgtctggc gacttaggtg ccggtaaaac
    22441 aacattggca aaaggtattg ctaaagggat ggggattagt caaatgatca aaagcccaac
    22501 ttatactatt gttagagaat atgaaggacg tctacctctt tatcacttag atatttaccg
    22561 tgttggtgat gatccagact ctattgattt agatgatttt ctttttggta atggtgttac
    22621 cgttattgag tggggagaat tattaggaga gggattatta caagactatt tgcaaataac
    22681 cattactaaa cgagataaag gtcgtcaact agacttgtta gcgcatggtg agcgctctcg
    22741 tcagcttctg gagataatgt cacatgattc agcaaagtct cgttattgaa gaaggcgtac
    22801 ctcaagatgc aaaagctgta ctttccttag tggcggcagc tgccaaagaa acagatttta
    22861 taacaggcgt agagactata ttagaggcta ctcctcatga attatcagac tttttatcaa
    22921 gaagtcaaac ctcttttatt gatttttgcc tgctagctag attagatgaa aaagtagtag
    22981 ggcttttaaa tctttctggg gaagtattat cccaaggcca agttgaagca gatgtattta
    23041 tgttagttgc taagacttac cgtggttatg gtatcggtca attattgtta gagattgctc
    23101 ttgattgggc tgaagaaaac ccttatatcg aaagccttaa gttggacgtt caagtaagaa
    23161 atactaaggc tatttacctg tataaaaagt atggttttcg tattgaaagt atgcgaaaaa
    23221 acgacatcaa atcaaaaaat ggagatgact tagatgttta tcatatgcgt aaatctgtaa
    23281 gtcagtaagt gaatcgatga aaattggaaa aaaaatagtt ttaatgttca cagctattgt
    23341 gttaacaact gtcttggcat taggtgtcta tctaactagt gcttatacct tctcaacagg
    23401 agaattatca aagaccttta aagatttttc gacatcttca aagaaaagtg atgccattaa
    23461 acaaacaaga gctttttcta tcttgttgat gggtgttgat acaggctctt cagagcgtgc
    23521 ctccaagtgg gaaggaaaca gtgattcgat gattttggtt acggttaatc caaagaccaa
    23581 gaaaacaact atgactagtt tagaacgaga taccttaacc acgttatctg gacccaaaaa
    23641 taatgaaatg aatggtgttg aagctaagct taacgctgct tatgcagcag gtggcgctca
    23701 gatggctatt atgaccgtgc aagatctttt gaatatcacc attgataact atgttcaaat
    23761 taatatgcaa ggccttattg atcttgtgaa tgcagttgga gggattacag ttacaaatga
    23821 gtttgatttt cctatctcga ttgctgaaaa cgaacctgaa tatcaagcta ctgttgcgcc
    23881 tggaacacac aaaattaacg gtgaacaagc tttggtttat gctcgtatgc gttatgatga
    23941 tcctgaggga gattatggtc gacaaaagcg tcaacgtgaa gtcattcaaa aggtattgaa
    24001 aaaaatcctt gctcttgata gcattagctc ttatcggaag attttatctg ctgtaagtag
    24061 taatatgcaa acgaatatcg aaatctcttc tcgcactatc cctagtctat taggttatcg
    24121 tgacgcactt agaactatta aaacttatca actaaaagga gaagatgcca ctttatcaga
    24181 tggtggatca taccaaattg ttacctctaa tcatttgtta gaaatccaaa atcgtatccg
    24241 aacagaatta ggacttcata aggttagtca attaaaaaca aatgctactg tttatgaaaa
    24301 tttgtatggg tcaactaagt ctcagacagt aaacaacaac tatgactctt caggccaggc
    24361 tccatcttat tctgatagtc atagctctta cgctaattat tcaagtggag tagatactgg
    24421 ctcatcttat cagagtgcta gtacagacca ggactctact gcttcaagcc atacgccaac
    24481 tacgccgtct tcttcatcag atgctttagc agctgatgag tctagctcat cagggtctgg
    24541 atcattagtt cctcctgcta atatcaaccc tcagacctaa cgttctgagg gtttttatgt
    24601 ttaaaatatc cgattttgaa tataaaaaag acaggtatca aagatgatag ctgtctgtga
    24661 tttcaaataa agtatatgag ggagataata ctatctggaa tggttgttag ttaaaatgac
    24721 ggtttggcaa gtaggtgatt cactagaaat tagctcacta tcttcttgat atttggccat
    24781 acgattttgg atttcagtaa ggtgtgcttg tgtctcttgg ttaaagtagc gccatttatg
    24841 ggccaagtcc tgactaatat tttgaattac ttttccttga ctgtgaatga ggtcaaggtt
    24901 tgctttaatg ttatctaaat caagttgggc agcatctttt gcttgtcgac tgatggataa
    24961 cagctctttt atggtacaac gtttttgata tgctttgtaa gcggtaaaag ataataatcc
    25021 gacggctatc acggttttaa ctttcatgaa ctaacctcct gattgatttg tcgggcgagc
    25081 ttttccaaga caggaaagtt aggctcatga gtggtgtatt gaattgagat accgtctttt
    25141 tcattataac gaggaacgag atgaacatga gcatggaaaa cagtttgtcc cgctaaggct
    25201 tcgttattgt taatgatatt catggcagta gcgccagtag ctaactggat agcacgagca
    25261 attttaggaa tacgagcaaa gagatgacta gctgtctcag cagtcatctc tagtaagtta
    25321 cgaacatgtt gcttaggaat gactagagta tgcccttttg tagtttgtga gatatctaaa
    25381 aaggcgagga cctgctcatc ttcgtagact ttcgaagagg ggatatcccc ttgaatgatg
    25441 ctgcaaaaaa tacaattttc cattttatat caccttctct acttgaattt gttataatat
    25501 attataagta tatcaaaaac tagaaagaaa ttatatgtta aacattaaaa atctaacagg
    25561 aggatatcac aatatccctg ttttaaacga tgtctcattt tctgtagata atggagagtt
    25621 agtcggtttg atcgggctaa atggtgctgg aaagtcaaca actatcaacg aaattattgg
    25681 ctttttgaag ccttatcaag gaagtatttc tattgatggt ttaactctag cagaaaatgc
    25741 agttgcctac cgtcaaaaaa taggttttat cccagaaact cctagtctct atgaagaatt
    25801 gaccttatca gagcatatca acaccgtagc tatggcttat gatatcgact tagaagtggc
    25861 acaaaaacga gcacaacctt tcttggaaat gtttcgttta actgacaaac ttgaatggtt
    25921 tcctgtcaat ttttctaaag ggatgaaaca aaaagtcatg attatttgtg cctttgtcat
    25981 tgatccaagt ctatttattc ttgatgaacc attcttaggg ttagaccctt tagctatttc
    26041 agacttaatc caaacgctag aagtcgaaaa agcaaaagga aaatctattt tgatgagtac
    26101 acatgttttg gactctgctg aaagaatgtg tgatcgcttt gtgattttgc atcatggaca
    26161 agttcgtgcc caaggaactc ttgctgactt gcaagaagct tttggtgacc gttcagcgag
    26221 cttaaatgat atctatcttg cattaacaaa agaggattaa gtatgaaagc attatttctg
    26281 aaacgccgtc aagactttca aaaacaacaa aacaaatacc ttcgttacgt cttaaatgat
    26341 cactttgttt tggtgttgat gtttttatta ggctatgcta tggtgcagta tggtcagcta
    26401 ctaaaccatt ttccaacgaa tcatctacct attcaggttt gtcttgggat attgatccca
    26461 ttattgctca gtatgggatc tatagcaacg tatctagaag aggcagatca gcatttctta
    26521 cttccaaaag aagaagaagt gatttcttat atcaaacaag ctgaacgact ttcttttttg
    26581 ctttggggaa ctttgcagac tgctatattg ctctttttat atcctatttt tagacgtcta
    26641 ggtctgtctt tatttatttt tattatctta gttttgatat tattggcact aaaaagagta
    26701 gtgcttagtc gaaagacaag atattttttg agaggaaatc gtttagattg ggcaaaagct
    26761 gtcgcatttg aaagcaatcg caagcaaagt attttaaagt tttattctct ttttacaaca
    26821 gtcaaaggaa tttctacaaa agtgaaagaa agaacttatc taaatcctct tttaaaatta
    26881 gttaagcaaa ctccttcaaa cctttggcta tccctttatg ctagagcctt tttacgtagt
    26941 tcagattatt tgggcttatt tttacgacta atgctattaa gctcattatc tgtatttttt
    27001 atccataatc tttatttgtc agtaagttta gcactcattt ttaactattt agttgtattc
    27061 caattacttt cactttatta tcactacgat taccattata tgactagtct ttatcctgaa
    27121 aatagtcgga gcaagaagaa aaatgtgtta agttttttac gaggtttaag ctttttaatg
    27181 ttaatcgtca atatgctgtg ttgctcatca gcgccaaaag cactcatttt gattgtaggt
    27241 atggttttca tagcttgtat atatctacct tataagttaa agaagatcat tgactaagta
    27301 gcctaaaaca agtaaaatag gggtaaacga ctataaaaga tagaaaagga gggcgataag
    27361 gtgacaacga cggaacaaga acttaccttg actcccttac gtgggaaaag tggcaaagct
    27421 tataaaggca cttatccaaa tggggaatgt gtctttataa aattaaatac gacccctatt
    27481 ctacctgcct tagcaaaaga acagattgcg ccacagttac tttgggccaa acgcatgggc
    27541 aatggtgata tgatgagtgc ccaagaatgg cttaacggcc gtacattgac caaagaagat
    27601 atgaacagta agcaaatcat tcatattcta ttgcgccttc acaaatctaa aaaattagtc
    27661 aatcaactgc ttcagctcaa ttataagatt gaaaacccat acgatttatt ggttgatttt
    27721 gagcaaaatg cacccttgca aattcagcaa aattcatact tacaagctat cgttaaagaa
    27781 ttaaaacgga gcttaccaga gttcaaatca gaagtagcaa cgattgtgca tggagatatt
    27841 aaacatagca attgggtgat tactactagt ggtatgattt ttttagtaga ttgggattct
    27901 gttcgtctga ctgatcggat gtatgatgtt gcttacctgt tgagccacta tattccacgg
    27961 tctcgttggt cagaatggct gtcttattat ggctataaaa ataatgacaa ggttatgcaa
    28021 aaaattattt ggtatggtca attttctcac ctgacacaaa ttctcaagtg ttttgacaag
    28081 cgtgacatgg agcatgtgaa tcaggagatt tatgccctca gaaaatttag agaaatattt
    28141 agaaagaaat aatgcgagtt agaaaacgaa aaggtgctga ggagcactta gctaataacc
    28201 cacactatgt gatattaaat ccagaagatg ccaagggccg ttggcatgac gtttttggaa
    28261 atgaccgtcc tattcatatc gaagttggtt caggaaaggg cggatttatc acagggatgg
    28321 ccttgaaaaa tcctgatatt aactacattg gcattgatat tcaattatct gtcctaagct
    28381 acgccttgga taaggtatta gcttcagaag tacccaatgt caaacttctg cgagtggatg
    28441 gatcaagttt aaccaactat tttgaagatg gtgaggtgga tatgatgtat ctcaactttt
    28501 cagatccttg gccaaaaacc aagcatgaaa aacgtcgttt gacctacaag gatttcttag
    28561 acacttacaa gcgtatcttg ccagaacacg gtgaaattca ttttaaaacc gataatcggg
    28621 gcttgtttga gtacagttta gctagttttt ctcaatacgg catgaccttg agacagatct
    28681 ggcttgactt acacgctagt aactatgaag gaaatgtcat gacagaatat gaagagaagt
    28741 tctctaataa aggtcaagtc atttaccgag ttgaggcaaa tttctagtag tattttgttt
    28801 caaaatatga taacataaag gagttgatac agtattaact tctttagtaa tggagaggtc
    28861 gcatagtggc ttagtgcgca cgcttggaaa gcgtgtagtc cttaacgggg ctcgggggtt
    28921 cgaatcccct cctctccttg tgagtaaaac aaagtgcata tccttgcatt cctcttaaat
    28981 tatggtataa tgggcttaaa taatacagaa aggtggtggg atatgtctcg ccgcttttgt
    29041 attaagtgga ttcccaagga ccaattatct tagagaagag cataaaaata gaggaggtaa
    29101 tcaaaatagc aaatacatct attatcgata tcgtaaccaa gacagtaaca cctgagatta
    29161 aggcacctta tgaattagta gacgttgaat atgacaaaat gggtagtgac tacatcctta
    29221 gtattttagt cgataaggaa ggtggaatca cagtggaaga tacatctgac ttgacgaata
    29281 ttattagtcc gctgttagat acgattgatc cagatccttt tccaaaccaa tatatgctag
    29341 aagtctctag tccaggtttg gagcgtccgc ttaagacagc tgacagttta aaagcagcag
    29401 ttggctctta tatcaatgtg agtctttatc aagctattga taaggtgaaa gtttttcaag
    29461 gagacttact agcctttgat ggtgaaacgt tgaccattga ttatcttgat aaaacacgtc
    29521 ataagatcgt gaatatacca tatcaagcag ttgctaaggt gagaatggct gtcaaattat
    29581 aacttttatt tcaaagcact aaactttttt aggaacgctt aagcaaattt gaaacaacac
    29641 ctatagagtt tatacaagct aaacaaaagg aattaaggga gcctttaggg ggctattgat
    29701 cgtattaatt taagaaagga tttttgccaa gctttacaag caaggtagat aacattatga
    29761 gcaaagaaat gctagaagcc ttccgtattt tggaagaaga aaaacacatt gataaagcag
    29821 atattattga cgcagtgaca gaatcattaa agtctgccta taaacgtcgc tatggtcaat
    29881 cagaatcatg tgttattgaa tttaatgaaa aaacagctga ttttcaagta ttcacggttc
    29941 gtgaggttgt tgaagaagtt tttgacagcc ggcttgagat tagtttgaaa gatgctcttg
    30001 ctatcagttc tgcctatgaa ttgggtgata agattcgctt tgaggaatct gtcaatgaat
    30061 ttggacgtgt cgcagctcaa tcagctaagc agactattat ggaaaagatg cgccgccaaa
    30121 tgcgtgaagt gatgttcaat gaatacaaag agcatgaagg tgaaattatg acaggaacag
    30181 ttgaacggtt tgatcaacgt tttatttatg tcaatcttgg ttccttagaa gcacaattgt
    30241 ctcaccaaga ccagattcca ggtgaaactt tcaaatcaca tgaccgtatt gacgtttatg
    30301 tctacaaagt tgaaaataat cctaaaggag ttaacgtctt tgttagtcgt agccatccag
    30361 aattcatcaa acgtattatg gagcaagaaa ttcctgaagt ctttgatggt actgttgaaa
    30421 tcatgagtgt ctctcgtgaa gcaggtgacc gtaccaaggt tgctgtgcgc agtcataatc
    30481 ctaacgttga tgccatcgga acaattgttg gacgtggtgg aagtaatatt aaaaaggtca
    30541 tcagcaagtt ccatccaaaa cgtgtagatg ctaagacggg gcttgaaatt ccagttgaag
    30601 aaaatattga cgtgattcaa tgggtagatg atccagctga gttcatctac aatgccattg
    30661 ctccggctga agtagacatg gtactttttg acgatgaaga tcttaaacgt gctacggtag
    30721 tagttcctga tagcaaatta tcattagcta tcgggcgtcg cggccaaaac gttcgtttag
    30781 cggcacattt gactggctac cgtattgata tcaaatcagc tagcgaatat gatcgtcttg
    30841 aggcggaaaa agaagcagca acagttgtgg aagagccagt tgttgacgat gttatgactg
    30901 ccgaggaagt gtaagaaaag aggtgtttga tgtctaaagt caaaaaaata cctttacgaa
    30961 aatcacttgt ttctggtgaa attattgcta aacgagatct tcttaggatt gtaaaaacta
    31021 aggatggtca agttttcatt gatccaactg gtaaacaaaa tggtcgtggt gcttatatca
    31081 aattagacaa tcaagaggcc ttaatggcca agaaaaaaca agttttcaac aggagtttct
    31141 caatggacat tccagaaagt ttttacgatg acttgattgc ttatgttgat cacaaaatca
    31201 aaagaagaga gttaggtctt gactaattta gaaagattat ctagtcttat tggtctagct
    31261 caacgggctg gaaaagtgat ttcaggtgaa gaactagtag taaaagctat tcaacaccag
    31321 caggtaatat tagtattttt agccaatgat gctggtccta atgtgacaaa aaaagtgaca
    31381 gataaaagta attattataa tgtagaagtc tccacagtgt tgaatgcact ggaattaagt
    31441 gctgcactag gtaaaccaag gaaggttgct gccgttgcag atgctggatt ttcaaagaaa
    31501 atgaggactc ttatggaata gatgaatagg aggacacaaa ttgtcaaaga aaagattaca
    31561 tgaaattgcc aaggaaattg gtaaaagtag taaagaggtg gttgaacacg ctaaatactt
    31621 aggattagat gtgaaaagcc atgcatcaag tgttgaagaa gcggatgcga aaaagattat
    31681 ttcaagtttt tcaaaggctt ctaaacctga tgtgacagct tctcaaacag tcaagcccaa
    31741 agaagttgct caaccctctg taacagtggt caaagaaaca ggttctgagc atgtagagaa
    31801 aacacaggtc tctaagccaa agagtcgtaa ttttaaagct gaaagagagg ctcgtgcaaa
    31861 ggaacaggct gctcgaaaac aagcaaatgg ctcttctcat cgttctcaag aacgtcgagg
    31921 aggttatcgc cagcccaata atcatcaaac taacgaacaa ggagataaga ggattaccca
    31981 tcgttctcaa ggtgatacaa acgataagcg tattgagcga aaagcatcga atgtatcacc
    32041 tagacatgat aatcatcagc tagttggtga tcgtaaccgt tcctttgcta aggagaatca
    32101 caagaatggt cgattcacaa accagaaaaa gcaaggacga caagagcctc aatcgaaaag
    32161 tcctaaaatt gatttcaaag ctcgtgctgc tgccttaaaa gctgaacaaa atgctgaata
    32221 ctcacgccaa agtgaaacac gttttcgtgc acagcaagaa gctaagcgtt tagctgagtt
    32281 ggcaagacaa gaagctaaag aagcggcttt aaaagcacaa gctgaggaga tgagccatcg
    32341 tgaagcagca ctgaaatcta ttgaagaagc agaaactaaa ttaaagtcat caaatattag
    32401 tgctaaatct actgctgata atcgccgtaa aaaacaagca cgtcctgaaa agaatcgtga
    32461 gttaactcat cattcacaag aaggacaaaa gaaaaataag aagagttgga atagtcaaaa
    32521 ccaagtgaga aatcaaaaaa atagtaactg gaataaaaat aaaaagacca aaaaaggcaa
    32581 aaatgttaag aatactaaca ccgctccaaa accagtaacc gagcgtaaat tccatgaatt
    32641 gccaaaagaa tttgaatata ctgaaggcat gacagttgcc gaaatcgcaa aacgtatcaa
    32701 acgcgaaccc gctgaaattg ttaaaaagct gtttatgatg ggtgttatgg caactcaaaa
    32761 ccagtcttta gatggggata ctatcgaatt attgatggta gattatggta ttgaggctaa
    32821 ggctaaggta gaagtagatg atgctgatat cgagcgcttc tttgaggatg aaaattacct
    32881 caatcctgaa aatattgtag aacgcgcacc tgttgtaact atcatgggac acgtagacca
    32941 cggtaagaca actttgcttg atactttgcg taactcacgt gtggcaacag gagaagctgg
    33001 aggaattact cagcatattg gtgcttacca gattgaagaa gctggcaaga aaattacctt
    33061 cttggatact cccggacacg cggcctttac aagcatgcgc gcacgtggag cttccgttac
    33121 cgatatcacc attttgattg ttgcagctga tgatggtgtt atgccacaaa cgattgaagc
    33181 gatcaatcac tcaaaagctg ctggtgttcc aattattgtt gctatcaaca aaattgataa
    33241 accaggtgct aatccagaac gtgttatcgc agaattggca gaatatggta tcatctcaac
    33301 tgcttggggc ggagagtgcg aatttgtaga gatatctgct aaatttaata aaaatattga
    33361 tgaactatta gaaacagtcc ttttggttgc tgaagtagaa gaattgaaag ctgatccgac
    33421 ggttcgtgct attggtactg tgattgaagc acgcttagat aaaggtaaag gtgctatcgc
    33481 tacgcttctt gtccaacaag gaactcttca tgttcaagac ccaatcgttg ttggtaatac
    33541 ctttggtcgt gtccgtgcca tggtaaatga ccttgggcgt cgtgttaaat ctgcggagcc
    33601 atcaacacca gtttccatta ctggtttgaa cgaaacacca atggctggtg atcactttgc
    33661 tgtttacgct gatgaaaaag ctgcgcgtgc agctggtgag gagcgatcaa aacgtgccct
    33721 cctcaaacaa cgtcaaaata cacagcgtgt tagccttgat aatctctttg atacccttaa
    33781 agcaggagaa atcaagactg ttaatgtcat catcaaagca gacgttcaag gttctgttga
    33841 agcccttgca gcatctcttg taaagattga agtggaaggt gtccgtgtca atgtcgttca
    33901 ctctgctgta ggtgctatca atgagtctga tgtgaccctt gctgaagcat caaatgctgt
    33961 tattatcggt ttcaacgttc gccctacccc acaagctcgt caacaagcag atacggatga
    34021 tgtggaaatt cgtcttcata gcattatcta taaagtcatt gaagaagtag aagaagccat
    34081 gaaagggaaa cttgaccctg tttaccaaga aaaaattctt ggtgaagcca ttattcgtga
    34141 aaccttcaaa gtttctaaag tgggaacaat tggtggcttt atggtaatca acggtaaggt
    34201 gacacgtgat tcgagtgttc gtgttatccg tgatagtgtc gttatttttg atggtaaact
    34261 ggccagcttg aaacattaca aagatgacgt taaagaagtc ggtaatgctc aagaaggtgg
    34321 gttgatgatt gaaaacttta acgatcttaa agttgacgat actattgaag cctatattat
    34381 ggaagaaatt gtgagaaaat aggggctaag aaggtctgag atgtgacgtt tttcctctgt
    34441 cggaaataat gacttgttct accagatgag ctacttgggg cattccttcc agtctcgtca
    34501 tggaattctc agattccctt tagttgtcgt tatcaaaaca ggtagcaatg tcataagctg
    34561 ggctttcagc ctagcttttg ttttaaaaat gtggaaagga aatggctatg gctaatcatc
    34621 gtattgaccg cgttgggatg gaaatcaagc gcgaagtaaa tgatattctt cagaaaaaag
    34681 tgcgtgatcc acgcgttcag ggagtaacga ttacagaagt gcaaatgcag ggtgacttgt
    34741 cacttgctaa agtttattac actattatga gtgacttggc atctgataat caaaaagcac
    34801 aaacagggct tgaaaaagcg actggaacga ttaaacgtga acttggcaaa caattgacca
    34861 tgtacaaaat tccagatttg gtgtttgaaa aagacaattc catcgcctat ggtaataaaa
    34921 tagatcaact gttacgtgag cttgataaaa aagattaaat taaaaacctt taaggtatct
    34981 tattagagtc accttaaggg ttttttgttt ttagtaagct ttttgaatcg tatcaatgat
    35041 ttgatattgt gcagtcttgg cttcttcaat tggatagatt gggtaaacat gattcatttt
    35101 atcagcgacg ataagttcat gttgatgacc tttggcagtt aatatatcat caaatttctt
    35161 aacatctggg tagaaaattt catgtgtacc aacaaacata gtaataggtg ctagccctga
    35221 tacatctcca ttaattggac taacatatgg atgtttggtg ttgtttgggt cacggcgtgc
    35281 ccaaatttcc ccaactttac gtaggcccca agctgaaaga atgggatcat tactttcata
    35341 gctctcaata tctggatttg acatggttac atctaaccat ggtgataaga gaacaatgtc
    35401 ttttggttgt tttaaacttt cattttttag ggctaatgct aaaccaagcg ctagtccacc
    35461 acctgctgaa tcacccataa tagtgatatt ttgtgcagtt gtttctttaa gagtatctct
    35521 gtaaaggtca accaattttg gcatagcatc actgtaagag taagtaggtg cttttggata
    35581 gattggtaag ataatcttag catcaagact agttgataag gtttttagca tgtcgatatg
    35641 ataagtagtt gggttgttta ggtaagagcc cccatgtaaa tagaaaatga ccttttgatt
    35701 ggctttattg ctggggttct cattccaagt atgaacttgc atattatcaa cagttttagt
    35761 gactaatgtg ccatttacct gttcttcttt aagatcaaag cctttatttg aagcttctcc
    35821 agcagagttg acatattgat caaacttagt ttcgtcttgc tctgaataat agcctttgtc
    35881 tccttttaac caaaagtatt tctctataaa ccagcttttc caagatctag aactggcatc
    35941 aacttgagtg ctagctccta ttgctagagt aagtcctagt aaaataagag tgcttttaag
    36001 tgtctttttc aatgagttct ccttattatt atcatttttt tatcatataa tcatatcata
    36061 aatg
//
LOCUS       DATXIG010000015        32410 bp    DNA     linear   BCT 28-DEC-2023
DEFINITION  TPA_asm: Streptococcus pyogenes strain MGAS11113
            SAMN08555799-rid19682583.denovo.015, whole genome shotgun sequence.
ACCESSION   DATXIG010000015 DATXIG010000000
VERSION     DATXIG010000015.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN08555799
            Sequence Read Archive: SRR6740495
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pyogenes
  ORGANISM  Streptococcus pyogenes
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 32410)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 32410)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-OCT-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-OCT-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001918058.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001918058.2
            Genome Coverage       :: 368x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/12/2023 11:23:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 1,806
            CDSs (total)                      :: 1,769
            Genes (coding)                    :: 1,722
            CDSs (with protein)               :: 1,722
            Genes (RNA)                       :: 37
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1, 1 (5S, 23S)
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 47
            CDSs (without protein)            :: 47
            Pseudo Genes (ambiguous residues) :: 0 of 47
            Pseudo Genes (frameshifted)       :: 30 of 47
            Pseudo Genes (incomplete)         :: 20 of 47
            Pseudo Genes (internal stop)      :: 9 of 47
            Pseudo Genes (multiple problems)  :: 11 of 47
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..32410
                     /organism="Streptococcus pyogenes"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN08555799-rid19682583.denovo.015"
                     /strain="MGAS11113"
                     /serotype="Emm28"
                     /isolation_source="normally sterile site"
                     /host="Homo sapiens"
                     /db_xref="taxon:1314"
                     /country="Finland"
                     /collection_date="2002"
                     /collected_by="National Public Health Laboratory"
     gene            284..1036
                     /locus_tag="VC122_000737"
     CDS             284..1036
                     /locus_tag="VC122_000737"
                     /EC_number="2.5.1.54"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002983746.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-deoxy-7-phosphoheptulonate synthase"
                     /protein_id="HEP5640680.1"
                     /translation="MIDIMSDFQNKTCSKNNFIVGPCSIESYDHIRLAASSAKKLGYN
                     YFRGGAYKPRTSAASFQGLGLQGIRYLHEVCQEFGLLSVSEIMSERQLEEAYDYLDVI
                     QVGARNMQNFEFLKTLSHIDKPILFKRGLMATIEEYLGALSYLQDTGKSNIILCERGV
                     RGYDVETRNMLDIMAVPIIQQKTDLPIIVDVSHSTGRRDLLLPAAKIAKAVGANGIMM
                     EVHPDPDHALSDAAQQIDYKQLEQLGQELWQD"
     gene            1092..2165
                     /locus_tag="VC122_000738"
     CDS             1092..2165
                     /locus_tag="VC122_000738"
                     /EC_number="4.2.3.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011018028.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-dehydroquinate synthase"
                     /protein_id="HEP5640681.1"
                     /translation="MPQTLHVHSRVKDYDILFTDHVLKTLADCLGERKQRKLLFITDQ
                     TVYHLYQTLFEEFAQQYNAFVHVCPPGGQSKSLERVSAIYDQLIAENFSKKDMIVTIG
                     GGVVGDLGGFVAATYYRGIPYIQIPTTLLSQVDSSIGGKVGVHFKGLTNMIGSIYPPE
                     AIIISTTFLETLPQREFSCGISEMLKIGFIHDRPLFQQLRDFQKETDKQGLERLIYQS
                     ISNKKRIVEQDEFENGLRMSLNFGHTLGHAIESLCHHDFYHHGEAIAIGMVVDAKLAV
                     SKGLLPKEDLDSLLQVFERYQLPTTLERADVSATSLFDVFKTDKKNSEQHIIFILPTE
                     TGFTTLAINKDDHQFVEKLDSLL"
     gene            complement(2600..2905)
                     /locus_tag="VC122_000739"
     CDS             complement(2600..2905)
                     /locus_tag="VC122_000739"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002989014.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cupin domain-containing protein"
                     /protein_id="HEP5640682.1"
                     /translation="MIENLSQLVQYHDHQVASRSLSRLLGLSQSIVLYAMAQGESISQ
                     ETSPRDKVILVLEGQLIFDLEDQKQVLTQESLIAIPAQKAHHLEAKTDCKLLQVLLD"
     gene            complement(2907..3245)
                     /locus_tag="VC122_000740"
     CDS             complement(2907..3245)
                     /locus_tag="VC122_000740"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002265614.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cupin domain-containing protein"
                     /protein_id="HEP5640683.1"
                     /translation="MSYINNIEHAKVLDLTQEVMIEQDQMLSRTLVQRQDLGITVFSL
                     DKGQEIGRHSSPGDAMVTILSGLAEITIDQETYRVAEGQTIVMPAAIPHALYAVEAFQ
                     MLLVVVKPEA"
     gene            complement(3298..4053)
                     /locus_tag="VC122_000741"
     CDS             complement(3298..4053)
                     /locus_tag="VC122_000741"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010922518.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase domain-containing protein"
                     /protein_id="HEP5640684.1"
                     /translation="MVTKEVGHDFLARLGKKRLRPGGVEATNWLIEQGQFSQDCHVLE
                     VACNRCTTAIELAKTFGCQIDAVDLNPKVVKEAQERVTVEGLTRHITVSQANALSLPF
                     PDNHFDIVINEAMLTMLNAKAKEKALNEYWRVLKPGGRLLTHDVAYEDPKTRQVLDEL
                     RQTIHVNVAPLSLTGWQALYQEHGFSQVTYHHGKMSLMTPRGMIKDEGLMNTLNIMRR
                     GLKKENRQQFLKMRRFFTRTGKNLCYIAVKSVK"
     gene            complement(4305..5183)
                     /locus_tag="VC122_000742"
     CDS             complement(4305..5183)
                     /locus_tag="VC122_000742"
                     /EC_number="1.1.1.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020905350.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="shikimate dehydrogenase"
                     /protein_id="HEP5640685.1"
                     /translation="MSERLSGHTLLVSLLATPIRHSLSPKMHNEAYAKLGLDYAYLAF
                     EVGTEQLADAVQGIRALGIRGSNVSMPNKEAILPLLDDLSPAAELVGAVNTVVNKDGK
                     GHLVGHITDGIGALRALADEGVSVKNKIITLAGVGGAGKAIAVQLAFDGAKEIRLFNR
                     QATRLSSVQKLVTKLNQLTRTKVTLQDLEDQTAFKEAIRESHLFIDATSVGMKPLENL
                     SLITDPELIRPDLVVFDIVYSPAETKLLAFARQHGAQKVINGLGMVLYQGAEAFKLIT
                     GQDMPVDAIKPLLGDE"
     gene            complement(5321..8827)
                     /locus_tag="VC122_000743"
     CDS             complement(5321..8827)
                     /locus_tag="VC122_000743"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011528838.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 2 protein"
                     /protein_id="HEP5640686.1"
                     /translation="MVYRQKSSEIVTRTITKPSRATSNVKQEIDMTPDSKEQTVTGYQ
                     YHYIDQEGRKQPFNQGWRFLMADVACAQDPSFDDSNWQVIHLPHDFSLTQPYTRNGEA
                     ESAYKLGGVGWYRHYLVLDEVLAGCHVAITFEGSYMETEIYVNGQFIGKHLNGYQEFT
                     YDISDVVTFGAENLLAVRVENKVPSSRWYSGSGLYREVSLSVLPQLHFVADQVAMTLA
                     DTAVQEKGQQKVDLRFALNQSIQTCHYQLSLCLWEQSHCSKDKKLLYQETEVPLADLA
                     FQRQYGLTLSLEELQLWSPDNPHLYDLELTLYYQGQVIDCFCLETGFRQLTFMANQGL
                     FVNGRAVKLKGVCLHHDQGGLGACAYEDALARQLVLLKDMGANTIRSTHNPSSPKLRQ
                     LANRLGFFVIEEAFDTWTYAKNGNVNDFSNYFHQTIGTENASYLQRVRSPETSWAQYS
                     IEAMVWSAKNDPSVLMWSIGNELMEGFSADVSHYPELTRQMCQWIAAIDTSRPITFGD
                     NKLKEADFCWHEEVSQMATLLSQLDHPQGLIGLNYANGKDYDKLHEEHSDWLLYGSET
                     ASAITSRAYYKETKKVLDSGYHLTSYDHAKVDWGAFASQAWYDTITRDFVAGECVWTG
                     FDYLGEPTPWNKIDSGVVGLWPSPKNAYFGILDTAGFPKDSYYFYQSQWAQGQTTLHL
                     LPVWQKDQLCFDEQGLVEVVVYSNAASVQLMFEDEQGNVTDYGRKAFHTYSTPTGHTY
                     QLYQGADAAKNPHENLYLTWRVPYQKGLLRAVAYDISGKSIPKTSGRSQVRTYGSVAK
                     LSWKAFEAPIDAPWELLYLDLSLLDSRGELVSHAQDWLKVQVEGPAHLLALDNGNPTD
                     HTPYQEPLRQAYGGKLLAILALTGEAGHIKVTAKVSQQLSSVFQTQVACRAPKEHLVT
                     LPLEKYQFKKSRLTSQQEIGQSCLKTTQFLSVLENKDTQQKAGKVSTPHILITSYQHY
                     LSLPLQVCSQAFDFKKGDDIFLPQWLQVKDAQGSFVKKAFPMTWYLKEKIFAKPILVT
                     GYVSCFGQSIPVEARLNLAQASKRRHQDLSLVAKQSLATSQKRLTYCYQYDTVQAIGA
                     ISLQNHTDTSLSVSVAISYGSQSSIKQETLMLADSHLSQLEEVIFFDDILTVLYLVVT
                     LEVIEGPDLTEDSIQIGLWDLA"
     gene            complement(8757..10241)
                     /locus_tag="VC122_000744"
     CDS             complement(8757..10241)
                     /locus_tag="VC122_000744"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012560842.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HEP5640687.1"
                     /translation="MYKVLLVDDEYMILQGLTMIIDWQALGFDVVSTARSGQEALAYL
                     TQYPVDVMISDVTMPGMTGLDLIEAAKTNHPQLQTLILSGYQEFSYVQKAMELETKGY
                     LLKPVDKAELQAKMKQFKDWLDAQQAESIRQETYHDSLLTLWLTDELSEKEFHQLSQD
                     LPEHALTGFTVLYVECSEWIEEMEAYFKQEEQPCYLKKEEDKKIYLAILLGRAQQAKA
                     FTSNLQKQFSENIQQIILGETVDDWENVYESYNQVRQSLFYNSQMSQPTDKGNSKLEM
                     PESRLQFFAFNKALMIGDEPTILAKLEAIFHEMKALNFSPEDVKHVSFLLFSDIYRQF
                     PILDKMTYLSMVKTIHNSQSIDRILRELKKVLDVTNQNNSPEKRYSDLVSETIDCIRK
                     EYHQELTLKAIADRLHVNGVYLGQCFKNETERSFTQYLNHVRIQKAQQLLLYTNQSIN
                     EIAYETGYNTNHYFIKMFKKLNGLSPKEFRDRYKDNYQAIKGDQ"
     gene            complement(10241..11965)
                     /locus_tag="VC122_000745"
     CDS             complement(10241..11965)
                     /locus_tag="VC122_000745"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015057952.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sensor histidine kinase"
                     /protein_id="HEP5640688.1"
                     /translation="MSVKTLGRYVTNHFKQGFLINRLMKLYSLLIVSFFTLAAIGLSS
                     YSISSTYKRVDAEAQMRLEETLGRLQLQNDITLRVLDQLVGRSEDYHNLYQYMTLTPN
                     QYFSNVFEDWEKGKDTVLFSEEVRRLFDLYPDVTSMTLLLEDSSAYLYADRVIKTGRL
                     LYSKPEKVTGNVLVRSIRNPESGDVTGRLYLTFDKPPTLVETQQDHYLATFAFDYYGR
                     KLFHQGGRRFPHLEAEVKKAIKADRAADLSNLSKAYRMQYNRSGDLLAYVAVRKSYLL
                     AEAVRTVFVYGLVSLLLAWLLLQLLFRVFRNYIQQVSEITDTVEMVAAGDLSLTIDNS
                     HMELELYHISEAINQMLASIKAYIDEVYVLEVEQRDAQMRALQSQINPHFLYNTLEYI
                     RMYALSCQQEELADVIYAFASLLRNNISQDKMTTLKEELAFCEKYIYLYQMRYPDSFA
                     YHVKIDESIADLAIPKFVIQPLVENYFVHGIDYSRHDNALSIKALDETDHLLIQVLDN
                     GRGISQERLADMEKRLQEHQTTGNSSIGLQNVYLRLFHHFRDRVSWSMAKEPNGGFII
                     QIRIRKDV"
     gene            complement(11955..12560)
                     /locus_tag="VC122_000746"
     CDS             complement(11955..12560)
                     /locus_tag="VC122_000746"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002995651.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YesL family protein"
                     /protein_id="HEP5640689.1"
                     /translation="MNGIEKTCFLIWKMIQLTLIFHLLSLAGLIVFGVGPAWQTIVTL
                     FLKTPQEENHYSLKRAVQLWKSYFKEANLRFGLFALTFLFLTFNLHWAVQFPSLFWFT
                     VSFLIVIAMVWLTITYLYMVFYAVSYEINLWNNMKLAFISVFLSFKSFLLMLSVLLGI
                     TLMTWQYKGLYLFLTFGALVFCLDFVTKANRRLVDGVIDEC"
     gene            complement(12866..14311)
                     /locus_tag="VC122_000747"
     CDS             complement(12866..14311)
                     /locus_tag="VC122_000747"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003044577.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="extracellular solute-binding protein"
                     /protein_id="HEP5640690.1"
                     /translation="MKKWQKIVCVTGTVLAASSLAACESKSASKDSDVKLLMYQVGDK
                     PDNFDELMTIANKRIKEKTGATVDLQYIGWGDWDDKMSTIIASGENYDIAFANNYVVN
                     AQKGAFADLTTLMPKYAKKTYKNLDPAYIKGNTIDGKLYAFPVDANVYAQQMLSFNKE
                     LVDKYGLDISNIKSYADAENVLKQFHEKEPNTAAFAIGQVFSMSGDYDYPLTKTQPFA
                     VKIDEGKPTIINQYEDESFKNNLRLMHKWYKEGLIPTDAATNTEGYPLEGNTWFMREE
                     TQGPMDYGDTILTNAAGKDIVSRPLTKPLKTTSQAQMANFVVSSVSKNKEKAVEVLSL
                     LNSDPELLNGLVYGVEGKAWEKIGDKKIKLLDGYQPKMHMGAWNTGNNKILYTQESVT
                     DDMIAKRDQSIKDAKESPILGFTVDTKVIKTELSNISNVMNRYKASINTGTVDPDEAL
                     PKLLADLKGAGWDKVQKEVQKQLDDFVAKDK"
     gene            complement(14392..15318)
                     /locus_tag="VC122_000748"
     CDS             complement(14392..15318)
                     /locus_tag="VC122_000748"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003044580.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HEP5640691.1"
                     /translation="MEKKKKVEKVNVRTFDRKTNAIFNILIGLFAISCIIPFIFVIII
                     SFTDESYLINHGYSFFPDVWSTKAYQYIFQGAMSHRIMRSFGISVFITVVGTFINTTM
                     TSTYAYAISRPYFPYRRFFTVYALITMLFAPGMVANYLVVSNLLHLKDTVWALILPMA
                     LGPFGILVMRTFFKKTVPDSIIESARMDGASEWMIFMKIVLPLAVPGIATISLFSALT
                     YWNDWFNALLYVQSENLYPMQYLLMKIQSNLQALAQNAGMSAQMADSLASLPKESVRM
                     AIVVIATLPIALTYPFFQKYFVGGLTIGGVKE"
     gene            complement(15328..16278)
                     /locus_tag="VC122_000749"
     CDS             complement(15328..16278)
                     /locus_tag="VC122_000749"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004219264.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HEP5640692.1"
                     /translation="MTRQTNKKTSFWKNVIKYRALLLMVLPGFIWFIFFFYIPVLANV
                     VAFKDFHYSAGGFMESLKESPWVGLANFKYLFASKDAWLITRNTIAYNVIFLLFNVFF
                     AIVFAIIMSELRNKRTVKVYHTMSLLPYFLSWVVIEYFVSAFLNTDKGFINQLLVGSG
                     ADPIKWYSNPTWWPLILLFMSGWKGLGYNSIIYYASVKGISDTYYEAAMVDGASKWQQ
                     IRNITIPQLLPMMSILLIINIGNIFKSDFGLFYVIPKNSGPLYDVTSVLDTYVYNALT
                     ATGDIGMASAASLYQSVVGTSILLVTNAIVRRMDPDAALF"
     gene            16474..17352
                     /locus_tag="VC122_000750"
     CDS             16474..17352
                     /locus_tag="VC122_000750"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011888708.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ROK family protein"
                     /protein_id="HEP5640693.1"
                     /translation="MSLLCIDIGGTSLKFALCHNGQLSQQSSFPTPSSLEKFYQLLDQ
                     EVARYSAYHFSGIAISSPGAINKEKGVIEGASAIPYIHHFKIQEALEERLHYPISIEN
                     DANCAALAEATLGAGKGASSLAMLVLGTGVGGSLVIDGKIYHGAHLFGGEFGFMIMND
                     RYQTFSQLGTVVNMAKRYSAIVNNGKDYTGKAVLALAEQGDHLALKERQVFLQSLAIG
                     IFNIQHAFDPQLILIGGGVSQADFLLSAIEAELDKLYQAVEISDLRPQLAICHFKNEA
                     NLLGAAIDFYQEHKEA"
     gene            complement(17963..19405)
                     /locus_tag="VC122_000751"
     CDS             complement(17963..19405)
                     /locus_tag="VC122_000751"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002983711.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 1 protein"
                     /protein_id="HEP5640694.1"
                     /translation="MLAFPKEFWWGGATSGPQSEGRFAKQHRNLFDYWYEEEPDLFYD
                     YVGPDTASDAYHQIESDLALLASLGHNSYRTSIQWTRLIDDFEQVTINPDGLAYYNRV
                     IDACLANGIRPVINLHHFDLPIALYQAYGGWESKHVVDLFVAFSKVCFEQFGDRVKDW
                     FVHNEPMVVVEGSYLMQFHYPAIVDGKKAVQVAYNLALATAKVIQAYRQGPAELSDGR
                     IGTILNLTPAYPASQSEADMAAAHFAELWNNDLFMEAAVHGKFPEELVAVLKKDGVLW
                     QSTPEELALIAENRVDYLGLNFYHPKRVKAPDVIPVISPSWSPEWYYDAYLMPGRRMN
                     VDKGWEIYPEAVYDIAIKMRDHYDNIPWFLSENGVGISGEDRYRDETGQIQDDYRIRF
                     LKEHLTYLHKGIEAGSNCFGYHVWTPIDGWSWLNAYKNRYGLVENNIHTQVRRPKASA
                     YWFKKVATHNRLISLEVMEEFGGSASHLSD"
     gene            complement(19430..21124)
                     /locus_tag="VC122_000752"
     CDS             complement(19430..21124)
                     /locus_tag="VC122_000752"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010922528.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-N-acetylglucosaminidase"
                     /protein_id="HEP5640695.1"
                     /translation="MTIYHALKDYQEVMTRGDYLVFDMPLTCRFIGRFFRFENQKALL
                     AELATSKYFQWIEEGQAELTVKHFFNRQLDKDAFTLKVSEDKEIIIESQNLRGFRYAQ
                     EALLKVMTFKGDKLYLPIVSVKHSPSFAMRGIIEGFYGTPWTREERLDCLRFIGRKRM
                     NTYMYAPKDDDYQRKLWRDLYPEDWVTYFKELLAVAKEEGIDFWYMISPGLDFDYTKE
                     ADYQLLYQKLQQLLALGVCHFGLLLDDIDYQIVDAVERRFKKTAYAQAHLATEVHHFL
                     NQQHAAPELVICPTEYDNHHDSIYLQELSERIPKEVAFFWTGPSTLASQISQADIETM
                     AAVYQRPIIIWDNIPVNDYQKDPERLFLTPFANRSPFLCQSDYQVKGIVSNPMISWEL
                     SKLTLTDMSHYLWDANRYQPSNSWLETLTDYTGDAELALALQAFAWHNGNRHLHRDLP
                     FEVEEALLAKDVSTLSAWVDELVERVNTLRKLDKPAFQQAIVPWFERVAKDQDFWQAI
                     LEQGPHLETLYADLQEDKHRIGSDIPSRYYRIYYQQQDKLTANQGQVTQARPEDYA"
     gene            complement(21175..22215)
                     /locus_tag="VC122_000753"
     CDS             complement(21175..22215)
                     /locus_tag="VC122_000753"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011054789.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HEP5640696.1"
                     /translation="MTKLLYKKIYHFIKEQIASGRLQIGDRLPTEKELSEQFSVSRIT
                     SKRALVELEQEGLITRSRGKGSFVAEKQVTSPEANKDLLLILPFTSDYELGDYAKGIM
                     TSIAETGYRLMVQLASTVRLDTLSDYAGIIYYPEDVNHSIDFLFYCDHHHIPLVLLDK
                     SLDLFQFPSVVADNKGGACQLTQHLIEQGCDQISFVATGSFGEVSSVRDRYLGYLAAM
                     AESSLKSHYFPKTETETTDAYLDRLVKTLSETTATKRGLVVENDWLAIQLIQKITQVG
                     LYIPDQVAIVGFDNSQASRLLSPKLSTAAQDFYQMGQEAAKVLLQIIHEPDQAVVSCQ
                     LPVQVFIRESSH"
     gene            22348..23634
                     /locus_tag="VC122_000754"
     CDS             22348..23634
                     /locus_tag="VC122_000754"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011285655.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 125 protein"
                     /protein_id="HEP5640697.1"
                     /translation="MTRPIPTSVSQFMAKVESLCGDQHPDWALNFKTSFTNTLETTLK
                     TYEDGTSFLLTGDIPAMWLRDSTAQMKPYLFLAKEDEEIRKIIAGLVKRQFRYICIDP
                     YANAFNEEANGKGHQTDRTQMNPWIWERKYEIDSLCYPIQLAYLLYRETGSTDQFNDD
                     FHRGVELILDLWTVEQDHAQSPYLFERDTWRKEDTLTHAGKGSPVAPTGMTWSGFRPS
                     DDACQYGYLIPSNMFAVVVLSYLEDLYNNLFHNEPVATRAKQLKEAIQSGIADHALVQ
                     NSKGETIYAYEVDGLGQFSIMDDANIPSLLAAPYLGFCAKDDPIYLATRRTILSQENP
                     YYYQGNAAAGIGSSHTPENYIWHIALALQGLTALDQDSKKEMLDLLVATDAGTHLMHE
                     GFDVNDPYQYTREWFSWANMMFCELLLDYLGFSITP"
     gene            23649..26354
                     /locus_tag="VC122_000755"
     CDS             23649..26354
                     /locus_tag="VC122_000755"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014635601.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-mannosidase"
                     /protein_id="HEP5640698.1"
                     /translation="MATKKVHIISHSHWDREWYMAYEQHHMRLINLIDDLLEVFQTDP
                     DFHSFHLDGQTIILDDYLQVRPEREPEIRQAIASGKLRIGPFYILQDDFLTSSESNVR
                     NMLIGKEDCDRWGASVPLGYFPDTFGNMGQTPQLMLKAGLQAAAFGRGIRPTGFNNQV
                     DTSEKYSSQFSEISWQGPDNSRILGLLFANWYSNGNEIPTTEAEARLFWDKKLADAER
                     FASTKHLLMMNGCDHQPVQLDVTKAIALANQLYPDYEFVHSCFEDYLSDLANDLPDNL
                     STVQGEITSQETDGWYTLANTASARIYLKQANTRVSRQLENITEPLAAMAYEVTSTYP
                     HDQLRYAWKTLTQNHPHDSICGCSVDSVHREMINRFEKAYEVGHYLAKEAAQQIAGAV
                     NTSDFPTDSQPFLLFNTSGHSKTSVTELTLTWKKYYFGQRFPKEVYQEAQEYLAGLSQ
                     SFQVIDVSGNTISEADILDTSIAFDYDLPKRTFREPYFAIKVRLRLPITLPAMSWKAL
                     ALQLGNETAPSATVPLYDDSNQCLENEFLKVMIQTDGRLTITDKQSGVSYQDLLRFED
                     CGDIGNEYISRQPTHDQPFYADQGTTKLNIISNTAQVAELEIQQTFAIPISADKLLQA
                     EMEAVIDITERQARRSQEKAELTLTTLIRMEKNNPRLQFTTRFDNQMTNHRLRVLFPT
                     HLKTDHHLADSIFETVKRPNHPDATFWKNPSNPQHQECFVSLFDGENGVTIGNYGLNE
                     YEILPDTNTIAITLLRSVGEMGDWGYFPTPEAQCLGKHSLSYSFESITKQTQFASYWR
                     AQEGQVPILVEQTSHHKGSLRAEHSYVTGADDQVALTAFKRRLADNALITRSYNLSND
                     KTCDFSLSLPNYNAKVTNLLEKDSKQSTPSQLGKAEILTLAWKKQ"
     gene            complement(26455..27810)
                     /gene="rocA"
                     /locus_tag="VC122_000756"
     CDS             complement(26455..27810)
                     /gene="rocA"
                     /locus_tag="VC122_000756"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010922531.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator RocA"
                     /protein_id="HEP5640699.1"
                     /translation="MLEDFLQFLGFIFLDIIEIMLTLKLFSFVSAIPLRLKNIFYLSL
                     SMVLFQVVFWAFFPDHFILDVVMLAQFLFFALIALYYGKSIKAKFLMFYAFFPLVSIS
                     LVKRFIVFFVMPLFGMPYSVVKHNTLLIYSITCFSIFLIYRCIQVFHFDFSTWRQYFQ
                     SHRASKLLVFTNSSMALYYLCVQGIDVMSPSLSGLATTTARSIIVLFYFILFLTLLIH
                     LERYVKQNSIEAIVQQKEYRELINYSQHLGLLYQDIQELRQLLTTVSSRLKIGIEQND
                     ISIVRLTYEGILNAEKNNAKDDRLDLTCLDKLQVEAIRHIVLAKLIEAKNKKLKVEVS
                     IPNCIATFFLEVVDFTKLLSFLLDNAIEMSLETKQPCLSIAFLDQNHKLVIVIQSSTK
                     QGQNDSQSVFATPALKKRDDWQFDLRNVTTILNRYDYLTISSQIHDGILTQLIEIAKP
                     D"
     gene            complement(28475..29830)
                     /gene="rlmD"
                     /locus_tag="VC122_000757"
     CDS             complement(28475..29830)
                     /gene="rlmD"
                     /locus_tag="VC122_000757"
                     /EC_number="2.1.1.190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002988977.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (uracil(1939)-C(5))-methyltransferase
                     RlmD"
                     /protein_id="HEP5640700.1"
                     /translation="MVVKVKQKIPLKIKRMGINGEGIGFYQKTLVFVPGALKGEDIFC
                     QITSVKRNFAEAKLLMVNKASKNRVKPACSVYETCGGCQIMHLAYPKQLDFKDDVIRQ
                     ALKKFKPTGYEQFEIRPTLGMKKPDHYRAKLQFQLRSFGGTVKAGLFSQGSHRLVPID
                     NCLVQDQLTQDIINKITQLVDKYKLPIYNERKIAGIRTIMVRKAQASDQVQIIVVSSK
                     EVRLANFIGELTKAFPQVKTVALNSNRSKSSEIYGDETEILWGQEAIHEEVLDYGFAL
                     SPRAFYQLNPQQTEVLYGEVVKALDVGSKDHIIDAYCGVGSIGFAFAGKVKSVRGMDI
                     IPEAIEDAQKNAKAMGFDNAYYEAGKAEDIIPKWYKQGYRADAIIVDPPRTGLDDKLL
                     KTILHYQPKQMVYVSCNTSTLARDLVQLTKVYDVHYIQSVDMFPHTARTEAVVKLQKR
                     V"
     gene            29945..30721
                     /gene="recX"
                     /locus_tag="VC122_000758"
     CDS             29945..30721
                     /gene="recX"
                     /locus_tag="VC122_000758"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009880741.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombination regulator RecX"
                     /protein_id="HEP5640701.1"
                     /translation="MKITKIEKKKRLYLIELDNDDSLYVTEDTIVRFMLSKDKVLDND
                     QLEDMKHFAQLSYGKNLALYFLSFQQRSNKQVADYLRKHEIEEHIIADIITQLQEEQW
                     IDDTKLADTYIRQNQLNGDKGPQVLKQKLLQKGIASHDIDPILSQTDFSQLAQKVSQK
                     LFDKYQEKLPPKALKDKITQALLTKGFSYDLAKHSLNHLNFDQDNQEIEDLLDKELDK
                     QYRKLSRKYDGYTLKQKLYQALYRKGYNSDDINCKLRNYL"
     gene            30801..31334
                     /locus_tag="VC122_000759"
     CDS             30801..31334
                     /locus_tag="VC122_000759"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002949762.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF402 domain-containing protein"
                     /protein_id="HEP5640702.1"
                     /translation="MKLPKEGDFITIQSYKHDGSLHRTWRDTMVLKTTENALIGVNDH
                     TLVTESDGRRWVTREPAIVYFHKKYWFNIIAMIRDNGVSYYCNLASPYMMDTEALKYI
                     DYDLDVKVFADGEKRLLDVDEYEIHKKEMQYSADMDFILKENVKILVDWINHEKGPFS
                     KAYITIWYKRYLELKNR"
     gene            31755..32118
                     /locus_tag="VC122_000760"
                     /pseudo
     CDS             31755..32118
                     /locus_tag="VC122_000760"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_086558053.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
BASE COUNT         9856 a         7017 c         6065 g         9472 t
ORIGIN      
        1 ttaaaaacta taagaaaatt tctaattatt cccggtggtg gcatctataa aaaatactgt
       61 cctaataaga tttggggcag tattttttta tagctagcaa gcctttttta ataaaaatga
      121 ctaagaacat agacaatcta gggtttactt gctatttcat cttgtttatg gtatcatttc
      181 acaggctaag ggtgcttcat ggataacaac gagattttgt attcctaaat aagacgttat
      241 cccatagagc acgccataca gaattagtaa aggaatcaaa cgaatgattg acattatgtc
      301 tgactttcaa aataagactt gttctaagaa taattttatt gttggacctt gttccattga
      361 atcttatgat cacattagat tagcagcttc aagtgccaaa aaacttggtt acaactattt
      421 tagaggaggc gcctataaac caagaacatc tgctgcttct tttcaaggcc ttggcctgca
      481 aggaattcgt tacttgcatg aggtttgtca ggaatttggc ttactatcgg tcagtgaaat
      541 catgtccgag cgccaattag aagaagccta tgactatctc gatgtcattc aggttggtgc
      601 ccgcaacatg caaaattttg aatttttaaa aaccttgtca cacatcgata agcctatttt
      661 gtttaaacga ggcttgatgg ctactatcga ggaatatttg ggcgcccttt cctacttaca
      721 agacactgga aaatctaaca ttatcttatg cgaacgtggc gttcgtggct acgatgtgga
      781 gactagaaat atgcttgata ttatggcagt ccctattatt caacaaaaga cagatttacc
      841 gattattgtg gacgtgtctc attcgacagg cagacgagat ttactcttac ctgctgctaa
      901 gatagcgaag gctgtcggtg ccaatggcat catgatggag gttcatcctg atcccgacca
      961 tgcgctgtca gatgctgccc agcaaattga ttataagcaa ttagaacagt tggggcagga
     1021 actttggcaa gattaagctc taaatatcac tgcgttatct ctgttaccta gtactgatga
     1081 aaggagtgct tatgccacaa acacttcatg tccattctcg ggtcaaggat tatgatatcc
     1141 tgtttaccga ccatgtttta aaaaccttag cagattgcct tggtgagcgc aagcagcgta
     1201 aactcttgtt tatcaccgat caaacggtct accacttgta tcagactctc tttgaagaat
     1261 ttgctcagca atacaacgct tttgtccatg tctgccctcc gggaggtcaa tccaaatcct
     1321 tggaacgtgt cagtgctatc tacgaccagc tcatcgctga aaatttttcc aaaaaagata
     1381 tgattgttac tattggtggt ggtgttgttg gggatctggg tggttttgtc gctgcaacct
     1441 attaccgtgg tattccctac attcaaatcc caacgacttt acttagtcag gtggatagca
     1501 gtatcggggg caaagttgga gtccatttta aaggtctaac caatatgatt ggcagcattt
     1561 accctccaga agctatcatt atttcaacca cctttttgga gaccttaccg caacgggaat
     1621 tctcctgcgg catcagtgag atgctcaaaa tcggcttcat tcatgataga ccgctttttc
     1681 agcagttacg tgattttcaa aaagaaaccg ataagcaggg acttgaacgt ctgatttacc
     1741 aatccatttc caataaaaaa cggattgttg aacaagatga atttgaaaat ggtttgcgca
     1801 tgtctttaaa ttttgggcat accttgggcc acgcgattga atcgctctgt caccatgatt
     1861 tttaccatca cggtgaagcc attgctatcg gtatggtggt tgatgctaaa ctggctgttt
     1921 ccaaaggact cttgccaaaa gaggacttgg atagcctcct tcaagttttt gaacgttacc
     1981 agttaccaac aacacttgaa agagctgatg tctctgcgac atccctcttt gacgtcttta
     2041 aaacagataa gaagaattct gagcaacata ttatctttat attacccaca gaaaccggtt
     2101 ttaccaccct agccattaac aaagacgatc accaatttgt cgagaagctc gacagtttat
     2161 tataaatagt gactccactc tagctagtta agcttgctaa gagaataacg gtatcctgga
     2221 actcctaaaa catgttacta caagacaagt cttgataaca atataacttt tagctaagaa
     2281 taactaacag caacatcaaa aagaggattt atggaactat aggagaacgg tatcactcgt
     2341 aaaaagatct ttcttttgcc tgtcgttcaa ttatttcgtc taaaaagcct taatgagaaa
     2401 cctataagga gataggaaac tcattaaggt tttttagtga attctaaaat aaaccaagct
     2461 aaagggctta atctggagat gagaaggcca agctttaaaa ttcgtattgt catcgctttg
     2521 aatacgtaaa aagagaagat gagcgtcatc ttctcttttg cttttccata ggccacccaa
     2581 tacagttgac aataagccct taatctaaaa gcacttgtaa tagtttacaa tcggttttag
     2641 cttctaaatg atgggctttt tgggcaggta tggctatgag actttcttga gttaggacct
     2701 gtttttgatc ctctaaatcg aaaatgagct gaccttctaa aacaagtatt actttatctc
     2761 ttggactagt ctcttgacta atgctttctc cttgggccat ggcatataaa acaatagatt
     2821 gagataatcc cagtagacgg ctcaaggatc ggctagccac ctgatgatca tgatattgca
     2881 ccagttgaga gagattttca atcatgttaa gcctccggtt ttactactac taaaagcatt
     2941 tgaaaggctt ctactgcata taaggcatga ggaatagcag ctggcatcac aatggtttga
     3001 ccttcagcaa cccggtaggt ctcttgatca atggtgattt ctgctaagcc acttaagata
     3061 gtaaccatgg catctcctgg tgatgagtgt cgcccaattt cttggccctt atctaaggaa
     3121 aaaactgtta ttcctaggtc ttgcctttgg actagtgttc tacttaacat ttgatcttgc
     3181 tcaatcatta cttcttgcgt caaatcaaga accttcgcgt gctcaatatt attgatataa
     3241 gacatcttcg aactcctcta tggtctgttt agttgtatgg acaggttggt ctaaggttta
     3301 ttttacactt tttacagcaa tatagcagag atttttgcct gttctggtaa aaaaacgacg
     3361 catctttaaa aactgttgtc tgttctcttt cttaaggcct cgtctcataa tgttcagggt
     3421 gttcataaga ccttcatcct taatcattcc acgaggtgtc atcaaagaca ttttcccatg
     3481 gtgataagtc acttgagaaa aaccgtgttc ttgatataag gcttgccaac ctgttagtga
     3541 caagggagca acattgacat gaatcgtttg acgtaactcg tcaagtactt gtcttgtttt
     3601 tggatcttca taagctacgt catgtgtcaa aagtcgcccg cctggtttta acacacgcca
     3661 atattcattg agtgccttct ccttggcttt ggcgttcaac atggttaaca tggcttcatt
     3721 aatcacaata tcaaaatggt tatctggaaa tggtagtgat aaagcattgg cttgggatac
     3781 tgtaatgtga cgggttaacc cctcaacagt aaccctttct tgtgcttctt tgacaacctt
     3841 ggggtttaag tcaacggcat caatctgaca accgaaggtt ttggctaact ctattgctgt
     3901 cgtgcagcga ttgcaggcaa cctctaaaac gtgacaatcc tgagagaatt gtccttgttc
     3961 aatcaaccag tttgtcgcct caacaccgcc aggtcttaag cgtttttttc ctagacgtgc
     4021 taaaaagtca tgcccaactt cctttgttac cattgctatt ctcctttatt cattgaactt
     4081 ttccttttat aagagcttct tttctaaaaa aacggtaggt tatctggcag acctcaaagg
     4141 aactatgact tgtatcacgt tagggataag gctcttcaaa taagcttttt actcttaaaa
     4201 acagctctcg caaaaagaat tttagcagcc catgatgact gctccatctc ttattaatat
     4261 atcataatta agcttactta aaaagttctt taaaaaaggt tgagttactc atcacctaaa
     4321 agaggtttaa tagcgtcaac tggcatgtct tgaccagtga ttaatttaaa agcttctgct
     4381 ccttgataaa gcaccatccc tagcccatta atgacttttt gggcgccatg ttgacgggca
     4441 aaggctagta acttggtttc tgctgggctg tacacgatgt caaagaccac caagtctggg
     4501 cgaatgagct cggggtcggt aatcaaactt agattttcta aaggcttcat tcctacactt
     4561 gtagcatcaa taaatagatg agattccctg atggcctcct taaaggcagt ctgatcttcc
     4621 aagtcttgaa gggttacttt agtccttgtt agctggttca gttttgtcac aagcttttga
     4681 acgctactta gacgtgtagc ttgtcggtta aacaaacgaa tctctttggc gccgtcaaaa
     4741 gcgagctgga cagcgatagc tttcccagcc ccaccaactc ctgctagggt aatgattttg
     4801 ttctttaccg acactccttc gtcagccaag gctctgagcg ctcctatgcc gtctgtaata
     4861 tgccccacga gatgtccttt accatcctta ttgacaaccg tattgactgc accgaccaac
     4921 tcagcagctg gggacaagtc atccaataag ggtaaaatag cttctttatt tggcatagac
     4981 acattggagc cacgaattcc taaggcacga atcccttgca cggcatctgc taattgctct
     5041 gtccctactt caaaagcaag gtaggcataa tccagcccca gtttagcata ggcttcattg
     5101 tgcattttgg gagacaaact atgacgaata ggagtagcaa gcaaagagac cagcagggtg
     5161 tggcctgata aacgttctga catttagaaa tctcactttc catttcaacc ttgtaaaggc
     5221 gcctttcacg taataaagga cgcctatttg ttttttatta taccttattt tacaaaattt
     5281 atcagacctg gtgccaaaga agtcttcttt tacttatgct tcatgctaaa tcccaaaggc
     5341 caatttgaat actgtcttca gttaggtcag gtccttcaat gacttcaagg gtcactacta
     5401 gatacaaaac agtcaaaata tcatcgaaga aaataacctc ttctaactgg ctcaagtggc
     5461 tatcagctag catcaaggtt tcttgcttaa tagagctctg actaccataa ctgattgcca
     5521 cagaaacact aagagatgtg tctgtgtgat tttgcaagct aatggctcct atggcttgca
     5581 ccgtatcata ttggtagcaa taagttagcc ttttttgact tgtggccagg gattgcttgg
     5641 cgactaaact gagatcctgg tgccgtcttt tgctagcttg tgctaggtta agccttgcct
     5701 caaccggtat tgattgccca aaacaagaaa catagcccgt gacaagaata ggcttggcaa
     5761 agattttctc ttttaggtac catgtcattg gaaaagcttt tttcacaaaa gaaccttggg
     5821 catctttgac ttggagccac tgaggcaaga agatatcgtc gcctttttta aaatcaaatg
     5881 cctgagaaca aacctgcaag ggcagagata aatagtgctg gtaactggtg ataagaatgt
     5941 gaggtgtgct aaccttacct gctttttgct gggtgtcttt gttctccaaa acagaaagga
     6001 attgtgtggt ctttagacaa gattgaccaa tttcttgttg acttgtcagt cggctctttt
     6061 taaactgata tttctctaaa ggtagtgtca ctaagtgttc tttgggggca cggcaggcta
     6121 cttgtgtctg aaaaacactg ctaagttgct gcgagacttt tgccgtgacc ttaatgtgtc
     6181 ccgcctctcc agtgagggct aaaatggcta ataattttcc cccatatgcc tggcgtagag
     6241 gctcctgata aggggtgtgg tcagtcggat tcccattatc aagagccaac aaatgggctg
     6301 gtccttctac ttgaaccttt agccaatctt gagcatggct gaccaattca cctcggctat
     6361 ccaagagtga taaatccaga taaagcaatt cccaaggagc gtcaataggt gcttcaaagg
     6421 ctttccaaga tagctttgcc actgaaccat aggttctaac ctgagaacgt ccactggttt
     6481 ttggaatgct tttaccagaa atgtcgtagg ccactgccct tagcaaccct ttttgatagg
     6541 gcactcgcca agtcaggtaa agattctcgt gaggattctt agcagcgtca gctccttggt
     6601 aaagctgata agtgtgtccc gttggcgtgc tataagtatg aaatgccttc cttccataat
     6661 ctgttacgtt gccctgctca tcttcaaaca tcagttgaac actggcagca ttgctataaa
     6721 ccacaacctc caccaatccc tgctcatcga aacaaagctg gtccttctgc cagactggta
     6781 acaggtgcaa ggtggtctgc ccctgcgccc actgactttg gtaaaaataa tagctatctt
     6841 ttggaaagcc tgccgtgtct aaaatgccaa agtaggcatt tttaggagaa ggccaaaggc
     6901 ctacaacacc gctgtcgatc ttgttccagg gtgtaggttc tcctaaataa tcaaagcctg
     6961 tccaaacgca ttcgccagca acaaaatcac gggtaatggt atcataccaa gcctggctag
     7021 cgaatgcacc ccaatcgacc ttagcatgat cataagaggt taggtgataa ccggagtcaa
     7081 gaactttttt tgtctcttta taataagccc tgctggtaat ggctgaggct gtttcagaac
     7141 catacaacag ccaatcagag tgctcctcat gaagcttatc atagtctttt ccattagcat
     7201 agtttaaccc aatgagtccc tggggatggt ctaattgact aagaagagtt gccatttgcg
     7261 acacttcctc atgccagcaa aagtcagctt cttttaattt attgtcccca aaggtaatgg
     7321 gacgtgaggt gtcaatagca gcaatccatt ggcacatttg tcttgttagc tctgggtaat
     7381 gactgacatc ggctgaaaaa ccttccatca attcattccc aatcgaccac atcaacactg
     7441 acgggtcatt cttggcagac cagaccatgg cctcaatact atactgggcc cagctcgttt
     7501 caggtgacct cactctttga agatagcttg cattttcagt tccgatggtc tgatggaaat
     7561 agtttgaaaa gtcattaaca tttccatttt tggcataggt ccaggtgtca aaggcttctt
     7621 caatcacaaa aaagccgaga cgattggcta gttgcctaag tttgggacta cttgggttat
     7681 gggtgctacg aatggtatta gcccccatgt ctttcaacag gactaattgt ctggctaggg
     7741 catcttcgta agcacaagct cctaggcctc cttggtcatg atgcaaacaa actcccttta
     7801 atttgacagc tctgccattc acaaaaagcc cctgattggc cataaaagtc agctgacgaa
     7861 aaccagtttc aaggcaaaag caatcaatga cctgaccttg atagtaaaga gtgagttcta
     7921 aatcgtagag gtgaggatta tctggtgacc acaattggag ttcttccaaa gacagtgtaa
     7981 gaccatactg tctttggaaa gccaaatcag ctagaggcac ttctgtttct tgataaagga
     8041 gtttcttgtc ttttgagcaa tgtgattgtt cccataaaca gaggctcaat tggtagtggc
     8101 aagtctgaat agactgattg agggcaaagc gtaaatcaac tttctgttgc cctttctcct
     8161 gcacagcagt atcagccaag gtcattgcta cctggtctgc cacaaaatga agctggggaa
     8221 gaacagacaa actcacttca cgataaagac cactgcctga ataccaacga gaactgggaa
     8281 ccttgttttc aacccggaca gccagaagat tttcagctcc aaaagtcaca acatcagaga
     8341 tatcataagt aaattcctga tagccattta agtgtttgcc aatgaattgc ccgttaacat
     8401 aaatttccgt ttccatatag gaaccctcaa aagtaatggc aacatgacaa ccagctaaaa
     8461 cctcatctaa cacgagataa tgccgatacc aaccgacacc acctaattta taagcacttt
     8521 cagcttcacc atttcttgtg taaggctgcg taagactgaa atcatgtggt aaatgaatca
     8581 cctgccagtt cgaatcatca aaagaagggt cctgagcaca cgccacatct gccataagaa
     8641 aacgccagcc ttgattaaag ggctgctttc ttccttcttg atcaatatag tggtattggt
     8701 agccagtcac tgtctgctcc ttgctatctg gtgtcatatc aatctcctgt ttgacgttac
     8761 tggtcgccct tgatggcttg gtaattgtcc ttgtaacgat ctctgaactc ttttggcgat
     8821 aaaccattca actttttaaa catcttgata aaataatgat tggtattgta acctgtttca
     8881 taagcaattt cattgatgga ttggttggtg taaagcaata actgttgcgc tttttgaatc
     8941 ctaacatgat ttaagtactg ggtaaaacta cgctcggttt catttttaaa gcattgccct
     9001 aaataaaccc cattgacatg taacctatca gcgatagctt taagggttaa ttcttgatgg
     9061 tattccttgc gaatgcaatc aatggtttca ctaactaaat ctgaataacg tttttcaggg
     9121 gaattgttct gattggtgac atccaacact ttttttagtt ccctcaaaat gcgatcaatg
     9181 gattggctgt tatgaatagt ttttaccatg gacagataag tcattttatc taaaatggga
     9241 aattgacggt aaatgtcaga gaacaacaag aacgacacat gcttcacatc ctctggtgag
     9301 aagttcaagg ccttcatttc atgaaaaatg gcttctaatt tggccaagat ggttggctca
     9361 tcaccaatca tcaatgcttt gttaaaggca aaaaattgca accgagattc tggcatctca
     9421 agcttactgt tgcctttgtc agttggttgt gacatttggc tattgtaaaa aagactctgg
     9481 cgcacctgat tgtagctctc ataaacattt tcccagtcat caaccgtttc tcctaggatg
     9541 atttgttgga tgttctctga aaattgcttt tgtagattac tggtaaaggc tttggcttgc
     9601 tgggctctcc caagtaaaat cgccaagtaa atcttcttgt cttcttcttt tttgaggtaa
     9661 cagggttgtt cttcttgctt aaaataagcc tccatctctt ctatccactc ggaacactcc
     9721 acatacagca cggtaaatcc tgttagggca tgctcgggga gatcctggct aagttgatga
     9781 aattcttttt cactaagttc atctgttaac cacagtgtca gaaggctgtc atgataggtt
     9841 tcttgccgga ttgattcagc ctgctgggca tccaaccagt ctttaaattg tttcatttta
     9901 gcctgcaatt ctgccttatc cacaggtttt agcaaatacc ccttggtttc taattccata
     9961 gccttttgca cataggagaa ttcttgataa ccagataaaa tcaaggtttg tagctgagga
    10021 tgattagttt tagctgcttc tattaaatcc agtcctgtca ttccaggcat ggtaacatct
    10081 gaaatcatga catccactgg gtactgagtc aaataggcta aggcttcctg gcctgatctt
    10141 gctgttgaaa cgacgtcaaa accaagagcc tgccaatcaa taatcatggt taatccttgt
    10201 agaatcatgt attcatcgtc aactaataat accttataca ttaaacatcc tttctaatcc
    10261 taatttgaat gataaagcca ccatttggct ccttagccat ggaccaagaa accctgtctc
    10321 gaaaatgatg gaaaagacgg aggtaaacat tttgcaaacc aatagagcta ttgcctgttg
    10381 tttggtgctc ttgaagcctt ttttccatat ctgctaagcg ctcttgacta ataccacgtc
    10441 cattatcaag cacctgaatc aagagatgat ccgtctcatc taatgccttg atgcttagtg
    10501 cattgtcatg gcgactataa tcaatcccat gcacaaaata attttcaacg agaggttgaa
    10561 tgacaaattt aggaatggct aaatcagcaa tactctcatc aatttttaca tgataagcga
    10621 agctatctgg ataccgcatt tggtagaggt aaatgtactt ttcacaaaaa gccagttctt
    10681 cttttaaggt ggtcatctta tcttggctaa tattgttgcg aagcaaactc gcaaaggcat
    10741 aaatgacatc tgctaattct tcttgttgac aactaagggc gtacatccga atatactcta
    10801 acgtgttgta taaaaaatga gggttgattt gagactgcag agctctcatt tgggcatctc
    10861 gttgctctac ctctaacaca taaacttcgt caatataagc tttaatgctg gccaacatct
    10921 gattaatggc ttctgagata tgatacagtt ccaattccat gtggctgttg tcgatggtta
    10981 aagacaaatc gcctgctgca accatttcaa cagtatccgt aatttcagaa acctgctgga
    11041 tataatttcg gaaaacccta aagagcaact gcaataacag ccaagctaag agtagagaaa
    11101 caaggccata cacaaaaact gtcctgacag cttccgctaa taaataggat tttctgacag
    11161 ccacataagc taagaggtct cccgagcgat tgtactgcat ccgataagcc ttgctgagat
    11221 tactaagatc agctgcccga tctgccttga tagccttttt aacctcagcc tctaaatgag
    11281 gaaatctccg acctccttga tggaagagtt tgcggccata gtaatcaaaa gcaaaagtag
    11341 ctaaatagtg atcctgctgt gtctctacca aagtcggtgg tttgtcaaag gtcaggtaaa
    11401 gacgacctgt cacatctcct gattctggat ttcgaatgga gcgaactaaa acatttccgg
    11461 ttactttttc aggcttgcta taaagcaaac gacccgtctt aatgacccta tcggcgtaca
    11521 gataagcact actgtcctct agcagtaggg tcatagaagt cacatcaggg tacaagtcaa
    11581 aaagacgcct cacctcttca gaaaatagga ccgtgtcttt tcctttttcc cagtcttcaa
    11641 aaacgtttga aaaatattgg tttggggtta acgtcatata ttgatagagg ttatggtaat
    11701 cttctgaacg gccaaccaac tggtctaaaa cccgtagggt aatatcgttt tgtaactgca
    11761 atctacctag agtctcctcc agtcgcatct gcgcctcagc atccaccctt ttataagtac
    11821 tactaataga ataactgctc aagccaatag ctgctaaagt gaaaaaactt acaattagca
    11881 ggctatataa tttcatcagt cgattaatca aaaaaccctg cttaaagtgg ttagtcacat
    11941 accttcccaa tgttttaaca ctcatcaatc actccatcta ctagtcgacg attagcctta
    12001 gtgacaaagt ccaagcaaaa gactagagct ccgaaggtta aaaagagata aaggcctttg
    12061 tattgccaag tcattaaggt aattcccagc aaaacactca gcatgagtaa aaaggatttg
    12121 aaacttaaaa agacgctgat aaaggctaat ttcatattat tccaaaggtt aatctcatag
    12181 ctcactgcat aaaaaaccat gtaaagatag gtaatggtta gccaaaccat ggctattaca
    12241 atcaagaaag acacggtaaa ccaaaacaag gatggaaact gtactgccca atgcaaatta
    12301 aaggttaaaa aaaggaaagt cagggcaaaa agaccaaatc ttagattagc ttctttgaaa
    12361 taagatttcc aaagttgaac agcccttttc aaagaatagt ggttttcttc ttgaggtgtt
    12421 tttaaaaata gggtgacaat ggtttgccag gctggcccca ccccaaacac aatcagtcca
    12481 gctaaggaca agagatgaaa aatgagggtc aattggatca ttttccaaat taaaaagcag
    12541 gttttttcaa tgccattcat atgatagtcc tctttttctt gttaacactt atagcggttt
    12601 caaggaccag cagctacgag actagtttta gcacccccag taagatgctg gtattagttt
    12661 tactctgatt aatagtttag actcttctaa tatagcacac tttataggct tttggaaacc
    12721 gctttactaa gagtagtaac attatttaca ttaatttttc tccctttata acttccactt
    12781 atttgatgat ccgttttttc cactcatcag aagaaaagag ctgagatttc ccagccctca
    12841 acttataatc aagaaaatga atgccttact tgtctttagc tacaaagtcg tccaattgtt
    12901 tttgaacttc tttttgcacc ttgtcccaac cagcaccttt gaggtctgca agaagttttg
    12961 gaagggcttc gtcaggatcc actgtaccag tattgatact tgctttatat cggttcatga
    13021 catttgaaat atttgataat tccgttttga taacttttgt gtcaacagtg aaaccaagga
    13081 ttggagattc tttagcatcc ttgattgatt ggtcacgttt agcaatcatg tcgtcagtaa
    13141 cagattcttg agtgtaaagg attttattgt taccagtatt ccaagcaccc atgtgcattt
    13201 ttggttgata gccatcgagc agtttgattt tcttgtcgcc aattttttcc caagctttgc
    13261 cttcgacacc ataaacaagc ccatttaaca attcgggatc gctattaaga agagaaagga
    13321 cttcaactgc tttttctttg tttttagata cgcttgatac cacaaagttt gccatttgtg
    13381 cttgtgatgt ggtttttagc ggtttagtca atggacgaga cacgatgtct tttcctgcag
    13441 cattggtcaa gatagtatcg ccatagtcca taggaccttg ggtttcttca cgcataaacc
    13501 aagtgtttcc ttcaagggga taaccttctg tattggtcgc tgcatctgtt ggaatcaaac
    13561 cttctttata ccatttgtgc atcaagcgga gattgttttt aaaggactca tcttcatact
    13621 gattaatgat ggttggcttg ccttcatcaa ttttcacagc aaagggttgg gttttggtta
    13681 atgggtagtc atagtcacct gacatactaa agacttgacc aatagcaaaa gctgctgtat
    13741 ttggttcttt ttcgtggaat tgtttcaaga cattttcagc atctgcatag gacttaatgt
    13801 ttgaaatgtc aaggccatat ttgtccacta gttctttatt gaaagaaagc atctgttggg
    13861 cataaacgtt ggcatctact gggaaggcat agagtttacc gtcaatagta tttcctttaa
    13921 tataggctgg gtctaagttt ttatacgttt tcttagcgta ttttggcatt aacgttgtca
    13981 aatcagcaaa agcacctttt tgtgcattga ccacataatt attggcaaaa gcaatgtcat
    14041 agttttcacc agaggcaatg atggtactca ttttatcatc ccagtccccc caaccgatgt
    14101 attgaaggtc aaccgttgca cctgtttttt ctttgatgcg cttgttagca attgtcatca
    14161 attcatcgaa gttatcaggt ttgtcaccaa cttggtacat caataatttg acatcactat
    14221 cctttgatgc tgacttgctt tcgcaggctg ctaaactaga cgctgcaagc acagttccag
    14281 tcacacacac gattttttgc cactttttca taagtgaagc ctcctaaaaa atgttttctt
    14341 ttgttactaa tgattagata aaatctggtc ttgtgaccag ttccttaagt cctattcctt
    14401 caccccacca atggtcaaac caccgacaaa gtacttttgg aagaatgggt aagtcaaggc
    14461 aatcggcaag gttgcaataa ccacgatagc catacgaact gattcttttg gcagcgatgc
    14521 taagctatct gccatttgtg cactcatgcc agcattttgt gccaaggcct gtaggttgct
    14581 ctgaattttc atcagtaagt actgcattgg gtagaggttt tcactttgca cataaagcaa
    14641 ggcgttaaac caatcattcc aataagttaa ggcagaaaat aaactgatgg tagcaatccc
    14701 tggaacagcc aaagggagaa caattttcat aaaaatcatc cattcactag ccccatccat
    14761 acgagccgac tcaataatgc tatctggaac tgtcttttta aagaacgttc tcatgaccaa
    14821 gatgccaaat gggcccagag ccattggtaa aatcaaagcc caaaccgtat ccttcaaatg
    14881 aaggagatta ctgaccacca agtaattagc aaccatccct ggtgcaaaga gcatggtgat
    14941 aagtgcataa acagtaaaaa aacgtctgta tgggaaataa ggtcttgaaa tcgcataagc
    15001 ataggttgat gtcatggtcg tgttaataaa ggttcccacc actgtaataa acacggatat
    15061 cccaaatgac ctcatgattc tatgggacat ggctccttga aaaatatact ggtaagcctt
    15121 agttgaccag acatctggaa aaaaactata cccatgatta atcaagtaac tttcatcagt
    15181 gaaggaaata ataatcacga agataaaagg aatgatacaa gagatggcaa acagaccaat
    15241 cagaatatta aagatagcat tggtttttcg atcaaaggtt ctaacattaa ccttttcgac
    15301 ttttttcttt ttctccatgt tttcctccta aaacaaggct gcatcaggat ccatgcgacg
    15361 aacaatcgca ttagtgacta acaagatgct ggtcccaact actgactgat aaaggctagc
    15421 tgccgatgcc atcccaatat ctcctgtagc agtcaaggca ttgtagacat aggtatctaa
    15481 cacactggtc acgtcataaa gtgggcctga attcttggga ataacgtaga aaagtccgaa
    15541 atcagattta aaaatattac caatattaat gatcaataag atagacatca ttggcaataa
    15601 ttgtggaatg gtgatgttcc taatttgttg ccatttgctt gcaccatcca ccatagcagc
    15661 ttcataataa gtatcagaaa tccctttgac tgaagcatag tagataatgc tattgtagcc
    15721 tagccctttc cagccactca taaagagtaa aatcaagggc caccaagtgg gattggaata
    15781 ccacttaatt ggatctgctc cactgccaac caagagctga ttaatgaaac ctttatctgt
    15841 atttaaaaag gcagaaacaa agtattcaat aaccacccat gataagaaat aaggcaacaa
    15901 ggacatggtg tgatagacct taaccgttcg cttatttcgt aactcactca tgataatggc
    15961 aaaaacaatc gcaaagaaaa cattaaaaag taagaaaatc acattgtaag cgatggtatt
    16021 tctggtaatc aaccaagcat ctttggaagc aaagagatat ttgaaattag ccaaaccaac
    16081 ccaagggctc tccttcaaac tttccataaa gccccctgct gaataatgaa aatctttaaa
    16141 agcaaccaca ttggctaaaa ctggaatata aaagaaaaag atgaaccaga tgaaacctgg
    16201 taataccatt aataataagg cccggtattt aatcacattt ttccaaaagg atgtcttttt
    16261 gttggtttgt cttgtcattt cccaacctcc ctttgtgtta atcacgttat agttacagta
    16321 tatcaagcgc tttcattatt ttaaagcaac aaattatagg tatgataaca caaagtatag
    16381 aatcacttag aaagcctttt cattgttgct taaatctgtc atactaagat gagacatgat
    16441 cgtctattct actataataa aggagaacac ctcatgagct tgttatgtat tgatatagga
    16501 ggcacttccc ttaaatttgc cctctgtcac aatggccaat taagccagca aagcagcttt
    16561 cccactccct ctagtctgga aaagttttac caattactcg accaagaagt tgctcgttac
    16621 tctgcttacc atttctcagg tattgccatc agttcacctg gggcaatcaa taaagaaaag
    16681 ggggttatcg aaggcgccag cgctattccc tacattcacc attttaaaat acaagaggcc
    16741 ttagaagaac ggctacatta ccctatttct atcgaaaatg atgccaattg tgccgcctta
    16801 gctgaagcta ccttaggggc aggaaaaggc gctagtagcc tagccatgct cgttcttggt
    16861 actggagttg gaggaagctt ggtcattgat ggcaagattt accatggtgc ccacttattt
    16921 gggggcgaat ttggtttcat gatcatgaac gaccgctacc aaacctttag tcagttaggg
    16981 acagtggtaa atatggccaa gcgctatagt gcaatcgtca ataacggaaa agattatact
    17041 ggcaaagctg tccttgccct cgcagaacaa ggagaccacc tagcactcaa agaaaggcag
    17101 gtctttctcc aaagtttagc catcggtatt tttaacattc aacacgcctt tgatcctcaa
    17161 ctgattttaa tcgggggtgg tgtttcccaa gctgactttc tactgtcagc gattgaagct
    17221 gaactagaca aactctacca agccgtcgaa atctccgacc ttagacccca attagctatt
    17281 tgtcacttta aaaacgaagc caatcttctt ggagctgcca ttgattttta ccaagaacac
    17341 aaggaggcct aaccatggac cgctaaatcc ctcttatccc ctattgtccc taatcgcctc
    17401 tgacatctac aaagacatcc taaaaggatt gctgattagc tacaacgata ctcgctttct
    17461 gcctaaatga ctctctgctg atgaacacag cctgatgcct ggtccctttg ttgatgtcat
    17521 tgttgctgac gcttctgtta aaggcattgg tcatcagtta atactcgagc tattagaagc
    17581 tatgctagtt atcgccaata agcaaagcct cacttaataa ctcactcata tagtcccctt
    17641 atcgtggaca gtgacataac aaacagtcac tagaaaccat gaggggcttt tatctggtcc
    17701 aaaaatatga aagaaaatcc taaaatacca agatgaaaaa caagcatcct tagccaagat
    17761 actctaaaga attgaagagc caaattaccc aagatggata gatgatgcca aaccagacag
    17821 aacgcttata tctagttcgg caaataccaa caccttccaa agaaatacaa gagcctcgat
    17881 cctcttaaca tgaggaaaaa ggcccttgtt taatcattgg taacatcatt attcacttgt
    17941 ctcaaaggag acgttcacac gttcaatcac tgaggtgaga agcagagcct ccaaactcct
    18001 ccattacttc taatgaaatc aagcggttat gagtagctac ttttttaaac caataagctg
    18061 aagctttagg tcgtcgcact tgggtatgga tattattttc cacaaggcca tagcggtttt
    18121 tataggcatt gagccaagac cagccatcaa ttggcgtcca tacatggtat ccaaaacaat
    18181 tcgagcctgc ttctattcct ttgtggaggt aggtcaaatg ttctttcaaa aatcggatgc
    18241 gataatcatc ttgaatctga ccagtttcat cacggtaacg gtcttctcct gaaatcccta
    18301 caccattttc agataagaac caaggaatat tgtcgtaatg gtctcgcatc ttgatggcaa
    18361 tatcatagac agcctcagga tagatttccc accccttatc cacgttcatg cggcggccag
    18421 gcatcaggta ggcgtcataa taccattcag gactccaaga tgggctaatc actggaatga
    18481 catccggagc cttcacgcgc ttagggtggt aaaaattcaa tcccaaataa tccactctat
    18541 tctcagcaat aagagccaat tcttctggcg ttgactgcca caaaacccca tcttttttca
    18601 ataccgcaac caattcttct gggaatttcc catgaacagc agcttccata aacaagtcat
    18661 tgttccaaag ttctgcaaaa tgcgcagcag ccatgtccgc ttctgattga ctagctgggt
    18721 aggctggcgt cagattgaga atggttccaa tacgcccatc actaagttct gctggacctt
    18781 gcctgtaagc ttgaataacc ttggctgtgg ctagggctag gttataggcg acctggactg
    18841 cttttttccc atccacgata gcagggtaat gaaattgcat taaataagag ccttccacaa
    18901 cgaccatagg ttcattgtgg acaaaccaat ccttaacccg atcaccaaac tgctcaaaac
    18961 aaaccttgct aaaggcgaca aataagtcca cgacatgctt tgattcccag ccaccatagg
    19021 cttgatagag ggcaatgggc aggtcaaaat gatgcaaatt aatgacagga cgaataccat
    19081 tggccaagca agcatcaatc actcgattat aataagcaag gccatcagga ttaatcgtca
    19141 cttgctcaaa gtcatcaatc aaacgcgtcc actgaatgga ggtgcggtag gaattgtgac
    19201 ctagtgaagc taacaaggcc aaatcagatt caatctgatg ataggcatcc gaagccgtat
    19261 caggtcccac gtaatcgtaa aacaagtcag gctcctcttc ataccaataa tcaaagagat
    19321 tgcggtgctg tttagcaaat cgcccctcac tttgagggcc tgaagtggct cctccccacc
    19381 aaaactcttt tggaaatgct aacatatcat tcctttctag ctcgtatgac taagcataat
    19441 cttcaggtct tgcttgggta acttgacctt gattagctgt tagtttatct tgctgttgat
    19501 agtagatgcg atagtagcga cttggaatgt cacttccaat acggtgttta tcttcttgta
    19561 agtcagcata gagtgtttcc aaatgaggcc cctgctctaa aatggcctgc caaaaatctt
    19621 ggtctttggc cacacgttca aaccaaggaa caatagcttg ttgaaacgct ggcttatcta
    19681 gctttctcaa cgtattgaca cgttctacca attcatctac ccaagctgat aacgttgata
    19741 catccttagc caggagagct tcttccactt caaaaggaag gtccctgtgc agatgacgat
    19801 tgccattatg ccaagcaaat gcctgtaaag ctaaggctaa ttcagcatct cctgtatagt
    19861 ctgttaaggt ttctagccaa gagttactag gctgataacg gttagcatcc cacagataat
    19921 ggctcatgtc tgtcaaggtc agcttagata attcccaact aatcataggg ttagaaacaa
    19981 tccccttgac ctgatagtct gattggcata aaaatggtga gcgattagca aaaggggtta
    20041 aaaagagacg ttctggatct ttttgataat cattaacagg aatattgtcc caaataatga
    20101 taggccgctg atatactgct gccatggttt cgatatctgc ttgcgagatt tgactcgcta
    20161 gcgtgcttgg gcctgtccag aaaaaggcga cttctttagg aatcctttct gacagctctt
    20221 gcaaatagat agaatcatgg tggttgtcgt attctgtcgg acagatcacc aattctggcg
    20281 ccgcatgttg ttggtttaaa aagtgatgaa cctcagttgc caagtgggcc tgggcatagg
    20341 cagtcttttt gaatcgtctc tcaacagcat ctacaatttg gtagtcaata tcatccagca
    20401 aaagcccaaa atgacaaaca cctaacgcta gaagttgttg caatttttga tagagcaact
    20461 gatagtcagc ttccttggta taatcaaaat caagtcccgg actaatcata taccaaaaat
    20521 caatcccttc ttccttggct actgcaagta gctctttgaa ataagtcacc caatcctcag
    20581 gataaagatc gcgccacagt tttcgctggt aatcatcatc tttcggagca tacatgtaag
    20641 tattcatcct tttacgtcca atgaaacgca ggcaatctaa ccgctcttct cgtgtccacg
    20701 gtgttccgta aaagccttca attatccccc gcatagcaaa agaaggacta tgcttgacag
    20761 atacaatcgg taaataaagt ttatcccctt tgaaagtcat cactttaagc aaagcttctt
    20821 gggcgtaacg aaaacctcta aggttttggc tttcaataat aatttcctta tcttctgaaa
    20881 ccttcagggt aaaggcatcc ttatccagct gacggttaaa gaaatgttta acggtcaact
    20941 ctgcttggcc ttcttcaatc cactgaaaat actttgacgt tgccaattct gccagtaggg
    21001 ctttttgatt ttcaaaacgg aaaaaacggc caataaagcg gcatgtcaac ggcatgtcaa
    21061 agacaagata gtctcctctt gtcatgactt cttgataatc ctttaaggcg tgataaatgg
    21121 tcatttctgg tctcctttac ctgagctaac cagctcatgc tagtaacgtc ttttttagtg
    21181 actactttct ctgataaata cttgaacagg taactggcaa gacaccactg cttggtctgg
    21241 ctcatgaata atctgcaaca agactttagc tgcttcttgc cccatttggt aaaaatcttg
    21301 agctgctgtg cttaatttgg gactcaacaa acgggaggct tgactattat caaagcctac
    21361 aatagcgact tgatcaggaa tatataagcc tacctgtgtg attttttgaa tcagctgaat
    21421 agccaaccaa tcgttttcca cgacaagccc ccttttagtg gctgtcgtct ccgataaggt
    21481 tttcaccaga cggtccaaat aagcatctgt tgtttcagtc tctgtcttag gaaaataatg
    21541 ggattttaga gacgattctg ccattgccgc taagtaaccc aaataacggt ctctaacgga
    21601 tgatacttct ccaaaagagc ctgttgccac aaaactaatc tggtcacagc cttgctcaat
    21661 caagtgctga gtcaattgac aggcaccgcc tttattatcc gctacaacag acggaaactg
    21721 aaaaagatct agagatttat ccaacaagac tagaggaatg tgatgatggt cgcagtaaaa
    21781 caggaaatca atactgtgat tgacatcttc aggataataa ataatcccag catagtctga
    21841 cagggtgtct aaccttactg tactggccaa ttgaaccatg agacggtaac ctgtttcagc
    21901 aatggaggtc ataattccct tagcataatc tcccaattca taatcagaag taaaaggaag
    21961 aatcaacaat aaatccttat tcgcttcagg ggacgtcact tgcttttcag ctacgaagct
    22021 tcctttccca cgacttctcg taatcaagcc ttcttgctcc agttctacaa gcgctcgctt
    22081 agaagtaata cggctaaccg agaactgttc tgacaattct ttttcagtag gtaggcggtc
    22141 accaatttgc aaacgacctg atgctatctg ctctttaatg aaatgatata tctttttgta
    22201 taataatttt gtcattcttt actcccgtta tcagtatagc atgtcaaaaa agagttgtaa
    22261 accttttcaa aaacttatag acaaagttta acttatcatt tataatgaaa ccgaagataa
    22321 atgatatatc attttggagg atatgatatg acacgaccaa tcccaactag cgtcagccaa
    22381 tttatggcca aggtggaaag cctttgtgga gaccaacacc ctgattgggc tcttaatttc
    22441 aaaaccagct ttaccaatac cttagagaca accctaaaaa cctacgaaga tggcactagc
    22501 tttctcttga caggagatat tcctgccatg tggttacgtg attccactgc gcaaatgaaa
    22561 ccttacctct tcctcgccaa ggaagatgaa gaaattagaa agattattgc cggtttggtc
    22621 aaacgccaat tccgctacat ttgtatcgat ccctatgcca atgccttcaa cgaagaagct
    22681 aacggaaaag gacaccaaac agatcgtacc caaatgaacc cttggatttg ggaacgcaaa
    22741 tatgagattg attccctctg ctaccctatt cagctagctt acttgctcta tcgtgaaaca
    22801 ggaagcaccg atcagtttaa tgacgatttt catcgtgggg tagaactcat tcttgaccta
    22861 tggacagttg agcaagacca cgcccagtct ccttacttgt ttgaacgcga tacttggcgc
    22921 aaagaagata ccctaacaca tgctggaaaa ggaagtcctg tcgcaccaac agggatgacc
    22981 tggtcaggct ttagaccaag tgatgatgct tgtcaatatg gttacctcat tccttcaaat
    23041 atgtttgcag tggttgtgct ttcctatcta gaagatttat ataacaacct ttttcacaat
    23101 gaaccagtag ctactcgtgc caagcagctg aaagaagcta ttcaatcggg tattgcagac
    23161 catgcccttg ttcaaaacag caagggagaa accatttacg cttatgaagt ggatggtctg
    23221 ggacagtttt ccatcatgga tgatgctaat attccaagcc tccttgctgc accttacctt
    23281 ggtttttgcg ctaaagatga ccctatctat ctggcaacgc gccgcaccat tctcagccaa
    23341 gaaaatcctt attattatca gggaaatgca gcagcaggca tcggttcttc ccatacacca
    23401 gaaaattata tctggcatat cgcccttgcc ttacaaggat tgaccgctct tgaccaagac
    23461 agtaaaaaag agatgcttga tttgcttgtg gcaacagatg ctggtaccca cttaatgcac
    23521 gagggtttcg atgtcaatga tccttatcaa tacactcgtg agtggttctc ttgggctaac
    23581 atgatgtttt gtgaactact tcttgactat cttggcttta gcattactcc ttagaaagtg
    23641 aggccactat ggcaactaaa aaagtacata ttatttcaca cagtcactgg gatcgcgagt
    23701 ggtacatggc ttacgaacaa caccacatgc gtctgattaa cttaatagat gacctgttag
    23761 aagtttttca aacggatcct gattttcata gttttcattt ggatgggcaa accattatcc
    23821 tagatgatta tttgcaagta cgccccgaac gagaacctga gattagacaa gccattgctt
    23881 cgggaaaact ccgtatcgga cctttctata tcttacagga cgattttttg accagcagtg
    23941 aatccaatgt gcgcaatatg ctgattggta aggaagattg tgacagatgg ggcgctagtg
    24001 tgccacttgg ttattttcct gatacctttg gaaatatggg acaaacacca cagctgatgt
    24061 taaaagccgg cctacaagct gctgcctttg gtcgtggcat tcgtccaact ggatttaaca
    24121 atcaagtaga taccagtgaa aaatacagct cacaattctc tgaaatcagt tggcaaggcc
    24181 cagataacag tcgtattctt ggactcctct tcgccaactg gtacagcaat ggcaatgaga
    24241 tcccgacaac agaagctgag gcgcgtcttt tttgggataa aaaacttgct gacgccgaac
    24301 gctttgcctc aaccaagcac cttctgatga tgaacgggtg tgatcatcaa cccgtacaac
    24361 ttgatgtcac caaggcaatc gccttagcca accaactcta tcctgattac gaatttgtgc
    24421 attcctgctt tgaagattac ttgtctgatc tcgcaaatga tttaccagat aacctttcaa
    24481 ccgtccaagg agagattacc agtcaagaga cagatggctg gtatacccta gctaacacgg
    24541 cttctgctcg catttacctc aaacaggcta ataccagagt ctctcgtcaa ctcgaaaaca
    24601 tcaccgaacc cttagcagca atggcttatg aggtgacaag tacctaccct cacgaccaac
    24661 tgcgttacgc ttggaaaacc ctcacgcaaa atcaccctca tgattctatc tgtggttgta
    24721 gtgttgatag tgttcatcgg gaaatgataa accgttttga aaaagcctat gaagtcggac
    24781 actatttagc aaaagaagct gcccagcaaa ttgctggcgc agttaacacg agtgactttc
    24841 caacagatag ccaacctttc ctcttattta ataccagtgg ccattccaaa acaagtgtta
    24901 ctgagctcac cctgacctgg aaaaaatatt attttggcca acgctttcct aaagaggttt
    24961 accaagaagc tcaagagtat ttggcaggac tctcccaatc tttccaagtc attgatgtat
    25021 caggaaacac tatctcagaa gcagacattt tggacacaag catcgccttt gactacgatt
    25081 tgcccaagag aaccttccga gaaccttatt tcgccatcaa agtgagatta cggctaccaa
    25141 taactctccc tgccatgtct tggaaagcct tagcattaca actaggaaac gagacagctc
    25201 cttcagcaac ggtcccactc tacgacgata gtaatcagtg ccttgaaaat gagtttctaa
    25261 aagttatgat acaaaccgat ggccgtctaa ccatcaccga taaacagtca ggggtgagct
    25321 atcaagatct cctacggttt gaagattgtg gcgatattgg aaatgaatac atttcccgcc
    25381 aaccaactca tgaccaacct ttctatgcgg accaagggac caccaagctt aacatcatta
    25441 gcaacaccgc tcaagttgct gaacttgaaa tccagcaaac ctttgccatt cctatctccg
    25501 cagataagct cttacaggct gagatggaag ctgtcattga catcacagaa cgccaagcaa
    25561 gacgttcaca agaaaaggct gagctaacct taacaaccct tatccgtatg gagaaaaaca
    25621 atcctcgcct ccaatttacc acacgttttg ataaccaaat gacaaatcat cgcttgcgcg
    25681 tcctattccc aacgcacctt aaaacagacc atcatctagc tgacagtatt tttgaaactg
    25741 tcaaacgtcc aaatcatcca gatgccacct tttggaagaa tccaagtaac ccacagcacc
    25801 aagaatgctt tgtgagtctc tttgatggtg aaaatggagt cactattggt aactatggcc
    25861 tcaacgaata tgagatctta ccagatacca acaccattgc catcactctc ttacgttctg
    25921 ttggtgaaat gggcgactgg gggtatttcc ctactcctga agcccaatgc ctcggcaaac
    25981 acagcctatc ttatagtttt gaaagcatca ctaagcaaac acaatttgcc agctattggc
    26041 gggcacaaga aggccaagtt cctatcctag tcgaacaaac cagccatcat aagggaagct
    26101 taagagcaga gcatagctat gtcactgggg cagacgatca agtcgctcta acggctttca
    26161 aacgtcgctt agcagacaat gcccttatca cgcgcagcta caatctttca aacgataaaa
    26221 cttgtgactt tagcctaagc ctgccaaact acaatgctaa ggtcactaat ttgttagaaa
    26281 aagacagcaa gcaaagcaca cccagccaac ttggcaaagc tgaaatttta actctagctt
    26341 ggaagaaaca ataaactatt atcattagta atagattaac atattcaaaa agagtctggg
    26401 actattgtct cagactctta agttgatttt gagtgattat taataccttt aacatcagtc
    26461 aggcttagct atttctatta actgggttaa aatgccatca tgaatctgcg agctaattgt
    26521 gagatagtca taacgattta aaatggtcgt gacattcctt aagtcaaatt gccagtcatc
    26581 tctttttttc aaagccggtg tagcaaacac gctttgacta tcattttgtc cttgtttagt
    26641 actgctttga atgactatga caagtttatg gttttgatcc agaaatgcta ttgataagca
    26701 aggttgcttt gtctccaaac tcatttctat agcattatct aacaaaaatg acaatagctt
    26761 agtgaaatct actacttcta gaaaaaacgt tgcaatacaa ttaggaatcg atacctcaac
    26821 cttaagcttc ttattttttg cctcaattaa tttagctaaa acaatatgtc tgattgcttc
    26881 aacttgtaat ttatctaaac aagttaaatc aagtctgtca tctttagcat tattcttttc
    26941 agcattcaag ataccttcat aagtaagtct cacaatagag atatcatttt gttcgattcc
    27001 aatcttaaga cgactggaca cggtagttaa tagctgccta agctcttgaa tatcttgata
    27061 cagcaatcca aggtgctgac tataattaat cagctcacga tattcttttt gttgcacaat
    27121 tgcctcaata gagttttgtt ttacataacg ctctaaatga attaataaag taagaaacaa
    27181 aatgaaataa aagagcacta tgattgaacg agcagtcgtt gtagcaagtc ctgataaaga
    27241 aggcgacatc acatctatcc cttggacaca caagtaatat agagccatcg aagagttagt
    27301 aaacactaat agtttactag ctctatgtga ttgaaaatat tggcgccagg ttgaaaaatc
    27361 gaaatgaaaa acttgaatgc agcgataaat caaaaaaata ctaaagcaag taatgctgta
    27421 aatcagtagt gtattatgtt taacaactga ataaggcata ccaaacaatg gcataacaaa
    27481 aaatacaata aatcgcttaa ccaaactaat agacactagt ggaaaaaagg cataaaacat
    27541 taaaaactta gcttttattg atttaccata gtatagggct ataagcgcaa agaaaagaaa
    27601 ctgagccaac atcacaacat caagaatgaa atggtctgga aagaaagccc aaaaaactac
    27661 ttgaaacaaa accatactaa gtgataaata aaaaatattt ttcaggcgaa gcggaattgc
    27721 acttacaaag ctaaaaagct ttaacgttaa cataatttca ataatatcta aaaatataaa
    27781 tcctaaaaat tgaagaaaat cttctaacat ttatccttct ccttctcgtt caaaatatgt
    27841 tatcacatct cgattttctt ttcatttcga actccgtaat agataaactg agctttttca
    27901 ttttaatcat attctttatt ttatcataag ttctgtcatt ctaacagtta aattacgaaa
    27961 agcttgatta agtaactttt ctatattccg atcattttac tggttatcaa ctcagattta
    28021 tcagttgttg atctcattat aaacaagtgt tgcatttatc tttaatatct aacacataaa
    28081 gactcacaca gcctacagtt tttgcaaagg tttcttacag cttaaaagat taaatcagga
    28141 taaatcctct atatcatcgt tcatattagc atactaaaat aacttctggt aatgtaacct
    28201 cctgaccact cagttttcta atttatagca aaaaacagct ttttttcaac aaccaattcc
    28261 cctaatattg tctgtgtgta cttgctcttc aaataaaccc tacgtagtga caaactaaca
    28321 aactttttta accatgaatg tttctaaaat aaacatagag ttatcaataa tatgactatg
    28381 cttagtaaac cgtttttgac atccctacta tgtcaagcta ttgctcaaac ccattacctg
    28441 agcttataaa acttgagttg ttattgccac tcaatcatac acgtttttgt aacttcacga
    28501 cagcctcggt cctagctgta tgggggaaca tgtctacact ttggatatag tgaacatcgt
    28561 aaacctttgt tagctgcacc aagtcgcggg ccaaagtgga ggtattacaa gacacataca
    28621 ccatctgttt aggctggtaa tgaagaatcg tttttaataa tttatcatct aaaccagttc
    28681 ttggtgggtc cacaatgatg gcatccgcgc ggtaaccttg tttataccat ttaggaataa
    28741 tatcctctgc cttccctgcc tcatagtaag cgttgtcaaa gcccatggct ttggcatttt
    28801 tttgtgcatc ttcaatcgct tctgggataa tatccatccc ccgcacagac ttgaccttcc
    28861 ctgcaaaagc aaagccaatc gagccaaccc cacaataggc atcaataata tgatctttgc
    28921 tccctacatc caaggccttc accacctcac catacaaaac ttctgtctgc tgtggattaa
    28981 gttggtaaaa agctctcggt gataacgcaa atccatagtc taaaacttct tcatgaatcg
    29041 cctcttgtcc ccatagaatt tccgtttcgt ccccataaat ctcactcgat ttagaacgat
    29101 tactattcaa ggcaaccgtt ttaacctgag gaaatgcctt agtcaattcg ccaatgaaat
    29161 tcgctaaccg aacttctttg ctactcacaa caataatctg tacctgatca ctagcctgtg
    29221 ccttacgaac cattatcgtc cgaatcccag caatcttgcg ctcattgtaa atgggcaact
    29281 tgtacttgtc cactaactgc gtaatcttat tgataatatc ttgcgttagt tgatcctgta
    29341 ccaagcaatt gtcaatagga accaagcgat gacttccctg ggaaaataaa ccagccttga
    29401 ccgtcccgcc aaatgaccgc agctgaaact gcaatttggc tcgatagtga tctggctttt
    29461 tcattcctaa agtcggtcta atctcaaatt gttcatagcc agttggtttg aactttttaa
    29521 gggcctgcct aatcacatca tctttgaaat ctagctgttt aggataggct agatgcatga
    29581 tttggcaacc accacaagtt tcataaacag aacaagctgg tttgacacga ttcttagaag
    29641 ccttgtttac catcaacagt ttggcctctg caaaattccg tttaaccgac gtgatttggc
    29701 aaaaaatatc ttctcccttt aaagcaccag ggacaaatac caaagttttc tgataaaaac
    29761 caatcccctc accgttaatt cccattcgct taatttttaa aggaattttc tgtttaacct
    29821 ttacgaccat ctcttcccct tacttcctct aatatcctaa tctcaccacc aaaagcattc
    29881 acaagcaaca catttttagc agtattttcg tcttctattt taccattttt gatataatga
    29941 acatatgaaa atcactaaaa ttgaaaagaa aaaacgcctc taccttatcg aattggataa
    30001 tgacgattcc ctttatgtaa cagaagatac tattgttcgg tttatgttga gtaaagataa
    30061 agtccttgac aatgatcagc ttgaagacat gaaacatttt gcccaactgt cctacggcaa
    30121 aaatttagcc ctttattttc tttcctttca acaacgcagc aacaagcaag ttgctgatta
    30181 cctgcgcaag catgagattg aagaacacat tattgctgac atcatcactc aactccaaga
    30241 agaacaatgg atagacgaca ccaaattggc tgatacctac attcgccaaa atcagttaaa
    30301 tggtgataaa ggtccccaag tcttaaaaca aaagttatta caaaaaggca ttgcaagtca
    30361 tgacattgat cctatcttat ctcaaactga ctttagccaa ctcgctcaaa aagtaagcca
    30421 aaaactcttt gacaaatatc aagaaaaatt gccaccaaaa gccttgaaag ataaaatcac
    30481 ccaagcatta ctgaccaaag gcttttcata cgatctagct aaacatagcc tcaatcacct
    30541 taattttgac caagataatc aagaaataga agatcttctt gacaaagaat tagacaaaca
    30601 atatcgtaaa ctcagtcgca aatatgatgg ttatacttta aagcaaaagc tctatcaggc
    30661 tctctaccga aaaggctaca acagcgacga tattaattgc aagttaagaa attatttata
    30721 gactgacaaa agaagtgatt ttttcacaca tttatgatag actatagagg ataagtttaa
    30781 ttgtagaaag ttggtaaggc atgaaattac ctaaagaagg cgactttatt acaattcaaa
    30841 gttataagca tgatggtagt ttgcaccgta cttggcgcga tactatggta ctaaaaacaa
    30901 ctgaaaatgc cctcattgga gttaatgatc acactcttgt cacagaaagt gacggtagac
    30961 gatgggtgac ccgcgaacct gcaatcgttt actttcataa aaaatactgg tttaacatta
    31021 tagccatgat tcgagataat ggcgtctctt attactgtaa cttagctagt ccatacatga
    31081 tggacactga ggccctcaaa tacattgatt acgatttaga tgtcaaagtc tttgcggatg
    31141 gtgaaaagag attacttgat gttgatgaat acgagattca taaaaaagag atgcagtatt
    31201 cagccgatat ggactttatc ctaaaagaaa atgtcaaaat attggttgat tggattaatc
    31261 atgaaaaagg accattttcc aaggcctata tcactatttg gtacaaacgt tatcttgaac
    31321 tgaagaatcg ttaagagttg tccagcactc ccaaggagtg ctcagaacgc agacaaactc
    31381 agttttaggc tcaaaaagca tgaacagctc tgctgatttg acatccaatt tcctatttct
    31441 gcgtgtggag tgagccaaaa cagatgtttt tcgttgtggt aaaagtggcg tcaatgttaa
    31501 tgttgacgcc acttggcatt tttgagccta ggatctcaaa aataaggcat ggaattccaa
    31561 agaaagtcgc tgccttaagc actacctaag gaaagtatca aaaaatacta agtttagtag
    31621 cgaggaaatc tccgacgaaa gagagtactc actacttttt ctttgcgtta aagtagaagt
    31681 gtcttgttaa gtcgcaaagt cttttggcac ttgatgttgt aaccaaaact gacaagacac
    31741 ctcttttagg tagaatttta tcaaagtatg taggacgctt gacgtcgtgt attgaaaatg
    31801 aaaccactaa aataaggaat cattcaagac aaaacgatac catcggcctc aatcatcttc
    31861 ttggaaactt gaaaaccgtc cataaccctg agattatcct taaaacgatt aatgtctatt
    31921 ctcgtcgtct tcaggttttt tcgcagataa tggctacgct tatactcggt ttaatccttt
    31981 agaagctaag aagcaattga atagcctgca cgttcgtaaa acagacaaaa ttgacactga
    32041 gaagaagtct aataaaatct gacagaagat attgtgggag ttaagaatcg tctgcataag
    32101 cttttccaag tcaccttttg tcttactata actgctaaaa ttaccattaa aaccacatta
    32161 agatctccat accatccatt actatccatt aattaaacaa tttcttctat aatgggattt
    32221 gtaatatgtg catacaacct acaacaacac atgacttgag aacattgtaa agtgtctttt
    32281 aaacctcaat ctgcaattaa aaagaaacaa taaaaagctt aaatttgtta tacaacaaat
    32341 ttaagctttt tatcattaaa atacaatcaa ccaagttagt tcgattactt taaccaaagt
    32401 tactaaatag
//
LOCUS       DATXIG010000016        28122 bp    DNA     linear   BCT 28-DEC-2023
DEFINITION  TPA_asm: Streptococcus pyogenes strain MGAS11113
            SAMN08555799-rid19682583.denovo.016, whole genome shotgun sequence.
ACCESSION   DATXIG010000016 DATXIG010000000
VERSION     DATXIG010000016.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN08555799
            Sequence Read Archive: SRR6740495
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pyogenes
  ORGANISM  Streptococcus pyogenes
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 28122)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 28122)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-OCT-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-OCT-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001918058.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001918058.2
            Genome Coverage       :: 368x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/12/2023 11:23:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 1,806
            CDSs (total)                      :: 1,769
            Genes (coding)                    :: 1,722
            CDSs (with protein)               :: 1,722
            Genes (RNA)                       :: 37
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1, 1 (5S, 23S)
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 47
            CDSs (without protein)            :: 47
            Pseudo Genes (ambiguous residues) :: 0 of 47
            Pseudo Genes (frameshifted)       :: 30 of 47
            Pseudo Genes (incomplete)         :: 20 of 47
            Pseudo Genes (internal stop)      :: 9 of 47
            Pseudo Genes (multiple problems)  :: 11 of 47
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..28122
                     /organism="Streptococcus pyogenes"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN08555799-rid19682583.denovo.016"
                     /strain="MGAS11113"
                     /serotype="Emm28"
                     /isolation_source="normally sterile site"
                     /host="Homo sapiens"
                     /db_xref="taxon:1314"
                     /country="Finland"
                     /collection_date="2002"
                     /collected_by="National Public Health Laboratory"
     gene            <1..191
                     /locus_tag="VC122_000761"
     CDS             <1..191
                     /locus_tag="VC122_000761"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011284459.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEP5640703.1"
                     /translation="MIATAAVAGLSLATPENQTVSANETTTTQACADGTNCCGYNFRG
                     YCGPITWLYYCIRDGKIY"
     gene            complement(507..893)
                     /locus_tag="VC122_000762"
     CDS             complement(507..893)
                     /locus_tag="VC122_000762"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012767609.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEP5640704.1"
                     /translation="MKQQSYQPLRLVYLLVALFAAMLLIARPVMADGEQKDPWELGYN
                     QGLTKGKEDGESAKWTDLTPTVPTDPTIPQPPTVIEETNKGIYKEGYKDGYKEGYNKG
                     FGGSYPLLIPVQFIWGLILHWWGKLS"
     gene            complement(1148..2863)
                     /gene="slo"
                     /locus_tag="VC122_000763"
     CDS             complement(1148..2863)
                     /gene="slo"
                     /locus_tag="VC122_000763"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_095889392.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cholesterol-dependent cytolysin streptolysin O"
                     /protein_id="HEP5640705.1"
                     /translation="MSNKKTFKKYSRVAGLLTAALIIGNLVTANAESNKQNTASTETT
                     TTNEQPKPESSELTIEKAGQKMDDMLNSNDMIKLAPKEMPLESAEKEEKKSEDKKKSE
                     EDHTEEINDKIYSLNYNELEVLAKNGETIENFVPKEGVKKADKFIVIERKKKNINTTP
                     VDISIIDSVTDMTYPAALQLADKGFTENKPDAVVTKRNPQKIHIDLPGMGDKATVEVN
                     DPTYANVSTAIDNLVNQWHDNYSGGNTLPARTQYTESMVYSKSQIEAALNVNSKILDG
                     TLGIDFKSISKGEKKVMIAAYKQIFYTVSANLPNNPADVFDKSVTFKELQRKGVSNEA
                     PPLFVSNVAYGRTVFVKLETSSKSNDVEAAFSAALKGTDVKTNGKYSDILENSSFTAV
                     VLGGDAAEHNKVVTKDFDVIRNVIKDNATFSRKNPAYPISYTSVFLKNNKIAGVNNRT
                     EYVETTSTEYTSGKINLSHRGAYVAQYEILWDEINYDDKGKEVITKRRWDNNWYSKTS
                     PFSTVIPLGANSRNIRIMARECTGLAWEWWRKVIDERDVKLSKEINVNISGSTLSPYG
                     SITYK"
     gene            complement(2887..3372)
                     /locus_tag="VC122_000764"
     CDS             complement(2887..3372)
                     /locus_tag="VC122_000764"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003062498.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD glycohydrolase inhibitor"
                     /protein_id="HEP5640706.1"
                     /translation="MYKVPKGLEHYQKMFQKEVTVNDLKKYLIGSDKEYRITRRDSYM
                     GDISDPEVILENGVYPAFLKGYTQLKANIEEALLEMSNSGQALDIYQAVQTLNAENML
                     LNYYESLPFYLNRQSILANITKALKDAHIREAMAHYKLGEFAHYQDTMLDMVERTIET
                     F"
     gene            complement(3377..4732)
                     /gene="nga"
                     /locus_tag="VC122_000765"
     CDS             complement(3377..4732)
                     /gene="nga"
                     /locus_tag="VC122_000765"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002986398.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nicotine adenine dinucleotide glycohydrolase"
                     /protein_id="HEP5640707.1"
                     /translation="MRNKKVTLAHIVAKTSVAIALAGAMGSSLLANSTTYAVSGKENK
                     KSDVKYETTKVMEANATSSKEDNHVMHTLDGSMSTVWEENSPGGGVGEVLSYKFASPM
                     RIGRILIVNGDTSSKENYYKKNRIAKADVKYYNGNKLVLFQKIELGDTYTKKPHHIEI
                     DKKLDVDRIDIEVTEVHQGQNKDILALSEVTFGNMERDLFEKKFKEIKDKWVTDKQAD
                     EFIETADKYADKAVQMSAVASRAEYYRMYVSRKYHYKKEFVEKLKQVYKESGASHVTS
                     KKDLMLAFDDAKRKSTIGRQENGLFVTSFAEDMALLFTDQGKLKSADQIENIKGVDSG
                     RYSDGVYQYEYDSELTKNIDKLGYIRTASGDTPGANSLNIPGCQTWSGKHIENSESEL
                     IFPSISVKDLKSKAVLAEIDAKGYFEIIDPTIIAPNGDHKKVTGRFKIKKMQDRMQDR
                     K"
     gene            complement(5103..5642)
                     /gene="nusG"
                     /locus_tag="VC122_000766"
     CDS             complement(5103..5642)
                     /gene="nusG"
                     /locus_tag="VC122_000766"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003100341.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription termination/antitermination
                     protein NusG"
                     /protein_id="HEP5640708.1"
                     /translation="MLDSFDKGWFVLQTYSGYENKVKENLLQRAQTYNMLDNILRVEI
                     PTQTVNVEKNGQTKEIEENRFPGYVLVEMVMTDEAWFVVRNTPNVTGFVGSHGNRSKP
                     TPLLEEEIRAILLSMGQTIDVFDTNIKEGDVVQIIDGAFMGQEGRVVEIENNKVKLML
                     NMFGSETVAEVELYQIAEL"
     gene            complement(5815..6858)
                     /locus_tag="VC122_000767"
     CDS             complement(5815..6858)
                     /locus_tag="VC122_000767"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011888557.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BMP family protein"
                     /protein_id="HEP5640709.1"
                     /translation="MNKKVMSLGLVSTALFTLGGCTNNSAKQITDNSLKIAMITNQTG
                     IDDKSFNQSAWEGLQAWGKENKLEKGKGYDYFQSANESEFTTNLESAVTNGYNLVFGI
                     GFPLHDAVEKVAANNPDNHFAIVDDVIKGQKNVASITFSDHEAAYLAGVAAAKTTKTK
                     QVGFVGGMEGDVVKRFEKGFEAGVKSVDDTIKVRVAYAGSFADAAKGKTIAAAQYAEG
                     ADVIYHAAGGTGAGVFSEAKSINEKRKEEDKVWVIGVDRDQSEDGKYTTKDGKSANFV
                     LTSSIKEVGKALVKVAVKTSEGQFPGGQITTFGLKEGGVSLTTDALTQDTKKAIEAAK
                     KAIIEGTITVPEN"
     gene            complement(7185..8477)
                     /locus_tag="VC122_000768"
     CDS             complement(7185..8477)
                     /locus_tag="VC122_000768"
                     /EC_number="6.3.4.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000205037.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenylosuccinate synthase"
                     /protein_id="HEP5640710.1"
                     /translation="MTSVVVVGTQWGDEGKGKITDFLSADAEVIARYQGGDNAGHTIV
                     IDGKKFKLHLIPSGIFFPQKISVIGNGVVVNPKSLVKELAYLHDEGVTTDNLRISDRA
                     HVILPYHIQLDQLQEDAKGDNKIGTTIKGIGPAYMDKAARVGIRIADLLDKDIFAERL
                     RINLAEKNRLFEKMYDSTPLDFDAIFEEYYAYGQEIKQYVTDTSVILNDALDAGKRVL
                     FEGAQGVMLDIDQGTYPFVTSSNPVAGGVTIGSGVGPSKINKVVGVCKAYTSRVGDGP
                     FPTELFDEVGERIREVGHEYGTTTGRPRRVGWFDSVVMRHSRRVSGITNLSLNSIDVL
                     SGLDTVKICVAYDLDGKRIDYYPASLEQLKRCKPIYEELPGWQEDITGVRSLDELPEN
                     ARNYVRRVGELVGVRISTFSVGPGREQTNILESVWASI"
     gene            8915..9793
                     /locus_tag="VC122_000769"
     CDS             8915..9793
                     /locus_tag="VC122_000769"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011054137.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEP5640711.1"
                     /translation="MIRKYDRTSTKNKSLNWIWLIIAFFMISSFIGGSSFTESLLDIL
                     PAIAIGGTGYAIFRVRSHQKRLAKAKIAKQLEDLKAKIQLADRKVRLLDTYLADHDDF
                     QYNVLAQQLLPQLSDIKAKAITLKDQLDPKIYRRITKKANDVESDITLQLETLQIATT
                     LNPQPLKTPSPNLINKAPELKPYYDNIQTDHQAILAKIQGADNQEELLALHEANMRRF
                     EDILTGYLKIKEEPKNYYNAAARLEQAKQAIQQFDEDLDETLRRLNESDLKDFDISLR
                     IMQGATQRRTTHHQKD"
     gene            9806..11068
                     /locus_tag="VC122_000770"
     CDS             9806..11068
                     /locus_tag="VC122_000770"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011017278.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="toxic anion resistance protein"
                     /protein_id="HEP5640712.1"
                     /translation="MADFNFDIDQIADNAVVKTDKTTDIISDLPTDTNGQISFFEKLS
                     ADQQTAITAKAPALVDTFLADQNALLDFGQSAVEGVNATVNHILAEQKKLQIPQVDDL
                     LKSTNRELNGFIAKYKDATPVDLDKKPNFLQKLFKQSRDTLQEFYFDSQNIEQKMDNM
                     AAAVVKQEDTLARNIVSAELLIEDNTKSIEHLVGVIAFIEASQKEASQRAAALQKDLK
                     TKDSATPDYQIKADLLARTTEVINTLEQQHTEYLSRLYVAWATTPQMRNLVKVSSDMR
                     QKLGMLRRNTIPTMKLSIAQLGMMQQSVKSGMTADAIINANNAALQMLAETSKEAIPA
                     LEQSAQNPTLSIKSVTSLAESLVAQNNGIIAAIDHGRKERAQLESAIIRSAETINDSV
                     KLRDQNIVQALLSEGKETQKTIDKTTRA"
     gene            complement(11188..11814)
                     /locus_tag="VC122_000771"
     CDS             complement(11188..11814)
                     /locus_tag="VC122_000771"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003046251.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="V-type ATP synthase subunit D"
                     /protein_id="HEP5640713.1"
                     /translation="MARLNVKPTRMELSNLKNRLKTATRGHKLLKDKRDELMRRFVDL
                     IRENNELRQTIEKELAANMKEFVLAKASENSLMVEELFAVPVHEVTLWIDIENIMSVN
                     VPKFHVQSNTAREQEQGEFAYSYLSSNSEMDNTIQKTKELLEKLLRLAEVEKTCQLMA
                     DDIEKTRRRVNGLEYSIIPQLEETIHYIELKLEEAERASLVRIMKITS"
     gene            complement(11859..13274)
                     /locus_tag="VC122_000772"
     CDS             complement(11859..13274)
                     /locus_tag="VC122_000772"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002986419.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="V-type ATP synthase subunit B"
                     /protein_id="HEP5640714.1"
                     /translation="MSVLKEYRTVSEVVGPLMIVDQVAGVHYNELVDITLHNGERRKG
                     QVLEVQGDKAMVQLFEGSTGINLAKTKVRFTGHPLELAVSEDMVGRIFDGMGHPIDGG
                     PELIPEKYLDIDGQAINPVARDYPDEFIQTGISAIDHLNTLVRGQKLPVFSGSGLPHN
                     ELAAQIARQATVLNSDDNFAVVFAAMGITFEEAEFFMNDLRETGAIDRSVLFINLAND
                     PAIERIATPRIALTTAEYLAYEKGMHVLVIMTDMTNYCEALREVSAARREVPGRRGYP
                     GYLYTNLSTLYERAGRLIGKKGSVTQIPILTMPEDDITHPIPDLTGYITEGQIILSQE
                     LYKNGFRPPINVLPSLSRLKDKGSGEGKTRQDHAATMNQLFAAYAQGKQAKELAVVLG
                     ESALSETDKLYVAFTNRFEEEYINQGLYTNRSIEESLDLGWELLSILPRTELKRIKDD
                     MLDRYLPKADTTMTKVFVAND"
     gene            complement(13275..15050)
                     /locus_tag="VC122_000773"
     CDS             complement(13275..15050)
                     /locus_tag="VC122_000773"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011017275.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="V-type ATP synthase subunit A"
                     /protein_id="HEP5640715.1"
                     /translation="MNQGKIITVSGPLVVASGMQEANIQDICRVGHLGLVGEIIEMRR
                     DQASIQVYEETSGIGPGEPVVTTGCPLSVELGPGLISEMFDGIQRPLDRFQKATDSDF
                     LIRGVAIPSLDRKAKWAFIPKLSVGQEVVAGDILGTVQETAVIEHRIMVPYKVSGTLV
                     AIHAGDFTVTDTVYEIKKEDGSIYQGSLMQTWPVRQSRPVAQKLIPVEPLVTGQRVID
                     TFFPVTKGGAAAVPGPFGAGKTVVQHQIAKFANVDIVIYVGCGERGNEMTDVLNEFPE
                     LIDPNTGQSIMERTVLIANTSNMPVAAREASIYTGITIAEYFRDMGYSVAIMADSTSR
                     WAEALREMSGRLQEMPGDEGYPAYLGSRIAEYYERAGRVRTLGSQEREGTITAIGAVS
                     PPGGDISEPVTQNTLRIVKVFWGLDAPLAQRRHFPAINWLTSYSLYQDDVGSYIDRKQ
                     QSNWSNKVTRAMAILQREASLEEIVRLVGLDSLSEQDRLTMAVARQIREDYLQQNAFD
                     SVDTFTSFPKQEAMLTNILTFNEEASKALSLGAYFNEIMEGTAQVRDRIARSKFIPEE
                     NLEQIKGLTQKVTKEIHHVLAKGGI"
     gene            complement(15251..15571)
                     /locus_tag="VC122_000774"
     CDS             complement(15251..15571)
                     /locus_tag="VC122_000774"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002986425.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="V-type ATP synthase subunit F"
                     /protein_id="HEP5640716.1"
                     /translation="MTKTLYKVGVIGNRDVILPFQMIGFQTFPVIKPQDAINQLRQLA
                     MEDFGIIYITEDIAAAIPEALTHYDNQVLPAVIPLPTHQGAQGIGLSRIQAMVEKAVG
                     QNIL"
     gene            complement(15568..16566)
                     /locus_tag="VC122_000775"
     CDS             complement(15568..16566)
                     /locus_tag="VC122_000775"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011528182.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="V-type ATPase subunit"
                     /protein_id="HEP5640717.1"
                     /translation="MPTFLELNTTISVKEKELLTKEQFDKLLQAPNTTTLARLLHQSV
                     YHLTVDDLNDLDRLESILMAELTKTYRWAFAETPQPDIVQLFTLRYTYHNVKVLLKAK
                     ASQADLSHLLLPIGDKPLVALEHLIRTMTSDEFPKEVVTETQSIWAEYQDYQDIRVLE
                     IGTDLAYFKALKQIAQRLEDPVFQQAVLIVIDLYNLITVRRAKSQNKPISFMMQLLSD
                     EASRPSKTFITLEDDKDLMTWFENVTPDSYMTALKPYSEKLRQGTLQTTELEYLVDEC
                     LYHLFAKAKYQVDGPYVLARFLLAKSFEVKNLRLLAAALANDLPKERVIERMRPIA"
     gene            complement(16582..17166)
                     /locus_tag="VC122_000776"
     CDS             complement(16582..17166)
                     /locus_tag="VC122_000776"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002986431.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATPase V"
                     /protein_id="HEP5640718.1"
                     /translation="MNDITQLRQNVLEKAHQEGQQCLKIATDSLDTDFKERQQQGLHD
                     LKAKRQKELKALEQQFQVAQQQLKNQERQALLALKQDSIKELFEASLEKMTNFSKEEE
                     LAFLKQVLSKYPEQPLQVTFGEKTGQKFSSYDCAELRLAFPQLSYNQELIPQEAGFLV
                     SLDQVDDNYLYRYLLESVLKEESSRIIDMLFSEI"
     gene            complement(17234..17713)
                     /locus_tag="VC122_000777"
     CDS             complement(17234..17713)
                     /locus_tag="VC122_000777"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002993834.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="V-type ATP synthase subunit K"
                     /protein_id="HEP5640719.1"
                     /translation="MEHLAHYFTAHGGVFFAALGIVLAVALSGMGSAYGVGKGGQAAA
                     ALLKEEPEKFTSALILQLLPGSQGIYGFAIGILIWMKLTPELSVNQGLAYFLVSLPIA
                     IVGYFSAKHQGNVSVAGMQILAKRPKDFMKGVILAAMVETYAILAFVVSFILLNKIG"
     gene            complement(17715..19715)
                     /locus_tag="VC122_000778"
     CDS             complement(17715..19715)
                     /locus_tag="VC122_000778"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011888551.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="V-type ATP synthase subunit I"
                     /protein_id="HEP5640720.1"
                     /translation="MAISQMKKLAMVFEKDYLDLVLKTLQQSQLVEVRDMKQLKHWQD
                     AFNKGNVKLPQIVQYDLTHQKPLLDDEALQYLLQSQQELENGLASLSAFLPPIGKLTA
                     LRQKTPSLSFKQFEERHRQQAAQTALKMMSQKIERLEQLQNKIDQLTEYCQELEKWRS
                     LTVLPQDLAQFHFLSARVGTIPSTANDHFYHQLKKHKGLFIEEVYHTEFEYGLVLFWQ
                     AQDTIHLQKYQFKPLLYKEQLLPSEQLRINKELLTNWLAEKDSLLKELRQSQKILAQL
                     QVEIDYVLSQYQRQQTKKQLLGTRHLIALEGWIEADSVNQLKGLMTKTLGDMFYLDSY
                     DVTPDDWEDVPIKLRNHRYIAPFELVTEMYALPKYQEKDPTPFLAPLYLTFFGMMVAD
                     LGYGLLLYAVTLAALVFFNLQKTSKRLVTFFNILAISVALWGLIYGSFFGFDLPVALL
                     STKTDVITILVVSLLFGFVTLIFGLLLGAWQQVRMKAYATAYTSSLAWTFILLGLLLF
                     ILGKNVSGLAYLSVIGKWLALGNAFGILVVSLLKSKSLLGLGSGLYNLYGISSYLSDL
                     VSFTRLMALGLSGASIGAAFNMIVGIFPPVTRFTVGIFIFILLHAINIFLSMLSGYVH
                     GARLIFVEFFGKFYEGGGKAFNPLKLADNYVNVNEETDLEDN"
     gene            complement(19705..20025)
                     /locus_tag="VC122_000779"
     CDS             complement(19705..20025)
                     /locus_tag="VC122_000779"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014407243.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEP5640721.1"
                     /translation="MNTLLSLMNSIEQEARTIYDSYDAKKKALADKKEEELKQLFLEY
                     EQETQLLLEAKREVFFEANRQEQKASKKRLATQQLALHAILEEHKLTVVRELVDKVVE
                     TYGH"
     gene            complement(20479..21498)
                     /locus_tag="VC122_000780"
     CDS             complement(20479..21498)
                     /locus_tag="VC122_000780"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001021299.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS fructose transporter subunit IIC"
                     /protein_id="HEP5640722.1"
                     /translation="MDIIIGTSLLILVLAIFSLFNYKAPHGAKAMGALASAACASFLV
                     EAFQDSFFGKVLGFQFLSEVGGANGSLSGVAAAILVAIAIGVSPGYAVLIGLSVSGTG
                     IIPGFVAGYVVSFLIKWMEKNIPGGLDLISIIIVGAPLTRFLAQLITPVINSTLLTIG
                     DILTSSANSNPIIMGMILGGTIVVVATAPLSSMALTAMLGLTGIPMAIGALSVFGSSF
                     MNGVLFYRLKLGERKDNIAFAIEPLTQADVTSANPIPIYVTNFVGGAACGVLIALMKL
                     VNDTPGTATPIAGFAVMFAYNPVAKVLITALGCIIISLIVGYIGGSVFKNYRLVTKQE
                     LQARN"
     gene            complement(21535..21900)
                     /locus_tag="VC122_000781"
     CDS             complement(21535..21900)
                     /locus_tag="VC122_000781"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014407242.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RidA family protein"
                     /protein_id="HEP5640723.1"
                     /translation="MKSYPEPMGPYSTYTIEGHFLYTAGQLPLNPVTGQLSDGFEAQC
                     RQVFVNLQSILAEQKLDLNHIYKLNVYLTDVTNVEILNHVMTDLFEEPYPVRTAVQVS
                     ALPLQALIEVEAVARVKQL"
     gene            22074..22754
                     /locus_tag="VC122_000782"
     CDS             22074..22754
                     /locus_tag="VC122_000782"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014407241.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator"
                     /protein_id="HEP5640724.1"
                     /translation="MDKETLNYWKTVITFLHDVLGDNYEIILHVIDKNDIYIGELVNS
                     HISGRSKQSPLTTFALDLITNKVYKEKDFVTNYKAIVSPQHKEVRGSTFFIKDKKGNL
                     EGMLCINLDISAYQGVARDLLKLVNLNLEHFIPTAKEPKTVTPQPEEAVEILTSNIQD
                     IIGQIIDPSLLRHNVHLSQDVKIDIVAKLYEKGVFQLKGAVSKVADILCISEPSVYRY
                     LKKIEADQ"
     gene            complement(22875..23522)
                     /locus_tag="VC122_000783"
     CDS             complement(22875..23522)
                     /locus_tag="VC122_000783"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011054131.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-oxoacid CoA-transferase subunit B"
                     /protein_id="HEP5640725.1"
                     /translation="MAVALSKEEIQNRIAKRVAKELEDGTLVNLGIGLPTKVANFVPE
                     EMTVYFQSENGFIGLGPKSDDPNSTIVNAGGQPVTVYPGAAFFNSADSFGIIRGGHVD
                     LTVLGALEIAENGDIANYLIPGKMVPGMGGAMDLLVGAKKVIVAMEHTNKGKHKLLKE
                     CTLPLTAKGVVDLIITEMGVFKVTPDGIQVIEISEGFTFDEVQAATGVPLSIAEG"
     gene            complement(23525..24184)
                     /locus_tag="VC122_000784"
     CDS             complement(23525..24184)
                     /locus_tag="VC122_000784"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002986455.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CoA transferase subunit A"
                     /protein_id="HEP5640726.1"
                     /translation="MKENKRIAIAEAISHIKDGDTIMVGGFMANGTPEALIDALVDKG
                     TKDLTLICNDAGFVDRGVGKMVANHQFKTIYATHIGLNKEAGRQMTAGETTIELIPQG
                     TFAEKIRIGAYGIGGFYTPTGVGTLVAEGKETKTIKGKTYLLEYPFEADVTLIFANKA
                     DEMGNLQYSGSENNFNQLMAACAKTTIVQVREIVPVGTIQPECVHTPHIFVDYIVKEG
                     V"
     gene            complement(24196..25380)
                     /locus_tag="VC122_000785"
     CDS             complement(24196..25380)
                     /locus_tag="VC122_000785"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002986460.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA C-acetyltransferase"
                     /protein_id="HEP5640727.1"
                     /translation="MTKEVVITSAYRTPIGNFGGVFKSLSAVDLGVTVVTKILADTGL
                     KSDAIDEVIFGNVLHAGLGQNVARQVALNAGLSYDTPAFTIDMVCGSGLKAVELGAQK
                     IQTGNADIVLVGGTENMSQAPYVLQGQRWGSRMGDSKVVDTMLKDGLSDAFAGYHMGI
                     TAENIVQQYGLTREEQDAFAADSQRKAQLAIEKGRFKEEIAPVTIPQRKGEPLLVDQD
                     EYPKFGTTVDKLAKLRPAFIKDEGTVTAGNASGINDGAAAILLMSKEKAEELGLPILA
                     KITSYASAGVDPSIMGCGPIPATKKALAKAQLTIDDIDLIEANETFAAQALAVSRDLG
                     FDNEKVNVNGGAIALGHPIGASGARILVTLLAEMAKRDVRHGLATLCIGGGQGQSIIV
                     TR"
     gene            25501..26412
                     /locus_tag="VC122_000786"
     CDS             25501..26412
                     /locus_tag="VC122_000786"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002993827.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HEP5640728.1"
                     /translation="MNISSLAYFIEIVNCDFNLTKAAQKSHISQPALSSYIKTIEKEE
                     GVVLFQRQKGRLVGLTSVGERFFENAKVVIASHEQLMADLRSESKKYKGEISIGIPQL
                     ISGIVFSELIPKLIEENPEAKFHIVETGAYELQKKLFLKEVDFAILLTPTTLDATIAD
                     IDILLRDQLCLYMSHKTFQETFDSNPNISIKALHRQNLVTFDKTFMINHQLTTLFEEA
                     NSLPVVKCYSASWDFLLNCTRYSSYLTILPRPITHFAHMDGLVEVQLTEHPKWEVVLA
                     SLKHNKTSHLKHYIKHTILDYFMNQRF"
     gene            complement(26482..27888)
                     /locus_tag="VC122_000787"
     CDS             complement(26482..27888)
                     /locus_tag="VC122_000787"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002987851.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="short-chain fatty acid transporter"
                     /protein_id="HEP5640729.1"
                     /translation="MNNIKTKKRFMDRYIDGFMKWMPESLFICFILTFLVVTMSVLMT
                     DSPFIGTEKTGGIIYGWVNGFWGLLSFAMQMTILLATGNAVASSPPAHKMFKSLAKLP
                     QTRTQIFIFSIVVGSIFGFLHWGLGMMVAIVFGKELLVQARQKGIKVHTPLFVATLFF
                     TFLPATSGLSGAAVLYSATPDYLRNSVADAYKQVVPESVPLTESVLNLPFISLLVVCM
                     LVPLCFALLAHPKDETKIMELDDEIYHHSLDTASHVVIARNTPAEKMNASRLVMYLVG
                     GAIVSYSLYHFSVVGLSGLDLNSFNFLFLGLGLLLCGQQGPEYYGSLFKDGVMSSWGL
                     VLQFPFYAGIFGIIQSTGLGLEISHFFVAISNGTTWPVFAYLYSALLNIAVPSGGSKF
                     VIEAPYIVPATIEVGNDLGKILQAYQLGDATTNLIVPFWALSYLSNFKLKFNQIVAYT
                     IPCVLVVTGIAIIYLFVF"
BASE COUNT         8517 a         6025 c         4909 g         8671 t
ORIGIN      
        1 ttatgatcgc aactgctgct gttgcaggcc ttagccttgc aacaccagaa aaccaaactg
       61 tttcagcaaa tgagactaca actactcaag catgtgcaga tggaacaaat tgctgtggtt
      121 acaatttcag agggtattgc ggccctatca cttggcttta ctattgtatc cgagacggaa
      181 aaatttacta atattacatc taaattaaga gaaatatctc attagtaagt ctgaagtact
      241 caaaagctta ggtgcctatt taggcagtcc tgggcttttt tgctgtccta aagttagggt
      301 gttgccacaa cttcaaaccc aaagacaatt aaaccgcacc ccaacaatta gacataggct
      361 atgtccccag aatcctgggc acatatcact aaaacctaat gattaaagtg cggtttaagg
      421 tgtggtagca aggaactgtt ttaaattcgt tttttcctaa ctatagccac tagctatgga
      481 atcagcttag agttgattat caaatgtcag ctcaacttcc cccaccaatg taaaatgagt
      541 ccccatataa actggacagg aattaacaaa gggtagcttc ctccaaatcc tttattataa
      601 ccctctttgt acccatcttt atatccttct ttataaatac ctttattagt ctcttcaata
      661 acagtaggtg gttgtggaat agttggatca gttgggacag ttggggtcaa atcagtccac
      721 tttgccgatt caccatcttc tttccccttt gttaaacctt ggttatagcc tagttcccac
      781 ggatcttttt gttccccatc cgccataaca ggtcttgcta taagcaacat agcagcaaat
      841 aaagccacca agaggtagac gaggcgtaga ggctggtaag attgttgttt catgataata
      901 gttatctctc ccttttatat tatgctttgc ttgttaaaaa gtatcttgtt agtattatag
      961 attagaagca gcttagcttc aaggtcacta tgtgagaaaa gaataacttt ttgattagta
     1021 tatcaaaaaa gcttgtctat gaaggtatct catagacaag ctttcttctg tatttgtgag
     1081 aggagtaggc acaaggtctc aagagataac cagcatcaag gtgcttgacg aacctcttga
     1141 accagtccta cttataagta atcgaaccat atgggctcag ggttgatcct gagatgttga
     1201 cattgatttc tttagacagt ttcacatctc tttcgtcgat cacttttcgc caccattccc
     1261 aagctaagcc ggtgcactct ctggccatga tacggatatt tcgtgaatta gctcctagtg
     1321 ggataactgt gctaaatggt gatgtcttac tataccagtt gttgtcccaa cgtcgttttg
     1381 taatcacttc ttttcctttg tcatcataat tgatttcatc ccaaaggatt tcatattgag
     1441 caacatacgc acctcgatga gacaggttaa tttttccact agtgtactcg gtagatgttg
     1501 tttcaacgta ttcagttctg ttattgacac ccgcaatttt attattttta aggaaaacac
     1561 tggtgtatga aataggataa gctgggtttt ttctactgaa ggtagcattg tctttgataa
     1621 cgtttctaat aacatcaaag tcttttgtga ccaccttatt gtgctctgca gcatctcctc
     1681 ctaaaacgac agctgtaaat gaactatttt ctaagatatc agagtatttt ccattcgttt
     1741 taacatctgt tccttttaga gctgcactaa aggccgcttc aacatcatta cttttagaac
     1801 ttgtttctag tttgacaaaa acagttcgac cataggctac gttactcaca aagagtggcg
     1861 gagcttcatt gctgacacct tttcgttgca actctttaaa ggtcactgat ttatcaaaca
     1921 catccgcagg attattagga aggtttgctg atacggtgta aaagatttgc ttgtatgctg
     1981 caatcatcac cttcttttca ccttttgaaa tcgacttgaa atcaatgcct aaagtaccat
     2041 ctaagatttt gctattaaca tttagagctg cttcaatctg tgacttagaa tataccattg
     2101 attcagtata ttgtgttctg gcaggaagcg tattaccacc agaataatta tcatgccatt
     2161 ggttaacaag attatcaata gctgttgaaa cattggcata ggtagggtca ttgacctcaa
     2221 ccgttgcttt gtctcccata cctggtaaat caatatggat tttttgtggg tttcgcttgg
     2281 tgaccaccgc gtctggtttg ttttcggtaa aacctttatc agccagctga agggctgctg
     2341 gataggtcat atcagtgaca gagtcaataa tggaaatatc gactggtgta gtgttgatat
     2401 tttttttctt tctttcaatg acaataaatt tatcagcttt cttaacgcct tctttaggaa
     2461 caaaattttc aatggtttca ccatttttag caagtacttc aagctcatta taatttagtg
     2521 aataaatctt gtcattgatt tcttcagtgt gatcttcttc gctctttttt ttgtcttctg
     2581 actttttttc ttctttttct gcagattcta gtggcatttc tttgggagca agcttaatca
     2641 tatcgttaga gttaagcata tcatccattt tctgacctgc tttttcgata gttagctcac
     2701 tactttctgg ttttggttgc tcatttgtcg ttgtggtttc tgtactagca gtgttttgtt
     2761 tgttcgattc agcattagca gtaacaagat taccaatgat aagagctgcc gttagtagcc
     2821 cagcgacgcg actgtatttt ttaaatgttt ttttattaga catgtccttc ataccttttt
     2881 atcattctaa aatgtttcta ttgttctttc gaccatatca agcatagtat cttgatagtg
     2941 agcaaattct cctaatttgt aatgtgccat agcctctcta atatgcgcat cttttaacgc
     3001 tttggttata ttagctagta tgctttgacg gtttaaataa aatggcaagc tttcgtaata
     3061 atttaataac atgttttcag cgtttagggt ttgaaccgct tggtaaatgt ctaatgcttg
     3121 accgctattt gacatttcta ataatgcttc ttcgatgtta gctttcaatt gggtatagcc
     3181 ttttaggaaa gcagggtaaa ccccattctc taaaataact tctggatcag aaatatctcc
     3241 catataagag tctcttctag tgatacgata ctctttgtca ctacctatca agtatttttt
     3301 taaatcatta acagttactt ctttttgaaa cattttttgg taatgttcta aaccctttgg
     3361 caccttatac atattgttac ttcctatctt gcattctatc ttgcattttc ttaattttga
     3421 agcgaccagt tactttttta tggtcaccat ttggagcaat gatggtagga tcaataattt
     3481 caaaatagcc tttggcatca atctctgcta agacagcttt agattttaga tctttaacac
     3541 taatcgatgg gaaaattaat tcactttctg aattttcaat gtgttttcct gaccacgttt
     3601 ggcaaccagg aatgttgagc gaatttgctc caggagtatc tccgctagct gttcgaatat
     3661 agcctagctt atcaatgttt tttgttagtt cagaatcgta ctcatactga taaaccccat
     3721 cgctatatct tccgctatcg acacctttta tattttcaat ttggtcagct gattttaact
     3781 taccttgatc agtaaagagc aatgccatat cttcagcaaa acttgtcaca aaaagaccgt
     3841 tttcttgtcg accaatcgtt gactttcttt tagcatcatc aaaagctaac atcaaatctt
     3901 ttttacttgt aacgtgagaa gctccgcttt ctttgtagac ttgttttagt ttttcaacaa
     3961 actctttttt gtagtggtat ttgcggctaa cgtacatccg gtaatactca gcacgtgacg
     4021 caacagcaga catctgaaca gctttatcag catatttgtc ggcagtttca ataaactcat
     4081 ctgcttgttt atctgttacc catttatctt taatttcttt aaactttttt tcaaaaagat
     4141 cgcgttccat attgccaaaa gtgacctctg acaaggctaa aatatccttg ttttgtccct
     4201 gatggacctc tgttacctca atatcaatac gatcaacatc taatttttta tcaatctcaa
     4261 tgtgatgcgg ttttttagtg taggtgtcgc ctaattcaat tttttgaaag aggaccaatt
     4321 tattcccgtt atagtattta acatcagcct ttgcaattct atttttcttg tagtaattct
     4381 ccttgctaga tgtatctcca ttaacaatta aaattctccc aatacgcatc ggggaagcaa
     4441 acttgtagga aagtacctca ccaacaccac caccaggtga attttcctcc cagacagtac
     4501 tcattgagcc gtctaatgtg tgcatgacat gattgtcttc tttagaggaa gttgcgttag
     4561 cttccataac tttggtcgtt tcatatttga catcgctttt tttattttct ttgccactaa
     4621 cagcgtacgt tgtgctatta gctaataaac tgctacccat tgctccagcc aaagcaatag
     4681 caacacttgt ctttgcgaca atatgagcta atgttacttt tttgtttctc atgtaaacca
     4741 ccttatatta tttttaagta aactatacat tagcaaatag tttttgtcaa tgcgatttat
     4801 taacatcgtt ttagactttt ctatcaaaaa aatgaacaca aaaaaacaag tagccaactg
     4861 ttacaagttt caaggggata tttccatctt attatcacag aaccttcaca tctgttctat
     4921 ttaaaccaga ttaaaagtac tagttaaata tttgatagta gcaccgttat cagtaataga
     4981 tctgttaatt atctctgatt acaatgatgt taacgatagt catacctttg tttgtacaca
     5041 cgcttcactt ccaactaaaa aaggtctgat ggtaatattt ataccatcag acctttgaga
     5101 gcctatagtt ctgcaatttg ataaagttca acttctgcta ctgtttcaga gccaaacatg
     5161 ttgagcatca gcttcacttt gttgttttcg atttctacca cacgaccttc ttgtcccata
     5221 aaggcaccgt cgatgatctg aaccacatcg ccttctttga tattggtatc aaagacatca
     5281 atggtttgac ccatagacaa caagatagca cggatttctt cttcaagaag tggtgttggt
     5341 tttgatcggt tcccgtgtga tcccacaaaa cctgtaacgt ttggtgtgtt acgcacaaca
     5401 aaccaagctt catctgtcat gaccatttca accaagacat accctggaaa gcgattttct
     5461 tcgatttctt tggtttgccc atttttttca acgttaaccg tttgtgtcgg aatttcgacg
     5521 cgcaaaatat tgtccaacat gttataggtt tgggcacgtt gcaagaggtt ttcttttact
     5581 ttgttttcgt agcctgagta agtttggagg acgaaccagc ccttatcaaa tgaatctagc
     5641 atgatgtttc ctttcattat aaaaaagccc tttttttggc ttttgcgctg ttttctatca
     5701 tcttcattat aataaaagac agggctgctg tcaacgtatc tgtggagcta cttgacacct
     5761 ttattaaaaa gtccaaagtc ggttagtgac gactctggac ggtattgtac agttttagtt
     5821 ttcgggaact gtgatggttc cttcgataat cgctttttta gcagcctcaa tagctttttt
     5881 agtgtcttgt gtcagagcat ccgttgtaag actaacacca ccttctttta aaccaaaagt
     5941 ggttatttga ccacctggga attggccttc tgaggttttt acggctactt ttactaaagc
     6001 ttttccgact tccttgatac ttgaggtcaa aacaaaatta gctgacttgc catcttttgt
     6061 ggtgtatttt ccatcttcac tttggtcacg gtcaacacca ataacccaaa ccttatcttc
     6121 ttctttacgt ttttcgttga tagacttagc ttcgctaaag acacccgccc ctgtgcctcc
     6181 tgctgcatga taaataacat ctgcgccttc agcgtattga gcagctgcaa tcgtcttgcc
     6241 tttggcagca tctgcaaaag agcctgcata agcaactctt actttgatgg tatcatctac
     6301 tgatttcaca ccagcttcaa aacctttttc aaagcgcttg acaacatctc cttccatacc
     6361 acctacaaaa ccaacttgct tggtttttgt cgttttagct gctgcaacac cggctaggta
     6421 tgccgcttca tggtctgaaa aggtgatact tgcaacattt ttttgacctt taatcacatc
     6481 atccacaatt gcaaaatggt tgtcaggatt gtttgcggct actttttcta cagcgtcatg
     6541 taatggaaat ccaatcccaa aaacaagatt ataaccattg gttactgctg actcaaggtt
     6601 tgtggtaaac tctgattcat tggctgattg gaaataatca tagccttttc ctttttcaag
     6661 tttattttct tttccccaag cttgcaagcc ttcccaggct gactggttaa atgacttgtc
     6721 atcaataccc gtctgattag taatcatagc gatttttaat gaattgtcag ttatttgttt
     6781 agcggagtta ttggtacagc ctcctaatgt gaatagggca gtcgaaacaa gaccaagtga
     6841 cattactttt ttgttcatgt gaaaatgaac ctcctaaatg tttaatccta aagtgatgtg
     6901 tcattgttaa cgttctactt atgctctaga acgaaaagtt tagtatatca aaaaaatcag
     6961 ttttatccta ctatttttgt tgaaaaatct ctttttcacc aaaatgaccg aacgataaag
     7021 tgagatcaac tggtaaatcc cattatctcc tcaaaaatca cctcttaagg agataagtca
     7081 acgacaaaac tcaagctact agtaatagca tgactaacta tacaatccaa caagcggtaa
     7141 aaagagctgg aaacgctatc caactctttg tcatactaga caccttagat agatgcccag
     7201 accgactcaa gaatattggt ctgttcacgt cctggaccaa ctgagaaggt tgaaatgcga
     7261 acgccaacca attctccaac acgacggaca tagttgcggg cattttcagg cagctcatca
     7321 aggctacgaa cacccgtgat gtcctcttgc cagcctggta attcttcata gattggtttg
     7381 caacgtttga gttgttcaag acttgctggg taatagtcaa tacgtttccc atcaaggtca
     7441 taagccacac aaatcttaac cgtatcaagc cctgaaagaa catcaattga attcagagag
     7501 aggttagtaa tacctgatac acgacgactg tggcgcatga caaccgaatc aaaccaaccc
     7561 acgcgacgtg gacgaccggt cgttgtcccg tactcatgac ccacttcacg aatgcgctca
     7621 cccacttcat caaagagttc tgtagggaat ggcccatcac cgacacggct tgtgtaggct
     7681 ttacagacac caactacttt gttgatttta cttgggccaa caccagaacc aatggttaca
     7741 ccaccagcaa ctgggtttga agacgttaca aatgggtaag ttccttggtc aatatcaagc
     7801 ataacccctt gtgcaccttc aaaaagaaca cgtttaccgg catcaagtgc atcatttaaa
     7861 attacagaag tatctgtcac atattgcttg atttcttgac cgtaagcata gtactcctca
     7921 aagatagcat caaaatcaag aggtgtactg tcatacattt tttcaaataa acggtttttc
     7981 tcagctaaat taatgcgtag gcgctctgca aagatatcct tatccagtaa atctgcgata
     8041 cggataccaa ctcgagcagc tttatccata taagctggac caatcccttt aatagttgtt
     8101 ccgattttat tgtcaccttt tgcatcttct tgcagttgat ccagctggat atgataaggt
     8161 aaaatgacat gagctcggtc tgaaatgcga aggttatctg tcgtcactcc ttcatcatgc
     8221 aggtacgcca actctttgac caaagatttg gggttgacca caacgccatt accaataacg
     8281 gaaatttttt gcgggaagaa aattcctgat gggatcaagt gaagtttaaa cttcttacca
     8341 tcaatcacga tcgtatgacc agcattatca ccaccttgat agcgtgcaat tacttctgca
     8401 tccgctgaca agaaatcagt gattttacct ttaccttcgt caccccactg ggtaccaaca
     8461 acaactacag atgtcatatc attgtcctaa ctttctgaaa cacttattct caataaaaat
     8521 cataggcaag ctaaccactg cttaaaaacc aaaaagaagg tcagatctgt aagatcagca
     8581 atgtagctct tgatatttaa agaaaacagg caggaatctc acctgctagc atatatctta
     8641 caacttatta tagcaaaaaa tgattttttc tgctagattt cttagcatta ttaaggaaaa
     8701 agagaaactc tttttttaac catgataaac aatcctaaaa aaacgaacat tataagagaa
     8761 aagcttttaa cctcgtttct cctaagcttt agcagggtta aacgattctc ttaaaacatt
     8821 agctcttttc tttcagaaat agtggtttta aggtaaaata atagtaagtt acctctatga
     8881 gatagccagt aaaaacatat cgaggaaata tcacatgatt agaaagtatg accgcacgag
     8941 cacaaaaaat aaatcactaa actggatttg gctaatcatt gcttttttta tgatttcaag
     9001 ctttataggt ggctcttctt ttactgagtc acttctagac attttgcctg ccatagccat
     9061 tggtggtaca ggttatgcta tttttcgtgt tagatctcat caaaagcggc tagcaaaggc
     9121 caagattgcc aaacaattag aagatctaaa agcaaaaatt cagctagctg atcgtaaagt
     9181 tcggctgtta gacacttatc tggcagacca tgatgacttc caatataacg tacttgctca
     9241 gcaattatta ccacaactta gtgacatcaa agcaaaagct attacgttaa aagaccagtt
     9301 agatcccaaa atttatcgtc gtatcaccaa aaaagccaat gacgttgaaa gtgatattac
     9361 ccttcaactc gaaacactgc agattgccac aactcttaac cctcagcctt tgaaaacccc
     9421 atctcctaat cttattaaca aagcacctga gctcaaaccg tattatgata atattcagac
     9481 agatcatcaa gctatccttg ctaaaatcca aggagccgac aatcaagaag aattgcttgc
     9541 gctgcatgaa gctaacatga ggaggtttga ggatatctta acaggctact taaaaatcaa
     9601 agaagagcct aaaaattact ataacgctgc tgctcgttta gaacaagcca aacaagctat
     9661 ccaacaattt gatgaagatc ttgacgaaac ccttcgtcga ctcaatgaaa gtgatcttaa
     9721 agattttgat attagccttc gaatcatgca aggtgctact caaagacgaa caacgcatca
     9781 ccaaaaagac taaaggagtc cctttatggc agattttaat tttgacattg accaaattgc
     9841 tgacaatgct gttgtcaaaa ccgataaaac caccgatatt atctcagact taccaactga
     9901 cacaaatggc caaatctctt tttttgagaa actaagtgct gaccagcaga cagctattac
     9961 tgccaaagcc ccggcattgg tagatacctt tttggcagac caaaatgcgc tacttgattt
    10021 tggtcagtct gctgttgaag gggtcaatgc aacggttaat catatcttgg cagagcaaaa
    10081 aaaattacag attcctcagg ttgatgatct tttaaaatca accaatcgtg aactcaatgg
    10141 ttttatcgct aaatacaagg atgccacacc tgtggacctt gataaaaagc caaactttct
    10201 tcaaaaactc tttaaacaaa gccgtgatac cctgcaagag ttttattttg attctcaaaa
    10261 cattgagcaa aaaatggata atatggctgc agcggttgtc aagcaagaag ataccttagc
    10321 tcgcaatatt gtctcagcag aattgttaat tgaagataat actaagtcca ttgaacattt
    10381 ggtaggcgtc attgctttta tcgaagctag tcaaaaagaa gctagtcagc gcgctgcggc
    10441 tttacaaaaa gacctaaaaa ccaaagacag tgctacacca gactatcaaa taaaagctga
    10501 tcttttggca agaactaccg aggtcatcaa cactctcgaa caacaacaca ccgaatactt
    10561 gagtcgcctc tatgttgctt gggcgacaac tccgcaaatg cgtaatctag tcaaggtctc
    10621 ttctgacatg cgtcaaaaac taggcatgtt acgccgcaat accattccaa cgatgaaact
    10681 atctattgct cagttaggca tgatgcaaca atcggttaaa tctggaatga ctgctgatgc
    10741 cattattaat gctaataatg ctgccctcca aatgctagca gaaacaagta aagaagccat
    10801 cccagcattg gaacaatcag cacaaaaccc tactttgtct ataaaatctg tgacaagtct
    10861 ggcagaaagt ttagtagcac aaaataatgg gatcattgcg gcgattgatc atggacgcaa
    10921 agaacgagca caactagaaa gtgctattat cagatctgcc gaaaccatta acgattctgt
    10981 caaactacgt gaccaaaata ttgtgcaagc acttcttagt gaaggcaagg aaactcaaaa
    11041 aacaattgat aaaacaactc gcgcataagc taaacatcac ctgtcacgct acttaaaaag
    11101 caagtcatca gttaggttaa tccctttgat ggcttgcttt tttactactt aacgaataat
    11161 cgccttgttt gatataacac ttatctttta tgatgttatt ttcataatac gaacaaggct
    11221 agcacgctcg gcctcttcta atttcaactc aatatagtga atcgtctctt ccagttgcgg
    11281 gataatagaa tactctaagc cattgacgcg acgtctagtc ttttcaatgt catcagccat
    11341 tagttgacaa gttttttcaa cttcagctaa ccgtaagagc ttttctaata gttcttttgt
    11401 cttttggata gtattgtcca tctcactatt tgagctgagg taactataag caaactcacc
    11461 ttgttcttgc tctcttgccg tattagactg gacatggaat ttaggcacat tgacactcat
    11521 gatattttca atatcaatcc ataaggtcac ttcatggact ggaacagcaa agagttcttc
    11581 taccattaga ctattttcag atgcttttgc caagacaaac tccttcatat tggcagcaag
    11641 ttctttttcg atagtttgac gaagctcatt gttttcacga attaaatcaa cgaagcggcg
    11701 catcaactca tcccgcttgt cctttaataa tttatgacct cttgtagctg tcttcaagcg
    11761 atttttcaag ttactaagtt ccatgcgtgt tggtttaaca tttaatcgtg ccattgatat
    11821 aactcctttc gtcaaggact tgaaattgca tggtcaactc aatcatttgc aacaaaaacc
    11881 tttgtcatcg tggtatctgc tttaggcaaa taacgatcta acatatcatc tttaatgcgt
    11941 tttaattcgg tacgtggcaa aatcgacaac aattcccaac ccaagtcaag actttcttca
    12001 atgctacggt tggtgtacaa tccttggtta atgtattctt cttcaaaccg gttggtaaag
    12061 gccacatata gtttatccgt ttcagaaagg gctgattcac cgagtacaac agccaattcc
    12121 ttagcttgtt ttccttgggc ataggctgca aagagctgat tcattgtagc agcatggtct
    12181 tgtcgagtct taccttcacc tgagccttta tcttttaggc gagatagcga tggcaagaca
    12241 ttaataggcg gtctaaaacc atttttatat agttcttgcg acaaaataat ctgaccttcg
    12301 gtaatataac ctgtcaaatc ggggataggg tgggtaatgt catcttctgg catggttaaa
    12361 ataggaattt gagtcactga cccttttttc ccgatcaagc gaccagcccg ttcatataag
    12421 gtggagagat tagtatagag atagccagga taacctcgtc tgcctggaac ttctctgcga
    12481 gcggctgata cttctcgtaa ggcttcacaa taatttgtca tatctgtcat gatgactaaa
    12541 acatgcatac ctttttcata agccaagtat tcggctgttg tcagagctat acgtggtgtt
    12601 gcaatgcgtt caatagcagg gtcattagct aaattgataa ataaaacaga tcgatcaatg
    12661 gcacccgttt cacgcaagtc attcataaag aattctgcct cttcaaaagt aatacccata
    12721 gctgcaaaca caaccgcaaa gttatcatca gagtttagaa cagttgcctg acgagcaatt
    12781 tgtgcggcca attcattatg aggtaaccct gagcccgaaa aaactggtaa cttttgacca
    12841 cgaacgaggg tattgaggtg atcaatagct gagatacctg tctggataaa ttcgtctggg
    12901 taatcacgag cgacaggatt gatcgcttga ccatcgatat caaggtattt ttcaggaata
    12961 agttctggac cgccgtctat gggatgcccc attccatcaa agatgcgtcc taccatatct
    13021 tctgatacgg ctaattccaa aggatgccct gtaaatcgaa ccttggtttt ggctaggttg
    13081 atgccagttg atccttcaaa tagctgtacc atagccttgt caccttggac ttctaagact
    13141 tgacctttac gcctttctcc attatgcaaa gtaatatcta ccaattcgtt gtagtgcact
    13201 cctgcaactt gatcaacaat catcaaaggg ccaaccactt cgctaaccgt ccgatattct
    13261 ttgagaacgc tcatctaaat tcctcccttt gctaaaacgt ggtgaatctc tttggtaacc
    13321 ttctgagtaa gccctttaat ctgctctaag ttttcttctg ggataaattt gctgcgtgcg
    13381 atgcgatcgc gtacctgagc agtgccttcc ataatctcat taaaataagc tcccaaagaa
    13441 agggctttgc tggcttcctc attaaaggtc aaaatattgg ttagcatggc ctcttgtttc
    13501 ggaaaggaag taaaggtatc caccgaatca aaggcatttt gctggagata atcctcccga
    13561 atttgccggg caacagccat ggtcaaacga tcttgttcag acagtgaatc aagccccacc
    13621 aagcgtacaa tttcttctag actggcttca cgctgcaaaa tagccatggc acgagttacc
    13681 ttgttggacc aattagattg ctgtttacgg tcaatatagc ttcctacatc atcttgatac
    13741 aatgaataag acgtcagcca gttaatcgct gggaagtgac gccgttgcgc aagaggcgcg
    13801 tcgagccccc agaaaacttt gacaatccga agggtgtttt gagtgacagg ctctgaaata
    13861 tcccctccag gaggagaaac cgcgccgatg gctgtaatgg ttccctcacg ttcttgactt
    13921 cccaaggtcc gaacacgacc agcccgttca taatattcgg caatacgact ccctaagtaa
    13981 gccgggtagc cttcatcacc aggcatttct tgtaggcgtc ctgacatctc gcgcagagct
    14041 tctgcccaac gtgatgtcga gtctgccatg atagccacag aatagcccat atcacggaaa
    14101 tattcggcaa tggtaatgcc tgtgtaaatc gacgcttcac gcgctgctac tggcatatta
    14161 gaggtgtttg caattaacac cgtgcgctcc ataatggact ggcctgtatt tgggtcaatt
    14221 aactctggaa actcattcaa aacgtcggtc atctcgttgc cgcgttcccc acaaccgaca
    14281 taaataacaa tatcaacgtt ggcaaattta gctatttgat gctgcacaac tgtttttcct
    14341 gccccaaatg gtccaggaac agcagcggca ccaccttttg taacagggaa aaaggtgtca
    14401 ataacccgtt gacctgtaac caaaggttcg actgggataa gcttttgagc aacagggcga
    14461 ctttgacgaa ctggccaagt ctgcatgagg ctaccttggt aaatggaacc gtcttccttc
    14521 ttaatttcat aaactgtatc tgttactgtg aagtcacctg catgaatagc caccaaggtc
    14581 cctgaaactt tataaggaac catgatacga tgctcaatga cagctgtttc ttgcacagtt
    14641 cctaaaatat cacctgcaac tacttcttga ccaacactta gcttgggaat aaatgcccac
    14701 ttagcctttc gatcaagact tgggatagcc acaccacgga ttaaaaagtc gctgtccgtt
    14761 gctttttgaa aacgatcaag cggtcgctga ataccgtcaa acatttctga aatcaggcct
    14821 gggcctaact cgaccgacaa aggacaacca gtagtcacta ctggttctcc tggaccgatc
    14881 cctgatgttt cctcataaac ctgaatagac gcttgatcgc ggcgcatttc aataatttct
    14941 ccgactaagc caagatgtcc cacacgacaa atatcttgaa tattagcttc ttgcatccca
    15001 gaagccacaa caagaggtcc cgaaacggtt attatttttc cttggttcac ctttttcctc
    15061 ctttattttt tccaaatcat actaactaat ctatactgtc aaggtttaat gtgactattt
    15121 tgaatagtca gcaaactatc ctatgcgttc agctgctaac ttcaccaatt ttcctgtcct
    15181 tacatttcat cagttagcta ctcataagcc tcaaacagag tataagtcct ctttgcctaa
    15241 cgtcttagtg ttataaaata ttttgaccga cagccttttc aaccatggct tgtatgcgtg
    15301 acaggcctat cccttgcgct ccttgatgag ttggtaatgg tatgactgct ggtagaactt
    15361 gattgtcata gtgagtgagg gcttctggaa tagctgcagc aatatcttct gtgatataaa
    15421 taataccaaa gtcttccatt gctagttgac gcaactggtt aatagcgtct tgtggtttta
    15481 ttactggaaa agtttgaaac ccaatcatct gaaaaggtaa aataacatct ctgttaccaa
    15541 ttaccccgac tttatacaat gttttggtca tgctattggt ctcatccttt ctatgacacg
    15601 ttctttgggt aaatcattag ctaaagcagc cgctaaaagc cgtagatttt ttacttcaaa
    15661 cgatttagcg agcaaaaaac gagcaagcac ataaggtcca tcgacttgat atttggcctt
    15721 cgcaaataaa tggtaaaggc attcatcaac aaggtattct aactcagttg tttgaagagt
    15781 gccttggcga agtttctctg aataaggttt tagggctgtc atatagctat caggtgttac
    15841 attttcaaac cacgtcatca gatccttatc atcttcaagt gtgataaatg ttttacttgg
    15901 tctacttgct tcatcagata ataactgcat catgaaactt attggtttat tttgcgattt
    15961 ggcccgtctg acggtaatca gattatacaa atcaatcaca ataagaacag cttgttggaa
    16021 aacaggatcc tctaatcttt gtgctatttg tttcaatgcc ttaaaataag ctaagtcggt
    16081 accaatttct aagacgcgta tgtcttgata atcttgatat tcggcccaaa tggactgggt
    16141 ttctgttacc acctcttttg gaaactcatc agaagtcatc gtcctaatca aatgttccaa
    16201 agccaccaaa ggcttgtcac caattggtag taataagtga ctcagatctg cttgactagc
    16261 tttagctttt aacaacactt tgacattatg ataggtgtaa cgcaaggtaa ataattgtac
    16321 tatgtcaggt tgaggggttt ctgcaaaagc ccagcgatag gtcttggtta gctcagccat
    16381 taagatagat tctagccgat ccaaatcgtt gagatcgtca acagttaggt gatagactga
    16441 ctggtgcaac agtcgagcta aggttgttgt attgggagcc tgcaatagct tatcaaattg
    16501 ctctttagtc aataactctt tttctttgac actgatagta gtgtttaatt ctagaaatgt
    16561 tggcatcgct agtcaaacct cctatatctc tgaaaaaagc atatcaatga tgcgggagct
    16621 ttcttcttta agaactgatt ctaaaaggta acggtataaa taattgtcat cgacttggtc
    16681 taaactgact aaaaatcctg cttcctgagg aattagctct tgattatagc ttaattgagg
    16741 aaacgctaat ctcagctcag cacaatcata agaactaaac ttttgccctg ttttttcgcc
    16801 aaaagtaact tgcaatggct gttctggata tttagacaag acttgcttca aaaaagccaa
    16861 ttcttcttcc ttagaaaagt tcgtcatctt ctccaaagaa gcttcaaata gctcctttat
    16921 actgtcttgt tttaatgcta gtagagcttg tctttcttga tttttgagtt gctgctgcgc
    16981 aacctgaaat tgctgttcaa gagcttttaa ctctttttga cgctttgctt ttaaatcgtg
    17041 tagtccttgc tgttgacgct ctttaaagtc agtatcaaga ctatctgtcg ctatctttag
    17101 gcactgttga ccttcttgat gtgctttttc aagaacgttt tgtcttaact gtgttatatc
    17161 attcacctaa tttccccctt ttgatagtgt tagtggtgtc accgtttttt aaaatctaga
    17221 taaggaccat accttatcca attttattta agagaataaa ggagacgaca aaggcaagaa
    17281 tagcataggt ttcaaccata gctgctaaaa tgacaccctt cataaaatct ttagggcgct
    17341 tagccaagat ttgcatacca gctacagaaa cattgccttg gtgcttggcc gaaaaataac
    17401 caacaatagc aattggaaga gataccaaaa agtaagctaa tccttggtta actgaaagtt
    17461 ctggtgtcag tttcatccaa attaaaatac caatagcaaa gccgtagatt ccttgactac
    17521 cgggtaacag ttgcaagata agcgcagacg taaatttttc gggttcttct tttaagagcg
    17581 cagctgccgc ttgaccacct ttaccgacac cataagctga gcccatgcca ctaagagcta
    17641 cggctaaaac aattcctaag gctgcgaaga aaacgccacc atgtgctgta aaataatgtg
    17701 ctaaatgttc catgttaatt gtcctccaaa tctgtttctt cattgacatt gacataatta
    17761 tcagctaact ttaaaggatt gaaagctttt cctccacctt cataaaattt tccaaaaaac
    17821 tctacgaaaa taagacgagc accatgcaca tatcctgaaa gcattgataa gaaaatattg
    17881 atcgcatgca aaagaataaa aataaatata cctactgtaa agcgcgtgac tggtggaaaa
    17941 atcccaacaa tcatgttaaa agccgctcca atactagctc cagaaagccc taatgccatt
    18001 agacgagtaa agctaaccaa atcacttaag taagagctaa tgccatataa attatataat
    18061 ccactaccta gacctaaaag gcttttgctt tttagtaaag aaacaaccaa aatcccaaag
    18121 gcattgccca atgcaagcca cttaccaata accgataagt aagcaaggcc agatacattc
    18181 ttaccaagaa taaataacag caggcctaat aaaataaaag tccaagctag acttgacgta
    18241 taggccgtag catatgcttt catccttact tgttgccatg ctcctaacaa aagcccaaat
    18301 ataagtgtaa caaacccaaa tagcaaagaa acaaccaaaa tagtaatcac atccgtttta
    18361 gttgataata atgccacagg taagtcaaaa ccaaagaaag aaccgtaaat aagcccccat
    18421 agggctactg aaatagctaa aatattaaag aaggtcacta accgtttact ggttttttgg
    18481 agattaaaaa agaccaatgc agctagtgtc acagcatata gcaacaaacc atagcccaaa
    18541 tcagctacca tcatgccaaa aaaagtaagg tataaaggag ctaaaaaagg tgtcggatct
    18601 ttttcttgat attttggcaa agcgtacatt tctgtaacca attcaaacgg tgcaatgtag
    18661 cggtgattac gtaacttaat gggaacatct tcccaatcgt ctggcgtgac atcgtaacta
    18721 tccagataaa acatatcacc tagtgtctta gtcattagcc cttttaattg gttaaccgaa
    18781 tcagcttcta tccacccttc taaggctatt agatgtcttg taccgagtaa ttgcttttta
    18841 gtttgctgcc gttgatactg actaaggaca tagtctattt caacctgtaa ctgtgccagt
    18901 atcttttgag actgcctaag ctcttttagt agactatctt tttcagctag ccaattcgtt
    18961 agtaactctt tgtttatacg aagttgttct gatggcagta gctgttcttt atataataag
    19021 ggtttaaact gatatttctg taaatgaatc gtatcttgtg cttgccaaaa taagacaagg
    19081 ccgtactcaa attctgtatg gtatacttcc tcaataaaaa gacctttgtg cttctttaac
    19141 tgatgataaa aatgatcatt tgctgtacta ggaatcgttc caactcttgc cgacaaaaaa
    19201 tgaaattgcg ctaagtcctg aggcaaaaca gtcaatgaac gccacttttc taactcctga
    19261 cagtattctg ttagctggtc tattttgttt tgcagctgct ctagtcgctc tatcttttgt
    19321 gacatcattt tcaaagccgt ttgagctgct tgttgtcgat ggcgttcttc gaactgttta
    19381 aacgaaagag atggcgtttt ttgtctcaag gctgttaact ttcctattgg tggtaaaaac
    19441 gctgataagc tagcaaggcc gttttctaac tcttgttggc tttgtaaaag gtattgtaaa
    19501 gcttcatcat ctagtagagg tttttgatgc gtcagatcgt attggacgat ttgtggcaac
    19561 ttgacgttgc ctttgttaaa agcatcttgc caatgtttta gttgcttcat gtcacgaact
    19621 tcaactaatt gagattgttg aagtgttttt aaaacaagat caagataatc tttttcaaaa
    19681 accattgcca gctttttcat ttgactaatg gccatatgtc tccaccacct tatccactaa
    19741 ctccctaaca acggttagct tatgctcttc taaaatggca tgaagagcta gctgttgcgt
    19801 cgctaacctc ttttttgaag ctttttgttc ttgacgatta gcttcaaaaa atacctcacg
    19861 tttagcttct aatagtagct gagtttcttg ttcgtactca agaaacagct gctttaactc
    19921 ttcttctttt ttgtcagcta gagctttttt cttagcatcg taactgtcat aaattgttct
    19981 agcctcttgt tcgatactat tcattagtga caatagggta ttcatcacat atcaactcct
    20041 tatctttatc tattgctaat tttttgactc actaggtact taaaaacaaa gctacaagaa
    20101 aggctacttt tgggggttaa tcaaccagcg actataagct agagctaaat ctctaaccta
    20161 tctactaaca gcttttttga gataggataa ttgcttgatt aagcgaacag catctttaag
    20221 ttaagcgctt tcaattctat ccataaaaac aatggcttag ttcagaaaaa ggttagctaa
    20281 aaacctgtca aagcagtaag atgacacaga actctaaagc tttgtattgc tgtctttcgt
    20341 gtgttatctt atacttcttt aggcatttag taaccttgat ctagttgacg gtaaggaacc
    20401 tttaccagcc aatagccaag ccaatgtgat aacagtataa ctgctatctt gttaaaagtc
    20461 ctgtcaaaaa gatgtttatt agtttctcgc ttgtaattct tgttttgtta ctaaacggta
    20521 atttttaaag acagaacctc caatataacc cacaattaag ctgataataa tacatccaag
    20581 ggctgttatc aacactttag cgacaggatt ataggcaaac atcaccgcaa aaccggcaat
    20641 cggagtagct gtccctggcg tatcattaac caatttcatt agggcgatca aaacaccaca
    20701 tgcagcacca ccaacaaaat tagtcacata aatcggaatg ggattggctg acgtcacatc
    20761 tgcctgcgtt agtggttcaa tagcaaaagc aatattgtct tttctttcac ccaattttaa
    20821 tcggtaaaaa agaacaccat tcataaaaga agagccaaag acagataagg cacctatagc
    20881 catcggaata cctgtcagac caagcatggc tgtcaaagcc attgatgaca gcggagctgt
    20941 tgctacaaca acaatcgtac cgcctagtat cattcccatg ataatagggt tgctattagc
    21001 acttgaggtc aggatgtctc caatagtcaa taaagtacta ttgatcactg gcgtaattaa
    21061 ctgcgccaag aaacgagtta atggtgcacc gacaataata atcgagatta aatcaagccc
    21121 accaggaata tttttttcca tccacttgat taaaaaagat accacatacc cagccacaaa
    21181 acctgggata atacctgttc ctgaaactga caaaccaatc aaaacggcat aacctggaga
    21241 cacaccaatt gcaatggcaa ccaagatagc agcagctacc cctgataatg agccatttgc
    21301 tccgcctact tcacttagaa actgaaagcc taaaacctta ccaaaaaaag aatcttgaaa
    21361 ggcttcaact aaaaaagagg cacaagctgc cgaagccaaa gctcccattg ccttggcacc
    21421 atgaggagcc ttataattaa acaagctaaa aatagctaaa acaagaatca aaagacttgt
    21481 tccaataata atatccataa tctattccac caatctatct cattacataa gacactataa
    21541 ctgcttcact ctggcaacag cttcgacttc aatcaaagct tgcaaaggaa gcgccgacac
    21601 ttgaaccgct gtccgaacag ggtagggctc ttcaaacaaa tcagtcatga catgatttaa
    21661 tatttccaca ttggtcacat ctgttaaata cacatttagc ttatagatat gattcaagtc
    21721 caacttttgc tctgccaaaa tagattgtaa attaacaaaa acctggcgac actgtgcctc
    21781 aaaaccatct gacaattgac ctgtcactgg attaaggggt aattggcctg cggtgtatag
    21841 aaaatgtcct tcaatcgtat aagttgaata aggacccatt ggttctgggt agctcttcat
    21901 atacgaacct cctcattgat aatatagtta aattttacag gaaaccgcat tcacagtcaa
    21961 taataaattc tctttttgat aaaaaattat tattaaggtg attggcaatt gttttttagt
    22021 ctaaaatgta ttactatatt tgtaaataag aagatagata taggaagttt gacatggata
    22081 aagaaacgct aaactactgg aaaacggtta tcacatttct tcacgatgtc ttaggtgata
    22141 actatgagat tattttacat gtgattgata aaaatgatat ctatattgga gaactcgtta
    22201 atagtcatat cagtggacgg tctaaacagt ctcctctcac cacctttgct cttgatttga
    22261 ttaccaataa agtctataag gaaaaagatt ttgtgaccaa ttacaaagca atcgttagtc
    22321 ctcagcacaa agaagttcgg ggatctactt tttttattaa agataaaaag ggtaatctcg
    22381 agggaatgct atgtattaac ttagatattt cagcctatca aggagtagct agggacctct
    22441 taaaacttgt caatctcaat ttagaacatt tcatcccaac tgctaaagaa cctaaaacag
    22501 tcacacccca gccagaagaa gctgttgaaa ttttaacctc taacatccaa gatattatcg
    22561 gtcaaattat tgatccctcg ttactgagac acaatgtaca tcttagccaa gatgtcaaaa
    22621 tagacattgt tgccaagctt tatgaaaaag gagtatttca actaaaagga gccgtctcaa
    22681 aagtcgctga tattctatgt atttccgaac caagtgttta ccgctacctt aaaaaaattg
    22741 aagccgatca ataaccaaat caccataaaa cgccagttaa aagtatttca gataagcatc
    22801 acaatctctg atataccgat gactgacgtt tttttactca atatgccttt atggcaagaa
    22861 aaggctacta gtcattaacc ttcagctatg cttagaggta cacctgtcgc agcttgaacc
    22921 tcatcaaaag taaacccttc actgatttca attacttgga taccatctgg cgttacttta
    22981 aacacaccca tttctgtgat aataaggtca accacacctt ttgctgttaa aggtaaggta
    23041 cattctttta ggagcttatg ctttccttta ttggtatgct ccatagctac gatgactttt
    23101 ttagcaccaa ctaataaatc catcgctcct cccataccag gaaccatttt gcctggaatc
    23161 aggtaattgg cgatatctcc attttctgca atttctaaag cacccaagac agtcaagtca
    23221 acatgaccac cacgtataat gccaaaagaa tctgcactat taaagaaagc ggctcctgga
    23281 taaaccgtca caggctgtcc tcctgcatta acgatagtcg aattcggatc atctgactta
    23341 ggacctagtc caataaaccc attttcagat tgaaaataca ctgtcatttc ttcaggcaca
    23401 aaatttgcta ctttggttgg tagaccaata cctaaattta ccaaagtacc atcttctaat
    23461 tctttggcca cacgctttgc aatacgattt tgaatctctt ctttacttaa tgcaaccgcc
    23521 ataactatac tccttcttta acaatgtaat ctacaaaaat gtgtggtgta tgaacgcatt
    23581 ctggttgtat ggtgccgact ggtactattt ctcgcacctg aacaattgtt gtttttgcac
    23641 aagctgccat caattgatta aaattatttt cagaaccact atactgtaaa tttcccattt
    23701 catcagcttt attagcaaat atcaaggtga catctgcttc aaatgggtat tctaagagat
    23761 aggtttttcc cttgatggtt tttgtttcct tgccttctgc cactaaggtt cctacccctg
    23821 ttggcgtgta aaagccacca atgccataag ctccgatgcg aattttttcg gcgaaggttc
    23881 cttgaggaat gagctcaatc gtcgtttcac cggcagtcat ttggcgccca gcttctttat
    23941 ttaatccaat atgtgttgca taaatggttt tgaattgatg attagccacc atttttccaa
    24001 caccgcgatc aacaaaacca gcatcattac agatcaaggt taaatctttt gtgcctttgt
    24061 caaccagtgc atcaattaac gcttctggtg ttccattagc cataaaacca ccgaccataa
    24121 tcgtgtcacc atctttaata tgactgatag cttctgctat tgctattctt ttattctctt
    24181 tcatacggtt accttctatc ttgttacgat gatactttga ccttggccac caccaataca
    24241 aagcgttgct aagccatgtc tcacatcacg cttagccatt tctgctaata aggtgactaa
    24301 aatacgagca cctgatgctc caatcgggtg acccaaagca atagctcctc cattgacatt
    24361 aactttttca ttgtcaaaac caaggtctcg tgaaaccgct aaagcctgag cagcaaaagt
    24421 ttcgtttgct tcaatcaaat caatgtcatc aattgtcaac tgagcctttg caagagcctt
    24481 tttcgtagca ggtattggtc cgcagcccat aatacttggg tctacacctg cacttgcata
    24541 actagtgatt ttagctaaaa taggaagccc taattcttca gctttttctt tactcatcaa
    24601 taaaattgct gctgctccat cattgattcc tgaagcatta ccagcagtta ctgtcccctc
    24661 atctttgata aaagcagggc gtaactttgc taacttatcc actgtcgttc caaatttagg
    24721 gtattcatct tgatcaacga gtaaaggttc acctttacgc tgaggaatag tgacaggtgc
    24781 aatctcttct ttgaaacgac ctttttctat ggcaagttgg gctttacgtt ggctatcagc
    24841 tgcaaaggca tcttgttctt ctctggtcag tccatactgt tgcacaatat tttcggctgt
    24901 tatacccatg tgataacctg caaaagcatc actcaaccca tctttaagca tggtatctac
    24961 tactttacta tctcccatac gagatcccca acgttggcct tgtaaaacgt aaggtgcttg
    25021 actcatgttt tcggtaccac cgactaaaac aatatcagcg ttgcccgttt gaatcttttg
    25081 agcacctaac tcaactgctt ttaagccaga accacagacc atatcaatcg taaaagcagg
    25141 ggtatcataa gaaagccccg catttagcgc tacttgccga gcaacatttt gacccagacc
    25201 tgcatgtaac acattcccaa agatcacttc atcaatggca tcacttttaa gaccggtatc
    25261 agctaaaatt tttgtaacaa ctgtcacccc caaatctacg gctgataaag atttaaagac
    25321 tcctccaaaa tttcctatcg gggtacgata agcagatgtg atcaccactt ctttagtcat
    25381 agaaacttct cctttttcat tattgaaacg cttacatttt tattatataa tgtgaactat
    25441 aataaacaaa tatcgttttt ttatatatgc tataactaaa cgttatcgaa agaggaaaat
    25501 atgaatatct caagcttggc ttattttatt gagattgtta attgtgactt taacttaacc
    25561 aaagcagccc aaaaaagtca tatttcgcaa cccgccctta gtagttatat caagactatc
    25621 gaaaaagaag aaggagtcgt tttattccag cgtcaaaaag ggcgtctagt tggactaacc
    25681 tcagtcggcg aacgcttttt tgaaaatgct aaggtggtta tcgcatcaca tgagcaatta
    25741 atggctgatt tgcggagtga atccaaaaaa tacaaaggag aaatttcaat cggtattcct
    25801 caactcatct ctggcattgt tttttctgag ctcattccaa aactcatcga agaaaatccc
    25861 gaagctaaat ttcatattgt agaaacgggt gcttatgaac tgcaaaaaaa actgtttcta
    25921 aaagaagtcg attttgcgat tttattaaca cccactacac tagatgctac tattgctgat
    25981 atcgacattc tcctaagaga tcaactctgc ctttatatga gtcacaaaac ctttcaagag
    26041 acgtttgata gtaatcctaa catctctatc aaagcattac atcgtcaaaa cttagtaact
    26101 tttgataaaa cctttatgat taatcatcag cttaccacct tatttgaaga agcaaatagt
    26161 cttcctgtag tcaaatgtta ttctgcctcg tgggattttt tattaaattg cacgcgctat
    26221 tcctcttacc taacaatcct accaaggcct attactcatt ttgctcatat ggacggttta
    26281 gtggaggtcc aactgaccga acatccaaaa tgggaagttg ttctagcctc tctcaagcac
    26341 aataaaacaa gccatttaaa acattatatc aagcacacca tcttagatta tttcatgaac
    26401 cagcgcttct aattgcttga ctcagacctt aaaaaaaaac gcctgaagga aacaatccca
    26461 agcgtttcat gtagttattt cctaaaatac aaataggtaa atgatggcaa tacctgtgac
    26521 caccaagaca caaggaatag tataagccac aatttgattg aattttagtt tgaagtttga
    26581 gagataagat aaggcccaaa aaggaactat caagtttgtt gtggcatctc ctaattgata
    26641 agcctgcaaa atcttaccta gatcattacc gacttcgata gttgcaggga cgatatatgg
    26701 agcttcaatc acaaattttg agcctcctga tggtaccgca atattcagca aagccgagta
    26761 aagataagca aagaccggcc atgttgtccc atttgaaata gcgacaaaga aatgggaaat
    26821 ctctagtcct agacctgtac tttgaatgat tccaaaaata ccagcgtaaa atggaaactg
    26881 aagcaccaag ccccaagatg acatgacccc atctttgaat aaggaaccat aatattctgg
    26941 tccttgttga ccacaaagaa gcaaccctaa gcctaaaaat aaaaagttaa aactattgag
    27001 atctagacca gacaagccca ccaccgaaaa atgataaagg ctatagctaa caatggcccc
    27061 accaacaaga tacatcacta agcgagatgc gttcatcttt tcagcaggtg tgtttcttgc
    27121 aataacaaca tgtgaagcgg tatcaagact atggtgatag atctcatcat caagttccat
    27181 gattttagtt tcatcttttg ggtgcgccaa taaagcaaag caaagcggta ccagcataca
    27241 caccactaaa agactgataa atggaagatt caaaactgat tctgtcagag gaacactttc
    27301 aggaacaacc tgtttataag catctgcgac actatttcgc aaataatctg gagtagccga
    27361 ataaagcacc gcagcaccag ataaaccaga agtagctggt aaaaaggtaa aaaataaagt
    27421 agcaacaaac aaaggtgtat gcaccttaat ccctttttgc ctagcctgaa caagcaactc
    27481 tttcccaaac acaatggcaa ccatcatacc aagaccccag tgtaaaaagc caaagataga
    27541 acctactaca atagaaaaaa taaaaatctg agtcctggtt tgaggcagtt tggcaaggga
    27601 cttaaacatt ttatgagcag gtggagaact cgctacagca ttccctgtcg caagtaaaat
    27661 agtcatctgc atggcaaagg acaacagccc ccaaaaacca tttacccagc catagataat
    27721 cccgcccgtc ttttccgtac caataaaggg actatccgtc atcaacacag acatagtaac
    27781 gactaaaaag gttaaaataa aacagataaa aagcgattct ggcatccatt tcataaagcc
    27841 atcaatatag cgatccataa accttttttt ggttttgatg ttattcatgt tgtcaattcc
    27901 tttcacattg tccacaagca cttttgttct tttgatcagt aacctatcga taatactaat
    27961 aagcgtttct ttagttcatt tagttatcaa taagtttaaa cccacaaaaa acacttttgt
    28021 tcccttttta ttatgacaac gttttcatag acaaacaact atgatttttt tattgcttcc
    28081 ataaccctta tttatagcaa aaagaatgac gcttcactca tc
//
LOCUS       DATXIG010000017        27832 bp    DNA     linear   BCT 28-DEC-2023
DEFINITION  TPA_asm: Streptococcus pyogenes strain MGAS11113
            SAMN08555799-rid19682583.denovo.017, whole genome shotgun sequence.
ACCESSION   DATXIG010000017 DATXIG010000000
VERSION     DATXIG010000017.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN08555799
            Sequence Read Archive: SRR6740495
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pyogenes
  ORGANISM  Streptococcus pyogenes
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 27832)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 27832)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-OCT-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-OCT-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001918058.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001918058.2
            Genome Coverage       :: 368x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/12/2023 11:23:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 1,806
            CDSs (total)                      :: 1,769
            Genes (coding)                    :: 1,722
            CDSs (with protein)               :: 1,722
            Genes (RNA)                       :: 37
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1, 1 (5S, 23S)
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 47
            CDSs (without protein)            :: 47
            Pseudo Genes (ambiguous residues) :: 0 of 47
            Pseudo Genes (frameshifted)       :: 30 of 47
            Pseudo Genes (incomplete)         :: 20 of 47
            Pseudo Genes (internal stop)      :: 9 of 47
            Pseudo Genes (multiple problems)  :: 11 of 47
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..27832
                     /organism="Streptococcus pyogenes"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN08555799-rid19682583.denovo.017"
                     /strain="MGAS11113"
                     /serotype="Emm28"
                     /isolation_source="normally sterile site"
                     /host="Homo sapiens"
                     /db_xref="taxon:1314"
                     /country="Finland"
                     /collection_date="2002"
                     /collected_by="National Public Health Laboratory"
     gene            complement(280..2085)
                     /gene="pepF"
                     /locus_tag="VC122_000788"
     CDS             complement(280..2085)
                     /gene="pepF"
                     /locus_tag="VC122_000788"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002983996.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oligoendopeptidase F"
                     /protein_id="HEP5640730.1"
                     /translation="MTDNRSHLEEKYTWDLSTIFATDKDWEAEVSDLATEIDAAKDFS
                     GHLLDSSANLLTITDTYLELARRVEKVYVYAHMKNDQDTTVAKYQEYQAKASGLYAKF
                     SEVFSFYDPEVMALSQEDYQAFLAETPDLKRYHHFFDKLFQTRDHVLSQAEEELLAGA
                     QEIFNGAEETFSILDNADIVFPVVKNDKGEDVELTHGNFISLMESKDRSIRQAAYEAM
                     YSTYEQFQHTYAKTLQTNVKVQNYKARVHKYDSARQAAMAANFIPEAVYDTLLETVNK
                     HLPLLHRYLKLRQEVLGLDDLKMYDVYTPLSETDLAIGYDEALEKAEKVLAVFGKDYA
                     DRVHRAFTERWIDVHVNKGKRSGAYSGGSYDTNAFMLLNWQDTLDNLYTLVHETGHSL
                     HSTFTRETQPYVYGDYSIFLAEIASTTNENIMTEALLNEVQDEKERFAILNHYLDGFR
                     GTVFRQTQFAEFEHAIHQADQKGEVLTSEYLNQLYADLNEKYYGLSKKDNHFIQYEWA
                     RIPHFYYNYYVYQYATGFAAASYLADKIVHGTQDDIDHYLAYLKSGNSDYPLEVIAKA
                     GVDMAKGDYLEAAFKVFDERLTELEVLVSKGIHKS"
     gene            complement(2098..3060)
                     /locus_tag="VC122_000789"
     CDS             complement(2098..3060)
                     /locus_tag="VC122_000789"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002995771.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="competence protein CoiA"
                     /protein_id="HEP5640731.1"
                     /translation="MTKILTALDGKNQLISLVTQPISTKPPFCCPACKSPVRLRQGTI
                     RRPHFAHVQLAHCQFQAENESEEHLTLKAKLYTSLVRTEAVCIEKYLPELQQIADLWV
                     NDKLALEIQCSPLPVERLKKRTKAYQEKGYPVRWLLGRKLWLNTHLTALQKQFLYFSS
                     SLGFHLWELDAAANLLRLKYLIHEDLFGKVSYLTKTISLDHNIMEMFRLPYQQEILYS
                     YQKKMTVNLSKRIQRALLARHPKWLRRQEKAYLSGYNLLMLTTDAFYPQWRPVQSSSG
                     FCQIKGNLRPYYESFKVYYKKEKDKKVQTLFSPKYYVKMDSNRK"
     gene            complement(3355..4071)
                     /locus_tag="VC122_000790"
     CDS             complement(3355..4071)
                     /locus_tag="VC122_000790"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011528761.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rRNA pseudouridine synthase"
                     /protein_id="HEP5640732.1"
                     /translation="MRLDKLLEGTKVGSRSQVKKLIKAQGVWVDHMPARNGRQNVDPG
                     LQLIEVTGQRVTHPKHSYIILNKPSGVVSAKKDTNYLTVIDQLAEEDKSPDLYPVGRL
                     DRDTEGLVLLTDNGPLGFRMLHPIHHVSKTYLVTVNGLLAEDASDFFAAGICFPTGEQ
                     CQPAQLTILKADTDQSQASLTISEGKFHQVKKMFLTYGLKVTSLKRTHFAGLELGHLA
                     SGEYRYLTASERQLIKTYLD"
     gene            complement(4190..4894)
                     /gene="nagB"
                     /locus_tag="VC122_000791"
     CDS             complement(4190..4894)
                     /gene="nagB"
                     /locus_tag="VC122_000791"
                     /EC_number="3.5.99.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012679214.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucosamine-6-phosphate deaminase"
                     /protein_id="HEP5640733.1"
                     /translation="MKIIRVQDQIEGGKIAFTLLKDSLAKGAKTLGLATGSSPISFYQ
                     EMVKSPLDFSDLTSINLDEYVGLSVESDQSYDYFMRQNLFNAKPFKKNYLPNGLATDI
                     EAEAKRYDQIIAEHPIDFQVLGIGRNGHIGFNEPGTSFEEETHVVDLQESTIKANSRF
                     FTSIEDVPKQAISMGIASIMKSKMIVLLAFGQEKADAIKGMVFGPITEDLPASILQKH
                     DHVIVIVDEAAASQLD"
     gene            5096..6124
                     /gene="queA"
                     /locus_tag="VC122_000792"
     CDS             5096..6124
                     /gene="queA"
                     /locus_tag="VC122_000792"
                     /EC_number="2.4.99.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010922429.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA preQ1(34) S-adenosylmethionine
                     ribosyltransferase-isomerase QueA"
                     /protein_id="HEP5640734.1"
                     /translation="MNTNDFDFELPEELIAQTPLEKRDSSKLLIIDHRQKTMVDSHFD
                     HIIDQLNPGDALVMNNTRVLPARLYGEKPDTHGHVELLLLKNTQGDQWEVLAKPAKRL
                     KVGSQVNFGDGRLKATIIDELEHGGRIVEFSYDGIFLEVLESLGEMPLPPYIHEKLED
                     AERYQTVYAKENGSAAAPTAGLHFTTDLLKKIEAKGVHLVYLTLHVGLGTFRPVSVDN
                     LDEHDMHSEFYSLSEEAAQTLRDVKQAGGRVVAVGTTSIRTLETIGGKFQGDIQADSG
                     WTNIFIKPGYQFKVVDAFSTNFHLPKSTLVMLVSAFAGRDFVLEAYRHAVDEKYRFFS
                     FGDAMFVN"
     gene            6131..7351
                     /locus_tag="VC122_000793"
     CDS             6131..7351
                     /locus_tag="VC122_000793"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002989189.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3114 domain-containing protein"
                     /protein_id="HEP5640735.1"
                     /translation="MKLLGKWLFFLMFLTFCVSFWQKDYQRIAFEKEHKLYQNLVAPF
                     YQNYPKLRELQLSEAIKLRHDLLDYVRKLDKDGWSYKAIQKGYLEQLAVRGNSYHFEQ
                     LYSRIRLIGSPDFQKLWQQEEMAQTPQEAQKRLQLLLTYLKMPDELTGQSKQTQQVLA
                     QLSPKLSPTDPFWDQLSTLIQAYYVNLEHIPYSRFNRKIHQLRYLISTQQTEWVRSHY
                     GNKGKTDADALAKYLATLEDDDYALYESSRYHNKVASILDANGNHQAVYTDNIPQANY
                     KILIHFHSEFILSESGQFLVALDPDNLTRNSIVNGSSFNYGNQNDDLHRLLDIDPILL
                     FDPAFIEKAINSPDATFLVPDLEQQRDKHNPIYSRNGKSSKQLTRAAVKKFKKLLHHY
                     QETRGDNKTSKTQH"
     gene            complement(7465..8055)
                     /locus_tag="VC122_000794"
     CDS             complement(7465..8055)
                     /locus_tag="VC122_000794"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012560800.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine hydrolase"
                     /protein_id="HEP5640736.1"
                     /translation="MTERFDITETDYEYDQEHHAYVAQFNGAMSIPDMQEYSLVYSEN
                     TPAYALAERLGGMNKAYQLFDRYGKVSGAITTIDRNGNKITTAYYLQGLDYLWQHQDK
                     YKDILYYIGESFPDLYYKTYLPHVKVYQKPGYVREALNVGAIVCEESPYLITLYSSGL
                     GGATQASEEVNGLGYVQLVQLTYVINEWHRVNHNMI"
     gene            complement(8052..8300)
                     /locus_tag="VC122_000795"
     CDS             complement(8052..8300)
                     /locus_tag="VC122_000795"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010922432.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEP5640737.1"
                     /translation="MDADLAQMDALGLYYDDNHLSLVNTVKVYLKEFGIGESQIAFSY
                     KDLNSGRTAAMNDHQPMIAGSTYKLPLNMLVVDKANYP"
     gene            complement(8285..8511)
                     /locus_tag="VC122_000796"
                     /pseudo
     CDS             complement(8285..8511)
                     /locus_tag="VC122_000796"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010922433.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            complement(8678..9283)
                     /locus_tag="VC122_000797"
     CDS             complement(8678..9283)
                     /locus_tag="VC122_000797"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015057806.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="superoxide dismutase"
                     /protein_id="HEP5640738.1"
                     /translation="MAIILPELPYAYDALEPQFDAETMTLHHDKHHATYVANTNAALE
                     KHPEIGENLEELLADVTKIPEDIRQALINNGGGHLNHALFWELLSPEKQDVTPDVAQA
                     IDDAFGSFDAFKEQFTAAATGRFGSGWAWLVVNKEGQLEITSTANQDTPISEGKKPIL
                     ALDVWEHAYYLNYRNVRPNYIKAFFEIINWKKVSELYQAAK"
     gene            complement(9380..10420)
                     /locus_tag="VC122_000798"
     CDS             complement(9380..10420)
                     /locus_tag="VC122_000798"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002995622.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit delta"
                     /protein_id="HEP5640739.1"
                     /translation="MIAIEKIEKLSKENLGLITLVTGDDIGQYSQLKSRLMEQIAFDK
                     DDLAYSYFDMSEAAYQDAEMDLVSLPFFAEQKVVIFDHLLDITTNKKSFLKEKDLKAF
                     EAYLENPLETTRLIIFAPGKLDSKRRLVKLLKRDALVLEANPLKEAELRTYFQKYSHQ
                     LGLGFESGAFEQLLLKSNDDFSQIMKNMAFLKAYKKTGNISLTDIEQAIPKSLQDNIF
                     DLTRLVLGGKVDVARDLIHDLRLSGEDDIKLIAIMLGQFRLYLQLAILAKEGKSEQQL
                     VTSLSEILDRRVNPYQVKYALKDSRTLSLAFLTGAVKTLIETDYQIKTGLYEKSYLVD
                     IALLKIMTHSQK"
     gene            complement(10491..12733)
                     /locus_tag="VC122_000799"
                     /pseudo
     CDS             complement(10491..12733)
                     /locus_tag="VC122_000799"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002989173.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DNA internalization-related competence protein
                     ComEC/Rec2"
     gene            complement(12714..13376)
                     /locus_tag="VC122_000800"
     CDS             complement(12714..13376)
                     /locus_tag="VC122_000800"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011184716.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ComEA family DNA-binding protein"
                     /protein_id="HEP5640740.1"
                     /translation="MLDELLIKGKSFLRERYFLPYLISLLLGLFLILSFSFLFWGNRQ
                     SKAAVPALREISPVKQQVSEEKKEIQEDSSILVDLKGAVQKEGVYKLTASSRVRDVIE
                     LAGGLTSEADKHAINFAEKLTDEQVVYVPKQGEEISVLPRSLVSGKKETASKDQSKVH
                     INKASLEELQHIPGIGAKRAQDIIDMRDKLGGFKALEDLRQVSGIGEKTLEKLKDDIF
                     LD"
     gene            complement(13576..14316)
                     /locus_tag="VC122_000801"
     CDS             complement(13576..14316)
                     /locus_tag="VC122_000801"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014635566.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="1-acyl-sn-glycerol-3-phosphate acyltransferase"
                     /protein_id="HEP5640741.1"
                     /translation="MFYAYLRGLVVFLLWVVNGNAHYHHEEKMLDASENYILVAPHRT
                     FWDPVYMAFAARPKQFIFMAKKELFANRLFAWWIKMCGAFPIDRDKPSPDAIRYPVNM
                     LKKSNRSLLMFPSGSRHSQEVKGGVAVIAKLAKVKIMPAAYQGPMSVKGLLAGERVDM
                     TFGNPIDVSDIKRMNDEGIAEVANRIQAEFDRMDDELAPFQPGKARNPLTYLYRLPLG
                     LVLVVVLLLTILFSYIASFVWNPDKHRH"
     gene            14356..15210
                     /locus_tag="VC122_000802"
     CDS             14356..15210
                     /locus_tag="VC122_000802"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002983961.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA1(Val) (adenine(37)-N6)-methyltransferase"
                     /protein_id="HEP5640742.1"
                     /translation="MKADFSKVKITLQTFTSSLFYVKITGMIKAILKEGERIDQLFSS
                     DVGIIQNKDVFSYSIDSVLLSRFPKMPSKGLIVDLCSGNGAVGLFASTRTKAAIVEVE
                     LQERLADMGQRSIQLNQLEDQVTMICDDLKNLLNHVPRSGVDLMLCNPPYFKSHESSK
                     KNVSEHYLLARHEVTTNLEEICQVARHALKSNGRLAMVHRPDRFLEIIDSLRANGLAP
                     KRVQFVYPKLGKSANMLLIEAIKDGSIEGMTILPPLVVHKENGEYTDHIFEIYFGAAS
                     KGKPNHDH"
     gene            15200..15478
                     /locus_tag="VC122_000803"
     CDS             15200..15478
                     /locus_tag="VC122_000803"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011017953.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GIY-YIG nuclease family protein"
                     /protein_id="HEP5640743.1"
                     /translation="MTTKKAYMYVLECADKTLYTGYTTDLKKRLATHNAGKGAKYTRY
                     RLPVSLLYYEVFDSKEAAMSAEALFKKRKTRSQKLAYIATHQKEKKNH"
     gene            complement(15502..17502)
                     /locus_tag="VC122_000804"
     CDS             complement(15502..17502)
                     /locus_tag="VC122_000804"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014612436.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="potassium transporter Kup"
                     /protein_id="HEP5640744.1"
                     /translation="MSDSHLTAFDKASKAGFIIALGIVYGDIGTSPLYTMQSLVENQG
                     GVNQVSESFILGSISLIIWTLTLITTIKYVLIALKADNHHEGGIFSLFTLVRKISPWL
                     IIPAMIGGATLLSDGALTPAVTVTSAIEGLKAVPGLSHIYQNQTNVIITTLVILIVLF
                     GIQRFGTGFIGKIFGPVMFIWFSFLGVSGFFNTLGHLEIFKAINPYYALHLLFSPENH
                     RGIFILGSIFLATTGAEALYSDLGHVGRGNIYVSWPFVKMCIVLSYCGQAAWILANKH
                     SGIELNPFFASVPSQLRVYLVSLATLAAIIASQALISGSFTLISEAMRLKIFPLFRVT
                     YPGANLGQLYIPVINWILFAVTSCTVLAFRTSAHMEAAYGLAITITMLMTTILLKYYL
                     IKKGTRPILAHLAMAFFALVEFIFFLASAIKFMHGGYAVVILALAIVFVMFIWHAGTR
                     IVFKYVKSLNLNDYKEQIKQLRDDVCFDLYQTNVVYLSNRMQDHMIDRSILYSILDKR
                     PKRAQVYWFVNVQVTDEPYTAKYKVDMMGTDYMVRVNLYLGFRMPQTVPRYLRTIVQD
                     LMESGRLPKQEQEYTITPGRDVGDFRFVLIEERVTNARQLSNFERFIMQTKASIKHVT
                     ASPMRWFGLQYSEVTLEVVPLILSDVLKLPIKELVPVEDSEA"
     gene            complement(17629..19248)
                     /locus_tag="VC122_000805"
     CDS             complement(17629..19248)
                     /locus_tag="VC122_000805"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002989158.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase"
                     /protein_id="HEP5640745.1"
                     /translation="MKFTEFNLSQDIQSAVVTAGFEKASPIQEMTIPLALEGKDVIGQ
                     AQTGTGKTAAFGLPTLNKIRTNENIIQALVIAPTRELAVQSQEELFRFGREKGVKVRS
                     VYGGSSIEKQIKALKSGAHIVVGTPGRLLDLIKRKALILDHVETLILDEADEMLNMGF
                     LEDIEAIISRVPADRQTLLFSATMPAPIKQIGVKFMKDPEHVQIKNKELTNVNVDQYY
                     VRVKEQEKFDTMTRLMDVNQPELSIVFGRTKRRVDEITRGLKLRGFRAEGIHGDLDQN
                     KRLRVIRDFKNDQIDILVATDVAARGLDISGVTHVYNYDITQDPESYVHRIGRTGRAG
                     KSGESITFVSPNEMGYLSMIENLTKKQMKPLRPATAEEAFQAKKKVALKKIERDFADE
                     TIRSNFDKFKGDAVQLAAEFTPEELALYILSLTVQDPDSLPEVEIAREKPLPFKYVGG
                     GHGNKNGKGGRGRDNRNRGDRRGGYRGDRNRDERDGDRRRQKRDKRDGHDGSGNRDFK
                     RKSKRNSKDFFNKEKKSSAKNTGFVIRHKGE"
     gene            complement(19555..21099)
                     /locus_tag="VC122_000806"
     CDS             complement(19555..21099)
                     /locus_tag="VC122_000806"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011681503.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor 3"
                     /protein_id="HEP5640746.1"
                     /translation="MSLTEEIKKRRTFAIISHPDAGKTTITEQLLYFGGEIREAGTVK
                     GKKSGTFAKSDWMDIEKQRGISVTSSVMQFDYAGKRVNILDTPGHEDFSEDTYRTLMA
                     VDAAVMVVDSAKGIEAQTKKLFEVVKHRNIPVFTFINKLDRDGREPLELLEELEEVLG
                     IASYPMNWPIGMGRAFEGLYDLHNKRLELYKGDERFASIEDGDQLFANNPFYEQVKED
                     IELLQEAGNVFSEQAILDGDLTPVFFGSALTNFGVQTFLDTFLEFAPEPHGHKTTEGN
                     VVDPLAKDFSGFVFKIQANMDPKHRDRIAFVRIVSGEFERGMGVNLTRTGKGAKLSNV
                     TQFMAESRENVTNAVAGDIIGVYDTGTYQVGDTLTVGKNKFEFEPLPTFTPEIFMKVS
                     PKNVMKQKSFHKGIEQLVQEGAIQLYKNYQTGEYMLGAVGQLQFEVFKHRMEGEYNAE
                     VVMTPMGKKTVRWISEDDLDQRMSSSRNILAKDRFDQPVFLFENDFALRWFADKYPDV
                     TLEEKM"
     gene            complement(21347..22042)
                     /locus_tag="VC122_000807"
     CDS             complement(21347..22042)
                     /locus_tag="VC122_000807"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010922440.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR02206 family membrane protein"
                     /protein_id="HEP5640747.1"
                     /translation="MDFFAIDPIGLPHTSLIFYLSSLLIALLLVFLTFQAYRLKSYRY
                     FFLFLQLSQVIGLYTWYVLRGFPLDEALPLYHCRIAMLAIFFLPDRNKFKQLFMVLGI
                     GGTFLALLSPDLYPFRLWHVANVSFYLGHYALLVNGLIYLLRFYDASQLRLLSVVRYL
                     ATVNFLLLLVSLATKGNYGFVMDIPVIHTRHLLLNFVIVTSGLTFMVKITEYFYLKFG
                     EAQQLAFAFSKEK"
     gene            complement(22122..23513)
                     /locus_tag="VC122_000808"
     CDS             complement(22122..23513)
                     /locus_tag="VC122_000808"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011184722.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramoyl-tripeptide--D-alanyl-D-
                     alanine ligase"
                     /protein_id="HEP5640748.1"
                     /translation="MKLTLHEVAKIVDAQNNVSDLDDVPLHHIEFDSRKITKGDLFLP
                     LKGQRDGHEFIDLAFQNGAVATFSEKELPGKPHLLVEDCLKAFQKLAHYYIDKMRVDV
                     IAVTGSNGKTSTKDMIGAVLSTTYKTYKTQGNYNNEIGLPYTVLHMPDDTEKIVLEMG
                     QDHMGDIRLLSEIARPRIAVLTLVGEAHLEYFGSRDKIAQGKMQIVDGMNSDGILIAP
                     GDPIIDPYLPENQMVIRFGNQQEIDVTGIQEDKDSLTFTTNVLATPVSLPLPGKYNAT
                     NAMVAAYVGKLLAVTDEDIIAALQTVTLTGNRTEWKKAANGADILSDVYNANPTAMRL
                     ILETFANIAKNPGGKKIAVLADMKELGKDSVILHSQLIDSLTSGNIDQLVFYGDHIKE
                     LARLASQVYPAEQVHYFLKTEQEDQFEAMAQYVQNILNPFDQILLKGSHSMSLEKLVD
                     RLVTDKNCDSYQP"
     gene            complement(23712..24758)
                     /locus_tag="VC122_000809"
     CDS             complement(23712..24758)
                     /locus_tag="VC122_000809"
                     /EC_number="6.3.2.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012678271.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanine--D-alanine ligase"
                     /protein_id="HEP5640749.1"
                     /translation="MSKQTLVLLYGGRSAEREVSVLSAESVMRAVNYDKFLVKTYFIT
                     QMGQFIKTQQFSEKPSESERLMTNETIELTQKIKPSDIYEEGAVVFPVLHGPMGEDGS
                     IQGFLEVLRMPYIGTNVMSSSIAMDKITTKRVLESIGIPQVAYTVYIDGQDLEACLVE
                     TLARLTFPIFVKPANMGSSVGISKAQTKVELRKAIQLALTYDSRVLIEQGVVAREIEV
                     GLLGNDKVKSTLPGEVIKDVDFYDYQAKYVDNKITMAIPADVDQSIVTEMRSYAEVAF
                     KALGGCGLSRCDFFLTQDGQVYLNELNTMPGFTQWSMYPLLWENMGLAYPDLIEELVT
                     LAQEMFDQRESHLI"
     gene            complement(24859..25455)
                     /gene="recR"
                     /locus_tag="VC122_000810"
     CDS             complement(24859..25455)
                     /gene="recR"
                     /locus_tag="VC122_000810"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002896112.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombination protein RecR"
                     /protein_id="HEP5640750.1"
                     /translation="MLYPTPIAKLIDSYSKLPGIGIKTATRLAFYTIGMSNEDVNDFA
                     KNLLAAKRELTYCSICGNLTDDDPCHICTDTSRDQTTILVVEDAKDVSAMEKIQEYHG
                     YYHVLHGLISPMNGVGPDDINLKSLITRLMDGKVSEVIVATNATADGEATSMYISRVL
                     KPAGIKVTRLARGLAVGSDIEYADEVTLLRAIENRTEL"
     gene            complement(26253..27032)
                     /locus_tag="VC122_000811"
     CDS             complement(26253..27032)
                     /locus_tag="VC122_000811"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002994371.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate/nitrite transporter family protein"
                     /protein_id="HEP5640751.1"
                     /translation="MKTPEQILEATIHIGEHKVTKTFLAKSILGFIGGAMISLGYLLY
                     VRIAASGLETFGAFSSIVGACAFPIGLIIILMAGGELITGNMMAVSAALLAKKIKFSE
                     LAKNWLIITLFNVIGAVFVAFVFGHFLGLTSAGIFKEEVIEVAHAKIAASPLQALVSG
                     IGCNWFVGLALWLCYGANDAAGKFLGTWFPVMTFVALGFQHSVANAFVIPAAIFEGGA
                     TWLDFATNFIFVYSGNIIGGAIFVSFLYFKVYYHPQKSKTQ"
     gene            complement(27156..27698)
                     /locus_tag="VC122_000812"
     CDS             complement(27156..27698)
                     /locus_tag="VC122_000812"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002994373.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEP5640752.1"
                     /translation="MTKKPTQLIAYTSILVAFAILIPIIMPLKLIIGPASFTLASHVP
                     LFLAIFMSIPVAILVALGTTLGFLLAGLPLIIVLRALSHLLFAILAAWWLSRKPQLMT
                     SAVKCFSFAFFINVIHGLAEFLVVYILTATTATSMSYFWSMLGLIGLGSLIHGILDFY
                     LALVLWRFLAKNLKLPTINA"
BASE COUNT         8741 a         5774 c         4909 g         8408 t
ORIGIN      
        1 aataaaaaca cgttttatac gaacgttatc tctaaaacaa tggaatggcg ttaatctgaa
       61 gcgccttact caactattgg ttttatttaa gtgagtgacc acacaataaa aaagacaaaa
      121 aagaaaccgt caaactaagg tcaatgaagc ctgcttttca ggactgataa tccactaaaa
      181 tataaaaaga aaatgaccga tagaaatgga gattcacacc aactgtgaat ctccatttct
      241 atcaatccta aactcttttt gtccttgact tttgtgattt tagcttttgt gtatgccttt
      301 ggacacgagt acttcaagtt ctgttagacg ttcgtcaaag actttaaagg cagcttccaa
      361 ataatcacct tttgccatat caacgcctgc tttagcaatc acttcaagtg ggtaatcaga
      421 atttcctgac ttgaggtagg ccaagtaatg atcgatatca tcttgtgtgc catgcacaat
      481 cttatccgct aaatagcttg cagctgcaaa ccctgttgca tattggtaaa cataataatt
      541 gtaatagaaa tgaggaatac gtgcccattc atactgaata aaatgattat cttttttact
      601 taaaccataa tatttttcat ttaaatcagc gtatagctgg ttaaggtatt cacttgttaa
      661 cacttctcct ttttgatcag cttgatgaat ggcatgttca aactcagcaa attgggtttg
      721 acggaaaact gttccacgga aaccatcgag gtaatggttt aaaatggcaa agcgttcttt
      781 ctcatcttgc acttcattta agagagcttc tgtcatgatg ttttcattcg ttgtggatgc
      841 aatttctgcc aagaagatac tataatcccc atacacataa ggctgtgttt cacgagtgaa
      901 agtagaatgc aaactgtggc ctgtttcgtg gacaagtgtg taaagattat caagtgtatc
      961 ttgccagttc aacagcatga aggcattggt atcataggaa ccacctgaat aagccccaga
     1021 gcgcttccct ttattgacgt gaacatcaat ccaacgttcg gtaaaagcac gatgtacacg
     1081 atccgcgtaa tccttaccaa aaacagccaa aaccttctca gccttttcca aagcctcatc
     1141 ataaccaatg gctaaatctg tttcagataa tggtgtgtaa acatcataca tcttcaaatc
     1201 gtctaaacca agaacctctt gacgcaattt caaataacgg tgtaagagtg gcaaatgttt
     1261 attaaccgtt tctaacaagg tatcgtaaac agcttctgga ataaagttag cagccatggc
     1321 agcttgacgt gctgaatcgt atttatgaac acgcgcctta tagttttgca ctttcacatt
     1381 ggtttgcaag gttttagcgt aagtatgttg gaactgttca taagtactat acatggcttc
     1441 ataagcagct tggcgaatgc tacggtcctt agattccatt aggctaatga agttaccatg
     1501 ggttaattca acatcttccc ccttatcatt tttaactact ggaaagacaa tatcagcgtt
     1561 atcaagaata ctaaaggttt cttcagcgcc gttgaaaatt tcttgggctc ctgctaataa
     1621 ttcctcttca gcctggctga gcacatggtc acgtgtctgg aacaatttgt caaagaaatg
     1681 atgatacctt tttaaatcag gtgtttccgc aagaaaagct tggtaatctt cctgactcaa
     1741 tgccataact tctgggtcat agaatgaaaa cacttcgcta aactttgcat acaagcctga
     1801 agccttagct tggtattctt gatacttggc aacagtagtg tcttggtcat ttttcatgtg
     1861 agcataaaca tagactttct caacacgacg agctaactct aaataagtat ctgtaattgt
     1921 caacagatta gcgctagaat ctagtaaatg acctgaaaaa tccttagccg cgtcaatttc
     1981 agtagctaag tcactgactt cagcttccca atccttatct gttgcaaaaa tagtgctcaa
     2041 atcccaagtg tatttttctt ctaaatggct gcgattatca gtcataacat ctccttctca
     2101 ttttctatta ctatccattt taacataata ttttggtgaa aaaagcgttt gcaccttctt
     2161 atccttttct tttttataat agactttaaa actttcataa taagggcgaa gatttccttt
     2221 aatttgacaa aaaccgctgg aagattgaac tggtctccat tgaggataaa aagcatcagt
     2281 tgttaacata agtaaattat agcctgataa gtaagccttt tcttgtcgtc gtagccattt
     2341 cgggtgtcta gctaaaagag ctctttgtat tcttttagat aggttcacag tcattttttt
     2401 ctgataagaa taaaggattt cttgctgata gggtaatcga aacatttcca taatattgtg
     2461 gtctaaagaa atggttttgg ttagataact taccttacca aataagtctt catggattaa
     2521 atatttgaga cgtagcagat ttgcagctgc atccagctcc cacaaatgaa agcccaaact
     2581 agatgaaaag taaagaaact gtttttgcaa agcagttaag tgagtgttaa gccacaattt
     2641 tcttcccagt aaccatcgta ccggataccc cttttcttga taggctttgg tgcgtttctt
     2701 tagacgctcc actggcaagg gactgcactg aatttctaag gctaacttat cattcaccca
     2761 aaggtctgct atctgttgca gctcaggaag atatttttca atacagacag cttctgttcg
     2821 gactaaactg gtgtaaagtt ttgccttcaa cgtcaaatgt tcctcagatt cattttctgc
     2881 ttggaattga caatgagcta gttgtacatg ggcaaaatga ggtcgtctaa ttgtcccctg
     2941 acgcaagcga acaggagatt tgcaagctgg gcaacaaaaa ggcggtttcg ttgaaatagg
     3001 ctgagttact agcgaaatga gctgattctt accatctaaa gcggttaata tttttgtcac
     3061 ctcctattag atcttccacc ttattattcg aaaactaagt accaaacttt aagtagcttt
     3121 agttttttta gccatcaaaa aacactccca tttccaattt atgagagttt aacacaaggg
     3181 tttttataac gcttcaggtt aaggagacag tgggaattga atttttttgc ttacctgatt
     3241 ttgtaacaac aagatgacta aacatagcaa aagtcggact catcatgact ataatagtca
     3301 atggacactt tattgattaa gcaagaatag cttctttttg aaagcatgtt ttgctcagtc
     3361 tagatacgtt ttaatgagtt gcctttctga cgctgttaga taacgatatt ctccgctggc
     3421 taaatgaccg agttccaatc ctgcaaagtg cgtccgtttc agagaggtca ctttcaaacc
     3481 ataggtcaaa aacatttttt tcacttggtg aaattttcct tctgaaatgg tcaaactagc
     3541 ctgtgactgg tctgtgtcgg ctttaagaat agtcaattga gctggttgac attgttcacc
     3601 tgttgggaaa caaataccag ctgcaaaaaa atctgaggca tcttctgcta acaagccatt
     3661 aacggttaca agatacgttt tagaaacatg atgaataggg tgcaacatcc gaaaccctag
     3721 gggaccatta tcagtgagta aaaccaaccc ttcggtatct ctatccaatc tgccaacagg
     3781 atacaagtct ggacttttat cttcttcagc aagctgatca ataacggtta aatagttagt
     3841 atctttctta gctgagacca cccctgatgg cttgttgagg ataatgtagg aatgtttagg
     3901 atgcgtcact cgctgtccag tcacctcaat cagttgcaag ccaggatcaa cattttgtcg
     3961 accatttctg gcaggcatgt gatcaaccca aaccccttga gccttaatca gtttcttcac
     4021 ttgacttcta gagccaactt ttgtaccttc taataattta tctaaacgca tattagtagt
     4081 gtaacataaa tttacccttg caaaagaaat agaagttaca agacttcttc ttaaatgagg
     4141 ttgactatct cctcattttt gttcttacaa ggtttaacga ctggttaggt taatctaatt
     4201 gtgacgcagc agcttcatcc acaatgacga taacatgatc atgtttttgg agaatacttg
     4261 ctgggaggtc ttctgtgatt ggtccaaaaa ccattccctt gatcgcatca gctttttctt
     4321 gtccaaaggc aagaagaaca atcattttgg acttcatgat ggaggcaatt cccatagaga
     4381 tagcttgttt tggcacatcc tcaatacttg taaagaaacg actattggct ttgatagttg
     4441 attcctgaag atccactaca tgcgtttctt cttcaaaaga ggtcccaggt tcgttaaaac
     4501 caatgtgccc attacgccca atcccaagca cttgaaaatc aatcggatgc tctgcaatga
     4561 tttggtcata tcgtttagct tcagcttcga tatcagttgc taaaccattg ggcaagtaat
     4621 tctttttaaa aggcttcgca ttaaaaaggt tttgacgcat aaaataatca taactctgat
     4681 cgctctctac tgaaagaccg acatattcat ccaagttaat acttgttaag tccgaaaaat
     4741 ccaaaggact tttgaccatt tcttggtaaa aactaattgg tgaacttcct gttgctaatc
     4801 ctaacgtctt agctccctta gctaagctat ctttcaataa ggtaaaagcg attttcccac
     4861 cttcaatctg atcttggacc cgaataattt tcatgtcatt ctccttttct aattggtata
     4921 gacctttcgt gtttttatta tatggtatat accaattttt gtcaagccta tcctttactt
     4981 tctgatgaaa gattaaagat gacgggttag acgacaagtg atataataga aaagatttat
     5041 cttacatgaa agagttacta ggaagagatt tactagtggc tttaaaggag tcccaatgaa
     5101 tactaacgat tttgattttg agttaccaga agaattgatt gcccaaaccc cactcgaaaa
     5161 aagagacagt tcaaaactat tgattattga tcaccgtcaa aaaactatgg ttgatagcca
     5221 ttttgaccat atcattgatc aattaaatcc aggggatgcc ctcgtcatga ataacacccg
     5281 tgtcttgcct gccagacttt atggagaaaa acccgatact catggtcatg tggaattgct
     5341 tttgcttaaa aatacacaag gagatcaatg ggaagtttta gccaagccag ccaaacggct
     5401 caaggttggt agccaagtca attttggaga tgggcgtctc aaagccacca ttatcgacga
     5461 attagaacat ggtggacgaa ttgttgaatt cagctatgat gggattttct tagaagtttt
     5521 agagagcctc ggcgaaatgc cattgcctcc ttatattcac gaaaaattag aggatgctga
     5581 gcgataccaa accgtttatg ctaaagaaaa tggctctgcg gccgccccaa ctgctggtct
     5641 ccacttcaca acagacttgc tcaaaaaaat cgaagccaaa ggggtacatt tggtttattt
     5701 gaccctacat gtagggcttg ggacttttag acctgtttcc gtggataacc tcgatgagca
     5761 tgatatgcat tcggaatttt attccctctc agaagaagca gcgcaaaccc taagagacgt
     5821 caaacaagct ggcggtcgtg ttgtcgctgt aggaaccacc tctattcgta cgttggaaac
     5881 catcggaggt aagttccaag gggacattca agctgattca ggttggacca atatttttat
     5941 taaaccaggc taccaattca aggtagttga tgctttttca actaatttcc atcttccaaa
     6001 atcaaccctt gtgatgctag tatcagcctt tgcagggcgt gactttgtcc ttgaagctta
     6061 cagacacgcg gttgacgaaa agtatcgttt ctttagcttt ggcgatgcca tgtttgtcaa
     6121 ctaaaaacct atgaaattac ttggaaaatg gctattcttc ttgatgtttc tgactttttg
     6181 cgtcagtttt tggcagaaag actatcagcg gattgccttt gaaaaagaac acaaacttta
     6241 tcaaaattta gtggcacctt tttaccaaaa ctatcctaaa ttacgagaac tccagctttc
     6301 agaagctatt aaattacgcc acgacttgct tgattatgtt cgaaagctcg ataaggacgg
     6361 atggtcctat aaagccatcc aaaaaggata ccttgaacaa ctcgctgtta gaggaaactc
     6421 ttatcatttc gaacaacttt atagtcgcat tcgcctgata ggatcacctg actttcaaaa
     6481 gctttggcaa caagaagaga tggcacagac gcctcaagaa gctcaaaagc ggttacaact
     6541 gctcttgacg tatttaaaga tgccagacga gttgacagga caatcaaaac aaacacagca
     6601 agtattggca caattaagtc caaagctatc gccaacagat cctttttggg accaattatc
     6661 taccctgatt caggcttatt atgttaatct cgaacacatt ccttatagcc gttttaatcg
     6721 gaaaattcat caactccgtt acctcatatc aacccagcaa acggagtggg ttcgctcaca
     6781 ttatgggaat aaagggaaaa ccgatgcgga tgccttagcc aaatatttgg ctactctaga
     6841 agatgacgat tatgctcttt atgaatcttc tagataccat aataaggttg ctagtatttt
     6901 agatgctaat ggcaatcatc aggcagtcta tacagacaat attcctcaag ccaactataa
     6961 gattttaatt cattttcact cagagtttat tttgtcagaa tctggacaat ttttagtagc
     7021 actagatcct gataacctaa cgcgaaatag tattgtcaat ggttcttctt tcaattatgg
     7081 caaccaaaac gatgacttgc accgtctgct tgatattgac cctattctgc tttttgatcc
     7141 ggcttttatt gaaaaagcaa ttaatagtcc tgatgcaact tttctagttc ccgatttaga
     7201 acagcaaagg gataagcaca atcccattta cagcagaaat ggcaaatcat ctaagcagct
     7261 aacacgcgct gccgttaaaa aattcaaaaa attgcttcac cattatcaag aaacaagagg
     7321 tgacaataag acctcaaaaa cacagcatta atctcaaaaa gcttgacctg aagaaacagg
     7381 ccaagctttt ttaatgagca aaaacttatt ttagtgactt tcatcagtaa gtatgtctac
     7441 tatcagacta atgtcaactg agtattagat catgttgtga ttgactctat gccactcatt
     7501 aattacatat gttagctgaa caagctgcac ataaccaagg ccattgactt cctctgatgc
     7561 ttgtgttgct ccacctaaac cagatgaata aagagtaatc aaataaggag actcttcgca
     7621 aacaatggca cctacattta aggcttctct gacataaccc ggtttttgat aaacctttac
     7681 atgaggcaaa taagtcttgt aatacaaatc agggaaagat tcaccaatgt agtacaagat
     7741 atccttatat ttatcctgat gctgccacaa gtaatccaaa ccttgcaaat agtaagcggt
     7801 ggtgatttta ttgccattac gatcaatggt cgtaatagct ccggatacct tcccgtagcg
     7861 gtcaaataat tggtaagcct tattcatgcc acccaatcgt tctgctagag cataagctgg
     7921 cgtgttttct gagtacacca aggaatattc ttgcatatcc ggaatagaca tggcaccatt
     7981 gaattgagca acataggcat gatgttcctg atcatattca taatcagttt ctgtaatgtc
     8041 aaaacgctct gtcatggata atttgcctta tctaccacca gcatgtttaa tggcagctta
     8101 taggtgcttc ctgcaatcat tggctgatgg tcattcatgg cagcagtcct gcctgaattg
     8161 aggtctttgt aggaaaaggc aatttgagat tcaccaatgc caaattcctt taaatacacc
     8221 ttgaccgtat tgaccaaact caaatggttg tcatcgtaat agagccctaa ggcatccatt
     8281 tgcgctaaat ctgcatccat aacagcttgc ttagcctctt tagatttttc aggtaacggt
     8341 ttttgaacag tttttctaat cttttttcat ttttaagtca gccgtttgct gccgctcacc
     8401 tgctttagga ggaaagcctt tctttttacc agtcaaacag ccgacaagta aggtcactat
     8461 catcattcct aaaataatcc ctacaatggt agttttcttc tttcttaaca cctaaaatct
     8521 cctatccatt ctattctagc taaatttagc taaaaatcaa ggaattacat taaaaagaca
     8581 aactattttt atcttttcta aaaaatgatg tccccacact gttattcaca aaaccactta
     8641 aaaaaccaat ctctttttga gattggaaac ccttttctta tttagcggct tggtaaagtt
     8701 ctgatacttt tttccaatta atgatttcaa agaaagcttt gatataattt ggacgtacat
     8761 tacgataatt caagtagtaa gcatgctccc acacatcaag cgctagaatt ggttttttgc
     8821 cttctgaaat tggagtatct tggttagcgg ttgatgtgat ttcgagttgt ccttctttat
     8881 taacaactaa ccatgcccaa cctgaaccaa aacgtccagt agcagctgct gtaaattgtt
     8941 ctttaaaggc gtcaaatgac ccaaaagcat catcaatggc ttgtgcaaca tcaggagtga
     9001 cgtcttgttt ttcaggtgat aatagttccc agaaaagcgc atggtttagg tgtccgccac
     9061 cattattaat taaggcttga cgaatatctt ctggaatctt agtgacatct gccaatagtt
     9121 cctccagatt ttctccgatc tctgggtgtt tttctaaagc cgcattggta ttagcgacat
     9181 aagtggcatg gtgtttatca tgatgaagag tcattgtctc cgcatcaaac tgtggttcaa
     9241 gagcatcata cgcgtatgga agttctggta aaataatagc cattttcctg tcctcttttc
     9301 ctttatgata cgtctatctt atcgcaaaaa ataagagctt tcaactaatt tgattatctc
     9361 ttggaaagcg gtccaacgcc tatttttgag agtgagtcat gatttttaag agagcaatat
     9421 caactagata actcttctca taaagtcctg tttttatctg gtaatctgtc tcaatcaagg
     9481 ttttcaccgc tcctgttaga aaggcaagag ataaggtcct agaatccttt aacgcatact
     9541 tgacctggta aggattaacc cgccgatcaa gtatctctga caaactggtt acaagctgct
     9601 gttctgattt tccttcttta gcaagaatgg caagctgtaa atataagcga aattgcccca
     9661 acataatagc aattaattta atatcatctt cccctgataa gcgtaaatca tgaatcaaat
     9721 ctctagccac atcaacttta cctcctagga caagtctggt taagtcaaaa atattgtctt
     9781 gtaaactttt aggaatggct tgctcaatat cagttaggct aatatttccc gtttttttat
     9841 aggcttttaa aaaagccatg tttttcatga tttgactaaa atcatcgttt gattttaaaa
     9901 gcagttgttc aaaagcgcca ctctcaaatc ccaaacccag ttgatgactg tatttttgaa
     9961 aataagttct tagctctgct tctttcagag ggttggcttc taaaacaagg gcatcacgtt
    10021 tcaaaagctt aacaagccgt ctcttactat ccaatttacc tggagcaaag ataattagtc
    10081 gagtagtctc taagggattt tctaaatagg cttcaaaggc ctttaggtct ttttctttta
    10141 agaaactttt tttattggtc gtgatatcta acaaatggtc aaaaatgacc accttctgct
    10201 cagcaaagaa gggtaggctc actagatcca tttctgcatc ctgataagcg gcctcagaca
    10261 tatcaaagta agaataggcc aaatcatcct tatcaaaagc aatctgctcc attaagcggg
    10321 atttcaactg gctatactga ccaatgtcat ctcctgtgac aagggttata agacccaaat
    10381 tttctttact cagtttttca atcttttcta tcgcaatcat gcttttatta tatcatcttt
    10441 ctcctaaaag gaaagcgact gcttgtttct tcgtcaggct ggaattttaa ctaacggacc
    10501 gtttcaagat gccagcttgt ccaaccagtc aaacgaatag ctccttgcgt atccgttcgg
    10561 taataagtaa tttgtctgtc ttccaaacgg gctaaggttt ctctgtgagg atgctgataa
    10621 cggttatttt ttccagcaga aataaaggcc actttaggct gaatatggtc taaaaaagct
    10681 gcactagaag aggtatttga tccatggtga cctgctttta aataatctac tcttagttga
    10741 ggataacgtt ttatgatctc gttttccccc tctttttcca agtcacctgt gaaaagaaaa
    10801 gttcgattta agagcctgcc ataaagtacc aaagaatcgt tatttttccc gtcccctaac
    10861 tgccaagggt agagtacctg taagacactt cccataatag gcaactggtc tccagctgct
    10921 aaaaccctaa cgtgacattt taaacgcctt agccgcctaa caaagcttgg atggcttaaa
    10981 ctgccttgac tagtcaaaat ctcctttatt cgaatagctt tggcaaccac ttcgatatcc
    11041 cccatatgat cagtatctgc gtgagttact aatagttggt caattgtgtg aatacctcta
    11101 ctttttaagt aaggaattaa ggttttctga gcattagcta cgtgatgtcc tcgtcgccag
    11161 tgctcttttg aagaaaagaa agggcgccca cctacatcta ttaagagatt tttccctgtc
    11221 cactccctca ccaaaatact atctccctgg ccaatgtcga taaaggtcac ttcattggtc
    11281 aaaggatatt tgacagacaa aagcgtcaac gctataacca aaccgcaagt gataactcgt
    11341 tgtcgcacct gccgataatc ataaaaaata gcgaagctaa tcaccaagat aagcaagtgc
    11401 caactggtcg ggcttccaaa aacaagggaa ctattgaaag tatttccgag aaattgaatg
    11461 actttttcta ataaaataaa aagatggtta catattgaga cagtcaccaa aggggataga
    11521 caaaaaacca gacaaagaag gggcaaaata aaaaggtcaa agagataaga caagagccct
    11581 gttaagacca ttgacatggg gttaaaacta gaaaaataaa agagcaaaaa tggtaatatt
    11641 cctagggaaa tggtcaagac ttgtaccaac tgtcttttag ctcctgataa ttcttcaaca
    11701 gtaaccactg ttaacaaaaa agcatagcta aacgttaata cgccaccaac agtcattaag
    11761 aaatgagcat cccataaaaa gacaagtaaa aaagtacaag ccagattgtc taatcctttt
    11821 attcctaaat gacgcaactg cgactgcacg aggcttcgaa taacagaaat actataacct
    11881 gtcaacgcgg cataaaagca agcaaaagga agctctatcc attttatcca ttccaaagga
    11941 actgcgagta atagcaacac ccttctaaaa caagtaagaa agaagccaac ctgcatccct
    12001 gataaagcaa agagatgaat gatacccaac tgactataat aatctgtcat ctcaccaaac
    12061 gacttatcta aataaccaaa aaggaggccc gtcaggtagt gagacatagg tcttggaaaa
    12121 tgctgctggc aatgaacaat ggctcgtcgt cttaaagagg acaaatagtc acatattgac
    12181 tcgggtgaaa tcacttcaag ctgttctatt tgctctacct tccctatcct gtaaattcct
    12241 tgataagcca aaaaagtctg ataattaaag cctttaaaat ttcgaacttc ctcagctttt
    12301 tccaatgtca cttttgcgtg catcactaac caacggtgct ctttcttgaa caattgggct
    12361 tctgcttggc ttttcaaacg gtaaaataac tgataagaat gtttcccgtg acgtccaaga
    12421 acagtaattg atcaccatta atccgaatac tatcaggaac taatgctaca gaggttattt
    12481 gtactggctg aatttccagt ttttgaatca actgctgttt ctgatacagc aaaaacaaag
    12541 cacagaaact aaggatggca aagacaccga gaaattcttt tttagacaaa cgcttgacaa
    12601 gtaagcaaat caaactaagg ctaaggagaa acgttaacca gctagggcta tggatttgat
    12661 aaaagagaac caagattaaa aaagcaaatt gaatctttga caggggcact agcttagtcc
    12721 aagaaaatgt catcttttaa tttctcgagc gttttttcgc caatccccga aacctgacgg
    12781 agatcttcta acgctttaaa accacctaac ttatctctca tatcaataat atcctgagct
    12841 cttttagcac caatacctgg gatatgttgt agttcttcta agctggcctt attaatatga
    12901 acctttgact gatccttgct ggcagtttct tttttaccag aaactaatga tcttggtagc
    12961 acagaaatct cttctccttg tttaggtaca taaacaactt gctcatcagt tagtttttct
    13021 gcaaaattaa tagcatgttt atcagcttct gaagttaggc ctccagctag ttcaatgaca
    13081 tctcgcacac gactgctagc agttagctta taaactccct ctttttgtac ggcgcctttt
    13141 aaatccacta aaatactgct atcttcttga atttcctttt tttcttcact aacctgctgt
    13201 ttcacaggcg atatttctct caaagcagga acggctgcct tgctctgcct atttccccag
    13261 aacaaaaaac tgaagcttag tattagaaaa agtcctaata aaagactaat taggtatggc
    13321 agaaaatacc gctctctcaa aaacgatttt cctttaatta ataattcgtc taacattttt
    13381 ttacctcatc tttttattcg taaatgatat ccaaaagtat agtagtattg tcattttttg
    13441 tgcttaaaag agttaagggt atcatcgaat atctttttta gaaatgaaaa aagtgattaa
    13501 gcattagcgg cttctttcaa tagaagacta atctcaatca cttcttattt caggcacatg
    13561 ttagccctca tgttcttaat ggcgatgttt atctggattc caaacaaaac tagcaatata
    13621 ggaaaataat atggttaata ataataccac caccaaaacc aatcctaatg gtaaacggta
    13681 gagataagtt aaagggttcc ttgcttttcc tggttggaaa ggtgccaact cgtcatccat
    13741 ccgatcaaac tctgcctgaa tacggtttgc cacctcagca atcccttcat cattcatgcg
    13801 tttaatatct gagacgtcaa tgggattccc aaaagtcatg tcaacccgtt ctcccgctag
    13861 taatcccttg accgacatag gcccctgata ggctgctggc ataatcttga ccttagccaa
    13921 tttagcaatc acagcgaccc ctcctttgac ctcttgcgaa tgcctacttc cacttggaaa
    13981 cataagaaga gaacgattgc tctttttgag catattaaca gggtaacgaa tagcatctgg
    14041 actcggtttg tcacgatcaa taggaaaagc accacacatt ttaatccacc aagcaaagag
    14101 acgatttgca aacaactctt ttttagccat aaaaataaac tgcttaggac gtgctgcaaa
    14161 tgccatgtaa actggatccc aaaaggttcg atggggtgct accagaatat aattctcaga
    14221 agcatctaac atcttttctt catgatggta gtgggcattg ccattgacaa cccataataa
    14281 aaagactact aaaccgcgta agtaagcgta aaacacaggc ttctccttta atagaataat
    14341 cttacttatt gtatcatgaa agctgacttt tcgaaagtca aaatcactct tcaaacattc
    14401 acctcaagcc tattttatgt taaaataaca ggtatgataa aagcaatctt aaaagaaggc
    14461 gaacgtattg atcaactctt ttcaagtgat gttggcatta ttcaaaacaa ggatgtcttt
    14521 agttactcta ttgatagcgt cctcttatcc cgcttcccca agatgccttc caaagggtta
    14581 attgtggatt tgtgctcagg aaatggggcg gttggcttat ttgctagcac tagaaccaag
    14641 gcagccatcg ttgaagtaga attacaagaa cggcttgctg atatgggaca acgctctatc
    14701 cagctcaatc aactggaaga ccaagtaacc atgatttgtg atgatttaaa aaacctccta
    14761 aaccatgtgc cacgttcagg agttgatttg atgctctgca atccccctta ctttaaaagt
    14821 cacgaaagtt ctaagaagaa cgtctctgaa cattatttac tggctcgtca tgaagtgacc
    14881 actaatttag aagagatttg tcaggtagcc cgtcatgccc ttaaatcaaa tggtcgtctg
    14941 gcaatggttc atcgaccaga tcgattcttg gaaattatag atagccttag agctaatggt
    15001 cttgccccta aaagggtaca atttgtctat cctaaattag gtaaatcagc taatatgtta
    15061 cttattgaag caatcaaaga cggttctatt gaagggatga ctattttacc tcctttagtt
    15121 gtccacaagg aaaacggaga atacactgat catatttttg agatttattt tggtgctgct
    15181 tccaaaggaa aacctaatca tgaccactaa aaaagcttat atgtatgtgt tagaatgtgc
    15241 agataaaacc ctctacactg gttatacaac tgatttgaaa aagcgtttag ctacacataa
    15301 cgcaggaaag ggggctaaat atactcgcta ccggttacct gtttctttac tttattacga
    15361 agtgtttgat agcaaagagg ctgcgatgag cgctgaagca ctctttaaaa aacgaaaaac
    15421 aagaagccag aaattggcct atattgctac tcatcaaaaa gaaaagaaaa atcattgaga
    15481 cttatcatct caatgatttt tttatgcttc actgtcttcc acaggcacca attctttgat
    15541 tggtaacttc aagacatctg atagaatcaa gggaaccact tcaagggtta cttcggaata
    15601 ttgaagccca aaccaacgca ttggactagc ggtaacatgt ttaatgcttg ccttagtctg
    15661 cataataaag cgttcaaagt tactcaattg acgtgcattg gtgacccgtt cttctattaa
    15721 aacaaagcga aaatcaccaa catcacgacc aggtgtgatg gtatattcct gttcttgttt
    15781 gggtaaacgg ccactttcca tcaaatcctg aacaatcgtg cgcaaatagc gtggcacagt
    15841 ctgtggcatt ctaaaaccaa gataaaggtt gactcgaacc atataatccg ttcccatcat
    15901 atctaccttg tacttagctg tataaggttc atcagttacc tggacattta caaaccaata
    15961 aacttgagcg cgttttggcc gcttgtctaa aatggagtac aaaatagacc ggtcaatcat
    16021 atgatcctgc atacgattgg ataagtagac aacatttgtt tggtagaggt caaaacaaac
    16081 gtcgtcacga agttgtttaa tctgctcctt gtaatcattc aaattaagag atttcacata
    16141 cttgaaaaca atccgtgtgc ccgcatgcca gataaacatg acaaagacaa ttgcaagtgc
    16201 caagataaca accgcatagc caccgtgcat aaatttgata gctgaagcca agaagaaaat
    16261 gaattctaca agggcaaaga aagccatcgc taaatgagcc aagatgggtc tcgttccttt
    16321 cttgattaag taatacttta agaggattgt tgtcatcaac atggtgatgg taatagcaag
    16381 accataagct gcctccatgt gggctgatgt tctaaaagcc aaaacagtac aagaggtcac
    16441 tgcaaataaa atccagttaa tgacaggaat atacaattgc cccaaatttg cccctgggta
    16501 agtcacacga aacaaaggga aaattttcaa acgcatggcc tctgaaatca aggtaaagga
    16561 gcctgaaata agagcctgtg aggcaataat agctgccaag gttgccaaac taacaaggta
    16621 aactcttaat tgtgatggca cactagcaaa gaaagggttt agttcaatac ctgagtgttt
    16681 attcgctaaa atccacgcag cctggccaca gtaagacaac acaatacaca tcttaacaaa
    16741 gggccagctc acataaatat ttcctcgacc aacatgtcct aaatccgaat aaagggcttc
    16801 agctcctgtt gtcgcaagaa aaatggaccc caagataaaa atcccacgat ggttttcagg
    16861 actaaacagc aaatgcagag catagtaagg attgatagcc ttgaaaattt ccagatgccc
    16921 caacgtatta aaaaaaccac taacacctag aaaactaaac cagataaaca taactggtcc
    16981 aaaaatttta ccaataaaac ctgttccaaa ccgctgaata ccaaacaaga ctattaaaat
    17041 cactagagtt gtgataatga cattggtttg attttggtag atgtgactca gaccaggaac
    17101 ggctttcaag ccctcaatag ctgaagtaac tgttaccgca ggtgtaagag ccccatcaga
    17161 caaaagtgtt gcacctccaa tcatcgccgg aataatgagc cacggtgata ttttacgtac
    17221 caaggtgaat aaagaaaaaa tcccaccttc atggtggtta tcagctttta gagcaatcaa
    17281 aacgtacttg attgttgtga tcaaggtcag tgtccaaata attaaagaga ttgatcctaa
    17341 aataaaggat tcagaaacct gattaacccc tccttgattt tcaactagag actgcatcgt
    17401 ataaaggggg cttgttccaa tatctccata aacaatacct aaagcaataa taaaacctgc
    17461 ttttgaagct ttgtcaaagg cagtaagatg gctatcagac atcttatact cctcctaatt
    17521 ttttaaaaat tataatgaaa taactgaaac cattataaca gatcccatta taaaagcaac
    17581 tttttcagaa aattaaaaag agctgactct catcaactct ctttaagctt attcaccttt
    17641 gtgacgaatc acaaaaccag tatttttagc acttgatttt ttctctttat tgaaaaagtc
    17701 tttactatta cgttttgatt tacgtttgaa atcgcggttg ccactgccat cgtgaccatc
    17761 acgcttatca cgtttttgac gacgacggtc accatcacgc tcatctctgt tacggtcacc
    17821 acgatagcca ccacggcgat caccacggtt acggttgtca cgtccacgac cacctttgcc
    17881 atttttattg ccgtgaccac cacccacata tttgaatggt aatggttttt cacgtgcaat
    17941 ttcaacttcc ggtaagctat ctggatcttg aaccgttaag ctcaaaatat ataaagctaa
    18001 ttcttcagga gtgaattcgg cagccaattg gacagcatcg cctttgaatt tatcaaagtt
    18061 tgaacgaata gtctcatccg caaagtcgcg ttcaattttc ttgagggcta cttttttctt
    18121 ggcttgaaag gcctcttcag cagttgctgg acgaagaggt ttcatctgtt ttttcgttaa
    18181 gttctcaatc attgagaggt agcccatctc atttggtgag acaaaagtga tagactcacc
    18241 agatttaccg gcacgacctg tacgaccaat acggtgaaca taactttctg gatcttgggt
    18301 aatatcgtag ttataaacgt gggtaactcc tgaaatatca aggccacgcg ctgctacatc
    18361 agtggcaaca agaatatcaa tttggtcgtt tttgaaatca cggataacac gtaagcgttt
    18421 attttggtca agatcaccat ggattccttc ggcacggaaa ccacgaagtt tcaagccacg
    18481 tgtaatttca tcaactcggc gcttagtccg cccaaaaacg atggataatt ctggctggtt
    18541 aacatccatc agacgggtca tagtatcaaa tttttcttgt tctttcacac gaacatagta
    18601 ttgatcaaca ttgacgtttg ttaattcttt gttcttaatt tgaacatgtt cagggtcttt
    18661 catgaattta accccgattt gtttaatagg tgccggcatc gttgcggaga agagcaaggt
    18721 ttgacggtct gctgggacac gactaataat agcttcaatg tcttccaaga atcccatgtt
    18781 aagcatttca tctgcttcat caaggattaa ggtttccaca tgatctaaaa taagagcctt
    18841 gcgcttaatc aaatctaaca agcgacccgg tgttcccacg acaatgtggg cacctgattt
    18901 aagggctttg atttgttttt caatacttga tccaccgtaa acagagcgaa ccttaacccc
    18961 tttttcacgt ccaaaacgga acaattcttc ctgactttgt acagccaatt cacgcgttgg
    19021 tgcaatgacc aatgcttgga tgatattttc gttagtacga atcttattca aggttggtag
    19081 accaaacgct gctgttttac cagttcctgt ttgtgcttga ccaataacat ccttcccttc
    19141 tagtgcgaga ggaatcgtca tttcctggat aggagatgct ttctcaaaac cagcggtaac
    19201 aacagctgat tggatgtctt gtgataagtt aaattctgta aatttcaatt gtttctcttt
    19261 tctaaaaagg cagtgcgaag ctgccttata aaccttttaa ttttcgtcgt ttgattaaca
    19321 acctaactag tgtaacacaa ttagaatcaa aaagatagga aataaggcta atataagcaa
    19381 taacacgctt tcacgattat ccgctaaatc ataaatatgc ttacagttgt gaagatatcg
    19441 ttttttgata gaatcttaaa tctgcaaaag agataactag ccactcattt cttttaaaaa
    19501 gttgctcagt aaattgatct ccaaaacgtt agactgttga aaatcatgtt tcgattacat
    19561 tttctcttct aaagtgacat ctgggtattt gtctgcaaac cagcgaaggg caaaatcatt
    19621 ttcaaatagg aaaactggtt ggtcaaaacg atccttagct aaaatgtttc ggcttgagga
    19681 catgcgttga tccaaatcat cttctgaaat ccagcgaaca gtctttttac ccattggtgt
    19741 catcacaact tcagcgttgt attcaccttc catacgatgt ttaaagactt caaactgcag
    19801 ttgacccaca gcacccagca tgtattcgcc tgtctgataa ttcttataaa gctgaattgc
    19861 tccttcttgt actaattgtt caataccttt gtggaaggat ttctgcttca taacattttt
    19921 cggagaaacc ttcatgaaga tttctggggt aaaagttggt aagggttcaa attcaaattt
    19981 atttttccca actgttaagg tatccccaac ttgataagtt cctgtatcat aaaccccgat
    20041 aatatcccca gcaacagcat tcgtcacatt ttcccgtgat tctgccataa attgagtaac
    20101 attggagagc ttagcgcctt tgccagtgcg tgtcaaatta actcccatac cgcgctcaaa
    20161 ttcaccagaa acaatacgca caaaagcaat acggtcacgg tgtttagggt ccatgttagc
    20221 ttgaatttta aagacaaaac ctgaaaaatc tttagctaag gggtcaacaa cattaccttc
    20281 tgtagtcttg tgaccatgag gttctggggc aaactccaag aaagtatcaa gaaaagtctg
    20341 aactccaaag ttcgtaaggg cagagccaaa aaagactgga gttaggtcac catcaagaat
    20401 agcttgttct gaaaagacat tacctgcttc ttgaagaagt tcaatatctt ctttgacttg
    20461 ttcataaaag ggattattgg caaacagctg atcaccgtct tcaatacttg caaaacgctc
    20521 gtctccctta tagagttcta aacgtttatt atgtaaatca tataagccct caaaggcgcg
    20581 tcccatacca atcggccagt tcatcggata agaagcaatc cctaaaactt cttctaattc
    20641 ttccaagagt tctaaaggct cacgcccatc acggtctaac ttgttaataa aggtgaagac
    20701 aggaatatta cgatgtttga caacttcaaa aagttttttc gtttgcgcct caataccttt
    20761 agccgaatct acaaccatga cagccgcatc cacagccatt aaggtacggt atgtatcttc
    20821 cgagaaatcc tcatgtccag gtgtatccaa aatattaaca cgtttacccg cgtaatcaaa
    20881 ttgcataaca gatgaagtaa cagagatacc acgttgcttt tcaatatcca tccagtcgga
    20941 ctttgcaaac gtacctgatt ttttcccttt tactgtccct gcttctctaa tttcaccacc
    21001 aaaatataat agttgctcag taatagtcgt cttaccagca tccgggtgag agatgatggc
    21061 aaaagtacgg cgttttttaa tttcttctgt aagtgacatt cttttttcct tgtatagtaa
    21121 aatggcagag cttttctctg agttattttc gttaggtatt acttgtttat caaaagagtt
    21181 gggctacatt gggtttctcc atatacgatt agcctctaaa gttcttcaaa actggtatta
    21241 taacttatct tgggctatca atgagtatct tttttaaaaa aatcgagcct ggaaatcatc
    21301 ccagactctt cttattatag ctgattttaa gtgctttttc aacgacctac ttttctttag
    21361 agaaagcaaa tgccagctgt tgagcttcac caaatttaag gtaaaaatat tcggttattt
    21421 taaccataaa agtcaagcca cttgtaacga tcacaaaatt aagtagcaaa tgacgcgtat
    21481 gaatcactgg aatatccatc acaaaaccat aatttccttt cgtggctaaa ctgaccaaca
    21541 gaagaagaaa gttaacagtt gctaaatagc gtactactga tagcagtctt aactgactgg
    21601 catcgtaaaa acgcaagagg taaatcaaac cattcactaa caaagcataa tgaccaagat
    21661 aaaacgagac atttgctaca tgccataatc taaacggata aagatctggc gataaaagtg
    21721 caaggaatgt tccacctatc cccaaaacca taaataattg cttaaacttg ttacgatcag
    21781 gtagaaaaaa gatagctaac atagcgatgc gacaatgata caacggcaaa gcttcatcta
    21841 aaggaaaccc ccttaacacg taccatgtgt acaagccaat cacctgagat aactgtaaaa
    21901 acaagaaaaa atatcgataa gattttaacc tatatgcttg aaaagtcaaa aaaacaagca
    21961 aaagcgctat caagagtgac gacaagtaaa agataagtga ggtatgaggc agtccgatgg
    22021 ggtcaattgc aaaaaaatcc ataatcgtcc ctttctatat tttttcatta taccttaatc
    22081 ttcatgagag aacaagactt tatctgaaag ttttataaaa atcaaggctg atagctatca
    22141 caattcttgt cagtcactaa tcgatccact aacttttcta gcgacatcga gtggctgcct
    22201 tttaaaagaa tttgatcaaa gggattaaga atgttttgaa catattgtgc catggcttca
    22261 aactggtctt cttgttcagt ttttaaaaaa taatgtactt gctcagcagg ataaacctga
    22321 cttgccaacc gtgctaattc tttaatatga tccccataaa aaacaagctg atcaatatta
    22381 cctgatgtta agctgtcaat taactgagaa tgaagtatca ctgaatcctt cccaagttct
    22441 ttcatatctg ctagcactgc aatctttttt cctccggggt tctttgctat gttagcaaat
    22501 gtttctaaaa tcagacgcat ggctgttggg ttggcattgt agacatctga taaaatatca
    22561 gcaccattgg ctgctttttt ccactccgta cgattacctg tcaacgtcac agtctgtaaa
    22621 gcagctataa tatcctcgtc tgttactgcc aatagttttc ctacataggc tgctaccata
    22681 gcattggtcg cgttgtattt tcctggtaga ggtagggaca caggtgttgc taacacattt
    22741 gttgtaaacg ttaagctatc tttgtcttct tgaataccag taacgtcaat ttcctgctga
    22801 tttccaaaac ggataaccat ctgattttct ggaagatatg ggtcaataat cggatctccc
    22861 ggagcaatta aaatcccatc tgaattcatg ccgtcaacaa tctgcatttt gccttgggct
    22921 attttgtctc ggcttccaaa atattctaaa tgagcttcgc cgactaaggt taaaactgca
    22981 atacgaggcc ttgcaatttc agacagaaga cgaatatctc ccatgtgatc ttgtcccatt
    23041 tcaagaacaa ttttttccgt atcgtcaggc atgtggagaa cggtgtaagg caaaccaatc
    23101 tcattattgt aatttccttg cgttttgtaa gttttgtaag tggttgatag gacagctcct
    23161 atcatatctt tggtggaagt tttaccattt gagcctgtca cagcaataac atccacgcgc
    23221 atcttatcaa tataataatg ggctagcttt tgaaaagctt ttaggcagtc ttcaacaaga
    23281 aggtgaggtt tccccggcaa ttctttttct gaaaaagtag cgacagctcc attttgaaag
    23341 gctaaatcaa taaactcatg accatccctt tgacctttta agggtaaaaa gaggtctcct
    23401 tttgtaatct ttcggctatc aaattcaatg tgatgcagag gaacgtcgtc taagtcagac
    23461 acattatttt gggcgtcaac tattttagcc acttcgtgta aggttaattt cattttgttt
    23521 tctttctaag aacactagta tttacgatgt cctatctatt atagcacaaa acattagaaa
    23581 aatttctaaa atgtcaaact cccttaatgg ctctactatt cttcctaaaa gagcaacaaa
    23641 aaaaggacga acgtcacatc ttgacgatgt ttgccgttcc atccaagtca atgtttattc
    23701 atctcatagg tttaaatcag gtggctctca cgctggtcaa acatttcttg tgccagtgtc
    23761 accagctctt caatcaaatc tggataagcc aatcccatat tttcccataa gagtggatac
    23821 atagaccatt gggtaaagcc tggcatggtg tttaattcgt taaggtaaac ctgtccgtct
    23881 tgggtcaaga aaaagtcaca acgggacaac ccacaaccac ctaaggcttt aaaggctact
    23941 tcagcataag aacgcatttc tgtcacaata ctctggtcta catctgctgg aatggccatc
    24001 gtaatcttat tgtcaacata ttttgcttgg tagtcgtaaa aatcaacgtc cttgattact
    24061 tctcctggta gagttgattt aaccttatca tttccaagga gaccaacttc aatttctctg
    24121 gcaaccacgc cttgttcaat caagacacga ctgtcatagg tcagggctaa ctgaatcgct
    24181 tttcttaatt ctacttttgt ttgggctttg gaaatcccca cagaagaacc catattagct
    24241 ggttttacga agataggaaa agtaagtctt gctaaagttt caactaaaca agcctccaaa
    24301 tcttgtccgt caatataaac agtataggca acttgtggaa ttccaattga ttctaagaca
    24361 cgtttagtag taattttatc catagctatg ctagaagaca tgacattagt ccctatatat
    24421 ggcattctaa gcacttctaa gaaaccttgg atagagccat cttctcccat aggtccatgt
    24481 aatacaggaa atacaactgc accttcttca tagatatcac ttggttttat tttttgagtt
    24541 agctctatcg tctcatttgt catcaagcgt tctgattccg atggcttttc tgaaaattgc
    24601 tgagttttga taaattggcc catctgagta ataaaataag tttttactaa gaacttatca
    24661 tagttgactg ctcgcatcac actctctgct gaaagcactg agacttcacg ttctgctgag
    24721 cggcctccgt ataatagaac taacgtttgt ttggacatag ctaacctttc ttatctgaat
    24781 tgaatgtttt tttattataa caaaaagatg tagccttttc aaaacctctc tttgcgagta
    24841 aaggcaccaa catgatcatt aaagttcagt acgattctca atagctctca ataatgttac
    24901 ttcatcagca tactcaatat ctgaacctac tgcgagacct cttgccaaac gagttacctt
    24961 aatcccggct ggtttcaaga cacgtgaaat atacatggac gttgcttccc catctgctgt
    25021 ggcattggta gctacgataa cttcgctcac cttaccatcc attagacggg taattaaact
    25081 tttaaggttg atgtcatctg gccccacacc attcataggc gaaatcaagc cgtgaagcac
    25141 atgataatag ccatgatact cttggatttt ttccatggca gaaacatctt tagcatcttc
    25201 tactaccaga atggtcgtct gatcacggct cgtgtctgtg caaatgtgac aaggatcgtc
    25261 atcggtaagg tttccacaaa tcgaacaata ggtcagttct cttttagctg ctaataagtt
    25321 tttagcaaaa tcattgacat cttcatttga cattccaata gtataaaagg ctaatctcgt
    25381 cgccgtcttg ataccaattc ctggaagttt agagtaactg tcaattaact ttgcaatggg
    25441 tgttggataa agcactaagt tctcctttat tgttttccat atatcgccac ataaagcgcc
    25501 ctcacatctt tgccgatata ttcgtaatat gtggatacat aacagcaacc gcaatattat
    25561 cttcgttatt tgtattataa gctactacat ttaagttgta agttactaag gtctgatctg
    25621 aattgtctgt gatacaagtc tcagcagttc caattttagc gtgaatcgtg acagaaccgt
    25681 ttggattagc caagtaaaaa cttttttact cgacttgtta ccttggcctt gagatatttg
    25741 gcttttgttt tttgattcat tttacttttt tagaaaccgc aaaccatcct tatgatataa
    25801 gtttatagga attgtttcat tccatgcaga aatgatgttt actcaattga ctatgctatt
    25861 tttctcaaca agagtgcata aagcgaactc atggttatct ttaagctttc atgctacact
    25921 ttcttcacta aaagtgtatt accaaaagca ttattaagaa atctctaagc acaaaccata
    25981 agacagtttg tacgcctgat gaatctgttg ctgcatcact gtccactttt taccctatca
    26041 aggctatttt tgaaagacaa ctaatgatct gcattatttt gggaactact cccatagcaa
    26101 attcttttta cggatttttt gggaatactc cttttttagg gaataaacta accattattt
    26161 agctaaattt tttaaacttc gcttcttatt ttaccataaa ttataaaaag aagactgaga
    26221 ataggacgcc ctcagtctaa aaaaatattt tctcattgag ttttactctt ttgcggatga
    26281 tagtaaactt taaaatataa aaaactaaca aaaatggctc caccgataat attaccacta
    26341 tagacaaaaa taaaattagt ggcaaaatcc aaccatgttg ctcctccttc aaaaatagca
    26401 gcaggaatga caaaagcatt tgctacacta tgctgaaaac caagggctac aaaggtcatg
    26461 acaggaaacc aagtaccaag gaatttacca gcagcatcat ttgccccata gcatagccac
    26521 aaggccaaac caacaaacca gttacaacca attcctgata ctagtgcctg caaagggctc
    26581 gctgctattt tagcatgggc cacctcaatc acttcttctt taaaaatacc agctgatgtt
    26641 aagcctagaa aatgaccaaa cacaaaagct acaaaaacag ctccaatcac attaaacaag
    26701 gtaatgataa gccaattctt ggcaagctcg ctaaacttaa tttttttggc caacaaagct
    26761 gcagaaacag ccatcatgtt ccctgtaatt aattctccac ccgccattaa aataataatt
    26821 agaccaatcg gaaaagcaca agctcctacg atactggaaa aagcaccaaa agtttctaaa
    26881 ccactggcgg caattctcac gtagagtaaa taaccaaggc taatcatcgc tccaccaata
    26941 aagcctaaga tagatttagc caaaaaagtt ttggtaacct tatgttcccc aatatgaata
    27001 gttgcttcta aaatctgttc tggtgttttc atgatatctc cttttgttac tgtattcaca
    27061 aacacacact ttagtatatc aattttccta aaaaatgcaa gccctttcaa caagaaaggg
    27121 ctcgtcatta tcatataact atctctattt tagtcttagg catttatggt gggaagtttt
    27181 aagttttttg ctaaaaaacg ccatagaact aaagcaagat agaaatccaa tatgccatga
    27241 ataagcgaac ctaagccaat caagcctagc atagaccaaa aataagacat agaagttgct
    27301 gtggttgctg ttaaaatata gacaactaaa aattcagcca agccatgaat gacattaatg
    27361 aaaaacgcaa aactaaaaca ttttactgcc gatgtcatca gttgtggttt tctacttaac
    27421 caccacgctg ccaaaatagc aaacaataaa tgagagagtg ctcttaaaac aataattaaa
    27481 ggaagtccag ctaataaaaa acctaatgtg gtaccaagag cgaccaaaat tgctactgga
    27541 atcgacataa aaatagccaa aaacaaagga acatggctcg ccagagtaaa ggaagctggt
    27601 ccaataataa gcttaagagg catgattatc ggaatcaaaa tcgcaaatgc gaccaaaata
    27661 gaggtataag ctattagttg tgttggtttt ttagtcattt tatctccttt tcaaaaatag
    27721 tataccttaa aaaccaataa ctatcaagac acctgtatct tttggtattt ttaaacgata
    27781 ccttgttgct gataaataat caatagaata ttttagcatg gtattcataa ca
//
LOCUS       DATXIG010000018        25522 bp    DNA     linear   BCT 28-DEC-2023
DEFINITION  TPA_asm: Streptococcus pyogenes strain MGAS11113
            SAMN08555799-rid19682583.denovo.018, whole genome shotgun sequence.
ACCESSION   DATXIG010000018 DATXIG010000000
VERSION     DATXIG010000018.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN08555799
            Sequence Read Archive: SRR6740495
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pyogenes
  ORGANISM  Streptococcus pyogenes
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 25522)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 25522)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-OCT-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-OCT-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001918058.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001918058.2
            Genome Coverage       :: 368x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/12/2023 11:23:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 1,806
            CDSs (total)                      :: 1,769
            Genes (coding)                    :: 1,722
            CDSs (with protein)               :: 1,722
            Genes (RNA)                       :: 37
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1, 1 (5S, 23S)
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 47
            CDSs (without protein)            :: 47
            Pseudo Genes (ambiguous residues) :: 0 of 47
            Pseudo Genes (frameshifted)       :: 30 of 47
            Pseudo Genes (incomplete)         :: 20 of 47
            Pseudo Genes (internal stop)      :: 9 of 47
            Pseudo Genes (multiple problems)  :: 11 of 47
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..25522
                     /organism="Streptococcus pyogenes"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN08555799-rid19682583.denovo.018"
                     /strain="MGAS11113"
                     /serotype="Emm28"
                     /isolation_source="normally sterile site"
                     /host="Homo sapiens"
                     /db_xref="taxon:1314"
                     /country="Finland"
                     /collection_date="2002"
                     /collected_by="National Public Health Laboratory"
     gene            complement(<1..185)
                     /locus_tag="VC122_000813"
     CDS             complement(<1..185)
                     /locus_tag="VC122_000813"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002990048.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEP5640753.1"
                     /translation="MKDKKVATNRRSIIFISVPKSSIITTNLLEPEVGINEKLAKNIA
                     VTIAENDRDADIISVKF"
     gene            complement(501..878)
                     /locus_tag="VC122_000814"
     CDS             complement(501..878)
                     /locus_tag="VC122_000814"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017647278.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system HicB family
                     antitoxin"
                     /protein_id="HEP5640754.1"
                     /translation="MLIYPATFTQDTDYIMVTFPDVPEAITQGEDFQEAYEMAVEVLG
                     FALEDYTDYPKASAVSDLKEQYPDSDIALIGIDMVAYMKKYHSKKVRKNVTIPEWLND
                     AAEDKNLNFSQVLTEALELKLQA"
     gene            complement(930..1115)
                     /locus_tag="VC122_000815"
     CDS             complement(930..1115)
                     /locus_tag="VC122_000815"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012679315.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system HicA family
                     toxin"
                     /protein_id="HEP5640755.1"
                     /translation="MPMTPKQMIKLLKKNGFYEVSQNGSHKKFRDNNGHQTIVPMHNK
                     DLGKGLEDTILKQAGLK"
     gene            1178..1645
                     /locus_tag="VC122_000816"
     CDS             1178..1645
                     /locus_tag="VC122_000816"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000863702.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="terminase small subunit"
                     /protein_id="HEP5640756.1"
                     /translation="MRIKYTKGGDGKLSKLTLKQKRFADEYIISGNAMESAIKAGYSH
                     NYSKAQSHKLLENVGIKEYLDERFEKMDSKTIADQKEVLQYLSSVMRGEQQEKTLISI
                     GELGQEIVDIDVGAKDRLKAAELLGKRYRLFTDKIEAEVQGTVVFVNEDDIPD"
     gene            1623..2930
                     /locus_tag="VC122_000817"
     CDS             1623..2930
                     /locus_tag="VC122_000817"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014635515.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PBSX family phage terminase large subunit"
                     /protein_id="HEP5640757.1"
                     /translation="MKTTYQTKPKLEIKVDLPKTIGIGYGAFWRSKNFYRVVKGSRGS
                     KKSKTTALNFIVRLLKYPWANLLVIRRYSNTNKQSTYTDFKWACNQLKVTHLFKFNES
                     LPEITVKATGQKILFRGLDDELKITSITVDVGLLCWAWFEEAYQIETEDKFSTVVESI
                     RGSLDAPDFFKQITVTFNPWSERHWLKRVFFDEETKRADTFSGTTTFRVNEWLDDVDK
                     RRYEDLYKTNPRRARIVCDGEWGVAEGLVFDNFEVVDFDVEKTIQRVKETSAGMDFGF
                     TQDPTTLICVAVDLVNKELWLYNEHYQKAMLTDHIVKMIRDKNMHKSYIAADSAEKRL
                     IAEIKSKGVSGIVASLKGKGSIMQGVQFMQGFKIYIHPSCEHTIEEFNTYTFKQDKEG
                     NWLNEPIDKNNHVIDAIRYALEKYHIRSNESNQFEVLRAGFGY"
     gene            2942..4444
                     /locus_tag="VC122_000818"
     CDS             2942..4444
                     /locus_tag="VC122_000818"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011017863.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage portal protein"
                     /protein_id="HEP5640758.1"
                     /translation="MYTESFRDSTGKTKTLEFRFHREARMRYQAESLESLLTEKYKLL
                     REMIEHHDKVQKPRIQELLDYAEGNNHTISEIGRRKDDDMADVRAVHNYGKYISTLKQ
                     GYLVGNPIRVEYIDGTEQQQDLLKDLSVKNNFHQLNRRLVKDLSKVGRAFELIYRSMD
                     DKTEVVRLDPREVFVIYQNNLEQSSLAGVRYYNKNQLDGTTKIVELYTDNKILKFEYD
                     GDLTLIREASSHAFGSVPITEYLNTDDGMGDYETELSLIDLYDAAQSDTANYMQDLSD
                     AILAIFGRVSFPHDVQTAEQRIEFVKVMRKARLLNLEPPVDQDGREGSVDAKYLYKQY
                     DVQGTEAYKNRIVSDIHKFTNTPDMTDSKFAGQQSGEALKWKVFGLDQERVDMQALFE
                     QSLKRRYKLIARVSQLLKEIDDFDISKLKITFTPNLPKSLQEKIEAFKALGGELSQET
                     AMAITDIVEDAKKEISLINSESKSRSQLAQKLEETSRLTDRELAHDHQKE"
     gene            4425..5987
                     /locus_tag="VC122_000819"
     CDS             4425..5987
                     /locus_tag="VC122_000819"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012560958.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage head morphogenesis protein"
                     /protein_id="HEP5640759.1"
                     /translation="MTTRKSKYWRDRIKKEMDAKEADDISLEQSMKQLHDYHFRNIEK
                     EIESFYQRYADKEKIDLSEARKRASELDISAYQKKAKELIAKAEKLRKEGRTVTKADF
                     THQENADMAIYNLAMKTNALELLRLNIDLEMQELANGEHKLTKKFLDEGYRKEVEFQA
                     GLLGLSVASQANVKNLADAVINANFKGAKWSDNIWDRQDKLRSIISQSVQSAILKGKN
                     GLTIARDIRREFDVSASYAKRLAITEHARVQMEVGRLSMAENGFAMFDILPEPKACDV
                     CKDIAKHGPYHLDKWRIGENSPPFHPYCRCAVVGLDKKQEEKVNDKSEKFENITPNLM
                     PLFSKFASKITDLQRKIVYSADLADTGYIRTPHAFDINNTLRNKGYNYLNVDDKLITD
                     TLDSVISINSTPKNIKVYRFDDFELLGSINEQNNNIFDSGNFMDKLNQGGLSYTNDGY
                     TSASYDVKKNVMGYRPIKTEIKVPKGSHVYLTDNEEESEIILPRGTKYDIINAKINEY
                     EEIEITMEIRKE"
     gene            5991..6176
                     /locus_tag="VC122_000820"
     CDS             5991..6176
                     /locus_tag="VC122_000820"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002988389.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEP5640760.1"
                     /translation="MDFSDFLNKKQKEWDESHPIPDFSAMSDEELLYQPMSEALVSER
                     FAKELSKEVRKRNLLSK"
     gene            6246..6560
                     /locus_tag="VC122_000821"
     CDS             6246..6560
                     /locus_tag="VC122_000821"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011284859.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEP5640761.1"
                     /translation="MNRESKPGMESVKIGGLTYSVEKASDLQGKDGNWGLIHYKTQQI
                     KLDDSLTEQLEDQTLIHEIVHGILVEAGYTNHEEDQANRIGLILYQVLQDNDFSWLYK
                     GE"
     gene            6563..6829
                     /locus_tag="VC122_000822"
     CDS             6563..6829
                     /locus_tag="VC122_000822"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011054805.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEP5640762.1"
                     /translation="MNKRIKKKRKLETAVVMLVAENAIQAKALRNQNKQIAELRAIIQ
                     QNVQAINREFVTAKNTILDNQLAIKLIGDDVGHIKQNYKRKWRK"
     gene            6973..7506
                     /locus_tag="VC122_000823"
     CDS             6973..7506
                     /locus_tag="VC122_000823"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002988401.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4355 domain-containing protein"
                     /protein_id="HEP5640763.1"
                     /translation="MAEETQVVEMVEEQVVPEAKQPQDEKKYTDADVDAIIDKKFAKW
                     KSEQEAEKSEAKKMAKMNEKEKADYEKQKLLDELQELKNDKTRNELTAVARQMFAESE
                     INVNDDVLGLVVTLDAEQTKANVTTLANAFAKVIADDRKALVRQTTPSTGGGLRKQTN
                     YGANLASKAAQQSTKLF"
     gene            7516..7896
                     /locus_tag="VC122_000824"
     CDS             7516..7896
                     /locus_tag="VC122_000824"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002984386.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="head decoration protein"
                     /protein_id="HEP5640764.1"
                     /translation="MNKRKVTTSKEILHNLDYEAISVTLDSKKIGKEVVPAGTVLAGV
                     SGSVFEDRKQKVKAVDNSAVESEDNICGILLTDVDLTNGDAVGSCIYRGTINADKLAD
                     SSIAGKYEVLEEELPHIVFIKGGK"
     gene            7899..8981
                     /locus_tag="VC122_000825"
     CDS             7899..8981
                     /locus_tag="VC122_000825"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002988406.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="major capsid protein"
                     /protein_id="HEP5640765.1"
                     /translation="MALIHEIITSENIKGFYNAKNENVGNTLGENAFPPKQQLGLKLS
                     FIKGAAGKPVTLKAAAFDTKVPLRDRMTVELLDEEMPFFKEAMVVKEADRQQLSLLAQ
                     TKNQELIDTALAAIYNDKVTLIAGAKARLEAMRMEVLSKGKIHVASNGVMKDFDYGLD
                     SSQTTKPDTKWEQVDTATPLKDIEKAIEVMAERGFVPEAIIMNSKTLSLIKNASNTLD
                     VVKPMAPDGAAVTKGDLETYIADELGLKILLKDGMFVGDDGKAKKYFPDGFATLVPNG
                     NLGYTVFGTTPEQSDLLGGEATDAEVSIVETGIAITTTKTTDPVNVQTKVSMIALPSF
                     ERLEEVQIINATESEEETKKENSFEM"
     gene            8991..9233
                     /locus_tag="VC122_000826"
     CDS             8991..9233
                     /locus_tag="VC122_000826"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002988409.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEP5640766.1"
                     /translation="MPRVIRAFKDKVTKVVYEVGDIYSGDRVEFLTEGGVLEPSVDFD
                     KLKVSEIKSKLDELSIEYDAKLKKSELLKLLKQTIG"
     gene            9247..9600
                     /locus_tag="VC122_000827"
     CDS             9247..9600
                     /locus_tag="VC122_000827"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002984392.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage head-tail connector protein"
                     /protein_id="HEP5640767.1"
                     /translation="MDAVNTSSVISNVKLDLGILDSQQDDLLNMLLKRVTDHFKAKYG
                     AVEIDSAFSFVLEDCLIARFNRRGAERAKSESVEGHTTTYYDFLDEFEPYDAMIMAKL
                     NLIKDKSRKGGLYFL"
     gene            9597..9905
                     /locus_tag="VC122_000828"
     CDS             9597..9905
                     /locus_tag="VC122_000828"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003052388.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEP5640768.1"
                     /translation="MRYADRVTFVKTTDEQYNPDLGEYTHTEVISITKPCFVMDMGME
                     KSVQIFGDYQKDRKVIYLKQPYTKAFDYCEYEGKRYKAQANKLGAIVFYLEGDDSIGG
                     "
     gene            9886..10251
                     /locus_tag="VC122_000829"
     CDS             9886..10251
                     /locus_tag="VC122_000829"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002984399.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HK97 gp10 family phage protein"
                     /protein_id="HEP5640769.1"
                     /translation="MILLVADISLKVVGTAGLKKKLELIVKKDAVKKIVRDNGTQLQR
                     KMINKAVFTKGYSTGATRRSITMQIGDGGLSVKVKPGTHYAGYLERGTRLMSKQPFVL
                     PALKEQKVKFRKDLEALVK"
     gene            10248..10637
                     /locus_tag="VC122_000830"
     CDS             10248..10637
                     /locus_tag="VC122_000830"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010922220.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage capsid protein"
                     /protein_id="HEP5640770.1"
                     /translation="MIKTRDQSIFDEMFKRIQSLGFKVYDYKPMTEVPYPFVEMESTD
                     AEYIPNKDDIKGSVELMLSVWGVQKKRKQVSDMASAIFSQAMSVARTDGFCWSFNIRQ
                     SSIQMLDDTTTVTPLKRAIVTLRFNLR"
     gene            10692..11315
                     /locus_tag="VC122_000831"
     CDS             10692..11315
                     /locus_tag="VC122_000831"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002988424.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage major tail protein, TP901-1 family"
                     /protein_id="HEP5640771.1"
                     /translation="MSKAQEQTKQLEAKQGIHSILLFRLLKEASSEVATKLAFQTEHE
                     VGKSRDVDGQKTKDGIIQSVGALEYDFKATSILAKGDELAAKLEKAMEDGELVEIWDI
                     DSEEISKNGDSDNKLAKVWGINKNGTNGGNGKCLATYYQGYISSFSAKKNAEENIVIE
                     MEFAINGVGKKGFATLTDAQKAAVQYAFKDTTKSNSEETKKENSFEM"
     gene            11369..11722
                     /locus_tag="VC122_000832"
     CDS             11369..11722
                     /locus_tag="VC122_000832"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002990023.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tail assembly chaperone"
                     /protein_id="HEP5640772.1"
                     /translation="MQLEIKGKTHNVKFGTRFVAEMDKNHIAERQGFKFGAGLQSSVP
                     FLIDHSVVTLAEVIYTGTITEPPRPSLNDIYDYIDEVEDIEKLFDDVLDELRQSNASK
                     LFMAQVEKDLAEAKA"
     gene            11797..12093
                     /locus_tag="VC122_000833"
     CDS             11797..12093
                     /locus_tag="VC122_000833"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002984407.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEP5640773.1"
                     /translation="MTDINEIGRLTLYEYDLLMTGKALAAVDESHKAHKQAWINHQVT
                     ATKLVGGKKNKKEVPVYKKFKDFFDYEEEIRKITQEIDEGYDKKGMDLLLKANL"
     gene            12108..15743
                     /locus_tag="VC122_000834"
     CDS             12108..15743
                     /locus_tag="VC122_000834"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014635504.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tape measure protein"
                     /protein_id="HEP5640774.1"
                     /translation="MGESYSVEAVLTAVDKTFGKTLQSAIRSIEGLEKRSTGFSSVSQ
                     KASSMFKSMLGANLAGQAISAMTRTVSSGLGSMLGEMNSSAKAWKTFDANLADIGFGK
                     KQILAAKTAMQDYATKTIYSASDMASTYAQLAAVGVKDTGKLVKAFGGLAASAENPKQ
                     AMKSISQQMTQAVGRPTVAWQDFRIMLEQAPAGMAKVAKSMGKNLDELVADIQAGRVK
                     TSDFLEAVKKAGNDKSFQKMATEFKTVDQAIDGMREGLSNKLQPAFEKVNQFGIRAIE
                     AIGKQLDKVDFSKFASNLGKFLEGINIDKIVSNISSAISSVTSKVKEFWDGFKQTGAI
                     SAFSGALQSVWEALKNVASAMSGGNWKTFGATVGGTVKHVSNFAKAVSDVLGKMDPGR
                     LRSWIATFAAVAGGFKLFEKLTGQSVIGSFLDKIGSKFGLFGNKAKEGTDKASNGARR
                     SGGIISQIFSGLGNIVKSAGTAISTAAKGIGAGIKTALSGIPPIISSLGTAISTVAQG
                     IGTGLAIAFKGLGSAIAMVPPTTWLALGAAILMVGAAFALAGTQADGISQILRTVGDV
                     VVQILQQVTDSLATLLPIIANAIGSMLPIVAGAISQIVGAVAGGLSQLVIAVSTGASL
                     VIGAFTGLLGGISGVINSISAVIQSLTGVITAVFNGIATVISSVGSAIKDVLTGLGTA
                     FEGFGNGVKSALEGVGAVIESFGSAVRNVLDGVANILDSMGTAALNAGRGVKEMAKGI
                     KMLVDLSLGDLVATLAAVASGLGKMASSAGEMTTLGSAMSKVANGMTRLATSATIAIT
                     GLTVFATTMATIKTAVATLPPVLTMAASGFTTFTTQAVAAVTGLAAINAPITMFKAQL
                     MTITPALAQAGAGFAAFVAQSSTFSTGLASAGPTIAAFNANLMSLSATTGALVASIAG
                     LSAVLSVVSAGFSQIGASATATVGQIQAFASSTTVVSSAFASMQSMIQSAMAAIVSSI
                     ITSFNQAASQMQSILSRMLSQARTFGSQLEQQMRQSGQRSGQNLARGLSSQQSAVINA
                     ISSMVNAAVSRANAGAGPMRQAGAYIGQGLAQGMYSALGAVTAAANALVAQAERAARA
                     KAMIHSPSRLFAKRVGQYIPQGVAMGIDKNADVVDDSVGGLFDSINSFDFNIADRLAS
                     IGAKFQGVIKSESSQSLSQQQEFVHTAQPAYINFSLGGNEYEAFVSDITNQQAKIEKI
                     RLKRSSW"
     gene            15775..16554
                     /locus_tag="VC122_000835"
     CDS             15775..16554
                     /locus_tag="VC122_000835"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011017848.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail family protein"
                     /protein_id="HEP5640775.1"
                     /translation="MYEFNDTIRGTPKVTFNLKTTIGEKVLEEELNNDLGTFRTLTVS
                     GRDVVDLEHQTTSVLGRNGEYFHNATVEVRKLEIKAKITGKDNQSMRLQYEKLNRLIV
                     SHNQVFLSFSDEPDRNYLGIFKSKDVPEEVSNEQIIGLTFICYNPFKMSDVKTKKGTS
                     IQNGGLFQTKPIITLNLSSPTKEIKLLHVESQKYIRLTGTYTTDEIKIDMATGKITQN
                     GRNILGDLDMINSRYFELLPGNNTLQCANAAITAEFREVYL"
     gene            16551..18605
                     /locus_tag="VC122_000836"
     CDS             16551..18605
                     /locus_tag="VC122_000836"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002988436.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HEP5640776.1"
                     /translation="MIYLFDKLERLVATVGTDDLLSWHFKVKNNDWDQASFEVPVDYD
                     VEPFVYFGFFNYDPNQKEDVFKLFKVIDYNLEDSKFYKGLDKAESDLDTIAIIKDKRF
                     RQSSADACIDGALEGTGYQVGKVEGITNVRTLSYYYISPRAALIKIVEAFNCEFNVRY
                     TFINNKITSRYIDLKKRFGKPTGKQFEHGNNLLKVVYEESTDDIVTCLIGRGKGEEIQ
                     HEEAEPKDVEGHLPQEERRQGYGRRIEFTDVVWSVEKGDPIDKPAGQNFVALDSAREE
                     YGLSQNGELKHRWGVFVNEEIEDKTELLKATWEELQRLSIPIRIYKAEILDIGPETWK
                     GDSVAIIYDEVKIAFETRVDEIDIDKLNFNRSVVTLGDYSVVQNRESRSRKEAVQNMI
                     DESLETITDLGMTFQEFLQGIEKRIETGKKEMEDNWRKVNLEFDNFKKKVEQEGLQFN
                     TLKEQIKEVDERTDKELEEFRATLKNLALPEEAIKKITEAIKVDDIPSLKQNFDDLKN
                     KVSETSETARLNAEIIGTDGKTRYNKNLLVGDPNRVKTYDQDYIEVEANDGGFKRGET
                     YTISFSQTCELLKKVAITLTQINNKGVKLVLTPTKAKMDAQTFEVTKDKQSIEVYPLS
                     YTGVLTGDWYKSKSVEINASEAQELALEMAYKEIADAKGATIIGKQSDKPKIILDGRR
                     DR"
     gene            18602..19816
                     /locus_tag="VC122_000837"
     CDS             18602..19816
                     /locus_tag="VC122_000837"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008089917.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEP5640777.1"
                     /translation="MTSVETIPIKIVFDRKDASEWQSTNPVIDEGELVVELDTHKLKV
                     GDGKTSYNDLPYYEGPQGESITKVQLSENGDLSVWIGDKETKLGNIKGQKGDKGTSIT
                     DITKDGETLTIKLSDDTQKIFNIPNGQKGDRGKGVESARIDESGHLKLKIEEESELDL
                     GNVKGESGPKGDSITITNHKRVSDGTQVSFSDGTQIVVPKGDTGDVNGINLEDYVKKS
                     ELKNVGSADVKAINDFLGLSQKVFTSSYSYTDSLLKSYAKPSYSASWYVNESTVSTKN
                     GDKVLITIHNTTTQADNYLEVAVTYVGANYVTATSTGRLLTTPGEVKVVTKKQAEKDY
                     AAKKHKHEISDIAGLNERLSGYLRQADIQSQLNNIGKLKDTQTGQYLEVKVVDKGQVP
                     SNTSGMIVFERS"
     gene            19803..20132
                     /locus_tag="VC122_000838"
     CDS             19803..20132
                     /locus_tag="VC122_000838"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011284842.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEP5640778.1"
                     /translation="MRGHNLLKDLNNIIINNKTIDRIICNNTIVYQRSWDLLFDGLLT
                     SAGKKLDIYPFYKIVINNAFFVKTPTTKNNTIFLAGHKILALQDSHFCFFRALEKSFF
                     VKIYGKN"
     gene            20143..22038
                     /locus_tag="VC122_000839"
     CDS             20143..22038
                     /locus_tag="VC122_000839"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002990016.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gp58-like family protein"
                     /protein_id="HEP5640779.1"
                     /translation="MSRDPTLLIDESNLTIGSDGRAYYTFTADGDTKSVKIANNKCIG
                     TTRFNQLMIERGGKPTNYVAPVVVEGTGNPTGLFKDLKEISLELTDTKNSKLWSKIKL
                     NNQGMIEEYYNGTIKSEIIKTAEGTQQRISSETDKKLALINETVSGIRREYQDADRQL
                     SSSYQAGIEGLKATMANDKIGLQAEIQTTAQGLYQRYDNEIRKLSAKITTTSSGTTEA
                     YESKLDGLRAEFTHSNQGMRVELESKISGLQSTQQATARQISQEISNREGAVSRVQQG
                     LDSYQRRLQNAEGSYSSLQQTVSGLQSDVNSPNSKLNSRISQLASQIDQRVTRADVTS
                     IINQSGDSIKLAIQRAGGIDAKMSAKEIVSAINLNGYGVRISGERIALDGNTTVNGAF
                     GAKIGEFIKLKADQIIGGTIDANKINVINLNASSIVGLDANFIKARIEYAITSLLEGK
                     VIKARNGAMTIDLQSGQINHYTNESAMRRIDSSTASQFIKMTKSGFISEIGNMQAAMT
                     VIGSNSDGSENHENKTFGGIRIWNGKSSYQSTSFVELVGNRVAIYGNKNRSPWLFDST
                     TSGYAYLIPQNESGIKHAIGRADRKIDQIHVGDIYVQGERVAMMLKDLANRIGYIGTG
                     GWANRIG"
     gene            22052..22213
                     /locus_tag="VC122_000840"
     CDS             22052..22213
                     /locus_tag="VC122_000840"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002987333.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEP5640780.1"
                     /translation="MQEKLLGKIINDLTLKVANLTLENAQLKAQHEIELEELNAQLDE
                     ATAPKEEGK"
     gene            22216..22833
                     /locus_tag="VC122_000841"
     CDS             22216..22833
                     /locus_tag="VC122_000841"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011054861.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEP5640781.1"
                     /translation="MRNWKVTGNYPQWDGTGAVIDTQVIITDDRGAVIAEKIKKDLRT
                     ANDAEIIEAALEEFKKTAYVEIAMGEAVQKVDDLEKISQETAKTAKTAQTAAGLAKVS
                     AERTQKMINLQTIHMLTSGGKIDPDIYKGMLELIEPAKKGEYQAYDVFTVVDSTKEED
                     GEAGEGNLVFVHVNEPFTYEAQTLKDLESEDKVTVIKYADLVKQD"
     gene            22844..23140
                     /locus_tag="VC122_000842"
     CDS             22844..23140
                     /locus_tag="VC122_000842"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011054732.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEP5640782.1"
                     /translation="MIDFVQIGAFCGAALSILGVWGFIVNPFKKAMEANEFAMAQLKD
                     SIKELAYELKNLDREREITKKIIDRHEERLGRVEDEVIINKERIITLFKKGEEK"
     gene            23137..23322
                     /locus_tag="VC122_000843"
     CDS             23137..23322
                     /locus_tag="VC122_000843"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011054731.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEP5640783.1"
                     /translation="MNNWFKKVAIKTIKTMAQTAVGLIGSSMLITDINWPTMLSAVLL
                     SGLTCILMNVSQIKEEE"
     gene            23434..24768
                     /locus_tag="VC122_000844"
     CDS             23434..24768
                     /locus_tag="VC122_000844"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017768203.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysin"
                     /protein_id="HEP5640784.1"
                     /translation="MATYQEYKSRSNGNAYDIDGSLGAQCWDGYADYCKYLGLPYANC
                     TNTGYARDIWEQRHKNGILNYFDEVETMQAGDVAIFMVVAGVTPYSHVAIFDSDAGSG
                     YGWFLGQNQGGANGAYNLVKIPYSTTYPTAFRPKVFKNAVTVIGNIGLNKGDYFIDVS
                     AYQQADLTATCQQAGTTKTIIKVSESLAWLSDRHQQQANTSDPIGYYHFGRFGGDSNL
                     AQREADLFLSNLPTKKVSYLVIDYEDSASADKEANTNAVIAFMDKIANAGYKPVYYSY
                     KPFTLNNIDYQQIIAKYPNSIWIAGYPDYEVRTEPLWEFFPSMDGVRWWQFTSVGVAG
                     GLDKNIVLLADDSSKVDIPKIDKPQSQLTFNQKLDTNTKLDNSNVPYYEATLRTDYYV
                     ESKPNASSADKEFIKAGTRVRVYEKVNGWSRINASQSDQWVEDKYLSNATQV"
     gene            complement(24844..25518)
                     /gene="speC"
                     /locus_tag="VC122_000845"
     CDS             complement(24844..25518)
                     /gene="speC"
                     /locus_tag="VC122_000845"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010921949.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="streptococcal pyrogenic exotoxin SpeC"
                     /protein_id="HEP5640785.1"
                     /translation="MITVILISTISPIIKSDSKKDISNVKSDLLYAYTITPYDYKDCR
                     VNFSTTHTLNIDTQKYRGKDYYISSEMSYEASQKFKRDDHVDVFGLFYILNSHTGEYI
                     YGGITPAQNNKVNHKLLGNLFISGESQQNLNNKIILEKDIVTFQEIDFKIRKYLMDNY
                     KIYDATSPYVSGRIEIGTKDGKHEQIDLFDSPNEGTRSDIFAKYKDNRIINMKNFSHF
                     DIYLEK"
BASE COUNT         8640 a         4397 c         5612 g         6873 t
ORIGIN      
        1 aaaaatttta ctgatataat gtcggcatct ctatcatttt cagcaattgt aaccgcaata
       61 ttctttgcta gtttttcatt gataccaact tccggttcta ataaattagt agtgatgatg
      121 gaagatttag ggacagagat aaaaataatg gaccgcctat tagttgctac ttttttatct
      181 ttcattagtt ctttattatc tttcatttct ctattcttta gtaagactga tactgattta
      241 atttcaattt tagtagtttc aagttggttg agctcaacag taatgatgtt cctaagttcg
      301 ttctttgtgc taagaacatt aattttgtta gttgaaactt ataataattt caaaaataaa
      361 taggtcatca cattgtggtg acttttttgt tatggtccac gacatggtta cttattagtt
      421 acaaacgttg aaattacaat gtttcgatag gtcaacgtcg tggtttttta ttatgtaaaa
      481 aagaaccaca acagtggctc ttatgcttgt aattttaatt caagcgcttc agtaagaact
      541 tgagaaaagt tgaggttttt atcttctgct gcgtcgttta accattcagg gatagtcacg
      601 tttttgcgga cttttttgga gtgatatttt ttcatatagg caaccatatc gatgccaatt
      661 aaagcaatgt cggaatcagg atactgttct tttaaatcag aaacggcgct agcctttgga
      721 tagtcagtat agtcttcaag ggcaaaacct aagacttcga cagccatttc ataagcctct
      781 tgaaagtctt caccttgagt aattgcttca ggtacatctg gaaatgtaac catgatatag
      841 tctgtgtctt gtgtaaatgt agctggataa attaacataa tgatcctcct ttgattactg
      901 tgagataagc acaccatctg gcaagcggat tattttaaac ccgcctgctt tagaatggta
      961 tcttcaagac cttttccgag gtctttattg tgcattggaa caatggtttg atgtccgtta
     1021 ttgtctcgga attttttgtg acttccgttt tgactgactt cgtagaaccc attctttttt
     1081 agcagtttta tcatctgctt tggggtcatt ggcatattgc ttacctcact ttctatactt
     1141 atattataca cataaacaat gtagatgtca atgcaaaata cgcataaaat acacaaaagg
     1201 cggtgatgga aaattgagta aattaacact aaaacagaag cggtttgcag atgaatacat
     1261 catctcaggg aatgctatgg agtcagctat aaaggctggg tatagccata attattccaa
     1321 agcacagtcg cacaagctat tggaaaatgt tggaattaaa gagtatcttg atgaacgatt
     1381 tgaaaaaatg gattccaaga caatagcaga ccaaaaagag gtattgcaat atctttcttc
     1441 ggtcatgaga ggtgagcagc aagaaaaaac actcatcagc attggagagc ttgggcaaga
     1501 aatagttgat attgatgtag gagcaaaaga caggttgaaa gcagctgaat tgcttggtaa
     1561 gcggtacaga ttgtttaccg ataagataga ggctgaggtg caaggaacgg tggtgtttgt
     1621 caatgaagac gacataccag actaagccaa agctagaaat caaagttgat ttacctaaaa
     1681 ctatcggtat aggttatggc gctttttggc gctctaaaaa tttttatcga gtagttaaag
     1741 gcagccgtgg atctaaaaaa tctaaaacga ctgctttaaa ttttatcgtc agactgctga
     1801 agtacccttg ggctaactta ttggtcatcc gtagatactc aaacactaac aaacaatcta
     1861 cttataccga ttttaaatgg gcgtgtaatc aattaaaggt tacacacctt tttaagttta
     1921 atgagagctt gccagagata accgtcaagg caacaggaca aaaaatctta ttccgtggtc
     1981 ttgatgatga actcaagata acatctatta ctgttgatgt cggtttgctc tgttgggcat
     2041 ggttcgagga ggcttatcaa attgagaccg aagataagtt ttcaacagtt gtcgaatcaa
     2101 tccgcggtag tttagatgct cctgattttt ttaaacaaat aacagtcacg ttcaacccgt
     2161 ggtcagaaag acattggctt aagcgtgtct tttttgacga agaaactaaa cgggccgata
     2221 cattttctgg gactacaaca tttagagtaa acgaatggct tgatgatgtc gataaaagac
     2281 gctacgaaga tttgtacaag actaatccaa ggcgggctag aatcgtctgt gatggtgaat
     2341 ggggcgttgc tgaaggtctt gtttttgata actttgaagt cgtagatttt gatgttgaaa
     2401 aaacaattca acgtgtcaaa gagacttcag ctggtatgga ctttggtttt actcaagacc
     2461 ctacaactct tatatgtgtt gcagttgacc tcgtaaacaa agaattatgg ctttacaatg
     2521 aacattatca aaaagctatg ttgacagatc atattgtcaa aatgataaga gataaaaata
     2581 tgcataaatc ttatatcgca gctgatagcg ctgaaaaacg tctaatagca gagataaaaa
     2641 gcaaaggtgt atctggtatt gttgccagtc ttaagggtaa agggtctatt atgcaaggag
     2701 ttcagtttat gcaaggtttt aaaatataca tccacccttc ttgtgaacac acaatagaag
     2761 agtttaatac ttacactttt aagcaagaca aagaaggtaa ttggttaaac gaaccgatag
     2821 ataagaataa ccacgttatt gatgcgatta gatatgcgct tgaaaaatac catatcagaa
     2881 gcaacgagtc aaatcagttt gaagttctta gggctggttt tggttactag aaaggaaaat
     2941 aatgtacaca gaatcattta gagatagtac gggaaagact aaaacattag agtttaggtt
     3001 ccaccgtgaa gctcgcatga ggtatcaagc ggaaagtcta gaaagcttgt taaccgaaaa
     3061 atataagcta ctccgtgaaa tgattgaaca ccacgataaa gtccaaaaac cacgcataca
     3121 agagcttcta gattatgcag agggaaataa ccacaccatc agcgaaatag gccgtaggaa
     3181 agacgatgac atggctgatg ttcgtgctgt gcataactat ggtaagtata tttcaacgct
     3241 caaacagggc tatttggtgg gtaatcctat tcgtgtagag tatattgatg gtaccgagca
     3301 gcagcaagac ctattaaagg acctatctgt taaaaacaat ttccaccagc tgaaccgcag
     3361 attagtaaaa gacctatcca aggttggtcg agcgtttgaa ttgatttatc gcagcatgga
     3421 tgacaagaca gaggtcgtta gactagatcc acgggaagta tttgttatct atcaaaataa
     3481 cctagagcaa tcaagcttag ctggtgtgcg gtactataac aaaaatcaat tagatggtac
     3541 tacaaaaatt gtcgagcttt acaccgataa taaaatcctg aagtttgaat atgatggtga
     3601 tttaacactg attagggagg cttcgtctca tgcgtttggt tcggtaccaa tcacggagta
     3661 tctcaacaca gatgacggca tgggcgacta cgagacagag ttgtctttaa tcgacttgta
     3721 tgatgcagct cagtctgata cagctaacta catgcaagat ttgtcagacg caattctagc
     3781 cattttcgga cgtgtgtcat ttccgcatga tgttcagaca gccgaacagc gtatcgaatt
     3841 tgtgaaagtg atgcgtaaag ctagattgct taacctcgag cctcctgtcg accaagacgg
     3901 gcgtgaggga tctgtagatg ccaaatatct atataaacaa tatgacgtac aaggaaccga
     3961 agcctataaa aatcgtattg tgtccgacat ccataaattt accaatacgc cagacatgac
     4021 agacagtaag tttgccggtc aacaatccgg agaggcgttg aagtggaaag tgtttggtct
     4081 tgatcaggag cgtgtcgaca tgcaagcttt atttgagcaa tctcttaaac gtaggtacaa
     4141 actaatcgct cgtgtaagcc aactgcttaa agagattgat gactttgaca tcagcaagct
     4201 taaaatcaca tttacgccaa acctacctaa gtcgctacaa gaaaagattg aagcctttaa
     4261 agcattgggt ggagagttgt cgcaagagac agctatggct attacagaca tcgtggaaga
     4321 tgctaagaaa gaaattagcc ttatcaatag cgagtcgaaa tcacgtagtc aactagcgca
     4381 gaagttagaa gaaaccagta gattgactga tagggagtta gctcatgacc accagaaaga
     4441 gtaaatactg gcgtgaccgt atcaagaaag aaatggatgc taaagaggca gacgatatct
     4501 ctcttgagca atccatgaag caattgcacg attatcattt caggaatatc gaaaaagaaa
     4561 ttgagtcgtt ttatcaacgt tatgctgaca aagagaaaat agacctttca gaagcccgta
     4621 agagagcttc tgagcttgat atttctgctt accagaagaa agctaaggaa cttattgcaa
     4681 aggctgagaa gctacgaaaa gagggtagaa cggtaacaaa agccgacttc actcatcaag
     4741 aaaatgcaga catggctatt tacaacttag ccatgaaaac caacgcttta gaattgttgc
     4801 gcttgaatat cgacttggag atgcaagaac ttgccaacgg cgaacacaag ctaaccaaga
     4861 aatttcttga cgaaggttac cgtaaagagg ttgagttcca agctgggcta ttaggattat
     4921 cagttgctag ccaagcgaat gtgaaaaact tagctgatgc cgttattaat gctaatttca
     4981 agggagcaaa atggtcagat aacatttggg acagacaaga taagttacgt agcatcatat
     5041 ctcaaagcgt ccaaagtgct atcctaaaag gtaaaaatgg cttaactatt gcaagagata
     5101 ttagacgaga gtttgatgtg tcagcatctt acgcaaagcg actagcgata acggagcatg
     5161 caagggttca gatggaagtt ggtagattat ccatggcgga gaatggcttt gctatgtttg
     5221 atatattgcc tgagcctaaa gcatgtgatg tttgcaagga tatagctaag catggtccat
     5281 atcaccttga caagtggaga ataggggaaa actctccgcc gtttcatccg tattgtcgtt
     5341 gtgcagttgt aggattggat aagaaacaag aagaaaaagt gaatgataaa tctgaaaaat
     5401 ttgaaaacat aacaccgaat ctcatgccat tatttagtaa gtttgcaagt aaaataacag
     5461 atttgcaacg taaaatagtg tactctgctg atttagcaga taccgggtac attagaacac
     5521 cgcatgcgtt tgatataaac aataccttgc gaaataaagg ctacaattat ctaaacgttg
     5581 atgataaact aattaccgac acattggata gtgtcatctc gataaattca actcccaaaa
     5641 atataaaagt ataccgattt gatgattttg agttattagg ttcaatcaac gaacaaaaca
     5701 ataatatttt tgattcaggt aattttatgg ataaattaaa ccagggagga ttatcataca
     5761 ccaacgatgg atatacttct gcaagttatg atgttaaaaa aaacgtgatg ggatatcgtc
     5821 ctattaagac tgaaataaaa gtacctaaag gaagccacgt ttatcttaca gataatgagg
     5881 aagaaagtga aattatacta ccacgaggga caaagtatga tataattaat gcgaagataa
     5941 atgaatatga agaaatagaa atcactatgg aaataagaaa ggagtaagcg atggattttt
     6001 ctgatttttt gaataaaaaa caaaaagagt gggatgaatc tcatccaatt cctgacttta
     6061 gtgcaatgag tgatgaagaa ttgctttatc agccaatgag tgaagcttta gtgtctgaaa
     6121 gatttgctaa agagttatct aaagaggtta gaaagcgtaa tttattatca aaataaaaac
     6181 atattattat agacggaaga ctgtggtcgc ccgtcttttt ttgtacccaa aaacaggagg
     6241 aaaacatgaa tcgtgaaagt aaaccaggta tggaaagcgt aaaaatcgga gggttgacat
     6301 actctgttga aaaggcatcg gacctgcaag gcaaagatgg caattggggt cttatacact
     6361 acaaaacaca acaaattaaa ctagatgact cattaaccga gcagctcgaa gatcaaacac
     6421 ttatccacga aattgttcat ggaattttag ttgaggcagg ctatactaat cacgaagagg
     6481 accaagctaa tcgcattggt ctgattttgt atcaagtatt gcaggataat gattttagct
     6541 ggttgtacaa aggggaataa atatgaataa acgcatcaag aaaaaacgta agttagaaac
     6601 agcagttgtg atgcttgttg cagaaaatgc tatacaagca aaagctctta gaaatcaaaa
     6661 caaacaaatt gcagagctga gagcgattat acaacaaaac gtccaagcaa taaatagaga
     6721 gtttgtaact gctaaaaaca cgattttaga taaccaatta gctattaagt taattggtga
     6781 tgatgttggt cacatcaagc aaaattataa gcggaagtgg cgaaaataaa ttttaaactg
     6841 gtcgaattcg acccctttag aatcaaacta agtcgtagga atacggtttt tttattatgt
     6901 ccaagcattg acgacgataa aagctatgga attaataatc ggggacgatt taaaacatag
     6961 gaggtgccaa tcatggcaga agaaacacaa gtagttgaga tggttgaaga gcaagtggta
     7021 cctgaagcaa aacaaccgca agacgaaaaa aagtatacag atgcagatgt ggacgctatc
     7081 atcgacaaaa agtttgcgaa atggaagtca gaacaagaag ctgagaaatc ggaagccaag
     7141 aaaatggcta agatgaacga gaaagagaaa gcagactacg aaaagcagaa gctgttagac
     7201 gaattgcaag aactaaaaaa cgataagaca cgcaatgagt taacagcagt agctcgtcaa
     7261 atgtttgcag aatctgaaat caacgtcaac gatgacgtac ttggtttagt tgtgactttg
     7321 gacgcagaac aaacaaaagc aaatgtaaca acgctagcaa acgcatttgc taaagttatc
     7381 gctgatgacc gcaaggctct tgtacgccag accactccgt caacaggcgg tggattgagg
     7441 aaacaaacca attacggtgc taacttggct agtaaggcag cacaacaaag caccaagctt
     7501 ttttaggagg aaattatgaa caaacgtaaa gtaacaacat ctaaagagat tctacacaat
     7561 ctcgactatg aagctatttc agtaactttg gattcaaaaa aaatcggcaa ggaagttgtt
     7621 ccagctggga cagtgttagc aggtgtttca ggatcagtat ttgaagaccg caaacagaaa
     7681 gttaaagcgg tcgataacag tgccgtagaa agtgaggata acatctgcgg gattttgctt
     7741 acagatgtcg atttaacaaa tggcgatgca gtcggttcct gtatttatcg tgggactatc
     7801 aatgcagaca agcttgctga ttcatctatt gcgggaaaat atgaagtgtt ggaagaagaa
     7861 ctaccgcaca ttgtctttat caagggaggt aaataaacat ggcattgatt cacgaaatta
     7921 tcacatcgga aaatatcaaa ggtttttaca atgctaaaaa cgaaaatgtc ggaaacacgc
     7981 taggggaaaa cgcatttccc ccaaaacaac aattaggact caaactttcg tttattaaag
     8041 gtgcagcagg caaacctgtc acacttaaag cggcagcctt tgatacaaaa gtgccactac
     8101 gtgaccgtat gactgttgaa ttactcgacg aagaaatgcc attcttcaaa gaggctatgg
     8161 ttgtcaagga agcagaccgt caacaactca gtttgttagc tcaaactaaa aatcaagagc
     8221 ttattgacac agcgctagca gcgatttaca acgataaagt aacacttatt gcaggtgcaa
     8281 aagcacgtct agaagcaatg cgtatggaag tattgtctaa aggtaaaatc catgttgcat
     8341 caaacggtgt catgaaagat tttgattatg ggttagattc ttcgcaaaca actaagccag
     8401 atacaaaatg ggaacaagta gataccgcta caccacttaa agacatcgaa aaagctatcg
     8461 aagtaatggc agaacgtggc tttgtgccag aagctatcat catgaactct aagacactta
     8521 gcctgattaa aaatgcaagt aacactttag acgttgtaaa gcctatggcg ccagatgggg
     8581 cagctgttac taaaggtgat ttggaaactt atattgctga tgaattaggt cttaaaattt
     8641 tgcttaagga tggaatgttt gtcggagacg acggtaaagc taaaaaatac ttcccagatg
     8701 gatttgcaac tttagttcct aacggcaatc ttggttacac agtatttggt actactccag
     8761 agcaatcaga tttgcttggt ggtgaggcaa cagatgctga ggtctctatt gttgaaacag
     8821 gtattgcaat taccactact aagacaactg acccagtgaa cgtccaaaca aaagtttcta
     8881 tgattgcttt gccatcgttt gaacgcttgg aagaagtaca aatcattaat gcaacagaat
     8941 ccgaagaaga aactaaaaaa gaaaacagct ttgaaatgta ggaggtcaat atgcctagag
     9001 taattagagc atttaaagat aaagtaacaa aagtagtcta cgaagtcggc gatatttact
     9061 cgggcgaccg agtagagttt ttgacagagg gtggtgtttt agaaccgtcc gtagactttg
     9121 acaagctgaa agtgagtgag attaaaagca aacttgacga gttaagcatt gagtatgatg
     9181 ctaaacttaa aaaatccgag ctattgaagc ttttaaagca aacaatcgga tagtttggag
     9241 gtgtttatgg atgcagtaaa cacaagtagc gttataagca atgtaaagct tgatttaggc
     9301 atcttagaca gtcaacagga cgatttactt aacatgttgc taaaacgcgt tacagaccat
     9361 tttaaagcta aatatggcgc tgtcgaaata gacagcgctt ttagttttgt tttagaagat
     9421 tgtttaattg ctagatttaa ccgtagaggt gccgagaggg caaaaagcga gagtgtggaa
     9481 ggtcatacga caacatacta cgactttttg gatgagtttg aaccttacga tgccatgatt
     9541 atggcaaagc ttaatttaat caaagacaaa tctcgtaaag ggggactgta ctttttatga
     9601 gatatgcaga tagagttaca tttgttaaaa cgacggatga gcaatacaat cccgatttag
     9661 gtgagtatac gcacacagag gtcataagta tcacaaaacc ttgttttgtg atggacatgg
     9721 gcatggaaaa gtccgtacag atttttggag attatcaaaa ggatcgtaaa gttatctacc
     9781 tgaagcagcc ttatacaaaa gcatttgatt attgcgagta tgagggcaag agatacaaag
     9841 cgcaggcaaa taagcttggc gctattgttt tttatctgga aggagatgat tctattggtg
     9901 gctgatatat ctttaaaagt agttggaaca gctggtttaa aaaagaaact tgagcttatt
     9961 gtcaaaaaag atgccgtcaa gaagattgtc agagacaatg ggacgcagct tcaaaggaaa
    10021 atgattaata aagcggtatt tacaaaaggc tattcaacag gtgcaactag acgttctatt
    10081 accatgcaaa tcggcgatgg tggattgagt gtcaaggtta aaccaggaac tcattatgcc
    10141 ggctaccttg aaagaggaac tcgccttatg agcaaacaac cgtttgtttt gccagctcta
    10201 aaagagcaaa aagtaaaatt tagaaaagat ttggaggcgc ttgtcaaatg attaaaacta
    10261 gagatcagtc tatttttgac gaaatgttta aacgcatcca gtctttaggt tttaaggttt
    10321 acgattataa accgatgacg gaagttccat atccatttgt ggaaatggaa tctactgatg
    10381 cggaatatat tccaaataaa gatgacatta aaggttctgt tgaacttatg ttgtctgttt
    10441 ggggggtgca gaaaaaacgg aagcaggtgt ctgacatggc atctgctatt ttttcgcaag
    10501 ctatgtccgt agctcgtacc gatggattct gttggtcgtt taatattagg cagtcgtcta
    10561 tacagatgtt agatgataca acaactgtga cacctcttaa acgagcgatt gtcacactta
    10621 gatttaattt gagataggag gtagtaatgt cagaacctag caaagcaaca acacaggagg
    10681 agaaagaagt aatgtcaaaa gcacaagaac aaacaaaaca attagaagca aaacaaggaa
    10741 ttcattcaat cttgttattt cgtttgttga aagaagcatc tagtgaggta gcaactaaac
    10801 ttgcttttca aacagagcat gaagtaggta aaagccgtga cgtagatgga caaaaaacta
    10861 aagatggtat tatccagtcc gtgggggctt tggagtacga ctttaaagca acatctattt
    10921 tagccaaagg tgacgaacta gcagctaaac tagaaaaagc catggaggat ggtgagcttg
    10981 tagaaatttg ggatattgat tcagaagaaa taagcaaaaa cggcgacagc gacaataagc
    11041 ttgcaaaagt ttggggtatt aataaaaatg gaacaaacgg aggaaacggt aaatgcctag
    11101 cgacttatta ccaagggtac atttcaagct tcagcgctaa gaaaaacgca gaagaaaata
    11161 ttgtaattga aatggagttt gccatcaatg gtgttggtaa aaaagggttc gctacattaa
    11221 cagatgccca aaaagcagct gtacagtacg catttaaaga tacaacaaaa tccaacagcg
    11281 aagaaactaa aaaagaaaac agctttgaga tgtaacggtt aggttggatt tagtatccaa
    11341 ccttttattt ttaaaggaga aaagaataat gcaattggaa attaaaggta aaactcataa
    11401 cgtaaaattt ggtacacgat ttgttgccga gatggacaaa aatcatatag cagaacgtca
    11461 gggatttaaa tttggagctg gtctacaatc tagcgttccg tttttaatcg accacagcgt
    11521 tgttactctt gcggaagtaa tttatacagg tacaatcacc gaaccaccac gtcctagttt
    11581 gaatgatatt tacgactata ttgacgaagt tgaagatatt gaaaagcttt ttgatgatgt
    11641 tttagacgaa ctacgtcaat caaacgcatc aaagttgttt atggctcagg tggaaaaaga
    11701 cctggcggaa gccaaagcgt aacaaatata aaaaagagtt attcttctca agaaagcttt
    11761 gagacgattg tgcttaattg cattagatac cttggcatga ctgacatcaa tgaaatcggg
    11821 cgattaactt tgtatgaata tgatttatta atgacaggca aagcactagc ggctgttgat
    11881 gaatcacata aagctcacaa acaagcttgg ataaaccacc aagttacggc aacaaagctt
    11941 gttggtggca agaaaaataa aaaagaagtc cctgtttata aaaaattcaa ggactttttt
    12001 gattatgagg aagaaatccg aaaaatcact caagaaattg atgaaggtta cgacaagaaa
    12061 ggtatggatt tacttctcaa agctaacctt taaggaaagg aggttaaatg ggagaatctt
    12121 attctgttga agcggttttg acagctgttg ataaaacctt tggcaaaaca ttacaatcgg
    12181 caatccgttc aatcgaaggc ttggaaaagc gttcaaccgg tttttcatcg gtgtctcaaa
    12241 aagctagttc catgtttaaa tccatgttag gagcgaattt agccggacaa gctatctcgg
    12301 caatgacaag gacagtgtca tcaggccttg gctctatgct tggcgagatg aatagttcag
    12361 cgaaagcgtg gaaaactttt gacgccaatt tagcggacat tggatttgga aaaaaacaaa
    12421 ttttggcagc taaaacggcg atgcaagact atgcaactaa aacaatctac tcggcatcag
    12481 atatggctag cacgtatgca cagttagcag cggttggcgt gaaagatacc ggaaagctcg
    12541 taaaagcttt tggcggttta gctgcatctg ctgaaaatcc gaagcaggcc atgaagtcta
    12601 tcagtcaaca aatgacgcaa gcagtaggaa gaccaacagt tgcatggcaa gactttagga
    12661 taatgctgga acaggcgcct gcagggatgg ctaaagtcgc taaatctatg ggtaaaaatc
    12721 ttgatgaact cgtcgccgat atccaggcgg gtagggttaa aaccagcgat tttttggaag
    12781 cggtaaaaaa agcaggcaat gataagagtt tccaaaagat ggcaactgag ttcaaaactg
    12841 ttgaccaagc catcgacggt atgcgagaag gcttatccaa caaattgcaa ccagcgtttg
    12901 aaaaagtgaa ccaatttgga attagagcga tcgaagcaat cggtaaacaa ctcgataaag
    12961 ttgatttttc taagtttgct agtaatcttg ggaaattcct tgaaggaatt aatatcgata
    13021 aaattgtatc taatatttca tcggcgattt catctgtcac ttcaaaggtt aaagaatttt
    13081 gggacggttt caaacaaact ggagcaatta gtgctttttc aggagctttg cagagcgttt
    13141 gggaagcttt aaaaaatgtc gctagcgcca tgagcggagg aaattggaag acttttggag
    13201 caacagttgg agggactgtt aaacacgtct ctaacttcgc taaagctgtt tccgatgttt
    13261 taggaaagat ggaccctggc agactaagaa gttggatagc taccttcgcc gcagtagctg
    13321 gaggctttaa attattcgaa aaattaacgg ggcaaagcgt cattggttct tttttggata
    13381 aaattggcag caaatttggc ctctttggaa acaaagccaa agaaggaaca gacaaagcct
    13441 ctaacggcgc tagaagaagc ggtggcatta ttagccaaat cttcagcggc ttgggtaata
    13501 tcgttaagtc tgctggtaca gccatatcaa cagctgcaaa aggtatcgga gctggtatta
    13561 aaactgcttt gtctggaatc ccccctatca ttagttctct aggaaccgca atatcaacag
    13621 ttgcgcaagg tataggcact gggctagcaa tcgcatttaa aggccttggt tctgctattg
    13681 ccatggtgcc gcctaccaca tggctagctt taggagcggc aattttaatg gttggtgctg
    13741 cttttgcctt ggcaggaact caagctgatg gcattagtca aattttaaga accgttggcg
    13801 atgttgttgt gcaaatctta caacaggtca ctgatagtct agccacttta ctacctatta
    13861 tcgcaaacgc tattggctct atgttgccaa ttgtagctgg agctatctct cagattgtag
    13921 gcgcagtagc gggcggatta tctcagctcg ttatagccgt ttcaacaggg gcatctctcg
    13981 ttataggggc tttcacagga cttcttggtg gtatttctgg ggttattaac tccattagcg
    14041 ctgttatcca atcgctaact ggtgtgatta ccgcagtatt caatggcata gctactgtta
    14101 tttcatctgt cggttcggct atcaaagatg tattgacggg tctaggaacc gcttttgaag
    14161 gatttggaaa tggtgtaaaa tcagctctag aaggtgttgg ggcagtaatt gaatcgtttg
    14221 gtagtgcagt taggaatgtc cttgacggtg ttgcaaatat ccttgattct atgggtactg
    14281 cggcacttaa tgcaggccgt ggcgtaaaag agatggctaa aggcattaaa atgcttgttg
    14341 atttatccct tggagatttg gttgctacat tagcagctgt ggcaagcggt ctagggaaga
    14401 tggctagctc agctggcgaa atgacaacat taggttctgc tatgagcaag gtagccaatg
    14461 gtatgacacg tctagcaaca agtgctacga tagcaattac tggattaaca gtctttgcca
    14521 ccaccatggc aactattaag acagcagttg caactctacc gccagtccta acgatggcag
    14581 cgagtgggtt taccacattt actactcagg cggtggcagc agtgactgga ttggctgcaa
    14641 ttaatgctcc aatcactatg tttaaagctc aactaatgac aataacacca gctctagcac
    14701 aagctggcgc tggctttgcc gcgtttgttg ctcaatcatc aacatttagt acaggtttag
    14761 catctgccgg tcctacaata gcagcgttca atgctaattt gatgagctta tctgcaacaa
    14821 caggagcgct agttgcatca atagctggtt tatcagctgt gctttctgtt gtatcagctg
    14881 gctttagcca aataggggct tctgcgacag caactgttgg tcaaatacaa gcttttgctt
    14941 ctagtacaac agttgtttcg tcagcatttg ctagcatgca atctatgatt caatctgcca
    15001 tggctgcaat agtaagcagc attataacat catttaatca agcggcctct caaatgcaat
    15061 caatcttatc tcgaatgcta tctcaggcta ggacatttgg gtctcaacta gagcaacaaa
    15121 tgagacaatc gggacagcgt tcaggacaaa atcttgctcg gggactatct tctcaacaaa
    15181 gtgctgttat taatgctatt tctagcatgg ttaatgctgc ggtatcaaga gccaacgcgg
    15241 gagctggtcc tatgcgtcaa gctggagcgt acatcggaca agggcttgcg caaggaatgt
    15301 attcagcgct aggagctgta acagctgcag caaacgccct tgtagcacaa gccgagagag
    15361 cagcaagagc caaggcgatg attcattcgc cgtcaaggtt gtttgcaaaa cgagttggtc
    15421 aatatatccc gcaaggggta gctatgggta tcgacaaaaa cgctgatgtc gttgacgact
    15481 ctgttggcgg gttatttgat agcatcaata gctttgattt taatatcgca gatagactgg
    15541 ctagcattgg agctaaattc caaggtgtta tcaaatcaga gagttcacaa tcgttatcgc
    15601 agcaacaaga gttcgtacat acagctcaac cagcgtatat aaactttagt ttaggtggaa
    15661 acgaatacga agcatttgta agtgacatca ctaatcaaca agcaaaaatt gaaaaaatca
    15721 gactaaagag aagcagctgg tagttgtttc tcttagtttt ttgaaaggag taaaatgtac
    15781 gaatttaacg atactatcag aggtactccg aaagttactt ttaatttaaa gacaacaatt
    15841 ggtgaaaagg tattagaaga agaactcaat aatgatttag gtacctttag gacattgact
    15901 gtttctggtc gtgatgtggt ggacttagag catcaaacaa caagtgtttt agggcgaaat
    15961 ggtgagtatt tccacaatgc cacagtcgaa gtcagaaaac tagaaataaa agctaaaatc
    16021 actggaaaag ataaccagtc aatgcgtttg caatatgaaa aattaaacag attaattgtt
    16081 agtcacaatc aagttttttt atcattttca gacgaacctg acagaaatta tctaggcatc
    16141 tttaaatcta aagatgtccc agaagaagtt tctaacgagc agattatagg actaacattc
    16201 atctgctaca atccgtttaa aatgtctgat gtaaaaacta aaaaaggaac atctatccaa
    16261 aatggtgggt tatttcaaac aaagcctatc atcactctca acctatcatc accaacaaaa
    16321 gaaattaaat tgttgcatgt cgaaagccag aagtatatca gattgactgg aacttatacc
    16381 actgacgaaa tcaagattga tatggccacg ggtaaaatta cccagaacgg tcgcaacatc
    16441 cttggagatt tagatatgat taatagtagg tattttgagc tattacctgg taataataca
    16501 ttacagtgtg ccaatgccgc cataaccgca gagtttagag aggtttatct atgatatacc
    16561 tgtttgataa acttgagcga ttggtcgcta ctgttggtac tgatgacttg ctctcatggc
    16621 actttaaggt caaaaacaat gattgggacc aggctagttt tgaagtgcca gttgattatg
    16681 acgtagagcc atttgtctac tttggttttt ttaactacga ccctaatcaa aaagaagatg
    16741 tctttaagct ctttaaagtc attgattaca acctagaaga tagcaagttt tacaaaggct
    16801 tagacaaagc tgagagtgat cttgatacca ttgccattat caaagataag cgctttagac
    16861 aatcgtccgc agatgcttgt attgatggtg ctttagaggg tacgggttat caagttggca
    16921 aagttgaggg aataacaaat gttagaacac ttagttatta ctacatcagt ccacgagcgg
    16981 ctctgattaa gattgtggaa gcttttaact gcgagttcaa tgtcaggtat acctttatta
    17041 ataacaaaat cactagtcgt tatatcgacc tcaaaaagcg ctttggaaag ccaacaggca
    17101 agcaatttga acatggcaac aacctgttaa aagtcgtcta cgaggaatca acagatgaca
    17161 ttgtgacttg tctgattggc agaggtaagg gtgaggaaat ccaacacgaa gaagctgagc
    17221 ctaaagatgt cgagggacac ttgccacagg aagaaaggcg gcaaggctac ggtcgaagaa
    17281 tcgaatttac tgatgttgtc tggtcggtcg aaaaaggcga cccgatagac aaaccagctg
    17341 gtcaaaactt tgtagcacta gatagtgcaa gggaagaata cggcttgtct caaaatggtg
    17401 agctaaaaca ccgctggggt gtctttgtca atgaggaaat cgaggataag acagaactct
    17461 taaaagcaac ttgggaagaa ttgcagcgtt tgtcaatccc tatcagaatt tacaaggcag
    17521 aaatcttaga cattggtcca gagacgtgga aaggcgactc agtagccatt atctatgatg
    17581 aggtaaaaat agcttttgaa actcgggttg atgagattga tattgacaag cttaatttta
    17641 acaggtcagt cgtaacactt ggtgattaca gcgttgtcca aaatcgtgag tcacggtctc
    17701 gtaaagaggc tgtccaaaac atgatagatg aatctttaga gactatcaca gacttaggaa
    17761 tgacatttca ggaatttttg caaggcatcg aaaaacgcat cgagactggc aaaaaggaga
    17821 tggaagacaa ttggcgaaaa gttaaccttg aattcgataa ctttaaaaag aaggttgagc
    17881 aagaaggctt gcaattcaac accttgaaag aacaaatcaa agaagttgat gaacgcaccg
    17941 acaaagagct agaggaattc cgagccaccc tcaaaaacct agcgttacca gaggaagcga
    18001 ttaaaaaaat cacagaggct atcaaagttg atgacatccc gtctcttaag caaaactttg
    18061 atgacttaaa aaacaaggtc agcgagacaa gcgagacggc acgtctaaat gccgagatta
    18121 tcggtacaga cggtaagacc cgctataaca aaaatttatt ggttggcgac cctaaccgtg
    18181 taaaaaccta tgaccaagac tatatcgagg tagaagctaa tgatggcggc tttaagcgtg
    18241 gcgagaccta cacgattagc tttagtcaaa cgtgtgagct actcaaaaaa gtagctatca
    18301 cattgacgca aattaacaac aagggagtta agttagtact gacaccaacc aaagcaaaaa
    18361 tggatgcaca gacgtttgag gtcactaaag ataaacagtc tatagaggtt tatcctttga
    18421 gctatacagg cgttttaaca ggtgattggt ataaatctaa gtctgtggag ataaacgcct
    18481 cagaggcgca ggaattagct cttgagatgg cttataaaga gattgcagat gccaaaggtg
    18541 caactattat cggcaaacag tccgataaac caaaaattat tttagatggg aggagggaca
    18601 gatgacatca gtcgaaacaa taccaataaa aattgtcttt gaccgaaaag acgcttcaga
    18661 atggcagtca actaaccctg tcattgacga aggtgaacta gtcgtcgagc tagacaccca
    18721 taagctaaag gtcggagatg gtaaaacaag ctacaatgac ttgccttact atgaaggtcc
    18781 gcaaggagaa tccataacaa aagtacaact atccgaaaat ggtgacttgt ctgtgtggat
    18841 tggcgacaaa gagactaagc ttggcaacat caaaggtcaa aaaggggaca aagggacaag
    18901 tataaccgac atcaccaaag atggtgagac actcactatc aaactatcag acgacactca
    18961 aaaaatcttt aatatcccca atggccaaaa aggggataga ggtaagggcg tagagagcgc
    19021 taggattgac gaatccggtc atttaaaatt aaaaattgaa gaggaatcag agttagatct
    19081 tggaaacgtt aaaggtgagt caggacctaa aggtgatagt attactatca caaatcacaa
    19141 gcgtgtttca gatggaacgc aggtatcttt cagcgacggg acacaaattg ttgtcccaaa
    19201 aggggatact ggtgatgtca acggcatcaa tctggaagat tatgtcaaaa aatctgaact
    19261 taaaaacgtc ggttctgcag atgttaaagc tattaatgac tttttagggc tatctcaaaa
    19321 ggtgtttaca agcagctata gttatacaga tagcttacta aaaagttatg caaaacccag
    19381 ctattcggcg agttggtatg tcaacgaatc tacagtttct accaaaaatg gtgacaaagt
    19441 attgattaca atacataaca ctaccaccca agcagacaat tatttggagg tagcggtgac
    19501 ttatgttggt gctaactacg tgactgctac ctcaacaggt cgcttactga ctactcctgg
    19561 tgaagtcaaa gtagtgacaa aaaagcaagc ggaaaaagat tatgctgcta aaaaacataa
    19621 gcatgagatt agtgacatag ctggtcttaa tgagcgcttg tctggctatc tcagacaagc
    19681 tgatatacag tcacagctta ataatatcgg taagctaaaa gacacgcaaa ccggacaata
    19741 tcttgaggtt aaagtggttg ataaaggaca ggtgcctagc aataccagtg gcatgatcgt
    19801 gtttgagagg tcataatttg ttaaaagatt taaataatat cattattaat aacaaaacga
    19861 ttgatcgtat tatttgtaat aatacgattg tctatcagcg ttcgtgggat ctgctttttg
    19921 acggtttgct gacaagcgct gggaaaaaat tggatattta tcctttttat aaaatcgtaa
    19981 tcaataatgc attttttgtt aaaaccccca caacaaaaaa caatacgatt tttttagcag
    20041 ggcataaaat actagcatta caagatagtc atttttgttt ttttagagca ttagaaaaaa
    20101 gtttttttgt caaaatttac ggtaaaaatt aggaggtaac atttgagtag agacccaaca
    20161 cttttaatag acgagtcaaa tttaacaatc ggctcagatg gacgtgctta ttatacattt
    20221 acggctgatg gtgacacaaa aagcgttaaa atagccaata acaaatgtat cggtacaact
    20281 cgctttaacc agctcatgat tgagcgaggg ggtaaaccaa ctaactacgt ggcgcccgtg
    20341 gttgtcgagg ggacaggtaa tccgactgga ctatttaaag acctcaaaga gattagcctc
    20401 gagttaacag atactaaaaa ctccaaactt tggtcaaaaa tcaagcttaa taatcaaggg
    20461 atgattgaag agtattacaa cggtacaata aaatctgaga ttatcaaaac cgcagagggc
    20521 acgcagcaac gtattagtag cgagaccgat aaaaaacttg cgcttatcaa cgagacagtc
    20581 tcaggcatta gacgtgagta ccaagatgca gatagacagc tatcgtcaag ctaccaagct
    20641 ggtattgagg ggctaaaagc cacaatggcc aatgataaaa tcggtttaca agctgagata
    20701 caaacaaccg ctcaaggctt gtatcaaagg tatgataacg agatacgcaa gctgtccgct
    20761 aaaataacca ctacctcgtc aggcactaca gaggcctatg aaagcaagct cgatggctta
    20821 cgagctgagt ttactcatag taatcaaggt atgcgcgtag agctggagtc aaaaatcagt
    20881 gggttgcaat caacgcaaca agcaactgcc aggcaaatct cacaagagat aagtaaccgt
    20941 gaaggtgctg tcagtcgtgt acaacagggc ctagacagtt atcagcggcg attacaaaat
    21001 gctgaaggga gttacagcag cttacaacaa actgttagtg gtttacagtc tgatgttaat
    21061 agtccaaaca gtaagcttaa ttctcggatt agccaattag ctagtcaaat tgaccaacga
    21121 gtcacaagag ccgatgtgac aagcatcatt aaccagtctg gggacagtat caagttagcc
    21181 atacagagag ctggtgggat tgatgctaag atgtccgcaa aagagattgt ctcagccata
    21241 aacctcaatg gctacggtgt cagaatttct ggtgagcgta ttgctttaga cggcaatacc
    21301 acggttaacg gggcttttgg cgcaaaaatt ggtgagttta tcaagctaaa agccgaccag
    21361 attatcggtg ggacaatcga tgcaaacaaa atcaatgtga ttaatctcaa cgctagcagt
    21421 attgttggtt tagacgctaa ttttattaaa gcaaggattg agtatgcgat cacaagtttg
    21481 cttgagggta aagttattaa ggctcgtaat ggtgcgatga caatagactt gcaaagcggt
    21541 caaatcaacc attacacaaa tgagtcagcc atgagacgta tcgatagcag tacagctagt
    21601 cagtttataa agatgacaaa gtcaggcttt atatctgaga taggtaacat gcaagctgca
    21661 atgacggtta taggctctaa tagcgatggg tcagaaaacc atgaaaataa aaccttcgga
    21721 ggcataagaa tttggaacgg gaagtcatct tatcaatcga caagttttgt tgaattagtc
    21781 ggtaaccgtg tagcaatcta tggaaataaa aatcgtagtc catggctctt tgactcaaca
    21841 acgtcaggat atgcttacct cattccacag aatgaaagtg gtataaagca tgccataggg
    21901 agagctgatc gtaagataga ccaaatccac gttggagata tctatgtgca aggcgaacgt
    21961 gtcgctatga tgttaaaaga tttagcgaat aggattggct atataggtac aggtggctgg
    22021 gctaaccgca taggataaat taggagaaac aatgcaagaa aaattattag gaaaaattat
    22081 taacgattta acacttaaag tcgctaattt aacgctggaa aatgctcaat taaaagcaca
    22141 gcatgaaatc gaattggaag aattaaacgc acaattggat gaagcaacag caccgaagga
    22201 agaaggtaaa taaacatgag aaattggaaa gtaacaggaa actacccaca gtgggacggt
    22261 acaggagcag tcattgatac gcaggtaatt attacagatg acaggggtgc agtcatcgca
    22321 gagaagatca aaaaagattt gagaacagct aatgatgcag aaattatcga ggctgcttta
    22381 gaggagttta aaaagactgc ttacgtcgaa atcgctatgg gcgaagccgt gcaaaaagta
    22441 gacgaccttg aaaaaatctc acaggaaact gctaagactg ccaaaactgc tcaaacagct
    22501 gctggactag ctaaggtgtc cgcagagcgc acgcaaaaga tgattaactt acaaacgatc
    22561 cacatgttaa cgagcggcgg caagattgat cctgacattt ataaaggcat gcttgagcta
    22621 atcgagccag ccaaaaaagg tgagtatcaa gcgtatgatg tctttacggt ggtcgacagt
    22681 actaaagagg aagacggtga agcaggcgaa gggaacctag tcttcgtaca cgttaacgag
    22741 ccgtttactt atgaggcgca aactcttaaa gatttggaat cagaggataa agtcacagtc
    22801 attaaatatg cggatttggt taaacaagat tagaggtggt tagatgatag attttgtaca
    22861 aattggtgct ttttgtggcg ctgctttgtc tatcttgggt gtttggggat ttatcgtcaa
    22921 tccctttaaa aaagccatgg aagctaatga gtttgccatg gctcaactca aggactcaat
    22981 taaggagtta gcttatgagc ttaaaaatct tgatcgtgaa cgtgagatta ccaaaaaaat
    23041 tatcgacaga cacgaggagc gtttaggtcg cgtagaagac gaagttatta tcaacaagga
    23101 gcgtattatt acgctattta aaaaaggaga agaaaaatga ataactggtt taaaaaagta
    23161 gcaattaaaa caattaaaac aatggcacaa acggccgttg gtcttatcgg gtcaagcatg
    23221 ttgattacgg atattaactg gccaacaatg ttgtcagcag tgctgttgtc aggactaaca
    23281 tgtatcctga tgaatgtgtc acaaatcaag gaagaggaat aggtcatgcg agcaatcaca
    23341 cgattagcat tagttatagc aatcgcaata ctgtatgtgc cattatctgt ggttgctttg
    23401 attttttatc catttttaga taaggaggga taaatggcta catatcagga atataaaagt
    23461 cgttcaaatg gcaatgctta cgatattgat ggatcgcttg gtgcgcaatg ttgggatggc
    23521 tacgcagact actgtaagta tctaggactg ccatacgcaa actgtacaaa tacaggatat
    23581 gcaagggata tatgggaaca acgtcacaaa aatggtattt taaactactt tgacgaagta
    23641 gagactatgc aagctggaga tgtcgcaatt tttatggtag ttgcaggtgt tacaccgtat
    23701 agccatgtgg ctatttttga tagtgatgca ggtagtggat acggatggtt tttaggtcaa
    23761 aaccagggtg gagcaaacgg agcatataat ttagtaaaaa taccatattc aacgacttat
    23821 cctactgcat ttagaccaaa agtttttaaa aatgcagtta ctgttatagg taatatagga
    23881 ctaaataaag gcgattattt tattgatgta tcagcttatc aacaagcaga cttaaccgct
    23941 acttgtcagc aagctggcac tactaaaacg attatcaaag tatccgagtc actcgcttgg
    24001 ctgtctgata ggcatcagca acaagctaat actagtgacc ctattggtta ttatcacttt
    24061 ggacgatttg gaggagatag caacttagcg caacgagaag cagatttatt tctgtccaat
    24121 ttaccaacca aaaaagtctc ttacttagtc attgattatg aagactctgc aagtgccgac
    24181 aaagaagcta acactaatgc agttattgcg tttatggata aaattgcaaa cgctggatat
    24241 aagcctgttt attacagcta taaaccattt acgcttaata atattgatta tcagcaaatt
    24301 atcgctaagt acccaaacag catttggata gctggttatc cagactacga agtacgaaca
    24361 gagccacttt gggagttctt cccttcaatg gatggtgtgc gctggtggca gttcacaagt
    24421 gtaggagtag caggtggttt agataaaaat attgtattat tagcagatga tagtagcaaa
    24481 gtggatatac ctaagattga caaaccacaa agccagctta cttttaatca aaagctagat
    24541 actaatacta aattagacaa ctcgaatgta ccttactacg aagcaaccct tagaacagac
    24601 tattatgtag agtctaagcc aaacgcaagt agcgctgata aagaatttat caaggcagga
    24661 actcgtgtaa gagtctatga aaaagtgaat ggatggtcac gtattaacgc ttctcagtct
    24721 gaccagtggg tcgaagataa gtatttatct aatgcaacac aagtataaaa taggaggtaa
    24781 agctccttta gataagacaa aaccgctcag ataatttctg ggcggttttt tgtgtatgat
    24841 gaattatttt tcaagataaa tatcgaaatg actaaagttc ttcatattga taattctatt
    24901 atctttatat tttgcaaaaa tatctgatct agtcccttca tttggtgagt caaataagtc
    24961 tatttgctca tgtttcccat cttttgtgcc aatttcgatt ctgccgctta cataaggaga
    25021 agtagcgtca taaattttat aattatccat aaggtatttt ctgattttaa agtcaatttc
    25081 ctggaaagtt acgatatctt tttctagaat aattttgtta tttaagttct gttgagattc
    25141 tcccgaaata aatagatttc ccaataattt atgatttact ttattatttt gagcaggcgt
    25201 aattcctcca tagatgtact caccggtgtg agaattaaga atataaaata atccaaaaac
    25261 atctacatga tcatctcgtt taaatttttg agaggcctca taagacattt cggaactaat
    25321 ataatagtct ttccctctat atttttgagt atcaatgttt aatgtgtgtg tcgttgaaaa
    25381 atttaccctg caatctttat aatcataagg agttatagtg tatgcataaa gtaaatcact
    25441 tttaacattc gaaatgtctt tcttagagtc acttttgatg ataggtgaaa tagtagaaat
    25501 cagtatgact gtaattatga aa
//
LOCUS       DATXIG010000019        24475 bp    DNA     linear   BCT 28-DEC-2023
DEFINITION  TPA_asm: Streptococcus pyogenes strain MGAS11113
            SAMN08555799-rid19682583.denovo.019, whole genome shotgun sequence.
ACCESSION   DATXIG010000019 DATXIG010000000
VERSION     DATXIG010000019.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN08555799
            Sequence Read Archive: SRR6740495
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pyogenes
  ORGANISM  Streptococcus pyogenes
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 24475)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 24475)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-OCT-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-OCT-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001918058.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001918058.2
            Genome Coverage       :: 368x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/12/2023 11:23:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 1,806
            CDSs (total)                      :: 1,769
            Genes (coding)                    :: 1,722
            CDSs (with protein)               :: 1,722
            Genes (RNA)                       :: 37
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1, 1 (5S, 23S)
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 47
            CDSs (without protein)            :: 47
            Pseudo Genes (ambiguous residues) :: 0 of 47
            Pseudo Genes (frameshifted)       :: 30 of 47
            Pseudo Genes (incomplete)         :: 20 of 47
            Pseudo Genes (internal stop)      :: 9 of 47
            Pseudo Genes (multiple problems)  :: 11 of 47
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..24475
                     /organism="Streptococcus pyogenes"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN08555799-rid19682583.denovo.019"
                     /strain="MGAS11113"
                     /serotype="Emm28"
                     /isolation_source="normally sterile site"
                     /host="Homo sapiens"
                     /db_xref="taxon:1314"
                     /country="Finland"
                     /collection_date="2002"
                     /collected_by="National Public Health Laboratory"
     gene            100..333
                     /locus_tag="VC122_000846"
                     /pseudo
     CDS             100..333
                     /locus_tag="VC122_000846"
                     /inference="COORDINATES: protein motif:HMM:NF012870.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DDE-type integrase/transposase/recombinase"
     gene            361..588
                     /locus_tag="VC122_000847"
                     /pseudo
     CDS             361..588
                     /locus_tag="VC122_000847"
                     /inference="COORDINATES: protein motif:HMM:NF025063.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
     gene            715..870
                     /locus_tag="VC122_000848"
                     /pseudo
     CDS             715..870
                     /locus_tag="VC122_000848"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003039767.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ISL3 family transposase"
     gene            complement(917..1447)
                     /locus_tag="VC122_000849"
     CDS             complement(917..1447)
                     /locus_tag="VC122_000849"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002987925.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ParB-like nuclease domain-containing protein"
                     /protein_id="HEP5640786.1"
                     /translation="MSNFISPVYEIKSIPIEKISPNDYNPNSVAPPEMKLLYDSIKSD
                     GYTMPIVCYYDKEEDRYSIVDGFHRYRIMLDYSDIYERESGRLPVSVIDKSLDYRMAS
                     TIRHNRARGSHDVDLMSQIVKDLHECGRSDNWIAKHLGMDKDEILRLKQITGLASLFK
                     DHEFNQSWEPEELLNE"
     gene            complement(1440..2774)
                     /locus_tag="VC122_000850"
     CDS             complement(1440..2774)
                     /locus_tag="VC122_000850"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014407264.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoadenosine phosphosulfate reductase"
                     /protein_id="HEP5640787.1"
                     /translation="MKKYLNQNVYDALIERLHFLFNEFPIVYISFSGGKDSGLLLNIL
                     LDFRDKYYPDREIGVFHQDFEAQYSLTTKYVQETFTSLEGRKKVSLYWVCLPMATRTA
                     LSSYEMFWYPWDDKTEDIWVRPMPSQDYVINLENNSITTYRYKMNQEDLAKQFGRWYK
                     QIHGNQKTVCILGNRASESLHRYSGFINKKYGYQKECWITKQFKDVWTASPLYDWSVE
                     DIWHAYYKFSYSYNELYDLFYKAGLKPSQMRVASPFQDYAVDSLNLYRIIDQETWVKL
                     LGRVQGVNFSNIYGRTKAMGYKSIALPKGHSWKSYTQFLLSTLPVRLRNNYVRKFNKS
                     IDFWHKTGGGLAEETINELIEKGYRIARNGISNYTSFKHSRVIFLDQIPDDTDDIVTT
                     KDIPSWKRMCFCILKNDHICRTMGFGLTREQQKIVAFLKEKYKTSLKELESE"
     gene            complement(2996..3295)
                     /locus_tag="VC122_000851"
     CDS             complement(2996..3295)
                     /locus_tag="VC122_000851"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002987921.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEP5640788.1"
                     /translation="MFLTWENLFLTNQSRRRLKLLVVERSYNNVGNFLKEYFGGFFEI
                     INFDDLRLTMVDMVSLSSEYDVIVTDMILEQTMDSEILFFNQMAPSVVANRLTDM"
     gene            complement(3452..3919)
                     /gene="perR"
                     /locus_tag="VC122_000852"
     CDS             complement(3452..3919)
                     /gene="perR"
                     /locus_tag="VC122_000852"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002987920.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peroxide-responsive transcriptional repressor
                     PerR"
                     /protein_id="HEP5640789.1"
                     /translation="MDIHSHQQALDAYENVLEHLREKHIRITETRKAIISYMIQSTEH
                     PSADKIYRDLQPNFPNMSLATVYNNLKVLVDEGFVSELKISNDLTTYYDFMGHQHVNV
                     VCEICGKIADFMDVDVMDIAKEAHEQTGYKVTRIPVIAYGICPDCQAKDQSDF"
     gene            complement(3971..4426)
                     /locus_tag="VC122_000853"
     CDS             complement(3971..4426)
                     /locus_tag="VC122_000853"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002987917.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CoA-binding protein"
                     /protein_id="HEP5640790.1"
                     /translation="MIYSFQNPSEDILKAYLESAKTIAVVGLSDRKDTAAYGVAKFMQ
                     AMDYRIIPVNPKLAGQLILGEKVYASIKAIPFEVDIVDVFRRSEFLPEVARDFLAGQA
                     KVFWAQLGLENQEVQTILRSAGKEAIVMNRCLKIDYLQLVAKPECITNL"
     gene            complement(4613..7255)
                     /gene="polA"
                     /locus_tag="VC122_000854"
     CDS             complement(4613..7255)
                     /gene="polA"
                     /locus_tag="VC122_000854"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011054150.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase I"
                     /protein_id="HEP5640791.1"
                     /translation="MENKNKLLLIDGSSVAFRAFFALYNQIDRFKNHSGLHTNAIYGF
                     HLMLDHMMKRVQPTHVLVAFDAGKTTFRTEMYADYKAGRAKTPEEFREQFPYIREMLT
                     ALGIAYYELEHYEADDIIGTLDKMAERTEVPFDVTIVSGDKDLIQLTDENTVVEISKK
                     GVAEFEEFTPAYLMEKMGLTPNQFIDLKALMGDKSDNIPGVTKIGEKTGLKLLHEFGS
                     LEGIYEHIDGFKASKMKENLINDRDQAFLSKTLATINTASPITIGLDDIVYNGPDVAS
                     LSQFYDEMDFVQLKKGLASQMPQEPVAAISYQEVTNVSADLFSAEDIFYFETLRDNYH
                     CEAIIGFAWGHGEQIYASTDLSLLATDSFKQVFQKPIATYDFKRSKVLLSHLGIELVA
                     PSYDARLANYLLSTVEDNELSTIARIFTDISLEEDDTVYGKGAKRAVPDKDVLLEHLA
                     RKVKVLLDSKSQMLDKLTAHEQLDLYQNIELPLANVLAKMEIEGIKVNRATLQDMAEQ
                     NKVIIEALTQEIYDMAGQEFNINSPKQLGSILFEKMQLPLEMTKKTKTGYSTAVDVLE
                     RLAPIAPIVAKILDYRQITKLQSTYVIGLQDYILADGKIHTRYVQDLTQTGRLSSVDP
                     NLQNIPIRLEQGRLIRKAFTPSHEDAVLLSSDYSQIELRVLAHISGDEHLIAAFNEGA
                     DIHTSTAMRVFGIDKAADVTANDRRNAKAVNFGIVYGISDFGLSNNLGITRKQAKSYI
                     DTYFERYPGIKAYMENVVREAKDKGYVETLFKRRRELPDINSRNFNVRSFAERTAINS
                     PIQGSAADILKIAMINLDKALQAGGFRAKMLLQVHDEIVLEVPNDELTAIKKLVKDTM
                     EAAVDLAVPLCVDESTGQSWYEAK"
     gene            complement(7386..9113)
                     /locus_tag="VC122_000855"
     CDS             complement(7386..9113)
                     /locus_tag="VC122_000855"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003060437.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease/substrate binding
                     protein"
                     /protein_id="HEP5640792.1"
                     /translation="METILQTKLPVAQLVEQLTEWLTKTFSGLFDIMQVVGSFLMDWM
                     TKTLLFIHPLLFIVLVTAGVFFLAKKKWPLPTFTLLGLLFIYNQGLWKQLMNTFTLVL
                     VASLISVLIGIPLGIWMAKNATVRQIVNPILDFMQTMPAFVYLIPAVAFFGIGMVPGV
                     FASVIFALPPTVRFTNLAIRDIPTELIEASDAFGSTGKQKLFKVELPLAKNTIMAGVN
                     QTMMLALSMVVTGSMIGAPGLGREVLSALQHADVGSGFVSGLALVILAIVLDRMTQLF
                     NSKPQEKAKAGKTNKWIGLAALAVFLIAALGRGIMAMTSGMADKGETVNIAYVQWDSE
                     VASTHVIAEVLKNEGYHVTLTPLDNAVMWQTVANGNADFSTSAWLPVTHGQQYQKYKS
                     KLDDLGPNLKGTKLGLAVPKYMTDVNSIEDLSKQADQKITGIEPGAGIMAAAQKTLKE
                     YHNLSSWELVAASTGAMTTSLDQAIKKKDPIVVTAWSPHWMFAKYDLKYLKDPKETFG
                     STENINTIARKGLKKDLPNVYKIIDKFHWTQKDMEAVMLDINKGMSPEAAAKKWVEAN
                     QSKVSSWTK"
     gene            complement(9129..10325)
                     /locus_tag="VC122_000856"
     CDS             complement(9129..10325)
                     /locus_tag="VC122_000856"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014407259.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine betaine/L-proline ABC transporter
                     ATP-binding protein"
                     /protein_id="HEP5640793.1"
                     /translation="METILEVKHLSKIFGKKQKAALEMVKTGKNKSEIFKKTGATVGV
                     YDASFEVKKGEIFVIMGLSGSGKSTLVRMLNRLIEPSAGSILLEGKDISTMSADQLRE
                     VRRHDINMVFQSFALFPHKTILENTEFGLELRGVPKEERQRLAEKALDNSGLLDFKDQ
                     YPNQLSGGMQQRVGLARALANSPKILLMDEAFSALDPLIRREMQDELLDLQDSMKQTI
                     IFISHDLNEALRIGDRIALMKDGQIMQIGTGEEILTNPANDFVREFVEDVDRSKVLTA
                     QNIMIKPLTTTVELDGPQVALNRMHNEEVSMLMATNRRRQLVGSLTADAAIEARKKGL
                     PLSEVIDRDVRTVSKDTIITDILPLIYDSSAPIAVTDDNNRLLGVIIRGRVIEALANI
                     SDEDLN"
     gene            complement(10812..11903)
                     /gene="ulaG"
                     /locus_tag="VC122_000857"
     CDS             complement(10812..11903)
                     /gene="ulaG"
                     /locus_tag="VC122_000857"
                     /EC_number="3.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001863918.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-ascorbate 6-phosphate lactonase"
                     /protein_id="HEP5640794.1"
                     /translation="MTKVQDITRESWILNTFPEWGTWLNEEIEQEVVPADNFAMWWLG
                     NCGIWIKTPGGANVVMDLWSNRGKATKQVKDMVRGHQMANMAGARKLQPNLRAQPMVI
                     DPFMINELDYYLVSHYHSDHIDINTAAAIINNPKLNHVKFVGPYECGEVWKNWGVPKD
                     RIMILKPGDSFEFKDIKITAVESFDRTCLVTLPIQGADAQDGDLAGLAITDDDMARKA
                     VNYIFETPGGTIYHGADSHFSNYFAKHGRDYDIDVVLNNYGENPIGIQDKMTSVDLLR
                     MAENLRAKVVIPVHYDIWSNFMASTDEILELWKMRKERLQYDFHPFIWEVGGKYTYPQ
                     DQNRIEYHHPRGFDDCFLEDSNIQFKALL"
     gene            complement(12156..13802)
                     /locus_tag="VC122_000858"
     CDS             complement(12156..13802)
                     /locus_tag="VC122_000858"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014612609.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription antiterminator"
                     /protein_id="HEP5640795.1"
                     /translation="MMILDKKSYDLLSYLLKLETPETVMAISHALNQSRRKVYYQLDK
                     INKALPKGVDQIISYPRLGILLTADQKAACRLLLEEVTDYNYVMKSDERRRLSSIYIA
                     VSTERVTIDKLMQINDVSRNTILNDLTELREELEDKQYKIQLHATKARGYYFGCHPMA
                     LIQYLYKLLVDVYQGGNTSFIDIFNRKLSEIQGLSVYFSKDILTYFHEYLFLSQASLG
                     KTINTQDSQFMLQILPFMLLSYRNMRLDSETKSALKQEFHLIWKRKEYHIAQDLAREL
                     YHNFKLHLDDIEVSMVAMLMLSFRKDQDHHVESQDYDDMRATISHFIDQLESRYQLHF
                     THKQDLLKQLTTHCKALVYRKAYGIFLVNPLTDHIKEKYEELFAMTQSCATILEQDWT
                     ISLTDDDIAYLTIHLGGELRHNNTEQEKTKLVIVSDDGIGIQKLLFKQCQRYLANGQI
                     EAVFTTEQYQSVYDLLAVDMIVATTDTLKTKIPMLIVNPILSDDDIIKLIRFSKQGRL
                     SEHSRFSTELTKAIEAVVKDESDRYALVSKIEKLIHRELL"
     gene            complement(14127..14831)
                     /locus_tag="VC122_000859"
     CDS             complement(14127..14831)
                     /locus_tag="VC122_000859"
                     /EC_number="5.1.3.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003045586.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-ribulose-5-phosphate 4-epimerase"
                     /protein_id="HEP5640796.1"
                     /translation="MAKNLQEMRERVCAANKSLPQHGLVKFTWGNVSEVCRELGRIVI
                     KPSGVDYDLLTPENMVVTDLDGNVVEGDLNPSSDLPTHVELYKAWPEVGGIVHTHSTE
                     AVGWAQAGRDIPFYGTTHADYFYGPVPCARSLTKAEVDGAYEQETGSVILEEFSKRGL
                     DPMAVPGIVVRNHGPFTWGKTPEQAVYHSVVLEEVAKMNRLTEQINPRVEPAPRYIMD
                     KHYLRKHGPNAYYGQK"
     gene            complement(14833..15696)
                     /locus_tag="VC122_000860"
     CDS             complement(14833..15696)
                     /locus_tag="VC122_000860"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002992543.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-ribulose-5-phosphate 3-epimerase"
                     /protein_id="HEP5640797.1"
                     /translation="MARPIGIYEKATPKQFTWRERLQFAKDLGFDFVEMSVDESDARL
                     ARLEWTKEERLDLVKAIYETGIRIPTICFSGHRRYPLGSNDPAIEAKSLKLMKQCIEL
                     AQDLGVRTIQLAGYDVYYEEKSPETRARFIKNLRQSCDWAEEAQVMLSIEIMDDPFIN
                     SIEKYLAVEKEIDSPYLFVYPDAGNVSAWHNDLWSEFYNGHKSIAALHLKDTYAVTET
                     SKGQFRDVPFGQGCVDWQELFAVLKKTNYNGPFLIEMWSENCDTVEETKAAIKEAQDF
                     LYPLIEKAGLT"
     gene            complement(15701..16363)
                     /locus_tag="VC122_000861"
     CDS             complement(15701..16363)
                     /locus_tag="VC122_000861"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000166733.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-keto-L-gulonate-6-phosphate decarboxylase
                     UlaD"
                     /protein_id="HEP5640798.1"
                     /translation="MTHLPNLQVALDHSDLQGAVKAAVAVGHEVDVIEAGTVCLLQVG
                     SELVEVLRSLFPEKIIVADTKCADAGGTVAKNNAKRGADWMTCICCATIPTMEAALKA
                     IKEERGGRGEIQIELYGDWTYEQAQLWLDAGISQAIYHQSRDALLAGETWGEKDLNKV
                     KTLIDMGFRVSVTGGLDVDTLKLFEGVDVFTFIAGRGITEAEDPAAAARAFKDEIKRI
                     WG"
     gene            complement(16472..16957)
                     /locus_tag="VC122_000862"
     CDS             complement(16472..16957)
                     /locus_tag="VC122_000862"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011285369.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HEP5640799.1"
                     /translation="MNLKQAFIDNNSIRLGLSADTWQEAVQLAVQPLIDSKAVTSAYY
                     DAIIASTEKYGPYYVLMPGMAMPHAEAGLGVNHNAFALITLTKPVTFSDGKEVSVLLT
                     LAATDPSIHTTVAIPQIVALFELDNAIERLVACQSPKEVLEMVEESKNSPYLEGLDLD
                     S"
     gene            complement(17080..17358)
                     /locus_tag="VC122_000863"
     CDS             complement(17080..17358)
                     /locus_tag="VC122_000863"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000241478.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HEP5640800.1"
                     /translation="MVKVLTACGNGMGSSMVIKMKVENALRQLGVTDIQSASCSVGEA
                     KGLASGYDIVVASNHLIHELDGRTKGHLVGLDNLMDDNEIKTKLQEVL"
     gene            complement(17429..18862)
                     /locus_tag="VC122_000864"
     CDS             complement(17429..18862)
                     /locus_tag="VC122_000864"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019418868.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIC"
                     /protein_id="HEP5640801.1"
                     /translation="MEMLLAPLNWFSQNILQNPAFFVGLLVLIGYLLLKKPIYEVFAG
                     FVKATVGYLILNVGAGGLVTTFRPILVALAKKFELKAAVIDPYFGLAAANTKLEEMGF
                     ISVATTALLIGFGVNILLVALRKVTKVRTLFITGHIMVQQAATISVFVLLLIPQFQNA
                     FGAWAVGIICGLYWAVSSNMTVEATQRLTGGGGFAIGHQQQFAIWFVDKVAPFFGKKE
                     ENLDNLKLPTFLNIFHDTVVASATLMLVFFGAILAVLGPDIMSDVDLIGPGAFNPAKQ
                     AFFMYILQTSLTFSVYLFILMQGVRMFVSELTNAFQGISSKLLPGSFPAVDVAASYGF
                     GSSNAVLSGFAFGLIGQLITIALLVIFKNPILIITGFVPVFFDNAAIAVYADKRGGWK
                     AAVALSFISGIIQVALGAVAVGLLGLTGGYHGNIDLVLPWLPFGYLFKFLGIAGYVLV
                     CIFLLAIPQLQFAKAKDKEAYYRGEAQ"
     gene            complement(19169..21670)
                     /locus_tag="VC122_000865"
     CDS             complement(19169..21670)
                     /locus_tag="VC122_000865"
                     /EC_number="6.1.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014639013.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="leucine--tRNA ligase"
                     /protein_id="HEP5640802.1"
                     /translation="MTFYDHTAIEPKWQAFWADNHTFKTGTDASKPKFYALDMFPYPS
                     GAGLHVGHPEGYTATDILSRFKRAQGHNVLHPMGWDAFGLPAEQYAMDTGNDPAEFTA
                     ENIANFKRQINALGFSYDWDREVNTTDPNYYKWTQWIFTKLYEKGLAYEAEVPVNWVE
                     ELGTAIANEEVLPDGTSERGGYPVVRKPMRQWMLKITAYAERLLEDLEEVDWPESIKD
                     MQRNWIGKSTGANVTFKVKDTDKDFTVFTTRPDTLFGATYAVLAPEHALVDAITTADQ
                     AEAVADYKRQASLKSDLARTDLAKEKTGVWTGSYAINPVNGNEMPVWIADYVLASYGT
                     GAIMAVPAHDERDWEFAKQFKLDIIPVLEGGNVEEAAFTEDGLHINSDFLDGLDKASA
                     IAKMVEWLEAEGVGNEKVTYRLRDWLFSRQRYWGEPIPIIHWEDGTSTAVPESELPLV
                     LPVTKDIRPSGTGESPLANVTDWLEVTREDGVKGRRETNTMPQWAGSSWYYLRYIDPH
                     NTEKLADEELLKQWLPVDIYVGGAEHAVLHLLYARFWHKVLYDLGVVPTKEPFQKLFN
                     QGMILGTSYRDSRGALVATDKVEKRDGSFFHVETGEELEQAPAKMSKSLKNVVNPDDV
                     VEQYGADTLRVYEMFMGPLDASIAWSEEGLEGSRKFLDRVYRLITTKEITEENSGALD
                     KVYNETVKAVTEQVDQMKFNTAIAQLMVFVNAANKEDKLFSDYAKGFVQLIAPFAPHL
                     GEELWQVLTASGESISYVPWPSYDKSKLVENDVEIVVQIKGKVKAKLVVAKDLSREEL
                     QEVALANEKVQAEIAGKDIIKVIAVPNKLVNIVIK"
     gene            complement(21882..23021)
                     /locus_tag="VC122_000866"
     CDS             complement(21882..23021)
                     /locus_tag="VC122_000866"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011017287.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PLP-dependent transferase"
                     /protein_id="HEP5640803.1"
                     /translation="MKENTTLLHGYTVIDEFTGAASVPIYQTSTFHNSELYCPSQKHL
                     YTRFSNPTTEALEDGLACLEKATYAVAYASGMAAISTVLMLLKAGDHVIFPLEVYGGT
                     CQFATAILPNYQIETSFVDMADLATVKASIRPNTRMIYLETPSNPLLKICDISELVQL
                     AKAYGVLTVADNTFMTSLYQEPLAMGVDIVVESVTKFINGHSDVVAGLAATNNEAIYN
                     QLKLFQKNFGAIVGVEDAWLILRGMKTMGIRMEQAVKNAQQLANYLAKHPKVLKVHYP
                     GLDSHPNHDTHLQQAKNGGAVLSFELASKEELMTFTHRIQLPILAVSLGGVESILSHP
                     ATMSHACLSSQARLEQGVVDGLLRLSCGVENIEDLLADFEQALAF"
     gene            complement(23615..23791)
                     /locus_tag="VC122_000867"
     CDS             complement(23615..23791)
                     /locus_tag="VC122_000867"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEP5640804.1"
                     /translation="MNKLVAEIHKLVIRLHNQANGEVLVFEDEFEVILSSSSVKLCQP
                     AHTAPLLVHVIELV"
     gene            23970..24137
                     /locus_tag="VC122_000868"
     CDS             23970..24137
                     /locus_tag="VC122_000868"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011284460.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEP5640805.1"
                     /translation="MKKKLSLFMIATAAVAGLSLATLGSHQTVSANDASYSNGFYGHN
                     GPDSWLLYTVW"
BASE COUNT         7342 a         5332 c         4529 g         7272 t
ORIGIN      
        1 ctctatgcta aaggaagtcg atatcaatat aaatactata accgtcgacg ttcttcatga
       61 actcgtccca atcttgttaa tcaatgtttc caagcgactg ataagaataa attatggcta
      121 ggagacttaa cctacattcc tacacaagaa ggaatacttt atgtgtctgt cttgatagac
      181 gtttatagta gaaagattgt tggttgggca atgggccgac gtatgcaaga caagttggta
      241 acagaagctt tcaatcaagc ttataataga gaaaagccta aagaaggggt aatcgtccat
      301 acagatcagg gttctcaata taccggagca tgatttcagg acctacttag acagaaaaaa
      361 tgtaagtcca ggatgagtcg aaaaggtaat ccatacgata atgcactcat ggaatccttt
      421 tataaaacat taaaaagaga gcttgtcaat gatgctcatt tcgcaactat tgagcaagct
      481 cagcttgaga tttttaaata tagtgagact tactataacc ctaaacgtct gcattcagct
      541 ttagggtatc tctcacctgt agaatttgaa aaaatagtta ctcattagct taactttatg
      601 tctatttttt cttgacaagt ccagggtatg aagtcccctt atacacatac tttcaaaaca
      661 gctctcaatc ctctgtccgc tacaaaaata cgtcaccgct gccctccagc tgcctcattc
      721 aacgacaaac ttgaagccac aaacagactc atcaagggca ttaagtacaa tgcctttaga
      781 tttcagaact ttgacaactt taaaaccaaa atcctcatcg ctttgaacat ccaaaaagag
      841 agaaccaatc tgattctctc tcgttgttaa tgataagtca cccactacag ttgacaatga
      901 gtctcttact tcctgtttac tcatttaata gctcttctgg ttcccaagat tgattaaact
      961 catggtcctt aaaaagagaa gccaatcctg ttatttgttt cagtcttaaa atttcatctt
     1021 tatccattcc taaatgtttt gcaatccagt tatcacttcg cccacactca tgaagatcct
     1081 ttacaatctg actcatcaaa tcaacatcat gacttcctct agctctatta tgtcgaatgg
     1141 tagaagccat tcgatagtct aatgacttat caataacaga aacaggtaat ctcccacttt
     1201 ccctttcata aatatctgaa taatctagca ttatacgata tctatgaaaa ccatcaacaa
     1261 tactataccg atcctcttcc ttatcgtaat agcaaacaat cggcatagta taaccatcag
     1321 attttatact atcatacaat aatttcattt caggcggagc aactgaattc ggattataat
     1381 catttggaga aatcttttca attggaattg atttaatctc atagactgga ctaataaaat
     1441 tactcacttt ctaactcctt taaactcgtc ttatatttct cttttaaaaa ggctactatt
     1501 ttttgttgct ctcttgtcaa accaaacccc atagttcgac aaatatgatc gttttttaaa
     1561 atacagaaac acattcgttt ccagcttggt atatcctttg ttgttacaat atcgtctgta
     1621 tcatcaggaa tttgatctaa aaaaataacc cttgaatgtt taaaagaggt ataatttgaa
     1681 ataccatttc tagcaatacg atatcctttt tcaattagtt cattaattgt ttcctccgca
     1741 agtcctccgc ctgttttatg ccaaaagtct attgatttat taaattttct aacataatta
     1801 tttcttaacc tcactggtag cgttgataaa agaaactgag tgtacgactt ccacgaatga
     1861 ccctttggta aagcaattga tttatatccc attgctttag tacgtccata aatattcgaa
     1921 aagttaactc cttgaacacg tcccaaaagc tttacccaag tttcttgatc tattattcta
     1981 taaagattta ggctatcaac agcatagtct tgaaaaggag atgcaactct catttgtgat
     2041 ggttttaaac ccgctttata gaatagatca taaagttcgt tataagaata agaaaactta
     2101 tagtaagcat gccatatgtc ttccactgac caatcatata agggagatgc tgtccagaca
     2161 tctttgaatt gtttggtaat ccagcattcc ttttgataac catacttttt attgataaaa
     2221 ccactatatc tgtgtaagga ttctgaagca cggtttccta atatacaaac tgttttttga
     2281 tttccgtgaa tttgtttata ccacctacca aattgtttcg ctaaatcctc ctgattcatt
     2341 ttataacgat aagtagtaat tgaattattt tccaaattaa taacataatc ttgagatggc
     2401 attggtctta cccagatatc ttctgttttg tcatcccacg gataccaaaa catttcataa
     2461 ctactaagtg cagttctagt agccatcggt aagcacaccc aatacaaaga aacttttttt
     2521 ctaccttcta aagaagtgaa tgtttcttga acatattttg ttgttaaact gtactgtgct
     2581 tcaaaatctt gatgaaacac acctatttct ctatccggat aatatttatc tctaaaatcc
     2641 agtaaaatat taagaagtaa cccactatct tttccacctg agaaggaaat atagacaata
     2701 ggaaattcat taaacaaaaa atgtaatctt tctatcaatg catcataaac attttgattt
     2761 aaatactttt tcactaaact tcctcattat gcatttaaaa atattaatac atagattcac
     2821 taagaatctc tttttcgagc tttttgtttt ttaatttttc aagttgaata attaaacctt
     2881 gcttttcttt ttcaaggtaa tagactctac tccttgtaag tctaacttat tcttaatctt
     2941 atttatcgct cattttttaa attccgatgc tctcacagat attatcattg tttagttaca
     3001 tgtctgtcaa tctattcgcc accacactag gggccatctg attgaaaaat aaaatttcgg
     3061 agtccattgt ttgctcaaga atcatatctg tcacaattac gtcatactct gaagacagtg
     3121 acaccatatc aaccatggtc agcctcaagt catcaaagtt gataatctca aaaaatcctc
     3181 caaaatactc ttttaagaag ttgccaacat tgttataact tctctcaacc actaatagtt
     3241 tcaagcgacg acgcgattga tttgtaagaa agagattttc ccaggtgagg aacaaacaaa
     3301 tagaggagat gattgatggt tctttccttt aattccttat gatccccagc aaataactct
     3361 ttaacataag caacaaaggt ctggtattga actgggggtt tttgatttgt aaaggcacta
     3421 tatttataat gcctttattt aagggttaac tctaaaaatc agattggtct tttgcttggc
     3481 aatccggaca aatcccgtaa gcaataactg ggatacgcgt gaccttataa cctgtttgct
     3541 catgggcttc tttggcaata tccataacat caacatccat aaagtcagca atcttcccac
     3601 atatttcaca aaccacattg acgtgttgat ggcccataaa gtcatagtag gtggttaaat
     3661 cattgctgat tttcaactct gagacaaaac cttcatcaac taaaactttc aaattattat
     3721 agactgtggc aaggctcatg ttaggaaagt taggctgtaa gtcacggtaa atcttatcgg
     3781 cactagggtg ttctgtggac tgaatcatgt aagaaataat ggctttacgt gtctctgtaa
     3841 ttcgaatatg tttttcccgt agatgctcta ggacattttc ataggcatct agggcttgct
     3901 gatgtgaatg aatgtccata agctgctact cctatccttt tttcctttat tatcacgcac
     3961 gtctctagtt tcaaagattt gtgatgcatt ctggtttagc gactaattgc aaatagtcta
     4021 tttttaggca gcgattcatc acaatagcct ctttgcctgc cgaccgtaaa atggtttgta
     4081 cttcttggtt ttcaaggcct aattgcgccc aaaagacttt ggcctgacct gctaaaaagt
     4141 ctctggcaac ttctggtaaa aactcacttc tgcgaaagac atctacaatg tcaacctcaa
     4201 agggaattgc cttgatactg gcatagacct tctctcccaa aataagctga cctgctaatt
     4261 tgggattgac tggaatgatc cgataatcca tagcctgcat aaatttggcg acaccataag
     4321 ctgctgtgtc tttgcggtca gaaagaccaa caaccgcaat ggttttggct gactccaagt
     4381 aagcttttag gatatcctct gagggatttt gaaacgaata aatcacttgt tcccctatct
     4441 atttaataac ctctacagct gttcaatata gcaggataac tcctaaagca tggcttcaat
     4501 cacttttaac cttatccgtc aaaaagctca aagcagcttc atgacttctc ctacaatttt
     4561 ttagtttatt ataacataag ttgtcaaaat gagaaataac aagtaagaaa acttatttgg
     4621 cttcgtacca gctctgccca gtactttcat cgacacataa aggaacagct aaatcaacag
     4681 cagcttccat ggtgtctttg acaagttttt ttatagcagt caactcatca tttggcactt
     4741 ccaaaacgat ttcatcatgc acttgaagca gcattttagc tctaaaacca cctgcttgca
     4801 aggccttatc cagattaatc atagcgattt tgagaatatc agctgcgctt ccttgaatgg
     4861 gcgagttgat ggctgtacgc tccgcaaaag agcgaacgtt gaaattacga gagttgatat
     4921 ccggtaattc acgacgacgc ttaaagaggg tctctacata gcccttgtct ttagcctcac
     4981 gcaccacatt ttccatatag gctttaatcc caggatagcg ttcaaaatag gtatcaatgt
     5041 aagacttggc ctgtttacgg gtgataccaa ggttatttga caaaccaaaa tcagaaatcc
     5101 catacacaat tccaaagtta acagccttgg cattacggcg gtcattggca gtaacatctg
     5161 ctgccttgtc aatcccaaac acacgcatag ccgttgaggt atgaatgtct gccccttcat
     5221 taaaggcagc aatcaaatgc tcatcccctg aaatatgtgc cagtacccgc aattcaatct
     5281 gtgagtaatc tgaactcaac aagacggcat cttcatgaga tggggtaaag gccttacgaa
     5341 tcaaacgccc ctgttccaaa cgaatgggaa tgttttgaag attagggtcc acactagaca
     5401 aacgccctgt ctgggtgaga tcctgcacat agcgggtatg aatcttgcca tcagccagaa
     5461 tataatcctg taaaccaatc acataggtgg attgcaattt agtaatctgg cggtaatcca
     5521 aaattttagc cacaattgga gcaatcggtg ccaaacgttc caaaacatca acagctgtag
     5581 aataacctgt tttggtcttc ttagtcatct caagaggtag ttgcatcttc tcaaacaaga
     5641 tactgcccag ctgtttaggc gaattaatgt taaattcttg gccagccata tcataaatct
     5701 cttgggtgag cgcttcaata atgaccttgt tttgctctgc catatcttgc aaagtcgccc
     5761 tattaacctt gatcccttca atttccattt tagcaaggac attagccaaa ggcaattcaa
     5821 tattctgata gaggtctagt tgctcatggg cagtaagctt gtctagcatt tgagacttgc
     5881 tatctaataa tactttgacc ttacgagcta agtgttctag taaaacgtct ttatcaggaa
     5941 cagcccgttt agcaccttta ccatagactg tatcgtcttc ttccaaagag atgtcagtaa
     6001 aaatgcgggc aatggtgcta agctcattgt cttctacagt tgacagaagg taattagcta
     6061 atctagcatc atagctaggt gctaccaact caattcctaa gtggctcaac aagaccttgc
     6121 tacgtttaaa atcataagtg gcaatgggtt tttggaagac ctgcttaaaa ctatccgtcg
     6181 ctaataggct aaggtcagtg gatgcgtaaa tctgctcacc atgtccccaa gcaaaaccaa
     6241 tgatggcctc acaatggtaa ttatcccgaa gcgtttcaaa gtagaagatg tcttctgcgc
     6301 taaacaaatc agcagacaca ttggtcactt cttgatagga aatggcagct actggctctt
     6361 gaggcatctg gctagcaaga cctttcttga gttgcacaaa atccatctca tcgtaaaatt
     6421 gagagaggct agcaacatct ggaccattgt agacaatatc atctaagcca attgtgatag
     6481 gagatgctgt attgatagtg gcgagcgttt tagataggaa agcttggtca cggtcattga
     6541 tcaagttttc tttcattttt gaggccttga atccatcaat atgctcgtaa atgccttcca
     6601 aactaccaaa ttcatgaagg agcttgagcc cagttttttc accgattttg gtcactcctg
     6661 gaatattatc cgatttatct cccatcaagg ctttgaggtc aataaactgg tttggcgtca
     6721 agcccatttt ttccatgagg taagccggtg tgaattcttc aaattcggca accccttttt
     6781 tagaaatttc aaccacggta ttttcgtcag ttaactggat caaatcttta tcaccactga
     6841 caatggtcac atcaaaaggc acttctgtgc gctccgccat tttatcaagc gtcccgataa
     6901 tatcgtctgc ttcgtagtgt tccaactcat aataagcaat cccaagggct gtcaacatct
     6961 cacgaatata aggaaactgc tcacgaaatt cctctggtgt cttggcgcga cccgccttat
     7021 aatcggcata catttctgtt cggaaggtgg ttttgcccgc atcaaaagcc accagcacgt
     7081 gagtaggctg gactcttttc atcatgtggt ctagcatcaa atgaaagcca taaatggcat
     7141 tggtatgaag accgctatga tttttaaagc ggtcaatttg attgtagagg gcaaaaaagg
     7201 cacgaaaggc aacagatgag ccatcaatta atagtaattt attcttgttt tccatagcct
     7261 tattataaca cagagggacg ggcttttctc ctttaagcca ttggttttgc tgataagtaa
     7321 atagttaacc taaaaagcct aggaaaattc ctagacttct ctatttgcta tttagacaaa
     7381 agcacttatt ttgtccaact tgatacttta gattggtttg cctctaccca ttttttggcg
     7441 gcagcttctg ggctcatgcc tttgttgatg tcaagcatga ctgcttccat atctttttgg
     7501 gtccagtgga acttatcaat gattttgtag acatttggaa gatctttttt caagccttta
     7561 cgagcaatgg tattgatgtt ctcagtcgag ccaaaggttt ctttaggatc tttaaggtac
     7621 ttcaagtcat acttggcaaa catccagtgt ggtgaccaag ctgtcacgac aataggatct
     7681 tttttcttga tcgcttgatc caaggaagtc gtcatggctc ctgttgatgc cgccactaac
     7741 tcccaactag atagattatg atactctttt agagtctttt gggctgctgc catgatacct
     7801 gcacctggtt cgatccctgt gattttttga tcggcttgtt tgcttaagtc ctcaatacta
     7861 ttaacatctg tcatgtattt tggaacagct aaaccaagtt tggtcccttt taggttaggt
     7921 cctaaatcat ctaattttga cttgtatttc tgatattgtt ggccatgggt gactggcaac
     7981 caagcactag ttgaaaaatc agcgttccca tttgcaacgg tttgccacat aacggcattg
     8041 tcaagcggag tcaaggtcac atggtaacct tcgtttttca aaacttctgc aatcacatga
     8101 gttgaggcaa cctcagaatc ccactgcacg taagcgatgt taacagtttc acccttatct
     8161 gccataccag aagtcatagc cataataccg cgtcctaaag cagctatcaa aaagacagca
     8221 agggctgcta gaccaatcca tttattggtt ttgccagctt tggctttttc ttgaggtttg
     8281 ctattgaaca actgtgtcat acggtcaagc acaatcgcca agataaccaa ggcaagacca
     8341 ctgacaaatc cactaccaac atcagcgtgt tgaagggctg acaagacttc acgtcctaag
     8401 ccaggagcac caatcatgga gcctgttact accatggaaa gggctaacat catggtctga
     8461 ttaacacctg ccataatagt atttttagct aaaggcaatt ccactttaaa gagcttttgt
     8521 ttcccagttg acccaaaggc atctgaggcc tcaatcaatt ctgttggaat gtcacgaatg
     8581 gccaaatttg taaaacgaac cgttggaggc aaggcaaaga tgactgaggc aaacactcca
     8641 ggcaccatac caataccaaa aaaggcaacg gcaggaatca agtaaacaaa agccggcatg
     8701 gtttgcataa agtccaaaat aggattcaca atctgacgga cagtggcatt tttagccatc
     8761 caaatcccca gcggaatacc aataagtacc gaaatcaaac tagctactaa caccagagta
     8821 aaggtattca ttagctgctt ccataaccct tgattgtaaa taaagagcaa tcctaacaag
     8881 gtaaaggttg gcaaaggcca ttttttctta gcaaggaaaa acacccctgc tgttactaga
     8941 acaataaaaa gtaacgggtg gataaataaa agtgttttgg tcatccaatc catcaaaaag
     9001 ctaccaacta cttgcataat atcaaataga cctgagaaag ttttagttag ccactccgtt
     9061 agctgctcca ccagttgagc tactggtaat tttgtttgta atatcgtttc caaaatgcat
     9121 cactcctttt agttaaggtc ttcgtctgag atattagcca aggcttcaat cactcgtcct
     9181 cgaataatga cacctagcag acgattatta tcatctgtca ctgcaatcgg agcagatgaa
     9241 tcatagataa gaggcaaaat atctgtaata atagtatctt ttgagacagt tctcacatcg
     9301 cgatcaatca cttctgatag cggtaaccct tttttgcgcg cctctatagc ggcatcggcc
     9361 gtcaaactac cgactaattg gcggcggcga ttcgtcgcca tcaacataga cacctcttcg
     9421 ttgtgcatac ggttcaaggc aacttgaggt ccatctaatt caacagtagt tgttaacggt
     9481 ttgatcatga tattttgtgc tgtcaagact ttagaacggt ccacatcctc aacgaattca
     9541 cgcacaaagt cattggctgg gttagtcaag atttcctcac cagtaccaat ttgcataatt
     9601 tgtccgtctt tcatcaaagc aatccgatca ccaatacgca aagcttcatt caagtcatga
     9661 ctgataaaga tgatggtttg tttcatgctg tcttgcaaat caagtaattc atcttgcatc
     9721 tcacgacgaa tcaaaggatc aagcgctgag aatgcctcgt ccattaagag aattttaggg
     9781 ctattagcta gcgcacgggc taggccaaca cgctgctgca tcccaccaga tagttggttt
     9841 gggtactggt ctttaaaatc taataggcct gaattatcaa gggctttttc tgccaatcgc
     9901 tggcgttctt ctttgggaac gccacgtaat tccaaaccaa attcggtatt ttccaaaatg
     9961 gttttatgag gaaagagggc aaagctttgg aaaaccatgt taatgtcatg gcggcgcacc
    10021 tcacgcagct gatctgctga catggttgag atgtctttac cttccagcaa gatagatcct
    10081 gctgaaggtt caatcaaacg atttagcatg cggacaaggg ttgatttccc acttcctgat
    10141 agccccataa taacaaagat ttcacctttt ttgacctcaa aactagcgtc atagacacct
    10201 acagtagcgc ctgttttctt aaaaatctca ctcttatttt tgccagtctt taccatctca
    10261 agagctgctt tttgtttttt gccaaaaatt ttactgagat gtttgacttc taaaattgtt
    10321 tccattcgtt ttttctccta ggtaatcgtt tatcatgtga aatagtgaca tagctttggc
    10381 gaaatattag ccaatattta acaaagtagc tctacacaat taataaacgt gtggtcactt
    10441 ttttacttaa aaaaagaatc tgccgaaaca gaacataact atattatcac gtgtggtcac
    10501 tttttgtcaa ggacaataac tcgatctgta ataccacaat gataggtgat cgctttcatt
    10561 cttttaggta tggtctttta accagtacaa ttttttatca caactgatga ctatttcttt
    10621 gctgattagg ctattgttac attagaaatc cttcttttac ctataatatc tcttttgcgg
    10681 ccatcgtaaa taacgaccac ggtgactctt aaacatttgc tcatctctcc aaaaaaactc
    10741 acctaagaca gtgtcctatg aagcgagtta gcttaatcag gcactatcat cttaggtgag
    10801 tagggtgttg cttatagtaa agctttaaat tggatattag aatcttctaa gaagcagtca
    10861 tcaaatccac gaggatgatg gtattcgata cgattttggt cttgtggata ggtgtacttg
    10921 ccaccaacct cccagataaa gggatggaaa tcgtattgga gacgttcttt gcgcattttc
    10981 cacagttcaa ggatttcatc tgttgatgcc ataaagtttg accaaatgtc ataatgaact
    11041 gggataacga ctttagcacg gagattttct gccatacgaa gaaggtcaac agaagtcatc
    11101 ttgtcttgaa tcccaattgg gttttcccca tagttattta acacaacgtc aatatcataa
    11161 tcacgtccat gtttagcaaa gtagttcgaa aaatgagaat ccgcaccatg atagatggta
    11221 ccacctggtg tttcaaaaat atagttaaca gctttgcgag ccatgtcatc atctgtgata
    11281 gctaaacctg ctaaatcacc atcttgagca tcagctcctt gtattgggag agttaccaga
    11341 caggtgcggt caaacgactc tacagcggtg atcttaatat ctttgaattc aaagctatca
    11401 cctggcttta agatcataat acgatctttt ggcacacccc aatttttcca aacttcacca
    11461 cattcataag gtcctacaaa tttaacatga ttaagttttg ggttattgat aatggctgcc
    11521 gcagtattga tatcgatgtg gtcactatga taatgagaaa ccaaataata atccaactca
    11581 ttaatcataa atggatcaat caccattggt tgggccctaa gattgggctg taatttacga
    11641 gctcctgcca tattagccat ttgatgtcca cgaaccatat ccttaacttg cttggtcgct
    11701 ttcccacgat ttgaccataa gtccatgaca acattagcac cgcctggtgt cttaatccag
    11761 ataccacagt ttcctaacca ccacatcgca aagttgtcag ctggcacgac ttcttgctcg
    11821 atttcttcat ttaaccatgt tccccattct ggaaacgtgt taaggatcca tgattcacgt
    11881 gtaatatctt gtactttagt cattagggtt tcctccgaat ttgttcttcg tttctatact
    11941 ttaagtatag actttcttgt caatgataaa aagaccaagt gtcacacaga tctgtgttca
    12001 aaagtggact gactttttca ttagctcagt ctaatactgt ctcattttgc acactgtatc
    12061 tcgcatcaat actgtattca aagctatatt aaaaacctca gttatatttt tctatgtcat
    12121 agaaaactga ggtactttga cgacttaaca cttactcata gcagctcgcg atgaatgagt
    12181 ttttcaattt tagaaaccaa ggcataacga tctgattcat cttttacaac agcttcaata
    12241 gcttttgtga gctctgtaga aaagcgggag tgctcactca agcgtccttg ctttgaaaag
    12301 cgtatcaatt tgatgatatc atcgtcactt aaaatagggt taacaataag catcgggatt
    12361 ttagtcttta gcgtatctgt agtagcaaca atcatatcga ctgctaaaag gtcgtaaaca
    12421 ctctgatact gttctgtcgt aaaaacagct tcaatctgac catttgctag atacctttga
    12481 cactgtttga acaataactt ttgaatgcct atcccatcat ctgaaacaat aacgagtttg
    12541 gtcttttctt gctctgtatt attgtgacgc agctctcccc caagatggat agtcaagtag
    12601 gcaatatcat cgtctgtcaa gctaatcgtc caatcttgtt ccaaaatagt ggcacaagat
    12661 tgagtcatag caaacaattc ttcgtatttt tctttaatat ggtctgttaa gggattaact
    12721 aaaaaaatcc cataagcttt acggtaaacg agcgccttac agtgggtcgt caactgcttc
    12781 agcaaatcct gtttatgcgt gaaatgtaac tgataacggc tctccaactg gtcaataaag
    12841 tgactaatgg ttgctcgcat gtcgtcataa tcttgacttt ccacgtgatg atcttgatct
    12901 tttcgaaaag acaacatcaa catcgctacc atactaactt cgatatcatc caaatgaagc
    12961 ttaaaattat ggtaaagctc tctagctaaa tcctgagcaa tgtgatattc tttacgtttc
    13021 caaatcaaat gaaattcttg tttaagagca gactttgttt ctgaatcaag acgcatatta
    13081 cgataactta aaagcatgaa aggtaaaatc tgcaacatga attggctatc ttgggtatta
    13141 attgtctttc ctaagctagc ttgagataaa aagagatact catgaaaata agtcaaaata
    13201 tctttagaaa aataaaccga taagccttgt atctctgaga gtttacggtt gaatatatca
    13261 ataaagctag tattgccacc ttggtaaacg tccactaaca atttgtacag gtattgaata
    13321 agagccattg gatgacaacc aaaataatat cccctagcct ttgtcgcatg aagctgaatc
    13381 ttgtattgtt tgtcttccag ttcttccctt aactcagtca ggtcatttaa aatcgtgtta
    13441 cgagacacat cattgatctg catcagttta tcaatggtca cgcgctcagt ggaaaccgca
    13501 atataaatcg aggataggcg tctgcgctca tcacttttca tgacataatt ataatcagtc
    13561 acttcttcta ataataagcg acaagcagct ttttgatccg ccgttaaaag aatgccaagt
    13621 ctaggataag agataatctg atctactccc tttggcaaag ccttattaat tttgtcaagc
    13681 tggtaataaa ctttacgacg cgactgattt aaagcatgag aaatggccat aactgtctca
    13741 ggtgtttcta gtttaagtaa ataagacagc aagtcataac tctttttatc taaaatcatc
    13801 atgtttacaa acctcctctc tgaaaatcca tccttgctta ttttatcatg atttcagtga
    13861 ctgcgcagcc aagttttgaa aaactcacca taataagatt cgcttaatat gattcgagct
    13921 aatcacctca actgatccaa taccaacacc ttatatctgg tctctaagaa ctccacaaca
    13981 tacggttcat ccagtttgtg ctcattggcg tttaaacagt tcatcgaaaa ggacaagtca
    14041 aaaggacttg cccaaacaat gattaacaaa accttgaact ttttggcttc tagtgagaac
    14101 ctgtgctaat aagccatatc gtgacctcat ttttgaccgt aataagcatt tgggccatgt
    14161 ttacgcaaat aatgcttatc cataatgtat cttggcgctg gctctacgcg tggattgatt
    14221 tgttctgtca agcgattcat cttagccact tcttctaaga caaccgagtg gtaaacggct
    14281 tgctctgggg ttttccccca cgtaaatggg ccatgattac gcaccacgat tcctggaacc
    14341 gccatcgggt caagaccacg cttgctaaac tcttctaaaa tcacgctgcc agtttcttgc
    14401 tcgtaagccc catcaacttc tgcttttgtg agagaacgag cacaaggaac cgggccataa
    14461 aaatagtcag catgggtagt cccataaaac ggaatatcac gacctgcctg tgcccaaccg
    14521 acagcttctg tggagtgagt gtggacaata ccgcccactt ctggccaagc tttataaagt
    14581 tccacatggg ttggcagatc tgaagaaggg ttcaaatcac cttcgaccac attcccatca
    14641 aggtctgtca ccaccatatt ttctggtgtc aagaggtcgt agtccacccc agaaggcttg
    14701 atgacaatac gtcctaattc acgacaaact tcagagacat tgccccacgt aaatttgaca
    14761 agaccgtgtt gtggtagaga cttgttagcc gcacagacac gttcacgcat ttcttgcaag
    14821 tttttcgcca tattatgtca accccgcttt ctcaataagt gggtaaagga agtcctgagc
    14881 ttctttgata gcagcttttg tttcctccac cgtgtcacaa ttttctgacc acatttcaat
    14941 caaaaaaggc ccattgtagt tggttttctt gaggaccgca aacagctctt gccagtccac
    15001 acaaccttga ccaaatggca catcacgaaa ctgccctttt gaggtttccg tgacagcata
    15061 agtatctttc aagtggaggg ctgcaatgga tttgtgccca ttgtaaaatt cactccacaa
    15121 atcattatgc caagcagaaa cattgcctgc atcaggataa acaaagagat aaggcgagtc
    15181 aatctctttt tcaactgcca agtatttctc aatagagttg ataaaagggt catccataat
    15241 ctcaatagaa agcatcactt gggcttcttc ggcccagtca caagattgtc tcaagttttt
    15301 gataaaacga gcacgggttt caggcgattt ttcttcatag taaacgtcat aaccagctaa
    15361 ttgaatggta cgaacgccta agtcctgtgc caattcaata cattgtttca tcagtttaag
    15421 ggacttggct tcaatagcag gatcatttga gcctagtggg taacgtctat gaccagaaaa
    15481 acaaatggtc gggatgcgaa tgcctgtttc ataaatggct ttcactaaat caagccgttc
    15541 ttccttagtc cactctaagc gcgctaagcg agcatctgat tcatcaacag acatctccac
    15601 aaaatcaaaa cctaagtctt ttgcaaattg caagcgttca cgccaggtaa actgttttgg
    15661 cgttgctttt tcataaatac caataggacg cgccataaag ttacccccag atacgtttga
    15721 tttcatcttt gaaggcacgc gccgctgcag caggatcttc tgcttcagta atgcctcgcc
    15781 ctgcgataaa ggtaaagaca tcaacccctt caaaaagttt caaagtgtct acatcaagcc
    15841 caccagttac tgagacacga aagcccatgt caatcaacgt tttcaccttg ttaaggtctt
    15901 tttcacccca agtttcgcca gcaagaaggg catcacgaga ttggtggtaa atcgcttggg
    15961 aaatcccagc gtcaagccat aattgagctt gctcataagt ccaatcacca taaagttcga
    16021 tttgaatttc cccacggcca ccgcgctctt cttttatggc tttgagggct gcttccatgg
    16081 ttggaatcgt cgcacagcaa atgcaggtca tccagtcagc accacgtttt gcattgtttt
    16141 tagcgaccgt accgccagcg tcagcacatt tggtatctgc tacgataatt ttctctggga
    16201 agaggctgcg taatacctca actagttcgc ttcccacttg aagcaaacaa acggtaccag
    16261 cttcaatcac atctacttcg tgacccacag caacggctgc tttaacagcc ccctgtaagt
    16321 cagaatggtc taaagcaact tgtaaatttg gaagatgtgt catgtgtttt tcctagaact
    16381 ctcagcggct atgaaaaagc tgatgaggag ttcttatcct ttcaataatg attgtgagtc
    16441 gttttatccg tttaagtcag taagctatcg attaagagtc taaatccaaa ccttctaaat
    16501 acgggctatt ttttgattct tctaccattt ctagaacctc tttaggactt tggcaggcca
    16561 ccaagcgttc aatggcgtta tctagttcaa acaaggccac aatttgtggg atagccactg
    16621 ttgtgtgaat agaagggtct gtagcggcca acgtcaaaag aacagaaacc tctttaccat
    16681 ctgagaaggt aactggtttt gttagtgtaa tcaaagcaaa tgcattgtga ttaacgccta
    16741 aacctgcttc tgcatggggc atagccatac ctggcatcaa cacataataa gggccgtatt
    16801 tttctgttga tgcaatgatg gcgtcgtagt aagctgacgt cactgctttg ctatcaatca
    16861 aaggttgaac agctaattga acagcctctt gccaagtatc agcacttagc cccaatcgga
    16921 tggagttgtt gtcaataaag gcttgtttta aattcataaa aactatctct ttctagtgtc
    16981 ttgctttgca acacacacca tttttactag ttttatccta ctaaagagaa agagctgaaa
    17041 accttcgtca caactccttc gttttgattc agaattagct tatagcactt cttgtagttt
    17101 ggttttaatt tcattatcat ccatcaggtt atcaagacca accaagtggc ctttggtacg
    17161 accatcaagc tcatgaatca agtggtttga tgccactaca atatcatagc cagaagctag
    17221 acctttagct tcaccaactg agcaagaagc tgactgaata tctgtcacac caagttgacg
    17281 aagcgcattt tcaactttca ttttgatcac catagatgac cccataccat tgccacatgc
    17341 tgttaagact ttaaccattg ttttttcctc cgaaaatgtt ttattattgt tttgttaaaa
    17401 tagcagagct atttcttaag atgcagtgtt attgagcttc cccacggtag taagcttctt
    17461 tgtccttagc ttttgcgaat tgcaattgtg gaatggcaag taagaagatg caaacaagca
    17521 cataccctgc aataccaagg aatttgaaga ggtacccaaa tggtaaccat ggaagaacca
    17581 agtcgatatt accgtgataa ccacctgtaa gtcctaaaag acctacagca acagcaccaa
    17641 gtgccacctg aataatacct gagataaagg aaagagctac cgctgctttc cagccaccac
    17701 gtttatctgc ataaaccgca attgccgcat tatcaaagaa tacgggaacg aatcctgtga
    17761 taattaagat tggattttta aagataacaa gaagagcaat ggtgattaat tgaccaatca
    17821 aaccaaaggc aaatcctgaa agcaccgcat ttgaagaacc aaagccatag gaagctgcaa
    17881 cgtcaaccgc tgggaatgac cctggaagca atttgcttga gatcccttga aaggcattgg
    17941 tcaattcaga aacaaacatg cgaacaccct gcatcaagat aaagagataa actgagaagg
    18001 tgagagaggt ttgcaaaata tacatgaaga aagcttgctt agctgggttg aatgctccag
    18061 gtccgattaa gtccacatca gacataatgt ctggaccaag gacagccaag atagctccga
    18121 aaaagaccaa catcaaagtt gctgacgcaa caacagtatc atggaaaatg tttaagaagg
    18181 ttggtaattt taggttatca aggttttctt cttttttacc aaagaacggt gcaaccttat
    18241 ccacaaacca aatcgcaaat tgttgttggt gaccaatcgc aaacccaccg ccacctgtaa
    18301 ggcgttgagt tgcttcaacc gtcatatttg agctgactgc ccagtaaaga ccacagatga
    18361 taccaactgc ccacgcacca aaggcatttt ggaattgagg gattaaaaga aggacaaaga
    18421 ccgaaatcgt cgctgcctgt tggaccatga tgtgacctgt gatgaaaagg gtacgaactt
    18481 tagtgacctt gcgaagtgca accaaaagaa tgttgacccc aaatccaatc aagagagctg
    18541 tcgtagcaac actgatgaag cccatctctt ctaacttagt attagcagca gccaaaccaa
    18601 aataaggatc aataacggcg gctttcaatt caaatttctt ggcaagagca accaagatcg
    18661 gacgaaaagt cgttacaaga ccgcctgctc caacgttcaa aatcaagtaa ccaacggtag
    18721 cctttacaaa accagcaaag acctcataaa tgggtttttt aaggagtaaa tacccgatta
    18781 aaaccaaaag acctacaaag aacgctgggt tttgcaaaat attttgcgag aaccagttta
    18841 ggggagctag gagcatttcc ataagaacta cctcttttca aagttaataa agataaagtg
    18901 tttatatgtt cattataggt aatgaaagcg ttttaaaaaa gaccaactat cacacaaaga
    18961 agtgttcaat attagactta agttaactta ttttaaaatt taactacact taataataaa
    19021 gagaaaatca ccctaaacaa cgattaaaaa ccacaagaac gaaggttctt gtggtttttc
    19081 tagatgagtt gctttttgaa tagatcagct ctttggcatt aactgtgacg tgacgcaaat
    19141 aaccactgaa gggctgtgtc acctcaaact atttgataac aatattaacg agtttgtttg
    19201 gtactgcgat gactttgatg atgtctttgc ctgcgatttc ggcttggact ttttcgttag
    19261 caagagcaac ttcttgcaat tcttcgcgtg aaaggtcttt ggcaacgaca agtttagctt
    19321 tgaccttacc tttgatttgg acaacgattt ccacatcgtt ttcaaccaat ttgcttttat
    19381 cataacttgg ccatggcacg taagagattg actcacctga ggctgtcaag acttgccaga
    19441 gttcctcacc taagtgcggt gcgaatggtg caatcaattg aacgaagcct ttggcatagt
    19501 cgctaaagag tttgtcttcc ttgttggctg cattgacaaa taccatgagt tgagcgatag
    19561 ctgtgttgaa tttcatttgg tcaacttgct cagtaacagc cttaacagtt tcgttgtaaa
    19621 ccttgtcaag ggctccgcta ttttcctcag tgatctcttt agttgtgatc aaacggtaaa
    19681 cacggtccaa gaacttacgt gagccttcaa gaccttcttc agaccaagcg atggaagcgt
    19741 caagtggtcc catgaacatc tcgtaaacac gaagagtgtc agcaccgtac tgctcaacaa
    19801 catcatctgg gttgacaacg ttcttaagtg atttagacat cttagctggt gcttgttcaa
    19861 gctcttcacc agtttccaca tggaagaagc tgccatcacg tttttcaacc ttgtcagtcg
    19921 ctacaagcgc tccacggctg tcgcggtagc ttgttcccaa aatcatccct tggttaaaga
    19981 gtttttggaa tggctctttg gttggtacga caccgagatc gtaaagaacc ttgtgccaga
    20041 agcgagcgta gagaaggtga agcacggcat gctctgcacc accgacatag atatcaactg
    20101 gcaaccattg tttgaggagt tcctcatcag ccagtttttc ggtgttgtgt ggatcgatgt
    20161 aacggagata gtaccagctt gaacctgccc attgtggcat ggtgttagtc tcacgacgac
    20221 ctttaacacc atcttcacga gtcacttcca accagtctgt cacgttagca agtgggctct
    20281 caccagtacc tgaaggacgg atatccttcg ttactggaag aaccagtggc aactcactct
    20341 caggaaccgc tgttgaggtc ccatcttccc agtggatgat tgggattggc tcaccccagt
    20401 aacgttgacg gctaaagagc cagtcacgaa gacggtaagt gactttctca ttaccgacgc
    20461 cttctgcttc aagccattcg accattttgg caatagctga cgctttgtcc agaccatcta
    20521 ggaagtctga gttgatgtga agaccgtctt ctgtaaaggc agcctcttca acattaccac
    20581 cttcaagaac tggaatgata tctagcttaa attgttttgc gaattcccag tcacgttcat
    20641 cgtgggctgg aacagccatg atagcaccag taccgtagct ggcaaggaca tagtccgcaa
    20701 tccaaactgg catttcgtta ccattaactg ggttaatcgc atagctaccc gtccagacac
    20761 cagttttttc tttggcaagg tctgtacgag caaggtctga tttgagactt gcttgacgtt
    20821 tgtagtcagc cacggcttct gcctggtcag ctgttgtaat agcatcaacc aaggcatgct
    20881 caggagcaag aaccgcgtaa gttgcaccaa aaagggtgtc aggacgtgtt gtaaagacag
    20941 tgaagtcctt atctgtatcc ttaaccttga aggttacatt agcaccagtt gatttaccaa
    21001 tccagttgcg ttgcatgtcc ttgattgatt ctggccagtc aacttcttcc aagtcttcta
    21061 gcaagcgctc tgcataggca gtgattttga gcatccattg gcgcatgggt tttctgacaa
    21121 ctgggtagcc gccacgttct gatgtcccat cagggagcac ctcttcgttg gcgatagctg
    21181 ttcccaattc ttcaacccag ttaacgggaa cttcagcttc gtaggctagg cctttttcat
    21241 aaagcttggt aaagatccac tgcgtccact tgtagtagtt agggtcagtt gtgttaactt
    21301 cacgatccca gtcgtatgag aaaccaagag cattaatctg gcgtttgaag ttagcgatgt
    21361 tttcagctgt aaactcggct gggtcattac cagtatccat agcgtactgc tcagcaggaa
    21421 gtccaaaggc atcccaaccc attgggtgga ggacattgtg accttgggca cgtttgaaac
    21481 ggctgagaat atccgttgct gtatagcctt ctgggtgacc aacgtggaga ccagctcctg
    21541 atgggtatgg gaacatatct agggcataaa acttaggttt tgatgcgtct gttcctgttt
    21601 taaaagtatg attatctgcc caaaaggctt gccatttggg ttcaatagcg gtgtgatcgt
    21661 aaaatgtcat gtgaaatctc ccatgtaaaa aatctagtct tcttattata gcatgagatg
    21721 aagcttttag gaatggtcaa gacatgacta gcaccttggc ttttgagact tcagacgatt
    21781 agctattttt aaaccctgct ttagcatcaa ccatcctcac aaaaagctaa taaaaaaagc
    21841 cagacttgct ctgtaaacag agccaatctg gacaaataga cttaaaaagc aagtgcttgc
    21901 tcaaaatcgg ccaataaatc ttcgatattt tcaacaccac aagacaagcg cagcaaacca
    21961 tcaacaaccc cttgttccaa ccgcgcttgt gatgataaac aagcatgaga catcgttgct
    22021 ggatgtgaca aaatggattc tactccacct agactaacgg ctaaaatcgg caactgaata
    22081 cgatgggtaa aggtcatcag ctcctcctta gaagccaact caaaggataa aacagcacca
    22141 ccattcttag cttgttgcag gtgggtatcg tgattaggat gactgtctaa gccagggtaa
    22201 tgtaccttaa gaactttggg gtgcttcgct aaatagttag cgagctgctg agcatttttt
    22261 accgcttgtt ccatacggat ccccatagtt ttcatgccac gaagaatcag ccaagcgtct
    22321 tcgaccccaa caattgctcc aaagtttttt tgaaaaagct tgagctggtt gtagatagcc
    22381 tcattattgg tagcagctaa accagctacc acatcactat gaccattgat aaatttggtc
    22441 acactttcta ccacaatatc cacacccata gctaagggtt cttgatacaa acttgtcata
    22501 aaggtattat cagccacagt tagcacacca taagctttgg cgagttgcac aagctctgaa
    22561 atatcacaaa ttttcaagag cggatttgac ggtgtctcta gataaatcat acgcgtatta
    22621 ggcctgatac ttgcttttac tgttgctaaa tcagccatat ctacaaagct tgtttcaatc
    22681 tgataattgg gtaaaatagc tgtcgcaaat tgacaagtcc ctccatagac ctccaatggg
    22741 aaaatcacat gatctccagc ttttaagagc atcaaaacag ttgaaatagc agccatacca
    22801 gatgcataag ccaccgcata agtcgctttt tctaaacagg ctaacccgtc ttctaaagct
    22861 tcagtcgttg ggtttgaaaa gcgtgtgtac aaatgttttt gactaggaca atacagttct
    22921 gaattgtgga acgtcgaagt ctgatagatg ggaactgaag ctgctcctgt aaactcgtca
    22981 ataaccgtat agccatgtaa taaagtagtg ttttctttca tttcgtgcac ctccgcaata
    23041 gcgttgactc caatgaactc tcttttagac aatggtccca ccccaatggt tgcacaagcc
    23101 aaaaagctct tcaaaaaagc gtgatctgct aacctataag aaaagtagtg ggcacctttt
    23161 aaacggataa tgaatttttc caacaatatc atttttagat cgattacgtt tattatacaa
    23221 cctttagcta agtttgcaag cccttttatc atctttgacc aagctgccaa cacctctcca
    23281 gcaagttaaa gtatagctac ctttggtccc atcgtctcaa aagcttagct tatcacactg
    23341 acagtcctac tttgcatcgt cacctactca atacggcaat attgccatca aaatttcatt
    23401 aaaaggtcac aatttatttc atttaatgag aaacagaact tgttttcacc atgcgtctta
    23461 tagtacactg ctaaagtact atttattttt aggagagaaa tatagtgaaa aacaaattat
    23521 ttttagttgc cctcgcgacc gtaaccgtcc tagggccgtc tttagcaacc cctcatcacc
    23581 agaccgtgca tgcgactgag caggcaaaag aacctcagac caactcaatg acgtgtacta
    23641 acagtggagc tgtatgcgct ggctggcata gcttaacaga agaagaagac agtataactt
    23701 caaattcatc ttcaaaaacc aatacttccc cgttagcttg gttatggagc ctaataacaa
    23761 gtttgtggat ttctgcaaca agcttgttca cctaagatct ctataatcat atccatggaa
    23821 tttaaataac catctcgtgg cggttttcta atcctaggtc gccacatttc ttattcagat
    23881 aaataataat aaaaacatta aaataatact tgatttatga acaaaaagtt agtataatat
    23941 aatcacaata caaattaaag gagttatcta tgaaaaagaa attatcttta tttatgatcg
    24001 caactgctgc tgttgcaggc cttagccttg caacactagg aagccaccaa actgtttcag
    24061 caaacgatgc tagctatagt aacggtttct acggacacaa tggtcctgac agctggttac
    24121 tttacacagt atggtaaacc tcataccgta atatagaagc tatcttcttc gtaagactac
    24181 ggttatgaaa agcctaaggc tgcataagca gccttaggct ttttgattgg ctaaaacgtt
    24241 tttgccaatg acacctcaag gtaaaacaga aaaggcacac tgcctataaa aggagagaag
    24301 cccaaaacac aagtcgtaac agttgctagc taaccagcac caaaaaagct gtaacacctc
    24361 aaatagaatg ataaccagat taatattatt agtaattaat gtgatttata taagataaaa
    24421 aatactttag tcttggcttt ctttgccttt ttcggtcaaa cacatcacaa aaaga
//
LOCUS       DATXIG010000020        22381 bp    DNA     linear   BCT 28-DEC-2023
DEFINITION  TPA_asm: Streptococcus pyogenes strain MGAS11113
            SAMN08555799-rid19682583.denovo.020, whole genome shotgun sequence.
ACCESSION   DATXIG010000020 DATXIG010000000
VERSION     DATXIG010000020.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN08555799
            Sequence Read Archive: SRR6740495
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pyogenes
  ORGANISM  Streptococcus pyogenes
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 22381)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 22381)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-OCT-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-OCT-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001918058.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001918058.2
            Genome Coverage       :: 368x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/12/2023 11:23:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 1,806
            CDSs (total)                      :: 1,769
            Genes (coding)                    :: 1,722
            CDSs (with protein)               :: 1,722
            Genes (RNA)                       :: 37
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1, 1 (5S, 23S)
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 47
            CDSs (without protein)            :: 47
            Pseudo Genes (ambiguous residues) :: 0 of 47
            Pseudo Genes (frameshifted)       :: 30 of 47
            Pseudo Genes (incomplete)         :: 20 of 47
            Pseudo Genes (internal stop)      :: 9 of 47
            Pseudo Genes (multiple problems)  :: 11 of 47
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..22381
                     /organism="Streptococcus pyogenes"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN08555799-rid19682583.denovo.020"
                     /strain="MGAS11113"
                     /serotype="Emm28"
                     /isolation_source="normally sterile site"
                     /host="Homo sapiens"
                     /db_xref="taxon:1314"
                     /country="Finland"
                     /collection_date="2002"
                     /collected_by="National Public Health Laboratory"
     gene            complement(234..383)
                     /gene="rpmG"
                     /locus_tag="VC122_000869"
     CDS             complement(234..383)
                     /gene="rpmG"
                     /locus_tag="VC122_000869"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001265622.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L33"
                     /protein_id="HEP5640806.1"
                     /translation="MRVNITLEHKESGERLYLTSKNKRNTPDRLQLKKYSPKLRKHVT
                     FTEVK"
     gene            complement(399..581)
                     /gene="rpmF"
                     /locus_tag="VC122_000870"
     CDS             complement(399..581)
                     /gene="rpmF"
                     /locus_tag="VC122_000870"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018380522.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L32"
                     /protein_id="HEP5640807.1"
                     /translation="MAVPARHTSKAKKNKRRTHYKLTAPSVQFDETTGDYSRSHRVSL
                     KGYYKGRKIAKANEAK"
     gene            801..2081
                     /locus_tag="VC122_000871"
     CDS             801..2081
                     /locus_tag="VC122_000871"
                     /EC_number="6.1.1.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011889242.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine--tRNA ligase"
                     /protein_id="HEP5640808.1"
                     /translation="MKLQKPKGTQDILPEDAAKWQYVESVARDTFSQYNYGEIRTPMF
                     EHYEVISRSVGDTTDIVTKEMYDFYDKGDRHITLRPEGTAPVVRSYVENKLFAPEVQK
                     PVKLYYIGSMFRYERPQAGRLREFHQIGVECFGAANPATDVETIAMAYHLFEKLGIKD
                     VTLHLNSLGSPESRSAYRQTLIDYLTPMRDQLSKDSQRRLDENPLRVLDSKEKEDKLA
                     VEKAPSILDYLDEESQAHFEAVKDMLEALDIPYVIDTNMVRGLDYYNHTIFEFITSVE
                     GSDLTICAGGRYDSLVGYFGGPETPGFGFGLGLERLLMIIEKQGITLPIETEMDVYLA
                     VLGDSANSKALELVQAIRRQGFTAERDYLGRKIKAQFKSADTFKAKLVMTLGESEVEA
                     GKAVIKNNRSRQEVEVSFEDMMTNFANISEQLLS"
     gene            2419..4167
                     /gene="aspS"
                     /locus_tag="VC122_000872"
     CDS             2419..4167
                     /gene="aspS"
                     /locus_tag="VC122_000872"
                     /EC_number="6.1.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003061903.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate--tRNA ligase"
                     /protein_id="HEP5640809.1"
                     /translation="MKRSMYAGRVREEHIGTTITLKGWVSRRRDLGGLIFIDLRDREG
                     VMQLVINPEEVSSDVMATAERLRSEYVIEVEGFVEARQQANDKLATGMVELKVSALTI
                     LNTAKTTPFEIKDDVEVSDDTRLRYRYLDLRRPEMLENFKLRAKVTHSIRNYLDDLEF
                     IDVETPMLTKSTPEGARDYLVPSRVSQGHFYALPQSPQITKQLLMNAGFDRYYQIVKC
                     FRDEDLRGDRQPEFTQVDLETSFLSEQEIQDIVEGMIAKVMKETKEIDVTLPFPRMSY
                     DVAMNSYGSDKPDTRFEMLLQDLTVTVNGIDFKVFSEAPAVKAIVVKGNADRYSRKDI
                     DKLTEFAKQFGAKGLAWVKVTDGQLAGPVAKFLIAIETELSSQLKLAENDLVLFVADT
                     LEVANNTLGALRNRIAKDLDMIDQSQFNFLWVVDWPMFEWSEEEGRYMSAHHPFTLPT
                     PESAHELEGDLAKVRAIAYDIVLNGYELGGGSLRINQKEMQERMFKALGFTADEANDQ
                     FGFLLEAMDYGFPPHGGLAIGLDRFVMLLAGKGNIREVIAFPKNNKASDPMTQAPSLV
                     SENQLEELSLQIESHD"
     gene            4160..5101
                     /locus_tag="VC122_000873"
     CDS             4160..5101
                     /locus_tag="VC122_000873"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011185085.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YitT family protein"
                     /protein_id="HEP5640810.1"
                     /translation="MIKKTTYKKKVKYVISRGAKKVGLLHALRSISREKYAEKISASL
                     LYGILSSIAVNFFFQPGHVYSSGATGLAQVFSALSHRLLGYDFPIAFAFYLINIPLLI
                     LAWYKIGHQFTIFTFITVSMSSFFIQIMPQVTLTTDPLINAIFGGLVMGMGIGTGLKS
                     RISSGGTDIVSLTLRKRTGKDVGSLSLMVNGAILAFAGILFGWQYALYSMVSIFVSSR
                     VTDAIFTKQKKMQATIVTSHPERVIHMIHKRLHRGVTSINDAEGTYKHEQKAVLITIL
                     TCEEYPEFKWLMLKTDPQAFVSVAENVRIIGRFVEDD"
     gene            5121..5999
                     /locus_tag="VC122_000874"
     CDS             5121..5999
                     /locus_tag="VC122_000874"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002991372.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YitT family protein"
                     /protein_id="HEP5640811.1"
                     /translation="MSIDQRLQLKKFCFIILGAAIYAFAFVYFYMANRIAANGLAGLT
                     LVGKALFEIDPSLIGYLINLPLVLLGARCFGKRAMVYTVTGVLSLYFFVWLFQRFPLV
                     VDLDHDNLVVSLMAGIIGGLGGGFVFRNGGTIGGADIIAKLLEDKLGLQLNQALLGID
                     LFVMIVSLTYISLPQMMYALIASFMYGQIVRLVQQGGYSARGIFIVSEQAEKIARFIM
                     EELGRGVTYLTGEGAYSGRRKKVIYVALGPRDIRELKAFLTQVDPNAFITYFDVNEVN
                     SPEFLTIKSKYHKKRK"
     gene            6042..6914
                     /locus_tag="VC122_000875"
     CDS             6042..6914
                     /locus_tag="VC122_000875"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011185083.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YitT family protein"
                     /protein_id="HEP5640812.1"
                     /translation="MAHHDKLTKLLKLFLIALGVAIYTFGFVNFNMANALAEGGVAGI
                     TLILHAHFGINPAYSSLLFNLPLFILGAKIFGKRSLALTIYGTVLMSAFIWMWQKVPI
                     ELGLENDMMLVAVVAGLFSGIGSGIVFRYGATTGGTDIIGRIAEEKFGAKLGQTLLLV
                     DALVLTASLTYVDLKHMLYTLVASFVFSQMISVVQNGGYTIRGMIIITKHSEAAAQAI
                     LTEINRGVTYLKGQGAYSGNDYNIMYVTLNPTEVREVKRILAGLDPDAFISIIDVDEV
                     ISSDFKIRRRNYDK"
     gene            complement(6941..7249)
                     /locus_tag="VC122_000876"
     CDS             complement(6941..7249)
                     /locus_tag="VC122_000876"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011889238.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bacteriocin immunity protein"
                     /protein_id="HEP5640813.1"
                     /translation="MPSEKEILDALSKVYSEQVIQADDYFRQAIFELASQLEKEGMSS
                     LLATKIDSLINQYILTHQFDAPKSIFDLSRLVKTKASHYKGTAISAIMLGSFLSGGPK
                     "
     gene            complement(7337..9028)
                     /locus_tag="VC122_000877"
     CDS             complement(7337..9028)
                     /locus_tag="VC122_000877"
                     /EC_number="6.1.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015632348.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arginine--tRNA ligase"
                     /protein_id="HEP5640814.1"
                     /translation="MDTKTLIASEIAKVVPELEQDAIFNLLETPKNSDMGDLAFPAFS
                     LAKVLRKAPQMIASELAEQIDESQFEKVVAVGPYINFFLDKTKISSQVLEQVITAGSD
                     YAQQDEGQGRNVAIDMSSPNIAKPFSIGHLRSTVIGDSLAHIFAKMGYKPVKINHLGD
                     WGKQFGMLIVAYKKWGDEAAVQAHPIDELLKLYVRINAEAETDPTVDEEAREWFRKLE
                     DGDKEATELWQWFRDESLLEFNRLYDQLHVTFDSYNGEAFYNDKMDEVLDLLEAKNLL
                     VESKGAQVVNLEKYGIEHPALIKKSDGATLYITRDLAAALYRKRTYDFAKSVYVVGNE
                     QAAHFKQLKAVLKEMGYDWSDDMTHVAFGLVTKGGAKLSTRKGNVILLEPTVAEAINR
                     AASQIEAKNPNLADKEAVAHSVGVGAIKFYDLKTDRMNGYDFDLEAMVSFEGETGPYV
                     QYAHARIQSILRKADFTPSATTTYSLADAESWEIIKLIQDFPRIIKRTSDNFEPSIMA
                     KFAINLAQSFNKYYAHTRILDDNSERDNRLALCYATATVLKEALRLLGVDAPNEM"
     gene            9319..9756
                     /gene="argR"
                     /locus_tag="VC122_000878"
     CDS             9319..9756
                     /gene="argR"
                     /locus_tag="VC122_000878"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002982171.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arginine repressor"
                     /protein_id="HEP5640815.1"
                     /translation="MNKMERQQQIKRIIQAEHIGTQEDIKNHLQKEGIVVTQATLSRD
                     LREIGLLKLRDEQGKLYYSLSEPVATPFSPEVRFYVLKVDRAGFMLVLHTNLGEADVL
                     ANLIDNDAIEDILGTIAGADTLLVICRDEEIAKRFEKDLAAGL"
     gene            9753..10105
                     /locus_tag="VC122_000879"
                     /pseudo
     CDS             9753..10105
                     /locus_tag="VC122_000879"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014623648.1"
                     /note="frameshifted; internal stop; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="RicAFT regulatory complex protein RicA family
                     protein"
     gene            10092..12647
                     /gene="mutS"
                     /locus_tag="VC122_000880"
     CDS             10092..12647
                     /gene="mutS"
                     /locus_tag="VC122_000880"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011018318.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA mismatch repair protein MutS"
                     /protein_id="HEP5640816.1"
                     /translation="MAKTNISPGMQQYLDIKKDYPDAFLLFRMGDFYELFYEDAVKAA
                     QLLEIGLTSRNKNAENPIPMAGVPHHSAQQYIDVLIELGYKVAVAEQMEDPKQAVGVV
                     KREVVQVITPGTVVDSAKPDSANNFLVAVDFDGCRYGLAYMDVSTGEFCVTDLADFTS
                     VRSEIQNLKAKEVLLGFDLSEEEQTILVKQMNLLLSYEETVYEDKSLIDGQLTTVELT
                     AAGKLLQYVHKTQMRELSHLQALVHYEIKDYLQMSYATKSSLDLVENARTNKKHGSLY
                     WLLDETKTAMGMRLLRSWIDRPLVSKEAILERQEIIQVFLNAFIERTDLSNSLKGVYD
                     IERLSSRVSFGKANPKDLLQLGHTLAQVPYIKAILESFDSPCVDKLVNDIDSLPELEY
                     LIRTAIDPDAPATISEGSIIRTGFDERLDHYRKVMREGTGWIADIEAKERQASGINNL
                     KIDYNKKDGYYFHVTNSNLSLVPEHFFRKATLKNSERYGTAELAKIEGQMLEAREESS
                     SLEYDIFMCIRAQVETYINRLQKLAKILATVDVLQSLAVVAETNHYIRPQFNDNHVIT
                     IQEGRHAVVEKVMGVQEYIPNSISFDQQTSIQLITGPNMSGKSTYMRQLALTVIMAQM
                     GSFVAADHVDLPLFDAIFTRIGAADDLISGQSTFMVEMMEANQAIKRASDNSLILFDE
                     LGRGTATYDGMALAQAIIEYIHDRVGAKTIFATHYHELTDLSTKLTSLVNVHVATLEK
                     DGDVTFLHKIAEGPADKSYGIHVAKIAGLPKSLLKRADEVLTRLETQSRSTEIISVPS
                     QVESSSAVRQGQLSLFGDEEKAHEIRQALEAIDVMNMTPLQAMTTLYELKKLL"
     gene            complement(13044..13697)
                     /locus_tag="VC122_000881"
     CDS             complement(13044..13697)
                     /locus_tag="VC122_000881"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014407949.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEP5640817.1"
                     /translation="MWSSIFNFKENIVKKKLVMMLTLLISICAISVRANESGDLFTTI
                     GQSHLPGVTVAPFKTGDTTINVNLDPYGYVYGFVTSSNGNKIKKTENKTYEIPCSILE
                     KQDNPNCEGRKWTVSGNRADGGGNYTVQLEKPLAEGDKVTLSFADDGNLYFGQLVFED
                     GKTHNERLQKERDAEEQQYAEVLFKRSIEEESKKTWHQRLSDSIQDQWWNFKDWLRG"
     gene            complement(13862..14245)
                     /locus_tag="VC122_000882"
     CDS             complement(13862..14245)
                     /locus_tag="VC122_000882"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003058801.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ArpU family transcriptional regulator"
                     /protein_id="HEP5640818.1"
                     /translation="MSKKNAIRKLKEFHRWQRIANSLDLTYTELYQFDIEYHPTRRKH
                     LEISRECALEELDAIRYAINQLSKVEYRQILIECYLISEEKTQQDIMEELNRSQSWYY
                     ESKKRALLEFVEFYRDGALKNNVRL"
     gene            complement(14220..14582)
                     /locus_tag="VC122_000883"
     CDS             complement(14220..14582)
                     /locus_tag="VC122_000883"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011889234.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1492 domain-containing protein"
                     /protein_id="HEP5640819.1"
                     /translation="MSAKEQLKELKPLFALITLFEEQRDKDIELINAFHNPEAIRHIE
                     KGTAKQLLYLAKERDKRLAMIATLQDERQIAVIKARYVDDLSWDEILDKLGYSRNIVF
                     KLHREALEVLDEQEERYS"
     gene            complement(14986..15474)
                     /locus_tag="VC122_000884"
     CDS             complement(14986..15474)
                     /locus_tag="VC122_000884"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000891150.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEP5640820.1"
                     /translation="MLELSIENIIKPMKTQGKTRVTGSIGDQAIRIDLDGLVIHYNGQ
                     GLLLETIPGTYGGKRYFFVCPDCERRCRKLFKASHAFACGSCQKVHQATLNRSKTDCC
                     YYWQLAFKECLKVNPEARHIHGYYSRDDFPKRPKYMRLTKYLYHWRRFHYYMDKGDRY
                     WL"
     gene            complement(15548..16057)
                     /locus_tag="VC122_000885"
     CDS             complement(15548..16057)
                     /locus_tag="VC122_000885"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001258765.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEP5640821.1"
                     /translation="MRTFSDTPKTFTFHYTFKDFDTAQVACHAILGYMTGTYEQPVID
                     ATYHNDDQGGHANQLVLKYVEDRKLNKVFKRICDSFKDYYNQPEDMTDEELDHQRVVS
                     LSKSTQGKVNNRDTLIAFISDHNQLAEHLSMNYKEMTPEDLGAILESISQAFNHLYDM
                     VVEGQLLVK"
     gene            complement(16059..16232)
                     /locus_tag="VC122_000886"
     CDS             complement(16059..16232)
                     /locus_tag="VC122_000886"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001873750.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEP5640822.1"
                     /translation="MKLKLHTRGGNTITIQGDRTLYNELVKYLLSGEQPNWVASPSAI
                     INLSDIIAITKEK"
     gene            complement(16238..16411)
                     /locus_tag="VC122_000887"
     CDS             complement(16238..16411)
                     /locus_tag="VC122_000887"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000694572.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2758 domain-containing protein"
                     /protein_id="HEP5640823.1"
                     /translation="MKIKLFYQKHNESLDDFEHRVNLFTLSVSVIDIKFQEATYGNYE
                     DMDSRTGLLVLYR"
     gene            complement(16408..16662)
                     /locus_tag="VC122_000888"
     CDS             complement(16408..16662)
                     /locus_tag="VC122_000888"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001069293.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEP5640824.1"
                     /translation="MNPNYTNHGVKKMRYNYDDLPNDQLYRLELPNGQCGYDFITKIN
                     FNIKSRPGDCDFTRPELTRRGYQWVFDCGFAIPVEKGAKT"
     gene            complement(16956..18350)
                     /locus_tag="VC122_000889"
     CDS             complement(16956..18350)
                     /locus_tag="VC122_000889"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000656427.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="virulence-associated protein E"
                     /protein_id="HEP5640825.1"
                     /translation="MKDYQNKLSQATQPAFAPAFRTRKGRGDKEYVISSPYNVGKVFE
                     FYENIYTGIKYNEFEKTIEITKAVPWSKEKGLWTNEQTSLCIAFIDEKYRFTPRKEHI
                     EVAITALAKKNTYHPIKQRIESQKWDGKARGERYFIDLLGCADNSYNREIAKVWLTGL
                     MARIYLRKVKFEVVPILIDKRQGTGKSTVTKRLLPSYHTDSEIKFGKNDSDYQKIQAN
                     AIIELGELKGMSKAEIETVKSFISSDSDTYREPYERKATPHPRHCVFIGTANKKSFLK
                     DSGTERRFFPIECGINDVKKHPMEVEEDYFLQVLAEAKVWFNNYEPLTPSKELMNQLA
                     DIQEDYKVEDVDKEIIDQLLNEFQIVEGWDSLSQYEQRQYILKQLGEPLDNAQSYSDY
                     PSAQTDCLLQVTSPNHIAYLGFNQKPTQGGKALISQKIRDYLDNDDGWKKGENPARKR
                     LFKGGTPVPYYERV"
     gene            complement(18362..19228)
                     /locus_tag="VC122_000890"
     CDS             complement(18362..19228)
                     /locus_tag="VC122_000890"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001029301.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="primase alpha helix C-terminal domain-containing
                     protein"
                     /protein_id="HEP5640826.1"
                     /translation="MAIYEARGFSSYLYPYKGPLEPFDYVAQFRPLKPPEDIDIEEYK
                     RTQAPYCLSGKVTAEKNGSYKRNNASLVYRDLIFLDYDEIETGVNLPKIVSQTLWEYS
                     YIIYPTIKHTPEKPRYRLVMKPSDVMTEATYKQVVKEIADKIGLPFDLASLTWSQLQG
                     LPVTTGDPEDYQRYVNHGLDYPVPKNGSTPNRQVVTTYTPRPRSQRSITMRVIDTLFN
                     GFGDEGGRNVALTKFVGLLFNKWVDCDLETAYELVQIANSVTTKPLPIDEIDTTFRSI
                     LDKELRKRGIKP"
     gene            complement(19229..19501)
                     /locus_tag="VC122_000891"
     CDS             complement(19229..19501)
                     /locus_tag="VC122_000891"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014407941.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEP5640827.1"
                     /translation="MNVFSQDYEAKLLEQNLTAFNHFLEAYQKPKPRVLGLITAEQVK
                     EELNIKGKTLKRWENAGLRRYQPPLEDTRKHYYKVSDILIFLGVNV"
     gene            complement(19504..19833)
                     /locus_tag="VC122_000892"
     CDS             complement(19504..19833)
                     /locus_tag="VC122_000892"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000174495.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEP5640828.1"
                     /translation="MTLPENYRRVLNLIKVGADNPITGAEISLILKLEERSVQSIISS
                     LITRYNVPIIGIRHGFNRGYFIPANKEELLDGAKAFYNQVQKEQERLSVLLNADLTSY
                     KELLKGG"
     gene            complement(19845..20036)
                     /locus_tag="VC122_000893"
     CDS             complement(19845..20036)
                     /locus_tag="VC122_000893"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017645222.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEP5640829.1"
                     /translation="MQELNLTPTQTLILFFVLGLLGLLLSRSKPLIEIDLPEDTHAPK
                     PRQNANYGAYIQAQNHYYN"
     gene            complement(20036..20368)
                     /locus_tag="VC122_000894"
     CDS             complement(20036..20368)
                     /locus_tag="VC122_000894"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002992497.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEP5640830.1"
                     /translation="MKYRVETNPFSKDRYTPEQLEMFKNRQLSKDKAEVYFTRLYNQH
                     IAWVIIANVMTEYVIKFRKSATSFEEAWDALDYQRTTEIVFRAVNGLPCSEKDSGELE
                     TYLSEVSA"
     gene            complement(20470..20922)
                     /locus_tag="VC122_000895"
     CDS             complement(20470..20922)
                     /locus_tag="VC122_000895"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000916929.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEP5640831.1"
                     /translation="MLKNQDNMIKKKRNPRVLFRNVAYKLSEIEGKTLTEIASFLGFG
                     NSEVCRSTLYKWKRKKWLKFDLKNGHYRNVEVLHEVTLEKMANKELKEQGLIYKANIY
                     YEQVVSTSEIIEDIKTKTQDRIKAIHLQQKALERIPSELFAELYTNMN"
     gene            complement(21063..21236)
                     /locus_tag="VC122_000896"
     CDS             complement(21063..21236)
                     /locus_tag="VC122_000896"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000074663.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CopG family transcriptional regulator"
                     /protein_id="HEP5640832.1"
                     /translation="MSPKMGRPVKGTAKRDKRLEVRLTADEYNTIQETADKNGLSKAD
                     LIVKAVNSYESEK"
     gene            complement(21454..21627)
                     /locus_tag="VC122_000897"
     CDS             complement(21454..21627)
                     /locus_tag="VC122_000897"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000132665.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CopG family transcriptional regulator"
                     /protein_id="HEP5640833.1"
                     /translation="MTDTKKRVGRPKGENSNTVKLTVRVSEELDSKLNKYAKENNLTK
                     PETLRKAFENLTK"
     gene            complement(21875..>22381)
                     /locus_tag="VC122_000898"
     CDS             complement(21875..>22381)
                     /locus_tag="VC122_000898"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011185071.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rha family transcriptional regulator"
                     /protein_id="HEP5640834.1"
                     /translation="EAFGVLSFEIHKPEKGSLGGRPRRIYHLNEQQATLLVTYLGNTE
                     PVREFKKNLVKAFFEMREELTQIRLQRSLEAPKRKTLNEAIKTWEHAPKMAYPTVYNL
                     LLKAVTGKNSKQLKVTRGGYSDIDCLNSIELAQYTALEDMAIALINLNFTYQDIKTMA
                     LKNTLQCA"
BASE COUNT         6338 a         4051 c         4542 g         7450 t
ORIGIN      
        1 aatttcagct acaccaaaat atagataaga agttagtgta ccaaatataa aaaagccctg
       61 ccatccaaat gataacaggg cttaacttca atatccaaat gatatattca tctaaaaaag
      121 gtagagtaaa aggtagagtt cttattgatt tatattacaa tgtaatgaac ttcaaaaatt
      181 caaaaatcgc ttaaaatcaa cgttttgacg tctaatgacg tgtagtgaac cccttactta
      241 acctctgtaa atgtaacgtg tttacgtaat tttggtgagt attttttcaa ttgaagacgg
      301 tctggagtgt tacgtttgtt ttttgaagta aggtacaagc gttcaccaga ttctttatgt
      361 tcaagtgtaa tatttacgcg catggtatct cccttctatt atttagcttc gttagcttta
      421 gcgattttac gtcctttgta gtatccttta agggatacac ggtgagaacg tgagtaatct
      481 ccagtagttt cgtcgaattg tacagatgga gctgtcaatt tgtagtgtgt acgacgtttg
      541 tttttcttcg cttttgacgt gtgacgtgca ggtactgcca ttttcttatt cttcctttcg
      601 atgtgtaagt tttgattttc atcaacttca ctatgataac atatcttttt tgtaaagtaa
      661 agttacttga caaaactttt tatttttaca atttcatggt gaaaatacta tcaaactaaa
      721 gcaaatgata tatgcttgtt taaaatataa gaaaaaatgg tagaatgttt aaagagaaaa
      781 ctaatcaaag aggtaaactt atgaaacttc aaaaaccaaa aggaacccag gatattcttc
      841 ctgaggatgc tgcgaaatgg cagtatgtgg aatctgttgc gcgtgatacg tttagccaat
      901 acaattatgg agaaattcgt actcctatgt ttgaacacta cgaggtaatc tcgcggtctg
      961 tgggtgatac gactgatatt gtgacgaagg aaatgtatga tttctatgat aaaggagatc
     1021 gtcacattac cttgcgacca gagggtacag ctccagtggt acgttcttat gttgaaaata
     1081 aattgtttgc gccagaggtc caaaaacctg tcaaacttta ttatatcggt tccatgttcc
     1141 gctacgaacg ccctcaagct ggtcgtttac gtgaatttca ccaaattgga gtagaatgtt
     1201 tcggtgctgc taatccagca acagatgttg aaaccattgc gatggcctat cacctctttg
     1261 aaaaattggg tattaaggat gtcacccttc atttaaatag tttggggagc cctgagagtc
     1321 gttcagctta tcgtcaaacc ttgattgact acttaacacc aatgcgtgat caattatcta
     1381 aagatagtca acgtcgcttg gatgaaaacc cactgcgcgt gcttgattct aaagaaaaag
     1441 aagataaact agccgttgaa aaggcgcctt ctattttgga ttacttagat gaggaaagtc
     1501 aggcgcactt tgaggcggtc aaagatatgc ttgaagcact tgacattcct tatgtgattg
     1561 acaccaatat ggtacgtggc cttgattact ataaccacac tatttttgaa ttcatcacgt
     1621 ctgtggaggg ctctgactta accatctgtg cgggtggacg ttatgatagt ttggtaggtt
     1681 attttggtgg accagaaacc cctggctttg gctttggtct tggtttagaa cgtctcttga
     1741 tgattattga aaagcagggc attaccttgc caattgagac agagatggat gtttaccttg
     1801 ctgtgttagg tgatagtgct aatagcaaag ctttagaatt ggttcaagcc attcgccgtc
     1861 aagggtttac agctgaacga gattaccttg gccgtaagat taaggctcag tttaaatcag
     1921 ccgatacctt taaagcaaaa ttggtaatga ctttaggaga aagtgaagtt gaagcaggca
     1981 aagctgtgat taagaataat cgtagtcgtc aagaagtgga agtcagtttt gaagatatga
     2041 tgacaaactt tgcaaacatc tcagagcaat tattgtctta atgattttat tgtcgtcata
     2101 agcttttgtt gatatgattg tttagaagat aatcactaaa acaggtgtaa cttccctttt
     2161 tagaagggag ttgcacctgt ttgttttctg taaggggatt gattagcttt cttagtattc
     2221 tgtgaggaca cctgatatgt caaaatgctg acgactcagt attcttgctt tatctttaga
     2281 aaaactcttt ttaaatgggt ggggttttta agggcttttt gctataatag tagggaatga
     2341 attaattgat ttatatagtg aaaggaattg gaaacgagca ggtttcacag gattctgttt
     2401 gttttaggga atttatttat gaaacgtagc atgtatgcag ggcgtgttcg cgaagaacac
     2461 attggaacga caatcacatt gaaaggatgg gtcagtcgtc gtcgtgactt gggtggtctc
     2521 atctttattg atttacgtga ccgtgagggt gtgatgcaat tagtcattaa ccctgaggaa
     2581 gtttccagtg atgtgatggc aactgctgaa cgcctacgta gtgaatatgt gattgaagtg
     2641 gaaggttttg ttgaagctcg acaacaagcc aatgataagt tggcgacagg aatggttgag
     2701 ttaaaagtat ctgctttgac cattttaaac acagctaaaa cgacgccttt tgaaattaaa
     2761 gatgacgttg aagtaagtga tgacacgcgt ttacgttacc gttacttaga tttacgacgt
     2821 ccagaaatgt tagaaaactt caagctacgt gcaaaagtga ctcattctat ccgtaattac
     2881 cttgatgacc ttgagtttat tgatgtcgag acacctatgt tgacaaaatc aactccagaa
     2941 ggtgcgcgtg actatctggt accaagtcgt gttagccaag ggcatttcta tgctttgcca
     3001 caaagtccgc aaattaccaa acagttattg atgaatgctg gctttgatcg ctattatcaa
     3061 attgtgaaat gtttccgcga tgaagatcta cgtggtgacc gtcaacctga gtttacacaa
     3121 gttgacttgg aaacgtcatt cctttctgaa caagagattc aagacatcgt tgaaggcatg
     3181 attgctaaag tgatgaaaga gacaaaagaa atagatgtaa ccctaccatt cccacgtatg
     3241 agctacgatg tagcaatgaa tagctacggc tctgataaac cagacacacg ttttgagatg
     3301 cttttacaag acttgacagt aactgtcaat ggaattgact tcaaggtttt ctcagaagca
     3361 ccagctgtca aggcgattgt ggtgaaggga aatgcagatc gatactctcg taaagatatt
     3421 gacaaattaa ctgagtttgc caaacaattt ggtgctaaag gccttgcctg ggttaaggtg
     3481 acagatggtc aattggcagg cccagtagcc aagttcttga tagctattga gacagagttg
     3541 tcaagccaat taaaacttgc tgagaatgat ttggttttat ttgtagcgga tactcttgag
     3601 gttgctaata atacattagg tgcgcttcgt aatcgtattg ccaaggacct tgacatgatt
     3661 gatcagtcgc agtttaattt cctttgggtg gttgattggc caatgtttga gtggtcagaa
     3721 gaagaaggac gttatatgtc agctcatcac cccttcactc ttccaacgcc agaatctgct
     3781 catgagttag agggtgattt ggctaaggta cgggcaattg cttatgacat cgttttaaat
     3841 ggttacgagc ttggtggtgg tagcctgcgt atcaatcaaa aagagatgca agagcgcatg
     3901 ttcaaggctc ttggctttac cgctgatgaa gctaacgacc aatttggttt cttactagaa
     3961 gcgatggact acggctttcc tcctcatggt ggcttggcaa ttggtcttga ccgttttgtc
     4021 atgttgcttg ctggaaaagg caatattcga gaagttattg ccttccctaa aaataacaaa
     4081 gcctctgatc caatgacgca agcacctagt cttgtttctg aaaaccaatt agaagaactc
     4141 agtcttcaaa tagaaagtca tgattaagaa aacaacctat aagaaaaaag ttaaatatgt
     4201 cattagtaga ggggccaaga aagttggcct actccacgct ctaagaagta tttcaagaga
     4261 aaaatatgca gagaagattt cggcttctct gctttatggc attctctcta gtattgctgt
     4321 gaattttttc ttccagcctg ggcatgttta ttcaagtgga gcaactggtc tagcacaggt
     4381 tttttcagct cttagtcatc gtcttttagg ctatgatttt cccatcgcct ttgcgtttta
     4441 tttgattaat attcctttgc ttattttagc ttggtataaa attgggcatc aatttaccat
     4501 ttttaccttt atcacagtca gcatgagttc tttctttatt caaatcatgc ctcaagtgac
     4561 gctgacgact gatcctctta tcaatgctat ttttggtggt ttggttatgg gaatgggaat
     4621 tggtacaggt ctcaaatcac gtatctctag tggggggact gatattgtca gtttgaccct
     4681 tagaaaacga acaggcaagg atgtgggcag tctctcattg atggttaatg gtgcaatttt
     4741 agcctttgca gggattttat ttggctggca gtacgccctt tattctatgg tctctatctt
     4801 tgtatcaagt cgtgttacgg atgccatttt caccaagcaa aagaaaatgc aggcaactat
     4861 tgttaccagc catccagagc gtgtgattca tatgatccat aaacgtctgc atcgcggggt
     4921 gaccagtatc aacgacgcag aagggactta caagcatgaa caaaaagcag ttttgattac
     4981 cattttgaca tgtgaagaat acccagaatt caaatggctg atgttaaaaa cagacccaca
     5041 agcctttgtt tcagtggctg agaatgttag aattatcggt cgttttgtgg aagatgatta
     5101 agaaaaacac aggaggtgtc atgtcaattg accaacgttt acagttaaag aaattttgct
     5161 ttattatttt aggagctgct atctacgcct ttgcttttgt ttatttttac atggctaatc
     5221 gcattgcagc caatggttta gctggtctca ccctagttgg taaggcactt tttgagattg
     5281 atccttcttt gataggctat ttgattaatt tgcctttggt cttgctggga gctagatgtt
     5341 ttgggaaaag agccatggtt tacacagtaa cgggtgtttt atcactttac ttttttgtgt
     5401 ggctgtttca acgctttcct ttggtcgttg acttggacca tgacaattta gtcgtttcct
     5461 tgatggctgg aattattggt ggtctaggtg gcggttttgt gtttcgcaat ggtggaacca
     5521 tcggtggagc agatattatt gctaagttat tagaagacaa gttaggacta cagctgaatc
     5581 aagctctttt aggcattgat ctttttgtca tgattgtctc tttgacttat atttccctgc
     5641 cgcaaatgat gtatgctctg attgctagct ttatgtatgg tcaaattgtt cgcttggtac
     5701 aacaaggtgg ttattcagcc cgtggcattt ttatcgtttc agaacaagcg gagaaaattg
     5761 cccggttcat catggaagaa ttgggaaggg gagttactta tctaacaggt gaaggagcct
     5821 actctggtcg ccgtaaaaaa gtgatttatg tggctttggg gccaagagat attagggaat
     5881 taaaagcttt tttaactcag gttgatccta atgcctttat cacttatttt gatgttaatg
     5941 aagttaatag cccagaattt ttaactatta aaagcaaata ccataaaaag agaaaataaa
     6001 tgagcattag agttgcctgc gaagaatagt gaggataaag gatggcacat cacgataaat
     6061 tgactaaact actaaaactg tttttgattg ccctaggtgt agccatttat accttcggtt
     6121 ttgttaattt taacatggct aatgctttgg ctgaaggtgg tgtggcagga ataaccttga
     6181 ttttacacgc gcattttggg attaatcctg cttactcttc tctcttattt aatcttccac
     6241 tttttatttt aggggcaaag atttttggga agcgttcttt ggctctaacc atctatggaa
     6301 cagttctgat gtccgctttt atctggatgt ggcaaaaagt tcctatcgaa cttggcttgg
     6361 aaaatgacat gatgttggtg gctgtagtgg caggtctctt ttcagggatt gggagtggca
     6421 ttgtttttcg ctatggtgca accacaggtg gaacggatat tattggtcgc attgcagaag
     6481 aaaaatttgg agctaagcta ggtcaaactt tgcttttggt ggatgctctt gtattgacag
     6541 cttcgttgac ttatgtggat ttaaaacaca tgctttatac tttggtggca agctttgttt
     6601 ttagccaaat gattagtgtg gttcaaaatg gtggttacac tatccgtgga atgattatta
     6661 ttaccaagca ttcagaggct gccgctcaag ctatcctaac cgaaatcaat cgtggagtga
     6721 cttacttgaa aggtcaagga gcttactctg gtaatgatta caatatcatg tatgtgacct
     6781 tgaacccaac tgaagttcgg gaagtcaaac gtattttagc tggtttggat ccagatgcct
     6841 ttatctccat tattgatgtg gacgaagtta ttagctctga ttttaaaatt cgccgaagaa
     6901 attatgataa ataaaaatac aggtcaaaca gcctgtattt ttattttggt cccccactta
     6961 agaaactacc aagcataata gctgatatag cagttccctt gtaatggcta gcttttgttt
     7021 ttaccaagcg actaagatca aatatgcttt taggagcatc aaattgatgt gttaagatgt
     7081 attgattgat aagtgagtca atttttgtag ctaggagtga gctcatcccc tctttttcaa
     7141 gttgactggc gagctcgaag atcgcttgac gaaaataatc atcggcttgt attacttgtt
     7201 cgctataaac tttactaaga gcatctaata tttctttttc tgatggcatg gcattctcct
     7261 gtaacatatt gtgctattat tataatataa aaaactaaga ctgtcataaa tgtgacagcc
     7321 ttagtaatgt tattacttac atttcatttg gtgcgtcaac accgagtaag cgaagcgctt
     7381 ctttaagaac agtagcagtt gcatagcaga gagctaagcg gttatcacgt tctgagttgt
     7441 cgtcaagaat gcgagtgtga gcataatatt tgttaaagct ttgggctaag ttaatagcaa
     7501 atttagccat aatagaaggc tcaaagttat ctgatgttcg cttgataatg cgtgggaaat
     7561 cttggataag tttgataatt tcccagcttt cagcatcagc taaactgtaa gtggtggttg
     7621 cagatggcgt aaagtcagct ttacgtagaa tagattggat acgagcatga gcatattgaa
     7681 cgtaaggtcc agtttctcct tcaaaggaaa ccatggcttc aaggtcaaag tcatacccat
     7741 tcatacgatc tgttttgaga tcgtaaaact tgatggcacc gacaccaaca gagtgtgcaa
     7801 cggcttcttt gtcagcaagg tttggatttt tcgcttcaat ttgagaagct gcacggttaa
     7861 tggcttcagc aaccgtaggt tcaagaagaa taacattacc tttacgtgtt gataatttag
     7921 caccgccttt tgtgacaagt ccaaaagcaa cgtgtgtcat atcatctgac caatcgtaac
     7981 ccatttcttt tagcactgct tttaattgtt tgaagtgagc agcttgctca ttaccaacaa
     8041 cataaacgga tttagcaaag tcataagtgc gtttacggta aagtgcggca gctaggtcac
     8101 gggtaatgta aagagtagcg ccatctgatt ttttgatcaa ggcagggtgt tcaatgccat
     8161 atttttcaag attaacaact tgggcacctt tagattctac aagaaggttt ttagcttcta
     8221 ataagtcaag aacctcatcc attttgtcat tatagaaggc ctcaccgttg taactatcga
     8281 aggtgacatg taattggtca taaagtcggt tgaactcaag taaactttca tcacggaacc
     8341 attgccataa ttcagtagct tctttgtcgc catcttccaa tttacggaac cattcacgag
     8401 cttcttcatc aacggtggga tcagtttcag cttcagcatt gatacgaacg taaagtttta
     8461 agagttcatc aataggatgt gcctgaacag cagcttcatc tccccatttt ttgtaggcaa
     8521 caatgagcat accaaattgt ttaccccagt caccaaggtg attgattttt acaggtttat
     8581 agcccatttt ggcaaaaatg tgagcgagac tatcaccaat aactgttgag cggaggtgac
     8641 cgattgaaaa tggcttagcg atgtttgggc ttgacatgtc aatagcaaca tttcgccctt
     8701 gaccttcgtc ttgctgagca tagtctgaac ctgctgtaat cacttgttca aggacctgtg
     8761 aggaaatctt agttttatca aggaaaaagt tgatgtaagg gccgacagct acgacttttt
     8821 caaattggct ctcatcaatc tgttcagcta actcgctagc aatcatttga ggggctttgc
     8881 gaaggacttt tgctaaacta aaggctggaa aggcaaggtc tcccatgtca gaattttttg
     8941 gggtttcaag caagttaaaa atagcatctt gttctaattc tggaacaact ttagctattt
     9001 cacttgcgat tagagtttta gtatccatac agcgtctcct aaaaattgtt tgtcacccta
     9061 ttctaacata tttctttaaa ataaacgagg agaggattga taaattatac tgaaactttc
     9121 aaggaaatga attgcttgag gttgttgttt ttctgaaaat gttataaaaa gagaaatgag
     9181 gtttcttttc aacagttttc aagtgaaatt ctagttgaat ttgctaaaaa attgctgaaa
     9241 atagctgggc gaagatgaaa taatacactt tttttgttat aatttgagag gataattata
     9301 caattggggt gatatatcat gaataaaatg gagcgtcaac agcagattaa gcgaatcatt
     9361 caagcagagc atattggcac acaagaagat attaaaaatc accttcaaaa agaaggcatt
     9421 gtggtcaccc aagcaactct atctcgggac ttaagagaaa ttggcctttt gaaattgaga
     9481 gatgaacaag gcaaactcta ttatagtttg tctgaacccg ttgcaacacc atttagtcct
     9541 gaggtgcgtt tttatgtttt gaaagttgat cgagcaggtt ttatgctggt tctccatacc
     9601 aatttaggag aggctgatgt tctagctaat ttgattgaca atgatgccat tgaagatatt
     9661 ctaggaacca ttgctggtgc ggataccttg cttgtgattt gcagagacga agagattgct
     9721 aaacgctttg aaaaagattt ggctgcaggc ctatgactag ctattaagag tctttgacac
     9781 aattgattct tgctatccat gaattctagt atcgttgctt ataaagaagt gcagcaatag
     9841 ctgtctaagc tccgtgattt taaaaagtaa gcttatcaga tgaaacgcta tcaacaagat
     9901 gccctgcttt tcaaaaaaaa tagataaaga aaaggctgcg acagtatctg caagcagggc
     9961 aaaagaacta gaagataggc tcagtcatca gccattaatt gatgattatc gagaaaagat
    10021 gcaagatgca agtgatgtga ttcagtatat caccaaacgt atagaagatc agttaaacaa
    10081 ggagttaaca aatggcaaaa actaacattt ctcctggaat gcaacagtat ctggacatca
    10141 aaaaagatta tccagatgct tttttgcttt ttaggatggg tgacttttat gaattatttt
    10201 acgaggacgc tgtcaaagca gcacaactct tagaaattgg tttgaccagt cgcaacaaga
    10261 atgcggaaaa tccaattcct atggcaggcg tgccacatca ttctgcccaa caatacattg
    10321 atgtgttaat tgagttgggt tacaaggttg ctgtcgcaga acaaatggaa gacccaaagc
    10381 aagctgttgg ggtggtgaag cgtgaggtcg ttcaagtcat aactcctgga acggttgtgg
    10441 attcagctaa gccagatagc gccaataact ttttggtagc tgttgacttt gatggttgcc
    10501 gttatggatt ggcttatatg gatgtgtcca caggtgaatt ttgcgtgaca gatttggcgg
    10561 actttacgag tgttcgtagc gaaatccaaa acctcaaggc aaaagaagtc ttactaggtt
    10621 ttgatttatc tgaagaagaa cagacgattt tggtcaagca gatgaatttg ctgctttctt
    10681 atgaagaaac ggtctatgaa gataaatctt taattgacgg ccaattgaca acggtagaac
    10741 tgacagcggc aggaaaactc ttgcaatacg ttcacaaaac acaaatgcga gaactcagcc
    10801 acttgcaagc attggttcac tatgagatca aggattattt gcagatgtcg tatgccacta
    10861 agtcaagttt agatttggta gaaaatgcta ggactaataa aaaacatgga agtctctatt
    10921 ggctgttaga tgaaaccaag acagctatgg ggatgaggct tttgcgctca tggattgatc
    10981 gacctttggt ttctaaagaa gctattttag agcgtcaaga aattattcaa gtttttctga
    11041 atgcttttat tgagcgaacc gatttaagca atagtttaaa aggtgtttac gacatcgaac
    11101 gcttatctag tcgcgtgtct tttggtaagg caaatccgaa agatttactt caattggggc
    11161 ataccttagc ccaagtgcct tatatcaaag ctatcttaga gtcttttgac agtccttgtg
    11221 ttgacaaact tgtcaatgat attgacagtt tgcctgagtt ggaatacttg attagaacag
    11281 ccattgatcc agatgcacca gcaactatta gtgaaggcag tattattcgt actggttttg
    11341 atgagcgctt ggaccattat cgtaaagtga tgcgagaagg aacaggttgg attgcggata
    11401 ttgaggctaa agagcgtcaa gcaagcggca ttaataacct aaaaattgat tacaataaaa
    11461 aagatggtta ttattttcac gttacgaatt caaatcttag cttagttccc gagcattttt
    11521 tcagaaaggc aactttaaaa aattctgagc gttatggaac agcagaattg gctaagattg
    11581 aaggtcagat gttagaggct agggaagagt catctagttt agaatacgat atttttatgt
    11641 gtattcgagc tcaagttgaa acctatatta atcgtttaca gaaactggct aaaattttgg
    11701 caacggtaga tgttttgcaa agtttagcag tcgttgctga aaccaatcat tatatccggc
    11761 cgcagttcaa tgataatcat gtgattacaa ttcaagaagg tcgtcacgcg gttgttgaaa
    11821 aggttatggg agtgcaggaa tacattccca atagtatctc ttttgaccaa cagaccagta
    11881 ttcagctgat tacaggtcca aatatgagtg gtaagtcgac ttatatgaga cagctggcct
    11941 taacggttat catggcccag atgggttcat ttgtggctgc tgaccatgtt gatttacctt
    12001 tatttgatgc gatttttacg cgtattgggg ctgctgatga tttgatttct gggcaatcaa
    12061 cctttatggt ggagatgatg gaagcaaacc aagcaatcaa acgcgcaagt gacaactctc
    12121 ttattctatt tgatgaactg ggacgaggta cggcaactta tgatggtatg gctttagccc
    12181 aggcaattat tgaatatatc catgatagag ttggtgctaa gaccatattt gcaacgcatt
    12241 atcatgaatt gacagacttg tcaactaagt tgacaagtct agtcaatgtt catgtagcaa
    12301 cgcttgaaaa agatggcgat gttaccttcc ttcataagat tgctgaggga ccggcggata
    12361 aatcttacgg tattcatgtg gcaaaaatag caggactgcc aaaatcccta ttaaagagag
    12421 cagacgaagt tctgacccgt ttagaaacac agtcacgatc tactgagata atatcagtcc
    12481 cttcacaagt tgagtcaagc agcgctgtta gacaggggca attatccctt tttggtgatg
    12541 aagagaaagc tcatgagatt aggcaagcac tggaagctat tgatgtcatg aacatgaccc
    12601 cgcttcaagc aatgacaacc ctttacgaat tgaaaaagtt gttatagtct ttcagctgaa
    12661 aatgaaaaag atgctttcta tattgaaggc atctttttgt tctgtcaaaa acggtccgag
    12721 gccttcggct atttattaag tgtgttataa tagtccataa gaatgcgagg aaattatgac
    12781 aaacattatt ggacttggat tggcagtctt tacagggtat tgacagtcct taagcattgt
    12841 tatgactgtg tttttgaaaa aatataggtt ttaagtttcc ttagacttcc ctcaaaagtc
    12901 cacaaaaagg tgaacaaaaa aagaccttta caggtccttt acacaatgag ttcagcaggc
    12961 aagaactagc gtggtttaaa tactacgctt tttagtttgc cctatggctt attatagcac
    13021 gattagtgtc attgaggaaa aaactaccct ctcaaccagt ctttaaagtt ccaccattgg
    13081 tcttggatgc tatcgctcaa gcgttgatgc caagtttttt tggattcttc ttcgatagaa
    13141 cgcttgaata acacctccgc atattgttgc tcttcggcat ctctctcttt ttgaagacgt
    13201 tcattatgtg tttttccatc ctcaaataca agttgcccaa agtatagatt cccgtcatcc
    13261 gcaaaagata aggtaacttt gtctccttca gcaagtggtt tttccaattg aactgtgtag
    13321 ttgcctcctc catcagcacg attgccactt actgtccatt tccttccttc gcagtttgga
    13381 ttatcttgtt tctccaaaat agagcaagga atttcataag ttttgttttc ggttttttta
    13441 attttattac cattacttga agttacaaaa ccataaacat acccataggg atctagatta
    13501 acattaatgg ttgtatctcc tgttttaaat ggtgctacgg ttactccagg taagtgcgat
    13561 tgccctatag ttgtaaacaa atcgccactt tcattagctc tgacacttat agcacatatt
    13621 gatattaaca aagttaacat cataactaat ttctttttca ctatattctc cttaaaatta
    13681 aatatcgaac tccacaatac cagttaatca gtcaaaaaat ccattctact ttttgatatt
    13741 ttatgtctaa tttgacccgc taagtcagat ttgcaaacga taccaagtga ttttcttcta
    13801 atcattattt tttagatgga tatgttaatt gtattagaga cgtattaatc ttaaaccatt
    13861 gtcataagcg aacattattt tttagcgccc cgtctctgta aaattccaca aactcaagca
    13921 aggctctttt cttagattca taataccagc tttgacttct gttaagttct tccataatgt
    13981 cttgttgagt tttttcctca ctgatcaagt aacactcaat tagtatttgt ctatattcta
    14041 ctttagacag ttgattaatg gcatacctaa tagcgtctag ttcctctagg gcgcattctc
    14101 ggcttatttc aaggtgtttt ctgcgcgtgg gatggtactc tatatcaaac tggtaaagct
    14161 cggtataagt taaatcaagg ctattagcga tacgttgcca tctatgaaat tctttcagct
    14221 tacgaatagc gttcttcttg ctcatctaac acctctaaag cttctctatg tagtttgaac
    14281 acaatgtttc ttgagtaacc tagtttatcg agtatctcgt cccatgataa gtcatccacg
    14341 tatctagcct taataacagc tatctgtctc tcatcttgga gcgtggcgat catggctagt
    14401 ctcttatcac gttctttagc taaatataag agttgtttag ctgtaccttt ttcgatatgt
    14461 cttattgctt caggattatg aaaagcattt atcagctcga tgtctttatc tcgttgttcc
    14521 tcaaataagg ttattaaagc gaaaagtggt ttcaattctt taagttgttc tttagcgctc
    14581 atggtctttt ctcctggtta tggtataatt tatttaagct taaatttaac caagggggcg
    14641 ttcgtatgga cgtctttttg ttttgttcgt tttgtaaact agatattttt tgtagtactt
    14701 cgcttttcgc ttctaaaatc gtttctaagc actttttaga gtttatagta taaatcatca
    14761 acttagtagc tagaagcgct aaaacaatgt tttattttag tcttagatgg ttattctttg
    14821 tggcagggtt atgggctaaa atgagatgtc aaatcctttc ctatcaagct ttttaatctt
    14881 cgtcaaaaat ttcaaaaagg gaattttttg cacggaaaag ggcgcgttct taagtttccg
    14941 aacaatatag tcccgtttaa aaatgaaggg ggtagtttcc gaatattata gccagtacct
    15001 gtcaccctta tccatatagt aatgaaatct tctccagtga taaaggtatt tggttaatct
    15061 catgtacttt ggacgtttag gaaagtcatc acgactataa tatccatgaa tgtgtcttgc
    15121 ttctggattt actttcaaac actctttaaa ggctaattgc cagtagtagc aacagtctgt
    15181 cttgcttcga ttgagcgtgg cttgatgaac cttctgacaa gaaccacaag caaaggcatg
    15241 agaagcctta aataatttcc gacaacgtct ctcacagtca ggacacacga aaaagtaacg
    15301 tttaccacca taagttcctg gaatcgtttc aagcaatagg ccttgaccat tgtagtgaat
    15361 cactagccca tctaggtcta tgcggatagc ttggtcacct attgatcctg taactcttgt
    15421 cttcccctgt gtcttcattg gtttaatgat attctcaata gatagttcta acattatttc
    15481 tccttaaaac tcaaacaacc caaaactatt gataaaacga caaaaagagg aaaaaccctc
    15541 tgaatgtcta tttaacgagt aactgacctt caacaaccat atcatacaaa tggttaaagg
    15601 cttgactgat agactcaagg atggccccta agtcttctgg cgtcatctct ttataattca
    15661 tagagaggtg ttcagccagt tggttgtgat cggagatgaa ggctataagt gtgtctcgat
    15721 tattaacttt cccttgagta cttttagata aagaaaccac gcgctgatga tcaagttctt
    15781 catccgtcat atcctcaggt tggttgtaat agtccttgaa gctgtcacag atacgcttga
    15841 agactttgtt tagttttctg tcttcaacat attttaagac taactgatta gcatgaccac
    15901 cttggtcatc attgtgataa gttgcgtcaa tcactggttg ctcataagta ccagtcatat
    15961 agcctaaaat agcatgacaa gccacttgtg cggtgtcaaa gtctttaaac gtgtagtgga
    16021 atgtgaatgt ctttggtgtg tctgaaaatg ttctcatgct atttctcctt tgtgattgct
    16081 ataatgtctg ataaattgat aatggcagaa ggagatgcta cccagtttgg ttgttctcca
    16141 gataaaagat acttgaccaa ctcattataa agggtgcggt ctccttgtat ggtgatggtg
    16201 ttaccacctc gtgtgtgtaa ttttagtttc atatcagtta cctgtacaag accagtagtc
    16261 ctgttcttga atccatgtct tcataattgc cataagtggc ttcttggaat ttaatgtcta
    16321 tcacagatac cgatagggta aagagattga cccgatgttc aaaatcatct agtgattcat
    16381 tgtgtttttg ataaaatagt ttgattttca tgttttagct cctttctcaa caggtatcgc
    16441 aaagccacag tcaaagaccc attggtaacc acgtcgtgtt agttctggtc tggtaaaatc
    16501 acaatcaccc ggtctagact ttatgttgaa attaattttg gtgataaagt cataaccgca
    16561 ttgaccatta ggaagttcca atcggtatag ctggtcgttt ggcaaatcat cataattgta
    16621 acgcattttc tttacgccat gatttgtata gttaggattc atatgtccct cttttctctc
    16681 taaaatgtgt gctttttgtt tttttaaaaa tcaaatagca cagcaaaaag cacagggata
    16741 aagccagtgg tatcaagggg gttgggcact ttgtgctttt agtagcatta atttgagcaa
    16801 aagactttct ttttttgcac gcgcactatt agttataaat attattatct ataatattaa
    16861 atactactaa tactacaata gagtataaag cctataatac caaggtttta gcttgtatca
    16921 ttacgtgtag tttttatgta gcattagtgt agtttttaaa ctcgttcata gtaaggtacg
    16981 ggagttccac ctttaaatag tctttttctt gcgggattct ctcctttttt ccagccgtca
    17041 tcattatcta agtaatcacg tattttttga gaaataaggg ccttaccacc ttgcgttggt
    17101 ttttggttaa atcctagata agcgatatgg ttaggacttg tcacttggag taagcaatct
    17161 gtctgtgcgg aagggtagtc actatagctt tgagcattat ctaacggctc tcctagctgt
    17221 ttgaggatgt attgccgttg ttcatactgt gataagctat cccaaccttc aacaatttga
    17281 aactcattca gtaattgatc gataatttct ttgtcgacgt cttcaacctt ataatcttct
    17341 tgaatgtctg ctaactgatt cattaactct ttagatggcg ttagtggttc ataattgtta
    17401 aaccatactt tggcttcagc gagtacctgt aagaaataat cttcttccac ctccatagga
    17461 tgttttttca cgtcattgat accacattca atagggaaaa agcgtctttc tgttccacta
    17521 tccttaagaa aagatttttt attagctgtt ccgataaaga cacagtgcct tggatgagga
    17581 gtggctttac gttcataagg ttcacgataa gtatcactat ctgaggaaat gaagctttta
    17641 actgtttcaa tttctgcctt tgacatgcct tttagctccc ctagctcaat aatggcattg
    17701 gcttgtatct tctgataatc actatcattt ttaccaaact tgatttctga atcagtgtgg
    17761 tagctaggga gtagtcgctt agtaacggtg cttttcccag ttccttgtct tttatcaatg
    17821 agaataggaa cgacttcaaa ctttacttta cggagataaa ttctagccat gagacctgtt
    17881 agccatactt tggcaatttc tctattatag gaattatcag cacagcctaa tagatcaata
    17941 aagtagcgtt ctcctctagc tttaccatcc catttttgac tttcaatacg ctgtttaatg
    18001 gggtgataag tgttcttttt agctaaagcg gtaatagcta cctctatatg ttctttacga
    18061 ggggtaaacc gatatttttc atcaatgaat gcaatacaaa ggctggtctg ctcgttcgtc
    18121 catagccctt tttctttaga ccaaggaact gctttagtga tttcaatagt tttttcaaat
    18181 tcgttgtatt taatacctgt atagatattt tcgtaaaatt caaaaacctt accgacattg
    18241 taggggctac taatgacata ttctttgtct cctctacctt ttcgtgtcct gaaagcaggg
    18301 gcaaaagcag gttgagtggc ttgcgatagt ttattttgat agtctttcaa ttcttctttg
    18361 tctatggctt gattcctctc tttcttaatt ctttatcaag tatgcttcta aaggttgtat
    18421 ctatctcatc aatgggtagt ggcttagttg tcacgctgtt agctatttgt accagctcat
    18481 aagccgtctc taaatcacaa tccacccatt tattaaatag caagccaaca aacttagtta
    18541 aggccacgtt gcgcccgcct tcgtctccaa aaccattaaa caaggtatct atgaccctca
    18601 tggtaataga acgctgactt ctagggcgtg gcgtgtaagt agtaacaact tgtctgtttg
    18661 gcgtgctacc atttttagga acaggataat caagaccatg gttcacatag cgctgatagt
    18721 cctctgggtc gcctgttgta acgggtaagc cttgtaattg cgaccaggta agactagcta
    18781 aatcaaacgg cagtccaatc ttatcggcta tctccttgac cacttgttta taagttgctt
    18841 cagtcatcac gtcactaggc ttcatgacaa ggcgataacg gggcttctcg ggggtgtgtt
    18901 taatcgttgg ataaataata taactatact cccaaagcgt ctgagaaacg attttaggta
    18961 ggttgacgcc tgtttctatc tcgtcatagt caagaaaaat caaatcgcga taaactaaac
    19021 tagcattatt gcgcttatag ctaccgtttt tctctgctgt gaccttgcca cttaggcagt
    19081 agggggcttg tgttcgcttg tattcttcaa tatcaatatc ctcaggcggt ttcaaaggtc
    19141 taaactgagc aacatagtca aatggttcta aaggtccttt gtaggggtac aaataagagc
    19201 taaagcctct tgcttcataa atagccatct acacatttac ccccaaaaag ataagaatat
    19261 cactgacctt gtaataatgt ttcctggtgt cttctagtgg tggttggtat cgtcttaacc
    19321 cagcattttc ccaccgtttt agggttttac ctttgatatt taattcctct ttgacttgtt
    19381 cggccgtgat caaccctaaa actcttggtt taggtttctg gtaggcttcc aaaaagtgat
    19441 taaaagcggt caggttttgt tctaagagtt tggcttcata atcttgacta aatacgttca
    19501 tgcctaacct cctttgagta attccttata actggttaaa tcggcattca ataacacact
    19561 taggcgttcc tgttcctttt gtacttgatt ataaaaggct ttagcaccat ctagtaattc
    19621 ttctttgtta gctgggataa agtacccacg attgaatccg tgtctaatgc cgataatagg
    19681 gacgttatag cgcgtgatta agctactgat gatactttgg acggagcgtt cttcaagttt
    19741 cagtattaag ctaatctctg cccctgtaat ggggttgtct gctccaacct tgatcagatt
    19801 aaggacacgt ctataattct ctggtagtgt cattcagttc ctccctaatt gtaataatgg
    19861 ttctgtgctt gaatataagc cccatagttt gcgttctgac gtggtttagg tgcgtgggta
    19921 tcttctggta agtcaatctc tattaacggc ttagaacggc taagaagaag ccctaagaga
    19981 cctaaaacaa agaatagaat aagcgtctgt gttggtgtga ggttaagttc ttgcatcatg
    20041 ccgatacctc acttaaataa gtttctagtt cccctgagtc tttctctgaa caaggtaaac
    20101 cgttaacggc tctaaagaca atctctgtgg ttcgttgata gtctaaagcg tcccatgctt
    20161 cttcaaagct ggtggcactt tttctgaatt taatgacgta ctctgtcata acgttagcaa
    20221 taattaccca agcaatatgt tggttatata gtcgagtgaa atagacttca gctttatctt
    20281 tgctgagttg gcgatttttg aacatttcta gctgttcagg agtgtatcta tcttttgaaa
    20341 aaggatttgt ttctactcta tatttcatta tgtcttttct cgcttaatta ttattttctg
    20401 tgtagtgttt ttattgattg cttgtttctt atactagatt catgctagtt ttaaggggta
    20461 gctccctgat tagttcatgt tagtgtataa ttctgcgaat aactcgctag ggatacgctc
    20521 tagcgctttt tgttgtaagt ggatggcttt aattctatct tgtgttttgg ttttaatgtc
    20581 ttctataatt tcagatgttg agaccacttg ttcatagtaa atgttagctt tataaatgag
    20641 tccttgttct tttaattcct tattagccat tttttcaagc gtaacttcat gtaatacttc
    20701 aacattacga taatgaccgt ttttgaggtc gaattttagc cattttttac gcttccattt
    20761 atatagggtg cttctgcaaa cttctgaatt cccaaaacca agaaaagaag cgatttctgt
    20821 taatgttttt ccttcaattt cagatagttt ataagcgacg tttctaaata acactctcgg
    20881 atttcttttt ttcttaatca tattgtcttg atttttgagc acacaaaaag cgcactccct
    20941 ttctatgaat tttaggttca caaaatagag tacgcatgat atactattta cgtacctact
    21001 ttgtgggtgc tgggagttcc taacgtgtac ggtcgccaaa ctataccacg ttaggaactt
    21061 ttttattttt cagactcata agagtttaca gctttaacga ttaaatccgc tttagataat
    21121 ccatttttat ccgctgtttc ttgtattgta ttatactcgt cagcggtcaa acgaacttcc
    21181 agccgtttat cacgtttagc tgtacctttt acaggtctgc ccatttttgg actcatagga
    21241 gttcctcctt tcatttaagc ccgtgcttat atcatatgat aagcacgtac ataagtcaac
    21301 ccctaaatta aaaaaacatg cgtattctat tttcaatgtg caaatggttc acaaaataga
    21361 gtacgcatga tatactattt acgtacctac tttgtgggtg ctggctctat cacgttgacg
    21421 gtttggcgat tgtaaacgtg gtagagtact tttttatttt gtaagatttt caaaggcttt
    21481 tctcaaagtc tcgggtttag tcaagttatt ctctttagcg tacttattta gtttgctatc
    21541 taactcttca ctaaccctta ccgttaattt aacagtattt gagttttctc cctttggtcg
    21601 tcctactcgt tttttggtgt cggtcattga gtttatcact cctttcatgt cgacaaaaac
    21661 tattatagtt tatgtcgtca ataattgtca acccctaaac caaacttttt ttgcgtactc
    21721 tattttattt tcaatgatca gtatgataca atggaagtat caaatattta ctaaaaccct
    21781 tttaataata gcttgcctgc tttattaatt gagtttagtt atactagttg aaggcttgga
    21841 agtttggtcg ctgtcaaagc cttttttgtt gttttcacgc gcattgtagc gtgtttttta
    21901 atgccattgt cttaatatct tgataggtaa aattaagatt gataagagcg attgccatat
    21961 cttctaaagc agtgtactgt gctagttcaa ttgagtttaa gcagtcaata tcagaataac
    22021 cacctctggt tacttttaat tgcttgctat tcttaccagt aacagcttta agcaatagat
    22081 tgtacacagt cggataagcc attttaggcg cgtgttccca tgtcttgata gcttcattaa
    22141 gtgtttttcg tttaggagct tcaagagaac gctgtaacct aatttgagtg agttcttctc
    22201 gcatttcaaa gaatgccttg actaggttct ttttaaactc tctgactggt tcggtatttc
    22261 ctagataggt aactaataaa gtcgcctgct gttcgtttaa gtgataaatt cttctaggtc
    22321 gtccgcctag tgaacctttt tcaggtttat ggatttcaaa tgacaaaacc ccaaacgctt
    22381 c
//
LOCUS       DATXIG010000021        22342 bp    DNA     linear   BCT 28-DEC-2023
DEFINITION  TPA_asm: Streptococcus pyogenes strain MGAS11113
            SAMN08555799-rid19682583.denovo.021, whole genome shotgun sequence.
ACCESSION   DATXIG010000021 DATXIG010000000
VERSION     DATXIG010000021.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN08555799
            Sequence Read Archive: SRR6740495
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pyogenes
  ORGANISM  Streptococcus pyogenes
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 22342)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 22342)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-OCT-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-OCT-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001918058.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001918058.2
            Genome Coverage       :: 368x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/12/2023 11:23:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 1,806
            CDSs (total)                      :: 1,769
            Genes (coding)                    :: 1,722
            CDSs (with protein)               :: 1,722
            Genes (RNA)                       :: 37
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1, 1 (5S, 23S)
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 47
            CDSs (without protein)            :: 47
            Pseudo Genes (ambiguous residues) :: 0 of 47
            Pseudo Genes (frameshifted)       :: 30 of 47
            Pseudo Genes (incomplete)         :: 20 of 47
            Pseudo Genes (internal stop)      :: 9 of 47
            Pseudo Genes (multiple problems)  :: 11 of 47
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..22342
                     /organism="Streptococcus pyogenes"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN08555799-rid19682583.denovo.021"
                     /strain="MGAS11113"
                     /serotype="Emm28"
                     /isolation_source="normally sterile site"
                     /host="Homo sapiens"
                     /db_xref="taxon:1314"
                     /country="Finland"
                     /collection_date="2002"
                     /collected_by="National Public Health Laboratory"
     gene            <1..1146
                     /locus_tag="VC122_000899"
     CDS             <1..1146
                     /locus_tag="VC122_000899"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003061491.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent rRNA methyltransferase"
                     /protein_id="HEP5640835.1"
                     /translation="DSFVEKKLTAGVQLLDEKDFSNIKEKNQLVQLVTKSNRPIGTAY
                     ISKQNKGIGWYLGPEKIDLSISYFVSLFSVAKAKRQDFAQSDETNAYRLFNQEGDGFG
                     GVTIDLYKDFAVFSWYNAFVYDKKEMIMEAFQQVFPEVKGAYEKCRFKGPDTETAHLY
                     GELAQETFSILENGIAYQVFLNEGLMTGVFLDQHDVRRALVDGLAMGKSLLNLFSYTA
                     AFSVAAAMGGAIETTSVDLAKRSRELSLAHFEHNQLNLASHHFVVMDVFEYFKYAKRK
                     KLIFDVIVIDPPSFARNKKQTFSVSRDYHKLITEALDILSPKGTIIASTNAANMTVSQ
                     FKKQIIKGFGSRRPESMTLQQLPSDFTINKADERSNYLKVFTIKVRE"
     gene            1143..1829
                     /locus_tag="VC122_000900"
     CDS             1143..1829
                     /locus_tag="VC122_000900"
                     /EC_number="4.2.1.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002985140.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I 3-dehydroquinate dehydratase"
                     /protein_id="HEP5640836.1"
                     /translation="MRIVAPVMPRHFDEAQAIDISKYEDVNLIEWRADFLPKDEIVAV
                     APAIFEKFAGKEIIFTLRTVQEGGNITLSSQEYVDIIKEINAIYNPDYIDFEYFTHKS
                     VFQEMLDFPNLILSYHNFEETPENLMEAFSEMTKLAPRVVKIAVMPQSEQDVLDLMNY
                     TRGFKTLNPEQEFATISMGKLGRLSRFAGDVIGSSWTYVSLDHVSGPGQVTLNDMKRI
                     IEVLEMDISN"
     gene            1923..3089
                     /gene="aroC"
                     /locus_tag="VC122_000901"
     CDS             1923..3089
                     /gene="aroC"
                     /locus_tag="VC122_000901"
                     /EC_number="4.2.3.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003048042.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chorismate synthase"
                     /protein_id="HEP5640837.1"
                     /translation="MRYLTAGESHGPSLTAIIEGIPAGLTLHPADIDHELQRRQGGYG
                     RGARMSIETDRVQISSGVRHGKTTGAPITLTVINKDHQKWLDVMAVGDIEETLKLKRR
                     VKHPRPGHADLVGGIKYHFNDLRDALERSSARETTMRVAVGAVAKRILAELGIDMLHH
                     ILIFGGITITIPSKLSFRELQERALHSELSIVNPKQEEEIKTYIDKIKKEGDTIGGII
                     ETIVQGVPAGLGSYVQWDKKLDAKLAQAVLSINAFKGVEFGVGFDMGFQKGSQVMDEI
                     TWTPTQGYGRQTNHLGGFEGGMTTGQPLVVKGVMKPIPTLYKPLMSVDIDSHEPYKAT
                     VERSDPTALPAAGVIMENVVATVLAKEILETFSSTTMSELQKAFSDYRAYVKQF"
     gene            3150..3491
                     /locus_tag="VC122_000902"
     CDS             3150..3491
                     /locus_tag="VC122_000902"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002985134.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YlbF/YmcA family competence regulator"
                     /protein_id="HEP5640838.1"
                     /translation="MSQEIYDYANQLERAVRALPEYQKVLEVKEAIQADASASELFDE
                     FVAMQEKIQGMMQSGQMPTAEEQTSIQELSQKIEANDQLKAYFEAQQALSVYMSDIER
                     IVFAPLKDLVK"
     gene            3713..5065
                     /gene="gorA"
                     /locus_tag="VC122_000903"
     CDS             3713..5065
                     /gene="gorA"
                     /locus_tag="VC122_000903"
                     /EC_number="1.8.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002985132.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione-disulfide reductase"
                     /protein_id="HEP5640839.1"
                     /translation="MVIPYDYIVIGGGSAGIASANRAAMHGAKVLLAEGKEIGGTCVN
                     LGCVPKKVMWYGAQVADILGTYAKDYGFDFKDKAFDFKQLKANRQAYIDRIHASYERG
                     FEQNGVDRIYDYAVFKDAHTVEIAGQLYTAPHILIATGGHPVFPDIEGAQYGISSDGF
                     FALDEVPKRTAVVGAGYIAVELAGVLHALGSKTDLFIRHDRPLRSFDKAIVDVLVEEM
                     AVGGPCLHPHSEVAKVVKNTDESLTLYLKDGQEVEVDQLIWAIGRKPNLEGFGLDKTG
                     VTLNDKGYIETDAYENTSVKGIYAVGDVNGKLALTPVAVAAGRRLSERLFNGKTDEKL
                     DYQNVATVIFSHPVIGSVGLSEEAAVKQYGQEAVKTYQSRFTSMFTAITNHRQPCLMK
                     LVTVGDTEKIVGLHGIGYGVDEMIQGFAVAIKMGATKADFDNTVAIHPTGSEEFVTMR
                     "
     gene            complement(5153..6421)
                     /locus_tag="VC122_000904"
     CDS             complement(5153..6421)
                     /locus_tag="VC122_000904"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002990257.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional folylpolyglutamate
                     synthase/dihydrofolate synthase"
                     /protein_id="HEP5640840.1"
                     /translation="MTTYDSISWIHTFKANGRKTDLARMAWLLEALGRPQDKFPAIHV
                     VGTNGKGSVTAYLQHILSTSGYQVGTFTSPFIISFHDRICLNGQPISDLELAQCVSVI
                     RPILTKMSLETNWDRPTEFELVTLIMFCYFANLKPVDIAIIEAGIGGKTDATNVFHAL
                     AVVCPSISFDHQERLGYSLAEIAHQKAEVIKAKEPVIIGQLEQEASQVFQQVTQKAGS
                     RLYQLGKDFLLNPSEKTFSFQHDNLNLTKLRLKLLGQHQKANACLAIMTAQLLRKSFP
                     KISPKTIQKGIEATTWPGRSEFIQPNLLLDGAHNPDSIAKLKSLLQEEFPKRPIHILF
                     AGLKRKPLADLLAQLDTFEVSVTTFDFPEANLLEDYPDKYPQVKDYRDWLQGSVTSDE
                     LFVVTGSLYFISEVRQYCLSNANIFKNIFP"
     gene            complement(6451..6891)
                     /locus_tag="VC122_000905"
     CDS             complement(6451..6891)
                     /locus_tag="VC122_000905"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014407485.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEP5640841.1"
                     /translation="MSSRYSRQQKPRKSGGVPTEHIKTGFTAFQKSIALIGSILSIIV
                     ATITITRALQPVPDKTSNDTPKESSNTIVKIIEKESSQGNQNHTGTSETTDDNKTTLP
                     SSSTTPSHNETTPTAPETPSATEGTQLPNATGAEDANTGLPTNS"
     gene            7126..8268
                     /locus_tag="VC122_000906"
     CDS             7126..8268
                     /locus_tag="VC122_000906"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012766813.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine desulfurase"
                     /protein_id="HEP5640842.1"
                     /translation="MIYFDNAATTIPYGEALKTYQEVATKIYGNPSSLHQLGTNASRI
                     LEASRKQIAGLLGVKSEEIFFTSGGTESANWAIKGIAFEKTAFGKHIIISAIEHPAVS
                     ESVKWLLTQGFEVSYAPVTTQGVVDVNALAELIRPDTILISIMAVNNEMGAIQPIRAI
                     SNLLANQPTITFHVDAVQAIGKIPLCDYMTNRVDLASFSGHKFHSVRGVGFLYKKAGK
                     RLNPLLSGGGQEQELRSTTENVAGIASMAKALRIVTEKQVSVLPKLTAMRDVIYKALS
                     AYPDVTVFSAQEGFAPNILTFGIRGVRGEVIVHAFEKYEIYISTTSACSSKAGKPAGS
                     LVAMGIPVKTAQTAVRISLDDDNDMGQVEQFLTIFKQIYDKTQKVR"
     gene            8280..9494
                     /gene="thiI"
                     /locus_tag="VC122_000907"
     CDS             8280..9494
                     /gene="thiI"
                     /locus_tag="VC122_000907"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002992657.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA 4-thiouridine(8) synthase ThiI"
                     /protein_id="HEP5640843.1"
                     /translation="MDYSEIMVRHGELSTKGKNRMRFINKLKNNIQDVLAPFPAIAVR
                     SDRDRTHVSLNGTDYQPIVEALKLVFGVQALSPVYKLEKSVPLLVTAVQDIMTSLYRD
                     GLTFKIATKRSDHAFELDSRELNSLLGGAVFEVLPNIQAQMKHPDVTLKVEIRDEAAY
                     ISYEEIKGAGGLPVGTSGKGMLMLSGGIDSPVAGYLALKRGLDIEVVHFASPPYTSPG
                     ALAKAQDLTRRLTRFGGNIQFIEVPFTEIQEEIKNKAPEAYLMTLTRRFMMRITDAIR
                     EQRKGLVIVNGESLGQVASQTLESMQAINAVTSTPIIRPVVTMDKLEIIEMAQAIDTF
                     DISIQPFEDCCTIFAPDRPKTNPKLGNAEKYEERFDIDGLVQRAVSGIVVTEITPEIV
                     NDEVENLIDALL"
     gene            9532..10824
                     /locus_tag="VC122_000908"
     CDS             9532..10824
                     /locus_tag="VC122_000908"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011184384.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CapA family protein"
                     /protein_id="HEP5640844.1"
                     /translation="MILLKSLYKGFFAAMKEENFEARCYNREKGGCVLIKTMQYKKTM
                     ATVVALIVALLLFGLIYDLLGVQKNKLAAQKSAQPKVKTARVVANGDILIHDILYMSA
                     RKADDTYDFTPYFEYVKDWISGADLAIGDYEGTISPDYPLAGYPLFNAPEEIAGALKN
                     TGYDVVDLAHNHILDSQLDGALNTKKVFHQLGIDSIGIYDKDRSKESFLIKNVNGIKI
                     AILGYSYGYNGMEATLSQEDYEKHMSDLDEAKIKKELQLAEKKADVTIVMPQMGTEYA
                     LEPTAEQKELYHKMIDWGADVVLGGHPHVIEPSETVIKGRQKKFIIYSMGNFISNQRL
                     ETVDDIWTERGLLMDLTFEKKDNKTKIKTVEAHPTMVLAKGKGIVGKEGFELYNYRTM
                     VLEDFIKGGKYHDKIDEETKAKVALAYQEINDLVNLKW"
     misc_feature    10929..11003
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00559"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="ribosomal protein L21 leader region; Derived by
                     automated computational analysis using gene prediction
                     method: cmsearch."
                     /db_xref="RFAM:RF00559"
     gene            11038..11352
                     /gene="rplU"
                     /locus_tag="VC122_000909"
     CDS             11038..11352
                     /gene="rplU"
                     /locus_tag="VC122_000909"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018374562.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L21"
                     /protein_id="HEP5640845.1"
                     /translation="MSTYAIIKTGGKQVKVEVGQAIYVEKIDAEAGAEVTFNEVVLVG
                     GDKTVVGTPVVEGATVVGTVEKQGKQKKVVTFKYKPKKGSHRKQGHRQPYTKVVINAI
                     NA"
     gene            11364..11690
                     /locus_tag="VC122_000910"
     CDS             11364..11690
                     /locus_tag="VC122_000910"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002985112.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosomal-processing cysteine protease Prp"
                     /protein_id="HEP5640846.1"
                     /translation="MIKAIFTRQKNGQLSSVTLTGHAGSGKHGFDIVCASVSTLAINF
                     VNSLEVLADCQALVDLNDVEGGYMAITIPPHDNKEEVQLLFESFLLGMTSLAKDSSKF
                     VNTQVI"
     gene            11718..12011
                     /gene="rpmA"
                     /locus_tag="VC122_000911"
     CDS             11718..12011
                     /gene="rpmA"
                     /locus_tag="VC122_000911"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018376869.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L27"
                     /protein_id="HEP5640847.1"
                     /translation="MLKMNLANLQLFAHKKGGGSTSNGRDSQAKRLGAKAADGQTVSG
                     GSILYRQRGTHIYPGVNVGRGGDDTLFAKVEGVVRFERKGRDKKQVSVYPVAK"
     gene            12369..13283
                     /locus_tag="VC122_000912"
     CDS             12369..13283
                     /locus_tag="VC122_000912"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011184386.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HEP5640848.1"
                     /translation="MNIQQLRYVVAIANNGTFREAASKLFVSQPSLSVSIKDLEAELG
                     FQIFNRTTSGTVLTSQGLVFYEKALEVVKSFDSFEKTFSQADLDQNEFSIASQHYDFL
                     PPLITAFSQQYDGHRVFRIFESTTIQILDEVAQGNSEIGIIYLNVDNQKGLFQRMDKL
                     GLEYVSLIPFTTHIYLSKTHPLANREALYLSDIQGLPAVRFTQERDEYLYYSENFVDT
                     SECPRIYNVSDRATLNGILERTNAFATGSGFLDHRSVNGIKVIPLADHIDNQMIYVKR
                     KDKNLSVAGATFVTILKDYFDKRRKALT"
     gene            13280..13738
                     /locus_tag="VC122_000913"
     CDS             13280..13738
                     /locus_tag="VC122_000913"
                     /EC_number="3.4.23.36"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010922145.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="signal peptidase II"
                     /protein_id="HEP5640849.1"
                     /translation="MKKRLFVLSLILLVALDQLSKFWIVSHIALGEVKPFIPGIVSLT
                     YLQNNGAAFSILQDQQWFFVVITVLVIGYAIYYLATHPHLNIWKQLALLLIISGGIGN
                     FIDRLRLAYVIDMVHLDFVDFAIFNVADSYLTVGVILLVICLWKEEDYGN"
     gene            13728..14618
                     /locus_tag="VC122_000914"
     CDS             13728..14618
                     /locus_tag="VC122_000914"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014635398.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RluA family pseudouridine synthase"
                     /protein_id="HEP5640850.1"
                     /translation="MEINVITSGQRLDKALADLSPLSRGQANDQIKQGLVLVNGQQKK
                     AKYTVQAGDVICFELPKEEVLEYQAQNIPLDIIYEDDTLAIINKPQGMVVHPSAGHPS
                     GTMVNALMYHIKDLSSINGVVRPGIVHRIDKDTSGLLMVAKTDAAHQALAEELKAKKS
                     LRKYLAIVHGNLPNDRGMIEAPIGRSEKDRKKQAVTAKGKEAVTRFTVLERFGDYSLV
                     ELQLETGRTHQIRVHMAYIGHPVAGDPLYGPRKTLSGHGQFLHAKTLGLTHPMTGKEM
                     IFTVEAPEIFQKVLKLLRKK"
     gene            15013..15534
                     /gene="pyrR"
                     /locus_tag="VC122_000915"
     CDS             15013..15534
                     /gene="pyrR"
                     /locus_tag="VC122_000915"
                     /EC_number="2.4.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009881107.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional pyr operon transcriptional
                     regulator/uracil phosphoribosyltransferase PyrR"
                     /protein_id="HEP5640851.1"
                     /translation="MKTKEIVDDVTMKRAITRITYEIIERNKQLDNVVLAGIKTRGVF
                     LARRIQERLHQLEGLDLPIGELDIKPFRDDMRVEEDTTLMSVDITGKDVILIDDVLYT
                     GRTIRAAIDNLVSLGRPARVSLAVLVDRGHRELPIRADYVGKNIPTSSVEEIVVEVVE
                     VDGRDRVSIIDPT"
     gene            15550..16809
                     /locus_tag="VC122_000916"
     CDS             15550..16809
                     /locus_tag="VC122_000916"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012560607.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="uracil permease"
                     /protein_id="HEP5640852.1"
                     /translation="MKDVIYDVEEVPKAGMLVGLSFQHLFAMFGATVLVPILVGIDPS
                     VALLSSGLGTLAHLSVTKFKIPAYMGSSFAYIAAMQLLMKTNGIGAVAQGAMTGGLVY
                     LIVALIVKVIGNDWIDKILPPIVVGPIVMVIGLSLASTAVNDVMLKNGNYNLTYLVIG
                     LVTLLSVIFFNIYGKGIVAIVPLLLGLLVGYVVALLVGVLTGQEIVDFTNVAQAKWFS
                     IPSVEIPFLTYGVKFYPSAILTMAPIAFVTMTEHFGHIMVLNSLTKRDYFKDPGLEKT
                     LTGDGFAQIIAGFLGAPPVTSYGENIGVMALNKIFSVYVIAGAAVIAALLSFIGKVSA
                     LIQSIPTPVIGGISVALFGVIASSGLKILIESKVDMDNKKNLLIASVILVSGIGGLML
                     QVNGLQISGVAFSTLLGIILYQVLPEK"
     gene            16870..17805
                     /locus_tag="VC122_000917"
     CDS             16870..17805
                     /locus_tag="VC122_000917"
                     /EC_number="2.1.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003048071.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate carbamoyltransferase catalytic
                     subunit"
                     /protein_id="HEP5640853.1"
                     /translation="MSVVNNRVALTNLVSMEALTTEEVLGLINRGSEYKAGKVVISDH
                     QKDLVANLFFENSTRTHKSFEVAEKKLGLTVLDFNADASAVNKGESLYDTVLTMSALG
                     TDICVIRHPEDDYYKELVESPTITASIVNGGDGSGQHPSQCLLDLLTIYEEFGRFEGL
                     KIAIAGDLTHSRVAKSNMQILKRLGAELYFYGPEEWYSEAFNAYGTYIAIDQIIKELD
                     VLMLLRVQHERHDGHQSFSKEGYHQAFGLTQERYQQLKDSAIIMHPAPVNRDVEIADS
                     LVEAPKARIVSQMANGVFVRMAIIEAILNGRNKNS"
     gene            17849..18931
                     /gene="carA"
                     /locus_tag="VC122_000918"
     CDS             17849..18931
                     /gene="carA"
                     /locus_tag="VC122_000918"
                     /EC_number="6.3.5.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011888994.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine-hydrolyzing carbamoyl-phosphate
                     synthase small subunit"
                     /protein_id="HEP5640854.1"
                     /translation="MTKRLLILEDGTIFEGEPFGADIDVTGEIVFNTGMTGYQESITD
                     QSYNGQILTFTYPLIGNYGINRDDYESISPTCKGVVVSEVSRLASNWRKQMTLDAFLK
                     IKGIPGISGIDTRALTKIIRQHGTMKATMADDGDSIQHLKDQLRATVLPTNTIEQVST
                     KTAYPAPGIGKNIVLVDFGLKHSILREFSKRQCNITVVPFNITAEEVLQLNPDGLMLS
                     NGPGNPEDLPEALDMIRGVQGKIPIFGICMGHQLFSLANGAKTCKMTFGHRGFNHAVR
                     EIATGRIDFTSQNHGYAVERSSLPDTLMVTHEDINDKTVEGVKHRDFPAFSVQFHPDA
                     APGPHDASYLFDEFLEMIDSWRCTSK"
     gene            19177..>22342
                     /gene="carB"
                     /locus_tag="VC122_000919"
     CDS             19177..>22342
                     /gene="carB"
                     /locus_tag="VC122_000919"
                     /EC_number="6.3.5.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001126118.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbamoyl-phosphate synthase large subunit"
                     /protein_id="HEP5640855.1"
                     /translation="MPKRKDIQKIMVIGSGPIIIGQAAEFDYAGTQACLALKEEGYKV
                     ILVNSNPATIMTDKEIADKVYIEPLTLEFVNRIIRKERPDAILPTLGGQTGLNMAMAL
                     SKAGILDDLEIELLGTKLSAIDQAEDRDLFKQLMQELDQPIPESTIVKTVDEAVTFAR
                     DIGYPVIVRPAFTLGGTGGGICSSEEELCEITENGLKLSPVTQCLIERSIAGFKEIEY
                     EVMRDSADNALVVCNMENFDPVGIHTGDSIVFAPTQTLSDIENQMLRDASLKIIRALK
                     IEGGCNVQLALDPYSFKYYVIEVNPRVSRSSALASKATGYPIAKLAAKIAVGLTLDEM
                     INPITGTTYAMFEPTLDYVVAKIPRFPFDKFEHGERQLGTQMKATGEVMAIGRNLEES
                     LLKACRSLEIGVCHNEMTSLSNISDEELVTKVIKAQDDRLFYLSEAIRRGYSIEELES
                     LTKIDLFFLDKLLHIVEIEQDLQMHVEHLESLKKAKRYGFSDQKIAEIWQKDESDIRA
                     MRHSHSLYPVYKMVDTCAAEFDAKTPYFYSTYELENESVQSNKESILVLGSGPIRIGQ
                     GVEFDYATVHSVKAIQKAGYEAIIMNSNPETVSTDFSVSDKLYFEPLTFEDVMNVIDL
                     EQPKGVIVQFGGQTAINLAQALSEAGVTILGTQVEDLDRAEDRDLFEKALKELGIPQP
                     QGQTATNEEEALEAAKKIGFPVLVRPSYVLGGRAMEIVENKEDLREYIRTAVKASPEH
                     PILVDSYIFGKECEVDAISDGKSVLIPGIMEHIERAGVHSGDSMAVYPPQQLSKQIQE
                     TIAEYTKRLAIGLNCIGMMNVQFVIKNEQVYVIEVNPRASRTVPFLSKVTGIPMAQIA
                     TKLILGQTLKDLGYEDGLYPQSQLVHIKAPVFSFTKLAQVDSLLGPEMKSTGEVMGSD
                     TSLEKALYKAFEANNSHLSEFGQIVFTIADDSKAEALSLARRFKAIGYQIMATQGTAA
                     YFAEQGLSACLVGKIGDAANDIPTLVRHGHVQAIVNTVGIKRTADKDGQMIRSSAIEQ
                     GVPLFTALDTAKAMLTVLESRCFNI"
BASE COUNT         6860 a         3827 c         4859 g         6796 t
ORIGIN      
        1 gattcttttg tcgaaaagaa gctgacagca ggggtacaat tattagatga aaaagatttt
       61 tctaatataa aggaaaaaaa tcagttggtt caactggtga ccaagtccaa tcgtcccatt
      121 ggaacagcat atatctctaa gcaaaataaa ggaattggtt ggtatctagg accagaaaaa
      181 atcgacttgt ctatctctta ttttgttagt ctattttcag ttgctaaggc caaaagacaa
      241 gattttgctc aatcggacga gaccaatgct tatcgccttt ttaatcaaga aggagacggt
      301 tttggtggag taacaattga tctttataaa gattttgcag tcttttcatg gtacaatgcc
      361 tttgtctatg acaaaaaaga aatgattatg gaggcttttc aacaggtttt tcctgaagtc
      421 aaaggagctt atgaaaagtg tcgctttaaa ggtccagata ctgaaactgc tcatctgtat
      481 ggtgagctgg cacaagaaac cttttcgatt ttagaaaatg gcatagccta tcaagtcttc
      541 ttgaatgagg gcttaatgac aggtgttttt ctagaccagc atgatgttag aagagcacta
      601 gtagatggtt tagctatggg caagtccctt cttaacctat tttcctatac ggctgccttt
      661 tctgtagcag ctgctatggg tggtgctatc gagaccactt ctgtggactt ggctaaacga
      721 tcgagagaat tatcactagc tcattttgag cataaccagt taaacttagc ctcacatcat
      781 tttgtggtga tggatgtttt tgaatacttc aaatatgcca aacggaaaaa attaatcttt
      841 gatgtgattg tgattgaccc accaagcttt gctagaaata aaaaacaaac tttctctgtg
      901 agcagggact accacaaatt aatcacagag gccttggaca tattgagtcc taagggaact
      961 attatagctt caacaaatgc tgctaatatg actgttagcc agttcaaaaa gcaaattatc
     1021 aaaggatttg gaagtcgtcg tcctgagtct atgactctcc agcagcttcc gagtgacttt
     1081 actatcaata aagcagatga aagaagtaac tatttaaagg tatttactat aaaggtacga
     1141 gaatgagaat tgtagcacca gtcatgccaa gacattttga cgaggctcaa gccattgata
     1201 tttctaaata tgaagatgtt aatttaattg aatggcgagc agactttctt cctaaggatg
     1261 agattgtcgc agtagcacca gctatttttg aaaaatttgc cggaaaagaa attatcttta
     1321 cccttcggac cgttcaagaa ggggggaata ttaccctttc aagtcaggag tatgttgata
     1381 ttatcaaaga aatcaatgct atttataatc cagactatat tgactttgag tattttacgc
     1441 acaagtcagt atttcaagaa atgcttgatt ttccaaatct tattttgtct tatcacaact
     1501 ttgaagagac tcctgaaaat ttaatggaag ctttttcaga aatgaccaag ttggcaccgc
     1561 gtgtggttaa aattgctgtg atgccccaga gtgaacaaga tgttctagat ttgatgaact
     1621 ataccagagg ctttaagacg ctcaatcctg agcaagaatt tgcaactata tctatgggga
     1681 aattaggaag attgtctcgt tttgcgggag atgtcattgg ctcttcctgg acatacgttt
     1741 cattagatca tgttagcggt ccaggtcaag tgactctcaa tgatatgaaa cgtattattg
     1801 aggtcttaga aatggatatc tctaactagt gaatggtcta aaaatattat ttctacaata
     1861 gttaagaaat gatgttttta gattaatgtt tttaaaataa gtggaaccaa aaaggagaaa
     1921 aattgcgtta tttaacagca ggagaatctc atgggccatc cttaacagct attattgaag
     1981 gtataccagc aggattaact cttcatccag cggatattga tcacgaatta cagcgacgtc
     2041 aaggaggtta tggccgtggt gccagaatgt ctattgagac tgatcgggtt caaatatcct
     2101 caggagttcg tcatggcaaa acaacagggg cccccattac cttaacggta attaataagg
     2161 accaccaaaa atggctagat gtcatggctg taggagacat tgaagagact ctcaagttaa
     2221 agcgtcgtgt taagcatcct agaccaggac atgcggattt ggtaggaggt attaaatatc
     2281 attttaacga tttaagagat gctttagagc gttcctcggc tagagaaaca acgatgagag
     2341 tggctgtagg cgctgttgct aaacgtatct tagctgaact tggaattgat atgttacatc
     2401 atatcttaat atttggtggt ataacgatca caataccttc taagctctct tttagagagt
     2461 tgcaagaaag ggctctccat tcagagttat ctattgttaa tccaaagcaa gaagaagaaa
     2521 ttaaaacgta tattgataag ataaaaaaag aaggggatac tattggtggt attatcgaaa
     2581 cgatagttca aggggtgcca gcaggtcttg gatcttatgt ccaatgggat aaaaaattag
     2641 atgccaagct ggctcaagca gttctttcca ttaatgcttt taaaggggtg gaatttggag
     2701 ttgggtttga tatgggattc caaaagggct ctcaggtaat ggatgaaatc acctggacac
     2761 ctacccaagg ctatggtcgt caaacaaacc acttaggtgg ctttgaaggt ggcatgacca
     2821 caggacaacc tcttgttgtt aaaggggtta tgaagcccat tcctactttg tataaaccac
     2881 tcatgtcggt agatattgat agtcatgaac cttataaagc aacggtggaa cgctctgatc
     2941 cgacggcctt accagctgca ggggttatca tggaaaatgt ggtggcgacg gtgcttgcca
     3001 aagagatttt agagactttt tcttctacta ccatgtctga attgcaaaaa gctttttcgg
     3061 actatcgtgc ctacgtcaag caattttaga tagcgagata ctggctttta tggtatcata
     3121 caggtattaa tgaacaaaag gagactgtca tgtcacaaga aatttatgac tatgctaatc
     3181 aacttgaaag agcagttcgt gctctgccag aataccaaaa agtgttagaa gtaaaagagg
     3241 ccattcaagc agacgctagt gctagcgaac tgtttgatga gtttgtcgct atgcaagaaa
     3301 aaatccaagg gatgatgcaa tcaggccaaa tgccaacggc tgaagaacaa acaagcattc
     3361 aggaacttag ccaaaagatt gaagctaatg accagctaaa agcttatttt gaagcgcagc
     3421 aggccttatc tgtttacatg agtgatattg agcgtattgt atttgctccg ttgaaagatt
     3481 tagtcaagta agccaattaa gataggaaaa aaaacagatt gctggcagct cgtcaggatc
     3541 tgttttttag tgtttatttt ctttgtatca gaaagttgag cctctttctt atttgtgata
     3601 tagtcaaata aattgaccaa aatagcaggc cagtagtggc tttaatgaag gcaagttgtc
     3661 tgctaactgg ctatgttaaa acatcagtat tagaaagtaa aaggttataa aaatggttat
     3721 tccttatgat tatattgtta ttggtggagg gagtgcaggt attgcttctg cgaacagggc
     3781 agccatgcat ggggccaaag tattgttagc tgaaggtaaa gagattgggg gaacctgtgt
     3841 taacctaggc tgcgttccta aaaaagtcat gtggtatggc gctcaagtag cagatatttt
     3901 agggacttac gctaaggatt atggatttga ctttaaagac aaagcatttg attttaaaca
     3961 attgaaagca aatcgtcaag cttatattga tcggattcat gcttcttatg agcgaggctt
     4021 tgagcagaat ggtgttgacc gtatttatga ttatgctgtt ttcaaagatg ctcatacggt
     4081 agaaattgca gggcagcttt ataccgcacc gcatattttg attgccacag gaggacatcc
     4141 tgtttttcca gatattgagg gggcacaata cggtatcagc tcggacggct tttttgcctt
     4201 agatgaggta ccaaaacgta cggctgtggt tggggcaggc tatattgctg tggaacttgc
     4261 cggtgttttg catgctttgg ggtcgaagac ggatttgttt atccgtcacg atcgaccttt
     4321 aagaagtttt gacaaagcca ttgtggatgt tttagttgag gaaatggcag ttggtggtcc
     4381 ttgcttacac cctcattcag aagtggcaaa ggttgtcaaa aatactgatg agtccttgac
     4441 cctttacctc aaagatggcc aagaagttga ggttgaccag ctcatttggg ctatcggtcg
     4501 caagcctaat ctagaaggtt ttggtcttga caaaactgga gtgacgttaa atgataaagg
     4561 ttatattgag acagatgcct atgaaaacac gtctgtcaaa ggtatttatg ccgtgggaga
     4621 tgttaatggg aaactggctt taactccagt tgctgtggca gctggacgtc gcctatcaga
     4681 gcgtcttttc aatggcaaaa cagacgagaa attggactac caaaacgttg ctactgtgat
     4741 ttttagtcac cctgttattg gctctgttgg cttgtcagaa gaagcagcag tcaaacagta
     4801 tgggcaagaa gcagttaaaa catatcaatc acgttttacg tctatgttta ctgctatcac
     4861 taaccatcgc caaccttgtc tgatgaagtt ggtaacagtc ggagataccg aaaaaatagt
     4921 tggtcttcac ggaatcggct atggggttga tgaaatgatt cagggctttg cagtagccat
     4981 caaaatggga gcaaccaagg cggattttga taacaccgtt gctattcacc caactggctc
     5041 agaagaattt gtcaccatgc gctaaaaaat gtagcctaag gcaataaaaa atgatgttaa
     5101 cagtttttga ggaaagtctt ctaaactgct agcatcgttt tttgttaggt gattagggga
     5161 atatgttttt aaagatattt gcgttgctca gacaatattg ccttacctcg gagataaaat
     5221 aaagtgaccc tgtaacaaca aagagttcat cagatgttac ggaaccttgt aaccagtctc
     5281 tgtaatcttt tacctgtggg tatttatcag gataatcctc taaaagattt gcttcaggga
     5341 aatcaaaagt cgtgacagat acttcaaaag tatctagttg agccagcaag tcggctaaag
     5401 gcttacgttt gagacctgca aagagaatat gaataggacg tttgggaaat tcttcttgca
     5461 gtagagactt taatttagca atggaatctg gattgtgagc gccgtctaat agaagattag
     5521 gttgaataaa ttcggaccgg ccaggccaag ttgttgcctc aattcctttt tgaatcgttt
     5581 ttggagatat tttaggaaag gattttctaa gtaattgagc tgtcataatg gctaaacagg
     5641 cattcgcttt ttgatgttga cctaatagtt tgagacggag tttggttaaa ttcaagttat
     5701 cgtgttgaaa ggaaaacgtc ttttctgaag gattgagcaa aaaatctttt cctaattgat
     5761 ataagcgtga accggctttt tgggtaactt gttgaaagac ttgactagcc tcctgctcaa
     5821 gttgaccgat aataacgggt tctttagctt tgataacttc tgctttttgg tgagcaattt
     5881 ctgctaggct ataacctaaa cgttcttggt gatcaaaact aatagaaggg catacgacgg
     5941 ccagagcatg aaaaacattg gttgcgtcgg ttttcccacc aatccctgcc tcaataatgg
     6001 caatatcaac aggtttaaga ttggcaaagt agcaaaacat tatgagagtc actaactcaa
     6061 actcggtagg tctatcccaa tttgtttcaa gggacatttt agtgagaatg ggtctaatca
     6121 cgctcacaca ttgcgccaat tctaggtctg aaatgggctg gccattaagg caaatccgat
     6181 cgtgaaaaga gataataaaa ggagatgtaa aggttccgac ctgataacca gaagttgaca
     6241 ggatatgctg taagtaggct gtcacagagc ccttgccgtt agttccaaca acgtgaatag
     6301 cagggaattt gtcttgtggc cggcctaatg cctcaagtaa ccaagccatt ctggccaaat
     6361 cggtcttgcg accattagcc ttaaaggtat gaatccagga aatgctatca taggtagtca
     6421 ttactatttc ttgtccttct aacaatgctt ttaagagttc gtagggagac cagtattagc
     6481 atcctcagct ccagttgcat taggaagttg cgtcccttca gtggctgatg gtgtctcagg
     6541 agctgtagga gttgtctcgt tgtgagaagg tgtggtagaa gatgacggaa gagttgtctt
     6601 attatcatct gtggtttctg aagtacctgt atggttttga ttaccctgag aagactcttt
     6661 ttcaataatc ttgacaatgg tattactcga ttctttgggt gtatcattag aggtcttatc
     6721 tggtacaggc tgcagtgctc ttgtaatggt aatggttgcc acaatgatac tcaaaatact
     6781 accaataagt gcaatggatt tttggaaagc tgtaaaacct gttttaatat gctcggtagg
     6841 gacacctcct gatttacgag gtttttgttg acgggaataa cgtgatgaca tatttttctc
     6901 ctttaaaatc atcgtaatgc caagattaag gaggaggaaa tagaagtcct agctcctctt
     6961 taacacttca tctatcagta taatctagaa aaaaagtgaa gtcaacggtt taccttatgt
     7021 ctaggacaag gaagtccttt gggcttattg taatacgaat attcttgatt tcattttagc
     7081 tattttgtga tagaataaag tattgagtaa aaatgaaggt gacacatgat ttattttgat
     7141 aatgcagcca caaccatacc ttatggagag gctcttaaaa cttaccaaga agtagcaaca
     7201 aaaatatatg gcaatccctc aagtctgcac caattaggga caaatgcgtc tcgcatttta
     7261 gaagcttccc gtaaacagat tgctggtttg ttgggtgtta agtcagaaga aatctttttt
     7321 acgtctggtg ggactgaaag tgctaattgg gctattaagg gaattgcttt tgaaaaaact
     7381 gcttttggca aacatattat tatctcagcc attgaacacc ctgccgtgag tgaaagtgtc
     7441 aaatggcttc tcacacaagg attcgaagtt tcttatgctc cagtgacaac acagggagtt
     7501 gttgatgtga atgctttagc tgagctgatt agaccagaca ccatcttaat ctcgattatg
     7561 gctgttaata atgaaatggg agctatccaa ccgattagag ccatttcaaa cctattggct
     7621 aaccaaccaa cgatcacctt tcacgttgat gctgttcaag ccattggtaa aataccactc
     7681 tgtgattata tgactaatcg tgtggatttg gcttcttttt cagggcacaa atttcatagt
     7741 gttcgtggtg ttggcttcct ttataaaaaa gcaggcaagc gactcaatcc tctcttgtca
     7801 ggcggcggcc aagagcagga gttacgttca accactgaaa atgttgcagg aattgctagc
     7861 atggccaaag cattaagaat agtcactgaa aaacaagtga gcgtcttacc taaactaaca
     7921 gcgatgcgag atgtgattta caaagctttg tccgcttacc cagatgtgac agtattttca
     7981 gctcaagaag gctttgcgcc caatattttg acctttggga ttagaggggt tcgaggagaa
     8041 gttattgtcc acgcctttga aaaatatgag atttatattt cgaccacaag tgcatgttcg
     8101 tccaaagcag gtaagccagc aggaagccta gtggctatgg ggattcctgt aaaaactgcc
     8161 cagacagccg ttcgtattag tctagacgat gacaacgata tgggacaagt agagcagttt
     8221 ttgaccatat tcaaacagat ttatgataaa acacagaaag ttaggtaaga ttaggtaaga
     8281 tggactattc agaaattatg gttcgccatg gtgaactgtc aactaaaggg aagaatcgca
     8341 tgcgattcat taacaaactt aaaaacaata ttcaagacgt actggctcct ttcccagcca
     8401 ttgctgtccg ttcagatcgt gataggaccc atgtgtctct aaatggcaca gattatcagc
     8461 ctattgtaga agctttaaag ctggtgtttg gtgttcaagc tctatctcct gtttataaac
     8521 ttgaaaaaag tgtccctctt ttggtaacag ctgttcaaga tatcatgact tctctttatc
     8581 gtgatggact gacgtttaag attgccacta agcgtagtga tcacgcgttt gaattggata
     8641 gtcgtgagct taacagtctt cttggtggag ctgtttttga ggttttacca aatatccaag
     8701 cccagatgaa acatcccgat gtgactctca aagtcgaaat tcgtgatgaa gcagcttaca
     8761 tttcttatga agagattaag ggagcaggcg ggttaccagt aggaacgtca gggaaaggga
     8821 tgctgatgtt atcgggcggt attgattcac cagtagctgg gtatttagcc cttaaacgtg
     8881 gcctcgatat tgaagttgtt cattttgcta gccctccgta caccagtcca ggtgccttgg
     8941 ctaaagccca agaccttact cgtcggttaa ctcgttttgg tggaaatatc caatttatcg
     9001 aggttccttt tacagaaatt caagaagaaa ttaaaaataa ggcaccagag gcttacctta
     9061 tgacattaac ccgccgcttt atgatgcgca tcacagatgc cattcgtgag caacgtaagg
     9121 gtcttgttat tgttaacggt gagagcctag gtcaggttgc tagtcaaacc cttgaaagca
     9181 tgcaggccat taatgcggta acatcaaccc caattattag accagtcgtg acaatggata
     9241 aacttgaaat tatcgagatg gctcaggcga ttgatacttt tgacatttct attcaaccgt
     9301 tcgaagattg ctgtactatt tttgcaccag accgtcctaa aaccaaccct aaactaggta
     9361 atgctgaaaa atatgaagaa cgctttgata ttgatggtct tgtccagcgt gctgtgtcag
     9421 gaattgttgt cacagaaatc acacctgaaa tcgtcaatga tgaggttgaa aatctcattg
     9481 atgctttact ttaaaagaaa agatcaagtg taaatctttt agtagtcagt aatgatacta
     9541 ttgaaaagcc tctataaggg cttttttgct gcaatgaagg aggaaaattt tgaggcaaga
     9601 tgttataata gagaaaaagg agggtgtgtc ttgattaaaa ctatgcaata caaaaaaacg
     9661 atggcaacag ttgttgctct tatcgtcgca cttttgctat ttggcttgat ttatgattta
     9721 cttggggttc aaaaaaataa gctagcagct caaaaatctg ctcagccaaa ggtcaaaaca
     9781 gcgcgtgttg tagctaatgg cgacatttta attcatgata ttctctacat gagtgctaga
     9841 aaagctgacg atacctatga tttcactcct tattttgaat atgttaagga ctggattagc
     9901 ggtgctgact tagctattgg agattatgaa gggaccatta gcccagatta tcccttagca
     9961 ggctatccct tattcaatgc tccagaagag attgcaggtg ctctgaaaaa tactggctat
    10021 gatgtagtcg atttagccca taatcatatc ttagattccc aattagacgg tgcccttaac
    10081 actaaaaagg tttttcatca attaggtata gacagtattg gcatctatga caaggatcgt
    10141 tcaaaagaat ccttcttaat taaaaatgtc aatggtatca aaattgcaat tttaggttat
    10201 tcttatggtt ataacggcat ggaggccaca cttagccaag aagactatga gaaacacatg
    10261 tctgatttag atgaagctaa gataaaaaaa gaacttcagc tagctgaaaa aaaggctgac
    10321 gtgaccattg tcatgccaca aatgggaaca gaatatgcct tagaaccgac agcagagcaa
    10381 aaagaacttt accacaaaat gattgattgg ggagctgacg tcgtcctagg aggccatccg
    10441 catgttattg aaccttcaga gacagttata aaaggtaggc aaaagaaatt cattatttac
    10501 tctatgggaa attttatttc aaatcagcgg cttgaaaccg tagatgatat ctggactgag
    10561 cgtggcctat tgatggacct tacttttgag aaaaaagata acaaaacaaa aattaaaaca
    10621 gttgaggccc atccaaccat ggttttagcc aaaggtaaag gcattgtggg taaagaaggt
    10681 tttgaactat acaattatcg aacaatggtt ttagaagatt ttatcaaagg tggaaaatac
    10741 cacgacaaga ttgatgagga aaccaaagca aaagtagcac ttgcttatca ggaaattaat
    10801 gatttagtta acctgaaatg gtaatgtttg gtggtttagc atctaacgat aaaaaataga
    10861 gattaaactt aatttttcct tgataaaaag cagttatggt gctaaaatag taacgttgac
    10921 taaatgcaca tcctgtgcaa ccgcgcgaaa atcgttgtca agtagaccaa gtctcacgat
    10981 tccaggcgag tcttcacaga gggaaacctc aaacaaaaat tttataggag gtgcatcatg
    11041 agcacatacg caatcatcaa aactggtgga aaacaagtta aagttgaagt aggtcaagca
    11101 atctacgttg agaaaattga cgctgaagct ggcgcagaag tcacttttaa cgaagttgtt
    11161 cttgtaggtg gtgacaaaac tgtagttggt actccagttg ttgaaggagc tactgtcgtt
    11221 ggaactgttg aaaaacaagg aaaacaaaag aaagttgtaa cattcaaata caaacctaaa
    11281 aaaggtagcc accgtaaaca aggtcatcgt caaccttaca ctaaagttgt catcaacgca
    11341 atcaacgctt aatttactag cttatgatta aagcaatttt tactcgccaa aaaaatggtc
    11401 aactgagcag tgttacactg acaggtcatg ctggtagtgg taaacatggc tttgatattg
    11461 tttgtgcctc tgttagtact ctagccatta actttgttaa ttctcttgag gtgttagcag
    11521 attgccaagc attagtagat cttaatgatg ttgaaggtgg gtatatggct attacaatac
    11581 caccacatga caacaaggag gaagttcaat tattatttga atcttttctt ctaggaatga
    11641 ctagtttggc gaaggattct tcgaaatttg tcaacactca ggtcatctaa atttaattta
    11701 agagaggaaa caacattatg ttaaaaatga atcttgctaa cttgcaactt ttcgcccaca
    11761 aaaaaggtgg aggttctaca tcaaacggac gtgactcaca agctaaacgc cttggtgcta
    11821 aagctgctga cggtcaaact gtttcaggtg gatcaatcct ttaccgtcaa cgtggaactc
    11881 atatctaccc aggtgtaaac gtaggccgtg gtggagatga cacacttttt gctaaagttg
    11941 aaggtgttgt acgtttcgaa cgtaaaggac gtgataaaaa acaagtttct gtttacccag
    12001 tagctaaata attgtttaac atcgcattat ataaggcttt ccaggtgtct ggaaagccga
    12061 tttttttatt ttggtactca tgttgctatc catattttaa aaatcaatgt aggaagagag
    12121 cttgtcagca gttttatctt ttgaggattt agtaacatga gcgtagatgt tcatggtagt
    12181 ttggatagtt tcatgaccaa gtgtattgga ttttcattgt ttgtttctat cctactaaag
    12241 attgtagttg cttatttaga gcaatagttc taattgtaaa aagtcagcat aataaaacaa
    12301 tgtcataaga tgttgttata acgggcttta tggtataatt aaggatactc attttaggaa
    12361 ggaaagcaat gaatattcaa caattacgct atgtggtagc catcgctaat aatggaactt
    12421 ttcgagaagc tgcctctaaa ttgtttgtca gtcagcctag cttatcagtt tctatcaaag
    12481 atttagaggc ggaattaggg tttcaaattt ttaatcggac tacttcagga acagtcttga
    12541 caagtcaagg actagttttt tatgagaaag ctttagaagt agtcaaatct tttgattctt
    12601 ttgaaaaaac cttttctcaa gctgatctgg atcaaaatga attttctatt gctagccaac
    12661 attatgactt tttgccacct cttattacag ctttttcgca acaatacgat ggtcatcgtg
    12721 tctttcgtat ctttgaatca acaactattc aaattttaga tgaggttgct caaggaaaca
    12781 gtgaaattgg tattatttac ttgaatgtag acaatcaaaa aggtctcttt cagcggatgg
    12841 ataagctggg cttggaatat gtttctctaa ttcctttcac tactcatatt tatctttcaa
    12901 aaacgcatcc attggctaat cgcgaagcgc tttatttgag cgatattcaa ggattacctg
    12961 cggtaaggtt tacccaagaa agggacgagt atttgtatta ttctgaaaat tttgtggata
    13021 ctagtgaatg ccctcgtatt tataatgtgt cagatagggc aaccttgaac ggtattttag
    13081 agagaaccaa tgcctttgcg acaggatcag gttttctcga tcatcggagt gttaatggga
    13141 ttaaagtgat acctctagct gatcatattg acaatcaaat gatttatgtc aaacgaaaag
    13201 ataaaaacct ttctgttgct ggggccactt ttgtgactat tttaaaagac tattttgata
    13261 aaagaaggaa ggccttaaca tgaaaaaacg attgtttgtg cttagcttga tcctccttgt
    13321 agctttggat caacttagta aattttggat tgtttctcat atagcgcttg gagaagtgaa
    13381 accctttatc ccaggtatcg tcagcttgac ttacttgcaa aacaatgggg ccgccttttc
    13441 catattgcag gaccagcaat ggttctttgt tgtcataacg gttttagtta tcggttatgc
    13501 tatttattac cttgctactc atccccattt aaatatctgg aaacaattag ctctcttgct
    13561 tattatttct ggtggaatcg ggaattttat tgatcgtttg cgtttagctt acgtgattga
    13621 tatggttcat ttagactttg tggattttgc catttttaat gtggcagatt cataccttac
    13681 cgttggtgtc atattattag tgatatgttt atggaaagaa gaggattatg gaaattaatg
    13741 tgataacatc ggggcaacgc ttggataagg cactagctga tttaagtccc ctgtcacgtg
    13801 gtcaagccaa tgaccaaatt aagcaaggcc ttgttttagt taatggtcag caaaaaaagg
    13861 ctaaatatac ggttcaagca ggagatgtga tttgttttga actccctaaa gaagaagtct
    13921 tagagtacca agctcagaat attcccttgg acattatcta tgaagatgat actctcgcca
    13981 ttattaacaa gccacaggga atggtagttc acccttcggc gggacaccct tcaggaacaa
    14041 tggttaatgc cttgatgtat catatcaagg atttgtcctc aatcaatggt gtggttcgtc
    14101 caggaattgt tcatcggatt gataaggaca cctcaggtct cttaatggta gctaaaacag
    14161 atgcagctca tcaagccttg gcagaagaat taaaggctaa aaaatctctc agaaaatacc
    14221 tcgccattgt ccatggaaac ctaccgaatg atcgcgggat gattgaagct cctataggtc
    14281 gtagcgaaaa ggaccgtaaa aaacaggctg ttacagcaaa aggcaaagaa gcagtgacac
    14341 gttttacagt gctagaacgt tttggcgatt acagtctagt ggaattacaa ctggaaactg
    14401 gtcgaacgca ccaaattcgt gttcatatgg cctatattgg tcatcctgtt gctggggacc
    14461 ctttatatgg accacgtaag acactatcag gtcatggcca atttcttcat gcaaaaacat
    14521 tgggattaac gcatcctatg acaggaaaag aaatgatatt tactgttgaa gctcccgaaa
    14581 tttttcaaaa ggtgttaaag ctgctaagaa aaaaataaac agcgaggagt agccattatt
    14641 tttagtctta tatctttgtg taaagttttt aacattataa aaaatactaa aaagcattta
    14701 cgataagcca ggaaatatga gaagtctttt atgttattgg gatatgcaac atatctgtta
    14761 gagatcttta tttaaactct taaagaaaag agatattcca aatagatgct aggtattgtc
    14821 ttttttactt gtttttcttt tctttttttg ctatactgat tgaaataaaa tattccttta
    14881 aaacagtcct gtgaggctgt caaggagacg gattaaaaac atgcactatg atacttaatt
    14941 gtgctatatt ttgctgaatc tccttgtttc caaggagatc tttttatttg aatttttaga
    15001 ggaggtttca acatgaaaac taaggaaatt gttgatgacg tgaccatgaa acgcgccatt
    15061 acccgtatta cttatgaaat tattgaacgt aataaacaat tagacaatgt ggttttggca
    15121 ggcatcaaaa cgagaggtgt ttttctagct cgtcgcattc aagaaagact gcatcaatta
    15181 gaagggttgg atttaccaat tggtgagtta gacatcaagc cttttagaga tgacatgaga
    15241 gttgaggaag acacaactct gatgtcagta gatattacag gcaaggatgt gattttgatt
    15301 gatgatgtgc tttatactgg tcgtaccatc cgcgcggcca tcgataactt agtgagttta
    15361 ggtcgaccag cccgtgttag tctagctgtt ttggtggatc gtggccatcg ggaattaccg
    15421 attcgcgcgg attacgttgg gaaaaatatt ccaacaagca gtgtagaaga aattgttgtt
    15481 gaggttgtcg aagttgatgg ccgtgatcgt gttagcatta ttgacccaac ataagaagga
    15541 ggttttactg tgaaagatgt catttatgat gtggaagaag tgccaaaggc aggtatgtta
    15601 gttggtttgt cctttcagca tttatttgcc atgtttgggg cgacggttct tgttcctatt
    15661 ttagttggaa ttgacccgtc tgtggccctc ctatcgagtg gtttaggaac acttgcacat
    15721 ttatcggtaa ccaaatttaa aattccagcc tacatgggct caagttttgc ttatatagca
    15781 gctatgcagt tactgatgaa gacaaatggt attggtgctg tagctcaagg ggctatgaca
    15841 ggaggtctcg tttacctgat tgtagctttg attgttaaag ttattggcaa tgattggatt
    15901 gataagattt taccaccgat tgtagttggt ccgattgtta tggtcattgg tctaagtcta
    15961 gcttctacag ctgttaatga tgtcatgtta aaaaatggga actataatct gacttatctt
    16021 gttattgggt tagtcacctt attatcagtt atttttttca atatttatgg taagggaatt
    16081 gttgctattg taccgctttt attagggcta ttagttggtt atgttgtagc ccttttagtg
    16141 ggtgttctca ccggtcaaga aattgttgat tttaccaatg tggctcaggc caaatggttt
    16201 agtatcccat cagtggaaat tcctttctta acctacgggg ttaaattcta cccaagtgcc
    16261 attttaacta tggcacccat tgcctttgtt acaatgacag aacattttgg acacattatg
    16321 gtcttaaata gtttgacaaa aagagattat ttcaaggatc caggacttga aaagacttta
    16381 actggtgatg gttttgcgca aatcattgct ggcttcttag gggcacctcc ggtcacttct
    16441 tatggtgaaa acatcggtgt aatggctctg aataaaatat tctcggtcta tgttattgca
    16501 ggtgccgcag tgattgccgc tctcctcagt tttatcggta aggtatcagc cttaattcaa
    16561 tctattccaa caccagttat tggaggtata tcagttgcct tgtttggtgt aatagcttct
    16621 agcggtttga aaatcttgat tgaatccaaa gttgatatgg ataataagaa aaacttattg
    16681 attgccagtg tcattttggt ctctgggatt ggaggattga tgcttcaagt aaatggtctt
    16741 caaatttcag gtgtcgcttt ctcaacactt cttggcatca tcttatatca ggtacttcct
    16801 gaaaaatagg aagaattgtt gttagaccac ttacatagtc atcagtcgtt agtgataaag
    16861 gggataatca tgtcagttgt aaataatcgc gttgcactta ccaatttagt ttcaatggaa
    16921 gcattaacca ccgaagaagt tttaggtctt atcaaccgtg gttcagaata taaggctggt
    16981 aaggttgtta tttcggatca tcaaaaggat cttgttgcca atttattctt tgaaaactcc
    17041 acccgaaccc ataagtcttt tgaagtggct gagaagaaac tcggcttgac agttcttgat
    17101 ttcaatgctg atgcaagtgc ggttaacaag ggagaaagtt tgtatgatac tgtgttaacc
    17161 atgagtgcac ttggaacaga catctgtgtg attcgtcacc cagaagatga ttattacaag
    17221 gaattagtag aaagtccaac tattacagct tccattgtca atggaggaga tggttctgga
    17281 caacacccga gtcaatgttt acttgactta ttaaccatct atgaagaatt tggacgcttt
    17341 gaaggcttaa agattgcgat cgctggtgac ttaactcact ctagggtggc aaagtccaac
    17401 atgcaaatcc tcaaaagatt aggagcagag ctttatttct atggacctga agaatggtat
    17461 tcagaagcct ttaatgctta cggcacttat attgctattg accagattat taaggaattg
    17521 gatgttctta tgttgctgcg tgtacagcac gaacgtcatg acggccatca gagtttttca
    17581 aaagaaggtt accatcaagc gtttggattg acacaggaac gttaccaaca attaaaagat
    17641 tctgctatta ttatgcatcc agctccagtt aatcgggatg tggaaattgc agatagtttg
    17701 gtggaagctc ctaaagcgag aattgtatct cagatggcta atggtgtctt tgttcgcatg
    17761 gctattattg aagccatttt gaatggtaga aataaaaact catgatgaaa gattaaagca
    17821 tgtttagaag gtaggaaaag gtaggaaaat gacaaaacga ttacttattt tagaagacgg
    17881 cactattttt gaaggagaac cctttggtgc tgatattgat gtgactggtg aaattgtctt
    17941 taatacagga atgacaggct accaagaatc catcactgac caatcctaca atgggcaaat
    18001 tttaaccttt acctatcctt tgattggaaa ttatgggatt aatcgagatg actatgagtc
    18061 catttcaccc acctgtaaag gggttgttgt gtctgaagtc agtcggttgg caagtaattg
    18121 gcgcaagcaa atgacgctag atgcgttctt gaaaataaaa gggattccag ggatttcggg
    18181 aattgatacg agagctttga ccaaaattat tcggcaacac ggtaccatga aagctacgat
    18241 ggcagatgat ggagatagta tccagcattt aaaagatcaa ctgcgtgcaa cagtcttacc
    18301 gactaacacc attgaacaag tgtcaactaa aacagcttat cctgcaccag ggatcgggaa
    18361 aaatattgta ttggttgatt ttggtttaaa acattctatt ttaagagaat tttctaaaag
    18421 acaatgtaat atcaccgttg tccctttcaa catcactgca gaagaagtgc tccaactaaa
    18481 tccggatggt ttgatgctat ctaatggccc aggaaaccca gaagatttgc cagaggcttt
    18541 agacatgatt cgtggggtac aaggtaagat tcctattttt ggtatttgta tgggacatca
    18601 actctttagt ctagcaaatg gtgccaagac ttgtaaaatg acctttggtc atcgtggctt
    18661 taaccatgcc gttagagaaa ttgcaacggg aagaattgat tttaccagtc aaaaccatgg
    18721 ttatgccgtt gaacgtagta gtctacctga tactttaatg gttacgcatg aagacattaa
    18781 cgataagacg gtcgaaggag tgaaacatag agattttcca gctttttcag tccaatttca
    18841 ccctgatgcg gctccaggac ctcatgatgc cagctactta tttgacgaat ttttggaaat
    18901 gattgattct tggagatgta cgagtaaata gtaaatcagt tgcaatttca cgactatatc
    18961 acctcttgtt actctattta ttaacattta cagttgattt agtaccctat caaaaatatt
    19021 atttaatgtt gctctgtgag gcaacaaata gagaaaggaa ggcaaggaga acacaagtaa
    19081 ataatttagt aaaaagttag gttttcaaag agataaagct cttattgaga agtgctgact
    19141 ttaccgcttc ttgtggtatt cttggtatca ttaattatgc caaaacgaaa agatattcaa
    19201 aaaatcatgg ttatcgggtc tggcccgatt attattggac aagctgctga atttgattat
    19261 gcagggactc aggcctgttt ggctctaaaa gaagaaggct ataaggtcat acttgttaat
    19321 tcaaatcctg caacgattat gacagataaa gaaatcgctg ataaggtata tattgagcct
    19381 ttgactcttg aatttgttaa tcgtatcatt cgaaaagaaa ggccagatgc catactgcca
    19441 accttaggtg gtcaaacggg cctcaatatg gcaatggcat tatctaaagc tggtattctt
    19501 gatgatttag agattgaatt gttaggtact aagttatctg cgattgatca ggcagaagac
    19561 agagaccttt tcaaacaatt aatgcaagaa ctagaccaac ccattccaga atctacaatt
    19621 gttaaaacag ttgatgaagc tgttacattt gctagagata tcggttatcc agtcattgtg
    19681 cgcccagctt ttactcttgg tggaactggt ggaggaatat gtagtagtga agaagaactt
    19741 tgtgaaatta ctgaaaacgg cctaaagtta tcaccggtca cacagtgttt gattgaacgc
    19801 tcaattgctg gcttcaaaga aattgaatac gaagtaatgc gagatagtgc tgataacgcg
    19861 ctggttgttt gtaatatgga aaactttgat cccgtaggga ttcacacagg agattctatt
    19921 gtatttgcac caacacagac cctttctgat attgaaaatc aaatgttacg tgatgctagt
    19981 ttaaaaataa ttcgagctct gaaaattgaa ggtggctgta acgtccagtt agcactagat
    20041 ccttatagtt tcaaatatta tgttattgaa gtaaatccta gagtgtctcg ttcctctgcc
    20101 cttgcttcaa aagcaacagg ttatcccatt gccaaattgg ctgcaaagat tgctgttggc
    20161 ctaacacttg atgaaatgat taaccctatc acaggcacta cttacgctat gtttgaacct
    20221 acccttgatt acgttgttgc taaaattccg cgttttccgt ttgataaatt tgaacatggt
    20281 gaacgccaac tgggaactca aatgaaagca acaggtgaag tcatggctat tggccgtaat
    20341 ttggaagagt ccttactaaa agcctgtcgt tctttagaaa ttggtgtttg ccataatgag
    20401 atgacaagtt taagtaacat tagtgatgaa gagttagtga ctaaagtgat taaagctcaa
    20461 gatgatcgac ttttttactt gtcagaagca atacgacgag gttatagtat cgaagaactt
    20521 gagagcttga ccaagattga tttatttttc ttggataaat tacttcatat tgttgaaata
    20581 gagcaagatc tacaaatgca tgttgagcat ttggaaagcc tcaaaaaagc aaagcgttat
    20641 ggattttcag atcaaaaaat tgcagaaatt tggcaaaagg atgaaagtga tatcagagcg
    20701 atgagacatt ctcatagcct gtatcctgtc tataaaatgg tagatacttg tgcagctgaa
    20761 tttgatgcta aaacccctta tttttattca acatatgagt tggaaaatga atctgttcag
    20821 tcgaataaag aatcaatttt agttttaggt tcaggcccta ttcgtattgg tcaaggggtt
    20881 gaatttgact acgcaacggt tcattctgtt aaagccatcc aaaaagctgg ctatgaagcc
    20941 attatcatga attcaaatcc ggagactgta tctacagatt tttcggtatc agataaatta
    21001 tattttgaac cgttaacctt tgaagacgtc atgaatgtga tagatttaga gcagcctaag
    21061 ggagtaatag ttcagtttgg aggacaaact gctattaact tagcacaagc actttctgag
    21121 gcgggtgtta caatattagg aacacaagta gaggatttgg atagggctga agacagagat
    21181 ttatttgaaa aggcgttgaa agagcttgga atcccacaac cacaaggtca aacggcaact
    21241 aatgaagaag aagcattaga ggcagctaaa aaaattggtt ttccagtctt agttagacct
    21301 tcttacgtac ttggcggccg ggctatggaa attgttgaaa acaaagaaga tcttagagaa
    21361 tatataagaa cagcggttaa agcaagtcct gaacatccta tcttagtgga ctcttatatt
    21421 ttcggtaagg agtgtgaagt ggatgccatt tcagatggaa aatcagtctt aattcctggt
    21481 attatggagc acatcgaaag agcaggtgtt cattcaggag attcaatggc agtttatccg
    21541 ccacagcaat tatctaagca aattcaagaa acaatagctg agtatactaa acgtttagcc
    21601 attgggttaa attgtattgg tatgatgaat gttcaatttg ttattaaaaa tgaacaagtc
    21661 tatgttattg aagtgaatcc acgcgctagt cgtaccgtgc ctttcttatc aaaagtaaca
    21721 ggtattccga tggctcagat tgctactaag ttgattttag gtcaaacatt aaaggatcta
    21781 ggatatgaag atggtcttta tccccaaagt caactggtgc atatcaaagc gcctgtgttt
    21841 tcatttacaa agttggcaca ggttgatagt cttttaggtc ctgaaatgaa atcaactgga
    21901 gaagtaatgg gttcggatac tagtcttgaa aaagcacttt ataaagcgtt tgaagccaat
    21961 aattctcatt tatcagaatt tggtcaaatt gtctttacta tagcagatga tagtaaggca
    22021 gaagctctaa gtctagcaag acgatttaag gcgattggct atcaaattat ggctactcaa
    22081 ggtacagcag cttactttgc tgagcaaggc ttaagcgcgt gtttagttgg aaaaattggt
    22141 gatgctgcca atgacatccc gacactcgtg cgacatggtc acgttcaggc aattgttaat
    22201 acagttggaa ttaaacgcac agctgataaa gacggacaga tgattagaag ttcagctatt
    22261 gaacaaggag ttcctttatt tacagcttta gatactgcaa aagctatgtt aacggtttta
    22321 gaaagcagat gttttaacat cg
//
LOCUS       DATXIG010000022        21548 bp    DNA     linear   BCT 28-DEC-2023
DEFINITION  TPA_asm: Streptococcus pyogenes strain MGAS11113
            SAMN08555799-rid19682583.denovo.022, whole genome shotgun sequence.
ACCESSION   DATXIG010000022 DATXIG010000000
VERSION     DATXIG010000022.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN08555799
            Sequence Read Archive: SRR6740495
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pyogenes
  ORGANISM  Streptococcus pyogenes
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 21548)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 21548)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-OCT-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-OCT-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001918058.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001918058.2
            Genome Coverage       :: 368x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/12/2023 11:23:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 1,806
            CDSs (total)                      :: 1,769
            Genes (coding)                    :: 1,722
            CDSs (with protein)               :: 1,722
            Genes (RNA)                       :: 37
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1, 1 (5S, 23S)
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 47
            CDSs (without protein)            :: 47
            Pseudo Genes (ambiguous residues) :: 0 of 47
            Pseudo Genes (frameshifted)       :: 30 of 47
            Pseudo Genes (incomplete)         :: 20 of 47
            Pseudo Genes (internal stop)      :: 9 of 47
            Pseudo Genes (multiple problems)  :: 11 of 47
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..21548
                     /organism="Streptococcus pyogenes"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN08555799-rid19682583.denovo.022"
                     /strain="MGAS11113"
                     /serotype="Emm28"
                     /isolation_source="normally sterile site"
                     /host="Homo sapiens"
                     /db_xref="taxon:1314"
                     /country="Finland"
                     /collection_date="2002"
                     /collected_by="National Public Health Laboratory"
     gene            79..333
                     /locus_tag="VC122_000920"
     CDS             79..333
                     /locus_tag="VC122_000920"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008087830.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1912 family protein"
                     /protein_id="HEP5640856.1"
                     /translation="MSYEKEFLKDFEDWVKTQIQVNQLAMATSQEVAQEDGDERAKDA
                     FIRYESKLDAYEFLLGKFDNYKNGKAFHDIPDELFGARHY"
     gene            354..749
                     /locus_tag="VC122_000921"
     CDS             354..749
                     /locus_tag="VC122_000921"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014635594.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-hairpin-helix domain-containing protein"
                     /protein_id="HEP5640857.1"
                     /translation="MAKQKNRKKALKLEMKRNGLLKKAGQVFDKAVESVETAVDKTIS
                     AGKNLVEKGSQTVENLTASKERLTLDEFAGQPVVAGIRSDLVETLYAEGIHSAQAFKE
                     WTEKDLLALKGIGPATVKKLVENGASFKK"
     gene            1035..1354
                     /locus_tag="VC122_000922"
                     /pseudo
     CDS             1035..1354
                     /locus_tag="VC122_000922"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002351072.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
     gene            1351..1914
                     /locus_tag="VC122_000923"
     CDS             1351..1914
                     /locus_tag="VC122_000923"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002983757.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="shikimate kinase"
                     /protein_id="HEP5640858.1"
                     /translation="MNLIIIGAQASGKMTIEQEVARQTGMNLFHNHDSIDFVLRFMPW
                     SQESTALIERMRFAFFETFAKTGQDMIFTIVIDFNDPNDVAMLEKIQAVFQSYDQEVL
                     FVELKTDIEERLKRNRTENRLKHKPLKRNIEWSEQDIQSTMAYAVFNPEEPPKTLTHY
                     QKINNTHLTAAETAQLIIQKMTHIKEN"
     gene            1916..4564
                     /locus_tag="VC122_000924"
     CDS             1916..4564
                     /locus_tag="VC122_000924"
                     /EC_number="6.1.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011284987.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="valine--tRNA ligase"
                     /protein_id="HEP5640859.1"
                     /translation="MTELSPKYNPAEVEAGRYQKWLDADVFKPSGDQKAKPYSIVIPP
                     PNVTGKLHLGHAWDTTLQDIIIRQKRMQGFDTLWLPGMDHAGIATQAKVEERLREQGI
                     SRYDLGRDKFLDKVWEWKDEYATTIKEQWGKMGLSVDYSRERFTLDEGLSKAVRKVFV
                     DLYKKGWIYRGEFIINWDPAARTALSDIEVIHKDVEGAFYHMNYMLEDGSRALQVATT
                     RPETMFGDVAVAVNPEDPRYKDLIGKNVILPIVNKLIPIVGDEHADPEFGTGVVKITP
                     AHDPNDFEVGQRHNLPQVNVMNDDGTMNELAGDFAGMDRFEARQATVAKLEELGALVN
                     IEKRVHSVGHSERSGAVVEPRLSTQWFVKMDELAKQAMDNQETDNRVDFYPPRFNDTF
                     LQWMENVHDWVISRQLWWGHQIPAWYNAEGEIYVGEEAPEGDGWTQDEDVLDTWFSSA
                     LWPFSTMGWPDTDVEDFKRYFPTSTLVTGYDIIFFWVSRMIFQSLEFTGRQPFQNVLI
                     HGLIRDEEGRKMSKSLGNGIDPMDVIEKYGADSLRWFLSNGSAPGQDVRFSYEKMDAS
                     WNFINKIWNISRYILMNNEGLTLEEAESNVAKVAASEAGNVTDQWILHNLNETIAKVT
                     ENFDKFEFGVAGHILYNFIWEEFANWYVELTKEVLYSDNEAEKVITRSVLLYTLDKIV
                     RLLHPIMPFVTEEIYAQYAQGSIVTVDYPVVRPAFENEAAHKGVERLKDLIRAVRNAR
                     AEVNVAPSKPITILVKTADSELEDFFTSNVNYIKRFTNPEKLEISSAIAAPELAMTSI
                     ITGAEIYLPLADLLNVEEELARLDKELAKWQKELDMVGKKLGNERFVANAKPEVIQKE
                     KDKQADYQAKYDATQERIAEMKKIKS"
     gene            4718..7126
                     /locus_tag="VC122_000925"
     CDS             4718..7126
                     /locus_tag="VC122_000925"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002989028.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CRISPR-associated helicase/endonuclease Cas3"
                     /protein_id="HEP5640860.1"
                     /translation="MRMILAHYDCKKDKKQSLDEHLWHVACSSRQEASIIGQGDVLFL
                     IGLYHDLGKADRTFQDKLLNNPNRHVDHSYAGAKYLCSIIGPHLKNRGVDKNERMTFN
                     EMVGYVISAHHGMYDLCYYFDDAEYYGFNKFKNRINRDLDGYHYHEDIKGYALKLEKK
                     LCDYGYKDLRELIDKAFDNYQQAMSSLNWQDKSEWDYYQSCMVRLYLSLLKNADILDT
                     VNAYGLKISPMDKTERSFLKHSYLAAIEQKYASFGQPNNQLNTIRTEIAERVKERGKR
                     DSKGIYRLDLPTGAGKTNLSMRYAFHQLVHHDKSRFFYITPFLSVLEQNASEIRKVTG
                     DLGVLEHHSNVVKQANEDDDDKDSLLSAYLSDSWDSQVVLTSMVQFFQTLFKTKSANL
                     RRFSSLINSVVILDEVQSLPIEVTTLFNLTMNFLNKVMDTTIVLCTATQPAYDSSEID
                     HRICYGGNLGELAEIVELTIEEKQIFSRTELRKFDDSDQKVHLTDVINLILGEENSVL
                     AIFNTKKTVHNCYTMLKDITDRPVYQLSTNMCAQHRLDLIAKIKTELQNNIPIICIST
                     QLIEAGVDVDFHRVIRSYSGIDSIVQAAGRCNREGKRDKGQVTLVNLTNEEENISRLT
                     EIKAKKEATESILHKIGSPIDISTLNRDFFEYYYANNQGLMDYPLEDNLSIYDYLSLN
                     IYQTANKKFKGKLKQAFKTAGAKMNLINNDMIGILVPYGEAEKKLAYLEELGMSHFLS
                     AEDYQTIKSLLKELQPFTVNVRENDPLFETTKSYLNGQILVLTSEYYDTERGVKYDSA
                     SFYF"
     gene            7259..7987
                     /gene="cas5c"
                     /locus_tag="VC122_000926"
     CDS             7259..7987
                     /gene="cas5c"
                     /locus_tag="VC122_000926"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011528831.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I-C CRISPR-associated protein Cas5"
                     /protein_id="HEP5640861.1"
                     /translation="MYRSRDFYVRVSGQRALFTNPATKGGSERSSYSVPTRQALNGIV
                     DAIYYKPTFTNIVTEVKVINQIQTELQGVRALLHDYSADLSYVSYLSDVVYLIKFHFV
                     WNEDRKDLNSDRLPAKHEAIMERSIRKGGRRDVFLGTRECLGLVDDISQEEYETTVSY
                     YNGVNIDLGIMFHSFAYPKDKKTPLKSYFTKTVMKNGVITFKAQSECDIVNTLSSYAF
                     KAPEEIKSVNDECMEYDAMEKGEN"
     gene            7987..9882
                     /gene="cas8c"
                     /locus_tag="VC122_000927"
     CDS             7987..9882
                     /gene="cas8c"
                     /locus_tag="VC122_000927"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017647433.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I-C CRISPR-associated protein Cas8c/Csd1"
                     /protein_id="HEP5640862.1"
                     /translation="MDFFTSLLKTYEKAELADLVDHQKRSNEPVLLPIYHTSLKSNGK
                     NIISVKLDKDGQFHKAEFMADKQMIIFPVTADSVARSGSHPAPHPLVDKFAYYSAEMG
                     QSQYDSFHKQLNNWIDYCEEGDVKRFLTIVQQFILKPEFLTLILDSLIGPDYQNNQLK
                     VTFCDATGKEKLIDLSACFLEFSIDQFQGFKNESVSTFKTLHQSYISFVEANREKLGI
                     CNISGREEQLTDKHRGLMGNAKIISVSNKREAYKGRFREREDVFSVGYETSEKIHLML
                     KYLLENKNTSTWLGSSQYLINWFSDDLTNDSRLDIVSPIFDDGLEEDDDDDTPPVITL
                     ATEDNKRIGKSFIKGQKLFANDATYYVAILNKTSNGRIALKYFRQLQASQLLTNLNKW
                     QETYSWESRSKFGKSRLRTPTFHDILNVSYGVDRDRFLELDNDNFKSDQIQKLVASLI
                     DGKPMPQSIVKKLGNNVKERHRYRKHWYQVEQVCLAILHKQNGEEFSPMLDHTNQNRS
                     YLFGRLLAIFELIETLRYGLDGNNNDRITNAERYWTAYTGQPTKLMMLLENKIKPYEE
                     PLKLNRRGSWMKLEKEKEEILELLNPLLETETMEKPLDYRFIFGYYAEKNYYYTKQNT
                     EVTESEE"
     gene            9887..10735
                     /gene="cas7c"
                     /locus_tag="VC122_000928"
     CDS             9887..10735
                     /gene="cas7c"
                     /locus_tag="VC122_000928"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010922510.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I-C CRISPR-associated protein Cas7/Csd2"
                     /protein_id="HEP5640863.1"
                     /translation="MLEHKIDFMVTLEVKEANANGDPLNGNMPRTDAKGYGVMSDVSI
                     KRKIRNRLQDMGKSIFVQANERIEDDFRSLERRFSQHFTAKTPDKEIEEKANALWFDV
                     RAFGQVFTYLKKSIGVRGPVSISMAKSLEPIVISSLQITRSTNGMEAKNNSGRSSDTM
                     GTKHFVDYGVYVLKGSINAYFAEKTGFSQEDAEAIKEVLVSLFENDASSARPEGSMRV
                     CEVFWFTHSSKLGNVSSARVFDLLEYHQSIEEKSTYDAYQIHLNQEKLAKYEAKGLTV
                     EILEGL"
     gene            10737..11411
                     /gene="cas4"
                     /locus_tag="VC122_000929"
     CDS             10737..11411
                     /gene="cas4"
                     /locus_tag="VC122_000929"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002989039.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CRISPR-associated protein Cas4"
                     /protein_id="HEP5640864.1"
                     /translation="MVYAEDDYLMLSGIQHFQFCKRQWALIHIEQQWLDNEATAHGQV
                     LHTKADNPYIKEKRKELLVSRAMPISSAELGLSGIMDVVEFYKDDQGVSLRGKRGKWL
                     PKVVEYKRGKPKKDTRDIVQLVAQTMCLEETLDCDINEGCLYYHSVNQRVIVPMTSAL
                     RQEVKELAAEMQEVYQSQMLPKAAYFKNCQLCSLVDICKPRLSKKTRSVSRYINEAMT
                     SEEMDL"
     gene            11408..12433
                     /gene="cas1c"
                     /locus_tag="VC122_000930"
     CDS             11408..12433
                     /gene="cas1c"
                     /locus_tag="VC122_000930"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015057908.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I-C CRISPR-associated endonuclease Cas1"
                     /protein_id="HEP5640865.1"
                     /translation="MKKLLNTLYLTQEDFYVTKEGDNIVIKQEGKVLKRFPFRIIDGI
                     VCFSYLGVSPALVKLCTENQINLSFHTPQGRFCGRYIGSTNGNVLLRREHYRLSDREE
                     SLEYAKRFILAKISNSRKYLLRFKRDHRQQIDTKLFEAVNDELIWALEMVQAADNKDS
                     LRGIEGQAANQYFRIFNDLVLTDKKTFYFQGRSKRPPLDCVNALLSFGYSLLTFECQS
                     ALEAVGLDSYVGFFHTDRPGCASLALDLVEEFRSYIVDRFVFSLINKGQLQKKHFEVK
                     ENGSILMTENGRAIFIDLWQKRKHTEVEHPFTKEKVKLMLLPYVQAQLLAKAIRGDLE
                     SYPPFMV"
     gene            12444..12737
                     /gene="cas2"
                     /locus_tag="VC122_000931"
     CDS             12444..12737
                     /gene="cas2"
                     /locus_tag="VC122_000931"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002280569.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CRISPR-associated endonuclease Cas2"
                     /protein_id="HEP5640866.1"
                     /translation="MMVLVTYDVNTETPAGRKRLRHVAKLCVDYGQRVQNSVFECSVT
                     PAEFVDIKHRLTQIIDEKTDSIRFYLLGKNWQRRVETLGRSDSYDPDKGVLLL"
     gene            13143..13853
                     /gene="ccdA2"
                     /locus_tag="VC122_000932"
     CDS             13143..13853
                     /gene="ccdA2"
                     /locus_tag="VC122_000932"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011018020.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiol-disulfide oxidoreductase-associated
                     membrane protein CcdA2"
                     /protein_id="HEP5640867.1"
                     /translation="MTLSLVLMVSVFGAGLLSFFSPCIFPVLPVYLGILLDADDSKTI
                     TIFGKKLYWYGIVKTLAFIFGLSTIFVILGYGAGFLGNILYAVWFRYLLGALVIILGI
                     HQMGLITIKSLQFQKSLTFHNNKNRNGLFNAFILGLTFSFGWTPCVGPVLSSVLALVA
                     SGGNGAWQGGVLMIIYTLGLGIPFLLISFASGIVLKQFNKLKPHIFLLKKVGGVLIIV
                     MGILLMTGTLNNLAQLFG"
     gene            13866..14489
                     /locus_tag="VC122_000933"
     CDS             13866..14489
                     /locus_tag="VC122_000933"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015017249.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TlpA family protein disulfide reductase"
                     /protein_id="HEP5640868.1"
                     /translation="MKKGLLVTTGLACLGLLTACSTQDNMVKKEITQDKMSMAVKKKD
                     KMSTSKDKSMMADKSSDKKMTNDGPMAPDFELKGIDGKTYRLSEFKGKKVYLKFWASW
                     CSICLSTLADTEDLAKMSDKDYVVLTVVSPGHQGEKSEADFTKWFQGTDYKDLPVLLD
                     PDGKLLEAYGVRSYPTEVFIGSDGVLAKKHIGYAKKSDIKKTLKGIH"
     gene            14532..15638
                     /gene="msrB"
                     /locus_tag="VC122_000934"
     CDS             14532..15638
                     /gene="msrB"
                     /locus_tag="VC122_000934"
                     /EC_number="1.8.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012560832.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide-methionine (R)-S-oxide reductase MsrB"
                     /protein_id="HEP5640869.1"
                     /translation="MEHKWKLWLFIVGVVLLIGGASVLIQKGQAKAFSQGKPQLPAKK
                     VKSTKVDPNAPVDQKVIYLAGGCFWGVEEYFSQVDGVLDAVSGYANGRGDTTNYQLIH
                     QTGHAETVEVTYDANRISLKELLLHFFRIIDPTSLNKQGNDRGSQYRTGIYYTDKADL
                     AIIDEVFKEKAKDYKKKIVVEKAPLKHFIKAEDYHQDYLKKNPNGYCHIDINQATYPV
                     IDESKYPKPSATEIKAKLSADEYRVTQKNETEKAFSNRYWDSFDAGIYVDVVTGEPLF
                     SSKDKFESGCGWPSFSRPISPDVVRYKEDKSFNMTRTEVRSRSGNSHLGHVFTDGPKD
                     QGGLRYCINSLSITFIPKADMEAKGYGYLLSSVE"
     gene            15726..16466
                     /locus_tag="VC122_000935"
     CDS             15726..16466
                     /locus_tag="VC122_000935"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011018017.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HEP5640870.1"
                     /translation="MYSLLIVEDEYLVRQGIRSLVDFSQFKIDRVNEAENGQLAWDLF
                     QKEPYDIVLTDINMPKLNGIQLAELIKQESPQTHLVFLTGYDDFNYALSALKLGADDY
                     LLKPFSKADVEDMLGKLQQKLDLSKKTETIQELVEQPQKEVSAIAMAIHERLADSDLT
                     LKSLAQQLGFSPNYLSVLIKKDLGMPFQDYLVQERLKKAKLLLLTSNLKIYEIAEQVG
                     FEDMNYFSQRFKQLVGVTPSQYKKGGQA"
     gene            16463..18196
                     /locus_tag="VC122_000936"
     CDS             16463..18196
                     /locus_tag="VC122_000936"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012767315.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sensor histidine kinase"
                     /protein_id="HEP5640871.1"
                     /translation="MKRYPLLVQLISYVFVIVIALITTLGLLYYQTSSRNIRQLIERD
                     TRQSIRQSSQFIDAYIKPLKETTSVLAKNTEIQAFASQIHQENDKQVLQLMKMVLATN
                     SDLQAAVLVTKDGRTVSTNSQLTMKTSSDMMAEPWYKAAIDRQAMPVLTPARQLSLSS
                     KKEWVVSVTQEVVDRAGHNLGVLRLDIAYPTIKASLDQLQLGRQGFAFIVNDKHEFVY
                     HPKKSVYSSSKEMAAMKPYLAIQNGYTKDKTSFVYQKLIPNSQWTLVGVASLDQLHRV
                     QRQIFWSFLGTGLLACLICGFATVLALRRWIRPIQQLQQVILAIQKGDRQLRAQETGS
                     PELTNLAQQFNALLDQIDSLMVAVADKEKAIGQYRLQALASQINPHFLYNTLDTIIWM
                     AEFNDSKRVVEVTKSLAKYFRLALNQGNEYIRLADELDHVSQYLFIQKQRYGDKLSYE
                     VQGLDVYADFVIPKLILQPLVENAIYHGIKEVDRKGMIKVTVSDTAQHLVLTVWDNGK
                     GIEDSSLTNSQSLLARGGVGLKNVDQRLKLHYGEGYHMTIHSQSDQFTEIQLTLPKMH
                     ELMADDTQENE"
     gene            18269..19348
                     /locus_tag="VC122_000937"
     CDS             18269..19348
                     /locus_tag="VC122_000937"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012767314.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MmcQ/YjbR family DNA-binding protein"
                     /protein_id="HEP5640872.1"
                     /translation="MSLASDYFSRQTPIVEKLMAYGFEKRNNGYFYNERFMEEEFEAQ
                     LRIDEAGNIWDRVIDCDLEEDYLPLQQAAWQGTYTGQVRAAYLELLERLSVACFEVTP
                     FQSMQANRLAKHITKEWSDPMDYPFEKHPDLATYRVEGKWYAMIFSLLADKLDQIPER
                     LVGQTCEVMTVKVNPKAFPQLLQQEGIYPAYHMSKKNWISIILDDKVTDDKLWTLVTQ
                     SRQLVNPNGLSNPNGPDYWVIPANLKYYDIDAEFAANDVILWTQKAGIAVGDYVLIYI
                     TAPVKSIRYVCQVLETNIPNEGYRKNPNIDKLMRLRKRQQYKDGLLSFDLMKKHGVAA
                     VRGPRRLSPQLIAFLKEKEYFKENN"
     gene            19362..20078
                     /locus_tag="VC122_000938"
     CDS             19362..20078
                     /locus_tag="VC122_000938"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014407705.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="B3/4 domain-containing protein"
                     /protein_id="HEP5640873.1"
                     /translation="MVEFVADQSFWELFPDAKLGVVLLKDVQNQAESPEDVKQLLADS
                     HELAKAYLTADNFSDNQVVQVYRKAYQHFKTKKGARSSIEALLKRVSNGQSIPSINPL
                     VDIYNAASLRFGLPVGAEDSDSFIGDLRLTITDGGDDFYLIGDADNNPTLPNELCYKD
                     DIGAVCRCLNWRDGERTMVTEHTKNAFLIIEALDQEGQNRLQEALDFIEGSAKMYLHA
                     ITSVHVLDKDNPHVPLFTEN"
     gene            complement(20244..20717)
                     /locus_tag="VC122_000939"
     CDS             complement(20244..20717)
                     /locus_tag="VC122_000939"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008087919.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arginine repressor"
                     /protein_id="HEP5640874.1"
                     /translation="MNKKETRHQLIRSLISETTIHTQQELQERLQKNGITITQATLSR
                     DMKELNLVKVTSGNDTHYEALAISQTRWEHRLRFYMEDALVMLKIVQHQIILKTLPGL
                     AQSFGSILDAMQIPEIVATICGDDTCLIVCEDNEQAKACYETLSHYTPPFFFSNK"
     gene            20859..21539
                     /locus_tag="VC122_000940"
     CDS             20859..21539
                     /locus_tag="VC122_000940"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003051925.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Crp/Fnr family transcriptional regulator"
                     /protein_id="HEP5640875.1"
                     /translation="MIRREDYQYLRKLNDFRYFSIEQFDKIVGQMEFRKAKKDHILFF
                     EGDKRDKLFLVTSGYFKVEQSDQSGTFMYTDFIRHGTIFPYGGLFTDDYYHFSVVAMT
                     DVTYFYFPVDLFEDYSLENRLQMKHLYSKMSKLLELHELRVRNLITSSASSRVIQSLA
                     ILLVEMGKDSDTLPFQLTTTDIAQISGTTRETVSHVLRDLKKQELITIKGKYLTYLDK
                     DYFLQYTK"
BASE COUNT         6772 a         3843 c         4677 g         6256 t
ORIGIN      
        1 cagttattaa taacatggca caagaggcct gccttatggt ataatggtaa agatgatttc
       61 agaaagagga agctaagcat gtcttatgaa aaagaatttt taaaagattt tgaagactgg
      121 gttaaaactc agattcaagt caatcagcta gccatggcaa ccagtcaaga agttgcccaa
      181 gaagatggcg atgaaagggc taaagatgcc tttattcgtt atgaaagtaa gttagacgcc
      241 tacgaatttt tactaggtaa atttgacaat tataaaaatg gtaaagcctt tcacgatatt
      301 ccagatgagt tatttggagc aaggcattat taaggacaag gagacacgaa atagtggcaa
      361 aacaaaaaaa tcgtaaaaaa gcattgaagt tagaaatgaa gcgtaatggc ttgttaaaaa
      421 aagcaggtca agtttttgat aaggcagttg aaagtgttga aacagccgtg gataaaacca
      481 tttctgctgg taaaaatctt gtggaaaaag gcagccaaac ggttgaaaac ctgacagcaa
      541 gcaaggaaag acttactctt gatgagtttg cagggcagcc agttgtagct ggtatccgct
      601 ctgaccttgt cgaaacccta tatgcagaag gcatccattc agcgcaagct tttaaggagt
      661 ggacagaaaa agacctcctt gctcttaagg gcattggccc agcaacggtt aaaaaattgg
      721 tcgaaaatgg cgcaagcttt aaaaaatagt catcttctcg ttgcttaatg gctataaatt
      781 tgttagaata ttagcaatca gaggtgcatt gagttggtct gttacagaga gcggtggtgt
      841 tgagaaatcc gcacagatgt caaggctggt agctgaggat aagaaatcag atatgaaaag
      901 tagcagatcc attttatggt ctgaaacagt ggtggtaccg cggaaatcgt aacacgaaat
      961 tcgtccctgt ccagtgtaag ctggataggg attatttggt ttcatttgaa agaagatgat
     1021 ggcttattgt taaaatgttg ttagaagtat acaatagaaa aactttgccc aaagtggaaa
     1081 cagaacggct gctattacgt caacgaaaag gggacaaggc attattccag aagcggcttc
     1141 agctctgatt gaaattggtt ttaccttact tgatttgcat aaaattgagc tgggttgcta
     1201 cgactacaac aagcaaagtc aggcagtggc tcgcaaatta ggctttacct tagaagccaa
     1261 tgtccgtgag cgtaaggacg ttcaaggtcg ccgctgtggc ggcatgcgtt ttgggttact
     1321 gagaagtgag tgggaggagc agaaacaaaa atgaacctta tcatcattgg tgctcaagcg
     1381 tcgggcaaga tgaccattga gcaagaagtt gccaggcaga caggcatgaa tttgtttcat
     1441 aatcacgatt ctattgattt tgttttacgg tttatgcctt ggtctcaaga gagcactgcc
     1501 ttgattgaac gtatgcgttt tgcttttttt gaaacctttg ctaagacagg tcaagacatg
     1561 atttttacta ttgtaattga cttcaatgat ccaaatgatg tagcaatgct tgaaaagatt
     1621 caggctgttt ttcagtctta tgaccaagag gttctctttg tcgaattaaa aacagatata
     1681 gaggaacgtc ttaaacgcaa tcgcacagaa aatcgtctca aacataaacc tcttaaacgc
     1741 aacatcgagt ggtcagaaca agatattcaa tccacgatgg catatgctgt ttttaatcca
     1801 gaagaaccac ccaaaacgct cacgcattac caaaaaatca ataataccca tctcaccgct
     1861 gctgaaacag cgcagcttat tatccaaaaa atgactcata tcaaggagaa ctaagatgac
     1921 agaactatca cctaaataca atcctgccga ggttgaggca ggtcgctatc aaaaatggct
     1981 tgatgcagat gtttttaagc catcaggaga ccaaaaagca aaaccgtatt ctatcgtgat
     2041 tccaccgcca aatgtgactg gtaagttgca cttaggacac gcctgggata cgacgcttca
     2101 agatattatc attcgtcaaa aacgcatgca gggctttgat acgctttggc ttccagggat
     2161 ggaccatgca ggaattgcga cacaggctaa ggtagaggag cgtctgcgcg agcaggggat
     2221 ttctcgttac gaccttggtc gtgacaaatt cctagacaag gtctgggagt ggaaagacga
     2281 atatgccact acgattaaag agcagtgggg caaaatgggg ctttctgtgg actacagccg
     2341 tgagcgcttc acccttgatg agggcttgtc aaaagcagtt cgcaaggttt ttgttgacct
     2401 ctacaaaaaa ggctggattt accgtggtga attcatcatc aactgggatc ctgctgccag
     2461 aactgccctt tctgacatcg aggttatcca caaggatgtt gagggagctt tctatcacat
     2521 gaactatatg ttggaagatg gctcacgcgc ccttcaagtg gcaacaacgc gaccagaaac
     2581 catgtttgga gacgttgccg ttgccgtaaa cccagaagat cctcgttaca aagatttgat
     2641 tggcaaaaat gttattttac caatcgtgaa taaattgatt ccaatcgtgg gtgacgagca
     2701 tgctgaccct gaatttggga ctggagttgt gaaaatcacg cctgcccatg atcctaacga
     2761 tttcgaggtt ggtcaacgcc ataatctacc acaagtgaat gtcatgaacg atgacggtac
     2821 catgaatgag ttagctggcg actttgcagg catggaccgt tttgaagctc gtcaagctac
     2881 cgttgctaaa cttgaagaac ttggtgccct agtaaacatc gaaaaacgtg ttcactcagt
     2941 aggtcactca gaacgttctg gtgcagtcgt tgagcctcgt ctatccaccc aatggtttgt
     3001 aaaaatggat gagcttgcta aacaagctat ggacaaccaa gaaacagata accgagtaga
     3061 tttctaccca ccacgcttta atgatacctt ccttcaatgg atggaaaatg tccatgactg
     3121 ggtaatctcg cgtcagcttt ggtgggggca ccaaattcct gcttggtaca atgcagaagg
     3181 tgagatttac gttggcgaag aagcaccaga aggtgatggc tggactcaag atgaagatgt
     3241 tcttgacact tggttctcat ctgctttatg gccgttctca actatggggt ggcctgacac
     3301 agatgtagaa gatttcaaac gttatttccc aacctcaacc ttagtaacag gttatgacat
     3361 cattttcttc tgggtgtcac gtatgatttt ccaatcgctt gaatttaccg ggcgtcagcc
     3421 attccaaaat gtcctgattc acggtcttat tcgtgacgaa gaaggacgca aaatgtctaa
     3481 atcattgggg aacgggattg atccgatgga tgtcattgag aaatatggtg ccgatagctt
     3541 acgttggttc ctctcaaacg gctctgcccc aggacaagac gtgcgtttct catacgaaaa
     3601 aatggacgcc tcttggaatt tcattaataa aatttggaat atttctcgct atatcctcat
     3661 gaataatgaa gggttgacct tggaagaagc tgaaagtaat gtggctaagg tagctgcctc
     3721 agaagctgga aatgtgactg accaatggat tctccacaat ctcaatgaaa ccatcgctaa
     3781 agtgacagaa aactttgaca agtttgagtt tggtgtggca ggacacattc tttacaactt
     3841 catctgggaa gaatttgcca actggtatgt ggaattgacc aaggaagtgc tatatagcga
     3901 caacgaagct gaaaaagtta tcacgcgctc tgtccttctt tatactttgg acaagattgt
     3961 acgtctcctt cacccaatca tgccatttgt aacagaagaa atttacgctc aatatgccca
     4021 aggttctatt gtgacggtgg actatcctgt ggttcgccca gcttttgaaa atgaagctgc
     4081 gcataaaggt gtggaaaggt tgaaagacct gattcgtgca gttcgtaatg cgcgcgctga
     4141 agttaacgtg gcaccatcaa aaccaatcac cattttggtg aagacagcag atagtgagtt
     4201 ggaagacttc ttcaccagca acgtgaacta catcaaacgt ttcaccaatc ctgaaaaatt
     4261 agaaatcagc tcagccattg ctgcgccaga gctggctatg accagtatca tcacaggtgc
     4321 tgaaatctac ttgccactag cagatttgct taacgtggaa gaagaactcg ctcgcctaga
     4381 taaagaactg gctaaatggc aaaaagaatt ggacatggtc ggcaaaaaac tcggaaacga
     4441 acgctttgta gccaacgcca aaccagaagt catccaaaaa gaaaaagaca aacaagccga
     4501 ctaccaagcc aaatacgacg caacccaaga acggattgca gagatgaaga aaattaaatc
     4561 atagtgttta gaacgcagtt gaaaaactgt gttcttttct tactaataga gatattagat
     4621 tgaagtttaa ttatgagaaa aacgctttct ttatttaaag agttatgtta caatactatt
     4681 acaatcatcg tacaaatagt gttattttga ggggcatatg agaatgattt tagcacacta
     4741 tgactgtaaa aaagataaaa agcaatcttt agatgagcat ttatggcatg tggcctgttc
     4801 tagtcgacag gaagcatcta taattggtca aggagatgtg ctttttttaa ttggtcttta
     4861 ccacgacctg ggcaaagctg atcgaacctt tcaagataaa ttattaaata atccaaatcg
     4921 gcatgttgat cactcttatg caggggcaaa atacttatgt tctattattg ggcctcatct
     4981 aaaaaaccga ggggttgata aaaatgagag aatgacattc aacgaaatgg tggggtatgt
     5041 catctctgct catcatggga tgtatgattt atgctactat tttgacgatg ctgaatatta
     5101 tggctttaat aagtttaaaa atcgtatcaa tagagactta gatggttatc actatcatga
     5161 agatattaaa gggtacgctc taaaattaga aaaaaaatta tgtgattatg gctacaaaga
     5221 tttaagggag cttattgata aagcttttga taattaccaa caagccatgt cttccttaaa
     5281 ctggcaagat aagagtgagt gggattatta tcagtcttgt atggtgagac tttacttgtc
     5341 actcttaaaa aacgctgata ttttggacac agtaaatgcc tatggcctta agataagtcc
     5401 tatggataaa acagagcgat cctttctaaa acactcctat ttagcggcca ttgaacaaaa
     5461 atatgctagc tttggacagc caaacaatca gttgaacact attcggacag aaatcgctga
     5521 gcgtgttaaa gaaagaggta aacgagattc caaggggatt tatcgcttag atttaccgac
     5581 aggagctggc aagactaatc ttagtatgcg ttatgcgttt caccaattag ttcatcacga
     5641 caaatcaagg tttttttaca taacgccctt tctttcggtt cttgaacaaa atgcttccga
     5701 aattagaaaa gttacaggtg accttggcgt cctagaacac cattccaatg tggtgaaaca
     5761 ggctaatgaa gatgatgatg acaaggacag tttattgtca gcttatctta gtgatagctg
     5821 ggacagtcaa gtagtcttga cttctatggt tcaatttttc caaacacttt tcaaaacaaa
     5881 atcagctaat ctgagacgtt tttcaagttt gattaatagt gttgtgattc tagatgaagt
     5941 tcaatccctg cctattgaag tcaccacttt gtttaattta acgatgaatt ttttaaataa
     6001 agttatggat acaaccatcg ttctttgcac agcaacacaa cctgcttatg attcttcaga
     6061 gattgaccat cgtatctgtt atggagggaa cttgggagaa ttagctgaaa tcgttgagtt
     6121 aacgattgaa gaaaaacaga ttttttcaag gacagagctt agaaaatttg atgatagtga
     6181 tcagaaagtt cacttgactg atgttattaa tcttattcta ggtgaggaaa actcagttct
     6241 tgctattttt aatacgaaaa aaacggttca taactgctat actatgctaa aagacattac
     6301 tgatagaccg gtctatcagc tttcgacaaa tatgtgtgcg cagcatagac ttgacttgat
     6361 tgctaagatc aaaacggagt tacaaaataa tatccctatt atttgtatta gcacgcaatt
     6421 aattgaagca ggtgtagatg ttgattttca tcgcgtcatt cgttcctact cagggattga
     6481 ttctattgtt caggctgctg gacggtgtaa ccgagaaggc aaacgagata aagggcaagt
     6541 cactcttgtc aatctgacca atgaagagga aaatatttct aggctgacag aaataaaagc
     6601 taaaaaagaa gccacagaat ctattcttca taagattggg tctccaattg atatctcaac
     6661 tttaaaccgt gacttttttg agtattatta tgccaataat cagggactga tggattatcc
     6721 tttggaagac aacctatcaa tctacgacta tttaagcctt aatatttatc agacggcaaa
     6781 taaaaagttc aaaggtaagt taaaacaagc ttttaaaaca gcaggagcca aaatgaacct
     6841 catcaataat gatatgatag gaattctcgt accttatggc gaagctgaga aaaaattggc
     6901 ttatttagaa gaattgggta tgtctcactt tttatcagca gaagattatc aaacgataaa
     6961 atcattacta aaagagttac aaccttttac ggttaatgtc cgcgagaacg atcctctctt
     7021 tgagacaaca aaatcttatc taaatggtca gattctggtt ttgacgtcgg agtattatga
     7081 cacggaaaga ggagttaaat acgattcagc tagcttttac ttctaactca aaacgaaagc
     7141 agattaacaa aaggttgtta gaggaccttg ttaacctgcc aatcatcatt aataattatt
     7201 atcaatttag actatttaat aaaattagat tacaaaaaaa cagaaggagg aaagtagctt
     7261 gtacagatct agagacttct acgtgagagt aagtggtcag cgagctcttt ttacaaatcc
     7321 agccacaaaa gggggatcgg aacgctcatc ctattcggtt ccgactagac aggcactgaa
     7381 tggtatcgtt gatgccatct attataagcc gacctttact aatatcgtca cagaggttaa
     7441 ggttattaac cagattcaaa ccgaattaca gggtgtcagg gctctgttac atgattatag
     7501 tgcagattta agttatgtat cctatttgag tgatgttgtt tatctgatca agtttcattt
     7561 tgtttggaat gaagatagaa aagatttgaa ctcagataga cttccagcta aacatgaagc
     7621 cattatggag cgttctattc gtaaaggggg acgtcgagat gtgtttttgg gtacaagaga
     7681 atgtttaggg cttgtagatg atatcagcca agaagagtat gagactactg tgtcgtatta
     7741 taatggtgtc aatatcgact tgggaatcat gttccattcc tttgcctatc cgaaggacaa
     7801 aaagacacca ttaaaatcat actttacaaa gactgtgatg aaaaatggag tcattacgtt
     7861 taaagcacag tctgaatgcg atattgttaa cacgctttct agttatgctt ttaaagcacc
     7921 agaggagata aaatcggtta acgatgaatg catggagtat gatgccatgg agaaaggaga
     7981 aaactgatgg atttttttac ttctctcttg aagacttatg aaaaagcaga gctagcagac
     8041 ttggttgatc accaaaaaag aagtaatgag ccggttttac tgccgattta tcatacgagt
     8101 ttaaagtcaa atggcaaaaa tatcatttca gtgaaacttg acaaagatgg ccagtttcac
     8161 aaggcagaat ttatggcaga taagcaaatg attatttttc ctgtaacggc tgattctgtt
     8221 gctaggtcag gtagtcatcc tgcaccgcat cccctagtcg ataaatttgc ttattatagt
     8281 gctgaaatgg ggcagtctca gtatgattct ttccataagc aacttaataa ctggattgat
     8341 tattgtgagg agggtgacgt caagaggttt ttaaccattg ttcagcaatt tattttgaaa
     8401 ccagaatttc tgacactgat tctcgattct ttaattggtc ctgattatca aaataatcaa
     8461 ttaaaagtca cattttgtga tgccactgga aaagaaaaat taattgattt atcagcttgc
     8521 tttttagaat tttcaattga tcagttccag ggctttaaaa atgaatcggt ttcgacattt
     8581 aaaactttac accaatccta tatttctttt gttgaagcca atcgtgaaaa gcttggtatt
     8641 tgtaatatta gtggacgaga ggaacagctt accgataagc atagaggttt gatggggaat
     8701 gctaaaatca tctctgttag taataaaaga gaagcttata aaggacgttt tagagaacgt
     8761 gaagatgttt ttagtgttgg ctatgaaact tccgaaaaga ttcatttaat gctcaagtac
     8821 cttttagaaa ataaaaatac cagtacttgg ttagggtctt ctcaatattt aatcaactgg
     8881 tttagtgatg atttaacaaa tgatagtcgg ttggatatcg tatcaccaat ctttgatgac
     8941 ggacttgaag aagatgatga tgacgatacg cctcctgtta taacattagc aactgaagac
     9001 aataaaagaa ttggtaaatc attcatcaag ggacaaaaat tatttgctaa tgatgccact
     9061 tactacgttg ctattttgaa taaaaccagc aatgggcgga ttgctttaaa atattttcgt
     9121 cagcttcaag cgtcccaatt actcaccaat cttaacaagt ggcaggaaac atacagttgg
     9181 gagtcgcgat ctaagtttgg aaaaagtcgc ttaagaaccc ctacttttca tgacatcctt
     9241 aatgtgtcct acggggttga tagggatcgc ttccttgaat tagataatga taacttcaaa
     9301 agtgatcaaa ttcaaaagtt agtggcaagt ttgattgatg gtaaaccgat gccacagtcc
     9361 attgtcaaaa agttaggtaa caatgttaaa gaacgacatc gttaccgtaa gcactggtat
     9421 caagttgagc aggtctgctt agcaatttta cacaaacaaa atggggagga attttcaccg
     9481 atgctagatc ataccaatca aaatcgttcc tatctttttg gacgattatt agcaattttt
     9541 gaattaatcg agaccttgcg ttatggcttg gatggaaaca ataacgaccg tattaccaat
     9601 gctgaacgtt attggacagc ctatactgga caaccaacaa aattgatgat gttattggaa
     9661 aataaaatta agccttacga agaaccattg aaattaaatc gtcgtggcag ttggatgaaa
     9721 ttagaaaaag aaaaagaaga gattttagaa ctgttaaatc ctctgttaga aacagaaaca
     9781 atggaaaaac ccttagatta ccgctttatt tttgggtatt atgctgagaa aaactattac
     9841 tatacaaaac aaaacactga agtaacagaa agtgaggagt aaaaagatgt tggaacacaa
     9901 aattgatttt atggtaactc ttgaagtgaa agaagcaaat gcaaatggtg atcccttaaa
     9961 tggaaacatg cctcgtacag atgccaaagg atatggtgtg atgagtgatg tctccatcaa
    10021 acgtaagatt cgtaatcgtt tgcaagatat ggggaagtct atttttgtgc aagctaatga
    10081 acgtattgaa gatgactttc gttcactaga aagacgcttt tcacaacatt ttacagctaa
    10141 gacacctgac aaagaaattg aagaaaaagc aaatgcatta tggtttgatg ttcgtgcttt
    10201 tggacaagtt tttacttatc tgaaaaaatc aattggggtg cgtggaccag tttccatcag
    10261 tatggctaag tccttggagc caattgtcat ttccagcctt caaattacgc gtagtaccaa
    10321 tggtatggaa gctaagaata atagtggccg ctcttctgat acgatgggga caaaacattt
    10381 tgtagattat ggtgtgtatg tacttaaagg ttctatcaat gcttattttg ctgaaaagac
    10441 tggtttttct caggaagatg ctgaggctat taaagaagtt ttggttagct tgtttgaaaa
    10501 tgatgcgtcg tctgcacgtc cggaaggctc tatgcgagtt tgtgaagtct tttggtttac
    10561 gcattcaagc aaactgggga atgtttcaag tgcgcgtgtc tttgacttgt tagagtatca
    10621 tcaatcaata gaagaaaaaa gcacttatga cgcttatcag attcatctaa atcaagaaaa
    10681 attggctaaa tatgaagcga aagggttaac ggttgaaatc ctagaaggac tctagtatgg
    10741 tctatgccga agatgattat ttaatgctat caggtattca gcatttccaa ttttgtaaac
    10801 gtcaatgggc gttgatccat attgagcaac aatggcttga taatgaagcg acagcgcatg
    10861 gacaggtttt acatactaaa gcagataacc cttacattaa agaaaaacga aaagagcttt
    10921 tggtctcacg tgctatgccc atttcttctg cagaacttgg actttcagga attatggatg
    10981 ttgtggaatt ttataaagat gatcaaggtg tgtctttgag gggaaaacgt gggaaatggt
    11041 taccaaaagt tgtggaatac aagcgcggaa aacctaaaaa agataccaga gatattgtcc
    11101 agttggtggc tcagaccatg tgtttagaag aaacgctaga ctgcgacatt aacgaaggtt
    11161 gtctttatta ccatagtgtc aatcaaagag tgattgttcc tatgacatca gctttgcgtc
    11221 aagaagtgaa ggaattagcc gcagagatgc aagaggttta tcagagtcaa atgctaccta
    11281 aagcagctta ttttaaaaac tgtcagcttt gttctttagt cgatatttgt aagcccaggt
    11341 tgagtaaaaa aacaaggagt gtgtcgcgtt acatcaatga ggctatgacc agtgaggaga
    11401 tggacctatg aaaaagttgc taaatacctt gtatttgacg caagaagatt tttatgtcac
    11461 taaagagggc gataacattg ttatcaagca agaaggtaag gttctcaaac ggtttccgtt
    11521 tcggattatt gacggtattg tctgtttttc ttatttgggt gtgtcgcctg ctttggtgaa
    11581 gttatgtacg gagaatcaga ttaatttatc gtttcataca ccacaagggc gtttttgtgg
    11641 tcgctatatt ggttcaacca atgggaatgt gttgttgcgt agagaacatt atcgtttatc
    11701 tgatcgtgag gaatctttgg aatacgcaaa gcggtttatt ttggctaaaa tttccaactc
    11761 aaggaaatac ttgctacgct ttaaacgaga tcatcgtcaa cagattgata ccaagctttt
    11821 tgaggctgtt aatgacgaat tgatatgggc tttagagatg gttcaggcag cagataataa
    11881 agactcttta agagggattg aaggccaagc tgctaatcag tattttcgca tatttaatga
    11941 cctggtgttg acggacaaaa aaacgtttta cttccaaggt cggagtaaac gaccaccctt
    12001 agattgtgtt aatgccctct tgtcttttgg ttacagttta ctgacctttg aatgtcaatc
    12061 tgccttggaa gctgtcggat tagacagtta cgttggtttc tttcacacgg atcgtcctgg
    12121 gtgtgctagt ttagcgcttg atttagttga agagttccgc tcatatattg tagatcgttt
    12181 tgtcttttca ttaattaata aaggacaact tcagaaaaaa cactttgagg ttaaagaaaa
    12241 tggtagtatt ttaatgacgg aaaatggcag agctattttt attgatttgt ggcagaagcg
    12301 taagcatact gaggtagaac atccttttac aaaagagaaa gtaaaactta tgttattacc
    12361 ctatgtacaa gcgcagcttt tagctaaggc catacgagga gatttagaaa gctatccacc
    12421 ttttatggtt taggagatgt tatatgatgg ttttagtcac ttatgatgta aatacggaaa
    12481 cacctgctgg tagaaaaaga ttgcgtcatg ttgccaaact ctgtgtggac tatgggcaac
    12541 gtgttcaaaa ttctgttttt gaatgttctg tgacacccgc agaatttgtg gatataaagc
    12601 accgcttaac acaaatcatt gatgagaaaa ctgatagtat tcgcttttat ttattgggga
    12661 aaaattggca gaggcgtgtg gaaacacttg gtcgctcaga cagctatgac ccagataaag
    12721 gtgtcttatt attgtaaaaa tctcttgtgc gaagctagct ttcacagaaa caccttgctt
    12781 gctcgcgcaa aaataactta aaaaagaagc gaaatggaga taaaaaggct taaaacacct
    12841 ctatgtgtgc catccgtttc tctataaact gtgtcatttg gcgcagtctc acccttcatg
    12901 ggtgagtgga ttgaaataaa taattacatg actagccaat acacccacat agtctcaccc
    12961 ttcatgggtg agtggattga aagatgaacc cgacctctct tttaatgagg tcgggttttt
    13021 acatgccttt agtcaaccat ttatctgata gcgtatcaaa ttcaagtttt tgcatgttct
    13081 agtctatggt ttgcgccact agctttcggt aaactaaggg taacttaaga gaggagttat
    13141 ttatgacact atcacttgta ttaatggtat cggtttttgg ggcaggcttg ttatcctttt
    13201 tctctccgtg tatctttccg gtccttcctg tttatttagg cattttactg gatgcagatg
    13261 attcaaagac gattactata tttggtaaaa aactctattg gtatggcatt gtcaaaacct
    13321 tagcctttat ttttggtcta tctactattt ttgtgatttt aggttacggg gctggttttt
    13381 taggaaatat cctttatgcg gtatggtttc ggtacttact gggagccttg gtcattatct
    13441 tggggattca tcagatgggc ctcattacca ttaagagctt acaatttcaa aaatcactga
    13501 ctttccataa taacaagaat cgcaacggtt tgttcaatgc atttatcctt ggtttaacct
    13561 ttagctttgg ttggacgcct tgtgtgggac ctgttttgag ttctgttcta gctttggtgg
    13621 cttcaggggg aaatggtgcc tggcaaggtg gcgttttgat gattatttat actcttggat
    13681 tgggcattcc tttcctactc atctcttttg cgtcaggcat tgttttgaaa cagtttaaca
    13741 agctcaaacc ccacatattt ttactgaaaa aagtaggagg cgttctgatt atagtcatgg
    13801 gaatcttgct tatgacagga accttaaata acttagcaca actttttgga taaaggagaa
    13861 acacaatgaa aaaaggacta ttagtaacaa ctggtttggc ttgtctcggg ctactaactg
    13921 cttgctcaac ccaagacaat atggttaaaa aggaaataac tcaggacaag atgagcatgg
    13981 cagttaaaaa gaaagataag atgtcaacat caaaggacaa gtccatgatg gcagataaat
    14041 catctgataa gaaaatgacc aatgatggtc ctatggcgcc tgatttcgaa ctcaaaggca
    14101 tcgatggtaa aacctatcgc ttatcagaat tcaaaggtaa aaaagtttat ttgaaatttt
    14161 gggcctcttg gtgttcaatc tgtttatcaa ccttggcaga taccgaagac ctggctaaaa
    14221 tgtcagataa agactatgtg gtcttaaccg tcgtctcgcc aggtcatcaa ggagaaaaat
    14281 cagaagcaga ctttacaaaa tggttccaag gaacagacta taaggactta ccagtcttgt
    14341 tagatccgga tggcaagcta ttggaagctt acggcgtcag atcttaccca acagaagttt
    14401 tcattggaag tgatggtgtt cttgctaaaa aacacattgg ttatgccaaa aaatcagaca
    14461 tcaaaaagac ccttaaaggt atacattagg aaggaccagc tgataggcat tagaaaaaag
    14521 gagttgagtt tatggaacac aaatggaaat tatggctatt tatcgttgga gtggtcttgt
    14581 taataggcgg agcttctgtt ctcattcaga aaggtcaagc caaggctttt agtcaaggca
    14641 agcctcaact accagctaaa aaagtcaaat ccactaaagt agatcccaat gcccctgttg
    14701 atcaaaaagt gatttacctg gctggaggct gtttctgggg cgttgaagaa tatttttcac
    14761 aagtggatgg cgttctagat gcggtgtcag gctatgccaa tggtagaggc gataccacca
    14821 actaccagtt aattcatcag actggtcatg cagaaaccgt tgaagtgacc tatgatgcga
    14881 accgtattag cttgaaagaa ttattactgc attttttccg aattattgac ccaaccagtc
    14941 ttaataagca aggaaatgat cgcggtagtc aataccgaac tgggatttac tatactgaca
    15001 aagcagattt agctattatt gatgaggtct tcaaagaaaa agccaaggat tacaagaaaa
    15061 aaatagtggt tgaaaaagca ccattgaaac actttattaa ggcagaagac taccaccaag
    15121 attacctcaa gaaaaatcca aatggctact gccatattga catcaaccaa gcgacttatc
    15181 ctgtgattga tgaaagcaag tacccaaaac caagtgccac tgagatcaaa gcaaaactat
    15241 ctgcagacga ataccgagtg acgcaaaaaa acgaaactga aaaagccttt tctaatcgct
    15301 actgggattc ttttgatgca ggaatttacg tagacgttgt gacaggagaa ccgcttttct
    15361 cttcaaaaga caaatttgaa tcaggttgtg gttggccaag ttttagccgc cccatcagcc
    15421 cagacgttgt ccgctacaag gaagataaga gtttcaatat gacgagaaca gaagtgcgca
    15481 gccgttcagg gaattctcat ctggggcatg tctttactga cggacctaaa gaccaaggcg
    15541 gccttcgtta ctgcatcaat agcttgtcta ttacctttat tccaaaagct gatatggaag
    15601 ctaagggcta tggttattta ttatcatctg tagaataggg ttaggcctaa aaatctgata
    15661 taatagaaga aagactagta tgagagggcc atgcaaggcc ctttataaag ataaggagac
    15721 accgtgtgta ctcattgtta attgtagaag acgaatacct tgtgcgccag ggtattcgtt
    15781 ctttggttga ttttagccag ttcaagattg atagggtcaa cgaagcagaa aatggccagt
    15841 tggcttggga cttgtttcag aaagagcctt atgatattgt tttgacggat atcaatatgc
    15901 ccaaattaaa tgggattcaa ctagcagaac tcattaaaca ggaatccccc caaactcatc
    15961 tggtattttt gacgggctac gatgatttta actatgcctt atctgctttg aagttagggg
    16021 cagatgatta cttgctcaaa cccttttcca aggccgatgt ggaagatatg ttaggaaagc
    16081 tccagcaaaa attagacctt tccaagaaaa cagaaaccat ccaggaattg gttgagcagc
    16141 ctcaaaaaga agtatcagca atagcaatgg ctattcatga gcgattggca gattctgatt
    16201 tgaccctaaa aagcttggct cagcagcttg gttttagccc taattacctt agcgtcttga
    16261 ttaaaaaaga tttagggatg ccctttcaag attatctggt acaagaacgg ttgaaaaaag
    16321 ccaagcttct cttgttaacc agtaatctta aaatctatga aattgcagag caggtcggtt
    16381 ttgaggacat gaattatttt tctcagcgct ttaagcaact ggtaggcgtt accccaagtc
    16441 agtataaaaa aggaggccag gcatgaagcg ttatccccta ctggtccagt tgatttcgta
    16501 tgtttttgtg atcgtaattg ccctcattac cacgcttggt ttgctttatt accagacgag
    16561 ttctcgtaat atcaggcaac taattgaacg tgatacccgg cagagcattc ggcaaagttc
    16621 ccaatttatt gacgcttaca tcaagcctct taaagaaaca acttcggtgc tggcgaaaaa
    16681 tacggaaatt caagcctttg ctagtcaaat tcatcaagaa aatgacaaac aggttcttca
    16741 gctcatgaag atggttcttg cgaccaattc cgatttacaa gcagctgttc tggtgactaa
    16801 ggatggtcga acggtgtcta ccaattctca gttgaccatg aaaacctcta gtgacatgat
    16861 ggcagaaccc tggtataaag cagccataga ccgtcaggcc atgccagtct tgaccccagc
    16921 tcggcaatta tccctttctt ctaaaaaaga atgggtagtt tctgtgaccc aagaggtagt
    16981 agatagggct gggcataatt taggtgtgct aagacttgat attgcatacc cgaccattaa
    17041 agcgtcttta gatcagcttc agctaggccg ccaaggcttt gcctttattg tgaatgataa
    17101 gcatgaattt gtttaccatc ccaaaaagag tgtttacagt tcttctaagg agatggctgc
    17161 gatgaagcct tatttagcga ttcagaatgg ttacactaag gacaagacat cttttgttta
    17221 ccaaaaactc attcctaaca gtcaatggac tttagtggga gtggcgtcac tggatcagtt
    17281 gcaccgggtg cagcgccaaa ttttttggtc ctttcttgga acagggcttc tagcctgtct
    17341 gatttgtggc tttgcaactg tcttagcctt acgcagatgg attcgtccca ttcaacaatt
    17401 gcagcaagtt attcttgcta ttcaaaaagg agatcgtcag ttacgtgccc aagaaacggg
    17461 ttctccagaa ttgacaaacc ttgcccaaca gtttaatgcg ctcttagatc aaattgatag
    17521 cttgatggtt gccgttgcgg ataaggaaaa ggcgattggg cagtataggt tacaagcctt
    17581 ggctagtcag attaacccgc attttctcta taacaccttg gacactatta tttggatggc
    17641 agaatttaat gacagcaagc gcgtggtaga agtgaccaag tctctagcta agtattttcg
    17701 tttggccctt aatcagggga acgaatacat tcgtttggca gatgaactgg atcacgttag
    17761 ccaatacctc tttattcaaa aacagcgcta tggagacaag ctaagttatg aagtgcaagg
    17821 cttagatgtc tacgcagact ttgttattcc taagcttatc ttacagccct tagtagaaaa
    17881 tgctatctac catggcatca aagaagtcga tcgcaaaggc atgatcaagg ttacggtatc
    17941 tgatacagct cagcatctgg tgttgactgt ttgggataat ggtaaaggca ttgaagactc
    18001 ttcactgacc aatagtcaga gcttgttggc taggggaggt gtgggcctta aaaatgttga
    18061 ccagcggtta aaacttcact atggtgaagg ctaccacatg accattcata gccagtcaga
    18121 ccagttcact gaaatacaat taacccttcc taaaatgcat gaattaatgg cagacgacac
    18181 acaggaaaat gagtaaggtt acaggaagtg catgtcagcc atttccctaa gttagggtat
    18241 tggagataag aaccttagaa aggatatcat gagtttagca tctgactatt ttagccgaca
    18301 gacccccata gttgaaaagc taatggctta cggttttgaa aaacgaaata atggttactt
    18361 ctataacgaa cggtttatgg aagaagaatt tgaggcgcag cttaggattg atgaggctgg
    18421 caatatctgg gatcgggtta ttgattgtga cttggaggag gattatttgc ctttacaaca
    18481 agcagcctgg caaggaacct atactgggca ggttagagca gcctatttgg aactcttgga
    18541 gcgcttatca gtagcctgtt ttgaagtcac tccttttcag tcaatgcaag ctaatagact
    18601 tgctaagcat atcaccaaag aatggtctga cccaatggac tatccttttg aaaagcaccc
    18661 agaccttgca acctatcggg tagagggcaa atggtatgct atgatttttt ctctcttggc
    18721 tgataaatta gaccagatac cagagagatt agtaggacag acatgtgaag tgatgactgt
    18781 caaagttaat cccaaggctt tccctcagtt actgcagcaa gaaggcattt acccagcgta
    18841 tcacatgtca aagaaaaact ggattagtat tattttggat gataaggtaa cagatgataa
    18901 gctttggacg ttagtaactc aaagtcgtca actagtcaac ccaaacggtc tttctaatcc
    18961 taatggtcct gattattggg ttattccagc taatttgaaa tactatgata ttgatgcaga
    19021 atttgcagcc aatgatgtga ttttatggac ccaaaaagct ggtattgccg tgggagacta
    19081 tgtgctgatt tatataacag ccccagtcaa atccattcgc tatgtctgcc aagttctaga
    19141 aaccaatata cctaacgaag gctatcgtaa aaatcctaac attgacaaac tcatgcgtct
    19201 ccgaaaacgc caacagtaca aggatggtct cttgtctttt gaccttatga aaaaacatgg
    19261 cgtagctgca gtgagagggc caagacgatt aagccctcaa ttaattgcat ttttaaagga
    19321 aaaagagtat tttaaagaga acaactaaag gagaataaga gatggttgaa tttgtagcag
    19381 atcaaagttt ttgggaactt tttccagatg ccaaactagg tgtcgtctta ttaaaggatg
    19441 ttcagaatca agcagagtca ccagaagatg tcaaacagct cttagcagac agccatgagt
    19501 tagcaaaagc ttatttaaca gcagacaatt ttagtgataa tcaggtggtt caagtctacc
    19561 gcaaggccta tcaacacttt aaaaccaaaa aaggagctcg ctctagcatt gaagcccttc
    19621 tcaagcgggt gtcaaatggt caaagcattc cgtcgatcaa tccgctagtg gatatttata
    19681 atgcagctag tctacgtttt ggtttacctg ttggagccga agatagtgac agttttatcg
    19741 gagatttgcg tctgacgata acagacggtg gtgacgactt ttacttgatt ggggatgctg
    19801 acaataaccc aaccttgcca aatgagctgt gttataaaga tgatattgga gcggtttgtc
    19861 gttgccttaa ctggcgagat ggcgaacgca ccatggtgac agaacacacc aaaaatgcat
    19921 ttttgattat cgaagctctt gatcaagaag gtcaaaatag attgcaagaa gctcttgact
    19981 tcattgaagg cagcgcaaaa atgtaccttc atgcaatcac ctctgtgcat gtgttggata
    20041 aagacaatcc acacgtgcca ctttttacgg aaaactagat tagtaggatg aactgtgtaa
    20101 aatagaaggt taacaaaaaa gcgagtaaac gaatgtcctg tcatttaggt ttttgtttac
    20161 tcgctttttg atgagatttt actgctgtcc tagcaaaagg tgaggatgag gtcgtatatc
    20221 acatagaggt tttctgttaa gccttatttg ttactaaaga agaatggtgg tgtgtaatga
    20281 ctaagggtct cgtaacaagc cttggcttgc tcattgtctt cacagacaat caagcaagta
    20341 tcatcaccac agatcgttgc tacaatttct ggaatttgca tggcatctaa aatggagcca
    20401 aaggattggg ctagtccagg taacgttttt aaaataattt gatgttgaac tatctttaac
    20461 atgaccaaag cgtcttccat gtaaaaacga agacgatgtt cccagcgggt ttgtgaaatg
    20521 gctaacgctt catagtgtgt gtcgtttccg gaagttactt tgaccaggtt caattccttc
    20581 atgtcacgtg atagggtagc ctgagtgatc gtgatgccat ttttttgcaa ccgttcttgt
    20641 aattcttgct gtgtatgtat agttgtctca gagatgaggg aacgtatgag ttggtgacgc
    20701 gtctctttct tattcattgt tataatcttc ctttaacgta attgataaca atccgagtat
    20761 agcaaaaaga ggagaaggtg taaagttttt gatgaaccgc ttacaaaatg gcttttttat
    20821 ggtaaaataa agatgatgtt tagaaaaagg agatttgtgt gattcgaaga gaagattatc
    20881 aataccttag aaaactaaac gattttcgct acttttctat tgaacaattt gacaaaattg
    20941 tagggcagat ggaattccga aaagctaaaa aggaccatat cctttttttt gagggagata
    21001 aaagagataa gctttttcta gtgacctctg gttatttcaa ggtggagcag tccgatcaat
    21061 caggaacctt catgtatacg gatttcatca gacacggtac catttttcct tacggtggcc
    21121 tctttacaga tgattattac catttttcag tggtagccat gacagacgtg acttattttt
    21181 actttccagt cgatttattt gaagattatt cgcttgaaaa tcgtctacaa atgaaacact
    21241 tatatagtaa gatgtctaaa ctgctcgagt tacatgagtt gagggttcga aatttgatta
    21301 catcaagtgc tagttctcgt gtgattcagt ctctggcgat tttgctggtt gaaatgggta
    21361 aagacagtga taccttacct tttcaattaa caacaacaga tattgctcaa ataagtggga
    21421 cgacaagaga aactgttagc catgtgctta gggatttgaa gaagcaagaa ttgattacca
    21481 taaaaggtaa gtatttgact tatctagata aagactattt tttacaatac acaaagtgaa
    21541 ttaaaggt
//
LOCUS       DATXIG010000023        21193 bp    DNA     linear   BCT 28-DEC-2023
DEFINITION  TPA_asm: Streptococcus pyogenes strain MGAS11113
            SAMN08555799-rid19682583.denovo.023, whole genome shotgun sequence.
ACCESSION   DATXIG010000023 DATXIG010000000
VERSION     DATXIG010000023.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN08555799
            Sequence Read Archive: SRR6740495
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pyogenes
  ORGANISM  Streptococcus pyogenes
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 21193)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 21193)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-OCT-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-OCT-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001918058.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001918058.2
            Genome Coverage       :: 368x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/12/2023 11:23:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 1,806
            CDSs (total)                      :: 1,769
            Genes (coding)                    :: 1,722
            CDSs (with protein)               :: 1,722
            Genes (RNA)                       :: 37
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1, 1 (5S, 23S)
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 47
            CDSs (without protein)            :: 47
            Pseudo Genes (ambiguous residues) :: 0 of 47
            Pseudo Genes (frameshifted)       :: 30 of 47
            Pseudo Genes (incomplete)         :: 20 of 47
            Pseudo Genes (internal stop)      :: 9 of 47
            Pseudo Genes (multiple problems)  :: 11 of 47
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..21193
                     /organism="Streptococcus pyogenes"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN08555799-rid19682583.denovo.023"
                     /strain="MGAS11113"
                     /serotype="Emm28"
                     /isolation_source="normally sterile site"
                     /host="Homo sapiens"
                     /db_xref="taxon:1314"
                     /country="Finland"
                     /collection_date="2002"
                     /collected_by="National Public Health Laboratory"
     gene            complement(525..911)
                     /gene="rplQ"
                     /locus_tag="VC122_000941"
     CDS             complement(525..911)
                     /gene="rplQ"
                     /locus_tag="VC122_000941"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002887523.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L17"
                     /protein_id="HEP5640876.1"
                     /translation="MAYRKLGRTSSQRKAMLRDLTTDLLINESIVTTEARAKEIRKTV
                     EKMITLGKRGDLHARRQAAAYVRNEIASENYDEATDKYTSTTALQKLFSEIAPRYAER
                     NGGYTRILKTEPRRGDAAPMAIIELV"
     gene            complement(926..1864)
                     /locus_tag="VC122_000942"
     CDS             complement(926..1864)
                     /locus_tag="VC122_000942"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000568977.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit alpha"
                     /protein_id="HEP5640877.1"
                     /translation="MIEFEKPIITKIDENKDYGRFVIEPLERGYGTTLGNSLRRVLLS
                     SLPGAAVTSIKIDGVLHEFDTIPGVREDVMQIILNVKGLAVKSYVEDEKIIELEVEGP
                     AEVTAGDILTDSDIELVNPDHYLFTIAEGHSLRATMTVAKKRGYVPAEGNKKDDAPVG
                     TLAVDSIYTPVKKVNYQVEPARVGSNDGFDKLTIEIMTNGTIIPEDALGLSARVLIEH
                     LNLFTDLTEVAKATEVMKETEKVNDEKVLDRTIEELDLSVRSYNCLKRAGINTVFDLT
                     EKSEPEMMKVRNLGRKSLEEVKVKLADLGLGLKNDK"
     gene            complement(1910..2293)
                     /gene="rpsK"
                     /locus_tag="VC122_000943"
     CDS             complement(1910..2293)
                     /gene="rpsK"
                     /locus_tag="VC122_000943"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003130556.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S11"
                     /protein_id="HEP5640878.1"
                     /translation="MAKPTRKRRVKKNIESGVAHIHATFNNTIVMITDVHGNALAWSS
                     AGALGFKGSRKSTPFAAQMAAEAAAKSAQEHGLKTVEVTVKGPGSGRESAIRALAAAG
                     LEVTAIRDVTPVPHNGARPPKRRRV"
     gene            complement(2311..2676)
                     /gene="rpsM"
                     /locus_tag="VC122_000944"
     CDS             complement(2311..2676)
                     /gene="rpsM"
                     /locus_tag="VC122_000944"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008809894.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S13"
                     /protein_id="HEP5640879.1"
                     /translation="MARIAGVDIPNDKRVVISLTYVYGIGLATSKKILAAAGISEDIR
                     VKDLTSDQEDAIRREVDAIKVEGDLRREVNMNIKRLMEIGSYRGIRHRRGLPVRGQNT
                     KNNARTRKGKAVAIAGKKK"
     gene            complement(2694..2810)
                     /gene="rpmJ"
                     /locus_tag="VC122_000945"
     CDS             complement(2694..2810)
                     /gene="rpmJ"
                     /locus_tag="VC122_000945"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013773089.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L36"
                     /protein_id="HEP5640880.1"
                     /translation="MKVRPSVKPICEYCKVIRRNGRVMVICPTNPKHKQRQG"
     gene            complement(2836..3054)
                     /gene="infA"
                     /locus_tag="VC122_000946"
     CDS             complement(2836..3054)
                     /gene="infA"
                     /locus_tag="VC122_000946"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002262318.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="translation initiation factor IF-1"
                     /protein_id="HEP5640881.1"
                     /translation="MAKEDVIEIEGKVVETMPNAMFTVELENGHQILATVSGKIRKNY
                     IRILVGDRVTVEMSPYDLTRGRITYRFK"
     gene            complement(3172..3810)
                     /locus_tag="VC122_000947"
     CDS             complement(3172..3810)
                     /locus_tag="VC122_000947"
                     /EC_number="2.7.4.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001050422.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenylate kinase"
                     /protein_id="HEP5640882.1"
                     /translation="MNLLIIGLPGAGKGTQAAKIVEEFGVAHISTGDMFRAAMANQTE
                     MGRLAKSYIDKGELVPDEVTNGIVKERLAEDDIAEKGFLLDGYPRTIEQAHALDATLE
                     ELGLRLDGVINIKVDPSCLVERLSGRIINRKTGETFHKVFNPPVDYKEEDYYQREDDK
                     PETVKRRLDVNMAQGEPILEHYRKLGLVTDIEGNQEITDVFADVEKALLELK"
     gene            complement(3960..5264)
                     /gene="secY"
                     /locus_tag="VC122_000948"
     CDS             complement(3960..5264)
                     /gene="secY"
                     /locus_tag="VC122_000948"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015631723.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecY"
                     /protein_id="HEP5640883.1"
                     /translation="MFLKILKDALKIKTVRNKIFFTIFIILVFRIGTHITVPGVNAKS
                     LEQLSELPFLNMLNLVSGNAMRNFSVFSMGVSPYITASIVVQLLQMDILPKFVEWGKQ
                     GEVGRRKLNQATRYISLVLAFAQSIGITAGFNTLSNVALVKTPDIKTYLLIGALLTTG
                     SVIVTWLGEQITDKGFGNGVSMIIFAGIISSIPSAIATIREDYFVNVKASDLHSSYLI
                     VGILIIAVLAIVFFTTYVQQAEYKIPIQYTKLMQGAPTSSYLPLKVNPAGVIPVIFAS
                     SITTIPSTIIPFVQNGRDLPWLNRLQEIFNYQTPVGMIVYALLIILFSFFYTFVQVNP
                     EKTAENLQKNSSYIPSVRPGRETEQFMSALLKKLATVGAIFLAFISLAPIAAQQALNL
                     SSSIALGGTSLLILISTGIEGMKQLEGYLLKRKYVGFMNTAE"
     gene            complement(5281..5721)
                     /gene="rplO"
                     /locus_tag="VC122_000949"
     CDS             complement(5281..5721)
                     /gene="rplO"
                     /locus_tag="VC122_000949"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004225266.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L15"
                     /protein_id="HEP5640884.1"
                     /translation="MKLHELKAAEGSRKVRNRVGRGTSSGNGKTSGRGQKGQKARSGG
                     GVRLGFEGGQTPLFRRIPKRGFTNINTKEYALVNLDQLNVFDDGTEVTPAILKDAGIV
                     RAEKSGVKVLGNGELTKKLTVKAAKFSKSAEAAIIAKGGSIEVI"
     gene            complement(5936..6118)
                     /gene="rpmD"
                     /locus_tag="VC122_000950"
     CDS             complement(5936..6118)
                     /gene="rpmD"
                     /locus_tag="VC122_000950"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002959367.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L30"
                     /protein_id="HEP5640885.1"
                     /translation="MAQIKITLTKSPIGRKPEQRKTVVALGLGKLNSSVVKEDNAAIR
                     GMVTAISHLVTVEDVK"
     gene            complement(6133..6627)
                     /gene="rpsE"
                     /locus_tag="VC122_000951"
     CDS             complement(6133..6627)
                     /gene="rpsE"
                     /locus_tag="VC122_000951"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018376782.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S5"
                     /protein_id="HEP5640886.1"
                     /translation="MAFKDNAVELEERVVAINRVTKVVKGGRRLRFAALVVVGDGNGR
                     VGFGTGKAQEVPEAIRKAVEAAKKNMIEVPMVGTTIPHEVYTNFGGAKVLLKPAVEGS
                     GVAAGGAVRAVIELAGVADITSKSLGSNTPINIVRATVEGLKQLKRAEEVAALRGISV
                     SDLA"
     gene            complement(6646..7002)
                     /gene="rplR"
                     /locus_tag="VC122_000952"
     CDS             complement(6646..7002)
                     /gene="rplR"
                     /locus_tag="VC122_000952"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018374469.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L18"
                     /protein_id="HEP5640887.1"
                     /translation="MISKPDKNKIRQKRHRRVRGKLSGTADRPRLNVFRSNTGIYAQV
                     IDDVAGVTLASASTLDKDVSKGTKTEQAVVVGKLVAERAVAKGISEVVFDRGGYLYHG
                     RVKALADAARENGLKF"
     gene            complement(7107..7643)
                     /gene="rplF"
                     /locus_tag="VC122_000953"
     CDS             complement(7107..7643)
                     /gene="rplF"
                     /locus_tag="VC122_000953"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016399261.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L6"
                     /protein_id="HEP5640888.1"
                     /translation="MSRIGNKVITMPAGVELTNNNNVITVKGPKGELTREFNKNIEIK
                     VEGTEITVVRPNDSKEMKTIHGTTRANLNNMVVGVSEGFKKDLEMKGVGYRAQLQGTK
                     LVLSVGKSHQDEVEAPEGITFTVANPTSISVEGINKEVVGQTAAYIRSLRSPEPYKGK
                     GIRYVGEYVRLKEGKTGK"
     gene            complement(7846..8244)
                     /gene="rpsH"
                     /locus_tag="VC122_000954"
     CDS             complement(7846..8244)
                     /gene="rpsH"
                     /locus_tag="VC122_000954"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009754342.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S8"
                     /protein_id="HEP5640889.1"
                     /translation="MVMTDPIADFLTRIRNANQVKHEVLEVPASNIKKGIAEILKREG
                     FVKNVEVIEDDKQGIIRVFLKYGKNGERVITNLKRISKPGLRVYAKRDDMPKVLNGLG
                     IAIISTSEGLLTDKEARQKNVGGEVIAYVW"
     gene            complement(8395..8580)
                     /locus_tag="VC122_000955"
     CDS             complement(8395..8580)
                     /locus_tag="VC122_000955"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011245027.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type Z 30S ribosomal protein S14"
                     /protein_id="HEP5640890.1"
                     /translation="MAKKSMIAKNKRPAKHSTQAYTRCEKCGRPHSVYRKFKLCRVCF
                     RELAYKGQIPGVVKASW"
     gene            complement(8596..9138)
                     /gene="rplE"
                     /locus_tag="VC122_000956"
     CDS             complement(8596..9138)
                     /gene="rplE"
                     /locus_tag="VC122_000956"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018376779.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L5"
                     /protein_id="HEP5640891.1"
                     /translation="MANRLKEKYTNEVIPALTEKFNYTSVMAVPKVEKIVLNMGVGDA
                     VSNAKNLEKAAAELALISGQKPLITKAKKSIAGFRLREGVAIGAKVTLRGERMYEFLD
                     KLVSVSLPRVRDFHGVPTKSFDGRGNYTLGVKEQLIFPEISFDDVDKVRGLDIVIVTT
                     ANTDEESRELLKGLGMPFAK"
     gene            complement(9162..9467)
                     /gene="rplX"
                     /locus_tag="VC122_000957"
     CDS             complement(9162..9467)
                     /gene="rplX"
                     /locus_tag="VC122_000957"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011921687.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L24"
                     /protein_id="HEP5640892.1"
                     /translation="MFVKKGDKVRVIAGKDKGTEAVVLKALPKVNKVIVEGVGMIKKH
                     QKPNTENPQGAIVEKEAPIHVSNVQVLDKNGVAGRVGYKVVDGKKVRYSKKSGEVLD"
     gene            complement(9546..9914)
                     /gene="rplN"
                     /locus_tag="VC122_000958"
     CDS             complement(9546..9914)
                     /gene="rplN"
                     /locus_tag="VC122_000958"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012727667.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L14"
                     /protein_id="HEP5640893.1"
                     /translation="MIQQETRLKVADNSGAREILTIKVLGGSGRKFANIGDVIVASVK
                     QATPGGAVKKGDVVKAVIVRTKTGARRPDGSYIKFDDNAAVIIRDDKTPRGTRIFGPV
                     ARELREGGYMKIVSLAPEVL"
     gene            complement(9939..10199)
                     /gene="rpsQ"
                     /locus_tag="VC122_000959"
     CDS             complement(9939..10199)
                     /gene="rpsQ"
                     /locus_tag="VC122_000959"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006738952.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S17"
                     /protein_id="HEP5640894.1"
                     /translation="MERNQRKTLYGRVVSDKMDKTITVVVETKRNHPVYGKRINYSKK
                     YKAHDENNVAKEGDIVRIMETRPLSATKRFRLVEVVEKAVII"
     gene            complement(10225..10431)
                     /locus_tag="VC122_000960"
     CDS             complement(10225..10431)
                     /locus_tag="VC122_000960"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006739716.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L29"
                     /protein_id="HEP5640895.1"
                     /translation="MKLQEIKDFVKELRGLSQEELAKKENELKKELFDLRFQAAAGQL
                     EKTARLDEVKKQIARVKTVQSEMK"
     gene            complement(10441..10854)
                     /gene="rplP"
                     /locus_tag="VC122_000961"
     CDS             complement(10441..10854)
                     /gene="rplP"
                     /locus_tag="VC122_000961"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003029477.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L16"
                     /protein_id="HEP5640896.1"
                     /translation="MLVPKRVKHRREFRGKMRGEAKGGKEVSFGEYGLQATTSHWITN
                     RQIEAARIAMTRYMKRGGKVWIKIFPHKSYTAKAIGVRMGSGKGAPEGWVAPVKRGKV
                     MFEIAGVSEEIAREALRLASHKLPVKCKFVKREAE"
     gene            complement(10858..11511)
                     /gene="rpsC"
                     /locus_tag="VC122_000962"
     CDS             complement(10858..11511)
                     /gene="rpsC"
                     /locus_tag="VC122_000962"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000529931.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S3"
                     /protein_id="HEP5640897.1"
                     /translation="MGQKVHPIGMRVGIIRDWDAKWYAEKEYADYLHEDLAIRKFINK
                     ELADASVSTIEIERAVNKVIVSLHTAKPGMVIGKGGANVDALRGQLNKLTGKQVHINI
                     IEIKQPDLDAHLVGENIARQLEQRVAFRRAQKQAIQRTMRAGAKGIKTQVSGRLNGAD
                     IARAEGYSEGTVPLHTLRADIDYAWEEADTTYGKLGVKVWIYRGEVLPARKNTKGGK"
     gene            complement(11524..11868)
                     /gene="rplV"
                     /locus_tag="VC122_000963"
     CDS             complement(11524..11868)
                     /gene="rplV"
                     /locus_tag="VC122_000963"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018371349.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L22"
                     /protein_id="HEP5640898.1"
                     /translation="MAEITSAKAMARTVRVSPRKTRLVLDLIRGKKVADAIAILKFTP
                     NKAARVIEKTLNSAIANAENNFGLEKANLVVSETFANEGPTMKRFRPRAKGSASPINK
                     RTTHVTVVVSEK"
     gene            complement(11884..12162)
                     /gene="rpsS"
                     /locus_tag="VC122_000964"
     CDS             complement(11884..12162)
                     /gene="rpsS"
                     /locus_tag="VC122_000964"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000533765.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S19"
                     /protein_id="HEP5640899.1"
                     /translation="MGRSLKKGPFVDEHLMKKVEAQANDEKKKVIKTWSRRSTIFPSF
                     IGYTIAVYDGRKHVPVYIQEDMVGHKLGEFAPTRTYKGHAADDKKTRR"
     gene            complement(12302..13135)
                     /gene="rplB"
                     /locus_tag="VC122_000965"
     CDS             complement(12302..13135)
                     /gene="rplB"
                     /locus_tag="VC122_000965"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019790357.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L2"
                     /protein_id="HEP5640900.1"
                     /translation="MGIKVYKPTTNGRRNMTSLDFAEITTSTPEKSLLVSLKSKAGRN
                     NNGRITVRHQGGGHKRHYRLIDFKRNKDGVEAVVKTIEYDPNRTANIALVHYTDGVKA
                     YIIAPKGLEVGQRIVSGPDADIKVGNALPLANIPVGTVVHNIELKPGKGGELVRAAGA
                     SAQVLGQEGKYVLVRLQSGEVRMILGTCRATIGTVGNEQQSLVNIGKAGRSRWKGIRP
                     TVRGSVMNPNDHPHGGGEGKAPVGRKAPSTPWGKPALGLKTRNKKAKSDKLIVRRRNE
                     K"
     gene            complement(13153..13449)
                     /locus_tag="VC122_000966"
     CDS             complement(13153..13449)
                     /locus_tag="VC122_000966"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006739217.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L23"
                     /protein_id="HEP5640901.1"
                     /translation="MNLYDVIKKPVITEKSMIALEAGKYTFEVDTRAHKLLIKQAVEA
                     AFDGVKVASVNTVNVKPKAKRVGRYTGFTSKTKKAIITLTADSKAIELFAAEAE"
     gene            complement(13449..14072)
                     /gene="rplD"
                     /locus_tag="VC122_000967"
     CDS             complement(13449..14072)
                     /gene="rplD"
                     /locus_tag="VC122_000967"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012677212.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L4"
                     /protein_id="HEP5640902.1"
                     /translation="MANVKLFDQTGKEVSSVELNDAIFGIEPNESVVFDVVISQRASL
                     RQGTHAVKNRSAVSGGGRKPWRQKGTGRARQGSIRSPQWRGGGVVFGPTPRSYGYKLP
                     QKVRRLALKSVYSAKVAEDKFVAVEGLSFAAPKTAEFAKVLSALSIDTKVLVLVEEGN
                     EFAALSARNLPNVTVATAATASVLDIVNADKLLVTKEAISTIEEVLA"
     gene            complement(14096..14722)
                     /gene="rplC"
                     /locus_tag="VC122_000968"
     CDS             complement(14096..14722)
                     /gene="rplC"
                     /locus_tag="VC122_000968"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002986659.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L3"
                     /protein_id="HEP5640903.1"
                     /translation="MTKGILGKKVGMTQIFTESGEFIPVTVIEATPNVVLQVKTVETD
                     GYEAVQVGFDDKREVLSNKPAKGHVAKANTAPKRFIREFKNIEGLEVGAELSVEQFEA
                     GDVVDVTGTSKGKGFQGVIKRHGQSRGPMAHGSRYHRRPGSMGPVAPNRVFKNKRLAG
                     RMGGNRVTVQNLEIVQVIPEKNVILIKGNVPGAKKSLITIKSAVKAAK"
     gene            complement(14970..15278)
                     /gene="rpsJ"
                     /locus_tag="VC122_000969"
     CDS             complement(14970..15278)
                     /gene="rpsJ"
                     /locus_tag="VC122_000969"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003046044.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S10"
                     /protein_id="HEP5640904.1"
                     /translation="MANKKIRIRLKAYEHRTLDTAAEKIVETATRTGATVAGPVPLPT
                     ERSLYTIIRATHKYKDSREQFEMRTHKRLVDIINPTQKTVDALMKLDLPSGVNVEIKL
                     "
     gene            complement(15477..16766)
                     /locus_tag="VC122_000970"
     CDS             complement(15477..16766)
                     /locus_tag="VC122_000970"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014635223.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MATE family efflux transporter"
                     /protein_id="HEP5640905.1"
                     /translation="MIYNRRKIFSLALPSMIENILQMLMGMVDNYLVAQIGLVAVSGV
                     SIANNIISIYQSLFIALGAAVSSLIARSIGENNQNKQLNYMAGVLQVTLLLSVGLGLL
                     SVVGHHQVLEWLGAEASVTLVGGQYLSIVGGMIVSLGLLTSLGAIVRAQGYPKIPMQV
                     SLLINVLNAIFSALSIYVWGFGLLGVAWATVLSRLVGVFLLCQFIPIKQVAKRLMRPL
                     DKIIFDLSLPAAGERLMMRAGDVLIIGIVVRFGTTALAGNAIGETLTQFNYMPGLAMA
                     TATIILVARQLGGGKVTEIRYIIREAFILSTLMMLVMGALTYLLGPSLLPLFTQNTDA
                     QRSAMVVLLFSLLGAPATAGTLVYTAVWQGLGKAKLPFYATTIGMWVIRIGLGYVIGV
                     VWQYGLIGVWMATVLDNTSRWFILSKQFKKYQEITFH"
     gene            complement(17154..18170)
                     /gene="adhP"
                     /locus_tag="VC122_000971"
     CDS             complement(17154..18170)
                     /gene="adhP"
                     /locus_tag="VC122_000971"
                     /EC_number="1.1.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011017243.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alcohol dehydrogenase AdhP"
                     /protein_id="HEP5640906.1"
                     /translation="MKAVVVNQASTGVEVVEHDLPNVGHGEALVKVEYCGVCHTDLHV
                     AHGDFGQVPGRILGHEGIGIVEEIGEGVTSLKVGDRVSIAWFFEGCGHCEYCTTGRET
                     LCRSVKNAGYSVDGGMSEYAVVTADYAVKVPEGLDPAQASSITCAGVTTYKAIKEAGA
                     APGQWIVIFGAGGLGNLAVQYAKKVFNAHVVAVDINNDKLELAKEVGADILVNGKEIE
                     DVPGYIQEKTGGAHGVVVTAVSKVAFNQAIDSVRAGGTVVAVGLPSEYMELSIVKTVL
                     DGIKVVGSLVGTRKDLEEAFAFGAEGLVAPVVEKVPVDTAPEVFDEMERGLIQGRKVL
                     DFIS"
     gene            complement(18422..21064)
                     /gene="adhE"
                     /locus_tag="VC122_000972"
                     /gene_synonym="adhC"
     CDS             complement(18422..21064)
                     /gene="adhE"
                     /locus_tag="VC122_000972"
                     /gene_synonym="adhC"
                     /EC_number="1.1.1.1"
                     /EC_number="1.2.1.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011888528.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional acetaldehyde-CoA/alcohol
                     dehydrogenase"
                     /protein_id="HEP5640907.1"
                     /translation="MTEGHNTVETTSVSVTINALVQKGLAALEEMRKLDQEQVDYIVA
                     KASVAALDAHGELAKHAYKETGRGVFEDKATKNLFACEHVVNNMRHQKTVGIIEEDDV
                     TGLTLIAEPVGVICGITPTTNPTSTAIFKSLISLKTRNPIIFAFHPSAQESSAHAARI
                     VRDAAIAAGAPENCVQWVETPSLEATNALMNHDGIATILATGGNAMVKAAYSCGKPAL
                     GVGAGNVPAYVEKSANIRQAAHDIVMSKSFDNGMVCASEQAVIIDKEIYDDFVAEFKS
                     YHTYFVNKKEKALLEEFCFGAKANSKNCAGAKLNPNIVGKPATWIAEQAGFTVPEGTN
                     ILAAECKEVSENEPLTREKLSPVIAVLKSESREDGVEKARQMVEFNGLGHSAAIHTAD
                     AELAKEFGTRIRAIRVIWNSPSTFGGIGDVYNAFLPSLTLGCGSYGRNAVGDNVSAIN
                     LLNIKKVGRRRNNMQWFKVPSKTYFERDSIQYLQKCRDVERVMIVTDHAMVELGFLDR
                     IIEQLDLRRNKVVYQIFAEVEPDPDITTVMKGTELMRTFKPDTIIALGGGSPMDAAKV
                     MWLFYEQPEVDFHDLVQKFMDIRKRAFKFPELGKKTKFVAIPTTSGTGSEVTPFAVIS
                     DKANNRKYPIADYSLTPTVAIVDPALVLTVPGFIAADTGMDVLTHATEAYVSQMANDF
                     TDGLALQAIKIVFDNLEKSVKIADFEAREKMHNASTMAGMAFANAFLGISHSMAHKIG
                     AQFHTVHGRTNAILLPYVIRYNGTRPAKTATWPKYNYYRADEKYQDIAKLLGLPASTP
                     EEAVESYAKAVYDLGCRVGIQMNFKAQGIDENEWKEHSRELAYLAYEDQCSPANPRLP
                     MVDHMQEIIEDAYYGYAERPGRRK"
BASE COUNT         6105 a         4491 c         3823 g         6774 t
ORIGIN      
        1 aattttgttg gaaacggctt aaagtgacag attgtaaaag taaaaaagat attttcacct
       61 ccaaattaaa agaggatttt aaaatactta ttaataaaat attgaatagg ggataaaact
      121 aacaatatag tttgagcatc tgtttgcgat gcaagttttt ttcggacatt taaacgaggg
      181 gattacaccg tttatttgcc catgagaggt ctttaaacac ctgcataagg ttttcttatt
      241 ctgatgacct tattgtctct gagaaaactt tataaacgca tcagaagagg cttctgtgtt
      301 attgttttac tcagcttaaa agcgggagtg tcacctcata atacaacaaa acgcctgctt
      361 ataaaaagtt tctatagctt agataccttt cataaacaag cgtcacatat taacaatatg
      421 atgagtgttc ccgctaggaa aaatcctagc ggtagaccag agctagacta agaataatct
      481 ctcgattact ccatcatcat aacactcaac aaaattgata aaaattaaac taattcaata
      541 attgccattg gagcagcatc tccacggcgt ggttctgttt taagaatacg agtgtatcca
      601 ccgttacgtt ctgcataacg aggtgcaatt tctgagaaaa gtttttgaag agctgtcgtt
      661 gatgtgtatt tatcggtagc ttcatcataa ttttctgatg cgatttcgtt acgaacgtat
      721 gcagctgctt ggcgacgagc atgaagatca ccacgtttac caagagtaat cattttttca
      781 actgttttac ggatttcttt tgcacgtgct tctgtcgtta caattgattc gttgatcaaa
      841 agatctgtcg tcaaatcacg aagcattgct ttacgttgtg agctagtgcg tcctagttta
      901 cggtaagcca ttttgtcctc ctatattatt tatcgttttt tagtccgaga cctaagtcag
      961 caagtttaac cttaacttct tcaaggctct tacgtccaag attacggact ttcatcattt
     1021 caggctcaga tttttctgtt aagtcgaaga cagtgttaat gcctgcacgt tttaaacagt
     1081 tatatgagcg tacggataaa tcaagctcct cgatggtgcg gtcaagtacc ttttcatcgt
     1141 tcactttttc agtttctttc attacttcgg tagcttttgc aacctctgtt aaatcagtaa
     1201 acaggtttaa gtgttcgatc aaaactcgag cagatagacc taaagcatct tcaggaataa
     1261 ttgttccgtt tgtcatgatt tcaatcgtta atttatcaaa gccatcattg ctacctacac
     1321 gggcaggttc aacttgataa ttaacttttt taactggcgt atagattgaa tctactgcca
     1381 atgtacccac aggtgcatca tcttttttgt ttccttctgc tggcacataa ccacgttttt
     1441 tagcaacagt cattgttgca cgtagagaat gtccttcagc gattgtaaaa agataatgat
     1501 ccgggttaac aagttcgata tcactgtcag taagaatatc accagctgtt acctctgctg
     1561 gtccttctac ttcaagttcg ataatctttt cgtcttcgac gtatgatttc acagcaagtc
     1621 ccttaacatt aaggataatt tgcatgacat cttcacgtac acctgggatt gtatcaaatt
     1681 cgtgtagtac tccatcaatt ttaattgatg ttactgctgc acctggaagt gaagacaaga
     1741 gtacacgacg aagagaatta cctagagttg ttccgtagcc acgttcaagt ggttcgatga
     1801 caaatctacc gtaatcttta ttttcatcaa tttttgttat tattggtttt tcaaactcaa
     1861 tcattttttc acccctcgaa acgaaacttg tgtactattt gttatatgat tatacacgac
     1921 gacgttttgg aggacgagca ccattatgtg gcacaggggt cacatcacga attgcagtta
     1981 cttcaagacc tgcagctgct agagcacgaa tagctgattc acgacctgaa ccaggacctt
     2041 ttacagtcac ttcaacagtt tttagtccgt gttcttgtgc agattttgca gcagcttctg
     2101 cagccatttg agcagcaaat ggagtagatt tacgagaacc tttgaaacca agagcaccag
     2161 ctgatgacca tgcaagagca ttaccatgca catctgtaat cataacaata gtgttattaa
     2221 atgtagcgtg aatgtgtgca acaccagatt cgatgttctt tttcacacga cgtttacgtg
     2281 ttggtttagc caattgtttt acctcctatt ttattttttc ttacctgcaa tcgcaacagc
     2341 tttcccttta cgagtgcgag cattgttttt agtattttgt ccacggacag gaagtccacg
     2401 acgatgacgg attccacggt atgaaccgat ttccatcaaa cgtttaatgt tcatgtttac
     2461 ttcacgtcga aggtcacctt caactttgat tgcatccact tcacgacgga tagcatcttc
     2521 ttgatctgat gttaaatctt taacacggat atcttcagaa atacctgcag ctgctaagat
     2581 ttttttagat gttgcaagac caattccgta aacataagta agtgaaatta ctacgcgttt
     2641 atcatttgga atatcaactc cagcaatacg agccattttt tctcctttct attttatcct
     2701 tgacgttgtt tgtgttttgg atttgtcgga caaattacca taacacgacc attacggcga
     2761 ataactttac agtattcgca aattggttta accgatggtc ttaccttcat gttttaatcc
     2821 ctccaattat ttcgattatt taaagcggta tgtgatgcgt ccacgtgtca agtcataagg
     2881 actcatttct actgtaacac ggtcacctac taaaatacga atgtaatttt tgcggatttt
     2941 tcctgataca gttgctagaa tttgatgtcc attttccaat tcaacagtaa acattgcatt
     3001 tggcatcgtt tctactactt tgccttcaat ttcaatcacg tcttcttttg ccacgcaaaa
     3061 gtacccccta aatttcgatt tgatgtcctc tgagcacaga ggtaacaatt ataagtcaga
     3121 ctaaactagt atatcattac taatctactt atgcaagtag attagtataa tttatttgag
     3181 ttctagcaac gctttttcaa catctgcaaa aacgtctgtt atttcttgat taccttcaat
     3241 atctgtaaca agaccaagct tacgatagtg ttcaagaata ggttctcctt gagccatatt
     3301 gacgtccaaa cgacgtttaa cagtttcagg cttatcatct tcacgttgat agtaatcttc
     3361 ttctttataa tctactggtg ggttgaacac tttatggaac gtttcaccag ttttacgatt
     3421 gataatacga ccactcaaac gctctacaag acatgatgga tccactttaa tattaataac
     3481 accatctaag cggagtccta gttcttcaag tgttgcatct aaggcgtgtg cttgttcaat
     3541 agtacgtgga tacccatcaa gcaaaaaacc tttttctgcg atatcatcct cagctaagcg
     3601 ctcttttaca atcccgtttg tgacttcatc aggaaccaat tcacctttat caatataact
     3661 tttagctaaa cgtcccattt cggtttgatt agccattgcg gcgcggaaca tatcccctgt
     3721 tgagatgtga gcaacaccaa attcttcaac aatcttagct gcttgggtcc ctttaccagc
     3781 tcccggcaaa cctatgatta aaagattcat cttttcctcc tcttttaaaa taaatacata
     3841 gtaggaaatt attttccttg gtttttgttt tcaaatggca acaagaaatc gtttgatgat
     3901 aaccaagtct tattgtcatt taaaaacaaa atagaaggtt gacaatgtca acctgtattc
     3961 tattctgctg tattcataaa tccgacatat tttctcttta gaagataccc ctcaagctgt
     4021 ttcatacctt cgataccagt tgaaatcaaa atgagcaaac tagttccacc taaagcaata
     4081 ctagaagaaa ggttgagagc ttgttgtgct gctattggcg ctaaagagat aaatgctaag
     4141 aagatagctc ctacggttgc tagttttttt agcaatgcgg acataaattg ttctgtctca
     4201 cgtccagggc gaacacttgg tatatatgag gaattcttct gaagattttc tgctgtcttc
     4261 tcaggattaa cttgtacaaa ggtatagaag aatgagaaca atataatcaa caaggcgtaa
     4321 actatcattc cgactggagt ttgataatta aaaatttctt gcaaacggtt taaccacggt
     4381 aaatctctac cattttggac aaaaggaata atcgtacttg ggatagttgt aatcgagcta
     4441 gcaaagataa cgggaataac gccggctgga tttactttta atggaagata tgaacttgta
     4501 ggtgcacctt gcattagctt tgtgtattgg attggaattt tatattccgc ttgttggaca
     4561 tatgttgtaa agaaaacaat agcaagaaca gctatgatta aaatcccaac aatgagataa
     4621 gacgagtgta aatcacttgc cttaacattt acaaaatagt cttcacgaat tgttgcaatt
     4681 gcacttggaa tagacgaaat aatacccgca aagataatca ttgatacgcc attaccaaat
     4741 cctttatcgg tgatttgttc tccaagccag gtaacaataa cgctacctgt tgtcagtaat
     4801 gccccgatca gtaaatatgt tttgatgtct ggtgtcttaa caagtgcaac gttcgacaaa
     4861 gtattaaacc ctgcagtgat accaatggat tgggcaaaag ccaaaacaag tgaaatatag
     4921 cgcgtcgctt gatttaattt acgacgtcca acctcacctt gtttgcccca ctcaacaaac
     4981 ttaggtaaaa tatccatctg caataactga acaacgatag aagcagtaat atatgggcta
     5041 acccccattg aaaatactga gaaatttctc atcgcattac cactaactaa gtttaacata
     5101 tttaagaaag ggagttcact taattgctct aagctcttgg catttacacc aggtacggta
     5161 atatgtgtcc caattcggaa tacaagaatg ataaatatag taaagaaaat tttattccgt
     5221 acagtcttta tctttaacgc gtcttttagt atttttaaga acataatgag tatcccctca
     5281 ttagatgact tcgattgaac cacctttagc aataatagct gcttcagcag attttgagaa
     5341 cttagctgct tttacagtca attttttagt caattcaccg ttaccaagca ctttaacgcc
     5401 tgatttttca gctcggacaa taccagcatc ttttaaaata gccggagtca cttctgtacc
     5461 atcatcaaaa acattcagtt gatcaagatt cacaagtgca tattctttag tattaatatt
     5521 tgtaaagcca cgttttggaa tacgacggaa caatggtgtt tgtccacctt caaaacctaa
     5581 acgtacaccg ccaccgctac gagctttttg acctttttga ccgcgaccag atgttttacc
     5641 attacctgat gatgtcccac gaccaacacg gttacgtact ttacgtgagc cttcagcagc
     5701 ttttaattca tgaagtttca ttttttctcc tttttttgta aaatgctagc gcctctatga
     5761 gggaaaagga ctccctatag aaactcgcct atacattaat gatacttaag ttatccaaaa
     5821 taacttaata cagatacgca ctaaccgact actgtttgtt gccaaaaaag caaactacaa
     5881 gagtcggttt tgtgctcgtt tttaattatg aaatcagaga tttcacagtt ataacttatt
     5941 taacatcttc aacggtaacc aagtgagaaa tagctgtcac cataccacgg atagcagcat
     6001 tatcttcttt gactactgaa gagtttaatt taccaagtcc aagagcaaca acagttttac
     6061 gttgttctgg cttacgtccg ataggagact tagtcaaagt aattttaatt tgagccatga
     6121 tgatcccctt tcttatgcta agtcagaaac tgagatgcca cgaagtgcgg caacttcttc
     6181 tgcacgttta agttgtttca atccttcaac agttgcacga acaatgttga ttggagtgtt
     6241 tgaaccaaga gattttgaag taatatcagc aacacctgct aattcgatga cggcacgaac
     6301 tgcaccacca gcagcaactc cagaaccttc tacagctggt ttcaataata ctttagctcc
     6361 accgaagtta gtgtaaactt cgtgagggat tgttgtacca accattggta cttcgatcat
     6421 gtttttctta gcagcttcaa ctgctttacg gatagcttct ggaacttctt gagctttacc
     6481 agtaccaaat ccaacacgac catttccatc accaacaact acaagagctg caaagcgaag
     6541 acgacgtcca cctttaacaa cttttgtaac gcggttaatc gcaacaacgc gttcttcaag
     6601 ttcaactgca ttatctttaa atgccattat tttgtgtcct ccctattaga atttcaatcc
     6661 gttttcacga gctgcatcag ccaaggcttt aacacgtccg tgatagagat atccaccgcg
     6721 gtcaaacacc acttcagaaa tacctttagc cactgcacgt tcagcaacaa gtttgccgac
     6781 tacaacggct tgttctgttt ttgttccttt agaaacgtct ttatcaagag ttgatgcact
     6841 tgcgagcgtt acacccgcta cgtcatcaat aacttgagcg tagatgcctg tattagaacg
     6901 gaatacgttc aaacgtgggc gatcagcagt tccagagagt ttaccgcgga cgcgacggtg
     6961 gcgtttttgg cggattttgt ttttatctgg tttcgaaatc acaattttca cctcttaatt
     7021 ttaatcaatt tgtctcattg acaagttgtc gattgaactt atcgagcatg ttaaggtgca
     7081 atacttccca tacccgacta agaacattat ttacctgttt taccttcttt aaggcgtacg
     7141 tattcaccaa cgtaacggat ccctttgcct ttgtaaggct ctggtgaacg caagctacgg
     7201 atgtaagcag ctgtttgacc aacaacttct ttgttgattc cttcaactga gattgaagtt
     7261 gggttagcaa cagtgaaagt aattccttct ggagcttcaa cttcgtcttg gtgagactta
     7321 cctactgaaa ggacaagttt agtaccttga agttgagcac ggtaaccgac acccttcatt
     7381 tcaagatctt ttttgaaacc ttcagaaaca cctacaacca tgttattcaa gttagcacgg
     7441 gttgtcccat ggattgtttt catttctttt gagtcgttag gacgtacaac tgtgatttca
     7501 gtcccttcaa ctttgatttc aatatttttg ttgaactcac gagtgagttc gcctttaggg
     7561 cctttaacag taataacatt gttgttattt gttaattcaa cgcctgcagg catagtaatt
     7621 actttattac caatacgtga catgtttatt ttctcctgtt aaattgtcaa gccgcatagc
     7681 gactagtttt cacgggggtt tgatagaatg atatacttca atttatattt caaaattgaa
     7741 cacgagctgt ggtctgtgtg aaaaagataa atcttccttg agtgtaaaca cgctacgtca
     7801 gatttcctat tttcactttg tccacgacgc tcttagtatc ttaaattacc aaacgtaagc
     7861 gatcacttct ccaccaacat tcttttggcg agcttctttg tcagtcaaaa gaccttctga
     7921 tgttgaaatg attgcaattc caagtccgtt aagaacttta ggcatgtcat cacgttttgc
     7981 gtaaacgcga agacctggtt ttgaaatacg ttttaagtta gtgataacac gctcaccatt
     8041 ttttccgtat tttaagaaca cacgaatgat gccttgttta tcatcttcga taacttcaac
     8101 attttttaca aaaccttcac gtttaagaat ctcagcaatc ccttttttaa tgtttgaagc
     8161 aggtacttct aacacttcgt gttttacttg attagcatta cggatacgtg ttaaaaagtc
     8221 tgcaattggg tcagtcataa ccatttttta tattctcctc ttactagcag tttgatgcta
     8281 tcacttacta gttaatgttg tccttaaagg actttggtat cagcttatgc tcacaccgtt
     8341 ttaattaagc acaagactta atctgaagaa caaattaagt tttgtgtcat atttttacca
     8401 agaagctttg acaacacctg gaatttgacc tttgtaagcc aattcacgga agcaaacacg
     8461 acaaagttta aacttgcggt aaactgaatg tggacgtcca catttttcac agcgagtata
     8521 agcttgtgta gagtgttttg caggacgttt gttcttagca atcatagatt ttttagccaa
     8581 tttatttacc ccctattatt ttgcaaaagg cattccaagg cccttaagca attcacgtga
     8641 ttcttcgtca gtatttgcag tagtgacgat tacaatatca agaccacgta ctttatcaac
     8701 atcatcaaag ctgatttctg ggaagataag ttgttctttc acaccgagag tgtagtttcc
     8761 acgtccatca aacgattttg tcggaacgcc atggaagtca cgtacacgag gaagtgaaac
     8821 gctaaccaat ttgtctagga attcgtacat acgttcgcca cggagggtaa cttttgcacc
     8881 gatcgctaca ccttcacgaa gacggaagcc agcgattgat ttcttagctt tagtaataag
     8941 tggtttttga cctgagataa gtgctaattc agctgcagct ttctctaggt tttttgcgtt
     9001 ggatacagca tcaccaacac ccatattgag aacaattttc tcaacttttg gcacagccat
     9061 aactgatgtg taattgaatt tctctgtcaa cgcaggaatt acttcgttag tatatttttc
     9121 ttttaaacga tttgccatta tgcttctcct ttccttcgtg attaatcaag cacttcgcct
     9181 gattttttgc tgtaacgtac ttttttaccg tcaacaactt tataaccaac acgtccagct
     9241 acaccatttt tatctaatac ttgaacatta gagacatgga taggtgcttc tttttcgacg
     9301 atagctcctt gaggattttc agtgttaggt ttttggtgtt ttttaatcat tccaacacct
     9361 tcaacaataa ctttgttaac ttttgggagt gctttaagaa ctacagcttc agtgcctttg
     9421 tccttgccag caattacgcg aactttgtcg ccttttttta caaacattct ggtttctcct
     9481 attatttctt acgccccgat gggcacccta ggaaaaccta ggggactacg tttgttaatc
     9541 tgagattaaa gtacttctgg tgcaagtgat acgatcttca tgtagccacc ctcacgcaat
     9601 tcgcgtgcaa ctgggccaaa gatacgagtt ccgcgaggag ttttatcatc acggatgatt
     9661 acagcagcat tgtcgtcaaa tttgatgtat gaaccgtctg gacggcgtgc accagttttt
     9721 gtacgaacga taacagcttt aaccacatca ccttttttaa ctgctccacc aggagtagct
     9781 tgttttacag aagcaacgat tacgtcaccg atgttagcga atttacgtcc tgaaccacca
     9841 agtactttga tagtcaagat ctcacgagca ccgctattat cagcaacttt caagcgagtt
     9901 tcttgttgaa tcatttcaat tttctccttt tagtttgatt agataataac agctttttcc
     9961 actacttcca caagacggaa acgttttgta gctgaaagtg ggcgagtttc catgatacga
    10021 acgatatcgc cttctttagc aacgttgttt tcatcatgtg ctttatattt tttagaatag
    10081 ttgatacgtt taccatagac tgggtggtta cgtttagttt caactacaac tgtgattgtt
    10141 ttgtccatct tgtcagacac tacgcgtccg taaagagttt tacgttgatt acgttccatt
    10201 tagaatttct cctttcccaa tctattattt catttctgat tgcacagttt taacacgtgc
    10261 aatttgtttc ttaacttcgt caaggcgagc agtcttttca agttgacctg ctgcagcttg
    10321 gaaacgaaga tcaaacaatt cttttttgag ttcgttttct ttcttagcaa gttcttcttg
    10381 agacaatcca cgaagctctt taacaaaatc tttaatttct tgaagtttca tgtcttctcc
    10441 ttattctgct tcacgtttta cgaatttgca cttaactggt aatttgtggc tagcaagacg
    10501 taatgcttcg cgagcaattt cttcagaaac accagcaatt tcaaacatca ctttaccacg
    10561 tttaactggt gctacccaac cttcaggtgc ccctttacca gaacccatac gtacaccgat
    10621 agcttttgca gtgtatgatt tgtgtgggaa gattttaatc caaactttac caccacgttt
    10681 catgtaacga gtcatcgcaa tacgggcagc ttcgatttga cggtttgtaa tccagtggct
    10741 agttgtggct tgaagtccgt attcaccgaa tgaaacttct ttaccacctt tagcctcacc
    10801 gcgcattttt ccacggaatt cgcgacggtg tttaacacgt ttaggtacta acatttgtta
    10861 tttgcctcct ttagtgtttt tacgagctgg aagaacttct ccacggtaaa tccaaacttt
    10921 aacgccaagt ttaccataag ttgtgtcagc ttcttcccaa gcatagtcga tatcagcgcg
    10981 aagcgtgtga agtggaacag ttccttctga ataaccttca gcacgagcga tatcagcacc
    11041 gttcaaacga ccagaaactt gagttttaat ccctttagct cctgcacgca ttgtacgttg
    11101 gattgcttgt ttttgagcac gacggaaagc aacacgttgc tcaagttgac gagcaatgtt
    11161 ttcaccaaca aggtgagcat ctaaatcagg ttgtttgatt tcgatgatgt tgatgtgtac
    11221 ttgttttcca gttaatttgt taagttgacc acgaagagcg tcaacgtttg cgccaccttt
    11281 accgataacc atacctggtt tagcagtgtg aagtgaaaca ataactttgt ttactgcacg
    11341 ttcaatttca atagttgaaa ctgatgcgtc agctaattct ttattaatga atttacggat
    11401 tgcaagatct tcatgaaggt aatccgcgta ttctttttca gcataccatt tcgcatccca
    11461 gtcacggatg attccgacac gcataccaat tggatgtact ttttgaccca cgattttacc
    11521 tccttatttt tctgatacaa ctacagttac gtgagttgta cgtttgttga ttggtgaagc
    11581 tgaacctttc gcacgtggac ggaaacgttt cattgttggt ccttcgttag cgaatgtttc
    11641 agatactacc aagtttgctt tttccaaacc aaagttgttt tctgcgttag caattgctga
    11701 gttaagagtt ttctcaataa cacgagctgc tttgtttgga gtgaatttta agattgcgat
    11761 tgcgtcagca actttcttac cacggataag atcaagtact aaacgtgttt tacgaggtga
    11821 aacacgcact gtacgagcca ttgctttagc tgaagtaatt tctgccattg tgtcctcccc
    11881 ctattaacga cgtgttttct tgtcgtcagc tgcgtgacct ttgtaagtac gcgttggtgc
    11941 aaattcacca agtttgtgac ctaccatgtc ttcttggatg taaacaggta catgtttacg
    12001 tccgtcataa actgcgattg tatatccgat gaaacttggg aaaatcgttg aacgacgtga
    12061 ccaagttttg attacttttt tcttttcgtc gtttgcttga gcttcaactt ttttcatcaa
    12121 atgctcatcg acgaaaggtc cttttttaag actacgtccc attttgtagt tttctccttt
    12181 aaataatgta ccacagcggc ttgagttcaa gagcaagact ccaaaactct accgagttgg
    12241 cggaataaat agcaaagcta ttgtaacgct atttcagcaa gttttaagca acttactaag
    12301 attatttttc gttacgacga cgaacgataa gtttgtctga tttagctttc ttgttacgag
    12361 ttttaagacc aagcgctggt ttaccccatg gagttgatgg cgctttacgt ccaactggtg
    12421 ctttaccttc accaccaccg tgtgggtgat cgttagggtt cattacagaa ccacgaactg
    12481 ttgggcggat acctttccaa cggctacgtc ctgccttacc aatgttaaca agtgattgtt
    12541 gttcgttacc aacagtaccg atagttgcac ggcatgtacc aagaatcata cgaacttcgc
    12601 ctgattgaag acgaacaaga acgtatttac cttcttgacc aagtacttga gcagaagctc
    12661 cagctgcacg aacaagttct ccacctttac caggtttcaa ctcaatattg tgaacaactg
    12721 taccgacagg aatgtttgct aatggaagtg cgttaccaac tttgatatct gcatctggac
    12781 cagaaacaat acgttgacct acttcaagac ctttaggtgc aatgatgtaa gctttcacac
    12841 cgtcagtgta atgtacaagt gcgatgtttg cagtacggtt tggatcatat tcgattgttt
    12901 taacaactgc ttcaacgcca tctttgttac gtttgaagtc gatcaaacgg taatgacgtt
    12961 tgtgtccacc accttggtga cgaactgtga tgcgaccatt gttgttacga ccagctttgc
    13021 tcttaagaga aacaagcaat gatttctcag gcgtgcttgt tgtgatttcc gcgaaatcca
    13081 aagaagtcat gttacgacgg ccatttgtcg ttggtttata aactttaata cccacgttat
    13141 ttcctcctta gattattcag cttcagctgc gaacaactcg attgctttag aatcagctgt
    13201 aagagtgatg atagcttttt tagtttttga agtgaaacct gtgtaacgac caacgcgttt
    13261 tgcttttggt ttaacgttaa cagtgtttac acttgcaact ttaactccgt caaaagcagc
    13321 ttcaacagct tgtttgatca aaagtttgtg tgcacgagta tcaacttcga aagtgtattt
    13381 gcctgcttca agagcaatca ttgacttctc agtgataact ggttttttga ttacgtcgta
    13441 caaattcatt atgcaagaac ctcctcaatt gtagagattg cttctttagt aacaagaagt
    13501 ttgtctgcgt tcacgatatc aagaacactt gcagttgctg cagttgcaac agttacgttt
    13561 ggaaggttac gtgcagaaag tgctgcaaat tcatttcctt cttcaacaag aacaagtact
    13621 tttgtatcaa tgctaagagc tgaaagcact tttgcaaatt cagcagtttt tggtgctgca
    13681 aatgaaaggc cttctacagc tacaaattta tcttcagcaa cttttgctga gtaaactgat
    13741 ttcaacgcaa ggcgacgaac tttttgtgga agtttgtatc cgtatgaacg gggagttggt
    13801 ccaaagacaa caccaccacc acgccattgt ggtgagcgga tagaaccttg acgagcgcgt
    13861 ccagttcctt tttgacgcca tggtttacgt ccaccacctg atactgctga acggttttta
    13921 accgcatgag taccttggcg aaggctagcg cgttggctaa ttacaacatc aaaaacaact
    13981 gattcgtttg gttcgatacc gaagatagcg tcgtttaatt caactgagct aacttcttta
    14041 ccagtttggt caaatagttt tacgtttgcc attttaactg ttttctcctt tcttattatt
    14101 tagcagcttt aactgctgac ttgatagtga taagagattt cttagcacct ggtacgttac
    14161 ctttaataag gataacgttc ttttctggga taacttgtac aatttcaagg ttttgaactg
    14221 ttacacggtt accacccata cgtcctgcca agcgtttgtt tttgaaaacg cggttaggcg
    14281 caacaggtcc catagaacct gggcgacgat ggtaacgaga accgtgagcc ataggaccac
    14341 gtgattgacc atggcgtttg ataacacctt ggaaaccttt accttttgat gtccctgtga
    14401 cgtcaacaac atcaccagct tcaaattgtt ctacagataa ttctgcacca acttctaagc
    14461 cttcaatgtt tttgaattca cgaatgaagc gcttaggagc tgtgtttgct tttgcaacat
    14521 ggcctttggc aggtttgtta ctcaagactt cacgtttgtc atcaaaacca acctgaactg
    14581 cttcataacc gtctgtttca acagttttaa cttgaagcac aacgtttgga gttgcttcaa
    14641 tgacagtaac agggatgaat tcgcctgatt cagtgaagat ttgagtcatt cccacttttt
    14701 tccctaagat tccttttgtc atgagaaaat atttcctttt cttaataata tttcaaaaag
    14761 ttttttatga gcgttttttg tgctcaaaaa agttttttac gagcgttttt tgtgctcata
    14821 gcaagataca aagagcgtca aactcaaaat aaaaatagaa aatctaacac aaaaacttct
    14881 gtttcgagga agatttatct ttttcatgta gagtttagct cgtgttcaag ttccatcaaa
    14941 cactaactgt acttgcaaaa tctcaccgat taaagtttga tttctacgtt gacaccactt
    15001 ggaagatcaa gtttcatcaa agcatcaaca gttttttgtg ttggattgat gatgtctacc
    15061 aaacgtttgt gtgtacgcat ttcaaattgt tcgcgagaat ctttgtattt gtgagtcgca
    15121 cgaataattg tgtaaagact gcgttcagtt ggaagtggaa ctggtccagc aactgtagca
    15181 cctgtacgtg ttgcagtttc aacgattttt tctgccgctg tatcaagtgt acggtgttcg
    15241 tacgctttca aacggatacg gatttttttg tttgccatct tttctcctct cgtctattta
    15301 cgataatagg ctagctcctc aagaaaaccg acacctgttg cgtggcaatg caaccgagcg
    15361 tgtcgcaacc tcttgtatca ttgctaaagc tgtattttta cagcaccaca atataataac
    15421 agaatcattt agtcattgca agctttttag ggcatttttt caaaaaaact accttcctaa
    15481 tgaaaggtaa tttcttggta ctttttaaat tgtttagaga gaataaacca ccgactggta
    15541 ttatctaaca ctgtcgccat ccagacccct atgagtccat attgccaaac cacaccaatc
    15601 acataaccaa ggccaatgcg aatcacccac attcctatgg ttgttgcata gaagggaagc
    15661 tttgcttttc ccaaaccctg ccagacggct gtataaacta aagtgcctgc cgttgcggga
    15721 gctcctagta aagaaaaaag taaaacaacc atggcggacc tttgagcatc agtattctga
    15781 gtaaatagcg gcaagagact aggacctagc agataggtca aggctcccat aaccagcatc
    15841 atgagggtgg ataaaataaa agcttctcta ataatatacc ttatctcagt caccttaccg
    15901 ccccccagtt gcctggccac tagaataatt gttgccgttg ccatggctaa gccaggcatg
    15961 taattaaact gagtcaaggt ttctccaata gcattacctg ccaaggccgt tgtcccaaaa
    16021 cgaacaacaa tgccaataat taggacatct cctgctctca tcatcaaacg ttcaccagca
    16081 gcaggtagtg ataaatcaaa aataattttg tccaaaggtc tcatcaatcg tttcgcaacc
    16141 tgtttaatag gaataaactg acaaagtaaa aacacaccca ctaaacgaga taaaaccgtc
    16201 gcccaagcaa caccaagaag accaaatccc catacataga tagacaaggc agaaaaaatg
    16261 gcattgagta cgtttattaa taagctcact tgcataggaa tcttaggata cccttgagct
    16321 cgaacaatag ctcctaagct agttaaaagt cctagactaa caatcatacc gccgacaatg
    16381 gatagatatt gccctccaac caatgttaca gaagcttcag ctcctagcca ttctaacacc
    16441 tgatgatgtc caacaactga taacaaccct aagccgaccg aaaggaggag tgttacttgc
    16501 aagacacctg ccatatagtt taattgtttg ttctgattat tttctccaat acttcgagca
    16561 atcagactag atacagcggc tcccagcgcg ataaagaggg actgataaat actgataatg
    16621 ttgttagcta tggagacacc agagactgct acaagtccaa tttgagccac caggtaatta
    16681 tcaaccattc ccattaacat ctgtaaaata ttttctatca tagaaggcag agctaaagag
    16741 aaaatttttc ttctattata tatcatttat gtcccctaat aaaaaattct tgatcaaacg
    16801 atttttttaa acacagtcac tggactcatt cagttatttt gttccattaa tgaattcata
    16861 aagtctacct taatagcaga gtcccaagat acacgacact tagtgttaca gtaacaattg
    16921 accacatgcc aatccttaac atttacctaa cgtttataac taagaagacg aagaacaaaa
    16981 ggactccaaa cgaatttttt ctattatata atatattctt catttagcaa ctttcaatgt
    17041 atcaaaatag cactctcgag tcaaaaatgt tttctgatac tcgagagtgc tattttattt
    17101 ggaatcaagt ttaagcaact taggcttatc tcatatttaa tacatttgta tgtttagctg
    17161 atgaaatcta agaccttgcg cccttggatt aaaccacgtt ccatttcatc aaagacttct
    17221 ggagcagtgt caactggaac tttttcgaca accggagcaa ccagaccttc tgcaccaaag
    17281 gcaaaggctt cttccaagtc tttacgtgtc cctacaagtg atccgaccac tttgatacca
    17341 tctaaaacag tttttacgat agacagttcc atgtattctg atggaagacc tacggctaca
    17401 actgttccac cggcacggac actatcaatt gcttggttaa aagcaacttt agaaacagcc
    17461 gttaccacaa caccatgtgc tccaccggtt ttttcttgaa tatagcctgg aacatcttca
    17521 atttctttgc cgttaactaa aatatctgcg ccaacttctt tagctaactc aagtttatca
    17581 ttattaatgt ccactgcgac aacatgtgca ttaaagactt ttttagcgta ttgaacagcc
    17641 agatttccaa ggccacctgc cccaaagata acaatccatt ggcctggagc agcacctgcc
    17701 tctttaatag ctttataagt tgttacacca gcacaagtaa tggaagaagc ttgagccggg
    17761 tctaaacctt ctggaacttt aactgcataa tcagctgtta ctactgcata ttcactcatt
    17821 cctccatcaa cactgtatcc agcattttta acactgcgac aaagtgtctc acgtcctgta
    17881 gtacagtatt cgcaatgacc acatccttca aagaaccatg caatagagac acgatcacca
    17941 acctttaagg atgttacgcc ttctccaatt tcttctacta taccaatacc ttcgtgcccc
    18001 aaaatacgtc ctggtacctg accaaaatcg ccatgtgcaa cgtgtaaatc agtatgacaa
    18061 acgccacagt attctacttt aacaagcgct tcaccatgac caacatttgg aagatcatgc
    18121 tcaacaactt cgacacctgt actagcttga ttaacaacta cggctttcat atcagtcctc
    18181 catctaataa cgacttattt gtaatcggtt acaaataaat tgtacatctt ttcacaatta
    18241 aagtcaacag aaacctgaaa agtttctgaa acttaactgg ttaatcttat agctgtttgc
    18301 ctatctgcaa aatacttaac tacattttct gacactggat ataaatgata tatttagaaa
    18361 ataaaatgat ttcttatgac tttaatcata agaaatcatt tcctggtatc aacattaaaa
    18421 atcacttacg tcgtcctgga cgttcagcat aaccatagta agcatcttca ataatttctt
    18481 gcatgtgatc aaccattgga agacgtgggt tagcaggtga acattggtct tcataagcaa
    18541 ggtaagccaa ttcacgagaa tgttctttcc attcattttc gtcgatacct tgagctttga
    18601 agttcatttg aataccaact cggcaaccca gatcataaac agctttcgca taagattcca
    18661 ctgcttcttc tggtgtagaa gctggaagcc ctaacaattt cgcaatgtct tggtattttt
    18721 catccgcacg gtagtaatta tacttaggcc atgtcgccgt tttagctgga cgagttccat
    18781 tatagcggat aacatacggc aaaagaattg catttgtacg tccgtgtacg gtatggaatt
    18841 gtgcgccaat tttatgcgcc attgagtgag aaatacctaa gaatgcattg gcgaaagcca
    18901 tacctgccat agttgacgca ttgtgcatct tttcacgtgc ttcaaaatcg gctattttaa
    18961 ctgatttttc aagattatca aaaacaattt taatggcttg aagagctaaa ccatccgtaa
    19021 agtcattggc catttgtgac acgtaagctt cggttgcgtg tgtcaaaacg tccataccag
    19081 tatcagcagc aataaagcct ggaacagtca ataccaaagc tgggtcaaca atggctacag
    19141 ttggtgttaa tgagtaatca gcgattggat atttacgatt atttgctttg tcagagataa
    19201 cggcaaatgg tgtcacctct gaacctgttc cagaagttgt tgggatagca acaaattttg
    19261 ttttcttacc aagttctggg aatttgaacg cacgtttgcg gatatccatg aatttttgaa
    19321 cgaggtcatg gaaatcaact tctggttgct cgtagaagag ccacataact ttggctgcat
    19381 ccataggaga tcccccgcca agggcaataa tggtgtctgg tttgaaggta cgcattaatt
    19441 cagtaccttt cataacagtt gtaatgtctg gatctggttc tacttcagca aagatctgat
    19501 aaacaacttt attgcgacga agatcaagtt gttcaatgat acgatccaag aaaccaagct
    19561 caaccatagc gtgatctgta acaatcatga cacgttcaac gtcacgacat ttttgcaaat
    19621 attgaattga atcacgttcg aagtatgttt ttgaaggaac tttaaaccat tgcatattat
    19681 ttctacgtct tcctactttt ttgatgttca agagatttat agcactaaca ttatcaccaa
    19741 ctgcgttacg tccatacgaa ccacaaccaa gagtcaatga tggcaagaaa gcattgtaaa
    19801 catccccgat accaccaaat gtagaaggtg aattccagat gacgcggata gcacggattc
    19861 tagttccaaa ttcttttgcc aactctgcat ctgctgtatg aatggcagct gagtgaccga
    19921 gtccattaaa ttcaaccatt tgacgagctt tttcaacacc gtcttcacgt gattcagatt
    19981 tcaaaacagc aatgactgga gataattttt cacgtgttaa aggctcattt tctgaaactt
    20041 ctttacattc tgcagcaaga atattggttc cttctggaac agtaaatcca gcttgttctg
    20101 caatccaagt agctggtttt cctacaatat ttggatttaa ttttgcacca gcacagtttt
    20161 tgctatttgc tttggcacca aaacagaatt cttcaagaag ggctttttct tttttattta
    20221 caaaataagt atggtaagat ttgaattctg ctacaaaatc gtcgtagatt tctttatcga
    20281 tgataacagc ttgttcagag gcacagacca taccattatc aaatgattta gacatcacaa
    20341 tatcatgagc ggcttgacgg atattagctg atttttcaac ataagctgga acgttaccag
    20401 cacctacccc aagggcaggt ttcccacatg agtatgccgc tttaaccatc gcatttccac
    20461 ctgtcgcaag gatagtggca atgccgtcat gattcatcaa agcatttgtt gcttcaagtg
    20521 atggtgtttc aacccactgc acacaatttt caggagcacc agcggcaata gcagcatcac
    20581 gaacaatacg agcggcatgt gctgatgatt cttgtgctga tgggtgaaag gcaaaaataa
    20641 ttggattacg tgtttttaac gagatcaaag atttaaagat agctgttgaa gttgggttag
    20701 ttgtgggtgt aatcccacag ataacaccca ccggctcagc aattaatgtc aaacctgtca
    20761 catcatcttc ttcaataatt cctactgttt tttgatggcg catgttatta accacatgct
    20821 cacaagcaaa tagatttttt gttgctttat cttcaaagac accgcgacct gtttctttat
    20881 aagcatgttt agcaagttca ccgtgagcat ccaaggcagc aacagaagct ttggctacga
    20941 tataatcaac ttgttcttgg tcaagcttac gcatctcttc aagagcagct aaaccttttt
    21001 gaacaagagc attaattgtc acagaaacag aggttgtttc aacagtatta tgtccttcag
    21061 tcatttgaga ctcctttatc ttgttaaaag tttcacaatt ttgtttacaa aattattata
    21121 gcactttttg aaattttgta aatcatttca caataaattt gtgaagtttt tttcaaatta
    21181 tttctgaaat cct
//
LOCUS       DATXIG010000024        20999 bp    DNA     linear   BCT 28-DEC-2023
DEFINITION  TPA_asm: Streptococcus pyogenes strain MGAS11113
            SAMN08555799-rid19682583.denovo.024, whole genome shotgun sequence.
ACCESSION   DATXIG010000024 DATXIG010000000
VERSION     DATXIG010000024.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN08555799
            Sequence Read Archive: SRR6740495
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pyogenes
  ORGANISM  Streptococcus pyogenes
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 20999)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 20999)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-OCT-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-OCT-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001918058.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001918058.2
            Genome Coverage       :: 368x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/12/2023 11:23:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 1,806
            CDSs (total)                      :: 1,769
            Genes (coding)                    :: 1,722
            CDSs (with protein)               :: 1,722
            Genes (RNA)                       :: 37
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1, 1 (5S, 23S)
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 47
            CDSs (without protein)            :: 47
            Pseudo Genes (ambiguous residues) :: 0 of 47
            Pseudo Genes (frameshifted)       :: 30 of 47
            Pseudo Genes (incomplete)         :: 20 of 47
            Pseudo Genes (internal stop)      :: 9 of 47
            Pseudo Genes (multiple problems)  :: 11 of 47
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..20999
                     /organism="Streptococcus pyogenes"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN08555799-rid19682583.denovo.024"
                     /strain="MGAS11113"
                     /serotype="Emm28"
                     /isolation_source="normally sterile site"
                     /host="Homo sapiens"
                     /db_xref="taxon:1314"
                     /country="Finland"
                     /collection_date="2002"
                     /collected_by="National Public Health Laboratory"
     gene            49..462
                     /locus_tag="VC122_000973"
     CDS             49..462
                     /locus_tag="VC122_000973"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011017766.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HEP5640908.1"
                     /translation="MKLNAIHHVAIIVSDYHLSKDFYVNKLGFEIIRENYRSDKHDYK
                     LDLSCGRIELEIFGKVTSDPNYQAPPKRVSQPEFKSEACGLRHLAFRVTNIESYVDDL
                     KSLGIPVEPIRHDDYTGEKMTFFFDPDGLPLELHE"
     gene            1076..1510
                     /locus_tag="VC122_000974"
     CDS             1076..1510
                     /locus_tag="VC122_000974"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002984633.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEP5640909.1"
                     /translation="MTKKNHGVFWGLLLGSAAASIAYLSLSSSKKDQLLKDSAKKIDD
                     LNAYLQDKSKQVLDAVSEKVQESKDAVEVYGGIAAETVEEKWQDTKDAVEVYGGIATE
                     TVEESLGQVKEKVEGIGEATSQTIQSKMEKLTSDKTDADDEK"
     gene            complement(1560..2471)
                     /locus_tag="VC122_000975"
     CDS             complement(1560..2471)
                     /locus_tag="VC122_000975"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002995001.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diacylglycerol kinase family lipid kinase"
                     /protein_id="HEP5640910.1"
                     /translation="MKTVRIFYNPNSGKKENQLARQVKDYFCQHGFSEDSVEVITPKD
                     ADQAFQLAKQAAKDKIDLVIPLGGDGTLNKIIGGIYEGGAHCLIGLVPSGTVNNFAKA
                     MHIPLQITEALDTILTGQIMQVDICKANQQYMISSLTLGLLADIAADVTAEEKRRFGP
                     LAFLKDSIRILKRNRSYAISLISHNHRIHLKTKFLLITMTNTIAGFPSFSPGAQADDG
                     YFQVYTMKKVSFFKFLWHINDFKQGEFSKAEEISHFQANTLSLLPQAKKQAILPRTRI
                     DGDKSDYLPIQLDIIPKAISIIVPTED"
     gene            complement(2588..3238)
                     /locus_tag="VC122_000976"
     CDS             complement(2588..3238)
                     /locus_tag="VC122_000976"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014407582.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hemolysin III family protein"
                     /protein_id="HEP5640911.1"
                     /translation="MNQTFKQSLPLSFSEEVANSVTHAIGAFAMLILLPISASYAYQT
                     YDLKAAIGISIFVISLFLMFLSSTIYHSMAYGSVHKYILRIIDHSMIYIAIAGSYTPV
                     ALSLVSGWLGYIIIVLQWGITLFGILYKIFAKRINEKFSLMLYIVMGWLVVFILPVII
                     QKTSLAFGLLMLFGGLSYTIGAVFYAKKRPYFHMIWHLFILLASALQFIAITFFML"
     gene            complement(3235..3675)
                     /locus_tag="VC122_000977"
     CDS             complement(3235..3675)
                     /locus_tag="VC122_000977"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003050852.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1836 domain-containing protein"
                     /protein_id="HEP5640912.1"
                     /translation="MIFPSLPYWKDLPDLDLYLDQVLLYVNQCTDFSEVSDNKSLTAS
                     MINNYVKHGYVTKPIKKKYQKQQLARLIAISLFKTVFPIQDISRVLEELQAQADSESL
                     YNTFVTCWNQKAPIEEDIPPIVQVACQTVKDYHKTIYLLQEVIQ"
     gene            3894..4742
                     /gene="ylqF"
                     /locus_tag="VC122_000978"
     CDS             3894..4742
                     /gene="ylqF"
                     /locus_tag="VC122_000978"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014407584.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome biogenesis GTPase YlqF"
                     /protein_id="HEP5640913.1"
                     /translation="MATIQWFPGHMSKARRQVQENVKHVDFVTILVDARLPLSSQNPM
                     LTKIVGDKPKLMILNKADLADATRTKEWKAYYESQGIKTLAINSKEQSTVKKVTEAAK
                     ELMADKIQRLRERGIQKETLRTMIIGIPNAGKSTLMNRLAGKKIAVVGNKPGVTKGQQ
                     WLKSNKELEILDTPGILWPKFEDELVGLKLALTGAIKDQLLPMDEVTIFGLNYFKTHY
                     PSRLTKRFKNIPLEEEAPEIIMTLTRQLGFKDDYDRFYTLFVKEVRDGKLGQYTLDQV
                     GDMDVD"
     gene            4732..5523
                     /locus_tag="VC122_000979"
     CDS             4732..5523
                     /locus_tag="VC122_000979"
                     /EC_number="3.1.26.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002995007.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease HII"
                     /protein_id="HEP5640914.1"
                     /translation="MSTSIKAIKESLEAVTSLSDPLFQELATDARLGVQKALKSRQKA
                     IQADLAEEERLEAMLSYEKALYKEGYQAIAGIDEVGRGPLAGPVVAACVILPQHCKIK
                     GLNDSKKIPKSKHETIYQAVKEKALAIGIGIIDNQLIDEVNIYEATKLAMLEAIKQLE
                     GQLTQPDYLLIDAMTLDIAISQQSILKGDANSLSIAAASIVAKVTRDQMMANYDRIFP
                     GYGFAKNAGYGTKEHLQGLKAYGITPIHRKSFEPVKSMCCDSTNP"
     gene            5588..6424
                     /gene="dprA"
                     /locus_tag="VC122_000980"
                     /pseudo
     CDS             5588..6424
                     /gene="dprA"
                     /locus_tag="VC122_000980"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010922314.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-protecting protein DprA"
     gene            6531..8660
                     /gene="topA"
                     /locus_tag="VC122_000981"
     CDS             6531..8660
                     /gene="topA"
                     /locus_tag="VC122_000981"
                     /EC_number="5.6.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002989768.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I DNA topoisomerase"
                     /protein_id="HEP5640915.1"
                     /translation="MGTKTTTKPKTGTKKSTTKKKSTAKKNLVIVESPAKAKTIEKYL
                     GRSYKVVASVGHIRDLKKSSMSIDFDNNYEPQYINIRGKGSLINSLKKEAKAAKKVYL
                     ASDPDREGEAISWHLSHILGLDPQDNNRVVFNEITKDAVKHAFVEPRQIDMDLVDSQQ
                     ARRVLDRIVGYSISPILWKKVKKGLSAGRVQSVALKLIIDRENDIKAFVPKEYWSIDG
                     LFKKGTKKFQATFYGINGKKTKLDNNNDVKEVLAKLTNEDFLVSKVDKKERRRNAPLP
                     YTTSSLQQDAANKINFRTRKTMMVAQQLYEGIHLGENGTQGLITYMRTDSTRISPVAQ
                     NDAAQFIINRFGANYSKHGNRVKNTSGAQDAHEAIRPSSVNHTPDSIAKYLNKDQLKL
                     YTLIWNRFVASQMTAAVFDTVKVNLEQNGVIFVANGSQMKFDGYMAVYNDSDKNKMLP
                     EMAEGETVKKISTSPEQHFTQPPARYSEATLIKTLEENGVGRPSTYAPTLEVIQRRYY
                     VKLSAKRFEPTELGEIVNKLIVEFFPDIVDVAFTAEMEGKLDQVEIGEEQWQHVIDQF
                     YQPFVKELNKAESEIEKIQIKDEPAGFDCDVCGHPMVIKLGRFGKFYACSNFPECRNT
                     KAITKEIGVTCPVCHKGQVIERKTKKNRIFYGCDQYPDCEFISWDLPIGRACPKSGDY
                     LIEKKVRGGKQVMCSNETCDYKEEKIK"
     gene            complement(8735..9619)
                     /locus_tag="VC122_000982"
                     /pseudo
     CDS             complement(8735..9619)
                     /locus_tag="VC122_000982"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000354842.1"
                     /note="frameshifted; internal stop; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
     gene            9632..10177
                     /locus_tag="VC122_000983"
                     /pseudo
     CDS             9632..10177
                     /locus_tag="VC122_000983"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015631865.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIC"
     gene            10192..11184
                     /locus_tag="VC122_000984"
     CDS             10192..11184
                     /locus_tag="VC122_000984"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010922317.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-lactate dehydrogenase"
                     /protein_id="HEP5640916.1"
                     /translation="MKLKLYNVRGEEAVLAKKWADDNGIEISLTESPLTPETVKEAEG
                     FDGIANAQIGPLDDAIYPLLREMGIKQIAQRSAGVDMYNLDLATENDIIITNVPSYSP
                     ESIAEFTVTIALNLIRHVELIRENVKKQNFTWGLPIRGRVLGDMTVAIIGTGRIGLAT
                     AKIFKGFGCKVVGYDIYQSDAAKAVLDYKESVEEAIKDADFVSLHMPPTAENTHLFNA
                     DLFKSFKKGAILMNMARGALIETQDLLDALDAGLLSGAGIDTYEFEGPYIPKNFEGQE
                     ITDSLFKALINHPKVIYTPHAAYYTDEAVKNLVEGALNATVEIIKTGTTTTRVN"
     gene            11289..11954
                     /locus_tag="VC122_000985"
     CDS             11289..11954
                     /locus_tag="VC122_000985"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002989760.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SatD family protein"
                     /protein_id="HEP5640917.1"
                     /translation="MTYIALIGDIIQSKQLTDRSKVQKTLAAYLDDLNKTFAPYIISK
                     LSLTLGDEFQGLFQVDTPIFHLIDLINHHMDIPIRFGVGVGSILTDINPDISIGADGP
                     AYWHAREAIRYIHQKNDYGNTTLAFRTGHHNQDDVLNSLLAAGDAIKANWRASQWEIF
                     DTLLDLGIYEEYFDQQRLGKQLSLSSSALSKRLKSSHVKIYLRTRQSALNCLKQIKEE
                     TDD"
     gene            11947..12663
                     /locus_tag="VC122_000986"
     CDS             11947..12663
                     /locus_tag="VC122_000986"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015057577.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3307 domain-containing protein"
                     /protein_id="HEP5640918.1"
                     /translation="MINGVSHYLAQTPTLTLFLICHVLSDYQLQSQQVADLKEKHLTY
                     LGYHLIGVSIPLICLTLIIPQAWLMSLLVMISHALIDWLKPKMANSLKWKREWIFLLD
                     QCLHIAISSFAGLRLAGVTLPNWLPISILMTVLFILLITKPTNIVFKLFFSKYQPDQG
                     EKMDTIIGAGATIGILERIVIGVCMFMGQFASIGLVFTAKSIARYNKISESPAFAEYY
                     LIGSLFSILSVFIAAWICFF"
     gene            12809..14155
                     /gene="trmFO"
                     /locus_tag="VC122_000987"
     CDS             12809..14155
                     /gene="trmFO"
                     /locus_tag="VC122_000987"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014635468.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methylenetetrahydrofolate--tRNA-(uracil(54)-
                     C(5))-methyltransferase (FADH(2)-oxidizing) TrmFO"
                     /protein_id="HEP5640919.1"
                     /translation="MSQSTATYINVIGAGLAGSEAAYQIAKRGIPVKLYEMRGVKATP
                     QHKTTNFAELVCSNSFRGDSLTNAVGLLKEEMRRLDSIIMRNGEANRVPAGGAMAVDR
                     EGYAKSVTAELENHPLIEVIRDEITEIPDDAITVIATGPLTSDALAEKIHALNGGDGF
                     YFYDAAAPIIDKSTIDMSKVYLKSRYDKGEAAYLNCPMTKEEFMAFHDALTNAEEAPL
                     NAFEKEKYFEGCMPIEVMAKRGIKTMLYGPMKPVGLEYPDDYTGPRDGEFKTPYAVVQ
                     LRQDNAAGSLYNIVGFQTHLKWGEQKRVFQMIPGLENAEFVRYGVMHRNSYMDSPNLL
                     TETFQSRNNPNLFFAGQMTGVEGYVESAASGLVAGTNAARLFKREEALVFPQTTAIGS
                     LPHYVTHADSKHFQPMNVNFGIIKELEGPRIRDKKERYEAIASRALADLDTCLASL"
     gene            14308..15714
                     /locus_tag="VC122_000988"
     CDS             14308..15714
                     /locus_tag="VC122_000988"
                     /EC_number="6.4.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011527649.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate carboxylase subunit B"
                     /protein_id="HEP5640920.1"
                     /translation="MIQFGGFSMQQQVAITETVLRDGHQSLMATRLSIEDMLPVLTIL
                     DKIGYYSLECWGGATFDACIRFLNEDPWERLRTLKKGLPNTRLQMLLRGQNLLGYRHY
                     ADDIVDKFISLSAQNGIDVFRIFDALNDPRNIQQALRAVKKTGKEAQLCIAYTTSPVH
                     TLNYYLSLVKELVEMGADSICIKDMAGILTPKAAKELVSGIKAMTNLPLIVHTHATSG
                     ISQMTYLAAVEAGADRIDTALSPFSEGTSQPATESMYLALKEAGYDITLDETLLEQAA
                     NHLRQVRQKYLADGILDPSLLFPDPRTLQYQVPGGMLSNMLSQLKQANAESKLEEVLA
                     EVPRVRKDLGYPPLVTPLSQMVGTQAAMNVILGKPYQMVSKEIKQYLAGDYGKTPAPV
                     NEDLKRSQIGSAPVTTNRPADQLSPEFEVLKAEVADLAQTDEDVLTYALFPSVAKPFL
                     TTKYQTDDVIKVTAFIKA"
     gene            15757..16071
                     /locus_tag="VC122_000989"
     CDS             15757..16071
                     /locus_tag="VC122_000989"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002995020.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEP5640921.1"
                     /translation="MTYSFIDILQVTLVSLFLVLTCLTGIMGLMMLSGKLLSQEKKTL
                     SQQASSIPQEQEADKLSGEALETLLAKDELARIAVMTSLAYASQEESGKRFEVIAVER
                     KS"
     gene            16071..16421
                     /locus_tag="VC122_000990"
     CDS             16071..16421
                     /locus_tag="VC122_000990"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003057661.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="biotin/lipoyl-binding protein"
                     /protein_id="HEP5640922.1"
                     /translation="MKRYEVTVNGQVYEVSLLELADGETVQVSRPAAPATEKEMNANA
                     AGGGLQVKAPMSGTVLSIFATEGKAVKKGEAVLVLEAMKMENEILAPADGLVSKIHVV
                     ANQTVESEQVLISF"
     gene            16431..17561
                     /locus_tag="VC122_000991"
     CDS             16431..17561
                     /locus_tag="VC122_000991"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002995027.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sodium ion-translocating decarboxylase subunit
                     beta"
                     /protein_id="HEP5640923.1"
                     /translation="MLDVLNQMVQSSGLAHLTVNNLIMICLACFFLYLGIKKEYEPYL
                     MVPIAFGILLVNLPMAGLMDHPANGNPGGLLYYLYKGTSLGIYPPLIFLCLGASTDFG
                     PLIANPKTILLGGAAQVGIFLAFFLAIMLGMTPQEAASVGIIGGADGPTAIYVTTKLA
                     PDLLSTIALAAYSYMALVPIIQPPIIKLLTTKAERQVKMTQARTVSQKEKIIFPIMVT
                     IFVSLLVPSATTLVGCLMLGNLVREIKIVPKIVENLQQVVMFCITIILGLTVGAKANG
                     DLFLSATTLKIIALGLIAFAAGTAGGVLMGKVMYYLSGNKVNPMIGAAGVSAVPMAAR
                     VVQKVGQEEDPSNFLLMHAMGPNVAGVIGSAIASGALLAFFG"
     gene            complement(17762..18646)
                     /gene="citG"
                     /locus_tag="VC122_000992"
     CDS             complement(17762..18646)
                     /gene="citG"
                     /locus_tag="VC122_000992"
                     /EC_number="2.4.2.52"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011888866.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="triphosphoribosyl-dephospho-CoA synthase CitG"
                     /protein_id="HEP5640924.1"
                     /translation="MTKAVLTSISQLALKALLYEVSLSPKPGLVDRFDNGAHDDMSFM
                     TFIDSMIALSPFFQAYIETGFAYAKEEPLLLFNRLRQLGQKAEETMFCATQGINTHKG
                     LNFSMALLLGATGAYLARTPHLMTDLGCFSKEDTLAICRLVKPMTAHLIQTDLGHLNT
                     KKEFTYGEQLFVTYGIKGPRGEASEGFTTLTDHALPYFRQMISQNDPETSQLRLLVYL
                     MSIVEDGNLIHRGGIEAWKGVKADMRLLLQQDLSTTDLRLALSSYNQCLINQHLSPGG
                     AADLLALTFYFAFLEKLL"
     gene            complement(18639..19334)
                     /locus_tag="VC122_000993"
     CDS             complement(18639..19334)
                     /locus_tag="VC122_000993"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010922324.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HEP5640925.1"
                     /translation="MNPIIEAVKQNLDLSRNIPLKIAFYNALKTTIILRQIPVGSRIN
                     EKEFSIALNISRTPIRYALGLLSEEHLVEHIPKKGIIVKGVSIKDACEIFEIRKALET
                     LATVQAMHLMTEEDFKVMHNLLEDCETFIAEDDTNRILDNFNAFNNLIYSYSQMVRLK
                     EIVTELQAYLVYFRKISISSVERRKRALSEHWMIYRGMKNKDHEQITLITHEHLNSSL
                     EFILKEMRPEQND"
     gene            complement(19492..20895)
                     /locus_tag="VC122_000994"
     CDS             complement(19492..20895)
                     /locus_tag="VC122_000994"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003088270.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CitMHS family transporter"
                     /protein_id="HEP5640926.1"
                     /translation="MLLTMLAYAMIIVFMYVVMKKKMTPFTALVMIPLIMTIAVILTG
                     SADFNADAKFVAFVGDGGIAKDLTAIGPMVMYGINNTAKTGIMLLFAILFFSVMLDAG
                     LFDPITEKMIRFAKGDPMKVLIATAVVAAAVSLNGDGTTTTLICCSAFLPIYKKLDMK
                     IMNLGVLIILQNTIMNLLPWGGPTARAMSVLGVGPEILGYLAPGMILSLLYVICWVAP
                     SMGRKERARLGVIDLSEEDMRQLTDITDPDTLFIRRPKNFVFNAILTIGLITWLVAGS
                     FNKSIAMAPLLLFAVGTCIALMVNYPVLKDQSKRIGDNAGDAVQVVILVFAAGIFMGL
                     FQGSGMASALAQSFATIIPKQLAGFWGLVIALVSAPGTFFISNDGFYYGILPVLAEAG
                     AEYGFSNMAMALASLMGQAFHLLSPLVAFIYLLLRLTGLDMGEWQKEAAKYALIIFVI
                     FVVTIIAMGQMPLYIPQ"
BASE COUNT         6662 a         3856 c         4220 g         6261 t
ORIGIN      
        1 catagagaaa atttatctca tcctactttt tttgaaagga gaaatatgat gaaactaaat
       61 gccattcatc acgttgctat tattgtatct gattatcatc tgtcaaaaga tttttatgtg
      121 aataaactcg gatttgaaat tatcagagaa aattatcgtt ctgacaagca tgattacaaa
      181 ttagatttat cttgtggtcg tattgaattg gagatttttg gtaaggtgac aagtgacccc
      241 aactatcaag ctcctcctaa acgtgtaagt caaccagaat ttaaaagtga ggcctgtggt
      301 ttgaggcacc ttgcttttcg tgttaccaac attgagagtt atgtagatga tctaaagagc
      361 ttaggaattc cagtggagcc tattaggcat gatgattaca caggtgagaa aatgactttc
      421 ttttttgatc cggatggttt gcctttagaa ttacatgaat agacttatta ggttaacgac
      481 tggatcgtgc tattaaactc gttaaaaata gttatttgtt gtatcttctt ttgcttgatt
      541 aatctctttt gatcgttaaa tgatttggtt aactttcaaa gtgaagagca atttttggag
      601 taatcttgtc gaagtttttt aatagaataa ctataggaaa agcaaaatat ttgacgataa
      661 actcttacag tgttataatg acaatataag taaataactg tgttgtcact ttgttgaaat
      721 gttttcgtat taatgttttt ttatctatta cgatagataa ttttacttca atttttgatt
      781 gaaaatacta aaataagtga tgtggtcact ttgacaacca tttattggtc cttttagata
      841 agacaatttt gtggctcttc agttgaaagg atgtgttgtt gatgttagag gaaacaatca
      901 tgttaaatta actcaggctt aaagaccaaa aaaggagtga agtctattat gaaaaaaaga
      961 aattttaaac ttacagaaat gttacaattg gttttattag tggttcctgc tgtaaaactt
     1021 gctaaaaaaa tgtttaacga tagtaaaaca ccgtctaaaa gaaagtaggt ctacaatgac
     1081 taaaaaaaat catggcgttt tttgggggtt gttgcttgga tcagcagcag ctagcattgc
     1141 ttacttaagt ttatcgtctt ccaaaaaaga tcaactcttg aaagatagtg ctaaaaaaat
     1201 agatgatctc aacgcttact tgcaagataa aagtaagcaa gttctggatg cagtatcgga
     1261 aaaagtacaa gaatccaaag atgctgttga agtttatggt ggtattgccg cagaaaccgt
     1321 tgaagaaaag tggcaagata ccaaagatgc tgttgaagtt tacggtggta ttgccacaga
     1381 aacagttgaa gaatctttgg gacaggttaa agaaaaggta gaaggaattg gcgaagctac
     1441 tagccaaact atccaatcta aaatggaaaa actcaccagc gataagacag atgctgatga
     1501 tgaaaaatag gttttaaaat acctttaaga taagacaaaa agtgccatta ggcacttttt
     1561 taatcttctg ttggaacaat aatcgaaatg gcttttggaa tgatatctaa ttgaatgggc
     1621 agatagtcac ttttatcccc atcaatcctg gtacgtggta atatcgcttg ttttttagct
     1681 tgtggtagta agctaagcgt attggcttga aaatgactga tttcctctgc tttggaaaac
     1741 tcaccctgtt taaaatcatt aatatgccat aaaaatttaa agaaagagac ttttttcatg
     1801 gtataaactt ggaaatagcc atcgtcagct tgagcaccag gagaaaaaga aggaaaacca
     1861 gcaatggtat ttgtcatcgt gattaagaga aacttcgttt tcaggtgaat acgatggttg
     1921 tggctaatta aagagatggc atagctgcga tttcgtttca gtattcgaat actatctttt
     1981 aaaaatgcta gaggaccaaa gcgtcgtttt tcctcagccg ttacgtcggc agcaatatca
     2041 gccagtaatc ctaaggttaa actggaaatc atgtattgct ggttagcttt gcaaatatca
     2101 acttgcatga tttgtccagt caagatagta tccaacgcct cagtaatttg taaagggata
     2161 tgcatagcct tggcaaaatt attgactgtc cctgagggca ctaagcctat taaacaatga
     2221 gctcctcctt catagatgcc accgatgatt ttatttagtg tcccatcgcc accaagaggg
     2281 ataaccaagt caatcttgtc cttagcagct tgttttgcta attgaaaagc ctggtcagca
     2341 tctttagggg taataacttc tacagaatct tcggaaaaac catgttggca aaaatagtct
     2401 ttgacttgcc tagcaagttg gttttctttc tttccagaat taggattgta aaaaatccga
     2461 acggttttca tcaggtctct cctttgacag cttattactt gctatccatt ataccacttc
     2521 taagaattga tgtcttacat caaatcatca cattaagcct ctcaatcagt ctgagcatct
     2581 atttgtctta aagcataaag aaagtgatag cgataaattg gagggcagag gccaataaaa
     2641 taaagagatg ccaaatcatg tggaagtagg gtcttttttt ggcataaaat accgctccaa
     2701 tggtataaga caagccacca aataacatga gaagaccaaa agcaagacta gttttttgaa
     2761 tgatgactgg caagataaac acaacgagcc agcccataac gatataaagc atcaaactga
     2821 atttttcatt gatacgtttg gcaaaaattt tgtataaaat cccaaaaagc gtaatgcccc
     2881 actgcaaaac aataatgata tagcctagcc aaccagaaac aagggataag gcaacaggtg
     2941 tataacttcc tgcgatagca atgtaaatca tactgtgatc aataatgcgt agaatgtatt
     3001 tgtgaaccga accataagcc atggagtggt aaatcgtaga ggacaaaaac atcaaaaaga
     3061 gactgatgac aaagatggag ataccaatag cggctttaag gtcataagtt tgataagcat
     3121 agctggctga aataggaagt agaatgagca tagcaaatgc tccgatagcg tgtgtcacgc
     3181 tgtttgctac ttcctcacta aaagataagg gtaaactttg tttaaaggtt tggttcattg
     3241 gatcacctcc tgtaataaat aaatagtttt gtggtaatct ttgaccgttt gacaggcaac
     3301 ctgcacaatc ggtggaatgt cctcctcaat gggagccttc tgattccaac aggttacaaa
     3361 agtattgtaa agactttcag aatcagcctg tgcctgaagt tcctccaata cacgactaat
     3421 atcttgaata ggaaataccg ttttaaacaa actgatggca attaagcgcg ctagttgttg
     3481 cttttggtat tttttcttga taggtttagt cacataccca tgcttgacgt aattattaat
     3541 catagatgcc gttagtgatt tattatctga cacttctgaa aaatcagtgc actggttgac
     3601 ataaagtaag acttgatcca aatataagtc taaatcaggg aggtccttcc aataaggtaa
     3661 tgatggaaaa atcatagttt tgtctctcta tctagttttg ataactagat tatacgatta
     3721 atgaaaactt attgcaagca aaaactgtaa agacttaaaa gaggaaagta gaaatggcga
     3781 agtgaagcta atcatgctat aatgttaaag aattgattgg gatagatgcc ttctgtcgca
     3841 atcagactat tttatcacga agaagatgat gctacgaaag gaatttaaac tctatggcta
     3901 ctattcaatg gtttcctggg cacatgtcca aagccagaag acaggttcag gaaaatgtaa
     3961 agcatgttga ctttgtaact attttagtgg atgcacgctt accactttct agtcaaaatc
     4021 ccatgcttac aaaaattgtt ggtgataaac ctaaattaat gattttaaat aaagctgact
     4081 tggcagatgc aactcgaact aaagagtgga aggcttatta tgaaagtcaa ggtattaaaa
     4141 cattagccat caattccaaa gaacaatcaa cggttaaaaa agtaacagaa gcagccaagg
     4201 aactcatggc tgataaaatt caacgcttgc gagagcgggg cattcaaaaa gaaaccttgc
     4261 gaaccatgat tattggcatt ccaaatgctg gtaaatctac cttaatgaat cgcttggctg
     4321 gtaagaaaat tgctgtagtt ggcaataaac ctggtgtgac taaaggacaa caatggttga
     4381 agtccaataa ggaattggaa attctagaca ctcctggtat tttatggcca aaatttgagg
     4441 acgaattggt gggcttaaaa ttagctttaa caggtgctat caaggaccaa ttactaccaa
     4501 tggatgaggt cactattttt ggtttgaatt actttaaaac acattatcct agtcgcctta
     4561 ctaagcgttt taagaatatt cctcttgagg aagaagctcc tgaaatcatt atgaccttga
     4621 cacgccaact gggatttaaa gatgattacg accgtttcta cactttattc gtcaaggaag
     4681 ttcgtgatgg taaattaggt caatacacct tagatcaggt gggagatatg gatgtcgact
     4741 agtattaaag ctattaaaga aagcctggaa gctgttacta gcctctcaga ccccctcttt
     4801 caagaattgg caactgacgc taggttaggt gtccaaaaag cgctaaaaag tcgacaaaag
     4861 gctattcagg ccgatttagc agaggaagaa cgattagaag ccatgctttc ttatgaaaaa
     4921 gcgctttata aagaaggcta ccaagccatt gcaggtattg atgaggtggg acgtggcccc
     4981 ctagcaggtc ccgttgtcgc agcatgtgtc atcttacctc aacattgcaa aattaaaggt
     5041 ctgaatgatt ctaaaaaaat ccctaaatct aagcatgaga ccatttatca ggcagtgaaa
     5101 gaaaaggctt tggctatcgg tatcggtatt attgacaatc agcttattga tgaggtcaat
     5161 atttatgaag caaccaaact agctatgcta gaagccatta aacagttgga gggccaactc
     5221 acacaaccag attatctctt gattgatgcc atgacattgg atattgctat ttcgcagcag
     5281 tctattctta aaggcgatgc caattccttg tctattgcag cagcatcaat tgtagctaag
     5341 gtcaccagag atcagatgat ggctaactat gatcgcattt ttcctggtta tggcttcgct
     5401 aaaaatgcag gctatggcac caaagaacat ttacagggat taaaagctta cggcataacg
     5461 cctatccatc gtaaaagttt tgaacctgtt aaatccatgt gctgcgattc aactaatcct
     5521 taaaaagttc tgtttttagc agaatttttt tcttttttac gaataaatga agtaaggagg
     5581 taacttagtg aatcattttg aactttataa gctaaagaaa gctggactga ctaataaaaa
     5641 cattctcaat attcttgact accaagaaca ccaggaaaaa tcgttgtcac ttcgagatat
     5701 ggccgttgtt tctggttgta agcatccgtc tcactttata gaagcctata agcagttaga
     5761 tattcaaaaa ttaaaaatag aatttaaaca atttcctagt atttctatct tagataagca
     5821 ttacccaatg gcattgaaag aaatatacaa tccacctgtc ctcttgtttt ttcagggaaa
     5881 tttagacctt cttgagaaac ctaaattagc cattgtcggc tccagacgct caagcgatac
     5941 cggagtaaag tctgtccgta aaattcttaa agaactcggg aaccgttttg tgattgttag
     6001 cggacttgct cgaggtatcg acactagtgc tcatttagcc tgccttaaaa atgggggaca
     6061 aactatcgct attattggaa caggattgga tcgcttttac cctaaagaaa atagggagtt
     6121 gcaaactttc ttagggaaaa atcatcttgt gctaacagaa tacggtccag gagaagaagc
     6181 tttatcttat cactttccag aacggaatcg tattatcgca ggccttagcc gaggtattct
     6241 tgtcgttgaa gcaaaaaatc gttcaggttc cttgattact tgtcaaattg ggatagaaga
     6301 aggctgagac atttttgctg tcccaggaaa cattttggac gggaaatccg aaggttgcct
     6361 tcagttaatt aaagagggag caacatgcgt cacatcgggc atggatatcc tttcagagta
     6421 ctaaaaaagt taaagtttct tcctatatag cattttttta ttgacagttt cttaaaaatg
     6481 ggatattatg ctataagctt taaacaaact gttataggaa gtgtgtcgaa ttgggaacaa
     6541 aaactacaac taagccaaaa acgggtacta aaaagtccac cactaaaaag aaatcaacag
     6601 cgaaaaaaaa tctcgttatt gttgaatcac ctgccaaagc taagactatt gaaaaatatc
     6661 ttggacgcag ttataaagtt gttgcttcgg taggacatat tcgtgactta aaaaaatcgt
     6721 ctatgtctat tgattttgat aataattatg agccacaata cattaatatc cgtggtaagg
     6781 gatcccttat caattcttta aaaaaagaag ccaaagctgc taaaaaagtt tatttagcta
     6841 gtgacccgga tcgtgaggga gaggctattt cttggcattt gtcacatatc ttaggacttg
     6901 atcctcaaga taataaccga gtggttttta atgaaattac gaaagatgca gttaaacatg
     6961 cctttgtgga gcctcgtcag attgatatgg acttggtaga ttctcaacaa gcacgtcgtg
     7021 ttttggaccg tattgtggga tattccattt caccaattct ttggaaaaag gtcaaaaaag
     7081 gactttcagc aggacgtgtc cagtcggttg ctttaaaact catcattgac cgtgaaaatg
     7141 acatcaaagc atttgttcca aaggaatact ggtctattga tggtcttttt aaaaaaggaa
     7201 ctaaaaagtt tcaagcgact ttttacggta ttaatggcaa aaaaacaaag cttgataata
     7261 acaacgatgt caaagaggta ttagcaaagc taacaaatga agattttctt gtcagcaagg
     7321 ttgacaagaa agagcgacgt cgtaatgctc ccttacctta cacaacatca tctcttcaac
     7381 aagatgctgc taataaaatc aatttcagaa cgcgtaaaac catgatggta gcccaacaac
     7441 tgtatgaggg gattcattta ggagaaaatg gaactcaagg tttaattacc tatatgcgta
     7501 cggattctac tcgtatcagt ccagtagcac aaaacgatgc tgctcaattt attatcaatc
     7561 gctttggagc taattattca aaacatggta atcgtgtcaa aaataccagt ggtgctcaag
     7621 atgctcacga ggcgattcgt ccatctagtg ttaatcatac gccagactct attgccaaat
     7681 accttaacaa ggatcagtta aaactttata cgcttatttg gaatcgtttt gtggctagcc
     7741 aaatgacagc agctgttttt gataccgtaa aggtgaattt ggagcaaaat ggcgtgattt
     7801 ttgttgccaa tggtagccaa atgaagtttg atggttacat ggctgtttac aatgattccg
     7861 ataaaaacaa aatgttacct gagatggcag aaggagaaac ggttaaaaaa atatcgacct
     7921 ctccagaaca gcactttacg cagccaccag ctcgttattc tgaagcaact ctgattaaaa
     7981 cgctagaaga aaatggtgtt ggacgtccat caacttatgc cccaacctta gaagtgattc
     8041 aacgcaggta ctatgttaaa ttatctgcta agcgctttga gccaactgag cttggggaaa
     8101 ttgtcaacaa gcttattgtc gaatttttcc cagatattgt tgatgttgcc tttactgccg
     8161 agatggaagg taagctagat caagttgaaa tcggagaaga acaatggcaa catgtcatcg
     8221 atcaattcta tcaacccttt gtcaaagaat tgaataaggc tgagtctgaa attgaaaaga
     8281 ttcaaatcaa agatgagcca gctggttttg actgtgatgt ttgtggccac ccaatggtca
     8341 ttaagttagg acgttttggg aaattttacg cgtgcagcaa cttccctgag tgtcgtaata
     8401 caaaagccat cactaaagaa atcggtgtta cttgtccggt ttgtcataaa ggacaagtta
     8461 tcgagcgtaa aaccaaaaaa aataggatat tttatggttg tgatcagtat ccagattgtg
     8521 agtttatctc gtgggattta cctattggac gtgcttgccc taagtcaggt gactatctta
     8581 ttgagaaaaa agttcgtggt ggcaaacaag taatgtgtag caatgagacc tgtgattaca
     8641 aggaagaaaa aattaaataa aaaaagaatt ggaactcctt gtcttgttcc aattcttttt
     8701 ttatttacaa aggtattttt aactacaaaa atcgctagtt cgtttttaaa aggcttaaaa
     8761 attcctcaat aactggactg aaactcgcat catctcggta agcaatccca atagggtaaa
     8821 aattattttt atcttgcaaa ggaatccaaa ctaagttatc tacctgatta agactctcaa
     8881 aatcactcgg gaggatagtc aaggtattta aatcgtgcaa actatgaata agaacttccc
     8941 aatcattgtg tttaaagaca atatctggtt cataaccgag agctcggcat tttctaggaa
     9001 gcatttcgcc taacatgtag tgatcgttta aagaagcaat cttatagcct tttaaatctt
     9061 taagttttaa ctgaggatgt tgcgccaaag ggtgctgttt gaggagaacg atactgacct
     9121 tatagccttt tgtggacgtt tgtaatagct caatggtaat atccttttga atacttagaa
     9181 aggagaggag accaatatcg atttttcctt gggacaataa ttcttataat ttgcgagaac
     9241 catcctgtat aagcgataat tcaacatttg agtgagtagc cataaagatt gaaatttgtt
     9301 tcatgaattg tatagcaaac agtgatatta attcaacacg aatcatctct ttttttcttg
     9361 atttaattgc tgaatatccg caacaatttg attaaagtca tccacaaggt ctttgccttt
     9421 gtgataaaga atatctccag cttcggttag tagtagctgt caatcacttt gtgtaaaaag
     9481 cgtcgtgttc aaactaggac tctagccttt taattgattg agaaagagta tgttgcgtta
     9541 caaacaaact tttagcagcg tgggaatgat tttttgtttc agcaactgca ataaaatagg
     9601 ttaattatct aatatccata gtagatcctt catgattggt cagggaattg cgtctctact
     9661 tggtttacaa cctattttga tgtctctcct tattgccatg attttctgct tccttattgt
     9721 ttctccaatt acaacggtag gaattgctct tgcgattaac ttatctggta ttggttcagg
     9781 tgccgcaagc tttggacttt gcttagcagg ttgggctgtt aattcaaaag gaacttcact
     9841 tgctcacgtc ttgagatcac cgaaaatttc aatggccaac gttttatcaa aaccaaaaat
     9901 catgttgcca atgttatgtt ctgcagctgt acttggtgtt attggtgcta tctttaatat
     9961 tcaaggaaca cctgctagtg caggctttgg aattagtggt ttgatcggac ctatcaatgc
    10021 ccttaattta gctaaaggcg gatggtctcc agtaaatatt ctacttatca ttatcatttt
    10081 tgtaggggca cccattgtgc ttaatatgat ctttaactac ctctttatta aggtgcttaa
    10141 agtgattgat ccaatggatt acaagcttaa tatctaagaa aggaactttt gatgaaatta
    10201 aaattatata atgttagagg cgaagaagca gtcttagcaa aaaaatgggc tgatgacaat
    10261 gggattgaga tttccttgac agagtctccc ttaacccctg aaacggttaa agaagctgaa
    10321 ggctttgacg ggattgccaa tgcccaaatt ggtcctttag atgatgcaat ctatcctctc
    10381 ttaagggaaa tggggattaa acaaatcgct cagcgcagtg ctggtgtcga tatgtataac
    10441 cttgatttgg ccaccgaaaa tgacattatt atcacaaatg ttcctagcta ttctccagaa
    10501 tccattgcag aatttacggt tactattgcc cttaatttaa ttcgtcatgt ggagttgatt
    10561 cgtgaaaatg ttaaaaaaca aaatttcact tggggactcc ctatccgcgg tcgtgtttta
    10621 ggtgatatga cagttgcaat tatcggaact ggacgtattg gtctagctac tgctaaaatc
    10681 tttaaaggtt ttggctgcaa ggtcgttgga tatgatattt accaaagcga tgctgccaaa
    10741 gctgtcttgg actataagga atctgtagaa gaagcgatta aagatgctga ttttgtttcc
    10801 ttacacatgc caccaactgc agagaacaca caccttttca atgctgattt atttaaatca
    10861 ttcaaaaaag gggctatttt gatgaatatg gcgcgtggtg cccttattga gacgcaagat
    10921 ttactggatg ctttggatgc aggcttattg agcggagctg gtattgacac ttacgaattt
    10981 gaaggacctt atattcctaa gaattttgaa ggtcaagaaa ttacagattc actatttaaa
    11041 gctttgatta accatcctaa ggttatttat acgcctcatg cagcttatta tactgatgaa
    11101 gccgttaaaa acctagttga aggtgccctt aatgctacag tagagattat caaaactggg
    11161 acaacgacaa ctcgtgttaa ctaacaactg atttgacgtc cattataaag gaataattcc
    11221 aaaaattcct ttataatgga cttttttgca ttagtccgtt ataatagact gtaagaaagg
    11281 gggctcttat gacatatata gcgcttattg gagatattat ccagtccaaa caattgacgg
    11341 atcgttccaa agttcaaaaa actttagcag cttatttaga cgacctcaac aaaacctttg
    11401 ctccttatat tatttctaaa ttatccctaa cgttgggaga tgaatttcaa ggactttttc
    11461 aggtggatac tcctattttc catctgattg atttgattaa tcatcacatg gatatcccta
    11521 ttcgatttgg agtaggagta ggttctattc taacagacat caatcctgat attagtattg
    11581 gggctgatgg acctgcttat tggcatgctc gtgaggctat tcgttatatt catcaaaaaa
    11641 atgattatgg taatactaca ttagcttttc ggactggtca tcacaaccag gatgatgtat
    11701 taaacagttt actagcagct ggagatgcta ttaaagccaa ctggcgagct agtcaatggg
    11761 aaatttttga cacgctttta gatttgggca tttatgagga atactttgat caacaacgac
    11821 taggaaagca attatctctt agctctagcg ctttgtcaaa acgtctaaaa agtagtcatg
    11881 ttaaaattta tctgagaaca cggcagagtg cgctaaactg tctcaaacaa atcaaggagg
    11941 aaactgatga ttaatggtgt ctctcactac ctagctcaaa cccctacact taccctgttt
    12001 ctcatttgtc atgtgttatc agactaccag ttacaaagtc agcaagtagc tgatttaaaa
    12061 gaaaaacatt tgacttattt aggttaccac ttgattggag ttagcattcc cctaatttgt
    12121 ttaaccctca ttatcccaca agcatggttg atgagcctat tggttatgat aagtcacgcg
    12181 cttattgact ggctaaagcc aaagatggct aactctctaa aatggaaaag agagtggatt
    12241 tttctgctgg atcaatgctt acatatcgct attagttcat ttgctgggct aaggttagca
    12301 ggggtgacct tgcctaattg gcttccgata tctattttga tgaccgtttt atttatccta
    12361 ctcattacca aaccaactaa tattgtcttt aaactctttt tcagcaaata ccagcctgac
    12421 caaggagaaa aaatggacac gatcatagga gcgggggcta ccataggaat tctagagaga
    12481 attgttattg gggtgtgtat gttcatgggg caatttgctt caataggtct ggtctttacc
    12541 gctaaatcta ttgctcgcta taataaaatt tcggaaagtc cagcttttgc agaatactac
    12601 ctaattggtt ctttatttag tattctcagt gtttttatcg cagcatggat ttgtttcttc
    12661 tagttgaaaa gggttcccgt agaaagaggc tacacatagc ctcttttgct tttttcccct
    12721 ttctgttata ataagtcagg taactacatt agaaagattt gtgggtctgt catctaagag
    12781 atatcattcc cgaagaggta ttagtgactt gtctcaatca actgcaactt atattaatgt
    12841 tattggagct gggctagctg gttctgaagc tgcctatcag attgctaagc gcggtatccc
    12901 cgttaaattg tatgaaatgc gtggtgtcaa agcaacaccg caacataaaa ccactaattt
    12961 tgccgaattg gtctgttcca actcatttcg tggtgatagc ttaaccaatg cagtcggtct
    13021 tctcaaagaa gaaatgcgtc gcttggattc cattattatg cgtaatggtg aagctaatcg
    13081 tgtacctgct ggaggagcaa tggctgttga ccgtgagggg tatgcaaaga gtgtcactgc
    13141 agagttggaa aatcatcctc tcattgaggt cattcgtgat gaaatcacag aaatccctga
    13201 cgatgccatt acggttatcg cgacgggtcc attgacttca gatgccttgg cagaaaaaat
    13261 tcacgcgcta aatggtggcg acggattcta tttttacgat gcagcagcgc ctatcattga
    13321 taaatctacc attgatatga gcaaggttta ccttaaatct cgctacgata aaggcgaagc
    13381 tgcttacctc aactgcccta tgaccaagga agaattcatg gctttccatg atgcactaac
    13441 aaacgcagaa gaagcaccgc tgaatgcctt tgaaaaagaa aagtattttg aaggctgtat
    13501 gccgattgaa gttatggcta aacgtggcat taaaaccatg ctttatggac ctatgaaacc
    13561 cgttggattg gaatatccag atgactatac aggtcctcgc gatggagaat ttaaaacgcc
    13621 atatgccgtc gtgcaattgc gtcaagataa tgcagctgga agcctttata atatcgttgg
    13681 tttccaaacg cacctcaaat ggggtgagca aaagcgtgtt ttccaaatga ttccagggct
    13741 tgaaaatgct gagtttgtcc gctacggcgt catgcaccgc aattcctata tggattcacc
    13801 aaatctttta accgaaacct tccaatctcg taacaatcca aatcttttct tcgcaggtca
    13861 gatgactgga gttgaaggtt atgtcgaatc agctgcttca ggtttagtag caggaaccaa
    13921 tgctgctcgt ttgttcaaaa gagaagaagc gcttgttttt cctcagacaa cagctattgg
    13981 tagcttgcct cattatgtga ctcatgccga cagtaagcat ttccaaccaa tgaacgtcaa
    14041 ctttggcatt atcaaagagt tagaaggccc acgcattcgt gacaaaaaag aacgttatga
    14101 agctattgct agtcgtgctt tggcagattt agacacctgc ttagcgtcgc tttaacacta
    14161 aaaaagagtt ccttttcagt tgagttgctg aagaggactt ttttccttgc tgatttgagc
    14221 gctagctatg caaaagaatg cacaaaaaat acatgaatag actatggtca ctagaagcaa
    14281 tattatataa tgaatagtac tcattagttg attcagtttg gaggttttag catgcagcag
    14341 caagtcgcaa taacagaaac agttttacgg gacggtcatc aaagtttgat ggcaacgcgt
    14401 ttatctattg aggatatgct tcctgtcctt accatcttag acaagattgg ttattattcc
    14461 ctagagtgtt ggggaggagc aacctttgat gcctgtattc gttttttgaa cgaagaccct
    14521 tgggaaagac tgcgcacgtt aaaaaaaggc ttacctaata cccgtttaca aatgttatta
    14581 agaggccaaa accttttagg ctaccgtcat tatgcagatg acatcgttga caaattcatt
    14641 agtttatcag ctcaaaatgg gattgatgtt ttccgcattt ttgatgccct aaatgaccca
    14701 cgaaatatcc agcaagcctt aagagctgtg aaaaagacgg gtaaagaggc tcaactgtgt
    14761 attgcctata caactagccc agtgcacacg cttaactatt acctttcttt agtgaaagaa
    14821 ttggttgaga tgggagctga ttctatctgc atcaaagata tggctggtat tctaactccc
    14881 aaagcggcta aagagttagt atctggtatc aaagccatga ctaacttacc tcttattgtg
    14941 catacccatg caactagtgg catttctcag atgacctatc tagcagcagt agaagctggt
    15001 gctgatagaa ttgatactgc tttgtcacct tttagcgaag gcacttcaca acctgctaca
    15061 gaatccatgt atttagcctt aaaagaagca ggttatgata tcacattaga tgaaacgcta
    15121 ctggaacagg ctgctaatca ccttcgacaa gtccgtcaaa agtacttagc agatggcatt
    15181 ctggatccca gtcttctctt tccagaccca aggacactac aataccaagt tcctggaggc
    15241 atgttatcta atatgctatc tcagctgaaa caagccaatg ccgaatcaaa attagaagaa
    15301 gttttagcag aagttcctcg ggtaagaaaa gacttgggtt atcctccctt agtaacaccg
    15361 cttagccaaa tggtcgggac tcaagctgct atgaatgtga ttttaggaaa accttaccag
    15421 atggtgtcta aggaaattaa acaatacttg gcaggcgact atggcaaaac accagcacca
    15481 gtcaacgaag acttaaaacg aagtcaaatt ggctcagctc ctgtcaccac aaatcgtcct
    15541 gcagatcaac taagtcctga attcgaggtt ttaaaagcag aagtagcaga tttagcccaa
    15601 acggacgaag atgtgctgac ttatgccctc ttcccaagcg tagcaaaacc gtttttaaca
    15661 accaaatacc aaactgatga cgtcattaaa gtgactgcct ttatcaaagc ataataggct
    15721 atcttaatca gatagaaaca aaaatatgga gatcaaatga cctattcctt tattgatatc
    15781 ttacaagtta ccttagtatc cttgttctta gtgttaacct gtttaacagg gattatggga
    15841 ttaatgatgc taagtggtaa attattgtca caagaaaaaa aaactctctc tcaacaagct
    15901 tccagcattc ctcaagagca agaagctgac aagttgagtg gagaagctct cgaaactctt
    15961 ttagccaaag acgaattggc tagaattgct gttatgacta gtcttgctta tgctagccaa
    16021 gaagagtcag gaaaacggtt tgaagttatt gccgtggaaa ggaaatccta atgaaaagat
    16081 atgaagtgac tgtcaatgga caggtttatg aagtgagttt acttgaacta gctgatggag
    16141 aaactgttca agttagccgg ccagcagcac ctgctactga gaaagaaatg aatgccaacg
    16201 ctgctggcgg tggcctacag gtaaaggctc caatgtctgg aacagtcttg tctatctttg
    16261 ctacagaagg aaaagctgtt aaaaaaggag aagctgttct ggtcttagag gccatgaaaa
    16321 tggagaatga aattctagca cctgcagatg gtctggtttc aaaaattcat gttgtggcta
    16381 accaaacggt cgagtctgaa caagttttga tttcattcta ggaggaaacc atgctagacg
    16441 tactcaatca aatggtccaa tcgtcaggat tggctcatct aactgttaat aatcttatca
    16501 tgatttgctt ggcctgtttc tttttatacc ttggtatcaa aaaggaatat gagccttacc
    16561 ttatggtacc aatcgccttt gggattttac tcgttaattt gcctatggcg ggattgatgg
    16621 accaccctgc caatggtaat cctggtggcc ttctttatta cctatataag ggaactagcc
    16681 tagggattta cccaccttta atttttcttt gtttgggggc atcaactgac tttggtccct
    16741 tgattgccaa tcctaaaacc attttactag gaggcgcagc acaagttggg attttcttag
    16801 cctttttcct tgctattatg ttaggcatga cacctcaaga agcggcttct gtcgggatta
    16861 ttggtggtgc cgatggacca acagctattt atgtgacgac aaagttggcg cctgatttac
    16921 tctctactat cgccctagca gcttattctt acatggctct ggtacctatt attcaacctc
    16981 ctatcattaa actcttgact accaaggcgg aacgccaagt gaaaatgact caagcaagaa
    17041 ctgtttcaca aaaagaaaaa atcattttcc cgattatggt aacaatcttt gtcagccttt
    17101 tggttccaag tgctacaacc ttggttggct gtttgatgct gggaaatctg gtcagagaaa
    17161 ttaaaattgt tcctaaaatt gtagagaatc tccaacaagt ggttatgttt tgcattacta
    17221 tcattttagg attaaccgtt ggtgctaagg ccaatggaga tctcttttta tcagccacaa
    17281 ccttgaaaat tattgccctt gggctaatcg cctttgctgc tggtacagca gggggtgtct
    17341 tgatggggaa ggtcatgtac tatttatctg gtaacaaagt gaaccctatg ataggagcag
    17401 caggtgtttc agctgttccg atggcagcgc gagtggtcca aaaggtcgga caagaagaag
    17461 atccaagtaa tttcttactc atgcacgcca tgggacccaa tgtagctggc gttattggct
    17521 cagccattgc ttcaggtgcc ttgctggcct tctttggcta attgtgaaaa aggattagta
    17581 aattaatctt aataaatcat tatcaggagt tggaagcaat ctagctcctt ttttagtaga
    17641 aagctcgagt gataaactat tccaaaataa ttataaaaaa agactgtcct gattgtacgg
    17701 actatatcaa ttgactttta tagtctagct gaaatagcag tgcaaaacta agacactcgt
    17761 tttaaagtag tttttctaaa aaagcaaaat aaaaggtcag agccaataaa tctgccgcgc
    17821 ccccagggct taaatgctga ttaatgaggc attgattgta ggaggacaaa gccaatctta
    17881 ggtcagttgt tgataaatct tgttgcagga gtagtcgcat atctgcttta acgcctttcc
    17941 atgcttcaat ccctccacga tgaatcagat ttccatcttc aacaatagac atcaaataaa
    18001 caagaagacg aagctgactg gtttcggggt cattttgact tatcatctga cgaaagtagg
    18061 gcaaggcatg gtctgttaag gtcgtgaatc cctcgcttgc ctcaccccga ggtcctttga
    18121 tgccataagt tacaaagagt tgctccccat aagtgaactc ttttttagtg ttcaaatgac
    18181 caaggtctgt ttgaatcaaa tgtgccgtca taggttttac taaacgacag atagctagag
    18241 tatcttcctt gctaaaacac cctaaatcag tcattaagtg aggtgttctt gctagatagg
    18301 ccccagttgc accaagaagt aaagccatgg aaaaattcag gcctttatga gtgttaattc
    18361 cctgagtagc acaaaacatg gtttcctcgg ccttttgacc tagttgtcta agacggttaa
    18421 acagaagaag tggctcttct tttgcataag caaaacctgt ttcgatataa gcctgaaaaa
    18481 aaggagagag tgctatcata ctgtcgataa aggtcataaa ggacatgtca tcatgggcac
    18541 cgttatcaaa tcgatcaact aaaccaggtt ttggagatag tgagacttca taaagcaaag
    18601 cttttaaggc cagttgacta atacttgtta gaactgcctt agtcattttg ctcaggcctc
    18661 atttctttta agataaattc aagtgagcta ttcaagtgtt catgtgtaat caacgtaatt
    18721 tgctcatgat ctttgttctt catacctcgg taaatcatcc agtgttcaga tagagctcgt
    18781 ttacggcgtt caactgaaga aatcgaaatt tttctaaagt agaccaaata agcttgcaat
    18841 tctgtcacaa tttcttttaa gcggaccatt tggctatagc tgtaaatcaa attgttaaag
    18901 gcattaaaat tatccaaaat acgattggtg tcatcctctg cgataaaggt ttcacagtct
    18961 tccaacaagt tatgcatgac tttaaaatct tcttctgtca ttaaatgcat cgcttgcact
    19021 gttgcgagag tctctagagc ttttcgaatt tcaaaaattt cacaggcatc cttgatacta
    19081 actcctttga caataatgcc ttttttagga atgtgctcta caaggtgttc ttcagacaat
    19141 aatcccaaag cgtagcgaat aggtgtcctg ctaatattaa gcgcaatgga aaactctttt
    19201 tcattaatgc ggcttccaac aggaatttgt cgcaaaataa tggtcgtttt aagagcgtta
    19261 taaaaggcta ttttcaaagg gatatttctt gataggtcaa gattttgttt aacggcttcg
    19321 ataataggat tcatgtcatc tccttaatgc tagtgtcctc actttattat acttaaattt
    19381 gtgaaacatt ccaataggac tagatgagtt ctgaaaaatt aaagccatgc tcacaaaagg
    19441 acatgacttt aatcatctca taaggaataa ggtagtagtg ttatcgttgg tttattgtgg
    19501 aatgtaaagt ggcatttgcc ccatggcaat aatggttacc acaaagataa caaagatgat
    19561 aagggcatat ttagcagcct ctttttgcca ttcccccatg tcaagacctg tcaagcgaag
    19621 aagaagataa atgaaggcaa ctaatggact aagtaagtgg aaggcttgtc ccatgaggga
    19681 agcaagtgcc atagccatgt tactgaaacc atattcagca ccagcttctg caagaacagg
    19741 caagatacca tagtaaaaac catcattgga gataaagaag gtaccaggtg cagaaactaa
    19801 ggcaataaca agaccccaga aacctgccag ttgttttgga ataatggttg caaaactttg
    19861 tgcaagagcg ctagccatac cagaaccttg gaaaagaccc ataaagatac cagcagcaaa
    19921 gactaagata accacttgaa ccgcatcacc agcattgtca ccaatgcgtt ttgattgatc
    19981 tttaagtact gggtaattga ccatcaaggc gatacaagtt cccaccgcaa aaagaagaag
    20041 cggtgccata gcaatagatt tgttgaaaga gccagcaact aaccaagtga ttaatccaat
    20101 ggttaggata gcattgaaaa caaagttttt aggacgacga ataaaaaggg tatctgggtc
    20161 agtaatgtct gtgagttgac gcatgtcttc ttcagacaag tcaataacac caagtcttgc
    20221 acgttcttta cgccccatgc ttggagcaac ccaacaaatc acataaagaa gagataaaat
    20281 catacccggt gcaagatagc caagaatttc aggaccgaca ccaagaacag acatcgcacg
    20341 agcagtaggg ccaccccatg gcagtaagtt cataatagta ttttgaagaa taatcaagac
    20401 acctaggttc atgattttca tgtccaattt tttatagata ggtaagaaag cagagcaaca
    20461 aattaaagta gtggttgttc catcaccatt aagagatact gcagcagcaa cgacagctgt
    20521 tgcgataagc accttcattg gatcaccttt agcaaagcga atcatctttt cagtaatcgg
    20581 atcaaacaat cctgcatcta acatgacaga gaagaacaaa atggcaaata gcaacatgat
    20641 acctgtttta gcagtattgt tgataccata cataaccatt ggtccgatag ctgttaggtc
    20701 tttagcaatt ccaccgtcac caacaaaggc aacaaatttg gcatctgcat tgaagtcagc
    20761 tgaaccagtc agtataacag caatcgtcat gattaatgga atcatgacca aagcagtaaa
    20821 aggggtcatt tttttcttca ttaccacgta cataaagaca ataatcatgg cataagccag
    20881 cattgttaat aacatcattg ttatctcctt tttcaagtta tgatttcagt atagatgaaa
    20941 gcgcttataa aataaatact tctttgtgca gaaagacaca aaaaaaacaa tttgaaaat
//
LOCUS       DATXIG010000025        20685 bp    DNA     linear   BCT 28-DEC-2023
DEFINITION  TPA_asm: Streptococcus pyogenes strain MGAS11113
            SAMN08555799-rid19682583.denovo.025, whole genome shotgun sequence.
ACCESSION   DATXIG010000025 DATXIG010000000
VERSION     DATXIG010000025.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN08555799
            Sequence Read Archive: SRR6740495
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pyogenes
  ORGANISM  Streptococcus pyogenes
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 20685)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 20685)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-OCT-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-OCT-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001918058.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001918058.2
            Genome Coverage       :: 368x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/12/2023 11:23:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 1,806
            CDSs (total)                      :: 1,769
            Genes (coding)                    :: 1,722
            CDSs (with protein)               :: 1,722
            Genes (RNA)                       :: 37
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1, 1 (5S, 23S)
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 47
            CDSs (without protein)            :: 47
            Pseudo Genes (ambiguous residues) :: 0 of 47
            Pseudo Genes (frameshifted)       :: 30 of 47
            Pseudo Genes (incomplete)         :: 20 of 47
            Pseudo Genes (internal stop)      :: 9 of 47
            Pseudo Genes (multiple problems)  :: 11 of 47
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..20685
                     /organism="Streptococcus pyogenes"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN08555799-rid19682583.denovo.025"
                     /strain="MGAS11113"
                     /serotype="Emm28"
                     /isolation_source="normally sterile site"
                     /host="Homo sapiens"
                     /db_xref="taxon:1314"
                     /country="Finland"
                     /collection_date="2002"
                     /collected_by="National Public Health Laboratory"
     gene            110..1093
                     /gene="guaC"
                     /locus_tag="VC122_000995"
     CDS             110..1093
                     /gene="guaC"
                     /locus_tag="VC122_000995"
                     /EC_number="1.7.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007891361.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GMP reductase"
                     /protein_id="HEP5640927.1"
                     /translation="MFNDIPVFDYEDIQLIPNKCIITSRSQADTSVTLGKYQFKLPVI
                     PANMQTIIDETIAERLAKEGYFYIMHRFDEDSRKPFIKRMHEQGLIASISVGVKACEY
                     EFVTSLKEDAPEFITIDIAHGHANSVIDMIKHIKTELPETFVIAGNVGTPEAVRELEN
                     AGADATKVGIGPGKVCITKVKTGFGTGGWQLAALRWCAKAARKPIIADGGIRTHGDIA
                     KSIRFGASMVMIGSLFAGHIESPGKTVEVDGETFKEYYGSASEYQKGEHKNVEGKKIL
                     LPTKGHLSDTLTEMQQDLQSSISYAGGKDLDSLRHVDYVIVKNSIWNGDSI"
     regulatory      1216..1311
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00167"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="purine riboswitch; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /bound_moiety="guanine and/or adenine"
                     /db_xref="RFAM:RF00167"
     gene            1398..1979
                     /locus_tag="VC122_000996"
     CDS             1398..1979
                     /locus_tag="VC122_000996"
                     /EC_number="2.4.2.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003054011.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="xanthine phosphoribosyltransferase"
                     /protein_id="HEP5640928.1"
                     /translation="MQLLEERILTDGNILGENILKVDNFLTHQVDYRLMKAIGKVFAQ
                     KYAEAGITKVVTIEASGIAPAVYAAEAMDVPMIFAKKHKNITMTEGILTAEVYSFTKQ
                     VTSTVSIAGKFLSKEDKVLIIDDFLANGQAAKGLIEIIGQAGAQVVGVGIVIEKSFQD
                     GRRLIEDMGIEVTSLARIKNFENGNLNFLEADA"
     gene            1979..3262
                     /locus_tag="VC122_000997"
     CDS             1979..3262
                     /locus_tag="VC122_000997"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002984674.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="purine permease"
                     /protein_id="HEP5640929.1"
                     /translation="MTHSTKQEHSHSQSAVLGLQHVLSMYAGSILVPIMIAGALGYSA
                     RELTYLISTDIFMCGVATFLQLKLTKHTGVGLPVVLGCAFQSVAPLSIIGAQQGSGAM
                     FGALIASGIYVILVAGIFSKIARFFPPIVTGSVITVIGLSLVGVAMGNMGDNVKEPTA
                     QSMMLSLLTIVIILLVQKFTKGFVKSISILIGLVVGTLVSAMMGLVDTTPVVEASWIH
                     VLTPFYFGMPTFEITSIVMMCIIATVSMVESTGVYLALSDLTNDQLDEKRLRNGYRSE
                     GIAVFLGGLFNTFPYTGFSQNVGLVQISGIKTRRPIYYAAGILVVIGLLPKFGAMAQM
                     IPSPVLGGAMLVLFGMVALQGMQMLNRVDFQKNEYNFIIAAVSISAGLGFNGTNLFAS
                     LPETAQMFLTNGIVIATLTSVVLNLVLNGKDKQDE"
     gene            complement(3326..4264)
                     /locus_tag="VC122_000998"
     CDS             complement(3326..4264)
                     /locus_tag="VC122_000998"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002994961.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD:protein FMN transferase"
                     /protein_id="HEP5640930.1"
                     /translation="MFVTQQLKLMGTVIDIQIESDKACQQLSRVIDLLYTYKNRFSAN
                     DSNSELMAINQAAGVKPLSVHSDLFNLIQIGKAHSLSTPSNLNIAIGPLVQAWRIGFE
                     DARVPSHNLISQQLALTDPRQVLIDDKKQTVFLQQVGMALDLGALAKGYITDKIMAYL
                     IEDGIDSALINLGGNVRVHGPNPKSPDKTFRIGIQKPDAKRGQHLGVIKVNNHSVVTS
                     GIYERQFTAKGKQYHHILDRQTGYPIETDMLSLTIMAPSSFYCDIWATRLFGLDSSMI
                     ITLLNTFDNIEGLLVTRKHHVLMSNGLRHYFQPYYH"
     gene            complement(4317..4502)
                     /locus_tag="VC122_000999"
     CDS             complement(4317..4502)
                     /locus_tag="VC122_000999"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002984671.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-oxalocrotonate tautomerase"
                     /protein_id="HEP5640931.1"
                     /translation="MPFVTIDLFEGRSQEQKNQLAREVTEVVSRIAKAPKENIHVFIN
                     DMPEGTYYPQGEMKQKS"
     gene            4640..5209
                     /locus_tag="VC122_001000"
     CDS             4640..5209
                     /locus_tag="VC122_001000"
                     /EC_number="2.7.1.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000068112.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thymidine kinase"
                     /protein_id="HEP5640932.1"
                     /translation="MAQLYYKYGTMNSGKTIEILKVAHNYEEQGKPVVIMTSALDTRD
                     GFGIVSSRIGMRREAIPISNDMDIFTFIAQLEEKPYCVLIDESQFLSKQNVYDLARVV
                     DELNVPVMAFGLKNDFQNNLFEGSKHLLLLADKIDEIKTICQYCSKKATMVLRTENGK
                     PVYEGDQIQIGGNETYIPVCRKHYFNPEI"
     gene            5244..6323
                     /gene="prfA"
                     /locus_tag="VC122_001001"
     CDS             5244..6323
                     /gene="prfA"
                     /locus_tag="VC122_001001"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010922302.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor 1"
                     /protein_id="HEP5640933.1"
                     /translation="MNIYDQLQAVEDRYEELGELLSDPDVVSDTKRFMELSREEANTR
                     ETVTAYREYKQVIQTISDAEEMIKDASGDPELEEMAKEELKESKAAKEEYEEKLKILL
                     LPKDPNDDKNIILEIRGAAGGDEAALFAGDLLTMYQKYAETQGWRFEVMESSVNGVGG
                     IKEVVAMVSGQSVYSKLKYESGAHRVQRVPVTESQGRVHTSTATVLVMPEVEEVEYDI
                     DPKDLRVDIYHASGAGGQNVNKVATAVRMVHIPTGIKVEMQEERTQQKNRDKAMKIIR
                     ARVADHFAQIAQDEQDAERKSTVGTGDRSERIRTYNFPQNRVTDHRIGLTLQKLDTIL
                     SGKMDEVIDALVMYDQTKKLESLNN"
     gene            6323..7162
                     /gene="prmC"
                     /locus_tag="VC122_001002"
     CDS             6323..7162
                     /gene="prmC"
                     /locus_tag="VC122_001002"
                     /EC_number="2.1.1.297"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002994971.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor N(5)-glutamine
                     methyltransferase"
                     /protein_id="HEP5640934.1"
                     /translation="MNYATLIRTYEDKLEQIDEDRENLSYVFREIKEWSSLDMLIHQN
                     QAVTPEDAVLLEHIFCSLSQHLSPQYITGNAYFRDLKLAVDKRVLIPRPETEELVDMI
                     LAENLDAPLNVLDIGTGSGAIAISLKKERPNWQVTASDISRAALDLAKANADAYQLDI
                     TFIESDVFSLISGTFDIIVSNPPYISYEDKEEVSLNVLQSEPHLALFAKENGYAIYRK
                     IIEQADNYLTKEGKLYFEIGYKQAEGIKDMLQAYFPQRHIRVVTDIFGKERMVVVDYG
                     VSS"
     gene            7146..7736
                     /locus_tag="VC122_001003"
     CDS             7146..7736
                     /locus_tag="VC122_001003"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014635462.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="threonylcarbamoyl-AMP synthase"
                     /protein_id="HEP5640935.1"
                     /translation="MEYLASIIESGDALVLPTETVYGLFAKALDEKAVNAVYDLKQRP
                     RDKAMNLNVADFNSILAFSKEQPRYLKKLYQAFLPGPLTIILKANDQVPYWINSGLST
                     VGFRLPSHPITTALIQKTGPLIGPSANLSGKASGRVFDHIMQDFDFQVFGYADDPFLT
                     GKDSTILDLSGERAVILRQGAITKEELLANVPELRF"
     gene            7754..8206
                     /locus_tag="VC122_001004"
     CDS             7754..8206
                     /locus_tag="VC122_001004"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011528633.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HEP5640936.1"
                     /translation="MLRPLSKTDCPMLQEINAKALGYLVSLDLLERQYERLIEDCHHY
                     FLAYADKDTNQLLGYVHAERYETLYASDGLNLLGLAVLPAYQRRGIGSALLRALESQA
                     RQEGIAFIRLNSASHRKEAHAFYRNLDYADDKTQLRFIKNLQGDDNDF"
     gene            8196..9452
                     /locus_tag="VC122_001005"
     CDS             8196..9452
                     /locus_tag="VC122_001005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003053314.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine hydroxymethyltransferase"
                     /protein_id="HEP5640937.1"
                     /translation="MIFDKGNVEDFDKELWDAIHAEEERQEHHIELIASENMVSKAVM
                     AAQGSVLTNKYAEGYPGNRYYGGTECVDIVETLAIERAKKLFGAAFANVQAHSGSQAN
                     AAAYMALIEAGDTVLGMDLAAGGHLTHGSPVNFSGKTYHFVGYSVDADTEMLNYEAIL
                     EQAKAVQPKLIVAGASAYSRSIDFEKFRAIADHVGAYLMVDMAHIAGLVAAGVHPSPV
                     HYAHIVTSTTHKTLRGPRGGLILTNDEALAKKINSAVFPGLQGGPLEHVIAAKAVAFK
                     EALDPAFKDYAQAIIDNTAAMAAVFAQDDRFRLISGGTDNHVFLVDVTKVIANGKLAQ
                     NLLDEVNITLNKNAIPFETLSPFKTSGIRIGCAAITSRGMGVKESQTIAHLIIKALVN
                     HDQETILEEVRQEVRQLTDAFPLYKK"
     gene            9459..10436
                     /locus_tag="VC122_001006"
     CDS             9459..10436
                     /locus_tag="VC122_001006"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011184511.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoid-associated protein"
                     /protein_id="HEP5640938.1"
                     /translation="MLDSYIKRIVIHQFSPNDTELLLSDRLVSITPRIDEYFRKKLAK
                     VFSDEAKRGQFEANNTFFTTIGDDLLETSVTIAQLWKEAFVISEDQKTNDLVFVQFDK
                     DGEPFFAFLRIALKEQFAHLSDNYEHPFTVTQNNLPSPTQTPDEALVINLKSGQYYLI
                     EKRVKHNGSFANYFSEHLLKVTPEQSVKKSIKMIEQTAQKIAEHFNQDDFTFQSKMKS
                     TLFKQLEADDVLSPEKLADQLFDDNLTARLTFVDQVKDVIPEPIKISDIDHSRQIKKL
                     ENQKLSLSNGIELTVPNAIYQDAEAVEFLLNDDGTYSILIKNIEDIKTK"
     gene            10437..11036
                     /locus_tag="VC122_001007"
     CDS             10437..11036
                     /locus_tag="VC122_001007"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014407575.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysozyme family protein"
                     /protein_id="HEP5640939.1"
                     /translation="MFRLLKRACSFLLLFVIYQSFVIHHNVQRVLAYKPMVEKTLAEN
                     DTKANVDLVLAMIYTETKGGEADVMQSSESSSGQKNSITDSQASIEHGVNLLSHNLAL
                     AEEAGVDSWTAVQAYNFGTAYIDYIAKHGGQNTVDLATTYSKTVVAPSLGNTSGQTYF
                     YYHPLALISGGKLYKNGGNIYYSREVHFNLYLIELMSLF"
     gene            11046..12764
                     /locus_tag="VC122_001008"
     CDS             11046..12764
                     /locus_tag="VC122_001008"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011888875.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEP5640940.1"
                     /translation="MKRLRPYLKGYLKESILGPLFKLLEALFELLVPLLIANMIDISI
                     SQHNSQGILRVVLTLFGLATIGLLLSVTAQYFSSKAAVGFTRQMTDDLFKKIMFLSKE
                     DQDHLGYASLVSRLTSDSFQIQTGINQFLRLFLRAPIIVCGAMVMAYWISPSLTLWFV
                     MMVIVLLTLVFVMSHLLGPLYLLIRRETDHLVRLTSQQLQGIRVIKAFNQTQKELQAF
                     KQQNMLLSRHQYQAATLANVLNPMTFLVVNLTLLILIWQGSWQVAHRSLSQGMLVALI
                     NYLLQILAELLKMTMLMGTINQSVTAAKRINQVFVLADEVPLPLLKDGPISTHLLTIR
                     HLTFTYPGAAEPSLYDIQLSADQGEWIGIIGGTGAGKTTLIDLICQTYSQYSGEISLN
                     WQGEVPKTLTEWRNVIALVPQKAQLFKGTIRSNLLLGQSMPINDEELWRALELAQAKE
                     FVAALPEQLEAPVEAFGRHFSGGQRQRLAIARALLKPKPILILDDASSALDNETRGRL
                     FKALKEELSDVLVILVTQSIKNLQFADKILVLEQGHQLDFASHDQLKVSNALYQEMLA
                     LYVKGE"
     gene            12767..14494
                     /locus_tag="VC122_001009"
     CDS             12767..14494
                     /locus_tag="VC122_001009"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002984648.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEP5640941.1"
                     /translation="MIKTDHHLLKRVLQDLLKKPLPVCILVIASFVQVGLSVYLPVLI
                     GKAVDMSLSVNSWQTLKWLLGQMLVVIVVNTLIQWVMPLVYSRLLYQYSQQLKDKLLE
                     KIHRLPFAYLDRQTIGDLVSRVITDTEQLINGLQLVFNQFILGLLTILCTIIAMAQID
                     WLMLILVLVLIPSSLFLARFIAQKSFHYAQAQTKSRGNLAQFTEEVLRQEGLVQLFNA
                     QEQSICDYHVLNKTYCEASQKAIFYASTVNPATRFINSVIYALLAGLGAVRIMAGLFS
                     VGQLTTFLNVVVQYTKPFNDISSVLAEIQSSLACAQRLYDLLDIEIKEQEHFLTFKAS
                     AVKGQIDFEEVSFSYQKDRPLLKDINFSVPAGSKVAIVGPTGAGKSTLINLLMRFYEL
                     DAGSIKLDKVPIKCYAKEELRSITGMVLQETWLKDATVHELIAYGSEEASRGEVVAAA
                     KAAHAHFFIMQLPKTYDTYLSASDDALSQGQLQLLAIARMFLKKPKVLILDEATSSID
                     IRTEAVIQEALKELMRGRTSFIIAHRLSTIQSADLILVMDQGRLVEWGTHASLMSKNG
                     CYARLQKIE"
     gene            14735..16105
                     /locus_tag="VC122_001010"
     CDS             14735..16105
                     /locus_tag="VC122_001010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012560709.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HEP5640942.1"
                     /translation="MSKIVVVGANHAGTACIKTMLTNYGDANEIVVFDQNSNISFLGC
                     GMALWIGEQIAGPEGLFYSDKEELESLGAKVYMESPVQSIDYDTKTVTALVDGKNHVE
                     TYDKLIFATGSQPILPPIKGAEIKEGSLEFEATLENLQFVKLYQNSADVIAKLENKDI
                     KRVAVVGAGYIGVELAEAFQRKGKEVVLIDVVDTCLAGYYDRDLTDLMAKNMEEHGIQ
                     LAFGETVKEVAGNGKVEKIITDKNEYDVDMVILAVGFRPNTTLGNGKIDLFRNGAFLV
                     NKRQETSIPGVYAIGDCATIYDNATRDTNYIALASNAVRTGIVAAHNACGTDLEGIGV
                     QGSNGISIYGLHMVSTGLTLEKAKRLGFDAAVTEYTDNQKPEFIEHGNFPVTIKIVYD
                     KDSRRILGAQMAAREDMSMGIHMFSLAIQEGVTIEKLALTDIFFLPHFNKPYNYITMA
                     ALGAKD"
     gene            complement(16264..17247)
                     /locus_tag="VC122_001011"
     CDS             complement(16264..17247)
                     /locus_tag="VC122_001011"
                     /EC_number="1.1.1.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003050541.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-lactate dehydrogenase"
                     /protein_id="HEP5640943.1"
                     /translation="MTATKQHKKVILVGDGAVGSSYAFALVTQNIAQELGIIDIFKEK
                     TQGDAEDLSHALAFTSPKKIYAADYSDCHDADLVVLTAGAPQKPGETRLDLVEKNLRI
                     NKEVVTQIVASGFKGIFLVAANPVDVLTYSTWKFSGFPKERVIGSGTSLDSARFRQAL
                     AAKIGVDARSVHAYIMGEHGDSEFAVWSHANVAGVGLYDWLQANRDIDEQGLVDLFIS
                     VRDAAYSIINKKGATFYGIAVALARITKAILDDENAVLPLSVFQEGQYEGVEDCYIGQ
                     PAIVGAYGIVRPVNIPLNDAELQKMQASANQLKAIIDEAFAKEEFASAAKN"
     gene            17438..19924
                     /gene="gyrA"
                     /locus_tag="VC122_001012"
     CDS             17438..19924
                     /gene="gyrA"
                     /locus_tag="VC122_001012"
                     /EC_number="5.6.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002984643.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA gyrase subunit A"
                     /protein_id="HEP5640944.1"
                     /translation="MQDRNLIDVNLTSEMKTSFIDYAMSVIVARALPDVRDGLKPVHR
                     RILYGMNELGVTPDKPHKKSARITGDVMGKYHPHGDSSIYEAMVRMAQWWSYRHMLVD
                     GHGNFGSMDGDGAAAQRYTEARMSKIALELLRDINKNTVNFQDNYDGSEREPVVLPAR
                     FPNLLVNGATGIAVGMATNIPPHNLAESIDAVKMVMEHPDCTTRELMEVIPGPDFPTG
                     ALVMGRSGIHRAYDTGKGSIVLRSRTEIETTQTGRERIVVTEFPYGVNKTKVHEHIVR
                     LAQEKRLEGITAVRDESSREGVRFVIEIRREASATVILNNLFKLTSLQTNFSFNMLAI
                     ENGVPKILSLRQIIDNYISHQKEVIIRRTRFDKDKAEARAHILEGLLIALDHLDEVIA
                     IIRNSETDVIAQTELMSRFDLSERQSQAILDMRLRRLTGLERDKIQSEYDDLLALIAD
                     LSDILAKPERIITIIKEEMDEIKRKYANPRRTELMVGEVLSLEDEDLIEEEDVLITLS
                     NKGYIKRLAQDEFRAQKRGGRGVQGTGVNNDDFVRELVSTSTHDTLLFFTNFGRVYRL
                     KAYEIPEYGRTAKGLPIVNLLKLEDGETIQTIINARKEETAGKSFFFTTKQGIVKRTE
                     VSEFNNIRQNGLRALKLKEGDQLINVLLTSGQDDIIIGTHSGYSVRFNEASIRNMGRS
                     ATGVRGVKLREDDRVVGASRIQDNQEVLVITENGFGKRTSATDYPTKGRGGKGIKTAN
                     ITPKNGQLAGLVTVDGTEDIMVITNKGVIIRTNVANISQTGRATLGVKIMKLDADAKI
                     VTFTLVQPEDSSIAEINTDRENSISKNKDN"
     gene            19944..>20685
                     /locus_tag="VC122_001013"
     CDS             19944..>20685
                     /locus_tag="VC122_001013"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012560711.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sortase"
                     /protein_id="HEP5640945.1"
                     /translation="MVKKQKRRKIKSMSWARKLLIAVLLILGLALLFNKPIRNTLIAR
                     NSNKYQVTKVSKKQIKKNKEAKSTFDFQAVEPVSTESVLQAQMAAQQLPVIGGIAIPE
                     LGINLPIFKGLGNTELIYGAGTMKEEQVMGGENNYSLASHHIFGITGSSQMLFSPLER
                     AQNGMSIYLTDKEKIYEYIIKDVFTVAPERVDVIDDTAGLKEVTLVTCTDIEATERII
                     VKGELKTEYDFDKAPADVLKAFNHSYNQV"
BASE COUNT         6396 a         3648 c         4294 g         6347 t
ORIGIN      
        1 tacctctaat catcagaggt cataggaaga ttattttgta tagaggactt agagaagaag
       61 gtgagaactg acttgttttt cttaatctat gttaaaagag gagaaactta tgtttaatga
      121 catccctgta tttgattatg aagatattca actgattcct aacaagtgca ttattactag
      181 tcgttcacaa gcagatacaa gtgtgacact cggaaaatac cagttcaagc taccagttat
      241 cccagcaaat atgcaaacca ttattgatga aacaattgct gagcggttgg caaaagaggg
      301 ttatttttat attatgcatc gttttgatga agatagccgc aaacctttta tcaaacgcat
      361 gcatgaacaa ggcttgattg catcaatctc tgttggggtt aaagcttgtg aatatgaatt
      421 tgtgacctct ttgaaagagg atgctcctga gtttatcacc attgatattg ctcatggtca
      481 tgctaacagt gttattgata tgattaagca tatcaagact gaattaccag aaacatttgt
      541 cattgctgga aatgttggta cgccagaagc tgtacgagaa ctcgaaaatg caggtgctga
      601 tgctactaaa gtaggcatcg gtccaggcaa agtatgtatc actaaagtta aaaccggttt
      661 cggtacaggt ggttggcagc tagcagcact gcgctggtgc gcaaaggcag ctcgtaaacc
      721 tatcattgct gatggtggga ttcgcacaca tggtgatatt gctaaatcca tccgctttgg
      781 agctagtatg gtcatgattg gatcgctttt tgcaggacat attgaaagtc ctggaaaaac
      841 ggtagaagtg gatggtgaaa cgtttaaaga atattatggc tcggcgtcag aatatcaaaa
      901 aggcgagcat aaaaatgtag aaggcaaaaa aatcttattg cctacaaaag gtcacttatc
      961 agataccctg accgaaatgc aacaggattt gcaatcgtcc atttcttacg cgggtggtaa
     1021 agatttagac agtttgcgtc atgttgatta tgttattgtc aaaaattcca tttggaatgg
     1081 tgattccatt taaatgatat ggagttagct aggaccagta aagaattttt tagcttaatg
     1141 ctggagatgc ttttatcata tctctgtagg tattgaaaaa aataagaaac atggtaaaat
     1201 agtaaattat cataatgaat tcaataatga catacttatt tatgctgtga attggcgcag
     1261 cgtctctaca agacacctta atgtctaaca ataagtaagc ttttaggctt gcttggtttt
     1321 taaccttgca aagtgagaaa ctcttatagt ttctcacttt gcggcaggct ttttctattt
     1381 gaatcaaaag gagatttatg cagttacttg aagaacgcat cttaacagat ggcaatattc
     1441 taggagagaa tattctaaag gtagataatt ttttaactca tcaagttgat taccggttga
     1501 tgaaagcaat tggtaaagtg tttgctcaaa aatatgctga ggctggcatt acaaaagtgg
     1561 ttacaatcga agcttcaggt attgcaccag ccgtatacgc tgcagaagca atggatgttc
     1621 ctatgatttt tgcgaaaaaa cataaaaaca ttaccatgac agaaggcatt ttgacagcag
     1681 aagtttattc tttcactaaa caagtgacaa gcacggtgtc tatcgctggt aaattcctat
     1741 ctaaagaaga caaggttttg attattgatg actttttagc taatggtcag gcagccaaag
     1801 gcttgatcga gattattggt caagcagggg cacaagtcgt cggcgttggt attgtgattg
     1861 agaaatcttt ccaagatggt cgtcgattga ttgaagatat gggcattgaa gtgacctcat
     1921 tagcacgtat taaaaacttt gaaaatggta atctaaactt cttggaggct gacgcataat
     1981 gacacattca acaaagcagg aacattctca ctcgcaatca gctgtccttg gcttacagca
     2041 tgtgctttcg atgtatgccg gatctatttt agtaccaatt atgatcgcag gtgctttagg
     2101 ttattccgct agagagttga cctacctgat ttcgacagat attttcatgt gtggtgtggc
     2161 tactttctta cagttaaagt taaccaagca tacgggagtg ggattaccag ttgttttagg
     2221 atgtgctttt caatcagtgg cacctctatc cattattgga gcgcaacaag gttcaggtgc
     2281 catgtttgga gccctcattg cgtcaggaat ttatgtcatt ttagtggcag gtattttttc
     2341 taagattgct cgtttcttcc caccaattgt tacaggatcg gtcattaccg ttattggctt
     2401 aagtctcgta ggtgttgcta tggggaatat gggagataac gtcaaagagc ctacggcaca
     2461 aagcatgatg ttatcattgt tgacgattgt cattattctt ttagtccaaa aatttaccaa
     2521 aggttttgtg aaatccattt ccattttgat tgggcttgta gtgggaactt tggtttcagc
     2581 catgatggga ttagtagata caactcctgt tgtagaggct tcttggattc atgtgctaac
     2641 cccattttat tttggcatgc caacttttga aatcacttct attgtgatga tgtgtatcat
     2701 cgcaacggtt tctatggttg aatcaacagg tgtctactta gcgctttcag atttgactaa
     2761 tgatcaatta gatgaaaaac gtttgcgcaa tggttatcgc tcagaaggga tcgcagtctt
     2821 tctcggtggg ttatttaata ccttccctta cactggtttt tctcaaaacg ttggacttgt
     2881 ccaaatctca ggtattaaaa cccgtcgtcc gatttactac gcagcaggta tccttgtggt
     2941 cattggccta ctccctaaat ttggagcaat ggcacaaatg attccaagcc cagttcttgg
     3001 tggagctatg cttgttctct ttggtatggt tgctcttcaa ggaatgcaaa tgcttaatcg
     3061 tgttgatttc caaaagaacg agtataattt tattattgct gccgtttcca tctcagcagg
     3121 tttaggcttt aatggcacta atctttttgc cagcttacca gagacagctc aaatgttctt
     3181 aacgaatggt attgtgattg ccactttgac atctgttgtc ttaaatttag ttttaaatgg
     3241 caaagacaaa caagatgaat aattaaaaaa cggctaattg agccgttttt tctgtgatca
     3301 gttttgagca aaccgttaac cccttttaat gatagtatgg ttgaaaatag tgtctcaaac
     3361 catttgacat aaggacatgg tgctttcttg ttactagaag accttcaata ttatcaaagg
     3421 tatttaaaag tgtgataatc attgagctat caagaccaaa taaacgggta gcccatatgt
     3481 cacaataaaa agaagaagga gccatgatcg ttaagctaag catatctgtt tctataggat
     3541 agcctgtctg acgatctaag atatgatgat actgtttccc tttggctgta aactgccttt
     3601 cataaattcc agaagtcaca acagagtgat tgttaacctt gatgacacca agatgttgcc
     3661 cacgtttagc atctggtttt tgaatgccaa ttcgaaaagt cttgtcaggg cttttcggat
     3721 taggtccatg aacgcgtaca tttccaccta aattaataag ggcagagtca ataccatctt
     3781 caatcaggta ggccataatc ttatcagtaa tataaccttt tgctaaggct cctaagtcta
     3841 aagccattcc tacttgttgt aaaaagacag tctgtttttt gtcatctata agaacctgtc
     3901 taggatctgt tagagctaac tgttgtgaga taaggttatg actaggcact ctagcatctt
     3961 caaatccaat tcgccaggct tgaacaagtg gcccaatggc gatattaaga ttacttggtg
     4021 ttgacaagct atgagcttta ccaatttgga ttaggttaaa taaatcagag tgaacgctga
     4081 ggggtttaac accagccgcc tgattaatag ccatcaattc agagtttgaa tcattagctg
     4141 aaaaacgatt tttgtaagta tataataagt caatgactct acttagttgt tggcaagctt
     4201 tgtcagattc gatctgaata tcaataactg ttcccattag ctttaattgt tgcgtaacaa
     4261 acataattag tcctcttttt agcagaatac ccccactaag gagtggggga aggtcattat
     4321 gatttttgtt tcatttcacc ttgaggatag taagttcctt caggcatatc gttgataaaa
     4381 acatggatat tttctttagg tgcttttgca atgcgtgaga ctacttcggt aacctcgcga
     4441 gctaattgat ttttttgttc ttgtgagcgt ccctcaaaca agtcaattgt tacaaatggc
     4501 ataatgttac tcctttatgt tattgggcat cattgtaaca taatgctgtg aaaaagtata
     4561 cactgttttc aatggctggg aaaacttcag cctttgtggt aaaataggta gaatactata
     4621 gaaagtagga ttttccgctt tggctcaact ctattataaa tatgggacaa tgaattctgg
     4681 taaaaccatt gaaattttaa aagttgctca taactatgaa gaacaaggca aacctgttgt
     4741 cattatgaca agtgctcttg atacacgtga tggttttggc atcgtctcaa gccgtattgg
     4801 tatgcgacga gaagctatcc ccatttcaaa tgacatggat attttcacat ttattgccca
     4861 attggaggaa aaaccttatt gtgtcctgat tgacgaaagc caatttttaa gcaagcaaaa
     4921 tgtttatgat ttggcgcgtg tggtggatga gctaaatgtt cctgtcatgg cttttggttt
     4981 gaaaaatgat ttccaaaaca atctttttga aggttcaaaa catttgctat tattggccga
     5041 taagattgat gaaatcaaaa caatttgcca atactgtagt aagaaagcaa ccatggtttt
     5101 aaggacagaa aacggaaaac ctgtctatga aggtgatcag atacaaattg gtggtaatga
     5161 aacatatatc ccagtttgtc gaaaacatta ttttaatcca gagatttaaa aggtaatcgg
     5221 ttttaaagga gatacaaaga aaaatgaata tttatgatca attgcaggca gttgaagatc
     5281 gttatgaaga gttaggtgag ttacttagtg atccagatgt tgtttcagat acaaagcgat
     5341 ttatggaatt atcacgtgaa gaagctaata ctcgagaaac tgtcacagct tatagagaat
     5401 ataagcaagt cattcaaacc atttcagatg ctgaagaaat gatcaaagat gccagtggtg
     5461 atcctgaatt agaagaaatg gctaaagaag aacttaaaga atcaaaagca gctaaagaag
     5521 agtatgaaga aaaattaaaa atccttctat tacctaaaga tcctaacgat gataaaaaca
     5581 ttattttaga aattcgtggt gctgctggtg gcgatgaagc agcgcttttt gctggtgatc
     5641 ttttaacaat gtatcaaaaa tatgctgaaa ctcaaggatg gcgttttgaa gttatggaat
     5701 catccgttaa tggtgttggt ggtattaaag aagtggttgc catggtttct ggtcagtcag
     5761 tttattctaa gcttaaatat gaatcaggag ctcatcgtgt tcaacgtgtc ccagtgacag
     5821 agagtcaggg tcgtgttcat acttcaactg caactgtttt ggtaatgcca gaagttgaag
     5881 aagtagaata cgacattgat cctaaagacc ttcgcgtcga tatttaccac gcgtctggtg
     5941 ctggtggtca gaatgtcaat aaagttgcta ctgcagttcg tatggtacac attcctacag
     6001 gaatcaaagt tgaaatgcaa gaagagcgta ctcagcaaaa aaaccgtgat aaagcgatga
     6061 aaatcattcg tgctcgtgtt gctgaccatt ttgcgcaaat tgctcaagat gaacaagatg
     6121 ctgaacgtaa atcaacagtt ggtactggtg atcgttcaga acgtattcgt acttataatt
     6181 tcccacaaaa tcgtgtaaca gatcatcgta ttggtttgac acttcaaaaa ttagatacta
     6241 ttttatctgg aaaaatggat gaagttattg atgcactcgt tatgtatgat cagacaaaga
     6301 aattagaatc tctcaataac taatgaacta tgctacatta atcagaactt atgaagataa
     6361 actagaacaa atagatgagg atcgcgaaaa tctctcctat gtttttagag aaatcaaaga
     6421 atggtcaagt ctagatatgt tgattcacca aaatcaagct gtgactcctg aagatgctgt
     6481 tcttttggaa catattttct gttctctaag tcagcattta tctcctcaat atattacagg
     6541 taatgcttat tttcgtgatt tgaagcttgc agtcgacaag cgtgtattaa ttccaaggcc
     6601 agaaacagaa gaattagttg acatgatttt agctgaaaat cttgatgcac cgttgaatgt
     6661 tttagatatt ggtacaggta gtggagcgat tgctatttct ctcaaaaaag aaaggcctaa
     6721 ctggcaagta acggcatctg atatttctag agctgctctt gacttggcaa aagccaatgc
     6781 tgatgcttac cagcttgata taacgtttat tgaatcagat gtttttagtc tcatttcagg
     6841 aacatttgat attattgtgt caaatcctcc ctacatatcc tacgaggata aggaagaagt
     6901 cagtctaaat gttttgcaat cagagcctca tttggcactt tttgctaaag aaaatggcta
     6961 tgctatttac cgtaagatta ttgaacaagc tgacaattac ttaactaagg aggggaagct
     7021 ttactttgaa attggttata agcaagctga aggtataaaa gatatgttac aagcctattt
     7081 tcctcaaaga catatacgag tcgtaacgga tatatttggc aaagaaagaa tggtggttgt
     7141 tgattatgga gtatctagct agtatcattg aatcaggtga tgcccttgtt ttgccaacag
     7201 aaacggttta tggtcttttt gccaaggctt tagatgaaaa agcagtaaat gctgtttatg
     7261 acttaaaaca aagaccccga gataaagcaa tgaaccttaa tgtggctgat tttaatagta
     7321 tcttagcctt ttcaaaagaa cagccgagat acctcaagaa attataccaa gcctttttac
     7381 caggtccgtt aacgattatt ttaaaagcta atgaccaggt tccttattgg attaattcgg
     7441 gtctttcaac ggttggtttt cgcttaccca gtcatccaat taccacagct ttaattcaaa
     7501 aaacaggccc tttaattggt ccttctgcaa acttatctgg caaagctagt ggtcgagttt
     7561 ttgatcatat tatgcaagat tttgactttc aagtgtttgg ttatgcagat gatccttttc
     7621 taacaggtaa ggattcaacc atcttagact tatcaggtga gcgtgctgtg attttacgtc
     7681 aaggtgctat caccaaagaa gaattattag ccaacgtgcc tgaacttcgc ttttaagaga
     7741 ggagccgctt ggaatgttga gaccattaag taaaacggac tgtcctatgc tacaagaaat
     7801 taacgccaaa gcccttggct atttagtttc gcttgacttg ttagaaaggc aatatgaacg
     7861 attgattgag gattgccatc actatttttt agcttatgcc gacaaggata ccaatcaatt
     7921 attaggctat gttcacgctg aacggtatga gaccctttat gcttctgatg gtcttaatct
     7981 cttgggcttg gctgttttac cagcctatca gagaagggga attgggagtg ccttactaag
     8041 ggccctggaa tcccaagcta ggcaggaagg cattgccttt attcgcctca attctgccag
     8101 tcatcgtaaa gaagcgcatg ctttttaccg aaacctcgac tatgcagatg ataaaacaca
     8161 attacgattc atcaaaaact tacaaggaga tgacaatgat ttttgataaa ggtaatgttg
     8221 aagactttga taaagagctt tgggatgcca ttcatgctga agaagaaaga caagaacatc
     8281 atatcgaatt gattgcatct gaaaacatgg tttcaaaagc tgtcatggca gctcaaggtt
     8341 ctgttttaac caataagtat gcagaaggct atccaggtaa tcgctattat ggtggaacag
     8401 aatgtgtgga tattgtcgaa acgcttgcta ttgaacgtgc taagaaactt ttcggtgccg
     8461 catttgccaa tgttcaagcc cattccggaa gtcaagccaa tgcagcagct tatatggcct
     8521 tgattgaggc tggtgacacc gtattaggaa tggatttggc tgcaggtggt catctcacgc
     8581 atggctctcc agtaaatttt tctggaaaaa cctaccattt tgtgggttac tcagtagatg
     8641 ctgatactga aatgttaaat tatgaggcga ttttagaaca agcaaaggcg gtccagccaa
     8701 agcttatcgt tgcaggggct tcggcctact ctagaagcat tgattttgaa aagtttcgtg
     8761 ccattgctga tcacgtaggt gcttacttaa tggtggacat ggctcacatc gctggccttg
     8821 tagcagcagg tgtgcacccg agtcctgtcc attatgcgca tattgtgaca tcaacaaccc
     8881 ataaaacctt acgtggtcct cgtggaggct tgatattaac caatgatgaa gctcttgcta
     8941 aaaagattaa ttcggccgtt ttccctggtt tacaaggagg acctctggaa cacgtgattg
     9001 ctgccaaagc ggttgccttc aaagaagcct tggatccagc ctttaaagac tatgcacaag
     9061 ctattattga taatactgcg gctatggcag cagtttttgc acaagatgat cgtttccgtc
     9121 tgatctcagg tggtacagat aatcatgtct ttttagtaga tgtcactaag gttattgcta
     9181 atggtaaact agcgcaaaat cttctggatg aggttaatat tactctaaat aaaaatgcca
     9241 ttccttttga aaccttatct ccttttaaaa catcaggcat tcgtattggt tgtgcagcta
     9301 taaccagtcg tggcatggga gttaaagaaa gccaaactat cgctcacctc atcatcaaag
     9361 ccttggttaa tcatgatcaa gaaactattc ttgaagaggt tcgtcaggaa gtgcgtcagt
     9421 taaccgatgc cttcccactc tataaaaagt aaggaatcat gttagatagt tatataaaac
     9481 gtattgtcat tcatcaattt tctcccaatg acactgagtt actgttatca gatcgtttag
     9541 tgagcattac accacgaatt gatgaatatt tccggaaaaa attggctaaa gtgttctcgg
     9601 atgaagccaa acggggtcag tttgaggcta ataatacctt ttttacaacc attggtgatg
     9661 atttgttgga aacgtctgtt accattgccc agttatggaa ggaagccttt gttatttcag
     9721 aggatcaaaa aaccaatgat ttagtctttg tccagtttga taaggatggc gaaccctttt
     9781 ttgctttctt gcgaattgct ttgaaagaac agtttgctca cctttctgac aattatgaac
     9841 atccatttac ggtgacgcag aataatctgc cgagtcccac ccagacacca gatgaagcac
     9901 tagtcattaa tctcaaatct ggtcagtact acttgattga aaagcgtgtg aagcataatg
     9961 gcagttttgc taattatttt tcagaacacc tcttgaaagt tacacctgag caatctgtta
    10021 aaaagtctat caaaatgata gaacaaaccg ctcaaaaaat tgcagagcat tttaatcaag
    10081 atgattttac ttttcagtca aagatgaaat ccactctttt taagcagtta gaagcagacg
    10141 acgttttatc accagaaaaa ttagctgacc aactctttga tgataattta acagcccgtt
    10201 taacctttgt ggatcaagtc aaagatgtca ttccagaacc tataaaaatt agcgatattg
    10261 atcattctag acagataaaa aaactagaaa atcaaaagct atcgttatca aacgggatag
    10321 aattaacagt accaaatgcc atttatcagg atgctgaagc ggttgaattt ttgttaaatg
    10381 atgatgggac ttattcaatc ttgattaaaa atatagagga cattaaaacc aaatagatgt
    10441 ttagactttt aaagcgtgcc tgcagttttc ttcttctttt tgtcatttat caatcctttg
    10501 ttattcatca caatgttcaa cgtgttttgg catataagcc tatggttgag aaaacattag
    10561 cagaaaatga tacaaaggca aatgtagact tagtcttagc tatgatatac accgagacaa
    10621 aaggaggaga agcagatgtc atgcaatcca gtgaaagcag ttcaggacaa aaaaactcga
    10681 ttacagatag tcaggccagt attgagcacg gggtgaattt actgtctcat aatcttgctc
    10741 tagctgagga agcaggagta gattcttgga cggctgttca ggcctataat tttggaacag
    10801 cttatattga ttatattgct aaacatggag gtcaaaatac cgttgattta gcgactacct
    10861 attccaaaac cgttgtggcg ccaagcctag gcaacactag tggtcaaacc tatttttatt
    10921 accaccccct cgccttgatt tctggaggta agctttataa gaatggcggc aatatctatt
    10981 actcacgaga agttcatttc aatctttatt taattgaact gatgagcctt ttttagaaag
    11041 ttatcatgaa acgattacgt ccatatttga aagggtacct aaaagaaagt atcttaggtc
    11101 ctctttttaa attattagaa gctttatttg aattattagt ccctttgtta attgctaata
    11161 tgattgatat atcgattagt caacacaaca gccagggaat tttgagggtt gttttaacat
    11221 tatttggttt agcaaccatt ggcttattgc tttccgttac agcccagtat ttttcttcga
    11281 aagcagctgt tggttttaca agacaaatga cagatgattt gtttaaaaaa atcatgtttt
    11341 tgagcaagga ggaccaagac catcttggtt atgctagtct ggtatcacga ttgaccagtg
    11401 atagttttca aattcaaact ggtatcaatc aatttttgcg tctttttcta agggccccta
    11461 ttatcgtatg tggtgctatg gtaatggctt attggattag tccaagtcta accctatggt
    11521 ttgtgatgat ggtgattgtc ttactaacac ttgtttttgt tatgtcacat ttattaggtc
    11581 ccctttatct cttaatccgc cgagaaacag atcacttggt tcgtttgact agccaacagt
    11641 tacaaggaat ccgagtaatt aaggctttta atcaaaccca aaaagaactc caagcattta
    11701 agcagcaaaa tatgctctta agtcgccacc aatatcaagc tgctacccta gccaatgtct
    11761 tgaatcctat gactttttta gtggtcaatc ttactctgtt aatcttgatt tggcaaggaa
    11821 gttggcaagt agctcataga tcgcttagtc agggcatgtt ggttgcttta attaattacc
    11881 tcttgcagat tttggcagaa cttttgaaaa tgaccatgtt gatgggaacc atcaatcaaa
    11941 gtgtaacagc cgcaaaacgg attaatcagg tgtttgtttt agctgatgaa gtgcctttgc
    12001 ccttacttaa agatggtcct atttcaactc atctgttgac catccggcac ttgaccttta
    12061 cttatccagg agcagcagag ccaagtctgt atgatattca gttaagtgct gatcagggag
    12121 aatggattgg catcattggt ggtactggag caggcaaaac gactttgatt gacttgatct
    12181 gccaaactta ttcacaatat agtggtgaga ttagtcttaa ttggcaagga gaagtgccaa
    12241 aaacactaac cgagtggcga aatgtgattg ctcttgttcc tcaaaaagct caattattta
    12301 aaggaacgat tagaagtaac ctcctactgg gacaatcaat gcctattaat gatgaggagc
    12361 tgtggcgggc tcttgaatta gctcaagcaa aagagtttgt cgctgcctta ccagaacaat
    12421 tagaggcacc tgttgaagcc tttggccgtc atttttcagg agggcaacgg caacgtctag
    12481 ctattgccag agcacttctg aagccaaaac ccattttgat tttggatgat gccagttccg
    12541 ccttagacaa tgagactcga ggacgacttt ttaaagctct aaaagaagag ttatcagatg
    12601 ttttagtgat tctagtcacg caatccatta agaatttgca gtttgctgat aagattttag
    12661 tgttggaaca gggccatcaa ttggattttg ccagtcatga ccagttaaaa gtaagcaatg
    12721 cactttatca agaaatgcta gcgctttacg tgaaggggga gtaaaaatga ttaaaacaga
    12781 ccatcacctc ctaaagcgag tgcttcagga tttgttaaaa aaacctctac ctgtttgtat
    12841 tctcgtgata gctagttttg tacaagttgg tttaagtgtt tacttgcctg ttcttatcgg
    12901 taaagccgtt gatatgagtt tgtcagtcaa ttcttggcaa actttaaaat ggctgttggg
    12961 gcaaatgtta gttgttattg tggtcaacac attgattcaa tgggtgatgc ctcttgtcta
    13021 tagtcgtttg ctatatcaat atagccagca attgaaagat aagcttttgg aaaaaattca
    13081 tcgtcttcct tttgcatatc tagatcgtca aaccattgga gatttggtta gtcgagtgat
    13141 aaccgacact gagcaactga tcaatggtct acaattggtt tttaaccagt ttatacttgg
    13201 actattgacg attctgtgta ccattatagc tatggcacag attgactggc tcatgttaat
    13261 cttggttttg gtgttaatac caagctccct ctttttagct cgttttattg ctcaaaagag
    13321 ttttcattat gcacaagcac agaccaaaag cagaggaaat ttagctcagt ttactgaaga
    13381 agtccttcgt caagaaggtt tggttcaact gtttaatgct caagagcagt ctatctgtga
    13441 ttatcatgtt ttaaataaaa cctattgcga ggcttcacaa aaagccattt tttacgcctc
    13501 aacagtcaat ccagccactc gctttattaa tagtgtcatt tatgctttac tggcaggttt
    13561 aggggctgtt cgtatcatgg ctggactttt ttcagtaggt cagctaacca cttttttaaa
    13621 tgttgttgtc caatatacca aacctttcaa cgatatttca tctgtcttgg cagagataca
    13681 gagttcttta gcttgtgcgc aaaggcttta tgacctttta gatattgaaa taaaagagca
    13741 agagcacttt ctaacattta aggcatcagc ggttaaggga caaattgact ttgaagaagt
    13801 aagtttttct tatcaaaaag acaggccctt actgaaggat attaattttt ctgttcctgc
    13861 gggttccaag gtagcgattg tagggcctac aggtgcaggc aaatcgactt taatcaattt
    13921 actcatgcgc ttttatgaac ttgatgctgg tagcatcaag ttagataaag ttcctattaa
    13981 gtgttatgct aaggaagaac ttaggtccat tactggcatg gtattgcaag aaacctggtt
    14041 gaaagatgcg actgttcatg agttgattgc ttacggcagt gaagaggcta gccgtggtga
    14101 agtagtggca gcagccaaag cagctcatgc acacttcttt attatgcaac ttcctaagac
    14161 ttatgatact tacttaagtg cttctgatga tgctttgtcc caagggcagc tccagttatt
    14221 agctattgcc agaatgtttt tgaaaaaacc aaaagtcttg attctagatg aagccacctc
    14281 ctctattgat attagaacag aagctgttat tcaagaggca ctaaaagaac tcatgagagg
    14341 aaggaccagc tttatcattg cccatcgttt atcaacgatt caatcagctg atttgattct
    14401 tgttatggat caaggtcgat tggttgagtg gggaacacat gccagcttaa tgtcaaaaaa
    14461 cggatgttat gctagattac aaaagataga ataaagttcg tcttttaaca aaggtgaact
    14521 ttttaagtct tcctaaagtt agttaattaa caaactttaa actgttatat aaaaaaataa
    14581 cagcgaaaaa gcggacgatc tttttttata agtgagaaaa aacagttgac taaagaagaa
    14641 ataactcaaa aaagtgagca atatatttgg aagcgttttt tgactgtggt ataatagagc
    14701 cctatataat attgtgaaat gaggaacaaa atatatgagt aaaatcgttg ttgttggtgc
    14761 aaaccatgct ggtacggcct gtatcaagac tatgttaaca aactacggtg atgctaatga
    14821 gattgttgta tttgaccaaa actcaaatat ttcattttta ggctgtggta tggcactttg
    14881 gattggtgag caaattgctg gaccagaagg acttttctat tcagataaag aagaattaga
    14941 gtctttaggg gctaaagttt acatggaatc acctgttcaa tcaatcgact acgataccaa
    15001 aacagtgaca gcgcttgttg atggtaaaaa ccacgtggaa acttacgaca agttgatttt
    15061 tgcaactggc tcacaaccta tcttaccacc gattaaaggt gccgaaatca aagaaggatc
    15121 acttgaattt gaagcaactc ttgaaaatct tcaatttgtg aagttatacc aaaactcagc
    15181 tgatgtgatt gcaaagcttg aaaacaaaga cattaaacgt gtagccgtag ttggtgctgg
    15241 ttacattggt gttgagttag ctgaagcttt ccaacgcaaa ggcaaagaag tggttctgat
    15301 tgatgtcgtg gacacttgct tggcaggtta ttacgaccgt gatttgactg acttaatggc
    15361 taaaaacatg gaagaacatg gtattcaact agcctttggt gaaacagtta aagaagtagc
    15421 tggtaatggt aaggttgaaa agatcattac tgacaaaaat gagtacgatg tggatatggt
    15481 tatcctcgcc gttggtttcc gtccaaacac aactcttgga aatggtaaga ttgatctttt
    15541 ccgtaatggt gctttccttg tcaataaacg ccaagaaact tctattccag gtgtttatgc
    15601 tattggtgac tgtgcaacta tctacgataa tgctactcgc gatacaaact acattgcttt
    15661 agcttcaaat gctgtccgta caggaattgt agcagcacat aacgcttgtg gtacagacct
    15721 tgaaggtatt ggcgttcaag gttcaaacgg tatttccatt tacggcttgc acatggtatc
    15781 aactggtttg actcttgaaa aagctaaacg acttggtttt gatgctgcag tgactgagta
    15841 tactgataat caaaaacctg aatttatcga acacggtaac ttcccagtaa ccattaagat
    15901 tgtttacgat aaagactcac gtcgtatctt gggtgctcaa atggcagccc gtgaagatat
    15961 gtcaatggga attcacatgt tttcacttgc tatccaagaa ggagtgacca ttgaaaagtt
    16021 ggcattaact gatattttct tcttaccaca tttcaacaaa ccttacaact acatcacaat
    16081 ggcagcactt ggtgccaaag actagtatct gataggaaaa agccagagca gttcagcagc
    16141 actggctttt ttgcttttta attgttgcaa cctgaaaagt tctaaactta tgattatcct
    16201 cttatagcac gttggaaaag gaatatcagg caaaacaaaa agagaccgaa gtctcttttg
    16261 tgattagttt ttagcagcag aagcaaattc ttccttagca aaggcttcat caatgatagc
    16321 tttcaattga tttgcagagg cttgcatttt ttgcaattca gcatcgttaa gtggaatgtt
    16381 tactggacga acaattccat acgctccaac gatagctggt tgaccaatat agcaatcttc
    16441 aacaccttcg tattggcctt cttggaaaac agaaagcgga agaacagcat tttcatcatc
    16501 aagaatagct tttgtaatac gagctagggc aactgcaata ccgtagaaag tagcaccttt
    16561 tttattgatg attgagtaag cagcatcacg tacagaaatg aaaagatcaa ctaggccttg
    16621 ctcatcaatg tcacgattag cttggagcca gtcgtaaaga cctacaccag caacgttagc
    16681 gtgtgaccaa acagcaaatt cagagtcacc gtgttctccc atgatgtaag cgtgaaccga
    16741 acgcgcatca acaccgattt tagcagccag agcttgacgg aaacgggctg aatctagaga
    16801 tgtacctgaa ccaatgacac gctctttagg gaaacctgag aatttccatg ttgagtaagt
    16861 caaaacgtca actgggttag cagcaacaag aaagattcct ttgaaaccag atgcaacgat
    16921 ttgtgtaaca acctctttat tgatacgaag gtttttttca accaggtcaa ggcgtgtttc
    16981 acctggtttt tgaggagcac cagctgttaa gacaacaaga tcagcatcat gacagtctga
    17041 gtagtcagct gcatagattt ttttaggtga tgtaaaagct agagcgtggc ttaaatcttc
    17101 tgcatctcct tgagtttttt ctttaaaaat atcaataata ccaagttctt gagcaatatt
    17161 ttgtgttacc aatgcaaaag cgtatgatga accgacggca ccatcaccga ccaagattac
    17221 ttttttgtgt tgtttagttg cagtcatttc taaacatctc cttaatttta ttagagcaaa
    17281 agctcatacg gtcttatttt aacatattga taattgtttg tcattgaatt cagaaaaata
    17341 atgaaaatag tttactgata acgcttccaa taagaaaaaa taaggaattt atggtataat
    17401 gaaatctagt attgactaga aaaggagcat tccttaaatg caagatcgaa atttaattga
    17461 cgtcaatctg acaagtgaaa tgaaaacgag tttcattgac tatgccatga gtgtcattgt
    17521 ggcaagagct ttgccagatg tgcgtgatgg tctgaaacct gtgcatcgtc gtattctcta
    17581 tgggatgaat gaactcggtg tgacacctga taaaccgcat aaaaaatctg ctcgtattac
    17641 gggtgatgtc atgggtaaat atcacccgca tggggattct tctatttatg aagctatggt
    17701 tcgtatggct cagtggtgga gttatcgcca tatgcttgtt gatggccatg gcaattttgg
    17761 ttctatggat ggtgatggtg ctgccgctca acgttatact gaagcgcgca tgagtaaaat
    17821 tgcgcttgaa ttattaaggg atatcaataa aaataccgtt aattttcaag ataactacga
    17881 tggaagcgaa agagaaccag tagtattacc agctcgcttt cctaatttat tagtcaatgg
    17941 agcgacaggg attgccgttg ggatggcaac taacattccg cctcataatt tggcagagtc
    18001 tattgatgct gtcaagatgg taatggaaca tccagactgt acaacaagag agttgatgga
    18061 agtgatccct ggacctgact ttccgactgg tgcccttgtt atggggcgct caggtattca
    18121 tagggcttat gatactggta aaggttctat cgttttacgc tctcgaacag aaattgaaac
    18181 aactcaaaca ggtcgggaac gtattgtggt tactgaattt ccctatggcg tcaataaaac
    18241 gaaagtgcat gaacacattg ttcgtttagc acaagaaaaa agactagaag gtattacagc
    18301 agttcgtgac gaatctagcc gtgaaggagt gcgttttgtt atcgagattc gtcgagaggc
    18361 atccgcgaca gttatcttaa ataacctctt taaattaaca agccttcaga cgaatttcag
    18421 ttttaatatg ttggctattg aaaatggtgt gcctaaaata ttgtcactca gacaaatcat
    18481 tgataattac attagtcatc aaaaagaagt tattatacgt cgcacccgat ttgataaaga
    18541 taaagctgaa gccagagctc atattttaga aggcttactg attgcccttg atcatttaga
    18601 cgaagtgatt gctattattc gtaacagtga gacagatgtc attgctcaga cagaattgat
    18661 gtcacgtttc gacttgtctg aacgccaaag ccaagctatt ttggatatgc gtttgcgtcg
    18721 tttgactggt ttggaacgtg ataagattca atcagaatat gatgatttgt tggccttgat
    18781 tgctgactta tctgatattt tagccaaacc agaacgtatt atcactatca tcaaagaaga
    18841 aatggatgag atcaagcgta aatacgctaa cccacgccgt acagaattaa tggtcggtga
    18901 agtcctttct ctagaagatg aagacctaat tgaagaagaa gatgttttga ttaccttgtc
    18961 aaataaaggt tatatcaagc gtcttgctca agatgaattt cgtgcccaga agcgtggtgg
    19021 acgtggcgtc caaggaactg gagtcaacaa tgacgacttt gtccgtgaat tggtatcaac
    19081 aagtacccat gatactttgc tcttctttac gaattttggg cgtgtctatc ggttgaaagc
    19141 ttacgaaatt ccagaatatg gtcgtacagc aaaaggtctt ccaattgtta atctattgaa
    19201 attagaagat ggtgaaacca ttcaaactat tatcaacgca cgtaaggaag aaacagcagg
    19261 taaatccttc ttctttacaa cgaaacaagg cattgtcaaa cgtacagaag tttcagaatt
    19321 taataatatc cgtcaaaacg gtctccgtgc tcttaagttg aaagagggag atcagctgat
    19381 taacgtcctt ttgacaagcg gtcaggatga tattatcatt ggaactcact ccggatattc
    19441 tgttcgtttt aatgaagcaa gcatccgcaa catgggccga tcagctacgg gtgttcgcgg
    19501 ggttaagctt cgtgaagatg atcgcgttgt tggcgcgtca cgtattcaag ataaccaaga
    19561 agttcttgta attaccgaaa atggctttgg taagagaaca agtgctacag actatcctac
    19621 aaaaggtagg gggggaaaag gaataaaaac agctaatatt acccctaaaa atggtcaatt
    19681 agctggattg gttaccgtag atggtacaga agatatcatg gtgattacaa ataaaggtgt
    19741 cattattcgc acaaatgttg caaatatttc gcaaacaggt cgtgctactt tgggtgttaa
    19801 aattatgaaa ctagatgctg atgctaagat tgtaacattt accttggttc agcctgaaga
    19861 tagtagtatc gctgaaatca atactgatcg tgaaaatagt atttcaaaaa ataaagataa
    19921 ttaatcataa caaggagtaa aaaatggtaa aaaaacaaaa gcgtcgaaaa ataaaatcca
    19981 tgtcttgggc aagaaaacta ctaatagctg tacttttaat tctaggttta gcattgcttt
    20041 ttaacaaacc tatccgaaat acattaattg ctcgaaactc taataaatat caagtaacga
    20101 aagtttcgaa aaaacaaatc aaaaagaata aagaggctaa gtcaacattt gatttccaag
    20161 ccgtagagcc agttagtaca gagagtgtct tgcaagcaca aatggcggct cagcaacttc
    20221 ctgttatagg gggcattgcc ataccagagc ttggcattaa tttaccaatt tttaaaggtt
    20281 taggaaatac tgagcttatt tatggcgcag gaacgatgaa agaagaacaa gttatgggag
    20341 gagaaaataa ttattctctt gccagtcatc atatttttgg aattacaggt tcatctcaaa
    20401 tgctcttttc gccgcttgaa agagcacaaa atgggatgtc catctattta acagataaag
    20461 aaaaaattta cgaatacatc ataaaagatg ttttcacggt agctcctgaa cgcgttgatg
    20521 ttatcgatga tacagctggt ctcaaagaag tgactttagt gacttgtaca gatatcgaag
    20581 caacagaacg tattattgtc aaaggagaac taaaaacaga atacgatttt gataaagcgc
    20641 ccgccgatgt attgaaagct tttaatcatt cttataacca agtat
//
LOCUS       DATXIG010000026        20567 bp    DNA     linear   BCT 28-DEC-2023
DEFINITION  TPA_asm: Streptococcus pyogenes strain MGAS11113
            SAMN08555799-rid19682583.denovo.026, whole genome shotgun sequence.
ACCESSION   DATXIG010000026 DATXIG010000000
VERSION     DATXIG010000026.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN08555799
            Sequence Read Archive: SRR6740495
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pyogenes
  ORGANISM  Streptococcus pyogenes
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 20567)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 20567)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-OCT-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-OCT-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001918058.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001918058.2
            Genome Coverage       :: 368x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/12/2023 11:23:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 1,806
            CDSs (total)                      :: 1,769
            Genes (coding)                    :: 1,722
            CDSs (with protein)               :: 1,722
            Genes (RNA)                       :: 37
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1, 1 (5S, 23S)
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 47
            CDSs (without protein)            :: 47
            Pseudo Genes (ambiguous residues) :: 0 of 47
            Pseudo Genes (frameshifted)       :: 30 of 47
            Pseudo Genes (incomplete)         :: 20 of 47
            Pseudo Genes (internal stop)      :: 9 of 47
            Pseudo Genes (multiple problems)  :: 11 of 47
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..20567
                     /organism="Streptococcus pyogenes"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN08555799-rid19682583.denovo.026"
                     /strain="MGAS11113"
                     /serotype="Emm28"
                     /isolation_source="normally sterile site"
                     /host="Homo sapiens"
                     /db_xref="taxon:1314"
                     /country="Finland"
                     /collection_date="2002"
                     /collected_by="National Public Health Laboratory"
     gene            552..941
                     /locus_tag="VC122_001014"
     CDS             552..941
                     /locus_tag="VC122_001014"
                     /EC_number="3.5.4.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010922351.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytidine deaminase"
                     /protein_id="HEP5640946.1"
                     /translation="MGTTDLVSCAVQASEYAYVPYSHFPVGAALKTKDGTIYTGCNIE
                     NVSFGLTNCGERTAIFKAISDGHKELVEIAIYGETMQPVSPCGACRQVMAEFFAPSSL
                     VTLIAKNGQTVEMTVGDLLPYSFTDLI"
     gene            1035..2087
                     /locus_tag="VC122_001015"
     CDS             1035..2087
                     /locus_tag="VC122_001015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003058857.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BMP family protein"
                     /protein_id="HEP5640947.1"
                     /translation="MNKKFIGLGLASVAVLSLAACGNRGASKGGASGKTDLKVAMVTD
                     TGGVDDKSFNQSAWEGLQSWGKEMGLQKGTGFDYFQSTSESEYATNLDTAVSGGYQLI
                     YGIGFALKDAIAKAAGDNEGVKFVIIDDIIEGKDNVASVTFADHEAAYLAGIAAAKTT
                     KTKTVGFVGGMEGTVITRFEKGFEAGVKSVDDTIQVKVDYAGSFGDAAKGKTIAAAQY
                     AAGADVIYQAAGGTGAGVFNEAKAINEKRSEADKVWVIGVDRDQKDEGKYTSKDGKEA
                     NFVLASSIKEVGKAVQLINKQVADKKFPGGKTTVYGLKDGGVEIATTNVSKEAVKAIK
                     EAKAKIKSGDIKVPEK"
     gene            2226..3758
                     /locus_tag="VC122_001016"
     CDS             2226..3758
                     /locus_tag="VC122_001016"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010922350.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEP5640948.1"
                     /translation="MTQHVIEMREITKKFGDFVANDHINLNVRKGEIHALLGENGAGK
                     STLMNMLAGLLEPTSGEIVINDKPVQIDSPSKSAKLGIGMVHQHFMLVEAFTVAENII
                     LGNEVVKNGCLDLNQASKDIKVLSEKYGLAINPSAKVSDISVGAQQRVEILKTLYRGA
                     DILIFDEPTAVLTPAEIKELMTIMKNLVKEGKSIILITHKLDEIRAVAERVTVIRRGK
                     SIETVDVAGATSQDLAEMMVGRSVSFTTSKKAAEPKDVVLSIKNLEVDENRGIPAVKG
                     LSLDVRAGEIVGIAGIDGNGQSELIQAITGLRKVKSGSIMIKNKEVTHLSSRKITELS
                     VGHVPEDRHRDGLILDLSLAENTALQTYYKQPLSQNGILNYTKINDYARQLMKEFDVR
                     GANELVPARGFSGGNQQKAIIAREVDRDPDLLIVSQPTRGLDVGAIEYIHKRLIKERD
                     KGKAVLVVSFELDEILNLSDRIAVVHDGKIQGIVLPENTNKQELGILMAGGSIHKEEG
                     HV"
     gene            3751..4815
                     /locus_tag="VC122_001017"
     CDS             3751..4815
                     /locus_tag="VC122_001017"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017768037.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEP5640949.1"
                     /translation="MSKNAQKIAVPLISVLLGFLLGAIIMVIFGYDPIWGYEGLFQIA
                     FGSVKNIGEIFRSMGPLILIALGFTVASRAGFFNVGLSGQALAGWISAGWFALLNPDM
                     PRPLLILMTALIGMIAGGIAGAIPGILRAYLGTSEVIVTIMMNYIILYVGNAIVQRGY
                     PESVKQSIDSTIQVSDNASYQTHWLSALTNNSRINIGIFFAIIAIALIWFLLNKTTLG
                     FEIRSVGLNPHASEYAGMSSKRTIILSMIISGALAGLGGVVEGLGTFENVFVQGSSLA
                     VGFDGMAVSLLAANSPLGIFFSSFLFGVLNIGAPGMNIAGIPPELVKVVTASIIFFVG
                     SHYLIERYIIRPKKLVKGGK"
     gene            4817..5773
                     /locus_tag="VC122_001018"
     CDS             4817..5773
                     /locus_tag="VC122_001018"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000060306.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEP5640950.1"
                     /translation="MSLVTIFALLVSSMLIYATPLIFTSIGGTFSERSGVVNVGLEGI
                     MVMGAFSGIVFNLEFAETFGKATPWIAVLVGGIVGLIFSLIHAVATINFRADHIVSGT
                     VLNLLAPSFAVFLVKAMYGKGQTDNIQQSFGKFDFPGLSQIPVIGDIFFKNTSLIGYF
                     AIAFSFFAWFLLYKTRFGLRLRSVGEHPQAADTLGINVYLMKYYGVMISGFLGGIGGA
                     VYAQSISVNFAVTTILGPGFIALAAMIFGKWNPVGAMLSSLFFGLSQSLAVIGAQLPL
                     LEKIPTVYLQIAPYMVTIIILAAFFGQAVAPKADGINYIKSK"
     gene            complement(5991..7709)
                     /locus_tag="VC122_001019"
     CDS             complement(5991..7709)
                     /locus_tag="VC122_001019"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002989707.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phospho-sugar mutase"
                     /protein_id="HEP5640951.1"
                     /translation="MTYTENFQKWLDFEQLPDYLRQELLSMDEKTKEDAFYTNLEFGT
                     AGMRGYIGAGTNRINIYVVRQATEGLAKLIETKGEEAKKHGVAIAYDSRHFSPEFAFE
                     SAQVLAQHGIKSYVFEALRPTPELSFAVRHLNAYAGIMVTASHNPAPFNGYKVYGQDG
                     GQLPPADADALTDFIRAIENPFAVELADLDESKSSGLIQVIGEDVDMEYLREVKDVNI
                     NQDLINNFGKDMKIVYTPLHGTGEMLTRRALAQAGFESVVVVESQAKADPDFSTVKSP
                     NPESQAAFALAEELGREVDADVLVATDPDADRLGVEIRQPDGSYKNLSGNQIGAIIAK
                     YILEAHKTAGTLPENAALAKSIVSTELVTKIAESYGATMFNVLTGFKFIAEKIQEFEE
                     KHNHTYMFGFEESFGYLIKPFVRDKDAIQAVLLVAEIAAYYRSRGLTLADGIDEIYKE
                     YGYFAEKTISVTLSGVDGAAEIKKIMDKFRENGPKQFNNTDIVLLEDFQKQTATKNDG
                     TISNLTTPPSNVLKYTLADDSWIAVRPSGTEPKIKFYIATIGDTLDIAQEKIANIETE
                     INTFVG"
     gene            complement(7885..8454)
                     /locus_tag="VC122_001020"
     CDS             complement(7885..8454)
                     /locus_tag="VC122_001020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011054524.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ECF transporter S component"
                     /protein_id="HEP5640952.1"
                     /translation="MNHRKSADISRIAIFFAIMLVIHFVSSLVFNIWPIPIKPTLVHI
                     PVIIASVLYGPRIGAILGGLMGIISVITNTIILLPTNYLFSPFVDHGTFASLIIAIIP
                     RILIGITPYYCYKLIPNQFGLIVSGIIGSLTNTIFVLSGIFIFFATVFDGNIKALLTA
                     IISSNAIVEMIISAIITFVLIPTLSRLKR"
     gene            complement(8512..9057)
                     /gene="coaC"
                     /locus_tag="VC122_001021"
     CDS             complement(8512..9057)
                     /gene="coaC"
                     /locus_tag="VC122_001021"
                     /EC_number="4.1.1.36"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002984544.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphopantothenoylcysteine decarboxylase"
                     /protein_id="HEP5640953.1"
                     /translation="MTKHITLAVSGSISAYKAADLTSQLTKIGYDVHIIMTQAATQFI
                     TPLTLQVLSKNAIHLDVMDEHDPKVINHIELAKRTDLFIVAPASANTIAHLAYGFADN
                     IVTSVALALPATTPKLIAPAMNTKMYQNPITQENIKRLSTIGFTEIPPKSSLLACGDK
                     GPGALADIDVILATIDTIWKL"
     gene            complement(9050..9742)
                     /gene="coaB"
                     /locus_tag="VC122_001022"
     CDS             complement(9050..9742)
                     /gene="coaB"
                     /locus_tag="VC122_001022"
                     /EC_number="6.3.2.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011528675.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphopantothenate--cysteine ligase"
                     /protein_id="HEP5640954.1"
                     /translation="MTMKLIITSGGTTEPIDAVRGITNHSTGQLGKLITERFLQYHHD
                     VTLVTTKTATKPLPNKLLRIIEVETVNDLMAALKDQVPHHDILIHSMAVSDYTPVYMT
                     DLEQVSQADNLNCFLCEHNSEPKISSASDYQVLFLKKTPKVISYVKQWNPNIKLVGFK
                     LLVNVPQEELIKVARASLAKNHADYILANDLVDIQTGMHKALLISNNEVASADTKEAI
                     ADLLYERMTKHD"
     gene            9923..10774
                     /locus_tag="VC122_001023"
     CDS             9923..10774
                     /locus_tag="VC122_001023"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011528674.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoate--protein ligase family protein"
                     /protein_id="HEP5640955.1"
                     /translation="MKGRIVITIRDLASLPIFVYGDGNKKVPGALSPFVWTEVFLKTI
                     NQEPNQLILHIWPMTRTVILGMLDRQLPYFELAKTEIGNNGYVPVTRNIGGLAVVADD
                     GILNFSLVIPDHFSESISISNAYLIMVDVIRESFSDYYQRIEYHEIKNSYCPGNFDLS
                     IAGRKFAGIAQRRIKKGIVVSIYLSVCGDQAARGQLIKDFYEAGTQGEVTKVNYPQID
                     PECMATLSELLETPFTVAEVLERLRLTLRQLGFSLTEKSPDQALLTNFDAVYERMQLE
                     VVRKEGK"
     gene            10771..11970
                     /locus_tag="VC122_001024"
     CDS             10771..11970
                     /locus_tag="VC122_001024"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011528673.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-dependent flavin oxidoreductase"
                     /protein_id="HEP5640956.1"
                     /translation="MKERFSPLFEPLTLPNGSQLDNRFVLSPMVTNSSTKDGYVTQDD
                     VSYALRRAASAPLQITGAAYVDPYGQLFEYGFSVTKDADISGLKELAQAMKAKGAKAV
                     LQLTHAGRFASHALTKYGFVYGPSYMQLRSPQPHEVKPLTGQQIEELIAAYAQATRRA
                     IQAGFDGVEVSSAQRLLIQTFFSTFSNKRTDSYGCQTLFNRSKLTLAVLQAVQQVINQ
                     EAPDGFIFGFRATPEETRGNDIGYSIDEFLQLMDWVLNIAKLDYLAIASWGRHVFRNT
                     VRSPGPYYGRRVNQVVRDYLRNKLPVMATGGMNTPDKAIEALAHADFIGVSTPFVVDP
                     EFAHKIKEGCEESIHLRIRPADLKSLAIPQASFKDIVPLMDYGESLPKESRTLFRSLT
                     HNYKEIK"
     gene            11967..12965
                     /locus_tag="VC122_001025"
     CDS             11967..12965
                     /locus_tag="VC122_001025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014635481.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LLM class flavin-dependent oxidoreductase"
                     /protein_id="HEP5640957.1"
                     /translation="MKVSILDYGVIDKEKTPQEALLETRCLAQVADKLGFHRFWVAEH
                     HNIYAFAISSPELLMMHLADHTKQIRIGSGGIMPLHYSSFKIAEWIMTLEALHPNRID
                     LGIGNSLGTTLVQRALSSIHCKDSYSQVVTELYQYLNPDHLSPLPIFVNPRGNTYPQI
                     WTLSNSLETAELAGQLGLGYTFGIFPYIPKDPITEAKRVSAHYRKAFRPSKLLKIPKL
                     ILAVFIVLSDTDEEAEALAKPLDIWMLGQQDFNEFKTYPDVEEARNYHLTEKQREAIA
                     ANRSRMVIGSPHTVKKQLDRLIEACQADELLAIPLVPEFANRQRTLELLADLYVTI"
     gene            13007..13339
                     /locus_tag="VC122_001026"
     CDS             13007..13339
                     /locus_tag="VC122_001026"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000717324.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine cleavage system protein H"
                     /protein_id="HEP5640958.1"
                     /translation="MKKIANYLLIEKTDDRYTISMTPELQDDIGTIGYAEFTDNDHLA
                     VDDIILNLEASKTVMSVLSPLAGAVVERNEAATLTPTLLNSEKAEENWIVVLTDVDQA
                     AFDALEDA"
     gene            13332..14144
                     /locus_tag="VC122_001027"
     CDS             13332..14144
                     /locus_tag="VC122_001027"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002989718.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein-ADP-ribose hydrolase"
                     /protein_id="HEP5640959.1"
                     /translation="MPSSFDLLGEMIDLLQTEQLTSSWACPLPNALTKRQDLWRALIN
                     QRPALPLSKDYLNLEDAYLDDWRASFVPVSVKDCQKTNYTSLFLYHGDIRYLAVDAIV
                     NAANSELLGCFIPNHGCIDNAIHTFAGSRLRLACQAIMTEQGRKEAIGQAKLTSAYHL
                     PASYIIHTVGPRITKGRHVSPIRADLLARCYRSSLDLAVKAGLTSLAFCSISTGEFGF
                     PKKEAAQIAIKTVLKWQAEHPESKTLTIIFNTFTSEDKALYDTYLQKENNCE"
     gene            14137..15018
                     /locus_tag="VC122_001028"
     CDS             14137..15018
                     /locus_tag="VC122_001028"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002984557.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent deacetylase"
                     /protein_id="HEP5640960.1"
                     /translation="MSNWTTYPQKNLTQAEQLAQLIKEADALVVGIGAGMSAADGFTY
                     IGPRFETAFPDFIAKYQFLDMLQASLFDFEDWQEYWAFQSRFVALNYLDQPVGQSYLD
                     LKEILETKDYHIITTNADNAFWVAGYDPHNIFHIQGEYGLWQCSQHCHQQTYKDDTVI
                     RQMIAEQKNMKVPGQLIPHCPECEAPFEINKRNEEKGMVEDADFHAQKARYEAFLSEH
                     KEGKVLYLEIGVGHTTPQFIKHPFWKRVSENPNALFVTLNHKHYRIPLSIRRQSLELT
                     EHIAQLISATKTIYQKS"
     gene            15065..16084
                     /locus_tag="VC122_001029"
     CDS             15065..16084
                     /locus_tag="VC122_001029"
                     /EC_number="6.3.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010922342.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoate--protein ligase"
                     /protein_id="HEP5640961.1"
                     /translation="MYLIEPIRNGKRITDGAVALAMQVYVQENLFLDDDILFPYYCDP
                     KVEIGKFQNAVVETNQEYLKEHHIPVVRRDTGGGAVYVDSGAVNICYLINDNGIFGDF
                     KRTYQPAIEALHHLGATEVEMSGRNDLVIDGKKVSGAAMTIANGRVYGGYSLLLDVDF
                     EAMEKALKPNRKKIESKGIRSVRSRVGNIREHLAPQYQGITIEEFKDLMVCQLLQIET
                     ISQAKRYDLTEKDWQQIDALTERKYHNWEWNYGNAPQYRYHRDGRFTGGTVDIHLDIK
                     KGYIAACRIYGDFFGKADIAELEGHLIGTRMEKEDVLATLNAIDLAPYLGAITAEELG
                     DLIFS"
     gene            16212..17882
                     /locus_tag="VC122_001030"
     CDS             16212..17882
                     /locus_tag="VC122_001030"
                     /EC_number="6.3.4.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017646002.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate--tetrahydrofolate ligase"
                     /protein_id="HEP5640962.1"
                     /translation="MKSDIEIAQSVALQPIIDIVKKVGIDGDDIELYGKYKAKLSFEK
                     MKAVEANEPGKLLLVTAINPTPAGEGKSTMSIGLADALNQMGKKTMLALREPSLGPVM
                     GIKGGAAGGGYAQVLPMEDINLHFTGDMHAITTANNALSALIDNHLQQGNDLGIDPRR
                     IIWKRVLDLNDRALRQVIVGLGSPVNGVPREDGFDITVASEIMAILCLATDLKDLKKR
                     LADIVVAYTYDRKPVYVRDLKVEGALTLILKDAIKPNLVQTIYGTPALIHGGPFANIA
                     HGCNSVLATSTALRLADYTVTEAGFGADLGAEKFLNIKVPNLPKAPDAIVIVATLRAL
                     KMHGGVAKSDLAAENCEAVRLGFANLKRHVENMRQFKVPVVVAINEFVADTEAEIATL
                     KALCEEIKVPVELASVWANGAEGGLALAKTVVRVIDQEAADYKRLYSDEDTLEEKVIN
                     IVTQIYGGKAVQFGPKAKTQLKQFTEFGWDKLPVCMAKTQYSFSDNPSLLGAPTDFDI
                     TIREFVPKTGAGFIVGLTGDVMTMPGLPKVPAAMAMDVAENGTALGLF"
     gene            17967..19544
                     /gene="cls"
                     /locus_tag="VC122_001031"
     CDS             17967..19544
                     /gene="cls"
                     /locus_tag="VC122_001031"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002984563.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cardiolipin synthase"
                     /protein_id="HEP5640963.1"
                     /translation="MIIKKKAKVKYLLHKGKHGFLRGIFSRTTIIVLLIILQLVFLFQ
                     SYAWMEQYRVWITILESVFAITIVLYLVNSDMDAISRMTWLILIMIAPLLGSLFLIYT
                     KLDWGYRGLKQRINHLVDLSAPYLSDDDAILEVLKDSTSTTYHLVQYLERSRGNFPIY
                     NNTRVTYFPTGETFFDSLKEQLFLAKKYIFLEFFIIAEGQMWGEILSILEKKVSEGVE
                     VRVLFDGMNELSTLSSDYAKRLEQIGIKAKSFLPISPFISTYYNYRDHRKIVVIDGEV
                     SFTGGINLADEYINEVERFGHWKDAGLMLEGEATDSFLILFLQMWSITEKELIIDPYL
                     SDHSLKLPSDGYVIPYGDSPLDTDKIGKNVYIDILNHAKEYVYIMTPYLILDSEMEHA
                     LRFASERGVDIRIIMPGVPDKGVPYALAKTYYKALMTSGVKIYEYQPGFVHSKVFISD
                     NTKAVVGTINLDYRSLYHHFECATYLYRVSVIADIVNDFNEAQKQSLLMTSDHLTQRP
                     WYQKLIGLLVRTIAPLL"
     gene            19884..20222
                     /locus_tag="VC122_001032"
     CDS             19884..20222
                     /locus_tag="VC122_001032"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018365984.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEP5640964.1"
                     /translation="MQIIKRDGHVADFDPDKIYQAIIKAARTVYVIDDTWRQNLTHVT
                     KKVVIDLEEAHVERPTINMVQSLVENRLMDAGYITIAEHYISYRLQRDLERNGYGDKI
                     VVHLRFEQIK"
BASE COUNT         6301 a         3483 c         4256 g         6527 t
ORIGIN      
        1 tctactcgag taaccttttt taatgtattt taactgaaaa aggctttcat aattttaaaa
       61 aaatgttaaa ataggtgtat aacaactgat taccaagaga ttattatcat tatcttctaa
      121 ggtgtcattg ttatggtgac aatcaaccat acgctactaa agataggtag cgttcgatca
      181 caggttgatc aagtgattga tacagatatt tataatttta caaatatctg tatcaatcca
      241 tgttgaatag tctatgcttc taaggtgtcg acgttaatgt actgacggga ccgttatagt
      301 ctttcttttt ggagaaacgt cggatgccag tgaactagtt gaagttaaag atgttaattc
      361 aaacagtgct gaagagattg atatcgtttt gaacggtcgt ttacttcaaa gcaaagaaat
      421 taccagtcta tcggagatga tataagtgct aaagttagat cacgttaatt atctatcaag
      481 tcgggtgcat atttcgttaa agtatctaca ggggtttcaa tagatgtagc tattatgaaa
      541 ggtgatgtta aatggggact actgatttag taagttgtgc tgtacaagct agtgagtatg
      601 cttatgtccc ttactctcat tttccagtag gagcagcttt aaaaacaaaa gatggaacga
      661 tctatactgg ctgtaatatt gaaaatgtta gttttggtct gacaaattgt ggagagcgaa
      721 cagctatatt taaagccata tctgatggtc ataaagaatt ggttgaaatt gctatttatg
      781 gagaaactat gcaacctgta tctccttgtg gggcttgtcg tcaggtaatg gcagaatttt
      841 ttgctccttc ttctttagtc actttaattg ctaaaaatgg tcaaactgtt gaaatgactg
      901 ttggagattt actcccctat tcttttacag atttaatata atctgcttta atttctgtta
      961 tctagccgtc ttggctttat atttactttc agcaacttat ttgctagaaa tcttttttag
     1021 gagggttcat attcatgaac aagaaattta ttggtcttgg tttagcgtca gtggctgtgc
     1081 tgagtttagc tgcttgtggt aatcgtggtg cttctaaagg tggggcatca ggaaaaactg
     1141 atttaaaagt tgcaatggtt accgatactg gtggtgtaga tgacaaatca ttcaatcaat
     1201 cagcatggga aggcctgcaa tcttggggta aagaaatggg ccttcaaaaa ggaacaggtt
     1261 tcgattattt tcaatctaca agtgaatctg agtatgcaac taatctcgat acagcagttt
     1321 caggagggta tcaactgatt tatggtatcg gctttgcatt gaaagatgct attgctaaag
     1381 cagctggaga taatgaagga gttaagtttg ttattatcga tgatattatc gaaggaaaag
     1441 ataatgtagc cagtgttacc tttgctgacc atgaagctgc ttatcttgca ggaattgcag
     1501 ctgcaaaaac aacaaaaaca aaaacagttg gtttcgtggg cggtatggaa ggaactgtca
     1561 taactcgatt tgaaaaaggt tttgaagcag gagttaagtc tgttgacgat acaatccaag
     1621 ttaaagttga ttatgctgga tcatttggtg acgctgcaaa aggaaaaaca atcgcagcag
     1681 ctcagtatgc agcaggtgct gatgttattt accaggcagc aggaggcact ggagcaggtg
     1741 tatttaatga agcaaaagct attaatgaaa aacgtagtga agctgataaa gtttgggtta
     1801 ttggtgttga ccgtgatcaa aaagacgaag gaaaatacac ttctaaagat ggcaaagaag
     1861 caaactttgt acttgcatca tcaatcaaag aagttggtaa agctgttcag ttaatcaaca
     1921 agcaagtagc agataaaaaa ttccctggag gaaaaacaac tgtctatggt ctaaaagatg
     1981 gcggtgttga aatcgcaact acaaatgttt caaaagaagc tgttaaagct attaaagaag
     2041 cgaaagcaaa aattaaatct ggtgacatta aagttcctga aaaatagtgt ttaagaacaa
     2101 gaagcgcgac ctttgtcggt cgctcttttt aaactgagat aatttttatc tcagtaaagt
     2161 ttgtgatagg tgatagatca taggctttac tgaaataaaa gattgcattg aaaggaaatg
     2221 atcctatgac acagcatgtc attgaaatga gagaaatcac gaaaaaattt ggtgattttg
     2281 ttgcaaatga ccacattaac ttaaatgtca gaaaaggtga gattcatgcc ttattgggtg
     2341 aaaatggggc aggtaaatca actttgatga atatgctagc tggtctctta gagcctacta
     2401 gtggagaaat tgtcattaat gacaagccag ttcaaattga ttcgccatct aaatcagcta
     2461 agcttggtat cggaatggta catcaacatt tcatgttagt agaagccttc acggttgctg
     2521 agaatattat tttaggaaat gaagtagtaa aaaatgggtg cttagatctt aaccaagcat
     2581 cgaaagatat taaggtacta tccgaaaaat atggccttgc aatcaatcca tcagctaaag
     2641 tttctgatat ttcagtagga gcacaacaac gtgttgagat tttgaaaacc ttgtatcgtg
     2701 gtgcagatat tcttatcttt gatgagccaa ctgccgtttt aacacctgca gaaattaaag
     2761 aattgatgac tatcatgaag aatctcgtga aagaaggaaa atcaattatt ttaattaccc
     2821 ataaactgga cgagattcgt gctgtcgccg agcgtgtcac agttattcgt cgtggtaaga
     2881 gtatcgaaac agttgatgtg gcaggagcaa cttcacaaga tttagctgag atgatggtgg
     2941 gacgctcggt ttcctttaca actagcaaaa aagctgctga accaaaagat gtggtcttat
     3001 ccatcaaaaa tcttgaagta gatgaaaata gagggattcc agcagttaag gggctatctc
     3061 tagatgtccg agctggtgaa attgtaggga ttgctggaat tgacggaaat ggtcaatctg
     3121 agttaattca agctattaca ggtttacgta aagtcaaatc tggaagtatt atgattaaaa
     3181 acaaagaagt cactcatttg tcttcacgca aaattactga gttatcagtg ggtcacgtgc
     3241 cagaagatcg tcatcgagat ggtttgatac tagatctttc tttagctgaa aatacagcct
     3301 tacaaaccta ttacaaacaa cctctaagtc aaaatggaat cttaaattat actaaaatta
     3361 atgactatgc tcgtcaatta atgaaagaat ttgatgttcg cggagccaat gagctagtac
     3421 cagctagagg attttctggt ggtaaccaac aaaaggccat tattgctcgt gaagtggacc
     3481 gtgatccaga tttacttatt gttagccaac caacacgtgg tcttgacgta ggagctattg
     3541 aatatatcca taagcgtttg attaaagaac gtgataaagg taaagctgtc ctcgttgtta
     3601 gttttgaact tgatgaaata ttgaatttat ctgatcgaat tgctgttgtt catgatggta
     3661 aaattcaagg tatcgtattg ccagaaaata ccaataaaca agaattgggt attttaatgg
     3721 caggtggttc aattcataag gaggaaggtc atgtctaaaa atgcgcaaaa aattgctgta
     3781 ccattgattt ctgtcttgct aggattttta cttggcgcta ttattatggt tatctttggt
     3841 tatgatccta tctgggggta tgaaggttta ttccaaattg cctttggtag tgttaaaaat
     3901 attggagaaa tattccgttc aatggggcca ttgattttaa tagcgcttgg ctttactgtt
     3961 gctagccgtg caggcttctt taacgttggt ttgtctggac aggctttagc tggatggatt
     4021 tcagcaggtt ggtttgcact cttaaatcca gatatgcctc gtccattatt gattcttatg
     4081 acagctctta tcggaatgat tgctggtggt attgctggag ctattccagg gatacttcgt
     4141 gcttatcttg gtacaagtga agttatcgtt acgattatga tgaactacat cattttatat
     4201 gtcggaaatg ctattgttca acgtggttat cctgagagtg tcaaacaaag tattgattca
     4261 actattcaag ttagtgataa tgctagttat caaacgcatt ggttatcagc cctaactaat
     4321 aactctcgta ttaatattgg tattttcttt gccattattg ccattgccct aatctggttc
     4381 ttgcttaaca aaactacttt aggttttgag attcgttcag ttggtctcaa cccacatgct
     4441 tcagaatacg cagggatgtc atcaaaacgt actatcattt tatcaatgat tatctctgga
     4501 gctcttgcag gtcttggagg agtagttgaa ggtctaggaa cctttgaaaa tgtttttgta
     4561 caaggatctt ctttagcagt tggttttgac ggtatggcag ttagcttatt agcagcaaat
     4621 tcaccgcttg gaatattttt ctcatcattc ctatttggtg tcttaaatat tggtgctcca
     4681 ggaatgaata ttgcaggcat tccaccagaa cttgttaaag tagtaactgc atcaatcatt
     4741 ttctttgttg gttcacatta cctcattgaa cgttacatta ttcgccctaa aaaattagtg
     4801 aaaggtggta aataagatga gtttagtaac aatttttgcc ttattggtgt catctatgct
     4861 tatttatgca acaccactca ttttcacaag tattggagga actttctcag agcgctcagg
     4921 cgttgttaat gttggtttag aaggtatcat ggtcatggga gcgttctctg ggattgtttt
     4981 caacttagag tttgctgaaa catttggaaa agcaacgcct tggatagccg ttttagtcgg
     5041 tggtattgtt ggtttgattt tttctttgat tcacgctgta gcaaccatca actttcgagc
     5101 tgaccacatt gtcagtggta cagtgttgaa cttgttagca ccttcttttg ctgtcttctt
     5161 ggttaaagct atgtacggta agggacaaac agacaacatt caacaatctt ttgggaagtt
     5221 tgatttccca ggtttatcac aaattcctgt gattggtgat attttcttta aaaatactag
     5281 ccttattggt tacttcgcca ttgctttttc gttctttgct tggtttttgc tttataaaac
     5341 caggtttggt ctgcgtttac gatcagttgg ggaacaccct caggcagctg acacacttgg
     5401 tattaacgtc tatttgatga aatactatgg tgtgatgatc tcaggttttc ttggtggaat
     5461 aggtggagct gtttatgctc agtcaatctc cgttaacttt gctgtaacaa ctatcttagg
     5521 tcctggattt attgctttgg cagctatgat ctttggtaaa tggaatccag ttggcgccat
     5581 gctatctagt cttttctttg gcttatcgca aagtttagcc gttattggtg ctcaattacc
     5641 attactagaa aagattccaa cggtttactt acagattgca ccttatatgg taacgattat
     5701 tattttagct gctttctttg gtcaagcagt tgcaccaaaa gctgatggaa tcaactatat
     5761 taaatctaaa taacgttata ttagtagtaa gcctcttagt gaggtttaac agcactccta
     5821 aagcaagata gttaggtcta gctttaaggg tgcttttttg ggacttaccg caagcgtttg
     5881 gattgccagt caccatcaca atagaagcaa gaatagacta aaaagaacta agtcagcggc
     5941 ttagttcttt ttgcttgtct ttcgttttta ctgatagctc ttaagttgtt ttagccaaca
     6001 aaggtattga tttctgtttc aatgttagca attttttctt gagcgatgtc aagagtatca
     6061 cctattgtgg caatatagaa cttgattttt ggttcagttc ctgaagggcg aactgcaatc
     6121 catgaatcat cagctaacgt gtacttcaaa acattacttg gaggagttgt taagttagat
     6181 attgttccat catttttagt agcggtttgt ttttggaaat cttctaataa tacaatatca
     6241 gtattgttaa attgttttgg accattttca cggaatttgt ccataatctt cttaatttca
     6301 gcagctccat ccacacctga aagtgttact gaaatcgttt tttcagcaaa atagccatat
     6361 tctttgtaaa tttcatcaat accatctgca agtgttaaac cacgcgaacg gtaataagct
     6421 gcgatctcag caaccaaaag aactgcttga atagcatctt tatcacggac aaatggttta
     6481 ataagatatc cgaagctttc ttcaaaacca aacatatagg tgtggttatg tttttcttca
     6541 aactcttgaa ttttctcagc gatgaatttg aatccagtta aaacgttaaa cattgtagcg
     6601 ccataacttt cggcaatttt tgttactaat tctgttgata cgattgattt agcgagagca
     6661 gcattttctg gaagtgttcc tgctgtttta tgagcttcca ggatgtattt cgcaataatc
     6721 gctccaattt gatttccaga aaggttttta taagaaccat ctggttggcg aatttcaacg
     6781 cctaggcggt ctgcatcagg atcagtagct actaagacat ctgcatcaac ttctcgtccg
     6841 agttcttcag caagagcaaa ggcagcttgg ctttcagggt taggcgattt aacagtagaa
     6901 aagtctggat ctgccttagc ttgtgattcg actacaacaa cagattcaaa accggcttga
     6961 gcaagggcac ggcgtgtcaa catctcacca gttccgtgga gaggagtata gacaattttc
     7021 atgtcttttc caaagttatt gattaaatct tgattgatat taacatcttt aacctctcta
     7081 agatattcca tatcaacatc ttcaccgata acttgaataa gacctgatga tttgctttca
     7141 tctaaatcag caagttcaac tgcaaatgga ttttcaatag cacggataaa gtctgttagt
     7201 gcatcagcat cagctggtgg tagttgacct ccgtcttgtc catagacttt ataaccattg
     7261 aaaggtgctg gattgtggct tgcagtaacc ataataccag cataggcatt taaatggcga
     7321 acagcaaaag agagttctgg tgttggacga agagcttcaa atacgtaaga tttaatccca
     7381 tgctgtgcca agacctgtgc agactcaaaa gcaaattctg gagagaagtg gcgcgaatca
     7441 taagcgatgg ctacaccatg ttttttagct tcctctccct tagtttcgat taattttgct
     7501 aatccttcgg ttgcttgacg gacaacataa atattaatac gatttgtacc agcaccaata
     7561 taaccacgca taccagcagt accaaattct aagtttgtgt aaaaagcatc ttcttttgtt
     7621 ttttcatcca ttgatagtaa ttcttgacga agatagtcag gaagctgctc aaaatctaac
     7681 catttttgaa aattttctgt gtaagtcatt gaaatactcc ttcaatattt ataaacgctt
     7741 tcattatatc attttgaagg aaaaaaaaca ctaattattt gcagctgttt acaaaacttt
     7801 ttccatattt tcaagtagct aatgtaagaa agttaaaata accaagtttt taaaagagaa
     7861 aaactcggtt attgtgaaag ctaattacct ttttagtctt gatagtgttg gaataagtac
     7921 aaaggtaata atagctgaga tgatcatctc aacaatagcg tttgatgaga tgatggctgt
     7981 tagtagtgct ttaatatttc catcaaaaac tgtcgcgaaa aaaataaata tcccagataa
     8041 tacaaatata gtatttgtta gagaaccaat gataccggat actattaaac caaactgatt
     8101 aggaatcaat ttatagcaat aataaggggt aataccaatc aaaatacgtg ggataatagc
     8161 aattattaaa gaagcgaaag ttccatgatc gacaaaaggt gaaaagagat aatttgtagg
     8221 cagtagaatg atggtatttg taatgacact tataatcccc attaatcctc ctaaaatagc
     8281 acctatcctt gggccatata agacagaagc aataataact gggatatgga caagcgttgg
     8341 tttgattgga ataggccaaa tattaaagac aagactgctc acaaaatgaa tgactaacat
     8401 gatggcaaaa aagatagcaa tacgagaaat atctgctgat ttacggtgat tcattttact
     8461 ctccaaaaaa tataatactt aattctaaaa aggtgatcag aaaaacgact gttaaagttt
     8521 ccaaatcgta tcaatagtag ctaagataac atcaatatca gccaaagcac caggtccttt
     8581 atctccacag gctaataagc tagattttgg aggaatttct gtaaacccta ttgtcgataa
     8641 gcgtttaata ttttcttggg taatagggtt ttgatacatt ttggtattca tggctggggc
     8701 tattaatttt ggtgtggtag ctggtagggc aagagcaaca cttgttacaa tattatcagc
     8761 gaatccatat gctagatgag caatagtatt agcagaggca ggtgcaacaa taaataaatc
     8821 tgtgcgtttt gctaattcga tatggttgat aacttttggg tcatgctcat ccataacatc
     8881 taaatgaata gcattttttg ataacacttg cagggttaat ggtgtaataa attgtgtagc
     8941 agcctgagtc attatgatgt gaacatcata gccaatttta gttaattgac tcgtaagatc
     9001 tgctgcttta taggcagata tactaccaga aacagctaag gtaatatgtt tagtcatgtt
     9061 tggtcatcct ttcatataat aagtcagcaa ttgcctcttt ggtatctgca gatgcaactt
     9121 cgttattgga tatcaaaaga gccttgtgca tacctgtttg gatatcaact aaatcattgg
     9181 cgagaatata atcagcatgg tttttagcta gacttgctct ggctacttta attaactctt
     9241 cttgaggcac attaactaga agtttaaagc cgactagttt aatattagga ttccactgtt
     9301 taacataaga gataactttg ggtgtttttt tcaaaaaaag aacctgataa tcagatgcag
     9361 atgagatttt cggctcactg ttatgttcac atagaaaaca attaaggtta tcagcttgag
     9421 agacttgttc caaatcagtc atgtagactg gagtatagtc agaaacagcc atactatgaa
     9481 ttagaatgtc atggtgagga acctgatctt tgagggcagc cattaagtcg ttaactgttt
     9541 ccacttcaat aatccttagg agcttatttg gcaggggttt agtggctgtt ttagtagtga
     9601 ctagggtaac gtcatgatga tattgtaaaa atcgctctgt gattaatttt ccaagttgcc
     9661 cagttgagtg attagtgata cctcgtactg catctatagg ttcagttgtt ccacctgatg
     9721 ttattattaa tttcatagtc atattttatc aaaaaaggag ctgatagtaa acggaagata
     9781 atgacgccag acattgtctt gcgtttgtat taatatggaa tagtaataga tttctagtaa
     9841 ttaaaacgaa gttctggaaa gcaagacact atagtagcta aaatctaaat tttaaggtaa
     9901 aatagagcaa gcggtttcaa gtgtgaaagg aagaattgtg ataactatta gagatttagc
     9961 gtcactgccg atttttgttt acggtgatgg aaataaaaaa gttccaggag ctttgagtcc
    10021 ttttgtgtgg acagaggtgt ttttaaaaac tatcaatcaa gaacctaatc aactgatctt
    10081 acatatttgg ccgatgacaa gaacagttat attagggatg ttagatagac agcttcccta
    10141 ctttgagctg gctaaaacag agattggcaa taatggttat gttcctgtta ctcgtaatat
    10201 tggcggtttg gctgttgtcg ctgatgatgg gattttgaat ttttcgcttg ttattccaga
    10261 ccatttttcg gaatctatca gtattagtaa tgcttattta ataatggttg atgtcattag
    10321 agagagtttt tcggattatt atcagcgtat tgaatatcac gaaattaaaa attcttattg
    10381 ccctggtaat tttgatctta gtattgcagg ccgcaagttt gcaggtatcg cccagcgtcg
    10441 tatcaaaaag ggaattgttg tgtctattta cttgagcgtt tgtggggatc aagcagcgcg
    10501 tggtcaactc atcaaggatt tttatgaggc cggaacacaa ggagaagtta ccaaggttaa
    10561 ttaccctcaa attgatccag aatgcatggc aactctttca gaactccttg agacgccttt
    10621 tacagtggca gaagtccttg aacgtcttcg attgaccttg cgacagcttg gcttttccct
    10681 cacggaaaaa agtccagatc aagccttact gactaatttt gatgctgtct atgaacggat
    10741 gcagctagaa gtcgtcagaa aggaaggcaa atgaaagaaa gatttagtcc tttatttgag
    10801 cctttgactt tgccaaatgg gagtcaattg gataatcgct ttgttttgtc tcctatggtg
    10861 accaactcat ccacaaaaga cggttatgtc acccaagatg atgtatccta tgctttgaga
    10921 cgagcagctt ctgcccctct acaaattaca ggagcggcct atgtggatcc ttatggtcaa
    10981 ttgtttgaat atggttttag tgttacaaaa gatgccgata tttctggctt gaaagagcta
    11041 gctcaagcga tgaaggcaaa gggagctaaa gctgtcttac aactgaccca cgctggccgg
    11101 tttgctagcc atgccttgac taaatatggc tttgtctatg ggcctagtta catgcagttg
    11161 agatctcccc aacctcatga agtcaaacct ttaacaggtc agcaaataga ggaattgatt
    11221 gcagcttacg ctcaggccac tcggcgggca attcaagcag gctttgatgg cgtggaagta
    11281 tcttcggcgc agcgcttatt gattcaaacg ttcttttcga ctttttcgaa caagaggaca
    11341 gatagttatg gttgtcaaac actttttaat cgctctaagt taactttagc tgttttgcaa
    11401 gctgttcaac aggtgattaa tcaagaagct cctgatgggt ttatttttgg atttcgtgct
    11461 acaccagaag aaactagggg aaatgatatt ggttatagta ttgacgagtt tttgcagttg
    11521 atggattggg tacttaatat tgcaaaactt gattatttag cgatagcaag ttggggccgt
    11581 catgtatttc gaaatactgt tcgttctcct ggcccttatt atggaaggcg agttaatcag
    11641 gtagttagag attatttaag aaataagcta cctgttatgg ctacaggggg aatgaacaca
    11701 cctgacaaag ctattgaagc actagctcat gctgatttta ttggagtatc tacacctttt
    11761 gtcgtggatc cagaatttgc tcataaaatc aaagaagggt gtgaagagag tattcattta
    11821 aggattcgac ctgctgattt gaaaagttta gcgattcctc aggcttcttt caaagatatt
    11881 gtacctttga tggactacgg ggaatcattg cctaaagaat caagaacttt atttcgtagt
    11941 cttactcaca attataagga gattaaatga aagtcagtat tcttgattat ggggtgattg
    12001 ataaagaaaa aacaccccaa gaagctttat tagaaacgcg ctgcttagca caagtagccg
    12061 acaaacttgg ttttcatcgt ttttgggtag cagagcatca taatatttat gcctttgcaa
    12121 taagcagtcc agagctgttg atgatgcatt tagcagatca taccaaacag attcggatag
    12181 gttcaggagg tattatgcct ctacattata gtagctttaa gattgcagaa tggataatga
    12241 ctttagaggc cttgcaccct aatcggattg atttaggtat tggcaactct ttaggaacta
    12301 cgttagttca aagagctcta tcaagtatcc attgcaaaga cagctatagt caggttgtca
    12361 cagaattata ccagtatctt aatcctgatc atttgtcacc actaccgatt tttgttaacc
    12421 ctaggggtaa tacttaccct cagatttgga cgctaagcaa tagtttggag acagcagaac
    12481 tggctggtca attaggttta ggctatacct ttggtatttt cccctatata ccaaaagatc
    12541 ctataacaga agctaaaaga gtatcggcac attatagaaa ggcttttagg ccttcaaagc
    12601 tattaaaaat ccccaagtta atattagcag tttttattgt cctttctgat accgacgagg
    12661 aagcagaagc tttggctaaa ccgttggaca tttggatgct tggtcagcaa gattttaatg
    12721 aatttaagac ctaccctgat gttgaggagg ctagaaacta tcacttaaca gagaaacaaa
    12781 gagaagccat tgcagcaaat cgtagtcgca tggttattgg atcacctcac acagtcaaga
    12841 aacaattgga taggctgata gaagcttgtc aagctgacga attattagcc atccccttag
    12901 ttcctgaatt tgccaataga caacgaacct tagagctgct agcagactta tacgtgacca
    12961 tttaacctta ccaaaaacaa ttccctaaac tgaaaggaaa gaaacaatga aaaaaattgc
    13021 aaattactta ttaattgaga aaacagatga ccgctatacc attagcatga caccagaatt
    13081 acaagacgat attggtacta ttggttacgc tgaatttacg gataacgacc accttgccgt
    13141 tgatgacatt atcttaaatc tagaggcttc taagaccgtc atgtctgtct tgtcaccatt
    13201 ggctggggca gttgttgagc gcaatgaggc agccacacta acaccaaccc ttttgaattc
    13261 agaaaaagct gaggaaaatt ggattgttgt gctaacagat gttgatcaag cagcttttga
    13321 tgccttagaa gatgcctagt tcatttgatt tattagggga aatgattgat ctgttgcaaa
    13381 cagagcagct tactagttct tgggcctgtc ccttgcctaa tgctttaaca aagcgacaag
    13441 acttgtggcg ggcgctcatt aaccagcgcc ctgctttgcc tttatcaaaa gattacttga
    13501 atctggaaga tgcttatcta gatgattggc gtgcttcttt tgttcctgta tcagtcaagg
    13561 actgccaaaa aactaattat accagtcttt ttctttacca tggggatatt aggtaccttg
    13621 ctgtggatgc tattgtcaat gctgctaata gtgagttatt gggatgtttt atccctaacc
    13681 atggctgtat tgataatgcg attcatacgt ttgccggaag tcgtttacgc ttagcttgtc
    13741 aggcaatcat gactgaacaa ggccgtaagg aagccattgg ccaagcaaag cttaccagtg
    13801 cttatcatct accagcctct tatattattc ataccgtagg gccaagaatt accaaaggac
    13861 gacacgtttc tccgattcga gctgatttgt tagcacgttg ttatcgttct agtcttgatt
    13921 tggcggttaa agcaggattg actagcctag ctttttgctc tatttcaaca ggagaatttg
    13981 gctttcctaa aaaagaagcc gctcagattg ccattaagac agttttaaaa tggcaagcag
    14041 aacatccaga atctaagact ttaactatca tttttaatac ctttacatca gaagacaagg
    14101 ccttatacga cacttatttg caaaaggaga ataactgtga gtaattggac aacctatcct
    14161 cagaaaaatc tcacccaggc agaacaactg gcccaattaa tcaaagaagc agatgcactt
    14221 gtagttggta ttggagctgg catgtctgca gctgatggtt ttacttatat cgggccgcgt
    14281 tttgagacgg cattcccaga ttttattgct aagtatcagt ttttggatat gctgcaagct
    14341 agcctttttg attttgaaga ttggcaagaa tattgggctt ttcaaagtcg ctttgtcgct
    14401 ttgaattatc ttgatcagcc cgtaggccag tcttatcttg atttaaaaga gattttggaa
    14461 accaaagact atcatattat tactactaat gctgataatg ctttttgggt ggctggttat
    14521 gatcctcata acatttttca cattcaagga gaatatggtt tgtggcaatg tagccagcac
    14581 tgccatcagc aaacttataa ggacgatacg gtcatccgtc aaatgatcgc agagcaaaaa
    14641 aacatgaaag tcccaggtca actgattcct cattgccctg aatgtgaggc gccttttgaa
    14701 atcaataagc gtaatgaaga aaaaggaatg gttgaagatg ctgactttca tgcccaaaaa
    14761 gcacgttatg aggctttttt atcagaacat aaagagggta aagtgcttta tctagaaatt
    14821 ggtgtgggac atacgacgcc tcagtttatc aaacacccct tttggaaacg tgtctcagaa
    14881 aaccctaatg ccttattcgt taccttgaat cacaaacatt accgcatccc tctgtcaata
    14941 aggcgacaaa gcctagaact gacagaacac attgcacaat tgattagcgc cacaaaaacc
    15001 atttaccaaa aatcgtaacc aaagccaata gacggttaat taagagatat agaaggagac
    15061 taacatgtac cttattgaac cgattcgtaa cggtaaacgt atcaccgatg gagctgttgc
    15121 tctagccatg caagtttatg ttcaagaaaa tctattttta gatgatgata ttttatttcc
    15181 ctactattgt gatcccaaag ttgaaattgg aaaattccag aatgcggttg tagaaactaa
    15241 tcaagagtat ttaaaagagc atcatatccc tgttgttcgc cgagatactg gcggtggtgc
    15301 cgtttatgta gattctggtg ctgttaatat ttgctacttg attaatgata atggtatttt
    15361 tggtgatttt aaaagaacct atcagcccgc tattgaagcc cttcatcatt tgggagcaac
    15421 agaggttgaa atgtctggac ggaatgactt ggttattgat gggaaaaagg tatctggcgc
    15481 tgcgatgact attgctaatg gccgtgttta tggggggtat tcccttttgc tagatgtgga
    15541 ctttgaagcc atggaaaaag cattaaaacc taatcgcaaa aaaattgaat caaaaggcat
    15601 caggtcagtt cgtagtcgcg ttggaaacat cagagaacat ttagcaccac aataccaagg
    15661 tattacgatt gaagagttca aagatttaat ggtttgccaa ttgttacaga ttgagacgat
    15721 tagccaagct aagcgttatg atttaacgga aaaagactgg caacaaattg atgctttaac
    15781 agagcgtaaa tatcacaatt gggaatggaa ttatggcaac gcgcctcaat accgttacca
    15841 tagagatggc cgttttaccg gtggaacagt agatattcat ttagatatta aaaaaggcta
    15901 tattgcagct tgtcgcattt acggagattt ttttggcaag gcagacattg cagaacttga
    15961 aggtcacttg ataggaacac gaatggaaaa agaggatgtc ttagctaccc tcaatgcaat
    16021 tgatttagcc ccttatttag gtgcgattac agcagaagaa ttaggagatt tgatattttc
    16081 ttgataaaaa agatacctca gaccggtaaa gtgcgattaa aaacgaacaa taattgtttt
    16141 aattcaaata aatgtatgta tgatatgaat tgtgatataa tgacgtcatt acgtaaaatt
    16201 gaggagtcat catgaaatca gatatcgaaa ttgcccaaag tgtcgcccta caaccaatca
    16261 ttgacattgt taaaaaggtt ggaatagacg gcgatgacat tgagctttat ggcaaatata
    16321 aggctaaatt gtcttttgag aaaatgaaag ctgtcgaagc taatgagcct ggaaaattac
    16381 tcttggtaac tgctattaac cctactccag cgggagaagg aaaatcaacc atgtctattg
    16441 gactggcaga tgccttaaat caaatgggaa aaaaaaccat gcttgcctta cgtgaaccct
    16501 cccttggtcc tgttatgggg attaaaggcg gggcagcagg tggtggttat gcccaagtat
    16561 tgcctatgga agacattaat cttcacttta ctggtgacat gcatgccatt acaaccgcta
    16621 ataatgcatt atctgctttg attgacaatc atttgcagca aggaaacgat ttaggaattg
    16681 atcctcgccg cattatttgg aagcgcgtgt tggaccttaa tgatcgtgct cttcgtcagg
    16741 taattgttgg cttaggcagt ccagtgaatg gtgtgccacg cgaagatggg tttgatatca
    16801 cagtagcttc tgaaatcatg gcaattttat gtttggcaac tgacttgaaa gatctaaaga
    16861 aacgtcttgc tgatatcgtc gtggcttata cctatgatcg taagcctgtc tatgtgcgtg
    16921 acttgaaggt tgaaggagca ttgacattaa ttctaaaaga tgctatcaag ccaaacttgg
    16981 tgcagactat ttatggaaca ccagctctta ttcatggggg gccatttgct aatattgctc
    17041 atggttgtaa ttctgtcctt gctacctcaa cagctttgcg attagcagat tacactgtta
    17101 ccgaagcagg attcggggcc gacctaggag ctgaaaaatt cctaaatatc aaagtaccta
    17161 atcttcctaa agcaccagat gctatcgtca ttgttgcaac cttgcgcgcc cttaaaatgc
    17221 atggaggtgt cgccaaatca gatttagccg ctgaaaattg tgaggctgtt cgcttaggat
    17281 ttgccaactt aaaacgtcac gtggaaaaca tgcgccaatt caaagttcca gttgtggtgg
    17341 ctatcaatga atttgtagca gataccgagg ctgaaattgc taccttaaaa gcgctttgtg
    17401 aagagattaa ggttccagta gaattagcca gtgtttgggc aaatggagct gagggaggct
    17461 tagcattagc taaaacagtg gttcgtgtga ttgatcaaga agctgctgac tacaaacgtc
    17521 tctattcaga tgaggatact ttggaagaaa aagtgatcaa tatcgttacc cagatttatg
    17581 gtggcaaagc tgttcaattt ggccccaagg ccaaaacaca attgaagcag ttcacagaat
    17641 tcggttggga taaattgcct gtttgtatgg ctaagaccca atacagtttt tcagataacc
    17701 catccttgct tggagcgcca accgattttg atattaccat tcgtgaattt gttcctaaaa
    17761 caggggcagg ttttattgtt ggtttaacag gtgatgtgat gaccatgcct ggtttgccta
    17821 aagttccagc tgccatggca atggacgtag cagaaaatgg cacagccctc ggtctctttt
    17881 aggaaaaatt gctcgaataa ttcgagcaag cgctcctttc ttcccgcttt actgtgatat
    17941 aataaggtaa ctgagatttt ggagggatga ttatcaaaaa gaaagcaaaa gtaaaatatt
    18001 tgctccataa agggaaacat ggatttttaa gaggaatttt tagtagaaca acaatcattg
    18061 tattattaat tattttgcaa ttagttttct tatttcaatc ttatgcctgg atggagcagt
    18121 accgtgtttg gataacgatt cttgaaagtg tctttgctat tactattgtt ttatatttag
    18181 ttaatagtga tatggatgct atttcaagaa tgacgtggtt aattttgatt atgattgctc
    18241 cattactagg gtcactgttt ttaatttata caaaattgga ttggggttac agagggctaa
    18301 aacaaagaat aaatcatctt gtagatctgt ctgcacctta ccttagcgat gatgacgcta
    18361 tattagaggt attaaaggat agcacatcaa ccacatatca tttagtacag tacttagaaa
    18421 gaagtcgtgg caactttccg atttataata atacaagggt aacttacttc ccaacaggtg
    18481 aaactttttt tgatagttta aaagaacagt tattcttagc taaaaagtac atttttcttg
    18541 aattttttat tattgcagaa ggccaaatgt ggggagaaat ccttagtatt ctagaaaaaa
    18601 aagtcagtga aggtgtagaa gttagggtgt tgtttgatgg catgaacgaa ctatcaacgc
    18661 tatcatcaga ttacgccaag agattagaac aaatagggat taaagctaaa tcatttttac
    18721 cgatttcacc ctttatctct acctattaca attatcgaga tcaccgaaaa attgtcgtta
    18781 ttgatgggga ggtatcattc actggaggta ttaatctagc agatgagtac attaatgaag
    18841 tagagcgttt tggccactgg aaagatgctg gtttaatgct tgagggtgaa gcaacagaca
    18901 gctttttaat tttgttttta caaatgtggt ctatcacaga aaaagaactg attattgatc
    18961 cttatctttc agatcattct ttaaaacttc cttcagatgg ctatgttatt ccctacggtg
    19021 attccccgct tgatactgat aaaataggta aaaatgttta tatagacatt ttaaatcatg
    19081 ctaaagagta cgtttatatc atgacacctt accttatttt agatagcgag atggaacacg
    19141 ctttacgatt tgcatcagaa cgtggagtag atatccgaat tattatgcca ggagtgccag
    19201 ataagggggt accatatgcc ttagctaaaa cctattataa agctttgatg acttctggag
    19261 taaaaattta cgagtatcaa ccagggttcg ttcattcaaa ggtatttatc tctgataata
    19321 caaaagcagt tgtcggaaca attaacttag actatcgtag cctttatcat cattttgaat
    19381 gcgcgactta tttatatcgt gtgtcagtta ttgctgatat tgtgaatgac tttaatgaag
    19441 ctcaaaagca atcactattg atgacatcag atcatttgac gcaacgtcct tggtatcaaa
    19501 aattgatagg attattagta cgaacaattg cccctctctt gtaattttcg taaaatatgt
    19561 tataatatct gtatgcgatg agtcgattgt gactgcaaag tcacttttgc gcaggggagg
    19621 tcattaaggc gggaagccga ctttgataat ctgtgtgaac cattttatca caatcttatt
    19681 tattaagaat gccccttgga ttgagatatt ctcaatcctt tttttaattt tattatctta
    19741 gttaaaattc agaatgtatc agtgattact gtgtttcaaa ataagaaagt agtttttcgg
    19801 gtatgagtgc tatgacttga caaatgctaa taacatttgc ataatgtaag tagagatgaa
    19861 aacttatgaa aagagatgat cttatgcaaa ttattaagcg tgatggccac gttgctgatt
    19921 ttgatccaga taaaatttat caagcaatta ttaaagcagc aaggacggtc tatgttattg
    19981 atgacacgtg gcgacaaaac ttaacacatg tcactaaaaa agtagttatt gatttagaag
    20041 aagcacatgt agaacgtcct actatcaata tggtccaatc ccttgtggag aatcgtttga
    20101 tggatgctgg atatattacc attgcagaac attacatttc ttatcgtctt cagcgagatt
    20161 tagagcgcaa tggctatggt gataagattg tggttcactt gcgttttgaa caaataaaat
    20221 aaaaacgctt ctaatggcat tttagtgtct ttgaggggat aaggtttaaa tacctaacag
    20281 ataaaaatct gttaggtatt tttttgctac tattaggtgt attctatatc acatagaact
    20341 taagcattca agctttaatt taactttctg tttatttttt gctacatttt gggaaactcc
    20401 cctcctttct tagttgaggt gaataaactt ttatttgata aatcatattt ttaagtaatc
    20461 taaaattatg tttttttaat ttcaaaatca tgctttcgtt tgttataaaa accgatcttc
    20521 catagttaga ttttttatcc aggcaattga agattggggc aactatt
//
LOCUS       DATXIG010000027        20349 bp    DNA     linear   BCT 28-DEC-2023
DEFINITION  TPA_asm: Streptococcus pyogenes strain MGAS11113
            SAMN08555799-rid19682583.denovo.027, whole genome shotgun sequence.
ACCESSION   DATXIG010000027 DATXIG010000000
VERSION     DATXIG010000027.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN08555799
            Sequence Read Archive: SRR6740495
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pyogenes
  ORGANISM  Streptococcus pyogenes
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 20349)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 20349)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-OCT-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-OCT-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001918058.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001918058.2
            Genome Coverage       :: 368x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/12/2023 11:23:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 1,806
            CDSs (total)                      :: 1,769
            Genes (coding)                    :: 1,722
            CDSs (with protein)               :: 1,722
            Genes (RNA)                       :: 37
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1, 1 (5S, 23S)
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 47
            CDSs (without protein)            :: 47
            Pseudo Genes (ambiguous residues) :: 0 of 47
            Pseudo Genes (frameshifted)       :: 30 of 47
            Pseudo Genes (incomplete)         :: 20 of 47
            Pseudo Genes (internal stop)      :: 9 of 47
            Pseudo Genes (multiple problems)  :: 11 of 47
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..20349
                     /organism="Streptococcus pyogenes"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN08555799-rid19682583.denovo.027"
                     /strain="MGAS11113"
                     /serotype="Emm28"
                     /isolation_source="normally sterile site"
                     /host="Homo sapiens"
                     /db_xref="taxon:1314"
                     /country="Finland"
                     /collection_date="2002"
                     /collected_by="National Public Health Laboratory"
     gene            complement(<1..820)
                     /locus_tag="VC122_001033"
     CDS             complement(<1..820)
                     /locus_tag="VC122_001033"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002992583.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HEP5640965.1"
                     /translation="MVTIKDVAQKAGVNPSTVSRVLKDNRSISMKTKEKVRKAMADLG
                     YVPNVAAQILASGLTHNIGLVFPPITTSDRLSEPFFMEILSTITNEAKESHFTVSIAT
                     GISLDDLIEQVKLMHLQKRVDGFIILYSDQDDPVKKYLMTNNLPFVIVGAPQGDTNKT
                     TYIDNDNQLMAKTAVEHLYQKGHHNILFITDDLLSEVTSERYLGYLKGCFKRSLKTKP
                     MLLFDRKDPVSVEILMETISSFKATALVVVGDVLAIRMVQLLSFYDIKVPDDMSI"
     gene            1063..2310
                     /locus_tag="VC122_001034"
     CDS             1063..2310
                     /locus_tag="VC122_001034"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011528703.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="extracellular solute-binding protein"
                     /protein_id="HEP5640966.1"
                     /translation="MKSWQKVIVGGASLTLASTLLVGCGSGSKDKKEAGADSKTIKLW
                     VPTGSKKSYADTIAKFEKDSGYTVKVVESEDPKAQEKIKKDASTAADVFSLPHDQLGQ
                     LVESGTIQEVPEKYNKEIAATSTDQALVGAQYKGKTYAFPFGIESQILFYNKSKLAAE
                     DVTSYDTITTKATFGGTFKQANTYATGPLFMSVGNTLFGENGEDVKGTNWGNEKGAAV
                     LKWIADQASNKGFVSLDANNVMSKFGDGSVASFESGPWDYEAAQKAIGKENLGVAIYP
                     KVTIGGETVQQKAFLGVKLYAVNQAPAKGDTKRIAASYKLASYLTNAESQENQFKTRN
                     IVPANKEVQSSEAVQSNELAKTVITMGSSSDYTVVMPKLSQMGTFWTESAAILSDAFN
                     GKIKESDYLTKLQQFDKDIAATK"
     gene            2584..3945
                     /locus_tag="VC122_001035"
     CDS             2584..3945
                     /locus_tag="VC122_001035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002262977.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HEP5640967.1"
                     /translation="MTQSNLTPNISVIEALKRGSWDIKLSSIIMGFANFANKQFIKGI
                     LFLISELIFLVAFVSQIIPAIRGLVTLGTQTQGMTTKTIDGINIQVAVDGDNSMLMLI
                     FGLASLIFCLVFAYIYWCNLKSARNLYLFKQKGQKIPNFKEDLATLTNGRFHMTLMAI
                     PLIGVLLFTILPLIYMICLAFTNFDHNHLPPKSLFDWVGLANFGNVLSGRMAGTFFPI
                     FSWTLIWAVFATVTNFFFGIILALLINAKGLKWKKMWRTIFVITIAVPQFISLLIMRN
                     LLNDEGPLNALLNKIGLINGSLPFLSDPLWAKFSIIFVNMWIGIPFTMLIATGIIMNL
                     PSEQIEAAEIDGASKFQVFKSITFPQILLIMTPNLIQQFIGNINNFNVIYLLTGGGPT
                     NSEYYQAGTTDLLVTWLYKLTVTAADYNLASVIGILIFTVSAIFSLLAYTRTASYKEG
                     AAK"
     gene            3945..4781
                     /locus_tag="VC122_001036"
     CDS             3945..4781
                     /locus_tag="VC122_001036"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001035402.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HEP5640968.1"
                     /translation="MKNKRRFQLGLVYATLIILSIIWLFPIAWVILTSFRSEGTAYVN
                     YFIPKTFTLNHYINLFTNETFPFGKWFMNTLIVATFTCIISTFITVAIAYSLSRIKFK
                     FRNGFLKLALILNMFPGFMSMIAIYYILKALGLTQTLTALVLVYSSGAALGFYIAKGF
                     FDTIPYSLDESAMIDGATRMDIFFKITLPLAKPIIVYTALLAFMGPWIDFIFAQVILG
                     DATSKYTVAIGLFSMLQPDTINNWFMAFTAGSVLIAVPITLLFMFMQKYYVEGITGGS
                     VK"
     gene            4939..5736
                     /locus_tag="VC122_001037"
     CDS             4939..5736
                     /locus_tag="VC122_001037"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014612372.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HEP5640969.1"
                     /translation="MTSKITIKDIAREAGVSVATVSYVLNQRNDQKISEETRNKVLHI
                     SNLLNYTPNQAARALVTQQSNSVVLYYKYADSILARAEQLHTISFLSRLLHEQDYQLV
                     CLNEDSIEKFDWADLILTLDVDNDTFYKIGKLNFKPLIAIDSFVNDPLFFQINTDYAA
                     LKDQANQFFKDEPYTLLSLPITNRERYEHVSAFFPSLHIVNDLRELTYFEKENLLLID
                     ESLDQFFVNSSQKLYCPRFSPKKGQVILEAIEWAKKRQPDKKHNLLV"
     gene            complement(5852..6667)
                     /locus_tag="VC122_001038"
     CDS             complement(5852..6667)
                     /locus_tag="VC122_001038"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003060440.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1189 domain-containing protein"
                     /protein_id="HEP5640970.1"
                     /translation="MPDKKQFPISYISHCLSARAMFASRAQFTWWQNLLIIVFLNALI
                     MIPVTLHYANMTTYPLERIVTKSLSPITDKTYQALTQGKIEKDTFQGQSLIRRDGELV
                     LAVLPTKVDLEQLASESTRQIIVTKKEWRFVTPDGKELRAHVRGQQQSLADLTTVKAV
                     KDFVNQQWYDSNKASVLGFLLLTFVLMVCVGTLIVIGLGAFFLTLTKRSRLFMIRNFS
                     EGLGLMVNCLAWPSLLAIALSFFIQDPVLIMNCQVFGTLLMLTWVFYKTQFRD"
     gene            complement(6689..7549)
                     /locus_tag="VC122_001039"
     CDS             complement(6689..7549)
                     /locus_tag="VC122_001039"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012767151.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HEP5640971.1"
                     /translation="MRKTYVSISMKRKRFFTQLLTYLYLISLVIVILFPILVTVSSAF
                     RPGNTTAFSFHFDGPWTLSNFKTLFQDTLYLRWYWNTLIVAFFTMLIQVTVITLTGYA
                     YSRYNFFGRKKSLIFFLVVQMVPTMAALTAYFVMAWLFNALNQYWFLILIYVGGGIPM
                     NAWLMKGYFDTVPYDLDESAKLDGSGHFRTFYQIVLPLVRPMIAVQSLWAFMGPFGDF
                     MLAKFLLRAQENYTVAVGLQSFITNDARNPKVTLFAAGAILIAVPISVLFFFLQKNFV
                     SGLTSGGTKG"
     gene            complement(7546..8853)
                     /locus_tag="VC122_001040"
     CDS             complement(7546..8853)
                     /locus_tag="VC122_001040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010922387.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HEP5640972.1"
                     /translation="MVDNQRQQKPNKAMWLSVIPGLGQLYNKQIVKGGVLLVVFFLEL
                     LEIVVLGIPALTGFYSLGSVPMQDHSLFMLIKGAMQLITLVLFGIFHLVAMRDAKLVA
                     HQMNEGKKVPVTAKETLEAIYEKGFPYLLIIPAYLAMAFAIIFPVLVTLLIAFTNYDF
                     RHIPPYRLLDWVGLKNFLNILELSTFKTAFTSVLSWTLIWTLAATSLQIVIGIATAVI
                     ANQSFIRGKRLFGVIFLLPWAVPAFISIMSFGNFFNDSIGAMNVQVLPFIEKFLPFVD
                     FGIVSWKTDPFWTKVAVIMVQGWLGFPYIYILVSGILQAIPADLYEAATVDGATVLQK
                     FRHITMPMIFAVAAPTFISQYTFNFNNFSIIYLFNNGGPGTVGGGAGTTDILISWIYK
                     LTTQTSPQFSMASAVTLIISVIVISVSLITFKKFKAFDMEERS"
     gene            complement(8928..11063)
                     /locus_tag="VC122_001041"
     CDS             complement(8928..11063)
                     /locus_tag="VC122_001041"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010922388.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cyclomaltodextrin glucanotransferase"
                     /protein_id="HEP5640973.1"
                     /translation="MRELHIKTYKLLTKSAVLLGLISFPLTVSAADNASVTNKADFST
                     DTIYQIVTDRFNDGNTSNNGKTDVFDKNDLKKYHGGDWQGIIAKIKDGYLTDMGISAI
                     WISSPVENIDSIDPSNGSAAYHGYWAKDFFKTNQHFGTEADFQQLVKVAHQHHIKVVI
                     DFAPNHTSTAEKEGTTFKEDGALYKNGKLVGKFSDDKDKIFNHESWTDFSTYENSIYH
                     SMYGLADLNNINPKVDQYMKEAIDKWLDLGVDGIRVDAVKHMSQGWQKNWLSHIYEKH
                     NVFVFGEWFSGHTDDDYDMTTFANNSGMGLLDFRFANAIRQLYTGFSTFTMRDFYKVL
                     ENRDQVTNEVTDQVTFIDNHDMERFATKVANNQTAVNQAYALLLTSRGVPNIYYGTEQ
                     YATGDKDPNNRGDMPSFNKESQAYKVISKLAPLRKQNQALAYGTTEQRWISDHVLVFE
                     RKFGNHVALVAINRDQTNGYTITNAKTALPQNSYKDKLEGLLGGQELIVGADGTISSF
                     ELGAGQVAVWTYEGEDKTPQLGDVDASVGIAGNKITISGQGFGNSKGQVTFGEISAEI
                     LSWSDTLITLKVPTVPANYYNISVTTADKQTSNSYQAFEVLTDKQIPVRLLINDFKTV
                     PGEQLYLMGDVFEMGANDAKNAVGPLFNNTQTIAKYPNWFFDTHLPINKEIAVKLVKK
                     DSIGNVLWTSPETYSIKTGHEAQTITIKK"
     gene            complement(11089..12792)
                     /locus_tag="VC122_001042"
     CDS             complement(11089..12792)
                     /locus_tag="VC122_001042"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003062376.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-glycosidase"
                     /protein_id="HEP5640974.1"
                     /translation="MNVAGMLHIPDSRYCFAISQKELVIRLRVAKEDRGITVNLVHGP
                     KYGYHEFQEELEMSVAYTDHTHAYYETTLTLDDVRLAYIFKIEEEGQTYYFSEDGLTT
                     SYDFSNGFYNFFQMPYINAVDVHQVIPWTKDAVFYQIFVDRFNRGDWEKGDSYVNMGW
                     LEKPTPKSFAGGDLKGITEKLDYLKDLGITVIYLTPIFQSISNHKYDISDYYAIDPQF
                     GTKYDLQELIDLAHQMGIKIILDAVFNHASSDAVEFQDVLRYGKESKFFDWFMTHDEH
                     PSMDLVNYETFAGCNYMPKWNTSNRDVQDYLIEIGRYWIKEFCIDGWRLDVSDEVSHD
                     FWRRFRQAVKAEKADAILIGENWHDAYPYLAGDQYDGIMNYAFTKACLDYFAFETMTS
                     KQLAEKLSNILMRNTWQVNRMNLNLLDSHDTHRFFTQVKGSKDKLLAALALLFIFMGI
                     PCLYYGTEVAMEGGYDPDSRRGFPWDDAERDKDFFSKVKAIIALRQQPALQEGAISID
                     YDEDCLILKRSLGEQSISLVLNNGESAYSIKEGGQILTSNGVTKNKKQLLQDGYIVFS
                     N"
     gene            complement(12958..14217)
                     /locus_tag="VC122_001043"
     CDS             complement(12958..14217)
                     /locus_tag="VC122_001043"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003062623.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="extracellular solute-binding protein"
                     /protein_id="HEP5640975.1"
                     /translation="MSWNWKKASVLGTLSLASVLPLTACVSGGGKGVKETDGKTIVVS
                     VDEGYVDYIKSIKGEFEKEHKVTVKVKKEGMMDTLDKLSTDGPTGASPDVFLAPFDRV
                     GGLGTEGQISEVTLGNSKEFDDTVKKLVTIDGKTYGAPDVIETLVTYYNKDLVPQAPK
                     SFTELEVLQKDSKFAFASEPGKSVGFLAKWTDFYYGYGLIAGYGGYIFGDKGTKPSDL
                     GLGNDGTVEGLNYAKQWYGTWPQGMQDTKKAGDFITEQFISKKAGVIIDGPWAASSFK
                     DAGVNFGVMEIPTLTNGKKYQPFAGGKAWVISNYSKGKTTAQKFLDYVTNAENQKRFY
                     DKTQEIPANLTARNYASKEGNELTKAVISQFESAQPMPNIPEMAEVWEPGANMFFNVA
                     SGKEEASKAAKEAAKTIKEAIEQKYAE"
     gene            complement(14826..15809)
                     /locus_tag="VC122_001044"
     CDS             complement(14826..15809)
                     /locus_tag="VC122_001044"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009880554.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HEP5640976.1"
                     /translation="MVIIAVVIFFGVILGILIVEYGQKRSFSSWLVEKMFAYRKYKLS
                     KKVDNYKGVETQRNRNIPYHLDNPEEIVGFSTQEYDVNGIQVFIINDQKQSHQPVIYY
                     LYGSVYLRRPEKLHFKTVKHLIQLTNAKVVFPNFPKVPYATYQDVYPKLVEAYQQFCL
                     SNGPSQVSVIGDSSGGGMALAFAKYIRDTKTALQPKQLILISPWLDVSFKNQAYKDFE
                     AEEAYLDARRLRDIGELWAGGQGNMQLPYVSPLYGDCEDLPEITTFVGTKEIFYPDIM
                     SLHDQLTRQRIANQLIIAEKMIHAYVIFPIREAKQARKQIAELVLKTVEPT"
     gene            complement(15840..17090)
                     /gene="dltD"
                     /locus_tag="VC122_001045"
     CDS             complement(15840..17090)
                     /gene="dltD"
                     /locus_tag="VC122_001045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002984219.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanyl-lipoteichoic acid biosynthesis protein
                     DltD"
                     /protein_id="HEP5640977.1"
                     /translation="MLKRLWLILGPLLIAFVLVVITIFSFPTQLDHSIAQEKANAVAI
                     TDSSFKNGLIKRQALSDETCRFVPFFGSSEWSRMDSMHPSVLAERYKRSYRPFLIGKR
                     GSASLSHYYGMQQITNEMQKKKAIFVVSPQWFTAQGINPSAVQMYLSNTQVIEFLLKA
                     RTDKESQFAAKRLLELNPGVSKSNLLKKVSKGKSLSRLDRAILKCQHQVALREESLFS
                     FLGKSTNYEKRILPRVKGLPKVFSYKQLNALATKRGQLATTNNRFGIKNTFYRKRIAP
                     KYNLYKNFQVNYSYLASPEYNDFQLLLSEFAKRKTDVLFVITPVNKAWADYTGLNQDK
                     YQAAVRKIKFQLKSQGFHRIADFSKDGGESYFMQDTIHLGWNGWLAFDKKVQPFLETK
                     QPVPNYKMNPYFYSKIWANRKDLQ"
     gene            complement(17083..17322)
                     /gene="dltC"
                     /locus_tag="VC122_001046"
     CDS             complement(17083..17322)
                     /gene="dltC"
                     /locus_tag="VC122_001046"
                     /EC_number="6.1.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002984216.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanine--poly(phosphoribitol) ligase subunit
                     DltC"
                     /protein_id="HEP5640978.1"
                     /translation="MSIEETVIELFDRLFMEDVSEMMDEDLFDAGVLDSLGTVELIVE
                     LESTFNIKVPISEFGRDDWNTVTKIVQGVEELQHA"
     gene            complement(17340..18596)
                     /gene="dltB"
                     /locus_tag="VC122_001047"
     CDS             complement(17340..18596)
                     /gene="dltB"
                     /locus_tag="VC122_001047"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002989574.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanyl-lipoteichoic acid biosynthesis protein
                     DltB"
                     /protein_id="HEP5640979.1"
                     /translation="MMMFFSHIPYMEPYGNPIYFVYLILAFLPVIIGIFKQKRLSTYE
                     TLVSLVFILFMFGGDHYQQLVAFLFYLLWQIISVFAYQKYREKANSAGVFYLAIAMAL
                     FPLIWVKVAPLTGPSSQTLFSFLGISYLTFKSIGMIIEMRDGTLQEVRLPDFIRFMIF
                     FPTFSSGPIDRFRRFQEDYHKLPERDDYFAMLNKAVMYLMLGFLYKHIISYCLGGILL
                     PLLENKALMVGGYFNKETILVMYVYGLNLFFDFAGYSMFAIGISYLLGIRTPENFNMP
                     FLSASLKDFWNRWHMSLSFWFRDYVFMRLVHLLIKHKTFKNRNVTSGVAYLVNMLVMG
                     FWHGLTWYYIAYGLFHGIGLIINDAWIRKKKEINRHRKKKGLSPLFQSRAFHVLCIVV
                     TFHVVMFSLLLFSGFLNDLWFNRPLH"
     gene            complement(18593..20131)
                     /gene="dltA"
                     /locus_tag="VC122_001048"
     CDS             complement(18593..20131)
                     /gene="dltA"
                     /locus_tag="VC122_001048"
                     /EC_number="6.1.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011054604.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanine--poly(phosphoribitol) ligase subunit
                     DltA"
                     /protein_id="HEP5640980.1"
                     /translation="MIKDMIDSIEQFAQTQADFPVYDCLGEHRTYGQLKRDSDSIAAF
                     IDSLALLAKSPVLVFGAQTYDMLATFVALTKSGHAYIPVDVHSAPERILAIIEIAKPS
                     LIIAIEEFPLTIEGISLVSLSEIESAKLAEMPYERTHSVKGDDNYYIIFTSGTTGQPK
                     GVQISHDNLLSFTNWMIEDAAFDVPKQPQMLAQPPYSFDLSVMYWAPTLALGGTLFAL
                     PKELVADFKQLFTTIAQLPVGIWTSTPSFADMAMLSDDFCQAKMPALTHFYFDGEELT
                     VSTARKLFERFPSAKIINAYGPTEATVALSAIEITREMVDNYTRLPIGYPKPDSPTYI
                     IDEDGKELASGEQGEIIVTGPAVSKGYLNNPEKTAEAFFTFKGQPAYHTGDIGSLTED
                     NILLYGGRLDFQIKYAGYRIELEDVSQQLNQSPMVASAVAVPRYNKEHKVQNLLAYIV
                     VKDGVKERFDRELELTKAIKASVKDHMMSYMMPSKFLYRDSLPLTPNGKIDIKTLINE
                     VNNR"
     gene            complement(20143..20286)
                     /locus_tag="VC122_001049"
     CDS             complement(20143..20286)
                     /locus_tag="VC122_001049"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003051342.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="teichoic acid D-Ala incorporation-associated
                     protein DltX"
                     /protein_id="HEP5640981.1"
                     /translation="MIKNKSRMRGIAVFIGKTILFYLILMLLVYFFGYLGHGQSNFIY
                     NEF"
BASE COUNT         6516 a         4147 c         3405 g         6281 t
ORIGIN      
        1 taattgacat atcatctggc acttttatat cataaaaaga taataactgt accatacgaa
       61 tagccagaac atcaccaaca acaaccaagg cagttgcttt aaaggaagat atagtttcca
      121 tcaatatttc tacgctaaca ggatccttac gatcaaataa cagcattggc ttcgttttta
      181 aagatcgctt aaaacaaccc ttcaaatagc caagataacg ctcagaagtt acttctgata
      241 gtaaatcatc tgttataaat aatatgttgt gatggccttt ttgataaagg tgttctactg
      301 ctgttttagc cattagctga ttatcattat caatataagt ggttttatta gtgtcacctt
      361 gtggagcacc tacaatgaca aatggtaagt tatttgtcat caaatatttt ttgacaggat
      421 catcttgatc agaataaaga ataataaaac catcaactcg tttttgaaga tgcatcagtt
      481 tcacttgctc tatcaggtca tcaagagata tacctgtagc aatcgacaca gtaaaatgag
      541 attctttagc ttcgttagta atagtcgaca aaatttccat aaagaaaggt tcacttaatc
      601 tatcgctagt tgtaatagga ggaaaaacaa gtcctatatt gtgtgtcaaa ccactcgcta
      661 gaatctgtgc tgcgacattt ggaacatatc ccaaatctgc catcgctttt ctaaccttct
      721 cttttgtttt catagaaata gatcgattat cttttaatac cctactcacc gtcgaagggt
      781 taacacctgc cttttgagcg acatctttta ttgttaccat attaacctcg ttactatatt
      841 taccaaagat aaaccttatc atgggtttgt catgtatttc gtttccatta tacaattatc
      901 tttcaaaaaa tcaacttcaa tcaagattcc cttgtcattt taactcaaat tctctaaact
      961 atttttaaaa aaaacgacct ttttttaaaa aaaggcttga ctttttctgc aagcggttgc
     1021 ataatattaa gtgtaagaaa tataccttag gaggaaatag ctatgaaatc atggcaaaaa
     1081 gttatcgtcg gcggagcaag tttgacactt gcaagtacct tgttagtggg atgtggatca
     1141 ggttcaaaag ataaaaagga agctggcgct gactcaaaga ctattaaact ttgggtaccg
     1201 actggctcta aaaaatcata tgcagatacc attgcaaaat ttgaaaaaga ttctggttac
     1261 accgttaaag tggttgaatc agaagatcca aaagcacaag aaaaaatcaa aaaagatgcc
     1321 tcaactgctg ccgatgtctt ttcacttcct catgatcaac ttggtcaact tgtagaatct
     1381 ggtactattc aagaagttcc agaaaagtac aataaagaaa ttgctgctac ttctactgat
     1441 caggcgctcg tcggtgcaca gtacaaaggg aaaacatatg ctttcccatt tggtattgaa
     1501 tcacaaattc ttttctacaa taaatcaaaa ttagctgctg aagacgttac ttcatatgat
     1561 acaattacta caaaagcaac ttttggtggt acctttaaac aagctaacac ttacgctact
     1621 ggtccattat tcatgtcagt tggtaacact ttatttgggg aaaacggtga agatgtaaaa
     1681 ggtactaact ggggcaatga aaaaggcgct gctgttctta aatggatcgc agaccaagct
     1741 tcaaataaag gttttgttag cttagatgct aacaacgtta tgtctaaatt tggtgacggt
     1801 tcagtagctt cttttgaatc aggaccgtgg gactatgaag ctgctcaaaa agctatcggt
     1861 aaagaaaacc ttggtgttgc tatttatcca aaagtaacta ttggaggcga aacggttcaa
     1921 caaaaagcat tcttgggtgt aaaactttac gcagtcaacc aagcaccagc taaaggtgat
     1981 acaaaacgta tcgcagctag ctacaaattg gcatcctact tgactaatgc tgaaagccaa
     2041 gaaaaccaat tcaagactcg taacattgtc ccagccaaca aagaagttca atcttcagaa
     2101 gctgttcaat caaacgaact tgctaaaacc gttatcacta tgggttcttc ttcagactac
     2161 actgtcgtaa tgccaaaact tagccaaatg ggcacattct ggactgaaag tgctgctatt
     2221 cttagtgatg ccttcaacgg taaaatcaaa gaaagcgact accttactaa gttgcaacaa
     2281 ttcgacaaag atatcgctgc aacaaaataa tatgatctca tgtcatcact gctatactaa
     2341 aacaagcaca aggtaagtac ttctaaaaag ttctttccac ttaccttatg aagagtggaa
     2401 ctaggctcca tcacatttta ttcaagctta gtctcactct tacttgtttt tgttttacta
     2461 gtagcttagt gctacttaaa tttttaactc tttaaaagtt tacgtaacac ttagctattt
     2521 catcctaaca agagattgaa tctgaactac cactctagta tcctaaaata aaggagaatc
     2581 tatatgactc agtcaaactt gacaccaaat atatctgtaa tagaagcttt aaaaagaggc
     2641 agttgggata ttaaactttc gagtattatc atgggatttg ccaactttgc taacaaacag
     2701 tttatcaaag gaatcttatt tttaataagt gaacttatct ttctagtagc ttttgtctcc
     2761 caaatcatcc ctgctatccg tggccttgtc actcttggta ctcaaactca gggaatgact
     2821 acaaaaacta ttgatgggat taacatccaa gttgctgttg atggcgataa ctctatgcta
     2881 atgcttattt ttggtttagc atcactcatt ttctgcctag tctttgctta catttactgg
     2941 tgtaatctca aaagtgctcg taacctttat cttttcaaac aaaaaggtca gaagatacca
     3001 aacttcaaag aagatcttgc cacacttaca aatggtcgct ttcatatgac tttaatggct
     3061 atcccactga ttggtgtgct tttattcacc attcttcctt tgatttacat gatctgtttg
     3121 gcctttacca attttgatca taatcacttg cctccaaaat ctttatttga ttgggtggga
     3181 cttgctaact ttggaaatgt tttaagcggc cgtatggcag gaaccttttt tcctatcttc
     3241 tcatggactt tgatttgggc tgtttttgcc acagtaacta atttcttctt tggtattatt
     3301 cttgctttgt taatcaatgc caaaggatta aagtggaaaa aaatgtggcg aaccatcttt
     3361 gttatcacca ttgcagttcc acaattcatc tcattattga ttatgcggaa cttactcaat
     3421 gacgaaggcc cactaaatgc tcttctcaat aaaatcggct tgattaatgg ctcactgcca
     3481 tttttatctg atcctctttg ggcaaaattc tcaatcattt ttgtcaacat gtggatcggt
     3541 attccattta ccatgttgat tgcgactggt attatcatga accttccaag tgaacaaatt
     3601 gaggctgctg aaattgatgg cgctagcaaa ttccaagtct tcaagtctat cacattccct
     3661 caaattctct tgatcatgac accaaacttg attcaacaat ttatcggaaa tatcaataac
     3721 tttaacgtca tttacctcct tactggtggt ggtccaacaa attcagaata ctatcaggca
     3781 ggaacaacag acttgctggt cacttggctc tataaattaa ccgtcacagc tgctgactac
     3841 aatttagctt ctgttatcgg tatcttaatc tttacagttt cagctatctt tagcttactt
     3901 gcttatacaa ggacagcatc ctacaaggaa ggagcggcta aataatgaaa aacaaacgtc
     3961 gatttcaact tggacttgtt tatgctactt taatcatcct ctcaatcatt tggctcttcc
     4021 caatcgcttg ggttattctt actagcttcc gtagcgaagg aactgcttac gtcaattatt
     4081 ttattcctaa gacttttaca ttaaatcact acattaatct ttttactaat gaaacctttc
     4141 cttttggaaa atggtttatg aatactctta ttgtagccac ttttacatgt attatttcaa
     4201 ccttcataac tgttgccata gcctactctc ttagccgaat taagtttaag tttcgcaatg
     4261 gctttttaaa actagccctt attttaaata tgttccctgg tttcatgagc atgattgcca
     4321 tctactatat cttaaaggcc ttaggtttga cgcaaaccct aacagcactt gtccttgttt
     4381 attcctcagg tgcagccctt ggtttttaca ttgcaaaagg attttttgat accattcctt
     4441 attcacttga tgaatcagct atgattgatg gcgctactcg tatggatatt ttctttaaaa
     4501 taacccttcc tttagcaaaa ccaatcattg tctatactgc ccttcttgct tttatggggc
     4561 cttggattga ctttatcttt gcacaagtta tcttaggtga tgctacaagt aaatataccg
     4621 ttgctattgg actattttca atgcttcagc cagataccat taacaactgg tttatggcct
     4681 tcactgctgg ttctgtctta attgccgtcc caatcactct cctcttcatg tttatgcaaa
     4741 aatactatgt tgaaggcatc acaggtggtt ctgttaaata agttacaaca taccaaacct
     4801 tctaagggca agccctttag gaggtttttg ataaataagg ccgtagataa tcatattagt
     4861 cctcacattt atcttcagat cagccaaatg tattaatgat tccttgactt ttaccatgaa
     4921 ttcggatata acaaaatgat gacatctaaa attaccatta aagatattgc acgagaagcc
     4981 ggggtttctg tggcgaccgt atcttacgtg ttaaaccagc gcaatgatca aaaaatcagc
     5041 gaagaaaccc gcaataaagt gctacatatt agtaaccttc ttaactatac acctaatcag
     5101 gcagccaggg cattagtcac acaacaatca aacagtgttg tcctgtatta caaatatgca
     5161 gatagcattt tagctagggc tgaacaactg cataccatta gctttctatc ccgcctattg
     5221 catgagcagg actatcagct ggtttgccta aatgaagata gcattgaaaa atttgactgg
     5281 gcagacctta ttctgacctt ggacgttgat aatgatacct tttacaaaat tggaaagctt
     5341 aatttcaaac ccttaattgc cattgatagc tttgttaatg atcccctctt ctttcaaatt
     5401 aatacagatt atgctgctct caaagatcag gctaatcagt tttttaagga tgaaccttat
     5461 accctgcttt ccttgcctat cactaatcgt gagcgatatg agcatgtttc tgccttcttt
     5521 ccaagtttgc acattgtaaa cgacctaaga gagctaacct actttgaaaa ggaaaatctt
     5581 ttactcattg atgagagttt ggatcaattc tttgtgaact cttcgcaaaa actctactgc
     5641 ccacgttttt ccccaaaaaa agggcaagtt atcttagagg ccatcgaatg ggccaaaaaa
     5701 cgccagcctg ataaaaaaca taaccttctg gtgtaacgcc tgagaattaa cctatcttga
     5761 ctaattcctt gtaactcaaa accttgcctc tttagataaa gaagcaaggt tttttgatgt
     5821 ctaatctaat aacagggcct gacttgtctc atcaatctct aaactgtgtt ttgtagaaca
     5881 cccaggtcag cattaataag gtaccaaata cttggcaatt cataatcagt actggatcct
     5941 gaataaagaa actaagagca atcgccagta gactcggcca agccaaacaa ttaaccatca
     6001 atccaagtcc ttcagagaag tttcgaatca taaatagccg tgatcgttta gtcagagtca
     6061 aaaagaaagc gcctagacca atcacaataa gggtacccac acacaccatg agaacaaacg
     6121 tcagcaaaag aaaaccaagt acactggctt tattactgtc ataccattgc tgattgacaa
     6181 agtccttcac tgctttaact gttgtcaggt cagcgagtga ctgctgctgg cctctaacgt
     6241 gagcacggag ttccttgcca tcaggggtaa caaaacgcca ttcttttttt gtcacaataa
     6301 tttgtctggt tgattctgat gccaattgct ctaaatcgac ctttgtcggt aaaacagcca
     6361 aaaccaattc accatctcgt cgaatcagag attgaccctg aaacgtatct ttctcaatct
     6421 ttccttgggt caatgcttga taggttttgt ctgtgatagg cgacaaactc ttagttacaa
     6481 tccgttccaa gggatacgtt gtcatgttag catagtgtaa cgtaactgga atcattatta
     6541 aggcgttcaa aaaaacaata ataagaagat tttgccacca agtaaactga gctcggctgg
     6601 caaacattgc cctagcagaa aggcaatgtg aaatgtaaga aataggaaat tgttttttat
     6661 ctggcattaa acattactcc ttgtcaggtt accctttggt accaccactt gttaaaccgg
     6721 aaacgaaatt tttctgtagg aagaaaaaga gaactgaaat aggcactgca atcagaatag
     6781 ctcccgcagc aaataaggtt accttgggat ttctagcatc attcgtaata aacgattgaa
     6841 gaccaactgc taccgtataa ttttcttgcg cccttaaaag gaatttagcc aacatgaagt
     6901 ctccgaaagg acccataaaa gcccataagg attgtactgc aatcattggt cgcacaagag
     6961 gaaggacaat ttggtagaag gtcctaaagt gtccagaacc atctaactta gcagattcgt
     7021 ccaagtcata aggtaccgtg tcaaaataac ccttcatcaa ccaggcattc ataggaatac
     7081 caccaccgac gtaaatcaaa atcaagaacc agtattggtt taaggcatta aagagccacg
     7141 ccataacaaa gtaagccgtt aaagccgcca tggtaggaac catctgtacg actaggaaaa
     7201 agattaaact ttttttgcgt ccaaagaaat tgtaacggct atacgcataa cctgtcaaag
     7261 tgatgactgt tacttgtatg agcatggtga agaatgccac gatcaaggta ttccaatacc
     7321 atcttaagta taaggtatct tgaaagagtg ttttaaagtt agacaaggtc catggcccat
     7381 caaaatgaaa actaaaggct gtggtatttc ctggtctaaa agctgaactg accgtcacta
     7441 gtattgggaa taaaatcacg atcactaagg agattagata gagataagtc aataattggg
     7501 tgaagaaacg tttgcgtttc atggatattg aaacataggt ctttctcatg atctctcctc
     7561 catatcaaaa gctttaaatt tcttaaaggt aatcaacgat accgaaatca ctattacaga
     7621 gatgattaag gtcactgccg acgccatcga aaactgaggc gaggtttgtg tggtcaactt
     7681 gtaaatccaa gaaatcagaa tatcagttgt cccagctccg ccccccacag taccagggcc
     7741 cccattatta aacagataaa taatagagaa attattgaag ttaaaggtgt actgagagat
     7801 aaaggttggt gctgcaaccg caaaaatcat tggcatggtg atatggcgga atttctgtaa
     7861 gactgtcgca ccatcaacgg ttgctgcttc gtataaatca gctggaattg cctgtaaaat
     7921 acctgatacc aaaatataga tatagggaaa tcctagccaa ccttgcacca taataacagc
     7981 cacttttgtc cagaaggggt ctgtcttcca ggacacaatt ccgaagtcaa cgaaaggaag
     8041 gaatttctcg atgaaaggaa gaacctgaac attcatggcc ccaatggaat cattaaagaa
     8101 attaccaaaa ctcataatag agataaatgc tggaacagcc catggtaaca agaatatcac
     8161 accaaagaga cgttttcctc tgataaagga ttgattagca ataacagccg ttgcaattcc
     8221 aataacaatt tgaagacttg tcgctgctaa tgtccagatc agcgtccatg acaaaacaga
     8281 tgtaaatgct gttttgaagg tgcttaattc taagatatta agaaaattct tcaaccctac
     8341 ccaatccaat aaacgatacg gaggaatatg cctaaaatcg taattggtaa aagcaatcag
     8401 caaggtcact aaaactggaa aaataatagc gaaagccatt gctaaataag ccggaatgat
     8461 gagcaagtaa ggaaatcctt tttcataaat cgcttcaagg gtctctttag ccgtcaccgg
     8521 aaccttcttg ccttcattca tttgatgtgc aactaattta gcatctctca tagcaaccaa
     8581 atgaaaaata ccaaataaga ctagagtaat taattgcatg gcccccttaa ttaacataaa
     8641 taaggagtga tcttgcattg gaacacttcc aagactgtaa aaacctgtta aagcaggaat
     8701 tcctagaact acaatttcta gtagttctag gaagaaaaca actaataaga ctcccccttt
     8761 aacgatttgt ttattgtata gttgtcccaa acctgggata acagatagcc acatggcttt
     8821 attaggcttc tgttgtcttt ggttatctac cattttttat tcctcttctc ataaatgatt
     8881 taaggttata ctccttatca tactaacttc cttttaagct gactaattta tttttttata
     8941 gtaatggttt gtgcttcatg acctgtcttt atactataag tctcaggact tgtccataaa
     9001 acattcccaa tactatcttt tttaacaagt ttgactgcta tttctttatt gattggtaga
     9061 tgagtatcaa agaaccagtt tgggtacttg gcaatggtct gagtgttatt aaatagagga
     9121 ccaacagcat tcttagcgtc atttgccccc atctcaaaaa catcacccat gagatatagt
     9181 tgttcccctg gtactgtctt aaaatcattg atgagtaaac gaacaggaat ttgtttatca
     9241 gtcaatactt caaaggcttg gtaactattg ctggtttgct tatcggcagt tgtcactgaa
     9301 atgttataat aatttgctgg aaccgtcggt acttttaagg tgataagggt atctgaccaa
     9361 gaaaggatct cagcagagat ttctccaaaa gtcacttgac ctttagaatt accaaaacct
     9421 tgacctgaaa tagtaatctt atttccagca atacccactg aagcatcgac atctccaagt
     9481 tgtggtgtct tgtcctctcc ttcataagtc catacagcga cttgccccgc tccaagttca
     9541 aagctactaa tagtgccatc tgctccaact attaattctt gaccgccaag aagaccttct
     9601 aatttgtcct tgtagctatt ttggggcaag gctgttttag cattagtaat tgtataacca
     9661 ttcgtttgat ctctattaat agccactagt gcgacatgat taccaaattt acgctcaaat
     9721 accaaaacat gatcactaat ccaacgttgt tcagttgttc cataagctaa agcttgattt
     9781 tgttttctta aaggagctag cttactaatc actttatagg cttgtgactc tttattaaaa
     9841 cttggcatat caccacgatt attaggatct ttatcacctg ttgcatactg ctctgtacca
     9901 taataaatat taggcacacc tctagatgtt aaaagcaaag cataggcttg attaacagca
     9961 gtttgattat tagccacttt tgttgcgaag cgttccatat catgattatc aataaaggtc
    10021 acctggtctg tcacttcatt agtcacctga tctctatttt caagaacctt gtaaaaatct
    10081 cgcatggtaa acgttgaaaa acctgtatac aactgtctaa tagcattggc aaatctaaaa
    10141 tctaaaagcc ccatcccact attgttagca aatgtcgtca tatcataatc atcgtcggta
    10201 tgtcccgaga accattcccc gaaaacaaag acattatgtt tttcatagat atgactcaac
    10261 caattttttt gccaaccttg tgacatatgt ttaacagcgt caactcggat accatcaaca
    10321 cccaggtcta accatttatc aatagcttct ttcatgtact ggtcaacttt cggattaatg
    10381 ttatttaaat cagctagtcc gtacattgaa tgataaatag aattttcata agtactaaaa
    10441 tcggtccaag attcatgatt aaaaatcttg tctttatcat ctgaaaattt accaactaat
    10501 ttaccgtttt tatataaagc gccatcttct ttgaaagttg tgccttcttt ttcggctgta
    10561 gacgtatgat taggagcaaa atcaataact accttaatat ggtgttgatg agctactttg
    10621 actagttgtt gaaagtctgc ttcagtgcca aaatgctggt ttgttttaaa gaagtcctta
    10681 gcccaatacc catgatatgc agcacttcca ttagaaggat caatactgtc gatattttca
    10741 acaggagaag aaatccaaat ggcagaaatc cccatatctg tcaggtaacc atccttaatc
    10801 ttggcgatga ttccttgcca atcacctcca tggtattttt taaggtcatt tttatcaaaa
    10861 acatcagtct taccattatt agaggtatta ccatcgttaa aacgatctgt tacaatctga
    10921 taaatcgtat ctgttgaaaa atctgctttg ttggtgacag aagcattatc ggcagcagaa
    10981 acagttagtg gaaatgaaat taagccaagt aaaacagcac ttttcgttaa taacttataa
    11041 gttttgatat gtaattctct catcgctttt tccttttcaa ataacctttt agttactaaa
    11101 cacaatatat ccatcctgga gtaattgttt tttgttttta gttaccccat tgcttgtcaa
    11161 aatctgtcct ccttccttaa tagaataagc actctcacca ttgttaagaa caagtgaaat
    11221 gctttgctca cctaagctgc gtttcagaat gaggcagtct tcgtcataat caatgctaat
    11281 agctccttct tgaagagctg gctgttggcg aagagcaata atcgctttta ctttgctaaa
    11341 gaaatccttg tcccgctcag catcatccca aggaaaaccg cgacgcgaat cagggtcgta
    11401 acccccttcc attgccactt ctgtcccata atacaagcaa ggaatgccca taaagataaa
    11461 gagcaatgct aaagcagcta acaatttatc tttggaaccc ttaacttgag tgaagaaacg
    11521 atgcgtatca tgagaatcca aaagattcaa gttcatgcga ttgacttgcc aagtgtttcg
    11581 catcaaaatg ttgcttaatt tctcagccaa ttgttttgat gtcatggttt caaaagcaaa
    11641 gtaatccaaa caggctttgg tgaaagcata attcattatc ccatcatact gatcccctgc
    11701 caaataagga taggcatcat gccagttctc cccaattaaa atggcatctg ctttttccgc
    11761 tttaaccgcc tgacggaaac gtcgccaaaa atcatgagat acttcatcag aaacatctag
    11821 gcgccaacca tcgatacaaa attctttaat ccagtatcgc ccaatctcaa tcagatagtc
    11881 ttgcacgtct ctatttgagg tattccattt gggcatatag ttacatccag caaaggtttc
    11941 atagtttact aggtccatgg atgggtgttc atcatgagtc ataaaccaat caaaaaactt
    12001 agactcttta ccgtatctca agacatcctg aaattccacg gcatcactgc tggcatggtt
    12061 aaagacagcg tctagaataa tcttgattcc catttggtga gctaaatcaa tcaattcttg
    12121 caaatcatat ttagttccaa attgtggatc aatagcgtag taatcactaa tatcatattt
    12181 gtgattacta atggattgaa aaattggcgt taaatagatg acagtaatcc ctaaatcttt
    12241 gaggtaatct aatttttctg taattccctt taaatctcca ccagcaaaac ttttaggagt
    12301 cggtttttct aaccaaccca tgttgacata actgtcacct ttttcccaat ccccacggtt
    12361 aaagcgatca acaaagattt gataaaaaac cgcgtctttt gtccaaggaa tgacttgatg
    12421 aacatcaaca gcattgatat aaggcatttg gaagaaatta taaaacccat ttgaaaaatc
    12481 gtaactagta gttaacccat cttcactaaa ataataggtt tgaccttctt cttcaatttt
    12541 aaaaatgtag gccaacctca catcgtctaa tgttaaagta gtttcataat aagcatgcgt
    12601 atggtctgta taggcaactg acatttccaa ttcttcttgg aattcatgat aaccatattt
    12661 aggcccgtgg accaaattaa cagtaatacc tctatcttct ttggccacgc gtaatcggat
    12721 gacaagttcc ttttgagata tggcaaagca atacctcgaa tcaggaatat ggagcattcc
    12781 ggcaacattc atttctacct ccattaataa agtattgacg attacttctt tttctttaaa
    12841 acgaaaaaat agcagagcga ctcatacttc tatattttta gtatctgttt catcttcatc
    12901 aaactaacag aaatagaagc tgggcgtact tctttatccc agcctccctt ctttgcctta
    12961 ttcagcgtat ttttgttcaa tagcttcctt aatagttttt gctgcttctt ttgcagcttt
    13021 agatgcttct tccttaccag aagccacgtt aaagaacata tttgcacctg gttcccaaac
    13081 ttcagccatt tctggaatat ttggcattgg ttgtgcgctc tcaaactgac taatcacagc
    13141 tttcgttaac tcattaccct ctttagaagc atagttacgt gctgttaagt tagctggaat
    13201 ttcttgtgtt ttatcataga aacgtttttg gttttcagca ttggtcacat aatcaaggaa
    13261 tttttgagca gtcgtttttc ctttagaata gtttgaaata acccaagctt taccacctgc
    13321 gaacggttgg tattttttac catttgtcaa agtaggaatt tccattaccc caaagttaac
    13381 accagcatct ttaaaggagc tagctgccca tggaccatca ataatgacac cagctttttt
    13441 agaaataaat tgttcagtga taaaatcacc agctttttta gtgtcttgca ttccttgagg
    13501 ccaagtgcca taccactgtt tcgcgtagtt taacccttca acagttccgt cgttacctag
    13561 gcctaaatca cttggtttgg ttcctttatc accaaagata taaccgccat aaccggcaat
    13621 taaaccataa ccataataga agtctgtcca tttcgccaag aaacctacag attttcctgg
    13681 ttcagaggca aaagcaaact tagaatcttt ttgtaagact tctaattcag taaagctttt
    13741 aggtgcttga ggcactaaat ccttattata ataagttacc aaagtttcaa ttacgtctgg
    13801 tgcaccataa gttttaccat caatggttac taatttctta accgtatcat caaactcttt
    13861 gctgtttcct aaagtaacct cagagatttg accttcagtt cctaagccac ctacacggtc
    13921 aaaaggtgct aaaaagacat ctggtgaagc tcctgttgga ccatccgttg atagcttatc
    13981 aagagtatcc atcatacctt cttttttgac tttaacggtg actttatgct ctttttcaaa
    14041 ctcaccctta atactcttaa tgtaatcaac atatccctca tcaacagaga ctacaatagt
    14101 ctttccgtct gtttccttaa cgcctttacc accaccactt acgcaagcag ttaggggtag
    14161 cactgaagct aaggataaag ttccaagcac agatgctttt ttccaattcc atgacataac
    14221 atgttcctcc cgaaatggtt ttttcttaaa cacaaatcaa atcatctatc tactctcatc
    14281 atattagcaa ataaaagata acataaggca tttaaaatta ataacagtaa tagtttcgaa
    14341 acttaaacgt ttaagttaag tttatcgtaa tcgctttcat ttgtcaataa cttttttact
    14401 tttcttttaa gagcatatca gtagtttttc tgctaattga aaacaaggca tttactgtca
    14461 cacaagtctg tcctttcttt tttctacaac tttatttcgt cagcgtaaat aaaacaaaag
    14521 ctctataatc tcttgaatgg gtaaacccac tgtagagatt atagagcttt ttgaatatca
    14581 aaaaagtccc ataaaattta taatgaaaag caaccaaact ctcattagaa agactcttat
    14641 ggaacatatc aatcatacct tacaacacat cggaattaaa gacaaaaata tcactctaaa
    14701 caatgttttg aaggatgaat ctcctactga gctcgttact acttaccaga aaggtagata
    14761 ggaaatcctg atgaagagtg tgtccaaggg attatcaatt aggtcttaga ttacaaaata
    14821 gtaattcagg taggctctac tgttttcaac actagttctg caatttgttt tcgagcttgc
    14881 ttagcttctc ttattggaaa aataacatac gcatgaatca ttttttctgc aataatcagc
    14941 tgattagcaa ttcgttgtct tgttaattga tcatgtaaag acataatgtc tgggtaaaaa
    15001 atttcttttg tcccaacaaa ggtcgttatt tcaggcaaat cctcacaatc accatacaaa
    15061 ggactgacat aaggaagttg catattacct tgaccacctg cccatagttc acctatatca
    15121 cgaagacgac gagcatctag gtaagcttct tctgcttcaa aatccttgta ggcttgattt
    15181 ttaaaactaa catctaacca aggagatata agaattaatt gctttggctg tagtgctgtt
    15241 ttagtatctc gtatatactt agcaaacgcc aaagccatcc ccccaccaga agaatcacca
    15301 atgacactaa cttgactcgg tccattagac aagcaaaatt gctggtaagc ttcaactaat
    15361 ttaggataga catcttgata agtagcatac ggtactttag gaaagttagg aaaaacaacc
    15421 ttggcattag tcagctgtat aagatgctta accgtcttaa aatgaagttt ttcgggtcgt
    15481 cgtaaataga cactgccata aagataataa ataacaggct gatgactttg cttttgatca
    15541 ttaataataa aaacttgtat cccgttcaca tcgtattctt gagttgaaaa acctactatc
    15601 tcttcaggat tatctagatg ataagggata ttacgattac gctgcgtctc aaccccctta
    15661 taattatcaa cttttttaga taacttatac tttctataag caaacatttt ctcaaccaac
    15721 caacttgaaa aggagcgctt ttgaccatat tcaactatca aaatacctag tatcactcca
    15781 aaaaaaatca ccacagcaat aatgaccact tattatcctc gttttctaat attattttac
    15841 tattgcaagt ctttcctatt tgcccaaatt ttactataaa aataagggtt cattttatag
    15901 ttgggcactg gctgcttcgt ttctagaaat ggttgcactt tcttatcaaa agctaaccag
    15961 ccattccaac cgagatggat ggtatcttgc ataaagtagg actcaccacc atcttttgag
    16021 aagtcagcaa tgcgatgaaa tccttgtgac tttaactgga attttatttt acgaactgcc
    16081 gcttgatact tatcttgatt taagccggta taatccgccc aagctttatt aacaggagtt
    16141 ataacaaaga gtacatctgt ttttcgttta gcaaattctg ataataaaag ctgaaaatca
    16201 ttgtattctg gtgacgccag gtaactataa ttaacttgga aattcttata aagattgtat
    16261 ttaggtgcta ttcgtttacg ataaaatgta tttttaatcc caaaacggtt gttggttgtt
    16321 gctaattggc ctctcttagt tgctaacgca ttcaattgtt tatacgaaaa tactttaggt
    16381 aatcccttaa cgcgaggcaa aattcttttt tcatagttag tagatttgcc taaaaaacta
    16441 aaaagggact cttctctcaa tgctacttga tgttgacatt tcaaaatagc tctgtctaac
    16501 cgactaagag acttaccctt acttactttt ttcaataagt ttgatttaga cacaccaggg
    16561 ttaagctcaa gcaaacgctt tgctgcaaac tgtgattctt tatcagttct agcttttagt
    16621 aaaaattcaa tcacttgagt gttagacaag tacatctgaa ccgcactagg attaatccct
    16681 tgagcagtaa accattgagg agatactaca aagatggctt ttttcttttg catttcattg
    16741 gtaatttgtt gcataccata ataatgcgac aaagatgctg atcctctctt accaattaaa
    16801 aatggtctat agctccgctt gtagcgctct gcaagcaccg aagggtgcat actatccatt
    16861 cgactccatt cgctagaacc aaaaaaaggc acaaaacgac aagtctcatc tgataaagct
    16921 tgtcttttaa tcaaaccatt tttaaaagaa ctatctgtga tcgcaacggc atttgctttt
    16981 tcctgagcta tggaatgatc aagttgtgta ggaaaactaa aaatagtaat cactactaaa
    17041 acaaaggcaa taagaagagg acctagaatt aaccagagtc tcttaagcat gttgcaattc
    17101 ctctacacct tgaacaatct tagttactgt gttccaatca tcacgaccaa attctgaaat
    17161 aggtacttta atattgaacg ttgactctaa ctcaacaatc aattcgactg tcccaagact
    17221 atccaacaca cctgcatcaa acaagtcttc gtccatcatc tctgaaacat cttccataaa
    17281 caaacggtca aacaattcaa ttacagtttc ttcaatagac atttttatac ttccttttat
    17341 taatgtagtg gtctgttaaa ccaaaggtca tttaaaaaac cagagaataa cagaagtgaa
    17401 aacatgacaa cgtggaaagt taccacgata cataagacat ggaatgccct gctttgaaag
    17461 aggggtgata agcctttttt cttacggtga cggttgattt cttttttctt acgaatccaa
    17521 gcatcattaa taataagtcc tataccatgg aaaagcccat aagcgatata ataccaggtt
    17581 aagccatgcc aaaatcccat gactagcata ttaaccagat aggcaacacc tgaagtgaca
    17641 tttcggtttt taaaggtttt atgcttaatc aatagatgaa ccaatcgcat aaacacataa
    17701 tctctaaacc agaaggacaa actcatgtgc catctattcc aaaaatcttt caagcttgca
    17761 gataagaaag gcatgttaaa gttttctggt gttcgaatac ccaataggta ggaaatacca
    17821 atcgcaaaca tcgaataacc agcaaagtca aaaaagagat tcaagccata cacgtacata
    17881 accagaatag tttccttatt aaagtagcca ccaaccatca aagccttatt ttcaaggaga
    17941 ggaagtaaga tgccacccaa acaataggaa atgatatgct tgtataggaa acctaacatg
    18001 agatacatga ctgccttatt gagcattgca aaataatcat cacgttcagg aagtttatga
    18061 taatcttcct gaaaacgtct gaagcgatca atggggccac ttgagaaagt ggggaagaaa
    18121 atcatgaagc gaataaaatc cggtaaacga acctcttgga gagtcccgtc acgcatctca
    18181 ataatcattc caatactttt aaaggtcaaa tatgaaatcc ctagaaaact aaagagtgtt
    18241 tgagaagaag gacctgttag gggagcaact ttgacccaga taagggggaa taaggccatc
    18301 gcaatagcta agtaaaacac tcctgcacta ttagctttct cacggtattt ctggtaagca
    18361 aaaacactga tgatttgcca caaaagataa aagagaaaag caactaattg ttgataatga
    18421 tctcctccaa acataaagag aataaatacc aaacttacca aggtttcata agtgcttaag
    18481 cgcttttgtt taaaaatccc aatgatgact ggtaagaaag ccaaaataag atagacaaag
    18541 taaatggggt taccgtaagg ttccatgtag ggaatgtggc taaaaaacat catcatcggt
    18601 tattgacctc attaatcaaa gttttaatat ctatcttacc atttggagtt aatggtagac
    18661 tgtctcgata aagaaactta gaaggcatca tataactcat catatgatcc ttaacagagg
    18721 ctttgatagc tttggttaac tccaattccc tatcaaaacg ctctttaacc ccatctttga
    18781 caacgatata ggccaagaga ttttgaacct tatgttcttt attgtacctt ggaacagcca
    18841 cagccgatgc taccattggt gattgattca actgttggga aacatcttct agttcaatac
    18901 gataaccagc atatttaatc tgaaaatcca aacgcccacc atacagcaaa atattatctt
    18961 ctgtcagtga gccaatgtcc cctgtatgat aagcaggctg tcctttaaaa gtaaagaaag
    19021 cttctgcggt tttctcagga ttgttgaggt aaccttttga cacagcaggc cctgtaacaa
    19081 taatttcacc ctgctcccct gaagctaact ccttaccatc ttcatcaata atataggtcg
    19141 gagaatctgg tttggggtag ccaatcggca agcgggtata attatccacc atttctctgg
    19201 taatttcaat agcggataag gctacagtcg cttctgttgg tccataggca ttgatgatct
    19261 tggcactcgg gaaacgttca aagagttttc gagccgtaga aacggttaat tcttcgccat
    19321 caaaataaaa atgcgttaaa gctggcatct tagcctggca aaaatcatcg ctaagcatgg
    19381 ccatatcagc aaatgaagga gtcgatgtcc aaatgccaac tggaagttgc gcaatcgttg
    19441 taaataactg tttaaagtct gccaccaatt cttttggaag tgcaaataag gttccaccta
    19501 aagccaaggt aggtgcccag tacatgacag aaaggtcaaa ggaatagggt ggctgcgcca
    19561 gcatttgtgg ctgtttaggt acatcaaatg ctgcatcttc gatcatccag ttggtaaagc
    19621 tcaaaagatt gtcatgtgaa atttgcaccc cttttggttg gcccgttgtt ccagaggtga
    19681 aaataatgta ataattatca tctcctttaa cagaatgtgt tcgctcatag ggcatttctg
    19741 ctaattttgc tgactcaatc tctgatagtg agacaaggga aatcccttca atagtaagag
    19801 ggaattcctc aatagcaatg attaaactgg gctttgcaat ctcaataatc gctaaaatac
    19861 gttctggtgc tgaatgcaca tctactggga tataggcatg accagatttg gttaaagcta
    19921 caaaagtagc taacatatca taagtttgcg ccccaaaaac caaaactgga gattttgcca
    19981 gtaaagctaa gctatctata aatgcagcaa tgctatcaga atctcttttg agttgtccgt
    20041 aagtgcggtg ttcccctaaa caatcataaa ctgggaaatc agcctgtgtc tgagcaaact
    20101 gctcaatact gtcaatcata tccttaatca tcagtcaatc tcctaaaatt cattataaat
    20161 aaagttactt tgaccatgtc ctagataacc aaagaaatag accagcaaca ttaaaatcag
    20221 ataaaataaa attgtcttgc caataaaaac agcaattcct ctcattcttg atttgttttt
    20281 tatcatactt taccttcttg tattagtgaa ataatgtcct aaaaaattct actcgaaaaa
    20341 acctaactt
//
LOCUS       DATXIG010000028        19763 bp    DNA     linear   BCT 28-DEC-2023
DEFINITION  TPA_asm: Streptococcus pyogenes strain MGAS11113
            SAMN08555799-rid19682583.denovo.028, whole genome shotgun sequence.
ACCESSION   DATXIG010000028 DATXIG010000000
VERSION     DATXIG010000028.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN08555799
            Sequence Read Archive: SRR6740495
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pyogenes
  ORGANISM  Streptococcus pyogenes
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 19763)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 19763)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-OCT-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-OCT-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001918058.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001918058.2
            Genome Coverage       :: 368x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/12/2023 11:23:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 1,806
            CDSs (total)                      :: 1,769
            Genes (coding)                    :: 1,722
            CDSs (with protein)               :: 1,722
            Genes (RNA)                       :: 37
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1, 1 (5S, 23S)
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 47
            CDSs (without protein)            :: 47
            Pseudo Genes (ambiguous residues) :: 0 of 47
            Pseudo Genes (frameshifted)       :: 30 of 47
            Pseudo Genes (incomplete)         :: 20 of 47
            Pseudo Genes (internal stop)      :: 9 of 47
            Pseudo Genes (multiple problems)  :: 11 of 47
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..19763
                     /organism="Streptococcus pyogenes"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN08555799-rid19682583.denovo.028"
                     /strain="MGAS11113"
                     /serotype="Emm28"
                     /isolation_source="normally sterile site"
                     /host="Homo sapiens"
                     /db_xref="taxon:1314"
                     /country="Finland"
                     /collection_date="2002"
                     /collected_by="National Public Health Laboratory"
     gene            11..931
                     /locus_tag="VC122_001050"
     CDS             11..931
                     /locus_tag="VC122_001050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000669594.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal ABC transporter substrate-binding protein"
                     /protein_id="HEP5640982.1"
                     /translation="MSLILGAFLSVFLLVACSSTGTKTAKSDKLKVVATNSIIADMTK
                     AIAGDKIDLHSIVPIGQDPHEYEPLPEDVEKTSNADVIFYNGINLEDGGQAWFTKLVK
                     NAQKTKNKDYFAVSDGIDVIYLEGASEKGKEDPHAWLNLENGIIYSKNIAKQLIAKDP
                     KNKETYEKNLKAYVAKLEKLDKEAKSKFDAIAENKKLIVTSEGCFKYFSKAYGVPSAY
                     IWEINTEEEGTPDQISSLIEKLKVIKPSALFVESSVDRRPMETVSKDSGIPIYSEIFT
                     DSIAKKGKPGDSYYAMMKWNLDKISEGLAK"
     gene            995..1720
                     /locus_tag="VC122_001051"
     CDS             995..1720
                     /locus_tag="VC122_001051"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004218966.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal ABC transporter ATP-binding protein"
                     /protein_id="HEP5640983.1"
                     /translation="MITTNNLCVTYDGNSNALEAINVTIEGPSIVGIIGPNGAGKSTF
                     MKAILNLIDYQGHVTVDGKDGRKLGHTVAYVEQRSMIDYNFPITVKECVALGTYSKLG
                     LFRRVGKKQFEQVDKVLKQVGLEDFGHRPIKSLSGGQFQRMLVARCLIQESDYIFLDE
                     PFVGIDSVSEKIIVDLLKELKMAGKTILIVHHDLSKVEHYFDKLMILNKHLVAYGNVC
                     EVFTVDTLSKAYGNHLILGKEMV"
     gene            1721..2575
                     /locus_tag="VC122_001052"
     CDS             1721..2575
                     /locus_tag="VC122_001052"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011184245.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal ABC transporter permease"
                     /protein_id="HEP5640984.1"
                     /translation="MSMKFFEGLMSYHFLQNALITAVVIGIVSGAVGCFIILRSMSLM
                     GDAISHAVLPGVALSFILGVNFFIGAIIFGLLASVIITYIKENSVIKGDTAIGITFSS
                     FLALGVILIGVANSSTDLFHILFGNILAVQDSDKWITIGVSIFVLVVISLFFKELLLT
                     SFDPILAKSMGVKVNAYHYLLMVLLTLVAVTAMQSVGTILIVALLITPAATAYLYANS
                     LKVMLVMSSLLGALASVLGLYLGYTFNVAAGSSIVLTSAMMFLISFFVSPKQGYLKRW
                     MQKKEKTP"
     gene            complement(2722..3528)
                     /locus_tag="VC122_001053"
     CDS             complement(2722..3528)
                     /locus_tag="VC122_001053"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002990808.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HEP5640985.1"
                     /translation="MKKLLSLSLVAISLLSLSACESVDRAIKGDKYIDEKTAKEESEA
                     ASKAYEESIQKALKADASQFPQLTKEVGKEEAKVVMRTSQGDITLKLFPKYAPLAVEN
                     FLTHAKKGYYDNLTFHRVINDFMIQSGDPKGDGTGGESIWKGKDPKKDAGNGFVNEIS
                     PFLYHIRGALAMANAGANTNGSQFYINQNKKNQSKGLSSTNYPKPIISAYEHGGNPSL
                     DGGYTVFGQVIDGMDVVDKIAATSINQNDKPKQDITITSIDIVKDYRFKN"
     gene            3745..6150
                     /locus_tag="VC122_001054"
     CDS             3745..6150
                     /locus_tag="VC122_001054"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011017450.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA translocase FtsK"
                     /protein_id="HEP5640986.1"
                     /translation="MVKRNQRRKSAPKKCLTKAEVEKQRAIKRMILSVLMALLLIFAM
                     LRLGVFGVTTYNMIRFLVGSLAYPFMFAWLIYLFCFKWLRQKDGMIAGVVIAFLGLLV
                     EWHAFLFAMPRMLDQDIFLGTARLITRDLLALRVTEFVGGGMLGALLYKPIAFLFSNI
                     GSYFIGFLFILLGLFLMTPWDIYDVSHFVKEAVDKLAVAYQENKEKRFIKREEHRLQA
                     EKEALEKQAQEEEKRLAELTVDPETGEIVEDSQSQVSYDLAEDMPKEPEILAYDSHLK
                     DDEASLFDQEDLAYAHEEIGAYDSLSALASSEDEMDMDEPVDVDFTPKTHLLYKLPTI
                     DLFAPDKPKNQSKEKNLVRKNIKVLEDTFQSFGIDVKVERAEIGPSVTKYEIKPAVGV
                     RVNRISNLADDLALALAAKDVRIEAPIPGKSLIGIEVPNSEIATVSFRELWEQSDANP
                     ENLLEVPLGKAVNGNARSFNLARMPHLLVAGSTGSGKSVAVNGIISSILMKARPDQVK
                     FLMVDPKMVELSVYNDIPHLLIPVVTNPRKASKALQKVVDEMENRYELFSKVGVRNIA
                     GYNAKVEDYNRQSEQKQMPLPLIVVIVDELADLMMVASKEVEDAIIRLGQKARAAGIH
                     MILATQRPSVDVISGLIKANVPSRIAFAVSSGTDSRTILDENGAEKLLGRGDMLFKPI
                     DENHPVRLQGSFISDDDVERIVNFIKDQAEADYDDAFDPGEVSDNDPGFSGNGGAAEG
                     DPLFEEAKALVLETQKASASMIQRRLSVGFNRATRLMDELEEAGVIGPAEGTKPRKVL
                     QTN"
     gene            complement(6220..6573)
                     /locus_tag="VC122_001055"
     CDS             complement(6220..6573)
                     /locus_tag="VC122_001055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010921962.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3397 domain-containing protein"
                     /protein_id="HEP5640987.1"
                     /translation="MMIYKLTALAFLIVTPLFAMIVTPLFKLNKKGFKFPDVALLLFA
                     IEIVLVSGKFFTHNLLPYYLIIMSLLAIVISLLLVIRTQSFSYHRFMKLFWRVGFLVT
                     VIFYLILVIFIFTLA"
     gene            6817..7242
                     /gene="rplK"
                     /locus_tag="VC122_001056"
     CDS             6817..7242
                     /gene="rplK"
                     /locus_tag="VC122_001056"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004226069.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L11"
                     /protein_id="HEP5640988.1"
                     /translation="MAKKVEKLVKLQIPAGKATPAPPVGPALGQAGINIMGFTKEFNA
                     RTADQAGMIIPVVISVYEDKSFDFITKTPPAAVLLKKAAGVEKGSGTPNTTKVATVTR
                     AQVQEIAETKMPDLNAANIEAAMRMIEGTARSMGFTVTD"
     gene            7348..8037
                     /gene="rplA"
                     /locus_tag="VC122_001057"
     CDS             7348..8037
                     /gene="rplA"
                     /locus_tag="VC122_001057"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004194879.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L1"
                     /protein_id="HEP5640989.1"
                     /translation="MAKKSKQMRAALEKVDSTKAYSVEEAVALVKETNFAKFDASVEV
                     AYNLNIDVRKADQQIRGAMVLPNGTGKTQRVLVFARGAKAEEAKAAGADFVGEDDLVA
                     KINGGWLDFDVVIATPDMMAIVGRLGRVLGPRNLMPNPKTGTVTMDVAKAVEESKGGK
                     ITYRADKAGNVQALIGKVSFDADKLVENFKAFHDVMAKAKPATAKGTYMANVSITSTQ
                     GVGIKVDPNSL"
     gene            8359..9087
                     /locus_tag="VC122_001058"
     CDS             8359..9087
                     /locus_tag="VC122_001058"
                     /EC_number="2.7.4.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002985765.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UMP kinase"
                     /protein_id="HEP5640990.1"
                     /translation="MEPKYQRILIKLSGEALAGEKGVGIDIPTVQAIAKEIAEVHVSG
                     VQIALVIGGGNLWRGEPAADAGMDRVQADYTGMLGTVMNALVMADSLQHYGVDTRVQT
                     AIPMQNVAEPYIRGRALRHLEKNRIVVFGAGIGSPYFSTDTTAALRAAEIEADAILMA
                     KNGVDGVYNADPKKDANAVKFDELTHGEVIKRGLKIMDATASTLSMDNDIDLVVFNMN
                     EAGNIQRVVFGEHIGTTVSNKVCD"
     gene            9116..9673
                     /gene="frr"
                     /locus_tag="VC122_001059"
     CDS             9116..9673
                     /gene="frr"
                     /locus_tag="VC122_001059"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006148672.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome recycling factor"
                     /protein_id="HEP5640991.1"
                     /translation="MANAIIETAKERFAQSHQSLSREYASIRAGRANASLLDRIQVDY
                     YGAPTPLNQLASITVPEARVLLISPFDKSSIKDIERALNASDLGITPANDGSVIRLVI
                     PALTEETRKELAKEVKKVGENAKIAIRNIRRDAMDDAKKQEKAKEITEDELKTLEKDI
                     QKVTDDAIKEIDRMTAEKEKELLSV"
     gene            9782..10639
                     /locus_tag="VC122_001060"
     CDS             9782..10639
                     /locus_tag="VC122_001060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002990797.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S1 RNA-binding domain-containing protein"
                     /protein_id="HEP5640992.1"
                     /translation="MNDLLATVITGLIKEENANDYFIQKEGFTFTLSKAEGERQIGDM
                     VTGFAYTDIKQKARLTTKEIRSTRTSYGWGEVTEVRRDLGVFVDTGIPNKEIVVSLDV
                     LPEMKELWPKKGDKLYIRLDVDKKDRIWGLPAEPEVFQKMASPAYNNMQNQHWPAIVY
                     RLKLTGTFVYLPENNMLGFIHPSERYAEPRLGQVLDARVIGFREVDRTLNLSLKPRSF
                     EMLENDAQMIVTYLEANGGFMTLNDKSSPEEIKATFGISKGQFKKALGGLMKAKRIKQ
                     DATGTELIG"
     gene            10712..11221
                     /gene="msrA"
                     /locus_tag="VC122_001061"
     CDS             10712..11221
                     /gene="msrA"
                     /locus_tag="VC122_001061"
                     /EC_number="1.8.4.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003054395.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide-methionine (S)-S-oxide reductase MsrA"
                     /protein_id="HEP5640993.1"
                     /translation="MERAIFAGGCFWCMVQPFEEQAGILSVRSGYTGGHLPNPSYEQV
                     CAKTTGHTEAVEIIFDPEEISYKELVELYWVQTDPTDAFGQFEDRGDNYRPVIYYTTE
                     RQKEIAEQSKANLQASGRFDQPIVTTIEPAEPFYLAEDYHQGFYKKNPRRYAQSSAIR
                     HQFLEENWS"
     gene            11218..11433
                     /locus_tag="VC122_001062"
     CDS             11218..11433
                     /locus_tag="VC122_001062"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002985757.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YozE family protein"
                     /protein_id="HEP5640994.1"
                     /translation="MRKSFYSWLMTQRNPKSNAPVAILADLVFDDTTFPKHTNDFELI
                     SRYLEDQASFSFNLGQFDEIWEDYLAH"
     gene            11589..12770
                     /locus_tag="VC122_001063"
     CDS             11589..12770
                     /locus_tag="VC122_001063"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010921965.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysM peptidoglycan-binding domain-containing
                     protein"
                     /protein_id="HEP5640995.1"
                     /translation="MIITKKSLFVTSVALSLAPLATAQAQEWTPRSVTQIKSELVLVD
                     NVFTYTVKYGDTLSTIAEAMGIDVHVLGDINHIANIDLIFPDTILTANYNQHGQATTL
                     TVQAPASSPASVSHVPSSEPLPQASATSQSTIPMAPSATPSDVPTTPLASAKPDSFVT
                     ASSELTSSTNDVSTELSSESQKQPEVPQEAVPTPKAAETTEVEPKTDISEDSTSANRP
                     VPNESASEEASSAAPAQAPAEKEETSQMLTAPAAQKAVADTTSVATSNGLSYAPNHAY
                     NPMNAGLQPQTAAFKEEVASAFGITSFSGYRPGDPGDHGKGLAIDFMVPVSSTLGDQV
                     AQYAIDHMAERGISYVIWKQRFYAPFASIYGPAYTWNPMPDRGSITENHYDHVHVSFN
                     A"
     gene            13085..14857
                     /locus_tag="VC122_001064"
     CDS             13085..14857
                     /locus_tag="VC122_001064"
                     /EC_number="4.2.1.53"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017646510.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oleate hydratase"
                     /protein_id="HEP5640996.1"
                     /translation="MYYTSGNYEAFATPRKPEGVDQKSAYIVGTGLAGLAAAVFLIRD
                     GHMAGERIHLFEELPLAGGSLDGIEKPHLGFVTRGGREMENHFECMWDMYRSIPSLEI
                     PGASYLDEFYWLDKDDPNSSNCRLIHKRGNRVDDDGQYTLGKQSKELVHLIMKTEESL
                     GDQTIEEFFSEDFFKSNFWIYWATMFAFEKWHSAVEMRRYAMRFIHHIDGLPDFTSLK
                     FNKYNQYDSMVKPIIAYLESHDVDIQFDTKVTDIQVEQTAGKKVAKTIHMTVSGEAKA
                     IELTPDDLVFVTNGSITESSTYGSHHEVAKPTKALGGSWNLWENLAAQSDDFGHPKVF
                     YQDLPAESWFVSATATIKHPAIEPYIERLTHRDLHDGKVNTGGIITITDSNWMMSFAI
                     HRQPHFKEQKDNETTVWIYGLYSNSEGNYVHKKIEECTGQEITEEWLYHLGVPVDKIK
                     DLASQDYINTVPVYMPYITSYFMPRVKGDRPKVIPDGSVNLAFIGNFAESPSRDTVFT
                     TEYSIRTAMEAVYSFLNVERGIPEVFNSAYDIRELLKAFYYLNDKKAIKDMDLPIPAL
                     IEKIGHKKIKDTFIEELLKDANLM"
     gene            15016..16068
                     /locus_tag="VC122_001065"
     CDS             15016..16068
                     /locus_tag="VC122_001065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011184255.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PhoH family protein"
                     /protein_id="HEP5640997.1"
                     /translation="MQEYSIDITLTHPDDVLALFGSNERHLKLIEAHLGVIVHARTER
                     VQVIGDDEEAVELARLTIKALLVLVGRGMVVNTSDVVTALSMAESHQIDQFMALYEEE
                     IIKDNYGKAIRVKTLGQKTYVDSVKRHDVVFGVGPAGTGKTFLAVTLAVTALKRGQVK
                     RIILTRPAVEAGESLGFLPGDLKEKVDPYLRPVYDALYHILGKEQTTRLMERDVIEIA
                     PLAYMRGRTLDDAFVILDEAQNTTIMQMKMFLTRLGFNSKMIVNGDTSQIDLPRNVKS
                     GLIDATQKLQGIKQIDFVYFSAKDVVRHPVVADIIKAYETSSEEMKDLLKPKASDVEA
                     DYQPGLTKYPVIGQEH"
     gene            16114..16689
                     /locus_tag="VC122_001066"
     CDS             16114..16689
                     /locus_tag="VC122_001066"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002985750.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="uracil-DNA glycosylase family protein"
                     /protein_id="HEP5640998.1"
                     /translation="MDDLTKAIMADEANLSYTERGIFPLYDAPQTARLIIVGQAPGIV
                     AQETKLYWNDRSGIRLRDWLGVDNDTFYHSGLFGIIPMDFYYPGKGKSGDLPPREGFA
                     AKWHPPLRALMPEVELTILVGRYAQDFYLGNKAYKTLTETVRHFEDYLPDYFPLVHPS
                     PRNQLWLAKNPWFEQDLLPILQKRVEAILTK"
     gene            16848..17345
                     /gene="ybeY"
                     /locus_tag="VC122_001067"
     CDS             16848..17345
                     /gene="ybeY"
                     /locus_tag="VC122_001067"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007895872.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rRNA maturation RNase YbeY"
                     /protein_id="HEP5640999.1"
                     /translation="MYIEMIDETGQVSQEIMEQTLDLLNFAAQKTGKEEKEMSVTFVT
                     NERSHELNLEYRDTDRPTDVISLEYKPETPILFSQEDFAADPSLAEMMAEFDAYIGEL
                     FISIDKAREQSQEYGHSFEREMGFLAVHGFLHINGYDHYTLEEEKEMFTLQEEILTAY
                     GLTRQ"
     gene            17326..17733
                     /locus_tag="VC122_001068"
     CDS             17326..17733
                     /locus_tag="VC122_001068"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002262772.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diacylglycerol kinase"
                     /protein_id="HEP5641000.1"
                     /translation="MALHDNNTTKRKWKNRTITSSLEFALTGVFTAFKEERNLRSHLL
                     SACLACVAGLFFSISAIEWLFLLLAIFLVITLEIVNSAIENVVDLASDYHFSMLAKNA
                     KDMAAGAVLMISGYAVLTGLIIFIPKIWNIFVH"
     gene            17853..18749
                     /gene="era"
                     /locus_tag="VC122_001069"
     CDS             17853..18749
                     /gene="era"
                     /locus_tag="VC122_001069"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003047080.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GTPase Era"
                     /protein_id="HEP5641001.1"
                     /translation="MFKSGFVAILGRPNVGKSTFLNHVMGQKIAIMSDKAQTTRNKIM
                     GIYTTETEQIVFIDTPGIHKPKTALGDFMVESAYSTLREVETVLFMVPADEKRGKGDD
                     MIIERLKAAKIPVILVINKIDKVHPDQLLEQIDDFRSQMDFKEVVPISALEGNNVPTL
                     IKLLTDNLEEGFQYFPEDQITDHPERFLVSEMVREKVLHLTQQEVPHSVAVVVESMKR
                     DEETDKVHIRATIMVERDSQKGIIIGKQGAMLKKIGKMARRDIELMLGDKVYLETWVK
                     VKKNWRDKKLDLADFGYNEKEY"
     gene            18769..19245
                     /locus_tag="VC122_001070"
     CDS             18769..19245
                     /locus_tag="VC122_001070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009880370.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX hydrolase"
                     /protein_id="HEP5641002.1"
                     /translation="MPQDYISYIRSKVGHDKIILNFAGGILTNDDGKVLMQLRGDKKT
                     WAIPGGTMELGESSLETCKREFLEETGIEVEAVRLLNVYTHFEEVYPNGDAVQTIVFI
                     YELTAVSDMAIDNFHNEETLKLQFFSHEEIAELESVSAKHRLMLEEYFSDSFAMGH"
     gene            complement(19551..19730)
                     /locus_tag="VC122_001071"
     CDS             complement(19551..19730)
                     /locus_tag="VC122_001071"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009880371.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEP5641003.1"
                     /translation="MRYPHAHIILIGNHINYEEIFKNHYRVFGVIDTTSHKSLKSIRD
                     QIQLYLDELYNSNNG"
BASE COUNT         6097 a         3496 c         4288 g         5882 t
ORIGIN      
        1 gggaaagaga atgagcctca ttttgggtgc ttttttatct gtttttcttt tagtagcttg
       61 ttcgtcaact ggcactaaaa ctgctaagag tgataaatta aaagtcgtgg caaccaattc
      121 aattattgcc gacatgacaa aagctattgc tggtgataaa atcgatctgc acagcattgt
      181 gccaatcggt caagaccctc atgagtacga accattacca gaagatgttg aaaaaacaag
      241 taatgctgat gtgattttct ataatggtat caatctagaa gatggtgggc aagcttggtt
      301 caccaaacta gtgaaaaatg ctcaaaaaac gaaaaacaaa gattactttg ccgtgtctga
      361 tggcattgat gtgatttact tggaaggtgc aagcgaaaaa ggaaaagaag atccacatgc
      421 ttggttaaat ctcgaaaacg gaatcattta ttcaaaaaac attgccaaac aattgattgc
      481 aaaggatcct aaaaacaaag aaacttatga aaagaaccta aaagcttatg tggctaaatt
      541 ggaaaaacta gacaaagaag ccaaatcaaa atttgatgct attgcagaaa ataaaaaatt
      601 gattgtgact agtgaaggct gcttcaagta cttttcaaaa gcttacggtg tcccatctgc
      661 ttatatctgg gaaattaaca ccgaagaaga aggaacacca gatcaaattt catcattgat
      721 tgaaaaacta aaagtcatca agccatctgc gctttttgta gagtcaagtg tcgatagacg
      781 ccctatggaa accgtttcta aagatagtgg tattcctatt tattctgaga tctttacaga
      841 ttcaattgct aaaaaaggta aacctggcga tagttattat gctatgatga aatggaacct
      901 tgacaaaatt tctgaaggtc tagcaaaata agataacaat aaagcacagc gattttagtg
      961 ttgtgctttg ttttatttat gtaaaggaga caaaatgatt acaactaaca atctttgtgt
     1021 gacctacgat ggcaacagta atgctttaga agcgattaat gtgacaattg aaggtccttc
     1081 tatcgttggt attattggtc ctaatggagc tggaaaatca acctttatga aagctatttt
     1141 aaatcttatt gattatcaag gtcacgtaac ggttgatggc aaagatggtc gtaagcttgg
     1201 gcatacagta gcttacgtag aacaacgaag catgattgac tataattttc ctattactgt
     1261 taaagaatgt gtggcgctag ggacttatag taaattaggc ctttttcgcc gagtaggaaa
     1321 aaaacaattt gagcaggttg ataaggttct caaacaagtt ggtttagaag actttggcca
     1381 tcgaccgata aaatccttat ctggtggtca gtttcagcgg atgttggttg cgcgttgctt
     1441 gattcaagaa tcagactata ttttcttaga tgaaccgttt gttggcattg actcggtcag
     1501 cgaaaaaatc attgtagatc ttttgaaaga gctcaaaatg gctggcaaaa cgattttaat
     1561 tgtacatcat gatttgagca aagtagagca ttattttgac aaactcatga ttttaaataa
     1621 acatttggtg gcttatggca acgtttgtga ggtatttact gttgatacct tgtcaaaagc
     1681 ctatggcaat catttgattt taggaaagga gatggtgtaa atgtctatga aattttttga
     1741 gggcttgatg tcataccact ttttacaaaa tgcactgata acggctgtag tcattggtat
     1801 cgtctcaggt gctgtagggt gttttattat ccttaggtca atgtctctta tgggtgatgc
     1861 catctcacac gctgttttac caggggttgc tttgtcattt attttaggag tcaatttttt
     1921 tattggagcg attatttttg ggttattagc ttctgttatt attacttata tcaaggaaaa
     1981 ctctgtcatt aaaggagata cggctattgg tatcaccttt agctcttttt tggcactggg
     2041 agttatcttg ataggggtag ccaatagttc gacggactta tttcatattt tgtttgggaa
     2101 tattttagct gtgcaagata gcgataagtg gattactatt ggtgtttcga tttttgtttt
     2161 agtggttatt agtctctttt tcaaagaact attattaaca tcatttgatc ctatcttagc
     2221 caaatcaatg ggtgtgaagg tcaatgcgta tcattatttg ttgatggtcc tattaacttt
     2281 agtggctgtt acggcgatgc aaagcgtggg tactatcttg attgttgcct tactgattac
     2341 gccagcagcg acagcttatt tatatgctaa tagcttaaaa gtaatgttag tgatgtcatc
     2401 tttactaggt gctttagcat cggttttagg gctttatttg ggctatacct ttaatgttgc
     2461 cgcagggtca agtatcgtac tgacttctgc gatgatgttt ttgatcagtt tctttgtttc
     2521 acccaagcaa ggctacctca aaagatggat gcaaaaaaaa gaaaaaacac cttaaacttt
     2581 tcgctttaaa caccttagtg gtttgcaaat agtggcaaat aacatcaaag ggtgtttttt
     2641 atatgtcatc aaaagaccat aaaaaacctt atcaaaccaa ggtatttagc ctattatctg
     2701 ataaggtttt ttaagcatga tttagttttt aaagcgataa tctttgacaa tgtcaattga
     2761 ggtaatcgta atgtcttgtt ttggtttatc attttggttg atagaagtcg cggcaatttt
     2821 atcgacaaca tccataccat caatgacttg accaaataca gtataaccgc catctaagct
     2881 tggattgccg ccatgctcat aggcagagat gataggtttt gggtagttgg tacttgataa
     2941 tcccttgctt tgatttttct tgttttggtt gatataaaat tggctaccgt tagtattagc
     3001 gccagcattt gccatggcaa gagcaccgcg aatatgatat aaaaatggag agatttcgtt
     3061 gacaaagcca ttgccagcat cttttttagg atccttgccc ttccaaatcg attcaccacc
     3121 tgtgccatct cctttggggt cacctgattg aatcataaag tcgttgatca cacgatggaa
     3181 ggtaaggtta tcatagtagc cttttttagc atgggtgagg aagttctcaa cagctaaggg
     3241 agcatatttt gggaataact taagggtgat gtccccttga cttgtcctca ttacgacctt
     3301 agcttcttct ttgccgactt ctttggttag ttgtggaaat tggctagcat cagctttgag
     3361 agctttttga atgctttctt cataggcttt agaagctgct tcgctttctt ctttggcagt
     3421 tttttcatca atgtatttat cgccttttat agcgcggtca acagattcac aagcactcaa
     3481 actaagtaaa ctaatggcta ctaaactgag agataataat tttttcatgt ctactcctaa
     3541 gaatgatttt ctttatttta tcataaaatg caacagaaaa tagtagtaaa gagttaaaaa
     3601 cgcctatttt tgtgttttat gatctaacct tggttaaaag gctgctacga ctgttattat
     3661 agcacaagat gctatagaaa aacttaaaac accttggttt tttagagcta tttcctaata
     3721 gttaaaaaat ggtataatag tgttatggtt aaaagaaatc aaagaagaaa aagtgcgcct
     3781 aagaagtgtc tgacaaaagc agaagtggaa aagcaacgtg ccattaagag gatgatttta
     3841 tctgttttaa tggctttgtt gcttattttt gcgatgcttc gcttgggcgt ctttggcgtg
     3901 acaacttata atatgattcg ttttttggtg ggtagtttag cctatccgtt tatgtttgcc
     3961 tggttaattt acttattttg ttttaaatgg ttacggcaaa aagatggaat gattgcggga
     4021 gttgttattg cctttttggg attgctagtt gagtggcacg ccttcttatt tgcgatgcct
     4081 aggatgcttg atcaagatat ttttctaggc actgctcggc taattacaag agatttacta
     4141 gccctgcgcg tgactgaatt tgttggtgga ggaatgcttg gtgcattact ttataaaccc
     4201 atagcctttt tattttcgaa tatcggctct tactttattg gttttttgtt tatcttatta
     4261 ggattatttc taatgacgcc ttgggatatc tatgatgtga gtcactttgt gaaagaggct
     4321 gttgataaac tagcagtggc ttatcaggaa aataaagaaa agcggttcat caaacgagaa
     4381 gagcaccgct tacaagctga aaaagaggct ttagagaaac aagcacagga ggaagaaaaa
     4441 cgtctagcag aattgacggt tgatcctgag acgggagaga ttgttgagga tagtcagtct
     4501 caagtcagct atgatttggc tgaggatatg ccaaaagagc cagagattct tgcttatgat
     4561 agtcacctca aagacgatga ggcaagctta tttgaccaag aagacttggc ttacgctcat
     4621 gaggaaattg gggcttatga ctctttatct gctttagcat cttctgaaga tgagatggat
     4681 atggatgaac ctgttgatgt tgatttcacg ccaaaaaccc atcttcttta taaacttcca
     4741 acgatagatt tatttgcacc agataagccc aaaaaccagt ccaaagagaa aaacttagtc
     4801 cgcaaaaata tcaaggtgct tgaagatacc ttccaaagtt ttgggattga tgtcaaggta
     4861 gagcgagcag aaatcgggcc ttccgtcacc aaatatgaaa tcaagccagc tgtcggggtc
     4921 cgagttaatc gtatttcaaa tcttgctgat gatttggcac ttgctctggc tgctaaagat
     4981 gttcgtatag aagcaccaat tcctggaaaa tccttaatag gtattgaagt tcctaactca
     5041 gaaattgcaa cggtttcttt ccgcgaactt tgggaacaat ctgatgccaa tcctgaaaac
     5101 cttttagaag taccactagg taaagctgtt aatggtaacg ctcgcagttt taacttagct
     5161 agaatgccgc atcttttggt agctggttca actggttcag gtaaatcggt tgctgttaat
     5221 ggcattattt caagtatttt gatgaaggca cgtccagacc aagtgaaatt tttgatggtc
     5281 gaccctaaga tggttgagtt gtctgtttat aatgatattc cccatttgct gattccagta
     5341 gtgaccaatc ctcgtaaagc cagcaaggcg ttgcaaaaag tggttgatga gatggaaaat
     5401 cgctatgaac tctttagtaa agtcggggtt cgaaatattg ctggttacaa tgcaaaagtt
     5461 gaggattaca ataggcaatc tgagcaaaaa caaatgcctt taccattgat tgtggttatt
     5521 gtggatgagt tagctgattt gatgatggtg gctagtaaag aagtggaaga cgctattatc
     5581 cgtctggggc aaaaggcgcg tgcggcaggt attcacatga ttcttgcaac ccaaagacca
     5641 tctgtggatg ttatttctgg actgattaag gccaatgtgc catcccgtat tgcatttgcc
     5701 gtatcttcag gaacagatag tcgaacgatt ctggatgaaa atggcgctga aaagctctta
     5761 ggacgtggag atatgctctt taagccgatt gatgaaaatc accctgttcg tctgcaaggg
     5821 tctttcatct cagatgatga tgtggagcgt attgtcaact ttatcaaaga ccaagctgaa
     5881 gcggattatg atgatgcctt tgatcctggt gaagttagcg acaatgatcc tggtttttct
     5941 ggaaatggcg gagctgctga aggtgatcct ctttttgaag aagcaaaagc cttggtctta
     6001 gaaacacaaa aagctagtgc ctcaatgatt caaaggcgtt tgtcggttgg atttaatcgt
     6061 gccacgcgct tgatggatga attggaagaa gcaggtgtta tcggtccagc agaaggaacc
     6121 aaacctcgta aggtattgca gacaaactaa aagaacctga cgtctcatga tgagatgtca
     6181 ggttcttttt ttgtgatgtc aagccagttg atgagatgcc tatgctaatg taaaaataaa
     6241 gataactaga atcaggtaga aaataacggt gactaaaaag ccaactcgcc aaaatagctt
     6301 cataaagcga tggtaagaaa acgactgagt tcggatgact agcagtagtg aaatgacgat
     6361 ggctagcagc gacatgataa taaggtaata aggcagtaaa ttatgggtga aaaattttcc
     6421 tgagacaagc acaatttcta tcgcaaaaag taaaagtgcg acatctggaa atttaaatcc
     6481 ctttttatta agtttaaata aaggggttac aatcatggca aaaagaggcg ttactatcaa
     6541 gaaagctaag gcagttaatt tatagatcat cataggtcta ttttatactc aaatccctta
     6601 cttgtaaact gatacttacc tgttgtgata gaaaatgatc tttgagaggg aaagcgccta
     6661 cttcaattta aacattacaa aaggcatcag ctattttaac aaaaagactt gatttatttt
     6721 ggcaataagc tataatggta gggtatgcaa tattgcatat ctgtgggagg taaaaatctc
     6781 aattaccgcc aaaaccacaa caggaggatt tttaaaatgg ctaaaaaagt cgaaaaactt
     6841 gtaaaacttc aaatccctgc tggtaaagct acaccagctc caccagttgg accagctctt
     6901 ggtcaagcag gtatcaacat catgggcttc actaaagaat ttaacgctcg tacagctgat
     6961 caagctggta tgatcatccc agttgttatc tcagtttatg aagacaaatc atttgatttc
     7021 atcactaaaa caccaccagc tgctgttctt ttgaaaaaag ctgcaggtgt tgaaaaagga
     7081 tcaggtacac ctaacactac taaggttgcg acagttactc gtgcacaagt acaagaaatt
     7141 gctgaaacta agatgccaga tttgaacgct gcaaacattg aagctgcaat gcgtatgatc
     7201 gaaggtactg ctcgttctat gggattcact gttactgact agtcagtcaa gaacccctat
     7261 tacccgcacg acttcatcat aagagatgag tgacgtggga gattttaaat caaatcgata
     7321 tgaccacatt acaaggagaa ttttaaaatg gctaaaaaaa gcaaacaaat gcgtgctgca
     7381 cttgaaaaag tagatagcac aaaagcgtac agtgtagaag aagctgtagc attagtaaaa
     7441 gaaacgaact tcgcaaaatt tgatgcgtct gttgaagttg cttacaactt gaacattgac
     7501 gttcgtaaag cagaccaaca aatccgtggc gcaatggtat tgccaaacgg aactggtaaa
     7561 acacaacgtg ttcttgtttt tgcacgtggt gcaaaagctg aagaagcaaa agcagctggt
     7621 gcagacttcg taggtgaaga cgaccttgtt gcaaaaatca atggtggatg gcttgacttt
     7681 gacgttgtta tcgcaacgcc agacatgatg gctatcgtag gtcgtcttgg acgtgtcctt
     7741 ggacctcgta acttgatgcc aaaccctaaa actggtacag taacgatgga tgttgctaaa
     7801 gccgttgaag agtctaaagg tggtaaaatc acttaccgtg ctgacaaagc aggtaacgta
     7861 caagctctta ttggtaaagt ttcatttgat gctgacaaat tggttgaaaa cttcaaagcc
     7921 ttccacgatg taatggctaa agctaaacct gcaacagcta aaggaactta catggcaaac
     7981 gtctcaatca catcaacaca aggtgttggt atcaaggttg atcctaactc actttaagaa
     8041 aaaaattagg gcatcgccta atcatagcaa aaagacaaat ttctgaagac aaatcttcac
     8101 tagaggtttg tcttttttat ggtttaaaat ggaatgcaaa caaaaaagtg taggcgataa
     8161 taccatgaaa ggagttgatt ctcttaatga gatgtggtgt taacacatca tgagctcaca
     8221 aaaaatgata aaggaagtat cggtggtgtt aaaaaaggga ttttaattga catttattag
     8281 cctctaaaat gtcctttccc tattcataat tgagggtttg tggtaaaata gtaaggacaa
     8341 actaaggaga acatagtagt ggaacctaaa tatcaacgta tattaattaa gttatctggt
     8401 gaagcattag caggtgaaaa aggagttggc attgacattc caactgttca ggctattgca
     8461 aaagaaattg ccgaagttca tgtgtcggga gtacagatcg ctcttgttat tggtggaggc
     8521 aatctctggc gtggagagcc tgcagcagac gctggaatgg accgtgttca ggctgattac
     8581 actggcatgc ttggaacggt gatgaatgcc ctagtcatgg ctgatagtct tcaacattat
     8641 ggtgttgata cccgtgtgca aacagctatt cctatgcaaa atgtagcaga accttatatt
     8701 cgcggacgtg ccttgcgcca tctagaaaaa aatcggattg ttgtttttgg agctggtatt
     8761 ggctcgcctt atttttcaac agacaccacc gcggcactgc gtgctgctga gattgaagca
     8821 gatgctattt taatggcaaa aaatggtgtg gatggggtct ataatgctga tcctaaaaaa
     8881 gatgctaatg cggttaaatt tgatgaatta acacatggtg aagtgatcaa acgtggtctc
     8941 aaaatcatgg atgcaacggc atcgacctta tcgatggaca acgatattga tctggtggtt
     9001 ttcaatatga atgaagctgg caatattcaa cgtgttgtct ttggagaaca tattggaact
     9061 actgtatcaa ataaagtttg tgactaaaag ttagaaaaga aagagagtaa ttatcatggc
     9121 aaatgcaatt attgaaactg caaaagaacg ttttgcacaa tcccatcagt ctttatcacg
     9181 tgaatatgcc agcattcgtg cgggtcgtgc caacgctagt cttttagacc gtatccaagt
     9241 tgattattac ggggcaccga cgccattaaa tcaattggct tcaatcactg tgccagaagc
     9301 gcgtgtctta ttgatctcgc cttttgataa atcttctatc aaagatatcg agcgcgctct
     9361 taacgcatca gatttaggca ttacacctgc taacgatggc tctgtcattc gtttggttat
     9421 tccagctttg accgaagaaa cacgtaaaga attagccaaa gaagtgaaaa aagttggtga
     9481 aaatgctaaa attgcaatcc gtaatatccg ccgtgatgct atggatgatg ctaaaaagca
     9541 agaaaaagca aaagaaatca ctgaagatga gttaaaaaca cttgagaaag atatccaaaa
     9601 agtaaccgat gatgctatca aagaaattga ccgcatgacg gctgaaaaag aaaaagaatt
     9661 gctctcagtg taacaggatt tctacgaaaa atagacgact atagacacaa aaagataggt
     9721 gaccatttca gaagcgtttg gttttgattt gtccctattt ttttaaagaa agaaaaaaga
     9781 tatgaatgat ttactagcaa cagtcattac tgggcttatt aaagaagaaa acgctaatga
     9841 ctattttatc caaaaagagg ggtttacctt taccttgtct aaagcagaag gagaacgcca
     9901 gattggtgat atggtcacag gctttgccta tacagacata aagcaaaagg cacgtctcac
     9961 aaccaaagag atccgttcaa cacggacaag ttatggctgg ggggaagtga cagaggttcg
    10021 tcgtgaccta ggcgtttttg tcgatacagg aattccaaat aaagaaattg tggtttcttt
    10081 ggacgtgttg cctgaaatga aagaactatg gcctaaaaaa ggggataaac tttacattcg
    10141 tttggatgtg gataaaaaag accgtatttg gggacttcca gcagagcctg aggtctttca
    10201 aaagatggct agccctgcat acaataatat gcaaaatcaa cattggccag caattgttta
    10261 ccgcctaaaa ttaacaggaa cgtttgttta cctgcctgaa aacaacatgt taggctttat
    10321 tcacccaagt gaacgctacg cagaaccgcg cttaggtcag gttttagatg cccgtgtgat
    10381 tgggtttaga gaggtcgatc gtaccctaaa cttgtcgttg aaaccacgtt cttttgagat
    10441 gttggagaat gacgctcaga tgattgtcac ttatcttgaa gccaatggtg gcttcatgac
    10501 cttaaatgac aaatcaagtc cagaagagat taaagcaacc tttggtattt caaaaggcca
    10561 gtttaaaaaa gccttgggtg gattgatgaa agctaaacgc atcaaacaag acgccacagg
    10621 aactgaatta ataggataaa aatgctttgc cactggcaaa gttttttgtt acaatgaaga
    10681 ccattaagtg aagaaaaagg ggatatagag aatggaaaga gccatttttg caggaggttg
    10741 tttctggtgt atggtgcaac cttttgagga gcaagcaggt attttgtcag tcagaagtgg
    10801 ctacacaggt gggcatctcc caaatcctag ctatgaacaa gtttgtgcca aaacaacagg
    10861 acatacagaa gctgttgaaa ttatttttga ccctgaagag atcagctata aggaattagt
    10921 tgaattgtat tgggtgcaga cggatccaac agacgccttt gggcaatttg aagatcgtgg
    10981 ggacaactac cgtcctgtca tttactacac aacagaaaga caaaaagaaa ttgcagagca
    11041 atcaaaagca aacttgcaag cttcaggacg ttttgaccaa cccattgtca ccactattga
    11101 accagcagag ccattttatc tcgcagagga ttatcaccaa ggattttata aaaagaatcc
    11161 caggcggtat gcacaaagca gtgctattcg tcatcagttt ttagaggaga attggtcatg
    11221 agaaaatcat tttacagctg gctgatgaca caacgtaacc ctaaatcaaa tgcacctgtg
    11281 gcgattttag cagatttggt gtttgatgac accacttttc caaagcatac caatgatttt
    11341 gaactcatta gtcggtatct agaagatcaa gctagttttt cctttaattt aggtcaattt
    11401 gatgaaatct gggaagatta tctagcacat taatacgtgg ataagaaaga gtcatatcga
    11461 ctcttttgaa gggttatcac gttattatta caaaatgaca tcactttctt tcaaaaaaat
    11521 gacaagctac tttttttctt aaaaatatgc tatgatatag cttagaaaaa aataaaaagg
    11581 aagcagagat gattattact aaaaagagtt tatttgtgac aagtgtcgct ttgtcgttag
    11641 cacctttggc gacagcgcag gcacaagagt ggacaccacg atcggttaca caaatcaagt
    11701 ctgaactcgt cctagttgat aatgttttta cttatacagt aaaatacggt gacactttaa
    11761 gcacaattgc tgaagcaatg gggattgatg tgcatgtctt aggagatatt aatcatattg
    11821 ctaatattga cttaattttt ccagacacga tcctaacagc aaactacaac caacacggtc
    11881 aggcaacgac tttgacggtt caagcgcctg cttctagtcc agctagcgtt agtcatgtac
    11941 ctagcagtga gccattaccc caagcatctg ccacctctca atcgactatt cctatggcac
    12001 catctgcgac accatctgat gtcccaacga caccattagc atctgcaaag ccagatagtt
    12061 ttgtgacagc gtcatctgag ctcacatcat caacgaatga tgtttcgact gagttgtcta
    12121 gcgaatcaca aaagcagcca gaagtaccac aagaagcagt tccaactcct aaagcagctg
    12181 aaacgactga agtcgaacct aagacagaca tctcagaaga ttcaacttca gctaataggc
    12241 ctgtacctaa cgagagtgct tcagaagaag cttcttctgc ggccccagca caagctccag
    12301 cagaaaaaga agaaacctct cagatgttaa ctgcgccagc agcacaaaaa gctgtagctg
    12361 acaccacaag tgttgcaacc tcaaacggcc tttcttacgc tccaaaccat gcctacaatc
    12421 caatgaatgc agggcttcaa ccacaaacag cagccttcaa agaagaagtg gcttctgcct
    12481 ttggtattac gtcatttagt ggttaccgtc caggagatcc aggagatcat ggtaaaggat
    12541 tagccattga ctttatggta ccggttagct ctacgcttgg tgatcaagtt gctcaatatg
    12601 ccattgacca tatggcagag cgtggtattt catacgttat ttggaaacag cgattctatg
    12661 cgccatttgc aagtatttac ggaccagcct acacatggaa ccccatgcca gatcgcggca
    12721 gtattacaga aaaccattat gatcatgttc atgtctcctt taatgcttaa ttcatgctat
    12781 caaaaaaact cacgtttagg cgtgagtttt ttgacaattc tgacagagta agccctataa
    12841 gaggctgaga tacgctttta atacctgttg tttttctcgc cctttagaca gttttaacgt
    12901 tatgtctaaa agagatggtt tttattagcg caaatatggg tttaggggta aaatatagat
    12961 aattaacctg atatgatcat gataaaggta gaagaatacc tattatttcg tgacaactga
    13021 gcaatctgcc cttacctttg gagtgatgat atcactttgc ttgactattc aaggaggtct
    13081 tgaaatgtat tatactagtg gtaattacga agcttttgcg acacctcgaa aacctgaagg
    13141 ggtagatcag aaatcggctt atattgttgg tactggttta gctggtttag cagcagctgt
    13201 tttccttatt cgcgatgggc atatggctgg ggaacgcatt catctgtttg aggaattgcc
    13261 tttagcaggt ggttctttag atggtattga aaagcctcat cttggttttg tgacccgtgg
    13321 tggtcgtgag atggaaaatc attttgagtg tatgtgggac atgtatcggt ctattccctc
    13381 actggaaatt cctggtgcgt cttatttgga tgaattttat tggttggata aggatgatcc
    13441 taactcatcc aactgtcgtt tgattcacaa gagaggaaat cgtgtggatg atgacggcca
    13501 gtatacgctc ggtaaacagt caaaagaatt agtccattta atcatgaaga cagaagaatc
    13561 tctaggagat caaaccattg aagagttctt ctcagaagat ttctttaaga gtaatttttg
    13621 gatctattgg gcaaccatgt ttgcttttga aaaatggcat tctgctgtag aaatgcggcg
    13681 ctatgcgatg aggtttatcc accatattga tggtttgcca gatttcacct ctctcaagtt
    13741 caacaaatat aaccaatatg attctatggt caaaccgatt attgcttacc tagaatcaca
    13801 cgacgttgac atccaatttg acacaaaagt cactgatatt caggtggaac aaacagctgg
    13861 taaaaaggta gcaaaaacca tccatatgac ggtgtctggg gaggctaagg cgattgagct
    13921 aacacctgat gatttggttt ttgtgaccaa tggttctatt actgaaagta gcacatacgg
    13981 tagtcatcac gaagtggcta agccaaccaa agcgttaggt ggttcttgga atttatggga
    14041 aaatctagct gctcaatcag atgattttgg tcatcctaaa gtgttttacc aggacttgcc
    14101 tgctgaaagc tggtttgtgt ctgccacagc aaccataaaa cacccagcta tcgagcctta
    14161 tattgaacgt ttgacccacc gtgatttgca cgatggcaaa gtgaacactg gcggcatcat
    14221 cactattaca gattctaact ggatgatgag ctttgccatt caccgtcaac ctcattttaa
    14281 agaacaaaaa gataatgaaa ccactgtctg gatttacggt ctttattcca atagtgaggg
    14341 caattacgtc cacaagaaaa ttgaggagtg tacaggtcaa gaaatcacag aagaatggtt
    14401 gtaccacctt ggggtacctg ttgataaaat caaggactta gcgagtcagg actatatcaa
    14461 tacagttcct gtttacatgc cttatattac gagttacttt atgccacgtg tcaaaggaga
    14521 ccgtccgaaa gttatcccag atggttcagt caacttggcc tttattggta actttgcgga
    14581 atctccatct cgagatacgg tctttacgac tgagtattct attcgtactg ccatggaagc
    14641 agtgtatagc ttcttgaatg tggaacgagg cattccagaa gtctttaatt cagcctatga
    14701 tattcgtgaa ttgctcaaag ccttttatta ccttaatgat aaaaaggcaa tcaaggatat
    14761 ggatttgcca attcctgcac tgattgagaa aatcggacat aaaaaaatca aggatacctt
    14821 tatcgaagaa ttgctcaaag atgctaatct tatgtaacat gccttagagt agttaaagct
    14881 gataagttta ggcttatcag cttttttgat gatttataga aaagtgataa aggaaaagtc
    14941 tgacgtgaac gtcacataag aatttaaaat ttgttataat gtaagcaata aagatgaaag
    15001 aaagagggac tctatttgca agagtattct attgacatta ccttaaccca tcctgatgat
    15061 gttttagctc tttttggtag caacgagcgt catttgaagc taatagaagc tcatttagga
    15121 gttattgttc acgcaagaac cgagcgagtc caagtgattg gtgatgatga agaagctgtg
    15181 gaattggctc gattgaccat caaagcccta ctggttctag tgggtcgtgg catggtggtc
    15241 aacacatcag atgtggtgac agctttatct atggcagaat ctcatcagat tgaccaattt
    15301 atggcgcttt atgaagaaga aatcattaaa gataactatg gtaaggcaat acgagtcaaa
    15361 acattaggtc aaaaaaccta tgttgatagt gtcaaacgtc atgatgtggt ttttggtgta
    15421 ggacctgcag ggacagggaa aaccttctta gctgttacgc ttgcagtcac tgctctgaaa
    15481 agaggacagg taaagcgtat tattttgacg cgtcctgcgg tagaagctgg tgaaagccta
    15541 ggttttttac caggcgattt gaaagaaaag gtggatcctt acctcagacc tgtttacgat
    15601 gccctttatc acattttagg caaggaacaa accacacgcc tgatggaacg agatgtgatt
    15661 gagattgctc cgttagctta tatgcggggt cgtactctgg atgacgcttt tgttatctta
    15721 gatgaggctc aaaacacgac catcatgcaa atgaagatgt ttttgacccg tcttggtttt
    15781 aattctaaaa tgattgtcaa tggagatacg agtcagattg acctccctcg aaatgtcaag
    15841 tctggtttga ttgatgctac tcagaaatta caaggcatta aacagattga ttttgtctat
    15901 ttctctgcta aggatgttgt ccgacatcct gttgtggctg atattatcaa ggcttatgaa
    15961 acgtcctcag aggagatgaa ggacttacta aaacctaagg ctagtgacgt tgaagctgat
    16021 taccagccag gcttgacaaa ataccctgtg attggtcaag agcactaaga tttttacgat
    16081 gatgtttaat atggcctttt taaggaggat tctatggatg atttaaccaa agctattatg
    16141 gcagatgagg ctaatctctc ctataccgaa agaggtattt ttcctttata tgatgctcct
    16201 caaacagcta ggcttattat tgttggccaa gctcctggaa tagtagctca agaaaccaaa
    16261 ctttactgga atgatcgtag tggcatccgc ttacgagact ggctaggtgt ggacaatgac
    16321 accttttacc actcaggatt gtttggcatt atccctatgg atttctatta tcctggtaag
    16381 ggaaaatccg gtgacttacc acctagagaa ggttttgcgg ctaagtggca cccgccatta
    16441 agagcattga tgccagaagt tgaattgacg attttggttg gtcgctatgc tcaggacttt
    16501 taccttggta acaaggctta caaaacctta actgaaacag tccgtcactt tgaggattac
    16561 ttgccagact attttccact tgttcatcct tctccaagaa accaattgtg gttagctaag
    16621 aacccctggt ttgagcagga tttattacct atcttgcaaa aacgtgtgga ggcaattcta
    16681 accaaatagg ccctgattta cctagcattc ccatttttta ggggcttgtg gtataatggg
    16741 taaataacaa atgctgattg aaaggataag ggaaattatc tgtcagataa taatgggtgc
    16801 ccaacatacc aaatgaggtg ggcagccctt ccctaacaat taaccttatg tatatcgaga
    16861 tgattgacga aacgggacaa gtttcgcaag agattatgga gcaaacactt gatttgctca
    16921 attttgctgc tcaaaagacg ggcaaagaag aaaaagaaat gtctgtgacc tttgtgacca
    16981 atgaaagaag tcatgaactc aacttagaat accgagatac tgatcgtcca acagatgtga
    17041 tttctctaga atataagcca gaaacgccta ttttatttag tcaagaggat tttgctgctg
    17101 atcctagttt ggcagagatg atggcagagt ttgatgctta tattggcgaa ttgtttattt
    17161 ctattgacaa ggcgcgtgag cagtcccaag aatatgggca ctcttttgag cgtgaaatgg
    17221 gatttttagc tgttcatgga ttcttacata ttaatgggta tgatcactac acccttgaag
    17281 aagaaaaaga gatgtttacc ttacaggaag agattttgac tgcttatggc cttacacgac
    17341 aataatacca ccaaacggaa atggaaaaac agaaccatta cctcaagcct ggagtttgcc
    17401 ttgacagggg ttttcacagc ttttaaggaa gaacgcaatc tcagaagtca ccttttgtct
    17461 gcctgtttag cttgtgttgc tggtttattt tttagcattt cagccatcga atggttattt
    17521 ttacttctag ccattttttt ggtcattacc ttagaaattg tcaattctgc tattgaaaat
    17581 gtagttgatt tggctagtga ctatcacttt tctatgctag ctaaaaatgc caaggatatg
    17641 gcagcaggtg ctgttttgat gatttctggc tatgctgtct tgacaggact tattattttt
    17701 attcctaaaa tttggaatat tttcgttcat taaaaagaaa ggaatgggct atcctatgtg
    17761 atgatggcat aggagaataa ggaaaggaca agacggttaa gcttagtgac aagcttgatc
    17821 tgaagcccta ccgttaaggc ccgtaattat ctatgtttaa atcaggcttt gtagccattc
    17881 ttggtcgtcc aaatgttggc aaatccacct ttttaaatca cgtcatgggg caaaaaattg
    17941 ccatcatgag tgacaaggct cagaccaccc gtaataaaat tatgggcatt tacaccacag
    18001 aaacagaaca aattgtcttt attgatacgc caggtattca taaaccaaag accgcccttg
    18061 gtgattttat ggttgaatct gcttatagta ctttacgtga agttgagacg gttcttttca
    18121 tggttcctgc cgatgaaaag cgtggtaagg gtgacgacat gattatcgag cggctaaaag
    18181 cagctaagat tcctgttatt ttagttatca acaaaattga taaggttcat ccagaccaac
    18241 ttttggaaca gattgatgac ttccgtagcc aaatggattt taaagaagtg gtcccaattt
    18301 ctgccctaga aggtaataat gtgccaaccc taatcaagtt attaactgat aatcttgaag
    18361 aaggtttcca atattttcca gaagatcaga ttactgatca tccagaacgt ttccttgtgt
    18421 cagaaatggt tcgtgagaag gtactgcatt tgacgcaaca agaggttcca cattccgtag
    18481 ctgttgttgt cgaatccatg aagcgagatg aagagactga taaagtgcat atccgtgcaa
    18541 ccattatggt ggagcgcgat agccaaaaag gcatcatcat tggaaaacaa ggagccatgc
    18601 tcaaaaaaat cggaaaaatg gcgcgccgtg atattgaact catgttaggt gacaaggtct
    18661 acttagaaac gtgggtcaaa gtcaagaaaa actggcggga taaaaagctt gatttagctg
    18721 attttggcta taatgagaaa gaatactaac atagtaaagc gagatcatat gccacaagac
    18781 tacatttctt atatccgctc caaagttggg catgataaga ttattcttaa ctttgctggt
    18841 gggattttga ccaatgatga cggcaaggtg ctgatgcagt tgcgtggtga taagaaaacc
    18901 tgggctattc ctggtggtac tatggaactg ggtgagagct ctttagagac ttgcaaacgt
    18961 gagttcttag aggaaaccgg aattgaggtt gaagctgttc gcttactgaa tgtttacact
    19021 cattttgagg aagtttatcc caacggtgat gctgttcaga ctattgtctt tatctatgaa
    19081 ctaacggctg tttctgatat ggctattgat aactttcata atgaggaaac gctcaagtta
    19141 cagtttttct ctcatgagga gatagcagag ttagagagtg tctcagccaa acatcgcctg
    19201 atgttagagg aatattttag tgatagcttt gctatggggc attaagaact ctctaggaca
    19261 gttggagtat ttttggagat tggcttgtta aaaaaatttt tgaaagacga tgtttcgaga
    19321 gcttatgctc tctttttttg cttccaattt ttctgattaa gtctgaataa ttttgttaaa
    19381 tgttaacagg attttctttc caattgatac tagcagacat caaacatttg ttttagagtt
    19441 atgacgaatt gccacctagc catgcatgac ttaggcaagt tgaacctaac tgtggcaata
    19501 gacttggaag gggtgacaag aatcatttcc taaaagaatt gatgaagtgc ctatccatta
    19561 ttcgaattgt aaagttcgtc taagtaaagt tgaatttgat ctcgaatgga ctttaatgat
    19621 ttatgacttg ttgtgtcaat aacaccaaaa actcgataat gatttttaaa tatttcctcg
    19681 taattaatat gatttccgat tagaataata tgagcatgag gataacgcat acgtatttcg
    19741 tttagaaggt catatacttg ctc
//
LOCUS       DATXIG010000029        19266 bp    DNA     linear   BCT 28-DEC-2023
DEFINITION  TPA_asm: Streptococcus pyogenes strain MGAS11113
            SAMN08555799-rid19682583.denovo.029, whole genome shotgun sequence.
ACCESSION   DATXIG010000029 DATXIG010000000
VERSION     DATXIG010000029.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN08555799
            Sequence Read Archive: SRR6740495
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pyogenes
  ORGANISM  Streptococcus pyogenes
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 19266)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 19266)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-OCT-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-OCT-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001918058.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001918058.2
            Genome Coverage       :: 368x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/12/2023 11:23:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 1,806
            CDSs (total)                      :: 1,769
            Genes (coding)                    :: 1,722
            CDSs (with protein)               :: 1,722
            Genes (RNA)                       :: 37
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1, 1 (5S, 23S)
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 47
            CDSs (without protein)            :: 47
            Pseudo Genes (ambiguous residues) :: 0 of 47
            Pseudo Genes (frameshifted)       :: 30 of 47
            Pseudo Genes (incomplete)         :: 20 of 47
            Pseudo Genes (internal stop)      :: 9 of 47
            Pseudo Genes (multiple problems)  :: 11 of 47
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..19266
                     /organism="Streptococcus pyogenes"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN08555799-rid19682583.denovo.029"
                     /strain="MGAS11113"
                     /serotype="Emm28"
                     /isolation_source="normally sterile site"
                     /host="Homo sapiens"
                     /db_xref="taxon:1314"
                     /country="Finland"
                     /collection_date="2002"
                     /collected_by="National Public Health Laboratory"
     gene            118..336
                     /locus_tag="VC122_001072"
     CDS             118..336
                     /locus_tag="VC122_001072"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002984785.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HEP5641004.1"
                     /translation="MYPRIRNLREDNDFTQKFVANLLSFSHANYAKIERGEVALMADV
                     LVQFSKLYNVSIDYLLGQTDYPYRFHNK"
     gene            complement(407..550)
                     /locus_tag="VC122_001073"
     CDS             complement(407..550)
                     /locus_tag="VC122_001073"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEP5641005.1"
                     /translation="MGISPTGLLRWQPERSEVSLSLFVVMADREPLYFIANNYRSYHG
                     VSH"
     gene            637..857
                     /locus_tag="VC122_001074"
                     /pseudo
     CDS             637..857
                     /locus_tag="VC122_001074"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013903600.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3173 family protein"
     gene            1559..1942
                     /locus_tag="VC122_001075"
     CDS             1559..1942
                     /locus_tag="VC122_001075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011285518.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HEP5641006.1"
                     /translation="MKVHTTLEKDENGNWYRKDQTKTPAGERLIELDDVTIVVLENWR
                     RNQVVNTDTDFIISRFGEPFCKSTICRVIKHKAQEVGVPVITGKGLRHSHASYLINVL
                     KKDILYVAKCMGHADKSTTLNTYSH"
     gene            2205..3440
                     /locus_tag="VC122_001076"
     CDS             2205..3440
                     /locus_tag="VC122_001076"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010922278.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanyl-D-alanine carboxypeptidase"
                     /protein_id="HEP5641007.1"
                     /translation="MIKRWVILFFMVLTCSGFGKTVLAADFQVGAEHAIVVEADSGRV
                     LYEKDAKTPDAIASLTKLVTAYLVLDKVKSGQLQLSDQVNLSDYAFELTKDRSLSNVP
                     FDKKTYSVQDLLTATLVASSNSAAIALAEKVAGSELHFVNQMREQLSHWGITSGKILN
                     ASGLPNEVLKDNRYPGSALEEENTLSAQDVAVVTMHLLEDFPEILEITKQTEVNFAGN
                     SIKSFNQLLPGMAKGRAGVDGLKTGTTDLAGHCLVVTSIENGMRVITVILNADGSDKN
                     QNTRFEQANRLLDYVDRTYCRRKILKKGSLVLERSLPIQDGQVKELPVSVAEDVTIIL
                     QQGEQVPKPKQFMISETSLLAPITKGEVVAYLTSPRITDQSVRYLKEPKRIPLKASQS
                     LKKASDLQLWWRDFLEKRR"
     gene            complement(3556..4518)
                     /locus_tag="VC122_001077"
     CDS             complement(3556..4518)
                     /locus_tag="VC122_001077"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014407556.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide deacetylase family protein"
                     /protein_id="HEP5641008.1"
                     /translation="MNNRHKRRQKRKAFTLINLILLFACILGLTLIFYLYQGSNKFGL
                     SDVSQFVPKISLFHHKKTAKKETTKLKKTHFDSSKSQKKAHSKLTWTKQETPVKIPIL
                     MYHAIHVMFPEETANANLIVNPDLFDQQLQKMKDEGYYFLSPEEVYRALSNNELPAKK
                     VVWLTFDDSMIDFYNVAYPILKKYDAKATNNVITGLTEIGSAANLTLKQMKEMKQFGM
                     SFQDHTVNHPDLEQASPDVQTTEMKDSKDYLDKQLNQNTIAIAYPSGRYNDTTLQIAA
                     RLNYKLGVTTNEGIASAANGLLSLNRIRILPNMSPENLLQTMEP"
     gene            4851..6128
                     /locus_tag="VC122_001078"
     CDS             4851..6128
                     /locus_tag="VC122_001078"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002995327.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional folylpolyglutamate
                     synthase/dihydrofolate synthase"
                     /protein_id="HEP5641009.1"
                     /translation="MTYEETLEWIHDHLVFGIKPGLKRMLWVLGQLGNPQKNVKGVHI
                     VGTNGKGSTVNHLQHIFTTAGYEVGTFTSPYIMDFKERISINGRMISEKDLVIAANRI
                     RPLTERLVQETDFGEVTEFEVITLIMFLYFGDMHPVDIAIIEAGLGGLYDSTNVFQAM
                     VVVCPSIGLDHQAILGETYADIAAQKAGVLEGGETLVFAVENPSAREVFLTKAEQVGA
                     SIWEWQEQFQMAENASGYRFTSPLGVISDIHIAMPGHHQVSNAALAIMTCLTLKDRYP
                     RLTSDHIREGLANSLWLGRTELLAPNLMIDGAHNNESVAALVAVLKNNYNDKKLHILF
                     GAIDTKPIADMLVALEQIGDLQVTGFHYPNAYPLEKYPERFGRVADFKDFLALRKHAK
                     ADDFFVITGSLYFISEIRQYWKKHIEKNVLLTH"
     gene            6163..6747
                     /gene="folE"
                     /locus_tag="VC122_001079"
     CDS             6163..6747
                     /gene="folE"
                     /locus_tag="VC122_001079"
                     /EC_number="3.5.4.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002995330.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GTP cyclohydrolase I FolE"
                     /protein_id="HEP5641010.1"
                     /translation="MKRERLMSINKEKAEAAIYQFLEAIGENPNREGLLDTPKRVAKM
                     YAEMFLGLGKDPKEEFTAVFKEQHEDVVIVKDISFYSICEHHLVPFYGKAHIAYLPSD
                     GRVTGLSKLARAVEVASKRPQLQERLTSQIADALVEALNPKGTLVMVEAEHMCMTMRG
                     IKKPGSKTITTTARGLYKESRAERQEVISLMTKD"
     gene            6756..7556
                     /gene="folP"
                     /locus_tag="VC122_001080"
     CDS             6756..7556
                     /gene="folP"
                     /locus_tag="VC122_001080"
                     /EC_number="2.5.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014407558.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydropteroate synthase"
                     /protein_id="HEP5641011.1"
                     /translation="MKIGKFVIEGNAAIMGILNVTPDSFSDGGSYTTVQKALDHVEQM
                     IADGAKIIDVGGESTRPGCQFVSATDEIDRVVPVIKAIKENYDILISIDTYKTETARA
                     ALEAGADILNDVWAGLYDGQMFALAAEYDAPIILMHNQDEEVYQEVTQDVCDFLGNRA
                     QAALDAGVPKNNIWVDPGFGFAKSVQQNTELLKGLDRVCHLGYPVLFGISRKRVVDAL
                     LGGNTKAKERDGATAALSAYALGKGCQIVRVHDVKANQDIVAVLSQLM"
     gene            7563..7922
                     /gene="folB"
                     /locus_tag="VC122_001081"
     CDS             7563..7922
                     /gene="folB"
                     /locus_tag="VC122_001081"
                     /EC_number="4.1.2.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003058150.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroneopterin aldolase"
                     /protein_id="HEP5641012.1"
                     /translation="MDKIVLEGCRFYGYHGAFKEEQTLGQIFLVDLELSVDLQAASLS
                     DQLTDTVHYGMVFDSVRQLVEGEKFILIERLAGAICEQLFNEFPPIEAIKVAIKKENP
                     PIAGHYKAVGIELERQR"
     gene            7919..8419
                     /gene="folK"
                     /locus_tag="VC122_001082"
     CDS             7919..8419
                     /gene="folK"
                     /locus_tag="VC122_001082"
                     /EC_number="2.7.6.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002984757.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-amino-4-hydroxy-6-
                     hydroxymethyldihydropteridine diphosphokinase"
                     /protein_id="HEP5641013.1"
                     /translation="MTIVYLSLGTNMGDRAAYLQKALEALADLPQTRLLAQSSIYETT
                     AWGKTGQADFLNMACQLDTQLTAADFLKETQAIEQSLGRVRHEKWGSRTIDIDILLFG
                     EEFYDTKELKVPHPYMTERAFVLIPLLELQPDLKLPPNHKFLRDYLAALDQSDITLFS
                     AQQTEF"
     gene            8569..9456
                     /gene="murB"
                     /locus_tag="VC122_001083"
     CDS             8569..9456
                     /gene="murB"
                     /locus_tag="VC122_001083"
                     /EC_number="1.3.1.98"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012677902.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramate dehydrogenase"
                     /protein_id="HEP5641014.1"
                     /translation="MITELHGIDIRENEPLKHYTYTKVGGPADFLAFPRNHYELSRIV
                     AYANKENMPWLVLGNASNLIVRDGGIRGFVIMFDKLNAVHLNGYTLEAEAGANLIETT
                     KIAKFHSLTGFEFACGIPGSIGGAVFMNAGAYGGEISHIFLSAKVLTSSGEIKTISAR
                     DMAFGYRHSAIQETGDIVISAKFALKPGNYDTISQEMNRLNHLRQLKQPLEFPSCGSV
                     FKRPPGHFAGQLIMEANLKGHRIGGVEVSEKHAGFMINVVDGTAKDYEDLIAYVIETV
                     ENHSGVRLEPEVRIIGENL"
     gene            9502..10656
                     /locus_tag="VC122_001084"
     CDS             9502..10656
                     /locus_tag="VC122_001084"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011184488.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEP5641015.1"
                     /translation="MTKPIITFNNVSKTFEDSGTQVLKNINFDLEEGKFYTLLGASGS
                     GKSTILNIMAGLLDASSGDIYLDGERINDLPINKRDIHTVFQNYALFPHMTVFENVAF
                     ALKLKKVDKKEIAKRVKETLKMVQLEGYENRSIQKLSGGQRQRVAIARAIINQPRVVL
                     LDEPLSALDLKLRTEMQYELRELQQRLGITFVFVTHDQEEALAMSDWVFVMNEGEIVQ
                     SGTPVDIYDEPINHFVANFIGESNIINGTMIEDYLVSFNGKEFESVDGGMRPNEPVEV
                     VIRPEDLQITLPEEGKLQVKVDTQLFRGVHYEIIAYDELGNEWMIHSTRKAIEGEVIG
                     LDFTPEDLHIMRLNETEEEFDARIEEYVEMDEPEDGLINAIEEERNEENL"
     gene            10640..11434
                     /locus_tag="VC122_001085"
     CDS             10640..11434
                     /locus_tag="VC122_001085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012677903.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEP5641016.1"
                     /translation="MKKTSSLFSIPYFLWILFFVVAPVTLLFYKSFFDIEGRVTLANY
                     ETFFSSWTYLRMSVNSILYAGIITLVTLLISYPTALFLTRLKHKQLWLMLIILPTWVN
                     LLLKAYAFMGIFGQQGGINSFLTFMGIGPQQILFTDFSFIFVASYIELPFMMLPIFNA
                     LDDIDHNVINASRDLGASEFQAFSKVIFPLSLNGVRAGVQSVFIPSLSLFMLTRLIGG
                     NRVITLGTAIEQHFLTTQNWGMGSTIGVVLILTMVAIMWLTKEKSK"
     gene            11431..12207
                     /locus_tag="VC122_001086"
     CDS             11431..12207
                     /locus_tag="VC122_001086"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015016945.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEP5641017.1"
                     /translation="MKKFANLYLASVFVLLYIPIFYLIFYSFNKGGDMNGFTGFTLEH
                     YQTMFEDSRLMTILLQTFVLAFSSALLATIIGTFGAIFIHHVRGKYQNAMLSANNVLM
                     VSPDVMIGASFLILFTSLKFQLGMSSVLLSHIAFSIPIVVLMVLPRLKEMNQDMVNAA
                     YDLGANYFQMLKEVMLPYLTPGIIAGYFMAFTYSLDDFAVTFFLTGNGFTTLSVEIYS
                     RARQGISLDINALSTIVFFFSILLVIGYYYMSQDKEEKHA"
     gene            12200..13273
                     /locus_tag="VC122_001087"
     CDS             12200..13273
                     /locus_tag="VC122_001087"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012766865.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HEP5641018.1"
                     /translation="MRKLYSFLAGVLGVIVILTSLSFILQKKSGSGSQSDKLVIYNWG
                     DYIDPALLKKFTKETGIEVQYETFDSNEAMYTKIKQGGTTYDIAVPSDYTIDKMIKEN
                     LLNKLDKSKLVGMDNIGKEFLGKSFDPQNDYSLPYFWGTVGIVYNDQLVDKAPMHWED
                     LWRPEYKNSIMLIDGAREMLGVGLTTFGYSVNSKNLEQLQAAERKLQQLTPNVKAIVA
                     DEMKGYMIQGDAAIGITFSGEASEMLDSNEHLHYIVPSEGSNLWFDNLVLPKTMKHEK
                     EAYAFLNFINRPENAAQNAAYIGYATPNKKAKALLPDEIKNDPAFYPTDDIIKKLEVY
                     DNLGSRWLGIYNDLYLQFKMYRK"
     gene            complement(13328..13993)
                     /locus_tag="VC122_001088"
     CDS             complement(13328..13993)
                     /locus_tag="VC122_001088"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009880423.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HEP5641019.1"
                     /translation="MNVLIIEDDPMVDFIHRNYLEKLNLFDRIISSDSMKAVQSILTD
                     YAIDLILLDIHITDGNGIQFLEKWRAQHIPCEVIIISAANDGNIIRDGFHLGIIDYLI
                     KPFTFERFQESIQQFVTHREHLANQQLEQAQIDQLKCLTSKKDTKNKQLLEKGLSEST
                     FQWIIENIKAFDQPFTIQELASACHLSHVSVRKYIAYLEENKQLNSQQIFTKVGRPYR
                     VYW"
     gene            complement(13974..15515)
                     /locus_tag="VC122_001089"
     CDS             complement(13974..15515)
                     /locus_tag="VC122_001089"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014635455.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sensor histidine kinase"
                     /protein_id="HEP5641020.1"
                     /translation="MKKPLRLWASLSLILVSMIVVTTSLFYGIMLHDTHQSIKNQETH
                     LLTSTGKMLASHQAIKELLLNNQPNAKTTAYTNSIASIYNLDYVVVMNMKGIRLTRPN
                     PKNIGKPFQGGDEEAVLAGKKVISTAKGTLGKSLRYLVPVFDGDKQIGAIAVGIKLTT
                     LNDVALTSKRNYTLSLFLCLLISLLVTSFISFRLKRQLHQLEPSEIYQLFEERNAMLD
                     QIEAAVFVVDKTGILQLCNQAGQELIARKCQLGKPTGNSFNHLFPDFPKLSLQEGHEQ
                     LFRYGEEDYLLAISPICVKNDHRGHIIFMREAVKAIDTLDQLAYTTAYASALQAQTHK
                     FMNQLHVIYGLVDIAYYDQLKIYLDSILEPENEILTSLSVLVKEPLLASFLIGEQEKY
                     QELNVHLKIDVLSEIPHSVTKNQLNNGLMIYRFIHTNLLTTLRPKSLVLSIQHDQNHL
                     ISHYTLTDNWIDLERVQPIFDLPYFQQLLTDTNSQFHQHQTVTELQFSVTTPYVGWQH
                     ERFNH"
     gene            15676..17007
                     /locus_tag="VC122_001090"
     CDS             15676..17007
                     /locus_tag="VC122_001090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010922288.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-hydroxycarboxylate transporter family protein"
                     /protein_id="HEP5641021.1"
                     /translation="METISKKMPQKDLSEHSKAWQNRRIGSVPLPVYLVLATLILITG
                     WLQQLPVNMLGGFAVILTLGWLLGTIGATIPGLKHFGGPAILSLLVPSILVFFNLLNP
                     NVLEATNVLMKQANFLYFYIACLVCGSILGMNRKILIQGLFRMIIPMLLGMVCAMGVG
                     TLVGVILGLDWQHTLFYVVTPVLAGGIGEGILPLSLGYSAITGVGSEQLVAQLIPATI
                     IGNFFAILCTALLNRFGEKHPSYSGQGQLVKIGHSEDMSDALKDNSGALDVKLMGAGV
                     LTACSLFIAGGLLQHLTGFPGPVLMIILAAFLKYLNVIPQETQNGAKQLYKFISSNFT
                     FPLMAGLGLLYIPLKEVVATLSWQYFIVVISVVLTVVSVGFFVSRFLNMNPVEAAIIS
                     ACQSGMGGTGDVAILSTADRMNLMPFAQVATRLGGAITVITMTAILRIIFS"
     gene            17039..18205
                     /locus_tag="VC122_001091"
     CDS             17039..18205
                     /locus_tag="VC122_001091"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014635456.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADP-dependent malic enzyme"
                     /protein_id="HEP5641022.1"
                     /translation="MKNQLGQLALEQAKTFGGKLEVQPKVDITNKHDLSIAYTPGVAG
                     VSSAIAKDKTLAYDLTTKKNTVAVISDGTAVLGLGDIGPEAAMPVMEGKAALFKAFAG
                     VDAIPIVLDTKDTEEIISIVKALAPTFGGINLEDISAPRCFEIEQRLIKECHIPVFHD
                     DQHGTAIVVLAAIFNSLKLLQKSLDEVSIVVNGGGSAGLSITRKLLAAGATKVTVVDK
                     FGIINEQEADQLAPHHLDIAKVTNREFKSGTLEDALEGADIFIGVSAPGVLKAEWISK
                     MAARPVIFAMANPIPEIYPDEALEAGAYIVGTGRSDFPNQINNVLAFPGIFRGALDAR
                     AKTITVEMQIAAAKGIASLVPGDALSTTNIIPDAFKEGVAEIVAKSVRSVVLKS"
     gene            complement(18287..19225)
                     /locus_tag="VC122_001092"
     CDS             complement(18287..19225)
                     /locus_tag="VC122_001092"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015632209.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Zn-dependent alcohol dehydrogenase"
                     /protein_id="HEP5641023.1"
                     /translation="MKAATYLSTGNLQLIDKPKPVIIKPTYRPVGKNHSICGTDLHIL
                     GGDVPACKEGTILGPEGIGIVKEIGDAVTNFKIGDKVIISCVTSCHTCYYCKRGLSSH
                     CEDGGWILGHLINGTQAEYVHIPHADGSLYHAPDTIDDEALVMLSDILPTSYEIGVLP
                     SHVKPGDNVCIVGAGPVGLAALLTVQLFSPANIIMVDLSQNRLEAAKTFGATHTICSG
                     SSEEVKAIIDDITNGRGVDISMECVGYPATFDICQKIISVGGHIANVGVHGKPVDFNL
                     DELWIKNITLNTGLVNANTTEMLLNVLKVIIDNDIS"
BASE COUNT         5812 a         3310 c         4115 g         6029 t
ORIGIN      
        1 aaaatgacta taaaatttca atggtttact agcttatatt taatagactt gtccaaatag
       61 gacaattaaa cattttttaa tcaaatctcc tttatactga tattcggaga atgttagatg
      121 tatccgcgta ttaggaattt gagagaagat aacgatttca ctcaaaagtt tgttgcaaat
      181 ttactttcct tctctcatgc aaattatgca aaaattgaga gaggagaggt tgccttaatg
      241 gcagatgttc ttgtgcagtt ttctaaactc tataacgtca gtattgatta tttattaggg
      301 caaacagatt atccgtatcg ttttcataat aaataaaagt ctcgccttga tttgttcatt
      361 gacaactgaa taaaccgagg tgggactttc ttgtttgtcg agcaatttag tgagatacgc
      421 cgtgatagga gcgataatta ttagctataa aatagagtgg ttctctatct gccatgacca
      481 caaataggga caatgatact tctgaacgtt caggctgcca gcgtaaaagg ccagtgggtg
      541 agattcccat gagtgattta accagtcata cccacggagg ttgtaatttt tttgaaagga
      601 gtggaggtgt gaaaatagta acatataaaa agcttgatga atattggttt tctcgaacat
      661 actgcaagaa atattattag agaagcaaaa agaatagcta taaaaaagtt gaagaagctc
      721 gcgaacttga acaaaatgcg gtacaattaa gtaaatcgcc ctttgacaat agaagacttg
      781 gtattgctcc taaagatatt gtagaagagt tagtaggtgt tctactctct gaagaaagtt
      841 tagaaagtga gaattaaaga tgactataaa aaatcaaaaa aagtacaaag gtgtttattg
      901 tgataagaat gggaagatat tttatcaagc tgatcttggt gtagatccag taacaggtaa
      961 acgagttcaa aagaaagcca gaaagaatta gtatggaaag ccatttggta caatgaaaga
     1021 agcgtatgat gaactggttc ggattaggta tgagttcagt aataatctta gtattgaaaa
     1081 tgacaatata tcgtttgaaa actatatgaa tactatttac ctaagagcat ataagcaaaa
     1141 ggttcaggct gtaacttatg aaatagcctt accacatcat aagttattta ttcagtattt
     1201 tggttctaaa cttttaaagg gaataacagc ttgagattgt gaatctttta gtttacatat
     1261 tattgaaaat tatgcaaaga atttatggtc gcgattcaaa gcttgcatgg ggtatgctga
     1321 aaggttagac tatatttcaa aagtgccatg taaggctttg gataatccta aagggaaaca
     1381 tcccgacact ccattttgga cttatattga gtttcagaat tttataaaat cgtttgattt
     1441 gcaagattat gaagagctac aaagatttac aactatttgg ctatattata tgacaggtgt
     1501 tcgtgtaagt gaaggtttat cattatgatg ggaaaatatc gatttcgaac aaaaattctt
     1561 gaaagttcat acaaccttgg aaaaggatga aaatggaaat tggtatcgta aagaccaaac
     1621 aaaaacgcca gcaggagaac gtcttattga gttagatgat gtgacaatag tagtattgga
     1681 aaattggaga agaaatcaag tagtcaatac tgacacagat ttcattattt cacggtttgg
     1741 tgaaccattt tgtaaatcaa cgatttgtcg agttattaag cacaaagctc aagaagtagg
     1801 agtaccagtg ataacgggaa aaggcttgcg acatagtcat gcttcctatc ttattaatgt
     1861 tttgaaaaaa gacattctgt acgttgcaaa gtgtatggga catgctgata agtctacaac
     1921 tttgaataca tatagtcact gatttaacgc attagataaa acagtatctg aagaaataac
     1981 ccaaaatgta ataagttcag gattagattc cattttatac caaaattcct gccaaacaac
     2041 taattgatat aacgaagctt attatatcgc ccatataaca ggatgtgtta ttgttacagg
     2101 gtacttgctt tatgagcttg tttaagctac taaaggtggg tgccactata cttccttcca
     2161 aatttctgag aatttttgct ataatagtaa ctatgaaaga tataatgata aaaaggtggg
     2221 ttatcctttt ttttatggta ttaacttgtt cagggtttgg taaaacagtt ctcgcagctg
     2281 attttcaagt gggagcagaa catgcgattg tcgtagaggc agactctggg agagttctgt
     2341 atgagaaaga cgctaagacg ccagatgcta ttgcctcttt gaccaaattg gtgacggctt
     2401 atctggtttt agataaggtt aaatcaggcc agctgcaatt atcagatcaa gttaatctct
     2461 cagattatgc ctttgagtta acaaaagata ggtctttaag taatgtacct tttgacaaaa
     2521 aaacttattc agttcaagac ctgttgacag ctactttggt agcaagctca aatagcgctg
     2581 ctattgcgtt agccgaaaaa gtggcgggct cagagcttca ttttgtaaat caaatgagag
     2641 aacagctatc tcattggggg attacttctg gtaaaatcct caacgcctca ggtctgccaa
     2701 atgaggtgtt aaaggacaat cgttatcctg gttctgcgtt ggaagaagaa aataccttga
     2761 gtgctcaaga tgttgcagtt gtgacgatgc atttgttaga agattttcct gagattttag
     2821 agatcaccaa acaaacagaa gttaattttg ctggcaattc tattaaaagt tttaatcaac
     2881 tcttaccagg tatggctaag ggtagagcag gagtggatgg tttaaagact gggacaacag
     2941 acttggcagg tcattgtctt gtggtaactt ctattgaaaa tggtatgcga gtgattaccg
     3001 ttattctaaa tgccgatgga tctgataaaa accaaaacac gcgatttgag caagcaaatc
     3061 gtttgttgga ctatgtggat agaacttatt gtcgtcgtaa gattcttaaa aaaggaagtc
     3121 ttgtcttgga gcgatcgctc cctattcaag atggccaagt aaaagaacta ccggtatctg
     3181 ttgccgaaga tgtaaccatc attttgcaac agggtgaaca agtccccaaa cctaaacaat
     3241 ttatgatatc agagacttct ttactagcac cgattactaa aggtgaagtg gttgcttatt
     3301 tgacgtctcc acgaataaca gatcagtcag tgaggtattt aaaggaacct aagcgtattc
     3361 ctcttaaagc tagccaatct cttaaaaaag cctctgactt acagctgtgg tggcgagatt
     3421 ttcttgaaaa aagacgttaa agataagaat aaaataattt taaaacaatc aaaaaagaca
     3481 gttatgaaag aattttttca taactgtctt tttagtttac agtgagtatg atttggaatg
     3541 ataaaaaaat tgatgttatg gttccattgt ttgtaaaaga ttttccggac tcatgtttgg
     3601 gaggatacga atccggttaa gagaaagaag tccattagcc gcgctagcta ttccttcgtt
     3661 agtggtaaca cctaatttat agttaagtct agctgcaatt tgtaaggtag tgtcattata
     3721 tctaccagat ggataagcaa tggcaatagt gttttggttt aattgcttat caagatagtc
     3781 ctttgaatct ttcatttcag ttgtttgaac atcaggacta gcctgctcta aatcaggatg
     3841 attcactgta tgatcttgga aagacatacc gaattgtttc atttctttca tctgcttaag
     3901 agtcagattg gcagcgcttc ctatttcggt tagtcctgta ataacattat tggtagcttt
     3961 agcatcatat ttttttaaga ttgggtaagc aacgttgtag aaatcaatca tactgtcatc
     4021 aaatgttagc catacaactt ttttagcagg caattcatta tttgaaagag cgcggtagac
     4081 ctcttcagga ctcaaaaaat agtagccctc gtctttcatt ttttgaagtt gttgatcaaa
     4141 gagatctggg ttgacaatca aattagcatt ggccgtctct tcagggaaca ttacatgaat
     4201 agcatgatac attaaaatgg ggatcttgac aggtgtttct tgcttggtcc aagttaattt
     4261 agaatgggcc tttttttgag attttgagga atcgaaatga gtcttcttaa gttttgtcgt
     4321 ttcttttttt gccgtctttt tgtgatgaaa aagacttatt ttaggtacaa attgagagac
     4381 atctgacaag ccgaatttgt tagacccttg gtataaatag aaaatgaggg tcaaaccaag
     4441 aatgcaggca aataatagta taaggttaat gagagtgaag gcttttcttt tttggcgccg
     4501 tttatgtcta ttgttcatta cttgctcctt tttttgatat aagtacctct attgtaacat
     4561 tttttagaga aaagaaggct tgaagtcagc cttttttctt gttttttgga agaacgtgta
     4621 ttaaggagta aaaaaattgt tgtcacagat aacactttag gaggtagcaa agactatgtg
     4681 gggctggtat tcttatcacg taaaaaaagc aatgaagata aaagtgtagt gtactttaga
     4741 agttatttga gtgtttggtg aggataataa aaaaaaaggg tactactcta ggttatctgc
     4801 tgtcactttt ttttatatat ggtatactaa agatagatgt gaggtatgaa atgacttatg
     4861 aagagactct agaatggatt catgatcact tagttttcgg catcaagcca ggcttaaaac
     4921 gtatgctttg ggttcttgga cagttaggaa atccccaaaa aaatgtcaag ggagtgcata
     4981 tcgttggaac taacggaaaa ggttccacgg tcaatcattt acaacacatc tttacgacag
     5041 ctggttatga agtggggacc tttacctctc cttatattat ggatttcaaa gaacgtatta
     5101 gcataaatgg tcgaatgatt tcagaaaaag atttggttat tgctgctaat cgtattcgtc
     5161 ctttgacaga gcgtctagtt caagagactg attttggaga ggtaactgaa tttgaagtga
     5221 ttactttgat catgttcctc tattttggag acatgcatcc tgtagatatt gctattattg
     5281 aagcaggcct tggcggtctt tatgattcga ccaatgtttt tcaagcaatg gttgtggtct
     5341 gtccctcaat tggtttagat caccaagcta ttttgggaga gacttacgct gatattgccg
     5401 cccaaaaagc tggtgtttta gaagggggcg agacccttgt ttttgccgtt gaaaacccgt
     5461 cagctcgaga ggttttttta acaaaagctg agcaggttgg tgcttcaatc tgggaatggc
     5521 aagagcagtt tcaaatggct gagaacgcat ctggctatcg ttttactagt cctttaggag
     5581 ttatttcaga tattcacatc gctatgccag gacaccacca agtgtctaat gcagctttag
     5641 ctattatgac ttgcttaacc ttgaaagacc gctatcctag gctaacttca gatcatataa
     5701 gggaaggtct ggcaaatagt ttgtggctag gccgtaccga attattagca ccaaatctaa
     5761 tgattgatgg ggctcataac aatgaaagcg ttgctgcttt ggtagccgtc ttaaaaaata
     5821 actacaatga taaaaagctt catattttgt ttggtgcgat cgacaccaag cctattgcgg
     5881 atatgttggt tgctcttgag caaataggtg acttgcaggt tactggcttt cattacccta
     5941 acgcctatcc attggaaaaa tacccagaac gttttggtag ggttgctgat ttcaaagatt
     6001 tcttggccct gcgtaagcat gccaaagcag atgacttttt cgtgattaca gggtcgctat
     6061 attttatttc agaaattaga cagtattgga aaaaacatat tgaaaaaaac gttcttttaa
     6121 cccattaaaa aagagaaaat gaaaaggaaa aggaaaaaac taatgaaaag ggaaagacta
     6181 atgtctatta ataaggaaaa agcagaagca gccatttatc agtttttaga ggctattggt
     6241 gaaaatccaa atcgggaagg tcttcttgac acgcctaaac gggttgctaa aatgtatgcg
     6301 gagatgtttt tgggactggg aaaagatcct aaagaggagt ttacggctgt ctttaaagaa
     6361 caacatgagg atgtggtgat tgtcaaagac attagttttt attctatatg tgaacaccac
     6421 ttagttcctt tttatggcaa ggctcatatc gcttacttgc caagtgatgg tcgagtaaca
     6481 ggtttgagta aattagcgcg agcagttgaa gtagctagta aacgacctca actccaagag
     6541 cgtttgacct ctcaaatagc ggatgcttta gttgaagccc tcaatcctaa agggacttta
     6601 gttatggtag aagcagagca catgtgcatg accatgcgag gcatcaaaaa gccaggtagt
     6661 aaaaccatta ccaccactgc cagaggctta tataaggaaa gtcgtgctga gaggcaagaa
     6721 gttatttctc taatgacaaa agattaggag aacttatgaa aattggaaag tttgtgattg
     6781 agggcaatgc ggctatcatg gggattttaa atgtgactcc agattctttt tcagatgggg
     6841 ggtcttacac tactgtgcaa aaagcattag atcacgttga gcaaatgatt gctgatgggg
     6901 ctaaaatcat cgacgttggt ggagaatcaa cacgtccagg ttgccaattt gtaagcgcta
     6961 ccgatgaaat tgaccgggtg gttcctgtga tcaaggccat caaagaaaac tatgatattc
     7021 taatcagcat tgatacctat aaaaccgaaa cagctagagc agctttagag gcgggtgccg
     7081 atattctcaa tgatgtttgg gcaggtttgt acgacggtca aatgtttgcc ttagcagccg
     7141 agtacgatgc gcctatcatc ttgatgcata accaagacga agaagtttat caagaggtaa
     7201 cacaagacgt ttgtgatttt ctaggcaata gagcacaagc agctcttgat gctggcgtgc
     7261 caaaaaacaa tatttgggtt gatccaggat ttggatttgc caaatctgtt caacagaata
     7321 cggagttatt aaaaggattg gaccgcgtct gtcatttggg ctatcctgtc ttgtttggta
     7381 tttcgagaaa gcgtgtcgta gatgccttgt taggcggaaa caccaaagct aaagagcgag
     7441 acggagcgac agcagccttg tctgcttatg cccttggaaa aggctgtcag attgtacgcg
     7501 tacacgatgt caaggctaat caagacattg tggctgtgtt gagccagttg atgtgaggac
     7561 ttatggataa aatcgtatta gaaggttgcc gtttttatgg ctaccatgga gcctttaaag
     7621 aagaacagac ccttgggcaa atttttctgg ttgatttaga attgtcagtc gatttgcaag
     7681 cagcttcttt gtcagaccaa ttaacagata cggtccatta cgggatggtg tttgatagtg
     7741 tccgtcagtt agtggaaggg gaaaagttta ttttgattga acgtttagca ggtgcgattt
     7801 gtgaacagct ttttaacgaa tttccgccta ttgaagccat caaagtggcc attaaaaagg
     7861 aaaacccacc gattgcaggc cactataagg cagttggtat tgaattggag agacagagat
     7921 gaccattgtt tatttaagtt taggcaccaa tatgggggac cgagcagctt acttgcaaaa
     7981 agctcttgag gctttagctg acttacctca aacacggttg cttgctcaat catccattta
     8041 tgaaacgact gcttggggca agacaggcca ggctgatttt ctcaatatgg cctgtcaatt
     8101 agatacacaa ttaaccgcgg ctgatttctt aaaagaaaca caagctattg agcaatctct
     8161 tggtcgtgtg aggcatgaaa aatggggatc aagaactatt gacattgata tcttgctatt
     8221 tggagaagag ttttatgaca caaaggaatt gaaagtgcca cacccttata tgactgagcg
     8281 tgcttttgtt ttaatcccct tattggaatt gcagccagat ttaaaattgc ctcctaatca
     8341 taagttttta agggattatc ttgcagcttt agatcaatca gatatcacgc tcttttcagc
     8401 tcaacagaca gagttctaga ctctgttttc ggagtaatgc ctgctataag ccaatccata
     8461 aagttttgcc aaattcatcg ttattttttc taaaatttga tataatagta tcggctttat
     8521 gccgattttt ttacgcgtat aagaagtgat aaaagaaaga agataactat gataactgaa
     8581 cttcatggga ttgatattcg agagaatgaa cccctaaaac attacactta cactaaggta
     8641 ggtggcccag cagatttctt ggcttttcct agaaatcact acgagctatc acgcatcgtt
     8701 gcttatgcca acaaagaaaa tatgccttgg cttgttttgg gaaatgccag caatctcatt
     8761 gtgcgagatg gtggtattcg tggctttgtc atcatgtttg ataagctaaa tgcagtgcac
     8821 ttaaatggtt ataccttaga agccgaagct ggtgccaatc tgattgaaac aacgaagatt
     8881 gccaaattcc atagtttaac agggtttgaa tttgcatgtg gcattcctgg aagcattggg
     8941 ggtgctgttt ttatgaatgc gggtgcctat ggtggggaaa tatcacatat tttcttatcc
     9001 gctaaggtgc ttacctcaag tggagagatc aaaaccattt cagctaggga tatggctttt
     9061 ggttaccgtc actctgccat tcaagaaaca ggtgacattg tcatttctgc taagtttgct
     9121 cttaaaccgg gtaattatga tacgatcagc caagaaatga atcggttaaa tcaccttcgc
     9181 caactaaaac aacctttaga atttccatct tgtggatctg tgtttaagcg cccgccagga
     9241 cactttgcag gtcaattaat catggaagca aatcttaaag ggcatcggat tggtggtgtg
     9301 gaagtttccg aaaaacacgc tggttttatg attaatgtgg tagatggcac agctaaagat
     9361 tatgaagatt tgattgctta tgtcattgag acagttgaaa accattctgg tgtcaggctt
     9421 gaaccagaag ttcggattat tggggaaaac ctgtaaccat tattgataat taaagaaagt
     9481 accgtggagg atttatgaca attgactaag ccaattatca catttaacaa tgtttcaaaa
     9541 acatttgagg acagtggaac acaggttcta aagaacatta actttgacct tgaagaagga
     9601 aaattttaca ccctgcttgg agcttctggc tctggaaaat caactatttt aaatattatg
     9661 gcgggcctac tagatgccag cagcggagac atttatttag atggggaacg tattaatgat
     9721 ttgccgatta ataaacgtga cattcatacc gttttccaaa attatgccct cttccctcat
     9781 atgactgttt ttgagaatgt tgcctttgct ttgaagctga agaaagtgga caagaaagag
     9841 attgctaagc gcgtgaaaga aaccttgaaa atggttcaat tagaagggta tgaaaatcgt
     9901 tctatccaga agttatctgg tggtcaacgt caacgtgttg ccattgcgcg tgcgattatc
     9961 aatcaaccgc gtgtggtctt acttgacgaa ccactctcag cccttgattt gaaattaaga
    10021 acagagatgc aatatgaatt acgtgaattg caacaacgtc taggcattac ctttgttttt
    10081 gttactcacg atcaagaaga agccttggcc atgagtgatt gggtttttgt catgaatgaa
    10141 ggtgaaattg ttcagtcggg aacaccagtg gatatttatg atgagccaat taatcatttt
    10201 gttgctaatt ttattggaga atctaatatt attaacggta ccatgattga agactatctt
    10261 gtctccttta acgggaaaga atttgaatct gtggacggtg ggatgcgccc taatgagcct
    10321 gttgaagtgg ttattcgtcc tgaagatctt caaattactt tgccagaaga agggaaatta
    10381 caagttaagg ttgataccca attattccgc ggggttcact acgaaattat tgcctatgat
    10441 gaattgggta atgaatggat gattcattct acccgcaaag ctatcgaggg agaagttatc
    10501 ggattagact ttacccctga agatcttcat atcatgcgtc ttaatgagac tgaagaggaa
    10561 tttgatgccc gtattgaaga atatgtggaa atggatgagc ctgaagatgg attgattaat
    10621 gccattgagg aggagcgtaa tgaagaaaac ctctagtctt ttttcgattc cttacttctt
    10681 atggattctc ttctttgttg tggcaccagt cactctcttg ttttacaagt ccttttttga
    10741 catagaaggg cgcgtgacct tagccaatta tgaaaccttt tttagctctt ggacctattt
    10801 gagaatgagt gtgaattcta ttttatacgc tggtattatc acactcgtca cgctcttgat
    10861 ttcatatcct acggctctct ttttaacgcg cctaaagcac aagcagttgt ggcttatgct
    10921 cattattttg ccaacttggg taaacttatt gctaaaagcc tatgccttta tgggaatctt
    10981 tggtcaacaa ggaggaatta acagcttttt aacctttatg gggattggcc cgcagcaaat
    11041 ccttttcacg gatttctcct tcatttttgt agcctcttac attgagctcc cttttatgat
    11101 gttaccgatt tttaacgctt tggatgatat tgaccataat gtcattaatg ccagtcgcga
    11161 cctaggagct agtgaatttc aggccttctc aaaagttatt tttccccttt ctttaaatgg
    11221 ggttagggca ggtgttcagt ctgtctttat cccaagtttg agtctcttta tgttaacccg
    11281 tttgattggt ggaaaccgcg tgattacact tggtacagcc attgaacaac attttttgac
    11341 cacccaaaac tggggaatgg gatcaaccat aggtgtggtg ttaatcttaa ccatggttgc
    11401 tattatgtgg ctcacaaagg agaaaagtaa atgaaaaaat ttgccaatct ttatttagcg
    11461 agtgtctttg ttttactcta cattcctatt ttttatttga ttttctattc tttcaacaaa
    11521 ggtggggata tgaatggttt tacaggattt acccttgagc attaccaaac catgtttgag
    11581 gatagtcgtc tcatgacaat cttactgcaa acctttgttc ttgcttttag tagcgctcta
    11641 ctagcaacga ttattgggac ctttggagct atctttatcc accatgttag aggtaagtac
    11701 caaaatgcca tgctatcagc caataatgtc ttgatggtat caccagatgt catgattggg
    11761 gcttcctttt taattctttt tacatcattg aagtttcagc tgggcatgtc ttcagtttta
    11821 ttaagtcata ttgctttttc gattcctatt gtggttttga tggtattgcc gcgcttgaaa
    11881 gagatgaatc aagacatggt caacgccgct tatgatttgg gagctaatta tttccaaatg
    11941 ctcaaagaag tcatgctgcc atacttaaca ccagggatta ttgcaggtta ttttatggcc
    12001 tttacctatt ccttagatga ttttgcagtg actttctttt tgactggaaa tggttttact
    12061 actttatctg ttgagattta ttcgcgggct cgtcagggaa tttccttgga tatcaatgct
    12121 ttgtcaacca tcgttttctt tttctccatc ctcttagtga tcggttatta ttatatgtca
    12181 caggacaagg aggaaaaaca tgcgtaaact ttattccttt ctagcaggag ttttgggtgt
    12241 tattgttatt ttaacaagcc tttctttcat cttgcagaaa aaatcgggtt ctggtagtca
    12301 atcggataaa ttagttattt ataactgggg agattacatt gatccagctt tgctcaaaaa
    12361 attcaccaaa gaaacgggca ttgaagtgca gtatgaaact ttcgattcta atgaagccat
    12421 gtacactaaa atcaagcagg gcggaaccac ttacgacatt gctgttccta gtgattacac
    12481 cattgataaa atgatcaaag aaaacctact caataagctt gataagtcaa aattagttgg
    12541 catggataat atcggtaaag aatttttagg gaaaagcttt gacccacaaa acgactattc
    12601 tttgccttat ttctggggaa ccgttgggat tgtttataat gatcaattag ttgataaggc
    12661 acctatgcac tgggaagatc tgtggcgtcc agaatataaa aatagtatta tgctgattga
    12721 tggagcgcgt gaaatgctag gggttggttt aacaactttt ggttatagtg tgaattctaa
    12781 aaatctagag cagttgcagg cagccgagag aaaactgcag cagttgacgc cgaatgttaa
    12841 agctattgta gcagatgaga tgaaaggcta catgattcaa ggtgacgctg ctattggaat
    12901 taccttttct ggtgaagcca gtgagatgtt agatagtaac gaacaccttc actacatcgt
    12961 gccttcagaa gggtctaacc tttggtttga taatttggta ctaccaaaaa ccatgaaaca
    13021 cgaaaaagaa gcgtatgctt ttttgaactt tatcaatcgt cctgaaaatg ctgcgcaaaa
    13081 tgctgcatat attggttatg cgacaccaaa taaaaaagct aaggccttac ttccagatga
    13141 gataaaaaat gatcctgctt tttatccaac agatgacatt atcaaaaaat tggaagttta
    13201 tgacaattta gggtcaagat ggttggggat ttataatgat ttatacctcc aatttaaaat
    13261 gtatcgcaaa taagcactca aaaaggctgg tatttctcca gcctttttaa tgtatcgaca
    13321 gatgacgtca ccagtacacg cgatagggac gaccaacctt ggtaaaaatt tgttggctgt
    13381 tcagttgctt attttcttct aaataagcaa tgtatttacg gacagagaca tgggataagt
    13441 gacaagcgga agctaattct tggatagtaa aaggttgatc aaaggctttg atattttcta
    13501 taatccactg gaaagtggat tcagataagc ctttttctaa caattgtttg tttttagtgt
    13561 cttttttgct agtcaaacat ttcagttggt caatttgggc ctgctctagt tgctgattgg
    13621 ccaaatgttc tcgatgcgta acaaactgtt ggatgctttc ttgaaagcgt tcaaaggtaa
    13681 agggcttgat gaggtagtcg ataattccta aatgaaaacc atctcggata atattcccat
    13741 catttgcggc tgaaataata atgacttcgc aaggaatatg ctgagcacgc catttctcca
    13801 aaaattgaat cccattgcca tctgtaatat gaatatccag taaaatgagg tcaattgcgt
    13861 aatcagtaag tatgctttga acagctttca tagagtcgct ggatataatg cgatcaaaaa
    13921 gatttagttt ttcgaggtaa ttgcgatgga taaaatccac cataggatca tcttcaatga
    13981 ttaaaacgtt catgttgcca tcctacataa ggtgttgtca ctgagaactg taactcggtt
    14041 acggtttgat gttgatggaa ttgggaattt gtatcggtca agagctgttg aaaataaggt
    14101 aagtcaaaaa taggctgtac ccgctccaaa tcaatccaat tgtctgtcaa ggtgtaatgg
    14161 ctgattaaat gattctgatc atgttgaata ctcaaaacga gtgatttggg tctcagtgtg
    14221 gttagaagat tagtatggat aaagcggtaa atcatcaaac cattattaag ttgattcttg
    14281 gtaacagaat gaggaatctc gcttaaaaca tcaatcttga gatggacatt taattcttgg
    14341 tatttctctt gctctccaat gagaaaacta gctagaagag gctccttaac aaggacagat
    14401 aagctagtga gaatttcatt ttcgggctcc aaaatactgt ctaaataaat tttcaattgg
    14461 tcataataag caatatctac taagccataa ataacatgca attgattcat gaatttatga
    14521 gtttgggctt gtagagcaga agcataggcg gtggtatagg ctagctgatc caaggtatca
    14581 atagccttaa ctgcttctcg catgaaaata atgtggcctc ggtgatcgtt tttgacacag
    14641 atgggtgaaa tggctaacaa gtagtcttcc tcaccatacc gaaagagttg ttcatgcccc
    14701 tcttgcaaac taagttttgg aaaatcagga aagaggtgat tgaaactgtt gccagtaggt
    14761 tttcccaact ggcattttcg agcgattaac tcttgtccag cctgattgca taattgtaaa
    14821 ataccagttt tatctaccac aaatacggcg gcttcgattt gatccaacat ggcattacgc
    14881 tcttcaaaga gctggtaaat ttcacttggt tctaactggt gcagttgacg cttgagtcga
    14941 aaagaaataa aactggtgac cagtaaacta ataagaagac aaaggaataa agacagggta
    15001 taattgcgtt ttgacgttaa agccacatca tttagggtag ttagtttaat gccaaccgca
    15061 atagcaccaa tctgtttgtc accgtcaaac acaggtacta gatagcgaag tgactttcct
    15121 aaagtgccct tggcggtaga gataaccttt tttcctgcta gaacagcctc ctcatcacct
    15181 ccttgaaaag gttttccgat atttttcggg ttaggacggg tgagtcgaat ccctttcatg
    15241 ttcatcacga ccacataatc aaggttatag atggaggcaa tggagttggt ataggctgtt
    15301 gttttagcat ttggttggtt gtttaggagt aattctttaa ttgcttgatg acttgctaac
    15361 atttttccag ttgacgttag caaatgggtt tcctgatttt tgatggattg gtgcgtatca
    15421 tgtagcataa tcccgtaaaa tagggaggtt gtgacaacaa tcatagatac caaaatcaac
    15481 gataaactgg cccataaacg tagtggtttt ttcataaggt ctcctcttta ttgagtataa
    15541 cacgataaga aatgacctgc tagcttaaat taattaaata acttgaaaaa attaaagtag
    15601 ttaaacaact taaataaact atgcttttga catgttctct tttatactag tcttatcact
    15661 aaaggaggtt gtttcatgga aacaataagc aagaaaatgc ctcaaaaaga cttgtctgag
    15721 cattcaaaag catggcaaaa taggcgaata ggctccgttc ccttgcctgt ttatctggtt
    15781 ttagctacct taattttgat cacaggctgg ctccaacaat tgcctgttaa tatgctgggt
    15841 ggctttgctg ttattttaac ccttggttgg ctactaggaa ccattggagc gaccattcca
    15901 ggcctcaaac attttggtgg cccagctatt ttatcactct tagtcccttc gattttggtt
    15961 ttttttaatc tcctcaaccc caatgtctta gaagcgacga atgtcttgat gaagcaagcc
    16021 aattttcttt atttttatat tgcctgtctg gtctgcggta gtattttagg catgaatcga
    16081 aaaatcttga ttcaaggtct ttttcggatg attattccca tgttacttgg aatggtttgt
    16141 gcaatgggtg tagggacttt ggttggcgtc attttaggat tagactggca acataccctc
    16201 ttttatgttg tgacgccagt ccttgctgga ggaattggtg aaggtatttt acccttatct
    16261 cttggttaca gtgccattac aggagtcgga agtgagcaat tagtggctca actgattcca
    16321 gccaccatta ttgggaattt ctttgctatc ttgtgcacgg ctttgctcaa tcgttttgga
    16381 gagaagcacc caagttattc tggtcaaggg caactggtta aaattggtca cagcgaagac
    16441 atgtctgatg ccctaaaaga caattctgga gccctagatg ttaaattaat gggggcaggc
    16501 gttttaacag cttgttcact ttttattgct ggtggcttgt tacagcattt aacaggtttt
    16561 ccaggaccgg tgttgatgat tattctggca gccttcttga aatacctgaa tgttattccc
    16621 caagaaaccc aaaatggtgc caagcaactt tataaattta tttccagcaa ctttactttt
    16681 ccgttaatgg cagggcttgg actgctttat atccccttaa aagaggttgt ggcaaccctt
    16741 agctggcaat attttattgt ggtgattagt gtggtgttaa cggttgtcag tgtcggtttc
    16801 tttgtctctc gcttccttaa catgaaccct gttgaagcag ctattatttc agcttgtcaa
    16861 agtggtatgg gtggaacagg ggacgtggct attttgagta cggctgaccg catgaatcta
    16921 atgccgtttg cccaagtcgc tactcgtcta ggtggggcta ttaccgttat caccatgaca
    16981 gctattttaa gaattatttt tagttaatcc aaaccaataa aaaagagagg tatttcctat
    17041 gaaaaatcaa ttaggtcaac ttgcccttga acaagctaaa acgttcggag gtaaattgga
    17101 agttcaacca aaagttgaca ttacaaataa acatgatttg agcattgcct atacaccagg
    17161 tgtagctggt gtgtcatctg ccatcgcaaa agataaaaca ttagcatatg acttaaccac
    17221 caagaaaaat acagtagccg ttatcagtga tggtactgcg gttcttggtc tgggggatat
    17281 tggccctgaa gcagccatgc cagttatgga aggaaaagca gcccttttta aagcatttgc
    17341 aggtgtcgat gctattccga ttgtattaga taccaaagat actgaagaaa ttattagtat
    17401 tgtcaaggcg cttgcaccaa cctttggtgg tattaattta gaggatatta gtgcgccccg
    17461 ctgttttgaa attgagcaac gcttaattaa ggaatgtcat attcctgttt tccatgacga
    17521 tcagcatgga acagctattg tggtgttagc agccatcttt aatagtttga aacttcttca
    17581 aaaatccctt gatgaggtat ccatcgtggt gaacggtggt ggatctgcgg gattatccat
    17641 tacccgtaaa ttattagcag caggtgctac gaaagtgaca gtagtggata aatttggcat
    17701 tatcaatgaa caagaggcag atcagttagc accacaccat ttggatatag cgaaagtcac
    17761 taatcgtgag tttaaatcag gtaccttaga agatgctctt gaaggagcag atatcttcat
    17821 tggcgtatca gcaccaggag tcttaaaagc agagtggatt agtaaaatgg cagcgcgccc
    17881 agtaattttt gccatggcca accctattcc agagatttac cctgatgaag cccttgaagc
    17941 gggggcttac attgtaggta ctggccgtag tgattttcct aaccagatta ataatgtcct
    18001 tgcctttcca ggtattttta ggggagcatt agatgcacgg gcaaaaacca ttactgttga
    18061 gatgcaaatt gctgccgcaa aaggtattgc cagtcttgtt ccaggtgacg ctttgagcac
    18121 tactaatatt attccagatg cctttaaaga aggagttgct gagattgtcg ctaaaagcgt
    18181 caggtcggta gtccttaaat catagaatat atgaaaaaaa gagaaagtta tactttaaat
    18241 cacaaaataa gtagaagcat atttctagct tctacttatt ttgtgattag ctaatatcat
    18301 tatcaataat aactttcaag acatttagta gcatttcagt tgtattggca ttaacaagtc
    18361 cagtgtttaa ggtgatattt ttaatccata attcatccag gttgaaatca actggtttcc
    18421 catgaacccc aacgttagca atatgcccac ctactgatat aattttttgg caaatatcaa
    18481 atgtcgcggg ataaccaaca cactccatgg aaatatcgac accacgtcca ttggttatat
    18541 cgtcaatgat agccttgact tcttcactac ttcctgaaca aatcgtgtgt gtggctccaa
    18601 acgttttagc tgcttcaagg cgattttgag ataagtcgac cataataata ttggctggag
    18661 agaaaagttg aacggttagt agggctgcga gtcctactgg accggcacca acaatacaaa
    18721 cattatctcc aggcttgaca tgtgatggca agacaccaat ttcatatgaa gttggtaaaa
    18781 tatcagaaag catgaccagt gcctcatcat caatcgtatc aggagcatga tagagactac
    18841 catctgcatg tgggatatgg acatattctg cttgtgttcc attaattaaa tggccaagaa
    18901 tccaaccgcc atcttcacaa tgtgacgaaa ggcctcgttt acaataataa caggtatgac
    18961 atgatgtgac gcaagaaata atcactttat ctccgatttt aaagttagtg accgcatcac
    19021 cgatctcctt aacaattcca attccttcag gaccaaggat tgttccttct ttacatgcgg
    19081 gaacatcccc acctaagata tgcaaatcag ttccacaaat agagtggttt ttaccaactg
    19141 gacgatatgt aggctttata atgactggtt ttggtttgtc tatgagctgt agatttccgg
    19201 ttgaaaggta agttgctgct ttcatattta actctttata aggttgagag gtaatcaacc
    19261 aattca
//
LOCUS       DATXIG010000030        18887 bp    DNA     linear   BCT 28-DEC-2023
DEFINITION  TPA_asm: Streptococcus pyogenes strain MGAS11113
            SAMN08555799-rid19682583.denovo.030, whole genome shotgun sequence.
ACCESSION   DATXIG010000030 DATXIG010000000
VERSION     DATXIG010000030.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN08555799
            Sequence Read Archive: SRR6740495
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pyogenes
  ORGANISM  Streptococcus pyogenes
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 18887)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 18887)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-OCT-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-OCT-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001918058.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001918058.2
            Genome Coverage       :: 368x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/12/2023 11:23:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 1,806
            CDSs (total)                      :: 1,769
            Genes (coding)                    :: 1,722
            CDSs (with protein)               :: 1,722
            Genes (RNA)                       :: 37
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1, 1 (5S, 23S)
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 47
            CDSs (without protein)            :: 47
            Pseudo Genes (ambiguous residues) :: 0 of 47
            Pseudo Genes (frameshifted)       :: 30 of 47
            Pseudo Genes (incomplete)         :: 20 of 47
            Pseudo Genes (internal stop)      :: 9 of 47
            Pseudo Genes (multiple problems)  :: 11 of 47
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..18887
                     /organism="Streptococcus pyogenes"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN08555799-rid19682583.denovo.030"
                     /strain="MGAS11113"
                     /serotype="Emm28"
                     /isolation_source="normally sterile site"
                     /host="Homo sapiens"
                     /db_xref="taxon:1314"
                     /country="Finland"
                     /collection_date="2002"
                     /collected_by="National Public Health Laboratory"
     gene            complement(59..820)
                     /locus_tag="VC122_001093"
     CDS             complement(59..820)
                     /locus_tag="VC122_001093"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018366755.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LPXTG cell wall anchor domain-containing
                     protein"
                     /protein_id="HEP5641024.1"
                     /translation="MTKEKMMSFMTIASIALLGSGVVCANDVADPVAGTTDAVVVPVS
                     PQPTTPIEHTGTDTTNKIVDSTKPSEDKVADSTDQLIPPVKSDLETSEDKVTDPTETK
                     PSKEKTEEPVKSNDSEVIPEQPKSEEVKKAEENTGASTTETPKPVTKPVIETPIVTNT
                     GYTVLGTQDGNVWVQTETGNVLKPAIAIGAVKQKDGTVALKTRDGKLEVLPSTGETKT
                     ILAILGGLAILGAIWVGWKENIKKYLTFLKKKSKK"
     gene            complement(845..1402)
                     /locus_tag="VC122_001094"
     CDS             complement(845..1402)
                     /locus_tag="VC122_001094"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015057923.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEP5641025.1"
                     /translation="MKIRKVIIVMLAVALSLGFYRLYHRQKPSQLIEANQEILVKPIT
                     PNKSKIKVKRNVAKEAKATLEMPEERVNEKEAKQVKSAIVIPIDYLASISSQDDIKVT
                     YDNKLSITNPAMAASIKTMLLAGYHFDLDSLTVYQSNSDNVYQFTILLVTHQQDQLSL
                     VGNYVTGTGQFEFVSLHGTPKNVMF"
     gene            complement(1402..2754)
                     /locus_tag="VC122_001095"
     CDS             complement(1402..2754)
                     /locus_tag="VC122_001095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018366753.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CHAP domain-containing protein"
                     /protein_id="HEP5641026.1"
                     /translation="MSIPFFIVVIVTIIFSSSQGDCQVTPDLSTQFVTSSDSASQTDW
                     TKKGSIANVTAGKVFHAWVSKGLSGASAAGIVGWVNSEGGFAMIGRAEGHYGNDLRTN
                     SIAFGVKPIGLSYYTTEAGGGIYQFTPYTKYAPLGDPKWENADAMNEFVAKAILAGDW
                     NASMDLTGGNHTFKQMAQMTDPKQATLVWQAYERGSTAHINPFQKQADAQKAYELFGG
                     VKYSYNEIVFHKSFDIFGPLKPGEGESKEVVLSLCESGSYSKGAFGKDRTGKVNYTSY
                     NAWRPEQLPADLKPYALDPRSVGLNYRQAVGWYAIASTGGQCTDLSASLMYALWSKNG
                     SHPTQKAGNGNMVVANWVRTFGGSSDKSPTSGAVFSSAGTSSAGHTGVVSHVFENGDI
                     LIVEQNYASYSGDDGGFGKYSWNYRYVTTAELKNEGYTFYNPAKLGYQIMKSVGTVGK
                     "
     gene            2895..3038
                     /locus_tag="VC122_001096"
     CDS             2895..3038
                     /locus_tag="VC122_001096"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004239191.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative holin-like toxin"
                     /protein_id="HEP5641027.1"
                     /translation="MGNSSKSNRKEELDLSTFEVLTLMFIAGNFVIALVKLVLELVKS
                     TKK"
     gene            complement(3206..3775)
                     /locus_tag="VC122_001097"
     CDS             complement(3206..3775)
                     /locus_tag="VC122_001097"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017646369.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEP5641028.1"
                     /translation="MGKHSPMNFSKPSQMTESIDHDVHYDLPGVNEEMESPFQSESDY
                     QSQNSSIIVETPEMDDMIYQSSQKKNISIGNNYSSDKKSLENSFDSPIIPERSEPETD
                     AKERFNPQAEYQRLKAKRQKPLGNRKKSKLEAELGQFTDDASYYKAHGGTAFKRGFNA
                     SKSKELRLKQNLERKATILAELDKLRGHV"
     gene            complement(4349..5521)
                     /locus_tag="VC122_001098"
                     /pseudo
     CDS             complement(4349..5521)
                     /locus_tag="VC122_001098"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015057927.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            complement(5536..8037)
                     /locus_tag="VC122_001099"
     CDS             complement(5536..8037)
                     /locus_tag="VC122_001099"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017646450.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HEP5641029.1"
                     /translation="MVKKLEYPINSIHSNLALRKDKVVIAYYRIPNTPITITDSDKKD
                     SHKDKVAQVIKKLAKYKHFDISLVPKDYLLEEKMSDFIGDLADDVKDLGIDTLSYTVD
                     TLTAEIEIPYQFDWVIGVNLGRDTMSATLKELAVKQLDLIADNVASLLGYTVELDKEW
                     YKEYQDEELLLYGLLSSLKAKRLSEEDLFYYQRMQYLRYIPHAKNEVIANRTLLNVTD
                     TLVKPLSGGFLKLESPYGSSFITILPVGKFNTIFNGFHLGELVQRMSFPVELRFKAEF
                     INKTKLSGTMGRSNTRYDQIMKEAYSTNTVQQDDILMGSYSLKDLMKKVGNKEEIIEY
                     GCFLVVSGSSIKQLRQRRHAVLSYFADMQVNVHEASHDTPYLFQTLLYGQNLQKTTRK
                     WNHLVTSRGFSEMMLFTNTQSGNRIGWYIGRVDNRLSAWDNIGEAIMGSKNLVLFNAT
                     VANKEDVAGKVTKNPHIIITGATGQGKSYLAQMIFLHTAQQNVRVLYIDPKRELRKHY
                     LQVVSDPEYAKQFPLRKHQIEQINFVTLDSAMKENHGVLDPIVILDKEDASSTAKNIL
                     LYLLRNATEVKIVQTTALTEAISEVISRRENGEVVGFNQVIETLCQSDNQDIVDVGHY
                     FKAIIKNSILELAFSDGNVKGLSYEERVTVLEVADLSLPKDGSDHISDHESNSIALMF
                     ALGAFCKHFGERTNEETVEIFDEAWILMQSSEGKAVIKSMRRVGRSKYNVLMLVSQSV
                     HDAENDDDTTGFGTIFAFYEKSEREAILTHVGLEVNEKNLEWIDNMISGQCLYYDVYG
                     NLNMMSVHNIHPDIDPLLKPMKQSVSSHLENKYAS"
     gene            complement(8058..8468)
                     /locus_tag="VC122_001100"
     CDS             complement(8058..8468)
                     /locus_tag="VC122_001100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017643201.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer protein"
                     /protein_id="HEP5641030.1"
                     /translation="MEEEKYFDYSRGLNAPYWIQEIKTSKGKLLWYFSTPMQLSFFIV
                     FFLILVLMLTVFSAPMQLLNQLTHSISLLLYWFVPYRLSKFYTEYEPQGKKMHTYLWD
                     YLVYLIDYGFNKKAIYQGERVEVLEEIVFEKTNI"
     gene            complement(8471..8740)
                     /locus_tag="VC122_001101"
     CDS             complement(8471..8740)
                     /locus_tag="VC122_001101"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001055037.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEP5641031.1"
                     /translation="MKVSKVAQVVLTGGGPNVSGLKQFFQGDGIYLISVICAFLVIKN
                     WKNADWLKVGSVLSIYAIVVSLFKGQEILAFLGGMLRWFGIETGL"
     gene            complement(8759..9709)
                     /locus_tag="VC122_001102"
     CDS             complement(8759..9709)
                     /locus_tag="VC122_001102"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000565541.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer protein"
                     /protein_id="HEP5641032.1"
                     /translation="MDYLVKIKKFLLRYTKKEKGGKQPKPKEVKQKTANIIVYGILGL
                     LFIVGFFGSLRAIGLSNQVNSLKATVLSSKQQNGKDTKEILDIPRVQYYMNNFVYTYI
                     NYSDDTATKRKEELDNYYSFSTANLVDDVKKERKLQTQRLVSVEQEEDYHIALMRIGY
                     EVDSKSYQMTLAIPFRMANGLLAIVSPPYTLAEDLFQGKSKSFERKTVDQVKSLPKQE
                     MLSIQKFLPVFFDKYALSNEIDLKLLMKHPELMGGNYRVKSLDANNARYYQDKSNQVV
                     QLMVTFEDMVTGGTRSENFTLYLIKSDNGWYVDKLYHYFK"
     gene            complement(9710..10189)
                     /locus_tag="VC122_001103"
     CDS             complement(9710..10189)
                     /locus_tag="VC122_001103"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011285002.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEP5641033.1"
                     /translation="MIAIIRTHEKGRSQFLCELDIMQFTENQVRDHMIERGIKDDAFF
                     ICGFSDWNVDRIMSLSEVYLLKRCIVGLYDGDDYIVQYLLKKGLSVPAIVTKFYVFLS
                     KDEKEAMGYVLKNVSFDSLIDFWQRSVTWANALNAYIQSGILLNTSKGFYVLKTEGG"
     gene            complement(10197..10678)
                     /locus_tag="VC122_001104"
                     /pseudo
     CDS             complement(10197..10678)
                     /locus_tag="VC122_001104"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000383283.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="antirestriction protein ArdA"
     gene            complement(10731..11003)
                     /locus_tag="VC122_001105"
     CDS             complement(10731..11003)
                     /locus_tag="VC122_001105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011285005.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEP5641034.1"
                     /translation="MSNTRSEADKKLLVVTQELSELLISHQYDQSWEKAGELNSLLKK
                     REELTLPGYMVDMIQQHLKSYYYQNNMINKAHKSMSAIGHKLQEFH"
     gene            complement(11136..11341)
                     /locus_tag="VC122_001106"
                     /pseudo
     CDS             complement(11136..11341)
                     /locus_tag="VC122_001106"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015057932.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
     gene            complement(11392..11769)
                     /locus_tag="VC122_001107"
     CDS             complement(11392..11769)
                     /locus_tag="VC122_001107"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000287184.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEP5641035.1"
                     /translation="MFLENEFNYFISTRNNLELVIDSLVLMIPDREFYYPEIQTGELR
                     DYQIDIYDLIKIGYVGVYEIQKDYEDKLRELADFKRKLLKFGLLMQPLEKQKEIVIRL
                     AAKYHLEKRILMKKEMFRDEEVD"
     gene            complement(11770..13011)
                     /locus_tag="VC122_001108"
     CDS             complement(11770..13011)
                     /locus_tag="VC122_001108"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001069008.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation factor domain-containing
                     protein"
                     /protein_id="HEP5641036.1"
                     /translation="MNPMYLKKFRKKSGLKQKEFAKCIGISQAMLSQYESGKKKLSLE
                     KFQEMKTHFGYLTDNDSSRLQVTIDYVRITLKSVRDLEFFCRNFLHCRFKEFSSVESK
                     LMNYNHIWKRGDIWIFDYADKHDTGNYQITLQLSGQGCRQLELLMEQENFAWLDWLIY
                     LRRSYRDEMNVTRFDLAIDELYLGKDRENEHFLLSDMIAKYYHHELAFESLRTWNYIG
                     GGALNFNDMEEIEQNRQGISLYFGSRQSEMYFNFYEKRYELAKLEGISVEESLEIFEI
                     WNRYEIRLSQSKANAVVDEFISGIPLGEIARGLINSKIQVYDGKNKYGAFMADQKWQM
                     MFGGVEPLTFVTRPESYNIERTIRWLIHQVSDSLAMVSEYDSMIDGDNLQMIINSGEV
                     NERGEKILETIRYNLGLDKGA"
     gene            complement(13323..15032)
                     /locus_tag="VC122_001109"
     CDS             complement(13323..15032)
                     /locus_tag="VC122_001109"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015057933.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsK"
                     /protein_id="HEP5641037.1"
                     /translation="MRLLPEYRGIKVKPYMRYMSYYLFSFLFILFLIPVGVYSYYHLD
                     LLKMMDKMTMIILGISLFLSLFVSWYLTWFLQEANPLFNKLDRLKRLAKFLFENGFVY
                     EKRKKVNKKQKVSFPKVYLKQGKFDLSVSFEMAGNKFQKRFKDIGGELEDTFFMDFME
                     KTDDPRFKIYKLAYSAFLARITVNDVVWEKDKGIKLMNGYYWDFINDPHLLVAGGTGG
                     GKTVLLRSILKCLATIGVADICDPKRADFVTMADLPAFRGRIVFEKVDIIAKFENAIT
                     IMYARYDFIRKEMKRLGHKDMGKFYDYGLEPYFFICDEYNALMSSLSYQERDIVENAF
                     TQYILLGRQVGCNAIIAMQKPSADDLPTKIRSNMMHHISVGRLDDGGYMMLFGDENRN
                     KEFRYIKFLSGHRVYGRGYSAVFGEVAREFYAPLLTKKFSFYDEFAKISRHDNPFDPL
                     ENTLVSKDIISDESLRKFVEKTSDNELPKGSIGFDFEEEMEEINHMSIGDLAKKTGFS
                     FGKIKNLIDMIEKDGYMTFNREDERVVLTDSDAVILNSLLTQKDKFDGSWKELLLNYF
                     KGE"
     gene            complement(15052..15507)
                     /locus_tag="VC122_001110"
     CDS             complement(15052..15507)
                     /locus_tag="VC122_001110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011285010.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer protein"
                     /protein_id="HEP5641038.1"
                     /translation="MAKVGLNFGEKIQIVDKSYRVLNDGLVDLKEIFGDLSFRSFEET
                     DLIYEDDMTQPRRQDGSYPQIPTGESRGLVVGIFSPVQQQTEFVTLVDMSLAEFEALQ
                     LKYREPVELEGIIVTYSSVGSNQFKLFASKIKRSVSQKVANEGNHKQEK"
     gene            complement(15510..15815)
                     /locus_tag="VC122_001111"
     CDS             complement(15510..15815)
                     /locus_tag="VC122_001111"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011285011.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytoplasmic protein"
                     /protein_id="HEP5641039.1"
                     /translation="MEKSTLKLKPDFIGKAWYEKLGNAPRRIDGEAGQFQRHIVSNEK
                     HGVFHVITPALAQVFKTDDVVEIDGEAIFFEDRALNGNSVAPALNVRATGLKLAGGK"
     gene            complement(16077..18569)
                     /locus_tag="VC122_001112"
     CDS             complement(16077..18569)
                     /locus_tag="VC122_001112"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008089048.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LPXTG cell wall anchor domain-containing
                     protein"
                     /protein_id="HEP5641040.1"
                     /translation="MTVTTQPDGSFVFKHDMIDGVKTIGHGTLTGKINHHYEANKDGS
                     ITAYIDSVTFDKYEYQNVAKNDAVDKNIAFRILSATGQELFVKAHDGDKTFSETLNKT
                     VSLKLTYQLKPHEAVKDIKVFQLHDDWVHDTHGSALVSYVNNNDAVPHIEVPEKPVEP
                     DMVTPKVEQEKPVPEAPQKPTDGVPNLEKEKPVPPTPVKPEAVKPVLEQEKPVPEAPQ
                     KPTDDVPNLEKEKPVPPTPVEPEAIKPDLQSFTPEVYDPIKPVVKPHVTVPEKVVYEV
                     MVHPVQVKQTPTNVKSVTNSDQVNIDGQLVPKGSTVTWELVNTSLKAGRQDITSYELT
                     DPLPDGFELDVTATQTLSPEWVITTDEAGKVSLKASQSLLAHFNAKRDQDVEVPKVSL
                     VGRLLNDAGTYHNTFKTVVTTPTGSYTVISNTPVIYTPGNDPKTPRNPGGDNPTPHDN
                     LIQPTKTIVDDKGQSIDGKSVLPNSTLTYVAKQDFDQYKGMTAAKESVMKGFIYVDDY
                     KDEAIDGHSLVVNSIKAANGDDVTNLLEMRHVLSQDTLDDKLKALIKASGISPVGEFY
                     MWVAKDPAAFYKAYVQKGLDITYNLSFKLKQDFKKGDITNQTYQIDFGNGYYGNIVVN
                     HLSELTVHKDVFDKEGGQSINAGTVKVGDEVTYRLEGWVVPTNRGYDLTEYKFVDQLQ
                     HTHDLYQKDKVLATVDITLSDGSVITKGTDLAKYTETVYNKETGHYELAFKQDFLAKV
                     VRSSEFGADAFVVVKRIKAGDVANEYTLYVNGNPVKSNKVTTHTPEQPQPVTPKAPAL
                     PSTGEQGVSILTALGAALLSLLGYVGLKKRQQ"
BASE COUNT         5882 a         3960 c         2744 g         6301 t
ORIGIN      
        1 ttttcaattt cgctcctttc aacaaaacta aagccatgaa attaatcatg gctttatctt
       61 attttttact tttctttttc aaaaacgtta aatacttttt gatattttct ttccaaccta
      121 cccagatagc tcctaaaata gctaaacctc ccaaaatggc taagattgtt ttagtctctc
      181 cagttgatgg taagacttct aacttcccat ctcttgtttt taaggcaact gtaccatctt
      241 tttgctttac agcaccaatt gctatcgcag gtttcagtac attaccagtt tctgtttgaa
      301 cccaaacatt gccatcttgc gttcccaaaa ccgtgtagcc tgtattcgta acaattggtg
      361 tctcaataac aggtttcgtc acaggtttag gagtttcagt tgtactagca cctgtatttt
      421 cttcagcttt tttcacctct tctgacttag gttgttctgg aatcacttct gaatcattgg
      481 acttaacagg ctcttccgtc ttctctttgc ttggcttagt ctccgttgga tctgttactt
      541 tatcctcgct tgtttctaaa tctgatttaa ctggagggat tagctgatca gtactatctg
      601 ccaccttgtc ctcgcttggc ttagtcgagt caacaatttt attggtagta tctgttcctg
      661 tatgctcaat cggtgtcgtt ggttgaggtg agactggtac caccactgca tccgttgttc
      721 ctgctactgg atctgctaca tcattagcac aaaccacccc acttccaagc aaagcaatac
      781 ttgcaatcgt cataaaactc atcatttttt cttttgtcat cttatttttc ctcttttcgt
      841 ttaattaaaa catgacattt tttggagtgc catgtaaact cacaaattca aactgacccg
      901 taccagtaac ataattacca acaagactta attgatcttg ctgatgagtc actaataaaa
      961 tcgtaaattg atacacatta tcagaattgc tctgataaac cgtcaagcta tctaaatcaa
     1021 agtgataccc tgctaaaagc atcgttttaa tgcttgctgc cattgctgga ttagtaatgg
     1081 acaacttatt atcataagta accttaatat catcttgact tgatatacta gctaaataat
     1141 caatcggtat tacaatagct gatttcacct gtttagcttc tttttcgtta acacgctcct
     1201 caggcatctc taaagtggcc ttggcttctt tggcaacatt tctcttaacc ttaattttac
     1261 ttttattcgg tgtaattggt tttacaagta tttcttgatt agcttctatt aactgagatg
     1321 gcttttgacg atgatacaac cgataaaatc ctaatgaaag agctactgcc agcataacaa
     1381 taataacttt tcttatcttc attatttacc taccgttcct actgatttca taatctgata
     1441 accgagtttc gcaggattgt aaaaagtata tccttcattt tttaattctg cagtagtgac
     1501 atagcgatag ttccaagagt atttcccaaa gccaccatca tcacccgaat aagatgcata
     1561 attttgttcc acaattaaaa tatccccatt ttcaaaaaca tggctgacaa cacctgtatg
     1621 gccagcagaa ctagtccctg cagaggaaaa tacagctcct gaagttggtg atttatcaga
     1681 tgaaccgcca aaagtcctaa cccaattagc aacaaccata ttaccattac ctgccttttg
     1741 tgttggatgg ctaccatttt ttgaccacaa agcatacatc aaactcgcag aaagatcggt
     1801 acattgaccc cctgtcgaag caatcgcata ccagccaacc gcttgtcgat aattcaagcc
     1861 aactgatctg ggatcgagcg cataaggttt taaatctgca ggcaattgtt ctggtcgcca
     1921 agcattgtag gaagtatagt tgactttgcc tgtacgatct ttcccaaaag cccccttaga
     1981 gtaagaacct gattcgcata atgataaaac gacttctttt gattcccctt cccccggctt
     2041 tagtggacca aaaatgtcaa aagatttatg aaatactatt tcattataac tatatttaac
     2101 tccaccgaat aactcatacg ctttttgagc atctgcttgc ttttgaaatg gattaatatg
     2161 ggcagtacta ccacgttcat aagcttgcca aactagcgta gcctgtttcg gatcggtcat
     2221 ctgggccatt tgcttaaagg tatgatttcc tcctgtcaaa tccattgaag cattccaatc
     2281 accagctaga atggcttttg caacaaattc gttcatggca tccgcattct cccacttagg
     2341 atcacctaaa ggggcatatt tggtataagg ggtaaattga taaatacctc cacctgcttc
     2401 tgtcgtatag taactcaaac caattggttt cactccaaaa gctatagaat tagttcgtaa
     2461 atcattacca taatgtcctt ctgctcgacc tatcatggca aaaccacctt cggaattaac
     2521 ccaacctaca attcctgcgg cagaagcgcc tgacaagcct ttcgataccc aagcgtgaaa
     2581 gactttacct gctgtgacat tcgcaattga gcctttcttt gtccaatcgg tttgtgaagc
     2641 agaatcactt gaggtaacaa attgtgttga taaatcagga gttacttgac aatcaccttg
     2701 actgctgcta aatatgatcg taacgataac tacaataaaa aaaggaatgc tcataagcac
     2761 tcctagacat ccaagtttct tcttgataac attccctcct tcttaaactg gtcagacaga
     2821 gatacggtat gttataataa aaaggcagaa aagttaagac ggtggctctt atttctgaaa
     2881 ggtaggtggt gcccatgggc aattcatcaa aatccaacag aaaggaggag ctagatttgt
     2941 ctacctttga ggtcttaaca ctcatgttta tagcaggtaa cttcgttatc gctctcgtga
     3001 agttagtcct tgaactggta aaatcgacaa aaaaataacc gtctcaactt tggcgagtta
     3061 acggttattt tttaacggtt attttctgcc accgtcttaa acggttctgc attgggtagt
     3121 tgtttccgca actgcccttt tctattttca ttataactct tatctccaaa ttaagcaact
     3181 atttgataac atcacaatac ttcctttaaa catgtcctct aagtttatct aattcagcta
     3241 aaatagtcgc ctttctttct aaattttgtt ttaaccgtaa ctcttttgat ttactggcgt
     3301 taaatcctct tttaaaggca gtccctccat gtgctttata atagctggca tcatcagtaa
     3361 actgacctaa ttctgcttct aatttagatt ttttccgatt tcctaaaggt ttttgtcgct
     3421 tagcttttag acgttgatat tctgcttgag gattaaatct ctctttagcg tccgtttctg
     3481 gttcagacct ttcaggaata attggagaat caaatgaatt ttccaaagat tttttatcag
     3541 aggaataatt atttccaata gaaatatttt tcttctgact gctttgataa atcatatcat
     3601 ccatttctgg agtttcaacg ataattgaag agttttgaga ttgataatca ctttctgact
     3661 gaaatggact ttccatctcc tcattaacac ctggtaaatc ataatgaaca tcatgatcaa
     3721 ttgattcagt catctgactt ggtttagaaa aattcattgg ggaatgttta cccaagggtt
     3781 ctgaattatc taatgaagct ccaaaagatt ggctgtataa gttgtctctt tcaaaatcac
     3841 ttacttgaga gagattgtaa tcagaagaaa agttactctc ttgattatcg aaaacacctt
     3901 gttctgcttg atgtccagag gtaggattac cattatcaga aacatgcggg acactatttg
     3961 tggaaaattc ttgatctcct gcaaaatctc ctaattctga aaactgatgg tcgaaattag
     4021 tttcattaga aaggtcacta taattagaag tgtcatctcc tttgaaacgg tttttaatgg
     4081 cactaagatt atccttagca tgtacaaggc taccaacacc ataaccagct cccatcatcg
     4141 caagattaga aatatcatgc gccttatcat agatattttg gggtgtaact cctaaactat
     4201 tcgctttttg agcaaccata tttcctgctt gtgtgactgc ttgtgcagcg tctgctccta
     4261 caattaaact taataccttt tctttatgtt tccaaatttg ccaaaaaata accccctgaa
     4321 caacaatagt aataaacaac ataaataaaa tagattgtcc cttaaggtta ctgctgctta
     4381 acaaatgatt gtctgtaaat cgagacttaa taaatgtcgc aatcaatgac tgtatataaa
     4441 agactattaa agttaaaaaa cctgttagag ctggaaagga aacacctcca agcattagct
     4501 taagcatatt aaaagcaata tgttgcattt taggtaaaaa ggacaccaat aaagccagtg
     4561 ggaataaaaa cattaacaat atgaccacaa tatctgctaa aaaagcaatt aattgtatca
     4621 ataataaagg tatagctaat atcactgctt caaaaatttt caaaataaca taaatagact
     4681 tgatccaaat atagtcacta acagctgaca tccacctgtt ttgttcttct cctttcaaag
     4741 ctccgtcacc aagtttattt gtatactcta atatttgttg actttttggt attacaaact
     4801 gatttgactt atcatatttt cccagaattt tgctattatc taactcctct tcttttcctg
     4861 atttattatt atgaaactta ccatttaact ggccagtatt tacaaaaaga taagctgtat
     4921 aagacgtttt cataacataa ttatcagcta ccgaagcctc atgagaaatg atttgcttct
     4981 catcttctgg actcactacc gatagattag ttactgcatc actaaaagaa ctagctaccg
     5041 aatgaaccat atccaacaca tagacaccgc ccgaagttga ctgcaccgtc ccaaagtaac
     5101 cgaacgctaa taacataacc gcaaacaaat gaagtaatcg tcttgaaaaa tcaccttttg
     5161 aaaaaataaa agctagaaaa atggaaaaag cggataaggc aattaagaga taaagtaatg
     5221 aattcgtaaa atctgcggat ttagagaggt tatcccacaa aactttggaa ctatcaaaca
     5281 cagtctgacg ataagtatta tacaagctaa agttttcaaa aaatcgtaga ataatggaaa
     5341 agaaaccaac aatcaagtta agtagaaaaa agggaaaatt aacaaaaata tctgtcaaag
     5401 ttacttgtaa ccccgtctca agaccttctg tactttccat gtaagccttt aatgcaaaaa
     5461 tatccacatc cttgactttc tgaaaggtag aagctattaa tccatcatca atcttatcca
     5521 tttggcctct cctttctacg aagcgtattt attttctaag tggcttgaaa cagactgttt
     5581 cattggtttt aataagggat caatatctgg atgaatgtta tggacagaca tcatattgag
     5641 gttgccataa acatcataat aaaggcattg tcctgaaatc atgttatcaa tccactccag
     5701 gttcttttcg ttcacttcca aaccaacatg agttaaaata gcttcccgtt ctgatttttc
     5761 gtaaaaagcg aaaatagtac caaaaccagt cgtatcgtca tcattttcgg catcatgtac
     5821 tgattgagac actaacatta aaacattgta cttagaacga ccaacgcggc gcatagactt
     5881 gataacggcc ttaccttctg atgactgcat cagaatccaa gcctcatcaa aaatttcaac
     5941 agtttcctca ttggttcgtt ccccaaagtg cttacaaaaa gcacctaaag caaacattaa
     6001 agcgattgaa ttactttcat gatcagatat atgatctgaa ccatctttag gtaacgataa
     6061 gtccgcaact tctaaaactg tcacacgctc ttcatatgat aatcctttaa cattaccatc
     6121 cgaaaaagcc aattccaaaa tagaattctt aataatagct ttaaaataat gtcctacatc
     6181 tacaatatcc tgattatctg attgacataa tgtctcaatg acttgattaa agccaacgac
     6241 ttccccattt tcacgtcttg agattacttc actaatggcc tctgtcaaag cagtagtttg
     6301 tacaatctta acttctgtcg catttcttaa cagataaagc aatatatttt tagcagtaga
     6361 cgaggcatct tctttatcca agataacgat aggatctaaa accccatggt tttccttcat
     6421 cgcactatcc aatgtcacaa aattaatttg ctcaatctga tgttttctca gtggaaattg
     6481 tttcgcatac tcgggatcag acaccacttg taaatagtgt ttccttaact ctctcttagg
     6541 atcaatataa agcactctaa cattttgttg cgcagtatgt aaaaaaatca tctgtgcaag
     6601 gtaggacttc ccctgacctg tcgcacctgt aatgataata tgaggatttt tagttacttt
     6661 accagcaaca tcctccttat tcgcaaccgt tgcattaaac aaaactaagt tcttcgagcc
     6721 cataatcgct tcaccaatat tatcccaagc agataaacga ttatcgactc gaccaatata
     6781 ccagccaatc ctgttacctg actgggtatt tgtaaataac atcatttcac taaacccacg
     6841 actggtcact aagtgattcc atttacgtgt tgtcttctgt aaattttgac cataaagtaa
     6901 cgtttggaat aaataaggtg tatcatgact agcttcatgt acattgactt gcatatccgc
     6961 aaagtaactt aaaacagcat gcctacgttg ccttaattgt ttgatactag acccagaaac
     7021 caccaaaaaa caaccgtatt caatgatttc ttctttattt ccaactttct tcatcaagtc
     7081 ttttaatgaa tatgagccca ttagaatatc atcttgttgc actgtattcg tggagtaggc
     7141 ttctttcata atttgatcat aacgtgtatt cgagcgtccc attgttccac ttaacttagt
     7201 tttattaata aattctgctt taaatcgtaa ctcaacagga aaactcatcc gctgtacgag
     7261 ttcacctaag tggaacccat tgaagatagt attaaacttc ccaactggta gaatcgttat
     7321 aaatgaacta ccatacggac tttctaactt taaaaaacct cccgacaggg gcttaactaa
     7381 agtatctgtt acgttcaaca atgttctatt ggcaataact tcatttttag catgtgggat
     7441 atagcgtaag tattgcatgc gttgataata aaataaatct tcttcagata atctcttagc
     7501 tttcagtgat gataacaaac catacagcaa taattcttca tcttggtact ctttatacca
     7561 ctccttatca agttccacag tgtatcccaa caaagaggcg acattatctg caattaaatc
     7621 caattgcttt accgcaagtt cttttagtgt agcactcatt gtatccctac ctaaattaac
     7681 accaataacc caatcaaatt gataaggtat ttcaatctca gctgtcaacg tgtcaacagt
     7741 gtaactcaat gtatctatac ctaaatcctt aacatcatct gccaagtcac caataaaatc
     7801 agacattttt tcctctaaaa ggtaatcttt tggtaccagt gaaatatcaa aatgcttgta
     7861 tttagctaat tttttgataa cctgtgctac cttgtcttta tgactatctt ttttatcact
     7921 atcagtaata gtgattggcg tatttggaat ccgatagtag gcaataacca ctttatcttt
     7981 tcgcaaagct aaattactgt gaatgctatt aattggatat tccaactttt ttaccatctg
     8041 atctcctttc ttaatcttta aatgtttgtt ttctcaaata caatttcttc tagtacctct
     8101 actctttctc cctgataaat agctttttta ttaaaaccat aatctattaa atagacaaga
     8161 taatcccaaa gataagtatg catcttttta ccttgtggct catactccgt ataaaatttt
     8221 gaaagtcgat atgggacaaa ccagtacagt aacagagaaa tagaatgcgt tagctgattt
     8281 agcagttgca taggagccga aaagactgtc aacatcaata ctaaaatcaa aaagaaaaca
     8341 ataaaaaagg aaagctgcat tggtgttgag aaataccaca ataactttcc ttttgacgtt
     8401 tttatttctt gaatccagta aggggcgttt aggcccctac tgtaatcaaa atatttttct
     8461 tcttccattt ttataaacct gtctcaatac cgaaccagcg aagcatccct cctaaaaagg
     8521 caagaatttc ttgcccttta aacagagaaa ccacaatcgc ataaattgac agtactgagc
     8581 caacttttag ccaatctgca tttttccaat ttttaatcac taaaaaagca cagataacac
     8641 taataagata aatcccatct ccctgaaaaa attgttttag tcctgaaaca ttaggtcctc
     8701 ctcctgttaa aacaacttga gctacttttg atactttcat taattaaacc tcgctttttt
     8761 atttaaaata atggtacaac ttgtcaacat accaaccatt atctgactta atcagataca
     8821 gcgtgaaatt ctctgaccgt gtacctcctg ttaccatatc ttcaaaggta accattaatt
     8881 gcacaacttg atttgattta tcttgataat accgagcatt gttagcgtct aaagatttga
     8941 ctcgatagtt tccacccatc agttcaggat gcttcattag taacttcaga tctatttcat
     9001 tacttaaagc atacttatca aaaaatacag gtaaaaattt ttgtattgat aacatttctt
     9061 gcttcggtaa actcttgact tgatccacag tttttctttc aaaacttttt gatttccctt
     9121 ggaaaagatc ctccgctaag gtatatggag gacttactat tgccaataat ccattagcca
     9181 tacgaaatgg gatagccaac gtcatctgat aagatttact atctacctca tagcctattc
     9241 tcattaaagc aatatgataa tcctcttctt gttctacact aaccagtcgt tgagtctgaa
     9301 gcttacgctc ctttttaaca tcatcaacaa gattagctgt tgaaaaagaa taataattat
     9361 ctaattcttc tttacgtttt gtagccgtat catcagaata gttgatgtaa gtatacacaa
     9421 aattgttcat gtaatactgc actctaggaa tgtctaagat ttcctttgta tcttttccgt
     9481 tttgttgctt gcttgataaa accgtagctt ttaaactatt tacttgattt gacaaaccaa
     9541 ttgcccttaa tgagccaaaa aatcctacaa taaataataa tcctaaaatt ccatagacaa
     9601 taatattggc tgtcttttgt tttacttcct ttggtttagg ctgctttcct cctttttctt
     9661 tctttgtata tctcaataga aactttttta ttttcactaa ataatccatc taacctcctt
     9721 ctgtttttaa cacataaaat cctttggatg tatttaacaa tattccactt tgtatgtaag
     9781 cgtttaaagc atttgcccaa gttactgacc gttgccaaaa atcaatcaat gaatcaaatg
     9841 atacattttt caaaacatat cccattgctt ctttttcatc ttttgacaaa aagacataaa
     9901 actttgtcac tatggctggc actgacaagc ctttctttaa caaatattga acaatgtaat
     9961 catcgccatc ataaagacca acaatacatc tctttaaaag gtaaacttcc gaaagtgaca
    10021 taatcctatc aacattccaa tcagaaaaac cacaaatgaa aaaggcatca tccttgatac
    10081 ctctttctat catgtgatct cttacttgat tttccgtaaa ctgcatgatg tctaactcac
    10141 ataaaaattg acttctacct ttttcatgag ttcttataat tgcaatcatt atctacttac
    10201 cctgatattt cgacatatcc gtagtctgaa aaaccacctt ggctttctaa gtcaatatct
    10261 cgaccataag cttcacaatc aaaatagagt tccatatttt ctttaccaac accttctgaa
    10321 cctaattcat taaaccagta ttcgcctaat tctcgtctag ttgtgacatc aggcagtaaa
    10381 caacaatcac caaaattaaa atcataatct acaaagtctg aggcagtata acctgttacc
    10441 tgatgcactg cttgaacaag ttcactatca acattctcta ccagttcaac aaattattaa
    10501 tcatctcaat agaatcatgt tcttttagta actgacaatc cgaatctgca ataatatact
    10561 ctaaatcatc ttcttcacga agccccaaag ctaacttaag ctcactcatt tccacaggaa
    10621 aacaaactaa cgtagctcta ccagttttta tatctctaac aattgcacta acatccattt
    10681 ttgacactcc ttaattaaaa aaggagcaag ccattgactt gctctcatcc ttaatgaaat
    10741 tcttgtagtt tatgaccaat cgctgacata gacttatgag ccttattaat catattattt
    10801 tggtagtagt atgatttcaa atgttgttga atcatgtcga ccatataacc tggtaaggtc
    10861 aattcttcac gttttttaag taagctattt aactctccag ctttttccca tgactggtca
    10921 tactgatgac taatcagcag ttctgatagc tcctgagtaa ccactaacaa ctttttatct
    10981 gcttctgaac gtgtatttga catttttctt ttcctctttt tcttttttta tactcaatac
    11041 caataaatca acatatgata gttggacacc taattttcat cattttccct cataaaaaag
    11101 aacctcttaa ggttcatgaa tctaaaacta actgtttaag catctgaaat acctaataaa
    11161 taatttggag tagtatttaa tagtttagat agctcaatta gttttgaaaa cttggttctc
    11221 gctttccgtt ctcccaattt gaatatgctc ctctattaat accaattcta tcagcaagct
    11281 cttgttgtgt taatttatta agctgtcgtt gttttcttaa acgatctcca agtttttcca
    11341 tttaccttac cccttatgat ttataagttg gattttaaat gatcccagaa attaatcaac
    11401 ttcttcatct ctaaacattt cctttttcat cagaattctc ttttccaaat gatatttagc
    11461 agctaaacga ataactatct ctttttgctt ttctaaaggt tgcataagca aaccaaactt
    11521 taaaagtttc cgtttaaaat cagcaagttc ccttaactta tcttcgtagt ctttttgaat
    11581 ttcataaaca ccaacataac caatcttaat caaatcataa atgtctatct gataatctct
    11641 aagctcacct gtttgaatct caggataata aaattcacga tcaggaatca ttaataccaa
    11701 agaatcaatg accagctcta aattatttct cgttgatata aaataattaa attcattttc
    11761 taaaaacatc tacgccccct tatctaaacc tagattatat cgaatagttt ctaatatttt
    11821 ttcacctcgt tcattcactt caccagaatt aatgatcatt tgtagattat ccccatcaat
    11881 catactgtca tattcactaa ccatagctag agaatcagat acttgatgaa tcaaccagcg
    11941 aatagtacgt tcaatattat aagattctgg tctcgtgaca aatgttagag gttcaacacc
    12001 tccaaacatc atctgccact tttgatcagc cataaatgca ccatacttat tttttccatc
    12061 ataaacttga attttactat taatcaatcc tctcgcaatt tctcctaatg gaataccaga
    12121 aataaattca tctaccactg cattcgcctt agattgagat aatcggattt cataccgatt
    12181 ccatatttca aatatttcta aagattcttc aacagagatg ccctctaatt tagctaattc
    12241 ataccttttc tcataaaagt taaaatacat ttcagactgt cgactaccaa aatataacga
    12301 aataccttga cgattctgtt caatttcttc catatcatta aaatttaaag caccaccacc
    12361 aatataattc catgttcgca aactttcaaa agctaactca tgatgataat atttggcaat
    12421 catatccgat aaaagaaagt gttcattttc tctatcctta cccagataca attcatcaat
    12481 agctaagtca aaacgtgtaa cattcatttc atctcgataa gaacgtctta agtagattag
    12541 ccaatctaac caagcaaaat tttcttgttc cataagtaat tctaattgtc tacatccttg
    12601 accagatagc tgtaatgtta tttgatagtt tcctgtatca tgtttatctg cataatcaaa
    12661 tatccaaata tctccacgtt tccaaatatg gttataattc atcaacttag attcaactga
    12721 tgaaaattct ttaaatcgac aatgtaaaaa attccgacaa aaaaattcca aatctctaac
    12781 gctttttaac gtaatcctga catagtcaat tgtcacttgt aatcttgaac tatcattatc
    12841 agtcaaataa ccaaaatgtg ttttcatttc ttgaaatttt tcaagagata attttttctt
    12901 accagattca tattgagata acatagcttg actaatacct atacattttg caaattcttt
    12961 ctgctttaag cctgatttct tcctaaattt ctttaaatac attggattca tctattttct
    13021 ccctaaatat tatcaatttt tagctatttt ttgataaaat ataacctgtt tttgctcgtt
    13081 ttataaccta tttttgaaac ttgaaaatcc ttgatacata tggggtttcg ggggttttac
    13141 ttcgtagatt tttcaaatct taccccccta ttagaaatcg ggggttttaa aattgataaa
    13201 tccctctgta aagccctagt gtgtgcgctg tgtccggcgt gtccgtttcc gctacgctac
    13261 actacgccta cagctacact agggctttac agagggatat ttaaaaaaat acccttcatc
    13321 tactattcac ccttaaaata attcaacaac aactctttcc aactgccatc aaacttatct
    13381 ttttgagtga gtaaactatt caatataact gcatcagaat cagtcaatac tactctttca
    13441 tcttcacgat taaaagtcat gtaaccatct ttttcaatca tatcaattaa attttttatt
    13501 ttaccaaatg aaaaacctgt tttcttagct aaatcaccaa tactcatatg attaatttcc
    13561 tccatttcct cttcaaaatc aaagccaata cttcctttag gtaactcatt gtcagacgtt
    13621 ttttctacaa attttctcaa tgattcatct gaaataatat ccttggaaac caaagtattt
    13681 tcaagcggat caaatggatt atcatgacgt gagattttcg caaattcatc ataaaatgaa
    13741 aatttcttag ttagcaaagg agcataaaac tctctcgcta cctcgccaaa aacagcagaa
    13801 tagccacgac catacacacg gtgacctgat agaaatttaa tatacctgaa ctctttattc
    13861 cgattttcat ccccaaaaag catcatataa ccaccatcat caaggcgacc aacagaaata
    13921 tggtgcatca tatttgacct gatttttgtt ggtaaatcat ctgcggatgg cttttgcatg
    13981 gcaataatcg cattacagcc tacttgacgg ccaagtagaa tatactgtgt aaaggcattc
    14041 tcaacaatgt ctctttcctg atacgataac gaagacatca aggcattata ttcatcacaa
    14101 ataaaaaaat atggttctaa tccgtaatca taaaatttcc ccatatcttt atgccctaaa
    14161 cgtttcattt ccttacgaat aaagtcatag cgtgcataca tgattgtaat tgcattctca
    14221 aatttagcaa taatatctac tttttcaaaa acaattcttc ctcgaaaagc aggtaaatca
    14281 gccattgtca caaaatcagc tcgcttagga tcacatatat ccgcaacgcc aatagttgca
    14341 agacatttta aaattgatct aagcaaaaca gtcttaccgc caccagtccc acccgcaaca
    14401 agcaaatgag gatcattgat aaaatcccaa taataaccat tcatcagctt aatgccttta
    14461 tccttttccc atacaacatc attaaccgtt atacgagcca gaaaggcgga ataagctaat
    14521 ttataaatct taaacctagg atcatcagtt ttttccataa aatccataaa aaaagtatct
    14581 tcaagttcac ctccaatatc tttgaaacgc ttttgaaatt tgtttcctgc catttcaaag
    14641 gagacactta aatcaaactt accttgtttt aaataaactt ttggaaaact aactttctgc
    14701 ttcttattaa cttttttacg tttttcataa acaaaaccat tttcaaataa aaatttagcc
    14761 aaacgcttta aacgatctaa cttattaaat aatggattag cttcttgtaa aaaccaagtc
    14821 aaataccaag aaacaaaaag cgataaaaac aaacttatcc ctaaaattat catagtcatt
    14881 ttatccatca tttttaacaa atctaaatgg tagtaactat aaacccctac aggtatcaaa
    14941 aacaaaataa atagaaaaga aaaaaggtaa taactcatat acctcatata tggttttacc
    15001 ttaatccctc tatattcagg cagtaaacgc atcttacctc tcccttatta tttatttttc
    15061 ttgcttatga ttaccttcat tagctacttt ttggctaaca gagcgtttaa ttttggaagc
    15121 aaataactta aactgattac ttcctacaga agaataagtc acgataatac cttctaattc
    15181 aactggctca cgatatttca actgcaacgc ttcaaactcc gcaaggctca tatcaaccaa
    15241 agtaacaaat tctgtttgtt gctgcactgg tgagaaaata ccaacaacaa gtccacgtga
    15301 ttcgcctgta ggaatttgtg ggtaagaacc atcttgacgt cttggttgag tcatatcatc
    15361 ttcataaatt aaatctgttt cttcaaagga tcgaaatgat aagtcaccaa aaatttcttt
    15421 caagtcaact aaaccatcat ttaatacgcg ataactttta tctacaatct gaattttctc
    15481 tccaaaattt aaacctactt ttgccattac tatttacctc cagctaattt caatcctgtt
    15541 gcacgtacat taagtgcagg tgctacacta ttaccattca aagctctatc ttcaaaaaag
    15601 atagcttcac catcaatttc aactacatca tctgttttaa atacttgggc taaagccggt
    15661 gtaatcacat gaaaaacccc atgcttttca ttcgacacaa tatgacgctg aaactgccct
    15721 gcttcgccat caatccgacg gggagcattt cctaattttt cataccatgc tttaccaata
    15781 aaatctggtt ttaactttaa tgttgatttc tccataattt acctcttttc atttttaaaa
    15841 taaaaaggct aggattgtct tatcaattat ttcctagcct taccacttag ttttttgact
    15901 tttcggtcac tattctagtg ataaaccaag atctatccac tcaccctaac aagtaaccaa
    15961 tagtaaactt tttcggctag ttttttgaag ggaaaggaga tccccctaat agttacttgc
    16021 tagagagaat ggatagaccc aaagtaatct actttgatgg cttatccatt aataacttac
    16081 tgttgacgct ttttaagccc tacataacct aagagtgaga gtaaagcggc cccaagcgcc
    16141 gttaagatag atactccttg ctctcctgtg gatggtaaag ctggtgcttt aggagttaca
    16201 ggttgaggtt gttctggcgt atgagtggtg actttattag acttcacagg attaccgttg
    16261 acatacaagg tatactcatt tgccacatca cctgctttga tgcgtttaac aaccacaaaa
    16321 gcatctgcac caaattcgct agaacgaacg accttagcta agaagtcttg cttgaacgct
    16381 aattcatagt gacctgtttc cttattgtaa actgtctcag tatatttcgc taagtcagta
    16441 cctttagtga taacactacc atctgataaa gtaatatcaa ccgtcgcaag gaccttgtct
    16501 ttctggtaga ggtcatgcgt atgctgaagc tgatccacaa atttgtattc tgtcaagtca
    16561 tagccacggt tagtcggaac aacccaacct tccaagcgat aggtgacctc atcaccaact
    16621 ttaacagtgc cagcattgat tgattgacca ccttctttat cgaaaacatc cttatgaaca
    16681 gttagttcag acagatggtt taccacgata ttaccgtagt atccgtttcc aaaatcaatt
    16741 tggtaggttt ggttagtaat gtcacctttc ttaaagtcct gtttaagttt aaaggacaag
    16801 ttgtaggtaa tgtctaaccc tttttgaaca taagctttgt aaaaggctgc tgggtcttta
    16861 gctacccaca tgtaaaattc accgactgga gaaatgcctg atgccttgat gagtgctttt
    16921 agcttatcgt caagcgtatc ttgagataac acatggcgca tctctaagag attggttaca
    16981 tcatctccgt tagcggcctt aatggaattc acaacgagtg aatgaccatc tatcgcttca
    17041 tccttgtaat catcgacata gataaagcct ttcatgacac tttctttggc agctgtcatc
    17101 cctttgtact gatcaaaatc ttgcttagcc acgtaagtca gcgtgctgtt tggcaagaca
    17161 gacttcccat caatggactg acccttgtcg tccacaatgg ttttagttgg ctgaatcaga
    17221 ttatcatgcg gtgttggatt atctcctcct gggttacgag gtgttttcgg atcgttccct
    17281 ggtgtataga tcactggtgt gttagagatt accgtgtagc taccagttgg agtagtgacc
    17341 accgtcttaa aggtattgtg ataagtcccc gcatcattca gtaaacgacc aaccagtgat
    17401 accttaggga cttctacatc ttgatcacgt ttagcattga agtgagccaa aagggattga
    17461 gaagccttaa gactaacctt gcctgcttca tccgttgtta tcacccattc aggagaaagg
    17521 gtttgtgtgg cggtaacatc taactcaaaa ccatctggta gtggatcagt tagttcatag
    17581 cttgtgatgt cctgacgacc agctttgaga gaagtattaa caagctccca cgttaccgtt
    17641 gatccttttg gtaccagttg tccatcgata ttaacctgat cactattagt aacagactta
    17701 acatttgttg gcgtttgttt cacttgaaca ggatgaacca taacctcata gacgaccttt
    17761 tcaggaacgg tgacatgggg tttcacaact ggtttaatcg ggtcataaac ctctggcgta
    17821 aagctttgga gatctggctt aattgcttca ggttcaactg gggttggtgg tactggtttt
    17881 tccttctcta agtttggaac atcatctgtt ggcttttgag gagcttctgg gactggtttt
    17941 tcttgttcta aaacaggttt aactgcctct ggtttaactg gagttggtgg taccggcttt
    18001 tccttctcta agtttggaac accatctgtt ggtttttgag gagcctctgg gactggtttt
    18061 tcttgctcaa cttttggtgt aaccatatca ggctccactg gcttttctgg tacttcaata
    18121 tgtggcacag catcattgtt gttcacataa gaaactaaag cagaaccatg tgtgtcgtgt
    18181 acccagtcat catgcagttg aaatactttg atatctttta cagcttcatg cggttttagt
    18241 tggtaagtta acttaagaga cactgtctta tttaaagttt cactaaaagt tttatcacca
    18301 tcatgggctt taacaaataa ttcttgacct gtggcactta aaatacgaaa agcaatattt
    18361 ttatcaactg cgtcattttt agctacattt tgatattcat acttatcaaa tgttacacta
    18421 tcaatataag ctgtaatgct accatcttta ttagcctcat aatgatgatt aattttaccc
    18481 gttaaagtcc catgaccaat tgttttgaca ccatcaatca tatcatgctt aaaaacaaac
    18541 gaaccatctg gttgcgttgt aacagtcatg attgaattac catattctgc attatcaaca
    18601 ccgttggctt ttgcatcaat tccaaagaca acctgatcga tagctttgtt ttcagcttct
    18661 tttttagctt gtagtgcttg ttggtaggct gataattgag cttcataagt agtttttaac
    18721 tgttggtttt gacgttctgc ttcttgtcga gctttagtgt tgcgatcacg aatggcggca
    18781 ttctcggcgt taatacgctc aatctctgct agcttttctt gataggcagc ttgagccgct
    18841 tgattcgttt gattcgtcgt ttgacctgct tgttcattac gcttacg
//
LOCUS       DATXIG010000031        17916 bp    DNA     linear   BCT 28-DEC-2023
DEFINITION  TPA_asm: Streptococcus pyogenes strain MGAS11113
            SAMN08555799-rid19682583.denovo.031, whole genome shotgun sequence.
ACCESSION   DATXIG010000031 DATXIG010000000
VERSION     DATXIG010000031.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN08555799
            Sequence Read Archive: SRR6740495
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pyogenes
  ORGANISM  Streptococcus pyogenes
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 17916)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 17916)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-OCT-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-OCT-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001918058.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001918058.2
            Genome Coverage       :: 368x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/12/2023 11:23:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 1,806
            CDSs (total)                      :: 1,769
            Genes (coding)                    :: 1,722
            CDSs (with protein)               :: 1,722
            Genes (RNA)                       :: 37
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1, 1 (5S, 23S)
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 47
            CDSs (without protein)            :: 47
            Pseudo Genes (ambiguous residues) :: 0 of 47
            Pseudo Genes (frameshifted)       :: 30 of 47
            Pseudo Genes (incomplete)         :: 20 of 47
            Pseudo Genes (internal stop)      :: 9 of 47
            Pseudo Genes (multiple problems)  :: 11 of 47
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..17916
                     /organism="Streptococcus pyogenes"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN08555799-rid19682583.denovo.031"
                     /strain="MGAS11113"
                     /serotype="Emm28"
                     /isolation_source="normally sterile site"
                     /host="Homo sapiens"
                     /db_xref="taxon:1314"
                     /country="Finland"
                     /collection_date="2002"
                     /collected_by="National Public Health Laboratory"
     gene            33..1355
                     /gene="ska"
                     /locus_tag="VC122_001113"
     CDS             33..1355
                     /gene="ska"
                     /locus_tag="VC122_001113"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_023611186.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="streptokinase"
                     /protein_id="HEP5641041.1"
                     /translation="MKNYLSFGMFALLFALTFGTVKPVQAIAGPEWLLDRPSVNNSQL
                     VVSVAGTVEGTNQEISLKFFEIDLTSQPAHGGKTEQGLSPKSKPFATNSSAMPHKLEK
                     ADLLKAIQEQLIANVHSNDGYFEVIDFASDATITDRNGKVYFADRDDSVTLPTQPVQE
                     FLLSGHVRVRPYQPKAVHNSAERVNVNYEVSFVSETGNLDFTPSLKEQYHLTTLAVGD
                     SLSSQELAAIAQFILSKKHPDYIITKRDSSIVTHDNDIFRTILPMDQEFTYHIKDREQ
                     AYGINKKSGQEEKMNNTDLISEKYYILKKGEKPYDPFDRSHLKLFTINYVDVNTNKLL
                     KSEQLLTASEHNLDFRDLYDPRDKAKLLYNNLDAFGIMDYTLTGKVEDNHNDTNRIIT
                     VYMGKRPEGENASYHLAYDKDRYTEEEREVYSYLRYTGTPIPDNPNDK"
     gene            complement(1453..1896)
                     /locus_tag="VC122_001114"
     CDS             complement(1453..1896)
                     /locus_tag="VC122_001114"
                     /EC_number="3.1.1.96"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002993699.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-tyrosyl-tRNA(Tyr) deacylase"
                     /protein_id="HEP5641042.1"
                     /translation="MKLVLQRVKEASVSIDGKIAGAINQGLLLLVGVGPDDNAEDLAY
                     AVRKIVNMRIFSDADGKMNQSIQDIKGSILSVSQFTLYADTKKGNRPAFTGAAKPDLA
                     SQLYDSFNEQLAEFVPVERGVFGADMQVSLINDGPVTIILDTKCH"
     gene            complement(1911..4130)
                     /locus_tag="VC122_001115"
     CDS             complement(1911..4130)
                     /locus_tag="VC122_001115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019316080.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional (p)ppGpp
                     synthetase/guanosine-3',5'-bis(diphosphate)
                     3'-pyrophosphohydrolase"
                     /protein_id="HEP5641043.1"
                     /translation="MAKIMNVTGEEVIALAATYMTKTDVAFVAKALAYATAAHFYQVR
                     KSGEPYIVHPIQVAGILADLHLDAVTVACGFLHDVVEDTDITLDEIEADFGQDARDIV
                     DGVTKLGKVEYKSHEEQLAENHRKMLMAMSKDIRVILVKLADRLHNMRTLKHLRKDKQ
                     ERISRETMEIYAPLAHRLGISRIKWELEDLAFRYLNETEFYKISHMMKEKRREREALV
                     EAIVSKVKTYTTQQGLFGDVYGRPKHIYSIYRKMRDKKKRFDQIFDLIAIRCVMETQS
                     DVYAMVGYIHELWRPMPGRFKDYIAAPKANGYQSIHTTVYGPKGPIEIQIRTKDMHQV
                     AEYGVAAHWAYKKGVRGKVNQAEQAVGMNWIKELVELQDASNGDAVDFVDSVKEDIFS
                     ERIYVFTPTGAVQELPKESGPIDFAYAIHTQIGEKATGAKVNGRMVPLTAKLKTGDVV
                     EIITNANSFGPSRDWVKLVKTNKARNKIRQFFKNQDKELSVNKGRDLLVSYFQEQGYV
                     ANKYLDKKRIEAILPKVSVKSEESLYAAVGFGDISPISVFNKLTEKERREEERAKAKA
                     EAEELVKGGEVKHENKDVLKVRSENGVIIQGASGLLMRIAKCCNPVPGDPIDGYITKG
                     RGIAIHRSDCHNIKSQDGYQERLIEVEWDLDNSSKDYQAEIDIYGLNRSGLLNDVLQI
                     LSNSTKSISTVNAQPTKDMKFANIHVSFGIPNLMHLTTVVEKIKAVPDVYSVKRTNG"
     gene            complement(4382..5407)
                     /locus_tag="VC122_001116"
     CDS             complement(4382..5407)
                     /locus_tag="VC122_001116"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010922693.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LPXTG cell wall anchor domain-containing
                     protein"
                     /protein_id="HEP5641044.1"
                     /translation="MLTSKHHNLNKLVWRYGLTSAAAVLLAFGGGASSVKAEVSTAAM
                     TKEERQQEIEKIKEDIKKEYPKASEEKFWESSFWGRRYFNEQEYLKSLQSFTEQRLQD
                     IVNLIEKGATKGDKGDAGPKGERGPAGPQGPVGPKGEAGKVGAQGPKGDPGAPGKDGA
                     KGEKGDKGDTGERGEKGDIGATGAQGPQGEKGETGAQGPAGPQGEAGKPGEQGPAGPQ
                     GEAGKPGEKAPEKSPEGEAGQPGEKAPEKSPEVTPTPEMPEQPGEKAPEKSKEVTPAP
                     EKPADKEANQTPERRNGNMAKTPVANNHRRLPATGEQANPFFTAAAVAVMTTAGVLAV
                     TKRKENN"
     gene            5789..6271
                     /gene="nrdI"
                     /locus_tag="VC122_001117"
     CDS             5789..6271
                     /gene="nrdI"
                     /locus_tag="VC122_001117"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012561021.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class Ib ribonucleoside-diphosphate reductase
                     assembly flavoprotein NrdI"
                     /protein_id="HEP5641045.1"
                     /translation="MPQITLVFISLSGNTLSFVKRLSLYLADNYDYHVKQINIKDLKH
                     ETFPVKEEFVAILPTYLEGGNGVDSGEVEILTTPLGEFIAAHGNAQRCLGIIGSGNKN
                     FNHQYCLTAKQYAKRFGFPLLGDFELRGTPDDISRLAQVIMEASSRHSSNDTQTLPNS
                     "
     gene            complement(6665..7483)
                     /locus_tag="VC122_001118"
     CDS             complement(6665..7483)
                     /locus_tag="VC122_001118"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014635731.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease/exonuclease/phosphatase family
                     protein"
                     /protein_id="HEP5641046.1"
                     /translation="MTKVLTLNTHSWMQANTLKKLVALAEHILAEKYDIICLQEINQL
                     IESELATDLPRYQALSGTPNIHKDHFALLLIHYLQKRGQHYYWSWAYNHIGYDIYQEG
                     VAILSKQPIHVSDILVSAMADETDYHTRRSLIAKTTLDGKEVAVVNVHLSWFDKGFLG
                     EWEKLEKELLTLNCPLLLMGDFNNPTDQDGYQVMMGSPLDLQDSHKVADHVFGDHSIV
                     ADIDGWQGNKEALKVDHVFTSKDFIIRSSKITFEGGDAPVVSDHYGLEVTLGWR"
     gene            complement(7566..9752)
                     /locus_tag="VC122_001119"
     CDS             complement(7566..9752)
                     /locus_tag="VC122_001119"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014635732.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit EIIC"
                     /protein_id="HEP5641047.1"
                     /translation="MKTSFKQLFRFEFWQKFGKCLMVVIAVMPAAGLMISIGNSIPMI
                     NHESAFLASLGNIIAQIGWAVIVNLHLLFALAIGGSWAKERAGGALASGLAFVLINRI
                     TGAFYGVSSAMLADPEAKITSLLGTQMIVKDYFTSVLESPALNTGVFVGIIAGFVGAT
                     AYNKYYNYRKLPEVLTFFNGKRFVPFVVILRSIFVALILVVVWPVIQSGINSFGMWIA
                     SSQDSAPILAPFLYGTLERLLLPFGLHHMLTIPMNYTALGGTYEVMTGAAAGTKVFGQ
                     DPLWLAWVTDLVHLKGSDASAYSHLMDSVTPARFKVGQMIGATGTLMGVALAMYRNVD
                     ADKKHTYKMMFISAAAAVFLTGVTEPLEYLFMFAAMPLYIVYALVQGASFAMADLVNL
                     RVHSFGNIELLTRTPMALKAGLGMDVINFVWVSILFAVIMYFIADMMIKKMHLATAGR
                     LGNYDADILGDHNTQATPTQVADSNSQVVQIINLLGGAGNIDDVDACMTRLRVTVKDP
                     AKIGAEDDWKKAGAMGLIQKGNGVQAVYGPKADILKSDIQDLLDSGAVIPEVNMSQLT
                     SKPTPAKDFKHVTEEVLSVADGMVLPITGVKDQVFAAKMMGDGFAVEPTHGNIYAPVA
                     GLVTSVFPTKHAFGLLTDNGLEVLVHVGLDTVALNGVPFSVKVSEGQRVHAGDLLVVA
                     DLAAIKSAERETTIVVAFTNTTEIQDVTLTSLGAQPAKTKVATVEL"
     gene            complement(10109..10858)
                     /locus_tag="VC122_001120"
     CDS             complement(10109..10858)
                     /locus_tag="VC122_001120"
                     /EC_number="2.1.1.193"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014635733.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (uracil(1498)-N(3))-methyltransferase"
                     /protein_id="HEP5641048.1"
                     /translation="MQQYFIKGKAEKEVTITDKDTIKHMFQVMRLADKAEVVLVFDDG
                     VKYLAKVTNSMAHELEIIEALPDQVELPVKVTIASGFPKGDKLDTIAQKVTELGASAL
                     WGYPADWSVVKWDGKKLAKKEDKLAKIVLGAAEQSKRNRVPEVHLFEHKAEFLKSLSG
                     FDHIFVAYEETAKAGQLATLAREVKEVKPGAKILFIFGPEGGISPTEITQFEAAGAIK
                     VGLGPRIMRAETAPLYALSALSYALELQASK"
     gene            complement(10858..11811)
                     /locus_tag="VC122_001121"
     CDS             complement(10858..11811)
                     /locus_tag="VC122_001121"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011055040.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L11 methyltransferase"
                     /protein_id="HEP5641049.1"
                     /translation="METWQEVTVHVHRDAQEAVSHVLIETGSQGVAIADSADYIGQKD
                     RFGELYPDVEQSDMIAIIAYYPSSTNLADVIATINEQLAELASFGLQVGQVTVDSQEL
                     AEEDWADNWKKYYEPARITHDLTIVPSWTDYDASAGEKVIKLDPGMAFGTGTHPTTKM
                     SLFALEQILRGGETVIDVGTGSGVLSIASSLLGAKTIYAYDLDDVAVRVAQENIDLNQ
                     GTDNIHVAAGDLLKGVSQEADVIVANILADILVLLTDDAYRLIKKEGYLILSGIISEK
                     LDMVLEAAFSAGFFLETHMVQGEWNALVFKKTDDISGVIGG"
     gene            complement(11881..12351)
                     /locus_tag="VC122_001122"
     CDS             complement(11881..12351)
                     /locus_tag="VC122_001122"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011018260.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3013 family protein"
                     /protein_id="HEP5641050.1"
                     /translation="MAKYGFLSVLEEEMDKHFQYDYAMDWDKKNHAVEVTFVLEAQNK
                     EAIKTIDDSGEVTQDDIVFEDYVLFYNPAKSQFDAADYLVTIPFDAKKGFSREFLAYF
                     AQFLNDVAIEGHSDLMDFLADDSKVDFGLEWNAQAFEEGQQGLEEAASYAYPRY"
     gene            12552..14309
                     /gene="pabB"
                     /locus_tag="VC122_001123"
     CDS             12552..14309
                     /gene="pabB"
                     /locus_tag="VC122_001123"
                     /EC_number="2.6.1.85"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014635737.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminodeoxychorismate synthase component I"
                     /protein_id="HEP5641051.1"
                     /translation="MHRKTIIDFKELGQRYLFDEPLVELVAKSLDQVGPVIENVQHYQ
                     QLGYYVVGYLSYEAAAFFDNALQTHNDRLGNEYLAYFTVHKTCQKKDLPLDYDGITIP
                     NQWVSATQKEAYQKAIETIHREMQQGNTYQVNYTLQLTQELNAADSLAIYNKLVVEQA
                     AGYNAYIAHDDFAVISASPELFFKQEGNRLTTRPMKGTTKRGVNSWLDQQEHDWLQAD
                     GKNRSENMMIVDLLRNDMGKICQTGSVCVDRLCEVERYSTVWQMTSTIVGDVKADCDL
                     IDILTALFPCGSITGAPKVSTMAIITSLEPKPRGIYCGSIGICLPDGRRFFNVPIRTI
                     QLSHNQATYGVGGGITWQSKWEDEYEEVHQKTAFLYRHKQIFDLKTTAKVKHKKIAFL
                     EQHLNRLKEAATYFAYPYDEKALQKQLSTYLENKDNAAYRLMIRLSKDGKISLSDQPL
                     EPLSADFLTAQLSLQKKDVTASPFTYFKTSYRPHIEQKSYEQLFYNQAGQLLETSIGN
                     LFVQLGRTLYTPPVVVGILPGLFRQELLATGQAQEKEVTLADLKEASAIFGGNAVRGL
                     YPLNLKLTHLDALLAKSQA"
     gene            14342..14908
                     /locus_tag="VC122_001124"
     CDS             14342..14908
                     /locus_tag="VC122_001124"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011889181.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminodeoxychorismate/anthranilate synthase
                     component II"
                     /protein_id="HEP5641052.1"
                     /translation="MILLIDNYDSFTYNLAQYLSEFDETIVLYNQDPNLYDMAKKANA
                     LVLSPGPGWPKEANQMPKLIQDFYQTKPILGVCLGHQAIAETLGGNLRLAKRVMHGRQ
                     STIETQGPASLFRSLPQEITVMRYHSIVVDQLPKGFSVTARDCDDQEIMAFEHHTLPL
                     FGLQFHPESIGTPDGMTMIANFIAAIPR"
     gene            14941..16209
                     /locus_tag="VC122_001125"
     CDS             14941..16209
                     /locus_tag="VC122_001125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000774759.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication-associated recombination protein A"
                     /protein_id="HEP5641053.1"
                     /translation="MPDHLALRMRPKTISEVIGQKHLVGEGKIIRRMVEANRLSSMIL
                     YGPPGIGKTSIASAIAGTTRYAFRTFNATIDSKKRLQEIAEEAKFSGGLVLLLDEIHR
                     LDKTKQDFLLPLLENGTIIMIGATTENPFFSVTPAIRSRVQIFELEPLSNEDIKTAIQ
                     LAISDKERGFPFLVTIDDEALDFIVTATNGDLRSAYNSLDLAVMSTSPNEDSSRHISL
                     ETMENSLQRSYITMDKNGDGHYDVLSALQKSIRGSDVNASLHYAARLVEAGDLPSLAR
                     RLTIIAYEDIGLANPDAQVHTVTALDAAQRIGFPEARILIANVVIDLALSPKSNSAYL
                     AMDAALADLRTSGNLPIPRHLRDGHYAGSKDLGNAKDYLYPHAYPEKWVKQQYLPDKL
                     VGHHYFEANETGKYERALGSNKERIDKLSD"
     gene            16298..16494
                     /gene="ssrS"
                     /locus_tag="VC122_001126"
     ncRNA           16298..16494
                     /ncRNA_class="other"
                     /gene="ssrS"
                     /locus_tag="VC122_001126"
                     /product="6S RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00013"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00013"
     gene            16495..16567
                     /locus_tag="VC122_001127"
     tRNA            16495..16567
                     /locus_tag="VC122_001127"
                     /product="tRNA-Lys"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:16528..16530,aa:Lys,seq:ctt)
     gene            16708..17148
                     /locus_tag="VC122_001128"
     CDS             16708..17148
                     /locus_tag="VC122_001128"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012561036.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HEP5641054.1"
                     /translation="MKIIEYKGNYRDDLIFMVLEAKNALRRIPSINPDLLTIETSYLE
                     KGDCFWLAVNEESRVIGSIAYSSIPKTNEVFLHRLFIKPSLKHQGIGSQLLTFAEDYL
                     RKIGKSHVRIHLGQPKEQWFESYRFYPKHGYQFYDDTHLIKKLI"
     gene            17203..17709
                     /locus_tag="VC122_001129"
     CDS             17203..17709
                     /locus_tag="VC122_001129"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011185008.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topology modulation protein"
                     /protein_id="HEP5641055.1"
                     /translation="MKIAIIGHSGSGKSTLARFLGQHYHCEVVHLDQLHFSSNWQERS
                     DHDMIADLSTCLLKQDWIIEGNYANCLYEERMSEANHIIYVNFSRFHCVYRAFKRYLN
                     YRGKTRPDMADNCQEKFDVAFVKWILLDGRSRNQLKKYQSVIQKYPHKTIVLTNQKQL
                     NHYMNTIN"
BASE COUNT         4997 a         4173 c         3517 g         5229 t
ORIGIN      
        1 ggggattgaa acttaacttt taggaggttt ctatgaaaaa ttacttatct tttgggatgt
       61 ttgcactgct gtttgcatta acatttggaa cagtcaagcc tgtccaagct attgctggac
      121 ctgaatggct gctagaccgt ccatctgtca acaacagcca attagttgtt agcgttgctg
      181 gtactgttga ggggacgaat caagaaatta gccttaaatt ttttgaaatt gacctaacat
      241 cacaacctgc tcatggagga aagacagagc aaggcttaag tccaaaatca aaaccatttg
      301 ctacaaatag tagcgcaatg ccacataaac ttgaaaaagc tgacttatta aaggctattc
      361 aagaacaatt gatcgctaac gttcacagta acgacggcta ctttgaggtc attgattttg
      421 caagcgatgc aaccattact gatcgaaacg gcaaggtcta ctttgctgac cgagatgatt
      481 cggtaacctt gccgacccaa cctgtccaag aatttttgct aagcgggcat gtgcgcgtta
      541 gaccgtatca acctaaagcc gttcacaact cagctgaacg cgttaacgtc aactatgaag
      601 tgagctttgt ctccgaaaca ggaaatttag actttacgcc atcgttaaaa gagcaatacc
      661 atttgaccac actggcagtt ggtgactctc tttcatcaca agagttagca gcaattgccc
      721 aatttatcct atcaaaaaaa catccagatt atatcattac aaaacgtgac tcctcaatcg
      781 tcactcatga caatgacatt ttccgtacga ttttaccaat ggatcaagag tttacttacc
      841 atatcaaaga ccgggaacaa gcttatggga tcaataaaaa atctggtcag gaagaaaaaa
      901 tgaacaacac tgaccttatc tctgagaaat attacatcct taaaaaaggg gaaaagccgt
      961 atgatccctt tgatcgcagt cacttgaaac tgttcaccat caattacgtt gatgtcaaca
     1021 ccaacaaatt gctaaaaagc gagcagctct taacagctag cgaacataac ttagacttca
     1081 gagatttata cgatcctcgt gataaggcta aactactcta caacaatctc gatgcttttg
     1141 gtattatgga ctatacctta actggaaaag tagaggataa tcacaatgac accaaccgta
     1201 tcataaccgt ttatatgggc aagcgacccg aaggagagaa tgctagctat catttagctt
     1261 atgataaaga tcgttatacc gaagaagaac gagaagttta cagctacctg cgttatacag
     1321 ggacacctat acctgataac cctaacgaca aataaccacg atcttctaaa acgatgagat
     1381 taactgacaa aaaaagcaag caacacgcta tcaacagttg cttgcttttt tctaacctct
     1441 tagttgtaga gactagtgac atttcgtgtc taaaataatc gtaactggtc catcattgat
     1501 gagactaacc tgcatatctg ccccaaaaac gccacgctca actggcacaa actctgccaa
     1561 ttgttcatta aagctatcat acaactggct agccaaatct ggcttagcag caccagtaaa
     1621 ggcaggacgg tttccctttt tggtgtcagc ataaagggta aattgcgaca cagataagat
     1681 actgcccttg atgtcttgga tagactgatt catcttgcca tcagcatctg aaaaaatgcg
     1741 catgttgact atttttcgca cagcgtaagc caaatcttct gcattatcat ctggtcccac
     1801 acctactaac aacaacaacc cttggttaat agcgccagca attttcccat caatagaaac
     1861 agaggcctct ttgacccgtt gcaagacaag tttcatggtt ttatcctttc ttagccattg
     1921 gtccgcttca cgctataaac atctggaact gccttgattt tttcgacaac agtggtcaga
     1981 tgcatcagat ttggaatgcc aaagctcacg tgaatattag caaacttcat gtccttggtc
     2041 ggctgagcat tgactgtcga tatgctcttg gttgagtttg ataaaatttg gagcacatca
     2101 ttgagcaggc cactacgatt gagcccatag atatcaattt cagcctgata atctttactc
     2161 gaattgtcca aatcccactc gacctcaatc aagcgttctt ggtagccatc ttgactctta
     2221 atgttatgac agtccgatct gtgaatcgca atgccacgcc ctttggtaat atagccgtca
     2281 ataggatcac caggtacagg attacaacac ttggcaatcc gcatcaagag gcctgatgct
     2341 ccttggataa tgaccccatt ttcactgcga accttgagca catctttgtt ttcgtgtttg
     2401 acctcaccgc ccttaaccaa ttcttcagct tctgctttag ccttggccct ttcttcttca
     2461 cggcgctctt tttcggttaa cttgttaaag acactgatag gactaatgtc accaaaccca
     2521 acggctgcat agagtgattc ttcgctcttc acactgactt ttggaaggat cgcttcaatg
     2581 cgttttttgt caaggtattt attggcaaca tagccctgct cttgaaaata agacaccaac
     2641 aaatcacggc ctttattcac tgacaattcc ttgtcttgat ttttaaagaa ctgacgaatt
     2701 ttgttgcgag ccttattggt tttgaccagt tttacccagt ctcgactagg gccaaaggaa
     2761 ttggcattgg tgatgatttc aaccacatct cctgttttta acttggcagt gagaggaacc
     2821 atacgtccat tgactttggc acctgttgct ttttcaccga tttgcgtatg gatcgcataa
     2881 gcaaaatcaa taggacctga ttcttttggc aactcctgaa cggcccctgt cggtgtaaag
     2941 acataaatcc gttcagaaaa aatgtcttct ttgaccgaat ccacaaagtc cactgcatcg
     3001 ccatttgagg catcttgcaa ttctaccagc tctttgatcc agttcatgcc aacggcttgc
     3061 tcagcctgat tgaccttacc acgcacgcct tttttataag cccagtgagc agcaaccccg
     3121 tactcagcca cttgatgcat gtccttagtt ctgatttgaa tctcaatagg tccttttggt
     3181 ccatacacag tggtatgaat cgactggtag ccattagctt taggagctgc aatataatcc
     3241 ttgaagcggc ctggcatggg acgccaaagc tcatgaatat agccaaccat agcataaaca
     3301 tcgctctgcg tttccatgac acaacggata gcaatcaaat caaaaatctg atcgaaacgt
     3361 ttctttttgt cccgcatttt ccgataaatc gaataaatgt gttttggtcg gccatacaca
     3421 tctccaaaca acccttgttg tgtcgtatag gttttgacct tactgacaat agcctctacc
     3481 aaagcttcac gctcgcgacg tttttctttc atcatatggg aaattttgta aaattcggtt
     3541 tcattgaggt aacgaaaagc caaatcttct agttcccatt tgatgcgact aatccccaaa
     3601 cgatgcgcca agggggcata gatttccatg gtttcgcgcg aaatgcgctc ttgtttgtcc
     3661 ttgcgcaaat gtttgagggt gcgcatatta tgcaggcggt cagccaattt caccaaaatc
     3721 acgcgaatat ctttagacat agccatcagc attttgcgat ggttttcggc gagttgctcc
     3781 tcatgagatt tgtactcaac tttacctaac ttggtgacac catcaacgat atcacgagca
     3841 tcttggccaa agtctgcttc gatctcatct aaggtaatat ccgtatcttc tacgacatca
     3901 tgtaaaaagc cacaggcaac tgtcacagca tccagatgca aatcagccag aatccccgcc
     3961 acctgaatcg gatggacgat atagggttcg cctgactttc tcacttggta gaaatgggcc
     4021 gctgttgcat atgctaaagc ctttgccaca aaagccacat cagtcttggt catataggtg
     4081 gccgctaagg caatgacttc ttctcctgtt acgttcatta tttttgccat ttcgttcctc
     4141 atctagcatt tcttttccct attttaacac tttacacaca aaagcaaaag gcagaatctg
     4201 ctaagaaagc gcttagtgtg accttttctt agaaaacggt tagtgagctg cttttgatat
     4261 cttttgggat ctctcaggcg tgagcatgat accaacggcc agctcccgac caaaaataga
     4321 gagaaccaag gggctctctc tattatgttt gcttatcagt aaggtctctc aattcagagg
     4381 attagttgtt ttctttgcgt tttgtaacgg ctaggacacc agctgttgtc atcactgcta
     4441 ctgctgctgc tgtaaagaat gggttggctt gctcaccagt tgctggtaga cgtctgtggt
     4501 tgttggctac aggtgtctta gccatattgc cattgcggcg ttctggcgtt tggttagctt
     4561 ctttgtcagc aggtttttct ggagctggag ttacctcttt gctcttttct ggagcttttt
     4621 cacctggttg ctctggcatt tctggcgttg gtgtcacttc tgggctcttt tctggagctt
     4681 tttcgcctgg ttggcctgct tcgccttctg ggctcttttc tggagctttt tcacctggtt
     4741 tgcctgcttc accttgtggg cctgctggac cttgctcacc tggtttgcct gcttcacctt
     4801 gtgggcctgc tgggccttgt gcgccagttt cacctttttc accttgtggg ccttgtgcgc
     4861 cagttgctcc gatgtcacct ttttcaccgc gctcaccggt atcacctttg tcgccttttt
     4921 cacctttggc gccgtcttta cctggggcac caggatcacc tttaggacct tgggcaccaa
     4981 ccttacctgc ttcaccttta ggtcctaccg gaccttgtgg gcctgctggg ccgcgctcgc
     5041 ctttaggacc agcatcgcct ttgtcacctt ttgttgcacc tttttcaatc agatttacaa
     5101 tatcttggag tctttgctca gtaaaacttt gtaaagattt tagatactct tgttcattaa
     5161 agtaccttct tccccaaaag ctactctccc aaaatttttc ctcagacgct tttggatatt
     5221 ctttcttaat gtcttcttta attttctcaa tttcttgttg tctctcctcc ttcgtcatag
     5281 ccgcagtaga aacctcagcc ttaacgctgc ttgccccgcc tccaaaggct agaaggacgg
     5341 cagcggctga ggttagcccg tagcgccaga ctagtttgtt gagattgtgg tgttttgatg
     5401 tcaacatatg ttgttctctc tttctttatt agatggtgtg ttttagataa aaataacatg
     5461 gtagctagac ctgattattt ataatagtat tataatagag tgtgaaaacg attgtaaagt
     5521 tctcttttgt tgttcatctt ttagattttg actgatcact cttttgtgga gatcagaggg
     5581 ctactttggc acttgcctgc ctcgttttta agtcgagggc atccggatcc tttctttaag
     5641 gtcactcttt tttgaaatgt tacttttttt gaccgcttgt accttcttgt ctggttggat
     5701 atcgctaggt ttcttcttta cttgtttgtt ggtcttgatt gtggtaagct tatgaagaaa
     5761 ccttgtagga taaaaaaagg agacacttat gcctcagatt actcttgttt ttattagcct
     5821 cagtggcaac acccttagct ttgtcaagcg gctatccctt tatttagcag acaattacga
     5881 ttaccatgtc aaacagatta acattaaaga tttgaagcac gagacttttc ccgtcaagga
     5941 agagtttgtg gcgattctac ccacttatct agaaggcgga aatggcgttg actctggtga
     6001 ggtggagatc ctcaccactc ctttaggaga gtttattgcc gcccatggca acgcccagcg
     6061 ttgtcttggg attattggtt caggcaacaa aaactttaac catcagtatt gcctgacagc
     6121 caaacagtat gccaaacgct ttggctttcc actgctaggt gattttgagc tgcgagggac
     6181 tcctgatgac atttcgcggt tagcccaggt catcatggag gccagctcca gacactcgtc
     6241 taacgacaca cagacactgc ctaacagcta gaccgtctat agatgatcat agctgtttta
     6301 aaaaagagca cttttaggac accaactagg gaggtctctt accctacgcc ttgcgatttc
     6361 tcagcctttg aaaagagcga tcacagtgct tcttggggct tgggatcact ccaagcgctc
     6421 ctagaaatac ccctatgcag actttagcaa ggcaactgaa cgcttggcgg tactttctgt
     6481 gaagctcaga tggttggtgg caaaaaagcc ctggtttttt agggttactg acatgactaa
     6541 agaggtctaa ccagctctac cttgagctcg ttagacctct ttgtgttctc ctaatccccc
     6601 tagacgatcg cagtttctta tgtgtcttgg cttctcctat gtggtgtgcc tgttagcaca
     6661 tgctctaacg ccagcctaat gtcacttcta aaccgtagtg gtcactgacg actggcgcat
     6721 ctccgccttc aaaggtaatc ttagaagatc ggatgatgaa gtccttgctg gtaaagacat
     6781 ggtccacctt gagggcttct ttgttacctt gccagccatc aatatcagcc acgatgctat
     6841 gatcaccaaa cacatggtca gcaaccttat ggctatcttg taggtctagg gggctaccca
     6901 tcatcacttg ataaccgtct tgatccgttg gattgttaaa atctcccata agtagcaagg
     6961 gacagtttag tgtgagcagt tctttttcta atttctccca ctcccctaaa aaccctttgt
     7021 caaaccaaga taggtgcaca ttgacaacag cgacttcttt gccatctaac gtggttttgg
     7081 ctatgagtga gcgtctggtg tggtagtctg tttcatcagc catcgctgac acgagaatat
     7141 ctgaaacatg aataggttgt tttgataaga ttgccacgcc ttcctggtaa atgtcatagc
     7201 caatatggtt ataagcccaa gaccaataat agtgctgccc tcgtttttgt aaataatgga
     7261 tcaagagcag ggcaaaatgg tctttatgaa tgtttggtgt ccctgaaaga gcttgatatc
     7321 ttggtaaatc tgtggccagc tcgctctcga ttaactgatt gatttcttgc aagcaaatga
     7381 tatcgtattt ttcagctaaa atatgctcag ccaaagcaac taatttttta agagtgttgg
     7441 cttgcatcca cgaatgggta ttcagtgtta ataccttggt cattaatcct cctttataag
     7501 acaagaagag ccatagggcc taaaccagct atggctatta cctatcacta tctcgtgatg
     7561 ttttgttaaa gttctacggt agctacttta gtcttagcag gctgagctcc taaagatgtc
     7621 aaggtcacat cttggatctc tgttgtgttg gtaaaagcaa caacaatggt tgtttcacgc
     7681 tctgctgatt taatagcagc aagatctgct acgactaata ggtcacctgc atgaacccgt
     7741 tgtccttctg agactttgac tgaaaaaggc acaccattta aggcgacggt atcaagtccc
     7801 acgtgcacca gcacttcaag accattatct gtcagtaaac caaaggcatg cttggtcgga
     7861 aagacactgg tcacaaggcc agctactggt gcatagatat taccatgggt tggttcaacc
     7921 gcaaacccat ctcccatcat cttagccgca aacacctggt ctttcacacc tgtgattggg
     7981 agaaccatcc cgtctgccac tgatagcact tcttctgtca cgtgtttaaa atcttttgcg
     8041 ggagtcggtt tgctagtgag ctgtgacatg ttgacttctg gaataacagc ccctgagtcc
     8101 agcaaatctt gaatatctga tttcaaaata tctgctttag gcccatagac cgcttgaacg
     8161 ccgttgcctt tttggatcaa gcccatagcg ccagcttttt tccaatcgtc ctcagcgcca
     8221 atcttagcgg ggtctttgac cgtcactcgt aagcgcgtca tacaagcatc aacatcatca
     8281 atattgcctg ccccaccaag gagattaatg atttgtacga cttgagagtt gctatctgct
     8341 acttgggtag gtgttgcttg agtgttatgg tcacccagta tatcggcatc gtagttgcct
     8401 aaacggccag ctgtcgcgag atgcattttc ttgatcatca tatccgcaat aaagtacatg
     8461 ataactgcaa agaggataga aacccaaaca aagttaatca catccatgcc tagacctgct
     8521 ttaagggcca taggggtacg ggttagtagc tcaatgttcc caaatgagtg aacacggaga
     8581 ttcacaaggt ctgccatggc aaatgaagct ccttgaacaa gggcatagac gatgtaaagt
     8641 ggcattgctg caaacataaa taggtactca agtggctcag ttacccctgt caaaaagacc
     8701 gctgccgctg ctgagatgaa catcattttg tatgtatgtt ttttatcggc atccacatta
     8761 cggtacatgg ctaaggcaac ccccatcaat gttccggtag ctccaatcat ttgtcctacc
     8821 ttaaagcgag ctggagtcac actatccatt aagtgactat aggctgaagc gtctgatcct
     8881 ttaaggtgaa caaggtcagt tacccaagca agccacaaag ggtcttgacc aaatactttt
     8941 gtccccgctg ctgcacctgt catgacctca taagttccac caagagctgt atagttcatt
     9001 ggaatcgtca acatgtggtg aagaccaaat ggcaacaaga gacgttccaa ggtaccatat
     9061 aggaatgggg ctaggattgg agctgaatct tgtgaagagg caatccacat cccaaaactg
     9121 ttaatcccag actggatcac cggccaaaca acaaccaaaa taagggctac aaaaatagaa
     9181 cgtaaaataa cgacaaacgg tacaaaacgc ttcccattaa agaaggtcaa aacttcagga
     9241 agtttacggt aattatagta tttgttatag gccgttgctc ctacgaaccc tgcaataatc
     9301 ccaacaaaaa cccctgtgtt taaagctgga gactccaaga cactggtgaa ataatcttta
     9361 acgatcattt gagtgccaag gaggcttgtg atttttgctt ctggatcagc tagcatagca
     9421 ctagacacgc cataaaaagc acctgttatc cgattgatca agacaaaagc aagccctgag
     9481 gcaagggccc ctccagctct ttctttagcc caactaccac caatggccaa ggcaaatagc
     9541 aaatgaaggt taacaataac agcccaacca atttgagcga taatgttgcc tagagatgcc
     9601 aaaaatgctg attcgtggtt aatcattgga atagagtttc cgatactgat cattaaacca
     9661 gccgctggca tgacagcaat aacgaccata agacatttcc caaatttttg ccaaaattcg
     9721 aaacgaaaca gttgcttgaa tgatgttttc attctatctc tcctttatta tcccctttta
     9781 gccatagctg cccttaaggc gcttttgtga cacaatgata tagactgcaa ctcagtgatg
     9841 caatcgtttg cattgatctg atacctttat tatactagcc tcgcaacaat ttgtaaagcg
     9901 ttttcttaga cagatgttag tcgtttttct tcattaaatg gtagtgtaac ttaccaaaaa
     9961 gataacaaga gcccatttcc ttttttggtg atgagaccat caacgctctt cgaaaaaaca
    10021 agaagcaata aaaaaaggcc gttgacacaa tcgtgaactg cctgttcgtt tctttagcct
    10081 cttctttcta aagtaagcag cagcttactt attttgatgc ctgcaattcc aaagcataac
    10141 taagagcact taaggcatat agcggcgctg tctctgctcg cataatgcga gggcctaagc
    10201 ccaccttgat ggcacctgct gcctcaaact gagtgatttc agttggggaa attccccctt
    10261 ctggtccaaa gataaagagg atttttgccc ctggcttaac ttcttttact tcacgcgcca
    10321 aggtcgctag ctgacctgcc ttagctgttt cttcgtatgc aacgaaaata tgatcaaagc
    10381 cagaaaggct ttttagaaac tctgctttgt gctcaaaaag gtgaacttca ggcaccctat
    10441 ttcgtttgct ctgctcagcc gctcctaata caatcttagc caatttgtct tcttttttag
    10501 ctaatttttt gccatcccat ttgaccactg accagtctgc tgggtagccc catagcgcac
    10561 tggctcccaa ttcagtcact ttctgtgcaa tcgtgtctaa cttatcgcct ttaggaaatc
    10621 cagaagcaat agtcacctta acaggcaatt ccacctgatc tggcaaagcc tctataatct
    10681 ctaactcatg agccatacta ttggtcacct tggctaaata cttcacccca tcatcaaaga
    10741 ccaagacaac ttcagccttg tcagccaagc gcatcacttg aaacatgtgc ttgatggtat
    10801 ctttatcggt aattgttacc tctttttctg ctttgccttt aataaaatac tgttgcatta
    10861 gccgccaatc actcctgaga tatcatctgt ttttttaaag acaagggcgt tccactcacc
    10921 ttgaaccata tgcgtctcaa ggaagaatcc tgcgctaaag gcagcttcca gaaccatatc
    10981 caatttctca gaaatgatgc ctgataagat aaggtaacct tcctttttga tcaaacggta
    11041 agcatcatca gtcaataaga ctaagatatc cgccaagata ttggctacaa tcacatccgc
    11101 ttcttggcta acccctttta aaaggtcacc agcagccaca tgaatgttgt ctgttccttg
    11161 atttaaatca atgttttctt gagccactcg aacagctaca tcatctaagt cataagcgta
    11221 gatggtttta gcacctagta aggaactggc aatcgagagg acacctgatc cggtccctac
    11281 atcgataact gtctcaccac cacgaaggat ttgttctaga gcaaaaaggc tcatcttagt
    11341 tgtgggatgt gtccctgtcc caaatgccat tccaggatct aatttaataa ccttttcccc
    11401 agcagaagcg tcataatccg tccaactagg gacaatagtt aaatcatgcg tgatgcgagc
    11461 tggttcatag tattttttcc aattgtctgc ccagtcttcc tcagctaatt cttgactatc
    11521 cacagtaacc tgaccaacct gtaacccaaa actagctaac tccgctaact gttcattaat
    11581 ggtcgcaata acatccgcca aattagtgct acttgggtaa taggctatga tagctatcat
    11641 gtcagactgc tccacatctg ggtataactc cccaaagcgg tccttttgcc caatatagtc
    11701 tgcgctatcc gcgatagcga ccccttggct gcctgtctca atcaacacat gtgaaacagc
    11761 ctcttgcgca tcacgatgca catgaaccgt cacttcttgc cacgtttcca taagtatgtc
    11821 ctttctattt tgaccttttg gttattgtcc taatggtgtt acaccatctc gtgtgtcacc
    11881 ttagtacctt gggtaagcgt aacttgctgc ttcttctagt ccttgttgac cttcttcaaa
    11941 agcttgcgca ttccattcca agccgaaatc aaccttgctg tcatctgcta agaaatccat
    12001 caaatcgctg tgcccttcaa tagcaacatc gttgagaaat tgcgcaaagt aagctaagaa
    12061 ttcacgagaa aagccctttt tagcgtcaaa aggaatagta accaaataat ctgctgcatc
    12121 aaattgagac ttggcagggt tgtaaaagag cacatagtcc tcaaagacaa tatcatcttg
    12181 ggtcacttcg ccactgtcgt caatggtttt tatggcctct ttattttgag cttctaacac
    12241 aaaagtcact tcaaccgcat gatttttctt gtcccaatcc atagcataat catactgaaa
    12301 atgcttatcc atttcttctt ctaatactga taaaaagccg tattttgcca tcttttttta
    12361 tacctaatct ttctaactgt tgctgtatag agctttgtaa gacctcatta gtttacttac
    12421 tttgacatgc tgagcgtact gttataactt ttattaactg aattataaca tatttgacta
    12481 atattgacca taagagaagg tcttaatggc tggatcacct ctagcaaccg cttttatgat
    12541 ataatttggg tatgcataga aaaacgatta ttgatttcaa agagctcggg caacgctatc
    12601 tttttgatga gccattagta gaattggtag ccaagtcctt agatcaggtc ggtccagtca
    12661 ttgaaaacgt acaacactac caacagctgg gctattacgt ggtgggctat ctcagttatg
    12721 aagcagctgc tttttttgac aatgccttac aaacccataa tgacagacta ggcaacgagt
    12781 accttgccta ttttacagtg cacaaaacct gtcaaaaaaa agacctacct cttgattatg
    12841 acggcattac tatccctaac caatgggtca gcgcaactca aaaagaagcc tatcaaaaag
    12901 ccattgaaac cattcaccgt gagatgcaac agggcaacac ttatcaggtt aactacaccc
    12961 ttcaattaac tcaggaactc aatgcagctg acagcttagc catttacaat aaactcgtcg
    13021 ttgaacaggc tgctggctac aatgcctata tcgcccatga tgactttgct gttatttcag
    13081 ctagtcctga gttgttcttt aaacaagagg gcaacaggtt aactactagg cctatgaaag
    13141 gcaccaccaa gcgtggggtt aatagctggc tggatcaaca agaacatgac tggctccaag
    13201 ctgacgggaa aaaccgttct gaaaacatga tgattgtgga tttgctccgc aatgacatgg
    13261 gaaaaatttg ccaaacaggc agtgtttgtg tcgataggct ctgtgaggtc gagcgatact
    13321 ctaccgtctg gcaaatgacc tcaaccattg ttggtgacgt caaagcggac tgtgacctga
    13381 tagacattct cacagctctc tttccttgtg gctctatcac gggggctcct aaagtctcca
    13441 ccatggcaat tatcacatct ctggagccaa aaccaagggg gatttattgt ggaagtattg
    13501 gcatttgcct acctgatggt cgccgtttct tcaatgttcc cattagaacc atccagctca
    13561 gtcataatca agctacttat ggtgtcggag gcggtatcac ttggcaaagc aagtgggaag
    13621 acgaatacga ggaagtccat caaaaaacag cttttctcta tcggcacaaa caaatctttg
    13681 acctcaaaac aaccgccaag gtgaaacaca aaaaaatagc cttccttgag caacacctca
    13741 atcgccttaa agaagcagcc acttattttg cttaccctta cgatgagaaa gctttgcaga
    13801 aacaactgtc aacctatttg gaaaataagg ataatgctgc ctaccgcttg atgattcgtt
    13861 tatccaagga tggaaagatt agcctctctg atcaaccctt ggaacctttg tcagccgatt
    13921 ttctgacagc tcaactctct ttacagaaga aggacgtgac agcttcgcct tttacctact
    13981 ttaagaccag ctaccggcct catattgagc aaaaatctta tgaacagcta ttctataacc
    14041 aagcggggca attattggaa acgtccattg gcaatctctt tgttcagctg ggtcggaccc
    14101 tctacacacc accagttgtg gtaggtattt taccaggact ttttcgccaa gagctgctag
    14161 ctactggtca agctcaggaa aaagaggtga cactagctga tttaaaagag gctagcgcta
    14221 tttttggagg aaatgctgtc cgtggcctct atcctctcaa ccttaagctt actcaccttg
    14281 atgccctctt agctaagagt caagcctaaa ctgccactca tcttttgtta ttaaggaacc
    14341 tatgatactc ttaattgata attacgattc atttacctac aacctcgccc aatatttaag
    14401 tgaatttgac gagacgattg tcttgtataa ccaagaccca aacttatatg acatggccaa
    14461 aaaagctaac gctctagtcc tctcacctgg tcctggttgg cccaaggaag ccaaccaaat
    14521 gccaaaactc attcaagact tttaccaaac aaaacctatc ttaggagtgt gtctgggaca
    14581 ccaagctatc gctgaaaccc tagggggaaa cttacgcttg gctaaacgtg tcatgcatgg
    14641 gagacaaagc accattgaaa cgcaaggccc tgctagtctt tttcgctccc tgccacaaga
    14701 gatcaccgtc atgcgttacc attccatcgt tgtggatcag ttaccaaaag gttttagcgt
    14761 aactgctaga gactgtgacg atcaagaaat catggccttt gaacaccaca ccctgccact
    14821 ttttgggcta caatttcacc cagaaagcat cggaactcct gatggcatga ccatgatcgc
    14881 caacttcatc gcagccattc cccgttaatc tcatccccca aaagaaagaa ggacagttac
    14941 atgcctgatc atttagcgct cagaatgcgc cccaaaacca tttcagaggt catcggacaa
    15001 aaacaccttg ttggcgaagg caaaatcatc cgtcgcatgg tggaagcaaa tagactatcg
    15061 tccatgatcc tctatggccc tccaggaatc ggtaaaactt ccattgcttc tgctattgct
    15121 ggtactacta gatatgcctt tcggaccttt aatgccacca ttgacagcaa aaaacgtctc
    15181 caagaaattg cggaagaagc taaattttct ggtggcttgg tattattact agacgagatt
    15241 catcgccttg acaagacaaa acaagatttt cttttacctt tacttgagaa tggcaccatt
    15301 atcatgattg gcgcaaccac tgagaacccc ttcttttcag tgacacccgc tatcagaagc
    15361 agggtccaga tttttgaatt ggaaccactt tctaacgagg atattaaaac agccattcag
    15421 ctagcaattt ccgacaaaga acggggattt ccttttttag tgacaattga tgatgaggcg
    15481 ctagatttca ttgttaccgc cacaaatgga gacctacgat ctgcttataa ttccttggat
    15541 ctagcagtca tgtctacttc tcctaatgaa gatagtagcc gtcatattag tctagagaca
    15601 atggaaaata gtctgcagcg tagttatatt accatggata aaaacgggga cgggcattat
    15661 gatgttttat ctgccttaca aaaatccatt agaggttctg atgtcaatgc tagcctgcac
    15721 tacgccgctc gtttagtaga agctggagac ttgcctagtt tagcaagacg attgaccatt
    15781 attgcttacg aagatattgg cttagccaac cctgacgccc aagttcatac cgtcaccgca
    15841 ctagatgccg ctcaacgcat tggttttcca gaagctcgta ttcttatcgc taacgtggtc
    15901 attgatttgg ctctatcacc taaatccaat tcagcttatt tagctatgga tgcagcttta
    15961 gctgatttga gaacatcagg taacctcccc attccaaggc atcttagaga cgggcattat
    16021 gctggcagta aggacctagg caatgccaaa gattacctct acccacacgc ctaccctgaa
    16081 aaatgggtca aacaacaata cctaccagat aaattagtag gacatcatta ttttgaagcc
    16141 aatgaaaccg gtaaatacga acgcgcactt ggttcaaata aagagcgtat tgataagctc
    16201 tcagactgac tgctagtttt aaaaatcgca accgatttca aaaatcgata attttgcttg
    16261 aaaaaatttt aaaaagtggt attataaaag tacagaaaca ttgctgtggc atacgaatcc
    16321 acatctaagt gttgaccgac tatttctttg tattttcggg aaacaaagac cctttctagt
    16381 ctgcaagccg tttcacgcgg acgcaacttc caagaaagag cgcgttaccc acctgcttat
    16441 actgcgcggg ctcaatacaa aaacgtggat caccggtgcc aatacagcat tcttgcctcc
    16501 ttagctcagt tggtagagca gtagactctt aatctatggg tcacaggttc gagccctgta
    16561 gggggtatac aactatacat gaaaaaacct ttgtttaaag gctttttttg cttgtcagaa
    16621 gaggatttgt tccatcattt atccccgcag taattttttt atggtatgat accaattaaa
    16681 gcgcttaaga ttaaggtgga gactgttatg aaaataattg agtacaaagg aaattacaga
    16741 gatgacctca tttttatggt cttggaagca aaaaatgcct taaggagaat tcccagcatt
    16801 aacccggatc ttttgacaat tgaaacaagt tatttggaaa aaggggattg tttttggcta
    16861 gcggtcaatg aagaaagtcg tgttattggc tcaatcgctt attcttctat tcccaaaaca
    16921 aatgaggtct tcttgcaccg tctcttcatc aaaccaagct taaaacatca aggcattggc
    16981 agtcaacttc tgacgtttgc agaagattat ctaaggaaaa tcggcaagag tcatgttcgt
    17041 atccacttag gtcaacctaa agaacaatgg tttgagtctt atcggtttta tcctaaacat
    17101 ggttatcaat tttatgatga cacgcatctc atcaagaaac taatctaaac acatttcata
    17161 ccctttatag tcatttcata cgtcattttt aggaggtatc cattgaaaat tgccatcatt
    17221 ggacatagtg gctctggaaa gtcaacctta gcacgatttt taggtcaaca ttaccattgt
    17281 gaggtggtcc atttagacca actacacttc tcatcgaact ggcaagaacg gtcagatcat
    17341 gacatgatag ctgatttatc aacatgcctg ctcaaacaag attggattat tgaggggaat
    17401 tatgccaatt gtctctatga agagcgcatg agtgaggcta atcacattat ctatgttaat
    17461 ttcagtcggt ttcattgtgt ctatcgagca tttaagcgtt atttaaacta taggggaaaa
    17521 acgcgtcctg atatggctga caactgtcag gaaaaatttg acgttgcgtt tgtgaaatgg
    17581 attctactag atggacgttc aagaaaccaa ttaaaaaaat accaatccgt tatccaaaaa
    17641 taccctcata aaaccattgt cttaacaaat caaaagcagc tcaatcacta tatgaataca
    17701 atcaactaat tagagtgaaa aactaaaata gccacgggta acccgggcta ttcagactga
    17761 aagcctttgt ttaaaggctt ttttgcttgt ttcataacta atcggcttaa ttaaaagcgt
    17821 caatccactt ttagtatcat gtctcaattt tcatttgata gtcagcaagt atatctacaa
    17881 aattgttaat gattatttct tttgaaagtg ttatat
//
LOCUS       DATXIG010000032        17756 bp    DNA     linear   BCT 28-DEC-2023
DEFINITION  TPA_asm: Streptococcus pyogenes strain MGAS11113
            SAMN08555799-rid19682583.denovo.032, whole genome shotgun sequence.
ACCESSION   DATXIG010000032 DATXIG010000000
VERSION     DATXIG010000032.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN08555799
            Sequence Read Archive: SRR6740495
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pyogenes
  ORGANISM  Streptococcus pyogenes
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 17756)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 17756)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-OCT-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-OCT-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001918058.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001918058.2
            Genome Coverage       :: 368x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/12/2023 11:23:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 1,806
            CDSs (total)                      :: 1,769
            Genes (coding)                    :: 1,722
            CDSs (with protein)               :: 1,722
            Genes (RNA)                       :: 37
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1, 1 (5S, 23S)
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 47
            CDSs (without protein)            :: 47
            Pseudo Genes (ambiguous residues) :: 0 of 47
            Pseudo Genes (frameshifted)       :: 30 of 47
            Pseudo Genes (incomplete)         :: 20 of 47
            Pseudo Genes (internal stop)      :: 9 of 47
            Pseudo Genes (multiple problems)  :: 11 of 47
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..17756
                     /organism="Streptococcus pyogenes"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN08555799-rid19682583.denovo.032"
                     /strain="MGAS11113"
                     /serotype="Emm28"
                     /isolation_source="normally sterile site"
                     /host="Homo sapiens"
                     /db_xref="taxon:1314"
                     /country="Finland"
                     /collection_date="2002"
                     /collected_by="National Public Health Laboratory"
     gene            complement(395..1840)
                     /locus_tag="VC122_001130"
     CDS             complement(395..1840)
                     /locus_tag="VC122_001130"
                     /EC_number="6.1.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012514887.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate--tRNA ligase"
                     /protein_id="HEP5641056.1"
                     /translation="MSKPIRVRYAPSPTGLLHIGNARTALFNYLYARRHGGTFIIRIE
                     DTDRKRHVEDGERSQLENLKWLGMDWDESPETHENYRQSERLALYQQYIDQLLAEGKA
                     YKSYVTEEELAAERERQEAAGETPRYINEFIGMSADEKAKYIAEREAAGIVPTVRLAV
                     NESGIYKWTDMVKGDIEFEGGNIGGDWVIQKKDGYPTYNFAVVVDDHDMQISHVIRGD
                     DHIANTPKQLMVYEALGWEAPEFGHMTLIINSETGKKLSKRDTNTLQFIEDYRKKGYM
                     PEAVFNFIALLGWNPGGEEEIFSREQLIALFDENRLSKSPAAFDQKKMDWMSNEYLKH
                     ADFETVYALCKPFLEEAGRLTEKAEKLVELYKPQLKSADEIIPLTDLFFSDFPELTEA
                     EKEVMAGETVSTVLQAFKAKLEAMSDEDFKPENIFPQIKAVQKETGIKGKNLFMPIRI
                     AVSGEMHGPELPNTIYLLGRDKSIEHIKNML"
     gene            complement(2023..2733)
                     /locus_tag="VC122_001131"
     CDS             complement(2023..2733)
                     /locus_tag="VC122_001131"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011888578.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR00266 family protein"
                     /protein_id="HEP5641057.1"
                     /translation="MVDNRMRFTIDQNMQFPLVEIDLEHGGSVYLQQGSMVYHTENVT
                     LNTKLNGKGSGLGKLVGAIGRSMVSGESMFITQAMSNGDGKLALAPNTPGQIVALELG
                     EKQYRLNDGAFLALDGSAQYKMERQNIGKALFGGQGGLFVMTTEGLGTLLANSFGSIK
                     KITLDGGTMTIDNAHVVAWSRELDYDIHLENGFMQSIGTGEGVVNTFRGHGEIYIQSL
                     NLEQFAGTLKRYLPTSSN"
     gene            complement(2864..3361)
                     /locus_tag="VC122_001132"
     CDS             complement(2864..3361)
                     /locus_tag="VC122_001132"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011888577.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbonic anhydrase"
                     /protein_id="HEP5641058.1"
                     /translation="MSYFEHFMAANQAYVALHGTAHLPLKPKTKVAIVTCMDSRLHVA
                     QALGLALGDAHILRNAGGRVTEDMIRSLVISQQQMGTREIVVLHHTDCGAQTFTNEGF
                     AKHIHEHLGVDVSGQDFLPFQDVEDSVRKDMAKLRASSLIPDDVVINGAVYDVATGKM
                     TQVME"
     gene            complement(3550..4911)
                     /gene="radA"
                     /locus_tag="VC122_001133"
     CDS             complement(3550..4911)
                     /gene="radA"
                     /locus_tag="VC122_001133"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010921866.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein RadA"
                     /protein_id="HEP5641059.1"
                     /translation="MAKKKATFICQECGYQSPKYLGRCPNCSAWSSFVEEVEVKEVKN
                     ARVSLAGEKSRPVKLKDVDNISYHRTQTDMSEFNRVLGGGVVPGSLILIGGDPGIGKS
                     TLLLQVSTQLANKGTVLYASGEESAEQIKLRSERLGDIDNEFYLYAETNMQAIRTEIE
                     NIKPDFLIIDSIQTIMSPDITGVQGSVSQVREVTAELMQLAKTNNIATFIVGHVTKEG
                     TLAGPRMLEHMVDTVLYFEGERHHTFRILRAVKNRFGSTNEIGIFEMQSGGLVEVLNP
                     SQVFLEERLDGATGSAVVVTMEGSRPILAEVQSLVTPTVFGNARRTTTGLDFNRVSLI
                     MAVLEKRCGLLLQNQDAYLKSAGGVKLDEPAIDLAVAVAIASSYKEKPTSPQEAFLGE
                     IGLTGEIRRVTRIEQRINEAAKLGFTKVYAPKNALQGIDIPQGIEVVGVTTVGQVLKA
                     VFS"
     gene            complement(4992..5438)
                     /locus_tag="VC122_001134"
     CDS             complement(4992..5438)
                     /locus_tag="VC122_001134"
                     /EC_number="3.6.1.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002986111.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dUTP diphosphatase"
                     /protein_id="HEP5641060.1"
                     /translation="MTKIRGFELVSSFTNPDLLPKRETTHAAGYDLSVAEAVTIAPGE
                     IKLVPTGVKAYMQDGEVLYLYDRSSNPRKKGIILINSVGVIDADYYGNEANEGHIFAQ
                     MQNITDHPVTLAVGERIVQGVFMPFLIADGDQARGERTGGFGSTGQ"
     gene            complement(5548..6315)
                     /locus_tag="VC122_001135"
     CDS             complement(5548..6315)
                     /locus_tag="VC122_001135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002986113.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="epoxyqueuosine reductase QueH"
                     /protein_id="HEP5641061.1"
                     /translation="MIDLQEILANMNPNQKINYDRVMQQMAKVWAKESVRPSILMHVC
                     CAPCSTYTLEYLTQFADITVYFANSNIHPKDEYHRRAYVTQQFVSEFNAKTGNTVQFL
                     EADYVPNEYVRQVRGLEEEPEGGDRCRVCFDYRLDKTAQKAVELGFDYFASALTISPH
                     KNSQTINDVGIDVQKVYTTKYLPSDFKKNNGYRRSVEMCEEYDIYRQCYCGCVYAAKM
                     QGIDLVQVKKDAKAFMADKDLDNDFTHIRFSYRGDEM"
     gene            complement(6433..8217)
                     /locus_tag="VC122_001136"
     CDS             complement(6433..8217)
                     /locus_tag="VC122_001136"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000859866.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEP5641062.1"
                     /translation="MKTARFFWFYFKCYRFSFTVIAVAVILATYLQVKAPVFLGESLT
                     ELGKIGQAYYVAKMSGQTHFSPDLSAFNAVMFKLLMTYFFTVLANLIYSFLLTRVVSH
                     STNRMRKGLFGKLERLTVAFFDRHKDGEILSRFTSDLDNIQNSLNQSLVQVVTNIALY
                     IGLVWMMFRQDSRLALLTIASTPVALIFLVINIRLARKYTNIQQQEVSALNAFMDETI
                     SGQKAIIVQGVQEDTVTAFLKHNERVRQATFKRRLFSGQLFPVMNGMSLINTAIVIFV
                     GSTIVLSDKSMPAAAALGLVVTFVQYSQQYYQPMMQIASSWGELQLAFTGAHRIQEMF
                     DETEEVRPQNAPAFTSLKEAVAINHVDFGYLPGQKVLSDVSIVAPKGKMIAVVGPTGS
                     GKTTIMNLINRFYDVDAGSITFDGRDIRDYDLDSLRQKVGIVLQESVLFSGTITDNIR
                     FGDQTISQDMVETAARATHIHDFIMSLPKGYNTYVSDDDNVFSTGQKQLISIARTLLT
                     DPEVLILDEATSNVDTVTESKIQRAMEAIVAGRTSFVIAHRLKTILNADHIIVLKDGK
                     VIEQGNHHELLHQKGFYAELYHNQFVFE"
     gene            complement(8220..9926)
                     /locus_tag="VC122_001137"
     CDS             complement(8220..9926)
                     /locus_tag="VC122_001137"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011888575.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEP5641063.1"
                     /translation="MLKDAILRYKWYALGSFFMVIGVVASSLLQPHYLKSVLSAVIQN
                     NQEKIYEVGKPLLIIALLGLMSGAINTVLAAKIAQGVSADMREKTFRKIQDFSYANIE
                     AFNAGNLVVRLTNDINQIQSLVMMMFQILFRLPILFIGAFIMAVQTFPQLWWVIVVMV
                     ILIALIMGLVMRQMGPRFGKFQRLMDKINRIAKENLRGVRVVKSFVQEQQQYTKFKET
                     SNDLLALNLSIGYGFSLMQPALMLVSYLAVYVSINVVSTMVETDPTVIGNIASFMTYM
                     MQIMFSIIVVGSMGMQVSRAFVSMARIRQILSTEPAMTFENEKEETISGSIVFDDVSF
                     TYPNDDEPTLKHISFAIESGQMVGIVGATGSGKSTLAQLIPRLFDPQDGQILLGGKPI
                     KTLSQTTLRQSVSIVLQKAILFSGTIADNLRQGSAKADIDAMQKAAQIAQAKEFIDRM
                     DSRYESQVEERGSNLSGGQKQRLSIARGVINHPKILILDDSTSALDAKSEKRVQEALS
                     HKLEGTTTVIVAQKISSVVKADKILVLDQGQLIGEGTHAELVANNAIYREIYETQKGR
                     EE"
     gene            complement(9919..10368)
                     /locus_tag="VC122_001138"
     CDS             complement(9919..10368)
                     /locus_tag="VC122_001138"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002986120.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family transcriptional regulator"
                     /protein_id="HEP5641064.1"
                     /translation="MSQVIGDLRELIHQIEQISDEIAKKYDVEHLAGPQGYVLVFLAK
                     HQNQEIFVKDIEKQLRISKSVASHLVKRMVKNGFINVMPSQVDKRYKQVVLAQVGRDK
                     LPLLRECRKDIEHYFLKEITKEELLTAKKVIEQLKQNMLTYKGDNDA"
     gene            10666..11682
                     /locus_tag="VC122_001139"
     CDS             10666..11682
                     /locus_tag="VC122_001139"
                     /EC_number="1.1.1.94"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011184140.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-dependent glycerol-3-phosphate
                     dehydrogenase"
                     /protein_id="HEP5641065.1"
                     /translation="MTKQKVAILGPGSWGTALSQVLNDNGHDVRLWGNIPDQIEEINT
                     KHTNRHYFKDIVLDKNITATLDLGQALSDVDAVLFVVPTKVTRLVARQVAAILDHKVV
                     VMHASKGLEPETHERLSTILEEEIPAHFRSEVVVVSGPSHAEETIVRDITLITAASKD
                     IEAAKYVQSLFSNHYFRLYTNTDVIGVETAGALKNIIAVGAGALHGLGYGDNAKAAVI
                     TRGLAEITRLGVKLGADPLTYSGLSGVGDLIVTGTSVHSRNWRAGAALGRGEKLEDIE
                     HNMGMVIEGIATTKVAYEIAQDLGVYMPITTAIYKSIYEGADIKESILGMMSNEFRSE
                     NEWH"
     gene            11715..12614
                     /gene="galU"
                     /locus_tag="VC122_001140"
     CDS             11715..12614
                     /gene="galU"
                     /locus_tag="VC122_001140"
                     /EC_number="2.7.7.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002986125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UTP--glucose-1-phosphate uridylyltransferase
                     GalU"
                     /protein_id="HEP5641066.1"
                     /translation="MTKVRKAIIPAAGLGTRFLPATKALAKEMLPIVDKPTIQFIVEE
                     ALKSGIEEILIVTGKSKRSIEDHFDSNFELEYNLQAKGKIELLKLVDETTSINLHFIR
                     QSHPRGLGDAVLQAKTFVGNEPFVVMLGDDLMDITNPNVKPLTKQLIDDYEETHAATI
                     AVMRVPHEDVSNYGIIAPQAKAVKGLYSVDTFVEKPQPQDAPSDLAIIGRYLLTPEIF
                     SILEKQEPGAGNEVQLTDAIDTLNKTQRVFAREFKGKRYDVGDKFGFMKTSLDYALKH
                     PQVKDDLKAYIIQLGKALEKTKP"
     gene            complement(12713..13384)
                     /locus_tag="VC122_001141"
     CDS             complement(12713..13384)
                     /locus_tag="VC122_001141"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011284482.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhomboid family intramembrane serine protease"
                     /protein_id="HEP5641067.1"
                     /translation="MTRLLKRYPITIFLLGLTGLIFIAMQVVYGHLATGAQAIYQVGG
                     MFGLLVKAMPDQLWRLVTPIFIHIGFGHFFVNGLTLYFVGQIVEDLWGSRRFLLLYVL
                     SGVMGNAFTFWLSPETVAAGASTSLFGLFAAIVVLSFLGKNQALKDLGKSYQTLIVVN
                     LLMNLFMPNVSMAGHIGGVVGGVLLSIVFPTKMRVITVKKTKRMLALVSYGIILVGVL
                     VLGFL"
     gene            14072..15580
                     /locus_tag="VC122_001142"
     CDS             14072..15580
                     /locus_tag="VC122_001142"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002986134.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HEP5641068.1"
                     /translation="MLDYYLESDIIDQKILLAILLTEKELTMAAACSQTTLTALKIKQ
                     YLRQFNALFKGYLRIELIKSRIYCEVLNDKREAFFYDIFALSDTLKMLTFLLLDNPKH
                     KSIAVYTRKQGISQSKAYRLIHKLKHYLQDIGLNIVDNTVIGDELKIRYLIALLHKEY
                     GIILYDIQPADIETIHAFIFAAQKNLQPSAFLDRRFLFFDVLLMLTWKRHRYPVHLPH
                     LALFEHLKSLPIFDNIKTIAVDELAPRTRVTFSSDDFDYLFLIYLTTDNSFLSGYWTS
                     HRQQQLYHLITKDPDYHLLIHRLQALVGKYSDIHEHIPNLIPFFKRTLYNLQTLITFD
                     GYYFDQYQGNMLLLDKLETVIKDWLYDTGRQGSISSGHLHLMCLYLEQILESSIAPIN
                     ITVIESQETVGNVIGNFITSTIPSYKVELSRVNILSDNIYPYDKPVDLVVTSQKLLPF
                     LKELGVFPKETRLFGLSLDCIQQQREDLIKTILALHQNHYQKRLEELWRVPS"
     gene            complement(15929..17278)
                     /locus_tag="VC122_001143"
     CDS             complement(15929..17278)
                     /locus_tag="VC122_001143"
                     /EC_number="5.3.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017648691.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-6-phosphate isomerase"
                     /protein_id="HEP5641069.1"
                     /translation="MSHITFDYSKVLESFAGQHEIDFLQGQVTEADKLLREGTGPGSD
                     FLGWLDLPENYDKEEFARILTAAEKIKADSEVLVVIGIGGSYLGAKAAIDFLNHHFAN
                     LQTAKERKAPQILYAGNSISSTYLADLVEYVQDKEFSVNVISKSGTTTEPAIAFRVFK
                     ELLVKKYGQEEANKRIYATTDKVKGAVKLEADANNWETFVVPDNVGGRFSVLTAVGLL
                     PIAASGADITALMEGANAARKDLSSDKISENIAYQYAAVRNVLYRKGYITEILANYEP
                     SLQYFGEWWKQLAGESEGKDQKGIYPTSANFSTDLHSLGQFIQEGYRNLFETVIRVDN
                     PRKNVIIPELAEDLDGLGYLQGKDVDFVNKKATDGVLLAHTDGGVPNMFVTLPAQDEF
                     TLGYTIYFFELAIAVSGYMNAVNPFDQPGVEAYKRNMFALLGKPGFEALSAELNARL"
BASE COUNT         5400 a         3773 c         3181 g         5402 t
ORIGIN      
        1 ttttttctcc tttccaactt tattatttta gcatatttta tgacaatgat tacaatatta
       61 tttgattaca attgattagc taagtgaata ctaaactagt ctctttcagc atataccaaa
      121 aaaatattgt acactaattg attcataaaa accatttctt taaatcacac taatcaaaac
      181 ctcaccgttt gctgtcatta tcacgtaagc atctcaccac ctctctttca aatccaatct
      241 ccaaaataga aataaactat actatttttt actttaccaa ggtagcaagc tcagataacg
      301 ataaaaaaga ctgaagaact taatcttcaa tctttcgttg tcgtgacttt ttaactcctt
      361 aaagcgttta aaaataaagg tgaacgatac agccttatag catgtttttg atgtgctcaa
      421 tggatttatc acgtcccaat aaataaatcg tatttggtaa ttctgggcca tgcatttcac
      481 cagatactgc aatacgaata ggcataaaga ggtttttccc tttgattcct gtttcttttt
      541 gaacagcctt aatttgtggg aaaatatttt ctggtttgaa gtcttcatct gacatagctt
      601 ccaacttagc cttgaaagct tgcaagactg tcgaaactgt ttcacctgcc atcacttctt
      661 tttcggcttc tgttaattca gggaaatcag aaaagaaaag gtcagtcaat ggaataatct
      721 catctgcaga ttttaattga ggtttgtata attctactag cttctcagct ttttctgtca
      781 aacggcctgc ctcttcaagg aaaggtttgc agagggcata aactgtttca aaatccgcat
      841 gtttaagata ctcattgctc atccaatcca ttttcttctg gtcaaaggca gctggcgact
      901 tgctaaggcg attttcatca aaaagggcaa tcagttgctc acgagagaag atttcctctt
      961 ctccgccagg attccagcca agaagggcga tgaagttaaa gacagcttca ggcatataac
     1021 cttttttgcg gtaatcttca atgaattgga gggtattggt gtcgcgtttt gataattttt
     1081 tacccgtttc agaattgatg ataagagtca tatgaccaaa ctctggcgct tcccagccaa
     1141 gagcttcata gaccatcaat tgttttggcg tattggcaat atggtcatcg ccacggataa
     1201 cgtgagaaat ttgcatatca tgatcatcaa caacaacggc aaagttataa gttgggtagc
     1261 catctttctt ttgaataacc cagtcaccac cgatgttacc accttcaaac tcgatatcac
     1321 ctttaaccat gtcagtccat ttgtaaatac cggactcatt aacagcaaga cggactgttg
     1381 gaacgatacc tgctgcttca cgctcagcaa tgtacttagc tttttcgtca gctgacatac
     1441 caataaattc attgatatag cgaggcgttt cgccagctgc ttcttggcgt tcacgctctg
     1501 ctgccaattc ttcctcagtc acgtaagact tataggcttt tccttcagct aacaattgat
     1561 caatatattg ttgataaagt gctagacgtt cagactggcg gtaattttca tgcgtttctg
     1621 ggctttcatc ccaatccatt cctaaccatt ttaggttttc aagttgagaa cgttcaccat
     1681 cttcaacatg acgtttacgg tcagtatctt caatacgaat gataaaagta cctccgtgac
     1741 gacgggcata aaggtaatta aatagggctg tgcgggcatt cccgatatgt aaaagccctg
     1801 ttggacttgg tgcataacgc acacgaattg gtttagacat gcttcttctc ctgttacata
     1861 tatcactcag aaacctgtgg cacgaaaaac aagcaacaaa tgatgatttg agcttgccaa
     1921 gaccttttca gacttcttct aaaatctttc ttattatagc atattttact tgcttaggcg
     1981 aaaaaaaatc agccctaaaa agctgatttt atgttatttt gactaatttg aactagttgg
     2041 tagataacgt ttaagagtcc cagcaaattg ctcaaggtta agactttgga tataaatctc
     2101 accatgacct ctaaaggtat taacaactcc ttcacctgta ccaattgact gcataaagcc
     2161 attttctaaa tgaatatcat aatcaagttc tctgctccat gctacaacat gggcattatc
     2221 aatagtcatt gtgccaccat caagtgtgat tttcttgata gatccaaacg agttagcaag
     2281 taaggtacca aggccttcag ttgtcatgac aaaaagtccg ccttgaccac caaaaagtgc
     2341 ttttccaata ttttgacgtt ccattttgta ctgtgctgaa ccatctaggg ctagaaaggc
     2401 accatcattt agacgatatt gtttttcacc tagttctaaa gcaacaatct gtcctggtgt
     2461 attgggtgct aatgctaatt taccatctcc atttgacata gcttgtgtaa tgaacattga
     2521 ttcaccagaa accatggaac ggccgatggc tccaactaat tttcctaaac cagatccctt
     2581 accatttaat ttggtattca aggttacatt ctcagtatga taaaccatac ttccttgttg
     2641 taagtaaact gatcccccat gttccagatc aatctcaact agtggaaatt gcatgttttg
     2701 atcaatcgta aatctcattc tattgtcgac catcttactc tccttgtttg tatgaataac
     2761 ttgatacaat tattataacg cttccctatt tctttgtcta atattttcaa taatttttaa
     2821 aaataaaaaa gagctgaatt cagctctaca acttggctac taactattcc attacttgtg
     2881 tcatctttcc agtagccaca tcgtagacgg caccgttaat aacgacatca tcagggataa
     2941 gagaggaggc tcttagctta gccatatcct tgcggacact gtcttccaca tcttggaagg
     3001 gaaggaaatc ttgccctgaa acatccacac ctaagtgctc atgaatatgc ttggcaaagc
     3061 cttcattagt aaaagtctgc gcgccacaat ctgtatggtg gagaaccaca atctctcgcg
     3121 ttcccatttg ctgctgagaa ataaccagcg accgaatcat gtcttcggtg acacgtccgc
     3181 cagcattcct taaaatatga gcatctccta gagccagccc cagagcttgg gccacatgta
     3241 gccgtgaatc catacaagtc acaatcgcta ctttagtctt gggttttaag ggcagatgag
     3301 ctgtcccatg caaggccaca taagcctgat tggcagccat aaaatgttca aaataagaca
     3361 tcaataaact ccttttgaga aacagcagag ctatttttgc tgttagtttg tcttatctta
     3421 acatctttta tggaaaatag ctggattaac ctcttagacg gtcggtatcg ttcgaaaagt
     3481 taaaaaaaca aatgattggg atactttctt gacaaatttc ctcctcattt gctcttagtt
     3541 tctgatcctc tagctaaaaa ctgcctttaa aacctgacca accgtagtca ctccaacaac
     3601 ctcaatacct tgagggatat caatgccttg caaggcattt ttaggagcat agaccttggt
     3661 aaagccaagc tttgctgctt cattaatccg ttgctcaata cgggtcactc gccgaatctc
     3721 acctgttaac ccgatctcgc ctaagaaagc ttcttgaggg ctggttggtt tttccttata
     3781 actggatgcg atagccacag cgactgccaa atcaatggca ggttcgtcta gtttgacacc
     3841 accagccgac ttgaggtagg catcttgatt ttgtaagagt aaaccacaac gcttttctaa
     3901 aactgccatt attaggctta cgcgattgaa atcaagtccc gtagtggttc gtctggcatt
     3961 gccaaaaacg gttggtgtca ctaaagattg cacctctgct aagataggac gacttccttc
     4021 catcgttaca acgacagccg atcctgttgc cccatctaag cgttcctcta aaaagacttg
     4081 actaggattt aagacctcta ccaagccgcc agactgcatt tcaaagatgc caatctcgtt
     4141 agtggagcca aagcggtttt tcacagcccg aagaatgcga aaggtgtgat gcctttcccc
     4201 ttcaaagtag agaacggtat ccaccatgtg ttctaacata cgaggcccag ccaaggttcc
     4261 ttccttggtg acatgaccca caataaaggt tgcaatgttg ttcgtctttg caagctgcat
     4321 caattctgca gtgacttctc tcacctgact gactgatcct tgaacacctg taatatcagg
     4381 gctcatgatg gtctgaatgg agtcaataat taaaaaatct ggtttaatat tttcaatttc
     4441 tgtgcgaata gcttgcatat tggtttcggc atagaggtaa aattcattgt caatatcacc
     4501 cagacgctca ctccgcagtt taatctgctc tgctgattct tccccagacg catagagaac
     4561 cgttccctta ttagcgagct gcgtagatac ctgcaagagt aaggtggatt ttccaatacc
     4621 tggatcacca ccaattaaaa tcaagcttcc aggaaccaca ccccctccaa gaactctatt
     4681 aaattcagac atgtcagtct gtgttctgtg ataactaata ttatccacat cttttagttt
     4741 aaccggtctg gacttttctc cagctagact aacacgggca tttttgactt ctttgacctc
     4801 tacttcttcc acaaaagaag accaggccga gcaatttgga cagcgcccta aatactttgg
     4861 cgactggtag ccacactcct gacagataaa ggttgctttt ttctttgcca tagtaatctc
     4921 cctcccctag acaagacaaa cccactctta aagaataggt ctactgtcat aagcattttt
     4981 gttaaatcgc tttactgccc tgtcgaacca aaaccacctg ttcgttcacc tctggcttgg
     5041 tcaccatcag caattaaaaa aggcataaaa accccctgaa cgatcctctc acccacagct
     5101 aatgtcacag ggtgatctgt aatgttttgc atttgtgcaa aaatatgtcc ttcattagcc
     5161 tcatttccat aataatctgc atcaatcaca cctactgaat taatcaggat aatccctttt
     5221 tttcgtggat ttgacgaacg atcatagagg taaagcacct caccatcttg catgtaggcc
     5281 ttaacccctg ttggtaccaa cttaatctcc cctggggcaa tggttacagc ctcagcaaca
     5341 gacaggtcat aaccagctgc atgagtcgtc tcacgttttg gtagtaaatc aggattagta
     5401 aagcttgaaa caagctcgaa accacgaatt tttgtcatta tgataggtat atcctttcaa
     5461 gtcaatcata tcctcttatt atagactatt cgtaaaagaa tagcaaaaag gcggtcaact
     5521 ttaaacggac cacttttaat catgccatca catctcatcc ccacgataac taaagcggat
     5581 gtgggtgaaa tcattatcca aatccttgtc tgccataaaa gctttggcat cttttttcac
     5641 ttgaaccaag tcaatacctt gcattttggc agcatagaca cagccacaat agcattggcg
     5701 gtaaatgtca tactcctcac acatttcaac ggatctgcga taaccgttgt ttttcttgaa
     5761 atcacttggc aggtatttgg tggtgtaaac cttttgcaca tcgatgccca catcattaat
     5821 ggtttgcgaa tttttatgag gactgatggt tagggcactg gcaaagtagt caaaacctag
     5881 ttccactgct ttttgagcgg tcttgtctag tcggtaatca aaacagacac ggcaacggtc
     5941 cccaccttct ggttcttctt ctaaacctcg cacttgtcga acgtactcat tggggacata
     6001 gtccgcttcg agaaactgca cggtgttgcc tgttttagcg ttgaactcgg agacaaactg
     6061 ttgtgtcaca taggctctgc gatggtattc gtccttaggg tgaatattag aattggcaaa
     6121 gtaaaccgta atgtcagcaa actgcgtcag gtattctagg gtgtaagtgg agcatggggc
     6181 gcagcagaca tgcattaaaa tactgggacg cacggattcc ttagcccaaa ccttggccat
     6241 ctgttgcatg accctgtcat aatttatctt ttgattggga ttcatgttag cgagaatctc
     6301 ttgtaaatca atcattgtgg ctactttcta attgtttttt ctcttgcttg ctattatacc
     6361 atatcaggaa agaaagcttg actgctttat tgttcaccaa aaaactccgc taagacgcgg
     6421 agctgatacc atctattcaa agacaaattg attgtgatac aattcggcat aaaagccttt
     6481 ttgatgcaat agctcatgat gatttccttg ctcaatgacc ttaccatctt tcaacacaat
     6541 aatgtgatcg gcatttaaaa tggttttgag gcggtgagca atgacaaagc tggttcgacc
     6601 tgccacgata gcttccatgg cccgttgaat tttactttcg gtaactgtat caacatttga
     6661 agtggcctca tccaaaatca acacttcagg gtcagtcagt agtgtcctag caatagaaat
     6721 caactgcttt tgacctgttg aaaagacatt gtcatcatct gagacatagg tattgtaccc
     6781 ttttggtaag gacatgataa agtcatgaat atgggtcgca cgcgcagcag tttcaaccat
     6841 gtcttgacta atggtctgat caccaaaacg aatattatcc gtaatggttc ctgaaaaaag
     6901 aactgactct tgcaacacaa tccctacctt ttgacgaaga ctatccaaat cgtagtcacg
     6961 aatatcacgg ccatcaaagg taatcgaacc tgcatccaca tcgtagaaac ggttaatcaa
     7021 gttcataata gtggtctttc cagaacctgt cggtccaacc acggcaatca ttttgccctt
     7081 gggtgcaacg attgacacat ctgataaaac tttttgccca ggaagatacc caaaatcgac
     7141 gtggttaatt gccactgctt cttttaagct ggtgaacgct ggtgcatttt gtggacgaac
     7201 ttcttcggtt tcatcaaaca tttcttgaat acgatgagca ccggtaaagg ccagctgcaa
     7261 ttctccccaa ctagacgcga tttgcatcat gggttggtaa tattgctggg aatattgtac
     7321 aaaagtaacc actaaaccaa gcgctgccgc tgctggcata gatttgtcac tgaggacaat
     7381 tgttgaaccg acaaaaatca cgatagccgt gttaataagg ctcattccat tcatgactgg
     7441 aaataattgt cctgagaaca gacggcgttt gaaggtggct tgtcgaaccc tttcgttatg
     7501 ttttaaaaag gctgtcaccg tatcttcttg gacaccttgt acaataatag ccttttgtcc
     7561 tgaaatggtt tcatccataa aagcatttaa agcactgact tcttgctgtt ggatattggt
     7621 gtattttctt gccaaacgga tgttaatcac taaaaaaatg agagcaactg gggttgatgc
     7681 gatggttaac aaagctaaac ggctatcttg cctaaacatc atccagacca ggccgatgta
     7741 aagggcaata ttagtcacca cttgaaccaa ggattggttc agcgagtttt gaatattatc
     7801 caaatcactc gtgaaacgag aaagaatctc cccatcttta tggcggtcaa aaaaggcgac
     7861 ggttaaacgt tctaatttac caaacaagcc cttgcgcatg cggttggtcg aatgtgagac
     7921 aacacgtgtg agtaagaaac tatatattag attagctaaa acagtaaaga aataagtcat
     7981 caaaagctta aacatcacgg cattaaaagc tgataaatca gggctaaaat gtgtctgacc
     8041 actcatctta gcaacgtaat aagcctgacc gatttttccc aactcagtca aggactcccc
     8101 taagaagaca ggagctttta cttgtaaata agttgctaag ataacggcaa cagcaatgac
     8161 agtaaacgag aaacgatagc atttaaaata aaaccagaaa aaacgtgctg ttttcatcac
     8221 tactcctccc ttcctttttg tgtctcataa atttcacgat aaatcgcatt attagccacc
     8281 aactctgcgt gtgttccttc tccaattaac tgtccttggt caaggactaa gatcttatct
     8341 gccttaacta ccgatgaaat tttttgagca acaataactg tagttgtacc ttctaattta
     8401 tgacttaagg cttcttgaac gcgtttttca gattttgcat caagagctga agtggaatcg
     8461 tctaatataa gaattttggg atgattaatg acaccacgtg caatagacag acgttgcttt
     8521 tgtccgccag acaagttact acctcgctct tcaacttggc tctcataacg gctatccata
     8581 cgatcaataa attcttttgc ttgcgcgatt tgagcggctt tttgcatagc atcaatatca
     8641 gcttttgctg agccttggcg aaggttatca gctatcgttc ctgaaaagag aatagccttt
     8701 tgcaacacga tggaaaccga ttggcgcagg gttgtttgac ttagggtttt aatcggcttt
     8761 cctcctaaca aaatttggcc atcttgagga tcaaataagc gagggatgag ctgtgcaagc
     8821 gttgatttcc cagatcctgt tgcaccaacg atgccgacca tttgcccaga ttcaatagca
     8881 aaagaaatgt gcttcaaggt tggctcatca tcattaggat atgtaaaact aacatcgtca
     8941 aatacaatgc ttcctgagat ggtttcttcc ttttcatttt caaatgtcat ggcaggttct
     9001 gtagacaata tttgcctaat tctagccata gaaacaaaag cacgactaac ttgcatcccc
     9061 atggaaccaa ccacaataat tgaaaacata atttgcatca tataagtcat gaaagaggca
     9121 atattcccaa tcactgttgg atctgtttcg accatggtcg aaacaacatt tatagaaaca
     9181 taaactgcca aataagacac caacattaaa gccggctgca ttagtgaaaa accataacca
     9241 atagataaat ttaacgccaa aagatcattg gacgtttctt taaatttagt gtattgttgt
     9301 tgttcttgta caaatgattt aaccacgcga acgccacgga gattttcctt agcgatgcga
     9361 ttaatcttat ccattaaccg ttgaaattta ccaaaacgag gacccatttg acgcatcacc
     9421 aaacccatga tgagggcaat taagataacc attacaacaa tgacccacca taattgaggg
     9481 aatgtctgaa ctgccataat aaacgcgcca ataaaaagga tcggcaaacg aaagagaatt
     9541 tgaaacatca tcatgactaa actttggatt tgattgatat cattagttag cctaaccaca
     9601 agattaccag cattaaaagc ttcaatattg gcatatgaaa agtcttggat ttttcgaaag
     9661 gtcttctccc tcatatcagc agatacacct tgagctattt tagcagctaa aaccgtatta
     9721 atcgctccag acattaaccc taaaagggca ataataagta atggtttccc aacctcataa
     9781 attttctctt gattattttg aatcacagcg cttagcacag atttaagata atgtggttgc
     9841 aagagactac ttgcaacaac cccaataacc ataaaaaatg agcctaaagc ataccattta
     9901 taacgtaaga ttgcgtcttt aagcatcgtt gtctccttta taagttagca tattttgctt
     9961 gagctgttca attacttttt tcgctgtcag caactcttct tttgtaattt cttttaaaaa
    10021 atagtgctcg atatccttac gacactcccg caacaaaggc aatttatctc taccaacctg
    10081 cgctaacact acttgcttat aacgcttatc cacttgggaa ggcatcacat tgataaaccc
    10141 atttttgacc atacgtttca ctaaatgact agcaactgac tttgagatac gaagttgttt
    10201 ttcaatatct ttgacaaata tttcttgatt ttggtgttta gctaaaaaaa caagaacata
    10261 accttgagga cctgctagat gttctacatc atattttttt gcaatctcat cactaatttg
    10321 ttcgatttga tgtatcaatt cacgtaaatc acctatcact tgtgacatct cttccctcct
    10381 tattttcttt aatagtttct atgataagta ttttagttct ctagataact attgtcaatt
    10441 aaaatatctt tatttttaaa aataagtttc cgaacttaaa taactaaaaa gaacagggca
    10501 aagccatgac aacagctcac ctgatgaagc ttattaaaca acattattaa tatagctagc
    10561 tctcttggga acgccttatt agccaagacc atataccttt tctaatagag ctaatcaagg
    10621 tgttatgtta taatagagtg actagctctt gaaggagaat ttagaatgac aaaacaaaaa
    10681 gtagctattt taggacctgg ttcttgggga actgcccttt ctcaggttct taatgacaat
    10741 ggccatgacg tccgtttatg gggaaatatt cctgatcaaa ttgaagagat aaataccaag
    10801 cataccaata gacactactt caaagacatt gtgttagaca aaaacattac ggcaactctt
    10861 gatttaggtc aagccctatc agatgttgat gcggtgcttt ttgttgtgcc aactaaagta
    10921 acacgattag ttgctagaca agttgccgcc attttagatc ataaagtagt ggtcatgcat
    10981 gcctcaaaag gtctagaacc agagacccac gaacgtttat caactatcct agaagaagaa
    11041 attccagctc attttcgtag tgaagtggtc gttgtatcag gtcctagtca tgctgaagaa
    11101 actatcgttc gcgatattac tctcatcacg gcagcctcaa aagatattga agctgcaaag
    11161 tatgttcagt ccctctttag taaccactac ttccgacttt ataccaatac cgacgtcatc
    11221 ggagttgaaa cagctggtgc cctcaaaaac attattgctg ttggagcagg agctctccat
    11281 ggacttgggt atggagataa tgccaaagca gcggtaatta ctcgtgggtt agctgaaatc
    11341 acccgtctag gcgtcaagct tggtgctgac ccgttaacct acagtgggtt atctggtgtt
    11401 ggtgatctaa ttgtaacagg tacttctgtc cattcacgta actggcgggc tggagctgcc
    11461 cttggacgtg gtgaaaaatt agaagatatt gaacacaaca tgggtatggt tatcgaaggc
    11521 atcgcaacaa ctaaggtagc ctatgaaatt gcccaagacc tcggagttta catgccaatt
    11581 acaacagcta tttataagtc catctacgag ggcgctgata tcaaagaaag catcctcggc
    11641 atgatgtcaa atgaatttcg ttctgaaaat gaatggcatt aagccattcg tcactaaaat
    11701 aagaggaggt gctgatgacc aaagtcagaa aagctattat tcccgcagca ggtttaggca
    11761 cacgtttttt acctgcaacc aaggcccttg ccaaggagat gctacctatt gttgataaac
    11821 caactattca atttatcgtt gaagaagctc taaaatcagg tatcgaagaa attcttatcg
    11881 ttactggaaa atccaaacgc tccatagaag atcattttga ttctaatttt gaattagaat
    11941 ataatttaca ggctaaagga aaaatcgaac ttctaaagtt agtagacgag accacttcta
    12001 ttaaccttca ctttattcga cagagtcatc caagaggatt aggagacgca gttcttcaag
    12061 caaagacctt tgttggcaat gaaccttttg ttgtcatgct aggtgatgat ctgatggata
    12121 ttacaaatcc aaatgtaaaa ccattaacca aacaattgat cgatgactat gaagaaactc
    12181 atgctgcaac tattgccgtc atgagggttc ctcacgagga tgtctctaac tacggtatca
    12241 ttgctcctca agcaaaagca gtaaaaggac tctatagtgt ggatactttt gttgaaaaac
    12301 cacaacctca agatgcacct agtgatttgg caattattgg acgttacttg ctcacaccag
    12361 aaatttttag tattttagaa aaacaagaac ctggtgcagg caatgaagtt caactgactg
    12421 atgctattga taccctcaat aagacacagc gtgtctttgc tcgtgagttt aaagggaaac
    12481 gctatgatgt tggcgataaa tttgggttta tgaaaacttc tttggattat gccctgaaac
    12541 accctcaagt caaagatgac ttgaaagcct acatcatcca actgggcaaa gcactagaga
    12601 aaacaaaacc ataaaaataa ggagggaaaa tccctcctta tttttatggt ttttgagtcg
    12661 tttcatccta ctagccatct gagaagtaac agtgctaacc actcacttat ttttataaaa
    12721 acccaagcac taacacacca accaaaataa taccatagct cactaaagcc aacattcgct
    12781 tggttttctt cactgttatc actctcatct tagttggaaa tactatactc agtaacacac
    12841 cacccacgac accgccaata tggcctgcca tactcacgtt tggcataaaa agattcatca
    12901 gtaaattgac tacaattaac gtctggtatg acttgcccaa atctttcaac gcttggttct
    12961 tacccaaaaa actcaatact acaatagcag caaaaagtcc aaataacgac gtcgatgctc
    13021 ctgcagctac tgtttcagga ctcaaccaaa aagtaaaggc attgcccata acacctgata
    13081 aaacataaag taataaaaaa cggcgtgaac cccagaggtc ttcgacaatt tgaccaacaa
    13141 aatatagtgt taacccattg acaaaaaaat gaccaaagcc gatatgaata aatatcggag
    13201 tcaccaatcg ccacaactga tctggcattg cttttactag taatccaaac atacctccaa
    13261 cttgataaat agcttgagca ccagttgcta aatgtccata taccacttgc attgctataa
    13321 aaattaagcc tgttaaccct aaaaggaaaa tagtaatagg atatcgtttt aataaccgtg
    13381 tcatctaaga taaacctcct aaacagcaat atcataaatc gtttgatttg gcaatcgtat
    13441 tggcccgctg ataagcttga ttgctaacaa catccactaa taaagcttga tcattttgac
    13501 gttttaagtc tacatccata gttcgtaaag actagaagag ttctttgaga atctcttttt
    13561 tcatcatctt cttatcatac cataaattag gaaaaaggtt caagactgac aagaaattag
    13621 ctctctcaaa aaagattaaa aataaaggca ttctaaagaa tcatttaaga aaactttaag
    13681 gttactatcg tagaatgaaa acatcctaaa aagcggttta cctaaaccgt tgggagaata
    13741 ggttttcccc ttgttttccc ttttcataaa tctcctgggc acctatcctc gtaggtgccc
    13801 tcccttgttt aaacctgacc agttaaccgg ctggttaggt ttttaaattt aaaaagtcaa
    13861 cagcaaaaat atgatatcac ccgcgtgttc ttagctctct caattatggg ctaaaaacac
    13921 gcatcaatca atctattgat gaagtttgct gctttttgat gatctttatt gtgactatac
    13981 tggtaatcct attgatttta gttgactttt acggtagatt cgctacaata tataggatta
    14041 tacaatttaa tttaaggaga gaaaaattac attgttagat tattatttgg aatccgatat
    14101 tattgaccag aaaatcctac tggctatttt gttgacagaa aaagaactga cgatggcagc
    14161 agcttgttct caaacaactt tgactgcttt aaaaataaag cagtatcttc gacaatttaa
    14221 cgccttattt aaagggtact tacgtattga gcttattaaa tctcgtattt attgtgaggt
    14281 attaaatgat aagcgagaag cattttttta tgatattttt gccttatcag atactctaaa
    14341 aatgttgaca ttcttattac tagataatcc caaacacaaa tcaatcgctg tttatacacg
    14401 aaaacaaggg atatcccagt caaaagctta tcgtcttatt cataaattaa aacattattt
    14461 acaagacatt ggtttaaata ttgtagacaa tactgttatc ggagatgaac ttaaaatacg
    14521 gtatttgatt gctcttttac ataaagagta cggcattata ttatatgata tacaacctgc
    14581 agacattgaa acgattcatg cttttatctt tgcagcacaa aaaaacttac aaccttctgc
    14641 ctttttagat agacgcttct tgttttttga tgtgctgttg atgttgactt ggaagcgcca
    14701 tcgctatcct gttcatttgc ctcatttagc tctttttgaa catctcaaat ccttgcctat
    14761 ttttgacaat atcaaaacca ttgccgtgga tgagctagca cctcgaactc gtgtcacatt
    14821 ttcgtctgat gattttgact atctcttttt aatttattta acgactgata actccttctt
    14881 gagtgggtac tggacaagtc atcggcaaca acaactttac catttaatca ccaaagatcc
    14941 cgattatcat ttactaatcc atcgtcttca ggcactagtt ggaaaatatt ccgatattca
    15001 tgagcatatc cctaacttaa ttcctttttt caaaagaact ctctacaatt tacaaacttt
    15061 aattaccttt gatggttatt attttgatca atatcaaggc aacatgctac tactagataa
    15121 gcttgaaacc gttatcaaag attggcttta tgacacaggc cgtcaaggca gtattagttc
    15181 tggtcactta cacttgatgt gtttatacct tgagcagatc ttggaaagct ctattgctcc
    15241 aatcaatatt actgttattg agagccaaga aactgttggt aacgttatag gtaatttcat
    15301 cacctcaact atcccttctt acaaagttga actgtcacgg gtcaatatct tatctgataa
    15361 tatttatccc tatgataaac ctgtagatct ggttgtgacc agtcagaagc tcttgccttt
    15421 cttaaaagaa ctcggtgttt ttcccaaaga gacccgcttg tttggtttga gtttggactg
    15481 tatccaacag cagcgcgaag atttaatcaa aaccatctta gccttgcacc agaaccacta
    15541 ccaaaaacgt ttagaagaac tttggagagt tccttcttaa ttttttgaca gtccctccta
    15601 gatactttta cagtctaggg gggtctttaa ggttgtcact tgcaaccttg ataagctctc
    15661 tttttaacca ttgctatttg cttagaaaca gaactggaac tgttttagcg gttcaactcc
    15721 tcttgtagga tcttcttccc ttttttgaag gttttatctg aaatttggca taataaagtg
    15781 cgctcccatg ttctggaagc gcactttatt atcgctttta cttaaccttg tatccacttg
    15841 atgatggaag tgccgcccca attcttagat tgcttggcta acttttaagc tcttgtacaa
    15901 ggaagaagtc tttttgacta tctgttgatt agaggcgagc attaagctct gctgataatg
    15961 cttcaaaacc tggttttcca agaagcgcaa acatattacg tttgtaagct tcaacacctg
    16021 gttgatcaaa tgggttaaca gcattcatgt agcctgaaac agcgatggca agctcaaaga
    16081 agtaaatagt gtaaccaagg gtaaactcgt cttgagctgg aagagtgaca aacatgtttg
    16141 gcacgccacc atctgtgtgg gcaagaagca caccatctgt ggctttctta ttaacaaaat
    16201 caacgtcttt accttgaagg taaccaagac catctaggtc ttctgctaat tcaggaatga
    16261 taacattttt acgagggtta tcaacacgaa taactgtttc aaagaggtta cggtaacctt
    16321 cttggataaa ttgaccaagt gagtgaaggt ctgttgagaa gttagctgaa gttgggtaaa
    16381 tccctttttg atcttttcct tctgattcac cagctaattg tttccaccat tcgccaaagt
    16441 actgaagaga tggctcgtag ttagccaaga tttcagtgat gtagccttta cggtaaagca
    16501 cattacgaac agcagcgtat tggtaagcaa tgttttcaga gattttgtct gaagaaaggt
    16561 ctttacgagc agcatttgcc ccttccataa gagcagtaat atctgcacct gatgcagcaa
    16621 ttggaagcaa accaacagca gtcaagactg agaaacgacc accaacatta tctggcacca
    16681 caaatgtttc ccagttgtta gcgtcagctt ctaacttaac agcaccctta actttgtctg
    16741 tggtagcgta gatacgtttg ttagcttctt cttggccata ttttttaacc aaaagttctt
    16801 tgaacacgcg aaaagcaata gctggctcag tggttgtacc tgatttagag atcacgttta
    16861 ctgagaattc tttgtcttga acgtactcta caaggtcagc taagtaagtt gatgaaatag
    16921 agtttccagc gtaaagaatt tgtggggctt tgcgctcttt ggcagtttgc aagttagcaa
    16981 aatgatgatt taaaaaatca atcgctgctt tggcaccaag gtatgaccca ccgataccga
    17041 tcaccacaag aacttcgctg tctgccttaa tcttttcagc agcagtcaag atacgtgcaa
    17101 attcctcttt gtcatagttt tctggaagat caagccagcc caagaaatct gaacctggtc
    17161 cagttccttc acgcaataac ttgtcagctt ctgtgacttg gccttgtaaa aaatcaattt
    17221 catgttgtcc cgcaaatgac tcgaggactt ttgaataatc aaatgtaata tgtgacatga
    17281 gtttcgctcc cttaaaattt taagtttcat tagtatgata accttttcta tctaatattt
    17341 caatggtttt tagtggtttt cacctgtgaa aaagctaggt tttttgaaaa ggttttcgtt
    17401 ttttcagaca taaaaaatgt gtcaatgcaa gcatattctc atcatagtgg ttttttaaga
    17461 gaaaatagga tttcatattt cctaaaaata tcatgaaaaa aggccctgag gccgctttac
    17521 cgtaataact tattttaagg attttatcat ttcaacacgg gtaataaccc catcatgcat
    17581 atgatcaatg cataaagcca cacctacaaa ggcaattgaa gccccaatca cctgtaaata
    17641 ctttctcatg ctatcttctt aacattactt actctcatta tctaaaaatg gcaatcattt
    17701 gaatacttaa aaagcaaagg ggggagaata gcactagtgc gtttttaagt agatat
//
LOCUS       DATXIG010000033        17285 bp    DNA     linear   BCT 28-DEC-2023
DEFINITION  TPA_asm: Streptococcus pyogenes strain MGAS11113
            SAMN08555799-rid19682583.denovo.033, whole genome shotgun sequence.
ACCESSION   DATXIG010000033 DATXIG010000000
VERSION     DATXIG010000033.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN08555799
            Sequence Read Archive: SRR6740495
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pyogenes
  ORGANISM  Streptococcus pyogenes
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 17285)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 17285)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-OCT-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-OCT-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001918058.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001918058.2
            Genome Coverage       :: 368x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/12/2023 11:23:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 1,806
            CDSs (total)                      :: 1,769
            Genes (coding)                    :: 1,722
            CDSs (with protein)               :: 1,722
            Genes (RNA)                       :: 37
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1, 1 (5S, 23S)
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 47
            CDSs (without protein)            :: 47
            Pseudo Genes (ambiguous residues) :: 0 of 47
            Pseudo Genes (frameshifted)       :: 30 of 47
            Pseudo Genes (incomplete)         :: 20 of 47
            Pseudo Genes (internal stop)      :: 9 of 47
            Pseudo Genes (multiple problems)  :: 11 of 47
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..17285
                     /organism="Streptococcus pyogenes"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN08555799-rid19682583.denovo.033"
                     /strain="MGAS11113"
                     /serotype="Emm28"
                     /isolation_source="normally sterile site"
                     /host="Homo sapiens"
                     /db_xref="taxon:1314"
                     /country="Finland"
                     /collection_date="2002"
                     /collected_by="National Public Health Laboratory"
     gene            complement(123..695)
                     /gene="grpE"
                     /locus_tag="VC122_001144"
     CDS             complement(123..695)
                     /gene="grpE"
                     /locus_tag="VC122_001144"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002988583.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotide exchange factor GrpE"
                     /protein_id="HEP5641070.1"
                     /translation="MAVFNKLFKRRHSVSEEIKKDDLQEEVEATETEETVEEVIEETP
                     EKSELELANERADEFENKYLRAHAEMQNIQRRSSEERQQLQRYRSQDLAKAILPSLDN
                     LERALAVEGLTDDVKKGLEMTRDSLIQALKEEGVEEVEVDSFDHNFHMAVQTLPADDE
                     HPADSIAEVFQKGYKLHERLLRPAMVVVYN"
     gene            complement(698..1732)
                     /gene="hrcA"
                     /locus_tag="VC122_001145"
     CDS             complement(698..1732)
                     /gene="hrcA"
                     /locus_tag="VC122_001145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001872861.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heat-inducible transcriptional repressor HrcA"
                     /protein_id="HEP5641071.1"
                     /translation="MITQRQNDILNLIVELFTQTHEPVGSKALQRTIDSSSATIRNDM
                     AKLEKLGLLEKAHTSSGRMPSPAGFKYFVEHSLRLDSIDEQDIYHVIKTFDFEAFKLE
                     DMLQKASHILAEMTGYTSVILDVEPARQRLTGFDVVQLSNHDALAVMTLDESKPVTVQ
                     FAIPRNFLTRDLIAFKAIVEERLLDSSVIDIHYKLRTEIPQIVQKYFVTTDNVLQLFD
                     YVFSELFLETVFVAGKVNSLTYSDLSTYQFLDNEQQVAISLRQSLKEGEMASVQVADS
                     QEAALADVSVLTHKFLIPYRGFGLLSLIGPIDMDYRRSVSLVNIIGKVLAAKLGDYYR
                     YLNSNHYEVH"
     gene            complement(1867..2436)
                     /locus_tag="VC122_001146"
     CDS             complement(1867..2436)
                     /locus_tag="VC122_001146"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002983307.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 73 protein"
                     /protein_id="HEP5641072.1"
                     /translation="MRKRLKFPYFLTLLACFLLLIVCPLLSSQRIASADKEVRVNYSQ
                     KQFITKMGKEVKPLAKYYGIRPSILIAQILLETHDGKTLLASKYHNLFSKKATPGQAA
                     ITLKSPKQTNQNVRYAIYKDDASAIRDYLRMLRQGKEVDKRLYRNLATEKGYKAPAKS
                     LQKYLHYTDKTYARRLIQVIESNDLTNYD"
     gene            complement(2433..3176)
                     /locus_tag="VC122_001147"
     CDS             complement(2433..3176)
                     /locus_tag="VC122_001147"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012560905.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanyl-D-alanine carboxypeptidase family
                     protein"
                     /protein_id="HEP5641073.1"
                     /translation="MKHNHFKLLLQTLVVLLFVGGIYLFIKPEKQATKTPSSQQASSQ
                     KVVKNSKESTGLPKVSPKDWELILVNRDHMTEELSPELVDINGISVDKRIEQATSDFL
                     AAAQAIDPQEHLISGYRSVAYQAELYQSYIKKEMANDPTLTQEAAEALVQTYLQPPGA
                     SEHHTGLAIDMSTVDTLNASDPSVSKAVQKIAPDYGFVLRFPEGKKTSTGVDYEDWHY
                     RYVGKASARYMAQHNLTLEEYIAALKEKR"
     gene            complement(3166..3873)
                     /locus_tag="VC122_001148"
     CDS             complement(3166..3873)
                     /locus_tag="VC122_001148"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011528909.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine phosphatase family protein"
                     /protein_id="HEP5641074.1"
                     /translation="MTKTRLYIARHGKTMFNTIGRAQGWSDTPLTKKGEEGIRELGLG
                     LKDATIPFKAAFSSDSGRTMQTIEIILRESENEFLPYTKDNRIREWCFGSLEGTYDSE
                     LFLGVLPRTKAFENRDNLRDVPYSELAESIVEVDTANWAEPWEVLRKRIWEGFEAIAL
                     SIQNAGGGNALVVSHGMTIGTFMWLIDPDREKQYIDNGSVTVVEFEDGQFTIKTIGDM
                     SYRYRGREIIEAMSDEA"
     gene            complement(4265..4504)
                     /locus_tag="VC122_001149"
     CDS             complement(4265..4504)
                     /locus_tag="VC122_001149"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002988565.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GlsB/YeaQ/YmgE family stress response membrane
                     protein"
                     /protein_id="HEP5641075.1"
                     /translation="MIWSLIVGAFIGMIAGKMTEKSQSMGCFTNIIAGLIGSAVGQRL
                     FGDWGIQLAGMAIFPSILGAAIVIVLVSAIFGKRY"
     gene            complement(4723..4938)
                     /locus_tag="VC122_001150"
     CDS             complement(4723..4938)
                     /locus_tag="VC122_001150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002991523.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEP5641076.1"
                     /translation="MIVADSNKLKYFVSEKKAVGLSQAAFLYVMLVTVYKKTIIYAKI
                     RLTGIALEPSGVSDVYLMNYHVCLHYY"
     gene            complement(4935..6374)
                     /gene="gatB"
                     /locus_tag="VC122_001151"
     CDS             complement(4935..6374)
                     /gene="gatB"
                     /locus_tag="VC122_001151"
                     /EC_number="6.3.5.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002946582.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Asp-tRNA(Asn)/Glu-tRNA(Gln) amidotransferase
                     subunit GatB"
                     /protein_id="HEP5641077.1"
                     /translation="MNFETIIGLEVHVELNTNSKIFSPSSAHFGEDPNANTNVIDWSF
                     PGVLPVMNKGVIDAGIKAALALNMDIHKEMHFDRKNYFYPDNPKAYQISQFDEPIGYN
                     GWIEIKLEDGSTKKIRIERAHLEEDAGKNTHGTDGYSYVDLNRQGVPLIEIVSEADMR
                     SPEEAYAYLTALKEIIQYTGISDVKMEEGSMRVDANISLRPYGQEQFGTKTELKNLNS
                     FSNVRKGLEFEVERQAKLLRSGGVIRQETRRYDEANKGTILMRVKEGAADYRYFPEPD
                     LPLYEIDDAWIDEMRAQLPQFPAQRRAKYEEELGLSAYDASQLTATKALSDFFETAVS
                     LGGDAKQVSNWLQGEVAQFLNAEGKTIEEIRLTPDNLVEMIAIIADGTISSKMAKKVF
                     VHLAKNGGSARAYVEKAGLVQISDPAVLVPIIHQVFADNEAAVADFKSGKRNADKAFT
                     GFLMKATKGQANPQVAQQLLAQELQKLRD"
     gene            complement(6374..7840)
                     /gene="gatA"
                     /locus_tag="VC122_001152"
     CDS             complement(6374..7840)
                     /gene="gatA"
                     /locus_tag="VC122_001152"
                     /EC_number="6.3.5.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011285681.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Asp-tRNA(Asn)/Glu-tRNA(Gln) amidotransferase
                     subunit GatA"
                     /protein_id="HEP5641078.1"
                     /translation="MSFNHKTIEELHDLLVAKEISATELTQKTLEDIKSREEAVGSFI
                     TVSEEAALKQAAAIDAKGIDADNLMSGIPLAVKDNISTKGILTTAASKMLYNYEPIFD
                     ATSVANAYAKDMIVIGKTNMDEFAMGGSTETSYFKKTKNAWDHTKVPGGSSGGSATAV
                     ASGQVRLSLGSDTGGSIRQPAAFNGVVGLKPTYGTVSRYGLIAFGSSLDQIGPFAPTV
                     KENAQLLNVIASSDVKDATSAPVRIADYTSKIGRDIKGMKIALPKEYLGEGIDPEIKE
                     TVLAAAKQFEALGATVEEVSLPHSKYGVAVYYIIASSEASSNLQRFDGIRYGFRADDA
                     KNLDEIYVNTRSQGFGDEVKRRIMLGTFSLSSGYYDAYFKKAGQVRTLIIEDFDKVFA
                     DYDLILGPTTPTVAFGLDTLNHDPVAMYLADLLTIPVNLAGLPGISIPAGFVDGLPVG
                     LQLIGPKYAEETIYQAAAAFEAVTDYHKQQPIIFGGDK"
     gene            complement(7840..8142)
                     /gene="gatC"
                     /locus_tag="VC122_001153"
     CDS             complement(7840..8142)
                     /gene="gatC"
                     /locus_tag="VC122_001153"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008086915.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Asp-tRNA(Asn)/Glu-tRNA(Gln) amidotransferase
                     subunit GatC"
                     /protein_id="HEP5641079.1"
                     /translation="MKISEEEVRHVAKLSKLSFLESETTTFATTLSKIVDMVELLNEV
                     DTEGVAITTTMADKKNVMRQDIAEEGTDRALLFKNVPEKENHFIKVPAILDDGGDA"
     gene            complement(8374..8692)
                     /locus_tag="VC122_001154"
                     /pseudo
     CDS             complement(8374..8692)
                     /locus_tag="VC122_001154"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002991537.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate dikinase"
     gene            complement(9136..9690)
                     /locus_tag="VC122_001155"
     CDS             complement(9136..9690)
                     /locus_tag="VC122_001155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002988559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine hydrolase"
                     /protein_id="HEP5641080.1"
                     /translation="MRALISIDYTNDFVADDGKLSAGKSAQAIATKIAEVTKTAFDQG
                     DYIFFAIDCHDQNDPWHPESKLFAAHNIKGTTGRHLYGPLAEVYSYMKQHPRVFWIDK
                     RYYSAFSGTDLDIRLRERGITQLVLTGVLSDICVLHTAIDAYHLGYQLEIVKSAVASL
                     TKESYEWSLAHFEQVLGAKLINDY"
     gene            complement(9837..10619)
                     /gene="codY"
                     /locus_tag="VC122_001156"
     CDS             complement(9837..10619)
                     /gene="codY"
                     /locus_tag="VC122_001156"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001133183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GTP-sensing pleiotropic transcriptional
                     regulator CodY"
                     /protein_id="HEP5641081.1"
                     /translation="MPNLLEKTRKITSILQRSVDSLETELPYNTMASRLADIIDCNAC
                     IINGGGTLLGYAMKYKTNTDRVEEFFEAKQFPDTYVKAASRVYDTEANLSVENELTIF
                     PVESKDTYPGGLTTIAPIYGGGMRLGSLIIWRNDNEFSDDDLILVEISSTVVGIQLLN
                     LQTENLEDTIRKQTAVNMAINTLSYSEMKAVAAILGELDGNEGRLTASIIADRIGITR
                     SVIVNALRKLESAGIIESRSLGMKGTYLKVINEGIFAKLKEF"
     gene            complement(10837..12051)
                     /locus_tag="VC122_001157"
     CDS             complement(10837..12051)
                     /locus_tag="VC122_001157"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012679061.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal phosphate-dependent aminotransferase"
                     /protein_id="HEP5641082.1"
                     /translation="MKIIEKSSKLEHVAYDIRGPVLDEANRMIASGEKILRLNTGNPA
                     AFGFEAPDEVIRDLIVNARLSEGYSDSKGIFSARKAIMQYCQLKGFPDVDIEDIYLGN
                     GVSELISISLQALLDNGDEVLVPMPDYPLWTACVSLGGGKAVHYLCDEEAGWYPDIAD
                     IKSKITSRTKAIVVINPNNPTGALYPKEILEDIVALAREHQLIIFADEIYDRLVMDGK
                     EHIAIASLAPDVFCVSMNGLSKSHRIAGFRVGWMVLSGPKDHVKGYIEGLNMLANMRL
                     CSNVLAQQVVQTSLGGHQSVDALLLPGGRIFEQRNFIYEAINAIPGLSAVKPEAGLYL
                     FPKIDRQMYRIDDDEEFVLQLLKQEKVMLVHGRGFNWKDPDHFRIVYLPSVEELEDVQ
                     EKITRVLYQYRR"
     gene            12282..12734
                     /locus_tag="VC122_001158"
     CDS             12282..12734
                     /locus_tag="VC122_001158"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015055992.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein"
                     /protein_id="HEP5641083.1"
                     /translation="MSLKYKRILVAIDGSYESELAFNKGVNVALRNDATLLLVHVIDT
                     RALQSVATFDTYIYEKLEQEAKDVLDDFEKQAQIAGITNIKQIIEFGNPKNLLAHDIP
                     DRENADLIMVGATGLNTFERLLIGSSSEYIMRHAKIDLLVVRDSTKTL"
     gene            complement(12857..14245)
                     /locus_tag="VC122_001159"
     CDS             complement(12857..14245)
                     /locus_tag="VC122_001159"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011888631.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Cof-type HAD-IIB family hydrolase"
                     /protein_id="HEP5641084.1"
                     /translation="MTVKAVFFDIDGTLLNDRKNIQKTTQKAIQQLKKQGIMVGLATG
                     RGPGFVQPFLENFGLDFAVTYNGQYILTRDKVLYQNQLPKSMIYKVIRYANEKKREIS
                     LGTASGLAGSRIIDMGTSPFGQVISSFVPKSWARTVEGSFKHLIRRIKPQSFRNLVTI
                     MREPIYQVVLVASQAETKKIQEKFPHIKITRSSPYSLDLISVDQSKIKGIERLGEMFG
                     FDLSEVMAFGDSDNDLEMLSGVGIGIAMGNAETVVKDGAHFTTDSNNNDGISKALAHY
                     GLIHFDIEKSFKSRDENFNKVKDFHRLMDSDTIETPRSYTISEAGYRSGFKVEEIVEF
                     LYAASKGDQQQFTQAIFDLHGAVDQAANKVQAKKHVETPLIGQVDALADLLYFTYGSF
                     VLMGVDPQPIFEAVHEANMAKIFPDGKAHFDPVTHKIQKPDYWQERHAPEVAIKKELD
                     KQLQKSLQRFDK"
     gene            14317..15282
                     /locus_tag="VC122_001160"
     CDS             14317..15282
                     /locus_tag="VC122_001160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017643115.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="asparaginase"
                     /protein_id="HEP5641085.1"
                     /translation="MKKILVLHTGGTISMQADNSGRVVPNQDNPMTKIHAAAQDIQLT
                     VSDFLNLPSPHITPHHMLSIYHHIQERTDVFDGIVITHGTDTLEETAYFLDTMALPTN
                     IPVVLTGAMRSSNEVGSDGIYNYLTALRVASSDKAKEKGVLVVMNDEIHAAKYVTKTH
                     TTNISTFQTPTHGPLGIIMKNDLLFFKTAEPRIRFDLRCISGTIPIIKAYAGMGDGSI
                     LSLLTPGSIQGLVIEALGAGNVPPLAVGEIEHLITLGIPVVLVSRCFNGMAEPVYAYE
                     GGGAMLQEAGVMFVKELNAPKARLKLLIALNAGLTGQELKDYIEG"
     gene            complement(15631..16335)
                     /locus_tag="VC122_001161"
     CDS             complement(15631..16335)
                     /locus_tag="VC122_001161"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014635661.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEP5641086.1"
                     /translation="MISYLKADLFRIRQERLALFSCMLLVVIAVGVAYSFRKTPNQVI
                     MIQIINIITDFVTLFFLTPARIFFSDDYNYRTINNVVVKQQNRTLIIIYKLLVSFGLS
                     LLFISLTYLLSALAFHLFTGQAYYGIVIDRMIKQLPFYIAIIALCQFLFNILRKTYQS
                     ITAYILYVLLFDKVSATLANHFFKLDISPVLMFKHLSLSLGDKHLSSTSILVAFGLTL
                     CYLLGNVFLFSKRELK"
     gene            complement(16348..17250)
                     /locus_tag="VC122_001162"
     CDS             complement(16348..17250)
                     /locus_tag="VC122_001162"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009880760.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEP5641087.1"
                     /translation="MTQQSMLLSIEQVNKSYGKNQVLSDISFDIYKGEICGLVGQNGA
                     GKTTLMRILSGLIGKDSGQIKQLQPYRMGSIIESPTLYPNMTAHDNLYYAALQLRLAD
                     AKERIHEVLELIGLEKVSKKKKVKDYSLGMRQRLAIGLSILDFPEFLILDEPINGLDP
                     AGIKEMRQIILNLRDCYGITILISSHILSELDLVVDRYVIMHKGKVIKSMDKAELKAQ
                     VKVQIALHTSDDQLVKNRLLELGLRVETDGQMLLIKPTLSVMELIKIVLDLPVEIFDI
                     YHHQVSFEHYYLDLLGKDAASPLI"
BASE COUNT         5364 a         3643 c         2950 g         5328 t
ORIGIN      
        1 gcactcgaat tattttactt ccaattctaa gcaatcacta tagtttaatc agcaagcgaa
       61 ataacttcta acttgctatc tagtttactg tcattacgga caaaaacgaa aactcattta
      121 ccttagttat agacaactac catcgctggt cttagcagac gctcgtgcaa tttatatccc
      181 ttttgaaaga cttctgcaat gctatcggct ggatgttcat catcggctgg gagggtctga
      241 actgccatat gaaagttgtg gtcaaatgag tcaacttcta cttcttctac gccttcttcc
      301 tttagtgctt gaataaggct atcacgagtc atttctaaac cttttttaac atcatctgtc
      361 aacccttcaa cagccaaggc acgttctaga ttgtcaagcg atggtaagat tgcttttgcc
      421 agatcctgag aacgatagcg ttgaagttgt tgacgttctt cactagagcg acgctgtata
      481 ttctgcattt ctgcatgagc tcgcaagtat ttattttcaa actcatcagc acgttcgttt
      541 gcaagttcta actctgattt ttcaggagtt tcttcaataa cttcctcaac tgtctcttca
      601 gtttcagtgg cttccacttc ttcttgtaaa tcgtcttttt taatttcttc tgacactgag
      661 tgcctccttt taaataactt attgaatact gccattatca gtggacttca taatggttag
      721 aatttaggta tcgataataa tcacctaatt tagcagccaa tacctttcca ataatgttta
      781 ccaaactaac agacctacga taatccatat caataggacc aatcaaactc aggagtccaa
      841 acccacggta aggaatcaaa aatttgtgag tcaaaactga cacatctgca agtgcggctt
      901 cttgactatc tgccacttgg acagaagcca tctcaccttc tttcagactt tgcctcaaag
      961 aaattgctac ttgctgttca ttatccaaaa actgatacgt tgatagatct gaatacgtta
     1021 aagaatttac cttaccagca acaaataccg tttccaaaaa cagctctgaa aaaacataat
     1081 caaatagttg taaaacatta tccgtagtca caaaatactt ttgaacgatc tgaggaattt
     1141 ctgtcctcaa cttataatga atatctatga cactactatc cagcaagcgc tcctctacga
     1201 tagctttaaa tgctatcaag tcacgtgtca aaaagtttct tggaatagca aactgcactg
     1261 tcactggctt agattcatct aaagtcatga ctgctagagc atcatggttg ctaagctgaa
     1321 caacatcaaa ccctgttaaa cgttgacgag ctggctccac atctaaaata acggatgtat
     1381 agcccgtcat ctccgctaaa atgtgactag ctttttgaag catgtcttct aatttgaatg
     1441 cttcaaaatc aaaagttttt atgacatgat aaatatcttg ctcatcaata ctatctagcc
     1501 tgagggagtg ctctacaaaa tacttaaagc cagctggact tggcatccgt ccactagaag
     1561 tgtgcgcttt ttccaacaat cccaacttct ctagttttgc catatcatta cgaatagtgg
     1621 cgctactaga atcaatcgtt ctctgtaaag ctttagaccc tactggttca tgagtttgtg
     1681 taaatagctc aacgattaaa ttcaaaatat cattttgacg ttgtgtaatc acaaaccaac
     1741 cactcccttt cgtttagcac tctatttagt agactgctaa tttataattc tattatactc
     1801 tttatgggac acatgtcaag aaaaaaactc aaaaaaatta gcactctttt ataaagagtg
     1861 ctaatttcag tcatagtttg ttaaatcgtt actttcaatg acctgaatca gtcgcctagc
     1921 ataggtttta tcagtataat gcaaatactt ttgtaaactt ttagctggtg ctttataccc
     1981 tttttctgta gcaagattac gatacaaacg cttatcgact tcttttccct gccgaagcat
     2041 tcgtaaataa tctctaattg cactagcgtc atctttataa atagcatatc tcacgttttg
     2101 gttggtttgt ttaggggact ttagggtaat ggccgcttgt cctggagttg ctttcttgct
     2161 aaaaagatta tgatacttag acgctagtaa tgtttttcca tcatgggttt ccaaaagaat
     2221 ttgagcaatc aaaatagatg gtctaatgcc ataatatttt gccaatggtt taacttcttt
     2281 acccattttt gtaataaatt gtttttggct gtagttcacc cttacttctt tatctgcact
     2341 agctatcctt tggctactca gtaagggaca aacaatcagc aataaaaagc aagctagaag
     2401 cgttaaaaaa tacggaaact ttagtcgttt tctcatcgtt tctcctttaa agcagcaatg
     2461 tactcttcca acgttaggtt gtgctgagcc atataacgag cagacgcctt gcctacatag
     2521 cggtaatgcc aatcctcata atcaacccct gtactagtct tttttccttc tggaaagcgc
     2581 aagacaaacc cataatcagg tgcaattttt tgaactgctt tagataccga tgggtcactg
     2641 gcatttaaag tatctacagt gctcatgtca atagcaagac cagtgtgatg ttcactagcc
     2701 cctggcggct gtaaataggt ttgcaccaaa gcctccgcag cttcttgtgt caatgttgga
     2761 tcgttagcca tctctttttt aatataagat tggtaaagct ctgcttggta agctacggag
     2821 cgatagcctg aaataaggtg ctcttgtggg tcaatagcct gagctgccgc taaaaaatca
     2881 ctagtggctt gctcaatgcg tttatccaca gaaataccat taatgtcaac taattctggg
     2941 ctcaattcct cagtcatatg gtcccgattc actaaaatca attcccaatc ttttggagag
     3001 actttaggaa gccctgtgga ctctttgcta tttttgacca ctttttgact gcttgcctgt
     3061 tgacttgaag gcgtcttggt ggcctgcttc tctggcttaa taaagaggta aatacctccg
     3121 acaaagagca gaacaaccag tgtttgcaat aataatttaa aatgattatg cttcatctga
     3181 cattgcttct attatttcac ggccacggta gcgataactc atatctccaa tggttttgat
     3241 ggtaaattgc ccatcttcaa attcgacaac agtgacactg ccgttatcaa tatattgttt
     3301 ctcacggtca ggatcgatta gccacataaa tgttccaata gtcatcccat ggctaaccac
     3361 aagagcattt ccgccgccag cattttgaat agacaaagca atagcttcaa acccctccca
     3421 aatgcgctta cgaagaactt cccatggttc tgcccaatta gcggtatcta cttccacgat
     3481 gctttctgcc agttcagaat agggaacatc acgtaggtta tcacgatttt caaaggcttt
     3541 tgttcttgga aggacaccta gaaataattc tgaatcataa gtcccttcta gactcccaaa
     3601 acaccactca cgaatacgat tatctttagt ataaggcaaa aattcgtttt ctgattctct
     3661 taaaataatt tctattgtct gcatggttcg acctgaatca cttgaaaacg cagccttaaa
     3721 tggtatagtt gcatctttta aacctaagcc aagttctcga ataccttctt ctcctttttt
     3781 tgtaagaggt gtgtcactcc aaccctgagc gcgaccaatg gtattaaaca tcgtttttcc
     3841 atgtctggca atatacaacc ttgtttttgt catttcaacc tcctaaactg tcttattaat
     3901 tattatagca tttaaagaaa acacttgcaa aacacttcct gatagctatt tgagatcaaa
     3961 tagaccactg acttattgtc tattttatta aaaataatgc tattgctaca attcaaatca
     4021 tcacataaat ttccaaacga ttacatttgt gcgtttcgct caaatatata taacaacaga
     4081 cccacactac aactaaactg acattacaaa atgatgtcaa aacagtaaca aaatctaact
     4141 atttgtcaag aaaatgatat taaattttca cacaactatc ttataatgag gatgcaacca
     4201 ataaaaagat aagacatttt atgttgcaaa aaaggctgct tggaaacttc catgcagcct
     4261 ttttttaata cctttttcca aaaatagctg aaacaagtac aattacaatt gccgctccca
     4321 aaatggacgg gaaaatagcc atacctgcca actgaattcc ccaatcccca aaaagccttt
     4381 gcccaacagc tgaccctatg agacctgcga taatattagt aaaacaaccc atggactggc
     4441 ttttttcagt cattttacct gctatcatgc cgataaaggc acctacaatt aaagaccata
     4501 tcatcgttaa cacctccttt cttgtaaaat ccttttagaa gaactgctat ccattaaatg
     4561 ttcttaactt tagttgattc ctttaccttc ttctttagta aatgtttccg tgcctgagta
     4621 aaatagaaaa aacaattcct tctattaagg tagatagacc gagaacgact agaagtcttc
     4681 acacactaaa taacttaaca aactttatca cacagctaat cgctaataat agtgtagaca
     4741 aacgtgatag ttcattaaat acacgtcaga aactcctgaa ggttctaaag caatacctgt
     4801 cagtcttatt ttagcataga tgatggtctt tttatagacg gtcactaaca tgacatacaa
     4861 aaaggcagcc tgtgaaagac cgaccgcctt tttttcagag acaaaatact ttaacttatt
     4921 gctatcagca actatcaatc tcgcaatttt tgcaattctt gtgccaataa ttgttgagcg
     4981 acttgtggat tagcttgacc tttggttgcc ttcatcaaga agcctgtgaa agccttatca
     5041 gcattacgtt tgcctgattt gaaatcagcc actgcagctt cattatcagc aaagacttgg
     5101 tgaataattg gtaccaaaac agcgggatca gaaatctgaa caagtcctgc cttttcgacg
     5161 taggcgcgag ctgagccacc gtttttagcc aaatgcacaa agaccttctt agccatttta
     5221 gaagagattg taccatcagc aataatagca atcatttcca ctaagttgtc aggcgtcaat
     5281 ctaatttctt caatggtttt gccttctgca tttaagaatt gggcaacttc cccttgcaac
     5341 cagttagaga cttgcttggc atcgccacca agacttactg cagtttcaaa gaaatctgac
     5401 aaggctttgg tagctgttag ttggctagca tcataagctg agaggccaag ttcctcttcg
     5461 tatttagcac gacgctgagc tgggaattga ggtaattgag cacgcatctc gtcaatccaa
     5521 gcatcatcaa tctcatataa tggcaggtct ggttctggga aataacgata gtccgctgcc
     5581 ccttctttga cccgcatcaa aatagtcccc ttattcgctt cgtcatagcg gcgcgtttct
     5641 tggcgtatca caccacctga acgcaagagt ttggcttgac gttccacctc aaattcaagc
     5701 cctttacgga catttgagaa ggagttcaag tttttcaatt cggtctttgt tccaaattgt
     5761 tcttgcccgt aaggacgaag agaaatattg gcatccacac gcatggagcc ttcttccatc
     5821 ttgacatcag aaatacccgt atactggatg atttctttca gagctgtcaa ataagcatag
     5881 gcttcttcag gagaacgcat gtcggcttct gacacaatct caatcaaagg aactccttgg
     5941 cggttaaggt ccacataaga ataaccatct gtgccatgag tgtttttacc agcatcttct
     6001 tccaagtgtg cgcgttcaat acgaattttc ttagtcgagc cgtcttccaa tttaatctca
     6061 atccatccgt tgtagccaat cggctcgtca aactgtgaaa tttggtaagc tttaggattg
     6121 tctgggtaga aataattttt gcggtcgaaa tgcatctctt tatggatgtc catattgagg
     6181 gcaagcgcag cttttatgcc cgcatcaatc acccctttgt tcatgactgg taagacccct
     6241 gggaaagacc aatcaatcac attcgtgttg gcatttgggt cctcaccaaa gtgagctgac
     6301 gaaggtgaga aaattttgga attggtattt aattccacat ggacttcaag cccaataatg
     6361 gtttcaaaat tcattacttg tcacctccaa aaataatcgg ttgttgcttg tgataatctg
     6421 tcaccgcttc aaaggctgct gccgcctgat agatggtttc ttcagcgtat ttcggaccaa
     6481 ttaattgaag ccctactggc aagccatcga cgaatcctgc tggaatggaa attcctggca
     6541 gaccagccaa gttaactgga atggtcaata agtcggccaa atacatggca acaggatcgt
     6601 ggttgagtgt gtctaagcca aaagcaaccg tcggtgttgt tgggcctaaa ataagatcat
     6661 aatccgcaaa gaccttgtca aaatcttcaa taatcaaggt acgcacttga ccggcctttt
     6721 tgaaataagc gtcataataa cctgatgaca aactaaaggt cccaagcatg atacgacgtt
     6781 taacctcatc accaaagcct tggctacgag tgttaacata gatttcatcc aagtttttag
     6841 catcgtcagc acggaaacca tagcgaatgc catcaaaacg ttgtaagttt gaggacgctt
     6901 ctgacgaagc aataatgtaa tagacagcca caccatattt gctatgtgga agactaactt
     6961 cctcaacagt agctcctaag gcttcaaatt gtttagctgc cgcaagaacc gtttccttaa
     7021 tctcaggatc aatcccttcg ccaagatact ctttaggaag ggcaatcttc atgcctttaa
     7081 tgtcacgacc aatcttagat gtgtagtctg caatgcggac aggcgctgaa gtggcatcct
     7141 tgacatcgct acttgcaatc acatttaata attgggcgtt ttctttaact gtcggagcaa
     7201 aaggtccaat ctgatcaagt gaacttccaa aagcaatgag accatagcga gaaacagtgc
     7261 cataggttgg tttcaaaccg acaacaccat taaatgcagc tggctgccga atagaaccac
     7321 cagtgtctga accaagagat aaacggactt gtcctgaagc gactgctgtt gccgaaccac
     7381 ctgatgagcc accaggtact tttgtgtggt cccaagcatt tttcgttttc ttgaaataag
     7441 aggtttctgt tgaaccaccc atggcaaatt cgtccatgtt ggtttttcca atgacaatca
     7501 tgtctttagc gtaggcattt gctactgatg tggcatcaaa aatcggctcg tagttataca
     7561 acatttttga agcggctgtg gttaggatgc ccttggttga gatattatcc ttaacagcta
     7621 gtggaatacc cgacatgagg ttatcagcat caataccttt ggcatcaatt gccgcagctt
     7681 gtttaagagc agcttcttct gaaacagtga taaacgaccc gacagcctcc tcacgagact
     7741 tgatgtcttc aagtgttttt tgagtcaatt cggttgcgga aatctcctta gctacaagga
     7801 ggtcatgcaa ctcttcaatg gttttatgat taaaagacat taggcatctc ctccgtcatc
     7861 caaaatagca ggtaccttga taaagtgatt ttctttttca ggaacatttt taaagagcaa
     7921 ggcacggtct gttccttcct cagcaatatc ttgacgcatc acatttttct tatctgccat
     7981 ggttgttgta atggcgacac cttcggtgtc aacttcgttc agcaattcaa ccatatcaac
     8041 gattttggat aaggtcgttg caaaggttgt tgtctcactt tctaagaatg ataattttga
     8101 tagcttcgcg acatggcgaa cttcttcttc agaaattttc atgatttctc ccatccaaga
     8161 tgatcagaag tattccaatt ccaaagaaca gctctgctat tctccaaagg tagcggaaaa
     8221 actcctcaca tcgtttttac atagtctcaa ttattatatc acaattgcgc ccatttacca
     8281 taggtgacta atgcaattct agaaagctat acagcaaaaa acttcctgac taaggtcaga
     8341 aaaccttagt caggaagtgt gacaaactaa actctatttg tcttgggtaa gactgtggcc
     8401 tgaagctttg atggcagcct gagcagccgc caagcgagta agcggcactc taaatggcga
     8461 acaagaaacg taggtcaggc cttggctgtg gtaaaagtca atggaagcag gatcgccacc
     8521 atgttcccca caaataccaa gtttcaactc aggcttaacc gaacgggcta aagagacggc
     8581 caatcccttt ctgatcaaag gtctggaacg gctccttatc aaaaataccc ctgtcaacgt
     8641 attcttccaa gtattttcct acaacatcac agaaaaagta acgttctttc aagtcggtaa
     8701 tatgtttacg aaggtagtaa aggggtgctt tattctaatt taccaaatcc tcaacgattg
     8761 tgacaacatg tgatgtgata agcctaagat aaaaatggta cagactctag cattcaagag
     8821 tttgaagctt tatttatatt ctaatgctga aaagactaga gtttgtctaa tggctgtgag
     8881 acgatcctct aaaccaccaa ctcaaatcat ttctcaaaca gctaaaatcc tgtctccttc
     8941 aaaaaaacat gtgttctatc tgacacagtc caaagtttag ttgcctttta gacagcacaa
     9001 gaatcatcag gactaattct gaccagcatg tcacatacct catctaccta acccatacat
     9061 acggtaaaac cacatagagc aatgattacg gttaccaaac tcttagtcac tgaatcaata
     9121 gtttggtagg ctcttttagt aatcgttaat aagcttagca ccaagaacct gctcaaagtg
     9181 agccaaagac cactcataag attcttttgt aaggctagcg acagccgatt taacaatctc
     9241 taattgataa ccaagatgat aagcgtcaat agcagtatgt aagacacaga tatcagataa
     9301 aactcctgtt aacactaatt gagtgatacc tctttctcgt agtctaatat ctaaatcagt
     9361 ccccgaaaaa gctgaataat aacgcttatc aatccaaaaa actctgggat gttgcttcat
     9421 ataactgtaa acttcagcta gtggaccata aagatggcgt ccagtagttc ctttgatatt
     9481 atgtgctgca aataacttac tctcaggatg ccaaggatca ttttgatcat gacaatcaat
     9541 agcaaaaaaa atatagtctc cttgatcaaa tgccgtttta gttacctctg ctattttagt
     9601 ggcaatagcc tgagcagatt tacctgcact taatttacca tcatctgcta caaaatcatt
     9661 tgtgtaatca attgaaatca atgctctcat cactctctcc taaacaggga aacctctccc
     9721 cttatctctt atatatggca ttgtatcagt ttttattaca ggtatcaaga catctatttg
     9781 catctaccat taacagcaaa aaacatctct tttcaagaga tgttgtcttt ttctatttaa
     9841 aattctttta atttagcaaa aataccttcg ttgataactt tgaggtatgt ccctttcata
     9901 ccaagagaac gactttcaat aatccctgca ctttctaatt tacgcagagc attgacaata
     9961 acagaacggg taataccaat acgatcagca ataatagaag ctgtcaatcg tccttcgtta
    10021 ccatctaact ctccaagaat tgctgcaaca gctttcattt ctgaataaga aagcgtatta
    10081 attgccatgt tgaccgctgt ttgtttacgg atggtgtctt ctaagttttc tgtctgaaga
    10141 tttaatagtt gaatccccac aacagtactt gagatctcaa ccaaaatcaa atcatcatca
    10201 ctaaactcat tgtcattacg ccagataatg agtgatccaa ggcgcatccc tccaccataa
    10261 atcggcgcaa tagtcgttag acctcctgga taagtgtctt tagactcaac agggaatata
    10321 gtcaattcat tttcgacaga aaggttagct tctgtatcgt aaactcggct agcagccttt
    10381 acataagtat ctggaaattg tttagcttca aaaaattcct caacacgatc agtgttagtt
    10441 ttatatttca tggcataacc aagcagtgtg ccgccgccat tgataataca agcattgcag
    10501 tcaatgatat ctgctaggcg agatgccatt gtgttatacg gtaattctgt ttctaggcta
    10561 tctacggaac gctgcaaaat agatgtgatt ttacgagttt tttctaataa gttaggcatt
    10621 tttttccttt tcctttgaat tatttgcgct ttcattataa caaagctaac tcgcttttgc
    10681 aaggaattat cagaaaattt tctattctta ctacaattcc aactattgat gccttattat
    10741 cacttcattt aaaggagata agcactagct tgttagagca taggatcaca ttcttttaag
    10801 aaacgctatt tttattaatc aaagatgagc attgcattaa cgtctatact ggtataagac
    10861 acgtgtaatc ttttcttgga catcttctaa ttcttctaca cttggtaggt aaacaatacg
    10921 gaagtgatct ggatctttcc agttaaaacc acgtccatgt accaacatca ctttttcttg
    10981 ctttaataac tgtaagacaa attcttcatc atcatcaata cgatacatct gacgatcaat
    11041 tttcggaaat aaataaagac ctgcttcagg tttaactgcc gataagccag ggatagcatt
    11101 aatagcttca taaataaaat tacgttgctc aaaaatacgc cctcctggca gtaacagtgc
    11161 atcaactgat tgatgacctc ctaaggaagt ttgaacaact tgttgggcta aaacattgga
    11221 gcataaccgc atattggcaa gcatgtttaa cccttcaatg taacctttaa catggtcttt
    11281 aggacctgat aagaccatcc atccaacacg aaaaccagca atacgatgag atttggaaag
    11341 accattcata gatacacaaa aaacatcagg tgccaaactt gcaatagcaa tatgctcttt
    11401 accatccatc accaaacgat catagatttc atcggcaaaa ataatcagct ggtgttctct
    11461 tgccaaagca acgatgtctt ctaaaatctc cttaggataa agggctcctg ttgggttatt
    11521 aggattgatg accacaatag ctttggtgcg actcgtgatt tttgatttga tatcagcaat
    11581 atcaggatac caaccagctt cttcatcaca aagataatgg accgcttttc ctccaccaag
    11641 actcacacaa gccgtccata aaggataatc cggcattggt actaaaactt catcgccatt
    11701 atctagcaaa gcttgaaggg atatggatat caactctgac acgccatttc ccaaataaat
    11761 atcttcaatg tcaacatctg ggaagccctt gagttggcaa tactgcatga tagccttacg
    11821 cgctgaaaaa atccctttac tgtcagaata gccctcactt agcctcgcgt tgacaattaa
    11881 atcacgaatc acttcatctg gtgcttcaaa accaaaagca gcaggattgc cagtatttaa
    11941 acgcagaatt ttttcaccac tcgcaatcat gcgattagct tcatccaaaa caggccctcg
    12001 aatatcatag gcaacatgtt ctaattttga tgatttttca ataattttca tagtctcata
    12061 cacctcgtat tccatattat aaaccaaaat acttaaaata gaaagaattt atccatcaaa
    12121 gcttaggaca gctaatcgtt ttaacacagg tcttatgaga cgataataca gtctttagtt
    12181 tagcaaaaat aatggatcgc tttcaaaatg actcaaaaaa gagtataatg aaaaaaagaa
    12241 taaaagctat tcttgtcaaa ctgttaaagg aggacagttc tatgtcacta aaatataaac
    12301 gtatcctcgt tgctattgat ggctcttatg agtctgaatt agctttcaat aaaggcgtta
    12361 acgtagcact acgtaacgac gctaccttac tactagtaca cgttattgat acccgtgcct
    12421 tgcaaagcgt agctactttt gatacttaca tctatgaaaa attagaacaa gaagctaagg
    12481 atgttttaga tgattttgaa aaacaagcac agatagcagg aattacaaac atcaaacaaa
    12541 ttattgaatt tggcaacccc aaaaacttac tagctcacga tattccagat cgtgagaacg
    12601 ctgatttaat tatggtagga gctactggtc ttaatacctt tgaacgcctc cttattggct
    12661 catcttctga gtacattatg cgacacgcta agattgactt acttgtagtc cgagatagca
    12721 ctaaaacact ctagtctatt acaattttca aacaccaaaa aacaagcagc atctacaacg
    12781 aaattgcttg tttttttgtg ctgagctaag tctagtcaat atctttttat ttcttgatat
    12841 ttaaaagagt aaacctctat ttatcgaatc gttgtagtga tttttgtaat tgtttgtcta
    12901 actctttttt tatggctact tcaggtgcgt gacgctcttg ccaatagtca ggtttttgaa
    12961 ttttatgggt aacggggtca aagtgtgcct tgccatctgg aaaaatttta gccatatttg
    13021 cttcatgtac tgcttcaaat ataggttgtg gatctactcc catcagtaca aaagaaccat
    13081 aagtaaaata aagcaaatcc gctaaggcat caacttgccc aatcaggggc gtttcaacat
    13141 gttttttagc ttgtacctta ttagctgctt ggtcaacagc accatgcaaa tcgaaaatag
    13201 cctgtgtaaa ttgttgttgg tctcccttgc ttgctgcgta aagaaactca acaatttctt
    13261 ctactttaaa gcctgaccta taaccagctt ctgaaatggt ataactacgc ggtgtttcaa
    13321 tagtatctga gtccatcaga cgatgaaaat ctttgacttt attaaaattt tcatcacggc
    13381 ttttaaagct cttctctata tcaaaatgga tgagaccgta gtgagctaag gctttggaaa
    13441 tcccatcatt attattagag tcggtagtga aatgggctcc atcttttacc acagtttcag
    13501 catttcccat agcaataccg ataccaactc ctgataacat ttcaaggtca ttatctgaat
    13561 ctccaaaagc catcacttca gacaagtcaa atccaaacat ctctcccaaa cgttcaattc
    13621 cttttatttt tgattgatca acagagatca gatccaagga ataaggacta cttcttgtaa
    13681 tttttatatg agggaacttc tcttgtattt tcttagtttc agcttgtgac gctaccagaa
    13741 caacctgata aataggctcc cgcataatag ttactaaatt tctaaaactc tgaggcttga
    13801 ttcggcgaat caaatgctta aaactaccct caactgttct tgcccaactt ttgggcacaa
    13861 aactactaat gacttgtcca aatgggctag tccccatatc aataatacgc gatcctgcta
    13921 atccagaagc tgttccaaga gaaatttctc gctttttttc gttggcatag cgaatcactt
    13981 tgtaaatcat cgactttggc aattgattct gatataagac cttatctctt gttaagatat
    14041 attgcccatt gtaagtaacg gcaaaatcta atccaaaatt ttctagaaaa ggttgtacaa
    14101 atcccggtcc acgaccagtt gctaaaccga ccataatgcc ttgttttttg agttgttgga
    14161 tggctttttg cgttgttttt tggatatttt tacgatcatt gagaagggtc ccatcaatat
    14221 caaaaaagac tgcttttact gtcaaaacct tcttttgcct cctgtgttta tgatagaata
    14281 gattatatca taaataaaag gaagtttgac aatcaaatga aaaaaatcct agtcttacac
    14341 actggtggaa ccatttctat gcaagctgat aactctggta gggttgttcc aaatcaagat
    14401 aatcctatga cgaaaataca tgctgcagcc caagatattc agcttaccgt tagtgatttt
    14461 ctaaatcttc cgagtcctca catcacgcct caccacatgt tatctattta tcatcatatt
    14521 caagaaagaa ctgatgtctt tgatggtata gtcatcacac atggaacaga taccttagaa
    14581 gaaacagctt attttttaga taccatggca ttgcctacta atatacctgt tgtcctaaca
    14641 ggtgctatga ggtcttctaa tgaagttggt agtgatggca tatataatta ccttaccgca
    14701 cttagagttg caagtagtga taaagctaaa gaaaaagggg tacttgttgt tatgaacgat
    14761 gaaatccatg ctgctaaata tgttactaaa acacatacaa ccaatatttc aactttccaa
    14821 acacccacac acggaccgct tggtatcatc atgaaaaatg acctactttt tttcaaaact
    14881 gctgaacctc gtattcgttt tgacttgaga tgcatttctg gaaccatccc cattatcaaa
    14941 gcatacgcag gtatgggcga tggtagtatc ttgagtctat taactccagg tagcattcaa
    15001 ggtctagtta tcgaagctct aggggcagga aatgtcccac cattagcagt tggggagatc
    15061 gagcacttaa ttactttagg tattcctgtc gttcttgttt cccgttgttt caatggtatg
    15121 gcagaacctg tctatgctta tgaaggtggt ggtgcgatgc tacaagaagc aggtgttatg
    15181 tttgtaaaag aattgaatgc tccaaaagcc cgtctaaagt tattaattgc tctcaacgcc
    15241 ggacttactg gtcaagagct aaaagattat attgaaggat aatctagctt cctttagaaa
    15301 tgtcccacta aaacatatta gtgggacatt tctagtactt taagctttaa attaggacat
    15361 ctcctaaatg tctagaataa acaactatgt ttcaaactat gagattgtgc actcataatc
    15421 atttctataa cagtttgaga ccagtattcc aataaatcca actcttagca atcagatacc
    15481 gggtaaacaa gtctttattt tgtctcatta agtatgagct tacttgaata ataacaaaac
    15541 tacttgctta tcctttttta gtaatgatga agacgacctt tttctaagaa cttctttgtc
    15601 ttttaaaata aacaaagcac atattaccct ttattttagt tctcgtttgg aaaaaagaaa
    15661 gacattgcct aaaagataac aaagagttaa accaaaggcc actaatatgc tagtactaga
    15721 aaggtgttta tcaccaagag acaaggatag gtgtttaaac atcaaaactg ggctaatatc
    15781 taacttaaaa aaatggtttg ctaaagtagc agatacttta tcaaaaagaa ggacataaag
    15841 tatataagct gtgattgact gataagtttt acgaagtata ttaaacaaaa attgacacaa
    15901 agcaataata gcaatataaa aaggcagttg tttaatcatc cgatctatga ctattccata
    15961 gtatgcttga ccagtaaata aatgaaatgc taaagcacta agaagataag ttaaactaat
    16021 aaataaaaga gataagccaa aactaactag cagtttatag attataatta aagtacgatt
    16081 ttgttgtttg accacaacat tattaatcgt acggtagtta tagtcatcac taaaaaagat
    16141 acgggctggt gttaaaaaga aaagcgtcac aaaatcagta ataatattaa tgatttgtat
    16201 catgatgact tggttaggtg tcttgcgaaa actataggct acaccaacag caatgacaac
    16261 aagtagcata caagaaaata gagctaaacg ctcttggcgt atccgaaata aatctgcttt
    16321 aagataggaa atcataaaaa cctctttcta aattaatggt gatgctgcat ctttacctaa
    16381 caaatcgagg taataatgtt caaagctcac ttgatgatga taaatgtcaa aaatctcaac
    16441 aggcagatct agaacaattt tgataagctc cattactgat aaagttggtt ttattagtag
    16501 catctgacca tcagtctcaa ctctaagccc caattctaaa agtctatttt ttaccagctg
    16561 atcatcgctt gtatgaaggg caatttgtac ttttacttga gctttaagct ctgctttgtc
    16621 catactctta ataactttgc ctttatgcat aatcacgtaa cgatcaacaa ctaagtctaa
    16681 ttctgataaa atatgactgg aaatcaaaat cgtaatacca tagcaatctc ttaggttcaa
    16741 aataatctga cgcatttctt tgattcctgc tggatcaaga ccattaatgg gttcatccaa
    16801 gattaaaaat tctggaaaat ccagtatcga aagaccaata gccaaacgtt ggcgcatccc
    16861 taaagaataa tctttcacct ttttcttttt gctcactttt tctaacccaa taagttccaa
    16921 aacctcatgt atgcgctctt ttgcatcagc taatctcaat tgcaacgctg catagtaaag
    16981 attatcatga gctgtcatat tgggataaag tgtcggtgac tcaataattg atcccattcg
    17041 atagggttgt aactgtttga tttgccctga atcttttcca attaggccag ataaaatacg
    17101 cattaacgtt gtctttccag caccattttg acccacaagg ccgcaaattt ctcctttgta
    17161 aatatcaaaa gagatatcac ttaaaacctg atttttacca taggatttat ttacttgctc
    17221 aatacttagt aacattgatt gctgtgtcat tttccgctcc ttttttgtac ttatcgatta
    17281 ataca
//
LOCUS       DATXIG010000034        15769 bp    DNA     linear   BCT 28-DEC-2023
DEFINITION  TPA_asm: Streptococcus pyogenes strain MGAS11113
            SAMN08555799-rid19682583.denovo.034, whole genome shotgun sequence.
ACCESSION   DATXIG010000034 DATXIG010000000
VERSION     DATXIG010000034.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN08555799
            Sequence Read Archive: SRR6740495
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pyogenes
  ORGANISM  Streptococcus pyogenes
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 15769)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 15769)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-OCT-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-OCT-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001918058.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001918058.2
            Genome Coverage       :: 368x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/12/2023 11:23:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 1,806
            CDSs (total)                      :: 1,769
            Genes (coding)                    :: 1,722
            CDSs (with protein)               :: 1,722
            Genes (RNA)                       :: 37
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1, 1 (5S, 23S)
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 47
            CDSs (without protein)            :: 47
            Pseudo Genes (ambiguous residues) :: 0 of 47
            Pseudo Genes (frameshifted)       :: 30 of 47
            Pseudo Genes (incomplete)         :: 20 of 47
            Pseudo Genes (internal stop)      :: 9 of 47
            Pseudo Genes (multiple problems)  :: 11 of 47
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15769
                     /organism="Streptococcus pyogenes"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN08555799-rid19682583.denovo.034"
                     /strain="MGAS11113"
                     /serotype="Emm28"
                     /isolation_source="normally sterile site"
                     /host="Homo sapiens"
                     /db_xref="taxon:1314"
                     /country="Finland"
                     /collection_date="2002"
                     /collected_by="National Public Health Laboratory"
     gene            <1..346
                     /locus_tag="VC122_001163"
     CDS             <1..346
                     /locus_tag="VC122_001163"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014411839.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="LPXTG cell wall anchor domain-containing
                     protein"
                     /protein_id="HEP5641088.1"
                     /translation="LPGKDGKDGQPGKPAPKTPEVPQKPDTAPHTPKTPQIPGQSKDV
                     TPAPQNPSNRGLNKPQTQGGNQLAKTPAAHDTHRQLPATGETTNPFFTAAAVAIMTTA
                     GVVAVAKRQENN"
     gene            532..969
                     /gene="msrB"
                     /locus_tag="VC122_001164"
     CDS             532..969
                     /gene="msrB"
                     /locus_tag="VC122_001164"
                     /EC_number="1.8.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014407546.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide-methionine (R)-S-oxide reductase MsrB"
                     /protein_id="HEP5641089.1"
                     /translation="METSDELKQRIGDLSYEVTQHAATESPFTGEYDNFFEKGIYVDI
                     VSGEVLFSSLDKFNSGCGWPAFSKPIENRMVTNHDDSSYGMRRVEVKSREAGSHLGHV
                     FSDGPKEAGGLRYCINSAALKFIPYEQMEKEGYAQWLTLFDET"
     gene            1099..2118
                     /locus_tag="VC122_001165"
     CDS             1099..2118
                     /locus_tag="VC122_001165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015017089.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YeiH family putative sulfate export transporter"
                     /protein_id="HEP5641090.1"
                     /translation="MSTHLRKLPGLLLCLLLALPAWYLGRLFPIIGAPVFAILLGMLL
                     ALFYHHRDKAKEGISFTSKYILQTAVVLLGFGLNLTQVMAVGMQSLPIIISTIATALL
                     VAYGSQKWLRLDVNTATLVGVGSSICGGSAIAATAPVIKAKDDEVAKAISVIFLFNML
                     AALLFPSLGQLLGLSNDGFATFAGTAVNDTSSVTATATAWDALHHSNTLDDATIVKLT
                     RTLAILPITLGLSLYRAKKEHDIVTEENFSLRKSFPRFILIFLLASLITTLMTSFGVS
                     ADVFHSLKTLSKFFIVMAMAAIGLNTNLVKLIKTGGQAILLGAICWVAITLVSLAMQL
                     SLGIW"
     gene            2325..2750
                     /locus_tag="VC122_001166"
     CDS             2325..2750
                     /locus_tag="VC122_001166"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000823118.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HEP5641091.1"
                     /translation="MKRKFLIGSHGRLASGLQSSIDILAGMGQALETIDAYVDDSDYT
                     SQIDNFIAGVAADEQGLIFTDLLGGSVNQKMVTAVMNSGKDNIFLITNSNLATLLSLV
                     FLKPGEALTKDDIVTVINESQVQLVDLVPETNSEDDFFD"
     gene            2769..3260
                     /locus_tag="VC122_001167"
     CDS             2769..3260
                     /locus_tag="VC122_001167"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003051135.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS system
                     mannose/fructose/N-acetylgalactosamine-transporter subunit
                     IIB"
                     /protein_id="HEP5641092.1"
                     /translation="MITQIRVDDRLIHGQVAVVWTKELNAPLLVVANDEAAKNEITQM
                     TLKMAVPNGMKLLIRSVEDSIKLFNDPRAKDKRIFVIVNSVKDACAIAKEVPDLEAVN
                     VANVGRFDKSDPASKVKVTPSLLLNPEEMAAAKELVSLPELDVFNQVLPSNTKVHLSQ
                     LVN"
     gene            3277..4086
                     /locus_tag="VC122_001168"
     CDS             3277..4086
                     /locus_tag="VC122_001168"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017644846.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS
                     mannose/fructose/sorbose/N-acetylgalactosamine transporter
                     subunit IIC"
                     /protein_id="HEP5641093.1"
                     /translation="MLVPATMAALAVLICFGGNYLTGQSMMERPLVVGLVTGLLLGDM
                     KVGILMGASLEALFLGNVNIGGVIAAEPVTATAMATTFTIISHIDQKAAMTLAVPIGM
                     LAAFVVMFLKNVFMNIFAPMVDKAAAANHQGKLVMLHYGTWIIYYLIIASISFIGILV
                     GSGPVNAFVEHIPQNLMNGLSAAGGLLPAVGFAMLMKLLWTNKLAVFYLLGFVLTAYL
                     KLPAVAVAALGAVICVISSQRDLELDAITRGAISKQTTFDSKESEEEDFFA"
     gene            4083..4910
                     /locus_tag="VC122_001169"
     CDS             4083..4910
                     /locus_tag="VC122_001169"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000841956.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS mannose/fructose/sorbose transporter family
                     subunit IID"
                     /protein_id="HEP5641094.1"
                     /translation="MTSQDNLTKEDRKMLRSVFWRSWTMNASRTGATQYHAVGVIYTL
                     LPVINRFYKTDKDKAEALVRHTTWFNATMHINNFIMGLVASMEKKNSEDPDFDASAIT
                     AVKASLMGPISGVGDSFFWGILRVIAAGIGISLASAGSAMGAVVFLLLYNIPAFIIHY
                     YSLYGGYSVGAGFIKKLYESGGIKIVTKTSSMLGLMMVGSMTASNVKFKTILTVAAKG
                     AKEAASIQDYLDQLFIGIVPLMVTLAAFWLLRKKVNINWIMFGIMFLGIILGLLGIC"
     gene            5046..6695
                     /locus_tag="VC122_001170"
     CDS             5046..6695
                     /locus_tag="VC122_001170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014407549.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sensor histidine kinase"
                     /protein_id="HEP5641095.1"
                     /translation="MRGEQVEEHFKKQLQDDISRHFSYQSLMLSLLLIGLFIIFSLAP
                     QQLGLYRDINATATRYHRLISKQEALLDDLGKNSLLPFLNKNLSTADLSKHYFHLRHS
                     SQTSPELLLFSPSQDLLFASNPHLGNVFSKSVYIQEVLRAAHPPKTLFKVAMDSEDGH
                     YLMIIKPMIDQNQLKGYAFLVMSGKDFLHPTKTLTSELVIADKLDNTFTFSNREFIAS
                     SLDKINSQYLHHYFVFQDNRAFITRKVALQGGLWLYMYRPLIPMVSVMLFSLISSAVI
                     FVILQRKSSGLANRIAAKNSRAINQMVRDMSAISRQEKRRIDLESQDEFQYLSDQINQ
                     MVERLQQLHDKTLDLETQKLLFEKRMLEAQFNPHFLYNTLETILITSHYDSALTEKIV
                     IQLTKLLRYSLTDSSKPVLLKDDLSVIESYLVINQVRFEELQYSINLSPDLDSLAVPK
                     LFLLPLIENAIKYGLKERHDVKINIACYYQDDHIIFSVRDNGSGIDAHHQKVIREQLE
                     AGESHHGLINSYRRLKYHFSEVSLVFDQGDKQFNVSYHVKE"
     gene            6699..7487
                     /locus_tag="VC122_001171"
     CDS             6699..7487
                     /locus_tag="VC122_001171"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011017727.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HEP5641096.1"
                     /translation="MYKLVIIEDEHIIRKWLRYAIDYKALDILVIGEAKDGKEGAVLI
                     KESQPDIVLTDINMPIMTAFDMFEATKDQTYAKIILSGYADFPNARSAIHYGVLEFLT
                     KPIEKAALWECLQTIIAKIEKQKGSNQKTDACVYIPLPQMTDQIPEVVKDILEWVHAH
                     FQDKISTSRLAHDLGYSESYIYQNIKKHLQMPLSDYINQYRINQAIQLMQQEPDLMVY
                     EIAQAVGIYDYRYFDRVFKKYLGQTVKAFKEEHFMKDTDRSKPC"
     gene            7481..8527
                     /locus_tag="VC122_001172"
     CDS             7481..8527
                     /locus_tag="VC122_001172"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014407551.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="extracellular solute-binding protein"
                     /protein_id="HEP5641097.1"
                     /translation="MLKLKRKWLLSFLLVIIILAIVNIAMYIFSSSKKDSAKELVILT
                     PNSQTILTGTIPAFEEKYGVKVRLIQGGTGQLIDQLGRKDKPLNADIFFGGNYTQFES
                     HKDLFESYVSPQVSTVISDYQLPSHRATPYTINGSVLIVNNELARGLHITSYEDLLQP
                     ALKGKIAFADPNSSSSAFSQLTNILLAKGGYTSSEAWEYVKRLLLSMNSIRATSSSDV
                     YESVAEGKMIVGLTYEDPCINLQKSGANVSIVYPDEGTVFVPSSVAIIKKAKAMKEAK
                     QFINFILSSDVQNAFGQSTSNRPIRRDAQTSHEMKPLDEIITLKEDYGYVTRHKKKIL
                     NTYNRLHEALERGK"
     gene            8640..10037
                     /locus_tag="VC122_001173"
     CDS             8640..10037
                     /locus_tag="VC122_001173"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014407552.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent succinate-semialdehyde
                     dehydrogenase"
                     /protein_id="HEP5641098.1"
                     /translation="MAYQTIYPYTNEVLHTFDNMTDQDLADVLERAHLLYKKWRKEDH
                     LEERKAQLHQVANILRRDRDKYAEIMTKDMGKLFTEAQGEVDLCADIADYYADKADEF
                     LMSTPLETDSGQAYYLKQSTGVILAVEPWNFPYYQIMRVFAPNFIVGNPMVLKHASIC
                     PRSAQSFEELVLEAGAEAGSITNLFISYDQVSQVIADKRVVGVCLTGSERGGASIAEE
                     AGKNLKKTTLELGGDDAFIILDDADWDQLEKVLYFSRLYNAGQVCTSSKRFIVLDKDY
                     DRFKELLTKVFKTAKWGDPMDPETTLAPLSSAQAKADVLDQIKLALDHGAELVYGGEA
                     IDHPGHFVMPTIIAGLTKDNPIYYQEIFGPVGEIYKVSSEEEAIEVANDSNYGLGGTI
                     FSSNQEHAKAVAAKIETGMSFINSGWTSLPELPFGGVKHSGYGRELSELGFTSFVNEH
                     LIYIPNKTNNSNTKV"
     gene            10139..11935
                     /gene="uvrC"
                     /locus_tag="VC122_001174"
     CDS             10139..11935
                     /gene="uvrC"
                     /locus_tag="VC122_001174"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011054460.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="excinuclease ABC subunit UvrC"
                     /protein_id="HEP5641099.1"
                     /translation="MNELIKHKLELLPDSPGCYLHKDKEGTIIYVGKAKNLKKRVRSY
                     FRGSHDTKTELLVSEIVDFEYIVTESDTEALLLEINLIQKNMPKYNIKLKDDKSYPFL
                     KITNESFPRLVITRYIKKNDGLYFGPYPDSYTANEVKKLLDRIFPFKKCKNPINKVCF
                     YYHLGQCCAHTICHTDKAYWDRLIDDVKHFLNGKDDKIIEDLRSKMLAASEEMAFERA
                     AEYRDLISGIATMRTKQRVMSKDLQDRDIFGYYVDKGWMCVQVFFVRQGKLIQRDVNL
                     FPYYNDAEEDFLTYMGQFYQDKQHFIPKEVFIPEAIDEELVAAIVPTKIIKPKRGEKK
                     QLVALATKNARVSLQQKFDLLEKDIKKTSGAIENLGQLLKIDKPVRIEAFDNSNIQGT
                     SPVAAMVVFVDGKPSKKDYRKFKIKTVVGPDDYASMREVLFRRYSRVKKEGLQAPNLI
                     IVDGGVGQVNVAKDVIEKQLGLTIPVAGLQKNDKHQTHDLLFGNPLEVVPLPRRSEEF
                     FLLHRIQDEVHRFAVTFHRQVRRKNSFSSTLDHISGLGPKRKQLLLRHFKTITAIASA
                     TSEEIQALGIPKTVVEAIQQQITDNKNDRSSP"
     gene            12120..12722
                     /locus_tag="VC122_001175"
     CDS             12120..12722
                     /locus_tag="VC122_001175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011184478.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitroreductase family protein"
                     /protein_id="HEP5641100.1"
                     /translation="MKFLELNKKRHAIKTFNDQPVDYEDLRTAIEIATLAPSANNIQP
                     WKFVVVQEKKAELAKGLPLANKVQVEQAQYVVALFSDTDLALRSRKIARIGVKSLPDD
                     LIGYYMETLPPRFAAFNEVQTGEYLAINAGIVAMNLVLSLTDQKIASNIILGFDKSTT
                     NEILDIDPRFRPELLITVGYSDEKPEPSYRLPVDEVIERR"
     gene            12847..14256
                     /gene="pepV"
                     /locus_tag="VC122_001176"
     CDS             12847..14256
                     /gene="pepV"
                     /locus_tag="VC122_001176"
                     /EC_number="3.4.13.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002995681.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dipeptidase PepV"
                     /protein_id="HEP5641101.1"
                     /translation="MTTIDFKAEVDKRKEAMLADLVDLLRINSERDDRSADDQHPFGP
                     GPVKALEHFLAMAERDGYKTRNIDNYAGDFEFGQGDEVLGIFGHLDVVPAGSGWDTDP
                     YEPVIKDDRIYARGSSDDKGPTMACYYALKIIKELGLPVSKKVRFIVGTDEESGWGDM
                     DYYFAHNGLKNPDFGFSPDAEFPIINGEKGNITEYLHFAGDNKGAFVLHRFQGGLREN
                     MVPESATAVITAPHDLDVLEAALEQFLSEYGVKGSMKATDGKIEVTIIGKSAHGSTPE
                     AGINGATLLAKFLNQFTFEGAAKDYLHVAGEVLHEDFAAEKLGLAYTDDRMGALSMNA
                     GVFNFDSQSADNTIALNFRYPKGTDAATLKSGLEKLPGLTKVSLSEHEHTPHYVPMDD
                     ELVATLLAVYEKQTGLKGYEQVIGGGTFGRLLERGVAFGAMFPGDENTMHQANEYMPL
                     ENIYRSAAIYAEAIYELIK"
     gene            complement(14324..15700)
                     /gene="mnmE"
                     /locus_tag="VC122_001177"
     CDS             complement(14324..15700)
                     /gene="mnmE"
                     /locus_tag="VC122_001177"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002888396.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA uridine-5-carboxymethylaminomethyl(34)
                     synthesis GTPase MnmE"
                     /protein_id="HEP5641102.1"
                     /translation="MSITKEFDTITAISTPLGEGAIGIVRLSGTDALAIAQSVFKGKN
                     LEQVASHTINYGHIIDPKTGTIIDEVMVSVMLAPKTFTRENVVEINTHGGIAVTNEIL
                     QLLIRQGARMAEPGEFTKRAFLNGRVDLTQAEAVMDIIRAKTDKAMTIAVKQLDGSLS
                     QLINDTRQEILNTLAQVEVNIDYPEYDDVEEMTTALLREKTQEFQSLLENLLRTAKRG
                     KILREGLSTAIIGRPNVGKSSLLNNLLREDKAIVTDIAGTTRDVIEEYVNIKGVPLKL
                     VDTAGIRETDDLVEQIGVERSKKALQEADLVLLVLNASEKLTDQDRALLNLSQGSNRI
                     ILLNKTDLEQKIELEQLPADLISISVLTNQNINLIEDRINQLFFDNAGLVEQDATYLS
                     NARHISLIEKAVQSLEAVNDGLALGMPVDLLQVDLTRTWEILGEITGDAAPDELITQL
                     FSQFCLGK"
BASE COUNT         4830 a         2903 c         3263 g         4773 t
ORIGIN      
        1 cctcccaggt aaggacggta aggacggtca accaggtaaa ccggctccta aaacaccaga
       61 ggtccctcaa aaaccagata ccgcaccaca tacaccaaaa acccctcaga tccctggcca
      121 atcaaaagac gtgacacctg cccctcaaaa cccttctaat agaggtctaa acaaaccaca
      181 aacacaaggt ggtaatcagc tcgcaaaaac accagcagct cacgacacac acagacaatt
      241 gccagcaaca ggcgaaacaa ccaatccatt ctttacagca gctgctgtag ctatcatgac
      301 gacagctgga gttgtagctg ttgcaaaacg tcaagaaaac aactaatcct ctttttgcta
      361 cctaactgat ggaaagtaaa actcagagag aacctcaagg ttctctcttt tttgaatcaa
      421 gatagtagct ctaggtataa cgtcttgtct gaggtgctgt gaacaaatca gttaccactc
      481 acctcttttt ttggtaaaat gtaagcacta agacatcaaa aggagttttc catggaaaca
      541 tcagatgagt taaaacaacg cattggtgat ctttcttacg aggtcaccca gcatgcagca
      601 acagaatctc cctttacagg cgaatatgat aatttttttg agaagggtat ctatgtcgat
      661 attgttagtg gtgaggtctt attttcatca ctggataaat ttaattctgg ctgtggttgg
      721 cctgcctttt caaaacccat tgaaaatcgt atggtcacaa accacgatga ctcttcttat
      781 gggatgagac gagttgaggt taaaagccgt gaagcaggat cgcatttggg gcatgtcttt
      841 agtgacggcc ccaaagaagc tggaggcttg cgttactgta tcaattcagc agctcttaaa
      901 ttcattcctt atgagcagat ggaaaaagaa ggctatgccc agtggctcac attgtttgat
      961 gagacataac cgggtttcaa aatgactgaa aaagaagcgt cagtgattcc cacaagcact
     1021 ggattggtga ggcgtacggg tcaattttac aagcctttgt aaaaggtcta taatagggaa
     1081 tgaaaaaagg aggttagtat gtcaactcac ttaagaaaac ttccagggct gttactttgc
     1141 ttgttgttag ctcttccagc ctggtattta gggcgcttat ttcccattat tggagcacct
     1201 gtttttgcta ttcttttagg aatgttgtta gccttgtttt atcaccatcg tgacaaggct
     1261 aaagagggaa ttagttttac atccaagtat attttacaaa cagcagttgt tttgcttggt
     1321 tttggattaa acctaaccca agttatggca gtgggcatgc agtctttacc gattatcatt
     1381 tcaactattg cgacagctct tttggtagct tatggctcac agaaatggct gcgcttagat
     1441 gtcaatacag ccaccttggt gggtgtaggg tcttccattt gtggggggtc tgctattgca
     1501 gcgacggctc ctgtcattaa ggcaaaggat gatgaggttg ctaaggcaat ttcagtcatt
     1561 tttctcttta atatgttagc agctttgcta tttccaagtt taggacaatt actgggctta
     1621 tctaatgatg gttttgctac ttttgctggg acagctgtta acgacacctc ttccgtgact
     1681 gcaacggcca cggcctggga tgcccttcac cattccaata cactagatga cgcaaccatt
     1741 gtcaaattga ctcgcacctt ggctattctt ccaattactt taggtttatc cctttaccga
     1801 gcgaaaaaag agcacgacat cgttacagaa gaaaacttta gccttaggaa gtcattccct
     1861 cgtttcatcc tcatcttttt attagcttcc ctcatcacga cattgatgac cagctttggt
     1921 gtttctgccg atgttttcca ttccctaaaa accttatcaa aattctttat cgtgatggct
     1981 atggcggcta tcggtttaaa tacaaacctg gttaaattaa tcaagacggg cggtcaggct
     2041 atccttttag gagctatttg ctgggtagct atcacccttg tcagtttagc catgcaatta
     2101 agtttgggca tttggtaatc agacacgaga aatcagtttg actgatttct ttttttgtat
     2161 cttatttgtg tagaattgtc tacattttaa agagaatagt gcattttaaa aacgttttca
     2221 tttgataaaa taaacctatc gttaaaccag ttcagtaaag acaactggtt gagataagca
     2281 attaccgttt attcagcggt ttttgttaat agaggaggat aggaatgaaa cgaaaatttc
     2341 tcattgggag tcacggcaga ctagccagtg gtttgcaaag ctctattgac attttagcag
     2401 gtatgggaca ggcacttgaa accattgatg cttatgttga tgatagtgac tacactagcc
     2461 agattgataa ctttatcgct ggggtagcag cagatgagca gggccttatt tttacagatt
     2521 tgttaggcgg cagtgtgaat cagaaaatgg taaccgctgt aatgaacagt ggcaaagaca
     2581 atatcttttt aatcacaaac agtaacttag ccactttatt gtcgttggtg tttttaaaac
     2641 caggcgaagc gcttactaaa gatgatattg taaccgttat caatgaatcg caagttcagt
     2701 tagtggattt ggtccctgag acaaattcag aagatgactt ttttgattaa tatctaggag
     2761 gaagacaaat gattactcaa attcgtgttg acgaccgttt gattcacggg caagtggcag
     2821 ttgtatggac caaagagtta aatgcgccac ttttagtagt ggctaatgat gaagctgcaa
     2881 agaatgaaat cacacaaatg acgcttaaaa tggctgtgcc aaacggcatg aaactgctta
     2941 ttcgttcagt tgaagactcg attaagctct ttaatgatcc acgcgccaaa gacaagcgta
     3001 tttttgtgat tgtcaattct gttaaagatg cttgtgccat tgctaaagaa gttccagact
     3061 tagaagctgt taatgtggct aacgtggggc gttttgacaa atcagaccca gcaagcaaag
     3121 tcaaagtgac accaagtctt ttacttaacc cagaagaaat ggcagccgca aaggaattag
     3181 tcagtttacc agaacttgat gtctttaacc aagtcttacc atcaaatacc aaggtccatt
     3241 tgagccaatt agtcaactaa agagaggtaa aaaagtatgt tagtaccagc aacaatggca
     3301 gctttagctg tattgatttg ttttggggga aattatttaa ccggtcaaag catgatggaa
     3361 agacccctag tagttggact ggtcacaggt cttcttttag gagatatgaa ggtcggtatc
     3421 ttaatggggg cctctctaga agctcttttc ctaggaaatg tgaatattgg gggtgttatt
     3481 gcagctgagc ctgtaacagc gactgccatg gcaacaacct ttaccattat ttcacatatt
     3541 gaccaaaaag cagccatgac cctagctgtt ccaattggaa tgttggcagc ctttgtggtt
     3601 atgtttttga aaaacgtctt catgaatatc tttgctccaa tggttgataa agctgcagca
     3661 gctaaccatc aaggtaaact agtgatgctc cactatggca cttggattat ctattacttg
     3721 atcattgcct ctatttcttt cattggtatt ttagtaggta gtggacctgt taatgccttt
     3781 gtggaacaca ttcctcaaaa tctcatgaat ggcttgagtg ctgcgggagg ccttcttcca
     3841 gctgtcggtt ttgccatgct gatgaaatta ttgtggacca acaaattagc tgttttttac
     3901 ctattgggct ttgtcttaac agcttatttg aaattaccag cggttgcagt tgctgcccta
     3961 ggagcagtta tttgcgtcat tagttcccaa cgtgatctag aattagacgc aattactaga
     4021 ggtgcaatta gtaaacaaac aacctttgac tctaaagaat cagaagagga ggacttcttc
     4081 gcatgacatc acaagataat ttgactaaag aagaccgtaa aatgcttcgc tcagtctttt
     4141 ggcgctcatg gacgatgaat gctagtcgta ctggggcaac acaataccac gcagtagggg
     4201 ttatttacac tttactacca gtcatcaatc gtttttataa gactgataag gataaagcag
     4261 aggcactggt ccgtcataca acatggttca atgccaccat gcacatcaat aactttatca
     4321 tgggtcttgt ggcatcgatg gaaaagaaaa acagtgaaga tcctgatttt gatgctagtg
     4381 ctatcaccgc tgttaaggct tcgttgatgg gacctatatc tggagttgga gattccttct
     4441 tctggggaat tttaagggtt attgcggcag gtatcgggat ttccttggca agtgcaggct
     4501 cagccatggg agctgttgtc ttcttactct tgtacaatat tcctgctttt attattcact
     4561 actatagcct ttatggtggt tattctgtag gtgcaggatt tatcaaaaaa ctttacgaat
     4621 ctggtggtat aaaaatcgtc actaaaacct caagcatgct tgggttaatg atggtaggct
     4681 caatgacggc ttcaaatgta aaatttaaaa cgattcttac agttgctgcg aaaggtgcca
     4741 aagaagcagc ttctattcaa gattaccttg accaattgtt tattggtatt gtaccattaa
     4801 tggtaacact agcagctttt tggcttctac gaaagaaagt gaatatcaac tggattatgt
     4861 ttggtatcat gttccttggc attatccttg gacttttagg tatttgctaa taatctccta
     4921 gcaataaagg taagtaagac aagttttctt gttttgctta cctttttcct tttaagatga
     4981 tgagacttgt tttgatgaaa aacgtagtag cattaagaaa tttgttataa tgaaaggagc
     5041 ttaagatgcg aggtgaacag gtggaagaac attttaaaaa acaattacaa gatgatattt
     5101 ctagacattt ttcgtaccaa tcgttaatgt tatcattgtt gctaattggt ctttttatta
     5161 ttttttcatt agcgccacaa caattaggcc tctatcgtga tatcaatgcc actgcaaccc
     5221 gttatcaccg tttgattagc aaacaagaag ccttgcttga tgacttaggg aaaaatagct
     5281 tactaccttt tttaaataaa aacctcagca ctgctgattt aagcaagcac tattttcatt
     5341 tgcgccatag cagccaaacg tcaccagaac ttttactatt ttcacctagt caagaccttt
     5401 tatttgctag taacccacat ttaggaaatg tttttagtaa atctgtttat attcaagaag
     5461 tcttgagagc agctcatcct ccaaaaacct tgtttaaagt tgctatggat agtgaagatg
     5521 gtcactactt gatgatcatt aagccaatga ttgatcaaaa ccaactaaag ggatatgctt
     5581 ttttagtgat gagtggcaaa gattttcttc atcctacaaa aacactgacg tcagagttgg
     5641 tcattgctga taagctggac aatacgttta cgttttccaa tcgtgagttt atagcatcta
     5701 gtctagataa gatcaacagt cagtatttac accattattt tgtttttcaa gataatcgag
     5761 cctttatcac aagaaaggtt gccttacaag gaggtctttg gctttacatg tataggccct
     5821 tgattccaat ggtatcggtg atgttatttt cacttatttc atcagctgtt atctttgtga
     5881 tcttgcaacg taaatcaagt ggtttagcta accgaattgc agctaaaaat tcaagagcaa
     5941 tcaatcaaat ggttagagat atgagtgcga tctctcggca agaaaaaaga cgtattgacc
     6001 ttgagagtca agatgaattt caatatttat ctgatcaaat caatcaaatg gtagagcgat
     6061 tgcaacagtt acatgataaa acgttagatt tggaaactca aaaattatta tttgaaaaac
     6121 ggatgttaga agctcaattc aatccgcatt tcctctacaa tacgctagaa accattttga
     6181 ttacgagcca ttatgattct gccctaacag aaaaaattgt tatccaattg acaaaattgt
     6241 tgcggtatag tctcacggat tctagcaaac ctgtcttgct caaagatgat ttaagtgtca
     6301 tcgagtctta tttagtgatt aatcaggtgc ggtttgaaga attgcaatat agcattaacc
     6361 tatccccaga ccttgatagt cttgcagtgc ctaaattgtt cttgttacct ttgatagaaa
     6421 atgccatcaa atatggcttg aaagaacgac acgatgtcaa gattaacatt gcttgttatt
     6481 atcaagatga ccatattatt ttttctgtgc gagataatgg ttcgggaatt gatgctcatc
     6541 accaaaaagt gattcgagag caattggaag ctggagagtc acaccatggg ttaattaact
     6601 cttatcgtcg acttaagtat catttttcag aagtatcctt ggtttttgat caaggtgata
     6661 aacagtttaa tgttagctac catgttaagg agtagtctat gtacaaatta gtcattattg
     6721 aagatgaaca tattattagg aaatggttgc gatacgctat tgattataaa gctttagata
     6781 ttttggtcat tggcgaggct aaagatggca aagaaggagc tgttttaatc aaagagagtc
     6841 aaccagatat cgttttaact gatattaaca tgcccatcat gacagctttt gacatgtttg
     6901 aagcaacaaa agatcaaact tacgccaaaa ttattttatc tggctatgcc gattttccaa
     6961 atgcaagatc tgctatacac tacggtgtac ttgaattttt aacaaaaccg attgaaaaag
     7021 cagctctttg ggaatgtctt caaaccatta ttgctaaaat agaaaagcaa aaggggtcta
     7081 atcaaaaaac agatgcttgc gtctatattc ccttacccca gatgactgat cagattcctg
     7141 aagtagtcaa agatattttg gagtgggtac atgctcattt tcaagacaag atcagcacga
     7201 gccgcctagc tcatgactta gggtatagtg agagttatat ctatcaaaat atcaaaaagc
     7261 atcttcagat gcctttaagt gattacatta atcaatatcg gattaatcag gcaattcaat
     7321 tgatgcaaca agaaccagat ttaatggtat atgaaatagc acaagctgta ggtatttatg
     7381 actatcgcta ttttgatagg gtttttaaaa agtatctagg acaaactgta aaggctttta
     7441 aagaagagca ttttatgaag gataccgata ggagtaagcc atgttaaaac tcaaacgcaa
     7501 atggctatta agctttttgt tagtaataat tattctagca atcgttaaca ttgcaatgta
     7561 tatattctca tcgtcaaaaa aagatagtgc taaagaatta gttatcttga ctcctaatag
     7621 ccaaactatt ttaacaggga ctattccagc ctttgaggaa aagtatgggg ttaaagtaag
     7681 attaatccaa ggtgggacgg gccaacttat tgatcaatta ggtcgaaaag ataagccatt
     7741 aaacgctgat attttctttg gtggcaatta cactcaattt gaaagccata aagatttatt
     7801 tgaatcctat gtttctccgc aggtttctac tgtcatttca gattaccaat tgcctagtca
     7861 tcgtgcaacc ccatatacga tcaatggcag tgtactgatt gttaataacg aattagcaag
     7921 aggacttcat attaccagtt atgaggattt gctacaacca gctttaaaag gcaaaattgc
     7981 ttttgctgac ccaaatagct catctagcgc tttttcacaa ttaacaaaca tcttactggc
     8041 taaaggaggc tatacgagct cagaagcttg ggagtatgtg aaacgtttat tactgtctat
     8101 gaattctatc agagcaacaa gttcttcaga tgtttacgaa tctgttgcag aaggtaaaat
     8161 gattgttggt ttgacctatg aagacccttg catcaatctg caaaaaagcg gtgcaaacgt
     8221 ttcgattgtt tatccggatg aggggacagt atttgttccg tcatcagtcg ctattatcaa
     8281 aaaagcaaag gcaatgaaag aagctaagca atttattaat tttattttat catcagatgt
     8341 acaaaatgcc tttgggcagt caactagtaa tagacctatc cgacgagatg ctcaaacgag
     8401 tcatgaaatg aaaccattag atgaaattat tactttaaaa gaagattacg gatatgttac
     8461 tagacataag aaaaaaatat tgaataccta taatcgacta catgaagcat tagaaagagg
     8521 aaagtagact ctaggcttgt tgttgctata ctagtagtgt tacaaaatag cgtagtcatt
     8581 ttttgaaatc gatttcataa tctggtagac taagattatc acgaaaagga gataatataa
     8641 tggcttatca aactatttac ccttatacaa atgaagtcct tcatacattt gataatatga
     8701 ccgatcaaga tttggcagat gtccttgaaa gagctcacct tctctataaa aaatggcgca
     8761 aggaagatca tttagaagaa cgtaaagccc agcttcatca agtggctaat attcttcgcc
     8821 gtgaccgcga taaatatgct gaaatcatga ccaaagacat gggaaaactg ttcacagaag
     8881 cccaaggtga agtagatctt tgtgccgata ttgctgatta ctacgctgat aaggcagatg
     8941 aatttttgat gtctacgcca ctagaaacag attcaggtca agcttactat ttgaaacaat
     9001 caactggtgt tattctagct gttgaacctt ggaatttccc ttactatcaa atcatgcggg
     9061 tctttgcgcc aaactttatt gttggaaacc caatggtttt aaaacacgct tctatttgcc
     9121 cacgatctgc ccaatccttt gaagaattag ttcttgaagc tggtgctgaa gcaggtagta
     9181 ttaccaattt gtttatttct tatgaccaag tatcacaagt gattgctgat aaacgtgtgg
     9241 ttggtgtctg cttgacaggt tccgaacgtg gaggagctag cattgcagaa gaagctggta
     9301 aaaacttgaa aaaaacaacc cttgaattgg gtggcgatga tgcctttatt atccttgatg
     9361 acgcggattg ggatcaatta gagaaggtgc tttacttctc tcgtctctac aatgcaggtc
     9421 aagtatgtac ctcatcgaaa cgttttatcg tgcttgacaa agactatgac cgtttcaaag
     9481 agttattaac aaaagtcttc aaaacagcta aatggggtga tccaatggat cccgaaacga
     9541 cgttggcgcc attatcatca gctcaagcta aagcagatgt tcttgaccaa attaagttgg
     9601 ccttggatca tggtgcggag ttggtttatg gtggtgaagc gattgatcat ccaggtcatt
     9661 ttgtcatgcc aaccattatt gctggcctca caaaagacaa ccctatttac tatcaagaaa
     9721 tctttggccc agtcggtgaa atctacaaag tgtcttctga agaagaagcg attgaagtgg
     9781 cgaatgattc taactatggc cttggaggta ctatctttag tagcaatcaa gaacatgcga
     9841 aagccgtagc tgctaaaatt gaaactggta tgtcttttat caactcaggc tggacaagtc
     9901 ttccggaact cccatttggt ggggttaaac attcaggcta tggccgtgaa ttgagtgagc
     9961 ttggctttac atcttttgtg aatgaacacc ttatctatat tccgaataaa acgaataata
    10021 gcaatactaa agtttgatct agattgttaa agaagagtgt aacgccttaa taaacaatca
    10081 aggcgttaca ctcttctttt ttaaagtcaa aattttaaaa atttgataaa ctagtaacat
    10141 gaacgaactg attaaacata aactagaact cttaccagac agccctggct gttacttaca
    10201 taaagataaa gaaggaacta ttatctatgt cggtaaggct aagaatttga aaaaacgcgt
    10261 ccggtcctat tttcgtggga gccatgatac caaaacagag ttactagtat ctgagattgt
    10321 agatttcgaa tatattgtaa cagagtcaga taccgaagcg cttttgttgg aaattaactt
    10381 gattcaaaaa aatatgccta agtacaacat caagttaaaa gatgataaat cttatccctt
    10441 tctcaaaatc acaaatgagt cttttcctcg gttagtgatt acaagatata ttaagaaaaa
    10501 tgatggattg tactttgggc cctacccaga ttcttacact gctaatgaag ttaaaaagtt
    10561 attagatagg atttttccat ttaaaaagtg taaaaatcct atcaataagg tctgttttta
    10621 ttaccattta ggccagtgct gtgctcatac catttgtcat acagacaagg cctattggga
    10681 tcgtttaatt gatgatgtta agcacttttt aaatggtaaa gatgacaaaa ttattgaaga
    10741 tcttcgttct aagatgttag cagcttctga agaaatggca tttgaacgtg ccgctgaata
    10801 tcgagatttg atttcaggta ttgccaccat gcgcaccaag cagcgggtga tgagcaagga
    10861 tttgcaagat agagatatct ttggctatta tgttgataag ggatggatgt gtgtccaagt
    10921 tttctttgtg cgtcaaggga aattaattca gcgtgatgtt aacctttttc cctattataa
    10981 cgatgcagaa gaagatttct taacttatat ggggcaattt taccaagata aacagcattt
    11041 catccctaaa gaagtcttta ttccagaagc tattgatgaa gaactggtag ccgctattgt
    11101 gccaaccaaa atcatcaaac ccaaacgcgg tgaaaaaaaa caactggtag ctttggctac
    11161 taagaatgct cgtgttagtc tgcaacaaaa atttgattta ttagaaaaag atatcaaaaa
    11221 aacgagtggt gctatcgaaa atttaggtca gttacttaag attgacaagc ctgtgcgcat
    11281 tgaagccttt gataattcta acattcaagg gacaagtcca gttgcggcta tggttgtttt
    11341 tgttgatggc aaacctagta agaaagatta tcgtaaattt aagattaaaa cagtggtggg
    11401 accagatgat tatgccagta tgagagaagt gctttttcga aggtacagtc gggtgaaaaa
    11461 ggagggactg caagctccta acttgattat tgttgatgga ggcgttggac aagtcaatgt
    11521 ggccaaagat gtgatagaaa aacaacttgg actcactatt cctgttgcgg gacttcaaaa
    11581 aaacgataag caccagaccc atgatttgct ttttggaaac ccacttgaag ttgtaccgtt
    11641 gccgcgccgt tctgaagagt ttttcttact gcatcggatt caagacgaag tgcatcgctt
    11701 tgcagttaca tttcatagac aagtgcgacg taaaaattct ttttcatcaa cattagatca
    11761 tatttcaggg cttggcccaa agcgaaaaca gttattacta agacacttta aaaccataac
    11821 agcgattgca tcagctacct ctgaggagat tcaagcttta ggtataccta aaacagtagt
    11881 tgaagcaata cagcaacaga taacagacaa taaaaatgat agatcgtctc cttaaataag
    11941 tgttatgttt tcagtactga tgaggtaaaa aggcgctact tgttacgcac gacatttcag
    12001 ttcgttattt tgaaactttc agaaagttgc gaagtgtagt gagtttatga ttttaaaaac
    12061 tattttatgg taaaataaaa tggtattaaa aaaaacagat ttagatagga agttttgaaa
    12121 tgaaatttct agagttaaac aaaaaacgcc atgccattaa gacctttaat gaccagccag
    12181 ttgactacga ggatttgcga acagccattg agattgcaac cttagcacct agtgccaata
    12241 acattcaacc ttggaaattt gtagtcgttc aagagaaaaa agctgagtta gcaaaagggt
    12301 tacctctagc aaataaagtg caggttgaac aggctcaata tgtagtggcg ctattttcag
    12361 acactgattt agctttacgt tctcgtaaaa ttgcccgtat cggtgtcaaa tcgttaccag
    12421 acgacttaat tggttattat atggagactt tgccaccacg ttttgctgcc tttaatgagg
    12481 ttcaaaccgg tgagtactta gctattaatg caggcattgt ggctatgaac ttggtgttat
    12541 ccttgacgga tcaaaagatt gcttccaata ttatccttgg ttttgataag tcaacgacca
    12601 atgaaatttt agacatcgat ccgcgttttc gtccagaatt attgattact gttggctata
    12661 gtgacgagaa accagaacca agttatcgtt tgcctgttga tgaagtcatc gaaagaagat
    12721 agagtcagga caattagtta aatgtaatgt ccagatgcgc tacggagcga ccgctattgt
    12781 tagactgata ctattttggc ttattttaag ttaaacatta tccctaaaac gaataaggag
    12841 agtataatga caaccattga ttttaaagca gaagttgaca aacgtaaaga agctatgcta
    12901 gcagatttag ttgacctatt acgcattaat tcagaacgcg atgacagatc ggccgatgat
    12961 cagcatccct ttgggccagg tccagttaag gctttggagc atttcctcgc aatggctgag
    13021 cgtgatggct acaaaacgcg taatattgat aactatgcag gtgactttga atttggtcaa
    13081 ggtgatgaag tactaggaat ctttggccac cttgatgttg tgccagctgg tagcggttgg
    13141 gatacagacc cttatgagcc agtgattaaa gacgatcgca tctatgcgcg tgggtcttcg
    13201 gatgataagg gaccaaccat ggcgtgctac tacgctctga aaatcattaa agaattgggt
    13261 cttcctgtgt ctaaaaaagt tcgtttcatt gttggtaccg atgaagaatc tggctggggg
    13321 gatatggatt attactttgc tcataatggc ttgaaaaatc cagattttgg tttctcacca
    13381 gatgctgaat ttccaattat caatggtgag aaaggaaata tcaccgaata cctccatttc
    13441 gctggtgaca acaagggcgc ttttgtgctt catcgcttcc aaggtgggtt gcgtgaaaac
    13501 atggtaccag aatctgccac agctgtcatt acggcaccgc atgaccttga tgtgctagaa
    13561 gcagccttgg agcaattttt atcagaatat ggtgttaaag gctctatgaa ggctactgat
    13621 ggtaaaattg aagtaactat tattgggaag tctgctcacg ggtcaactcc agaagctgga
    13681 atcaatggtg ccaccttgct ggctaaattc ctgaatcaat ttacctttga aggggctgct
    13741 aaagattatt tacatgtggc aggtgaagtg cttcatgaag actttgcggc agaaaaacta
    13801 ggccttgctt ataccgacga ccgtatggga gccttgagca tgaatgcagg tgtctttaac
    13861 tttgatagtc aatcagctga caataccatc gccttgaatt tccgttatcc aaaagggact
    13921 gatgcggcaa ccttaaaatc aggattggaa aaattaccag gtttaactaa ggtaagcctt
    13981 tctgagcatg aacatacacc tcattatgtg ccaatggatg atgaattggt agcaaccttg
    14041 cttgctgttt acgaaaaaca aacaggtctt aaaggctacg aacaagttat cggcggagga
    14101 acctttggtc gtttgctaga acgtggtgtg gcctttggag caatgttccc aggtgatgaa
    14161 aacactatgc accaagctaa tgaatatatg cctttagaaa atatttatcg ctcagcagct
    14221 atttatgctg aagcaatcta cgaattaatt aaataagatg tttatgacag caaaaagagc
    14281 tggaacagtg ttccagctct tttgtgatga ttagagactg ttgttattta ccaagacaga
    14341 attgactaaa gagttgggta atgagctcat caggagcagc atcccctgtg atttctccga
    14401 gaatttccca agttctggtt aaatcaacct gtagtaaatc aactggcatg ccaagcgcca
    14461 agccgtcatt gacagcttct aaactctgga ctgccttttc aatcaaagaa atgtggcggg
    14521 catttgacag gtaagtggca tcttgttcta ctagtccagc attatcaaag aagagctggt
    14581 tgatacgatc ttcgatcaga ttaatgttct ggttggttaa caccgaaata gaaataaggt
    14641 ccgctggcaa ctgttccaat tcaatctttt gttccaaatc tgttttattc agcaatataa
    14701 tgcggttact gccctgactg agatttaaca aagcccggtc ttggtcagtc agtttttcag
    14761 aagcattcag cacaagaaga acaaggtcag cttcttgaag agctttttta gaccgttcaa
    14821 ctccgatttg ttcgaccaga tcatctgtct cacgaatacc ggccgtatcg acaagtttta
    14881 gaggaactcc cttgatatta acgtattctt caatgacatc tcgggtggtt cctgcaatat
    14941 cagtcacaat agccttgtct tctcgcaaca aattattaag taagcttgac ttaccgacgt
    15001 ttggacgacc aataatagct gttgataagc cctcacgcag aattttaccg cgtttagctg
    15061 ttcgaaggag attttccaaa aggctttgga attcttgtgt tttttcacgt agcagagcag
    15121 tggtcatttc ttccacatcg tcgtattcag ggtagtcaat attaacttcg acttgagcta
    15181 aggtgttgag aatttcttgt cgggtgtcat tgataagctg ggaaagggag ccatccagtt
    15241 gcttgactgc aatcgtcata gccttgtctg ttttggcacg aataatgtcc atgacagcct
    15301 cagcttgagt caaatcaaca cgaccattta agaaggcacg tttggtgaat tcgccaggct
    15361 ctgccattct agcgccttgg cgaatcaata gttgcaagat ttcatttgtc acagcaatac
    15421 caccgtgcgt attgatttca accacatttt cacgggtaaa ggttttgggg gctagcatga
    15481 ctgacaccat aacttcatcg ataatagttc ctgtcttagg atcaatgata tggccatagt
    15541 taatggtgtg tgaggcgact tgctctaagt tctttccctt aaaaacagat tgtgcaatgg
    15601 caagcgcatc tgtcccggat agacgaacaa tgccaatagc tccttcacca agaggggttg
    15661 aaatggcggt aattgtatca aattctttag ttatactcat gccctctatt ttaaatcaag
    15721 tcctagtaga aagcaaggta ttattttgta tagaaaatag gactagcac
//
LOCUS       DATXIG010000035        15528 bp    DNA     linear   BCT 28-DEC-2023
DEFINITION  TPA_asm: Streptococcus pyogenes strain MGAS11113
            SAMN08555799-rid19682583.denovo.035, whole genome shotgun sequence.
ACCESSION   DATXIG010000035 DATXIG010000000
VERSION     DATXIG010000035.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN08555799
            Sequence Read Archive: SRR6740495
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pyogenes
  ORGANISM  Streptococcus pyogenes
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 15528)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 15528)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-OCT-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-OCT-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001918058.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001918058.2
            Genome Coverage       :: 368x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/12/2023 11:23:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 1,806
            CDSs (total)                      :: 1,769
            Genes (coding)                    :: 1,722
            CDSs (with protein)               :: 1,722
            Genes (RNA)                       :: 37
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1, 1 (5S, 23S)
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 47
            CDSs (without protein)            :: 47
            Pseudo Genes (ambiguous residues) :: 0 of 47
            Pseudo Genes (frameshifted)       :: 30 of 47
            Pseudo Genes (incomplete)         :: 20 of 47
            Pseudo Genes (internal stop)      :: 9 of 47
            Pseudo Genes (multiple problems)  :: 11 of 47
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15528
                     /organism="Streptococcus pyogenes"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN08555799-rid19682583.denovo.035"
                     /strain="MGAS11113"
                     /serotype="Emm28"
                     /isolation_source="normally sterile site"
                     /host="Homo sapiens"
                     /db_xref="taxon:1314"
                     /country="Finland"
                     /collection_date="2002"
                     /collected_by="National Public Health Laboratory"
     gene            complement(22..2013)
                     /gene="uvrB"
                     /locus_tag="VC122_001178"
     CDS             complement(22..2013)
                     /gene="uvrB"
                     /locus_tag="VC122_001178"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002984194.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="excinuclease ABC subunit B"
                     /protein_id="HEP5641103.1"
                     /translation="MIDKRDDKPFKLKSKYKPSGDQPQAIESLVDNIEGGEKAQILLG
                     ATGTGKTYTMSQVISKVNKPTLVIAHNKTLAGQLYGEFKEFFPDNAVEYFVSYYDYYQ
                     PEAYVPSSDTYIEKDSSVNDEIDKLRHSATSSLLERNDVIVVASVSCIYGLGSPKEYA
                     DSAVSLRPGQEISRDTLLNQLVDIQFERNDIDFQRGCFRVRGDVVEVFPASRDEHAFR
                     VEFFGDEIDRICEIESLTGKTIGEVDHLVLFPATHFVTNDEHMEQSIAKIQAELAEQL
                     QLFESEGKLLEAQRLRQRTEYDIEMLREMGYTSGVENYSRHMDGRSPGEPPYTLLDFF
                     PEDFLIMIDESHMTMGQIKGMYNGDQARKQMLVDYGFRLPSALDNRPLRREEFESHVH
                     QIIYVSATPGEYEMSQTNTIIEQIIRPTGLLDPEIDVRPSMGQMDDLLGEINQRVARD
                     ERTFITTLTKKMAEDLTDYLKEMGVKVKYMHSDIKTLERTEIIRDLRLGVFDVLIGIN
                     LLREGIDVPEVSLVAILDADKEGFLRNERGLIQTIGRAARNVDGHVIMYADKMTDSMQ
                     RAIDETARRREIQIAYNKAHGIVPQTIKKDIRGLISISKTSHNDISKEEMDYESMSRG
                     ERKEAINALQKQMQEAAELLDFELAAQMRDLILELKLMD"
     gene            2205..4379
                     /locus_tag="VC122_001179"
     CDS             2205..4379
                     /locus_tag="VC122_001179"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002984191.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding
                     protein/permease"
                     /protein_id="HEP5641104.1"
                     /translation="MTHKIKVLLLAIMSIFLTCNIASAETIAIVSDTAYAPFEFKDSD
                     QIYKGIDVDIINEVAKRQSWDFSMSFPGFDAAVNAVQSGQASALMAGTTITNARKKVF
                     HFSEPYYDTKIVIATRKANAIKKYSDLKGKTVGVKNGTAAQAFLNNYKKKYDYTVKTF
                     DTGDLMYNSLSAGSIAAVMDDEAVIQYAISQNQDIAINMKGEPIGSFGFAVKKGSGYD
                     YLVNDFNTALKAMKADGTYQAIMTKWLGTDDKATTSQATGNPSAKATPIKDSYKIVSD
                     SSFAPFEFQNGKGKYVGIDIELIKAIAKQQGFKIEIANPGFDAALNAVQSSQADGVIA
                     GATITDARKAIFDFSDPYYTSNIILAVKAGKNIKNYEDLDRKTVGAKNGTSSYSWLKE
                     NAPKYGYNVKAFDDGSSMYDSLNSGSVDAIMDDEAVLKYAISQGRRFETPLEGISTGE
                     VGFAVKKGTNPELIEMFNNGLAALKKSGQYDDIIDKYLDSKKAATPSEKGADESTISG
                     LLSNNYKQLLAGLGTTLSLTLISFAIAIIIGIIFGMMAVSPTKSLRLISTVFVDIVRG
                     IPLMIVAAFIFWGVPNLIESMTGHQSPINDFLAATIALSLNGGAYIAEIVRGGIEAVP
                     AGQMEASRSLGLSYGTTMRKVILPQAVKLMLPNFINQFVISLKDTTIVSAIGLVELFQ
                     TGKIIIARNYQSFRMYAILAIIYLIMITLLTRLAKRLEKRLN"
     gene            4379..5119
                     /locus_tag="VC122_001180"
     CDS             4379..5119
                     /locus_tag="VC122_001180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003107174.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter ATP-binding protein"
                     /protein_id="HEP5641105.1"
                     /translation="MTELKIDVQDLHKSYGQNEVLKGIDAKFYEGDVVCIIGPSGSGK
                     STFLRTLNLLETITSGKVVVDGFELSDPKTNIDKARENIGMVFQHFNLFPHMTVLENI
                     IFAPVELGKESKEAAKKHGMALLEKVGLSDKADAFPGSLSGGQKQRVAIARSLAMNPD
                     IMLFDEPTSALDPEMVGDVLNVMKDLAEQGMTMLIVTHEMGFARQVANRVIFTDGGQF
                     LEDGTPEEIFDHPKHPRLIEFLDKVLNV"
     gene            complement(5267..5419)
                     /locus_tag="VC122_001181"
     CDS             complement(5267..5419)
                     /locus_tag="VC122_001181"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002989558.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEP5641106.1"
                     /translation="MTEKNGATFLMAVGFFLIAISPMVGNKYLHIVTGLALTGLGYYL
                     TKHKRP"
     gene            complement(5416..6795)
                     /locus_tag="VC122_001182"
     CDS             complement(5416..6795)
                     /locus_tag="VC122_001182"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012560769.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIC"
                     /protein_id="HEP5641107.1"
                     /translation="MSHFSDKFMEISGKIGSQRHLVAIRDSFISMMPITMAGSVAVLL
                     NVFLRDIPNNMGWTGFAKAMQPVIDINGYVYFGTIGIMALFFAFAFGYNLAVMHKINP
                     LAGGLISFGSFIATLPQTLTISTPLENASANLISNLKEMGLSVVTAGGASSIETSQWG
                     AIALKYVGATGLFTALIIGFLSSFVYAALTKRNITITLPDNVPPAVNKAFAAIIPGTV
                     AIYASAIFAYLIFALTGSSLSDVISTYIQLPLLGLSQGIGSVILLTFLVQLLWFFGLH
                     GHNVLAPVMDGIYMVALTENTAAYNTAHSAANLPYLWTRGSFDAYAQMGGSGVTLALI
                     IAIFIFSKREEHKTIAKLSAPMGIFNINEPITFGMPIVLNPTFVIPWLIVPPICASIA
                     YFATAIGLIPPVFLSVPWITPVGLYAYLATGGNIMAGLVSLFNLFVAFLIWAPFVILA
                     NKEKASDLA"
     gene            complement(6973..7422)
                     /locus_tag="VC122_001183"
     CDS             complement(6973..7422)
                     /locus_tag="VC122_001183"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015017132.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3284 domain-containing protein"
                     /protein_id="HEP5641108.1"
                     /translation="MIISAEGDVSVHHCFEAMLISLQEDYRQNTLRQLSRDDIKAGIN
                     YVKHYGKYKERTVRVLITALEAPQLYTACFSSNHGIKELAYRLEALGENRTRITYSYD
                     YHPLDIFQKANYFIVGKLFKKSLERQSQAQLAALIQYAKQLQQPSSL"
     gene            complement(7419..7754)
                     /locus_tag="VC122_001184"
     CDS             complement(7419..7754)
                     /locus_tag="VC122_001184"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002984173.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS lactose/cellobiose transporter subunit IIA"
                     /protein_id="HEP5641109.1"
                     /translation="MTEPSNLEAIMGLIMYGGEAKGKAVEAIHAARDGQFEEAQHLLQ
                     EATNSLNIAHKSQTHLLSQEAAGDAVELSLLMVHGQDHMMTALAFIDLAKELVTVYDK
                     MSAIQRGES"
     gene            complement(7757..8068)
                     /locus_tag="VC122_001185"
     CDS             complement(7757..8068)
                     /locus_tag="VC122_001185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010922394.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HEP5641110.1"
                     /translation="MKSIMLVCNAGMSTSMLVTKMQKAAQARDLEVSIWAVPVSEADN
                     EVAANSIDVLLLGPQVKFLLKDFKDKFEPDIKVDAINMADYGLMNGEKVLETALAMME
                     E"
     gene            complement(8091..10085)
                     /locus_tag="VC122_001186"
     CDS             complement(8091..10085)
                     /locus_tag="VC122_001186"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003061111.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BglG family transcription antiterminator"
                     /protein_id="HEP5641111.1"
                     /translation="MLSKKELAIITFLIHHKEQFVSSATLAEVIGMSDRTVRKYLKEL
                     ISSLPEHGAHIISKQGRGYCLEIDHSMAFDIFWQESVTSKKRLADVTQVEESVDREDY
                     VLHKFFFEDAVQDFEELCQELYISRTTLKHVLAGIKERIIPYQLELEITHQHIQIRGK
                     EEDIRHFIMDYFFVTSFDNTLSTMVGNTFLEGINFAEIMIIVLDECRDAKLKLSDFVM
                     NNLVLHIALMVQRIRSGCPLELFSIPIAIRQSDEYQVALRILYRVEEVMGIRFPKEEA
                     NYIALHLKVKHSVGKHWQDDNTDEKLQDHLKACIAKISQLTGMTLETDTNLFQGLLAH
                     MMPLTTRLENHIQLTNPLTEEIKSQYPEIFTLTKQTFSDLLVCQKNDVSDDEWAYISL
                     HLMAAIERYSNRHKLRVLVVCATGYGSAMMLKNRLEKEFEGRLRIVDVISYYEITEER
                     LKTVDLIISSISLANLMFLTPVITVSVFLSNQDIETIRQFIGEQEGIKKEVSLPSQMS
                     LAKAEQLLTGVFSPNRFLYLDEKISKEDLLLRMIACLDEAGTETFVEDFYHQMVLREN
                     YSPVIYGEVLAFPHPANPMTYSEQVVVAICREPLEWDKAHQAVHFVFLLSPSKGHNHR
                     LKYVSPGLASFVNQVELQQALLEEPNYSKFMTVFTPLIHD"
     gene            complement(10191..11285)
                     /locus_tag="VC122_001187"
     CDS             complement(10191..11285)
                     /locus_tag="VC122_001187"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011888821.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF871 domain-containing protein"
                     /protein_id="HEP5641112.1"
                     /translation="MVDLGFSLYPERYDVTKSKAYIDLCHSYGAKRLFMSLLQLAPAD
                     HQMFHCYAELIAYANQLGIRVIADVSPSFISQAGWSDQLIERAHAFGLAGLRLDEALP
                     LAEIVTLTRNPFGLKIELNMSTDKQLLMSLLATDAEQSNIIGCHNFYPHEFTGLSWQH
                     FKDMSRFYHEHDIETAAFITAQSASEGPWLLAEGLPTVEDHRHLPIGLQVELMKAIGT
                     IDNILISNQFISEEELAACTQALARPVTTIKVRPIIDLTEVEEKIIGYPHCYRGDVSD
                     YVIRSTMPRLVYAQESIAPRDQSKEVKRGSIIIDNDRYHRYKGELQIALKNFTVSSKA
                     NVVAEVREDYLSLLDDLRPWQEFCLEIDPS"
     gene            complement(11294..12694)
                     /locus_tag="VC122_001188"
     CDS             complement(11294..12694)
                     /locus_tag="VC122_001188"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014612380.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 1 protein"
                     /protein_id="HEP5641113.1"
                     /translation="MKQRKHRYQFPDGFLWGSSTSGPQSEGTVPGDGKGPSNWDYWFS
                     IESAKFHHQIGPEKTSTFYENYKGDIALLKETGHTIFRTSIQWSRLIPEGVGEVNPKA
                     VTFYREVFQDIIAQGIKLIVNLYHFDLPYALQEKGGWENKATVWAYETYAKTCFELFG
                     DLVNTWITFNEPIVPVECGYLGYYHYPCKVDAKAAVQVAYNTQLASSLAVKACHKLHP
                     DHKISIVLNMTPAYPRSDAPEDVKAARIAELFQTKSFLDPSVLGVYPAELVSILEEAD
                     LLPQYSADELEVIKNNTVDFLGVNYYQPLRVQAPSKSQQEGDPLILDIYFEPYDMPGK
                     KVNPHRGWEIYEPGLYDIALNLKEHYGNIEWLVTENGMGVEGEEAFLADGQIQDDYRI
                     TFIEDHLIQLHKALGEGANCKGYLLWTFIDCWSWLNAYKNRYGLVALDLESQKRTIKK
                     SGYWFKALSESNGFDK"
     gene            12919..13614
                     /locus_tag="VC122_001189"
     CDS             12919..13614
                     /locus_tag="VC122_001189"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011017909.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nicotinamide riboside transporter PnuC"
                     /protein_id="HEP5641114.1"
                     /translation="MAYLNYFTKTEWALWLSSVLAILISALLFGKQAPLALIASLIGV
                     TSLIFSAKANPIGQGLVIIFSIIYAYLSLRNSYYGELMTYLFMTLPMTIFSLFTWLNH
                     PFEGKKSQVTISRLTPTDRRCLFVFTILVTLIFYGILASIQTAYLLVSTLSIATSFSA
                     VYLSYKRSPYFALAYGLNDLVLILLWMHAAQTDASQYAVVICFATFLINDSYTFYSWL
                     MLQRYQEKKVKKG"
     gene            complement(13619..13777)
                     /locus_tag="VC122_001190"
     CDS             complement(13619..13777)
                     /locus_tag="VC122_001190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012515888.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEP5641115.1"
                     /translation="MGDKPISFKDKDGNFVSAADVWNAEKLEELFNLLNPNRRLRLER
                     EKLKKDEA"
     gene            complement(13846..15159)
                     /gene="obgE"
                     /locus_tag="VC122_001191"
     CDS             complement(13846..15159)
                     /gene="obgE"
                     /locus_tag="VC122_001191"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002984148.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GTPase ObgE"
                     /protein_id="HEP5641116.1"
                     /translation="MSMFLDTAKISVQAGRGGDGMVAFRREKYVPNGGPWGGDGGKGG
                     SVIFRVNEGLRTLMDFRYNRKFKAKSGEKGMTKGMHGRGAEDLIVFVPQGTTVRDAET
                     GKVITDLVEHGQEVVIAKGGRGGRGNIRFATPRNPAPEIAENGEPGEERQLELELKIL
                     ADVGLVGFPSVGKSTLLSVVSSAKPKIGAYHFTTIVPNLGMVRTKSGDSFAMADLPGL
                     IEGASQGVGLGTQFLRHIERTRVILHVIDMSASEGRDPYEDYVSINNELETYNLRLME
                     RPQIIVANKMDMPEAQENLKAFKKKLAAQYDEFDDLPMIFPISSLAHQGLENLLEATA
                     ELLAKTDEFLLYDESDLVDEEAYYGFAEAEKEFEITRDDDATWVLSGEKLERLFVMTN
                     MERDESIMKFARQLRGMGVDEALRERGAKDGDLVRIGKFEFEFVD"
     gene            complement(15216..15344)
                     /locus_tag="VC122_001192"
     CDS             complement(15216..15344)
                     /locus_tag="VC122_001192"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002261953.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4044 domain-containing protein"
                     /protein_id="HEP5641117.1"
                     /translation="MAFGENGPRKKTTFEKVTMVVVILMVLVTVGGLIASALSVLM"
BASE COUNT         4667 a         3326 c         2791 g         4744 t
ORIGIN      
        1 aatataagct ttattcaaaa cctagtccat taatttaagt tctaaaatca agtctcgcat
       61 ttgagctgcc aattcgaaat caagtaattc agctgcctct tgcatttgtt tttgcagagc
      121 attgatcgct tcttttcgtt ctcctcttga catactttca taatccatct cttctttcga
      181 tatatcattg tggcttgttt tacttatact aataaggcct ctgatgtctt tttttatagt
      241 ttgtggaacg atgccgtgcg ctttgttata tgctatttga atctcacgac gacgagccgt
      301 ttcgtcaata gcccgctgca tagaatctgt catcttgtcc gcatacataa tgacatgacc
      361 atctacgttt cgagctgctc taccgatggt ctgaatcaag ccgcgttcat tacggaggaa
      421 accctcttta tcagcatcca aaatggctac aagactaact tctggcacat caatcccttc
      481 acggagcaga ttgattccaa tcaagacatc aaaaacacca agtcgtaaat cacgaataat
      541 ttctgtccgc tctaaggttt taatatcact gtgcatatac ttgaccttga cacccatctc
      601 ttttagataa tcagtcaaat cttctgccat ttttttagtt aaggttgtga tgaacgtccg
      661 ttcatcgcgc gctacgcgct ggttaatttc tcccaacaaa tcatccattt gccccatact
      721 tggccttaca tcaatttcag gatcaagtag cccagtcgga cgaataattt gctcaataat
      781 tgtattagtc tgtgacattt cgtattcacc aggcgttgct gaaacataga taatttggtg
      841 cacatgactt tcaaattcct cgcgtcttaa gggacgatta tcaagagctg atggcaatcg
      901 aaaaccatag tcaactagca tttgttttct ggcttggtca ccattataca tgcctttgat
      961 ttgtcccatt gtcatgtgac tttcatcaat cataattaaa aaatcttctg ggaagaaatc
     1021 taacaaggta taaggaggtt ctcctggtga cctgccatcc atatgacggg aatagttttc
     1081 gactccgcta gtatagccca tttcacgtaa catttcaata tcatattccg ttctttggcg
     1141 taatcgctgt gcttctagta gtttaccttc agattcaaat aactgtaact gctctgctaa
     1201 ctctgcttga attttggcta ttgattgctc catatgctca tcatttgtta caaagtgagt
     1261 agctggaaat aaaaccaaat ggtcaacttc tccaatagtt ttcccagtta aactttcgat
     1321 ttcacatata cgatcaatct catccccgaa aaattccacc cgaaaagcat gttcatccct
     1381 ggatgctgga aagacctcaa caacatcgcc acgtacacga aaacagccgc gttgaaaatc
     1441 aatatcatta cgctcaaatt gaatatctac taattgattt agcaaagtgt cacgtgatat
     1501 ctcctgacct ggacgtaggc tgactgctga atctgcgtac tcttttggcg aacctagtcc
     1561 ataaatacaa gatacagaag ctaccactat gacatcgtta cgctcaagta aagaagaggt
     1621 ggctgaatgg cgaagtttat cgatttcatc attgactgaa ctatcttttt cgatataggt
     1681 gtcgcttgat gggacatatg cttcaggttg gtagtaatca taataggaaa caaagtattc
     1741 cacagcattg tctggaaaaa attctttaaa ttcaccatat aattgtcctg ctaaggtttt
     1801 attgtgagct atcactaagg taggtttatt tactttacta attacttggc tcatggtgta
     1861 tgtttttccc gttcctgtag cacctaggag aatctgagct ttttctccgc cctcaatatt
     1921 atcaactagg ctttcaatag cttgtggctg atcaccagac ggtttatact ttgattttag
     1981 tttaaatggt ttatcatcac gtttatcaat cattttatac cctctaatag tcttatctct
     2041 tatagactat tttaacatag ataagccaga atcacctcca ataagcgtaa aaaacatgtc
     2101 atttacatgt tatgttttat gacatataaa taacaaattt cttgcttaat gttcaacatt
     2161 ttgttataat taaccatatt ttttagtttt ataaggagat tagtatgacg cacaaaataa
     2221 aagtattgct gcttgcgata atgtctattt ttttgacatg caatattgca agtgctgaaa
     2281 ctattgctat tgtttcagat acagcttatg ccccatttga atttaaagac tcagatcaaa
     2341 tttacaaagg aattgacgtt gatattatta atgaagtagc caaacgtcaa tcttgggatt
     2401 tcagtatgag tttcccgggt tttgatgcag ctgtaaatgc tgtacaatct ggtcaagcga
     2461 gtgctctaat ggccggtaca accattacga atgctcgtaa gaaagtcttt catttctcag
     2521 agccatatta cgataccaaa attgtcattg cgacacgtaa agccaatgcc atcaaaaaat
     2581 acagtgactt aaaaggaaaa acggtcggtg ttaaaaatgg aacagcggct caagcctttt
     2641 tgaataacta taaaaaaaag tatgattata ctgttaaaac atttgacaca ggtgatctta
     2701 tgtataatag tttatctgct ggttctattg ccgctgttat ggatgatgaa gcggttatcc
     2761 aatacgcaat cagccaaaac caagatattg ctattaacat gaaaggagag cccattggaa
     2821 gctttgggtt tgctgtcaaa aagggaagcg gatatgatta tctagttaat gatttcaata
     2881 cagctcttaa agctatgaaa gctgatggta cctaccaagc tatcatgacc aagtggttag
     2941 gcacagatga taaagctacc accagtcagg caacgggaaa tccatctgcc aaagctacac
     3001 ctataaagga cagttataaa attgtctctg attcgtcttt tgcacctttt gaatttcaaa
     3061 atggtaaggg caaatacgtt ggtattgaca tagaattaat caaagctatt gctaaacaac
     3121 aaggtttcaa aattgaaatc gctaatccag gtttcgatgc tgccttaaat gctgtgcaat
     3181 ctagccaagc agatggggtc attgctggtg caactattac tgacgctcgt aaagctatct
     3241 ttgatttttc tgatccttat tatacttcta atatcatttt agctgttaaa gctggaaaaa
     3301 acatcaagaa ctatgaagac ttagacagaa aaacagtcgg tgctaaaaac ggcacttcat
     3361 cttactcttg gttaaaagaa aacgctccta aatatggtta taatgtcaag gcatttgatg
     3421 atggttctag catgtatgat agcttaaatt caggttctgt agatgctatc atggatgatg
     3481 aggcggttct taaatacgct atttctcaag gtcgtcgctt tgaaacacct cttgagggca
     3541 tttctactgg tgaagttggt tttgctgtca agaaaggaac taatccagaa ttaatcgaaa
     3601 tgttcaacaa tggcttagct gctctcaaaa aatctggtca gtatgatgac attatagata
     3661 aataccttga ctctaagaaa gctgcaactc cttctgaaaa aggtgctgat gagtctacta
     3721 tttcaggcct attatcaaat aactacaaac aactattggc aggacttgga accacgctca
     3781 gtttaaccct tatttcattt gctattgcta taattatcgg gatcatcttt gggatgatgg
     3841 ccgtgtcacc aactaaatca cttcgactca tttcaacggt ctttgtagac attgttcgag
     3901 ggattccttt gatgattgtg gctgccttca ttttctgggg agtaccaaac cttatcgaga
     3961 gtatgaccgg ccaccagtca ccgattaatg atttcttagc tgctacaatt gcactgtcac
     4021 ttaatggcgg agcctatatt gctgaaattg ttcgcggtgg tatcgaagct gttccagcag
     4081 ggcaaatgga agctagtcga agtcttggtt tgtcttacgg aaccacgatg agaaaagtaa
     4141 ttctcccaca agctgtgaaa ctaatgttac ctaactttat caatcagttt gttatttcat
     4201 tgaaggatac aacaatcgtc tcagcaattg gtttagtgga actcttccaa acaggtaaaa
     4261 tcattattgc tagaaattac cagtcgttcc gtatgtatgc tattttagca attatttacc
     4321 ttatcatgat tacactctta acaagacttg caaaacgttt agaaaagagg cttaactaat
     4381 gacagaatta aaaattgatg ttcaagattt acacaaatct tatggtcaaa atgaagtctt
     4441 aaaaggaatt gatgccaaat tctacgaagg tgatgtcgtt tgtatcattg gtccttctgg
     4501 ttctgggaaa tcaacctttc tacgtaccct caaccttcta gagacgatta ccagtggtaa
     4561 ggtcgtggtg gacggttttg aattatcaga tcctaagact aatattgaca aggcacgtga
     4621 aaatatcggg atggtattcc aacacttcaa ccttttccct cacatgactg ttcttgagaa
     4681 tattatcttt gctcctgttg aattaggtaa agagtctaaa gaagctgcta aaaagcacgg
     4741 catggcactt ctagaaaaag tcggactttc tgataaggcc gacgccttcc caggaagttt
     4801 atccggcggg caaaaacagc gtgtcgccat cgccagaagt ttagcaatga acccagatat
     4861 tatgctcttt gatgaaccaa cttctgctct tgaccccgaa atggttgggg acgttttgaa
     4921 cgttatgaaa gacttggcag aacaaggcat gaccatgttg attgttactc acgaaatggg
     4981 gtttgctcgt caagtagcta accgcgttat ctttacagat ggtggtcaat ttttggaaga
     5041 tggaactcct gaagaaattt ttgaccatcc taagcaccct cgtctcattg aatttttaga
     5101 caaggtctta aacgtctaat agagcagaca ccatactaac ttccaaaaaa taataaggca
     5161 aggaaacagg tttccttgcc ttttttggtt actgttatta ttcaaaccag caaaataggg
     5221 atactcttat gactctctac ttcataagag tgtcccctat gcttttttat gggcgtttat
     5281 gtttagtgag gtaataacct aatcccgtaa gagctagtcc cgtcactatg tgtaagtatt
     5341 tgttaccaac catcggacta atggcaatga gaaaaaagcc aacagccatt aaaaaagttg
     5401 ctccattttt ttctgtcatg caaggtcact cgctttttct ttattggcca aaatgacaaa
     5461 tggtgcccag ataaggaagg caacaaagag gttaaacagt gaaaccaaac ctgccataat
     5521 attacctcca gtagctaggt aagcataaag tccaacaggg gtaatccaag gtactgataa
     5581 gaataccggt ggaatcaaac ctattgcagt agcaaaataa gctatgctag cacaaattgg
     5641 tggcacaatt agccatggaa tcacaaaggt tggattcaaa acaattggca tcccaaatgt
     5701 aattggttcg ttgatattga aaatacccat tggtgcggac aatttggcaa tggttttatg
     5761 ctcttctcgt tttgagaata tgaagatagc aataatcaat gccaaggtta ctcctgaacc
     5821 acccatttga gcataagcat caaaggaccc gcgtgtccaa aggtatggta aattagcagc
     5881 gctatgcgct gtattgtaag cagctgtatt ttctgttaaa gctaccatat aaataccatc
     5941 catcactggg gctaagacat tatgaccatg caagccgaag aaccagagca gttgaactaa
     6001 gaaggttaac aagataactg aaccaatacc ctgtgataag cctagcaatg gtaattggat
     6061 ataggttgaa atgacatcac ttagggaaga acctgttaat gcgaagatga ggtaggcgaa
     6121 aattgccgaa gcgtaaatcg caactgttcc tggaataatg gcagcaaagg ctttattaac
     6181 cgcaggtggt acattatctg gtaaggtaat ggtaatatta cgtttggtca aagccgcata
     6241 cacaaaactt gataaaaagc caataattaa agctgtgaac aagcctgtgg caccgacata
     6301 ctttagagct attgctcccc attgactagt ttcaatactt gaagctccac ccgcagtaac
     6361 gactgaaagc cccatttctt tcaaattgct aattaggtta gcagaggcat tttcaagtgg
     6421 tgtcgaaatg gttaaagtct gaggtagggt ggcaataaat gagccaaaag aaatcaatcc
     6481 tcccgccaat ggattaatct tatgcataac agctaaattg taaccgaaag caaaggcaaa
     6541 aaagagcgcc atgataccaa ttgtcccaaa ataaacatag ccattgatgt cgataacagg
     6601 ttgcatagct tttgcaaacc ctgtccatcc catattgttt ggaatatcgc gtaaaaaaac
     6661 gttcaataag acagcaactg aacctgccat ggtaataggc atcattgaaa taaaggaatc
     6721 acgaatagct actaaatgcc gttgtgaccc aatttttcct gaaatctcca taaacttatc
     6781 agaaaaatgc gacataaatc ttctcctctt tttatttttt taatgtaaca aaggtaacct
     6841 taaaagcagc ggtgtctgcc taacacaatt atctcaaaat catccttgtt aaacgtgatg
     6901 cttagagata accggtaatc ggctcttatc caaatgaacc cttctctctc attcaaggtc
     6961 tatgaataat cttcacaaag acgatggttg ctgcaactgt ttagcatatt ggataagggc
     7021 tgctaactgg gcttgagatt ggcgttctag gctttttttg aacagtttgc caacaataaa
     7081 ataatttgct ttttgaaaaa tatctagagg atgatagtca taagaatagg taattctagt
     7141 ccgattctcc cccagagcct ctaatcgata agccaattcc tttatcccat gattggaact
     7201 aaaacaagca gtatataatt gcggagcttc tagggccgta ataagcaccc tcactgtacg
     7261 ctctttgtat ttaccataat gtttaacata gtttatcccc gctttgatat catctcttga
     7321 caactgtctc aaggtatttt gtcgataatc ttcttggagc gatatcagca tggcttcaaa
     7381 acaatgatgg acggaaacat ctccttctgc tgaaatgatc atgactcacc tctttgtatt
     7441 gctgacattt tgtcgtaaac ggtaaccaat tccttggcta aatcaataaa ggctaaggca
     7501 gtcatcatgt gatcttgccc atgcaccatc aataaagaca attcaaccgc atcaccagct
     7561 gcttcttggg acagcaaatg tgtctgcgat ttatgggcaa tatttaacga gtttgtcgct
     7621 tcttgaagta agtgctgagc ctcctcaaac tgcccatctc gtgctgcatg aatagcctct
     7681 acggccttgc ccttggcttc gcctccatac atgattaagc ccataatagc ttctaagttg
     7741 gatggttccg tcatgcctac tcctccatca tcgctagggc tgtttcaagg actttttcac
     7801 cgttcataag accataatca gccatgttaa tggcatctac tttaatatct ggttcaaact
     7861 tatctttaaa atcctttagt aagaatttga cttgtggacc taacaagaga acgtcaatgc
     7921 tattagcagc cacttcgttg tcagcttcac tcacaggaac tgcccaaata gaaacttcaa
     7981 ggtcacgcgc ttgggctgct ttttgcatct tagtcaccaa catactagtt gacataccgg
     8041 cattacaaac taacataata gatttcataa ggtttctcct tttaatcgtt ttaatcatga
     8101 ataagtgggg taaacactgt catgaatttg gaataatttg gttcttctag taacgcttgc
     8161 tgaagctcca cctgattgac aaaagatgcc aaacctggtg atacatattt taacctatga
     8221 ttatgccctt tagacgggga taatagaaag acaaaatgaa cagcttgatg cgccttgtcc
     8281 cactcaagag gctctcggca aatagccact actacctgtt cagaataagt catgggatta
     8341 gcaggatgcg gaaaggcaag cacttctcca tagatgactg ggctgtaatt ttccctcagt
     8401 accatttgat gataaaagtc ctctacaaac gtttctgttc ccgcctcgtc aagacaagca
     8461 atcatccgta aaagcaagtc ttcctttgag attttctcgt ctaaataaag aaaacgatta
     8521 gggctaaaaa cacctgttag aagctgctct gcttttgcta aactcatctg tgacggtaaa
     8581 ctgacctctt tcttgatacc ttcttgttct ccaataaatt gtcgaatagt ttcaatatcc
     8641 tgatttgaca aaaaaacact gacagtaatc actggtgtta aaaacatcag attggcaagg
     8701 ctgatagagg aaataatcaa atcaacggtt ttcagtcgct cttcggttat ttcatagtaa
     8761 ctgataacat cgacaatacg caaacgtcct tcaaattcct tttccaagcg atttttcaac
     8821 atcatggcac taccataacc tgtggcacaa actactagaa ccctcaattt atgacggttt
     8881 gaataacgct caatggccgc cattaaatgc aaactaatat aagcccattc atcatctgag
     8941 acatcattct tttgacatac aagcaaatcc gaaaaggtct gcttggtaag ggtaaatatt
     9001 tcaggatact ggcttttaat ttcctcagtt aaaggattag tcagctgaat atggttttca
     9061 agacgtgtgg tcaagggcat catgtgggct aataaacctt gaaataaatt ggtatctgtt
     9121 tctaaagtca tgcctgtcaa ttgactaatc ttggcaatac atgcttttaa atggtcctgc
     9181 aacttttcat cggtattatc atcttgccaa tgcttaccaa cagaatgttt gacctttaga
     9241 tgcagtgcaa tataattagc ctcctcttta ggaaaacgta tccccatcac ttcttccact
     9301 cgatacaaaa tgcgcagagc tacttgatac tcatcagact gacgaatagc tattggaatg
     9361 gaaaacagtt ctaatggaca ccccgatcga atacgctgaa ccattagggc aatatgcaag
     9421 actagattat tcatgacaaa atcagaaagt ttcaacttag catcccgaca ttcatccaag
     9481 acaataatca tgatttctgc aaaattaata ccttcaagaa aggtattacc taccatggtg
     9541 gacaaggtgt tatcaaaact agtaacaaaa aaataatcca tgataaaatg cctaatgtcc
     9601 tcttctttac ctcttatttg aatatgttgg tgagttattt caagctctaa ctggtaaggt
     9661 attatgcgct ccttaatgcc tgctaaaaca tgttttaaag ttgtccgact gatatacaat
     9721 tcttgacaaa gctcttcaaa atcctgaacg gcatcttcaa aaaagaattt atgaagcaca
     9781 taatcttctc gatcaaccga ttcttccact tgagtcacat ctgctaaacg ttttttagac
     9841 gtcacacttt cttgccaaaa gatgtcaaat gccatagagt ggtcaatttc taagcagtaa
     9901 cctcgacctt gtttagaaat gatgtgagct ccgtgttcag gaaggctact gatcagttct
     9961 ttgaggtatt tcctaaccgt acggtcactc ataccaataa cctcagccag agttgcacta
    10021 gagacaaact gctctttgtg atggattaga aatgttatga tggccagttc tttttttgat
    10081 aacattagtc ctccttacta ctttagaaaa cgctctctac cctcttttag tgtaccaaat
    10141 atttcctgaa aaaggtgacc atcattttcc gcattgattg tgaaaagaat ctatgatggg
    10201 tcaatctcca aacaaaattc ctgccaaggt cgcaaatcat ctaacaaact aagatagtcc
    10261 tctctgacct cagcaaccac attagctttt gaactaaccg taaaattttt caaagcaatt
    10321 tgcagttctc ccttatatcg atgatagcgg tcattgtcaa taataatgct acctcgtttg
    10381 acctccttag attggtctct tggagcaatc gattcctgag cataaactag acgtggcatg
    10441 gttgatcgaa tcacataatc agatacatcg ccacgataac aatgcggata gccaatgatt
    10501 ttctcctcaa cttctgtcaa atcgatgata ggcctaacct tgatcgttgt gactggtctt
    10561 gctaaagcct gagtacatgc tgccaattct tcttccgaaa tgaattggtt agatatgagg
    10621 atattatcaa tggttcctat cgctttcatt aattctactt gcaggccaat gggcaagtgc
    10681 ctatggtctt ccactgtcgg caagccttct gccagtagcc aagggccttc agatgctgac
    10741 tgagcggtaa taaaagcagc cgtttcaata tcatgctcat gataaaaacg tgacatatcc
    10801 ttaaaatgtt gccatgacaa accggtaaat tcatgtggat aaaaattatg gcaaccaata
    10861 atattgctct gttctgcatc tgtcgctagc aatgacatca ataattgttt atcagtgctc
    10921 atattaagtt caattttaag cccaaaggga tttcttgtta aagttacaat ctctgctaga
    10981 gggagagcct cgtctaagcg taagcccgct aacccgaagg catgagctcg ttcaataagc
    11041 tgatctgacc aaccagcttg gctaataaag cttggactaa cgtcagcaat tactcgaatg
    11101 cctaattgat ttgcataagc aatgagctct gcgtagcaat gaaacatctg gtgatcagct
    11161 ggtgccagct gtaagagact cataaacaag cgcttggctc catagctatg acataagtca
    11221 atataagctt tactcttggt aacatcatac cgctcagggt atagagaaaa tcctaaatca
    11281 accatattgc ctcctattta tcaaaaccat ttgactcgct caacgcctta aaccagtaac
    11341 ctgatttttt tatggttcgc ttttgactct ctaaatccaa agctaccaaa ccatatcggt
    11401 ttttataagc atttaaccaa gaccagcaat caataaaagt ccacaagagg tagcctttgc
    11461 agttggctcc ttctcccaaa gccttatgaa gctggataag atgatcctcg ataaaggtga
    11521 tgcggtagtc atcttgtatc tggccatctg ctaaaaaagc ttcttcacct tcgacaccca
    11581 taccattttc cgtgactagc cattcgatgt taccatagtg ttcttttaaa ttaagggcaa
    11641 tatcataaag tcctggttca taaatttccc aaccccggtg aggattgact tttttaccag
    11701 gcatatcata aggttcaaaa taaatatcta agataagggg gtccccctct tgctgtgact
    11761 tactaggtgc ctgaacccgc aaaggctgat aataattaac tcccaaaaaa tcaacagtat
    11821 tgtttttgat aacctctaat tcatcagcac tatattgagg taataaatca gcttcctcta
    11881 aaatggaaac taactctgct gggtaaacac ccaaaactga tggatctagg aaggatttgg
    11941 tttggaaaag ctctgcgatt ctagcagcct tgacatcttc aggagcatca ctccgaggat
    12001 aagcaggtgt catgttaagc acaatactaa tcttgtggtc gggatgaagc ttatgacaag
    12061 ctttaaccgc cagtgaactg gccagttgtg tgttgtaagc cacttgaact gctgccttgg
    12121 catctacttt acaagggtag tgataataac ctaaatagcc acattcgact ggaacaatcg
    12181 gttcattaaa ggtaatccaa gtgtttacta aatcaccaaa aagctcaaaa caggtttttg
    12241 cgtaggtttc ataagcccag acagtagctt tgttttccca gccacctttt tcttgcaagg
    12301 cataggggag gtcaaaatga tagaggttaa caatcaattt aataccttga gcaataatgt
    12361 cttgaaatac ttctcggtaa aatgtcaccg cctttggatt aacctccccg actccttctg
    12421 gaatcaatcg agaccactgg attgaggtcc gaaaaatcgt gtggcctgtt tctttcaaaa
    12481 gggcaatgtc acccttatag ttttcataaa aggttgaggt tttttctgga ccaatttggt
    12541 gatgaaactt agctgactca atactaaacc agtaatccca gttgctaggt cctttgccat
    12601 ctccaggaac cgttccttcg ctttgaggcc cagatgtgga acttccccat aaaaagccat
    12661 ctggaaattg ataacggtgt tttctttgtt tcatatcctt tgtcctttct ttgctcactt
    12721 ctctttttat tataagaaga aaaacaggtg atgtaaacgc tttaaaaaat ctgttttttc
    12781 cttatcatca cggaaaataa gtgatttaaa gcttgacata ttttattatc ttaaagagcc
    12841 tatctaacct gccctgctac ttcttatcaa aaccaagccc ctattgacag atgccttagc
    12901 ctatgtttga ataggggtat ggcttattta aactacttta caaaaaccga gtgggcactt
    12961 tggttaagct ctgtcttggc cattttaatt agcgcccttt tattcggtaa gcaagctccc
    13021 ttagctttaa tagcttcttt gattggtgtg acctctctca tttttagtgc taaagctaat
    13081 cctattggtc aagggctcgt cattattttt tccattatct atgcctatct ttcattacga
    13141 aacagttact atggagagtt aatgacctat cttttcatga ccctccctat gactattttt
    13201 tcacttttta cttggttaaa ccatcctttt gaagggaaaa aatcacaagt gactatttca
    13261 cgattaacac ccactgatcg aagatgtctt ttcgttttta ctatactggt cactcttatt
    13321 ttctacggca tcttggcttc tattcaaaca gcctatttac ttgtctccac tttatcgatt
    13381 gccacttctt tctcagccgt ttatctcagc tataagcgaa gcccttattt tgctttggct
    13441 tatggactca atgatctagt cctcattctc ttatggatgc atgctgctca aacagatgcc
    13501 agccaatatg ctgtggtcat ctgctttgcc acctttctta ttaatgatag ttacactttt
    13561 tatagttggc taatgctcca acgttaccaa gaaaaaaagg tcaaaaaagg atgagggcct
    13621 aagcctcatc ctttttcaat ttttcacgtt ctaagcgcaa acgtctattg ggatttaaaa
    13681 gattaaacaa ttcttctaat ttctcagcat tccaaacatc tgctgccgat acaaaatttc
    13741 catccttatc cttaaaggat atcggtttat cacccataat cagaaacctc catcttaaag
    13801 ataaaacaca aggctgctat ggcaatgcca tttctaaaga ccacattaat ccacaaattc
    13861 aaattcaaat ttgccaatac gaacaagatc cccgtctttt gctccacgtt cacgaagggc
    13921 ttcgtcaacc cccataccgc gcaattgacg ggcaaatttc atgatggatt cgtcacgttc
    13981 catattggtc atgacaaaga gacgttctaa cttctcacca gacaagaccc aagtagcgtc
    14041 atcgtcacgt gtgatctcaa attctttctc agcctcagca aaaccatagt aagcttcctc
    14101 gtccaccaga tcagattcat catacaataa gaattcatcc gtcttagcaa gtaattctgc
    14161 cgttgcttct aacaagttct ctaagccttg atgtgctagg cttgaaatag ggaaaatcat
    14221 tggcaaatca tcaaactcgt catattgggc agccaatttt ttcttgaagg ctttcaagtt
    14281 ttcttgagcc tctggcatgt ccattttatt agccacaata atctgtgggc gctccatcaa
    14341 tcgcaaatta taggtttcca gctcgttatt aatagaaaca taatcttcgt aaggatcacg
    14401 accctcacta gcagacatgt caatcacatg aagaataacc cgtgtccgct cgatatgacg
    14461 gaggaattga gttcccaaac caaccccttg gctagcacct tcaatcaaac ctggcagatc
    14521 tgccatagca aagctatcac ctgactttgt acgaaccatt ccaagattag gaacaatagt
    14581 ggtaaaatga taggcaccaa ttttcggttt agctgatgac acaacactta acaaggttga
    14641 tttcccaaca gatgggaatc cgaccaagcc aacgtcagct aagattttga gttctaactc
    14701 taactgacgt tcctcacctg gctcaccatt ttcagcaatc tctggcgcag gattacgagg
    14761 agttgcaaaa cggatattcc cacggccacc acgaccaccc ttagcaatga ccacttcttg
    14821 accatgttct actaagtctg taattacttt tccagtttca gcatcacgaa ccgttgtccc
    14881 ttgaggaaca aaaacaatca aatcttctgc accacggccg tgcatgcctt tggtcatacc
    14941 tttttcacca gatttcgcct tgaatttacg attatagcgg aaatccatca aggtacgcaa
    15001 accttcgttc actcggaaga taactgagcc acctttaccg ccgtctcctc cccaaggacc
    15061 gccatttggg acgtactttt cacgacgaaa agctaccata ccatcgccac cacggcctgc
    15121 ttggacactg atttttgccg tatctaaaaa catactcata ctttttttat tctctaactt
    15181 tcttttactt taaaaaaacg cctttcagcg tttccttaca ttaatactga tagggcactc
    15241 gcaataagcc cgccaacagt tacaagaacc attaggataa caacgaccat tgtcactttt
    15301 tcaaaggttg tttttttgcg aggtccattt tctccaaatg ccacgttaaa tacctcatct
    15361 attgattttt cttcttatat cttaacatga aagatcgttt taggcaaata ccagatgaat
    15421 atttacgcta tgtaggctat tactgagtat tgtatcttgt gaatttcata tgaatatgat
    15481 acattagcat ttgcaaaata atgttacaga taaaaacctc ctcgtctg
//
LOCUS       DATXIG010000036        14923 bp    DNA     linear   BCT 28-DEC-2023
DEFINITION  TPA_asm: Streptococcus pyogenes strain MGAS11113
            SAMN08555799-rid19682583.denovo.036, whole genome shotgun sequence.
ACCESSION   DATXIG010000036 DATXIG010000000
VERSION     DATXIG010000036.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN08555799
            Sequence Read Archive: SRR6740495
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pyogenes
  ORGANISM  Streptococcus pyogenes
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 14923)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14923)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-OCT-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-OCT-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001918058.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001918058.2
            Genome Coverage       :: 368x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/12/2023 11:23:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 1,806
            CDSs (total)                      :: 1,769
            Genes (coding)                    :: 1,722
            CDSs (with protein)               :: 1,722
            Genes (RNA)                       :: 37
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1, 1 (5S, 23S)
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 47
            CDSs (without protein)            :: 47
            Pseudo Genes (ambiguous residues) :: 0 of 47
            Pseudo Genes (frameshifted)       :: 30 of 47
            Pseudo Genes (incomplete)         :: 20 of 47
            Pseudo Genes (internal stop)      :: 9 of 47
            Pseudo Genes (multiple problems)  :: 11 of 47
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14923
                     /organism="Streptococcus pyogenes"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN08555799-rid19682583.denovo.036"
                     /strain="MGAS11113"
                     /serotype="Emm28"
                     /isolation_source="normally sterile site"
                     /host="Homo sapiens"
                     /db_xref="taxon:1314"
                     /country="Finland"
                     /collection_date="2002"
                     /collected_by="National Public Health Laboratory"
     gene            35..739
                     /locus_tag="VC122_001193"
     CDS             35..739
                     /locus_tag="VC122_001193"
                     /EC_number="6.3.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017648569.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylaminoimidazolesuccinocarboxamide
                     synthase"
                     /protein_id="HEP5641118.1"
                     /translation="MTNQLIYKGKAKDIYSTKDENVIRTVYKDQATMLNGARKETIDG
                     KGALNNQISSLIFEKLNKAGVVTHYIEQISKNEQLNKKVDIIPLEVVLRNVIAGSFSK
                     RFGVEEGRVLETPIVEFYYKNDDLNDPFINDEHVKFLGIVNDEEIAYLKGETRRINEL
                     LKGWFAQIGLNLIDFKLEFGFDQEGTIILADEFSPDNCRLWDKNGNHMDKDVFRRDLG
                     NLTDVYQVVLEKLIAL"
     gene            863..4577
                     /locus_tag="VC122_001194"
                     /pseudo
     CDS             863..4577
                     /locus_tag="VC122_001194"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001042262.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylformylglycinamidine synthase"
     gene            4738..6192
                     /locus_tag="VC122_001195"
     CDS             4738..6192
                     /locus_tag="VC122_001195"
                     /EC_number="2.4.2.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016480155.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidophosphoribosyltransferase"
                     /protein_id="HEP5641119.1"
                     /translation="MTYEVKSLNEECGVFGIWGHPQAAQVTYFGLHSLQHRGQEGAGI
                     VSNDNGKLYGYRNVGLLSEVFKNQSELDNLTGNAAIGHVRYATAGSADIRNIQPFLYK
                     FHDGQFALCHNGNLTNAISLRKELEKQGAIFNASSDTEILMHLIRRSHNSSFMGKVKE
                     ALNTVKGGFAYLLMTENKLIAALDPNAFRPLSIGQMQNGAWVISSETCAFEVVGAKWV
                     RDVEPGEVILIDDRGIQCDRYTDETQLAICSMEYVYFARPDSTIHGVNVHTARKNMGK
                     RLAQEFKQDADIVIGVPNSSLSAAMGFAEESGLPNEMGLVKNQYTQRTFIQPTQELRE
                     QGVRMKLSAVSGVVKGKRVVMIDDSIVRGTTSRRIVGLLREAGASEVHVAIASPELKY
                     PCFYGIDIQTRRELISANHAADEVCDIIGADSLTYLSLDGLIESIGLETKAPNGGLCV
                     AYFDGHYPTPLYDYEEEYLRSLEEKTSFYIQKVK"
     gene            6220..7242
                     /locus_tag="VC122_001196"
     CDS             6220..7242
                     /locus_tag="VC122_001196"
                     /EC_number="6.3.3.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016480156.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylformylglycinamidine cyclo-ligase"
                     /protein_id="HEP5641120.1"
                     /translation="MSEKNAYAKSGVDVEAGYEVVERIKKHVARTERAGVMGALGGFG
                     GMFDLSKTGVREPVLVSGTDGVGTKLMLAIKYDKHDTIGQDCVAMCVNDIIAAGAEPL
                     YFLDYVATGKNNPVKFEEVVSGVAEGCVQAGAALIGGETAEMPGMYGEDDYDLAGFAV
                     GVAEKSQLIDGSKVKEGDILLGLASSGIHSNGYSLVRRVFADYTGKELLPELEGKQLK
                     DVLLEPTRIYVRAALPLIKEELINGIGHITGGGFIENVPRMFADDLAAEIDEDKVPVL
                     PIFKALEKYGDIKHEEMFEIFNMGVGLMLAVSPENVNRVKELLDEPVYEIGRIIKKAD
                     DSVVIK"
     gene            7410..7964
                     /gene="purN"
                     /locus_tag="VC122_001197"
     CDS             7410..7964
                     /gene="purN"
                     /locus_tag="VC122_001197"
                     /EC_number="2.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002987709.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylglycinamide formyltransferase"
                     /protein_id="HEP5641121.1"
                     /translation="MKIAVFASGNGSNFQVIAEQFPVSFVFSDHRDAYVLERAQNLAI
                     PSFAFELKEFENKAAYEQAIVDLLDKHEIDLVCLTGYMKIVGETLLLAYEGRIINIHP
                     AYLPEFPGAHGIEDAWEAGVDQSGVTIHWVDSGVDTGQVIQQVRVPRLADDSLESFET
                     RIHETEYQLYPAVLDSLGVERKVI"
     gene            8148..9695
                     /gene="purH"
                     /locus_tag="VC122_001198"
     CDS             8148..9695
                     /gene="purH"
                     /locus_tag="VC122_001198"
                     /EC_number="2.1.2.3"
                     /EC_number="3.5.4.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006146083.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional
                     phosphoribosylaminoimidazolecarboxamide
                     formyltransferase/IMP cyclohydrolase"
                     /protein_id="HEP5641122.1"
                     /translation="MTKRALISVSDKSGIVDFAKELKNLGWDIISTGGTKVALDNAGV
                     ETIAIDDVTGFPEMMDGRVKTLHPNIHGGLLARRDADSHLQAAKDNNIELIDLVVVNL
                     YPFKETILRPDITYDLAVENIDIGGPSMLRSAAKNHASVTVVVDPADYATVLGELADA
                     GQTTFETRQRLAAKVFRHTAAYDALIAEYFTTQVGEAKPEKLTITYDLKQAMRYGENP
                     QQDADFYQKALPTDYSIASAKQLNGKELSFNNIRDADAAIRIIRDFKDRPTVVVLKHM
                     NPCGIGQADDIETAWDYAYEADPVSIFGGIVVLNREVDAATAKKMHPIFLEIIIAPSY
                     SEEALAILTNKKKNLRILELPFDAQAASEVEAEYTGVVGGLLVQNQDVVAENPSDWQV
                     VTDRQPTEQEATALEFAWKAIKYVKSNGIIITNDHMTLGLGAGQTNRVGSVKIAIEQA
                     KDHLDGAVLASDAFFPFADNIEEIAAAGIKAIIQPGGSVRDQDSIDAANKHGLTMIFT
                     GVRHFRH"
     gene            complement(9753..10877)
                     /locus_tag="VC122_001199"
     CDS             complement(9753..10877)
                     /locus_tag="VC122_001199"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011054094.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SH3 domain-containing protein"
                     /protein_id="HEP5641123.1"
                     /translation="MKKFHRFLVSGVILLGFNGLVPTMPSTLISQQENLVHAAVLGDN
                     YPSKWKKGNGIDSWNMYIRQCTSFAAFRLSSANGFQLPKGYGNACTWGHIAKNQGYPV
                     NKTPSIGAIAWFDKNAYQSNAAYGHVAWVADIRGDTVTIEEYNYNAGQGPERYHKRQI
                     PKSQVSGYIHFKDLSSQTSHSYPRQLKHISQASFDPSGTYHFTTRLPVKGQTSIDSPD
                     LAYYEAGQSVYYDKVVTAGGYTWLSYLSFSGNRRYIPIKEPAQSVVQNDNTKPSIKVG
                     DTVTFPGVFRVDQLVNNLIVNKELAGGDPTPLNWIDPTPLDETDNQGKVLGDQILRVG
                     EYFTVTGSYKVLKIDQPSNGIYVQIGSRGTWVNADKANKL"
     gene            11130..12395
                     /gene="purD"
                     /locus_tag="VC122_001200"
     CDS             11130..12395
                     /gene="purD"
                     /locus_tag="VC122_001200"
                     /EC_number="6.3.4.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016502383.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylamine--glycine ligase"
                     /protein_id="HEP5641124.1"
                     /translation="MKLLVVGSGGREHAIAKKLLASKGVDQVFVAPGNDGMTLDGLDL
                     VNIVVSEHSRLIAFAKENEISWAFIGPDDALAAGIVDDFNSAGLRAFGPTKAAAELEW
                     SKDFAKEIMVKYNVPTAAYGTFSDFEKAKAYIEEQGVPIVVKADGLALGKGVVVAETV
                     EQAVEAAQEMLLDNKFGDSGARVVIEEFLDGEEFSLFAFVNGDKFYIMPTAQDHKRAF
                     DGDKGPNTGGMGAYAPVPHLPQSVVDTAVETIVRPVLEGMVAEGRPYLGVLYVGLILT
                     ADGPKVIEFNSRFGDPETQIILPRLTSDFAQNIDDIMMGIEPYITWQKDGVTLGVVVA
                     SEGYPLDYEKGVPLPEKTDGDIITYYAGAKFVENSELLLSNGGRVYMLVTTEDSVKAG
                     QDKIYTQLAQQDTTGLFYRNDIGSKAIRE"
     gene            12673..13164
                     /gene="purE"
                     /locus_tag="VC122_001201"
     CDS             12673..13164
                     /gene="purE"
                     /locus_tag="VC122_001201"
                     /EC_number="4.1.1.21"
                     /EC_number="5.4.99.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001875905.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5-(carboxyamino)imidazole ribonucleotide mutase"
                     /protein_id="HEP5641125.1"
                     /translation="MKTPIISIIMGSKSDWATMQKTAEVLDNFGIAYEKKVVSAHRTP
                     DLMFKHAEEARGRGIKIIIAGAGGAAHLPGMVAAKTTLPVIGVPVKSRALSGLDSLYS
                     IVQMPGGVPVATMAIGEAGATNAALTALRILSIEDQNLADALAHFHEEQGKIAEESSG
                     ELI"
     gene            13151..14224
                     /gene="purK"
                     /locus_tag="VC122_001202"
     CDS             13151..14224
                     /gene="purK"
                     /locus_tag="VC122_001202"
                     /EC_number="6.3.4.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001084009.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5-(carboxyamino)imidazole ribonucleotide
                     synthase"
                     /protein_id="HEP5641126.1"
                     /translation="MNSFKTIGIIGGGQLGQMMAIAAIYMGYKVITLDPASDCPASRV
                     SEVIVAPYDDVEALGQLAARCDVLTYEFENIDADGLDAVVSACQLPQGTDLLRISQNR
                     IFEKDFLANKAGVTVAPYKVVTSSLDLGGLDFTKTYVLKTATGGYDGHGQKVIRSAED
                     LPEAQQLANSAQCVLEEFVNFDLEISVIVSGNGEDVTVFPVQENIHCNNILSKTIVPA
                     RISDQLADKAKKMAVQIAKKLQLSGTLCVEMFATADDIIVNEIAPRPHNSGHYSIEAC
                     DFSQFDTHILGVLGAPLPTIHLHAPAVMLNVLGQHVQQAIDYVAQNPSAHLHMYGKLE
                     AKHNRKMGHVTVFSDLPDEVEEF"
     gene            14226..>14923
                     /locus_tag="VC122_001203"
     CDS             14226..>14923
                     /locus_tag="VC122_001203"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011888524.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEP5641127.1"
                     /translation="MYLKYIFKIKDRNLVSDYESLVRNLTINLQKLVIKDRTALIGDK
                     QLPITIYYRGENEKFPTGIVKVESDSVEGLELFDKTIKEIVGNESRFGKFTCIYDGIS
                     TYYSTEIYSELHEIERRTQEFLREAFFLSDKSGELKEYQATIKKMDSSDFSFSKLINE
                     IFKASDITDTFIDHVRACVEFGTVPNGDLLPKSFWDSLDDSLLDDKEQGLEINNLLNE
                     IRSTRNTIAHCKEF"
BASE COUNT         4290 a         2896 c         3500 g         4237 t
ORIGIN      
        1 ttaaatgaaa acagaaagaa aggcgaataa tcaagtgact aaccagctta tttataaagg
       61 aaaagcgaaa gatatttata gtacaaaaga tgaaaatgtg attagaacgg tctataaaga
      121 ccaagctact atgttgaatg gagctcgtaa ggagactatt gatggcaagg gagcactaaa
      181 taatcagatt tcatctctta tttttgagaa attaaataag gctggtgtgg tgacacatta
      241 tattgagcaa atttcaaaaa acgaacaatt gaataaaaaa gttgatatta ttccacttga
      301 ggtggtgtta cggaatgtga tagcaggctc attttcaaaa cgttttggag tggaagaagg
      361 acgtgttctt gaaacgccaa ttgtggaatt ttactacaaa aatgatgatc tgaatgatcc
      421 ttttatcaat gatgagcatg tgaagttttt gggtattgtt aatgatgagg agattgctta
      481 tctgaaagga gagactcgcc gtattaatga actcttgaaa ggttggtttg ctcagattgg
      541 gcttaacttg attgacttca agttggagtt cggatttgat caagaaggta caattatctt
      601 agcagatgaa ttttcgcctg ataactgtcg cctatgggat aagaatggta accatatgga
      661 caaggatgtt tttagaagag accttggcaa cctgacggat gtctatcaag ttgtacttga
      721 gaagttgata gcattgtaac tagaagagga aggaattgaa tccgttcggg aaactccgaa
      781 aaaaagatgg aacttactga aatctcaatt tcagatgtcc tcctattttc cagtcgctcc
      841 cttgtcggac ttggtatctt atatgaataa acgtattttt gttgagaaaa aggctgactt
      901 tggtattaaa tcggctagcc ttgtgaaaga gttgacgcat aatctacaac tgacttcttt
      961 gaaggctttg cgtattgtgc aggtctatga tgtcttcaat ttggctgagg atttgctggc
     1021 gcgtgctgag aagcatattt tctctgagca ggtgacagac tgtcttttga cggaaactga
     1081 aatcactgcg gagcttgata aggttgcctt ctttgccatt gagtcgcttc ctggtcaatt
     1141 tgaccaacgt gctgttagtt cgcaagaagc tttgctatta tttggaagtg acagtcaggt
     1201 taaggtcaat acagcccagc tttacttggt caataaggat attgcagaag cagagcttga
     1261 agccgttaag aactatcttt tgaaccctgt tgattcgcgt ttcaaggaca ttactttgcc
     1321 gcttgaagag caggctttct ctgtgtctga taagacgatc cctaatcttg atttcttcga
     1381 gacttataag gctgacgatt ttacggctta taaggtagag cagggcttgg ctatggaggt
     1441 cgatgatctt ctcttcatcc aagattattt caaatcaatc gggcgtgtgc caactgagac
     1501 tgagttgaaa gttttggata cgtattggtc agaccactgt cgtcacacaa cctttgaaac
     1561 tgaattgaag aacattgatt tttcagcttc taaattccaa aaacaattgc agacaactta
     1621 tgacaaatat atcgccatgc gtgatgagct tggtcgttct gaaaagccac aaacacttat
     1681 ggatatggcg actatttttg gtcgttatga gcgtgccaac ggtcgtctgg acgatatgga
     1741 agtctcagat gaaatcaatg cctgctcagt tgagattgaa gtagatgttg atggtgtgaa
     1801 agagccttgg ctcctcatgt ttaagaatga gacacacaat cacccaacag aaattgagcc
     1861 attcggtgga gcggcgactt gtatcggtgg tgctattcgt gacccattgt caggacgttc
     1921 atacgtttat caggctatgc gtatttcagg cgcaggcgat atcacgactc cgattgcgga
     1981 aacacgtgct ggtaaattgc cacaacaggt tatttctaag actgcggcgc acggctattc
     2041 ttcatatggt aaccaaattg gacttgcgac aacttatgtg cgtgagtact tccaccctgg
     2101 cttcgtagcc aaacgtatgg agcttggagc tgtggttggt gctgcaccta aggaaaatgt
     2161 ggttcgtgaa aaaccagaag caggtgatgt ggtaatcttg cttggtggta aaacagggcg
     2221 tgacggtgtc ggcggtgcga caggttcatc taaggttcaa acggttgaat ctgtggaaac
     2281 agcaggagca gaggtacaaa aagggaatgc catcgaagag cgtaagattc aacgtctttt
     2341 ccgtgctggc aatgtcactc gtctgatcaa gaaatcaaat gacttcggtg caggtggtgt
     2401 ctgtgttgcc atcggtgaat tggctgacgg tcttgaaatc gatttggaca aggtgcctct
     2461 taaataccaa ggtcttaatg gtactgaaat tgcaatctca gaatctcaag agcgtatgtc
     2521 agtcgttgtt cgtccaagtg atgtggatgc cttcatcgca gcctgcaaca aggaaaatat
     2581 cgatgcagtc gtagttgcga ctgtgactga aaaaccaaat cttgtcatga cttggaatga
     2641 tgaaacaatt gttgatttgg aacgccgttt ccttgatacc aatggtgtcc gtgtcgttgt
     2701 tgatgctaaa gtcgttgaca aggacttgac agttccagaa gcacgcataa catcagcaga
     2761 gacacttgaa gcagatacgc ttaaggtctt gtctgacctc aaccatgcta gtcaaaaagg
     2821 tctccaaacc atctttgact catctgttgg tcgttcaacc gttaaccacc caatcggtgg
     2881 tcgttaccaa atcacaccga cagaaagttc tgttcaaaaa ttgccagttc aacatggtgt
     2941 gacaacaact gcatctgtta tggctcaagg ttacaatgct tatattgcag agtggtcacc
     3001 ttatcacggt gctgcctatg ctgttattga agcgacagct cgcttggtag caacaggtgc
     3061 tgactggtct cgtgcacgtt tctcttacca agagtatttc gagcgtatgg ataaacaggc
     3121 agagcgtttt ggtcagccag tatcagctct tcttggttct attgaggctc agattcaact
     3181 tggcttgcca tcaatcggtg gtaaggactc tatgtctggt actttcgaag acttgacagt
     3241 accaccaacc ttggtagctt tcggtgtgac aacagcggac agccgcaaga ttctctctcc
     3301 tgagtttaaa gcggctggtg agaatatcta ctacattcca ggtcaagcta tttcagaaga
     3361 tattgatttt gaccttatca aggctaactt cagtcagttt gaagctattc aaactcaaca
     3421 taagattaca gctgcctcag ccgttaaata cggtggtgtc ctagaaagtt ttgctctcat
     3481 gacttttggt aaccgtatcg gtgcttctgt tgaaattgca gagcttgaca gctcaactcg
     3541 gaggttttgt ctttacatca gctgaggaaa ttgctgacgc ggtgaaaatt ggtcaaactc
     3601 aggcagactt tacagtcact gtcaatggaa atgaccttgc tggcgctagc cttctagcag
     3661 ccttcgaagg caaattggaa gaggtttacc caacagaatt tgaacagaca gatgttcttg
     3721 aagaagttcc tgctgtggta tcagatactg ttatcaaggc taaggaaaca attgaaaaac
     3781 cagtggttta cattccagtc ttccctggta ccaactcaga gtacgattca gctaaggcct
     3841 ttgaacaggt tggagctagt gtcaacttgg taccatttgt aaccttgaat gaggttgcta
     3901 ttgctgagtc agttgacact atggttgcta atattgctaa ggcaaatatc atcttctttg
     3961 ctggaggttt ctcagcagcg gatgaaccag atgggtctgc taagtttatc gtcaatatct
     4021 tgcttaacga gaaggtccgc gcagctattg acagcttcat cgaaaaaggt ggccttatca
     4081 tcggtatctg taatggtttc caagcccttg ttaaatcagg tcttcttcca tacggaaact
     4141 tcgaggaagc tggtgagaca agtccaactc tcttctataa cgatgctaat cagcatgttg
     4201 ccaagatggt tgagactcgt atcgcaaata ccaactcacc ttggttggca ggagttgagg
     4261 tcggcgatat tcatgccatt ccagtttcac atggtgaagg taaatttgtt gtcagcgctt
     4321 ctgaatttgc agagctaaga gacaatggtc aaatctggag ccaatatgtg gactttgacg
     4381 gacaaccatc tatggattct aaatacaatc caaacggctc tgtcaatgcc atcgaaggga
     4441 ttaccagcaa gaatggtcaa atcatcggta agatgggaca ctcagaacgc tgggaagacg
     4501 gactcttcca aaatattcct ggtaacaaag accaaaccct ctttgcaagt gctgtaaaat
     4561 actttacagg gaagtaaaaa ggttcagtga acctttttag ccctcgcctt gaattagtga
     4621 agggcaaggt tagaaagaat ctcgcttaat ttttgagaaa tagtaaacac gggcgataag
     4681 ttgtgcgaaa aactttctat tttcgcttta cttgacagcc tttgtatcga ggtaaaaatg
     4741 acatacgaag taaaatctct aaatgaagaa tgtggagtct ttggtatctg gggacatcct
     4801 caggcagctc aagtcactta ctttgggctt catagccttc aacatcgcgg tcaagaagga
     4861 gctggtattg tttcaaatga caatgggaaa ctctatggtt atcgaaatgt tgggctcctt
     4921 tctgaagttt ttaagaatca atctgaatta gataatttaa ctgggaatgc ggctattgga
     4981 catgttcggt atgctactgc aggttctgca gatattcgca atattcagcc ttttctttat
     5041 aaatttcatg acgggcaatt tgctttatgc cataatggta atttgacaaa tgctatttcc
     5101 ttaaggaaag aattagaaaa gcaaggtgca attttcaatg cctcctcaga tactgaaatt
     5161 ttgatgcact tgattcgtcg aagccataac tcaagtttca tgggaaaggt aaaagaagct
     5221 ctaaatacag taaagggtgg ctttgcctat ctactgatga cagaaaataa actcattgct
     5281 gctcttgacc ctaatgcctt tcgtcctttg tcaattggac aaatgcaaaa tggtgcctgg
     5341 gttatttcca gtgagacctg tgcttttgag gtggtaggcg caaaatgggt tagagatgtt
     5401 gaacctggcg aagttattct tatagacgat cgtggtattc aatgtgatcg ttatactgat
     5461 gaaacgcaac tggcaatttg ttcgatggaa tatgtctatt ttgcaaggcc ggattcaact
     5521 atacatggtg ttaatgttca tacggctcga aaaaatatgg gaaagcgtct tgcacaagaa
     5581 tttaaacagg atgctgatat tgtaattggt gtcccaaatt cgtccttatc ggctgctatg
     5641 ggctttgctg aagaatccgg attaccaaat gagatgggtc ttgtaaaaaa tcagtatacg
     5701 cagcgaacct ttattcaacc gacacaagaa ttaagggaac aaggtgttcg aatgaaacta
     5761 tcagcggtat ctggtgttgt caaaggaaag cgcgttgtta tgattgatga ctcaattgta
     5821 agaggaacga cttctagaag gattgtcgga ttattaagag aagcaggagc tagtgaagta
     5881 catgttgcta tagctagtcc agaattaaag tatccttgtt tttatggtat tgatattcaa
     5941 acaagacgag aattaatttc agcaaatcac gctgctgacg aagtgtgtga tattattggc
     6001 gcagatagtc tgacctatct ctcactagat gggttgattg aatcaattgg actcgaaaca
     6061 aaagcaccaa atggtggttt atgcgtagct tactttgatg gacattatcc aacaccgctt
     6121 tatgattatg aagaagaata cctcagaagt ttagaagaaa aaacaagttt ttatattcaa
     6181 aaagtgaaat aagctctgct aattatataa ggagaaaaca tgtctgaaaa aaatgcttat
     6241 gcaaaatctg gtgttgatgt tgaagctggt tatgaagtgg ttgaaagaat aaaaaaacac
     6301 gttgctcgta cagaacgtgc gggagtcatg ggagctctag gtggctttgg tgggatgttt
     6361 gacttgagta aaacaggagt tagagagcct gttttggtct cagggacaga cggtgttggc
     6421 acaaaactca tgcttgctat caagtacgac aagcacgaca caatcggtca agactgtgtt
     6481 gccatgtgtg tcaatgatat tattgcagca ggtgctgagc ccctttactt ccttgactac
     6541 gtagcgacag gaaaaaataa tccagtcaaa tttgaagaag ttgtttctgg cgttgcagaa
     6601 ggttgtgttc aagcaggagc agcactcatt ggaggtgaga ccgctgaaat gcctgggatg
     6661 tatggcgaag atgattatga ccttgctggc tttgctgttg gtgtggctga aaaatctcag
     6721 ctcatcgacg gttcaaaagt aaaggaaggc gatattcttt tgggacttgc ttcaagtggt
     6781 atccattcaa acggttattc tttggtacgt cgtgtctttg ctgactatac tggtaaagag
     6841 ctgcttccag aacttgaagg aaaacaactt aaggatgtcc ttcttgagcc aactcgtatt
     6901 tatgttagag cagctctgcc attaatcaag gaagaactaa tcaacggtat cggccacatc
     6961 acaggtggtg gctttatcga gaatgttcct cgtatgtttg cagatgattt ggctgcggaa
     7021 atcgatgagg acaaggtgcc agtgcttccg attttcaagg cgcttgaaaa atatggtgac
     7081 atcaagcacg aagaaatgtt tgaaatcttc aatatgggtg tcggtcttat gctagctgtt
     7141 agtcctgaaa atgttaaccg cgtcaaagag ctcttggatg aaccagttta tgaaatcggt
     7201 cgtatcatca agaaagcaga cgatagtgtg gtgattaaat aagtcactaa attttataag
     7261 aaaggagtgt ttagttagtt tgcctaactg aacacgggat taaatttcta ggaaaaaaga
     7321 taaaatgact tagacgcaag cgtcgttgtt cgttttccta tttttctacg aaatttttcg
     7381 gcaagccgaa tcatcccttt gtatcgaata tgaaaatcgc tgtttttgct tctggtaatg
     7441 gttccaactt tcaggtcata gcagagcagt ttccagttag ttttgtcttt tcagatcatc
     7501 gtgatgccta tgttttagag cgtgctcaga atttagccat tccaagcttc gcttttgaac
     7561 tcaaggagtt tgagaataag gcggcttacg agcaagccat cgttgatttg ttggacaaac
     7621 acgagattga tttggtctgt ctaacgggct atatgaagat tgttggagaa accttactct
     7681 tggcctatga ggggcgtatt atcaatattc acccagccta cctgcctgaa tttccaggtg
     7741 cccacggtat cgaggatgct tgggaagctg gtgttgacca gtctggcgtg accatccact
     7801 gggttgactc tggtgtggat accggtcagg tcatccaaca agtgcgcgtg ccacgcctag
     7861 cagacgatag cctagaaagc tttgaaaccc gtatccatga aaccgaatat caactctacc
     7921 cagctgtctt agatagcttg ggagtggaga ggaaagtaat ctagccttta tcttaaaatt
     7981 ttaggtaaag ttaggaaaga tagtaaagaa gaattaacgt tccaaaaaga aaggaattga
     8041 acccgtctgg aaggcacaga aaaaagataa acatctccta ggcgcaagcg tcttcgtcga
     8101 gtttcctatt tttccttggc cttctagtcg gacttggtat cttaaatatg actaaacgtg
     8161 ctttaatctc agtgtctgac aagtcaggaa ttgttgactt tgcaaaagaa ttgaaaaact
     8221 tgggttggga tattatctca actggtggga ctaaggttgc ccttgataat gctggcgttg
     8281 agaccattgc catcgacgat gtgactggat tcccagagat gatggacggt cgtgttaaga
     8341 ccctccaccc aaacattcac ggtgggcttt tggctcgtcg cgacgctgac agccaccttc
     8401 aggctgctaa ggacaacaat atcgagctga ttgacctcgt ggttgtcaac ctctatccct
     8461 tcaaggagac tatccttcgt ccagatataa cctatgattt ggcggtggaa aacatcgaca
     8521 tcggcggtcc atcaatgctt cgctcagccg ctaaaaatca cgctagcgtg accgttgtgg
     8581 ttgacccagc ggattatgcg actgttttgg gagaattggc tgacgctggt cagacgacat
     8641 ttgaaacgcg tcaacgcttg gcagctaagg tctttcgtca cacagcagcc tacgacgctt
     8701 tgattgctga gtacttcaca actcaagtgg gagaggctaa gcctgaaaaa ttaaccatca
     8761 cttacgacct taaacaggct atgcgttacg gagaaaatcc acaacaagac gctgatttct
     8821 atcaaaaagc cttgccaaca gactactcaa ttgcttctgc taaacagctc aacggaaaag
     8881 aattgtcctt caataatatc cgtgatgccg atgcagcaat ccgtattatc cgtgatttca
     8941 aagatcgtcc aactgttgtt gtcctcaaac acatgaaccc atgtggtatc ggacaggctg
     9001 atgatattga gacagcttgg gattacgctt atgaagctga cccagtttca atctttggtg
     9061 gaattgtcgt ccttaaccgt gaagttgacg cagcgacagc taagaagatg caccctatct
     9121 tcttggaaat catcatcgca ccatcatact cagaagaagc gctagctatt ctcacaaata
     9181 aaaagaaaaa cttgcgaatc cttgagttgc cgtttgatgc ccaagctgcc agcgaagtgg
     9241 aagctgagta cactggcgta gttggtggac ttttggtgca aaaccaagac gttgtggctg
     9301 aaaatccatc tgactggcaa gtggtgacag accgccaacc aacagaacaa gaggcgactg
     9361 cccttgagtt tgcctggaag gctatcaagt atgttaagtc taacgggatt attattacta
     9421 acgatcacat gacgcttgga ctcggtgcag gtcaaaccaa ccgtgtcggc tcagtcaaga
     9481 ttgctatcga gcaggctaaa gaccaccttg acggtgccgt tctagcatca gatgccttct
     9541 tcccatttgc ggacaacatt gaagaaatcg ctgccgcagg gatcaaagca atcatccaac
     9601 caggtggttc agttcgtgac caagactcta ttgacgccgc aaacaaacat ggcttgacca
     9661 tgatcttcac aggtgtgaga cattttaggc attaataaaa aaatgtggtc taattaaaac
     9721 cacatttttt attaaaagaa aatataagtt aactataatt tgttagcttt atcagcattt
     9781 acccatgttc cacgagatcc gatttgaaca taaataccat tacttggttg atcaattttt
     9841 aatactttat aactaccagt gacggtaaaa tattcaccca cacggagaat ttgatctcct
     9901 aaaacttttc cttggttatc tgtttcatct aatggtgtgg gatcaatcca gtttagtgga
     9961 gttgggtctc ctccggctaa ttctttatta acgatcaaat tattaacaag ctgatctaca
    10021 cgaaaaacgc cagggaaggt aacagtatca ccgaccttaa tggaaggttt tgtattgtca
    10081 ttttgaacca cagactgtgc gggctcttta atgggaatat agcgtcggtt tccagaaaaa
    10141 ctgaggtagc taagccatgt ataacctcca gcagtcacga ctttatcgta ataaacagat
    10201 tgacctgctt catagtaagc aagatcaggg ctatcgatac tggtttgtcc tttgactggt
    10261 aatctggttg taaagtgata agttccagag gggtcaaatg aagcttgaga aatgtgtttt
    10321 agttgtcttg ggtaggaatg acttgtctga gatgataagt ctttaaaatg gatataacca
    10381 cttacctgag attttggaat ttgacgctta tggtatcttt cagggccttg tccagcgttg
    10441 taattatact cttcgatagt gacagtgtct ccacggatat cagctaccca tgctacatga
    10501 ccgtaagcag catttgactg ataagcgttt ttatcaaacc aagcgatagc ccctatgctt
    10561 ggtgtcttat tcacaggata accctgattt ttcgcgatat gtccccacgt gcaggcatta
    10621 ccgtagcctt taggtaactg aaaaccatta gcagagctta aacgaaaagc tgcaaaagaa
    10681 gtgcattggc ggatatacat gttccacgaa tcgattccat tgcctttttt ccacttactc
    10741 ggatagttat cgcctaaaac agctgcatga acaagatttt cctgttgcga aataagtgta
    10801 gatggcatag taggtactag accattaaaa cctaaaagga ttactcctga gaccaaaaaa
    10861 cgatgaaatt ttttcattaa ttacctcctg taacttatta ttcgaaagca agaaacaaaa
    10921 aatgttattt ttagatgatt attttgaaaa ttggctttac ttgatattaa ataactgttt
    10981 gattggctaa tagagtgttc cttatttcaa gaattgtggt ctactatcac atttttacaa
    11041 tgtttcaatt attgataact aatacattat gtaaaaaagc gaacgtttat gatataatat
    11101 ctaattataa tacgaaagag gtggatgttt tgaaattact tgttgttggt tcaggtggtc
    11161 gtgaacatgc gattgctaag aagttgttag cgtctaaggg tgtggatcag gtttttgtgg
    11221 cacctggtaa tgatggtatg accttggatg gtctggactt ggtgaatata gtagtttccg
    11281 aacattccag gttgattgcc tttgctaagg agaatgagat ttcttgggcc tttattggtc
    11341 ctgatgatgc gctagcagct ggtatcgttg atgatttcaa cagtgctgga ctcagagctt
    11401 tcggtccaac aaaggcagca gcggagctag agtggtcaaa agactttgcc aaggaaatca
    11461 tggtcaaata caatgtccca acagcagcct atggcacatt ttcagacttt gaaaaagcca
    11521 aagcctacat cgaagagcag ggcgtaccta tcgtggtcaa ggctgatggc ttggcgctag
    11581 gtaagggtgt ggtcgtggct gaaaccgttg agcaggcggt agaggcggcg caagagatgc
    11641 ttctggacaa taagtttggc gattcaggag cgcgtgtggt tatcgaggaa ttcttggatg
    11701 gcgaggagtt ttccctcttt gcctttgtta atggtgataa gttctacatc atgccgacag
    11761 ctcaggatca caagcgtgcc tttgacggag acaaggggcc aaacactggt ggtatgggtg
    11821 cctatgcgcc agttcctcat ctacctcaga gcgtggtaga cacagcggtt gagacgatcg
    11881 ttaggcctgt ccttgagggc atggttgccg aagggcgtcc ttatctaggt gtcctctatg
    11941 tggggcttat cctgacagct gatggaccta aggttatcga gttcaactca cgtttcggtg
    12001 accctgaaac tcagattatc ctccctcgcc tgacttccga tttcgctcag aatattgacg
    12061 atatcatgat gggcattgag ccttacatca cttggcagaa ggacggtgtg actttgggtg
    12121 ttgtcgttgc ctcagaaggc tatccactcg attatgagaa aggtgtgcca ctgcctgaaa
    12181 agaccgacgg cgatatcatc acctactatg ccggagctaa gtttgtggaa aatagcgagc
    12241 tacttctctc aaacggagga cgtgtctata tgcttgtcac cacagaagac agcgtcaaag
    12301 cagggcagga caaaatctat acccagctag cccaacaaga cacaacaggt ctcttctacc
    12361 gaaatgacat cggaagcaaa gctataagag aataagacta accattcaaa tattagcgtc
    12421 agcaatcaac gaccgaaggt cgctccaaaa agaaagaaga aaggaactga acacgggact
    12481 aatttcctag gaaaaaaata aaactacctg gacacaagcg tcatcgttag ttttctgcgt
    12541 cgtaggcgtc tgatgctaaa gcacctagtc gccctgacgt gggttcgtct tcgaaatcaa
    12601 agatttcgga cttaccgcat atttttctac ggaaattttc gacaagtcga aacgcccctt
    12661 tgtatcttaa ttatgaaaac tccaattatt tctattatca tgggctccaa atctgactgg
    12721 gcaaccatgc aaaaaacggc tgaagttcta gacaactttg gcattgctta cgagaagaaa
    12781 gtcgtctctg cccaccgtac gccagacctc atgttcaagc atgctgagga agcacgtggt
    12841 cgtggcatca aaatcatcat cgcaggagca ggtggggcag ctcacttgcc tggtatggtg
    12901 gcagctaaaa caaccctgcc tgttatcggt gtgcctgtca aatcacgtgc cctttcaggt
    12961 ttggattcac tctattcgat tgtgcagatg cctggcggtg tgcctgtggc aaccatggct
    13021 atcggagaag cgggagcgac taacgctgcc ctgacagccc tccgtattct ctccatcgag
    13081 gatcaaaatc tggctgatgc gctagctcat ttccatgagg aacaaggaaa aatcgcagag
    13141 gagtcaagtg gtgaactcat ttaagaccat tgggattatc ggtggtggtc agctggggca
    13201 gatgatggcc attgcggcta tctatatggg ctacaaggtc atcacgctgg atcctgctag
    13261 cgactgccct gcctcccgcg ttagcgaggt aattgtggcg ccttacgatg atgttgaggc
    13321 tttgggacaa ttggctgcgc gttgcgatgt attgacttat gagtttgaga atattgatgc
    13381 ggacggtttg gatgccgttg tgtcagcttg tcagctaccg caggggaccg atctgctccg
    13441 catttcccaa aaccgtatct ttgaaaaaga ctttctggca aataaggctg gtgtgactgt
    13501 tgctccctat aaggtggtga cgtctagcct tgacctaggg ggacttgatt ttaccaagac
    13561 ctatgtcctc aagacagcga caggtggtta tgacggtcat gggcaaaagg ttatccgctc
    13621 agcagaagat ttgccagagg cgcagcaatt agctaactca gctcagtgtg tcttggaaga
    13681 gtttgtcaac tttgaccttg aaatctcagt catcgtgtct ggaaatggag aggatgtgac
    13741 ggtctttccc gttcaggaaa atatccactg caacaatatc ctgtcaaaaa ctatcgtgcc
    13801 agctcgtatc tcagatcaac tggctgataa ggctaagaaa atggcggtgc agattgccaa
    13861 gaaactccag ctgtcaggaa ctctctgtgt ggaaatgttt gcgaccgcag atgatatcat
    13921 tgtcaatgaa attgccccac gtccccacaa ctcagggcac tactcaatcg aggcttgcga
    13981 cttttcacag tttgacaccc acattttggg cgtgctagga gcgccgctcc caaccatcca
    14041 cctccatgct ccagccgtca tgctcaatgt cctcggacaa cacgtccagc aggcaattga
    14101 ctatgttgcc caaaacccta gcgcccacct ccacatgtat ggtaaactag aagcgaaaca
    14161 taaccgcaaa atgggacatg tgacggtgtt tagtgatttg cctgatgagg tggaggagtt
    14221 ttaggatgta tctaaagtat atatttaaaa taaaggatag gaatttagta tcggattatg
    14281 aatctttagt tcgaaatttg acaatcaatc ttcaaaaact agttattaaa gatagaactg
    14341 cattaatagg ggataaacaa cttccaatta cgatatatta tcgtggggag aatgaaaagt
    14401 ttcctacggg aatagtaaaa gttgaatcag actcagttga aggattagag ttatttgata
    14461 aaacgataaa ggaaatagtt gggaatgaaa gtagatttgg aaagttcaca tgtatatacg
    14521 atggaataag tacatattat tcaacagaaa tttattctga acttcatgaa attgagcgtc
    14581 gaacacaaga gtttttaagg gaagcatttt ttctgagcga taaaagtggg gaattgaaag
    14641 aatatcaggc aactatcaaa aaaatggata gtagtgattt ctcattcagc aaattaatta
    14701 atgaaatttt taaagcaagc gatataactg atacatttat tgaccatgtt agagcgtgtg
    14761 ttgaatttgg aacagttcca aatggagatt tattaccaaa aagtttttgg gatagtttag
    14821 atgactcttt actagatgat aaagagcaag gtttagagat taataattta ttaaatgaga
    14881 ttcgttcaac aagaaataca atagctcact gtaaagagtt taa
//
LOCUS       DATXIG010000037        14251 bp    DNA     linear   BCT 28-DEC-2023
DEFINITION  TPA_asm: Streptococcus pyogenes strain MGAS11113
            SAMN08555799-rid19682583.denovo.037, whole genome shotgun sequence.
ACCESSION   DATXIG010000037 DATXIG010000000
VERSION     DATXIG010000037.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN08555799
            Sequence Read Archive: SRR6740495
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pyogenes
  ORGANISM  Streptococcus pyogenes
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 14251)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14251)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-OCT-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-OCT-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001918058.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001918058.2
            Genome Coverage       :: 368x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/12/2023 11:23:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 1,806
            CDSs (total)                      :: 1,769
            Genes (coding)                    :: 1,722
            CDSs (with protein)               :: 1,722
            Genes (RNA)                       :: 37
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1, 1 (5S, 23S)
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 47
            CDSs (without protein)            :: 47
            Pseudo Genes (ambiguous residues) :: 0 of 47
            Pseudo Genes (frameshifted)       :: 30 of 47
            Pseudo Genes (incomplete)         :: 20 of 47
            Pseudo Genes (internal stop)      :: 9 of 47
            Pseudo Genes (multiple problems)  :: 11 of 47
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14251
                     /organism="Streptococcus pyogenes"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN08555799-rid19682583.denovo.037"
                     /strain="MGAS11113"
                     /serotype="Emm28"
                     /isolation_source="normally sterile site"
                     /host="Homo sapiens"
                     /db_xref="taxon:1314"
                     /country="Finland"
                     /collection_date="2002"
                     /collected_by="National Public Health Laboratory"
     gene            55..1251
                     /locus_tag="VC122_001204"
     CDS             55..1251
                     /locus_tag="VC122_001204"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001096748.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoglycerate kinase"
                     /protein_id="HEP5641128.1"
                     /translation="MAKLTVKDVDLKGKKVLVRVDFNVPLKDGVITNDNRITAALPTI
                     KYIIEQGGRAILFSHLGRVKEEADKEGKSLAPVAADLAAKLGQDVVFPGVTRGSKLEE
                     AINALEDGQVLLVENTRFEDVDGKKESKNDEELGKYWASLGDGIFVNDAFGTAHRAHA
                     SNVGISANVEKAVAGFLLENEIAYIQEAVETPERPFVAILGGSKVSDKIGVIENLLEK
                     ADKVLIGGGMTYTFYKAQGIEIGNSLVEEDKLDVAKDLLEKSNGKLILPVDSKEANAF
                     AGYTEVRDTEGEAVSEGFLGLDIGPKSIAKFDEALTGAKTVVWNGPMGVFENPDFQAG
                     TIGVMDAIVKQPGVKSIIGGGDSAAAAINLGRADKFSWISTGGGASMELLEGKVLPGL
                     AALTEK"
     gene            1514..2065
                     /locus_tag="VC122_001205"
     CDS             1514..2065
                     /locus_tag="VC122_001205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011527757.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aromatic acid exporter family protein"
                     /protein_id="HEP5641129.1"
                     /translation="MKTLRKLLSNYKFDIKKFKLGMRTLKTGLSVFLVLLVFHLFGWK
                     GLQIGALTAVFSLREDFDKSVHFGFSRIIGNSIGGLLSLVFFAFNEIFHQAFWVTLLI
                     VPICTMLCIMVNVACNNKSGIIGAVAALLIITLSIPTGQTFIYVTSRVFETFCGVFVA
                     ILVNTDVELIKNKWFNKKHSDVR"
     gene            2132..2503
                     /locus_tag="VC122_001206"
     CDS             2132..2503
                     /locus_tag="VC122_001206"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003058022.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MerR family transcriptional regulator"
                     /protein_id="HEP5641130.1"
                     /translation="MKEKELRRSMAVFPIGTVMTLTDLSARQIRYYEDQGLIKPERTQ
                     GNRRMFSLNDMDRLLEIKDFLSEGLNIAAIKREYVERQGKLMQKQKALTDADVRRILH
                     DEMLTQSGFSTPSQHIGNFRI"
     gene            2541..3887
                     /gene="glnA"
                     /locus_tag="VC122_001207"
     CDS             2541..3887
                     /gene="glnA"
                     /locus_tag="VC122_001207"
                     /EC_number="6.3.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009880482.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I glutamate--ammonia ligase"
                     /protein_id="HEP5641131.1"
                     /translation="MAITVADIRHEVKEKNVTFLRLMFTDIMGVMKNVEIPATKEQLD
                     KVLSNKVMFDGSSIEGFVRINESDMYLYPDLDTWIVFPWGDENGAVAGLICDIYTAEG
                     KPFAGDPRGNLKRALKHMNEIGYKSFNLGPEPEFFLFKMDDKGNPTLEVNDNGGYFDL
                     APIDLADNTRREIVNILTKMGFEVEASHHEVAVGQHEIDFKYADVLKACDNIQIFKLV
                     VKTIAREHGLYATFMAKPKFGIAGSGMHCNMSLFDNQGNNAFYDETDKRGMQLSEDAY
                     YFLGGLMKHAYNYTAITNPTVNSYKRLVPGYEAPVYVAWAGSNRSPLIRVPASRGMGT
                     RLELRSVDPTANPYLALAVLLEAGLDGIINKIEAPEPIEANIYTMTMEERNEAGIIDL
                     PSTLHNALKALQKDDVVQKALGYHIYTNFLEAKRIEWSSYATFISQWEIDHYIHNY"
     gene            complement(4115..5797)
                     /locus_tag="VC122_001208"
     CDS             complement(4115..5797)
                     /locus_tag="VC122_001208"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014635693.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease J"
                     /protein_id="HEP5641132.1"
                     /translation="MTNISLKPNEVGVFAIGGLGEIGKNTYGIEYQDEIIIVDAGIKF
                     PEDDLLGIDYVIPDYSYIVDNLDRVKALVITHGHEDHIGGIPFLLKQANIPIYAGPLA
                     LALIRGKLEEHGLWREATVYEINHNTELTFKNMSVTFFKTTHSIPEPVGIVIHTPQGK
                     IICTGDFKFDFTPVGDPADLQRMAALGEEGVLCLLSDSTNAEIPTFTNSEKVVGQSIL
                     KIIEGIHGRIIFASFASNIYRLQQAAEAAVKTGRKIAVFGRSMEKAIVNGIELGYIKV
                     PNGTFIEPSELKNLHASEVLIMCTGSQGESMAALARIANGTHRQVTLQPGDTVIFSSS
                     PIPGNTTSVNKLINTIQEAGVDVIHGKVNNIHTSGHGGQQEQKLMLSLIKPKYFMPVH
                     GEYRMQKVHAGLAMDIGIPKENIFIMENGDVLALTSDSARIAGHFNAQDIYVDGNGIG
                     DIGAAVLRDRRDLSEDGVVLAVATVDFNTQMILAGPDILSRGFIYMRESGDLIRESQR
                     VLFNAIRIALKNKDASIQSVNGAIVNALRPFLYEKTEREPIIIPMVLTPDKH"
     gene            complement(5799..6029)
                     /locus_tag="VC122_001209"
     CDS             complement(5799..6029)
                     /locus_tag="VC122_001209"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000639570.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-dependent RNA polymerase auxiliary subunit
                     epsilon family protein"
                     /protein_id="HEP5641133.1"
                     /translation="MIYKVFYQETKDQSPRRESTKALYLNIDATDELDGRIKARRLVE
                     DNTYYNVEFIELLSDKHLDYEKETGVFELTEF"
     gene            6313..7011
                     /gene="tsaB"
                     /locus_tag="VC122_001210"
     CDS             6313..7011
                     /gene="tsaB"
                     /locus_tag="VC122_001210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011054983.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (adenosine(37)-N6)-threonylcarbamoyltransferase complex
                     dimerization subunit type 1 TsaB"
                     /protein_id="HEP5641134.1"
                     /translation="MKTLAFDTSNKTLSLAILDDETLLADMTLNIQKNHSVSLMPAID
                     FLMTCTDLKPQDLERIVVAKGPGSYTGLRVAVATAKTLAYSLNIALVGISSLYALAAS
                     TCKQYPNTLVVPLIDARRQNAYVGYYRQGKSVMPQAHAPLEVIIEQLVEEGQLIFVGE
                     TAPFAEQIQKKLPQAILLPTLPSAYECGLLGQSLAPENVDAFVPQYLKRVEAEENWLK
                     DNEIKDDSHYVKRI"
     gene            6983..7438
                     /gene="rimI"
                     /locus_tag="VC122_001211"
     CDS             6983..7438
                     /gene="rimI"
                     /locus_tag="VC122_001211"
                     /EC_number="2.3.1.266"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011285150.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosomal protein S18-alanine
                     N-acetyltransferase"
                     /protein_id="HEP5641135.1"
                     /translation="MIVTTLSESNMKTVEEQAKNIYQLLEMVYGTSPWTLEQVLIDIR
                     RDQTDYFLLYDHDKLLGFLAIQDLAGEVEMTQIAILPSHQGLGLASQLMTHLDSIESD
                     IFLEVRESNHRAQGLYQKFGFKFIGKRPDYYRNPIETALLMKREGKHDR"
     gene            7428..8456
                     /gene="tsaD"
                     /locus_tag="VC122_001212"
     CDS             7428..8456
                     /gene="tsaD"
                     /locus_tag="VC122_001212"
                     /EC_number="2.3.1.234"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011285149.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (adenosine(37)-N6)-threonylcarbamoyltransferase complex
                     transferase subunit TsaD"
                     /protein_id="HEP5641136.1"
                     /translation="MTDRYILAVESSCDETSVAILKNESTLLSNVIASQVESHKRFGG
                     VVPEVASRHHVEVITTCFEDALQEAGISASDLSAVAVTYGPGLVGALLVGLAAAKAFA
                     WANHLPLIPVNHMAGHLMAAREQKPLVYPLIALLVSGGHTELVYVPEPGDYHIIGETR
                     DDAVGEAYDKVGRVMGLTYPAGREIDQLAHKGQDTYHFPRAMITEDHLEFSFSGLKSA
                     FINLHHNAKQKGNELILEDLCASFQAAVLDILLAKTKKALSRYPAKMLVVAGGVAANQ
                     GLRDRLAQEITHIEVVIPKLRLCGDNAGMIALAAAIEYDKQHFANMSLNAKPSLAFDQ
                     FPDSFVIN"
     gene            complement(8627..8896)
                     /gene="rpsN"
                     /locus_tag="VC122_001213"
     CDS             complement(8627..8896)
                     /gene="rpsN"
                     /locus_tag="VC122_001213"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012560987.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S14"
                     /protein_id="HEP5641137.1"
                     /translation="MAKKSKIAKYQKQLQLIEQYADLRRDLKAKGDYESLRKLPRDSN
                     PNRLKNRDKIDGRPHAYMRKFGVSRINFRDLAHKGQLPGVTKASW"
     gene            complement(9117..9875)
                     /locus_tag="VC122_001214"
     CDS             complement(9117..9875)
                     /locus_tag="VC122_001214"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002982924.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HEP5641138.1"
                     /translation="MSTNDLTKKLKKLKHVQVYNTIFQLIQDGTYSPGMQLPSEPELA
                     RQLNVSRMTLRKSLALLQEDHLIKNIRGKGNFILKTPETKYHQGFEYLQHPIYASLSS
                     DITKVELEYRIEVPTVAITASLKQETPVVIIVDRWYHSQNKAIAYSLSFIPIEVISKY
                     AINLNQEEPLLTFLEEKIYESGKASHSCNQIGYTKTGNYTATKYTLSENSAFILIQET
                     LYNGKDILVSTKHYVPADLFDLKVQSQSCQSDTH"
     gene            10115..10894
                     /gene="udp"
                     /locus_tag="VC122_001215"
     CDS             10115..10894
                     /gene="udp"
                     /locus_tag="VC122_001215"
                     /EC_number="2.4.2.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011184939.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="uridine phosphorylase"
                     /protein_id="HEP5641139.1"
                     /translation="MQNYSGEVGLQYHLQIRPGDVGRYVIMPGDPKRCAKIAEHFDNA
                     VLVADSREYVTYTGTLNGEKVSVTSTGIGGPSASIAMEELKLCGADTFIRVGTCGGID
                     LDVKGGDIVIATGAIRMEGTSKEYAPIEFPAVADLEVTNALVNAAKKLGYTSHAGVVQ
                     CKDAFYGQHEPERMPVSYELLNKWEAWKRLGTKASEMESAALFVAASHLGVRCGSDFL
                     VVGNQERNALGMDNPMAHDTEAAIQVAVEALRTLIENDKSQ"
     gene            10915..12117
                     /locus_tag="VC122_001216"
     CDS             10915..12117
                     /locus_tag="VC122_001216"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015058832.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NupC/NupG family nucleoside CNT transporter"
                     /protein_id="HEP5641140.1"
                     /translation="MQFIYSIIGILLVLGIVYAISFNRKSVSLSLIGKALIVQFIIAL
                     ILVRIPLGQQIVSVVSTGVTSVINCGQAGLNFVFGSLADSGAKTGFIFAIQTLGNIVF
                     LSALVSLLYYVGILGFVVKWIGKGVGKIMKSSEVESFVAVANMFLGQTDSPILVSKYL
                     GRMTDSEIMVVLVSGMGSMSVSILGGYIALGIPMEYLLIASTMVPIGSILIAKILLPQ
                     TEPVQKIDDIKMDNKGNNANVIDAIAEGASTGAQMAFSIGASLIAFVGLVSLINMMLS
                     GLGIRLEQIFSYVFAPFGFLMGFDHKNILLEGNLLGSKLILNEFVSFQQLGHLIKSLD
                     YRTALVATISLCGFANLSSLGICVSGIAVLCPEKRSTLARLVFRAMIGGIAVSMLSAF
                     IVGIVTLF"
     gene            12147..12818
                     /gene="deoC"
                     /locus_tag="VC122_001217"
     CDS             12147..12818
                     /gene="deoC"
                     /locus_tag="VC122_001217"
                     /EC_number="4.1.2.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011528957.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="deoxyribose-phosphate aldolase"
                     /protein_id="HEP5641141.1"
                     /translation="MEVKDILKTVDHTLLATTATWPEIQTILDDAMAYETASACIPAS
                     YVKKAAEYVSGKLAICTVIGFPNGYSTTAAKVFECQDAIQNGADEIDMVINLTDVKNG
                     DFDTVEEEIRQIKAACQDHILKVIVETCQLTKEELIELCGVVTRSGADFIKTSTGFST
                     AGATFEDVEVMAKYVGEGVKIKAAGGISSLEDAKTFIALGASRLGTSRIIKIVKNEAT
                     KTDSY"
     gene            complement(12913..14082)
                     /locus_tag="VC122_001218"
     CDS             complement(12913..14082)
                     /locus_tag="VC122_001218"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011106601.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HEP5641142.1"
                     /translation="MTQYDTIIIGGGPAGMMAAISSSYYGYKTLLIEKNRRLGKKLAG
                     TGGGRCNVTNSGNLDDLMAGIPGNGRFLYSVFSQFDNHDIIAFFEENGVKLKEEDHGR
                     MFPTTDKSRTIIDALEKKIKALGGQVLTSTEVVSVKKQDDLFYLKSTDQTFTCQKLIV
                     TTGGKSYPSTGSTGFGHDIARHFKLTVTDLEAAESPLLTDFPHKVLQGISLDDVTLSY
                     DKHVITHDLLFTHFGLSGPAALRLSSFVKGGEIAELDFLPHLSTDDLTAYLSDQRDKN
                     IKNALKGLLPERVADFLSEDYPEKVKQLSPKQEKELLDKLKHLQIPITGKMSLAKSFV
                     TKGGVDLKEINPKTLESKKVPGLYFAGEVLDINAHTGGFNITSALCSGWIAGKSS"
BASE COUNT         4344 a         2412 c         2978 g         4517 t
ORIGIN      
        1 ctaggaattg tggtataatg gaggagtata aaaaatatta aggagtctat tcacatggct
       61 aaattgactg ttaaagacgt tgatttgaaa ggtaaaaaag tcctcgttcg tgttgacttt
      121 aatgtgcctt tgaaagatgg cgttatcact aacgacaacc gtatcactgc ggctcttcca
      181 acaatcaagt atatcatcga acaaggtggt cgtgctatcc tcttctctca ccttggacgt
      241 gttaaagaag aagctgacaa agaaggaaaa tcacttgcac cggtagctgc tgatttagct
      301 gctaaacttg gtcaagatgt tgtattccca ggtgttactc gtggttcaaa attagaagaa
      361 gcaatcaatg ctttggaaga tggacaagtt cttttggttg aaaacactcg ttttgaagat
      421 gttgacggta agaaagaatc taagaatgac gaagaacttg gtaaatactg ggcttcactt
      481 ggagatggaa tcttcgttaa cgatgcattt ggtacagcac accgtgctca cgcatcaaac
      541 gtaggtattt cagcaaacgt tgaaaaagct gtagctggtt tccttcttga aaacgaaatt
      601 gcttacatcc aagaagcagt tgaaactcca gaacgtccat tcgtagctat tcttggtggc
      661 tcaaaagttt ctgataagat tggtgttatc gaaaatcttc ttgaaaaagc tgataaagtt
      721 cttatcggtg gtggtatgac ttacacattc tacaaagctc aaggtatcga aatcggtaac
      781 tcacttgtag aagaagacaa attggatgtt gctaaagacc tccttgaaaa atcaaatggt
      841 aaattgatct tgccagttga ctcgaaagaa gcaaacgcat ttgctggtta cactgaagtt
      901 cgcgacactg aaggtgaagc agtttcagaa ggcttccttg gtcttgacat cggtcctaaa
      961 tcaatcgcta aatttgatga agcacttact ggtgctaaaa cagttgtttg gaacggacct
     1021 atgggtgtct ttgaaaaccc tgacttccaa gctggtacaa tcggtgtcat ggacgctatc
     1081 gttaaacaac caggcgttaa atccatcatc ggtggtggtg attcagcagc agctgctatc
     1141 aaccttggcc gtgctgacaa attctcatgg atcagtaccg gcggaggcgc tagcatggaa
     1201 cttctcgagg gtaaagtatt accaggtttg gcagcattga ctgaaaaata atagtcatta
     1261 taatatatag taaagggcat gctgatgcat gtcttttttt agttgatcat gtacagttat
     1321 tgaactttcc ctaacttcac ttatcattta ctagttttta gatatagtgg tatttggctg
     1381 acactgcaat tttcaaaagt ttatgacgat ttcatatcaa ttggtttata aaagatatcc
     1441 taatcataaa taaaacgttt accaaaaggg ttttatggat tgatatgtta aaatagatag
     1501 tgaagggata ggtatgaaaa cgttaagaaa attattatct aattacaagt ttgatattaa
     1561 aaagtttaaa ttgggaatga gaacattgaa aacaggctta tcggtctttt tagttttatt
     1621 ggtttttcat ttatttggtt ggaaagggct tcaaattggt gctctaactg ctgtatttag
     1681 tttaagagaa gactttgata aaagcgtgca ctttggcttt tctagaatta ttggtaatag
     1741 tataggtggc ctactctcct tagtattttt tgcattcaat gagatatttc atcaagcttt
     1801 ttgggttact ttattaattg ttcctatttg taccatgtta tgtatcatgg ttaatgttgc
     1861 atgtaataat aaatcaggta ttattggggc agtggctgct cttttgatta ttacactgtc
     1921 tattccaaca ggacaaacgt ttatttatgt gacttcgcgg gtatttgaaa cattttgtgg
     1981 cgtctttgta gctattttag ttaatactga tgttgaactt attaaaaata aatggtttaa
     2041 caaaaaacac agtgatgtta gataatataa catatatact tgacaggtta tttttttcag
     2101 cctataataa ggaagatgaa aggagtttgt catgaaagaa aaagaactta gacgctcaat
     2161 ggctgttttt cctattggaa cggtgatgac attaacagat ctctctgcga gacaaattcg
     2221 ctattatgaa gatcaaggtt taattaagcc tgaacgaacg caagggaatc ggcgtatgtt
     2281 ttccttaaat gacatggacc gacttttgga aattaaagat tttttgtctg agggattaaa
     2341 tattgcagct attaaacgcg aatatgtcga gcgtcaaggt aagttaatgc aaaaacaaaa
     2401 agctctaaca gatgcagatg tcagacggat cttacatgat gaaatgctga cacaaagcgg
     2461 tttttctact ccctcacagc atattggtaa ttttcgtatt taagatatga attgtataaa
     2521 atttaaagga gacctattta atggcaatca cagtagctga cattcgtcat gaagtcaaag
     2581 aaaaaaatgt aacgtttctt cgcttgatgt tcactgatat catgggcgtt atgaaaaatg
     2641 tggagattcc tgcaacgaaa gaacagttag acaaagtatt gtctaacaag gttatgtttg
     2701 atggttcatc tatcgaaggt tttgtacgga tcaatgagtc agatatgtac ctttaccccg
     2761 atttagacac ttggattgtt tttccctggg gagatgaaaa tggagcagtt gcaggtttaa
     2821 tttgtgatat ttatacagca gaaggaaagc cttttgcagg agatcctaga ggaaatttaa
     2881 aaagagccct gaaacacatg aacgagatcg gctacaaatc atttaatctt gggccagaac
     2941 cagaattttt cctttttaag atggatgata aaggtaatcc gacacttgaa gttaacgata
     3001 atggtggtta ttttgattta gcgccaattg acttagcaga caacacgcgc cgtgaaattg
     3061 tgaatatttt aacgaaaatg ggttttgaag tggaagctag tcatcatgaa gtggctgttg
     3121 gtcaacatga gattgatttt aaatatgcag atgttttgaa agcttgtgat aatattcaaa
     3181 tttttaagct agttgtaaaa acgattgccc gtgaacatgg actttatgct acttttatgg
     3241 ctaaaccaaa atttggaata gctggatcag ggatgcactg taacatgtct ttgtttgata
     3301 accaaggtaa taatgctttt tatgatgaaa ctgataagcg aggaatgcag ctatcagaag
     3361 atgcttatta tttcttggga ggactaatga agcatgctta taactacact gctatcacta
     3421 accctacagt gaattcttac aaacgattag ttccgggtta tgaggcacct gtttatgtcg
     3481 cttgggctgg aagtaatcgt tcaccgctta tccgtgttcc agcatcacgt ggtatgggaa
     3541 cgcgtttgga gttacgttcg gttgatccga cagctaatcc ttatttagcc ttggctgttc
     3601 tcttggaagc tggattagat ggtatcatta acaaaattga agctccagaa cccattgaag
     3661 ctaacattta taccatgaca atggaagaac gaaatgaagc aggcattatt gatttgccat
     3721 caacgcttca taatgcctta aaagctcttc aaaaagatga tgtggtacaa aaggcactag
     3781 gttaccatat ctacactaat ttcttagaag caaaacgaat tgaatggtct tcctatgcaa
     3841 cttttatttc tcaatgggaa attgaccatt atattcataa ttattagact tatcaggacc
     3901 tgctcaagtt gagtgggtct tttggatgag ctattgtatt tgagctagca agctattatt
     3961 agctgatcgg acaccaattg attttccatt atagaaaact aaaaaccagt ccatgttcta
     4021 tgaggactgg tttttagttt gataattatc tgaactagaa ctgatatgtt aaagatatag
     4081 acagatagta ttaagaaatg agtcttattt gattttagtg cttatcaggt gttaagacca
     4141 tcggaattat aattggttca cgctcagtct tttcatacaa gaatggacga agagcattga
     4201 cgatagcacc gttaactgat tggatgctag catccttatt ttttaaagca atacggatag
     4261 cgttaaagag cacacgttgg ctttcgcgga tcaagtcacc tgactcacgc atataaatga
     4321 aaccacggct cagaatatct ggtccagcta aaatcatttg tgtgttaaaa tcaacagttg
     4381 caacagctaa gacaacacca tcttctgaca aatcacggcg gtctcgtaaa acggctgctc
     4441 caatatcccc gatgccatta ccatccacgt agatgtcttg ggcattaaag tgaccagcaa
     4501 tacgcgcact gtcactggtt aaagctagaa cgtctccatt ttccataata aagatatttt
     4561 cttttggaat accaatatcc atcgccagtc ctgcatgaac tttttgcatg cgatactcac
     4621 catgtactgg catgaaatat tttggtttaa tcaaactaag catgagcttt tgttcttgct
     4681 gtcctccatg accagaagta tgaatattat tgacttttcc atgaataaca tcaacaccag
     4741 cttcttggat ggtattgatt aatttattga cacttgtggt attaccaggg attggacttg
     4801 atgagaaaat cacagtatca ccaggctgta aagttacttg acgatgagtg ccattagcga
     4861 tacgagcaag tgctgccata gactcacctt gactacctgt acacataatt aaaacttcac
     4921 tagcatgaag atttttaagt tcgcttggct caatgaaggt accatttgga actttaatat
     4981 agccaagttc aataccgtta acaattgctt tttccataga acgaccaaaa acggcaattt
     5041 tacgtcccgt tttaacagca gcttctgcag cctgttgcaa acggtagatg tttgaagcga
     5101 aggaagcaaa aataatacga ccatgaatac cttcgataat ttttaaaatg gattggccaa
     5161 caactttttc agagttagta aaggttggga tttcagcatt agttgagtct gataataagc
     5221 aaaggacacc ttcttcacca agcgctgcca tacgttgtaa atctgcagga tctcctactg
     5281 gcgtgaagtc aaatttaaaa tcaccagtac agataatctt accttgaggg gtgtggataa
     5341 caataccgac cggttctgga atggaatgag tagtcttgaa gaaagtgacg ctcatgtttt
     5401 taaaagttaa ctcggtgttg tgattgattt cgtaaacagt tgcttcacgc caaagaccat
     5461 gttcttccaa tttaccacgg ataagagcaa gtgctaaagg tcctgcgtag atagggatat
     5521 tagcctgttt gagaagaaat ggaataccac caatgtggtc ttcgtgacca tgtgtaataa
     5581 caagagcttt tacccggtct aagttatcga caatgtaaga atagtctgga ataacgtaat
     5641 caattcccaa aagatcatct tctgggaatt tgataccggc atcaacgata atgatctcgt
     5701 cttgatactc aataccataa gtattcttcc caatctctcc cagaccacca atggcaaaca
     5761 ccccaacttc attaggtttt aaactgatat ttgtcatctt agaactccgt taattcaaaa
     5821 acaccggtct ctttttcgta atcgaggtgt ttgtcagaaa gtagctcgat aaattccacg
     5881 ttataatagg tgttatcttc aacgagacga cgagccttga tacggccatc gagttcatca
     5941 gtagcatcaa tattaagata gagtgcttta gtactttcac gacgtgggct ttgatccttt
     6001 gtttcttgat agaaaacttt ataaatcatt tgttttttat tcctttcgtt atgaatcaat
     6061 tatccaaaca ataataaata ggtcaaaagg taggtgttcc attttgacgg ttttatatgt
     6121 tgtcattatt gtgtagtaga ttaattcctg attctgtgca attattataa ttataccata
     6181 aacttagctt ttagtaaaaa atttaggtac atttatggta tagtctaaat caaattaaga
     6241 gtcagccttt ttgtttatga ttcttaattt gtgttataat tgatactaga acttgcagta
     6301 gaaaggcata aaatgaagac acttgcattt gatacctcaa ataaaacctt gtcccttgct
     6361 atacttgatg atgagacact tctagcagat atgaccctta acattcagaa aaatcatagt
     6421 gttagcctta tgcctgctat tgattttttg atgacttgta ctgatcttaa acctcaagat
     6481 ttagaaagaa tagtggttgc aaaaggccct ggatcttaca caggtttacg agtggcagtt
     6541 gctactgcaa aaacgttagc gtacagttta aatattgcat tggtcgggat ttccagtcta
     6601 tatgctttgg ctgcgtctac ttgtaaacag tatccaaata ctttggtggt gccattgatt
     6661 gatgctagaa ggcaaaatgc gtatgtaggt tattatcggc aaggaaaatc agtgatgcca
     6721 caagcccatg ctccactaga agttattata gaacaattag tagaagaagg gcagctgatt
     6781 tttgttgggg agactgctcc ttttgctgag caaattcaaa agaaactacc tcaggcgata
     6841 ctacttccaa ccctgccttc tgcttacgaa tgtggtcttt tggggcaaag tttggcacca
     6901 gaaaatgtag acgcctttgt ccctcaatat ctcaagagag tggaagctga agaaaactgg
     6961 ctcaaagata atgagataaa agatgatagt cactacgtta agcgaatcta atatgaaaac
     7021 agttgaagaa caagcaaaaa atatttatca gcttttggaa atggtttatg gcacgtctcc
     7081 ttggacttta gaacaagtat taatcgatat acggcgggat caaacagatt atttcctact
     7141 atatgatcat gataaattgc taggtttttt agctattcaa gatttagctg gtgaagttga
     7201 aatgactcaa attgctatac tacctagtca tcaaggactt gggctagcgt ctcaattgat
     7261 gacgcattta gatagcattg aaagtgacat ctttttagag gttagagaat ctaatcatag
     7321 agcacagggt ttatatcaga agtttggttt taaatttatt ggcaaaagac cagactacta
     7381 tagaaatcct atagaaacag ctttattgat gaaacgggaa ggaaaacatg acagataggt
     7441 atattttagc agttgagagt tcttgtgatg agaccagtgt agcgatttta aaaaacgaaa
     7501 gtaccctctt aagtaatgtc attgcaagtc aggttgagag ccacaaacgc tttggtggtg
     7561 tggtgccaga agtagctagt agacaccatg tcgaagtgat tacgacttgc tttgaggatg
     7621 ctttgcaaga agctggcatc agtgcttcag atttatcagc agttgcggta acttatggcc
     7681 ctggacttgt tggagccttg ttagttggat tagcagctgc gaaggctttt gcttgggcaa
     7741 atcatttacc tttgattccg gttaatcata tggcaggtca cttaatggca gcgcgtgaac
     7801 aaaagccttt agtttatcct ttaatagcac tattggtttc aggaggacac acagaattgg
     7861 tttatgtccc agagccaggg gattatcata ttattggcga gacgcgtgac gacgctgttg
     7921 gtgaggctta tgataaagtt gggcgtgtga tgggcttaac ctatccggca ggacgtgaaa
     7981 ttgatcagtt ggctcataaa ggtcaagata cttatcattt ccctcgtgct atgataacag
     8041 aagaccactt agaattttct ttttcaggac tcaagtccgc ttttatcaat cttcatcata
     8101 atgctaagca aaaaggcaat gaactgattc tagaagattt gtgtgcatct tttcaagcag
     8161 ctgttctaga tattttattg gccaaaacaa aaaaagctct gagcagatac cctgcaaaga
     8221 tgcttgtagt ggctggtggt gtagcggcaa atcaaggatt acgagataga ttagcacaag
     8281 aaattacaca tatagaagtt gtgattccta aactaagact ctgtggagat aacgcgggta
     8341 tgattgcttt agcagcagct attgagtatg acaaacagca ttttgctaat atgagcctaa
     8401 acgcgaagcc tagtttagct tttgaccagt ttccagacag ttttgtaata aactagtcac
     8461 acttgctagt tagtaagatt tataatggat aagaatctcc ctttgaaaaa acaacccggt
     8521 atcttggcat accgggttgt catgatgata agtaattggc ttctatggtt actaataatt
     8581 cagataatta cctgcaaaat aattgtctgc ctaaggtgaa gtaagcttac cagctagctt
     8641 tagtgacgcc aggaagctgt cctttgtgag ctaagtcacg gaaattgata cggctaacac
     8701 caaacttacg catgtaagca tgaggacggc cgtcaatttt gtcgcggttc ttcaagcggt
     8761 ttggatttga atcacgaggc aatttgcgca gtgattcata atcgcccttc gcttttaagt
     8821 cacgacgaag gtcagcgtac tgttcaatca gttggagctg cttttggtat ttagcaattt
     8881 ttgacttttt tgccattgct ttctccttct taatatttaa taacctcaga gtcgttatca
     8941 gcgctctgaa tccacttaac tggtatttaa ctggttaagt ggatatataa tcattataca
     9001 ataaataaaa gcaaaagcaa ataaaaaaaa ttaactagtt atctttttta gccataatat
     9061 caaaaaactc cacatctaag tgtgggagtc aggtattaat aactgtttaa aggtgtttaa
     9121 tgagtgtcgc tttggcaact ttgagattga acttttaagt caaataaatc agcaggaacg
     9181 tagtgtttgg ttgagaccaa gatgtcttta ccattgtaga gagtttcttg gattaaaata
     9241 aaagcactat tttctgatag agtatactta gttgctgtgt aattgccagt cttggtatag
     9301 ccgatttgat tgcaggaatg agaagcttta ccagattcat agattttctc ttctaagaaa
     9361 gtaagaaggg gctcttcttg attgagattt atagcatatt tagaaataac ttcaataggg
     9421 ataaaagata aactataagc aatagcttta ttttggctat gataccagcg atcaacaata
     9481 atcacaacag gagtttcttg ttttagggat gctgtaatgg caacagtggg cacttcaatc
     9541 cgatattcca attcaacctt tgtgatgtca gatgataggc ttgcatatat ggggtgttga
     9601 agatattcga aaccttggtg atatttagtt tcaggagtct tgagtataaa atttcccttt
     9661 cctctgatat tttttatgag atgatcctct tggaggaggg ctagggattt acgcagtgtc
     9721 atccgactga cattgagctg tctagcaagt tcaggttctg atggcagttg catgccagga
     9781 ctatatgtac catcttgaat aagttgaaaa atagtattat atacttgcac atgtttaagt
     9841 tttttgagtt ttttggttaa gtcgttagta gacatatcac ttacctcatt aataatcttt
     9901 agtggagttt aacttgtcta aaaactattt tagcactaac agtattcctt gattagaatt
     9961 gtacccatct atagtgttag tatctcacac tcaggtactt tttgtcaaaa aagagatgaa
    10021 aaacacctta aaaaagcgtt tacaaaatag agtgtgtgtg atactataca agtgtatcaa
    10081 aaattagaca agtaaaatac aggaggtttt taacatgcaa aattattcag gtgaagtcgg
    10141 attgcaatat cacctacaga ttcgtccagg tgatgtaggt cgttatgtta tcatgccagg
    10201 tgatccaaaa cgttgtgcaa aaattgcaga acattttgat aatgcagttc ttgtagcaga
    10261 cagccgtgaa tatgttacgt atacaggtac attaaacggt gaaaaagtta gtgttacttc
    10321 aactggtatt ggtggtccat cagcgtctat tgcgatggaa gaattgaaac tctgtggcgc
    10381 tgataccttt atccgtgttg gaacatgtgg tggtattgac cttgatgtta aaggcggaga
    10441 catcgttatt gcaactggag ctatccgtat ggaagggacc agtaaagaat atgctccgat
    10501 tgaatttcct gctgtagctg acttggaagt gacaaatgct ctagttaatg cagctaaaaa
    10561 gcttggttat acaagtcacg caggagtagt tcaatgtaaa gatgcatttt acgggcaaca
    10621 tgagccagaa cgtatgccag ttagttatga attattaaat aaatgggaag catggaaacg
    10681 tctgggaaca aaagcgtctg aaatggaatc tgcagctctt tttgtagcag caagtcatct
    10741 aggagttcgt tgtggatcag atttccttgt tgttggtaat caagaacgca acgctttggg
    10801 tatggataac ccaatggctc atgatacaga agcagctatt caagttgctg ttgaagcact
    10861 ccgcacactt attgaaaatg ataaatcaca ataagcgagg ttttggaagt catcatgcaa
    10921 tttatttata gtattattgg tattttattg gtattaggaa ttgtgtatgc aatttctttc
    10981 aatcgtaaga gtgtttctct aagtttaatt ggaaaagctc ttatcgttca attcattatt
    11041 gcgctaatct tagtacgtat cccactaggc caacaaattg ttagtgttgt ttcaactgga
    11101 gttactagcg taatcaactg tggtcaagct ggtttaaatt ttgtgtttgg gtcattagca
    11161 gatagtggcg caaaaactgg ttttattttc gctattcaaa cgcttggtaa tattgttttc
    11221 ttatctgccc tagttagtct actttattat gtaggaatcc ttggatttgt agtaaaatgg
    11281 ataggtaagg gcgttggtaa aattatgaaa tcctcagagg ttgagagttt tgttgccgta
    11341 gctaatatgt ttcttggtca aacagacagt ccaattttgg ttagcaaata cctaggtcgt
    11401 atgactgata gtgagataat ggttgtgttg gtatcaggta tgggaagtat gtcagtttct
    11461 attcttggtg gctatattgc attaggcatt ccaatggaat atctcttgat tgcttcaaca
    11521 atggttccta ttggcagtat tctcattgct aaaatcttat tgcctcaaac agaacctgtt
    11581 caaaaaattg atgacattaa gatggataat aaaggtaata acgccaatgt gattgatgca
    11641 atcgctgagg gtgcaagcac aggtgcacaa atggctttct caattggggc tagtttgatt
    11701 gcctttgttg gtttagtttc tttgattaat atgatgttaa gtggattggg aattcgctta
    11761 gaacaaatct tctcatatgt ttttgctcca tttggttttc ttatgggatt tgaccacaaa
    11821 aacattcttc tagaaggaaa ccttcttgga agtaagttga ttttaaatga gtttgtttcg
    11881 ttccaacaat tgggtcacct aatcaaatct ttagattatc gtacagcatt ggtagcaact
    11941 atttcactct gtggttttgc taatttatca agtttaggta tttgtgtttc aggtattgct
    12001 gttctttgcc cggagaaacg tagcacccta gctcgacttg ttttccgtgc aatgattggt
    12061 ggtattgctg taagtatgct tagcgccttt atcgtcggta ttgtaactct attctaaacg
    12121 ttgacaaaag aaagaaggat agtaacgtgg aagtaaaaga tattttaaaa acggtagacc
    12181 atactttgct agcaacaaca gcaacgtggc cagaaatcca aacaatttta gatgatgcca
    12241 tggcttatga aacagcttca gcatgtattc cagcttctta cgtcaaaaaa gcagcagaat
    12301 acgtttcagg taaattagct atttgtactg ttattgggtt cccaaatggc tatagtacaa
    12361 ctgcagcgaa ggtttttgaa tgtcaagatg ctattcaaaa tggtgctgat gaaattgaca
    12421 tggtcattaa tttgacagac gttaaaaatg gggattttga tactgttgaa gaagaaattc
    12481 gtcaaatcaa agccgcatgt caagaccata tcttaaaagt tatcgttgag acatgtcaat
    12541 taactaaaga agaacttatc gaactttgtg gagttgtcac acgttcaggt gcagacttta
    12601 ttaaaacctc tactggtttt tcgacagcag gtgctacatt tgaagatgtt gaagtgatgg
    12661 caaaatatgt cggcgaaggt gttaaaatta aggcagcagg tggaatctca tcattggaag
    12721 atgctaaaac atttattgct ttaggagctt cacgcttggg tactagccgt atcatcaaga
    12781 ttgttaagaa cgaagctaca aaaaccgata gctattaact taatcatacc gatttagctt
    12841 gagactaaaa caaaaaactc atataatagg agttaaaaag cctactatct tagtgataat
    12901 aggctttttt agctatgaag acttccctgc gatccatcct gaacaaagag ctgaggtaat
    12961 attaaaccct ccggtgtgag cattaatatc taagacctct ccagcgaaat acaggccagg
    13021 aactttcttg ctttccaagg ttttagggtt gatttctttt aaatctacgc ctcctttggt
    13081 cacaaatgat ttagccaaag acatttttcc tgtaatgggt atctggaggt gtttgagttt
    13141 atcaagcaat tccttttctt gttttggaga gagttgtttg accttttcgg ggtaatcttc
    13201 tgataaaaaa tcagctactc gttcaggcag taggccttta agagcatttt tgatgttttt
    13261 atcacgttga tcactcagat aagcggttaa gtcatctgtt gataagtgtg gcaaaaagtc
    13321 cagctctgca atttctccac ctttcacaaa agaggacaga cgaagagctg caggtcctga
    13381 aaggccaaag tgggtgaaga gtaagtcgtg tgtgatgacg tgtttgtcat aactaagagt
    13441 aacgtcgtct agggaaatac cttgcaagac tttgtgagga aaatcagtta acagagggct
    13501 ctcagcagct tctaaatcag tcacggtcag tttaaaatgc cgtgcaatat catgtccaaa
    13561 acctgttgaa cccgttgaag ggtaggactt gcctcctgtg gtcacaatca atttttggca
    13621 ggtgaaagtc tggtctgtag atttgaggta gaagaggtca tcttgttttt taacggaaac
    13681 cacttcagtg ctggttagca cctgaccccc aagagccttg atttttttct ccagagcatc
    13741 aatgattgtc cttgatttat ctgttgtggg gaacatccgt ccatggtctt cttctttgag
    13801 tttgacgccg ttttcctcga aaaaggcaat gatgtcatgg ttgtcaaact gggaaaaaac
    13861 actatataaa aagcggccat tcccaggaat gcctgccata aggtcatcaa ggttaccact
    13921 attagtgaca ttacagcgtc caccaccggt tcccgctaat tttttaccta agcggcgatt
    13981 tttttcaatt aagagagttt tatagccata gtagctgctg gagatggcgg ccatcatacc
    14041 agcagggcca cctccaataa taatcgtatc gtattgagtc atagccttat tttagcataa
    14101 aagcgggggg tttaatagtc aggtaaatga actaacctgg cataccgttt ttttacgtat
    14161 ctaaacaatg cctccatgac atcaaaaagc tagaaaaata gtccagatga ttaaaattat
    14221 ctttttatga aaacggttgc ttttttattt a
//
LOCUS       DATXIG010000038        14182 bp    DNA     linear   BCT 28-DEC-2023
DEFINITION  TPA_asm: Streptococcus pyogenes strain MGAS11113
            SAMN08555799-rid19682583.denovo.038, whole genome shotgun sequence.
ACCESSION   DATXIG010000038 DATXIG010000000
VERSION     DATXIG010000038.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN08555799
            Sequence Read Archive: SRR6740495
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pyogenes
  ORGANISM  Streptococcus pyogenes
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 14182)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14182)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-OCT-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-OCT-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001918058.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001918058.2
            Genome Coverage       :: 368x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/12/2023 11:23:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 1,806
            CDSs (total)                      :: 1,769
            Genes (coding)                    :: 1,722
            CDSs (with protein)               :: 1,722
            Genes (RNA)                       :: 37
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1, 1 (5S, 23S)
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 47
            CDSs (without protein)            :: 47
            Pseudo Genes (ambiguous residues) :: 0 of 47
            Pseudo Genes (frameshifted)       :: 30 of 47
            Pseudo Genes (incomplete)         :: 20 of 47
            Pseudo Genes (internal stop)      :: 9 of 47
            Pseudo Genes (multiple problems)  :: 11 of 47
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14182
                     /organism="Streptococcus pyogenes"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN08555799-rid19682583.denovo.038"
                     /strain="MGAS11113"
                     /serotype="Emm28"
                     /isolation_source="normally sterile site"
                     /host="Homo sapiens"
                     /db_xref="taxon:1314"
                     /country="Finland"
                     /collection_date="2002"
                     /collected_by="National Public Health Laboratory"
     gene            complement(<1..1865)
                     /locus_tag="VC122_001219"
     CDS             complement(<1..1865)
                     /locus_tag="VC122_001219"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002992712.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfatase-like hydrolase/transferase"
                     /protein_id="HEP5641143.1"
                     /translation="MIKDTFLKTNWLNISHHIILLVFGFYFSFYSLAKELVSSTAQPV
                     NYYAHLLNVSFVGYIISLIGLSYYLSRQVSRQLFLKTSFIVISYLIVSYWVQITQHLN
                     DKRFDIWSLTKNQFYQFQALPSLLIILVMATLIKILVAYFAIEKDRFGLLGYQGNTFS
                     VALILAVVPINDIHLLKLISSRFSELVRAGNSQIALLKISGLLIVLLVIFATIIYVVL
                     NALKHLKSNKPSFSVAATTSLFLALVFNYTFQYGVKGDEALLGYYVFPGATLFQIVAI
                     TLVALLAYVITNRYWPTTFFLLILGTIISVVNDLKESMRSEPLLVTDFVWLQELGLVT
                     SFVKKSVIVEMVVGLAICIVVAWYLHGRVLAGKLFMSPVKRASAVLGLFIVSCSMLIP
                     FSYEKEGKILSGLPIISALNNDNDINWLGFSTNARYKSLAYVWTRQVTKKIMEKPTNY
                     SQETIASIAQKYQKLAEDINKDRKNNIADQTVIYLLSESLSDPDRVSNVTVSHDVLPN
                     IKAIKNSTTAGLMQSDSYGGGTANMEFQTLTSLPFYNFSSSVSVLYSEVFPKMAKPHT
                     ISEFYQGKNRIAMHPASANNFNRKTVYSNLGFSKFLALSGSKDKFKNIENVGLLT"
     gene            complement(1862..3607)
                     /locus_tag="VC122_001220"
     CDS             complement(1862..3607)
                     /locus_tag="VC122_001220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012560595.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-L-Rha alpha-1,3-L-rhamnosyltransferase"
                     /protein_id="HEP5641144.1"
                     /translation="MNRILLYVHFNKYNKISAHVYYQLEQMRSLFSKIVFISNSKVSH
                     EDLKRLKNHCLIDEFLQRKNKGFDFSAWHDGLITMGFDKLEEFDSLTIMNDTCFGPIW
                     EMGPYFENFEEKEAVDFWGITNNRGTKAFKEHVQSYFMTFKNQVIQNKVFQQFWQSII
                     EYENVQEVIQHYETQLTSILLNEGFSYQTVFDTRKAESSFMPHPDFSYYNPTAILKHH
                     VPFIKVKAIDANQHIAPYLLNLIRETTNYPIDLIVSHMSQISLPDAKYLLSQKYLNCQ
                     RLAKQTCQKVAVHLHVFYVDLLDEFLTAFEDWNFHYDLFITTDSDIKRKEIKEILQRK
                     GKTADIRVTGNRGRDIYPMLLLKDKLSQYDYIGHFHTKKSKEADFWAGESWRKELIDM
                     LVKPADSILSVFETDDIGIIIADIPSFFRFNKIVNAWNEHLIAQEMMSLWRKMDVKKQ
                     IDFQAMDTFVMSYGTFVWFKYDALKSLFDLELTQNDIPSEPLPQNSILHAIERLFVYI
                     AWGNSYDFRIVKNPYELTPFIDNKLLNLREDEGAHTYVNFNQMGGIKGALKYIIVGPA
                     KAMKYIFLRLMEKLK"
     gene            complement(3604..4611)
                     /locus_tag="VC122_001221"
     CDS             complement(3604..4611)
                     /locus_tag="VC122_001221"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002990284.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HEP5641145.1"
                     /translation="MYKVSIICTNYNKAPWISDALDSFLSQVTDFEVEIIVIDDASTD
                     DSREILKSYQKKSSGKIKLLFNETNIGITKTWIKACLYAKGKYIARCDGDDYWTDSFK
                     LQKQVDVLEASKRSRWCNTDFDFVNSQGDLLYANAFSSGHTPLTDTYEKVLALKGMTM
                     ASTWLVDADLMRQVNQKINVDTPDDTFDIQLELFQLTQLTYIKDSTTVYRMTTDSDSR
                     PTDTPKMIHRIQKLLDTQLNYLKKYNQVDTKEMSELLLQQDAKQEIRIHELSCFIQEL
                     QQTIVDKTKQQETREVELQNVIEEQKNQLSELRQQYHAIINSRQWKYTSKLIAFIRRK
                     K"
     gene            complement(4636..5841)
                     /locus_tag="VC122_001222"
     CDS             complement(4636..5841)
                     /locus_tag="VC122_001222"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011184373.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEP5641146.1"
                     /translation="MINREIAVKVQHVSKTFKLPTEATKSFRTTLVNRLRGIKGFTEQ
                     HVLKDINFDVYKGDFFGIVGRNGSGKSTLLKIISQIYVPEKGQVTVDGKMVSFIELGV
                     GFNPELTGRENVYMNGAMLGFTKDEVDDMYNDIVDFAELHHFMNQKLKNYSSGMQVRL
                     AFSVAIKAQGDVLILDEVLAVGDEAFQRKCNDYFMERKDSGKTTILVTHDMGAVKKYC
                     NRAVLIEDGLVKAYGEPFDVANQYSVDNTETAEDAMNAEKISVSDIAKDLKVSLISNP
                     RITPNDTITFEVSYEVLKDDFTYIAFSLTDIDRNIWVYNDNSLDYPTQGIGRKCISYQ
                     CQLSQLNDIKLKLEVTVRDKNGQMLLFSTAEQSPQIVIQRNDIDQDDLSALDSASGLY
                     QRNGKWVFH"
     gene            complement(5841..6644)
                     /locus_tag="VC122_001223"
     CDS             complement(5841..6644)
                     /locus_tag="VC122_001223"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011528474.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEP5641147.1"
                     /translation="MNFLTKKNRILLREMVKTDFKLRYQGSAIGYLWSILKPLMMFTI
                     MYLVFIRFLRLGGNVPHFPVALLLANVIWSFFSEATSMGMVSIVSRGDLLRKLNFSKH
                     IIVFSAVLGALINFLINLVVVLIFALINGVTISGYAYLSLFLFIELVVLVLGIALLLS
                     NVFVYYRDLAQVWEVLLQAGMYATPIIYPITFVLDSHPLAAKLLMLNPVAQMIQDFRY
                     LLIDRANVTIWQMSTNWFYVVIPYLVPFVILFIGIFVFKKNADRFAEII"
     gene            complement(6647..7579)
                     /locus_tag="VC122_001224"
     CDS             complement(6647..7579)
                     /locus_tag="VC122_001224"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002985180.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HEP5641148.1"
                     /translation="MNINILLSTYNGERFLAEQIQSIQRQTVNDWTLLIRDDGSTDGT
                     QDIIRTFVKEDKRIQWINEGQTENLGVIKNFYTLLKHQKADVYFFSDQDDIWLDNKLE
                     VTLLEAQKHEMTAPLLVYTDLKVVTQHLAICHDSMIKTQSGHANTSLLQELTENTVTG
                     GTMMITHALAEEWTTCDGLLMHDWYLALLASATGKLVYLDIPTELYRQHDANVLGART
                     WSKRMKNWLTPHHLVNKYWWLITSSQKQAQLLLDLPLKPNDHELVTAYVSLLDMPFTK
                     RLATLKRYGFRKNRIFHTFIFRSLVVTLFGYRRK"
     gene            complement(7569..8723)
                     /locus_tag="VC122_001225"
     CDS             complement(7569..8723)
                     /locus_tag="VC122_001225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002985181.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1972 domain-containing protein"
                     /protein_id="HEP5641149.1"
                     /translation="MQDVFIIGSRGLPAKYGGFETFVEELISHQSSKNIRYHVACLSD
                     TKHKVHFDYKGADCFYLNPPKLGPARVIAYDMMAITYALSYSDQHQIQNPIFYVLGNT
                     VGAFIAPFVKQIHNRGGRFFINPDGLEWKRSKWSKPVQAYLKFSEKQMTRQADLVISD
                     NIGIDRYLKQVYPWSKTCFIAYGTQTQPSRLTTADSKVRAYFQTFDIREKDYYLILGR
                     FVPENNYETAIKEFMASSTKRDLVIICNHEDNAYFKQLLAETECDKDPRIKFVGTLYD
                     KELLAYIREQAYAYIHGHEVGGTNPGLLEALAHTNLNLVLGVDFNQSVAKSAALYWSK
                     QKGQLAELINQVDAGFDSDRLGKEAKAIIQEHYTWEKIVGEYEALFLNEH"
     gene            complement(8842..9696)
                     /gene="rfbD"
                     /locus_tag="VC122_001226"
     CDS             complement(8842..9696)
                     /gene="rfbD"
                     /locus_tag="VC122_001226"
                     /EC_number="1.1.1.133"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014407474.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dTDP-4-dehydrorhamnose reductase"
                     /protein_id="HEP5641150.1"
                     /translation="MILITGSNGQLGTELRYLLDERGVDYVAVDVAEMDITNEDKVEA
                     VFAQVKPTLVYHCAAYTAVDAAEDEGKALNEAINVTGSENIAKACGKYGATLVYISTD
                     YVFDGNKPVGQEWVETDRPDPKTEYGRTKRLGELAVERYAEHFYIIRTAWVFGNYGKN
                     FVFAMEQLAENHSRLTVVNDQHGRPTWTRTLAEFMCYLTENQKAFGYYHLSNDAKEDT
                     TWYDFAKEILKDKAVEVVPVDSSAFPAKAKRPLNSTMNLDKAKATGFVIPTWQEALKA
                     FYQQGLKK"
     gene            complement(9834..10172)
                     /locus_tag="VC122_001227"
     CDS             complement(9834..10172)
                     /locus_tag="VC122_001227"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002985185.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal-sulfur cluster assembly factor"
                     /protein_id="HEP5641151.1"
                     /translation="MSDTPKYTQDQVIAIKNRILEALETVIDPELGIDVVNLGLIYEI
                     RFNDNGYTEIDMTLTTMGCPLADLLTDYIHDALQDVPEVTKTEVKLVWYPAWTVDKMS
                     RYARIALGIR"
     gene            complement(10408..11517)
                     /gene="rpoD"
                     /locus_tag="VC122_001228"
     CDS             complement(10408..11517)
                     /gene="rpoD"
                     /locus_tag="VC122_001228"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012515467.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor RpoD"
                     /protein_id="HEP5641152.1"
                     /translation="MTKQKEITTFNVQVAEFIRHHKKEGTAIDDDVTEKLVIPFALDA
                     DQIDDLLERLTDGGISITDKEGNPSSKYIVEEPKPEELTDEELIGSNSAKVNDPVRMY
                     LKEIGVVPLLTSEEEKELAVAVAKGDLMAKQRLAEANLRLVVSIAKRYVGRGMQFLDL
                     IQEGNMGLMKAVDKFDYSKGFKFSTYATWWIRQAITRAIADQARTIRIPVHMVETINK
                     LVREQRNLLQELGQDPTPEQIAERMEMTPDKVREILKIAQEPVSLETPIGEEDDSHLG
                     DFIEDEVIENPVDYTTRVVLREQLDEVLDTLTDREENVLRLRFGLDDGKMRTLEDVGK
                     VFNVTRERIRQIEAKALRKLRHPSRSKQLRDFIED"
     gene            complement(11526..13340)
                     /gene="dnaG"
                     /locus_tag="VC122_001229"
     CDS             complement(11526..13340)
                     /gene="dnaG"
                     /locus_tag="VC122_001229"
                     /EC_number="2.7.7.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012560591.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA primase"
                     /protein_id="HEP5641153.1"
                     /translation="MGFLWGGDDLAIDKEMISQVKNSVNIVDVIGEVVKLSRSGRHYL
                     GLCPFHKEKTPSFNVVEDRQFFHCFGCGKSGDVFKFIEEYRQVPFLESVQIIADKTGM
                     SLNIPPSQAVLASQHKHPNHALMTLHEDAAKFYHAVLMTTTIGQEARKYLYQRGLDDQ
                     LIEHFNIGLAPDESDYLYQALSKKYEEGQLVASGLFNLSDQSNTIYDAFRNRIMFPLS
                     DDRGHIIAFSGRIWTAADMEKRQAKYKNSRGTVLFNKSYELYHLDKARPVIAKTHEVF
                     LMEGFMDVIAAYRSGYENAVASMGTALTQEHVNHLKQVTKKVVLIYDGDDAGQHAIAK
                     SLELLKDFVVEIVRIPNKMDPDEFVQRHSPEAFADLLKQSRISSVEFFIDYLKPTNVD
                     NLQSQIVYVEKMAPLIAQSPSITAQHSYINKIADLLPNFDYFQVEQSVNALRIQDRQK
                     RQGQIAQAVSNLVTLPIPKSLTAIAKTESHLMHRLLHHDYLLNEFRHRDDFYFDTSTL
                     EILYQRLKQQGYITSYDLSEMSEEVNRAYYNVLEENLPKEVALGEIDDILSKRAKLLA
                     ERDLHKQGKKVRESSNKGDHQAALEVLEHFIAQKRKME"
     gene            13470..13832
                     /gene="mscL"
                     /locus_tag="VC122_001230"
     CDS             13470..13832
                     /gene="mscL"
                     /locus_tag="VC122_001230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014635389.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="large conductance mechanosensitive channel
                     protein MscL"
                     /protein_id="HEP5641154.1"
                     /translation="MVKELKAFLFRGNIIELAVAVIIGGAFGAIVTSFVNDIITPLIL
                     NPALKAANVENITQLSWNGVKYGSFLGAVINFLIIGTSLFFVVKAAEKAMPKKEKEAA
                     APTQEELLTEIRDLLAQK"
     gene            complement(13960..14136)
                     /locus_tag="VC122_001231"
     CDS             complement(13960..14136)
                     /locus_tag="VC122_001231"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000048061.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S21"
                     /protein_id="HEP5641155.1"
                     /translation="MSKTVVRKNESLDDALRRFKRSVTKAGTLQESRKREFYEKPSVK
                     RKRKSEAARKRKKF"
BASE COUNT         4431 a         2738 c         2324 g         4689 t
ORIGIN      
        1 ctagtcaata aaccgacatt ttcaatgttc ttaaacttat ccttagaacc cgatagagct
       61 aagaatttgg aaaaacctaa attactataa actgtttttc tattaaaatt gttagcacta
      121 gcaggatgca tcgcaatacg attttttcct tggtaaaact cactaatcgt atgaggtttg
      181 gccattttag gaaagacttc tgaataaaga acagatactg aagaagaaaa attataaaaa
      241 ggtaagcttg ttaacgtttg aaactccatg ttagcagttc cacccccgta ggagtctgac
      301 tgcatgagtc ccgcagttgt gctatttttg attgccttga tattaggtaa aacatcgtgg
      361 ctaacagtaa catttgatac tctatcagga tctgacaaac tttcacttaa aagataaata
      421 accgtttggt cagcaatatt attttttctg tctttattaa tatcttctgc taatttttgg
      481 tacttctgag cgatactcgc tattgtttct tggctataat ttgtcggttt ttccattatt
      541 ttcttggtca cctgtcttgt ccaaacatat gctaaagatt tgtacctagc atttgttgaa
      601 aaacctaacc agtttatatc attatcatta tttaaagccg aaataatcgg aagaccagat
      661 aatattttac cttctttttc ataagaaaat ggtattaaca tactacaaga tacaataaat
      721 aaacctaata cagcacttgc ccgtttgaca gggctcataa ataatttccc tgctaaaact
      781 cggccatgta gataccaagc taccacaata caaatagcaa gtcctacaac catttctaca
      841 atcaccgatt ttttaacaaa gcttgtcact aaacctaatt cttgtaacca aacaaaatca
      901 gttactaata acggctcgct tctcattgat tcttttaaat cattaacaac agaaataatt
      961 gttcccagaa taagcaaaaa gaaggtagtt ggccaatatc tattcgttat cacgtatgct
     1021 aaaagagcaa ctagtgtaat agctactatc tgaaaaagag tagctccagg gaaaacataa
     1081 tatcctagca atgcttcatc accttttacg ccatactgaa acgtatagtt aaaaactaat
     1141 gctaaaaaca aactagtagt agctgctact gaaaatgaag gtttatttga cttaaggtgt
     1201 tttagagcat ttaaaaccac gtatatgatt gttgcaaata tgacaagtaa aactatcaac
     1261 agtccactta tttttaacag tgcaatttgg ctattacctg ctcttactaa ttcagaaaat
     1321 cgagaactta ttagttttaa cagatgtatg tcattaattg gcacaactgc taaaatcaga
     1381 gctacagaaa aagtattacc ttgatagcct aatagcccaa atctatcttt ttctattgca
     1441 aaatatgcta ccaatatttt tattaaagtg gccatcacta aaataatgag taaagaaggt
     1501 agagcttgaa attgataaaa ttgattttta gttaatgacc agatatcaaa ccgtttatca
     1561 ttcaggtgct gtgttatttg tacccaatag ctgacaatta gataagatat cacaataaaa
     1621 ctagttttca aaaacaactg tcgactaact tggcgactca aataataaga taatccaatc
     1681 agtgatataa tatatcccac aaaagaaaca tttagtaaat gagcataata gtttaccggt
     1741 tgtgccgtgg agcttactag ttctttcgcc aaactgtaaa aactgaaata aaaaccgaaa
     1801 acaagaagga taatatggtg actaatattt aaccaattgg tttttaaaaa tgtgtcttta
     1861 atcatttaag tttctccatt aaacgcaaga atatatactt cattgcttta gcgggaccaa
     1921 cgataatata ttttaatgct cctttgatac cgcccatttg attaaagtta acgtaggtat
     1981 gagctccttc atcttcacgt aaatttaaca gtttattgtc aataaaaggt gttaactcat
     2041 agggattttt aacaatccta aaatcataag aattccccca cgctatatag acaaataacc
     2101 gctcaatcgc atgtaatata gagttttgtg gtaaaggttc acttggaata tcattttgag
     2161 ttaattctaa atcaaaaagt gattttagag catcgtattt aaaccataca aatgttccat
     2221 aactcataac aaatgtatcc atagcttgaa aatcaatttg ttttttaaca tccatttttc
     2281 tccacaaaga catcatttct tgagcgatta aatgctcatt ccaagcattg acaatcttat
     2341 taaatctaaa aaaacttgga atatctgcga ttataatacc aatatcatct gtctcaaaaa
     2401 cgctaagaat actatctgct ggtttcacga gcatatcaat caattctttt cgccaagatt
     2461 ctcctgccca aaaatcagct tcttttgatt ttttcgtatg gaaatgacca atataatcat
     2521 attgagaaag tttatctttt agtaataaca ttggataaat atctcttcct ctatttcccg
     2581 taacgcgtat atcagcagtt ttaccttttc tttgaagtat ttctttgatc tcttttcttt
     2641 tgatatcact atctgttgta ataaaaagat cataatggaa gttccagtct tcgaaagctg
     2701 ttaaaaattc atctagcaaa tcaacgtaaa aaacatgtaa atgaacagct accttttgac
     2761 aagtttgttt tgctaaccgt tggcagttta ggtatttttg agataaaagg tacttagcat
     2821 ctggcaaaga gatttgcgac atatgtgaaa caataagatc tataggataa ttggttgttt
     2881 ctcgtatcag attcagtaag taaggagcga tatgctggtt agcatcaata gcttttactt
     2941 ttataaaagg cacatggtgt tttagaattg ctgttggatt ataatatgaa aaatccggat
     3001 gtggcatgaa tgacgactca gcttttctag tatcaaagac cgtctgataa ctaaagcctt
     3061 catttaagag aatggatgtt aattgcgttt cataatgctg aatcacttct tgaacgtttt
     3121 catattctat tatcgattgc caaaattgtt gaaaaacttt gttttgaatg acttgatttt
     3181 taaacgtcat aaaataactt tggacatgtt ctttaaaagc ttttgtacct ctattattag
     3241 taattcccca aaagtcaact gcctcttttt cttcaaaatt ttcaaaataa ggacccatct
     3301 cccaaatagg accaaaacaa gtatcgttca taatcgtcaa tgagtcaaac tcttccaact
     3361 tgtcaaatcc catagtaatc aagccatcgt gccatgcaga aaaatcaaaa cctttatttt
     3421 tgcgttgcaa aaactcatca ataagacagt ggtttttgag acgttttaaa tcttcatgac
     3481 tcactttact atttgagata aaaacaatct tagaaaacag tgatctcatt tgttccaatt
     3541 gatagtaaac atgtgcactt attttattgt atttgttgaa gtgaacatat aataaaatac
     3601 gattcatttt tttctcctaa taaaagcaat gagcttagac gtgtatttcc attgacgtga
     3661 attaataatg gcatggtatt gttgtcttaa ttcagatagt tgattttttt gttcttctat
     3721 gacattttgc aactccactt cacgtgtttc ttgctgcttt gttttatcta cgatagtttg
     3781 ttgtaattct tgaataaagc aactcaactc atgaattcgt atttcttgct tagcatcttg
     3841 ttgtaataat agttcagaca tttcttttgt atctacctga ttatactttt ttaagtaatt
     3901 taactgagta tcaagtagtt tttgaatacg atgaatcatt tttggtgtat cagtaggtct
     3961 tgaatcagaa tcggttgtca tgcgataaac cgtcgttgag tctttaatat aggtcagttg
     4021 agtcagttga aataactcta gttgaatgtc aaaagtatcg tctggtgtat ctacattgat
     4081 tttttggttt acttgtctca ttaaatcagc atcaactaac catgtcgatg ccatcgtcat
     4141 cccttttaag gctaaaactt tttcgtaagt atctgtcaaa ggtgtatgac cagatgaaaa
     4201 agcattagca tataataagt cgccttgact atttacaaag tcaaaatcag tattacacca
     4261 tcgtgaccgt ttggaagctt ctaagacatc aacttgtttt tggagtttaa aactatccgt
     4321 ccagtaatca tcaccatcac atctagcaat atatttgcct tttgcataaa ggcatgcttt
     4381 tatccaagtc tttgttattc caatattagt ttcgttaaag agtaatttaa tttttcctga
     4441 agactttttt tgataactct ttaaaatctc tcgtgagtca tctgtagaag catcatcaat
     4501 aacaataatt tcaacctcaa aatctgtcac ttggcttaaa aaactgtcaa gtgcatcaga
     4561 tatccaagga gctttgttat aattggtgca aataattgaa actttataca taaattccct
     4621 tctagctatt actcattaat gaaaaaccca cttcccatta cgttgataca agccacttgc
     4681 tgaatctaga gctgacagat catcttgatc aatatcgttt ctttgaatca ctatctgggg
     4741 tgattgttct gctgttgaaa agagtagcat ctgaccgttc ttatctcgga cagttacttc
     4801 taattttagt tttatatcat taagttgaga tagttgacat tggtaggaaa tgcattttct
     4861 acctatacct tgtgttggat aatctaaaga attgtcgttg taaacccaaa tgtttctatc
     4921 aatatccgtt aaagaaaagg caatgtaggt aaaatcatct tttaaaactt cataagaaac
     4981 ttcaaatgtg attgtgtcat ttggtgttat tcttggattt gagattaatg acactttcaa
     5041 atctttggca atatcagata cagatatttt ttcagcattc attgcatcct cagctgtctc
     5101 tgtattatct acactatact gattagcaac atcaaatggt tctccatatg ctttaacaag
     5161 accatcttca ataagaactg cacggttaca atacttctta acagctccca tatcatgtgt
     5221 cactaaaata gtcgtcttac cactgtcttt tctttccata aaataatcat tacattttcg
     5281 ctgaaaagct tcatctccta cagcaagcac ctcatctaaa ataagaacgt caccttgggc
     5341 tttaatagcc actgaaaatg ctaaacgtac ctgcatgcca gaagaataat tttttaattt
     5401 ttgattcata aaatgatgca actctgcaaa atcaacaata tcattgtaca tatcatcaac
     5461 ttcgtctttg gtaaatccca gcatggcgcc gttcatatag acgttttctc gtcctgttaa
     5521 ttctggattg aatcccactc caagctctat aaaagaaacc atcttcccat ctactgtaac
     5581 ctgtcctttt tctggaacat agatctggga aataatttta aggagcgttg attttcccga
     5641 gccgttacgc ccaacaattc caaaaaaatc gcctttataa acatcaaagt taatatcttt
     5701 tagcacatgt tgttcagtaa atcctttaat ccctcttagg cgattaacca gagtcgtgcg
     5761 aaaactttta gttgcttctg taggcagttt aaatgtttta ctaacatgct gtacttttac
     5821 tgcaatttct ctattaatca ttaaataatc tccgcaaatc tatcggcatt tttcttaaag
     5881 acaaagatgc caataaataa tataacaaat ggtactaaat atggaataac aacgtaaaac
     5941 caattggttg acatctgcca aatcgttacg ttggctctgt caatcaataa ataacgaaaa
     6001 tcttgaatca tttgtgctac tggatttagc atcaacaact ttgccgccaa agggtggcta
     6061 tctaaaacaa aagtgatcgg ataaatgatt ggagtggcat acatacctgc ttgtaatagt
     6121 acttcccaga cttgagctaa gtcacgataa taaacaaaga cattcgacaa taacaatgca
     6181 attccaagca ctaaaacaac taattctata aaaagaaaaa gagagagata agcataccct
     6241 gatatagtca caccatttat caacgcaaaa attaaaacaa caaccaaatt aataagaaaa
     6301 ttaattaaag ctcctaacac tgccgaaaaa acaatgatgt gcttagaaaa gttcaatttt
     6361 cgcaacaagt ctccccgaga tacaatagat accattccca tgctagttgc ttctgaaaaa
     6421 aaagaccaga tgacatttgc caataaaagc gctactggaa aatgaggtac atttccaccc
     6481 aggcgaagaa aacgaataaa taccaagtac ataatcgtaa acatcataag cggttttaag
     6541 atagaccata gatagccaat tgcgctccct tgatagcgta atttaaaatc cgtttttacc
     6601 atttctctca gtaaaatacg gtttttcttt gttaaaaaat tcattcttac tttctcctgt
     6661 aaccaaataa ggtaacaact aaactacgaa aaataaaggt gtgaaagatg cgattttttc
     6721 taaaaccgta ccttttcaag gtagctaagc gtttggtaaa aggcatatcc aataaagaaa
     6781 cataggccgt taccaattca tggtcatttg gttttaatgg taaatctaat agtaattgag
     6841 cctgtttttg gcttgaagta atcaaccacc aatatttgtt gactaaatga tggggggtta
     6901 accaattttt catgcgttta gaccaagttc tagctcccaa cacgttagca tcgtgctgac
     6961 ggtataattc tgttggaata tcaaggtaaa ctaatttccc agtagcagaa gctagtaaag
     7021 ctaaatacca gtcatgcatc aataaaccgt cacaagtggt ccattcctca gccagagcat
     7081 gagtaatcat cattgtgccg cctgttacgg tattttcggt taattcttga agtaggcttg
     7141 tgtttgcatg accagattgg gtcttaatca tactgtcatg gcaaatagct aaatgttggg
     7201 ttaccacttt gaggtctgta taaaccaata aaggagctgt catttcatgt ttttgagcct
     7261 ccaataaggt cacttccagt ttattgtcta accatatatc atcttggtca ctgaaaaaat
     7321 agacatctgc tttttggtgc tttaagaggg tgtaaaaatt cttaataact cccagatttt
     7381 ctgtctgccc ctcattgatc cactgaattc gtttgtcttc cttgacaaat gtgcgaataa
     7441 tatcctgggt gccatcagtt gatccatcat ctctaatcaa taaggtccag tcgttaactg
     7501 tttgcctttg gatactttga atttgttctg ctaagaagcg ttctccattg taggtggata
     7561 gtaaaatatt aatgttcatt taaaaataaa gcctcgtact cccctacaat tttttcccaa
     7621 gtgtaatgtt cttgaataat agctttggct tctttgccca gacgatcact atcaaaacct
     7681 gcatcaactt ggttaatcag ctctgctaat tgcccttttt gcttggacca atacagcgca
     7741 gcagacttag ccaccgactg gttaaaatca actcctaaaa ccaaattaag attggtatgg
     7801 gctagtgctt ctagaaggcc tggattagtc cctcctacct catgaccatg gatataggca
     7861 taagcctgtt ctcgaatgta ggctaatagt tctttgtcat aaagggtacc aacaaattta
     7921 atgcgtgggt ctttatcaca ctctgtctca gcaagtaatt gcttaaagta agcattgtct
     7981 tcatgattac aaataatcac taaatcacgc ttagttgagg aagccataaa ttctttaata
     8041 gcagtttcgt aattattttc aggaacaaag cgtcctaaaa tgagatagta atccttttct
     8101 ctaatatcaa aggtttgaaa ataagctctg accttgctat ctgctgttgt caaacgtgaa
     8161 ggttgtgtct gcgttccata agcaataaaa catgttttag accaaggata aacctgttta
     8221 aggtagcggt caatgccaat attatctgaa atgaccaaat cagcctgtct agtcatttgc
     8281 ttttcggaaa acttcaggta ggcctgcact ggttttgacc atttagaccg tttccactct
     8341 aacccgtctg gattgataaa aaagcgtcca cctcgattgt gaatctgctt cacaaatggc
     8401 gcaataaaag cgcctacagt attgcccaac acataaaaaa tagggttctg aatctggtgt
     8461 tggtcgctat aagacagggc ataggtaatt gccatcatat cataagcaat aactctagca
     8521 ggaccaagtt ttgggggatt aaggtaaaag caatctgccc ccttataatc aaaatgaacc
     8581 ttatgcttcg tatcgcttaa acaagcaacg tgataacgaa tgtttttaga tgattgatga
     8641 ctaatcaact cttcgacaaa ggtttcaaag ccaccgtatt tcgctggtaa ccctctgcta
     8701 ccaatgataa aaacatcctg cattgcatac taccccattc taaaatataa tcagtgatat
     8761 tataccacaa ttagaaggct aatgcgacct taaacccatg aaaaagagtt ggccttagaa
     8821 agccaactct ttttagaata cttacttttt cagtccttgt tggtaaaatg ccttcaaggc
     8881 ttcttgccat gttggaataa caaaccctgt tgcttttgcc ttatcaagat tcatggttga
     8941 atttaaagga cgtttggctt ttgctgggaa agctgatgaa tcaactggca ccacttcaac
     9001 agccttgtct tttaagattt ctttggcaaa atcataccaa gtggtgtctt ccttagcatc
     9061 atttgacaag tgatagtaac caaaggcttt ttgattctct gtcaaataac acatgaattc
     9121 tgccaaggtt cttgtccacg ttggccgacc gtgttggtca ttgacaacag tcaaacggga
     9181 gtgattttcc gctaactgct ccatggcaaa gacaaagttt ttaccataat ttccaaatac
     9241 ccaagcagta cggataatat aaaaatgctc tgcgtatcgt tcaacagcta gttctcctaa
     9301 acgttttgta cggccatatt ctgtcttagg gtctggacga tctgtttcta cccattcttg
     9361 accgactggt ttattgccat caaatacgta gtctgttgaa atgtaaacaa gggtagcccc
     9421 atattttcca caagccttgg caatattttc tgagcctgtt acattaatag cctcattcaa
     9481 ggcttttcct tcgtcttctg ctgcatcaac tgccgtataa gcagcacaat gatagaccaa
     9541 tgttggcttg acctgcgcaa aaacagcttc gactttgtct tcatttgtga tgtccatttc
     9601 tgcgacatca acggctacat aatcaacacc tcgttcatct aataagtatc gaagttctgt
     9661 tcctaattga ccattgcttc ctgtaattaa aatcatcagt tctccttttt gccataggta
     9721 gaacactttt ctcagtatct tagtgttctc taacattaac caaaggcttt cacctgtatt
     9781 tccttttatt ctacatcaaa aaagggaata tttcatccct ttactatcaa tgattagcga
     9841 atacccagcg caatcctagc atagcggctc attttatcaa ccgtccaagc agggtaccag
     9901 actaatttga cctcagtctt agtgacttct ggcacatctt gcagggcatc atggatgtaa
     9961 tctgtcaaca agtctgccaa aggacaaccc attgttgtga gtgtcatatc aatctctgta
    10021 taaccgttat cattgaaccg aatttcataa atcaaaccta gattaacaac atcaatacct
    10081 agctcgggat caataacagt ttctaaagct tcaagaattc tattcttgat agctatcacc
    10141 tgatcttgtg tatattttgg tgtatcagac atggctttcc tttcattttt tgaatatgtc
    10201 gtagataagt aaaaaaacca ctaaagcttt gcctatttag aggaattgcc ctctgggctt
    10261 gtttaagata tcaagtccaa ccttaaaaat acccaccaca ttcggtccga tttctaattt
    10321 gtaggtgtac cgctgataag tgccacttag gattttcgct cctttcttat ttcaaaggaa
    10381 cgacctaaaa tcgtccactg gacgatttta gtcctctata aagtctctta attgtttact
    10441 acgactagga tggcgaagtt ttctaagggc tttagcttca atctgtcgaa tgcgctcacg
    10501 agtaacatta aagactttac ctacgtcctc taacgtacgc attttaccat cgtcaaggcc
    10561 aaagcgtagg cgaagaacgt tctcttcacg gtcagtaagc gtatctaaaa cttcatccaa
    10621 ttgctcacga agaacaacac gcgtcgtata atctactgga ttttcaataa cttcatcttc
    10681 gataaaatca cctaagtgac tatcatcttc ttccccaatt ggtgtttcta aagaaacagg
    10741 ttcttgagca atttttagaa tttcacgaac cttgtctgga gtcatttcca tacgttctgc
    10801 gatttgttct ggcgttggat cttgaccaag ttcttgaaga aggttacgtt gttcacggac
    10861 taatttatta atcgtttcaa ccatatgaac agggatacga atcgtacgtg cttgatctgc
    10921 aattgcgcgt gttatagctt gacgaatcca ccatgttgca taagttgaga atttgaatcc
    10981 ttttgaataa tcaaatttat caacagcttt catgagtccc atgttacctt cttgaattaa
    11041 atcaagaaat tgcattccac gtccaacata acgttttgca attgagacaa caagacgaag
    11101 gtttgcttcc gctaaacgtt gtttagccat taaatctccc ttggcaactg caacagccaa
    11161 ttctttttcc tcttcacttg ttaaaagcgg taccacacca atttctttga ggtacatgcg
    11221 gacagggtcg tttactttag ctgaattact accaattaat tcttcatcag ttaattcttc
    11281 tggttttggt tcttcaacaa tgtattttga tgatgggtta ccttctttat ccgtaatcga
    11341 gataccacca tctgttaacc gctcaagtaa atcatcaatc tgatcagcat ccaaagcaaa
    11401 tggaatgaca agtttttcgg tcacgtcatc atcaatcgct gttccttctt ttttgtgatg
    11461 gcgaataaac tccgcaactt gaacgttaaa agttgttatt tctttttgtt ttgtcataat
    11521 ttcctctatt ccatttttcg tttctgcgca ataaaatgtt ctagtacttc tagagccgct
    11581 tgatgatcgc ctttgttact agattctcta acttttttcc cttgtttgtg aagatcgcgc
    11641 tctgctaaaa gtttggcacg tttggataaa atatcatcaa tctcaccaag agctacttct
    11701 ttgggaaggt tttcttctaa aacattgtaa taagcacggt taacttcctc tgacatctct
    11761 gacaaatcat aagatgtaat gtatccttgt tgcttcagcc gttgataaag tatttctaag
    11821 gtagaggtat caaaataaaa atcatcacga tgtcgaaatt catttaataa atagtcatga
    11881 tgtaagagcc gatgcatgag atgactttct gtcttagcaa tagctgtcaa actttttggt
    11941 attggtaagg tcacaagatt gctgacggct tgagctattt gaccttgacg cttttgccta
    12001 tcttgaatcc ttaatgcatt tactgattgt tctacttgaa aatagtcaaa gtttggcaac
    12061 aaatcagcaa tcttgttaat atacgaatgt tgagctgtga tggatggtga ttgagcaatc
    12121 aatggtgcca ttttctccac ataaacaatt tgtgattgca gattgtctac attagtaggt
    12181 tttaggtaat caataaaaaa ttcaacacta ctgatccgtg actgcttaag caaatctgca
    12241 aatgcttctg gggaatgccg ttgtacaaat tcgtcaggat ccattttatt ggggattctg
    12301 acaatttcga caacaaaatc tttaagcaat tctagtgatt ttgcaatagc atgttgtcca
    12361 gcatcgtcac catcataaat caaaacaact tttttagtga cttgcttaag gtgattgaca
    12421 tgttcttgag tcaatgccgt ccccattgaa gcaacagcat tttcatagcc ggaacggtaa
    12481 gcggcaatca cgtccataaa cccttccatt agaaacactt catgggtttt ggcaataaca
    12541 ggccttgctt tgtccagatg atacaattca taagatttgt taaaaagaac tgttcctctt
    12601 gaatttttat acttggcctg tctcttttcc atatcagctg ccgtccagat acgtcctgaa
    12661 aaggcaataa tatgccctcg gtcatctgat aagggaaaca tgatacgatt tcgaaaggcg
    12721 tcgtaaatgg tattggattg atcggacaag ttaaacaatc ctgaagcaac caattgacct
    12781 tcctcgtatt ttttagaaag agcttgataa agataatctg actcatctgg ggctaaacca
    12841 atattgaaat gctcaattaa ttggtcatcc aagcctctct ggtaaaggta cttcctagct
    12901 tcttgaccaa tggtagtggt catcaaaact gcatggtaaa atttagcagc atcctcatga
    12961 agtgtcatca aagcgtgatt agggtgcttg tgttggctag caagtactgc ctgacttggc
    13021 ggtatattaa gcgacatacc agtcttatcg gcaataatct gaacactttc taagaagggg
    13081 acttggcggt attcctcaat aaatttaaaa acatcccctg attttccaca gccaaagcag
    13141 tgaaaaaatt gtctgtcttc aacaacattg aaagagggtg tcttttcctt atgaaatggg
    13201 caaagcccga gataatgccg ccctgatcgg gaaagtttga ccacttctcc aatgacatcg
    13261 acaatattaa cgctattttt tacttgggaa atcatttctt tgtcaattgc caaatcgtca
    13321 cctccccata aaaatcccat tctagtttta cgttttatta taccaccgaa actctttttt
    13381 gtaaatttaa aaactgaagt gattccattg gattttgttt actaattatg ctatgcttaa
    13441 cttagagata ttaaaagggg gaatatttaa tggttaaaga attaaaagca tttttgttta
    13501 gaggaaacat tattgaactt gctgtagcag ttatcatcgg gggagctttt ggtgctattg
    13561 tcacttcttt tgtgaacgat attatcacac cgcttatttt aaacccagct ctgaaggccg
    13621 caaatgttga aaacatcact caattgtcat ggaatggtgt taaatatggt agctttttag
    13681 gagccgttat taattttctt atcatcggta ccagtctttt ctttgttgta aaagccgctg
    13741 aaaaagctat gcctaaaaaa gaaaaagaag ctgcagctcc tactcaagaa gaattattga
    13801 cagaaatccg tgatttgttg gctcaaaaat aaagcgattt ttgataaagt agttaggcat
    13861 gtctaactgc tttttttgat atcaaaaaag ctcccttgct tcttctggat aaaaaataaa
    13921 aacagttcta attgtgagaa ctgtttttat tttttaccat tagaattttt tacgtttgcg
    13981 agctgcttct gatttacgtt tacgttttac agaaggtttt tcgtagaatt cacgtttacg
    14041 tgattcttga agagtaccag ctttagtaac agaacgtttg aagcgacgaa gagcatcgtc
    14101 aagagattca ttcttacgta ctactgtttt tgacatgtgt ttcacctcct caagatattg
    14161 taacattttc acgttcccaa tt
//
LOCUS       DATXIG010000039        13337 bp    DNA     linear   BCT 28-DEC-2023
DEFINITION  TPA_asm: Streptococcus pyogenes strain MGAS11113
            SAMN08555799-rid19682583.denovo.039, whole genome shotgun sequence.
ACCESSION   DATXIG010000039 DATXIG010000000
VERSION     DATXIG010000039.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN08555799
            Sequence Read Archive: SRR6740495
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pyogenes
  ORGANISM  Streptococcus pyogenes
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 13337)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13337)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-OCT-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-OCT-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001918058.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001918058.2
            Genome Coverage       :: 368x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/12/2023 11:23:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 1,806
            CDSs (total)                      :: 1,769
            Genes (coding)                    :: 1,722
            CDSs (with protein)               :: 1,722
            Genes (RNA)                       :: 37
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1, 1 (5S, 23S)
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 47
            CDSs (without protein)            :: 47
            Pseudo Genes (ambiguous residues) :: 0 of 47
            Pseudo Genes (frameshifted)       :: 30 of 47
            Pseudo Genes (incomplete)         :: 20 of 47
            Pseudo Genes (internal stop)      :: 9 of 47
            Pseudo Genes (multiple problems)  :: 11 of 47
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13337
                     /organism="Streptococcus pyogenes"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN08555799-rid19682583.denovo.039"
                     /strain="MGAS11113"
                     /serotype="Emm28"
                     /isolation_source="normally sterile site"
                     /host="Homo sapiens"
                     /db_xref="taxon:1314"
                     /country="Finland"
                     /collection_date="2002"
                     /collected_by="National Public Health Laboratory"
     gene            complement(102..407)
                     /locus_tag="VC122_001232"
     CDS             complement(102..407)
                     /locus_tag="VC122_001232"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011681733.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1292 domain-containing protein"
                     /protein_id="HEP5641156.1"
                     /translation="MTHNHENDHQHEVITLVDEQGNETLFEILLTIDGREEFGKNYVL
                     LVPAGSEEDESGEIEIQAYSFTENEDGTEGDLQPIPEDSDAEWDMIEEVFNSFLDEN"
     gene            complement(419..838)
                     /gene="ruvX"
                     /locus_tag="VC122_001233"
     CDS             complement(419..838)
                     /gene="ruvX"
                     /locus_tag="VC122_001233"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012561069.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Holliday junction resolvase RuvX"
                     /protein_id="HEP5641157.1"
                     /translation="MRIMGLDVGSKTVGVAISDPLGFTAQGLEIIKIDEEKAEFGFTR
                     LEELVKQYQVEQFVIGLPKNMNNTNGPRVDASITYGNHIEHLFGLPVHYQDERLTTVE
                     AERMLIEQADISRGKRKKVIDKLAAQLILQNYLNRNF"
     gene            complement(835..1104)
                     /locus_tag="VC122_001234"
     CDS             complement(835..1104)
                     /locus_tag="VC122_001234"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006738453.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IreB family regulatory phosphoprotein"
                     /protein_id="HEP5641158.1"
                     /translation="MGFTDETVRFKLDDGDKRQISETLTAVYHSLDEKGYNPINQIVG
                     YVLSGDPAYVPRYNDARNQIRKYERDEIVEELVRYYLQGNGIDVK"
     gene            complement(1218..1616)
                     /gene="spx"
                     /locus_tag="VC122_001235"
     CDS             complement(1218..1616)
                     /gene="spx"
                     /locus_tag="VC122_001235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017768833.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator Spx"
                     /protein_id="HEP5641159.1"
                     /translation="MIKIYTISSCTSCKKAKTWLNAHKLAYKEQNLGKEPLTKEEILA
                     ILSKTENGVESIVSSKNRYAKALDCDIEELSVSEVIDLIQDNPRILKSPILIDDKRLQ
                     VGYKEDDIRAFLPRSIRNIENTEARLRAAL"
     gene            complement(1907..3043)
                     /gene="recA"
                     /locus_tag="VC122_001236"
     CDS             complement(1907..3043)
                     /gene="recA"
                     /locus_tag="VC122_001236"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015912068.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase RecA"
                     /protein_id="HEP5641160.1"
                     /translation="MAKKLKKNEEITKKFGDERRKALDDALKNIEKDFGKGAVMRLGE
                     RAEQKVQVMSSGSLALDIALGAGGYPKGRIIEIYGPESSGKTTVALHAVAQAQKEGGI
                     AAFIDAEHALDPAYAAALGVNIDELLLSQPDSGEQGLEIAGKLIDSGAVDLVVVDSVA
                     ALVPRAEIDGDIGDSHVGLQARMMSQAMRKLSASINKTKTIAIFINQLREKVGVMFGN
                     PETTPGGRALKFYASVRLDVRGTTQIKGTGDQKDSSIGKETKIKVVKNKVAPPFKVAE
                     VEIMYGEGISRTGELVKIASDLDIIQKAGAWFSYNGEKIGQGSENAKRYLADHPELFD
                     EIDRKVRVKFGLLEESEEESAMVVASEEIDDLALDLDNGIEIED"
     gene            complement(3132..4403)
                     /locus_tag="VC122_001237"
     CDS             complement(3132..4403)
                     /locus_tag="VC122_001237"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011285266.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="competence/damage-inducible protein A"
                     /protein_id="HEP5641161.1"
                     /translation="MKAELIAVGTEILTGQIVNTNAQFLSEKMAELGIDVYFQTAVGD
                     NEERLLSVITTASQRSDLVILCGGLGPTKDDLTKQTLAKYLRKDLVYDEQACQKLDDF
                     FAKRKPSSRTPNNERQAQVIEGSIPLPNKTGLAVGGFITVDGISYVVLPGPPSELKPM
                     VNEELVPLLSKQYSTLYSKVLRFFGIGESQLVTVLSDFIENQTDPTIAPYAKTGEVTL
                     RLSTKTENQALADKKLGQLEAQLLSRKTLEGQPLADVFYGYGEDNSLARETFELLVKY
                     DKTITAAESLTAGLFQSTLASFPGASQVFNGGFVTYSMEEKAKMLGLPLEELKSHGVV
                     SAYTAEGMAEQARLLTGADIGVSLTGVAGPDMLEEQPAGTVFIGLATQNKVESIKVLI
                     SGRSRLDVRYIATLHAFNMVRKTLLKLENLL"
     gene            complement(4472..5032)
                     /locus_tag="VC122_001238"
     CDS             complement(4472..5032)
                     /locus_tag="VC122_001238"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002992183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-3-methyladenine glycosylase I"
                     /protein_id="HEP5641162.1"
                     /translation="MKRCSWVPKDNQLYCDYHDLEWGQPLHDDRDFFELLCLESYQSG
                     LSWLTVLKKRQAFRTVFHHYDIASVAAFTSEEMADALENPSIIRHKLKLAATVNNAIA
                     VQKIQEEFGSFSTYLWNFVGGKPINNLVNQENLVPAQTELSIRLAKDLKKRGFKFLGP
                     TTVYSFMQASGLVNDHEEACVFNRIF"
     gene            complement(5042..5638)
                     /gene="ruvA"
                     /locus_tag="VC122_001239"
     CDS             complement(5042..5638)
                     /gene="ruvA"
                     /locus_tag="VC122_001239"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015912071.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Holliday junction branch migration protein RuvA"
                     /protein_id="HEP5641163.1"
                     /translation="MYDYIKGQLTKITAKYIVVETNGLGYIINVANPYSFTDSVNQLV
                     TIYLHQVIREDAHLLFGFHTEDEKDVFLKLISVSGIGPTTALAIVAVDDNEGLVNAID
                     NSDIKYLMKFPKIGKKTAQQMVLDLAGKFVEAPQETGNTKARSNKAGNTQLDEAIEAL
                     LALGYKAAELKKIRAFFEGTSETAEQYIKSALKLLMKG"
     gene            complement(5640..6860)
                     /locus_tag="VC122_001240"
     CDS             complement(5640..6860)
                     /locus_tag="VC122_001240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010922756.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HEP5641164.1"
                     /translation="MQEFLNLPKQIQLRQLVRFVTITLGSSIFPFMAMYYTTYFGTFW
                     TGLLMMITSLMGFVGTLYGGHLSDALGRKKVIMIGSVGTTLGWFLTILANLPNAAIPW
                     LTFVGILLVEIASNFYGPAYEAMLIDLTDESNRRFVYTINYWFINIAVMFGAGLSGLF
                     YDHHFLALLVALLLVNVLCFGVAYYYFDETRPETHAFDHGKGLLASFQNYRQVFHDRA
                     FVLFTLGAIFSGSIWMQMDNYVPVHLKLYFQPTAVLGFQVTSSKMLSLMVLTNTLLIV
                     LFMTVVNKLTEKWKLLPQLVVGSLLFTLGMLLAFTFTQFYAIWLSVVLLTFGEMINVP
                     ASQVLRADMMDHSQIGSYTGFVSMAQPLGAILASLLVSVSHFTGPLGVQCLFVVIALL
                     GIYFTVVSAKMKKV"
     gene            complement(6871..8853)
                     /gene="mutL"
                     /locus_tag="VC122_001241"
     CDS             complement(6871..8853)
                     /gene="mutL"
                     /locus_tag="VC122_001241"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012678696.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA mismatch repair endonuclease MutL"
                     /protein_id="HEP5641165.1"
                     /translation="MTNIIELPEVLANQIAAGEVVERPASVVKELVENAIDAKSSQIT
                     VEIEESGLKMIQVTDNGEGMSHEDLPLSLRRHATSKIKSQSDLFRIRTLGFRGEALPS
                     VASISKITIKTATKEVTHGSLLIATGGEIETLEAISTPTGTKIKVENLFYNTPARLKY
                     MKSLQAELAHIVDVVNRLSLAHPEVAFTLISDGRQLTQTSGTGDLRQAIAGIYGLNTT
                     KKMLAISNADLDFEVSGYVSLPELTRANRNYMTILVNGRYIKNFLLNRAILDGYGSKL
                     MVGRFPIVVIDIQIDPYLADVNVHPTKQEVRISKERELMALISTAISESLKEQDLIPD
                     ALENLAKSSTRHFSKPEQTQLPLQFRGLYYDPQKNDFFVKESAVSEKIPETDFYFGAV
                     DNSVKVEKAELLPHSEEVIGPSSVKHASRPQNTFIETDHPNLDLKNRQKLSQMLTRLE
                     NEEQSVFPELDYFGQMHGTYLFAQGKDGLFIIDQHAAQERVKYEYYRDKIGEVDSSLQ
                     QLLVPYLFEFSGSDFINLQEKMALLNEVGIFLEVYGHNTFILREHPIWMKEEEIASGV
                     YEMCDMLLLTNEVSIKTYRAELAIMMSCKRSIKANHSLDDYSARNLLLQLAQCQNPYN
                     CPHGRPVLINFSKADMEKMFRRIQENHTSLRELGKY"
     gene            complement(8948..10099)
                     /locus_tag="VC122_001242"
     CDS             complement(8948..10099)
                     /locus_tag="VC122_001242"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003058754.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific integrase"
                     /protein_id="HEP5641166.1"
                     /translation="MIMKITEHKKKNGTIVYRASIYLGIDQMTGKRVKTSITGRTRKE
                     VNQKAKHAQFDFLSNGSTIKRKVVIKTFKELSHLWLETYKLTVKPQTYDATVTRLNRH
                     IMPTLGNMKVDKITASDIQMLINRLSKYYVNYTAVRSVIRKVLQQGVLLGLIDYNSAR
                     DIILPRKQPNAKKKVKFIDPSDLKSFLEHLETSQHKRYNLYFDAVLYQLLLSTGLRIG
                     EACALEWGDIDLENGTIAINKTYNKNLKFLSTAKTQSGNRVISVDKKTLRSLKLYQMR
                     QRQLFNEVGARVSEVVFATPTRKYFNASVRQSALDTRCKEAGIERFTFHAFRHTHASL
                     LLNAGISYKELQYRLGHANISMTLDTYGHLSKDKEKEAVLYYEKAMNNL"
     gene            10348..10491
                     /locus_tag="VC122_001243"
     CDS             10348..10491
                     /locus_tag="VC122_001243"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011185066.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative holin-like toxin"
                     /protein_id="HEP5641167.1"
                     /translation="MSGGGAYVCKSQKPKERRRQGLSVYETLTLMIAFGTLIVAIMNN
                     KNK"
     gene            complement(11353..12120)
                     /locus_tag="VC122_001244"
     CDS             complement(11353..12120)
                     /locus_tag="VC122_001244"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003058809.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HEP5641168.1"
                     /translation="MAKNSPQDLINREIFSTNLNMLMAKKNIKQIDIHNKLGIPKSTI
                     TGYVKGRSLPTAGNVQKLADFFGVLKSDIDPRFDSNNIETNSNIIPSTLQKVTSTLSQ
                     LEHKRQLNVLDYAETQLEQQNTVEEPQATYYTYNYYDHAASAGTGQYLNDVQVETIEL
                     PVDYDADFVIPVYGDSMEPEYHSGDYVFIKLSINLSDGDIGVFEYYGDAYIKQLVIND
                     SGAFLHSLNDKYDDILIDRDSDFRIIGEVIGSFTSKS"
     gene            12274..12480
                     /locus_tag="VC122_001245"
     CDS             12274..12480
                     /locus_tag="VC122_001245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002992503.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HEP5641169.1"
                     /translation="MTQITLKAARINAGYTLKQVAGAVGKNPQTISKYEKDSSDISLG
                     LLQKLSSLYGVTIDNLFLGKKVRK"
     gene            12514..12714
                     /locus_tag="VC122_001246"
     CDS             12514..12714
                     /locus_tag="VC122_001246"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002992502.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HEP5641170.1"
                     /translation="MTVGDKIKTIRLNLGETMEEFGKRFGTSKGTVNNWEKGRNLPNR
                     NSLLRIAQLANQTVSDFLAITD"
     gene            12735..13142
                     /locus_tag="VC122_001247"
     CDS             12735..13142
                     /locus_tag="VC122_001247"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011185070.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEP5641171.1"
                     /translation="MARTPFTQELLHQIFDDTGTMSLELIAERLPDWSEKDIKLRLAA
                     WRYRNNIDYTMANGEIDTFEIINNRKAISEEVSAGRQLKLEEYFKQVQATAEIINKPT
                     ASDTNRLKAIQLQQVAMDEIPDQYFKELTELYG"
BASE COUNT         4144 a         2714 c         2303 g         4176 t
ORIGIN      
        1 atttaattct tttatcatta tcacctcctg cttatttatt cgaaaataac attaaaaaaa
       61 tagccctcac ggctactttt taacatagtg tcactaacaa tttagttctc atctagaaaa
      121 ctattaaata cctcttcaat catatcccat tcagcatctg aatcctcagg aatcggttgt
      181 aaatcccctt cagtaccatc ttcattttca gtaaatgaat aggcttggat ttcaatttca
      241 ccagactcat cttcttcaga tcccgctgga accaagagaa cataattttt accaaactct
      301 tcacgaccgt caattgttaa taaaatttca aacaaggttt catttccttg ttcatccaca
      361 agtgtgataa cttcgtgttg gtggtcattt tcatgattat gtgtcatttt ttatctcctt
      421 aaaaattccg atttaaatag ttctgtaaaa tgagttgtgc agctaattta tcaatgactt
      481 tttttcgctt accacgacta atgtcagctt gctcaataag catacgctct gcctcaacag
      541 tcgtcaatcg ttcatcttga taatggactg gaagtccaaa gaggtgctct atgtggtttc
      601 cataagttat acttgcatca acacgtggac cattggtgtt attcatattt tttggcaatc
      661 caataacaaa ctgttcaact tggtactgct taacaagctc ttctaaacgt gtaaagccaa
      721 attctgcttt ttcctcatca atcttgataa tttcaagtcc ttgggctgta aagccaagag
      781 gatcactaat agctacacca accgttttcg agccaacatc aagtcccatt attctcattt
      841 aacatcaatt ccatttcctt gtaaatagta gcgaacaagt tcttctacaa tttcatcacg
      901 ttcatattta cgaatctgat ttcgagcatc attataacga ggaacataag ctgggtcccc
      961 actcaagaca taaccaacaa tttgattaat tggattataa cccttttcat caagtgaatg
     1021 atagactgct gttaatgttt cactaatttg tcgtttgtcg ccatcatcca acttaaagcg
     1081 gactgtttca tctgtaaatc ccatacttac accttctttc taaaattaag tgctatgctt
     1141 tattatagct tatttctatt aaataaacaa gaatttcaag cgaagaaggt atttaaaaaa
     1201 aagctctaca agagctttta gagtgcagca cgtaatcgtg cttcagtatt ttcaatatta
     1261 cgaatagagc gaggtaaaaa agcacgaata tcgtcttctt tgtagcctac ttgcagacgt
     1321 ttatcatcaa tcaaaatggg acttttaagg atgcgcgggt tatcttgaat caaatcaata
     1381 acctcactaa cacttagttc ttcaatatcg caatcgagag ctttggcata acgattttta
     1441 gatgaaacaa tgctctccac tccattttcg gtttttgata aaattgctaa aatttcttct
     1501 ttagttagcg gttcttttcc taagttctgt tctttataag caagcttatg ggcatttagc
     1561 caagttttgg ctttcttgca gctcgtacaa cttgaaatcg tgtaaatttt aatcatgttg
     1621 ctgactcact ttctatttcg tgatagcttt attgtagcac agtttcttat ctataaatat
     1681 aggtctttgg acctattttt ttaattttaa agatattatc ttggtaaaat aaggtttttc
     1741 acctgtattc ccttgaatta ttagttattt taattttata tcagttaacc taagttctgc
     1801 tctctcacat ctttttaaat gatggtttct attcacccac acacctatga atagtagtcc
     1861 tactaacctt taaaaataca ttttaaaaag ccctgaaggg ctctttttaa tcttcaattt
     1921 caataccatt atctaaatct aaagcaagat catcgatttc ttctgatgct actaccatag
     1981 cagattcttc ttcgctttct tcaagcaaac caaatttaac acgtacttta cggtcgattt
     2041 catcaaacaa ttctggatga tcggccaaat aacgcttagc attttcagaa ccttggccaa
     2101 tcttctcacc attataagag aaccaagcac ctgctttttg gataatgtcc aaatcagaag
     2161 caattttcac aagctcccct gtacgagaaa taccttcccc atacatgatt tcaacttctg
     2221 ctaccttaaa tggcggagcg accttgtttt taacaacctt gattttggtc tccttaccaa
     2281 tgctgctatc tttttggtca ccagttcctt taatttgagt tgttccacgc acatccaacc
     2341 gaacagaagc atagaatttc aaagctcgac cacctggtgt tgtttcagga tttccaaaca
     2401 tgacaccaac tttttcacgc aattggttaa taaagatagc gatagttttt gttttattaa
     2461 tagaagctga taatttacgc atagcctgac tcatcatacg tgcttgcaat ccgacatggc
     2521 tatcgccaat atcaccatca atttcagcac gtggcactaa agctgctact gaatcgacaa
     2581 caaccagatc aaccgcacca gaatcaatca atttacctgc aatttcaagt ccttgttctc
     2641 cagaatctgg ttgagacaag aggagttcat caatattaac cccaagcgca gcagcataag
     2701 ctggatcaag cgcatgctcg gcatcgataa aggctgcgat tccaccttct ttttgagctt
     2761 gtgctacagc atgtaaagcc acagtcgttt taccggaaga ctctggacca tagatttcga
     2821 tgatacgtcc tttaggataa ccaccagctc caagcgcaat atcaagagct agacttcctg
     2881 aactcataac ctgaactttt tgctctgcac gttctcctaa tcgcatcact gcgcccttac
     2941 caaaatcttt ttcaatattt ttcaaagcat catcaagggc tttgcgacgt tcgtcaccaa
     3001 acttcttagt gatttcttca ttctttttta atttttttgc caatactttc tcctcttttt
     3061 taacaaacca tcgtcagcag gattaatcta cataatcgta aaccatatcc tactatagct
     3121 actaaataac attatagcaa attctcaagt tttaataaag ttttacggac catattaaag
     3181 gcatgtaaag tagcgatata gcgcacatcc aaacgacttc gcccgctaat caaaaccttt
     3241 attgattcta ccttattttg agtggcaagg ccaatgaaaa ctgtacctgc aggctgttcc
     3301 tccaacatat ctggtccggc aacacctgtt aaacttaccc caatatcagc accagtcaat
     3361 aaccttgctt gctccgccat cccctcggct gtataagcac taacaacgcc atgcgatttc
     3421 aactcctcta aaggaaggcc tagcattttc gctttttctt ccatgctata agtcacaaag
     3481 cctccattga atacttgaga agctcctgga aaactcgcca aagttgactg aaataatccc
     3541 gcggttagac tttctgctgc tgtaattgtc ttatcatatt ttactaagag ctcaaatgtc
     3601 tcacgcgcta aggaattatc ctccccatag ccataaaaga catcagctaa gggttgacct
     3661 tcaagagttt ttcgggatag tagctgcgct tctagctgac ctaacttttt atctgccaga
     3721 gcttggtttt cagtttttgt tgataagcga agagtcactt cgccagtctt agcatacgga
     3781 gcaatggttg gatcagtttg attctcaata aaatctgaca agactgttac caactgactt
     3841 tccccaatac caaaaaagcg tagtaccttt gaatacaatg tactgtattg ttttgacaga
     3901 agtggtacca attcttcatt taccatcggc ttcaattcac ttggaggacc cggtaagaca
     3961 acataactaa taccatcgac tgtgatgaac ccaccaaccg caagaccagt tttatttggc
     4021 aaagggattg acccttcaat cacttgtgcc tgtcgctcat tatttggtgt ccgtgatgaa
     4081 ggcttgcgct tagcaaaaaa gtcatctagt ttctgacaag cttgctcatc ataaaccaag
     4141 tctttcctaa ggtactttgc taaagtttgt ttggttaaat catctttcgt tggaccaagg
     4201 ccaccacata aaattaccaa gtcactacgc tgactagcag ttgtaatcac tgaaagtaaa
     4261 cgctcctcgt tgtccccaac agccgtttga aaatagacat caatacctag ctctgccatt
     4321 ttttccgaca gaaattgagc attggtattc acaatttgac cagtcaaaat ttcggtacct
     4381 actgcaatca gttcagcttt catttcccca cctacttatc tattcgtaat ctgatctcat
     4441 tttatcaaaa aatcttccaa ttcacgaaga attagaagat tctattgaaa acacaagcct
     4501 cttcatggtc atttaccaga cccgatgctt gcataaaaga ataaaccgtt gtaggcccta
     4561 aaaatttaaa tcctcttttc tttaaatcct tagctaagcg aatagataat tctgtttgag
     4621 caggaacaag attctcttga ttgacaaggt tgttaattgg ctttccccca acaaaattcc
     4681 agagataagt tgaaaaacta ccaaactctt cttgaatttt ctgtactgcg atagcattgt
     4741 tgacagttgc tgctagcttt agcttgtggc gaatgatgga tggattctct aaagcgtcag
     4801 ccatttcctc cgaggtaaag gctgcaaccg atgcaatgtc gtaatgatga aaaacagttc
     4861 tgaaagcttg gcgttttttc aaaacagtca gccaggataa gcctgactgg taactttcaa
     4921 ggcagagtaa ttcaaaaaaa tctctatcat catgtagggg ttgaccccat tctaaatcgt
     4981 ggtaatcaca gtaaagctgg ttatccttag gaacccatga acaacgtttc atgtgaaaca
     5041 cctagccttt cattaatagt ttaagggctg atttgatgta ttgttcggcc gtttcagagg
     5101 ttccttcaaa gaaggcacga attttcttca attccgctgc cttataaccg agggcaagga
     5161 gggcttcaat cgcttcgtcc agttgagtat tgcctgcttt attgctgcgc gctttggtat
     5221 taccagtctc ttgtggagcc tccacaaatt tgccagctaa gtcaagaacc atttgctgcg
     5281 ctgttttttt accaatttta ggaaatttca ttaggtactt aatgtcactg ttatcaatgg
     5341 cattcacaag tccctcatta tcatcaactg ccacaatagc aagagctgtt gtggggccaa
     5401 tgccagatac agaaattaat ttcagaaaaa catctttttc gtcttccgta tgaaacccaa
     5461 acaacaagtg agcatcctca cgaatcactt gatgcaggta aatagttacc agttggttga
     5521 cactatctgt aaagctataa ggattggcta cattgataat atagcctagt ccatttgttt
     5581 caacgacaat gtattttgcc gtaattttgg tcaattgacc tttaatataa tcgtacatac
     5641 tacacctttt tcatttttgc agaaacaacc gtaaaataaa tccctagcaa agcaatgact
     5701 acaaataagc attgcacacc taaaggacct gtaaaatggc tgacagatac tagtagacta
     5761 gccaaaatag cacctagggg ttgtgccatt gacacaaaac ctgtataaga tcctatttgg
     5821 gaatgatcca tcatatcagc acgtaggact tgactagcag gaacatttat catttcccca
     5881 aaagttaaca aaacaactga tagccaaata gcatagaact gcgtaaaggt aaatgccaag
     5941 agcatcccta gagtaaatag taaagaacca accacaagct gaggtaatag tttccatttt
     6001 tccgttaatt tatttactac tgtcatgaaa aggacaatca gcaatgtatt agttaaaacc
     6061 attaatgata acattttaga actagttact tggaaaccta acacagccgt tggctgaaaa
     6121 tacagtttca aatggactgg cacatagtta tccatctgca tccaaatact accagaaaag
     6181 atggcaccta aggtaaacaa gacaaaggca cgatcatgaa acacctgacg gtagttctga
     6241 aaactcgcca gtaatccttt accatggtca aaagcgtgtg tttctggtct agtctcatca
     6301 aaatagtagt aagcaacgcc aaaacaaagt acattaacga gtaataaggc tactaacaag
     6361 gctaaaaaat gatggtcata aaaaagccca gatagccctg caccaaacat gacggcaata
     6421 ttgataaacc aataattgat ggtgtaaaca aatcgacgat tactctcatc agtcaaatca
     6481 attaacatag cttcataggc aggaccataa aaattagaag caatctctac caataaaata
     6541 cccacaaagg ttaaccaagg aatagctgca ttaggcaaat tagctaaaat agtcagaaac
     6601 cagcctagtg ttgttcctac tgacccaatc ataatgactt ttttgcgacc aagagcatct
     6661 gacagatgcc caccgtataa agttccaaca aatcccatca aactggtaat catcattaag
     6721 aggcctgtcc aaaacgtacc aaagtaagtc gtataataca tggccataaa gggaaaaata
     6781 ctactgccta aggtaatggt cacaaagcgt accagttgcc tcagctgaat ctgcttagga
     6841 agatttaaaa actcttgcat ctcatcctct ctaatatttt cctagctctc gcaggctagt
     6901 gtgattttct tggatacgac ggaacatttt ttccatatct gccttactaa aattaatcaa
     6961 tacgggtcta ccatgagggc agttataagg attttgacat tgggccaatt gcagtagcag
     7021 atttcttgct gaataatcat ccaagctatg atttgccttg atagatcgtt tacaactcat
     7081 cataatagct aactctgctc ggtaagtttt aatagatacc tcattggtta gaagtaacat
     7141 gtcacacatt tcatagacac cagatgcaat ctcttcttct ttcatccaga taggatgctc
     7201 cctcaaaatg aaagtattgt gcccataaac ttctaagaag ataccaactt catttaagag
     7261 tgccattttc tcctgtaagt taatgaaatc agaaccagaa aactcaaaca aatagggcac
     7321 caacaattgc tgtaaactac tgtcaacctc acctatctta tcacgataat attcgtattt
     7381 aacccgctct tgagcagcat gctgatcgat aataaataag ccatctttac cttgagcaaa
     7441 gagataagtt ccgtgcatct gaccaaaata atccaattcg ggaaacactg attgctcttc
     7501 attttccaaa cgggttagca tttgagataa cttttgtctg ttttttaagt caagattagg
     7561 atgatcagtc tcgataaagg tattctgggg acgacttgcg tgtttaaccg aagaaggtcc
     7621 tataacttct tctgagtggg gtaacagctc tgctttttca acctttacac tgttgtcaac
     7681 agcgccgaaa taaaaatcag tttcaggtat tttttccgag acagccgact ctttgacaaa
     7741 aaagtcattc ttttgaggat cataataaag tcccctaaac tgtaaaggaa gttgtgtttg
     7801 ctctggttta gagaaatgtc gtgtgcttga tttggctaag ttttccagag catctggaat
     7861 caaatcttgt tccttaagac tttcagaaat cgccgtacta attaaagcca tcaactcacg
     7921 ctcttttgaa atacgaacct cttgttttgt gggatgaaca ttgacatcgg ccaaataggg
     7981 atcaatctga atatcaatca caacaattgg gaaacgtcca accatgagct tagaaccgta
     8041 accatcaaga attgctcgat taagcaagaa attcttgatg taacgcccat taaccaaaat
     8101 cgtcatgtaa ttacggttgg cgcgtgtcaa ctctggcaag ctaacatagc cggaaacttc
     8161 aaaatccaaa tcagcattag agatagccag catttttttg gtagtattca aaccatagat
     8221 ccctgcaatt gcttggcgta aatcgccagt tcctgatgtc tgagttaatt ggcgaccatc
     8281 actaatcagt gtgaaagcaa cttctggatg tgccaaactc aaccggttga ccacatctac
     8341 aatgtgagct aattctgcct gtaaactttt catatatttg agacgagcag gcgtgttgta
     8401 aaaaaggttt tcaaccttaa ttttggttcc agtaggagtt gagatcgctt caagtgtctc
     8461 aatttcccca ccagtagcta taagaagaga accgtgagtg acttcttttg ttgctgtttt
     8521 tatcgtgatt ttactgatag aggcaacaga cggtaaagcc tctccacgga agccaagtgt
     8581 tctaattcta aacaaatcac tctgactctt aattttacta gtagcgtggc gacgcagact
     8641 taaaggtaaa tcttcatgag acattccctc accgttgtct gtaacctgta tcatcttaag
     8701 gccagactct tcaatttcaa cggtaatctg gctactttta gcatcaatag cattctcaac
     8761 caattccttg acaacactcg ctggcctttc tacaacttca ccagctgcaa tttggttggc
     8821 gagaacttcc ggtaattcaa taatgtttgt cataattttg tttttaacac tttctaaacg
     8881 ttgatataac aaggtttcat taggtttaca cctctcaaaa tattaatttg tggacttttt
     8941 tgtggactta taaattattc atagcctttt cataatataa aacagcttct ttttctttgt
     9001 ccttagaaag atggccatag gtatccaaag tcatgctgat attcgcatgt cctagacggt
     9061 actgaagttc cttataacta atacctgcgt tcagcaataa actagcgtga gtgtgtctaa
     9121 aagcgtgaaa ggtaaagcgt tcaatccctg cttccttaca cctagtatct aaagcgcttt
     9181 gtctaaccga agcattaaaa tactttcgtg ttggtgtggc aaacactacc tccgacacac
     9241 gcgcaccaac ctcattaaat aattgtcgct gtctcatttg atagagcttt aggcttctaa
     9301 gggtcttttt atcaacacta atcactctat tgcctgactg ggttttagct gtactcaaaa
     9361 acttcaaatt tttattgtaa gtcttattaa tggctattgt accattttct aggtcaatat
     9421 ctccccactc taatgcacag gcttcgccta tcctcaagcc agtggataat aaaagttggt
     9481 agagaactgc atcaaagtaa aggttatagc gtttgtgttg actagtttct aaatgttcta
     9541 aaaaagattt caaatcagac ggatcaataa acttaacttt tttcttagcg tttggctgct
     9601 tccttggaag gataatatct cttgctgagt tataatctat tagccctagc aatactcctt
     9661 gttggagaac ttttcggatg actgaacgta ccgcagtata attgacgtaa tatttagata
     9721 atctattaat cagcatttga atatcactag cggttatctt atcaaccttc atattgccca
     9781 gagttggcat aatatgtcga ttaagtctag taacagtagc atcataagtt tgaggcttta
     9841 ctgttaactt ataggtttca agccataaat gactaagttc tttaaatgtt ttaatcacaa
     9901 cttttctttt aattgtagat ccattagata ggaagtcaaa ctgcgcgtgc ttggcttttt
     9961 gattaacttc ttttcttgtt cttcctgtga tgcttgtttt tactctctta cctgtcattt
    10021 ggtcaatgcc tagataaata ctagcacgat aaacaattgt accgtttttc ttcttatgtt
    10081 ctgttatttt catgatcata aacctttcca tcagcaggca agctattatt aaaagggttt
    10141 tagattagtt catatcatgg tataataatg gtatcgggta aatatccgaa atattataaa
    10201 agaaaatcat ggtataatat tagtcttttt aagaccttgc gagcgtggaa aacttgcgtg
    10261 gtcttttttg tttggttttg taaggtacag agagtttcag aagaagtttt gcatgtctaa
    10321 aaactaggtc aaggtagtcg tagccttatg tctggaggtg gtgcttatgt atgtaagtca
    10381 cagaaaccaa aagaaaggag acgacaaggt ttgtcagttt atgaaacgct tactttgatg
    10441 attgcctttg gtactctgat agtagctatc atgaataaca agaacaaata gaaaaaccgt
    10501 cccacacttt agccaatcgt ggacggttta tagaaaatat aaacaaatac ggtaaggctg
    10561 ccgtcttaaa cggtgctacc acaggagatt gtttgcgcaa tctccttttt ctatgtccat
    10621 tatagctttg tttgataaag aagtcaaaag ggtgtagtta gaaagtacag tctttagcgc
    10681 tcttaacatt ccttagtatt accaaaatga ataaaaataa ttttatgcaa gaatttataa
    10741 tactttatgc aaccactgat cacgcgcaga tagttttttt aattaccgcc gacaaaaagt
    10801 tcagaaaagt tcagattata cctcaatttt tgccaagttt tgagggtctt gtaattaaag
    10861 aaaattaaag gcgtatcaag gtatttgcgc gtaggtaaag atgataaatt tggcatttag
    10921 acatgaaaat tgaacaggtc ataaaatgag cggtaacggt aaggttacag taaggtagct
    10981 aattttattc cacgcgcata atgctgaact tcgagcgaac ttagagaata gaaggttact
    11041 actcccaaac acgaacacat ggtcgcattt cggaatgctg gaaaatgttg gcacgtactg
    11101 acataatacg tcaacctatt tcaatagttg taaaacgctc ggtaaattca cttaaagaac
    11161 ttgatatatc ggtcgtttat taatttactt tttccgttgt gacagcttct aaaatcagtt
    11221 ggcattttta gcgtatagta ttcagaaaat cggacttttc ggctgtctta aagagcaaaa
    11281 tgcgagtcct taaaggtaaa gcaaccaaaa ttagtggaaa atagtggaat tcggcgcgtg
    11341 ggtatcagat agtcatgatt tagatgtaaa actcccaata acttctccga taatacggaa
    11401 atcactatct ctatctatga gtatatcgtc atacttgtcg ttcagactat gcagaaacgc
    11461 tccagaatcg tttataacaa gttgtttgat ataagcatca ccgtaatact caaaaactcc
    11521 tatatcacca tctgacaggt taatagatag tttgataaat acatagtccc cagaatggta
    11581 ttcgggttcc atagaatcgc cataaaccgg tatgacaaaa tcagcgtcgt aatcgactgg
    11641 taattcaatt gtttctactt gtacatcatt tagatactga cctgtaccag ctgaagctgc
    11701 gtggtcgtag tagttgtaag tgtagtaggt ggcttgtggt tcttctactg tgttttgttg
    11761 ttctaattgt gtttcagcat aatcaaggac gtttaattgt cgcttgtgtt ctagctgaga
    11821 taaagtagat gttacttttt gtagggttga tgggataata ttactatttg tttcaatatt
    11881 attagaatca aaacgggggt caatgtctga ttttagaact ccgaagaagt ccgccagctt
    11941 ttgaacgttt ccagcagttg ggagtgaacg acctttaaca tagccagtta tcgtactctt
    12001 aggtattcct agtttgttgt gaatatctat ctgtttgata ttctttttag ccataagcat
    12061 gttgagattt gttgagaaaa tttctctatt tattaaatct tggggactat ttttagccat
    12121 ttctcttacc cctttctttt tgtatctgaa ttaattctaa cacaaataaa cctaaaagta
    12181 ctattttttt aatatttttc tacaaaaatc attgacttgg tacgaaaata atagtactat
    12241 taagccatag ctttttagaa aggagttgaa aacatgacgc aaattacact aaaagctgcc
    12301 cgaatcaacg ctggttacac tttaaaacaa gtagctggag cggttgggaa aaatccccaa
    12361 actatttcaa agtacgaaaa agatagctcc gatatttctt taggactatt gcaaaaatta
    12421 tcatctttgt acggtgtcac tattgataac ctttttttag gtaaaaaagt acgaaaataa
    12481 tagtacttta ttaattagaa aggcaggcaa gaaatgacag tgggtgacaa aatcaaaact
    12541 attcgcctta acttaggaga aactatggag gaattcggaa aacgctttgg cacttctaaa
    12601 ggtactgtta ataattggga gaaaggaaga aacctcccga atagaaacag tctattaaga
    12661 atagcacagc tcgctaatca aactgttagc gacttcttag ctatcaccga ttaagaaagg
    12721 caggtaaata acaaatggct agaacaccat ttacacaaga attattacat cagatatttg
    12781 acgatacagg aacaatgagc ctagagctga ttgctgagcg gcttcccgat tggtctgaaa
    12841 aagacatcaa gcttaggctg gcagcttggc ggtatcgaaa taacattgat tacaccatgg
    12901 caaacggtga aattgatacc tttgaaatta tcaataacag aaaagccata tccgaagaag
    12961 tttccgcagg aaggcaactt aagcttgaag agtatttcaa gcaagtacag gcaacggctg
    13021 aaattattaa taaaccgact gctagcgaca ccaatcgttt aaaggccatt caattacaac
    13081 aagtggctat ggatgaaatt cctgatcagt attttaaaga actaacggaa ctctatggat
    13141 aaaggaggat taccatgaac ctagtttaca tggatggtaa gaaagagcct tacaccacac
    13201 acgacatcat agctgaacat gctgaaattg acattatttc agttagaaag ctgattgata
    13261 agcataaaaa ggattaagaa cgaaagtgct gttgtgtggt acataaggta acgcaccccc
    13321 ttttttacta cgtaacc
//
LOCUS       DATXIG010000040        13102 bp    DNA     linear   BCT 28-DEC-2023
DEFINITION  TPA_asm: Streptococcus pyogenes strain MGAS11113
            SAMN08555799-rid19682583.denovo.040, whole genome shotgun sequence.
ACCESSION   DATXIG010000040 DATXIG010000000
VERSION     DATXIG010000040.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN08555799
            Sequence Read Archive: SRR6740495
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pyogenes
  ORGANISM  Streptococcus pyogenes
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 13102)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13102)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-OCT-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-OCT-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001918058.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001918058.2
            Genome Coverage       :: 368x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/12/2023 11:23:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 1,806
            CDSs (total)                      :: 1,769
            Genes (coding)                    :: 1,722
            CDSs (with protein)               :: 1,722
            Genes (RNA)                       :: 37
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1, 1 (5S, 23S)
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 47
            CDSs (without protein)            :: 47
            Pseudo Genes (ambiguous residues) :: 0 of 47
            Pseudo Genes (frameshifted)       :: 30 of 47
            Pseudo Genes (incomplete)         :: 20 of 47
            Pseudo Genes (internal stop)      :: 9 of 47
            Pseudo Genes (multiple problems)  :: 11 of 47
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13102
                     /organism="Streptococcus pyogenes"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN08555799-rid19682583.denovo.040"
                     /strain="MGAS11113"
                     /serotype="Emm28"
                     /isolation_source="normally sterile site"
                     /host="Homo sapiens"
                     /db_xref="taxon:1314"
                     /country="Finland"
                     /collection_date="2002"
                     /collected_by="National Public Health Laboratory"
     gene            66..1289
                     /locus_tag="VC122_001248"
     CDS             66..1289
                     /locus_tag="VC122_001248"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014407986.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="trypsin-like peptidase domain-containing
                     protein"
                     /protein_id="HEP5641172.1"
                     /translation="MPSMKYILKSLSILLIGFLGGLIAIITFNNLYPHSPSKINSGKA
                     TTSNMVFNNTTNTTKAVKAVQNAVVSVINYQDNPSSSLSNPYTKLFGEGRSKENKDAE
                     LSIFSEGSGVIYRKDGNSAYVVTNNHVIDGAKRIEILMADGSKVVGELVGADTYSDLA
                     VVKISSDKIKTVAEFADSTKLNVGEVAIAIGSPLGTQYANSVTQGIVSSLSRTVTLKN
                     ENGETVSTNAIQTDAAINPGNSGGPLINIEGQVIGINSSKISSTPTGSNGNSGAVEGI
                     GFAIPSTDVIKIIKQLETNGEVIRPALGISMVNLNDLSTNALSQINIPTSVTGGIVVA
                     EVKEGMPASGKLAQYDVITEIDGKTVNSISDLQSSLYGHDINDTIKVTFYRGTTKKKA
                     DIKLTKTTQDLTKTQ"
     gene            1348..2154
                     /locus_tag="VC122_001249"
     CDS             1348..2154
                     /locus_tag="VC122_001249"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011285341.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ParB/RepB/Spo0J family partition protein"
                     /protein_id="HEP5641173.1"
                     /translation="MFLILSRNSLMTKELLIDLPIEDIVTNPYQPRIQFNQRELQDLA
                     TSIKSNGLIQPIIVRKSDIFGYELVAGERRLKASKMAGLKKVPAIIKKISTLESMQQA
                     IVENLQRSNLNAIEEAKAYQLLVEKKHMTHDEIAKYMGKSRPYISNTLRLLQLPAPII
                     KAIEEGKISAGHARALLTLSDDKQQLYLTHKIQNEGLSVRQIEQLVTSTPSSKLSKKT
                     KNIFATSLEKQLAKSLGLSVNMKLTANHSGYLQISFSNDDELNRIINKLL"
     gene            2387..3742
                     /gene="dnaA"
                     /locus_tag="VC122_001250"
     CDS             2387..3742
                     /gene="dnaA"
                     /locus_tag="VC122_001250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012657571.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chromosomal replication initiator protein DnaA"
                     /protein_id="HEP5641174.1"
                     /translation="MTENEQIFWNRVLELAQSQLKQATYEFFVHDARLLKVDKHIATI
                     YLDQMKELFWEKNLKDVILTAGFEVYNAQISVDYVFEEDLMIEQNQTKINQKPKQQAL
                     NSLPTVTSDLNSKYSFENFIQGDENRWAVAASIAVANTPGTTYNPLFIWGGPGLGKTH
                     LLNAIGNSVLLENPNARIKYITAENFINEFVIHIRLDTMDELKEKFRNLDLLLIDDIQ
                     SLAKKTLSGTQEEFFNTFNALHNNNKQIVLTSDRTPDHLNDLEDRLVTRFKWGLTVNI
                     TPPDFETRVAILTNKIQEYNFIFPQDTIEYLAGQFDSNVRDLEGALKDISLVANFKQI
                     DTITVDIAAEAIRARKQDGPKMTVIPIEEIQAQVGKFYGVTVKEIKATKRTQNIVLAR
                     QVAMFLAREMTDNSLPKIGKEFGGRDHSTVLHAYNKIKNMISQDESLRIEIETIKNKI
                     K"
     gene            3897..5033
                     /gene="dnaN"
                     /locus_tag="VC122_001251"
     CDS             3897..5033
                     /gene="dnaN"
                     /locus_tag="VC122_001251"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002262647.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit beta"
                     /protein_id="HEP5641175.1"
                     /translation="MIQFSINRTLFIHALNATKRAISTKNAIPILSSIKIEVTSTGVT
                     LTGSNGQISIENTIPVSNENAGLLITSPGAILLEASFFINIISSLPDISINVKEIEQH
                     QVVLTSGKSEITLKGKDVDQYPRLQEVSTENPLILKTKLLKSIIAETAFAASLQESRP
                     ILTGVHIVLSNHKDFKAVATDSHRMSQRLITLDNTSADFDVVIPSKSLREFSAVFTDD
                     IETVEVFFSPSQILFRSEHISFYTRLLEGNYPDTDRLLMTEFETEVVFNTQSLRHAME
                     RAFLISNATQNGTVKLEITQNHISAHVNSPEVGKVNEDLDIVSQSGSDLTISFNPTYL
                     IESLKAIKSETVKIHFLSPVRPFTLTPGDEEESFIQLITPVRTN"
     gene            5108..5305
                     /locus_tag="VC122_001252"
     CDS             5108..5305
                     /locus_tag="VC122_001252"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003048966.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF951 family protein"
                     /protein_id="HEP5641176.1"
                     /translation="MYQIGSFVEMKKPHACVIKETGKKANQWKVLRVGADIKIQCTNC
                     QHVIMMSRYDFERKLKKVLQP"
     gene            5635..6750
                     /gene="ychF"
                     /locus_tag="VC122_001253"
     CDS             5635..6750
                     /gene="ychF"
                     /locus_tag="VC122_001253"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002269933.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="redox-regulated ATPase YchF"
                     /protein_id="HEP5641177.1"
                     /translation="MALTAGIVGLPNVGKSTLFNAITKAGAEAANYPFATIDPNVGMV
                     EVPDERLQKLTELITPKKTVPTTFEFTDIAGIVKGASRGEGLGNKFLANIREVDAIVH
                     VVRAFDDENVMREQGREDAFVDPIADIDTINLELILADLESINKRYARVEKMARTQKD
                     KESVAEFNVLQKIKPVLEDGKSARTIEFTEDEAKVVKGLFLLTTKPVLYVANVDEDKV
                     ANPDGIDYVKQIRDFAATENAEVVVISARAEEEISELDDEDKEEFLEAIGLTESGVDK
                     LTRAAYHLLGLGTYFTAGEKEVRAWTFKRGIKAPQAAGIIHSDFERGFIRAVTMSYDD
                     LMTYGSEKAVKEAGRLREEGKEYVVQDGDIMEFRFNV"
     gene            6820..7389
                     /locus_tag="VC122_001254"
     CDS             6820..7389
                     /locus_tag="VC122_001254"
                     /EC_number="3.1.1.29"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012657576.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminoacyl-tRNA hydrolase"
                     /protein_id="HEP5641178.1"
                     /translation="MVKMIVGLGNPGSKYEKTKHNIGFMAIDNIVKNLDVTFTDDKNF
                     KAQIGSTFINHEKVYFVKPTTFMNNSGIAVKALLTYYNIDITDLIVIYDDLDMEVSKL
                     RLRSKGSAGGHNGIKSIIAHIGTQEFNRIKVGIGRPLKGMTVINHVMGQFNTEDNIAI
                     SLTLDRVVNAVKFYLQENDFEKTMQKFNG"
     gene            7392..10895
                     /gene="mfd"
                     /locus_tag="VC122_001255"
     CDS             7392..10895
                     /gene="mfd"
                     /locus_tag="VC122_001255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010921764.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription-repair coupling factor"
                     /protein_id="HEP5641179.1"
                     /translation="MNILELFSQNKKVQSWHSGLTTLGRQLVMGLSGSSKALAIASAY
                     LDDQKKIVVVTSTQNEVEKLASDLSSLLDEELVFQFFADDVAAAEFIFASMDKALSRI
                     ETLQFLGNPKSQGVLIVSLSGLRTLLPNPDVFTKSQIQLTVGEDYDSDTLTKQLMTIG
                     YQKVSQVISPGEFSRRGDILDIYEITQELPYRLEFFGDDIDSIRQFHPETQKSFEQLE
                     GVFINPASDLIFEASDFQRGIEQLEKALQTAQDDKKSYLEDVLAVSKNGFKHKDIRKF
                     QSLFYEKEWSLLDYIPKGTPIFFDDFQKLVDKNARFDLEIANLLTEDLQQGKALSNLN
                     YFADNYRELRHYKPATFFSNFHKGLGNIKFDQMHQLTQYAMQEFFNQFPLLIDEIKRY
                     QKNQTTVIVQVESQYAYKRLEKSFQDYQFRLPLVSANQIVSRESQIVIGAISSGFYFA
                     DEKLALITEREIYHKKIKRRARRSNISNAERLKDYNELAVGDYVVHNVHGIGRFLGIE
                     TIQIQGIHRDYVTIQYQNSDRISLPIDQIGSLSKYVSADGKEPKINKLNDGRFQKTKQ
                     KVARQVEDIADDLLKLYAERSQQKGFSFSPDDDLQRAFDDDFAFVETEDQLRSIKEIK
                     ADMESMQPMDRLLVGDVGFGKTEVAMRAAFKAVNDHKQVAVLVPTTVLAQQHYENFKA
                     RFENYPVEVDVLSRFRSKKEQAETLERVRKGQIDIIIGTHRLLSKDVVFSDLGLIVID
                     EEQRFGVKHKETLKELKTKVDVLTLTATPIPRTLHMSMLGIRDLSVIETPPTNRYPVQ
                     TYVLENNPGLVREAIIREMDRGGQIFYVYNKVDTIEKKVSELQELVPEASIGFVHGQM
                     SEIQLENTLIDFINGDYDVLVATTIIETGVDISNVNTLFIENADHMGLSTLYQLRGRV
                     GRSNRIAYAYLMYRPDKVLTEVSEKRLEAIKGFTELGSGFKIAMRDLSIRGAGNILGA
                     SQSGFIDSVGFEMYSQLLEQAIASKQGKTTVRQKGNTEINLQIDAYLPDDYISDERQK
                     IDIYKRIREIQSREDYLNLQDELIDRFGEYPDQVAYLLEIALLKHYMDNAFAELVERK
                     NNQVIVRFEVTSLTYFLTQDYFEALSKTHLKAKISEHQGKIDIVFDVRHQKDYRILEE
                     LMLFGERLSEIKIRKNNSVFK"
     gene            11057..11329
                     /locus_tag="VC122_001256"
     CDS             11057..11329
                     /locus_tag="VC122_001256"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001234967.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA-binding S4 domain-containing protein"
                     /protein_id="HEP5641180.1"
                     /translation="MRLDKYLKVSRLIKRRSVAKEVADKGRIKVNGILAKSSTNIKLN
                     DHIEISFGNKLLTVRVIEIKDSTKKEDALKMYEIISETRITLNEEA"
     gene            11316..11687
                     /locus_tag="VC122_001257"
     CDS             11316..11687
                     /locus_tag="VC122_001257"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002987674.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="septum formation initiator family protein"
                     /protein_id="HEP5641181.1"
                     /translation="MKKPSIVQLNNHYIKKENLKKKFEEEESQKRNRFMGWILVSMMF
                     LFILPTYNLVKSYVDFEKQNQQVVKLKKEYNELSKRTKKEKQLAERLKDDNFVKKYAR
                     AKYYLSREGEMIYPIPGLLPK"
     gene            11822..>13102
                     /locus_tag="VC122_001258"
     CDS             11822..>13102
                     /locus_tag="VC122_001258"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011528130.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class A beta-lactamase-related serine hydrolase"
                     /protein_id="HEP5641182.1"
                     /translation="MRKLLAAMLMTFFLTPLPVISTEKKLIFSKNAVYQLKQDVVQST
                     QFYNQLPSNPNLYQETCAYKDSDLTLPAGRLGVNQPLLIKSLVLNKESLPVFELADGT
                     YVEANRQLIYDDIVLNQVDIDSYFWTQKKLRLYSAPYVLGTQTIPSSFSFAQKVHATQ
                     MAQTNHGTYYLIDDKGWASQEDLVQFDNRMLKVQEMLLQKYNNPNYSIFVKQLNTQTS
                     AGINADKKMYAASISKLAPLYIVQKQLQKKKLAENKTLTYTKDVNHFYGDYDPLGSGK
                     ISKIADNKDYRVEDLLKAVAQQSDNVATNILGYYLCHQYDKAFRSEIKALSGIDWDME
                     QRLLTSRSAANMMEAIYHQKGQIISYLSNTEFDQQRITKNITVPVAHKIGDAYDYKHD
                     VAIVYGNTPFILSIFTNKSTYEDITAIADDVYGIL"
BASE COUNT         4619 a         2049 c         2360 g         4074 t
ORIGIN      
        1 ttatttttaa ggtataatta agcaaagttc tataagctat ggacaaataa ttcggaggac
       61 aaaacatgcc aagtatgaaa tatatcttaa aatccttaag tattttacta attggatttt
      121 taggaggatt aatagcaatt attacattca ataatctcta cccacattct ccttcaaaaa
      181 taaattcagg taaggcaaca actagtaata tggtttttaa caacacaacc aatactacta
      241 aagctgtcaa agctgttcaa aatgcagttg tatcagttat taattatcaa gataaccctt
      301 cttcatccct ttctaaccct tatacaaaac tctttggaga agggcgttca aaagagaata
      361 aggatgctga attatctatt tttagtgaag gatctggggt catttatcga aaagatggca
      421 actccgctta cgttgttact aataaccatg ttatcgacgg agctaaacga attgaaattc
      481 ttatggcaga cggatctaaa gttgttggtg aattagttgg agctgatact tattcggatt
      541 tagctgttgt taagatctct tcagataaga taaaaacagt agctgaattt gcagactcta
      601 caaaactaaa tgttggagaa gttgctattg ctatcggcag cccactagga acacaatacg
      661 ctaattctgt tactcaagga atcgtctcta gtttaagtcg tactgtaact ttaaaaaacg
      721 agaatggtga gactgtctca acaaatgcta ttcagacaga tgctgctatt aaccctggaa
      781 actctggtgg accactaatt aatattgagg ggcaagtaat cggaattaat tcaagtaaaa
      841 tttcttctac cccaactggt agcaacggta atagtggtgc tgttgaagga attggttttg
      901 ctatcccatc tactgacgtt attaaaatta ttaaacaact agaaactaat ggtgaagtta
      961 tcagacctgc tcttggaata tccatggtca atttaaatga cttatccaca aatgctctta
     1021 gccaaattaa tattccaact agtgtaactg gtggtattgt agtagcagaa gttaaggaag
     1081 gaatgccggc atctggcaaa cttgctcagt acgatgtgat tacagaaatt gatggcaaaa
     1141 cagttaattc aattagtgat ttacaaagta gtctatacgg acacgatatt aatgatacta
     1201 ttaaagtaac tttttataga ggaactacaa agaaaaaagc agatatcaaa ttaacaaaaa
     1261 ctactcaaga tttgactaaa acgcagtaaa caagctacct aaatactatt acattttatc
     1321 acataaaaca gtccaaagga ctgttttatg tttcttattc tatccaggaa ctcccttatg
     1381 acaaaagaat tattgataga cctaccaatt gaagatattg ttacaaatcc ctatcaacca
     1441 aggatacaat ttaatcaaag agaactccaa gacctagcaa cttctattaa atcaaacggc
     1501 ctcattcaac ctattattgt tcgaaaatct gacatttttg gctatgagtt agttgctgga
     1561 gaacgacgac ttaaagcttc aaaaatggct ggtctaaaaa aagttccagc tatcatcaag
     1621 aagatctcta cactcgagag tatgcaacaa gctatagttg aaaatttaca acgttctaac
     1681 cttaacgcta tcgaagaagc taaagcctat cagttattgg ttgaaaaaaa acacatgact
     1741 cacgatgaga ttgctaaata tatgggaaaa tcaagacctt atattagcaa taccttacgt
     1801 ctgttacaac tcccagcacc tatcattaaa gcaattgaag aaggaaaaat tagtgcggga
     1861 cacgcgcgtg ctcttttaac tttgagtgat gataagcaac aactgtacct cactcataaa
     1921 atacaaaatg aaggcctaag tgttaggcaa attgagcaac tggttacttc tactccaagt
     1981 tcgaagctat ctaaaaaaac taaaaatatt tttgccactt ctttagagaa acaattggct
     2041 aaatcattgg gactctctgt caatatgaag ctgacagcaa accatagtgg gtaccttcag
     2101 atatcttttt ccaatgatga tgaattaaac agaattatca acaagctact ttagcttgtt
     2161 gatattctgt tttttctttt ttagttttcc acatgaaaaa tagttgaaaa caatagcggt
     2221 gtcacattaa aatggctttt ccacaggttg tggagaaccc aaattaacag tgttaattta
     2281 ttttccacag gttgtggaaa aactaactat tatccatcgt tctgtggaaa actagaatag
     2341 tttatggtag aatagtgcta gaattatcca caagaaggaa cctagtatga ctgaaaatga
     2401 acaaattttt tggaacaggg tcttggaatt agctcagagt caattaaaac aggcaactta
     2461 tgaatttttt gttcatgatg cccgtctatt aaaggtcgat aagcatattg caactattta
     2521 cttagatcaa atgaaagaac tcttttggga aaaaaatctt aaagatgtta ttcttactgc
     2581 tggttttgaa gtttataacg ctcaaatttc tgttgactat gttttcgaag aagacctaat
     2641 gattgagcaa aatcagacca aaatcaatca aaaacctaag cagcaagcct taaattcttt
     2701 gcctactgtt acttcagatt taaactcgaa atatagtttt gaaaacttta ttcaaggaga
     2761 tgaaaatcgt tgggctgttg ctgcttcaat agcagtagct aatactcctg gaactaccta
     2821 taatcctttg tttatttggg gtggccctgg gcttgggaaa acccatttat taaatgctat
     2881 tggtaattct gtactattag aaaatccaaa tgctcgaatt aaatatatca cagctgaaaa
     2941 ctttattaat gagtttgtta tccatattcg ccttgatacc atggatgaat tgaaagaaaa
     3001 atttcgtaat ttagatttac tccttattga tgatatccaa tctttagcta aaaaaacgct
     3061 ctctggaaca caagaagagt tctttaatac ttttaatgca cttcataata ataacaaaca
     3121 aattgtccta acaagtgacc gtacaccaga tcatctcaat gatttagaag atcgattagt
     3181 tactcgtttt aaatggggat taacagtcaa tatcacacct cctgattttg aaacacgagt
     3241 ggctattttg acaaataaaa ttcaagaata taactttatt tttcctcaag ataccattga
     3301 gtatttggct ggtcaatttg attctaatgt cagagattta gaaggtgcct taaaagatat
     3361 tagtctggtt gctaatttca aacaaattga cacgattact gttgacattg ctgccgaagc
     3421 tattcgcgcc agaaagcaag atggacctaa aatgacagtt attcccatcg aagaaattca
     3481 agcgcaagtt ggaaaatttt acggtgttac cgtcaaagaa attaaagcta ctaaacgaac
     3541 acaaaatatt gttttagcaa gacaagtagc tatgttttta gcacgtgaaa tgacagataa
     3601 cagtcttcct aaaattggaa aagaatttgg tggcagagac cattcaacag tactccatgc
     3661 ctataataaa atcaaaaaca tgatcagcca ggacgaaagc cttaggatcg aaattgaaac
     3721 cataaaaaac aaaattaaat aacatgtgga aaagaatatc ttttatgaaa tagttatcca
     3781 caagttgtga acaaccattt agtcttggat tctctcgttt atttagagtt atccactata
     3841 tacacaagac ctactactac tacttattat tatacttatt aaataaagga gttctcatga
     3901 ttcaattttc aattaatcgc acattattta ttcatgcttt aaatgcaact aaacgtgcta
     3961 ttagcactaa aaatgccatt cctattcttt catcaataaa aattgaagtc acttctacag
     4021 gagtaacttt aacagggtct aacggtcaaa tatcaattga aaacactatt cctgtaagta
     4081 atgaaaatgc tggtttgcta attacctctc caggagctat tttattagaa gctagttttt
     4141 ttattaatat tatttcaagt ttgccagata ttagtataaa tgttaaagaa attgaacaac
     4201 accaagttgt tttaaccagt ggtaaatcag agattacctt aaaaggaaaa gatgttgacc
     4261 agtatcctcg tctacaagaa gtatcaacag aaaatccttt gattttaaaa acaaaattat
     4321 tgaagtctat tattgctgaa acagcttttg cagccagttt acaagaaagt cgtcctattt
     4381 taacaggagt tcatattgta ttaagcaatc ataaagattt taaagcagta gcgactgact
     4441 ctcatcgtat gagccaacgt ttaatcactt tggacaatac ttcagcagat tttgatgtgg
     4501 ttattccaag taaatctttg agagaatttt cagcagtatt tacagatgat attgagaccg
     4561 ttgaggtatt tttctcacca agccaaatct tgttcagaag tgaacacatt tctttttata
     4621 cacgcctctt agaaggaaat tatcccgata cagatcgttt attaatgaca gaatttgaga
     4681 cggaggttgt tttcaatacc caatcccttc gccacgctat ggaacgtgcc ttcttgattt
     4741 ctaatgctac tcaaaatggt actgttaagc ttgagattac tcaaaatcat atttcagctc
     4801 atgttaactc acctgaggtt ggtaaggtaa acgaggattt agatattgtt agtcagtctg
     4861 gtagtgattt aactatcagc ttcaatccaa cttaccttat tgagtcttta aaagctatta
     4921 aaagtgaaac agtaaaaatt catttcttat caccagttcg accattcacc ctaacaccag
     4981 gcgatgagga agaaagtttt atccaattaa ttacaccagt acgaacaaac taagtaagaa
     5041 aaagctccct tttaggagtt tttttgttat tataaatatt aacgataata taagtggaga
     5101 aaagggaatg tatcaaattg gatcatttgt tgaaatgaaa aaacctcatg cttgtgtaat
     5161 taaagaaact ggtaaaaagg ctaatcaatg gaaggtactg agagtaggag ctgatattaa
     5221 aattcagtgt actaactgtc agcatgtgat tatgatgagt cgttatgatt ttgagcgaaa
     5281 attaaaaaaa gtactgcaac catgaaagtc cttttaaata aataattcta gctatatttg
     5341 caaccaatac tttcctaaaa aattgctagt atgccgttgg aaaattagct attctaatgt
     5401 tatcgaaaga agaaagatgg ccattgacaa tgacgaaagt tgtagcacag ttacttagtt
     5461 tctcggcaaa ctatatcaaa atgagaataa tacaccaaac tttgataatt taaatgaagg
     5521 tgaaagagtg gcataatgat aagcaaatct gaaattttct tcttattcat accgcttttt
     5581 cttctatttt ttggtataat aatcttgatt gaaatttgaa tggagattcg ctaaatggct
     5641 ttaacagcag gtattgtagg cttaccaaat gttggtaaat caaccttatt taacgcaatt
     5701 acaaaagcag gagcagaagc tgctaattat ccttttgcga ctattgatcc caatgttggt
     5761 atggtagagg ttccagatga gcgtctgcaa aaattgacag aattgattac acctaaaaaa
     5821 acagttccga caacctttga atttactgat attgcaggta tcgttaaagg tgcttctaga
     5881 ggggaaggtc taggaaataa atttttagct aatattcgtg aagtcgatgc tattgttcat
     5941 gtggtacgtg cttttgatga tgaaaatgtc atgcgtgaac aaggtcgtga ggatgctttc
     6001 gttgatccta tagcagatat tgacactatt aatcttgaat taatcttagc tgatttagag
     6061 tcaatcaata aacgttatgc gcgtgttgaa aaaatggcac gaactcaaaa agataaagaa
     6121 tcagtagcag agtttaatgt tttgcaaaag attaaacctg ttttggaaga tgggaaatca
     6181 gctaggacaa ttgagtttac agaagatgaa gcaaaagttg ttaaaggtct ctttttatta
     6241 acaactaaac ctgttttata tgtagctaat gtcgatgaag ataaagttgc taatccagat
     6301 ggtattgatt atgtcaaaca aattcgtgac tttgcagcta ctgaaaatgc tgaagtagtt
     6361 gttatctcag cgcgtgcaga agaagaaatt tcagagcttg acgatgagga taaagaagaa
     6421 tttttggaag ctatcggtct tactgaatca ggcgttgata aattaaccag agcagcttat
     6481 catctcttag gccttggaac ctattttaca gcaggtgaaa aagaggttcg tgcttggacg
     6541 tttaagcgtg gtataaaagc tccacaagct gctggtatta tccattcaga ttttgaaaga
     6601 ggttttattc gtgcagtaac catgtcttat gatgatctaa tgacctacgg ttcagaaaaa
     6661 gccgtcaaag aagctggacg cttgcgtgaa gaaggaaaag aatatgttgt ccaagatggg
     6721 gacatcatgg aattcagatt taatgtgtaa ttatattaaa acaatatcag aaggttggaa
     6781 gaacattcca gcccttttgg cattttagaa agagaaaata tggtaaaaat gattgttggt
     6841 ctgggaaatc caggctctaa atatgaaaaa acaaagcaca atattggttt tatggctatt
     6901 gacaatattg tcaagaacct tgacgttacc tttacagatg ataaaaattt taaagcacag
     6961 ataggaagta cttttattaa tcatgaaaaa gtttactttg tgaaacctac tacttttatg
     7021 aataatagcg gcatagcagt aaaagcatta ctaacctact ataatattga cataacagat
     7081 ttaattgtta tctatgatga tttagacatg gaagtcagta aattacgttt acgtagtaag
     7141 ggttcagcag gaggacataa tggcattaag tcaatcattg cccacattgg aactcaggaa
     7201 tttaaccgaa tcaaagttgg tattggacga cctttaaaag gtatgactgt tattaaccat
     7261 gtgatgggcc aattcaatac cgaagataat attgctattt cgttaactct tgacagagtt
     7321 gtcaatgctg tcaagtttta tttacaagaa aatgattttg aaaaaacaat gcagaaattt
     7381 aatggataat catgaatatt ttagaattat ttagtcagaa taagaaagtc caatcctggc
     7441 actctggatt aaccacctta ggaagacaac tggtaatggg gttatcgggt tcaagtaaag
     7501 cattggctat agcttccgct tatttagatg atcaaaaaaa aatagttgtg gttacatcaa
     7561 ctcaaaatga ggttgaaaaa ttagccagcg atttatctag tttacttgat gaagaacttg
     7621 ttttccaatt ttttgcagac gatgtggctg cagcggaatt tatctttgcg tcaatggata
     7681 aagctctatc aagaatagaa accctgcaat ttttagggaa tcctaaatct cagggcgttt
     7741 taattgttag tttatcaggc ttaagaactt tattgccaaa cccagatgtt tttacaaaga
     7801 gtcagattca actaacagtt ggagaagatt atgatagtga tactcttact aaacaactga
     7861 tgacaattgg ctatcagaag gtctcacagg tcattagtcc gggagaattt agccgtcgag
     7921 gggatatttt agatatctat gagattacac aagaattgcc ttatcgattg gaattttttg
     7981 gcgatgatat tgatagtatt aggcaatttc atccagaaac tcaaaaatct tttgaacaac
     8041 tagaaggtgt ttttattaat ccagcaagtg atcttatttt tgaggctagt gattttcaac
     8101 gtggcattga gcaattagaa aaggctctac aaacagcaca agatgataaa aaatcttatt
     8161 tagaagatgt attagctgtt tcaaaaaacg gttttaaaca taaggatatc cgtaaatttc
     8221 aatcattatt ttacgaaaaa gagtggtcat tattagatta tattcctaag ggaacgccaa
     8281 tcttttttga tgattttcaa aaactagttg ataaaaatgc aagatttgat ttagagattg
     8341 ctaatctctt gacagaagat ttacagcaag gaaaggctct ttccaatctt aactactttg
     8401 cagataatta tcgagagctt aggcactata agccagcgac cttcttttca aattttcata
     8461 agggacttgg aaatatcaaa tttgatcaga tgcatcagct aactcagtat gccatgcagg
     8521 aattttttaa tcaatttcct ttgttgattg atgagattaa acggtatcaa aaaaatcaaa
     8581 caacggttat tgtacaggta gagtctcagt atgcttacaa acgacttgaa aaatcttttc
     8641 aagattacca atttcgcctt cctttagtga gtgctaatca aattgtttca cgtgaatcac
     8701 aaattgtaat tggagctatc tccagtggtt tttattttgc tgatgaaaag ttagcgttaa
     8761 tcacagagcg tgaaatttat cataaaaaga tcaaacgacg cgctagacga tctaatatta
     8821 gcaatgctga gcgtttgaaa gattacaatg agttagcagt aggtgattac gtggttcata
     8881 atgtccatgg tatcggtcgc tttcttggaa ttgaaacgat tcagattcag ggaatccatc
     8941 gagattatgt tactattcaa tatcaaaatt cagaccgtat ttctcttcca attgaccaaa
     9001 ttggtagctt atcaaagtac gtttctgctg atggaaaaga acctaaaatt aataaactca
     9061 atgatggtcg tttccaaaag acaaagcaaa aggtcgctag acaagtagag gatattgctg
     9121 atgaccttct aaaattatat gctgaaagaa gtcagcaaaa aggattttca ttttcaccag
     9181 atgatgactt gcagcgcgct tttgatgatg attttgcttt tgtagaaaca gaagatcaac
     9241 ttaggtctat aaaggaaatt aaagctgata tggagagcat gcagcctatg gatcgtcttt
     9301 tagtaggcga tgtaggattt ggtaagacag aagtagctat gagggcagcc tttaaagcgg
     9361 tgaacgatca caaacaagta gctgtcttag ttccaaccac agtcttggcc cagcagcatt
     9421 atgaaaactt caaagcacgc tttgaaaatt accctgttga ggttgacgtc ttaagtcgtt
     9481 tccgtagtaa aaaagagcaa gctgaaacac tagaacgtgt acgaaaaggt caaattgaca
     9541 ttattatcgg aacccatcga ctcctatcaa aagatgtggt cttttctgat ttaggattga
     9601 ttgtgattga tgaggaacaa cggtttggtg ttaagcataa agaaacttta aaggaattaa
     9661 aaactaaggt tgacgtctta accttaacag ctactccgat tcctagaacc ttacacatgt
     9721 ctatgttagg tatccgagat ttatcggtta ttgagacccc accaaccaat cgttatcctg
     9781 ttcaaaccta tgttttggaa aataatccag gtctcgttag agaagctatc attcgtgaaa
     9841 tggatcgtgg aggacaaatt ttttacgttt acaataaagt tgacactatt gaaaagaaag
     9901 tttcagagct acaagaatta gtcccagaag cttctatagg ttttgttcat gggcaaatga
     9961 gtgaaattca acttgaaaat accttgattg actttataaa tggtgattat gatgtccttg
    10021 tggctacaac aatcatcgaa acaggagttg acatttctaa tgtaaacact ttgtttattg
    10081 agaatgctga tcatatggga ttgtcaactt tatatcaact acgagggcgc gtcggaagaa
    10141 gtaatcgtat tgcttatgct tacctgatgt atcgtcctga taaggtccta acagaggttt
    10201 ctgaaaagcg tttagaggct attaaaggct ttactgaatt aggttcaggc ttcaagattg
    10261 ctatgcgaga tttgtctata cgaggagcag gtaatatttt aggggcttct cagagtggct
    10321 ttattgattc ggtcggtttt gaaatgtatt ctcagttatt ggaacaggct attgctagca
    10381 agcaaggaaa aacgactgtt cgccaaaaag gtaatactga aatcaatctt cagattgacg
    10441 cctatttgcc agatgattac atatcagatg agcgtcaaaa aattgacatt tacaagcgta
    10501 ttcgagaaat tcaatcaaga gaagattatc tcaatttgca ggatgagctg atagatcgtt
    10561 ttggagagta tcctgatcaa gttgcctatt tgttagagat agctctgcta aaacattata
    10621 tggacaatgc ctttgcggaa ttagttgaac gcaaaaataa tcaggtcatt gttcgatttg
    10681 aagtaacttc tttaacttat ttcttgactc aggattattt cgaagcctta tcgaaaaccc
    10741 atcttaaagc taaaattagt gaacatcagg ggaaaatcga tatcgtcttt gacgttcgcc
    10801 atcaaaaaga ttatagaatt ttagaagaat tgatgttatt tggagaaagg cttagtgaga
    10861 taaaaatcag aaaaaacaat tcagttttta aataataatt tgaaacaact tttagactga
    10921 atagtattaa tgacaatgga acgaactagc tctttatact tttgtcaaaa atctaagatc
    10981 tttgctgcga aattaggatc ttttctacca tttaaaaaat aaaaatgata aaatgaagag
    11041 gattgtagga gaaattatga gactagataa atatctaaag gtatcgcgcc ttattaaacg
    11101 tcgttcagta gcaaaagaag ttgcggataa gggacgaatt aaagttaatg ggatacttgc
    11161 taaaagttca acgaatataa aactaaatga tcacattgaa attagttttg gaaataaatt
    11221 actgacagta agagtcattg aaataaaaga tagtacaaaa aaagaagatg ctcttaagat
    11281 gtatgagata atcagtgaaa caaggataac attaaatgaa gaagcctagt attgttcaat
    11341 taaataatca ttatattaag aaagagaatc tcaaaaaaaa atttgaagaa gaagaatctc
    11401 aaaaaagaaa tcgttttatg ggatggatcc ttgtaagtat gatgttttta tttattttgc
    11461 caacttataa tcttgtcaaa agttatgttg attttgaaaa gcaaaatcaa caggtggtta
    11521 aattaaaaaa agagtataat gaattgtcaa agaggacaaa aaaagaaaaa caattagcag
    11581 aacgactaaa agatgataat tttgtcaaaa aatatgctag ggcaaaatac tatttatcgc
    11641 gtgaaggaga aatgatttat cctattccag gactattacc aaaatgatga tggataatat
    11701 tgtaaaaaaa atagaagcat ttcttgcttt ttctgataaa aaattagcag agctgcaaca
    11761 ggaaaatcaa aaagttaaag aagaaagtca gtatattcaa aaaaactaga aaggagtcaa
    11821 tatgagaaaa ttattagcgg ctatgttaat gacttttttt ctgactcctt taccagtgat
    11881 tagtacagaa aaaaaactta tattttcaaa aaatgctgtt tatcaattga aacaagatgt
    11941 cgttcaatca acacaattct ataatcagct accctctaat ccaaatcttt atcaagaaac
    12001 gtgtgcctat aaagacagtg atttaactct accagcagga agattaggtg taaatcaacc
    12061 attacttatt aaatcgcttg ttcttaacaa agaatcttta ccggtttttg agttagctga
    12121 tggtacctat gttgaggcta atcgacaatt gatttatgac gatattgtac ttaatcaagt
    12181 agatatagat agctattttt ggacacaaaa gaaacttagg ctttattcag ccccttatgt
    12241 tttaggtacg caaacaattc cttcttcttt ttcatttgct caaaaagttc atgccactca
    12301 aatggcacaa acaaaccatg gaacttatta tcttattgat gataagggct gggcatcaca
    12361 agaagatcta gttcaatttg ataaccgcat gttaaaagtc caggaaatgc tcttacaaaa
    12421 atataataac ccaaattatt caatttttgt aaagcaactc aacacacaaa caagtgctgg
    12481 tattaatgct gataaaaaaa tgtatgctgc aagtatctcg aagttagcac cactttatat
    12541 tgttcaaaaa caattacaaa aaaagaaatt agcagagaat aaaactttga cttatactaa
    12601 agatgttaat catttttatg gagactatga tccattggga agtggtaaaa ttagtaaaat
    12661 agctgataat aaagattatc gtgttgaaga cctactgaaa gctgtagcac aacaatcgga
    12721 taatgtagca actaatattt taggttatta tctatgtcat cagtatgata aagctttccg
    12781 ctcagagata aaagctttat caggtatcga ttgggatatg gagcagcgct tattaacttc
    12841 tcgttcagct gcaaatatga tggaagctat ttatcatcaa aaaggccaaa ttatttctta
    12901 cctttcaaat accgaatttg atcaacaacg tatcacaaaa aatattactg ttccagttgc
    12961 acataaaatt ggtgatgctt atgattataa acatgacgtt gctattgttt acggtaatac
    13021 tccatttatt ttgtctattt ttacaaataa atcaacctat gaagatatta cggctattgc
    13081 agatgacgtt tatggtattt ta
//
LOCUS       DATXIG010000041        12439 bp    DNA     linear   BCT 28-DEC-2023
DEFINITION  TPA_asm: Streptococcus pyogenes strain MGAS11113
            SAMN08555799-rid19682583.denovo.041, whole genome shotgun sequence.
ACCESSION   DATXIG010000041 DATXIG010000000
VERSION     DATXIG010000041.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN08555799
            Sequence Read Archive: SRR6740495
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pyogenes
  ORGANISM  Streptococcus pyogenes
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 12439)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12439)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-OCT-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-OCT-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001918058.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001918058.2
            Genome Coverage       :: 368x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/12/2023 11:23:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 1,806
            CDSs (total)                      :: 1,769
            Genes (coding)                    :: 1,722
            CDSs (with protein)               :: 1,722
            Genes (RNA)                       :: 37
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1, 1 (5S, 23S)
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 47
            CDSs (without protein)            :: 47
            Pseudo Genes (ambiguous residues) :: 0 of 47
            Pseudo Genes (frameshifted)       :: 30 of 47
            Pseudo Genes (incomplete)         :: 20 of 47
            Pseudo Genes (internal stop)      :: 9 of 47
            Pseudo Genes (multiple problems)  :: 11 of 47
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12439
                     /organism="Streptococcus pyogenes"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN08555799-rid19682583.denovo.041"
                     /strain="MGAS11113"
                     /serotype="Emm28"
                     /isolation_source="normally sterile site"
                     /host="Homo sapiens"
                     /db_xref="taxon:1314"
                     /country="Finland"
                     /collection_date="2002"
                     /collected_by="National Public Health Laboratory"
     gene            48..1391
                     /locus_tag="VC122_001259"
     CDS             48..1391
                     /locus_tag="VC122_001259"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014635431.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Mur ligase family protein"
                     /protein_id="HEP5641183.1"
                     /translation="MKMKTLLGIIAGKAAQSILTKLGRGSTYPGRLALACNKDILKDL
                     SKDYDIVVVTGTNGKTLTTALTVGILKEAFGEIITNPSGANMITGITSTFLAAKKGKS
                     GRQIAVLEIDEASLPRITTYLKPSLFVYTNIFRDQMDRYGEIYTTYQMIVDGARNAPK
                     ATILANGDSPIFSSKDIVNPVQYYGFDTAKHAPQLAHYNTEGILCPKCEHILQYRLNT
                     YANLGDFICLNCGFKRPQLDYRLTELTAITHQSSEFVIDGQNYKINVGGLYNIYNALA
                     AVSVAEFFGVSPEKIKAGFNKSKAVFGRQETFTIGDKSCTLILIKNPVGASQALEMIQ
                     LADYPFSLSVLLNANYADGIDTSWIWDANFELITQMPITEINAGGVRHSEIARRLRVT
                     GFDDTKIKQAEKLEQIMEAIEKQESKHAYILATYTAMLEFRSLLADRHVVEKEMN"
     gene            1391..2182
                     /locus_tag="VC122_001260"
     CDS             1391..2182
                     /locus_tag="VC122_001260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002984860.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type 1 glutamine amidotransferase"
                     /protein_id="HEP5641184.1"
                     /translation="MTYTSLKSPENTDYLYDLNIAHLYGNLMNTYGDNGNVLMLKYVA
                     EKLGARVTVDIVSINDTFEQDDYDIVFFGGGQDYEQSIVAKDLPSKKAALADYIANNK
                     VVLAICGGFQLLGQYYVQANGVKIDGLGIMGHYTLNQHQNRFIGDIKIHNDEFNETYY
                     GFENHQGRTFLSGDEKPLGRVVYGNGNNKEDQTEGVHYKNVYGSYFHGPILSRNVNLA
                     YRLVTTSLKKKYGSAISLPSYDDILKQEITEEYADLKSKASFNKV"
     gene            complement(2295..3284)
                     /locus_tag="VC122_001261"
     CDS             complement(2295..3284)
                     /locus_tag="VC122_001261"
                     /EC_number="6.3.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007893175.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoate--protein ligase"
                     /protein_id="HEP5641185.1"
                     /translation="MKYIVNKSHNPAFNIALEAYAFRELVEEDELFILWINEPAIIIG
                     KHQNTIQEINKEYIDEHGIHVVRRLSGGGAVYHDLNNLNYTIISNKTAEGAFDFKTFS
                     QPVIATLADLGVTANFTGRNDIEIDGKKICGNAQAYYKGRMMHHGCLLFDVDMTVLGD
                     ALKVSKDKIESKGVKSVRARVTNILNELPEKITVEEFSDKILTKMKETYPDMTEYVLS
                     EDELAKIEQSAKEQFGSWDWTYGKAPEYTIERNVRYPAGKISTFANVENSIIKNLKIY
                     GDFFGIKDVQDIENLLIGCKYEYRDVFECLKTIDTTQYFSRMTVEEVAKAIVS"
     gene            3517..5934
                     /gene="hylA"
                     /locus_tag="VC122_001262"
     CDS             3517..5934
                     /gene="hylA"
                     /locus_tag="VC122_001262"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010922241.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hyaluronidase"
                     /protein_id="HEP5641186.1"
                     /translation="MNTYFCTHHKQLLLYSNLFLSFAMMGQGTAIYADTLTSNSEPNN
                     TYFQTQMLTTTDSEKKVVQPQQKDYYTELLDQWNSIIAGNDAYDKTNPDMVTFHNKAE
                     KDAQNIIKSYQGPDHENRTYLWEHAKDYSASANITKTYRNIEKIAKQITNPESCYYQD
                     SKAIAIVKDGMAFMYEHAYNLDRENHQTTGKENKENWWVYEIGTPRAINNTLSLMYPY
                     FTQEEILKYTAPIEKFVPDPTRFRVRAANFSPFEANSGNLIDMGRVKLISGILRKDDL
                     EISDTIKAIEKVFTLVDEGNGFYQDGSLIDHVVTNAQSPLYKKGIAYTGAYGNVLIDG
                     LSQLIPIIQKTKSSIEADKMATIYHWINHSFFPIIVRGEMMDMTRGRSISRFNAQSHV
                     AGIEALRAILRIADMSEEPHRLALKTRIKTLVTQGNAFYNVYDNLKTYHDIKLMKELL
                     SDTSVPVQKLDSYVASFNSMDKLALYNNKHDFAFGLSMFSNRTQNYEAMNNENLHGWF
                     TSDGMFYLYNNDLGHYSENYWATVNPYRLPGTTETEQKPLEGTPENIKTNYQQVGMTS
                     LSDDAFVASKKLNNTSALAAMTFTNWNKSLTLNKGWFILGNKIIFVGSNIKNQSSHKA
                     YTTIEQRKENQKHPYHAYVNNQPVDLNNQLVDFTNTKSIFLESDDSAQNIGYYFFKPT
                     TLSISKALQTGKWQNIKADDKSPEAIKEVSNTFITIMQNHTQDGDRYAYMMLPNMTRQ
                     EFETYISKLDIDLLENNDKLAAVYDHDSQQMHVIHYEKKATMFSNHNLSHQGFYSFPH
                     PVKQNQQ"
     gene            complement(6607..8370)
                     /gene="lpdA"
                     /locus_tag="VC122_001263"
     CDS             complement(6607..8370)
                     /gene="lpdA"
                     /locus_tag="VC122_001263"
                     /EC_number="1.8.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002984864.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrolipoyl dehydrogenase"
                     /protein_id="HEP5641187.1"
                     /translation="MAVEIIMPKLGVDMQEGEIIEWKKQEGDTVNEGDILLEIMSDKT
                     NMELEAEDSGVLLKITRQAGETVPVTEVIGYIGAEGESVEVSSPAASDVNVARTTEDL
                     EAAGLEVPKAPAQAASAAPKAALADDEYDIIVVGGGPAGYYAAIRGAQLGGKIAIVEK
                     SEFGGTCLNVGCIPTKTYLKNAEILDGIKIAAGRGINLASTNYTIDMDKTVDFKNTVV
                     KTLTGGVQGLLKANKVTIFNGLGQVNPDKTVTIGSQTIKGRNVILATGSKVSRINIPG
                     IDSKLVLTSDDILDLREMPKSLAVMGGGVVGIELGLVWASYGVDVTVIEMADRIIPAM
                     DKEVSLELQKILSKKGMKIKTSVGVSEIVEANNQLTLKLNNGEEVVAEKALLSIGRVP
                     QMNGLENLNLEMDRNRIKVNDYQETSIPGIYAPGDVNGTKMLAHAAYRMGEVAAENAI
                     HGNTTRKANLKYTPAAVYTHPEVAMVGLTEEQAREQYGDVLIGKNSFTGNGRAIASNE
                     AHGFVKVIADAKYHEILGVHIIGPAAAEMINEAATIMESELTVDELLLSIHGHPTFSE
                     VMYEAFADVLGEAIHNPPKRK"
     gene            complement(8701..10110)
                     /locus_tag="VC122_001264"
     CDS             complement(8701..10110)
                     /locus_tag="VC122_001264"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011284735.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrolipoamide acetyltransferase"
                     /protein_id="HEP5641188.1"
                     /translation="MAFEIIMPKLGVDMQEGEIIEWKKQEGDTVNEGDILLEIMSDKT
                     NMELEAEDSGVLLKIVRPAGDTVPVTEVIGYIGAEGESVDTIASSEKTTEIPVPASAD
                     AGPAVAPKENVASPAPQVAATAIPQGNGGKVRATPAARKAAAEMGIDLGQVPGTGPKG
                     RVHKADVENFKGAQPKASPLARKIAADKGIDLATVSGTGFNGKVMKEDIMAILEAAKP
                     AEAKAPAAKEEKVVDLAEGVEHKPMSAMRKAISKGMTNSYLTAPTFTLNYDIDMTEMI
                     ALRKKLIDPIMAKTGLKVSFTDLIGMAVVKTLMKPEHEYMNASLINDANDIELHRFVN
                     LGIAVGLDDGLIVPVIHGADKMSLSDFVLASKDVIKKAQTGKLKAAEMSGSTFSITNL
                     GMFGTKTFNPIINQPNSAILGVGATIPTPTVVDGEIVARPIMAMCLTIDHRLVDGMNG
                     AKFMVDLKKLMENPFELLI"
     gene            complement(10295..11293)
                     /locus_tag="VC122_001265"
     CDS             complement(10295..11293)
                     /locus_tag="VC122_001265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012677772.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-ketoacid dehydrogenase subunit beta"
                     /protein_id="HEP5641189.1"
                     /translation="MSETKLMALREAVNLAMTEEMRKDENIFLMGEDVGVYGGDFGTS
                     VGMIEEFGPKRVKDTPISEAAISGAAIGAAITGLRPIVDVTFMDFLTIMMDAIVNNGA
                     KNNYMFGGGLITPVTFRVASGSGIGSAAQHSQSLEAWLTHIPGIKVVAPGNANDAKGL
                     LKSAIRDNNIVLFMEPKALYGKKEEVNQDPDFYIPLGKGDIKREGTDLTIVSYGRMLE
                     RVLQAAEEVAADGINVEVVDPRTLIPLDKELIIESVKKTGKLMLVNDAYKTGGFIGEI
                     ATMITESEAFDYLDHPIVRLASEDVPVPYARVLEQAILPDVEKIKAAIVKMANKGN"
     gene            complement(11352..12320)
                     /locus_tag="VC122_001266"
     CDS             complement(11352..12320)
                     /locus_tag="VC122_001266"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002989983.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine pyrophosphate-dependent dehydrogenase
                     E1 component subunit alpha"
                     /protein_id="HEP5641190.1"
                     /translation="MVTVSKEQHLDMFLKMERIREFDSRINKLVRRGFVQGMTHFSVG
                     EEAANVGAVAHLSYDDIIFSNHRGHGQSIAKDMDLNKMMAELAGKATGVSKGRGGSMH
                     LADFEKGNYGTNGIVGGGYALAVGAALTQQYKGTNNIAVAFSGDGATNEGSFHESVNM
                     AATWKLPVIFFIINNRYGISMSINNATNTPHLYTRAEAYGIPGFYCEDGNDVMAVYET
                     MGKAVEHVRGGNGPAIVEVESYRWFGHSTADAGKYRTKEEVDEWKEKDPMIKYRTYLT
                     SEGIATDDELDAIQAQVKKEVDDAYEFAQNSPDPELSVAFEDVWVD"
BASE COUNT         3780 a         2561 c         2235 g         3863 t
ORIGIN      
        1 agatttttcc ctatctaaaa tcaataaact tatgataaaa tataagcatg aaaatgaaga
       61 cattactcgg cattattgct ggcaaagctg ctcaatccat tttaacaaaa ctagggcgag
      121 gatctactta tcctggtcga ttggctctag cttgtaataa agatatttta aaggatttgt
      181 ctaaggacta tgatattgtg gtcgtcactg gtactaatgg taaaacgtta acaactgctc
      241 ttactgtcgg tattttgaaa gaggcatttg gcgagataat cacaaatcca agtggtgcca
      301 atatgattac tggaattact tctacttttt tagctgctaa aaaagggaaa tctggaaggc
      361 aaattgctgt tttagaaatt gacgaagcta gtcttcctag aattaccact tacttaaaac
      421 ctagtctttt tgtttatact aatattttta gggatcagat ggaccgctac ggagagattt
      481 atactactta tcagatgatt gtagatggtg ctcgaaatgc tccaaaagca actattctag
      541 ccaatggaga tagtcctatt ttctcttcta aggacatagt taatccagtt cagtattatg
      601 gttttgatac tgctaagcat gctccgcagc tagctcatta caacacagaa gggattttat
      661 gtccaaaatg cgaacatatt cttcagtatc gtctcaatac ttatgcgaat ctaggagact
      721 ttatttgttt aaattgtggc ttcaagagac cacagttgga ttatcgatta actgaactca
      781 ccgctattac tcaccaaagt tctgaatttg ttattgatgg acaaaactac aagataaatg
      841 ttggtggtct ttataatatt tacaatgcgc ttgctgcggt ttctgttgct gaatttttcg
      901 gtgtttctcc cgagaaaatc aaggcgggct ttaacaaaag taaggcggtt tttggaagac
      961 aagaaacttt tactattggt gataaatcat gcacccttat ccttatcaag aatcctgttg
     1021 gagccagtca agcgcttgaa atgattcagc tagctgatta cccatttagc ttatctgtgc
     1081 ttttaaatgc taattacgct gacggcattg ataccagctg gatttgggat gctaattttg
     1141 aactcattac ccaaatgccc attacagaaa ttaatgctgg cggcgtccgt cactctgaaa
     1201 ttgccagaag acttagagtc acaggatttg acgacactaa gataaaacaa gctgaaaagc
     1261 tcgagcaaat catggaagct attgaaaaac aagaaagcaa acatgcttat attttagcaa
     1321 cctatactgc aatgttagaa ttccgtagct tgctagccga ccgccatgtt gttgaaaagg
     1381 agatgaacta atgacttaca cttcactaaa atctccagaa aacactgact acctctatga
     1441 cctaaacatt gctcatctct acggaaacct gatgaatacc tacggtgaca atgggaatgt
     1501 cttgatgctc aagtatgtcg ctgaaaaact tggagctagg gttactgttg atattgtatc
     1561 tatcaatgat acctttgaac aagacgatta cgatattgtt ttctttggtg gtggtcaaga
     1621 ttatgaacaa agcattgttg ccaaggattt gcctagcaaa aaagctgcct tagcagatta
     1681 cattgctaat aataaagtgg tgcttgccat ctgtggtggc ttccaattac tagggcaata
     1741 ctatgtccaa gctaatgggg ttaaaattga tggacttgga attatgggac actacaccct
     1801 taatcagcac caaaatcgct ttattggaga tattaaaatt cataatgacg aatttaacga
     1861 aacctattat ggctttgaaa atcatcaagg acgtaccttc ttatctggtg acgaaaagcc
     1921 acttggacga gtggtttatg gcaacggtaa taataaagaa gaccaaactg aaggtgttca
     1981 ctataaaaac gtctatggta gttatttcca cggtcctatc ttatcacgca atgttaatct
     2041 tgcttaccgc ttagtaacaa catctcttaa aaaaaagtat ggatcagcta tttcactacc
     2101 tagctatgat gatatactga aacaagaaat cactgaagaa tatgccgacc ttaaaagcaa
     2161 agcttccttt aataaagttt aagttcttct ttcatgtcat caaacaaaaa ctcctaagca
     2221 ggacacgata gatagttcgc ttaggagttt tatagttttt tcttctgaca gagcttctta
     2281 agtgattaaa accactaaga aacaatagct ttagcaactt cttcaacggt catgcgtgag
     2341 aagtactggg tcgtatcaat cgtttttaaa cattcaaaaa catcacggta ctcatattta
     2401 caaccaatta ataagttctc aatgtcttgc acatctttga tcccaaagaa atcaccatag
     2461 attttaaggt ttttaatgat agaattttca acatttgcaa aggttgaaat tttacctgca
     2521 gggtaacgaa cattgcgttc aatcgtatat tctggcgctt tgccgtaggt ccaatcccaa
     2581 ctaccaaatt gctctttggc agattgttca atcttagcca actcatcctc tgacaagaca
     2641 tattctgtca tgtctggata cgtttctttc atcttggtca agattttatc tgaaaattcc
     2701 tcaacggtaa ttttttctgg taattcattt aaaatattgg tcacgcgagc acgaacagac
     2761 ttcactcctt tagactcaat cttatcctta cttaccttaa gagcatcgcc aagaacggtc
     2821 atatccacat caaacaaaag gcaaccatgg tgcatcatgc gacctttgta gtaagcttga
     2881 gcattaccac agattttttt gccatcaatt tcaatatcat tacggcctgt aaagttggct
     2941 gttacaccca aatcagctaa agtcgcaatc acaggttgtg agaaggtttt gaaatcaaat
     3001 gcgccttccg ctgttttatt tgaaatgata gtgtagttca agttattcaa atcatgataa
     3061 accgcaccac caccagataa acgtcgcaca acatgaatac catgttcatc aatgtattct
     3121 ttattgattt cttgaatggt gttttgatgt ttgccaataa taatagctgg ctcattgatc
     3181 caaaggataa acaattcgtc ttcttccact agttctctaa aagcataggc ttccaaagcg
     3241 atgttaaagg caggattgtg acttttgtta acgatgtatt tcataggaga aaaaatcctt
     3301 tcaaaaatgt aatctacctt tctagtataa tgaaaacaaa tataaaagac aagtgtccta
     3361 ttctgtatga catcttcagt acctacactt attagtgcac aaaaactcag ccattattta
     3421 tgtaagggtt tataaaaata ctttaacgtg gtatattttt acttagtaaa ccaatttact
     3481 tttattatct catttttata taggaggaat ttatcagtga atacttattt ttgcacacac
     3541 cataaacaat tactacttta ttcaaaccta ttccttagct ttgctatgat gggccaagga
     3601 actgccattt atgccgatac actgacttca aattcagaac ctaataatac ttactttcaa
     3661 acgcaaatgc tcactactac agatagcgaa aaaaaggtag tacagccaca acaaaaagac
     3721 tactatactg aattgttaga ccaatggaac agtattatcg caggcaacga tgcttatgat
     3781 aaaaccaatc ctgacatggt cacttttcat aataaagctg aaaaggatgc tcaaaacatt
     3841 attaaaagct atcaagggcc tgaccacgaa aatagaactt acctttggga acatgcaaag
     3901 gattattccg cttctgctaa tatcacgaaa acttaccgca atattgaaaa aatagcaaaa
     3961 cagatcacta atcctgagtc atgctattat caagatagta aagctattgc tattgtaaaa
     4021 gacggtatgg ccttcatgta tgaacacgct tataatctag atcgtgaaaa tcatcaaaca
     4081 actggaaaag aaaacaaaga aaattggtgg gtttatgaaa ttggaactcc tcgtgctatt
     4141 aataatacct tatccttgat gtatccttat tttactcaag aagaaatcct taaatacaca
     4201 gctccaatcg aaaaatttgt gcctgaccct actcgtttta gggttcgcgc tgccaatttt
     4261 tcaccttttg aagccaatag cggaaattta attgatatgg ggcgtgttaa actcatttcc
     4321 ggtattcttc gtaaagatga tctcgaaatt agtgatacaa tcaaagcaat tgagaaagtt
     4381 ttcacgctag ttgacgaagg aaatggtttt taccaagacg gttctttaat tgatcacgtg
     4441 gttactaacg ctcaaagtcc actttataaa aaaggcattg cttacactgg agcttacggt
     4501 aatgttctta tagatggctt atcgcaatta attcctatta ttcaaaaaac aaagtcttct
     4561 atagaagcgg ataaaatggc tactatctat cattggatta accattcttt tttccctatc
     4621 atcgttcgtg gagaaatgat ggatatgact cgagggcgtt ctatcagtcg ttttaatgct
     4681 caatctcatg ttgctggcat tgaagcactt cgtgctattt tacgtattgc tgacatgtct
     4741 gaagagcctc accgtttggc acttaaaaca cgtataaaaa cactcgtcac acaagggaat
     4801 gctttttaca atgtctatga taatttgaaa acctatcacg atatcaaact tatgaaagaa
     4861 ctgctaagtg atacttctgt tccagtccaa aaacttgata gttacgtagc tagtttcaat
     4921 agtatggata aattggcact atataataat aaacacgatt ttgcttttgg cctatcaatg
     4981 ttttcgaatc gaactcaaaa ttatgaagct atgaataatg aaaatcttca tggctggttt
     5041 acttctgatg gaatgtttta cctatacaat aacgatttag gacactacag tgaaaactat
     5101 tgggcaactg taaatcccta ccgcttacct ggaaccacag aaactgagca aaaaccacta
     5161 gagggaactc ctgagaatat taaaacgaac tatcaacaag ttggcatgac tagtctctct
     5221 gatgacgctt ttgttgcaag taaaaaactt aataacacaa gtgctctagc tgctatgacc
     5281 ttcactaatt ggaataaaag cctcactctc aataaagggt ggtttatctt aggaaacaaa
     5341 ataatctttg ttggtagcaa tatcaaaaac caatcatctc acaaggcgta tacaactatt
     5401 gaacaacgaa aagaaaatca aaagcaccct taccatgctt atgttaacaa tcaacccgtt
     5461 gacttgaata atcagctagt tgattttaca aacactaaaa gtattttcct tgaaagtgat
     5521 gattctgctc aaaatattgg ttactacttc ttcaagccaa caacacttag cataagtaag
     5581 gcacttcaaa cagggaaatg gcaaaacata aaagctgatg acaaatcacc agaagccatc
     5641 aaagaagttt caaatacctt tatcactatc atgcaaaacc atactcaaga tggcgatcgt
     5701 tatgcctata tgatgcttcc aaatatgact cgtcaagaat ttgaaaccta tattagcaag
     5761 cttgatatcg acttgttaga aaacaatgac aaactggccg ctgtctacga tcatgatagt
     5821 caacagatgc acgtcattca ctatgaaaaa aaagcaacga tgttttcaaa tcataatctt
     5881 tctcatcaag gcttttatag ttttcctcat cctgtcaagc aaaatcaaca ataaaagctt
     5941 gctcatcaag gaattgcagc taaaaacaat gctcttaact ctcataaaat tccccacaaa
     6001 agacaacgac gcttaccgca tactgggtat cagtcctcct ctttagaatt cttaggtgga
     6061 gctttagttg cttcttttaa ccatatcaca aaaccctttc gtaagaaaga cttaaggatt
     6121 tagctgttat tggaaagtaa gggttaacaa tgaaacgtta ttaaagacaa aaaactggaa
     6181 actgcactga gccccaaaag ttggctactc tatttgaagt aaaggattta gttctgtatt
     6241 gtagaggact aagtcctttt cgttttattt tgatacggtt attggtgcaa taatcgatga
     6301 taatagtagg tagaacgagt cactttagcc gtccttaaaa gaaaatctaa tcagagctcg
     6361 tctttgtcca ttctttataa tggttttttg tcttcttctt ttcagagatg cttctttcaa
     6421 acggagttct ctcgtttttt tagcgctaaa tgtctagctt atcttcatag cttaatgtca
     6481 tacaaaaaca ccccaaacgt tagggtttta tatctaacgt ttggggtgca gttcatagtt
     6541 gtgctagtct tttttgtgat ctgttatgtg atgatatttt tcattagtta ataagacaaa
     6601 gacgttttac ttacgttttg gtgggttatg gatagcttcg ccaagcacat ctgcaaaggc
     6661 ttcgtacatc acttcagaga aggttgggtg tccatgaatt gataataaca attcatcaac
     6721 ggttaattca gattccataa tagtagctgc ttcgttaatc atctcagctg cagctggacc
     6781 aataatatga acacctaaga tttcgtggta tttggcatca gcaataactt taacaaaacc
     6841 atgggcttca tttgaagcaa tcgcacgtcc attaccagta aagctgtttt taccaataag
     6901 cacatcacca tattgttcac gcgcttgttc ttcagttaaa ccaaccattg ccacttcagg
     6961 gtgtgtgtaa acggctgctg gagtgtattt aaggttagct ttacgagttg tgttgccatg
     7021 tatcgcattt tctgcagcca cttcacccat acggtaagca gcgtgagcaa gcattttcgt
     7081 tccgttaacg tcacctggcg catagatacc tggaattgat gtctcttggt agtcattaac
     7141 tttgatacgg ttacgatcca tttcaaggtt aagattttcc agaccgttca tttgtggtac
     7201 acgaccgata gaaagaagag ctttttcagc gacaacttct tcaccattgt taagtttcaa
     7261 ggtcaattgg ttatttgctt caacaatttc agagacacca acagatgttt tgatcttcat
     7321 gcctttctta gaaaggattt tttgaagttc aagagagact tccttatcca tagctgggat
     7381 aatgcggtca gccatttcaa taacggtaac atccacaccg taagatgccc aaacaagtcc
     7441 aagctcgata ccaacaacac caccgcccat aactgctagt gattttggca tttcacggag
     7501 gtcaaggata tcgtcagacg ttaagacaag tttagagtca attcctggga tattgatacg
     7561 tgatacttta gaacctgtag caaggataac gttgcgaccc ttaatggttt gtgacccaat
     7621 ggttacagtc ttgtcagggt taacttgacc aagcccatta aagatagtga ctttattagc
     7681 ttttagaaga ccttgaacgc cgcctgttaa ggttttaaca acggtgtttt taaagtcaac
     7741 tgttttgtcc atgtcaatgg tatagttagt tgaggcaagg ttaatcccac gtcctgctgc
     7801 aatcttaata ccatcaagga tttcagcatt tttaaggtaa gttttggttg ggatacaccc
     7861 tacgttcaag caagttccac caaattcaga tttctcaacg atggcaattt taccaccaag
     7921 ttgagcacca cgaatagctg cataataacc agcagggcca ccaccaacaa cgatgatgtc
     7981 atactcatca tcagcaagtg cagcttttgg tgcagctgaa gcagcttgag ctggtgcttt
     8041 tggcacttca agtccagcag cttctaaatc ttctgttgta cgagcaacat tcacatctga
     8101 agcagctggg ctagaaacct caacagattc accttcagca ccgatatatc cgataacttc
     8161 tgttacaggt actgtttcac ctgcttgacg tgtaatttta agaagaacac ctgagtcttc
     8221 tgcttcaagt tccatattgg ttttatctga catgatttca agaagaatat cgccttcatt
     8281 gacagtatca ccttcttgtt ttttccactc gatgatttca ccttcttgca tgtcaacacc
     8341 gagttttggc ataataattt caacagccat aatttaagat accaagagca aggagaaagc
     8401 gattgaattt ttgcggtaag tcagaaatct ttgatttcgt tgacgcaccc acgtcaggac
     8461 gactaggact ttccgaaaca aaaattgagg aaagttcaga cgtctacgac gctaagatta
     8521 gccaactctt accattttta tccgagcttt tcgctcggat tcaattgctt atcactcatt
     8581 tgcatgagat ctcctcgttc tacatccttt ctatattttt gagaaaaggg cacccaccaa
     8641 gtctaaactt agcaaaatcc cttttattat tgaatgcttt tcttgtttaa aactgattga
     8701 tcagattaac aattcaaatg gattttccat caatttcttg agatcaacca tgaattttgc
     8761 accgttcata ccatcaacta aacggtggtc gatggttagg cacattgcca taattggacg
     8821 ggcaacaatc tcaccatcca caaccgttgg agttgggata gttgctccaa caccaagaat
     8881 tgctgagttt ggttggttaa taattgggtt aaatgtcttc gtaccaaaca ttcccaagtt
     8941 tgtgatagag aaggttgatc cagacatttc agctgctttc aacttaccag tttgcgcttt
     9001 tttaatcaca tcttttgaag caagcacaaa atctgacaag ctcatcttgt cagccccatg
     9061 gataacagga acgataagac catcatcaag acctacggca ataccaaggt tcacaaagcg
     9121 gtgtaattca atatcattag catcattgat gagggaagca ttcatgtact catgctcagg
     9181 tttcatcaag gttttaacca ctgccatacc aatcaagtct gtgaagctga ctttaaggcc
     9241 tgttttagcc atgattgggt caattaactt cttacgaagg gcaatcatct ctgtcatgtc
     9301 aatatcgtag ttaagcgtaa aggtaggagc agtaaggtaa gagtttgtca tacctttaga
     9361 gattgcctta cgcatagctg acattggctt gtgttccaca ccttctgcaa gatcaacaac
     9421 tttttcttct ttagctgctg gggcttttgc ttcagctggt ttagccgctt caagaatagc
     9481 catgatatct tctttcatga ccttaccgtt aaagccagta cctgatacgg ttgccaaatc
     9541 aatacctttg tctgctgcaa ttttacgagc aagtggtgaa gctttcggtt gagcaccttt
     9601 aaagttttca acgtctgctt tatggacacg tccttttggt cctgttcctg gaacttggcc
     9661 aaggtcaatg cccatttctg ctgctgcctt acgtgcagct ggagtagcac gaacttttcc
     9721 gccatttcct tgcggaatag ctgtcgctgc cacttgagga gctggacttg ccacattttc
     9781 ttttggagca actgctggtc cagcgtctgc tgaagcagga actggaattt ctgttgtttt
     9841 ttcacttgaa gcaatagtat ctactgattc accctctgca ccaatgtaac cgataacttc
     9901 agttacaggt acagtatcac cagctggacg gacaattttc aatagcacac ctgagtcttc
     9961 tgcctcaagt tccatattgg ttttgtctga catgatttca agaaggatat caccttcatt
    10021 gacggtatca ccttcttgtt ttttccactc gatgatctcg ccttcttgca tgtcaacgcc
    10081 cagctttggc ataataattt caaatgccat ttctacatcc tttcacgatt tgataagcgc
    10141 catcaacatc cccaccttgc tacgaaaaca tacgaaaggt gaggtgccgc gacacgggat
    10201 aagacaactc agttcttcta aaaactttca aaatagtttt tagctgagac gttactgata
    10261 aacatagcct ttatcagacc acttgcctta tcttctagtt gcctttgttt gccattttca
    10321 caatggcagc tttgattttc tcaacgtctg gtaagatcgc ttgttcaagt acacgtgcat
    10381 aaggtactgg aacatcttca cttgcaagac gaacgattgg gtgatcaagg taatcaaagg
    10441 cttcactttc agtaatcatg gttgcaattt ccccaatgaa tccaccggtc ttgtaggcat
    10501 cgttgacaag catcaattta cctgttttct taacagattc aataatcaac tctttatcaa
    10561 gtgggatgag ggtacgtggg tcaacaactt caacgttaat accatccgct gcaacttctt
    10621 cagccgcttg aaggacacgc tccaacatac gaccatatga cacaattgtc aaatcagttc
    10681 cttcacgttt gatgtcacct ttgccaagtg ggatatagaa gtcaggatct tggttaactt
    10741 cttctttttt accataaagc gcttttggtt ccatgaaaag aacaatgtta ttatcacgga
    10801 tagctgattt taataagcct ttagcatcgt tggcattacc aggagcaaca actttgattc
    10861 ctggaatgtg agtcaaccat gcttcaagag attgtgagtg ttgcgcagcg gaaccaatac
    10921 cagaacctga tgccacacgg aaggttacag gggtaataag gcctccaccg aacatgtaat
    10981 tatttttagc accattattc acgatagcgt ccatcatgat ggtaaggaaa tccataaagg
    11041 taacgtcgac aattggacgt agtcctgtaa tagctgcacc aatagccgca ccagagatag
    11101 cagcttctga aattggggta tctttaacac gtttaggacc aaattcttca atcattccaa
    11161 cagaagtccc gaagtctcct ccgtaaaccc ctacgtcttc tcccataagg aaaatgtttt
    11221 cgtccttgcg catttcctca gtcatagcaa ggttgactgc ttcacgcaaa gccattaatt
    11281 ttgtttctga catcattttt atttctccat ttattattca aatacgattg cttcttactg
    11341 acaaggtgat cttagtcaac ccaaacgtct tcgaaggcta ctgaaagttc tggatctgga
    11401 ctgttttgtg caaattcata agcatcgtca acttcttttt tgacttgtgc ttgaatagca
    11461 tccaattcgt catcagtggc aatgccttcg cttgtcagat aagtacgata tttgatcatt
    11521 ggatcttttt ctttccactc atccacttct tctttagtac ggtatttacc agcatctgca
    11581 gtcgagtgac caaaccaacg gtatgattct acctcaacaa tagctggacc atttccacca
    11641 cgaacatgtt caacggcttt gcccattgtt tcatagacag ccatcacgtc attgccatct
    11701 tcacagtaga aaccaggaat tccgtaagct tctgcacgtg tgtataaatg tggtgtattt
    11761 gttgcattat tgatgctcat tgaaattcca taacggttgt tgatgatgaa gaaaataaca
    11821 ggaagtttcc atgttgcagc catattaaca gactcgtgga atgatccttc gttagtagcg
    11881 ccatcacctg agaaagcaac agctatattg ttagttcctt tgtattgttg ggtgagggca
    11941 gcaccaactg caagagcgta accaccacca acgataccat tggtaccata gtttcctttt
    12001 tcaaaatcag ccaagtgcat tgagccacca cgccctttag aaacaccagt cgctttacca
    12061 gcaagctcag ccatcatttt attaaggtcc atgtctttag cgattgattg tccatgtcca
    12121 cggtgatttg aaaaaataat gtcatcgtaa gacaagtgag ccaccgctcc aacattggct
    12181 gcttcttcac caactgagaa gtgagtcatg ccttgtacaa atccacgacg gacgagttta
    12241 ttaatacgtg aatcaaattc acgaatacgt tccattttaa ggaacatatc caaatgttgt
    12301 tctttagaaa ctgttaccat ttcggcctcc atattatcat attacctttt tatgataaaa
    12361 ctttttttca caatttgcaa ataattaaat cgctttctac cttaccttat ttggtttttt
    12421 agaaatgcaa tagtttgtg
//
LOCUS       DATXIG010000042        12231 bp    DNA     linear   BCT 28-DEC-2023
DEFINITION  TPA_asm: Streptococcus pyogenes strain MGAS11113
            SAMN08555799-rid19682583.denovo.042, whole genome shotgun sequence.
ACCESSION   DATXIG010000042 DATXIG010000000
VERSION     DATXIG010000042.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN08555799
            Sequence Read Archive: SRR6740495
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pyogenes
  ORGANISM  Streptococcus pyogenes
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 12231)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12231)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-OCT-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-OCT-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001918058.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001918058.2
            Genome Coverage       :: 368x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/12/2023 11:23:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 1,806
            CDSs (total)                      :: 1,769
            Genes (coding)                    :: 1,722
            CDSs (with protein)               :: 1,722
            Genes (RNA)                       :: 37
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1, 1 (5S, 23S)
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 47
            CDSs (without protein)            :: 47
            Pseudo Genes (ambiguous residues) :: 0 of 47
            Pseudo Genes (frameshifted)       :: 30 of 47
            Pseudo Genes (incomplete)         :: 20 of 47
            Pseudo Genes (internal stop)      :: 9 of 47
            Pseudo Genes (multiple problems)  :: 11 of 47
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12231
                     /organism="Streptococcus pyogenes"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN08555799-rid19682583.denovo.042"
                     /strain="MGAS11113"
                     /serotype="Emm28"
                     /isolation_source="normally sterile site"
                     /host="Homo sapiens"
                     /db_xref="taxon:1314"
                     /country="Finland"
                     /collection_date="2002"
                     /collected_by="National Public Health Laboratory"
     gene            394..822
                     /gene="lacA"
                     /locus_tag="VC122_001267"
     CDS             394..822
                     /gene="lacA"
                     /locus_tag="VC122_001267"
                     /EC_number="5.3.1.26"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500922.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="galactose-6-phosphate isomerase subunit LacA"
                     /protein_id="HEP5641191.1"
                     /translation="MAIIIGADKAGQELKEVIKDYLKEGKYEVVDVSENEVRDFVDTT
                     LAVAKEVNASEDNLGIVIDAYGVGSFMVATKIKGMVAAEVSDERSAYMTRGHNNARII
                     TLGSEISAPGIAKNIIKGFVEGKYDGGRHQVRVDMLNKMC"
     gene            857..1372
                     /gene="lacB"
                     /locus_tag="VC122_001268"
     CDS             857..1372
                     /gene="lacB"
                     /locus_tag="VC122_001268"
                     /EC_number="5.3.1.26"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012027025.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="galactose-6-phosphate isomerase subunit LacB"
                     /protein_id="HEP5641192.1"
                     /translation="MKIAVGCDHIVTYEKIAVVDYLKSQGHEIIDCGTYDNVRTHYPI
                     FGKKVGEAVASGEAELGVVICGTGVGITNAVNKVPGIRAALVRDMTSAIYSKEELNAN
                     VIGFGGKIIGGLLMNDIIDAFLAAEYKPTEENKKWIEKMDSLQHASQDQNNPHFFDEF
                     LEKWDRGEYHD"
     gene            1418..2347
                     /locus_tag="VC122_001269"
     CDS             1418..2347
                     /locus_tag="VC122_001269"
                     /EC_number="2.7.1.144"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018379688.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tagatose-6-phosphate kinase"
                     /protein_id="HEP5641193.1"
                     /translation="MILTVTLNPAIDVSYPLNELKCDTVNRVVDVTKTPGGKGLNVSR
                     VLNDFGETVKATGCIGGESGDFIINHLPDSILSRFYKISGDTRTCIAILHEGNQTEIL
                     EKGPLLSVDEIDGFTHHFKYLLNDVDVVTLSGSLPAGMPDDYYQKLIKIANLNGKKTV
                     LDCSGNALEAVLKGDSKPTVIKPNLEELSQLLGKEMTKDFEALKEVLQDELFEGIEWI
                     IVSLGADGVFAKHKDTFYNVDIPKIKIVSAVGSGDSTVAGIASGLANDEDDRALLTKA
                     NVLGMLNAQEKTTGHVNMANYDKLYQSIKVKEV"
     gene            2350..3333
                     /gene="lacD"
                     /locus_tag="VC122_001270"
     CDS             2350..3333
                     /gene="lacD"
                     /locus_tag="VC122_001270"
                     /EC_number="4.1.2.40"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017768297.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tagatose-bisphosphate aldolase"
                     /protein_id="HEP5641194.1"
                     /translation="MTITLTENKRKSMEKLSIDGVISALAFDQRGALKRMMAQHQTKE
                     PTVEQIEELKSLVSEELTPFASSILLDPEYGLPASRVRSEEAGLLLAYEKTGYDATTT
                     SRLPDCLDVWSAKRIKEAGAEAVKFLLYYDIDGDQDVNEQKKAYIERIGSECRAEDIP
                     FYLEILTYDEKIADNASPEFAKVKAHKVNEAMKVFSEERFGVDVLKVEVPVNMKFVES
                     FADGEVLFTKEEAAQAFRDQEASTDLPYIYLSAGVSAKLFQDTLVFAAESGAKFNGVL
                     CGRATWAGSVKVYIEEGPQAAREWLRTEGFKNIDELNKVLDKTASPWTEKM"
     gene            3357..3674
                     /locus_tag="VC122_001271"
     CDS             3357..3674
                     /locus_tag="VC122_001271"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006739201.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS lactose/cellobiose transporter subunit IIA"
                     /protein_id="HEP5641195.1"
                     /translation="MNREETTLLGFEIVAYAGDARSKFLEALKAAQEGDYAKAEELIA
                     AGSDCLNDAHNAQTSLLQKEAAGDDLAYSVTLMHGQDHLMTTILLQDLMKHMIELYKR
                     GAK"
     gene            3674..5371
                     /locus_tag="VC122_001272"
     CDS             3674..5371
                     /locus_tag="VC122_001272"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011285167.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit EIIC"
                     /protein_id="HEP5641196.1"
                     /translation="MNGLIAQIEKGKPFFEKISRNIYLRAIRDGFIAGMQVILFSSIF
                     ILIAYVPNAWGFHWSKDIENLLMTPYNYSMGILGLFVAGTTAKALTDSMNRSLPSTNQ
                     INFISTMLAAIVGFLLMAANPAKDGGFLTGFMGTKGLLTAFIAAFITVNVYKTCVKNN
                     VTIRMPEEVPPNISQVFKDLIPFTLSVVLLYVIELIVHTTLGVNVAESIGKLLAPLFQ
                     AADGYVGITIIFGAFAFFWFVGIHGPSIVEPAIAAITYANAETNLQLLQAGEHADKIL
                     TSGTQMFIVTMGGTGATLVVPFMFMWLTKSKRNKAIGRASVVPTFFGVNEPILFGAPL
                     VLNPIFFIPFILAPIVNVWIFKFFIDTLGMNSFTANLPWTTPAPLGLILGTNFQVLAF
                     ILAALLIVVDVIIYYPFLKVYDEQILAEEAAGTNSSDALKEKVAANFDTKKADAILEK
                     SAAKETKEITDQTNVLVLCAGGGTSGLLANALNKAAKEYGAPVTAAAGSYGAHREILP
                     QYQLVILAPQVASNYEDMKVETDKLDIKLAKTEGAQYIKLTRDGQGALDFVKEQMEK"
     gene            5444..6850
                     /gene="lacG"
                     /locus_tag="VC122_001273"
     CDS             5444..6850
                     /gene="lacG"
                     /locus_tag="VC122_001273"
                     /EC_number="3.2.1.85"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288409.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6-phospho-beta-galactosidase"
                     /protein_id="HEP5641197.1"
                     /translation="MTKTLPKDFIFGGATAAYQAEGATHTDGKGPVAWDKYLEDNYWY
                     TAEPASDFYNRYPVDLKLSEEFGVNGIRISIAWSRIFPTGKGEVNPKGVEYYHNLFAE
                     CHKRHVEPFVTLHHFDTPEALHSDGDFLNRENIEHFVNYAEFCFKEFSEVNYWTTFNE
                     IGPIGDGQYLVGKFPPGIQYDLAKVFQSHHNMMVSHARAVKLFKDSGYSGEIGVVHAL
                     PTKYPFDANNPDDVRAAELEDIIHNKFILDATYLGKYSDKTMEGVNHILEVNGGELDL
                     REEDFAALDAAKDLNDFLGINYYMSDWMQAFDGETEIIHNGKGEKGSSKYQIKGVGRR
                     KAPVDVPKTDWDWIIFPQGLYDQIMRVKADYPNYKKIYITENGLGYKDEFVDNTVYDD
                     GRIDYVKKHLEVISDAISDGANVKGYFMWSLMDVFSWSNGYEKRYGLFYVDFETQERY
                     PKKSAYWYKKVAETQVIE"
     gene            7353..7508
                     /locus_tag="VC122_001274"
     CDS             7353..7508
                     /locus_tag="VC122_001274"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002982773.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type A2 lantipeptide"
                     /protein_id="HEP5641198.1"
                     /translation="MSFMKNSKDILTNAIEEVSEKELMEVAGGKKGSGWFATITDDCP
                     NSVFVCC"
     gene            7590..9215
                     /locus_tag="VC122_001275"
     CDS             7590..9215
                     /locus_tag="VC122_001275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012561003.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4135 domain-containing protein"
                     /protein_id="HEP5641199.1"
                     /translation="MIKRNELKLEYQQHQFYEYFNSIFDYDILDTSISENIYLLREKT
                     HKLFYSEFENQLFETIMFLSMKTLVLDINHFSKEIENKSEAYEQCIQQIREENGINHF
                     FDRYPYLLKQINKEVGLIEESYSLLFDRFLEDLSEIRSCFNISEPLSNVAFSLGDSHS
                     KKQTVVKIAFKEKSVYYKPKSYHSHSILLELTSLLKSSNIPSFSLPKSLVKADYCWQL
                     GVAYTSSNKDEVAKIYFKYGVLAAFSEIFSITDLHMENVIVSGGDLYLIDVETFFQRK
                     LNVQNQNFEGITVDTYQRIYETSLSNGLFPVQFEKNSAPNVSGISGKGGKRKKGKYEL
                     INKNRGDMKLVKVDYFQEDGFNIPTLNGKVVEPLDYANEIISGFRECYIFLLSQRSKI
                     KGIVEGFPELKSRALFRNTSDYGKFLQASTNPKYLFSEKKKKNLFSILYEAKHIEHFI
                     VDNEIKDLMNGDIPYFSMDTRGNVYNSVGTLIGNLGDTTSLFDSITILNDERLKFTCE
                     LLEIVLKKPIKHWEREKGKSYQFLSISSEHYLL"
     gene            9217..9750
                     /locus_tag="VC122_001276"
     CDS             9217..9750
                     /locus_tag="VC122_001276"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011527761.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEP5641200.1"
                     /translation="MDKTDFRNISAFVSQDSPMFDGDVMYNISLGRESVSGEQVIETC
                     KRVSIYDDIRSMPMKFHTPLFRDNPSLSGGQKQRISLARELVTTPRILVLDEPTSALD
                     VKTERIIQKNVEALHCTRILVTHRLNTVEKADKILIMDNGKIIDYGNHHYLYKNNKDY
                     CDLYDSYMNKYQEEEVK"
     gene            9747..10484
                     /locus_tag="VC122_001277"
     CDS             9747..10484
                     /locus_tag="VC122_001277"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000812013.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEP5641201.1"
                     /translation="MKQLVLKDVCKKYPNQLNYALDHINLIVEKGEFVAVMGRSGSGK
                     TTLLNVTSIIDKIDSGSIYCADKEISAFSDNEATSFRKNDIGFVFQDYMLLDSLTIRE
                     NISVALSLKNVDSSKIDDLINNYAKRFNLYEQLKKYPYQLSGGQRQRVSIIRAIIKEP
                     EIIFADEPTGALDLKSSEGTMRILSEINKIEKVTILMVTHDVLSASYADRVVLLKDGK
                     LHMEIDKEDCGETFYDVISQALSDRGE"
     gene            10486..>12231
                     /locus_tag="VC122_001278"
     CDS             10486..>12231
                     /locus_tag="VC122_001278"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012561001.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEP5641202.1"
                     /translation="MIWSITKSNIKKNFSLYRIYFLATIGLLSIFIAFLNFISDKIIT
                     EKIGDSGQALVIANGSLVFLTVFLVIFLIYFNNFFVKKRSQELGVLAILGFSKKELTQ
                     LLTLENLVILVLSYLVSLLLGPTLYFLAVLVITHLLELTLEVQWFITANEIIESLGIL
                     VVVFLINVITNGLIISKQSLIEFVNFSRKAEKKIKIRKVRAIIAITALLLSYILCLAT
                     VFSSTRNMLLSIGMVPVSLLIIVLVVLGTVFTIRYGLAFIVSLLKGNKKRLYRPLSNI
                     IYPKFNYRIATKNKLLTVLGGLLTVTVSVAGMMVMLYAYSLNGIERLTPSAIEYNVKS
                     ENGQVNVTTILENDQVSLVDVDLLRLNTNPEVTITESGQTIPYFDIINYSDYKELMKA
                     QGRINSIEGSKSLPLLINYYPTEISLGKTFNLGNAYDVTVKQVSTHNVFSFSTSVTTL
                     VVSDKLYAKLSSCFPEKEMTIRTFNGNSIRSSEAFYNQFSMVPDVISSYSKEYTVKTA
                     NIATYIFITFLSILFIICTGSILYFTSLIEIMENKEEYGYLSKLGYSKKMIHRIPRYE
                     TGILFLIPVFIGIVNG"
BASE COUNT         4152 a         1818 c         2370 g         3891 t
ORIGIN      
        1 aatatcaata aaaaaatcaa acaaaacaaa cataaacatt ggaaaaaaca aaaattaaca
       61 aaatagaaaa tgttttaaat aaaacgttta caataaatag agataagggt ctcgtaatat
      121 atgataggtt acgagtggct gtcaaaggga tttatgaaat gaaaaagaaa cacaagaaac
      181 attaggataa taacgtaaaa atgaaaaaaa taaacatatt ttgtttattt tttataaaaa
      241 taaacaaaaa aatgttgact tttatgtttg gtgggtgtat gattaggtca tagaaataat
      301 gaaagcgtta tttcaaaaga tgttgcaaat cgttaattaa ctatctacgt aggttttcca
      361 catttactta ttctaaatca taggagaaat aaaatggcaa ttattatcgg tgctgacaaa
      421 gcaggtcaag aacttaaaga agttatcaaa gattacctta aagaaggtaa atacgaagtc
      481 gttgatgtta gtgaaaacga agtacgtgac tttgtagata caactttagc cgttgcaaaa
      541 gaagttaatg cctccgaaga taacttaggt attgtcattg atgcatatgg tgttggttct
      601 ttcatggtag ctactaaaat caaaggaatg gtggcagctg aagtatcaga tgagcgttca
      661 gcatacatga caagaggaca caacaatgct cgaatcatca cgcttggttc tgaaattagt
      721 gctccaggaa ttgcaaagaa cattatcaaa ggctttgtcg aaggtaaata tgatggtgga
      781 cgtcatcaag ttcgtgtcga catgcttaat aaaatgtgct aattgattga aataggttaa
      841 aacaaggaga acaaaaatga aaattgctgt aggttgtgac catattgtta cttatgaaaa
      901 aatcgctgtt gtggattacc taaaatcaca aggtcatgaa atcattgatt gcggtacata
      961 cgataatgtt cgcacacact atccaatctt tggtaaaaaa gttggtgaag cagtagcaag
     1021 tggtgaagca gaattaggtg ttgttatctg tggtactggt gttggtatca caaatgctgt
     1081 aaacaaagtt cccggtattc gtgcagcact cgttagagat atgacatctg ctatctactc
     1141 aaaagaagag ctaaacgcaa acgttattgg ttttggtgga aaaatcatcg gtggtttgct
     1201 gatgaatgat attatcgatg ctttcttagc ggctgaatac aaaccaactg aagaaaataa
     1261 aaaatggatt gaaaaaatgg atagcttgca acatgctagc caagatcaaa acaacccaca
     1321 cttctttgat gaatttctag aaaaatggga ccgtggtgaa tatcacgact aatatttgaa
     1381 agaattatga taaggatata tagaaaaagg agctattgtg attttaacag ttactttaaa
     1441 tccagctatt gacgtctcct accctttgaa tgagctaaaa tgcgatactg tcaatcgagt
     1501 tgtagacgtt acaaagacac ccggtggtaa aggcttgaat gtttcccgag ttctaaatga
     1561 ctttggtgag acagtcaaag ctactggttg tattggtgga gaaagcggtg attttatcat
     1621 taatcatctt cctgattcga ttttaagtcg cttctataaa atttcaggtg ataccagaac
     1681 atgtatagcc atattacacg aaggtaatca aactgagatt ttagaaaaag gaccgctgtt
     1741 aagtgtagat gaaattgatg gatttactca tcattttaaa tatttactga atgatgttga
     1801 cgtagtgacg ctctcaggca gtttgcctgc tggtatgcca gatgattact atcagaagtt
     1861 aatcaagatt gccaatctta atggtaagaa gactgttctg gattgttctg gaaatgcttt
     1921 agaagcagta ttgaaaggtg atagtaaacc aacagttatc aaacctaatc ttgaagaact
     1981 ttctcaactt ctaggtaaag aaatgacaaa agattttgaa gccttaaaag aagtcttaca
     2041 agatgaacta tttgaaggaa ttgaatggat aatcgtttct cttggtgctg atggtgtatt
     2101 tgcaaagcac aaggatacgt tttataacgt tgacattcct aaaatcaaaa ttgtaagcgc
     2161 tgtaggttcg ggtgattcta cagtggcagg tattgcttca ggtttagcga atgatgaaga
     2221 tgatcgagct ttattaacaa aagcaaatgt cttaggtatg ttaaatgcgc aagaaaaaac
     2281 aactgggcat gttaacatgg caaattacga taagttatat caatctatta aagtcaaaga
     2341 ggtataaaaa tgacaattac tttaactgaa aacaaacgta aaagcatgga aaaactaagt
     2401 atagatggtg ttatttctgc tttagctttt gaccaacgtg gagctctcaa acgtatgatg
     2461 gctcaacatc aaacaaaaga gccaacagta gaacaaattg aagagcttaa aagtttagtt
     2521 tctgaagaac tgacaccatt tgcatcatca attttacttg atcctgaata tggcttgcca
     2581 gcaagtcgtg ttcgttcaga agaagcaggc ttgttgttgg catatgagaa aacaggatac
     2641 gatgcaacaa caactagtcg cttacctgat tgcttagatg tttggtctgc aaaacgtatt
     2701 aaagaagctg gagcagaagc agttaaattc cttctttact atgatattga tggagatcaa
     2761 gatgttaacg agcagaaaaa agcttacatt gaacgtattg gttctgaatg ccgtgcagaa
     2821 gatattccat tctacttaga aattctaact tatgatgaga aaattgctga caatgcaagc
     2881 cctgagtttg caaaagttaa agctcataaa gttaatgaag caatgaaagt cttctcagaa
     2941 gaacgctttg gtgttgacgt cttgaaagtc gaagttccag taaatatgaa atttgtagaa
     3001 agctttgcag atggtgaggt tctctttaca aaagaagaag cagctcaagc tttccgtgac
     3061 caagaagctt ctactgattt accatatatt tacttgagtg caggtgtttc tgccaaattg
     3121 ttccaagata ctttggtgtt tgcagcagaa tcaggtgcta aattcaacgg tgtcttatgt
     3181 ggacgtgcta cctgggcagg atctgtaaaa gtctatatcg aagaaggacc acaagcagct
     3241 cgtgaatggt tgcgtacaga aggtttcaaa aatattgacg aattaaacaa agtactagat
     3301 aaaactgcaa gtccttggac tgaaaaaatg taacattatt ttcggaggaa actacaatga
     3361 atagagaaga aactactctt ttaggttttg aaatcgtagc ctatgctggt gatgcccgtt
     3421 caaaattttt ggaagcgctt aaagcagcgc aagaaggaga ttatgctaaa gcagaagaat
     3481 taatcgctgc aggtagtgac tgcttgaatg atgctcacaa tgctcagaca agtctactac
     3541 aaaaagaagc agctggagat gacttagctt atagtgtcac tttaatgcat ggtcaagatc
     3601 atttaatgac gaccatttta ttacaagatt taatgaaaca catgattgaa ctttataaga
     3661 gaggagcaaa ataatgaatg gattaattgc tcagattgag aaagggaaac ctttcttcga
     3721 gaaaatttct cgaaatatct atcttcgcgc tattcgtgat ggttttatcg ctggtatgca
     3781 agttatcttg ttttcaagta tctttatctt gattgcctat gttccaaatg catggggctt
     3841 ccattggagt aaggatattg agaatctctt gatgacgcca tacaactact caatgggtat
     3901 tttaggtttg tttgtggctg gtacgacagc caaagcttta actgactcaa tgaatcgatc
     3961 attaccaagc acaaaccaaa tcaactttat ttcaactatg ctcgctgcta ttgtaggttt
     4021 tttacttatg gcagccaatc cagcaaaaga cggtggtttc ttaactggat ttatgggaac
     4081 aaaaggtttg ttgacagcct tcatcgcagc ttttattacc gtaaatgtct ataaaacctg
     4141 tgtgaaaaac aatgttacta ttcgcatgcc agaagaggtt ccaccaaata tatctcaagt
     4201 ttttaaagat ttgattccat ttacgttatc agttgtactg ctttatgtga ttgaattgat
     4261 tgttcatact actttaggcg ttaacgttgc agaatcaatt ggtaaacttc ttgcaccatt
     4321 gttccaagca gcagatggct atgttggaat cactattatc ttcggtgcat ttgctttctt
     4381 ctggtttgtt ggtattcatg gaccatctat tgttgaacca gccattgcag cgattactta
     4441 cgcaaatgct gaaaccaacc ttcaattact tcaagctggt gaacatgctg ataagatctt
     4501 gacttcaggg actcaaatgt tcatcgttac catgggtggt actggtgcaa ctctcgtcgt
     4561 tccattcatg ttcatgtggt taacaaaatc aaaacgtaac aaagccattg gacgtgcatc
     4621 agttgttcca acattctttg gtgttaacga acccattttg tttggtgccc cacttgtttt
     4681 gaacccaatc ttctttattc catttatcct ggcaccgatt gtcaacgtat ggattttcaa
     4741 attcttcatt gacacacttg gaatgaacag tttcactgca aacttacctt ggacaacacc
     4801 agctccattg ggtcttatcc ttgggacaaa cttccaagtg ctagcattca ttttggcagc
     4861 tttgttaatt gttgttgatg tgattatcta ctacccattc ttaaaagtat atgatgagca
     4921 aattcttgct gaagaagcag caggtacgaa ttcatcagat gccctcaaag aaaaagtagc
     4981 agcaaacttt gatactaaaa aagctgatgc tattcttgaa aaatcagcag ctaaagaaac
     5041 caaagaaatc actgatcaaa ctaatgttct cgttctttgt gccggtggtg gtacaagtgg
     5101 tttgcttgca aacgctttga ataaagcagc taaagaatat ggtgcacctg tcactgcagc
     5161 agcaggaagt tatggtgctc accgtgagat tttaccacaa tatcaacttg ttatccttgc
     5221 acctcaagta gcttctaact atgaagatat gaaagtggaa actgataaac ttgatattaa
     5281 attagctaaa acagaagggg ctcaatacat caaattgact cgcgatggtc aaggcgcatt
     5341 agacttcgtt aaagaacaaa tggaaaaata atatgtcgct agggaaggtt gggggtactc
     5401 aatcttcctt tatagtgaaa tgaaaattta gaagaggtat atcatgacta aaacattacc
     5461 taaagatttt atttttggtg gtgctacagc tgcttaccag gctgaaggcg ctacccacac
     5521 agatggtaaa ggaccagtag cttgggataa atacttagaa gacaactatt ggtacacagc
     5581 tgagccagca agtgattttt acaatcgtta tccagtcgat ttgaaactta gtgaagaatt
     5641 tggtgtcaac ggcatccgta tctctattgc ctggtctcgt atttttccaa caggaaaagg
     5701 agaagttaac cctaaaggag ttgaatacta ccacaatctc tttgcagagt gtcataagcg
     5761 tcatgttgag ccttttgtta cacttcacca ttttgatacc ccagaagctc tccactcgga
     5821 tggtgatttc ctcaatcgtg agaacattga acattttgta aattatgcag aattttgttt
     5881 taaagaattc tcagaagtta actattggac aacatttaac gaaattgggc ctattggtga
     5941 tggccaatac ttggttggta aattccctcc aggtatccaa tatgatcttg ctaaagtttt
     6001 ccaatcacac cataacatga tggtctctca tgctcgtgca gtcaaactct ttaaagatag
     6061 tggctattca ggtgaaatcg gcgtggtcca tgctcttcca actaagtatc catttgacgc
     6121 taacaatcct gatgatgtta gagcagctga actggaagat atcatccata ataaatttat
     6181 tcttgatgca acttatctag gtaagtattc agataaaaca atggaaggtg ttaaccatat
     6241 ccttgaggta aatggtggtg aacttgatct tcgtgaggaa gattttgctg cactagacgc
     6301 tgcaaaagat ttgaatgact tccttggtat taactattat atgagtgatt ggatgcaagc
     6361 ttttgatggt gagactgaaa tcattcataa tggtaaaggg gaaaaaggca gctctaaata
     6421 ccaaatcaag ggtgttggtc gaagaaaagc acccgttgat gttccaaaaa cggactggga
     6481 ctggattatc ttcccacaag gcttatatga tcaaatcatg cgtgtcaaag ccgattatcc
     6541 taactacaag aaaatttaca ttacagagaa tggtcttggc tacaaagatg agtttgtaga
     6601 taatactgtc tatgatgatg gacgtatcga ttatgtgaaa aaacacttag aagttatttc
     6661 tgatgctatt tctgatggtg caaatgttaa aggatacttt atgtggtcac tgatggatgt
     6721 cttttcatgg tcaaatggct atgaaaaacg ttacggtctc ttctatgtcg attttgaaac
     6781 tcaagaacgt tatcctaaga agagtgccta ctggtataaa aaagtagcag aaactcaagt
     6841 gattgaatga ttttgagata cttttttaat acatttttac acttcatgtt cgtttactga
     6901 attatagtca caaaaagtag atgatgttga catttttcaa gcatagtatt atttgctgtt
     6961 gatggtaatc cattagttgt aagatcgggt tataagtgtt tttagttgat gaaaatgggt
     7021 tttgaaagtt ttatttaata tcctttttca gatatttgac ttctgtcatt caaactcctg
     7081 acctgattaa ataagtaaag taaccatttt ctgattagag attgatgtta taacaagaag
     7141 cgattagatt gtaaaatagc tgtaaagaac gagttttgtt ttttatatca gagggctatt
     7201 tgttataaag tgacctctta tgtgacatct caaaaatctt actaaaaaat atgaaaaaag
     7261 tcatttttgc actttttact cattgaaaat gaagacgtat tatttacaat aaaagtgtag
     7321 aaaatagtca ctacatactg atagaaagga gaatgagttt tatgaaaaat tcaaaggata
     7381 ttttgactaa tgctatcgaa gaagtttctg aaaaagaact tatggaagta gctggtggta
     7441 aaaaaggttc cggttggttt gcaactatta ctgatgactg tccgaactca gtattcgttt
     7501 gttgttaatt aaaacaatct ggataagcag actaaaattt cctgtttaga cttagtctag
     7561 gcaggatttt taagtagtgg taggaaaaaa tgataaaaag aaatgaatta aagttagaat
     7621 atcagcaaca tcagttttac gaatacttta actcaatttt tgattacgac atattagata
     7681 cttctattag tgaaaatatt tatttgttaa gggaaaagac acataagtta ttttatagtg
     7741 agtttgaaaa tcagttattt gaaacgatca tgttcctttc gatgaaaaca ctagttttag
     7801 atataaatca tttttccaag gaaattgaaa ataagtcaga ggcatatgag cagtgtatac
     7861 agcagataag agaagaaaac ggaataaatc atttttttga caggtatccg tatttgctaa
     7921 aacaaattaa taaagaagta ggactgatag aggaatcata ttctttatta tttgatagat
     7981 ttttagaaga cttatctgag ataaggtcgt gctttaacat tagtgagccc ctaagcaatg
     8041 ttgccttttc acttggtgac agtcactcta aaaagcaaac agttgttaaa attgcgttta
     8101 aagagaaatc tgtttactac aagcctaaaa gttatcattc acacagtatc ttgttagaac
     8161 ttactagttt attgaaatct agtaatatac cttcattcag tcttcctaaa agcttagtta
     8221 aagcagatta ttgttggcaa cttggtgtag cttatacaag ttccaataaa gatgaagtag
     8281 cgaaaattta ctttaagtat ggtgttttag cagcattttc tgaaatattt tctattacgg
     8341 acttacatat ggaaaatgtg attgtttcag gcggagattt atatttaatt gacgttgaaa
     8401 cattttttca aagaaagttg aatgtacaaa atcagaattt tgaagggatt actgtagaca
     8461 cctatcagag aatttatgag acatcacttt caaacggttt atttcctgta caatttgaga
     8521 aaaacagcgc tcctaacgtt tctggaataa gtggaaaagg tggaaaaaga aaaaaaggaa
     8581 aatatgaact gataaataaa aatcgtggag atatgaagtt agtaaaagtt gactattttc
     8641 aagaagatgg gttcaatatt cccacattaa atgggaaagt ggtagagcct cttgactatg
     8701 ctaatgagat catttcagga tttagagagt gctacatatt tcttctatca caacgttcaa
     8761 agattaaagg aatagtagaa ggttttccag aattgaaaag cagagcccta tttcgaaata
     8821 cttccgatta tggaaaattt ttgcaagctt ctacgaatcc aaagtatctt ttctcagaaa
     8881 aaaagaagaa gaatttattt tcgatactat atgaggcaaa acatattgaa cattttattg
     8941 ttgataatga aattaaagat ttgatgaatg gtgatattcc ttacttttca atggatacca
     9001 gaggaaacgt ctataattct gtagggacat taattggaaa tctaggagat acaacctcgt
     9061 tatttgatag tataactatt ttgaacgatg aaagattgaa gttcacttgt gaattgcttg
     9121 aaatagtctt gaaaaaaccg attaagcatt gggagaggga aaagggcaaa tcttatcagt
     9181 ttttatcaat ctcctcagaa cattaccttc tctaaaattg ataaaactga ttttaggaat
     9241 atatcggctt ttgtatctca agattctccc atgtttgatg gggatgtgat gtataacatt
     9301 tcgctaggga gagaatctgt ttcaggagaa caggttattg aaacttgtaa aagggtatca
     9361 atatatgatg atatcaggag tatgccaatg aagtttcata ccccactttt tcgagataat
     9421 ccatcactat ctggggggca aaaacaacga atttctttag caagagaact tgtaaccacc
     9481 cctagaatct tagttcttga cgaacctaca tcagctttag atgtaaaaac tgaaagaata
     9541 atccaaaaaa atgttgaggc tttacattgt acgaggattt tggttaccca tagacttaat
     9601 acagttgaaa aagctgataa gattttaata atggataatg gcaaaattat tgactatgga
     9661 aaccatcatt atttgtacaa aaataataag gattattgtg acttatatga ctcgtatatg
     9721 aataaatatc aggaggaaga ggtaaaatga aacaattagt attaaaagat gtttgcaaaa
     9781 aatatcccaa tcaactgaat tatgctctag accatatcaa tttgattgtg gaaaaaggag
     9841 aatttgtagc tgtcatgggg cgtagtggta gtggaaaaac aacactttta aatgttactt
     9901 caattattga taaaattgat agtgggagta tttattgtgc agacaaagaa attagtgcat
     9961 tttctgataa cgaggcaact agttttagaa aaaatgatat tggttttgtt tttcaagatt
    10021 atatgctgtt agacagtcta accattaggg agaatatttc tgttgctcta tctttaaaaa
    10081 atgttgattc atcaaaaatt gatgatttga taaataacta tgcaaaaaga tttaatttat
    10141 atgagcagtt gaaaaaatat ccgtatcaac tttctggagg tcaaagacag agggtcagta
    10201 ttatcagagc aatcattaaa gaaccagaga ttatttttgc tgacgaacca actggagcgc
    10261 ttgacttaaa atcttctgaa ggaacaatga ggattttgag tgaaatcaat aaaatagaaa
    10321 aggtaactat tttaatggtt actcatgacg ttttatcagc ttcttatgcg gatagagttg
    10381 tacttctcaa agatggtaaa cttcacatgg agattgataa agaggattgt ggagagacgt
    10441 tttatgatgt aattagtcaa gccttatccg atagaggaga gtaatatgat ttggtccatt
    10501 acaaaatcta acatcaaaaa aaacttttcg ttatatcgta tctattttct agctacgatt
    10561 ggtttattaa gtatttttat agcttttcta aattttatct cagataaaat cattacagaa
    10621 aaaattgggg atagtggtca agctctagtt attgctaacg ggtcattagt ttttttgact
    10681 gtatttttgg tgatattttt aatttatttc aataatttct ttgtaaaaaa acgtagtcaa
    10741 gagcttggag tcttagcaat actagggttt tcaaaaaaag aattaacaca attactgact
    10801 ttagaaaatc ttgttattct agttttaagt tacttggtaa gtttactatt gggaccgacc
    10861 ttatattttt tagcggtact ggtaattact catctattgg aattaacatt ggaagttcag
    10921 tggtttatta cagctaatga gattatagag tctttaggaa tattagtcgt agtttttctg
    10981 attaatgtca tcacaaatgg acttatcatt agtaaacagt ctttgattga atttgttaat
    11041 ttctcaagaa aggctgagaa aaaaattaag ataagaaaag tcagagctat tattgctatc
    11101 actgcattgc tattgtcata tattttatgt ttggcgacag tgttttcatc cacacgaaat
    11161 atgctattaa gcatagggat ggtaccggtt tctctattga taattgtctt agttgtttta
    11221 ggaacagtgt tcaccatcag atatggattg gcttttatag tttcgttatt aaaaggaaat
    11281 aaaaaaaggt tataccgtcc tctgtctaat atcatctatc ccaaatttaa ctatcgtatt
    11341 gcaacaaaaa ataaattatt aacagtcttg ggaggtcttt taacagtaac cgtttcagtt
    11401 gctggaatga tggtaatgct ctatgcttat tctcttaatg ggatagagag gttgactcca
    11461 tctgccatag aatataatgt taaatcagaa aacggtcaag tcaatgttac aactatttta
    11521 gagaacgacc aagtgagctt ggttgatgtt gacctattga gattgaacac taacccagaa
    11581 gtaactatca cagaatctgg gcaaacaatt ccttattttg atataattaa ttacagtgat
    11641 tataaagagt taatgaaagc tcaagggaga ataaattcta ttgaaggtag taagtcactc
    11701 ccattgttaa taaattatta tccaacagaa attagccttg gaaaaacctt taacttagga
    11761 aatgcatatg atgttactgt aaaacaagta tcaacgcata atgtttttag tttttctaca
    11821 agtgtcacga ccttggttgt ttctgataaa ttatatgcta aacttagttc ttgttttcca
    11881 gagaaagaaa tgacaattag gacttttaat ggaaattcga ttaggtcaag tgaagcattt
    11941 tacaatcagt ttagtatggt tcctgatgtt atcagtagtt atagtaagga atacacagta
    12001 aagactgcta atattgcgac ttatatcttt ataactttcc tatccatact ctttattatt
    12061 tgtacaggta gtattctgta ctttacaagc ctcatcgaaa tcatggaaaa taaagaagaa
    12121 tatggctatc taagtaagct aggttatagt aaaaaaatga ttcatcggat tcctcgatat
    12181 gaaacaggta tacttttcct tattcctgta ttcattggga ttgtaaatgg t
//
LOCUS       DATXIG010000043        11431 bp    DNA     linear   BCT 28-DEC-2023
DEFINITION  TPA_asm: Streptococcus pyogenes strain MGAS11113
            SAMN08555799-rid19682583.denovo.043, whole genome shotgun sequence.
ACCESSION   DATXIG010000043 DATXIG010000000
VERSION     DATXIG010000043.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN08555799
            Sequence Read Archive: SRR6740495
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pyogenes
  ORGANISM  Streptococcus pyogenes
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 11431)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11431)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-OCT-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-OCT-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001918058.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001918058.2
            Genome Coverage       :: 368x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/12/2023 11:23:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 1,806
            CDSs (total)                      :: 1,769
            Genes (coding)                    :: 1,722
            CDSs (with protein)               :: 1,722
            Genes (RNA)                       :: 37
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1, 1 (5S, 23S)
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 47
            CDSs (without protein)            :: 47
            Pseudo Genes (ambiguous residues) :: 0 of 47
            Pseudo Genes (frameshifted)       :: 30 of 47
            Pseudo Genes (incomplete)         :: 20 of 47
            Pseudo Genes (internal stop)      :: 9 of 47
            Pseudo Genes (multiple problems)  :: 11 of 47
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11431
                     /organism="Streptococcus pyogenes"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN08555799-rid19682583.denovo.043"
                     /strain="MGAS11113"
                     /serotype="Emm28"
                     /isolation_source="normally sterile site"
                     /host="Homo sapiens"
                     /db_xref="taxon:1314"
                     /country="Finland"
                     /collection_date="2002"
                     /collected_by="National Public Health Laboratory"
     gene            complement(97..951)
                     /locus_tag="VC122_001279"
     CDS             complement(97..951)
                     /locus_tag="VC122_001279"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002988161.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5'-nucleotidase, lipoprotein e(P4) family"
                     /protein_id="HEP5641203.1"
                     /translation="MKSKKVVSVISLTLSLFLVIGCAKVDNNKSVNCKPATKQTYNSY
                     SDDQLRSRENTMSVLWYQRAAETQALYLQGYQLATDRLKEQLNKPTDKPYSIVLDIDE
                     TVLDNSPYQAKNVLEGTGFTPESWDYWVQKKEAKPVAGAKDFLQFADQNGVQIYYISD
                     RSTTQVDATMENLQKEGIPVQGRDHLLFLEKGVKSKESRRQKVKETTNVTMLFGDNLL
                     DFADFSKKSQEDRTALLSDLQEEFGRRFIIFPNPMYGSWEGAIYKGEKLDVLKQLEER
                     RKSLKSFK"
     gene            complement(1181..2071)
                     /locus_tag="VC122_001280"
     CDS             complement(1181..2071)
                     /locus_tag="VC122_001280"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002982880.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SPFH domain-containing protein"
                     /protein_id="HEP5641204.1"
                     /translation="MLGPFIFIAFGVIVILAIVASTLYVVRQQSVAIVERFGRYQKTA
                     TSGIHVRLPFGIDKIAARVQLRLLQSEIIVETKTKDNVFVTLNVATQYRVNEQNVTDA
                     YYKLMKPESQIKSYIEDALRSSVPKLTLDELFEKKDEIALEVQHQVAEEMSTYGYIIV
                     KTLITKVEPDAEVKQSMNEINAAQRKRVAAQELANADKIKIVTAAEAEAEKDRLHGVG
                     IAQQRKAIVDGLAESIQELKEANISLNEEQIMSILLTNQYLDTLNTFATKGNQTLFLP
                     NTPSGVEDIRTQVLSALKTK"
     gene            complement(2308..3972)
                     /locus_tag="VC122_001281"
     CDS             complement(2308..3972)
                     /locus_tag="VC122_001281"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000036699.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DAK2 domain-containing protein"
                     /protein_id="HEP5641205.1"
                     /translation="MSNITTSLFQEMVQAAATRLGKQAEYVNSLNVFPVPDGDTGTNM
                     SMTMDNGAKEVADKPASTVGEVGQMLSKGLLMGARGNSGVITSQLFRGFGQSIKGKDE
                     LTGKDLAQAFQVGVEVAYKAVMKPVEGTILTVSRGAATAALKKADLTDDAVEVMQAAL
                     DGAKGALAKTPDLLPVLKEVGVVDSGGQGLVFIYEGFLSALNGDYVTSADFKATPANM
                     SEMINAEHHKSVVGHVATEDITYGYCTEIMVALKQGPTYVKEFNYDEFQGYLSGLGDS
                     LLVVNDDEIVKVHVHTEDPGLVMQEGLKYGSLIKIKVDNMRNQHEAQVQKTDVEKNKA
                     EVKEFGLIAVVAGEGLSEIFKAQGVDYVISGGQTMNPSTEDIVKAIEAVNAKQVIILP
                     NNKNIFMAAQSAAEVVDIPAAVVATRTVPQGFTSLLAFDPSKSLEDNVADMSTSLSDV
                     VSGSVTLAVRDTTIDGLEIHENDFLGMVDGKIIVSDPDMEATLKAAFEKMIDEDSEIV
                     TIFVGEEGDQDLAEELAGYLGETYEDVEVEIHQGDQPVYPYLMSVE"
     gene            complement(3972..4337)
                     /locus_tag="VC122_001282"
     CDS             complement(3972..4337)
                     /locus_tag="VC122_001282"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018365170.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Asp23/Gls24 family envelope stress response
                     protein"
                     /protein_id="HEP5641206.1"
                     /translation="MTVKINTKDGLIELSDDVIATVVGGSATEIFGVVGMASKSAIKD
                     NFQSLLRKENYAKGVVVKSTDLGISVDVYTVMSYGVKISEVSKNIQERVKFNLESQLG
                     LTADMVNVYVQNIKVVGEN"
     gene            complement(4489..4677)
                     /locus_tag="VC122_001283"
     CDS             complement(4489..4677)
                     /locus_tag="VC122_001283"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008536053.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L28"
                     /protein_id="HEP5641207.1"
                     /translation="MAKVCYFTGRKTVSGNNRSHAMNQTKRTVKPNLQKVTILVDGKP
                     KKVWASARALKSGKVERI"
     gene            complement(5059..5940)
                     /locus_tag="VC122_001284"
     CDS             complement(5059..5940)
                     /locus_tag="VC122_001284"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011184946.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fructose-bisphosphate aldolase"
                     /protein_id="HEP5641208.1"
                     /translation="MAIVSAEKFVQAARENGYAVGGFNTNNLEWTQAILRAAEAKQAP
                     VLIQTSMGAAKYMGGYKVCQSLITNLVESMGITVPVAIHLDHGHYEDALECIEVGYTS
                     IMFDGSHLPVEENLAKTAEVVKIAHAKGVSVEAEVGTIGGEEDGIIGKGELAPIEDAK
                     AMVETGIDFLAAGIGNIHGPYPENWEGLALDHLEKLTAAVPGFPIVLHGGSGIPDDQI
                     KEAIRLGVAKVNVNTESQIAFSNATREFARNYEANEAEYDGKKLFDPRKFLAPGMKAV
                     QGAVEERIDVFGSANKA"
     gene            complement(6147..6232)
                     /locus_tag="VC122_001285"
     tRNA            complement(6147..6232)
                     /locus_tag="VC122_001285"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(6196..6198),aa:Leu,seq:aag)
     gene            complement(6286..7212)
                     /locus_tag="VC122_001286"
     CDS             complement(6286..7212)
                     /locus_tag="VC122_001286"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002988151.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HEP5641209.1"
                     /translation="MKTIRIAKYLGILFLLITLISVGASFYFFHVAQIREEKSFINNK
                     KRSTNNPLYPAEQSFDALPYEKRQLTNRGLKQVGWYLPAAQKTKKTAIVVHGFTNDKE
                     DMKPYAMLFHDLGYNVLMPDNEAHGESEGNLIGYGWNDRLNVMAWTDQLIKENPESQI
                     TLFGLSMGAATVMMASGERLPAQVTSLIEDCGYTSVWDELKFQAKAMYNLPAFPLLYE
                     VSALSKIRAGFSYGEASSVKQLAKNKRPTLFIHGDKDDFVPTKMVYDNYKATKGPKEI
                     LIVKGAKHAKSFETNPEQYQKKIAAFLKKVEK"
     gene            complement(7380..8984)
                     /locus_tag="VC122_001287"
     CDS             complement(7380..8984)
                     /locus_tag="VC122_001287"
                     /EC_number="6.3.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002982843.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CTP synthase"
                     /protein_id="HEP5641210.1"
                     /translation="MTKYIFVTGGVVSSIGKGIVAASLGRLLKNRGLKVTIQKFDPYI
                     NIDPGTMSPYQHGEVYVTDDGAETDLDLGHYERFIDINLNKYSNVTTGKIYSEVLRKE
                     RKGEYLGATVQVIPHITDALKEKIKRAASTTDSDVIITEVGGTVGDIESLPFLEALRQ
                     MKADVGSENVMYIHTTLLPYLKAAGEMKTKPTQHSVKELRGLGIQPNMLVIRTEEPVE
                     QGIKNKLAQFCDVNSEAVIESRNVEHLYQIPLNLQAQSMDQIVCDHLKLNAPQADMTE
                     WSAMVDKVMNLRKTTKIALVGKYVELPDAYLSVVEALKHSGYANDTAIDLKWVNANDV
                     TVDNAADLLGDADGIIVPGGFGQRGTEGKIQAIRYARENDVPMLGICLGMQLTCVEFA
                     RHVLNMEGANSFELEPSTKYPIIDIMRDQIDIEDMGGTLRLGLYPCKLKPGSKAAMAY
                     NNQEVVQRRHRHRYEFNNKFRPEFEAAGFVFSGVSPDNRLVEIVELKEKKFFVAAQYH
                     PELQSRPNRPEELYTAFVTAAIKNSN"
     gene            complement(9241..9816)
                     /locus_tag="VC122_001288"
     CDS             complement(9241..9816)
                     /locus_tag="VC122_001288"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002988147.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit delta"
                     /protein_id="HEP5641211.1"
                     /translation="MKLDVFAGQEKSELSMIEVARAILEERGRDNEMYFSDLVNEIQN
                     YLGKSDAGIRHALPFFYTDLNTDGSFIPLGENKWGLRSWYAIDEIDEEIITLEEDEDG
                     AQKRKKKRVNAFMDGDEDAIDYRDDDPEDEDFTEESAEVEYDEEDPDDEKSEVESYDS
                     ELNEIIPEDDFEEVDINEEDEEDEEDEEPVL"
     gene            complement(10028..11311)
                     /locus_tag="VC122_001289"
     CDS             complement(10028..11311)
                     /locus_tag="VC122_001289"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017645628.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="trigger factor"
                     /protein_id="HEP5641212.1"
                     /translation="MSTSFENKATNRGVITFTISQDKIKPALDKAFNKIKKDLNAPGF
                     RKGHMPRPVFNQKFGEEVLYEDALNIVLPEAYEAAVTELGLDVVAQPKIDVVSMEKGK
                     EWTLSAEVVTKPEVKLGDYKNLVVEVDASKEVSDEDVDAKIERERQNLAELIIKDGEA
                     AQGDTVVIDFVGSVDGVEFDGGKGDNFSLELGSGQFIPGFEDQLVGAKAGDEVEVNVT
                     FPESYQAEDLAGKAAKFMTTIHEVKTKEVPELDDELAKDIDEDVDTLEDLKVKYRKEL
                     EAAQETAYDDAVEGAAIELAVANAEIVDLPEEMIHEEVNRSVNEFMGNMQRQGISPEM
                     YFQLTGTTQEDLHNQYSAEADKRVKTNLVIEAIAKAEGFEATDSEIEQEINDLATEYN
                     MPADQVRSLLSADMLKHDIAMKKAVEVITSTASVK"
BASE COUNT         3297 a         2467 c         1872 g         3795 t
ORIGIN      
        1 aaatcaacat ttttttcagt cattaaccct attttcagaa aataatagta cttaaaaaga
       61 ggttacattt tcttttgcac ctctttttca attactttat ttaaagcttt ttaaactttt
      121 acggcgttcc tctagttgct taagcacatc cagcttttca cctttataaa tggcaccttc
      181 ccatgaacca tacataggat tagggaaaat gataaagcgt cttccaaact cttcttgcaa
      241 atctgataat aaagctgttc tatcttcttg agattttttt gaaaaatcag caaaatctag
      301 aagattatca ccaaatagca tcgttacatt agttgtttct ttgacctttt gacgacgact
      361 ctcctttgat tttacgcctt tttctaagaa tagaagatga tcacgacctt gtactggaat
      421 accttctttt tggagatttt ccattgtagc atctacttga gtagttgatc tgtctgaaat
      481 gtagtaaatt tgaacaccat tttggtctgc aaattgcaaa aagtctttag caccagcaac
      541 cggttttgct tctttctttt gtacccaata atcccagctt tcaggtgtaa atcctgttcc
      601 ttccaaaaca tttttagctt gataagggct attatcaaga actgtttcgt caatatctaa
      661 tacaattgaa taaggtttat ccgttggttt attgagttgt tcttttaagc gatccgttgc
      721 taactgatac ccctgtaaat aaagcgcttg agtttccgca gctcgctggt accataaaac
      781 agacatggta ttttcacgcg atcttaattg gtcatcacta tagctattat aggtttgttt
      841 agtagcaggc ttacaattaa ctgatttgtt gttatcaact ttagcacaac ctatcaccaa
      901 aaaaagggat aaggtaagtg atataacact aacaactttt ttggatttca ttgaaaatct
      961 cctttttgct tattaagttg attttaaact tatttaattt aaaagtcaat acaacttaac
     1021 atttaatcaa tactatgttg ttaatcatga aaaaggcttt ttttactttc atttctccaa
     1081 taaaagtaat gtattcattt cgtcgtttgc cacttcacaa ggtatgatag ccttctgcta
     1141 aatccagtat tgagcaactt tttagccaag cttttacacg ttattttgtt ttaagggcag
     1201 ataaaacttg tgtacggata tcctctaccc cacttggtgt atttggtaga aaaagggttt
     1261 ggtttccttt tgtcgcaaag gtattgaggg tatccaggta ttggttggtc aacaaaatgg
     1321 acatgatttg ttcttcgttt aaggaaatat tcgcttcttt aagctcttga atagactccg
     1381 ccaaaccatc cacaatggcc ttacgctgtt gggcaatccc taccccgtgc aatcgatctt
     1441 tttcagcctc tgcttctgct gctgtcacaa ttttaatttt atctgcgttt gccaactctt
     1501 gagcagctac acgtttgcgt tgagccgcat tgatttcatt cattgactgt ttgacttcag
     1561 catcaggctc caccttggta atcaaggttt tgacaataat atatccatag gttgacattt
     1621 cttctgccac ttgatgttgt acttccaagg caatttcatc ttttttctca aacaattcgt
     1681 ctaaggtcaa cttcggcacg gatgaccgca aggcatcttc aatataagac ttgatttgag
     1741 attcaggttt cattaatttg tagtaagcat ctgttacatt ttgttcgtta acacgatact
     1801 gagtggcaac attcaaagtt acaaacacat tgtccttggt tttggtttca acgatgattt
     1861 ctgactgtaa aagacgcagc tgcacacgcg cagcaatttt gtcaatccca aacggaaggc
     1921 ggacatgaat accactcgtg gctgtttttt ggtacctacc aaaacgttcc acgatggcaa
     1981 cagattgttg acgaacaaca taaagggtac tggcaacaat agctaggatt acaataaccc
     2041 caaaagcaat aaagataaat ggtcctaaca taaaatctcc tccttgtcat atcttagaac
     2101 tgaaccgttt tcagttcttg acttattgtt ataatacaat tataatgttt ttctaggttt
     2161 tgtcaaacta aatcgtaacc attagctttg tgatggatta ccctcatttt ttaggggctt
     2221 accatcacta aaaccactca ttacgacatc aatatagaca aaaagagtca gacctttgcc
     2281 taactcctct ttttttattg tattctatta ttctacactc atgagatatg ggtaaacagg
     2341 ctgatctcct tgatggatct caacttccac atcttcgtaa gtctctccta aatacccagc
     2401 aagttcttct gctaagtctt gatcaccttc ttcaccaaca aagatagtca caatctcgct
     2461 atcttcgtca atcatctttt caaaggcagc ttttagagtt gcttccatgt ctgggtctga
     2521 aacgatgatt ttaccatcaa ccattcccaa gaaatcattt tcatggattt ctaaaccatc
     2581 aatggtggtg tcacggactg ccaaagtaac gcttccactg accacgtcag aaagactagt
     2641 tgacatatct gctacattgt cctctaaaga ttttgaagga tcaaaggcca acaagctggt
     2701 aaatccttgc ggaacagtac gtgttgcaac aacagcagcc gggatatcca ccacttctgc
     2761 agcagattgg gcagccataa agatattttt attgtttggc aagataatga cttgtttggc
     2821 attaactgct tcaatagctt taacaatatc ttctgtagat ggattcatgg tttgaccacc
     2881 agaaatcacg taatcaacac cttgcgcctt gaaaatttca gataaacctt ctccagctac
     2941 tactgcaatc aagccaaatt cttttacttc agccttgttt ttttcgacat cagtcttttg
     3001 aacctgtgct tcatgctggt tacgcatatt gtccacttta atcttgatga gcgaaccata
     3061 tttaagacct tcttgcatca caagtccggg gtcttcagta tggacgtgaa ctttaacaat
     3121 ctcatcatca ttgaccacta acagcgagtc accaaggcca ctaaggtagc cttgaaactc
     3181 atcatagttg aattctttca cataggttgg gccttgttta agggcaacca tgatttcagt
     3241 acaataaccg taggtaatgt cttcagtagc cacatgacca acaacggatt tgtgatgctc
     3301 agcattaatc atttcagaca tgttagcagg agtcgcttta aagtctgctg aagtcacata
     3361 gtcgccattc aaagcagata ggaaaccttc atagataaag acaagacctt gaccgcctga
     3421 atcaaccaca ccaacttctt taaggactgg taataaatca ggtgtcttag caagggcacc
     3481 tttggcaccg tctaaagcag cttgcatgac ttccacggcg tcgtctgtaa gatccgcttt
     3541 ttttaaggca gcggttgctg ctccacgaga tactgtcaag attgtccctt caacaggttt
     3601 catcactgct ttgtaggcaa cttctacacc cacttgaaag gcttgtgcca aatctttacc
     3661 tgtcagctca tcttttccct taatactttg accaaaacca cgaaaaagtt gtgaagtaat
     3721 aactccagaa tttccacgcg ctcccatcaa aagacctttt gataacattt gtccaacttc
     3781 tcccacagtt gacgcaggtt tatcagcaac ttccttggca ccattgtcca tagtcatact
     3841 catgtttgtt cctgtatcac catctggaac aggaaaaaca tttaaggaat taacatattc
     3901 tgcttgtttg ccaaggcgag tcgctgccgc ttggaccatt tcttggaata agcttgttgt
     3961 aatatttgac actaattttc tcctacaacc ttaatatttt gcacgtaaac attgaccata
     4021 tcagcagtga gaccaagctg actttcaagg ttaaatttca cgcgttcttg gatattttta
     4081 gagacttcac tgattttcac accataactc atcacagtgt agacatcaac agaaataccc
     4141 agatctgttg attttactac aacaccctta gcgtagtttt ctttgcgaag tagtgattga
     4201 aaattgtctt ttattgcact tttgctggcc attccaacaa caccaaaaat ttccgttgct
     4261 gatcctccca cgacagttgc aatcacatcg tcggatagct cgattagacc atcttttgta
     4321 ttaattttta cagtcatagt tactacctca aaaagttttt atcatcctat tttatcatat
     4381 tttagaagta atgtaaaaga gatagaatag ttaacgatag cgtttattac cctgactagc
     4441 ttatggatag aaataagcta ataaaaaaag ccctaaggct tatttctatt agatgcgttc
     4501 tactttacca gatttaagcg cacgagcaga agcccaaact tttttaggtt taccatcaac
     4561 aaggatcgta actttttgaa ggtttggttt aactgtacgt ttcgtttggt tcatcgcgtg
     4621 tgaacggttg tttccagaaa cggttttacg tcctgtaaaa taacatactt tagccatttt
     4681 gatctgtttc ctcctacgta gaatatgttt acggatgtgc tagcaccaca tacctatcta
     4741 ttctatcata gggtgcttaa tttgacaagg tatttcccat tttctttttc aacaatttta
     4801 cggaattact attacaatag tctacgtact catttttcac tacatttttt agctaaaaca
     4861 tattaacatt ccgcttaacc tctttaacaa taacaaaaag acttaccata gatagcaggg
     4921 tccagatttt aatactctaa attatgcttt gtttgttcac aatcaaagat tgtttcacag
     4981 aaactggtat ccattttagg gtgtcaattg tatctaaaaa agccctcaag agggctttag
     5041 ggtatttaat actctaaatt atgctttgtt agctgatccg aaaacatcga tacgttcttc
     5101 aacagcacct tgaacagctt tcatacctgg agccaagaat ttacgtgggt caaataattt
     5161 tttaccatca tattctgctt cgttagcttc gtagttacga gcaaattcac gagttgcgtt
     5221 agagaaagcg atttgacttt cagtgttaac gttaacttta gcaacaccaa gacggattgc
     5281 ttctttgatt tgatcgtcag gaataccaga accaccgtgc aatacgattg ggaaacctgg
     5341 tacagctgct gttaattttt ctaagtggtc aagagcaaga ccttcccagt tttctgggta
     5401 tggaccgtgg atgttaccga taccagctgc caagaagtca attccagttt caaccattgc
     5461 tttagcatct tcgattggag cgagctcacc tttaccgatg ataccgtctt cttcaccacc
     5521 gattgtacca acttcagctt caactgaaac gcctttagca tgtgcaattt taacaacttc
     5581 tgcagtttta gcaaggtttt cttcaactgg aaggtgtgaa ccgtcaaaca tgattgaagt
     5641 gtaacctact tcgatacact caagagcatc ttcgtagtga ccatgatcaa ggtggatagc
     5701 aacaggaaca gtgataccca ttgactcaac aaggttagta ataagagatt gacatacttt
     5761 ataaccaccc atgtattttg ctgcacccat tgatgtttgg ataagaactg gagcttgttt
     5821 agcttctgct gcacgcaaga ttgcttgagt ccactctaag ttgtttgtgt taaatccacc
     5881 aacagcatat ccgttttcac gagctgcttg gacaaatttt tctgctgaaa cgattgccat
     5941 ttgtaaggcc tcctcttatt tttcgggata actccccgtt tacattgttc attctagcac
     6001 aattttccat aaatttctag tctttcacaa gcttaaagcg ttttccaaaa gcagattatc
     6061 aaaagttttc tccagtaaat ctatgccttc ttatccttca tttaaccaaa aaaaagagac
     6121 gaatcaatga ttcacctctt acattatgcg gagagtggga cttgaaccca cacgacctaa
     6181 agcggtcaca ggatccttag tcctgcgcgt ctgccaattc cgccatcccc gctactcatc
     6241 agtaacataa attattatag caactgtgag tttaactgtc aatacttatt tctcaacttt
     6301 tttcaaaaaa gcggcaattt ttttctggta ttgttctggg tttgtttcaa aggatttggc
     6361 gtgttttgcc cctttaacaa tcaagatttc cttaggacct ttcgtggcct tataattgtc
     6421 ataaaccatt tttgtaggaa caaaatcatc cttatcaccg tggataaata aagttggacg
     6481 tttattttta gccagctgtt tcactgagct cgcttctccg taactaaaac ctgctcgaat
     6541 cttagataag gcagagactt catagagtaa aggaaaggca ggcaagttgt acatagcctt
     6601 ggcctgaaac ttcaattcgt cccaaacact ggtataaccg caatcttcga tgagggaggt
     6661 gacttgcgca ggcaatcgct caccacttgc catcattact gttgcagcac ccatagataa
     6721 gccaaagagt gtgatttggc tttcagggtt ttccttaatc agttggtctg tccaagccat
     6781 gacattaagg cggtcattcc agccataacc aatcaagttc ccttcacttt ccccatgggc
     6841 ctcattgtct ggcattaaga cattatagcc caaatcatga aaaagcatgg catatggctt
     6901 catatcttct ttgtcattcg taaaaccatg aacaacaata gctgtctttt ttgttttttg
     6961 agcagctggt aagtaccacc ccacttgttt taacccacga tttgttagtt gacgtttttc
     7021 gtaaggtaaa gcgtcaaaag actgttcagc tgggtataat ggattatttg tactccgttt
     7081 cttattatta ataaacgatt tctcttctct tatttgtgca acatgaaaga aataaaaact
     7141 agcacctacg ctaataagag ttatcaaaag gaatagaatt cctaaatact ttgctattcg
     7201 aatagttttc ataccaagta ttttacaaaa aaatgactga aaaagaaagt gctaacctaa
     7261 aaatatcacg tttttctttt tctaatgcta tttttataga aaagatccct ctaatagcta
     7321 gtaatcttta tcagtatctg ccagccaagt agaatgccac aaacttaccc ttaaaaccac
     7381 taattgctat ttttaatagc agctgtaaca aaagcggtgt atagctcctc aggacgattt
     7441 ggacggcttt gcaattctgg gtggtattga gctgctacaa agaatttttt ctctttcaat
     7501 tctacaattt caactaagcg gttgtcaggt gatacccctg agaaaacaaa accagctgct
     7561 tcaaattctg gacggaattt gttgttgaac tcataacggt gacgatgacg acgttgcacc
     7621 acctcttgat tattgtaagc cattgctgct tttgaacctg gtttcaattt acatgggtac
     7681 aatcccagac gaagcgtacc tcccatatct tcaatatcaa tttgatcacg catgatatca
     7741 atgatggggt atttagtact tggctctaat tcaaaagagt ttgcaccttc catattgagg
     7801 acatggcgag caaattcaac acaagtcagt tgcatgccaa gacagatacc aagcattggc
     7861 acatcattct cgcgggcata gcgaatcgct tggatcttgc cttctgtacc acgttgacca
     7921 aaaccacctg gtacaataat cccatccgca tctcccaaca agtcagctgc attatccacc
     7981 gtcacatcgt tagcattgac ccattttagg tcaattgcgg tatcattggc ataaccagaa
     8041 tgtttcaagg cttccacaac agataagtag gcatctggca attccacata tttaccaacc
     8101 aaggcaattt ttgttgtttt tcttaaattc atcaccttgt caaccatagc tgaccactca
     8161 gtcatatcag cttgtggcgc attcaatttc aaatgatcac aaacaatctg atccattgat
     8221 tgggcttgca agttcaatgg aatttgatac aagtgttcta cattacgtga ctcgataaca
     8281 gcttctgaat tcacatcaca gaactgagcc aatttatttt taatcccctg ctcaactggc
     8341 tcttccgtac gaataaccaa catattcggt tgaatcccta aaccacgcaa ttctttaacg
     8401 gaatgttgcg ttggcttagt tttcatttca cccgctgcct ttaaatatgg caacaaggtg
     8461 gtgtgaatat acatcacatt ttcagaacca acatcagctt tcatctgacg aagagcttct
     8521 aagaacggta aactttcaat atcgccaaca gtccccccga cctcagtaat aattacatca
     8581 gagtccgttg ttgaagctgc gcgtttgatt ttttctttaa gggcatctgt gatatgagga
     8641 atcacctgaa cagtcgctcc aaggtactca cctttacgtt ctttacgaag gacttcacta
     8701 taaattttac ctgttgtaac attggaatat ttattaagat tgatatcaat aaaacgttca
     8761 tagtgaccca aatccaagtc ggtttcagca ccgtcatctg tcacatatac ttctccatgc
     8821 tgataggggc tcatcgtccc tggatcaata ttaatataag gatcaaattt ttgaatcgtc
     8881 accttaagtc cacgattttt taacaatcgg ccaagacttg ccgcaacaat tcctttgcca
     8941 attgatgata caacaccacc agttacaaaa atgtatttcg tcatagtact cctttatata
     9001 agctgaataa aagcattgct ttcttgttaa ttccctatta gttggttcca cattggttag
     9061 gtatcacctt agttttctaa atatagaggc aaactggcat tattctagaa aacaaaaata
     9121 gccccctatt aactagagag cttttgcctg acctcataaa gaggtgcccg aataataata
     9181 taaactataa tagacaattt gtcaaatgat aatcacattt ttatttctga actaaagaca
     9241 ttagagaact ggttcttcat cttcttcatc ttcttcgtct tcttcattga tatcaacttc
     9301 ttcaaaatca tcttcaggga taatttcatt caattctgaa tcatatgact ccacttctga
     9361 tttttcatca tctggatctt cttcatcata ttcaacctca gctgattctt ctgtgaaatc
     9421 ttcatcttct ggatcatcat ctctataatc aatagcgtct tcatctccat ccatgaaagc
     9481 attaacgcgt tttttcttac gtttttgtgc gccatcttca tcttcttcta gagtaataat
     9541 ttcttcatca atttcatcaa tagcatacca cgaacgaagt ccccatttgt tttcacctag
     9601 aggaataaat gagccatccg tatttaagtc agtatagaaa aatggtaatg catgacgaat
     9661 acctgcatca gattttccta agtaattttg aatctcattg actaaatcgc taaagtacat
     9721 ctcattgtcg cgaccgcgtt cttccaaaat ggcacgcgcc acttcaatca tggaaagctc
     9781 gcttttttct tgtcctgcaa atacgtctaa tttcaaggca gttctcctta ttttatcatt
     9841 ctctaccatt ttacgctaaa aaaagtaatt cgtcaaaact tttatcaact catatcactt
     9901 cttcttgaca aatctttaaa gctaattcat taaaataacg tttaactaat tccttatctt
     9961 tagaaaaaga aaaaccagac tagaaagacg ttctaatccg gcttgtacgt tttgttcctt
    10021 aataagatta cttaacgctt gctgtgcttg taatcacttc aactgctttt ttcattgcaa
    10081 tatcatgttt caacatatct gctgaaagaa gagaacgaac ttggtcagct ggcatgttat
    10141 attctgttgc aaggtcatta atttcttgtt caatttcact atctgttgct tcaaaacctt
    10201 ctgctttagc aattgcttca ataacaaggt ttgttttaac acgcttgtca gcttcagctg
    10261 aatattggtt atgtaaatct tcttgagttg taccagtcaa ttggaagtac atttcaggtg
    10321 agattccttg acgttgcatg ttgcccataa attcattcac tgaacggttg acttcttcat
    10381 gaatcatttc ttcgggtaaa tcaacaattt cagcatttgc aactgctaat tcaatcgcag
    10441 ctccttcaac agcatcatca taagcagttt cttgagctgc ttcaagttct ttacgatatt
    10501 ttacttttaa gtcttcaagt gtgtcaacat cttcatcaat atcttttgca agctcatcat
    10561 caagctctgg tacttctttt gttttgactt cgtgaatagt tgtcataaat ttagcggctt
    10621 tacctgcaag atcttctgct tggtaagatt ctgggaatgt gacattaact tctacttcat
    10681 cgccagcttt agcaccaact agttgatctt caaaacctgg gataaattgt cctgaaccaa
    10741 gttcaagaga gaagttatct cctttaccgc catcaaactc aacaccatca actgaaccaa
    10801 caaagtcaat cacaacagtg tcaccttgag ctgcttcacc gtctttaata atgagttccg
    10861 caaggttttg acgttcgcgc tcaattttag catccacgtc ttcatctgaa acttcttttg
    10921 aagcatcaac ttctacaaca aggtttttgt aatcaccaag tttcacttca ggttttgtca
    10981 caacttcagc agaaagtgtc cactctttcc ctttttccat tgacacaaca tcgatttttg
    11041 gttgtgcaac cacatcaaga ccaagttccg tcacagctgc ttcataagct tctggcaata
    11101 caatattcaa agcatcttca taaagaactt cttcaccaaa tttttggttg aagactggac
    11161 gaggcatgtg tcctttacgg aaacctggtg catttaagtc ttttttgatt ttattaaaag
    11221 ctttatcaag agctggtttt attttatctt gactaattgt aaatgtaatc acgccacgat
    11281 ttgtagcttt gttttcaaat gatgtagaca ttaagtcatt tctccttaaa ataatttcga
    11341 tacagttcat tttatcataa tttaaagcgt ttttaaagct ttttatgcgt gaaatcaaac
    11401 gcgctattaa gagtatctac ctgtcattag t
//
LOCUS       DATXIG010000044        11058 bp    DNA     linear   BCT 28-DEC-2023
DEFINITION  TPA_asm: Streptococcus pyogenes strain MGAS11113
            SAMN08555799-rid19682583.denovo.044, whole genome shotgun sequence.
ACCESSION   DATXIG010000044 DATXIG010000000
VERSION     DATXIG010000044.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN08555799
            Sequence Read Archive: SRR6740495
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pyogenes
  ORGANISM  Streptococcus pyogenes
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 11058)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11058)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-OCT-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-OCT-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001918058.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001918058.2
            Genome Coverage       :: 368x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/12/2023 11:23:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 1,806
            CDSs (total)                      :: 1,769
            Genes (coding)                    :: 1,722
            CDSs (with protein)               :: 1,722
            Genes (RNA)                       :: 37
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1, 1 (5S, 23S)
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 47
            CDSs (without protein)            :: 47
            Pseudo Genes (ambiguous residues) :: 0 of 47
            Pseudo Genes (frameshifted)       :: 30 of 47
            Pseudo Genes (incomplete)         :: 20 of 47
            Pseudo Genes (internal stop)      :: 9 of 47
            Pseudo Genes (multiple problems)  :: 11 of 47
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11058
                     /organism="Streptococcus pyogenes"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN08555799-rid19682583.denovo.044"
                     /strain="MGAS11113"
                     /serotype="Emm28"
                     /isolation_source="normally sterile site"
                     /host="Homo sapiens"
                     /db_xref="taxon:1314"
                     /country="Finland"
                     /collection_date="2002"
                     /collected_by="National Public Health Laboratory"
     gene            complement(121..1647)
                     /locus_tag="VC122_001290"
     CDS             complement(121..1647)
                     /locus_tag="VC122_001290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014411840.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease/substrate-binding
                     protein"
                     /protein_id="HEP5641213.1"
                     /translation="MPSLFVTFQNRFNEWLAALGEHLQISLLSLMIALLIGVPLAALL
                     SRSKRWSDIMLQVTGVFQTIPSLALLGLFIPLMGIGTLPAVTALVIYAIFPILQNTIT
                     GLNGIDPSLVEAGIAFGMTKWERLKTFEIPIAMPVIMSGVRTSAVMIIGTATLASLIG
                     AGGLGSFILLGIDRNNANLILIGAISSALLAIIFNSLLQYLEKASLRRIMISFGITLL
                     ALLASYTPMALSQFSKGKDTVVIAGKLGAEPDILINLYKELIEDQSDISVELKSNFGK
                     TSFLYEALKSGDIDIYPEFTGTITSSLLRDKPPLSNDPKQVYEDAKKGIAKQDKLTLL
                     KPFAYQNTYAVAMPEKLAKEYQIETISDLKAHADTLKAGFTLEFKDRADGYKGMQSQY
                     GLQLSVATMEPALRYQAIQSGDIQVTDAYSTDAEITKYHLKVLKDDKQLFPPYQGAPL
                     MKTSLLTKHPELKGILNQLAGKITEKEMQDMNYEVSVKGADANKVARDYLLKTGLIQK
                     "
     gene            complement(1640..2368)
                     /locus_tag="VC122_001291"
     CDS             complement(1640..2368)
                     /locus_tag="VC122_001291"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010922297.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEP5641214.1"
                     /translation="MIRFNNVSKTFGQTKVLQEQTFQINDREFFVLVGPSGSGKTTLL
                     KMINCLIEPSSGDILLNNVPQKELDLREMRLSIGYVLQQIALFPNLTVAENIAIIPEM
                     KQWSAEEIRQKTEELLDKVGLPAKDYLDRYPSDLSGGEQQRIGIVRAIISHPKILLMD
                     EPFSALDPISRKQLQELMLSLHKEFDMTIVFVTHDIDEAIKLGDRVAILNEGEIVQLD
                     RPEMIKTHPANAFVANLFGGDEHA"
     gene            2791..3480
                     /locus_tag="VC122_001292"
     CDS             2791..3480
                     /locus_tag="VC122_001292"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011184502.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug transporter"
                     /protein_id="HEP5641215.1"
                     /translation="MKNAISILALLTSENQLEDMFIRELSHHFSHLFEVTILTSKANI
                     ESNQLSTFQGIFSFHEHDIDLPTLYFKTSQHGQGFLVTESVFDQATAVLSLSQYLTRF
                     YQKFDRHFLQYLPLQARLSDANGNIIVDNHAFNGSFLPTTDKEIEDWILAELRLSDNP
                     CKTFLLPSGSLDHIYMQHYQALKNPQGQLVGVLDTVQDIKPLLNQYLEETGQAIVGWS
                     DVTSGPSILDH"
     gene            complement(3674..4429)
                     /locus_tag="VC122_001293"
     CDS             complement(3674..4429)
                     /locus_tag="VC122_001293"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014407568.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family NAD(P)-dependent oxidoreductase"
                     /protein_id="HEP5641216.1"
                     /translation="MGNIALVTGASAGFGRAIVEQLIADGIKVIGASRRLEKLKALET
                     SFGSDNFYPLQMDVTDTKAIDDALTSLPNQWQDITILVNNAGLALGLDKAYEADFENW
                     MTMINTNIVGLIYLTRQLLPHMVSKDDGIIINLGSTAGTIPYPGANIYGASKAFVKQF
                     SLNLRADLAGTHIRVTNIEPGLCDGTEFSKVRFNGDEARVKAIYQGAHAIQPQDIAST
                     VSWVIHQPPHVNVNRIELMPVSQSYGPQPVSRD"
     gene            complement(4556..5551)
                     /gene="pta"
                     /locus_tag="VC122_001294"
     CDS             complement(4556..5551)
                     /gene="pta"
                     /locus_tag="VC122_001294"
                     /EC_number="2.3.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011184500.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate acetyltransferase"
                     /protein_id="HEP5641217.1"
                     /translation="MSIRSLFGGLREKILGKNMKIVFPEGNDERVVRAAARLKFEGLL
                     EPIILGQSEEVRNLLTKLGFADQDYTIINPNEYADFDKMKEAFVDIRKGKATLEDADK
                     MLRDVNYFGVMLVKMGLADGMVSGAIHSTADTVRPALQIIKTKPGISRTSGVFLMNRE
                     NTSERYVFADCAINIDPTAQELAEIAVNTAETAKIFDIDPKIAMLSFSTKGSGKAPQV
                     DKVREATEIAKGLNPDLALDGELQFDAAFVPETAAIKAPDSAVAGQANTFIFPDLQSG
                     NIGYKIAQRLGMFDAIGPILQGLNKPVNDLSRGSSAEDIYKLAIITAAQAIESQG"
     gene            complement(5555..6460)
                     /locus_tag="VC122_001295"
     CDS             complement(5555..6460)
                     /locus_tag="VC122_001295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003054007.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RluA family pseudouridine synthase"
                     /protein_id="HEP5641218.1"
                     /translation="MRFEFVADKRIKVKTLLKSHDVSKGLLAKIKYKGGNILVNGIEQ
                     NAIYLLQVGDVVTIDIPNEEPFEKLEAIPFDLDIVHEDDHFLVINKPIGFASIPSAIH
                     SNTIANFIKAYYVDNHYLDQQVHIVTRLDRDTSGLMLFAKHGYAHARLDKQLQTRSIE
                     KRYFALVSGNGTLPDEGDIIAPIGRSKDSIITRAVDPMGKYAKTSYKVVARYSENVHL
                     VDIKLHTGRTHQIRVHFAHIGFPLLGDDLYGGRLDLGITRQALHCHYLNFKDPFTESD
                     CSYAIHLTDDFDSVIIGLQKKDRGY"
     gene            complement(6457..7293)
                     /locus_tag="VC122_001296"
     CDS             complement(6457..7293)
                     /locus_tag="VC122_001296"
                     /EC_number="2.7.1.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002993124.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD kinase"
                     /protein_id="HEP5641219.1"
                     /translation="MTQMNYTGKVKRVAIIANGKYQSKRVASKLFSVFKDDPDFYLSK
                     KNPDIVISIGGDGMLLSAFHMYEKELDKVRFVGIHTGHLGFYTDYRDFEVDKLIDNLR
                     KDKGEQISYPILKVAITLDDGRVVKARALNEATVKRIEKTMVADVIINHVKFESFRGD
                     GISVSTPTGSTAYNKSLGGAVLHPTIEALQLTEISSLNNRVFRTLGSSIIIPKKDKIE
                     LVPKRLGIYTISIDNKTYQLKNVTKVEYFIDDEKIHFVSSPSHTSFWKRVKDAFIGEI
                     DS"
     gene            complement(7268..7939)
                     /locus_tag="VC122_001297"
     CDS             complement(7268..7939)
                     /locus_tag="VC122_001297"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017771989.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GTP pyrophosphokinase family protein"
                     /protein_id="HEP5641220.1"
                     /translation="MTLDWEEFLDPYIQTVGELKIKLRGIRKQYRKQNRYSPIEFVTG
                     RVKSIESIKEKMILRGVIEENIAQDIQDIAGLRIMVQFVDDVEEVLALLRQRQDMTIV
                     YERDYIRNMKSSGYRSYHVVVEYPVDTIEGQKKVLAEIQIRTLAMNFWATIEHSLNYK
                     YGGDFPEEIKKRLEVTAKIALELDEEMRKIREDIREAQLLFDPVTRNLSDGVGNSDDT
                     DELYR"
     gene            8027..8605
                     /locus_tag="VC122_001298"
     CDS             8027..8605
                     /locus_tag="VC122_001298"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002984698.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CYTH domain-containing protein"
                     /protein_id="HEP5641221.1"
                     /translation="MTNLEIEYKTLLTKNEYNRLLSQMKHVTPVTQTNYYIDTKAFDL
                     KANKMSLRIRTFANSAELTLKVPEKVGNREYNVPLFLEQAKDMIKHGNLPESTALDII
                     ISKGIKPSALVTFGNLTTVRRETVIPIGKLALDYNLYANTKDYELELEVSDALQGKID
                     FDSFLSEHHIAFKYAKSKVARCINTLKKFNDK"
     gene            8745..9725
                     /locus_tag="VC122_001299"
     CDS             8745..9725
                     /locus_tag="VC122_001299"
                     /EC_number="2.7.6.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010922293.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribose-phosphate diphosphokinase"
                     /protein_id="HEP5641222.1"
                     /translation="MTERYADKQIKLFSLTSNLPIAEKIAKAAGIPLGKMSSRQFSDG
                     EIMINIEETVRGDDIYIIQSTSFPVNDNLWELLIMIDACKRASANTVNIVLPYFGYSR
                     QDRVAKPREPITAKLVANMLTKAGIDRVVTLDLHAVQVQGFFDIPVDNLFTVPLFAER
                     YSKLGLSGSDVVVVSPKNSGIKRARSLAEYLDSPIAIIDYAQDDSEREQGYIIGDVSG
                     KKAILIDDILNTGKTFAEAAKILERSGATDTYAVASHGLFAGGAAEVLETAPIKEIIV
                     TDSVKTKNRVPENVTYLSASDLIAEAIIRIHERRPLSPLFSYQPKGKNNA"
     gene            9722..10849
                     /locus_tag="VC122_001300"
     CDS             9722..10849
                     /locus_tag="VC122_001300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011888888.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine desulfurase"
                     /protein_id="HEP5641223.1"
                     /translation="MTYFDNAATTPLSPNVIRAMTAAMQDNFGNPSSIHFYGRRANKI
                     LRECRQAIARNLGASEQQIIVTSGGTESNNMAIKGYALAHQAKGKHLITTTIEHHSVL
                     HTMAYLEERFGFEVTYLPCQNGQINLSDLKQALRDDTILVSIMYANNETGDLLPIKDI
                     GNLLKDHQAAFHVDAVQAVGKLKIIPSELGIDFLSASAHKFHGPKGCGFLYSNGQPID
                     PLLHGGDQEGKRRASTENMLGIIGMAQALTDAMTCLDQSTDHIISLRHHLISLLEGLP
                     YYINQGTHYLPHVLNIGFLGYQNTILLTQLDLAGIAVSTGSACTAGAVNPSHVLAAYY
                     GDDSSRLKESIRISFSDQNSIEDVNQLAQTLKNILGGTDGF"
     gene            10839..>11058
                     /locus_tag="VC122_001301"
     CDS             10839..>11058
                     /locus_tag="VC122_001301"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002989831.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine desulfurase"
                     /protein_id="HEP5641224.1"
                     /translation="MAFEKEIKLKDCKYLYTISPNIKKYTLRDTTFSQTKVGHYQLIR
                     LLEKIPNSGDGFPLKITINKELTGFKLAI"
BASE COUNT         3458 a         2283 c         1870 g         3447 t
ORIGIN      
        1 atattttgtc aaatttaaat actattttta atttaaatgt tcggtttttg tatttatgaa
       61 ttgttacttc ttgaacttaa gagacgaaaa aatgccagtc ctatggacca acatttctgt
      121 ttatttctgg attaagcctg ttttcaacag ataatctcga gcgaccttat tagcatctgc
      181 tcctttaaca gatacctcgt agttcatgtc ctgcatctct ttttcagtaa tttttcccgc
      241 taattgatta agaatgcctt ttaattctgg atgttttgtt aataaggaag ttttcatcaa
      301 aggagctccc tgataaggtg gaaacaactg tttatcatct tttaaaactt tcaaatggta
      361 tttcgtgatt tcagcatccg tagagtaagc atctgttact tggatatctc ctgattgaat
      421 tgcttgataa cgaagagctg gctccatcgt cgccacagat agctgtaatc catattgaga
      481 ttgcattccc ttatagccat ctgctctgtc cttaaattcc agagtaaaac cagcctttaa
      541 agtatcagca tgcgctttta aatcagaaat ggtttcaatc tgatattcct tagccaattt
      601 ttctggcata gcaacagcat acgtattttg gtaagcaaat ggcttaagaa gggtcagttt
      661 atcttgctta gcaatgcctt ttttagcatc ctcatagacc tgcttagggt cattagacaa
      721 aggtggtttg tcgcgtaaaa gacttgatgt tatggttcct gtaaattcag gatagatatc
      781 aatatctcca gatttaaggg cttcatataa gaagctagtt ttcccaaaat tagatttcaa
      841 ctcaacactt atatctgatt gatcttcaat taattcctta tagagattaa tcagaatatc
      901 gggctctgct cccaatttac cggcaataac cactgtatct tttccttttg aaaactgact
      961 aagcgccata ggagtatacg atgctagcaa tgcaagtaag gtgattccaa aactaatcat
     1021 aatccgtcgc aaggaagctt tctcaaggta ctgtaacaaa ctattgaaaa taatagctag
     1081 cagagctgaa gaaatagccc ctatcagaat caggttagca ttattacgat caatccccaa
     1141 taagatgaaa gagccaagtc caccggctcc tatcaaggaa gctaaagtag ctgtgccgat
     1201 aatcatgact gctgacgtcc gcacacctga cataataaca ggcattgcaa ttggaatctc
     1261 aaacgttttt aaccgctccc atttggtcat tccaaaagct attcctgctt ccacgagact
     1321 tgggtcaata ccatttaatc ctgtgatggt gttttgtaaa atcggaaaaa tcgcatagat
     1381 aactaaagct gtcactgcag gcaacgttcc aattcccatt aaagggatga aaagaccaag
     1441 caaagccagt gagggaatgg tttgaaaaac acctgttacc tgtaacataa tgtctgacca
     1501 acgtttgctg cgactgagaa gggctgctaa aggcacacct attagcaagg cgatcataag
     1561 agataaaagg gaaatttgca ggtgttcccc cagagccgcc agccattcat taaaacggtt
     1621 ttggaaagtt acaaacaaac taggcatgct catcacctcc aaataaattg gccacaaaag
     1681 cattagcagg gtgggtctta atcatttctg ggcgatccag ttgcacaatt tctccctcat
     1741 ttaaaatagc tacgcggtct cctaatttaa tggcctcatc aatatcatgt gtcacaaata
     1801 caatcgtcat gtcaaattcc ttgtgaagac tcaacattaa ctcttgcaat tgtttacgtg
     1861 aaataggatc taaggctgaa aatggttcat ccatcaatag aattttagga tgagaaatga
     1921 tagcacgtac aataccaata cgttgctgtt cacctcccga taaatcactt gggtaacgat
     1981 caagataatc tttagcaggt aggcccacct tatccaataa ttcttcagtt ttttgtctaa
     2041 tttcttcagc tgaccattgc ttcatctcag ggataatcgc aatattttca gctactgtga
     2101 gattggggaa aagagcaatt tgctgcaaca cgtatccaat agatagcctc atctcacgta
     2161 aatctaactc tttctgtggg acgttattca gtaaaatatc tccagagctt ggttcaataa
     2221 ggcagttaat catttttaaa agtgtggttt tccccgatcc gctgggacca accagcacaa
     2281 aaaattcacg gtcattaatt tgaaaggtct gctcttgcaa aacctttgtc tgaccaaaag
     2341 ttttagaaac attattaaaa cgtatcatta tttctccaat tcattggtta aagtaacagc
     2401 ataaaagctg atgagttcat tcaacctgtc tctctgtcat tttttctcaa aacgttctga
     2461 gtaactacaa ggaatcactg catgccctac catttttaga caaacgcatc tactaattaa
     2521 agggactctt aggtatcctc agtaaggtag aaactaccaa ttctcaaaaa ctttttctct
     2581 cattattttc ttgtcgtttg ttgttgacca ttataaaaag tttcccacta taagtcaaat
     2641 aatgagacta tttctcctta ccccttatag aaaaacaggt cactttgtca gcctatctct
     2701 ccaaaattac cttatcaaaa atcctagact tgcctatgat agataagcag attagttgtc
     2761 ttttctagaa aatacggcta tcatacaatt atgaaaaatg ccatttccat tttagcgctc
     2821 ttaacatctg aaaaccaact tgaagatatg tttattagag agctttccca ccacttctca
     2881 cacctttttg aggtgactat tctcactagc aaagccaaca ttgagtcaaa tcaattgtcg
     2941 actttccaag gaattttcag tttccatgag catgacattg acttgcctac actctatttc
     3001 aaaacctctc aacatggtca gggattttta gtgacagaaa gtgtctttga tcaagcaaca
     3061 gcagtcttat cgttaagcca atacctcact agattttacc aaaaatttga tagacatttt
     3121 ttacagtacc ttcctttgca agcaaggtta tctgatgcaa atggaaacat catagtcgat
     3181 aatcatgctt ttaatggaag ttttttgcca actactgata aagaaataga agattggatt
     3241 cttgctgaat tgcgtttatc agataaccct tgtaaaacct tcctacttcc ttcgggttca
     3301 ttggatcata tttacatgca gcattatcaa gctcttaaaa atcctcaagg tcagctggtg
     3361 ggagttttgg ataccgttca agacattaaa ccgttattaa accagtattt agaagaaact
     3421 ggacaagcca ttgttggctg gtccgatgtc acatcaggcc cttccatttt agatcactaa
     3481 acttgatttt aaatagggag ttcagcctaa atcatcaacg cacaagaagc aatcatgaac
     3541 gcttgaagac ctcaaaacct tcttcttgat tgcttttttt gatgactaaa tcggtaagtc
     3601 tcacttataa agaataatcc ataccagtaa accatttttc taaagattat tattcttatg
     3661 gactcatccc ttgctagtca cgactaactg gttgcggtcc ataactttgc gacactggca
     3721 tcagctcaat acgattaaca ttaacatgag ggggctgatg aatcacccat gatacagtcg
     3781 acgcaatatc ttgaggttga atggcatgtg ctccttgata aatggccttc acccgtgcct
     3841 catcaccatt aaagcgaacc tttgaaaatt ctgtcccgtc acatagaccg ggttcaatat
     3901 tggtcacacg tatatgggta cctgctaaat cagctcgtaa attgagtgag aactgcttaa
     3961 caaaagcctt gctagcccca taaatattag ctcccggata gggaattgtc cctgcggttg
     4021 accctaaatt aataataata ccatcatctt tggagaccat atgtggtaat aattgacgtg
     4081 tgagataaat caacccaaca atattagtgt taatcatcgt catccaattc tcaaaatcgg
     4141 cttcataagc cttatcaagt cccaatgcca agcctgcatt attcactaaa atagtaatat
     4201 cttgccactg atttggtaga cttgtcaaag catcatcaat cgctttggta tctgtgacat
     4261 ccatctgcaa agggtaaaag ttgtccgacc caaagctcgt ttctaaggcc ttgagtttct
     4321 caagacgacg agaagcacca ataaccttta tcccatctgc aattaattgt tcgacaatgg
     4381 ctctgccaaa accggctgaa gcccctgtta ctaaggcaat atttcccata catttcctca
     4441 ccttcaaaaa aaacgagatg gtctggccaa ctcgttttta ataatgtcaa ttagtcttag
     4501 gataacttct aacacatcac ttagcggtta gaatcctata tcaaggggtc ttaagttacc
     4561 cttggctttc gatagcttgt gctgctgtaa taatagctaa tttgtaaata tcttcagcgc
     4621 ttgagccacg agataaatca ttaacaggtt tgttcaatcc ttgtaagatt ggtccaatag
     4681 catcaaacat tcctaaacgt tgtgcaattt tgtaaccaat atttccagat tgtaagtcag
     4741 ggaaaataaa agtattggct tggccagcaa ccgctgaatc tggtgctttg atagctgccg
     4801 tttcaggaac aaaggctgca tcaaattgca actcaccatc aagtgccaaa tcagggttca
     4861 accctttagc aatttctgtt gcttctctta ccttatcaac ttgaggcgct ttaccagaac
     4921 cttttgttga gaagcttaac atagcaattt taggatcaat atcaaagatt ttagctgttt
     4981 cagctgtgtt aacagcgatt tctgccaatt cttgtgctgt tgggtcaata ttgatagcac
     5041 aatcagcaaa cacgtaacgc tcactcgtat tttcgcgatt catcaagaaa acacctgaag
     5101 ttcttgaaat acctggtttg gttttaatga tttgaagggc tggacgaaca gtatcagctg
     5161 ttgaatgaat agcaccagat accattccat ctgctaatcc cattttaacc aacataacac
     5221 caaagtaatt cacatcacga agcattttat cagcatcttc aagcgttgct tttcctttac
     5281 ggatgtcaac aaaagcttct ttcattttgt caaaatccgc atattcattt ggattaatga
     5341 tagtatagtc ttgatcagcg aagcctaatt ttgtcaaaag gttgcgtacc tcttctgact
     5401 gtccaaggat aatcggttct aagagtcctt caaatttcaa acgagcagct gcacggacga
     5461 cgcgctcatc attaccttct gggaaaacaa tcttcatgtt tttaccaaga attttttctc
     5521 ttaaaccacc gaataaacta cgaatactca ttctttaata acctctatct tttttctgta
     5581 aacctatgat aacgctatca aagtcatctg tcaagtgtat tgcatagcta caatcacttt
     5641 ctgtaaatgg atctttgaaa ttcaaataat gacaatgtaa agcttgcctt gttattccta
     5701 agtccaaacg acctccgtaa aggtcatccc ctaaaagtgg aaaaccaata tgggcaaaat
     5761 gaactctgat ttgatgggta cgtcctgtat gcaatttgat atccactaaa tgcacatttt
     5821 ctgaatagcg cgccacaacc ttatagctag tcttggcata tttgcccata gggtcaactg
     5881 ccctggtaat aatactatct tttgaacgcc caattggagc aataatatct ccttcatctg
     5941 gcaacgtccc atttccagaa accagagcaa aataacgttt ctctattgag cgagtttgga
     6001 gttgcttgtc caaccttgca tgagcataac catgcttagc aaataacatc aagccactcg
     6061 tatctctgtc taaacgagtc acgatatgca cttgttggtc taggtaatga ttgtccacat
     6121 aatatgcctt gataaagtta gcaatggtat tggagtggat ggcacttgga atactggcaa
     6181 agccaatggg tttattaatc accaaaaaat gatcatcctc gtgaacaata tccaaatcaa
     6241 aagggatggc ttcaagcttt tcaaagggct cttcgtttgg aatgtcaatg gtgacaacat
     6301 ctcccacttg caataaataa atggcatttt gttcaatacc attcactaat atattaccgc
     6361 ccttatattt gattttagcc aatagtcctt tagagacatc atgacttttt aaaagggttt
     6421 ttacttttat tcgtttgtct gctacaaatt caaatctcat gagtcaatct ctccaataaa
     6481 ggcatccttg acccttttcc aaaagctcgt atgactcgga gaggaaacaa aatgaatttt
     6541 ctcatcgtcg ataaaatact ccaccttcgt cacatttttt aactgatagg ttttattatc
     6601 aatggaaatg gtataaattc ctaatcgttt tggcactaac tcaatcttat cttttttggg
     6661 aataatgatt gatgagccca aggttctaaa gacacggtta ttaagactgg aaatttccgt
     6721 caattgcagc gcttcaatcg tcggatgcaa gacagcacca cctaaagatt tattgtaggc
     6781 tgtgctccct gtcggggtcg atactgaaat cccatcacct cggaagcttt caaatttgac
     6841 atggttaata ataacatctg ctaccatcgt tttttcaata cgcttaaccg tcgcttcatt
     6901 caaagcacgc gctttaacca cacgaccatc atctaaagta atagcaactt ttaaaatagg
     6961 ataagagatt tgttctccct tgtcttttct taaattatca atcaatttat caacttcaaa
     7021 atccctataa tcggtataaa agccaagatg accggtgtgg attcctacaa aacgtacctt
     7081 atctaattct ttttcataca tgtgaaaggc agataagagc atcccatctc cgccaataga
     7141 aatcacaata tccggatttt tctttgaaag atagaaatca ggatcatctt taaatacgga
     7201 gaaaagtttg gaggcgacgc gtttactttg gtacttacca tttgcaataa tagcaactcg
     7261 ctttacctta cctgtataat tcatctgtgt catcactatt tcctacacca tcacttaaat
     7321 ttcgtgtcac ggggtcaaac aataattgag cctcccgaat atcttctcga atcttacgca
     7381 tttcttcgtc taattctaaa gcaattttag cagtcacttc aagacgcttt ttaatttctt
     7441 ctgggaagtc tccaccatat ttataattta aagaatgctc aatggtcgcc caaaagttca
     7501 ttgccaaagt acgaatctga atctccgcta agaccttttt ttgaccttca atagtatcaa
     7561 cagggtactc aaccacaaca tgataagacc tgtagccact ggacttcata ttgcgaatat
     7621 aatctcgttc ataaacaatt gtcatgtctt gtcgttgacg caatagagct aagacttctt
     7681 ccacatcatc gacaaactga accataatac gtagtccagc aatatcttga atatcttgtg
     7741 ctatattttc ttcaataaca ccacgtaaaa tcattttttc tttaatactt tcaattgact
     7801 taacacgtcc cgtaacaaat tcaatcggag aataacgatt ttgcttacga tattgcttgc
     7861 gaataccccg tagttttatt tttaattcac caactgtttg gatataagga tctaaaaatt
     7921 cttcccaatc taaggtcatc tattgtctcc tttcttgtca aagccaggat aataatataa
     7981 aattgaaata acaatttatt atagcacaaa ttaaaaggaa tttcccatga ctaacctaga
     8041 aatcgaatat aaaactctac ttacaaaaaa tgagtataac cgtcttttat ctcaaatgaa
     8101 acatgtgacg cctgttacac aaactaatta ctatattgat acaaaagcat ttgatttaaa
     8161 agctaacaaa atgtcattac gtattcgaac atttgcaaat agcgccgaat taaccttaaa
     8221 agtgccagaa aaagtgggga atcgtgagta caatgtccct ctttttcttg aacaagcaaa
     8281 agatatgatt aaacatggga atcttcctga atcaactgct cttgatatta ttatttcaaa
     8341 aggtatcaaa ccatctgcac tggtaacttt tggcaattta actaccgttc gtcgcgagac
     8401 agtgataccg ataggtaaat tggccttaga ctataaccta tatgccaata ccaaagacta
     8461 tgaattagaa ttagaagtaa gtgacgctct tcaaggaaag attgattttg attctttttt
     8521 atctgaacat cacattgctt tcaaatatgc taaaagcaaa gtagctcggt gtattaacac
     8581 cttaaaaaag ttcaacgata aataatggct aaaagcgaca gaaaaactga aatttttctg
     8641 tttttctgtt aaaataggga tgtcaattaa acacttttgg gtaatgtgac tcattaataa
     8701 aacagtttac ccaattttat tgaagttaag gagctaaatc tatcatgact gaacgatatg
     8761 ctgacaagca aatcaaactg ttctcactca catcaaatct tccaattgct gaaaaaattg
     8821 ctaaagctgc tggaatccct cttggaaaaa tgtcttcacg tcaattttcc gatggagaaa
     8881 ttatgatcaa tattgaagaa acagtgcgtg gagatgatat ctatattatt caatccacta
     8941 gttttcctgt caatgataat ctttgggaat tactcatcat gattgatgct tgtaaacgtg
     9001 caagtgctaa taccgtcaat attgtattgc catactttgg ttactctcgt caagaccgtg
     9061 tcgctaagcc ccgcgaaccg attactgcta aactagtagc taatatgctg actaaagctg
     9121 gtattgatcg tgtagtgacg cttgacttac atgctgttca ggtacaaggt ttttttgata
     9181 ttccagtgga taacctcttt acagtccctc tttttgcaga acgttacagt aaattaggct
     9241 tatcaggttc tgatgttgtt gtcgttagcc ctaaaaattc tggaattaaa cgtgctagaa
     9301 gcttggctga atatcttgat tccccgattg ctattatcga ctatgcacaa gatgattctg
     9361 aacgtgaaca aggttatatt attggtgatg tttctggtaa aaaagccatt ttaattgatg
     9421 atattttaaa tactgggaaa acctttgcag aagctgctaa aattcttgaa cgctcaggcg
     9481 ctactgatac ttacgcggtc gctagccatg gcttatttgc tggtggagct gctgaggtat
     9541 tagaaacagc tccaatcaaa gaaattatcg ttacagattc tgttaaaact aaaaatcgtg
     9601 tgccagaaaa cgttacttat cttagcgcca gtgatttaat cgcagaagct attattcgta
     9661 tccatgaaag aagaccctta agtccacttt tttcttatca acctaaaggc aaaaataacg
     9721 catgacttac tttgataacg ccgccaccac accacttagt cctaatgtga ttagggcgat
     9781 gacagcagct atgcaagata actttggtaa cccctctagt attcatttct acgggcgccg
     9841 agccaataaa atccttcgtg aatgccgaca agctatcgct agaaacttgg gggcaagtga
     9901 acagcaaatt atagtaacgt ccggtggaac tgaaagcaat aatatggcca taaaaggcta
     9961 tgctttagct catcaagcaa agggcaaaca tctcatcaca actactattg agcaccactc
    10021 tgtgcttcat accatggcct atctcgaaga gcgatttggt tttgaggtca cttatttgcc
    10081 ttgtcaaaat ggacaaataa atttatctga cttaaaacaa gccttgagag atgacaccat
    10141 tttggtaagc ataatgtatg ccaataacga aacaggtgac ctgttaccta ttaaggacat
    10201 tgggaatctg ctcaaagacc atcaagctgc ttttcatgtt gatgccgttc aagctgttgg
    10261 caaactcaag attattccta gcgaacttgg gattgacttt ttatctgctt ctgctcacaa
    10321 atttcatggt cctaaaggat gcggcttttt atacagtaac ggacaaccaa tcgaccctct
    10381 tcttcacggg ggtgatcaag aaggaaagcg acgcgcaagt actgaaaata tgttaggtat
    10441 tattggtatg gcacaagctt tgactgatgc tatgacttgt cttgaccaat caactgatca
    10501 cattatcagt ttacgtcatc acttgattag cctcctagaa ggactaccct actatattaa
    10561 tcaaggtact cattatcttc ctcatgttct caatattgga tttttaggct accaaaatac
    10621 tattttacta acacaacttg acttagcagg cattgcagta tcaactggtt ctgcttgtac
    10681 tgctggagct gttaatccaa gtcatgtttt agctgcgtac tatggggatg actcttctcg
    10741 tttaaaagaa tcaattcgta tcagtttttc agatcaaaat agcattgaag atgttaatca
    10801 attagctcaa acattaaaaa atattttagg aggaacagat ggcttttgaa aaagaaatta
    10861 agcttaaaga ctgtaaatac ctttatacaa tcagtccaaa cattaaaaaa tataccctaa
    10921 gagatactac atttagtcaa actaaagtag ggcactacca attaatacgt ttactcgaaa
    10981 aaatacctaa ttcaggggat ggtttccctt taaaaatcac tattaataaa gaactcacag
    11041 gttttaaatt agctatca
//
LOCUS       DATXIG010000045        10915 bp    DNA     linear   BCT 28-DEC-2023
DEFINITION  TPA_asm: Streptococcus pyogenes strain MGAS11113
            SAMN08555799-rid19682583.denovo.045, whole genome shotgun sequence.
ACCESSION   DATXIG010000045 DATXIG010000000
VERSION     DATXIG010000045.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN08555799
            Sequence Read Archive: SRR6740495
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pyogenes
  ORGANISM  Streptococcus pyogenes
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 10915)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10915)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-OCT-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-OCT-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001918058.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001918058.2
            Genome Coverage       :: 368x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/12/2023 11:23:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 1,806
            CDSs (total)                      :: 1,769
            Genes (coding)                    :: 1,722
            CDSs (with protein)               :: 1,722
            Genes (RNA)                       :: 37
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1, 1 (5S, 23S)
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 47
            CDSs (without protein)            :: 47
            Pseudo Genes (ambiguous residues) :: 0 of 47
            Pseudo Genes (frameshifted)       :: 30 of 47
            Pseudo Genes (incomplete)         :: 20 of 47
            Pseudo Genes (internal stop)      :: 9 of 47
            Pseudo Genes (multiple problems)  :: 11 of 47
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10915
                     /organism="Streptococcus pyogenes"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN08555799-rid19682583.denovo.045"
                     /strain="MGAS11113"
                     /serotype="Emm28"
                     /isolation_source="normally sterile site"
                     /host="Homo sapiens"
                     /db_xref="taxon:1314"
                     /country="Finland"
                     /collection_date="2002"
                     /collected_by="National Public Health Laboratory"
     gene            complement(<1..671)
                     /gene="spej"
                     /locus_tag="VC122_001302"
     CDS             complement(<1..671)
                     /gene="spej"
                     /locus_tag="VC122_001302"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010921949.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="streptococcal pyrogenic exotoxin SpeJ"
                     /protein_id="HEP5641225.1"
                     /translation="MKRLIKTIILVIIIFHGYGSVKSDSEKIKDVKLQLNYAYEIRPV
                     DYTNCNIDYLTTHDFYIDVSSYKKKNFSVDSEVESYITTKFTKNQKVNIFGLPYIFTR
                     YDVYYIYGGVTPSVNSNSENSKIVGNLLIDGVQQKTLINPIKIDKRIFTIQEFDFKIR
                     QYLMQTYKIYDPNSPYIKGQLEIAINGNKHESFNLYDATSSSTRSDIFKKYKDNKTIN
                     MKDFS"
     gene            complement(873..1010)
                     /locus_tag="VC122_001303"
     CDS             complement(873..1010)
                     /locus_tag="VC122_001303"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEP5641226.1"
                     /translation="MLTYHELKKAVANRYIKGDMISITRKLVSSLILSFLIELYEYQE
                     F"
     gene            1242..1525
                     /locus_tag="VC122_001304"
                     /pseudo
     CDS             1242..1525
                     /locus_tag="VC122_001304"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014407356.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            1699..2202
                     /locus_tag="VC122_001305"
     CDS             1699..2202
                     /locus_tag="VC122_001305"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEP5641227.1"
                     /translation="MKTRPKRFLNLATLSLALLGTTLLMAQPVKADNDTRESQERVDQ
                     RIEDPYDDGKNAGRKDGYESGNHKGISEEPPTKTPEPPTNPYSRDRVKSKKYRDGYQE
                     WYVYGYYEGWHRANDEDKNKESSDHSRDQDLEGTEQTDTEYSAIISTLYGIAGVVLEY
                     VLSWFSI"
     gene            2862..3098
                     /locus_tag="VC122_001306"
     CDS             2862..3098
                     /locus_tag="VC122_001306"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018364244.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2829 domain-containing protein"
                     /protein_id="HEP5641228.1"
                     /translation="MTFEEILPGLKAKKKYVRTGWGGAENYVQLFDTLEVDGKVLQAT
                     PYFLIHVTGEGEGFSMWAPTPCDVLAEDWIEVND"
     gene            3091..3789
                     /locus_tag="VC122_001307"
     CDS             3091..3789
                     /locus_tag="VC122_001307"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002990841.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-oxoacyl-ACP reductase"
                     /protein_id="HEP5641229.1"
                     /translation="MTKVVLVTGCASGIGYAQARYFLRQGYHVYGVDKSDKPDLSGNF
                     HFIKLDLSSELSPLFKVVPSVDILCNTAGILDAYKPLLDVSDEEVEHLFDINFFATVK
                     LTRHYLRRMVEKQSGVIINMCSIASFIAGGGGVAYTSSKHALAGFTRQLALDYAKDQI
                     HIFGIAPGAVKTAMTASDFEPGGLAEWVARETPIGRWTEPDEVAELTGFLASGKARSM
                     QGEIVKIDGGWTLK"
     gene            3865..4824
                     /locus_tag="VC122_001308"
     CDS             3865..4824
                     /locus_tag="VC122_001308"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002990834.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HEP5641230.1"
                     /translation="MKLAVLGTGMIVKEVLPVLQKIDGIDLVAILSTVRSLKTAKDLA
                     KAYHMPLATSEYEAVLGNEEIDTVYIGLPNHLHFAYAKEALLAGKHVICEKPFTMTAG
                     ELDELVVIAKKRKLILLEAITNQYLNNMTFIKEHLDQLGDIKIVECNYSQYSSRYDVF
                     KRGDIAPAFNPKMGGGALRDLNIYNIHFVVGLFGRPKTVQYLANVEKGIDTSGMLVMD
                     YEQFKVVCIGAKDCTAEIKTTIQGNKGSLAVLGATNTLPQVQLSLHGHEAQVVNHNKY
                     DHRMYEEFVAFRDMIDQRDFEKVNQALEHSRAVMAVLERAVHS"
     gene            5157..6494
                     /locus_tag="VC122_001309"
     CDS             5157..6494
                     /locus_tag="VC122_001309"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014635322.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HEP5641231.1"
                     /translation="MEEDYNKREPEKFTQFLRRQKVVFFVAFFGYVCAYLVRNNFKLM
                     SNTIMVQNGWDKAQIATLLSCLTVSYGLAKFYMGALGDRVSLRKLFSISLGASALICI
                     LIGFFNSSMVVLGILLVLCGVVQGALAPASQAMIANYFPNKTRGGAIAGWNISQNMGS
                     ALLPLTIALLTSMGLVVPANGNILLAFLIPGVLVFLFALCCWKLGGDNPESEGLDSLR
                     TMYGDAGESAVASEEEKHNLSYWQLIWKYVFCNPSLLLVAAVNVALYFVRFGIEDWMP
                     IYLSQVANMSEAHIHFAISMLEWVAIPGSLVFAWLAVRYPNKMAKIGAIGLFVLAAIV
                     FVYERLTATGAPNYFLLLVIAGILGSLIYGPQLIVNILTINFVPLNVAGTAIGFVGVT
                     AYLIGNMGANWLMPILADGFGWFWSYIVVAALSAFSAVGYLILAKREEEIIKD"
     gene            6667..8049
                     /gene="glmU"
                     /locus_tag="VC122_001310"
     CDS             6667..8049
                     /gene="glmU"
                     /locus_tag="VC122_001310"
                     /EC_number="2.3.1.157"
                     /EC_number="2.7.7.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015016740.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional UDP-N-acetylglucosamine
                     diphosphorylase/glucosamine-1-phosphate
                     N-acetyltransferase GlmU"
                     /protein_id="HEP5641232.1"
                     /translation="MTNYAIILAAGKGTRMTSDLPKVLHKVSGLTMLEHVFRSVKAIS
                     PEKSVTVIGHKSEMVRAVLADQSAFVHQTEQLGTGHAVMMAETQLEGLEGHTLVIAGD
                     TPLITGESLKSLIDFHVNHKNVATILTATAQDPFGYGRIVRNKDGEVIKIVEQKDANE
                     YEQQLKEINTGTYVFDNKRLFEALKCITTNNAQGEYYLTDVVTIFRANKEKVGAYILR
                     DFNESLGVNDRVALATAETVMRQRITQKHMVNGVTFQNPETVYIESDVEIAPDVLIEG
                     NVTLKGRTHIGSGTVLTNGTYIVDSEIGDNCVVTNSMIESSVLAAGVTVGPYAHLRPG
                     TTLDREVHIGNFVEVKGSHIGEKTKAGHLTYIGNAQVGSSVNVGAGTITVNYDGQNKY
                     ETVIGDHAFIGSNSTLIAPLEVGDHALTAAGSTISKTVPIDSIAIGRSRQVTKEGYAK
                     RLAHHPSRSK"
     gene            8080..8634
                     /locus_tag="VC122_001311"
     CDS             8080..8634
                     /locus_tag="VC122_001311"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014635324.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX hydrolase"
                     /protein_id="HEP5641233.1"
                     /translation="MKFEEKTLKRQTVFDGHIFKVVVDDVELPNNLGQSKRELIFHRG
                     AVAVLAITPERKILLVKQYRKAIERVSYEIPAGKLEIGEEGSKLKAAARELEEETAYT
                     GTLTFLYEFYTAIGFCNEKITLFLATDLTQVANPKPQDDDEVIEVLELTYQECMDLVA
                     QGKLADAKTLIALQYYALHFGGEQ"
     gene            8634..8885
                     /locus_tag="VC122_001312"
     CDS             8634..8885
                     /locus_tag="VC122_001312"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010921956.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEP5641234.1"
                     /translation="MGRPLLTDDIIEKARRMETFESDDAVDFDTKVMTLPEKDDKARI
                     YKSRRIENAKRSQLQSKLNVILIAVMLLIAILVYAIFYL"
     gene            8905..9600
                     /locus_tag="VC122_001313"
     CDS             8905..9600
                     /locus_tag="VC122_001313"
                     /EC_number="3.2.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002993071.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5'-methylthioadenosine/adenosylhomocysteine
                     nucleosidase"
                     /protein_id="HEP5641235.1"
                     /translation="MKIGIIAAMEEELSLLLANLLDAQEHQVLSKTYYTGRFGKHELI
                     LVQSGVGKVMSAMTVAILVEHFKAQAIINTGSAGAVASHLAIGDVVVADRLVYHDVDA
                     TAFGYAYGQMAGQPLYYDCDPQFVAIFKQVLKHEKTNGQVGLIATGDSFVAGQDKIDQ
                     IKTAFSDVLAVEMEGAAIAQAAHTAGKPFIVVRAMSDTAAHDANITFDQFIIEAGKRS
                     AQILMTFLENLPV"
     gene            9751..10092
                     /locus_tag="VC122_001314"
     CDS             9751..10092
                     /locus_tag="VC122_001314"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002993073.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEP5641236.1"
                     /translation="MTLKKHYYLLSLLALVTVGAAFNTSQSVSAQVYSNGRYRQGLTD
                     EKSKLQYSKDNAKLQLRNILNGYQSDLGRHYSSYYYYNLRTVMGLSKEQEIEKYYEEL
                     KNKLHDMYNHY"
     gene            complement(10191..10838)
                     /locus_tag="VC122_001315"
     CDS             complement(10191..10838)
                     /locus_tag="VC122_001315"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002985785.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal-dependent transcriptional regulator"
                     /protein_id="HEP5641237.1"
                     /translation="MTPNKEDYLKCIYEIGEQEPKITNKMVAEKMHVSAPAVSEMIKK
                     MISQGWIVKDKAKGYLLKDKGYALVANLYRKHRLIEVFLIHQLGYNTQEVHQEAEVLE
                     HTVSDTFIDRLDKILDFPDFCPHGGTIPRYGQPLVEMNTTTLNTITELGRFRLSRIHD
                     HFDLIQYLEAHHLSINTELTLTQIDTFAKTYTICYGDKELVIPENIAKQLYVTAL"
BASE COUNT         3299 a         1881 c         2379 g         3356 t
ORIGIN      
        1 tggctgaaat ctttcatatt tatagtctta ttgtctttat attttttaaa aatatcactc
       61 cttgtactag atgaggttgc atcgtataag ttaaaacttt catgtttatt gccattgatc
      121 gcaatttcta attgcccttt tatgtatgga gaattaggat cataaatttt gtatgtttgc
      181 ataagatatt gtctgatttt gaagtcaaat tcttgaatcg taaaaatacg tttatctatt
      241 tttatgggat ttattagtgt tttttgctgg actccatcta ttagtaaatt acctacaatt
      301 ttactatttt ccgaattact gtttactgat ggtgtaaccc caccatatat ataataaaca
      361 tcataacgag taaatatgta cggaagacca aaaatattta ctttttgatt tttcgtaaac
      421 tttgttgtaa tatagctctc gacctcagaa tcaactgaaa aatttttctt tttataactg
      481 gaaacatcaa tataaaaatc atgagtggtc aagtaatcaa tattacaatt cgtataatct
      541 actggtctga tttcgtatgc gtaatttaat tgtagcttaa cgtctttaat cttttcacta
      601 tctgatttta cacttccgta cccatgaaag attattataa ctaaaattat tgtttttatt
      661 aatcttttca tacctctcct cgctcacccc gtgccccaca aactacttta tcctaaaaaa
      721 caataaaatc aacattgagg acttttttgg ttcccataat tgttaactat gtcattttag
      781 agcaatcata tagagactaa gatataattt taccaaagtt ttgaaggcta ttgaagagtg
      841 actttctccc ttaatttatt taattaaaga tactaaaatt cttgatattc ataaagctca
      901 atcaggaagg acaaaatcaa agagcttacc agttttctgg taatgctgat catgtctcct
      961 ttgatatacc tgttagctac agcttttttc aattcgtgat atgttaacat ttttatcctc
     1021 ctacttaatc tattcgtaaa aaatctctta aaatagacat ttaaaaaaaa gtccattata
     1081 acggatatgc ccgtgtgcga atacatgttc gggtaaatat tttaaatcaa gatgtttgat
     1141 gtttgtgtgt gcaaaaacaa aaaaatcgcc ctcgattgat gatatgatac gtcttaagta
     1201 gacaggctaa ataaccaaaa atctactcaa gaggtatttt tatgccagaa aagaaagggc
     1261 tgctcgtctt aagcatggta gtgagccttg tgtccctgct tgatcatatc ttcttttttg
     1321 ctacttatag ttatctttac ttgccacttg tagcaagtag tctagctatt attagtctgg
     1381 tgcaaaacaa aggaaggcaa aaggtttggt cgatagctgc tttagccgta gcagtagtga
     1441 cctataccca acggttattc cttatcaaat gcaaagtccc tccctcttat ataaactatc
     1501 aaatactttc aaaaacagtt cctaaagctc aaaaaggtta tctaactgct ccaagcaggc
     1561 actttctgtg ataaggatag cttgccattt ttttctaatc atttgatgtt taaaatgata
     1621 aaaaaatgaa cttgaaaatg aaagaatgag aggtgtaaac taaaagtacc aaataaacaa
     1681 tgaatacgga gtgattttat gaaaacaaga ccaaagcgct ttttaaatct agcaaccctt
     1741 agcttggccc ttttgggcac aaccttacta atggcacaac cggtaaaggc ggataatgat
     1801 actagggagt ctcaagagcg cgtagaccaa agaatagaag atccttatga tgatggaaaa
     1861 aatgctggga gaaaggatgg ttatgaatca gggaatcata agggaatctc tgaagagcct
     1921 ccaactaaaa ctcctgaacc tccgactaat ccttatagcc gtgatagggt gaaaagcaag
     1981 aagtatagag atggttatca ggaatggtat gtctatggat actatgaagg ttggcataga
     2041 gcaaatgatg aggataaaaa taaagagtct tctgaccatt ctcgagatca ggatttagaa
     2101 ggtacagaac agacagatac tgaatattca gcaattatca gtacattgta tggaattgct
     2161 ggagttgtat tggaatatgt gctatcttgg tttagtatct aagatttagt tttggttact
     2221 ggataatggt ttaaatattt attattatct aaaaatggcg tgatcaaatt ttgatgattt
     2281 ggttaatatt gtttagaaca gactgaagac aaagctctta gtaactaaat tctaagggct
     2341 ttgtcttcag tctgaggcac cttacggggt gtcttttatt atttaggtgg cttaatgaat
     2401 tggttaaaat ctttttagtt tagtaaaatt gctatttaag gttagccacc tctcgtatgg
     2461 tcacagttga tgaattatgc tatagtatag acataaaaaa tagttcaaat cccttcttgc
     2521 ctgttggtgg attttgaact gaaagcctct ctgattgcag ttggtaggct tttttgatgc
     2581 tttttcgtac tttttttatt tattacgcta aaaaatgtta tacttgatga agaaaaagga
     2641 gagtttagaa cgttaagata cctcacagca gattgataga ggatatggaa aaaggttagc
     2701 tttcccaaga gatgccgttt gggaatggta taacaataac tggcagaagt tataagtaaa
     2761 acaagaacaa attaactcag atgattttta gacgttccca cgcgtgtgcg aattaattgg
     2821 aaaaaaatga gaaaatacga tatacaaaaa aggagaacaa tatgacattt gaagaaattt
     2881 taccaggttt aaaagccaag aaaaaatatg tacgtacagg ttggggtggc gctgaaaatt
     2941 atgtgcagtt gtttgatacc cttgaagtgg atgggaaagt actacaggcg acgccttatt
     3001 ttctgattca tgttactggt gaaggtgaag gtttttccat gtgggcaccg acaccatgtg
     3061 atgtgttagc agaggattgg atcgaggtca atgactaaag ttgttttagt gacgggctgt
     3121 gctagtggga ttggttatgc acaggcccgc tattttttga gacaagggta tcacgtatat
     3181 ggtgtggata agtcggataa gccggactta agcggtaact ttcactttat caaattggat
     3241 ttatcctctg agttatcccc actctttaaa gtggtcccca gtgttgatat tttgtgtaat
     3301 actgcaggga ttttagatgc ctacaagcca cttttagacg ttagtgatga agaagtggaa
     3361 cacctatttg atattaattt ttttgcgacg gtgaagttaa cacgtcatta tcttagacgt
     3421 atggtggaaa agcagtcagg tgttatcatc aatatgtgct caatagcaag ttttatagcg
     3481 ggtggtggtg gtgttgccta tacgagttct aagcatgcgc ttgcgggctt cacgcgtcaa
     3541 ttggctttgg attacgccaa agatcagatt cacatctttg gtatagcgcc aggtgctgtc
     3601 aaaaccgcta tgacggctag cgactttgag cctggtggct tagcagaatg ggtagctcgt
     3661 gagacgccaa ttgggcgttg gactgaacca gatgaagttg ctgaattaac gggatttctt
     3721 gcgtcaggaa aagcgagatc aatgcaaggt gaaattgtca aaattgacgg tggctggact
     3781 ttgaaatgat cacatgcctt agcttgagct ggcgcagtaa gccctagagg tgtgatgagt
     3841 caaacagtca aaggagatga acaaatgaaa ttagcggttt tggggacagg tatgattgtt
     3901 aaggaagttt tgcctgtctt gcagaaaatt gatggtattg atctagttgc tatcttatca
     3961 acagttagaa gtttaaagac agctaaagac ctcgctaagg catatcacat gcctctagct
     4021 acgagtgaat atgaggctgt cttaggcaat gaggagattg acacggtcta tattggactg
     4081 ccaaatcacc tgcattttgc ctatgctaag gaggctcttc tggctggtaa acatgtcatt
     4141 tgtgaaaagc cctttaccat gacagcagga gaattagatg aactggtggt aatcgctaaa
     4201 aaacgaaagc ttattttgtt agaagcgatt acgaatcagt atttaaacaa catgaccttt
     4261 atcaaagagc atcttgacca acttggcgat atcaaaattg tagaatgcaa ttattcacaa
     4321 tattcttcac gctacgatgt cttcaaacga ggtgatatcg cgccagcttt taatcctaaa
     4381 atgggtggtg gagctttacg tgatttaaac atttacaaca ttcattttgt ggtaggatta
     4441 tttggtcgcc ctaaaacggt tcaatattta gccaatgtcg aaaaaggcat tgacacatca
     4501 ggcatgcttg tcatggacta tgagcagttt aaagtggtgt gtatcggggc caaagactgc
     4561 actgcggaaa tcaaaacaac catccaaggt aataaagggt cactggcagt gcttggagca
     4621 acaaataccc ttcctcaggt tcagttaagt ctacacggtc acgaagcaca agtggttaat
     4681 cacaacaagt atgaccaccg gatgtatgaa gagtttgtcg cttttaggga catgattgac
     4741 caaagagact ttgagaaagt gaatcaggct ttggagcata gccgagctgt catggctgtg
     4801 ttggagcgag ctgttcattc gtgatggtaa tctggtagcc attgcctttt gtgcagttgc
     4861 tatggtaatg gttgaaagac gtgttttcac gagggttaac cccctcgtgc ttttttggta
     4921 aaaataactt gttcaggatt tgggccttct ttgtgagatg agagatgcct gacgaattga
     4981 tttgattaaa ataatgataa agccaaacga tttaagaaat gaccgttcgg aaattctgcc
     5041 ccaaaaggtc tctagaagca gtaaaatcga atttgtaagc gttgacactg ctgcataact
     5101 ctgttacact aagagaatgt catgacaaaa attacagtgt aggagtttaa ttgtttatgg
     5161 aagaggatta taacaaaaga gaacctgaga agtttacgca atttctgcgc cgtcaaaagg
     5221 tcgtcttttt tgtcgcattt tttggttatg tgtgtgctta cttggtacgc aacaatttca
     5281 aactaatgtc aaacactatt atggtccaaa acggttggga caaggctcaa attgcgacct
     5341 tgttatcgtg tttgaccgtt tcttatggct tagcaaaatt ctatatgggg gccttagggg
     5401 atcgtgttag cttgagaaag ctcttttcga tcagcttggg tgcaagcgct ttgatttgta
     5461 ttttaattgg ttttttcaat agttcaatgg tagtgttagg gatactgcta gtcttgtgtg
     5521 gtgttgtaca aggtgccttg gcacctgcct cacaagccat gattgccaat tattttccaa
     5581 ataaaacacg cggcggagcc atcgctggat ggaacatttc tcaaaacatg ggatcagccc
     5641 tcttgccatt aaccatcgcc ttgctcacca gtatgggctt ggtggtacca gctaatggca
     5701 atattttact tgccttttta atccctggtg ttttagtgtt cttgtttgct ttgtgttgct
     5761 ggaagcttgg cggtgataat cctgagtctg agggacttga ctctcttcga acgatgtatg
     5821 gtgatgcggg cgaatcagct gttgccagtg aagaagaaaa gcataacctg tcttattggc
     5881 aactcatctg gaaatacgtt ttttgtaacc cgtcgctctt acttgttgct gctgtcaatg
     5941 tggcccttta ttttgttcgt tttgggattg aagactggat gccgatctac ttgtcacaag
     6001 tagccaatat gtcagaggcc catattcatt ttgcgatttc aatgttagag tgggtagcta
     6061 ttccaggctc gctggtattt gcgtggttag cggttcgtta tcctaataaa atggccaaga
     6121 ttggggctat tgggcttttt gtgttagcgg ctattgtgtt tgtctatgaa cgcttgactg
     6181 ccacaggtgc tccaaactat ttcttgttgc ttgttattgc aggtatttta gggtcattga
     6241 tttatggccc acagttgatc gtgaatattt taacaatcaa ctttgttcct ttaaacgttg
     6301 caggaacagc gattggtttt gtaggagtaa cagcttatct catcggcaat atgggagcaa
     6361 actggctgat gccgattttg gcagatggtt ttggctggtt ttggtcatat attgtcgttg
     6421 cagccttatc tgctttttca gcggttggtt atttaatttt agccaaacgt gaggaagaaa
     6481 tcatcaaaga ttagctaggc aaatgcctaa gaaagacaga cttattgctg aagtcctgtc
     6541 ttttttagtc atcacaagcc gcaagtaaca ctataaaaga caagaaaatg ctagggtgtt
     6601 tcaggaaaaa aagagaaaat atgctataat gagaaggtat ttaaattata tagtaaggaa
     6661 aaacaaatga caaattatgc cattatttta gcagcgggca aagggactcg catgacttct
     6721 gatctcccaa aagttcttca taaagtatct ggactaacca tgcttgagca tgtttttcgt
     6781 agtgtaaaag cgattagtcc tgaaaaatcc gtgacggtta ttggtcacaa atcagaaatg
     6841 gtacgtgctg tcttagcaga tcaatcagcc tttgtccatc aaacagagca gttaggaact
     6901 ggccatgccg tgatgatggc agaaacacaa ctagaaggac tagaagggca taccttggtg
     6961 attgcaggag atactccctt gatcactgga gaaagcctga agagtctgat tgattttcat
     7021 gtgaaccata aaaatgtcgc cactatttta acagctacgg ctcaagatcc atttggttat
     7081 ggtcgtattg ttcgcaacaa agatggcgaa gtgatcaaga ttgttgagca aaaagacgcc
     7141 aatgagtatg agcaacaatt aaaagaaatc aatacaggaa cttatgtgtt tgataacaag
     7201 cgtctctttg aagcgctcaa atgcatcact accaacaatg ctcaaggaga gtattacctg
     7261 actgatgttg tgactatttt tagagcaaac aaagaaaagg taggagccta tatcctgaga
     7321 gacttcaatg aaagtcttgg tgttaatgat cgtgtggcct tagcaacagc tgaaacagtg
     7381 atgcgccagc gcattaccca aaaacatatg gtcaatgggg tgactttcca aaatcctgaa
     7441 accgtttata ttgaaagcga tgttgagatt gctccagatg tccttatcga aggaaatgtg
     7501 accttgaaag ggcgtacaca tatcggctca ggaactgtct tgacgaatgg tacttatatt
     7561 gtggattctg aaattggtga taactgtgtt gttactaatt caatgatcga atcatctgtt
     7621 ttagctgcag gagttacggt tggcccttac gcccaccttc gaccaggaac gaccttagat
     7681 agggaagtcc atatcggtaa ctttgttgag gtcaagggtt ctcacattgg tgaaaaaaca
     7741 aaggctgggc atctgactta tattggaaac gctcaggttg ggtcttcagt caatgttggg
     7801 gctggaacca tcactgtcaa ctacgacggc caaaacaaat atgaaacagt cattggagat
     7861 cacgctttta ttgggagcaa ctcgactctc attgcacctt tggaagttgg ggatcacgct
     7921 ttaacagcag caggttcaac gatttcaaaa acggttccta tcgatagcat tgctattggt
     7981 cgtagccgcc aagtcacaaa agaaggttat gccaagcgcc tagcgcatca cccaagtcga
     8041 agcaaataat tcaagatcta tttagtaagg aggccaggca tgaaatttga agaaaaaacg
     8101 cttaagagac aaacagtttt tgatggtcat atttttaaag ttgttgtgga tgatgttgaa
     8161 cttcccaaca atctggggca atccaagcga gaattaattt ttcatcgtgg tgccgttgct
     8221 gttttggcta ttactcctga acgtaagatt ctccttgtca aacaataccg taaggctatt
     8281 gaacgtgtct catacgagat cccagcgggt aagctagaga ttggtgagga aggctcaaag
     8341 ctaaaagctg ctgcacgtga gctagaagaa gaaacagcct atacggggac attgacgttt
     8401 ttgtatgaat tttatacagc gattggtttt tgcaacgaaa aaatcacact tttcttagcg
     8461 acagacctaa cacaagttgc caatcccaaa cctcaagacg acgatgaagt tatcgaggtc
     8521 ttagagttga catatcaaga gtgcatggac ttagtcgctc aaggtaaact tgctgacgct
     8581 aaaacgttga ttgctttgca atactatgcc ttacattttg gaggtgagca gtgatgggaa
     8641 ggccattgtt gaccgatgat atcatcgaaa aagcgagacg aatggaaaca tttgagtcag
     8701 atgatgctgt agactttgac acaaaagtaa tgacgctacc agaaaaagat gataaagcac
     8761 gcatttataa aagccgtcgg attgaaaatg ccaagcgcag ccaattgcaa tctaaattaa
     8821 atgtgatttt gatagctgtt atgctgttaa ttgccatctt ggtatatgct atcttttatc
     8881 tttaaaaaaa cgtaaggaaa tgatatgaaa ataggaatta ttgctgctat ggaggaggag
     8941 ttgtccctgc tcttagcaaa tcttttggat gctcaagaac atcaggtgct ttcaaaaact
     9001 tactatacgg gtcgttttgg caagcatgag ctcattttgg tacagtcagg tgttggaaaa
     9061 gtgatgtctg ctatgacagt tgcgatcttg gttgagcatt tcaaagccca agctattatc
     9121 aatacaggat cagcaggggc agtcgcttca catttagcga ttggagatgt ggtggttgca
     9181 gatcggttag tgtatcatga tgtggatgct actgcatttg gttatgctta tggtcaaatg
     9241 gctggtcaac cattatacta tgattgtgat ccacagtttg tggctatctt caaacaagtc
     9301 ctaaaacacg aaaagacaaa tggccaagtt ggtttgattg caacaggaga cagttttgtt
     9361 gctggtcagg acaagattga ccagatcaaa acagctttta gcgacgtgtt agcagtagaa
     9421 atggaaggcg cagctattgc tcaagcagca catactgcag ggaaaccctt catcgttgtt
     9481 cgtgcgatga gtgataccgc agcgcatgat gccaatatta cttttgatca atttattata
     9541 gaagctggca agcgttcggc ccagatatta atgacatttt tagaaaactt acctgtttaa
     9601 ctcaccttat cgtgagacaa ttagctggat aatctctatt ttaggagtct ggtcacttct
     9661 tgttttgaaa cctaaaaata agaattgcaa tcacttgacc aaacgctata ataggttaag
     9721 taacgataga ttactaaaag gagtctaaaa atgacactaa aaaaacacta ttatcttctc
     9781 agtctgctag ctcttgtaac ggttggtgct gcctttaaca caagccagag tgtcagtgca
     9841 caagtttata gcaatggaag atatcgtcaa ggtttgactg atgaaaaatc aaagctgcaa
     9901 tatagtaaag acaacgccaa acttcaattg agaaatatcc ttaacggcta ccaaagtgac
     9961 ctagggagac actactctag ctattattac tacaacctaa gaaccgttat gggactatca
    10021 aaagagcaag agattgaaaa atattatgaa gagcttaaga acaagttaca tgatatgtac
    10081 aatcattatt aagttaaagg cttaaaaatg gagactagcc cctcaaaaaa agaggccaca
    10141 ctcatttaaa tttaaatgag tgtggcctct ttttgaggag acgcaagggg ttaaagggct
    10201 gtgacataga gttgcttagc aatattttca gggatgacaa gttccttgtc gccataacaa
    10261 atcgtatagg tttttgcaaa ggtatcaatt tgagttaagg ttagctcggt attgatactc
    10321 aggtgatgtg cttctagata ctggattaaa tcaaagtggt cgtggattct gcttaggcga
    10381 aaccgtccca actccgtgat ggtgttgaga gttgtagtgt tcatttcgac aagaggctga
    10441 ccatagcgag gaatagtccc tccgtgtggg cagaaatctg gaaaatcaag gatcttgtct
    10501 aaacggtcga taaaggtgtc tgagacagtg tgctctaaga cttcagcttc ttggtgcact
    10561 tcttgggtat tatagcccag ctggtgaatc aaaaatacct caatcaagcg gtgtttgcgg
    10621 tagaggttag caacaagagc atagcctttg tcttttagca aatagccttt tgctttatct
    10681 ttgacaatcc agccttgtga aatcattttt ttaatcatct ctgaaacggc aggtgccgaa
    10741 acgtgcatct tttctgcaac cattttattt gtaatcttgg gttcttgttc tccaatttca
    10801 tagatacatt tcaagtaatc ttctttatta ggcgtcatat gtttggcctt tctcttattt
    10861 ataatgatta ttatatcaga atttttagga aaaagcttga caaaaagcct aaaag
//
LOCUS       DATXIG010000046        10718 bp    DNA     linear   BCT 28-DEC-2023
DEFINITION  TPA_asm: Streptococcus pyogenes strain MGAS11113
            SAMN08555799-rid19682583.denovo.046, whole genome shotgun sequence.
ACCESSION   DATXIG010000046 DATXIG010000000
VERSION     DATXIG010000046.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN08555799
            Sequence Read Archive: SRR6740495
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pyogenes
  ORGANISM  Streptococcus pyogenes
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 10718)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10718)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-OCT-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-OCT-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001918058.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001918058.2
            Genome Coverage       :: 368x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/12/2023 11:23:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 1,806
            CDSs (total)                      :: 1,769
            Genes (coding)                    :: 1,722
            CDSs (with protein)               :: 1,722
            Genes (RNA)                       :: 37
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1, 1 (5S, 23S)
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 47
            CDSs (without protein)            :: 47
            Pseudo Genes (ambiguous residues) :: 0 of 47
            Pseudo Genes (frameshifted)       :: 30 of 47
            Pseudo Genes (incomplete)         :: 20 of 47
            Pseudo Genes (internal stop)      :: 9 of 47
            Pseudo Genes (multiple problems)  :: 11 of 47
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10718
                     /organism="Streptococcus pyogenes"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN08555799-rid19682583.denovo.046"
                     /strain="MGAS11113"
                     /serotype="Emm28"
                     /isolation_source="normally sterile site"
                     /host="Homo sapiens"
                     /db_xref="taxon:1314"
                     /country="Finland"
                     /collection_date="2002"
                     /collected_by="National Public Health Laboratory"
     gene            78..599
                     /locus_tag="VC122_001316"
     CDS             78..599
                     /locus_tag="VC122_001316"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000237805.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HEP5641238.1"
                     /translation="MTKDRQIKKTKTAIYSAFLALLQKKEYSKITVRDMITLANVGRS
                     TFYAHYESKEMLLKELCEELFHHLFRQKRNVTFEDYLVHILKHFEQNKDSIATLLLSN
                     DPYFLLRFKNELEHDVYPNLRCKYIDKTTIPEVFLKQFVLSSFIETLKWWLHQRQRMS
                     ANELLKYYLELIK"
     gene            814..1332
                     /gene="rimM"
                     /locus_tag="VC122_001317"
     CDS             814..1332
                     /gene="rimM"
                     /locus_tag="VC122_001317"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016465641.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome maturation factor RimM"
                     /protein_id="HEP5641239.1"
                     /translation="MEYFNVGKIVNTQGLQGEMRVLSVSDFAEERFKKGSQLALFDDK
                     DQFVQEVTIVSHRKQKHFDIIKLKDMYHINAIEKYKGYTLKVSKANQGDLQEGEFYYH
                     QIIGMAVYEKDRLIGYVKEILQPGANDVWVVKRQGKRDLLLPYIPPVVLNVDVPNKRV
                     DVELMEGLDDED"
     gene            1322..2053
                     /gene="trmD"
                     /locus_tag="VC122_001318"
     CDS             1322..2053
                     /gene="trmD"
                     /locus_tag="VC122_001318"
                     /EC_number="2.1.1.228"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014407493.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (guanosine(37)-N1)-methyltransferase TrmD"
                     /protein_id="HEP5641240.1"
                     /translation="MKIDILTLFPEMFAPLEHSIVGKAKEKGLLDIHYHNFRDYAEKA
                     RHVDDEPYGGGQGMLLRAQPIFDTIEQIEAKKPRIILLDPAGKPFTQAYAEELALEEE
                     LIFICGHYEGYDERIKTLVTDEISLGDFVLTGGELAAMTMVDATVRLIPQVLGKESSH
                     QDDSFSSGLLEYPQYTRPYDYRGMTVPDVLMSGHHERIRLWRLEESLRKTYLRRPDLL
                     ERYDFSEEERKLLDKIKEALGQGED"
     gene            2053..3045
                     /locus_tag="VC122_001319"
     CDS             2053..3045
                     /locus_tag="VC122_001319"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014407494.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HEP5641241.1"
                     /translation="MKDKAYDITIIGGGPIGLFAAFYAGLRGVTVKIIESLSELGGQP
                     AILYPEKMIYDIPAYPSLTGVELTENLIKQLNRFEDRITICLKEEVLTFDKVKGGFSI
                     RTNKAEHFSKAIIIACGNGAFAPRTLGLESEENFADHNLFYNVHQLDQFAGQKVVICG
                     GGDSAVDWALALEDIAESVTVVHRRDAFRAHEHSVELLTTSTVNLLTPYVPKALKGIG
                     NLAEKLVIQKVKEDEVLELELDSLIVSFGFSTSNKNLKNWNLDYKRSSITVSPLFQTS
                     QEGIFAIGDAAAYNGKVDLIATGFGEAPTAVNQAINYIYPDRDNRVVHSTSLID"
     gene            3224..4282
                     /locus_tag="VC122_001320"
     CDS             3224..4282
                     /locus_tag="VC122_001320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002990218.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit IIC"
                     /protein_id="HEP5641242.1"
                     /translation="MTTTDKETFSSFMNKVLAGTAIAIVVALIPNAILATFLKPLLPN
                     MAAAEFLHIVQVFQFFTPIMAGFLIGQQFKFNPMQQLAVGGAAYIGSGAWAYTEVIQK
                     GVATGTFQLRGIGDLINMMITASLAVLAVKYFGNKFGSLTIILLPITIGTGVGYIGWK
                     FLPYVSYVTTLIGQGINSFTTLQPILMSILIAVAFSLIIVSPISTVAIGLAIGLNGMA
                     AGAASMGIASTAAVLVWATLKVNKSGVPIAIALGAMKMMMPNFLKHPIMAIPMVFTAA
                     ISSLTVPLFNLVGTPASSGFGLVGAVGPIASLAGGSSILIIILAWIIVPFAVAFAAHK
                     VSKDILKLYKEDIFVFEG"
     gene            4295..5218
                     /locus_tag="VC122_001321"
     CDS             4295..5218
                     /locus_tag="VC122_001321"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000964166.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-dehydropantoate 2-reductase"
                     /protein_id="HEP5641243.1"
                     /translation="MLVYIAGSGAMGCRFGYQISKTNNDVILLDNWEDHINAIKENGL
                     VVTGDVEETVKLPIMKPTEATQEADLIILFTKAMQLPQMLQDIKGIIGKETKVLCLLN
                     GLGHEDVIRQYIPEHNILMGVTVWTAGLEGPGRAHLQGVGALNLQSMDPNNQDAGHQV
                     ADLLNKANLNATYDENVVPNIWRKACVNGTMNSTCALLDCTIGELFASEDGLKMVKEI
                     IHEFVIVGQAEGVELNEEEITQYVMDTSVKAAHHYPSMHQDLVQNHRLTEIDFINGAV
                     NTKGEKLGINTPYCRMITELVHAKEAVLNIQ"
     gene            5474..6187
                     /locus_tag="VC122_001322"
     CDS             5474..6187
                     /locus_tag="VC122_001322"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003048118.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DeoR/GlpR transcriptional regulator"
                     /protein_id="HEP5641244.1"
                     /translation="MAKITEENYVSLEDLMQLLNSSESTIRRDLGELEQEGRLHRVHG
                     GAELFHSLQEELSNQEKSVKNSHIKKTIAQRASQFIYDNDVIFIDAGTTTEFLLPFLQ
                     AKNLTVVTNSIHHAARLVELSIETIIVGGYVKQTTDASIGNVALEQIRQMNFDKAFLG
                     MNGIDDSYLTTPDMEEAVIKKAVLSNAKLAYILVDGTKIGQVSFVKVAPINDVTIITL
                     GGSASILKQIKEKAKVIEL"
     gene            6184..7095
                     /gene="pfkB"
                     /locus_tag="VC122_001323"
     CDS             6184..7095
                     /gene="pfkB"
                     /locus_tag="VC122_001323"
                     /EC_number="2.7.1.56"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015016911.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="1-phosphofructokinase"
                     /protein_id="HEP5641245.1"
                     /translation="MIYTVTLNPSIDFIVRIDQINLGSVNRMASDDKFAGGKGINVSR
                     ILQRLDIASTATGFLGGFTGRFIEESLSAEGVKTDFVKGDQDTRINVKIKSQEETEIN
                     GQGPVISREQLEDLKTKLSQLTAEDTVVFAGSAPANLGNAVYKELFPLVRQSGAQVVC
                     DFEGQTLIDALTYNPLLVKPNNHELEAIFGTILTSLDDVETYARRLLEMGAQNVIISM
                     AGDGALLVTKEATYFAKPIKGEVKNSVGAGDSMVAGFTGEFMKSQNPIEALKWGVACG
                     TATAFSDDLATIAFIKETYHKVEVEKR"
     gene            7092..9038
                     /locus_tag="VC122_001324"
     CDS             7092..9038
                     /locus_tag="VC122_001324"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003050270.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HEP5641246.1"
                     /translation="MKIQDLLRKDIMILDLQAISKEVAIDEMITKLVEKDIVHDFDVF
                     KKSIMTREEQTSTGLGDGIAMPHSKNIVVDKPAVLFAKSNKGVDYKALDGQPTDLFFM
                     IAAPQGANDTHLAALAELSQYLLKDGFADKLRAAATPEAVIAVFDEASTAKEEVVAPT
                     SGQDFIVAVTACPTGIAHTYMAEEALKKQAAEMGVAIKVETNGASGVANRLTAEDIQR
                     AKGVIVAADKAVEMDRFDGKQFIARPVADGIKKSQELISLILNNEGNTYHAKNGKSET
                     AVSTEKTSLGGAFYKHLMGGVSQMLPFVIGGGIMIALAFLLDNMLGVPNDQLGSLGSY
                     HEIAAIFMNIGGAAFSFMLPVLAGYIAYSIAEKPGLVAGFVAGAIASNGLAFGKVPFA
                     AGGEVSLGLTGVPSGFLGALVGGFLAGGVILALRKLLAGLPRSLEGVKSILLYPLLGV
                     LVTGFLMLFVNIPMAAINTALNDFLQGLSGSSAVLMGLLVGGMMAVDMGGPVNKAAYV
                     FGTGTLAATVANGGSVVMAAVMAGGMVPPLAVFVATLLFKDKFTKEERESGLTNIVMG
                     LSFITEGAIPFGAADPARAIPSFIAGSALTGALVGLAGIKLMAPHGGIFVIALTSNPI
                     LYLVFVVIGALVSGILFGALRKKA"
     gene            9131..9730
                     /locus_tag="VC122_001325"
     CDS             9131..9730
                     /locus_tag="VC122_001325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011284690.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 73 protein"
                     /protein_id="HEP5641247.1"
                     /translation="MKVKKRRRRAKSSVNRLVLGLVLLNLIVSMWTLKLGNQRLAPYA
                     DHETLTFVRKISHAAQSVAQKKQLYSSVMMAQAILESNNGKSQLSQKPYYNFFGIKGS
                     YKERSVIFPTLEDDGQGNLYQIDAAFRSYGSLTACFLDYARVLNDPLYDKTHKKFWSH
                     YQDATATLTGTYATDTTYNTKLNELIEWYQLTNFDGLMK"
     gene            9882..10589
                     /locus_tag="VC122_001326"
     CDS             9882..10589
                     /locus_tag="VC122_001326"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002990206.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 73 protein"
                     /protein_id="HEP5641248.1"
                     /translation="MTKKKGKLVLISLFVLAACLGAYSAMRQSHKTSNVSAETIASSS
                     TRHFIDEIGPTASTIGQERDLYASVMIAQAILESSNGKSSLSQAPYYNFFGIKGAYNG
                     SSVTMSTWEDDGNGNTYTIDQAFRAYPSIADSLNDYADLLSSSTYIGARKSNTLSYQD
                     ATAALTGLYATDTSYNLKLNNIIATYGLTAYDVANSSAQETGLATSGYVWNEYRRNYT
                     DAETLAVDEAWAKRMTY"
     gene            complement(10646..>10718)
                     /locus_tag="VC122_001327"
     CDS             complement(10646..>10718)
                     /locus_tag="VC122_001327"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611514.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="IS3 family transposase"
                     /protein_id="HEP5641249.1"
                     /translation="NNKRIKIKRKGLSPLQYRTKSFT"
BASE COUNT         3317 a         1751 c         2308 g         3342 t
ORIGIN      
        1 taaaagttaa aatacaatta ctataaccaa aaatgagcac ctgttcaata gtgaacaaca
       61 tttagaaagt gtccgatatg acaaaagaca gacaaatcaa aaaaacaaaa acagctatct
      121 atagtgcttt tcttgcttta ttacaaaaaa aagaatattc gaaaataact gttcgagata
      181 tgattacact tgctaatgta gggagatcaa ccttttatgc acattatgaa agcaaagaaa
      241 tgttactaaa agaactctgt gaagaacttt ttcatcattt gtttaggcaa aaaagaaatg
      301 tgacttttga ggactatttg gtacatattt taaagcattt tgaacaaaat aaagacagca
      361 ttgcgacgtt gttactttct aatgatccct attttttatt aagatttaaa aacgaactag
      421 aacatgatgt ttaccctaac ttgcgctgca agtatatcga caaaacgact attccagaag
      481 tattcctgaa acaatttgtt ctatctagtt ttatagaaac cttaaaatgg tggctccatc
      541 aaaggcaaag gatgtctgca aatgaactat taaagtatta tttagagctc ataaaataat
      601 gactagtgag tcatcttgcc aaattcaatt ttttaataca gacggatttt aacaatttaa
      661 cagagaaaga caaaactgta agtgcaaaat agactataac gtgataaaat aataggtaat
      721 ttggttctga aaaagaagtg tctatcaaac tttagaatca agagaggtcg ctaaagtgac
      781 gtgagataaa gagttaacga tttaggagct accatggaat attttaatgt tggaaaaatt
      841 gtcaacacgc aaggcctgca aggagagatg cgcgtgttgt ctgtcagtga ttttgctgag
      901 gagcgcttta agaagggatc gcaattggct ttgtttgatg acaaagacca gtttgttcaa
      961 gaagtgacaa ttgttagtca ccgtaaacaa aagcattttg atattatcaa attgaaagac
     1021 atgtatcata ttaatgcgat tgaaaaatat aagggctata cgttaaaagt gtcaaaagct
     1081 aatcaaggag acctgcaaga aggagaattt tactaccatc agattatcgg tatggctgtt
     1141 tatgaaaagg accggttgat tggctatgtc aaggaaatct tgcagccagg tgctaatgat
     1201 gtttgggtag tgaaacgcca aggaaaacga gacttgctct taccatatat tcccccagta
     1261 gttcttaacg tagatgttcc taacaagcgc gtagatgttg aattgatgga aggcttagac
     1321 gatgaagatt gatattttaa ccctttttcc tgaaatgttt gcccccctag aacattccat
     1381 tgtaggcaaa gcaaaagaaa aaggattact tgacattcac taccataact ttcgagatta
     1441 tgctgaaaaa gctcgtcatg tggatgacga accctatggt gggggccaag gcatgttatt
     1501 gagagcacag cccatttttg acaccattga acagattgaa gccaaaaaac caaggattat
     1561 tcttttagat ccagcaggta aaccctttac tcaggcttat gctgaagaat tagctcttga
     1621 ggaagagtta atttttattt gtggtcatta tgaaggttat gatgagcgga ttaagacctt
     1681 ggtgacggat gaaatttcat taggtgactt tgtattaaca ggaggtgagt tggcggccat
     1741 gactatggtg gatgcgactg tcaggttaat tccacaggta cttgggaaag aaagtagcca
     1801 tcaggacgat tctttttcat caggactttt agaataccct caatacacaa gaccttatga
     1861 ttatcgtggt atgacagttc cagatgttct aatgagtggt catcacgaac gtattcgctt
     1921 gtggcgtcta gaagaaagtc tcagaaaaac ctatttgaga aggccagatt tgttagagcg
     1981 gtacgatttt tccgaggaag agcgcaagct attggataag attaaagaag cattgggtca
     2041 aggagaagat tagtgaaaga taaagcatat gatattacga ttattggtgg tggtccaata
     2101 ggtttatttg cagcctttta tgcaggtctt aggggcgtaa cagtaaagat tattgagagt
     2161 ttgtcagaac ttggtggaca gcctgccatt ctttatcctg agaaaatgat ttatgatatt
     2221 ccagcttatc catctttaac aggtgttgag ttgacagaaa atttaataaa acaattaaac
     2281 cgttttgaag atcgtataac catttgttta aaagaagaag tattaacctt tgacaaggtt
     2341 aaaggtggtt tttcgattag gactaataag gcagagcatt tttcaaaagc cattatcatt
     2401 gcttgtggaa atggcgcttt tgcaccaaga actttagggt tagaaagtga ggaaaacttt
     2461 gctgatcata acctttttta taatgttcac cagttggacc agtttgcggg acaaaaagtt
     2521 gtgatttgtg gtggtggaga ttcagcagtt gattgggctt tggctttaga agatattgct
     2581 gagagtgtga cggttgtgca ccgtcgtgat gctttcagag cacatgaaca cagtgtggaa
     2641 ttattgacaa catcaacggt aaacctctta acaccttatg tgccaaaagc attgaaagga
     2701 attggaaacc tggctgaaaa attagttatt caaaaagtga aagaagatga agtgttagaa
     2761 cttgaattgg acagtcttat tgttagtttt ggtttttcaa cttctaataa gaatttgaaa
     2821 aattggaatc tcgactataa gcgttcaagt attactgttt caccactttt tcaaacaagt
     2881 caagaaggta tttttgccat tggtgatgca gccgcttata atgggaaagt ggatttgatt
     2941 gctactggtt ttggcgaagc accaacagca gttaatcagg ccattaacta tatttatcca
     3001 gatcgagata accgtgtggt gcattcaacc tctttaattg actaagggag tcggaagttt
     3061 caagagcagt agcaacagct aatattcagt ttgtcaggag aggacaaatg gttttttacc
     3121 taagtcttcg tgacaagttt tatactttgt gataaaatag tcattgtgaa aaaagtgtga
     3181 tggttttcac agattaacac attacaatga aaaggggtct atcatgacaa caacagataa
     3241 agagacattt agttctttta tgaataaggt cttggctggt acagcgattg ctatcgttgt
     3301 ggcactcatt ccaaatgcta ttttagcgac tttcttaaaa ccgcttttgc caaacatggc
     3361 agctgctgaa tttttacaca ttgtgcaagt cttccaattc ttcacgccga ttatggctgg
     3421 tttcttgatt gggcaacaat ttaagtttaa tcctatgcaa cagttggctg ttggtggagc
     3481 tgcttatatc ggttctgggg cttgggctta tacagaagtc attcaaaaag gtgtggcgac
     3541 aggaacattc caacttagag gtattgggga cttaatcaat atgatgatta cagctagcct
     3601 tgcggtttta gctgtgaaat attttgggaa taaatttggc tctttaacca ttatcttgtt
     3661 acctatcact ataggaactg gcgtaggtta tattggttgg aaatttttgc cttatgtgtc
     3721 ttacgtgaca accctgatcg gacaaggtat taattctttt acaaccttac aacctatttt
     3781 aatgtctatt ttaattgcag ttgctttctc gcttatcatt gttagtccga tttcaactgt
     3841 tgcaatcggt ttagctattg ggcttaatgg aatggctgcg ggagcagctt ctatgggaat
     3901 tgcatcgaca gcagcagtct tagtgtgggc gacgcttaaa gttaataaat ctggtgtacc
     3961 aattgctatc gcacttgggg ctatgaaaat gatgatgcca aacttcttga aacatcctat
     4021 catggctatt ccgatggtgt tcactgcagc aattagctca ttaacagtac cattgtttaa
     4081 tcttgttgga acaccagctt catctggttt tggcttagtt ggtgcagtag gtccgattgc
     4141 ttctttagca ggtggtagct caatacttat tattatcctt gcttggatca ttgttccgtt
     4201 tgcggttgct tttgcggcgc ataaggtttc taaagatatt ctaaaacttt ataaagaaga
     4261 cattttcgtc tttgaaggct aataggagga aaatatgtta gtttatattg ctggctcagg
     4321 agccatgggg tgccgttttg gttaccaaat ttcaaaaaca aataacgatg tgattttgtt
     4381 agataactgg gaagatcata tcaatgccat taaagaaaac ggtttagtgg tgacagggga
     4441 tgtggaagaa acagttaagc ttcctattat gaaaccgact gaagccactc aagaagccga
     4501 tttgattatc ttgtttacaa aagcaatgca gttgccacaa atgcttcaag acattaaagg
     4561 cattattgga aaagaaacca aagtgctttg ccttttaaat ggtcttggac acgaagacgt
     4621 tattcgtcag tacattccag agcacaacat tttgatgggg gtcactgtct ggactgctgg
     4681 tctagaagga ccaggtcgtg ctcatcttca aggagttggt gctcttaact tacaaagtat
     4741 ggatccaaac aatcaagatg cgggacatca agtagctgac ttgttaaata aagctaactt
     4801 aaatgcgact tatgatgaga atgttgtgcc aaacatctgg cgaaaagctt gcgtgaatgg
     4861 taccatgaat tcgacttgtg ctcttttaga ttgtacgatt ggcgaattat ttgccagtga
     4921 agatggtctc aaaatggtaa aagaaatcat ccatgagttt gttatcgttg gtcaagcaga
     4981 aggtgtcgaa ctaaacgaag aagaaatcac acaatatgta atggatacat ctgttaaagc
     5041 tgctcaccat tacccatcaa tgcaccaaga ccttgtgcaa aatcaccgat tgactgaaat
     5101 tgattttatc aatggtgcag tcaataccaa aggtgaaaag cttggtatca atactccata
     5161 ctgccgtatg attacagaac tagttcacgc taaagaagca gtgttgaaca tccaataaac
     5221 gctatcaaat cgttatactc aatattatgg ctaaagcttt aatattgagt tttttgttat
     5281 gctgatagta tttttcttag gcgtcattat ttgtgagcct agattatgag ttaataaaag
     5341 aaataataat tggtagaatt tgatagattt tgaaaggtta tgcttgactt ttagttataa
     5401 aatagtataa taattgtaaa cgatttcagt taggggtggt tctatcttaa aatcaaaacg
     5461 aaaacaatta atcatggcaa agatcactga agaaaactat gtttctttag aggatttaat
     5521 gcaattactc aactcctctg agtctactat ccgtagagat ctaggagagt tggagcaaga
     5581 aggacggcta catcgtgttc atggaggtgc tgaacttttt cactctttac aagaggaact
     5641 ttctaaccaa gaaaagtctg tcaaaaacag tcatattaaa aaaactattg cgcaaagagc
     5701 ctctcagttt atctatgaca acgatgtgat ctttattgat gctgggacaa cgactgaatt
     5761 tttattacct tttttacaag ctaaaaattt aactgtagtt actaattcta ttcatcatgc
     5821 tgctagatta gttgaactct ctattgagac gattattgta ggtggttatg tcaaacaaac
     5881 gaccgatgcc agtattggta atgtagcttt ggaacagatt aggcagatga attttgacaa
     5941 ggcatttctt ggaatgaatg gtattgatga ttcttacctg acgactcctg atatggaaga
     6001 agcagttatt aaaaaagcgg ttctgtcaaa tgcaaaatta gcttacattt tagtagatgg
     6061 cactaaaatt ggtcaggtat cgtttgtaaa agtagctcct attaatgatg tgactattat
     6121 tacattagga ggatcagcaa gtattcttaa acaaataaag gaaaaggcaa aggtgattga
     6181 attatgattt ataccgtgac cttaaaccct tctattgact ttattgtcag aattgaccaa
     6241 ataaatcttg ggtcagtgaa tcgcatggcg agtgatgata agtttgctgg aggtaaaggc
     6301 attaatgtga gtcgcatctt gcaacgttta gacattgcta gcacggcgac aggattcttg
     6361 ggtggtttta caggacgttt tatcgaagaa agtttgagtg cagaaggcgt taaaactgat
     6421 tttgtaaaag gtgatcaaga tactcgaatc aatgttaaaa tcaagtcaca agaagagact
     6481 gaaataaatg gtcaaggacc tgtcattagt cgggaacaac tagaggattt gaaaactaag
     6541 ctttcccagc taacagcaga ggatacagtt gtctttgcag gttcagcgcc agctaatctt
     6601 ggcaatgctg tttataagga attatttcct ttagttaggc aaagcggtgc tcaggtggtt
     6661 tgtgattttg aaggacagac tttaatagat gcgcttacat ataatcctct gttggttaaa
     6721 cctaataatc atgaactaga agctattttt ggtacgattc tgactagtct tgatgatgtt
     6781 gaaacctatg cccgtcgtct tttagagatg ggtgctcaaa acgtcatcat ttcaatggct
     6841 ggtgatggtg ctttgctggt tactaaagaa gcgacttatt ttgctaaacc gattaaggga
     6901 gaagttaaaa attctgtcgg tgctggtgat tcgatggttg ctggctttac aggcgaattt
     6961 atgaagagtc aaaatccaat agaagcctta aaatggggcg tggcttgtgg tacagcgacg
     7021 gctttctctg atgacttggc gacaattgca tttattaagg aaacatatca taaagttgag
     7081 gtagaaaaac gatgaaaatt caagatttat tgagaaaaga tattatgatt ctcgatttac
     7141 aggctatttc taaagaagtt gccattgacg agatgattac taaattagtt gaaaaagata
     7201 ttgtacatga ttttgatgtc tttaaaaaga gtatcatgac acgtgaagaa caaacatcaa
     7261 cagggcttgg tgatgggatt gccatgcctc attctaaaaa tattgtagta gataaaccag
     7321 cggtcttgtt tgcgaaatca aataaaggtg tggattataa ggctttagat ggtcaaccaa
     7381 cggatctctt ctttatgatt gctgcgcctc aaggagcaaa tgatactcac ttggctgcgc
     7441 tagcagaatt atcacaatac cttttgaaag acggctttgc tgataaactt cgtgctgcag
     7501 cgacaccaga agcagtcatt gctgtttttg atgaagcgtc aacagctaaa gaagaagtgg
     7561 ttgctccaac aagtggtcaa gactttatcg ttgctgttac agcttgtccg acaggaattg
     7621 ctcacactta tatggcagaa gaggctttga aaaaacaagc tgcagaaatg ggagtagcta
     7681 ttaaagttga aacaaacggt gcttctggtg tggctaatcg tttaaccgct gaagatattc
     7741 agagagctaa aggagttatt gttgcagctg ataaagcggt tgaaatggac cgttttgatg
     7801 gtaaacaatt cattgctcgc cctgttgcag atggtatcaa gaaaagtcag gaattgattt
     7861 ctttgatttt aaacaatgaa ggaaacactt atcatgctaa aaatggaaaa tctgaaacag
     7921 cagtatcaac tgagaaaaca agtttaggcg gtgctttcta taaacacttg atgggcggtg
     7981 tctcacaaat gttgccattt gttattggtg gcgggattat gattgctcta gcatttttat
     8041 tggataacat gcttggtgtg ccaaatgatc agcttggaag tcttggatcc tatcatgaaa
     8101 tagcagctat ttttatgaac attggtggag cagccttttc cttcatgtta ccagtactag
     8161 caggttatat cgcttattct attgctgaaa aaccgggctt agtagctggt tttgtggcag
     8221 gtgccattgc ctcaaatggt cttgcttttg gtaaggtacc atttgcagca ggaggagaag
     8281 tgagcttggg attaacgggc gtgccatcag gtttccttgg ggcgcttgtg ggtggtttcc
     8341 ttgccggtgg tgttattctt gcccttcgta aattgttggc aggcctacca cgttctctag
     8401 aaggggttaa atctatcctc ctttacccat tacttggcgt ccttgtcact ggtttcttga
     8461 tgctttttgt caatattcca atggcagcta ttaacacagc tcttaatgat ttcttacaag
     8521 gtctttcagg aagctctgct gtccttatgg gacttcttgt tggtggaatg atggctgttg
     8581 acatgggtgg tcctgttaat aaggcagctt atgtttttgg tacaggaact ttagcagcta
     8641 ctgtggcaaa tggtggttcg gtcgttatgg ccgctgttat ggcaggtggt atggtacctc
     8701 ctcttgctgt ttttgtagcc actctcttgt ttaaagataa atttaccaaa gaagaacgcg
     8761 aatcaggttt gaccaacatt gtcatgggac tttctttcat cactgaagga gcaatcccat
     8821 ttggcgcagc tgacccagcg cgtgcaattc ctagctttat tgctggttct gccttgacag
     8881 gagctcttgt tggtttggct ggcattaaat taatggcgcc acatggtggt atcttcgtaa
     8941 tcgctttaac aagcaatcca atcttatatc ttgtctttgt tgtgattggt gcccttgtat
     9001 caggtatctt atttggcgct cttcgcaaaa aagcctaaaa ggtaaagaac ttttttctta
     9061 ctaaaactag aatctgtcaa ggttctagtt ttttgctata ctagagttaa aggataagaa
     9121 atgaggacct ttgaaggtga aaaaaaggag aagaagggct aaatcatcag taaatcgact
     9181 tgtcttaggg ttagtattac ttaatctgat tgttagtatg tggacactaa aattgggaaa
     9241 ccagcgatta gctccgtatg cggatcacga aactctgaca tttgtcagga agattagtca
     9301 tgctgctcaa tcagtcgctc aaaagaaaca gttatacagt tcggtgatga tggctcaggc
     9361 cattttagaa tccaataatg gcaagtcaca actaagtcaa aaaccttatt ataatttttt
     9421 cgggattaag gggagctata aagaacggtc agtcattttt ccaactttag aagacgatgg
     9481 acaagggaat ctttatcaaa ttgatgctgc ttttcgttcc tatgggagcc tgacagcttg
     9541 ttttttagat tacgcaagag ttttgaatga tccactttat gacaaaaccc acaaaaagtt
     9601 ttggtctcat tatcaagatg ctactgcaac cttaacaggc acttacgcca ctgatacaac
     9661 ttacaatacc aaattaaatg aattgattga atggtatcaa ctcactaatt ttgatggtct
     9721 aatgaaatag ttatccacaa ttttaaaaaa aatcagaaga aatatgtcga tttcaagaca
     9781 aaagttatca atctcattaa attatctgtg gaaaagttaa tttaaacgtt cgtgtgctat
     9841 aatagaaaca tagaaaatgt ataaaaaagg atatgtgaat aatgacaaaa aagaaaggta
     9901 agcttgtact tatcagtctt tttgttctag cagcttgttt aggagcttat agtgcaatga
     9961 gacaatctca taaaacttca aacgtgtcag ctgagactat cgccagttct tcaacccgac
    10021 attttattga tgaaattggt ccaactgcta gtactattgg tcaagaacgt gatctttatg
    10081 cgtctgttat gatagcacaa gcgattttag aatcaagtaa tggtaaatca agtctaagtc
    10141 aagcacctta ttataatttt ttcggtatta agggtgccta taacggttct tctgtgacga
    10201 tgtcaacttg ggaagatgat ggtaatggca atacctatac gattgatcaa gcctttagag
    10261 cttatccaag tattgcagac tcccttaatg actatgcaga cttgctaagt tctagcactt
    10321 atataggtgc tagaaaatca aacacgctat cgtatcaaga tgctacagca gctctaactg
    10381 gcttatacgc tacagacacc agttataact taaaactaaa taatattatt gcaacctatg
    10441 gtttgacggc ttatgatgtt gctaatagct cagctcaaga aactggtcta gcaacttcgg
    10501 gttatgtttg gaatgagtat cgtcgtaatt acactgatgc ggagacccta gcagttgatg
    10561 aggcttgggc taaacgcatg acttattaat gtaacactaa caacaatctg cttgcactga
    10621 ccccaaaaaa gctggatact ctattttaag taaaggattt agttctgtat tgtagaggac
    10681 taagtccttt tcgttttatt ttgatacgtt tattgttg
//
LOCUS       DATXIG010000047         9844 bp    DNA     linear   BCT 28-DEC-2023
DEFINITION  TPA_asm: Streptococcus pyogenes strain MGAS11113
            SAMN08555799-rid19682583.denovo.047, whole genome shotgun sequence.
ACCESSION   DATXIG010000047 DATXIG010000000
VERSION     DATXIG010000047.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN08555799
            Sequence Read Archive: SRR6740495
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pyogenes
  ORGANISM  Streptococcus pyogenes
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 9844)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9844)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-OCT-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-OCT-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001918058.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001918058.2
            Genome Coverage       :: 368x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/12/2023 11:23:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 1,806
            CDSs (total)                      :: 1,769
            Genes (coding)                    :: 1,722
            CDSs (with protein)               :: 1,722
            Genes (RNA)                       :: 37
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1, 1 (5S, 23S)
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 47
            CDSs (without protein)            :: 47
            Pseudo Genes (ambiguous residues) :: 0 of 47
            Pseudo Genes (frameshifted)       :: 30 of 47
            Pseudo Genes (incomplete)         :: 20 of 47
            Pseudo Genes (internal stop)      :: 9 of 47
            Pseudo Genes (multiple problems)  :: 11 of 47
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9844
                     /organism="Streptococcus pyogenes"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN08555799-rid19682583.denovo.047"
                     /strain="MGAS11113"
                     /serotype="Emm28"
                     /isolation_source="normally sterile site"
                     /host="Homo sapiens"
                     /db_xref="taxon:1314"
                     /country="Finland"
                     /collection_date="2002"
                     /collected_by="National Public Health Laboratory"
     gene            <1..218
                     /gene="grab"
                     /locus_tag="VC122_001328"
     CDS             <1..218
                     /gene="grab"
                     /locus_tag="VC122_001328"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010922409.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="G-related alpha2-macroglobulin-binding protein
                     GRAB"
                     /protein_id="HEP5641250.1"
                     /translation="ASDAWEKAATPTALDVKKTKDTTPVVKKEERQNVNTLPTTGEES
                     NPFFTAAALAITVSTGALVVSSKCKEN"
     gene            456..998
                     /locus_tag="VC122_001329"
     CDS             456..998
                     /locus_tag="VC122_001329"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002984095.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HEP5641251.1"
                     /translation="MDIWTKLAVFAFFETPKVILRPFRYEDHWDFYSMVNDTKNLYYV
                     FPEQKTKAASDYLLVHSFIKFPLGQWAIEDKATHQVIGSIRIEHYDAKTRCADIGYFL
                     NYAFWGQGIMTEVVIKLVYLSFHEFGLKTLRIITHLENKASQKVAKKAGFQLKTCFKG
                     SDRNTHKICIYKMYQLTNDR"
     gene            1007..2290
                     /locus_tag="VC122_001330"
     CDS             1007..2290
                     /locus_tag="VC122_001330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008090728.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CBS domain-containing protein"
                     /protein_id="HEP5641252.1"
                     /translation="MSKHQDILDYLEKLAIGKKVSVRSISNHLKVSDGTAYRAIKEAE
                     NRGIVETKPRSGTVRIEKKGRVRIDRLTYSEIARISDSEVLAGHAGLGHEFSRFSIGA
                     MTQQNIRRYLVKGGLLIVGDRETIQLLALENHNAILVTGGFPVSKRVIEMANNQRIPV
                     MVTHYDTFTVATMINHALSNIRIKTDLKTVEQVMIPITDYGYLCENSSVEEFNTLIKK
                     TRQVRFPVLDYKRKVIGVVSMRDVVDQLPTTKLTKVMSKNPITARPNTSLANISQKMI
                     FEDLNMLPVTDEENNLLGMITRRQAMENLPNHQPNNPYTYSEQILSNLEETVDYYQVV
                     VEPTMIDSAGNMSNGVISEFLKEISIRALTKKHQKNIIIEQMMVYFLHAIQIEDELKI
                     YPKIITENRRSSTIDIEIFVDDQVIAKAIITTKIN"
     gene            2306..3166
                     /locus_tag="VC122_001331"
     CDS             2306..3166
                     /locus_tag="VC122_001331"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013903404.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methionyl aminopeptidase"
                     /protein_id="HEP5641253.1"
                     /translation="MITLKSAREIEAMDRAGDFLAGIHIGLRDIIKPGVDMWEVEAYV
                     RRRCKEDNVLPLQIGVDGHMMDYPYATCCGLNDEVAHAFPRHYILKEGDLLKVDMVLS
                     EPLDKSIVDVAALDFDNVPEMKKWTRSYTGGLADSCWAYAVGTPSDEIKQLMDVTKEA
                     MYRGIEKAVIGNRIGDIGAAVQEYAESFGYGVVRDLVGHGVGPTMHEEPMVPNYGTAG
                     RGLRLKEGMVLTVEPMINTGTWEIDTDIKTGWAHKTLDGGLSCQYEHQFVITKDGPVI
                     LTSQGEERTY"
     gene            3168..4133
                     /locus_tag="VC122_001332"
     CDS             3168..4133
                     /locus_tag="VC122_001332"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014407653.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YihY/virulence factor BrkB family protein"
                     /protein_id="HEP5641254.1"
                     /translation="MAEKKWFDKVLSKWQYEPIQVFMRHLQSAEMDLSAIAVAYYLIL
                     TAFPLIVIAANIFPYLNIDIADLLRLMKQNLPKDIFRPASAIVENIFSKPSGSVLGVA
                     TLTGLWTMSRSLTSLQKAINKAYGASQHRDFFIGHLVGLLTSLIILFLLAFALIFSTF
                     SKAAIQVLDKHYHLSDNITTIFLLLIQPITVLIIFVGLMLLYFLLPNVKIKKIRYILP
                     GTLFTSFVMTFLSNLVGNYVVYNVERMVDIKMFGSVMIFIIMLWFIFLARILILGAIF
                     NATYQEMSLGKLEGRSGDMIAILKKTLGNDSDLSPSQSIEDSHTD"
     gene            4244..5527
                     /gene="aroA"
                     /locus_tag="VC122_001333"
     CDS             4244..5527
                     /gene="aroA"
                     /locus_tag="VC122_001333"
                     /EC_number="2.5.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012679248.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-phosphoshikimate 1-carboxyvinyltransferase"
                     /protein_id="HEP5641255.1"
                     /translation="MKLRTNAGPLQGTIQVPGDKSISHRAVILGAVAKGETRVKGLLK
                     GEDVLSTIQAFRNLGVRIEEKDDQLVIEGQGFQGLTAPCQTLNMGNSGTSMRLIAGLL
                     AGQPFSVKMIGDESLSKRPMDRIVYPLKQMGVEISGETDRQFPPLQLQGNRNLQPITY
                     TLPISSAQVKSAILLAALQAKGTTQVVEKEITRNHTEEMIQQFGGRLIVDGKRITLVG
                     PQQLTAQEITVPGDISSAAFWLVAGLIIPGSELLLKNVGVNPTRTGILEVVEKMGAQI
                     VYEDMNKKEQVTSIRVVYSHLKGTIISGGLIPRLIDELPIIALLATQAQGTTCIKDAQ
                     ELRVKETDRIQVVTDILNSMGANIKATADGMIIKGPTVLYGANTSTYGDHRIGMMTAI
                     AALLVKQGQVHLDKEEAIMTSYPTFFKDLERLCHD"
     gene            5520..6011
                     /locus_tag="VC122_001334"
     CDS             5520..6011
                     /locus_tag="VC122_001334"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010922406.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="shikimate kinase"
                     /protein_id="HEP5641256.1"
                     /translation="MTKVLLGFMGVGKTTVSKHLSMHCKDMDAIIEAKIGMSIAAFFE
                     QHGEIAFRTIESQVLKDLLFANDNSIIVTGGGVVVLQENRQLLRKNHQHNILLVASFE
                     TLYQRLKHDKKSQRPLFLKYSKEAFYEFYQQRMVFYEGLSDLVIRVDHRTPEEVANII
                     EGY"
     gene            6219..7670
                     /locus_tag="VC122_001335"
     CDS             6219..7670
                     /locus_tag="VC122_001335"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002984109.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LCP family protein"
                     /protein_id="HEP5641257.1"
                     /translation="MTKYPMGGLSHHEELRYFYLLRNLSYLSENEKKEFAFLKSKLEI
                     GRAYAPSKQHYRKSKRQEPYFEDDYYNDYSPNDLLEDDDVNHDSSFVPYGLPIYPKED
                     RYLNKKTKLTARRPIDTPQPIDEDDAFLTESVARCALPRSQKRKHKKKGCMKWFFNIL
                     GLLLMTVLMGLGLMFAKGVFDISTNKANYKPAVSQAFDGQETQDGTNILVLGSDQRVT
                     QGSTDARTDTIMVVNVGNHAKKIKMVSFMRDTLINIPGYSYNDNSYDLKLNSAFNLGE
                     QEDHHGAEYVRRALKHNFDIDIKYYVMVDFETFAEAIDTLFPNGVKIDAKFATVGGVA
                     VDSVEVPDDLRMKNGVVPNQTIEVGEQRMDGRTLLNYARFRKDDEGDFGRTVRQQQVM
                     SAVMSQIKDPTKLFTGSAAIGKIYALTSTNVSFPFVVKNGVSVLGSGKNGVEHVTIPE
                     NGDWVDEYDMYGGQALYIDFDKYQKTLAKLGLR"
     gene            7752..9386
                     /gene="rlmD"
                     /locus_tag="VC122_001336"
     CDS             7752..9386
                     /gene="rlmD"
                     /locus_tag="VC122_001336"
                     /EC_number="2.1.1.190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000902660.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (uracil(1939)-C(5))-methyltransferase
                     RlmD"
                     /protein_id="HEP5641258.1"
                     /translation="MLKKNDIIQVAISDLSHEGAGVAKHDGFVFFVDNALPEEVIDMR
                     VLKVNKNSGFGKVEAYHYLSPARNADVNLTYLRTGIADLGHLTYEDQLTFKKKQVQDS
                     LYKIAGISDVTVESTIGMTEPLAYRNKAQVPVRRVNGQLETGFFRKHSHDLIPISDYY
                     IQDKEIDRLINFTRDLLRRFDIKPYDETEQTGLLRNIVVRRGHYSGEMMLVLVTTRPK
                     VFRVDQVIEKIVEAFPAVVSIIQNINDKNTNAIFGKDFKTLYGKDTITDSMLGNNYAI
                     SAQSFYQVNTVMAEKLYQTAIAFSDLSKDDIVIDAYSGIGTIGLSFAKTVKAVYGVEV
                     IEAAVRDAQHNAALNGITNAYFVADTAEHAMATWAKDGIKPSVILVDPPRKGLTESFI
                     QASVAMGPQKITYVSCNPATMARDIKRYQELGYKLAKVQPVDLFPQTHHVETVALLSK
                     LGVDKHINIEIEMDELDLTSAESKATYAQIKEYVWNKFELKVSTLYIAQIKRKCGIEL
                     REHYNKSKKEKQIIPQCTPEKEEAIMDALRHFKMIE"
     gene            9542..>9844
                     /locus_tag="VC122_001337"
     CDS             9542..>9844
                     /locus_tag="VC122_001337"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013852041.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ClbS/DfsB family four-helix bundle protein"
                     /protein_id="HEP5641259.1"
                     /translation="MRSYESKEELKNEIKKTFEKYISEFDNIPEELKDKRLEEVDRTP
                     AENLAYQVGWTTLVLKWEEDEKKGIDVKTPSDKFKWNQLGELYQWFTDTYAHKSLYE"
BASE COUNT         3278 a         1627 c         2015 g         2924 t
ORIGIN      
        1 ctgctagtga cgcctgggaa aaagcagcaa ctccaaccgc tttagatgtt aagaaaacta
       61 aagatacaac acctgtagtt aaaaaagaag aaagacaaaa cgttaatacc cttcctacaa
      121 ctggtgaaga gtctaaccca ttctttacag ctgctgcgct tgcaataacg gtaagtacag
      181 gtgcgttagt tgtaagttca aagtgcaaag aaaattagtt gctatttgtt ctagcaaatg
      241 aaacaagaga atcgaaagat tctctttttc tgatatttaa aaatgctaca gtttataata
      301 gcttgaaaga ctaaatgttc tcaaactcat tagaacaagc tttaataaaa ctaacccagt
      361 tgtatcaaag gatagataaa gactaagact gttatagaat atttgcaaaa gatttgttat
      421 aatggaccca atacaggtat tgtgaaaggt ataatatgga tatttggaca aagcttgcag
      481 tgtttgcttt ttttgagact ccgaaagtaa tattacggcc ttttcgctat gaagatcatt
      541 gggattttta cagcatggtt aacgacacta aaaaccttta ttatgttttt ccagaacaaa
      601 aaactaaggc agcaagtgac tatcttttag tacatagttt tataaagttt cctttaggtc
      661 agtgggcaat agaagataaa gcaacccacc aagtaatagg ttctattaga attgagcatt
      721 atgatgctaa aacgcgttgt gctgatattg gctatttttt aaactatgcc ttttggggac
      781 aaggaattat gacagaagtc gtaataaaac ttgtttattt atcttttcac gaatttggtc
      841 tcaaaacgtt gcgtattata actcatttag aaaataaggc cagccagaaa gtagctaaaa
      901 aagcaggttt tcaactaaaa acctgtttta aaggaagtga tcgtaatact cataaaatct
      961 gcatttataa aatgtaccaa ctaactaatg ataggtgata atcattatga gcaaacatca
     1021 agatatttta gattatttag aaaaacttgc tattggtaaa aaagtgagtg ttagaagcat
     1081 ttcaaaccac ttaaaagtaa gcgatggaac agcctaccgt gctattaaag aggcagaaaa
     1141 tcgaggtatt gtcgaaacca aaccaagaag tggaactgtt cgtattgaaa aaaaaggacg
     1201 ggttcgtatt gaccgtctga cctattccga aattgctcgg atcagtgatt cggaagtgct
     1261 agcgggacat gctggtttag gacatgaatt cagtaggttt tcaattggtg ctatgactca
     1321 acaaaatatt cgtcgttact tggtcaaagg agggttatta atagttgggg atcgtgaaac
     1381 gattcaactc ttagctcttg aaaaccataa tgccattttg gtgacgggcg gatttccagt
     1441 atccaaacgt gttattgaaa tggcaaataa ccaacgaatc ccagttatgg taactcatta
     1501 tgataccttt actgtggcaa caatgattaa tcacgcgcta tctaatattc gtattaaaac
     1561 agatctcaaa acagttgaac aggtaatgat acctattaca gattatggtt acctttgcga
     1621 aaacagctct gttgaagaat tcaatacatt aataaaaaaa acacgacaag tcaggtttcc
     1681 tgtattagat tataaaagaa aagtgattgg tgtagttagt atgcgtgatg tggtggatca
     1741 gttgccaaca actaagctta ccaaggtcat gtctaaaaat ccaataacgg ctagaccaaa
     1801 tactagtctt gctaatatta gccagaaaat gatctttgaa gatttaaaca tgttacctgt
     1861 cactgacgaa gaaaacaatt tacttggtat gattactcgt agacaagcaa tggaaaattt
     1921 gccaaatcac caacccaata acccctatac ttatagtgag caaatacttt ctaaccttga
     1981 agaaacggtt gattattacc aagtagtagt tgagccaaca atgattgata gtgctggtaa
     2041 catgtcaaac ggtgttattt cagagttctt aaaggaaatt agtatcagag ccctcacgaa
     2101 aaagcaccaa aaaaatatca ttatcgaaca aatgatggta tactttttac atgctattca
     2161 aattgaggat gaattaaaaa tatatcctaa aatcattact gaaaatagac gaagcagcac
     2221 cattgatatt gaaatttttg tggatgatca agtgattgct aaagctatta ttacaacgaa
     2281 aataaattag aggagtaaca aaacaatgat aacattaaaa tcagcccgtg aaatagaagc
     2341 gatggataga gcaggagact tcttagcagg tatccatatt ggcttaaggg atattattaa
     2401 gcctggtgtg gatatgtggg aagtggaagc atatgttcgt cgccgttgta aagaagacaa
     2461 cgttcttcct ttgcaaatag gggttgatgg tcacatgatg gactatcctt atgcgacctg
     2521 ttgtggtcta aatgacgaag tagcacatgc ctttccccgt cattacattc tgaaagaagg
     2581 ggatttgctt aaagttgaca tggtactcag tgagcctttg gataaatcaa ttgttgatgt
     2641 agcagcttta gattttgata atgtgcctga gatgaagaaa tggacaagat cttatactgg
     2701 tggtttagct gactcttgtt gggcttatgc tgttggaaca ccgtctgacg aaataaagca
     2761 attaatggat gtcaccaaag aagccatgta ccgtggtatt gaaaaagcag taattggcaa
     2821 tcgtattggt gatattggcg cagctgttca agaatacgct gaaagcttcg gttatggtgt
     2881 cgtgcgtgat cttgttggtc acggtgttgg tcctacaatg catgaagagc caatggtacc
     2941 aaactatgga acagcaggtc gtggacttcg tctaaaagaa ggcatggtct tgactgttga
     3001 gccaatgatt aatacaggaa cttgggaaat tgataccgac atcaagacag gttgggctca
     3061 taaaacccta gatggtggtt tatcttgcca atacgaacac caatttgtga ttactaaaga
     3121 tggtcccgtt attttaacaa gtcaaggaga agaaagaact tactaaaatg gcagaaaaaa
     3181 aatggtttga taaggtactg tccaagtggc agtatgaacc cattcaagtc tttatgaggc
     3241 atttacaaag tgcagaaatg gatttatctg ccattgcggt tgcgtattac cttatcttaa
     3301 cggcctttcc attaattgtt attgcagcca atatttttcc ttacctcaat attgatattg
     3361 ctgatttgtt acgtttaatg aagcaaaatt tgcctaaaga tatttttaga ccggcttctg
     3421 caattgtaga aaatattttc tctaaaccgt ctggaagtgt tttgggagta gcgactttga
     3481 caggattatg gacaatgtct cgaagtttga catcgctgca aaaagctatt aataaagcct
     3541 atggcgcttc tcagcatcga gatttcttta tcggacactt ggttggtttg ttgaccagtt
     3601 taattatttt atttttatta gcctttgcct taatcttttc aacattttct aaagctgcta
     3661 ttcaagtgct ggataagcat taccatttga gtgataatat cacaacgatt ttcttactcc
     3721 tcattcaacc tattacagtt ttgattattt ttgttggctt gatgctacta tattttttat
     3781 tacccaatgt taagattaaa aaaattcgct acattttacc aggaacccta tttacatctt
     3841 ttgtgatgac atttttgagt aatctggttg gcaattacgt tgtgtataat gttgagcgga
     3901 tggtagatat taaaatgttt ggttcagtta tgatttttat catcatgcta tggtttattt
     3961 tcttagcaag aatcctgatt ttaggagcta tatttaatgc gacctatcaa gaaatgtcct
     4021 tggggaaatt agaaggtcgt agtggtgata tgatcgctat ccttaaaaaa acacttggga
     4081 atgattcaga tctatctcct tcacagtcta tcgaagatag tcacactgat tgatacaaaa
     4141 aagataataa gtcgtggagt gagaaactat tttcttgctt cattttttat gaggataaat
     4201 agaaaatcat tgatcttagc aaatagaaag gcagataaaa cggatgaaat tgcgaacaaa
     4261 tgcaggacca ttacagggaa cgattcaagt tccaggagac aagtccatta gtcaccgggc
     4321 agtcatatta ggagctgttg ctaaagggga aacacgagta aaaggactct taaaaggaga
     4381 agacgtgctt tccacgattc aagcctttag gaatttgggg gtccgtatcg aagaaaaaga
     4441 tgaccagctt gtgatagaag gtcaaggttt tcaaggattg accgctcctt gtcagacact
     4501 taatatggga aattctggaa cctctatgcg cctcattgct ggcttattgg caggtcaacc
     4561 ttttagtgtc aaaatgattg gcgatgaaag cttatccaaa cgtcctatgg atcggattgt
     4621 ttatccgtta aagcaaatgg gggttgagat atctggagaa acagataggc aatttcctcc
     4681 actacaattg caaggtaatc gtaatttaca accaatcaca tatactttgc ctatctcttc
     4741 tgctcaggtt aagtcagcta ttttgttagc agctttgcaa gcaaaaggaa caacacaggt
     4801 tgttgaaaaa gagattactc gaaaccatac agaagaaatg attcaacaat ttggtggacg
     4861 attgatagtt gatggtaaac gcattacttt ggtgggaccc cagcagctca ctgctcaaga
     4921 gataactgtc cccggagata tttctagtgc agctttttgg cttgtcgccg gcttaattat
     4981 tcctggttcg gagctccttt taaagaatgt tggagtcaat ccgacacgaa cagggattct
     5041 tgaagtggtt gaaaaaatgg gagcacagat tgtctatgaa gatatgaata agaaagagca
     5101 agtaactagt attcgagtgg tatattctca cttgaaagga actatcatat caggaggtct
     5161 tatccctcga ttgattgatg aacttcccat cattgcactt cttgcaaccc aagcacaagg
     5221 aacaacctgt attaaagatg ctcaagaatt acgagtcaaa gaaacagatc gtattcaagt
     5281 agtaactgat attcttaaca gtatgggagc taatatcaaa gcgacagcag atggcatgat
     5341 tatcaaaggc cctacggtac tatatggggc taacacaagt acctatggag atcatcgtat
     5401 tggtatgatg acagcaattg cagccttgct tgttaaacaa ggacaggtcc atttggataa
     5461 agaagaagcc atcatgacgt cttatccaac gtttttcaaa gatttagaaa ggctgtgtca
     5521 tgactaaagt gttattggga tttatgggag tggggaaaac aacggtttcg aaacacctaa
     5581 gtatgcactg taaagatatg gatgctatta ttgaagctaa aatcggtatg tctattgcag
     5641 ctttttttga acagcatgga gaaatagcat ttcgaacaat tgaaagtcaa gtgttaaaag
     5701 atctattgtt tgcaaatgat aatagtatca tcgtaacagg tggcggtgta gtggttttac
     5761 aagaaaatcg acaattactg agaaaaaatc accagcataa tattttattg gtagcttctt
     5821 ttgaaacttt gtaccagcgt ttaaaacatg ataaaaagtc tcaaagacct ctatttctta
     5881 agtactctaa ggaagcattt tatgaatttt atcaacaacg tatggtattt tatgagggac
     5941 tttcagattt agtaatcaga gttgaccatc gtacgcctga agaagttgcc aacatcattg
     6001 agggttatta gtaggtcatt gtcgtaatct gatatttatg gttttaaaca ggtatctgtc
     6061 taaagcactt cttatggcaa gttggcttta ttaaatatta ggaaataatc ttgattattc
     6121 tataatgatt aaaattttga gaggacaatc tggctaatat aggttataat ggtagtattc
     6181 gttaaaagtg caattgtaat actaagagat gataagatat gacaaaatac cccatgggtg
     6241 gccttagtca ccatgaagaa ttacgttatt tttatctcct aaggaatttg agttacctta
     6301 gtgagaatga aaaaaaagag tttgcttttt taaaatctaa attggaaatt ggtcgagctt
     6361 atgctccttc taagcagcat tataggaagt caaaacggca agaaccttac tttgaagatg
     6421 actactataa cgactactca ccaaatgatt tactagaaga tgatgacgtc aatcatgact
     6481 cttcttttgt tccatatggt ttaccgatat atcctaaaga agacagatat ttaaacaaaa
     6541 aaacaaaact tacagctcgt agacctatag atactccgca gcctattgat gaagacgatg
     6601 cttttttaac agagtctgtt gctcgttgtg ctcttcctag atctcaaaaa agaaagcata
     6661 agaaaaaagg atgtatgaaa tggtttttta acattcttgg cttattgcta atgactgtgc
     6721 ttatggggtt agggttgatg tttgcaaaag gggtttttga tatttctact aataaagcta
     6781 attacaaacc agctgtttcc caagcttttg atggtcaaga aactcaagat ggtacgaaca
     6841 ttctggtttt gggttctgac caacgagtta ctcaaggttc tacagatgct cgcacagata
     6901 ccattatggt tgtcaatgtc ggcaaccatg ctaaaaaaat aaaaatggtc tcttttatgc
     6961 gtgacacttt gattaacatt ccaggataca gttataatga taattcgtat gatcttaaat
     7021 taaatagtgc atttaatctt ggagagcaag aagatcacca cggagcagag tacgttcgac
     7081 gggccttaaa acataatttt gatatcgaca ttaagtatta tgtgatggta gattttgaaa
     7141 catttgcaga agctattgac accttgtttc caaatggtgt taagattgat gccaaatttg
     7201 cgacagtagg tggtgtagct gttgactccg tagaagttcc agatgacctg cgaatgaaaa
     7261 atggggttgt tcccaaccaa accatcgaag ttggagaaca acgcatggat ggtagaacct
     7321 tgctcaacta tgctcgtttc cgtaaagatg acgaaggaga tttcggacga accgtccgtc
     7381 agcaacaggt tatgtcagct gttatgtccc aaatcaaaga cccaactaaa ctctttacag
     7441 ggtctgcggc tattggtaaa atctatgcgc tgacttcgac caatgtttct tttccatttg
     7501 ttgttaaaaa tggtgtttct gttttaggaa gtgggaaaaa tggagttgag cacgtgacca
     7561 tcccagaaaa cggggattgg gttgacgaat acgatatgta tggaggacaa gccctttaca
     7621 tagattttga taagtatcag aaaacgttag ccaaattagg tctgcgctaa gatatggtat
     7681 aatggagagt aactaaagtt gctcttcttt tttgagcaat ggttagccct agaaaaggaa
     7741 aaagaataag aatgttgaaa aaaaatgata ttatccaagt tgctatttca gatttaagcc
     7801 atgagggagc aggtgtcgct aaacacgatg gttttgtttt ctttgttgat aatgccttgc
     7861 cagaagaagt gattgacatg cgtgtcttaa aggtcaataa aaacagcggt tttggcaaag
     7921 tagaagccta tcattacctg tcgccagcta gaaatgcaga tgtcaattta acttatttgc
     7981 gaacaggcat tgcagacctt ggacatctaa cttatgaaga ccaactgact ttcaaaaaga
     8041 aacaagttca ggacagcctc tataaaattg ctggaatcag tgacgtgaca gtcgaaagca
     8101 cgattgggat gacagaacct ttagcttacc gaaataaggc tcaggttcct gttagacgtg
     8161 ttaatggtca gctagaaaca ggctttttcc gcaagcattc tcatgacttg attcctattt
     8221 cggattatta tattcaggac aaagagattg atcgtctcat taattttacc cgtgatttat
     8281 tacgccgctt tgatattaaa ccgtatgacg aaacagaaca aacaggtctc ttgagaaata
     8341 ttgttgttcg ccgaggccat tactctggag aaatgatgct ggtcttagtg actacacgcc
     8401 caaaagtttt ccgtgttgac caagtcattg aaaaaatcgt tgaagctttt ccagcagtgg
     8461 tgtctattat tcaaaatatt aatgataaga acaccaatgc aatctttggt aaagatttca
     8521 aaacgcttta cggcaaggat accattactg atagcatgct tggaaacaac tatgccattt
     8581 ctgcccagtc tttctaccaa gtgaatacgg ttatggcaga aaaactctat caaacagcta
     8641 tagccttttc agacctttct aaagatgaca ttgtgattga cgcctactca ggtatcggaa
     8701 ccattggcct atcatttgct aaaacagtta aagctgttta tggtgttgaa gtgattgaag
     8761 cagctgttag agatgctcaa cacaatgccg ctcttaatgg catcaccaat gcctactttg
     8821 tcgcagatac cgcagaacat gccatggcaa catgggcgaa agatggcatt aaaccaagtg
     8881 ttattctggt agacccgcca cgaaaaggct taacagaaag tttcatccaa gccagcgtgg
     8941 caatgggacc acaaaaaatt acctacgttt cctgcaaccc agccaccatg gcgcgtgaca
     9001 tcaagcgtta tcaagagttg ggttacaaac tggccaaagt acagcccgta gatttgtttc
     9061 cccagacgca tcacgtggag acggtagcac ttttgtccaa acttggtgtc gataagcata
     9121 taaatattga aattgagatg gatgagcttg atttgacaag tgctgaaagt aaggcaacat
     9181 atgctcaaat caaagaatat gtttggaata aatttgaatt aaaagtttcg acattatata
     9241 ttgcacagat aaaaaggaaa tgtggaatag aattacgaga acattacaac aagtctaaaa
     9301 aggagaaaca aattattcca cagtgtacac ctgaaaaaga agaagccatc atggatgctt
     9361 tgagacattt caaaatgatt gaatagaaaa gaatgacagc atatcacttt ctgcatttat
     9421 tacattccta tttggtataa gaacaactat tattcttttc ttgcaaggta tcaattagaa
     9481 aatagggtca atataaagaa tggtaggata atacctatat ataaaccatg gaggtaggca
     9541 tatgaggtct tatgaaagta aggaagaatt aaagaatgag ataaagaaaa cttttgaaaa
     9601 atatatttca gagtttgaca atataccaga agaactaaaa gataaaagac tagaagaagt
     9661 tgataggaca ccagcagaaa atcttgccta tcaagtaggc tggacgactt tagtattaaa
     9721 atgggaagaa gatgagaaaa aaggtataga tgttaaaaca ccatcagata aattcaagtg
     9781 gaatcaactt ggagaattat atcaatggtt tacagatacc tatgcccata aatctctata
     9841 tgaa
//
LOCUS       DATXIG010000048         9599 bp    DNA     linear   BCT 28-DEC-2023
DEFINITION  TPA_asm: Streptococcus pyogenes strain MGAS11113
            SAMN08555799-rid19682583.denovo.048, whole genome shotgun sequence.
ACCESSION   DATXIG010000048 DATXIG010000000
VERSION     DATXIG010000048.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN08555799
            Sequence Read Archive: SRR6740495
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pyogenes
  ORGANISM  Streptococcus pyogenes
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 9599)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9599)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-OCT-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-OCT-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001918058.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001918058.2
            Genome Coverage       :: 368x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/12/2023 11:23:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 1,806
            CDSs (total)                      :: 1,769
            Genes (coding)                    :: 1,722
            CDSs (with protein)               :: 1,722
            Genes (RNA)                       :: 37
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1, 1 (5S, 23S)
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 47
            CDSs (without protein)            :: 47
            Pseudo Genes (ambiguous residues) :: 0 of 47
            Pseudo Genes (frameshifted)       :: 30 of 47
            Pseudo Genes (incomplete)         :: 20 of 47
            Pseudo Genes (internal stop)      :: 9 of 47
            Pseudo Genes (multiple problems)  :: 11 of 47
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9599
                     /organism="Streptococcus pyogenes"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN08555799-rid19682583.denovo.048"
                     /strain="MGAS11113"
                     /serotype="Emm28"
                     /isolation_source="normally sterile site"
                     /host="Homo sapiens"
                     /db_xref="taxon:1314"
                     /country="Finland"
                     /collection_date="2002"
                     /collected_by="National Public Health Laboratory"
     gene            <1..1158
                     /locus_tag="VC122_001338"
     CDS             <1..1158
                     /locus_tag="VC122_001338"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000619317.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEP5641260.1"
                     /translation="IFVSITSLFVNSILFNFLFLMVFTILHLYPLLVIVLYYRSFRQQ
                     ISVVRRSLILFSLLLLVILGSELYGEMLDVNQAFNNLGWYLFPLIMSVIYYFKTIHDK
                     LSFVTQRWLGDYKARIELLFLLLILCWIVLMKVLVKDFLLFFIVVDASTLFCLVVISC
                     IFYYLKDSKQNFDYENRRLNYFMKSEENMRVEFSNYLHDDVLQNIIAIKNLLSLENSN
                     ITHGFIVNELNDLVSGIRDEMDTYYPIVPANQTMKENIQSLFDDIVKSRKSNTLLYFN
                     CSDNMVVPSPYGDIVYRFIKELINNAIKYGDGKDIRLSLTIQSDIIIIEESNQVVEKV
                     HSISYGRGLKSFQETLAAFDGDLELQMDTKQFTIRILLPIDWKLCYEDFIN"
     gene            1139..1744
                     /locus_tag="VC122_001339"
     CDS             1139..1744
                     /locus_tag="VC122_001339"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015057302.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HEP5641261.1"
                     /translation="MKILLIDDHRLFAKSIQLLFQQYDEVDVIDTITSHFNDVTIDLS
                     KYDITLLDINLTNISKENGLEIAKELIQSTPHLKVVMLTGYVKSIYRERAKKVGAYGF
                     VDKNIDPKQLISILKKVDSGKKYFEQIESQDYVESLTDQEIAILNLSKKGFSIKEIEE
                     TLQISRRTVFNHLTHIYSKLLVNNKQEAIYKAEQLGYFMDF"
     gene            complement(2296..3876)
                     /locus_tag="VC122_001340"
     CDS             complement(2296..3876)
                     /locus_tag="VC122_001340"
                     /EC_number="2.1.1.72"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002982804.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I restriction-modification system subunit
                     M"
                     /protein_id="HEP5641262.1"
                     /translation="MAEKTTSLRQALWHSADQLRGQMDANDYKNYLLGLIFYKHLSDK
                     LLFAVCDNLEEHFNTFTDAQKIFENAYQDEGLKDDLISVVTGDLGYFIEPTLTFEKLI
                     QDVYHNTFQLESLAQGFRDIEQSGEDFENLFEDIDLYSKKLGSTPQKQNQTISNVMKT
                     LNEIDFEAVDGDTLGDAYEYLIGEFASESGKKAGEFYTPQAVSHLMTQIVFLGWEDQK
                     GMTLYDPAMGSGSLLLNAKKYSNQSDTVSYYGQEINTSTYNLARMNMMLHGVAIENQH
                     LSNADTLDADWPTDEPTNFDGVLMNPPYSLKWSATAGFLTDPRFSSYGVLAPKSKADF
                     AFLLHGFYHLKNTGTMAIVLPHGVLFRGAAEGKIRQKLLEQGAIDTIIGLPSNIFYNT
                     SIPTTIIILKKNRTNKDVFFIDASKEFDKGKNQNTMTDSHIKKILDAYKSRDNSDKFS
                     YLASFDEIIENDYNLNIPRYVDTFEEVPVKPLPELAKQLSDIDQEIAKTNAKLDQLMK
                     QLVGTTKEAQDELDTFRN"
     gene            complement(3889..5076)
                     /locus_tag="VC122_001341"
     CDS             complement(3889..5076)
                     /locus_tag="VC122_001341"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002982807.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="restriction endonuclease subunit S"
                     /protein_id="HEP5641263.1"
                     /translation="MTKSKRPQYRFDSFEGEWEEKKLGEISNIVRGASPRPIQDPKWF
                     DAKSDIGWLRISDVTEQEGRITYLQQRISELGQEKTRVLKDPHLLLSIAATVGKPVIN
                     YVKTGVHDGFLVFLDPKFNREFMFQWLDMFRPYWNKYGQPGSQVNLNSEIIRNQVINL
                     PSLPEQEAIGELFQTVDQLIQLQRQKLAILKEQKQTFLRKMFPAQGQKVPEIRLQGFD
                     GEWEEKELGDIVQITMGQSPSSQNYTTNPSDYILVQGNADIKNGYVFPRVWTTQITKQ
                     ADKGDIILSVRAPVGDVGKTNYHVIIGRGVAAIKGNEFIFQILKYLKEIGYWKRISTG
                     STFDSISSSDIKYAKIQIPSLSEQEAIGNFFQTLDQQIAQSEEKLTELKALKQTLLNR
                     LFV"
     gene            complement(5089..8067)
                     /locus_tag="VC122_001342"
     CDS             complement(5089..8067)
                     /locus_tag="VC122_001342"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011054992.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I restriction endonuclease subunit R"
                     /protein_id="HEP5641264.1"
                     /translation="MGNQTKTELELEKELIHLLETGESQWTYRKELKTEDALWDNFFK
                     ILAQNNTQYLNEEPLTASEKEQIKNQLNFVNYYEAAKWLAGENGIAKVQVQREDAKLG
                     TIRLEVVKADNVAGGTSVYEIANQVAFSGSRDRRGDVTLLINGLPMIQIELKSQNHQC
                     IEAFNQVKKYDKEGQFRGIFSTLQMFVVSNKTDTRYIAAAKENKLNPNFLTQWVDQNN
                     KPQKDLFAFAKEVLSIPRAHQMVMTYSVIDDDKKALILLRPYQIHAIEAVAEASRHRK
                     SGYIWHTTGSGKTLTSYKVARNILQIPAVEKSIFVIDRKDLDNQTASAFQSYAQNDIF
                     DVDETEDTRQLIKNLESSDRRVVATTIQKLNAMISQMESYDTPKFKKLKERLAHLNVV
                     FVVDECHRAVTPERQRYLTNTFRNSRWYGFTGTPIFVENKRAQLGDLAQTTEQQYGKC
                     LHQYTVKEAIHDKAVLGFQVEYKTTIPDMPEDSIPEEAYDHEEHMLAVLDSIINQSRK
                     KLGFNNGIGQTFEGLLTVKSIARAQAYYDLMKKVKAGETDLVISKKVKEKLPDFPKVA
                     ITYSITENDNASISRQDKMTKNLEDYNHLFGTNFTIDNLQGYNRDLNDRLARKKDKFK
                     DRHEQLDLVIVVDRLLTGFDAPCLSTIFIDRQPMKPQHIIQAFSRTNRIFESRKHYGQ
                     VVTFQTPLRFKEAVDTALSLYSNGGQSEVLAPSWEEEKARFFEKVTVLKNIVPDPDAF
                     PTIESAQTAFLKQYAKAFQAFDKLYASVQVYSDFNETLLSEVGLSDEVIDTYKGTYQN
                     VIAEIRKRREDDEAIPEINIDYELESVQMDDINYHYILTLIQAFVDQEQEALQERLND
                     NPMDQYIQDLAKSNPAMADSLAELWQDIQKEPKAYEGKSIAYELDNLIGDKIQRAIKH
                     FADQWKADPDKLAFVATNYHSANSTKQVGISTLKESLDYQAYKEKQGDSAMNKLKYKS
                     QFERELVQFIRDQIQPLKGH"
     gene            8348..9490
                     /locus_tag="VC122_001343"
     CDS             8348..9490
                     /locus_tag="VC122_001343"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011285157.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerate kinase"
                     /protein_id="HEP5641265.1"
                     /translation="MTINRVLKDDNLLQYTEKEFIEDMKILVAIDSFKGSVTSPELNT
                     SVAQALLSVDKQLVIETRAIADGGEGSLAALSQTVAGRWHQVKTIDLLRRPIKVAYYR
                     HAKQAFIESASIIGIDKITPNSVTYAQATSYGLGLAVKDAIQKGATQIEIMLGGTGTS
                     DGGKGFLESLNYDFMTGRSYLDTLASPVTLLGLTDVTNPYHGPQGFAAVFGPQKGGSL
                     SQIEETDQIASNFAKKVFYQKTIDLQTIPGSGAAGGLGGAIVLLGGTLTSGFSRIAEL
                     LNLDNSLQSCDLVITGEGCLDTQSQSGKVPVAIARMAKKYQVPTIALCGSVKIETGLA
                     AEDFLAVFSIQQQPISLEAAIDKTTTLSNIKILVANLMLLIAQFNK"
BASE COUNT         2896 a         1633 c         1815 g         3255 t
ORIGIN      
        1 atttttgttt ctataactag cctttttgtt aatagcattt tatttaattt tttattcctg
       61 atggttttta ctattttaca cctgtatccg ttactagtta tagtgctata ttataggtcc
      121 tttaggcaac aaatatcagt tgttagacgt tcattgatac tattttcttt acttttgctg
      181 gtaatacttg gtagtgaact ttatggagaa atgctagatg ttaatcaagc ctttaataat
      241 ttgggatggt atctgtttcc ccttataatg agtgtaatat actattttaa gaccatacat
      301 gataaattga gttttgttac ccaaagatgg ttaggtgatt acaaagctcg tatagaacta
      361 ttatttctac ttcttatcct ttgttggatt gtactcatga aggtattagt taaagatttt
      421 ttgctatttt ttatagttgt tgacgcaagc acattgtttt gtctagtggt aatttcttgc
      481 atcttttatt atttaaaaga ctccaaacaa aattttgact atgaaaatag aagactgaac
      541 tattttatga aatcagagga aaacatgaga gtggagttct caaattattt gcatgatgat
      601 gttttacaaa atatcatagc gataaaaaac ttgctttctc tagaaaatag taatataaca
      661 catggtttta ttgttaatga gttgaatgat ttagtttctg gtatcagaga tgaaatggat
      721 acttactacc caatagttcc cgctaatcaa acaatgaaag aaaacataca gtctcttttt
      781 gatgatattg ttaaaagtag aaaaagtaat accctattgt attttaactg ttcagataat
      841 atggtagtgc catccccata tggagatatt gtttataggt ttataaaaga gctaataaac
      901 aatgctataa aatatggaga tggaaaagac attcgtttat ctttaacgat tcaatctgat
      961 attattatca ttgaggagag caatcaagta gtggaaaaag tacactctat aagttatggc
     1021 agaggattga aatcatttca agaaacacta gcagcttttg atggagatct agaactacaa
     1081 atggatacta aacaatttac tatcagaata ttacttccca tagattggaa attgtgttat
     1141 gaagatttta ttaattgatg accacagact atttgcaaag agtatccaac tgttattcca
     1201 acagtatgat gaagtagatg tcatagatac gattacttct cattttaacg atgtgacgat
     1261 tgatttgtct aagtacgata ttacattgct tgatattaat ttaacaaata tttctaaaga
     1321 aaatggattg gagatagcaa aagaactcat acagtctact cctcatttga aagttgttat
     1381 gttgacagga tatgttaaat caatctatag agagagggca aaaaaagtag gagcatacgg
     1441 ttttgtagat aaaaatatag accctaaaca actcatttct atcttaaaaa aagtggattc
     1501 tggaaaaaag tattttgaac aaatcgaatc tcaggattat gttgaatctt taactgacca
     1561 ggaaatagct atcttaaatt tgagtaagaa aggattctct ataaaagaaa tagaggaaac
     1621 gttacaaatt agtaggagaa cagtatttaa tcatttgacc cacatctact caaagctttt
     1681 ggtaaataat aagcaagagg ctatctacaa agccgaacaa ttagggtatt ttatggattt
     1741 ctgagttgca gataatcaaa cgacataagt tttacttgtg tcgttgctag ggatttcctg
     1801 ttaaataccc agatttaaac tcattgtttt tgagttcttt ttgattgaaa ttgattgaga
     1861 taacgctttt ataaaaagta gggcaatgcc tgatatgaaa agctttttaa atttagtcaa
     1921 acaaaagttg tttaaaccag gtctaaaaaa actcgtaaag cttcacaact cccagaacgt
     1981 taatatatgc ttatatatca acgattggaa ctactttatg gttcgcacca tggtttttgt
     2041 ggaaggatca aaagttgtcc tgaaaatttc tcttaaccgt gtttaagctt tctaaacgtt
     2101 tgagaaatca ttgtaacttc aaataactat tttaaataaa acaaagaaac ggacaaagtt
     2161 agatgagatg attctatttt gatttatagg tgaagaagga gaatcttgtt tgataagtac
     2221 tcaataaagg ttcactaccc aattttaaat aaaacgccac agtagtaatc atctactgtg
     2281 gtgttttttg gtcttttagt ttctaaaagt atccaattcg tcttgtgctt ctttggttgt
     2341 tccgactagt tgtttcatga gttgatctag tttggcattt gttttggcaa tttcttgatc
     2401 aatgtcactg agctgtttag ctaattctgg cagtggtttg acggggactt cctcaaaggt
     2461 atcgacatag cgtgggatat ttaagttata gtcattttca atgatttcat caaatgaggc
     2521 taaatatgaa aacttatcag aattatcacg actcttataa gcatcaagga tctttttgat
     2581 atggctatct gtcattgtgt tttgattttt gcctttatca aactcttttg aagcatcgat
     2641 aaagaagaca tctttgttgg ttctgttctt ctttaatatg ataatagtcg tagggatgct
     2701 tgtattataa aagatatttg atgggagtcc gataatggta tcgatagccc cttgttcgag
     2761 tagtttttgg cgaatcttgc cttctgcagc accacggaaa aggacaccgt gtggtaagac
     2821 aatcgccata gttcctgtat tttttaaatg atagaaacca tgaagtaaaa aggcaaagtc
     2881 agcttttgat tttggtgcta atacaccata acttgaaaac cgtggatctg ttaagaaacc
     2941 agcagtcgct gaccatttca aagaataagg tgggttcatt aacactccat caaagtttgt
     3001 tggttcatct gttggccagt ctgcatccaa ggtatcagcg ttacttaagt gttggttttc
     3061 gatggcaacg ccatgaagca tcatgttcat acgagccaag ttataagtag aagtattgat
     3121 ttcttgacca tagtaagaga cggtatcgga ttggttacta tatttcttag catttaaaag
     3181 gagtgatcca gatcccatag cagggtcata aagggtcatt cctttttgat cttcccaacc
     3241 taaaaagaca atctgagtca tcaagtgaga gacagcttgt ggggtataga actcacctgc
     3301 tttttttcct gactcactgg caaattcacc aatcaagtat tcataagcat cacctaaagt
     3361 atccccatct actgcctcaa agtcaatttc atttaatgtt ttcatgacat tagaaatggt
     3421 ttgattttgt ttttgaggag tagagcctaa ttttttagag tagaggtcaa tatcttcaaa
     3481 taagttttca aagtcttcac cactttgttc aatatcgcga aaaccttgag ctaatgattc
     3541 taattggaaa gtattgtgat aaacatcttg gatgagtttt tcaaaggtca gtgtaggttc
     3601 gataaagtaa cctaaatcac cagtaacaac agaaatgagg tcatctttta ggccttcatc
     3661 ttgataggca ttttcaaaga ttttttgagc atctgtaaag gtattaaagt gttcttctaa
     3721 attatcacaa acagcaaaga gtaatttatc agataaatgt ttgtaaaaga tgagtccaag
     3781 aagatagttt ttatagtcat tggcatccat ttggccacgt aattgatcgg ccgaatgcca
     3841 tagtgcttgc cgtagtgaag ttgttttctc tgccatgtgc gtcctttctt atacgaataa
     3901 acgattgagt aatgtttgtt tgagtgcttt gagttctgtt agtttttctt cagattgagc
     3961 aatttgttgg tctaaagttt ggaagaaatt accaatggct tcttgttctg agagggaagg
     4021 gatttgaatt ttagcatatt tgatatcact agagctaatg ctatcaaaag ttgaacctgt
     4081 actaattctt ttccaataac caatttcttt caagtatttt aatatttgaa agataaattc
     4141 attacctttt atagcagcaa ctccacgccc aataataaca tgataatttg tctttccaac
     4201 atcaccaact ggagccctaa cactaagaat aatatcacct ttgtcagctt gttttgtaat
     4261 ttgtgttgtc cagacacgcg gaaaaacata tccatttttt atatcggcat ttccttgtac
     4321 tagaatataa tcagaagggt ttgtagtata attttgactt gatggagatt ggcccattgt
     4381 aatttgaaca atatcaccta gttccttctc ttcccactca ccatcaaaac cttgtaaacg
     4441 gatttcaggg actttttgtc cttgagctgg gaacatcttt ctgaggaagg tttgcttttg
     4501 ttctttaagg attgctagct tttgcctctg taactgaatt aactgatcaa ccgtttggaa
     4561 gagttcgcca atggcttctt gttcagggag ggaggggaga ttgataactt gatttcgaat
     4621 tatttcagaa ttcagattca cttggcttcc tggttgacca tatttattcc aatatggtct
     4681 gaacatatct aaccactgaa acataaattc acggttgaat ttaggatcta aaaacacaag
     4741 aaatccatcg tgtactccag ttttaacata attgataact ggtttaccta ctgtagctgc
     4801 aatacttaat agtaaatgtg gatcctttaa tactctagtc ttttcttgac caagctcaga
     4861 tattctttgt tgtaaataag ttatacgacc ctcttgttca gtaacatcag aaattcttaa
     4921 ccaacctata tcacttttgg catcaaacca ttttggatct tgtatcggtc taggactggc
     4981 accacgaact atattagaaa tttctcccag ctttttctct tcccactccc cttcaaaact
     5041 gtcaaagcga tattgaggcc gtttagattt tgtcatctct ttcctttcct agtgcccttt
     5101 taagggttgg atttgatctc taatgaattg aactaactcc cgttcaaatt ggcttttgta
     5161 tttgagttta ttcatggccg agtcaccttg tttttcttta taggcttggt agtctaggga
     5221 ttcttttaag gttgatatgc ccacttgttt ggtagagtta gcactatgat aattagtcgc
     5281 cacaaaggct aatttgtcag gatctgcctt ccactggtcc gcaaagtgtt tgatggccct
     5341 ttggatctta tcaccaatga ggttatccaa ctcataagca atgcttttcc cttcataggc
     5401 tttgggttct ttttggatat cttgccataa ctcagctagt gagtcagcca tagcagggtt
     5461 tgattttgcc aaatcttgga tgtattgatc catgggattg tcattgagcc tttcttgaag
     5521 agcttcttgt tcttgatcga caaaggcttg aattaaggtc aagatatagt gatagttgat
     5581 atcatccatc tgaacacttt ctagctcata gtcgatattg atctctggga tggcttcgtc
     5641 atcttctctt cgctttcgta tttctgcaat gacattttgg taagtgcctt tataagtatc
     5701 aatcacttca tctgataacc caacctcact caatagtgtt tcattaaagt cactatagac
     5761 ctgtacagat gcatagagct tatcaaacgc ttgaaaggct ttggcgtatt gtttgagaaa
     5821 agctgtctgt gcgctctcaa tagttgggaa agcgtctgga tcaggaacta tattttttaa
     5881 tactgttact ttttcaaaga atcttgcttt ttcttcttcc caagaaggcg ctaaaacttc
     5941 actttgacca ccatttgagt ataggctaag agccgtatca acagcttctt taaaacgaag
     6001 tggtgtttgg aaagtcacaa cctgaccgta atgttttcta ctttcaaaga tacggttggt
     6061 tcgtgagaaa gcttgaataa tgtgctgagg tttcataggc tggcgatcga tgaagatggt
     6121 tgataaacaa ggcgcatcaa agcctgttaa gagacggtca accacaatca cgaggtctaa
     6181 ttgttcatga cggtctttga atttatcttt tttacgggct aagcgatcat ttaaatctct
     6241 gttatagcct tgtaagttat cgatggtaaa gttagtgcca aagaggtgat tatagtcttc
     6301 tagatttttg gtcattttat cttgtcggct gatagacgca ttgtcatttt cagtgataga
     6361 ataagtaata gctacctttg gaaagtctgg tagtttttct ttgaccttct tagaaatgac
     6421 taaatccgtt tcaccagcct taactttctt catcagatca taataggctt gggcacgagc
     6481 aatactctta acggttagta aaccttcgaa tgtctgacca attccattat taaaaccaag
     6541 tttcttgcgt gattgattaa tgatactatc taagacagct aacatatgtt cttcatgatc
     6601 ataagcttct tccggaatac tgtcttcagg catatcagga atggttgtct tatactcaac
     6661 ttgaaagcct agaaccgctt tatcatgaat ggcttcttta acggtgtatt gatggagaca
     6721 tttgccgtat tgctgttccg ttgtttgggc taaatcacct aattgagcac gcttattttc
     6781 cacaaagata ggagtgccgg taaagccata ccagcgactg ttacgaaaag tattcgtgag
     6841 ataacgttga cgttccggtg tgacagcacg gtggcattcg tccacaacaa agacaacatt
     6901 taagtgtgca agacgctctt ttagtttctt gaattttgga gtgtcataac tttccatctg
     6961 actgatcatg gcattgagtt tttgaatggt ggtggccaca acacgacgat cactggattc
     7021 taaatttttg atgagttgtc tcgtatcttc tgtctcatcg acatcaaaga tatcattttg
     7081 cgcataggat tgaaaagcag aagcggtctg attatctaaa tcctttcggt caatgacaaa
     7141 gatggatttt tcaacggccg gtatttgaag gatattgcga gccactttat aggaagttaa
     7201 ggtttttcca gaacctgttg tgtgccagat atagccagac ttgcggtgac gactcgcttc
     7261 agcgacggct tcgattgcat gaatctgata aggacgaagg agaattagtg cttttttatc
     7321 atcatcaatg acagagtaag tcatgaccat ttggtgagca cgagggatag ataggacttc
     7381 tttggcaaag gcaaagagat ctttttgtgg tttgttgttt tgatctaccc attgggtcaa
     7441 gaagttaggg tttaatttat tttctttagc tgcagcgata taccttgtat ctgttttatt
     7501 tgagaccaca aacatttgta aggttgaaaa gatacctcta aactggcctt ctttatcata
     7561 ctttttgact tggttaaaag cttcaatgca ttgatgattc tgagatttta attcaatttg
     7621 gatcataggt aagccattga tgagaagtgt cacatcacct cgtctatcac gagaaccaga
     7681 gaaagcaact tgattggcaa tctcataaac agaagttcca ccagccacat tgtctgcttt
     7741 aacgacttct aaacgaatcg ttcctagttt tgcatcttcg cgttgcactt gtactttagc
     7801 aatgccgttt tcaccagcta accatttagc cgcttcatag taattgacaa agttaagttg
     7861 attcttaatc tgctcttttt cactagctgt taaaggctcc tcattcagat attgcgtgtt
     7921 attttgcgct aagatcttaa agaaattatc ccataaagca tcttcagttt ttaattcttt
     7981 acgatatgtc cattgacttt cacctgtttc taagagatgg atcaactctt tttcaagttc
     8041 aagttccgtt ttagtttgat ttcccatttt taatccccat tcgacatgat atagtaatat
     8101 tatatcattg gcggatcctt tttagaatgg caaagctatt gaaaccttcc aaacagtctt
     8161 tttttgatta gttgaatgct taagatccta gcaactaaac aaagctatga ctatgtaaat
     8221 gcaatcgaga aagttcttgt atttttgagt ttgcttgaca ataatttaag gcgaagaaag
     8281 taataagaca acactagtaa aaaggtcgag ttctcaaaat agataaaaaa cttaagacag
     8341 atctagcttg actattaata gggttttaaa ggacgataat ctgctacaat atacagaaaa
     8401 ggaatttatt gaagatatga aaattttggt agccatagat tcttttaagg gatcggtaac
     8461 gtctccagaa cttaacacta gtgtagctca agcccttctt tctgtcgata agcagttagt
     8521 tattgaaaca agagctatcg ctgatggagg agaaggaagt ttagcagccc tctcacaaac
     8581 cgtggcgggg aggtggcatc aagtaaaaac cattgatcta ttaagacgcc cgattaaagt
     8641 cgcttattat cgtcacgcaa agcaagcttt tattgaatct gcgagtatta ttggtattga
     8701 taagatcacc cccaattcag taacctatgc acaagccact tcctatggtt tgggtttggc
     8761 agttaaagat gccattcaaa aaggagctac tcaaatcgaa atcatgctag gaggaacagg
     8821 cacttctgat ggtggcaaag ggtttttgga aagtttaaac tatgatttca tgacaggaag
     8881 atcttatctt gatactttag catcacctgt tacgctatta gggctgactg atgtgaccaa
     8941 tccatatcat ggccctcaag gatttgctgc ggtctttggc cctcaaaaag gtgggagtct
     9001 tagtcaaatt gaagaaactg atcaaattgc aagtaacttt gctaaaaaag ttttttacca
     9061 aaaaaccatt gatttacaga caataccagg aagtggagca gctggaggat taggaggtgc
     9121 cattgtccta ttaggaggca ccttaactag tggttttagc aggattgcag agctactaaa
     9181 tctggataat tcccttcagt cctgtgattt agttattact ggagaaggat gcctggatac
     9241 acaatcacaa tctggcaagg tacctgttgc gattgcaaga atggctaaaa aatatcaagt
     9301 tccaacgatt gctctatgtg gttctgtaaa gattgagaca ggactagcag cagaagactt
     9361 tttggctgtt ttttcaatac aacagcagcc aatttcctta gaagcagcga tagataaaac
     9421 aacaacactt tccaatatca aaatattagt agctaacctt atgctattga tagcccagtt
     9481 taataagtaa actcatctat tttcgattgt gtagcattca tttggcggtt aaaagtaacc
     9541 tatgaagaag gagacgtacg agctaactca ttagcatagt atgaaaaaca gaaacattt
//
LOCUS       DATXIG010000049         9452 bp    DNA     linear   BCT 28-DEC-2023
DEFINITION  TPA_asm: Streptococcus pyogenes strain MGAS11113
            SAMN08555799-rid19682583.denovo.049, whole genome shotgun sequence.
ACCESSION   DATXIG010000049 DATXIG010000000
VERSION     DATXIG010000049.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN08555799
            Sequence Read Archive: SRR6740495
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pyogenes
  ORGANISM  Streptococcus pyogenes
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 9452)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9452)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-OCT-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-OCT-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001918058.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001918058.2
            Genome Coverage       :: 368x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/12/2023 11:23:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 1,806
            CDSs (total)                      :: 1,769
            Genes (coding)                    :: 1,722
            CDSs (with protein)               :: 1,722
            Genes (RNA)                       :: 37
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1, 1 (5S, 23S)
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 47
            CDSs (without protein)            :: 47
            Pseudo Genes (ambiguous residues) :: 0 of 47
            Pseudo Genes (frameshifted)       :: 30 of 47
            Pseudo Genes (incomplete)         :: 20 of 47
            Pseudo Genes (internal stop)      :: 9 of 47
            Pseudo Genes (multiple problems)  :: 11 of 47
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9452
                     /organism="Streptococcus pyogenes"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN08555799-rid19682583.denovo.049"
                     /strain="MGAS11113"
                     /serotype="Emm28"
                     /isolation_source="normally sterile site"
                     /host="Homo sapiens"
                     /db_xref="taxon:1314"
                     /country="Finland"
                     /collection_date="2002"
                     /collected_by="National Public Health Laboratory"
     gene            88..1008
                     /gene="lmb"
                     /locus_tag="VC122_001344"
     CDS             88..1008
                     /gene="lmb"
                     /locus_tag="VC122_001344"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003056036.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal ABC transporter substrate-binding
                     lipoprotein/laminin-binding adhesin Lmb"
                     /protein_id="HEP5641266.1"
                     /translation="MKKGFFLMAMVVSLVMIAGCDKSANPKQPTQGMSVVTSFYPMYA
                     MTKEVSGDLNDVRMIQSGAGIHSFEPSVNDVAAIYDADLFVYHSHTLEAWARDLDPNL
                     KKSKVDVFEASKPLTLDRVKGLEDMEVTQGIDPATLYDPHTWTDPVLAGEEAVNIAKE
                     LGRLDPKHKDSYTKKAKAFKKEAEQLTEEYTQKFKKVRSKTFVTQHTAFSYLAKRFGL
                     KQLGISGISPEQEPSPRQLKEIQDFVKEYNVKTIFAEDNVNPKIAHAIAKSTGAKVKT
                     LSPLEAAPSGNKTYLENLRANLEVLYQQLK"
     gene            1021..3498
                     /locus_tag="VC122_001345"
     CDS             1021..3498
                     /locus_tag="VC122_001345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009880581.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pneumococcal-type histidine triad protein"
                     /protein_id="HEP5641267.1"
                     /translation="MKKTYGYIGSVAAILLATHIGSYQLGKHHMGSATKDNQIAYIDD
                     SKGKAKAPKTNKTMDQISAEEGISAEQIVVKITDQGYVTSHGDHYHFYNGKVPYDAII
                     SEELLMTDPNYHFKQSDVINEILDGYVIKVNGNYYVYLKPGSKRKNIRTKQQIAEQVA
                     KGTKEAKEKGLAQVAHLSKEEVAAVNEAKRQGRYTTDDGYIFSPTDIIDDLGDAYLVP
                     HGNHYHYIPKKDLSPSELAAAQAYWSQKQGRGARPSDYRPTPAPAPGRRKAPIPDVTP
                     NPGQGHQPDNGGYHPAPPRPNDASQNKHQRDEFKGKTFKELLDQLHRLDLKYRHVEED
                     GLIFEPTQVIKSNAFGYVVPHGDHYHIIPRSQLSPLEMELADRYLAGQTEDNDSGSDH
                     SKPSDKEVTHTFLGHRIKAYGKGLDGKPYDTSDAYVFSKESIHSVDKSGVTAKHGDHF
                     HYIGFGELEQYELDEVANWVKAKGQADELAAALDQEQGKEKPLFDTKKVSRKVTKDGK
                     VGYMMPKDGKDYFYARDQLDLTQIAFAEQELMLKDKKHYRYDIVDTGIEPRLAVDVSS
                     LPMHAGNATYDTGSSFVIPHIDHIHVVPYSWLTRDQIATIKYVMQHPEVRPDVWSKPG
                     HEESGSVIPNVTPLDKRAGMPNWQIIHSAEEVQKALAEGRFATPDGYIFDPRDVLAKE
                     TFVWKDGSFSIPRADGSSLRTINKSDLSQAEWQQAQELLAKKNAGDATDTDKPKEKQQ
                     ADKSNENQQPSEASKEEEKESDDFIDSLPDYGLDRATLEDHINQLAQKANIDPKYLIF
                     QPEGVQFYNKNGELVTYDIKTLQQINP"
     gene            3667..3867
                     /locus_tag="VC122_001346"
     CDS             3667..3867
                     /locus_tag="VC122_001346"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000076713.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CsbD family protein"
                     /protein_id="HEP5641268.1"
                     /translation="MSEEKLKSKIEQASGGLKEGAGKLTGDKELEAKGFVEKTIAKGK
                     ELADDAKEAVEGAVDAVKEKLK"
     gene            complement(3949..4575)
                     /locus_tag="VC122_001347"
     CDS             complement(3949..4575)
                     /locus_tag="VC122_001347"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002982508.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEP5641269.1"
                     /translation="MTLEAKKLGFYHKKDQWLFKEIDLEVAPGQILGIFGQSGCGKTS
                     LSRVLAGFLQPKSGEVLVDGSHLPNKAFRPVQLIQQHPEQTMNPLWPMKKSLEEAYYP
                     SQDLRDAFGIQEKWLKRRPSELSGGELQRFSIVRSLHPETKYLIADEMTTMLDSITQA
                     SVWKSLLEIVKDRNLGLIIISHEFDMLEKLCDACYMIEENRTQLFKHT"
     gene            complement(4559..5362)
                     /locus_tag="VC122_001348"
     CDS             complement(4559..5362)
                     /locus_tag="VC122_001348"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000140979.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEP5641270.1"
                     /translation="MTETLLSIKDLSITFTQYGRFLKPFQSTPIQALNLEVKKGELLA
                     IIGASGSGKSLLAHAIMDILPKNAAVTGDMIYRGQSLTSKRIKQLRGKEMTLIPQSVN
                     YLDPSMKVKHQVRLGISENAKATQEGLFQQFGLKESDGNLYPFQLSGGMLRRVLFTTC
                     ISDTVSLIIADEPTPGLHPDALQMVLDQLRSFADKGISVIFITHDIVAASQIADRITI
                     FKEGKAIETAPASFFSGGGEQLQTEFARRLWRTLPQQDFLKGVTHDLRG"
     gene            complement(5374..6195)
                     /locus_tag="VC122_001349"
     CDS             complement(5374..6195)
                     /locus_tag="VC122_001349"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015631767.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEP5641271.1"
                     /translation="MILKRRTMVLWQLGIAISLILSILALNLYFYRTPLETNASLRNL
                     APSLNHLFGTDGLGRDMFVRTIKGLYFSLQVGLLGALMGVSLATVFGVLAGLGNSLID
                     KIIAWLVDLFIGMPHLIFMILISFVVGKGAQGVIIATAVTHWPSLARLIRNEVYDLKN
                     KAFVQLSKSMGKTPYYIVRHHILPLIASQIFIGFILLFPHVILHEASMTFLGFGLSAE
                     QPSVGIILSEAAKHISLGNWWLVVFPGLYLILVVNAFDTIGESLKKLFYPQTDHF"
     gene            complement(6192..7169)
                     /locus_tag="VC122_001350"
     CDS             complement(6192..7169)
                     /locus_tag="VC122_001350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000680642.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEP5641272.1"
                     /translation="MKRTTIIIIWKIIRCVTLIFGVSVLTFVLLKQSPVDPVMASVNY
                     DTSLTPAQYKAIAHHYGLDKPALVQYFIWLKNVIQGHLGTSLVYRQPVSDIIRSRAGA
                     SFILMGLSWILSGLIGFILGTLSAFHQGKLLDRVVRWFSYLQISVPTFWIGLIFLLIF
                     SVQLGWFPIGISSPIGTLSQDITLADRVKHLMLPVFTLSILGIANVTLHTRTKMMSVL
                     SSEYVLFARARGETQWQIFKHHCLRNAIVPAITLHFSYFGELFGGSVLAEQVFSYPGL
                     GSTLTEAGLKSDTPLLLAIVMIGTLFVFAGNLIADILNSIINPQLRRKV"
     gene            complement(7282..8910)
                     /locus_tag="VC122_001351"
     CDS             complement(7282..8910)
                     /locus_tag="VC122_001351"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017771899.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HEP5641273.1"
                     /translation="MSKYLKYFSIITLFLTGLILVACQQQKPQTKERQRKQRPKDELV
                     VSMGAKLPHEFDPKDRYGVHNEGNITHSTLLKRSPELDIKGELAKTYHLSEDGLTWSF
                     DLHDDFKFSNGEPVTADDVKFTYDMLKADGKAWDLTFIKNVEVVGKNQINIHLTEAHS
                     TFTAQLTEIPIVPKKHYNDKYKSNPIGSGPYMVKEYKAGEQAIFVRNPYWHGKKPYFK
                     KWTWVLLDENTALAALESGDVDMIYATPELADKKVKGTRLLDIPSNDVRGLSLPYVKK
                     GVITDSPDGYPVGNDVTSDPAIRKALTIGLNRQKVLDTVLNGYGKPAYSIIDKTPFWN
                     PKTAIKDNKVAKAKQLLTKAGWKEQADGSRKKGDLDAAFDLYYPTNDQLRANLAVEVA
                     EQAKALGITIKLKASNWDEMATKSHDSALLYAGGRHHAQQFYESHHPSLAGKGWTNIT
                     FYNNPTVTKYLDKAMTSSDLDKANEYWKLAQWDGKTGASTLGDLPNVWLVSLNHTYIG
                     DKRINIGKQGVHSHGHDWSLLTNIAEWTWDESTK"
BASE COUNT         3111 a         1822 c         1872 g         2647 t
ORIGIN      
        1 aagatggtag ttgccattga caaagcattg ataaaggagt aaaattaact ggttaataac
       61 tggttaaatt ataattgagg aggtactatg aaaaaaggtt tttttctcat ggctatggtc
      121 gtgagtttag taatgatagc agggtgtgat aagtcagcaa accccaaaca gcctacgcaa
      181 ggcatgtcag ttgtaaccag cttttaccca atgtatgcga tgacaaaaga agtatctgga
      241 gacctcaatg atgtgaggat gatccaatca ggtgcaggca ttcattcctt tgaaccgtct
      301 gtaaatgatg tggcagctat ttatgacgcg gatttgtttg tttaccattc acatacctta
      361 gaagcttggg caagggatct agaccctaat ttaaaaaaat caaaggttga tgtgtttgaa
      421 gcgtcaaaac ctttgacact agatagagtc aaagggctag aagatatgga agtcacacaa
      481 ggcattgacc ctgcgacact ttatgaccca catacctgga cggatcccgt tttagctggt
      541 gaggaagctg ttaatatcgc taaagagcta ggacgtttgg atcctaaaca caaagacagt
      601 tacactaaaa aggctaaggc tttcaaaaaa gaagcagagc aactaactga agaatacact
      661 caaaaattta aaaaggtgcg ctcaaaaaca ttcgtgacgc agcacacggc attttcttat
      721 ctggctaaac gattcggctt gaaacaactt ggtatctcgg gtatttctcc agagcaagag
      781 ccctctcctc gccaattgaa agaaattcaa gactttgtca aagaatacaa cgtcaagact
      841 atttttgcag aagacaatgt caatcccaaa attgctcatg ctattgcgaa atcaacagga
      901 gctaaagtaa agacattaag tccacttgaa gctgctccaa gcggaaacaa gacatatcta
      961 gaaaatctta gagcaaattt ggaagtgctc tatcaacagt tgaagtaaag gagattatta
     1021 gtgaagaaaa catatggtta tatcggctca gttgctgcca ttttactagc tactcatatt
     1081 ggaagttacc aacttggtaa gcatcatatg ggttcagcaa caaaggacaa tcaaattgcc
     1141 tatattgatg atagcaaagg taaggcaaaa gcccctaaaa caaacaaaac gatggatcaa
     1201 atcagtgctg aagaaggcat ctctgctgaa cagatcgtgg tcaaaattac tgaccaaggc
     1261 tatgtgacct cacacggtga ccattatcat ttttacaatg ggaaagttcc ttatgatgcg
     1321 attattagtg aagagttgtt gatgacggat cctaattacc attttaaaca atcagacgtt
     1381 atcaatgaaa tcttagacgg ttacgttatt aaagtcaatg gcaactatta tgtttacctc
     1441 aagccaggta gtaagcgcaa aaacattcga accaaacaac aaattgctga gcaagtagcc
     1501 aaaggaacta aagaagctaa agaaaaaggt ttagctcaag tggcccatct cagtaaagaa
     1561 gaagttgcgg cagtcaatga agcaaaaaga caaggacgct atactacaga cgatggctat
     1621 atttttagtc cgacagatat cattgatgat ttaggagatg cttatttagt acctcatggt
     1681 aatcactatc attatattcc taaaaaggat ttgtctccaa gtgagctagc tgctgcacaa
     1741 gcctactgga gtcaaaaaca aggtcgaggt gctagaccgt ctgattaccg cccgacacca
     1801 gccccagccc caggtcgtag gaaagcccca attcctgatg tgacgcctaa ccctggacaa
     1861 ggtcatcagc cagataacgg tggctatcat ccagcgcctc ctaggccaaa tgatgcgtca
     1921 caaaacaaac accaaagaga tgagtttaaa ggaaaaacct ttaaggaact tttagatcaa
     1981 ctacaccgtc ttgatttgaa ataccgtcat gtggaagaag atgggttgat ttttgaaccg
     2041 actcaagtga tcaaatcaaa cgcttttggg tatgtggtgc ctcatggaga tcattatcat
     2101 attatcccaa gaagtcagtt atcacctctt gaaatggaat tagcagatcg gtacttagcc
     2161 ggccaaactg aggacaatga ctcaggttca gatcactcaa aaccatcaga taaagaagtg
     2221 acacatacct ttcttggtca tcgcatcaaa gcttacggaa aaggcttaga tggtaaacca
     2281 tatgatacga gtgatgctta tgtttttagt aaagaatcca ttcattcagt ggataaatca
     2341 ggagttacag ctaaacacgg agatcatttc cactatatag gatttggaga acttgaacaa
     2401 tatgagttgg atgaggtcgc taactgggtg aaagcaaaag gtcaagctga tgagcttgct
     2461 gctgctttgg atcaggaaca aggcaaagaa aaaccactct ttgacactaa aaaagtgagt
     2521 cgcaaagtaa caaaagatgg taaagtgggc tatatgatgc caaaagatgg caaggactat
     2581 ttctatgctc gtgatcaact tgatttgact cagattgcct ttgccgaaca agaactaatg
     2641 cttaaagata agaagcatta ccgttatgac attgttgaca caggtattga gccacgactt
     2701 gctgtagatg tgtcaagtct gccgatgcat gctggtaatg ctacttacga tactggaagt
     2761 tcgtttgtta tccctcatat tgatcatatc catgtcgttc cgtattcatg gttgacgcgc
     2821 gatcagattg caacaatcaa gtatgtgatg caacaccccg aagttcgtcc ggatgtatgg
     2881 tctaagccag ggcatgaaga gtcaggttcg gtcattccaa atgttacgcc tcttgataaa
     2941 cgtgctggta tgccaaactg gcaaattatc cattctgctg aagaagttca aaaagcccta
     3001 gcagaaggtc gttttgcaac accagacggc tatattttcg atccacgaga tgttttggcc
     3061 aaagaaactt ttgtatggaa agatggctcc tttagcatcc caagagcaga tggcagttca
     3121 ttgagaacca ttaataaatc tgatctatcc caagctgagt ggcaacaagc tcaagagtta
     3181 ttggcaaaga aaaatgctgg tgatgctact gatacggata aacccaaaga aaagcaacag
     3241 gcagataaga gcaatgaaaa ccaacagcca agtgaagcca gtaaagaaga agaaaaagaa
     3301 tcagatgact ttatagacag tttaccagac tatggtctag atagagcaac cctagaagat
     3361 catatcaatc aattagcaca aaaagctaat attgatccta agtatctcat tttccaacca
     3421 gaaggtgtcc aattttataa taaaaatggt gaattggtaa cttatgatat caagacactt
     3481 caacaaataa acccttaacc aaaagaagat ctcattgtta aagcactgct ttgtcaaagc
     3541 aagttaccgt gattttgaag tcattttatg taacgagtag tgataaaagt tggataatag
     3601 cggttttctt ttgcaaagaa atggtatcca tgttagaata ataaaaaaaa gaggaggatt
     3661 ctaatcatgt cagaagaaaa attaaaatca aaaattgagc aagcatctgg tggcctaaaa
     3721 gaaggtgctg gtaagttgac aggtgataaa gagctagaag caaaaggatt tgttgaaaaa
     3781 acaattgcta aaggcaaaga acttgcagac gatgcaaaag aagcggttga aggtgctgta
     3841 gatgccgtta aggaaaaatt aaaataatta aaaaaaccag ctgtaagctg gtttttttaa
     3901 tggtgtgatt ttgttaggaa aagactcaat aggtaataac cgtaggtgtt aggtatgctt
     3961 gaacagttga gtacgattct cttcaatcat atagcacgcg tcacaaagtt tttctaacat
     4021 atcaaactca tgactaataa tgatgagacc taaatttcta tcctttacaa tctccaacag
     4081 gcttttccat acactagctt gtgtaatgct atccaacata gtagtcattt catctgcaat
     4141 aaggtatttc gtctctggat gtaatgaacg cacaatcgaa aagcgttgca attcccctcc
     4201 cgagagttca ctagggcgac gctttagcca tttttcttga atgccaaaag catctcgcaa
     4261 atcttgactt ggatagtagg cttcttccaa actttttttc ataggccata aaggattcat
     4321 ggtttgttca ggatgttgtt ggataagttg tacaggtcta aatgctttgt taggcaaatg
     4381 actgccatca actaatactt caccagattt aggctgcaaa aaaccggcaa gcactctgga
     4441 taaacttgtt tttccacacc cactttgtcc aaatatccct aagatttgac caggtgctac
     4501 ctctaaatca atctccttaa aaagccattg atctttttta tgataaaagc caagcttttt
     4561 agcctctaag gtcatgagta actcctttca aaaagtcttg ctgagggagt gtgcgccata
     4621 aacgtctcgc aaattctgtt tgtaattgct ccccacctcc gctaaaaaag ctagctgggg
     4681 ctgtttcaat agcttttccc tctttaaaaa tagttatacg atccgcaatt tgactagctg
     4741 ctacaatatc atgagtgata aatatgacgc ttattccttt atctgcaaag gagcgtagtt
     4801 gatctaaaac catttgcaga gcatctggat gtaatccagg ggtgggctca tccgcaataa
     4861 tcaaagaaac cgtatcacta atacacgttg taaacaaaac acgtcggagc attccgccag
     4921 aaagttggaa aggatacaag ttaccatcac tttcttttaa accaaactgt tgaaacaatc
     4981 cttcttgagt agccttagca ttttctgaga tacccaagcg cacctgatgc ttgactttca
     5041 tagatggatc taaataatta acggattgtg gaatcaacgt catttctttt cctcgcaact
     5101 gtttgatgcg tttagaagtt agtgattgac cacgataaat catatctcct gttacagctg
     5161 catttttagg aagaatatcc ataatagcat gtgctaataa actcttgcct gaaccactag
     5221 cacctataat agctaataac tcaccttttt taacttctaa attcagcgct tggatcggtg
     5281 tggattgaaa tggttttaaa aatcttccgt attgagtgaa ggtgatggag aggtctttaa
     5341 tgcttaataa tgtttctgtc atatctctac ctcctaaaaa tgatccgttt gagggtaaaa
     5401 gagtttcttt aaagattctc cgatagtatc aaaggcattg acaactaaaa taagataaag
     5461 gcctggaaaa accaccaacc accaattgcc aagagagata tgcttagctg cttctgacag
     5521 aatgatacca accgaaggtt gttcggcaga aaggccaaat cctaagaaag tcatggatgc
     5581 ttcatgcaag atgacgtgcg gaaataagag gataaaccca atgaaaattt gagaagcaat
     5641 caaaggcagg atatgatgcc tcacaatata ataaggcgtt tttcccatgc ttttagagag
     5701 ctggacaaag gctttattct ttagatcata gacttcattg cggataagcc ttgctagaga
     5761 gggccaatgg gtaacagccg ttgcaatgat aaccccttga gcccctttac caacaacaaa
     5821 agaaatgaga atcataaaaa tcaaatgagg cataccaata aacaaatcaa ccaaccaggc
     5881 tattatttta tcaataaggc tatttcctag acctgcaagc actccaaaaa cggtcgcaag
     5941 agagactccc ataagggcac ctaataagcc gacttgtaaa gagaaataaa gccctttaat
     6001 cgttctgacg aacatatccc tacctaaacc atctgtccca aaaagatggt ttaaggaagg
     6061 agcgaggttg cgtaaagatg cattggtttc caaaggcgtc ctatagaaat aaaggtttaa
     6121 ggctagaata ctaagaatga gagaaatggc gatacctagt tgccataaaa ccatcgttcg
     6181 acgtttcaat atcatacttt tctccttaac tgtggattga tgatgctatt taaaatatcc
     6241 gcaataagat tgcccgcaaa aacaaataat gtccctatca tcacgatagc tagaaggagc
     6301 ggtgtatcac ttttaagtcc tgcttcagtg agggtagacc ctaagccagg atatgagaaa
     6361 acttgctcag caagaacaga tcctccaaac aattctccaa aataggaaaa atgcagtgta
     6421 atagctggta cgatagcatt tctaaggcaa tgatgtttaa aaatttgcca ctgtgtttcc
     6481 ccacgcgctc tggcaaataa gacatattca ctagaaagca ccgacatcat tttagttctc
     6541 gtatgaaggg tgacattggc aatgcctaga atacttaacg tgaaaacagg taacataagg
     6601 tgcttaaccc gatcagctaa cgtaatatct tgactcaaag tgcctatcgg ggaagaaata
     6661 ccaatcggga accaccccag ctggacagaa aagattaata aaaaaatgag gccaatccaa
     6721 aacgttggta ctgatatctg aaggtaagaa aaccacctga caactcgatc aagtaatttc
     6781 ccttgatgga aagcggataa cgttcctaag ataaatccaa taagacccga taagatccaa
     6841 gagagtccca taagtatgaa agaagcacct gcccgtgatc taataatatc actaacaggt
     6901 tgccgataaa cgagcgaggt ccctaaatgt ccctgtatca catttttcaa ccaaataaaa
     6961 tactggacta gagctggctt atccaagcca tagtggtgag caatcgcttt gtactgagca
     7021 ggggttagtg atgtgtcata attgacactt gccatgactg gatctactgg agattgtttt
     7081 aacaaaacga aggtcaaaac agatacccca aaaataagcg tgacacatct gatgattttc
     7141 cagatgataa taatggtagt acgtttcaca atgaaatctc cttaatcgat aagaaaagga
     7201 gccgaggaaa cgatcagcac agttttgtgt atcttgtttt ctcagactcc aaacttaaag
     7261 tcgctgtatt aactaaacgc gttacttagt tgattcatcc caagtccact cggcaatgtt
     7321 agtcaataat gaccaatcat gaccatgact gtggacgccc tgtttaccta tattgatacg
     7381 tttatcacca atataagtat ggttaaggct caccaaccat acatttggca aatctccaag
     7441 agtagaagca cctgttttgc catcccactg cgctaacttc caatattcgt tagctttgtc
     7501 aaggtcagaa gatgtcattg ctttgtcaag gtacttagtc acggtaggat tgttataaaa
     7561 cgtaatattg gtccaacctt tacctgctag gcttggatga tgtgattcat aaaattgctg
     7621 cgcgtgatga cgtcctccgg cataaagtaa ggctgagtca tgtgacttcg ttgccatttc
     7681 atcccagtta ctagctttga gtttaatagt aatccctagg gctttggctt gctctgctac
     7741 ttcaacggct aagttcgctc gcaattgatc attagtaggg tagtacagat caaacgctgc
     7801 atcaaggtca ccttttttac ggctaccgtc tgcttgttct ttccatccag cttttgtcaa
     7861 caattgctta gctttagcta ctttattatc tttaatggct gtttttggat tccaaaatgg
     7921 tgttttatca ataattgaat aagctggttt accataacca tttaaaaccg tatcgagaac
     7981 tttttgccta tttaaaccaa tagtcaaggc ttttcggatt gctggatcac tagtgacatc
     8041 atttcctaca ggataaccat caggagaatc agtgatgacg ccttttttca cataaggtaa
     8101 tgataagccg cgcacatcat ttgatggaat atcaaggagg cgggtgcctt tgactttttt
     8161 atcagcaagt tctggcgttg cgtagatcat atcaacatca ccagattcta aagctgctag
     8221 tgctgtgttt tcatcaagta agacccaagt ccatttttta aagtatggtt ttttcccatg
     8281 ccaataaggg ttacgaacaa aaatagcttg ttctccagcc ttatattctt ttaccatgta
     8341 aggtcctgaa ccgataggat tgctcttata cttatcattg taatgttttt tagggacgat
     8401 tgggatttca gtcaactgtg ctgtaaatgt cgaatgcgcc tcagtcaaat ggatattgat
     8461 ctgatttttc ccaactactt caacgttctt gatgaaggtt agatcccaag cctttccatc
     8521 tgctttcaac atatcataag taaacttaac atcatcagca gtaacaggct caccatttga
     8581 gaatttaaaa tcatcatgca agtcaaacga ccaagtcagc ccatcttcag agagatggta
     8641 tgttttagca agctctcctt ttatatctag ttcaggagaa cgtttcaata gagtgctatg
     8701 agtgatattc ccttcattgt ggactccata acggtccttt ggatcgaatt catgagggag
     8761 ctttgccccc atagaaacga caagttcgtc ttttggacgt tgtttgcgct gacgttcttt
     8821 tgtttgaggc ttttgttgtt gacatgcaac taaaataagc ccagtcaaaa ataacgtgat
     8881 aatagagaag tattttaggt attttgacac aatcaaatct ccttatttct aattaataaa
     8941 taataatagt aacgactcca tctcattata gcaataaaca aataggaggg caattgttta
     9001 actggttaat tagaattttt gttattttta ggaagatact caggactttt ttataagttg
     9061 atgtattaac aaaagataaa gaggtttcat gacttatttt agattaaaaa aagaattaaa
     9121 ctgatcacta agaaataaag gaattgtagt ttacgaaggg aatcaaaaac ttcagatagc
     9181 cattaataca ggtaaaaata acagtgaaaa aagtcttttt tgatactttt tttgaggtgg
     9241 ttcgggcggc agcgactttc tccacaggaa aaaacatctg ttttggtctt ggcttacttc
     9301 gttcgcagaa ataggaaatc gggtatcaca ttagcagagt agttcatgtt ttttgagcat
     9361 cacactggat ctgtctacgt tctaaaagct ttcttcattt ttgtatcata ccccctacaa
     9421 gtagaaaaag tataacaaag aaaatttata aa
//
LOCUS       DATXIG010000050         9198 bp    DNA     linear   BCT 28-DEC-2023
DEFINITION  TPA_asm: Streptococcus pyogenes strain MGAS11113
            SAMN08555799-rid19682583.denovo.050, whole genome shotgun sequence.
ACCESSION   DATXIG010000050 DATXIG010000000
VERSION     DATXIG010000050.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN08555799
            Sequence Read Archive: SRR6740495
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pyogenes
  ORGANISM  Streptococcus pyogenes
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 9198)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9198)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-OCT-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-OCT-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001918058.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001918058.2
            Genome Coverage       :: 368x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/12/2023 11:23:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 1,806
            CDSs (total)                      :: 1,769
            Genes (coding)                    :: 1,722
            CDSs (with protein)               :: 1,722
            Genes (RNA)                       :: 37
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1, 1 (5S, 23S)
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 47
            CDSs (without protein)            :: 47
            Pseudo Genes (ambiguous residues) :: 0 of 47
            Pseudo Genes (frameshifted)       :: 30 of 47
            Pseudo Genes (incomplete)         :: 20 of 47
            Pseudo Genes (internal stop)      :: 9 of 47
            Pseudo Genes (multiple problems)  :: 11 of 47
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9198
                     /organism="Streptococcus pyogenes"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN08555799-rid19682583.denovo.050"
                     /strain="MGAS11113"
                     /serotype="Emm28"
                     /isolation_source="normally sterile site"
                     /host="Homo sapiens"
                     /db_xref="taxon:1314"
                     /country="Finland"
                     /collection_date="2002"
                     /collected_by="National Public Health Laboratory"
     gene            410..892
                     /locus_tag="VC122_001352"
     CDS             410..892
                     /locus_tag="VC122_001352"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010922334.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PASTA domain-containing protein"
                     /protein_id="HEP5641274.1"
                     /translation="MPKRIRKALGVVGKLMSIVPDTTEIIGKTIDNSRPIIEKRMEQK
                     HEKEMQLRTIDDVINLPVDQAQAHLEQLGFVVATIPAKPHKKWLHSNLNEVVAMSPKS
                     GKYKIGSLIKLYYITVDVLEKSQDLLDQENLRTVERNQKIADTFESVKHIRFPFKKWR
                     "
     gene            981..2033
                     /gene="citC"
                     /locus_tag="VC122_001353"
     CDS             981..2033
                     /gene="citC"
                     /locus_tag="VC122_001353"
                     /EC_number="6.2.1.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012560726.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="[citrate (pro-3S)-lyase] ligase"
                     /protein_id="HEP5641275.1"
                     /translation="MPYYTISKVFPSDKTTMASVKNLLHQEGIRLDAHLDYTCAIMNA
                     QNDVIATGSYFGNSLRCLCVSSAYQGGGFLNRIVSHLIDEEYALGNYHLFVYTKTSSA
                     AFFKDLGFTEIVHIDNHISFLENKKTGFQDYLMTLNKPEQTPGKVAAIVINANPFTLG
                     HQFLVEKAARENDWVHLFMVSEDQSLVPFSVRKMLIQEGLKHLDNIIFHETGPYLISQ
                     ATFPAYFQTEDNDVIKSQALLDTAIFLKIAQTLQITKRYVGEEPTSRVTAIYNEIMAE
                     QLQQAGILLDILPRKAINQQQDPISASTARQALKDSDWDLLAKLLPKTSLDYFCSLEA
                     QPIIKKIQATSSVKHY"
     gene            complement(2143..3537)
                     /locus_tag="VC122_001354"
     CDS             complement(2143..3537)
                     /locus_tag="VC122_001354"
                     /EC_number="4.1.1.112"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011285541.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oxaloacetate decarboxylase subunit alpha"
                     /protein_id="HEP5641276.1"
                     /translation="MTKIRITETVLRDGQQSQIATRMTTKEMIPILETLDNAGYHALE
                     MWGGATFDSCLRFLNEDPWERLRAIRKAVKKTKLQMLLRGQNLLGYRNYADDVVRSFV
                     QKSIENGIDIVRIFDALNDPRNLQTAVSATKKFGGHAQVAISYTTSPVHTIDYFVELA
                     KAYQAIGADSICIKDMAGVLTPEIGYQLVKCIKENTTIPLEVHTHATSGISEMTYLKV
                     AEAGADIIDTAISSFSGGTSQPATESMAIALTDLGFDTGLDMQEVAKVAEYFNTIRDH
                     YREIGILNPKVKDTEPKTLIYQVPGGMLSNLLSQLTEQGLADKYEEVLAEVPKVRADL
                     GYPPLVTPLSQMVGTQALMNIISGERYKVVPNEIKDYVRGLYGQSPAPLAEGIKEKII
                     GDEAVIACRPADLIEPQMIYLRDEIAQYARSEEDVLSYASFPQQARDFLGRREDPFYD
                     VPVQEVTVQLDIQD"
     gene            complement(3552..4130)
                     /gene="citX"
                     /locus_tag="VC122_001355"
     CDS             complement(3552..4130)
                     /gene="citX"
                     /locus_tag="VC122_001355"
                     /EC_number="2.7.7.61"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002991773.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="citrate lyase holo-[acyl-carrier protein]
                     synthase"
                     /protein_id="HEP5641277.1"
                     /translation="MCKDTYFSGEAIQLSDMLRAREERALRQLHLLKEYPEGSLLSVT
                     MNIPGPIKTSPKLLEVFDIVIKAIQTALADDKICYQLRLLPTTGYEYYLITSLPSRDL
                     KLKMIALETELPIGRLMDLDVLVLQNDLPHSISRTVLGGSPRQCFICSKEAKVCGRLR
                     KHSVEEMQTAISKLLHSFFNKDNQSSSSDKTG"
     gene            complement(4123..5655)
                     /gene="citF"
                     /locus_tag="VC122_001356"
     CDS             complement(4123..5655)
                     /gene="citF"
                     /locus_tag="VC122_001356"
                     /EC_number="2.8.3.10"
                     /EC_number="4.1.3.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014407592.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="citrate lyase subunit alpha"
                     /protein_id="HEP5641278.1"
                     /translation="MVTNKLGRDIPQPYADQYGVFEGELANIKQYDESSRRIKPVKPG
                     DSKLLGSVREAIEKTGLTDGMTISFHHHFREGDFIMNMVLEEIAKMGIKNLSIAPSSI
                     ANVHEPLIDHIKNGVVTNITSSGLRDKVGAAISEGLMENPVVIRSHGGRARAIASGDI
                     HIDVAFLGAPSSDAYGNVNGTKGKATCGSLGYAMIDAKYADQVVILTDNLVPYPNTPI
                     SIPQTDVDYVVTVDAIGDPQGIAKGATRFTKNPKELLIAEYAAKVITNSPYFKEGFSF
                     QTGTGGASLAVTRFMREAMIKENIKASFALGGITNAMVELLEEGLVEKILDVQDFDHP
                     SAVSLGKHADHYEIDANMYASPLSKGAVINQLDTCILSALEVDTNFNVNVMTGSDGVI
                     RGASGGHCDTAFAAKMSLVISPLIRGRIPTFVDEVNTVITPGTSVDVVVTEVGIAINP
                     NRQDLVDHFKSLNVPQFSIEELKEKAYAIVGTPERIQYGDKVVALIEYRDGSLMDVVY
                     NV"
     gene            complement(5658..6545)
                     /gene="citE"
                     /locus_tag="VC122_001357"
     CDS             complement(5658..6545)
                     /gene="citE"
                     /locus_tag="VC122_001357"
                     /EC_number="4.1.3.34"
                     /EC_number="4.1.3.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018380328.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="citrate (pro-3S)-lyase subunit beta"
                     /protein_id="HEP5641279.1"
                     /translation="MERLRRTMMFVPGANAAMLRDAPLFGADSIMFDLEDSVSLKEKD
                     TSRALVHFALKTFDYSSVETVVRVNGLDSCGALDIEAVVLAGVNVIRLPKTETAQDIV
                     DVEAVIERVERENSIEVGRTRMMAAIESAEGVLNAREIAKASKRLIGIALGAEDYVTN
                     MKTRRYPDGQELFFARSMILHAARAAGIAAIDTVYSDVNNTEGFQNEVRMIKQLGFDG
                     KSVINPRQIPLVNEIYTPTEKEIDHSKQVIWAIREAESKGSGVISLNGKMVDKPIVER
                     AERVIALATAAGVLSEEDI"
     gene            complement(6533..6841)
                     /gene="citD"
                     /locus_tag="VC122_001358"
     CDS             complement(6533..6841)
                     /gene="citD"
                     /locus_tag="VC122_001358"
                     /EC_number="4.1.3.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011284806.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="citrate lyase acyl carrier protein"
                     /protein_id="HEP5641280.1"
                     /translation="MDIKQTAVAGSLESSDLMITVSPNDEKTITITLDSSVEKQFGNH
                     IRQLIHQTLVNLKVTAAKVEAVDKGALDCTIQARTIAAVHRAAGVDQYDWKEIDSWNV
                     "
     gene            complement(7029..8150)
                     /locus_tag="VC122_001359"
     CDS             complement(7029..8150)
                     /locus_tag="VC122_001359"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019323213.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sodium ion-translocating decarboxylase subunit
                     beta"
                     /protein_id="HEP5641281.1"
                     /translation="MDTLIAGITSITIPQIVMMVIGALLMYLGIKKEYEPTLLVPMGL
                     GTILVNFPGSGVLTQVVNGVEQEGVFEALFNFGIGTELFPLLIFIGIGAMIDFGPLLQ
                     NPFMLLFGAAAQFGIFFVVVVAVLAGFDIKEAASIGIIGAADGPTSIFVANQLAKDLL
                     GPITVAAYSYMALVPIIQPFAIKLVTTKKERRIRMTYKAENVSQMTKILFPIIITLVA
                     GFIAPISLPLVGFLMFGNLLRECGVLDRLSQTAQNELVNIISILLGLTISIKMQADLF
                     LNVQTLLIIVFGLLAFIMDSIGGVMFAKFLNLFRKEKINPMIGAAGISAFPMSSRVIQ
                     KMATDEDPQNFILMYAVGANVSGQIASVIAGGLLLAYFS"
     gene            complement(8195..8590)
                     /locus_tag="VC122_001360"
     CDS             complement(8195..8590)
                     /locus_tag="VC122_001360"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014635472.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA carboxylase biotin carboxyl carrier
                     protein subunit"
                     /protein_id="HEP5641282.1"
                     /translation="MLRKFKITIDGKEYLVEMEEIGAPAQAAAPAQPISTPVPVPTEA
                     SPQVEEAQAPQPVAAAGADAIPSPMPGTILKVLVAVGDQVTENQPLLILEAMKMENEI
                     VASSAGTITAIHVGPGQVVNPGDGLITIG"
     gene            complement(8647..8970)
                     /locus_tag="VC122_001361"
     CDS             complement(8647..8970)
                     /locus_tag="VC122_001361"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002991762.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEP5641283.1"
                     /translation="MQFLQKWFKHSKKEKLKESSPLSTEIEPSENWEKIPAYIPADKS
                     DYKKVTLITSAIAAGDRPNSQFKVKRILKRNPEAITVSLIASSIAAGVYPESQFRVTS
                     IYCKR"
BASE COUNT         2721 a         1880 c         1823 g         2774 t
ORIGIN      
        1 cttttaggat tattcttgtt aaactactta atgtcattac tgataactta gagtaatata
       61 aagctaaaat tgaaggaata ttagagaatc atgaagacag cttctttaac atcaattggt
      121 tgaaaacaag aatcagataa cattatcgtt agatagactg aagaatcaaa aacacagttt
      181 gttacataaa taaattcttg tcaaaatttg tattttctat ttgaactgat acgattgaca
      241 aaggctaaac taaaatagct tgactttttt agaatctttg tgttttttgt cggctcccct
      301 cctatttttt ccttgaattg atctatttaa ccttttagaa caaggtttat cgcatactat
      361 ttttagacat tttgttataa taaaaagaaa cgacttgagg agataaaaga tgccaaagag
      421 aataagaaaa gctctaggag tagttgggaa actgatgtca attgttcctg acactactga
      481 aattattgga aaaacgattg ataacagtcg accaattatc gaaaaacgta tggaacaaaa
      541 acatgaaaaa gagatgcaac tacgcacgat agatgatgtg attaatcttc ctgttgatca
      601 ggcacaagct catttggaac aacttggctt tgtagtagcc actatacctg ctaagcctca
      661 taaaaaatgg cttcatagta atctcaatga agtggttgct atgtctccaa aatctggtaa
      721 atataagata ggaagcctga tcaaactcta ctacattact gttgatgttt tggaaaaaag
      781 tcaagactta ttagaccaag aaaacttgcg gactgtggaa cgcaatcaaa aaatcgctga
      841 tacttttgag tctgtcaaac acattcgatt tccttttaaa aaatggcgtt gataaaaaac
      901 agagttgcct ttagatagat cagcctgttt tggtactaag ttatgttatg attcaataaa
      961 attaaagata cggagaagaa atgccttact acactatttc aaaagtcttt ccttcagata
     1021 aaacgacaat ggctagtgta aagaacctgc ttcatcaaga aggtattcgt ttggatgctc
     1081 atttagatta tacttgtgcc atcatgaatg cgcaaaatga tgtgattgct accggttctt
     1141 actttggaaa tagcttgcgc tgtctttgcg tatccagtgc ctatcaaggt ggaggatttc
     1201 ttaataggat tgtgagtcat ctcattgatg aagaatatgc ccttggcaac tatcatcttt
     1261 ttgtttatac gaagacctct tctgctgctt ttttcaaaga tcttggtttt actgaaattg
     1321 tccatatcga caatcacatc agtttcttgg aaaataaaaa aactggtttt caagattatc
     1381 tgatgacact taataagcct gagcaaacac ctggtaaagt cgctgctatt gttatcaacg
     1441 ctaacccctt taccttaggg catcaatttt tagtagagaa agctgcaaga gaaaatgatt
     1501 gggttcatct gtttatggtc agtgaggacc aaagcttggt tcctttttca gtacgaaaaa
     1561 tgttgattca agaaggattg aaacatcttg ataacattat ctttcatgaa acaggtcctt
     1621 atttgattag tcaggcaacc tttccagctt atttccaaac ggaagacaat gatgtgatca
     1681 aaagccaagc tttgctggat actgctattt ttctaaagat tgctcaaacc ttacagatta
     1741 caaaaagata cgttggagaa gagccaacca gccgagtaac tgctatttac aatgaaatca
     1801 tggcagagca gttgcagcaa gctggtatcc tcctagatat tttgccaagg aaagctatca
     1861 atcagcagca agatcctatc agtgcctcaa cggctaggca ggcattgaaa gatagtgatt
     1921 gggaccttct ggcaaaactc cttcccaaaa cgtctttaga ttatttttgt tcgctagaag
     1981 cccaacccat tattaaaaag atacaagcca cttcatctgt caaacattac taagataaaa
     2041 aacctatctg attatcaaaa tcaggtaggt ttttggtatt gttcttacag caaatgcgtt
     2101 tcaccaagga gattaatggt taagaggatt agtcattccc tgttagtctt gaatgtccag
     2161 ttgtacagta acttcttgaa ccggaacatc atagaaagga tcttcacggc gtcccaagaa
     2221 atctctggct tgctgtggga aactcgcata gcttaacacg tcttcttctg aacgagcgta
     2281 ttgagcaatc tcgtcacgta gataaatcat ttgaggctcg attaagtcag ctggtctgca
     2341 agcaatgact gcttcgtcac caatgatttt ctctttgata ccttctgcta atggggctgg
     2401 tgattgaccg tatagccctc gaacatagtc tttgatttca tttggaacta ccttgtagcg
     2461 ttcccctgag atgatattca tcaaggcctg tgttccaacc atttgtgata atggcgttac
     2521 gagtggcgga tacccaagat cagcccttac tttaggtact tctgctaaga cttcttcgta
     2581 tttatcagct aggccttgct cggttaattg gctcaatagg tttgatagca tgccacctgg
     2641 aacttggtaa atcaatgttt ttggctcagt atctttgact ttaggattta aaatccctat
     2701 ttctcgatag tggtcacgaa tagtgttgaa atattctgca actttggcca cctcttgcat
     2761 atccaagcct gtgtcaaagc ctaaatccgt caaggcaatc gccattgact ctgtggcagg
     2821 ctgactggtt ccccctgaaa aagaggaaat cgccgtatca ataatatcag ctcctgcttc
     2881 tgctactttt aagtaagtca tttctgaaat accactggta gcatgggtat gaacctcaag
     2941 aggaatagtt gtattctcct tgatacattt gaccaattgg taacctattt caggagttaa
     3001 gacaccagcc atgtctttga tacaaatgga gtccgctccg atagcttggt aagctttcgc
     3061 caattcaaca aagtagtcaa tggtatgtac cggacttgtc gtgtaagaaa tggcaacttg
     3121 agcatgccct ccaaattttt tagtcgctga aacagctgtt tgcaagttgc gagggtcatt
     3181 taaagcatcg aagatacgga caatatcaat cccgttttca atggattttt gaacgaagga
     3241 tctgaccaca tcgtcagcat aattgcgata tccaagaagg ttttgtccgc gtaagagcat
     3301 ctgaagttta gtctttttca ctgctttacg gatggctctt agccgttccc aagggtcttc
     3361 atttaaaaag cgtaggcagg aatcaaaggt tgctcctccc cacatttcaa gggcatgata
     3421 accagcattg tctagcgttt cgagaatagg aatcatttcc ttggttgtca tacgtgtggc
     3481 aatctgactt tgttgaccat cacgtaaaac tgtttccgtt atacggattt tcgtcataat
     3541 tgttctcctc atcaacctgt cttatctgat gacgatgatt ggttgtcttt attgaaaaat
     3601 gaatggagta attttgaaat agcagtctgc atctcctcga cactgtgctt acgtaggcga
     3661 ccgcagactt tggcctcttt agaacagata aaacattgcc taggggagcc tcctaatacg
     3721 gttctgctaa ttgaatgagg cagatcattt tgcaagacca agacatctaa atccatgaga
     3781 cgacctattg gcaactctgt ctctaaggct atcattttta acttcaggtc gcggctaggt
     3841 agacttgtga tgaggtaata ctcataaccc gttgtaggca gtaatcgcaa ctggtaacaa
     3901 atcttatcgt cagctaaggc agtttgaatg gccttaatca ctatatcaaa aacttcaaga
     3961 agtttaggag aggttttaat tggtccaggg atattcatgg tgaccgataa taagctacct
     4021 tctgggtact cctttaataa atgcagctga cgcagagctc tttcttcgcg ggctcttaac
     4081 atatcactaa gttggatagc ttcgcctgaa aaataagtgt ctttacacat tgtaaaccac
     4141 atccatgagg ctaccatcac ggtattcaat aagagcaaca accttatcac catattgaat
     4201 acgctcaggt gtgccaacga tagcgtaagc cttctctttt agctcctcaa tactaaattg
     4261 tggcacattg agggatttga aatggtctac taagtcttga cgatttgggt taatggcaat
     4321 tcccacttct gtgacgacca catcgacact tgttcctggt gtaatgaccg tattaacctc
     4381 atctacaaaa gttgggatgc gtccgcggat aagtggtgaa ataaccaaac tcatcttagc
     4441 cgcaaaggca gtgtcgcagt gtcctccaga agcgccacga atcacgccgt cagatcctgt
     4501 catcacatta acattgaagt tagtatcgac ttcaagggct gataaaatac aagtgtctaa
     4561 ttgattgata acagcaccct tgctcaaagg tgaggcatac atgttggcat caatttcata
     4621 atggtcagca tgcttaccaa gagaaacagc tgatgggtga tcaaagtctt ggacatcaag
     4681 aattttttca accagtcctt cttcgagcaa ctccaccata gcattggtaa taccaccaag
     4741 agcaaagcta gctttgatat tttctttaat catagcttca cgcatgaaac gggttactgc
     4801 caaagaagcg ccacctgttc cagtttggaa agagaatcct tctttaaaat aaggagagtt
     4861 ggtgattact ttagcggcgt attctgcaat taagagttct ttaggatttt tcgtaaaacg
     4921 agttgcccct ttggcgattc cttgaggatc tccgatagca tccactgtta ccacatagtc
     4981 aacatctgtt tgaggaatgc tgattggggt attaggataa ggaaccaaat tatctgtcaa
     5041 gataaccacc tgatccgcat atttggcatc aatcatggca tagcccaaag aaccacaggt
     5101 tgctttccct tttgtcccat taacatttcc gtaagcatct gaacttgggg cgcccagaaa
     5161 ggcaacatca atatggatat ccccactagc aatggcacga gcgcgaccac catgggagcg
     5221 aataaccaca ggattttcca ttagtccttc tgagatggct gcccctactt tgtcacgaag
     5281 accagatgaa gtgatgttag tgaccacacc atttttgatg tgatcaatca aaggctcatg
     5341 tacattagcg atggaacttg gggcaataga caggttttta atgcccattt tggcaatttc
     5401 ctccaagacc atattcataa tgaaatcccc ttcacggaaa tggtggtgga aagaaatggt
     5461 catgccatct gttaaacctg ttttttcaat cgcttcacga acagatccta gtaatttaga
     5521 atctccaggc ttaactggtt taatacggcg acttgattcg tcatactgtt tgatatttgc
     5581 gagttctcct tcaaagacac cgtattggtc ggcgtatggt tgaggaatat ctctaccgag
     5641 tttatttgtt accatcttta aatgtcctcc tcagataaaa cacctgctgc tgttgctagg
     5701 gcaatgacac gttctgcacg ttcaacgatt ggtttatcaa ccatttttcc atttaaggaa
     5761 ataacgccag agcctttgct ttcagcttca cgaattgccc agatgacttg tttggaatgg
     5821 tcaatttctt tttcggttgg ggtataaatc tcattgacca gaggaatttg gcgagggttg
     5881 atgaccgatt taccgtcaaa tcctaactgt ttgatcatac gaacttcgtt ttggaatcct
     5941 tctgtattat tgacatcaga ataaacagta tcaatggcag caattccagc agcacgagca
     6001 gcatgtaaaa tcatgctacg agcaaagaat aattcttgac catctgggta acgacgcgtt
     6061 ttcatattcg tgacatagtc ttctgcccca agagcaatac caatcaagcg cttagaagct
     6121 ttggcaatct cacgagcatt tagaacacct tcggctgatt caatggctgc catcatacgt
     6181 gtgcgaccaa cttcaatgct gttctcacgt tcgacacgtt caataacagc ctccacatca
     6241 acgatatctt gtgcagtctc tgtttttgga agacgaatca cgttgacacc tgccaaaaca
     6301 acagcttcga tgtctaaagc cccacaagaa tcaagaccat tcacacgaac aaccgtttca
     6361 acgctcgaat aatcaaaagt ttttagcgca aaatggacga gagctctaga agtgtctttt
     6421 tctttgagtg aaacagaatc ttccaagtca aacatgattg aatcagcacc aaacaaagga
     6481 gcatctcgaa gcatagcagc attggcacct ggcacaaaca tcattgttct tcttaaacgt
     6541 tccatgagtc aatctccttc caatcgtatt ggtcaacacc agctgcgcga tgaacagctg
     6601 cgatggttct cgcttgaatg gtgcaatcta aagcgccttt atcaacagcc tctactttag
     6661 cagccgtcac cttcaaattc actagggttt gatgaatgag ttgacgaatg tgattgccaa
     6721 attgtttttc aacactactg tctagggtta ttgtaatggt tttctcgtca ttaggggata
     6781 ctgtaatcat taggtcactt gattcaagtg aaccagcaac ggcagtttgt ttaatatcca
     6841 ttgcgtttac ctccttgatt ttcttaaatg atattgttat ttactaactg ggaaaagttt
     6901 gatcaggatc tctgcgacaa gatagagaat tcctaacacg ataaagacac ctgccatacc
     6961 aagcgccatt agctcaaagg aactaatcaa atggtccata ttaattgtca taagctttcc
     7021 ttcctttctt agctaaagta agctagcaac aaaccacctg cgataacaga ggcgatttgg
     7081 ccagagacat tggcacctac agcatacatg aggataaagt tttgcgggtc ttcatctgtt
     7141 gccatttttt gaatgacacg acttgacatt ggaaaagctg aaatacccgc agcgccaatc
     7201 attggattaa tcttctcttt tcggaagaga ttgagaaatt tggcaaacat gaccccaccg
     7261 atagaatcca tgataaaagc aagtaaacca aagacgataa ttaagagtgt ctgtacattt
     7321 aagaacaggt ctgcctgcat tttaatagag atggtcaatc caagcaagat actaataatg
     7381 tttaccaatt cattttgagc agtttgtgac aggcgatcga gcacaccgca ctcacgtaat
     7441 aagttaccga acatcaaaaa gccaactaaa ggtagagaaa ttggggcgat gaaccctgct
     7501 actaaggtaa tgatgattgg gaataagatt ttagtcattt gagatacatt ctcagctttg
     7561 taagtcatac ggatacggcg ctcttttttc gttgtcacta atttaatggc aaatggttgg
     7621 ataataggaa ccagagccat gtaagaatag gctgcaaccg taattggacc cagtaaatcc
     7681 ttggccagtt ggttggcaac aaagatagaa gtcggtccat ctgctgctcc gataatgccg
     7741 attgaagctg cttctttaat gtcaaagcca gccagtacag caacaacaac aacaaagaaa
     7801 atcccaaatt gcgctgcagc accaaacaag agcatgaatg ggttttgaag caagggacca
     7861 aagtcaatca tggcgccaat accaatgaaa atcaacagtg ggaaaagttc tgttccaatc
     7921 ccaaaattga agagagcctc aaaaacccct tcttgctcga ctccgttaac cacttgggtt
     7981 aaaactcctg aaccagggaa gttaacgaga atggttccaa gtcccatggg gacaagtaag
     8041 gttggttcgt attctttttt aattcctaga tacattaaga gggcaccgat gaccatcata
     8101 acaatttgag gaatggtgat ggaggttatt cctgcgatta aagtgtccac aaattcactt
     8161 ccttttctgt ctatttattc tagttatagt actattaacc aattgtaatg agaccatcac
     8221 caggattgac cacttgacct ggaccgacgt ggatagctgt aatggttcct gctgatgagg
     8281 ccacaatctc attttccatc ttcatggctt ctaaaatcaa tagtggctga ttttcagtta
     8341 cttggtctcc cactgctact aagactttta agatggttcc aggcattggt gatggaatgg
     8401 catccgctcc cgctgcagct actggctgtg gtgcttgcgc ttcctccact tgtgggcttg
     8461 cttcagtagg gacaggtact ggagtggata ttggttgagc gggggcagct gcttgggcag
     8521 gagcaccgat ttcttccatt tctacaagat attctttacc gtcaatggta attttaaatt
     8581 ttcgtaacat catttttctc ctatatgtta ttgtgtcatg agttcgtcta aagtatcgtt
     8641 caacgactac cttttgcaat agatggatgt cacccgaaac tgactttctg ggtaaacacc
     8701 tgcagcgata cttgaagcaa ttaaagaaac agtgattgcc tcaggattgc gcttgagaat
     8761 gcgtttaacc ttgaattgac tattaggtct atcgccagca gcaatagcac tggtaattaa
     8821 agtaaccttt ttgtaatcac ttttgtctgc aggaatataa gcgggaatct tttcccagtt
     8881 ttcagaaggt tctatttctg tactaagggg cgaggattct ttcaacttct cttttttaga
     8941 gtgtttaaac catttttgta aaaactgcat gagctacctc tttttcacat ttacctattt
     9001 agggaactag taagtttatt atatactacc aatctttttt gagatggctt ttttgtgttc
     9061 tttctaagag cgctttcaat gtttcataaa gtttttcaac agttaccttt tattttattt
     9121 ttaggtaact gttgaaaaac tgagatcaag atactgatag caagaaaagc ttgatctaac
     9181 taggttcatc attattat
//
LOCUS       DATXIG010000051         9042 bp    DNA     linear   BCT 28-DEC-2023
DEFINITION  TPA_asm: Streptococcus pyogenes strain MGAS11113
            SAMN08555799-rid19682583.denovo.051, whole genome shotgun sequence.
ACCESSION   DATXIG010000051 DATXIG010000000
VERSION     DATXIG010000051.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN08555799
            Sequence Read Archive: SRR6740495
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pyogenes
  ORGANISM  Streptococcus pyogenes
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 9042)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9042)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-OCT-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-OCT-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001918058.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001918058.2
            Genome Coverage       :: 368x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/12/2023 11:23:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 1,806
            CDSs (total)                      :: 1,769
            Genes (coding)                    :: 1,722
            CDSs (with protein)               :: 1,722
            Genes (RNA)                       :: 37
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1, 1 (5S, 23S)
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 47
            CDSs (without protein)            :: 47
            Pseudo Genes (ambiguous residues) :: 0 of 47
            Pseudo Genes (frameshifted)       :: 30 of 47
            Pseudo Genes (incomplete)         :: 20 of 47
            Pseudo Genes (internal stop)      :: 9 of 47
            Pseudo Genes (multiple problems)  :: 11 of 47
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9042
                     /organism="Streptococcus pyogenes"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN08555799-rid19682583.denovo.051"
                     /strain="MGAS11113"
                     /serotype="Emm28"
                     /isolation_source="normally sterile site"
                     /host="Homo sapiens"
                     /db_xref="taxon:1314"
                     /country="Finland"
                     /collection_date="2002"
                     /collected_by="National Public Health Laboratory"
     gene            complement(77..1390)
                     /locus_tag="VC122_001362"
     CDS             complement(77..1390)
                     /locus_tag="VC122_001362"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015017188.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chloride channel protein"
                     /protein_id="HEP5641284.1"
                     /translation="MTIIIMDSNSAHETDNLSVSFLNFCYNSLMKRHFLLLTFYLFLT
                     GLTAGLVAFILTKAIHLIQSLSFGFSQGSFSTMIASVPPQRRALSLLFAGLLAGLGWH
                     LLAKKGKDIQSIQQIIQDDISFSPWTQFWHGWLQLTTVSMGAPVGREGASREVAVTLT
                     SLWSQRCNLSKADQKLLLACASSAALGAVYNAPLATILFILEAILNRWSLKNIYAACL
                     TSYVAVETVALLQGRHEIQYLMPQQHWTLGTLIWSVLAGLILSLFAHAYKHLLEHLPK
                     ADAKSQWFIPKVLIAFSLIAGLSIFFPEILGNGKAGLLFFLHEEPHLSYISWLLVAKA
                     VAISLVFASGAKGGKIAPSMMLGGASGLLLAILSQYLIPLSLSNTLAIMVGATIFLGV
                     INKIPLAAPVFLVEITGQSLLMIIPLALANLIFYFSYQFYRFILK"
     gene            complement(1365..2324)
                     /gene="nrdF"
                     /locus_tag="VC122_001363"
     CDS             complement(1365..2324)
                     /gene="nrdF"
                     /locus_tag="VC122_001363"
                     /EC_number="1.17.4.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017646384.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class 1b ribonucleoside-diphosphate reductase
                     subunit beta"
                     /protein_id="HEP5641285.1"
                     /translation="MTTYYEAINWNEIEDVIDKSTWEKLTEQFWLDTRIPLSNDLDDW
                     RKLSLQEKDLVGKVFGGLTLLDTMQSETGVEAIRADVRTPHEEAVLNNIQFMESVHAK
                     SYSSIFSTLNTKKEIEEIFEWTNNNEFLQEKARIINNIYANGDALQKKVASTYLETFL
                     FYSGFFTPLYYLGNNKLANVAEIIKLIIRDESVHGTYIGYKFQLGFNELPEDEQENFR
                     DWMYDLLYQLYENEEKYTKTLYDGVGWTEEVMTFLRYNANKALMNLGQDPLFPDTAND
                     VNPIVMNGISTGTSNHDFFSQVGNGYLLGSVEAMSDDDYNYGL"
     gene            complement(2657..4816)
                     /gene="nrdE"
                     /locus_tag="VC122_001364"
     CDS             complement(2657..4816)
                     /gene="nrdE"
                     /locus_tag="VC122_001364"
                     /EC_number="1.17.4.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000053857.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class 1b ribonucleoside-diphosphate reductase
                     subunit alpha"
                     /protein_id="HEP5641286.1"
                     /translation="MSLKDIGDISYFRLNNEINRPVNGKIPLHKDKEALKAFFAENVL
                     PNTMSFTSITEKIEYLISNDYIESAFIQKYRPEFITELDSIIKSENFRFKSFMAAYKF
                     YQQYALKTNDGEHYLENLEDRVLFNALYFADGQEDLAKDLAVEMINQRYQPATPSFLN
                     AGRSRRGELVSCFLIQVTDDMNSIGRSINSALQLSRIGGGVGITLSNLREAGAPIKGY
                     AGAASGVVPVMKLFEDSFSYSNQLGQRQGAGVVYLNVFHPDIIAFLSTKKENADEKVR
                     VKTLSLGITVPDKFYELARKNEDMYLFSPYNVEKEYGIPFNYLDITNMYDELVANPKI
                     TKTKIKARDLETEISKLQQESGYPYIINIDTANKANPIDGKIIMSNLCSEILQVQTPS
                     LINDAQEFVEMGTDISCNLGSTNILNMMTSPDFGRSIKTMTRALTFVTDSSSIEAVPT
                     IKHGNSQAHTFGLGAMGLHSYLAQHHIEYGSPESIEFTDIYFMLLNYWTLVESNNIAR
                     ERQTTFVGFENSKYANGSYFDKYVTGHFVPKSDLVKSLFKDHFIPQASDWEALRDAVQ
                     KDGLYHQNRLAVAPNGSISYINDCSASIHPITQRIEERQEKKIGKIYYPANGLSTDTI
                     PYYTSAYDMDMRKVIDVYAAATEHVDQGLSLTLFLRSELPMELYEWKTQSKQTTRDLS
                     ILRNYAFNKGIKSIYYIRTFTDDGEEVGANQCESCVI"
     gene            complement(4836..5054)
                     /locus_tag="VC122_001365"
     CDS             complement(4836..5054)
                     /locus_tag="VC122_001365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011017931.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="redoxin NrdH"
                     /protein_id="HEP5641287.1"
                     /translation="MITVYSKNNCMQCKMTKKFLEQHGANFQEINIDEHPEKVDYVKS
                     LGFTSAPVIEADNLVFSGFQPAKLKELI"
     gene            5447..5710
                     /locus_tag="VC122_001366"
     CDS             5447..5710
                     /locus_tag="VC122_001366"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019775469.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphocarrier protein HPr"
                     /protein_id="HEP5641288.1"
                     /translation="MASKDFHIVAETGIHARPATLLVQTASKFASDITLDYKGKAVNL
                     KSIMGVMSLGVGQGADVTISAEGADAEDAIAAIEETMTKEGLA"
     gene            5715..7448
                     /gene="ptsP"
                     /locus_tag="VC122_001367"
     CDS             5715..7448
                     /gene="ptsP"
                     /locus_tag="VC122_001367"
                     /EC_number="2.7.3.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014635558.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoenolpyruvate--protein phosphotransferase"
                     /protein_id="HEP5641289.1"
                     /translation="MTEMLKGIAASDGVAVAKAYLLVQPDLSFETVTVADTNAEEARL
                     DVALQAAQDELSVIRENAVESLGEEAAAVFDAHLMVLADPEMISQVKETIRAKQTNAE
                     TGLKEVTDMFITIFEGMEDNPYMQERAADIRDVAKRVLAHLLGVKLPNPATINEESIV
                     IAHDLTPSDTAQLNKQFVKAFVTNIGGRTSHSAIMARTLEIAAVLGTNDITKRVKDGD
                     VIAVNGITGEVIIDPSEDQVLAFKEAGAAYAKQKAEWSLLKDAHTETADGKHFELAAN
                     IGTPKDVEGVNDNGAEAVGLYRTEFLYMDSQDFPTEDEQYEAYKAVLEGMNGKPVVVR
                     TMDIGGDKELPYFDLPKEMNPFLGFRALRISISETGDAMFRTQMRALLRASVHGQLRI
                     MFPMVALLKEFRAAKAVFDEEKANLLAEGVAVADDIQVGIMIEIPAAAMLADQFAKEV
                     DFFSIGTNDLIQYTMAADRMNEQVSYLYQPYNPSILRLINNVIKAAHAEGKWAGMCGE
                     MAGDQQAVPLLVGMGLDEFSMSATSVLRTRSLMKKLDSAKMEEYANRALTECSTAEEV
                     LELSKEYVSED"
     gene            7633..>9042
                     /locus_tag="VC122_001368"
     CDS             7633..>9042
                     /locus_tag="VC122_001368"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012515401.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADP-dependent glyceraldehyde-3-phosphate
                     dehydrogenase"
                     /protein_id="HEP5641290.1"
                     /translation="MAKQYKNLVNGEWKLSENEITIYAPATGEELGSVPAMTQAEVDA
                     VYASAKKALSDWRALSYVERAAYLHKAADILVRDAEKIGAILSKEVAKGHKAAVSEVI
                     RTAEIINYAAEEGLRMEGEVLEGGSFEAASKKKIAIVRREPVGLVLAISPFNYPVNLA
                     GSKIAPALISGNVVALKPPTQGSISGLLLAEAFAEAGIPAGVFNTITGRGSVIGDYIV
                     EHEAVNFINFTGSTPIGEGIGKLAGMRPIMLELGGKDSAIVLEDADLVLAAKNIVAGA
                     FGYSGQRCTAVKRVLVMDKVADQLAAEIKTLVEKLSVGMPEDDADITPLIDTSAADFV
                     EGLIKDATDKGATALTAFNREGNLISPVLFDHVTTDMRLAWEEPFGPVLPIIRVTTVE
                     EAIKISNESEYGLQASIFTTNFPKAFGIAEQLEVGTVHLNNKTQRGTDNFPFLGAKKS
                     GAGVQGVKYSIEAMTTVKSV"
BASE COUNT         2881 a         1632 c         1896 g         2633 t
ORIGIN      
        1 cacttaattt tgtgtctact ttagtatacc aagtccaatt catccgaagc tttttattaa
       61 aaaagtaatt aactaatcat ttaaggatga aacgataaaa ctgatagcta aaataaaaaa
      121 tgaggttagc taatgctaaa gggataatca ttagtaacga ttggcctgta atttcaacca
      181 gaaaaactgg tgccgccaag ggtatcttat tgatcacacc taaaaagatt gttgcaccaa
      241 ccataatggc taacgtattt gatagagaca agggaataag gtactgacta aggatagcta
      301 gtagtaagcc actagcacct ccaagcatca tagaaggagc aattttaccc ccttttgctc
      361 cagaagcaaa aactaaagag atagcgacag ctttggcaac aagtagccag ctaatatagc
      421 tcagatgtgg ttcttcatgg agaaaaaaga gtaatccagc tttgccattt cctaaaatct
      481 ctgggaagaa aatgctgagt cctgcgataa ggctaaaagc gatgagaact ttcggaataa
      541 accactgact tttggcatca gccttaggaa ggtgttccaa aagatgtttg taagcgtgag
      601 caaagaggga aaggataaga ccagccagga cggaccaaat aagggttcct agcgtccaat
      661 gttgctgagg cattaggtat tgaatctcat gtcggccttg taataaagca actgtttcca
      721 cagccacata gctcgttagg caggcagcgt atatattttt aagggaccaa cggttaagaa
      781 tagcttccaa gataaataaa atagtggcta agggagcatt gtagacagca ccaagggcag
      841 cgctagaagc gcaagctaat aaaagttttt gatcggcttt tgacaagttg catctttggc
      901 tccaaagaga cgtcaaggta acagcgactt cccgtgaagc tccttctcgg ccgacaggag
      961 ctcccataga tacggtcgtt aattggagcc aaccatgcca aaactgtgtc cagggactaa
     1021 atgagatgtc atcttgaatg atttgctgaa tagattgaat gtcctttcct tttttagcca
     1081 ataggtgcca cccaagacct gcaagaagtc cagcaaagag aagtgaaaga gctcgtcttt
     1141 gtggtggaac gctagcaatc atagtgctaa aagaaccttg actgaatcca aaactgaggg
     1201 actgaatcag gtgaatggct tttgttaaga taaaagcaac aaggccagct gttaaacctg
     1261 ttaaaaagag gtaaaaggtc aagagtaaaa agtgtcgttt cataagacta ttataacaaa
     1321 agttaagaaa agagactgat agattatcag tctcgtgagc actattagag tccataatta
     1381 taatcgtcat cagacatagc ttctacagaa ccaagaagat agccattccc tacctgtgag
     1441 aaaaagtcat ggtttgatgt tcctgttgaa ataccattca taacaattgg gttaacatcg
     1501 tttgcagtat ctgggaataa aggatcttgt cctaaattca taagagcttt gttagcattg
     1561 tagcgtaaaa aggtcataac ttcctcagtc caccctacgc catcataaag cgttttggtg
     1621 tatttttctt cattttcata cagctgataa aggaggtcat acatccaatc acgaaaattc
     1681 tcttgctcat cttctggtag ttcgttaaaa ccaagctgga atttgtaacc gatataagta
     1741 ccatgtacag attcatcacg aataattagt ttaatgattt cagcaacatt tgctagctta
     1801 ttatttccca aatagtaaag aggggtgaaa aagccagaat aaaaaaggaa agtttcgaga
     1861 tatgttgagg caactttctt ttgtaaggca tctccattag cgtaaatgtt attgataata
     1921 cgtgcttttt cttgaaggaa ctcattatta ttagtccact caaagatctc ttcaatttct
     1981 tttttggtgt ttaaggttga aaagatagac gagtatgatt tagcatgaac tgattccata
     2041 aattgaatat tgtttaagac agcttcttcg tgaggagtgc gaacatcagc acgaatagct
     2101 tcgacaccag tttctgattg catggtatca agtagggtta atcctccaaa aaccttgcca
     2161 acaagatctt tttcttgaag agaaagtttg cgccaatcgt ctaagtcatt tgataaaggg
     2221 atacgggtat caagccaaaa ttgttcggtc aacttttccc aagttgactt gtcaatgacg
     2281 tcttcgattt cattccagtt aattgcttca taatatgtag tcatagaaaa atgatctcct
     2341 aaagagtagt aaagtgcgtt aaggatatcg agataaacca gtagaaccga ccaaagttct
     2401 accggtaata ataagtttaa atagattcaa aagggtaacg aatgagtcag tgagtggttt
     2461 aggcggaacg cctaaagcga ttgtccaaga agtaatagat gctttagcat cattagggca
     2521 tatcgaggaa ttacgacacg gagtaaggaa agtcgataat actttcctta cgtagttagt
     2581 gagtggttta ggcagatcgc ctaaagcgac tgccgtagaa gtaatagatg ctttagcatc
     2641 attacttctt aaccaattaa atgacacaag attcacattg gtttgcgccc acttcttccc
     2701 catcatccgt aaaggtacgg atatagtaga tagatttaat gcctttattg aaagcgtagt
     2761 ttcgtaagat ggataaatca cgagtggttt gtttgctttg tgttttccac tcataaagct
     2821 ccataggcaa ctcactacga aggaatagag ttaatgacaa gccttggtcc acatgttcgg
     2881 tcgcagcggc atagacatca ataactttgc gcatgtccat atcgtaagca gatgtatagt
     2941 aaggaatggt atccgtagac aaaccatttg caggatagta gattttacca attttctttt
     3001 cttgacgttc ttcaattctt tgtgtgattg ggtgaataga agcagagcag tcattgatat
     3061 aagaaataga gccatttgga gcaactgcta gacggttttg atgataaaga ccatcttttt
     3121 gaacggcgtc gcgaagagcc tcccaatctg aagcttgcgg aataaagtga tctttaaaga
     3181 gacttttgac caaatcagat tttggaacaa agtgtcctgt aacgtattta tcaaagtaac
     3241 taccattagc gtacttagag ttctcaaagc caacaaaggt agtttggcgc tcacgagcga
     3301 tgttattgga ttcgaccaag gtccaataat tcaggagcat aaagtaaata tcagtaaact
     3361 cgatggattc tggactgcca tattcaatat gatgttgagc aaggtaagaa tgtagtccca
     3421 tagctccaag gccaaaagta tgagcttggc tattgccatg tttaatggtt ggaacagctt
     3481 caatgcttga tgaatcagta acaaaagtta gggcacgtgt catggtctta atagaacggc
     3541 caaagtctgg tgaggtcatc atgttcagga tattagtgga acctaagtta catgaaatat
     3601 cagttcccat ttctacaaac tcttgcgcat cattgataag gctaggtgtt tgaacttgta
     3661 aaatttcaga acacaagttg ctcatgatga tttttccatc gataggatta gctttattag
     3721 ctgtatcaat attgatgata taagggtaac cagattcttg ttgtaatttt gaaatctctg
     3781 tttcaagatc acgagcttta attttagtct tagtaatttt agggttcgcc actaactcat
     3841 cgtacatatt ggtaatgtcg agatagttaa agggaatgcc atattctttt tcaacattgt
     3901 aaggactaaa gagatacatg tcctcgtttt tacgagctaa ttcgtagaat ttatcaggaa
     3961 cggtaatccc tagtgacaag gttttaacac gcaccttttc atcggcattt tcttttttag
     4021 tagataagaa agcaatgata tcaggatgaa aaacatttag gtaaacaaca ccagctcctt
     4081 gacgttgccc aagttgattt gaataagaaa aactatcttc aaataatttc ataacaggaa
     4141 caactcctga ggctgcacca gcatagcctt tgattggtgc gccagcttca cggaggttag
     4201 acaaggtaat cccaacacct ccaccaatac gggataattg caaggcagag ttaatggaac
     4261 gtccgataga attcatgtca tcagttactt gaatcaagaa acaagagacc aattcaccac
     4321 gacggcttcg accagcattt aaaaaggaag gagtagccgg ttggtaacgt tggttaatca
     4381 tttcaacggc taaatctttt gctaagtctt cttgaccatc tgcaaaatac aaagcattaa
     4441 acaagacacg gtcttcaagg ttttctaaat aatgctctcc atcatttgtt tttaaggcgt
     4501 attgctggta gaacttgtag gctgccataa atgatttaaa gcgaaaattt tctgatttga
     4561 ttatgctatc taattcagta ataaattcag gacggtattt ctgaataaaa gctgattcaa
     4621 tgtaatcatt tgagattaaa tactcaattt tttccgtaat ggaagtaaaa gacatggtgt
     4681 ttggcagcac attttcagcg aaaaaagctt ttaaagcttc tttgtcttta tgaagtggaa
     4741 ttttaccatt aacaggacgg ttaatttcat tatttaggcg aaaatatgaa atatcgccaa
     4801 tatctttgag actcatatta tttttccttg tcttattaaa tcaattcttt taactttgca
     4861 ggttggaagc cagaaaatac gaggttatct gcttcgataa caggagcaga tgtgaagcca
     4921 agacttttca cataatccac tttttcagga tgttcatcga tattaatttc ctggaagttg
     4981 gctccgtgtt gttctaaaaa tttcttagtc attttgcact gcatgcaatt atttttggaa
     5041 tatactgtaa tcatgtcttg tccttctaaa attagtaatg gttccatctt aaatggaatt
     5101 actataatag catataaaaa ataaagctaa aaagcaagct ataaacttat ctaaaacaca
     5161 acatattgtg ttgaaataaa aaacagaaac ctaaatattg tgtcaatctg gattcttatg
     5221 gttagagctc tagcgttttg gtgatagaag ctaagcagtt ttaataaagc attaaagtct
     5281 gaaaaataaa ttgtgaaaaa cgtgaaaata aaagaaaata atttcacatt ttttgtcgtt
     5341 taaatattta atcttgaaaa taaaaaagca ttgtgctata atcggttgtt gtaagggtta
     5401 tcatgtgatg gctcaaaaat acacaactta aaaggagaac catactatgg cttcaaaaga
     5461 ctttcacatt gttgcagaaa caggtattca tgcgcgtcca gcgactttgc ttgttcaaac
     5521 agctagcaaa tttgcttcag acatcactct tgactacaaa ggtaaagcag taaatctaaa
     5581 atcaatcatg ggtgttatga gtcttggtgt tggtcaaggt gcagatgtta ctatctcagc
     5641 tgaaggtgca gatgctgaag atgccattgc agcaattgaa gaaacaatga ctaaagaggg
     5701 attggcataa gactatgaca gaaatgctta aaggaattgc agcctcagac ggcgttgctg
     5761 ttgctaaagc atatctacta gttcagccgg atttgtcatt tgagactgtt acagtcgcag
     5821 atacaaatgc agaagaagct cgccttgatg ttgcactcca agctgcacaa gacgagcttt
     5881 ctgttatccg tgaaaatgca gtagaaagct taggtgaaga agcagcagcc gtttttgatg
     5941 cccatttgat ggttcttgct gatccagaaa tgatcagcca ggttaaagaa acgattcgcg
     6001 caaaacaaac gaatgcagaa acaggtctta aagaagtgac tgacatgttc atcaccatct
     6061 ttgaaggcat ggaagataac ccatacatgc aagaacgtgc agcggacatc cgcgacgttg
     6121 caaaacgtgt gttggctcac cttttaggtg taaaacttcc aaatccagct acaatcaatg
     6181 aagaatcaat cgttatcgca cacgatttga caccttcaga tactgctcaa cttaacaaac
     6241 aatttgtaaa agcatttgtt acaaatatcg gtggtcgtac aagtcactca gctatcatgg
     6301 cacgtacact tgagatcgct gcggtacttg gaacaaatga tattacaaaa cgtgttaaag
     6361 atggtgatgt gattgccgtt aatggtatca ctggtgaagt gattatcgat ccaagcgaag
     6421 atcaagtact tgcttttaaa gaagctggtg cggcttatgc caaacaaaaa gcagagtggt
     6481 ctctccttaa agatgcgcac actgaaacag ctgatggcaa acactttgaa ttggctgcta
     6541 atatcggtac gcctaaagac gttgaaggtg ttaatgacaa tggcgctgaa gctgttggcc
     6601 tttaccgtac tgagttcttg tacatggatt ctcaagactt cccaactgaa gacgaacaat
     6661 acgaagctta caaagcagtg cttgaaggca tgaatggcaa acctgttgtg gttcgtacaa
     6721 tggatattgg tggagataag gaacttcctt actttgacct tccaaaagaa atgaacccat
     6781 tcctcggttt ccgtgctctt cgtatctcca tctctgaaac tggggatgcc atgttccgca
     6841 cacaaatgcg tgcgcttctt cgcgcctctg ttcacggaca acttcgtatc atgttcccaa
     6901 tggtagcact tcttaaagaa ttccgtgctg caaaagcagt ctttgacgaa gaaaaagcaa
     6961 acttgcttgc agaaggcgtt gcggttgctg atgacatcca agttggtatc atgattgaga
     7021 ttcctgcagc tgctatgctt gcagaccaat ttgctaagga agttgatttc ttctcaattg
     7081 gaacaaacga ccttatccaa tacactatgg cagcagaccg tatgaacgaa caagtatcat
     7141 acctttacca accatacaac ccatcaatat tacgtttgat caacaatgtg atcaaagcag
     7201 cgcacgctga aggtaaatgg gcaggtatgt gtggtgagat ggcaggtgac caacaagctg
     7261 ttccacttct tgttggaatg ggcttggatg agttttctat gtcagcaact tcagtacttc
     7321 gtacgcgtag tttaatgaag aaacttgact ctgctaagat ggaagaatat gcaaatcgtg
     7381 cgcttacaga atgttcaaca gcagaagaag ttcttgaact ttctaaagaa tacgtttctg
     7441 aagattaacg ggttaaagag ttggtttaaa ccagctcttt tttgctagct aaaaatacaa
     7501 ttactttttg gtgtgaaatc atttgagtta tcaaataatt atcagaattt tcagatattt
     7561 agtgtagcca aatcttgaat tatatcagaa aagtgataga ataagattac tatcataaaa
     7621 ggagataaaa cgttggcaaa acaatataaa aatttagtga acggtgaatg gaaactatca
     7681 gaaaacgaga ttaccattta cgcaccagca acaggtgaag agttaggatc agttccagcg
     7741 atgacgcagg cagaggtaga tgctgtttac gcttcagcta aaaaggctct atcagattgg
     7801 cgcgccttgt cttatgtgga acgtgcagct taccttcata aagcggctga tattttagta
     7861 cgtgatgctg aaaagatcgg cgcgattctt tcaaaagaag tagccaaagg tcacaaggca
     7921 gctgtcagtg aagttattcg taccgctgaa atcattaatt atgcagcaga agaagggctt
     7981 cgtatggaag gtgaagttct tgaaggtggt agcttcgaag ccgcaagtaa gaagaagatt
     8041 gctattgttc gtcgtgaacc agttggttta gttcttgcca tctcaccttt taattatccc
     8101 gttaacttgg caggttctaa aattgctcca gcccttattt caggaaatgt tgttgctctt
     8161 aaaccaccaa cacaaggctc tatttctggt ttgttactag cagaagcttt tgcagaagct
     8221 ggtattccag caggtgtctt taataccatt acagggcgag gttctgttat tggggattac
     8281 atcgttgagc acgaagcggt taactttatc aactttacag gttctactcc aattggggaa
     8341 ggaatcggta aattagcggg tatgcgacca attatgcttg agcttggcgg taaggattct
     8401 gctatcgttt tggaagatgc agatcttgtt ttagcagcga aaaatattgt agccggtgct
     8461 tttggttact caggacaacg ttgtacagcg gttaaacgcg ttcttgtgat ggacaaggtg
     8521 gcggatcaat tggcggctga gattaaaaca cttgttgaaa aactaagtgt cggaatgcct
     8581 gaagacgatg ctgatattac accattaatt gatacatcag ctgctgattt tgttgaaggg
     8641 ttgattaaag atgcaactga taagggagct actgctttga cagcctttaa tcgtgaaggc
     8701 aatcttattt cacccgttct ctttgatcat gtgacaactg acatgcgttt ggcatgggaa
     8761 gagccgttcg gcccagtatt accaattatt cgtgtaacca ctgtagaaga agccatcaag
     8821 atttctaatg agtctgaata tggtttgcaa gcttctattt ttacaactaa tttcccaaaa
     8881 gcttttggca ttgctgagca attagaagtt ggaactgttc accttaacaa taaaacacaa
     8941 cgtggaacag ataatttccc attcttaggc gctaaaaaat caggtgcagg ggtacaagga
     9001 gttaaatatt ctatcgaagc tatgacaact gttaaatctg tt
//
LOCUS       DATXIG010000052         8638 bp    DNA     linear   BCT 28-DEC-2023
DEFINITION  TPA_asm: Streptococcus pyogenes strain MGAS11113
            SAMN08555799-rid19682583.denovo.052, whole genome shotgun sequence.
ACCESSION   DATXIG010000052 DATXIG010000000
VERSION     DATXIG010000052.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN08555799
            Sequence Read Archive: SRR6740495
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pyogenes
  ORGANISM  Streptococcus pyogenes
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 8638)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8638)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-OCT-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-OCT-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001918058.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001918058.2
            Genome Coverage       :: 368x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/12/2023 11:23:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 1,806
            CDSs (total)                      :: 1,769
            Genes (coding)                    :: 1,722
            CDSs (with protein)               :: 1,722
            Genes (RNA)                       :: 37
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1, 1 (5S, 23S)
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 47
            CDSs (without protein)            :: 47
            Pseudo Genes (ambiguous residues) :: 0 of 47
            Pseudo Genes (frameshifted)       :: 30 of 47
            Pseudo Genes (incomplete)         :: 20 of 47
            Pseudo Genes (internal stop)      :: 9 of 47
            Pseudo Genes (multiple problems)  :: 11 of 47
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8638
                     /organism="Streptococcus pyogenes"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN08555799-rid19682583.denovo.052"
                     /strain="MGAS11113"
                     /serotype="Emm28"
                     /isolation_source="normally sterile site"
                     /host="Homo sapiens"
                     /db_xref="taxon:1314"
                     /country="Finland"
                     /collection_date="2002"
                     /collected_by="National Public Health Laboratory"
     gene            <1..853
                     /locus_tag="VC122_001369"
     CDS             <1..853
                     /locus_tag="VC122_001369"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014407828.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEP5641291.1"
                     /translation="PEVNKEMIYINKSGANIQNKVNGTDYQLLDNKVATIYIPDKFKE
                     KKKSIENTVVAEQFTGTKYTKENLAVQIIPNEKKIFYFNENGNEHDENKEASPMANVT
                     DSKGNIVVVLDTDKMVASKDFSLASNIVNSSLFSPEAIKKINDLSLHLNFSINPVDVY
                     QLVKLNIQSLKHQIFLSKVLQKIIYGIVFLLIYQYAQLFISSKQNDYVKKIILGLSKT
                     RIAISSLKYFIMTIAIVIICTFMVTEQIELLYIGIASLVVLILSTVMSFRKLSKKYTQ
                     ILKGDEQ"
     gene            850..1471
                     /locus_tag="VC122_001370"
                     /pseudo
     CDS             850..1471
                     /locus_tag="VC122_001370"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002922687.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
     gene            1468..1959
                     /locus_tag="VC122_001371"
     CDS             1468..1959
                     /locus_tag="VC122_001371"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014407827.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEP5641292.1"
                     /translation="MKKKIYIGLIVIVGVFVVFLSGKQITQKETSNKPVVTLTVYTIS
                     SSDTQKWSKVKQFETKEAIYLVKVKETTSSEEVFSNIIADGMAVGFGVSEEDVKQFND
                     NLCSPVEDAKNNKLIGIEFINFSSVDKGFVVANFDYGKKAFNSQKTENKKLYKKLYEA
                     YKG"
     gene            2163..2693
                     /locus_tag="VC122_001372"
     CDS             2163..2693
                     /locus_tag="VC122_001372"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011018203.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF536 domain-containing protein"
                     /protein_id="HEP5641293.1"
                     /translation="MGIEKTVSELADILGVSRQAVNNRVKSLPEEDLDKNEKGVTVVK
                     RSGLVKLEEIYKKTIFDDEPISEETKQRELLEILVDEKNTEITRLYEQLKAKDAQLAS
                     KDEQMRVKDVQIAEKDKQLDQQQQLTAKAMADKETLKLELEEAKAEANQARLQVEEVQ
                     AEVGPKKGFLTRLFAK"
     gene            2742..3329
                     /locus_tag="VC122_001373"
     CDS             2742..3329
                     /locus_tag="VC122_001373"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002992267.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3'-5' exonuclease"
                     /protein_id="HEP5641294.1"
                     /translation="MKHLDTYIAFDLEFNTVNDVSHVIQVSAVKYDHHKEVDSFDTYV
                     YTDVPLQSFINGLTGITSDKIAAAPKVEEVMAAFKNFVGELPLIGYNAQKSDLPILAE
                     NGLDLRDQYQIDLFDEAYDRRSADLNGIANLRLQTVATFLGIKGRGHNSLEDARMTAA
                     IYKSFLETDTNKAYLSQQEEVTTDNPFAALGDFFD"
     gene            3482..4210
                     /locus_tag="VC122_001374"
     CDS             3482..4210
                     /locus_tag="VC122_001374"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002988202.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MerR family transcriptional regulator"
                     /protein_id="HEP5641295.1"
                     /translation="MSQFYSTGELANLAGVSIRTVQYYDQRGILIPTALTEGGRRLYT
                     DSDLEQLRMICFLRDLGFSIEQIRKVLAEENAAQVLELLLVDHIATAKEDLAAKEQQV
                     DIAVKILDRLRKQDPQSLDFLMDISLSMKNQKAWKKRFITLIVCLTVETLLFVGGAYF
                     LQLTVWKYLLIPVFLLVLLGTLYWYYQNICYLCPSCHQTFEAKWTEFALAGHTPRTRK
                     LTCSHCHQKSYCLELAKEDTVSRG"
     gene            complement(4267..4566)
                     /locus_tag="VC122_001375"
     CDS             complement(4267..4566)
                     /locus_tag="VC122_001375"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016503193.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YbaB/EbfC family nucleoid-associated protein"
                     /protein_id="HEP5641296.1"
                     /translation="MMNMQNMMKQAQKLQKQMEQKQADLAAMQFTGKSAQDLVTATFT
                     GDKKLVGIDFKEAVVDPEDVETLQDMTTQAINDALTQIDEATKKTLGAFAGKLPF"
     gene            complement(4759..5133)
                     /locus_tag="VC122_001376"
     CDS             complement(4759..5133)
                     /locus_tag="VC122_001376"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002988207.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HEP5641297.1"
                     /translation="MSAKKTFFASNLKYLRLKKNMEQLELANLLGRKSSSSISEWEKG
                     KYTPKSGLLSDIAAIFDVSLTALMEEDLTLESKGVSYQLPQRELEMISEQLSENNYTK
                     WVDFAQILLEQQAKEDRELDNH"
     gene            5303..5524
                     /locus_tag="VC122_001377"
     CDS             5303..5524
                     /locus_tag="VC122_001377"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002983026.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HEP5641298.1"
                     /translation="MTKSTLKELRTTKKMTQQELSHLTGISVRTIARYEKDTKKLRRA
                     KYEKLKGIAQALAVSVDDIFLGIDSEFMN"
     gene            complement(5604..7886)
                     /locus_tag="VC122_001378"
     CDS             complement(5604..7886)
                     /locus_tag="VC122_001378"
                     /EC_number="3.4.14.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014407825.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Xaa-Pro dipeptidyl-peptidase"
                     /protein_id="HEP5641299.1"
                     /translation="MRYNQFSYIPTSLERAAEELKELGFDLDLQKTAKANLESFLRKI
                     FFHYPDSDYPLSHLIAKNDMDALSFFQSEQELSKEVFDLLALQVLGFIPGVDFTEADA
                     FLDKLAFPIHFDETEIIKHIHHLLATRCKSGMTLIDDLVSQGMLTMDNDYHFFNGKSL
                     ATFDTSQLIREVVYVEAPLDTDQDGQLDLIKVNIIRPQSQKPLPTLMTPSPYHQGINE
                     VANDKKLYRMEKELVVKKRRQITVEDRDFIPLETQPCKLPIGQNLESFSYINSYSLND
                     YFLARGFANIYVSGVGTAGSTGFMTSGDYAQIESFKAVIDWLNGRATAYTSHSKTHQV
                     RADWANGLVCTTGKSYLGTMSTGLATTGVDGLAMIIAESAISSWYNYYRENGLVCSPG
                     GYPGEDLDVLTELTYSRNLLAGDYLRHNDHYQELLNQQSQALDRQSGDYNQFWHDRNY
                     LKNAHQIKCDVVYSHGLQDWNVKPRQVYEIFNALPSTINKHLFLHQGEHVYMHNWQSI
                     DFRESMNALLCQKLLGLANDFSLPEMIWQDNTCPQNWQERKVFGTSTIKELDLGQELL
                     LIDNHYGEDEFKAYGKDFRASKAALFKGKANQALIDILLEEDLPINGEIVLQLKVKSS
                     ENKGLLSAQILDYGKKKRLGDLPIALTQSSIDNGQNFSREPLKELPFREDSYRVISKG
                     FMNLQNRNNLSSIETIPNNKWMTVRLPLQPTIYHLEKGDTLRVILYTTDFEHTVRDNS
                     NYALTIDLSQSQLIVPIASN"
     gene            complement(7908..8567)
                     /locus_tag="VC122_001379"
     CDS             complement(7908..8567)
                     /locus_tag="VC122_001379"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017646447.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Crp/Fnr family transcriptional regulator"
                     /protein_id="HEP5641300.1"
                     /translation="MNHHILQRYIDNHNFPIIEKSYHKYLTFESLEEDFTYILKDGIV
                     KQSVLSKYGMEFNLRYVTGLEITSVLNTGYSKDMGEPYNVRIESEKASFYKVRRSAFL
                     KDINEDIELQGYVKDFYHNRLQKSMKKMQCMLTNGRIGAISTQIYDLMTLFGEELPNG
                     QILINFVITNEELGKFCGISTASSVSRILKQLKEKNIIRIDKQHIIITNLDKLKDNIV
                     F"
BASE COUNT         2868 a         1438 c         1658 g         2674 t
ORIGIN      
        1 tccagaagta aataaagaga tgatttatat caataaatcg ggcgctaata ttcaaaataa
       61 agtcaatgga acagactatc agctgttaga taataaggta gcaacaatct atattcctga
      121 taaatttaaa gagaaaaaga aatcaattga aaataccgtt gtggcagaac aatttactgg
      181 aactaagtat acaaaagaaa accttgcagt gcagataatt cctaatgaga aaaaaatttt
      241 ttattttaat gagaatggaa acgagcatga tgaaaataaa gaagcctcac ctatggcaaa
      301 tgtaacagat agtaaaggta atatagtcgt tgtgttagat actgataaaa tggtagcaag
      361 caaagatttt tctttggcta gcaatatagt aaatagctct ctttttagtc ctgaagcaat
      421 aaaaaaaatt aatgacctga gtctacattt aaatttttca attaatccag tagatgttta
      481 ccaacttgta aaacttaata ttcaatcctt aaagcaccaa attttccttt cgaaagtttt
      541 gcagaaaatt atttacggca ttgtatttct actaatttat caatatgctc aattatttat
      601 ttcttcaaaa cagaatgact atgtgaaaaa aataatttta ggcctctcaa aaacacgcat
      661 agcaatttcg agtctcaaat attttattat gactattgca atagttataa tatgtacatt
      721 tatggtgaca gagcagattg agttattata tattggtatt gcttcattag tggttttaat
      781 tctttcgaca gtaatgagtt ttagaaaatt atcaaaaaaa tacactcaaa ttttaaaagg
      841 agatgaacaa tgacaattga aattttcaac gttacaaaaa aattcagctc aaaaaaaata
      901 tttacagatt taaatttaac gtttgaagct ggaaaaagct atgccttgat tggaggttct
      961 ggctctggta aaagtactct gcttaacatt ataggaagac tagaaaaaat tgataacggg
     1021 aaagttttag ttgacgaaca agatatttgg aaaataaaag aaagaacttt ctttaaaaat
     1081 attatcgggt atgtattcca gaattattct ttaatagaaa acaaaactgt atatgataat
     1141 ttgaaactca tcaataaaga caaaaaaaat atctgctgta cttgaaaaag tagggctctc
     1201 tagtggctat ttaaatcaaa aaatatatga attgtctgga gggcaggctc agcgtgtagc
     1261 tattgcaaga atgttaatga agccacgtaa aataatttta gcagatgagc caacaggagc
     1321 tttggatggt gaaattggaa aagaaattat taatttgttg ttgagcgaaa cagccgaaaa
     1381 taaatacgtt attatagcaa ctcatgatcc agctgtatat aataaggttg attttataat
     1441 tgatatgaaa gatataggga gtaagaaatg aaaaagaaaa tttatattgg cttgattgtc
     1501 attgttggag tttttgtagt gttcttgtct ggaaaacaaa tcacacaaaa agaaacttca
     1561 aataagcctg tggtgaccct aacagtttat accatctcct ctagtgatac ccaaaaatgg
     1621 agtaaagtaa agcagtttga aacgaaagaa gcaatatacc tagtaaaggt aaaagaaact
     1681 acctcttcag aagaagtctt ttcaaacatc attgcagatg gaatggcagt tggttttgga
     1741 gttagtgaag aagacgttaa acagtttaac gataatttat gtagcccggt agaagatgct
     1801 aagaataata aattaattgg aatagaattc atcaattttt ctagtgttga taaggggttt
     1861 gtggttgcaa attttgacta cggcaaaaaa gcgtttaatt ctcagaaaac agaaaataaa
     1921 aaattatata aaaagctcta tgaagcgtat aaaggatagc tatttatagt tttttgagag
     1981 tgataccact tctgaataat aaatgaacta acatggcata ccgttttcta ttcatctaac
     2041 aatgttttca agaaataatt gatgccttta gcacgactga aatgaggtca aattgacaga
     2101 atactcctat tatggtacta tttttaataa gaatattcag tgatagtgat aaagaggaaa
     2161 atatgggaat tgaaaagaca gttagtgaac tagctgatat tttgggagtg agtcgtcagg
     2221 ccgtcaataa tcgtgttaag tctttacccg aagaagatct tgacaaaaat gaaaaaggcg
     2281 ttactgtggt taagcgcagt ggtcttgtta agctggaaga gatctataaa aaaacaattt
     2341 ttgacgatga gcctatcagt gaagaaacga aacaacgtga gcttttagag attcttgtgg
     2401 atgagaagaa cactgaaatc acgcgccttt atgagcaact caaagccaaa gatgctcaac
     2461 ttgcctcaaa agatgagcaa atgcgtgtta aagacgtgca gatcgctgaa aaagataaac
     2521 aattggatca gcagcaacaa ttaactgcta aagccatggc tgataaagaa accctaaaac
     2581 tagaattgga agaagctaaa gcagaagcca accaagctcg cttacaagtc gaagaagttc
     2641 aggctgaagt tggccctaaa aaaggctttt taacccgctt gtttgcgaaa taatcacaaa
     2701 aaatgacgaa ggccctatca gggtcttttt tggaggaaaa catgaaacac ttagatactt
     2761 atattgcctt tgatttagaa tttaacactg tcaatgacgt tagtcatgtc attcaagtat
     2821 cagcagtcaa atatgaccac cataaagaag ttgacagctt tgacacctat gtttacacag
     2881 atgttccctt acagagtttt atcaatggcc taacaggaat cacatctgac aagattgctg
     2941 cggcaccaaa agtagaggag gttatggctg cgtttaaaaa ttttgtggga gagttaccac
     3001 tgattggtta taatgctcaa aaatctgacc tgcctattct agctgaaaat ggcttagatt
     3061 taagggacca ataccagatt gacctctttg atgaagctta cgatagacgt agcgcagact
     3121 taaatgggat tgctaacctt cgtctgcaaa cagtggcaac ctttttaggc attaagggtc
     3181 gaggtcataa tagcttagaa gatgccagaa tgacagcagc aatctataag tcatttttag
     3241 aaacagatac aaacaaagca tatctcagcc agcaagaaga ggtaaccaca gataaccctt
     3301 tcgcagcttt gggagacttt tttgactaag ggctctgcta tttctaaaaa ataacaaaaa
     3361 ttcctagggt tagctcttgc ttgtaacgct aggtcatgtg ataagatagg ggcatatcaa
     3421 gagggagtcg agtgcacacg aatcgttttt gatcactaaa tgatagaaag aaggtgtgga
     3481 aatgtctcaa ttctattcga ctggagaatt ggccaactta gctggcgttt ctattcgaac
     3541 tgtccaatac tatgaccaac gtgggatttt gattccgaca gctttgacag aaggtggacg
     3601 gcgactctat acggattctg atttagaaca attacgcatg atttgctttc tgcgcgattt
     3661 gggattttcc attgagcaga ttcgcaaagt cttagcagag gaaaatgcag cccaggtgct
     3721 ggaattgctt ctagttgatc atattgcgac tgccaaggaa gacttggccg caaaagagca
     3781 acaagttgat atagctgtca agatacttga cagacttcga aaacaagacc cccagtcact
     3841 agattttctg atggacattt cgcttagcat gaaaaatcaa aaagcttgga aaaaacgctt
     3901 tattacttta attgtctgtt tgacagtgga aactctactt tttgtgggtg gagcctattt
     3961 tctccaattg acagtctgga aatatttatt aatcccggtg ttccttttag tcttactagg
     4021 aaccttatac tggtattacc aaaatatctg ttatctttgt ccttcttgtc atcaaacatt
     4081 tgaagcaaaa tggacagaat ttgcccttgc aggccataca ccacgaacca gaaagttaac
     4141 ctgctcccat tgccatcaaa aatcgtattg cttggaactg gcaaaagagg acactgtctc
     4201 aagaggataa gcactaactc ataaaaaaac tagcctgaga tccttctcaa gctagttttt
     4261 gtttttttag aatggtaatt taccagcaaa agcgcctaat gttttcttag tcgcttcatc
     4321 aatttgcgta agggcatcat taatggcttg agtggtcatg tcttgaagcg tttctacatc
     4381 ttcaggatct acaacagctt ctttgaagtc aatcccaacc aattttttat cacccgtgaa
     4441 agtggcagtg acaaggtctt gagctgattt tccagtaaat tgcatagcag ccaaatccgc
     4501 ctgcttttgc tccatttgtt tctgaagctt ttgtgcttgc ttcatcatgt tttgcatgtt
     4561 catcataaga ctatactctc cttaatttaa tactaactat tataacatat aaaaagaggc
     4621 aataaaaaca atgtcagtta gtagactagc ctgtatcagt aagaaagtcg ctatggataa
     4681 gatggattag cagcaagggt ctggctaatg acagaataag tctagaaatg gattattttt
     4741 gtcatagact agtccgactt agtggttatc taattcacga tcttctttag cttgttgttc
     4801 taaaagaatt tgtgcgaagt caacccactt agtgtagtta ttttcggata attgttctga
     4861 aatcatctct aactctcgtt gtggcaactg ataagagact cccttacttt ctaaggttaa
     4921 atcttcttcc attagggcgg ttaaactaac atcaaagata gcagcaatat cacttaatag
     4981 accagacttt ggagtatatt ttcctttttc ccactcactg attgaagaag agctttttcg
     5041 tcctaggaga ttagcaagtt ctaattgttc catgttcttt tttaatctaa ggtactttaa
     5101 attacttgca aaaaaagttt tcttggcgga catatttttc tccttttctt tatataaaat
     5161 atgataccat aaaagaggtt aaagcaaaag gtatcgtaaa agatggtcta aaaactctct
     5221 aaaaacttga cttcataaaa accgaagttt ataataaact taacatgatt aactaatctc
     5281 attttagtgt ggagggaaaa ttgtgaccaa atcaacttta aaagagttaa gaacaacaaa
     5341 gaaaatgaca cagcaggaat tgtctcactt aacaggcatt tctgtcagaa cgattgctag
     5401 gtatgaaaaa gatactaaaa agctaaggcg agcaaaatat gagaagctaa aaggtattgc
     5461 acaagcatta gcggtttccg ttgatgatat ttttttagga atagattcgg aatttatgaa
     5521 ctaagacgat cgttttctgt ttgctattag taagaccaat taacaaaaaa gtgcttagtt
     5581 atattgctaa gcacttttat ttgttaattt gatgctattg gcacaatcaa ttgagactga
     5641 ctgagatcga tagttaaagc atagttgctg ttgtcacgga cagtatgttc aaaatcggtt
     5701 gtgtataaga tgactcgaag ggtatctcct ttttctagat gatagatagt tggttgcaaa
     5761 ggaagtctaa cagtcatcca tttattatta ggaatagttt caattgacga caaattattg
     5821 cgattttgta aattcatgaa acctttcgaa ataacacggt aagaatcttc tctaaatggt
     5881 agctctttaa ggggttctct agagaaattt tgcccattgt caatacttga ttgggtgaga
     5941 gcaataggaa gatcgcccaa gcgttttttc ttaccatagt caaggatttg tgcagataga
     6001 agtcctttat tttcacttga tttcactttt aactgtagga caatttcacc gttaataggt
     6061 aagtcctctt ccagtaaaat atcaatcagg gcttgattgg ccttgccttt aaagagagct
     6121 gctttggacg cacggaaatc tttaccgtaa gctttaaatt catcttcacc atagtgatta
     6181 tcaattaaaa gcagttcctg accgaggtca agctctttga tggttgaggt tccaaagact
     6241 ttacgctcct gccagttttg tgggcaggtg ttatcctgcc agatcatttc aggaaggcta
     6301 aaatcattag ctaggcctaa gagtttttga cataaaaggg cattcatgct ttcgcgaaaa
     6361 tcaatggatt gccaattatg catgtaaaca tgctcacctt gatgtaaaaa gaggtgcttg
     6421 ttgattgtgg aaggaagcgc attgaaaatc tcatagacct gtcttggttt gacattccag
     6481 tcttgtaagc catgactata aacgacatca cactttattt gatgagcatt ttttaagtaa
     6541 ttgcggtcgt gccaaaattg attataatca ccagattgtc ggtctaacgc ctgagactgt
     6601 tggtttagca attcttgata atgatcatta tggcgaagat aatcgccagc caataaatta
     6661 cgcgagtagg tgagctcagt taggacatct agatcctctc caggatagcc accaggacta
     6721 caaactaagc cattttcgcg gtagtagttg taccaagaag aaatagctga ttctgcaata
     6781 atcatggcta atccgtctac gcctgtagtg gctaaaccag tagacatagt tcccaagtag
     6841 gactttccag tcgtacaaac tagaccattt gcccaatcag ctctcacttg gtgggttttt
     6901 gagtggctgg tgtaggctgt ggctcttcca tttagccaat caataacggc tttaaagctt
     6961 tcaatttgag catagtctcc actcgtcata aagccagtgg aaccagcagt tcccacacct
     7021 gaaacataga tattagcaaa cccacgtgcg aggaaataat cgttgaggct ataggagttg
     7081 atgtagctaa agctttctag attttgacct ataggaagtt tgcaaggctg tgtctcaaga
     7141 ggaataaaat ccctgtcttc gacagtgatt tgtctccttt ttttaacgac taactctttt
     7201 tccatacgat aaagtttttt gtcgttggct acttcattga taccttgatg gtatggacta
     7261 ggtgtcatca aagttggtaa tggtttttga gattgaggac gaatgatatt aaccttaata
     7321 agatcaagtt ggccatcttg atcggtatca agaggtgctt caacgtaaac aacttctcga
     7381 atcagctgtg acgtgtcaaa ggtagctaat gatttaccgt tgaagaaatg gtaatcattg
     7441 tccatagtaa gcattccttg gctgaccaaa tcatcaatca gggtcatgcc agacttacaa
     7501 cgggtcgcta ataagtggtg gatatgcttg ataatttctg tttcgtcaaa gtggatagga
     7561 aaggccagtt tatcaaggaa agcatcggcc tctgtaaaat caactccagg aataaagcca
     7621 aggacttgca gggccagtaa atcaaaaacc tctttggata actcttgctc tgattgaaaa
     7681 aagctcaatg cgtccatgtc atttttggcg attagatgac ttaaaggata atcactatca
     7741 gggtagtgga agaagatttt acgtaaaaag ctttctaggt tagctttggc agttttttgc
     7801 agatccagat caaagcccaa ttcttttaat tcttcagccg ctctttctag actagttgga
     7861 atataggaaa attgattata gcgcatagga gtttcctttc tagtggctta gaaaacgata
     7921 ttatctttta atttatcaag attggtaatg ataatatgtt gtttatcaat gcgaatgatg
     7981 tttttttctt tcaactgttt tagaatacgg ctgacgctgc tagcggtaga aatgccacag
     8041 aatttaccga gttcctcatt agtaatgaca aaattaatta aaatttgacc atttggtagt
     8101 tcttcgccaa atagggtcat taagtcatat atttgagtag aaatggcacc gatacggccg
     8161 ttagttaaca tacattgcat ttttttcatg gatttttgaa gacgattatg gtaaaagtcc
     8221 ttcacatatc cttgtagttc aatatcttcg tttatatctt ttaaaaatgc tgagcggcga
     8281 accttgtaaa aactagcttt ctctgactcg atgcgaacat tataaggctc tcccatatct
     8341 tttgaataac cagtgttgag aacagaagta atttcaagtc ctgtaacata tctcaaatta
     8401 aattccattc catattttga aagaacactc tgtttcacaa tgccgtcttt taaaatataa
     8461 gtgaagtcct cttcgagact ttcaaaagtc aagtacttgt gataactttt ttcaatgata
     8521 ggaaagttat gattatcaat ataccgttgc aaaatgtgat gattcacgct tttctccaaa
     8581 tattctttta ccaatgtata atttgtatct tcaagagaaa ttaacatttg ctaatgcc
//
LOCUS       DATXIG010000053         8493 bp    DNA     linear   BCT 28-DEC-2023
DEFINITION  TPA_asm: Streptococcus pyogenes strain MGAS11113
            SAMN08555799-rid19682583.denovo.053, whole genome shotgun sequence.
ACCESSION   DATXIG010000053 DATXIG010000000
VERSION     DATXIG010000053.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN08555799
            Sequence Read Archive: SRR6740495
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pyogenes
  ORGANISM  Streptococcus pyogenes
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 8493)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8493)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-OCT-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-OCT-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001918058.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001918058.2
            Genome Coverage       :: 368x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/12/2023 11:23:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 1,806
            CDSs (total)                      :: 1,769
            Genes (coding)                    :: 1,722
            CDSs (with protein)               :: 1,722
            Genes (RNA)                       :: 37
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1, 1 (5S, 23S)
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 47
            CDSs (without protein)            :: 47
            Pseudo Genes (ambiguous residues) :: 0 of 47
            Pseudo Genes (frameshifted)       :: 30 of 47
            Pseudo Genes (incomplete)         :: 20 of 47
            Pseudo Genes (internal stop)      :: 9 of 47
            Pseudo Genes (multiple problems)  :: 11 of 47
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8493
                     /organism="Streptococcus pyogenes"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN08555799-rid19682583.denovo.053"
                     /strain="MGAS11113"
                     /serotype="Emm28"
                     /isolation_source="normally sterile site"
                     /host="Homo sapiens"
                     /db_xref="taxon:1314"
                     /country="Finland"
                     /collection_date="2002"
                     /collected_by="National Public Health Laboratory"
     gene            83..265
                     /locus_tag="VC122_001380"
     CDS             83..265
                     /locus_tag="VC122_001380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003045758.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEP5641301.1"
                     /translation="MTSVRTAQLLNNGLGLVGQDLKDVLSALANSVNPSIEKQISHIM
                     IDRANTGEWLANVGKK"
     gene            277..609
                     /locus_tag="VC122_001381"
     CDS             277..609
                     /locus_tag="VC122_001381"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002992497.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEP5641302.1"
                     /translation="MKYRVETNPFSKDRYTPEQLEMFKNRQLSKNKAEAYFTRLYNQH
                     IAWVIIANVMTEYVNKFRKSATSFEKAWDALGYQQTTEIVFRAVNGLPCSEKDTGELE
                     TYLSEVSA"
     gene            609..800
                     /locus_tag="VC122_001382"
     CDS             609..800
                     /locus_tag="VC122_001382"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017645222.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEP5641303.1"
                     /translation="MQELNLTPTQTLILFIVLGLVGLLLSLSKPLIEIDLPEDTQVPK
                     PPQNANYGAYIQSQNHYYN"
     gene            709..1077
                     /locus_tag="VC122_001383"
     CDS             709..1077
                     /locus_tag="VC122_001383"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012767668.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEP5641304.1"
                     /translation="MTYQKIPKYLNHLRTQTMGLIFNHRTIITIRREQMQLLSREAEL
                     ELLEKVGCYLEKRWQLELENKDDWDLIPRQDLLEKLDITGTTLKNWEKEGLKSYQSPF
                     ENSKKVYYRKSDIYNFLSVD"
     gene            1087..1608
                     /locus_tag="VC122_001384"
     CDS             1087..1608
                     /locus_tag="VC122_001384"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011285314.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEP5641305.1"
                     /translation="MDYLEVTMTKDCVKIFNFLYKYPTKAYQKDEYFKLLYMHPLDSF
                     LTSFSLSGIRVKVTDKPVLAGWKLVRDIEVRIATGELLEMIEELEICYLRKHQTVSYV
                     EIKFYVVHLLTYGIRSRYDMQFFTKLLFCCGYDQETVIGIYSNITKNTRLSRDFITLQ
                     AKLYQTKKGTHEH"
     gene            1598..3211
                     /locus_tag="VC122_001385"
     CDS             1598..3211
                     /locus_tag="VC122_001385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011285313.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage resistance protein"
                     /protein_id="HEP5641306.1"
                     /translation="MSIKENIDKLVSMEWTESIFGQSKMKADYKRQLSKVIELDTLSN
                     IHHNGETYAVSNQEVMYLSESKGRTHKFISGNVKTKSKGKGRDSQKYLVVSFDHQFPP
                     FAKLVVDYLLGRFTFFNNKLYDVNNRQAQVLDDFTIQSLYGFKKDNPFILEILEGIHQ
                     TLNIRPAKQLEAYKISGRDFIIDLKEHRLYRTNPSSDSSFFKFYDVDYHTAIGSKKKV
                     GKFLDYVIADHDSLHNATLQAYYIAQVACGLKSKQNFFISKSGVRTGKGLRHIGLSGI
                     FNKIDVELDLLIKGGFEASNAWGAFAGGEMALATEQGDIVGDKMERVLKIIATEKTHM
                     ARSIGGNLGTVNLTSVLCIDTNRTVALSDEMNGRAVLIQYQNRPEGETDLEREKAFEE
                     YWQTFTEMDKSPKIEGVIGFLLVSLDYFRKNDMKFDWRAVEVVNDDDLDDFQVYLINH
                     LSEKDFVERDSYVRELYNSTYGKNNNQAGQRMRTIGVKSYRKQTTGGRKIPGYKVDNI
                     KRFNKFSGGEKENTNIFDMFEKLNQPLPP"
     gene            3498..3671
                     /locus_tag="VC122_001386"
     CDS             3498..3671
                     /locus_tag="VC122_001386"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000694572.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2758 domain-containing protein"
                     /protein_id="HEP5641307.1"
                     /translation="MKIKLFYQKHNESLDDFEYRVNQFTLSVSVIDIKLSEATYGNYE
                     DMSTTTSLLVLYR"
     gene            3677..3850
                     /locus_tag="VC122_001387"
     CDS             3677..3850
                     /locus_tag="VC122_001387"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001873750.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEP5641308.1"
                     /translation="MKLKLHTRGGNTITIQGDSAFYDELVKYLLSGRQPNWVTCPSAV
                     INLSDIIAITKEK"
     gene            3852..4178
                     /locus_tag="VC122_001388"
                     /pseudo
     CDS             3852..4178
                     /locus_tag="VC122_001388"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001258765.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            4174..4419
                     /locus_tag="VC122_001389"
     CDS             4174..4419
                     /locus_tag="VC122_001389"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011285310.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEP5641309.1"
                     /translation="MSNQMAVFNQFWAYQTLFQPTAQDIKLAILGYFTGTLWACEVSV
                     LEDVELGYRVIVSYVSCFDKSDRLLKIAQAVENSQLG"
     gene            4433..4591
                     /locus_tag="VC122_001390"
     CDS             4433..4591
                     /locus_tag="VC122_001390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015058151.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEP5641310.1"
                     /translation="MQTEVKDGFIYFKENGRIGVVEIPTFGKLTLSSKEGYIHQVDHS
                     FSTKHNQK"
     gene            4682..5470
                     /locus_tag="VC122_001391"
     CDS             4682..5470
                     /locus_tag="VC122_001391"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011285308.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEP5641311.1"
                     /translation="MYKQTNKTYADILEKVEAATAQWIIRKKASSLKDDVIITDEDIQ
                     AEKERIFIEKYGTALFDFKIDSYRKELDDLKIKAREQVNQFNDFVKELEQDDRYTERG
                     KVDLYRAERKKAEKTLKEIGEAQHKLNVDIQKIEVESAQNAWKKLESEMTPDSITPSE
                     YQYLEMMLSRNDSDEMRKKLAEKFHYHLMVLEYLNADRKFGEVIIKHPLEGVKNQSVN
                     YIKIGDVRLPEVYESTYIHELLKHYKNDIYFGESYANHEWDEEQ"
     gene            5467..5733
                     /locus_tag="VC122_001392"
     CDS             5467..5733
                     /locus_tag="VC122_001392"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011285307.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEP5641312.1"
                     /translation="MMSLECLRIYLKKSQFTELEHLLFRIIVLGGYPDDMYFPSRVRT
                     IITSLVNNIRKNLDSEGYRSVEELEEAIEKAISEHDEITQKGGG"
     gene            5775..6113
                     /locus_tag="VC122_001393"
     CDS             5775..6113
                     /locus_tag="VC122_001393"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012767659.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication protein"
                     /protein_id="HEP5641313.1"
                     /translation="MVAMVTSKTITEAAERAGITRGTAHRYLKDITFKKACRRYRSEL
                     MQQTTSLLQSASIEAVEVLRNIMLDDSISPYARQQSAQTILSTAYKAHELDDIVEMVE
                     ELEARFEDEQ"
     gene            6103..6378
                     /locus_tag="VC122_001394"
     CDS             6103..6378
                     /locus_tag="VC122_001394"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014407964.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEP5641314.1"
                     /translation="MNSNLKKRVDKLRRLHSLDNASGIVFVEYADGKYQIKKQDHVPK
                     FIETRLIAEFESEEEAENFLSTIPSGYIIFWDIFPEEVEHVKKNKYL"
     gene            6353..6598
                     /locus_tag="VC122_001395"
     CDS             6353..6598
                     /locus_tag="VC122_001395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011285304.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEP5641315.1"
                     /translation="MLKKINTYRRLEIIKNKLDEASKDSIEDIMLDWFRALHYALKVD
                     DTIDLPTFLEFKDNKALRDKIMADLGDFDPFEDLNIE"
     gene            6879..7226
                     /locus_tag="VC122_001396"
     CDS             6879..7226
                     /locus_tag="VC122_001396"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011889234.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1492 domain-containing protein"
                     /protein_id="HEP5641316.1"
                     /translation="MNAKEQLKELKPLFALMTLFEEQRDKDIKLMNAFRNPELLNGIE
                     KGTAKQLLYLAKERDKRLAMIATLQDERQIAVIKARYVDDLSWDEIPDKLGYSRNTVF
                     KLHREALEVLDEP"
     gene            7216..7587
                     /locus_tag="VC122_001397"
     CDS             7216..7587
                     /locus_tag="VC122_001397"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011285302.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1492 domain-containing protein"
                     /protein_id="HEP5641317.1"
                     /translation="MNHKNAIRKLKEFHRWQRIANSLNLTYNECYQFDIEYYSFRRKH
                     LEISRKCALEELDAIKHAINQLSKIEYRKILIECYLIGEKKPQQDIIAELNRSKSWYY
                     ETKRRALLEFVEFYRDGVLKK"
     gene            7740..7967
                     /locus_tag="VC122_001398"
     CDS             7740..7967
                     /locus_tag="VC122_001398"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002938604.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="translation repressor RelB"
                     /protein_id="HEP5641318.1"
                     /translation="MAMVSLRLNSAEEELFRSYATHTGKTLSELFKTALAQQIEDQLD
                     YETGIQALKRFEENPITYSIDDLIEELENDL"
     gene            7957..8226
                     /locus_tag="VC122_001399"
     CDS             7957..8226
                     /locus_tag="VC122_001399"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014407961.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelE/ParE family
                     toxin"
                     /protein_id="HEP5641319.1"
                     /translation="MTYKLVVSDEVKKQLKKMDKHVGLMLAKDMKKRLDGLNNPRQFG
                     KALTGQYKGLWRYRVGNYRVICDIVDNEMIILALEVGHRKEIYKR"
BASE COUNT         3005 a         1383 c         1719 g         2386 t
ORIGIN      
        1 gagctacccc ttaaaactag catgaatcta gtataagaaa caagcaacta cacagaaaat
       61 aagaattaat aaggaagata aaatgacatc agttagaacc gcacaattac ttaacaatgg
      121 tttaggattg gttggtcaag acctaaaaga cgtcttatca gctctagcca atagcgtcaa
      181 tccaagtatt gaaaaacaga tttctcacat catgatagat agagctaata ctggcgaatg
      241 gttggcaaat gtaggcaaaa aatgaggtaa gacataatga aatatagagt agaaacaaat
      301 cctttttcaa aagatagata cactcctgaa cagctagaaa tgttcaaaaa tcgacaactc
      361 agcaaaaata aagcagaagc ctatttcact cgactatata accaacatat tgcttgggtg
      421 attattgcta acgttatgac agaatacgtc aataaattca gaaaaagtgc caccagcttt
      481 gaaaaagcat gggacgcttt aggctatcaa caaaccacag agattgtctt tagagccgtt
      541 aacggtttac cttgttcaga gaaagacaca ggggaactag aaacttattt aagtgaggta
      601 tcggcatgat gcaagaactt aacctcacac caacacagac acttatttta ttcattgttt
      661 tagggcttgt tgggcttctt cttagccttt ctaagccatt aatagagatt gacttaccag
      721 aagataccca agtacctaaa ccacctcaga acgcaaacta tggggcttat attcaatcac
      781 agaaccatta ttacaattag gagggaacag atgcaattat tatcacgaga agcagaatta
      841 gagctactag agaaggtggg ctgttacctt gaaaaacgtt ggcaacttga actagaaaac
      901 aaagatgatt gggatttaat tccaaggcaa gatctactgg aaaaactaga cattactggt
      961 accacgttaa aaaattggga aaaagagggc ttaaagtcgt atcagtcgcc attcgagaac
     1021 agtaagaagg tgtattaccg taaaagtgat atttataatt ttttgagcgt tgactaggag
     1081 gactttgtgg attatttaga ggtcaccatg acaaaagatt gcgtaaaaat attcaatttt
     1141 ttgtataaat atcctaccaa agcataccaa aaggacgagt atttcaaatt actgtatatg
     1201 catcccttgg acagcttttt aacaagtttc tccctatcgg gcatccgagt gaaagttaca
     1261 gacaaacctg ttttagcagg gtggaaactt gttagggata tagaggttag aattgctacg
     1321 ggggaactct tggaaatgat tgaagagcta gaaatatgct atttaaggaa gcaccagacg
     1381 gtttcttacg ttgaaattaa gttctatgtc gtccatctat tgacttatgg tattaggtca
     1441 cgttacgata tgcaattttt taccaagttg ttgttttgtt gtggttatga tcaagaaact
     1501 gttatcggta tttatagcaa tataaccaaa aataccagac tttcaaggga ttttataact
     1561 ttacaggcta aactttacca aacaaagaaa ggaacccatg agcattaaag aaaacattga
     1621 caaattagta tctatggaat ggactgaatc aatttttggg caatctaaaa tgaaagcaga
     1681 ctataaacgc caactatcta aggtgattga gttggatacc ttaagcaata tacaccataa
     1741 tggggaaacc tatgctgttt caaatcaaga agtcatgtac ttaagtgaga gtaaaggtag
     1801 aactcataag tttatttcag gaaacgttaa aacaaaatct aaaggaaagg gaagagatag
     1861 ccaaaagtat ctcgtggttt cctttgacca tcaattccca ccttttgcta agttagtggt
     1921 tgattatcta cttggacgat ttactttttt taataacaaa ttatatgatg ttaacaatcg
     1981 tcaagcgcaa gtgttagatg atttcactat tcaatcgtta tacggcttca aaaaggataa
     2041 cccgttcatt ttagagattc tggaaggaat ccaccaaacc ttgaatatta gacctgcgaa
     2101 acaacttgaa gcttataaga tttctgggag agatttcatt attgatctta aggaacatcg
     2161 gttataccga acaaatccca gttccgatag ttctttcttt aagttttatg atgtcgatta
     2221 tcacacagcg atcggtagta agaaaaaagt tggcaagttt ttagattatg ttatcgcaga
     2281 ccatgatagc ttgcacaatg caacgttaca agcctattat atcgcacagg tcgcttgtgg
     2341 actgaaatcg aaacaaaatt tctttatctc taaatctggt gtgagaactg gtaaaggctt
     2401 aagacatatt gggcttagcg gtatctttaa taaaatcgat gttgagttag acttattgat
     2461 taaaggtggc tttgaagcta gcaatgcttg gggtgctttt gctggtggag aaatggctct
     2521 agcaacagaa caaggggata ttgtaggcga taaaatggag cgtgtgctta aaattattgc
     2581 tactgagaag acccatatgg ctagaagtat cggtggcaat ttaggaacag ttaatttaac
     2641 aagtgttctg tgtattgaca caaaccgcac tgttgcgctg tcggatgaaa tgaatggtag
     2701 ggcggttttg atccaatacc agaatagacc agaaggggaa actgatttag aacgtgaaaa
     2761 ggcctttgaa gaatattggc aaaccttcac tgaaatggat aaaagcccta aaattgaagg
     2821 agtgataggt tttttacttg tatcacttga ttacttccga aaaaatgaca tgaaatttga
     2881 ttggagagcg gtcgaagtgg tgaacgatga tgatttagat gattttcaag tttacctgat
     2941 taaccatctt tctgaaaaag actttgtaga gcgtgactct tatgttagag agctgtataa
     3001 ctctacttac gggaaaaata acaaccaagc cggccaaaga atgcggacga taggggttaa
     3061 gagttatcgc aagcaaacaa ctggaggacg caaaataccc ggctataaag tcgataatat
     3121 aaaacgattt aacaaattct caggaggaga aaaagaaaat actaatatct ttgatatgtt
     3181 tgaaaagcta aaccaaccac taccgccatg acccagacag taggggttgt ggggggtata
     3241 aacccagtgg tatcaaggga tagagtacaa tcacccataa ccacctataa ctatataggg
     3301 gagagagaat tattttttta gtagatatgt tatttcttta cattattttt attttttctc
     3361 ccttatctat atagaaaagt ggcggtaggt ggtggttgtg ctggcaaatg gctcaaccat
     3421 gcggttttaa gcacaccacc actttattta gggttgtgtc tacacccacc actttgagcc
     3481 aaaacaggag aaaaaacatg aaaatcaaac tattttatca aaaacacaat gaatctttag
     3541 atgattttga atatcgggtc aatcaattta ccctatcagt atctgtaata gatatcaaac
     3601 tttcagaagc aacttatggc aattatgaag acatgagtac cacaacttct ttactggtct
     3661 tgtacaggta actgatatga aactaaaatt acacacacga ggcggcaaca ccatcaccat
     3721 acaaggagac agtgcctttt atgatgagtt ggtcaaatac cttctttctg gccgacaacc
     3781 aaactgggta acatgtcctt ctgccgtcat caatttatca gacattatag caatcacaaa
     3841 ggagaaataa tatgagaaca ttttcagaca caccaaaaac attcacattt cactacacgt
     3901 ttaaagactt tgacaccgca caagtagcct gtcacgccat tctaggctat atgactggta
     3961 cttatgagca accagtgatt gatgccacct atcacaatga taatcaaggc gggcatgcta
     4021 atcagttagt attagaatat gccgaggaca gaaagttaag caaggtcttc aagcgtatct
     4081 gtgacagctt caaggattat tacaaccaac ctgaggatat gacggatgaa gaacttgatg
     4141 acatggttca agaaaatgaa ttaatcaagg aagttgagca accagatggc agtatttaat
     4201 caattttggg cgtatcagac actttttcaa ccaacggcac aagatattaa gttagctatt
     4261 ttaggatact tcacaggcac gctatgggct tgtgaggtgt ccgtgttgga agatgttgaa
     4321 cttggctacc gtgttatcgt ctcttacgtt agttgttttg ataaaagcga tagactgctg
     4381 aaaattgctc aagcagttga gaatagtcag ttaggatgaa aggaggtatt taatgcagac
     4441 agaagtgaaa gatggcttta tttatttcaa agaaaatggt agaataggag tagtagagat
     4501 acctactttt ggtaagctaa ccctatctag caaggaaggc tatattcatc aagtagacca
     4561 cagcttttct actaaacaca atcaaaaata attatactga ccgaagaacg gaggtatatt
     4621 catgaagctt aatggctttt ttggatatac ctctttttgc atttaagaaa ggaaagacac
     4681 gatgtacaaa caaactaaca aaacttacgc cgatattttg gaaaaagttg aagccgcaac
     4741 agcgcaatgg attattagaa aaaaagcttc aagccttaaa gatgacgtga ttatcactga
     4801 tgaggatata caagctgaga aagagcgtat tttcattgag aaatatggga cagcactgtt
     4861 tgattttaaa attgatagct accgtaaaga attagatgat ttaaaaatca aagctcgtga
     4921 gcaagtaaac caattcaatg attttgtaaa ggaactagaa caagacgacc gttacacaga
     4981 acgtggtaaa gtggatttat accgagcaga gcgaaaaaaa gctgaaaaga cccttaaaga
     5041 gattggagaa gctcagcata aacttaatgt tgatattcaa aagattgaag tagaatcggc
     5101 tcaaaatgct tggaaaaagt tagaatctga aatgacgccc gattcaatca cccctagtga
     5161 atatcagtat ttagaaatga tgttatcacg taatgatagt gatgaaatgc ggaaaaaact
     5221 tgctgagaag ttccattacc atctgatggt cttggagtat ttgaatgctg accgtaaatt
     5281 tggagaagtt atcatcaagc acccattaga aggggttaaa aatcagtcag ttaattatat
     5341 taaaattggg gatgtccgat tgcctgaggt ttacgaaagc acttatattc atgaactttt
     5401 gaagcattac aagaatgaca tttactttgg cgagagctac gcaaatcatg aatgggatga
     5461 agagcaatga tgtcattgga atgtttaaga atatacctta agaaaagcca gtttacagag
     5521 cttgaacact tgctattcag aattatagtg ttaggggggt atcctgatga catgtatttc
     5581 ccctcaaggg ttcgtacgat catcacaagc ttggttaata atatacgtaa gaatttagat
     5641 tctgagggtt accgtagtgt cgaagaattg gaagaagcta tagaaaaggc tatttcagag
     5701 catgacgaaa tcacacaaaa aggagggggg tgatttttat agaacatggt ttaacaccta
     5761 aacaagagaa attcatggta gcaatggtga ccagtaaaac tatcaccgag gcagccgaaa
     5821 gagcaggtat cacaagagga actgcccata gataccttaa agacattact ttcaaaaagg
     5881 cttgtcgccg ttatcgtagt gagttaatgc agcaaaccac atccttatta cagagtgcca
     5941 gcattgaagc cgttgaggtg ctacgtaaca ttatgttgga tgattctatt agtccatatg
     6001 caaggcaaca atcagcccaa actatcttaa gcactgctta taaagcgcat gaactagatg
     6061 atattgttga aatggttgag gaactagaag cgagatttga agatgaacag taacttaaaa
     6121 aagcgagttg ataagttaag gcgactacat agtttagata acgcttctgg tattgtcttt
     6181 gtagaatacg cagatggcaa gtatcagatt aaaaaacaag atcatgtccc taaattcatt
     6241 gagactagac ttatagcaga atttgaaagc gaagaagaag cagaaaactt tttaagcact
     6301 attccaagtg gttacatcat tttttgggat attttccccg aagaggttga acatgttaaa
     6361 aaaaataaat acttatagaa ggctcgaaat catcaagaat aagctcgatg aagcgagtaa
     6421 agatagtatt gaagatataa tgctggactg gtttagggct ttgcattatg ctcttaaagt
     6481 agatgataca attgatttac caacgttcct tgaatttaag gacaacaagg cattgagaga
     6541 taaaataatg gcagaccttg gagattttga cccatttgaa gatctaaata tagaatagtt
     6601 aaatggtgac agcatcgcca caaggtcgtc ataaggagat agttttttga ctgtctccct
     6661 ttttcgtcat ctattcccca tcagactaaa ataaaacatg gttttagcgc ttctgtctgc
     6721 taagttgatg attttatatt ataagctctg aaaagcgctt agaaacgatt ttagagccgt
     6781 tcttccgcta tcaagaatga ctaaaacaaa aagacgtcca cacggaacgc cttcatgatt
     6841 atatctgtta acacaattat accacactgg gggctttcat gaacgccaaa gaacaactta
     6901 aagaattgaa accacttttt gctttaatga ccttatttga ggaacaacga gacaaggaca
     6961 tcaagctaat gaatgctttt cgtaatcctg agttactaaa tggcatcgaa aaaggtacag
     7021 ctaaacaact tttatattta gctaaggaac gtgataagag actagccatg attgccacat
     7081 tgcaagatga gagacagatt gccgttatca aagctagata cgtggatgac ttatcatggg
     7141 atgagatacc tgataaacta ggttattcaa ggaataccgt ttttaaatta catagagaag
     7201 ctttagaggt attagatgaa ccataagaac gctattcgta aactaaaaga gtttcataga
     7261 tggcaacgta tcgctaatag ccttaattta acctataacg agtgttacca gtttgatata
     7321 gaatattatt cctttagacg taaacacctt gaaataagcc gaaaatgcgc cctagaggag
     7381 ctagatgcta ttaagcatgc cattaatcaa ctatctaaga tagagtatag aaagatactg
     7441 attgagtgtt acttgatcgg tgagaaaaaa cctcaacaag acatcatagc agaacttaac
     7501 agaagtaaaa gctggtatta tgagactaag agacgtgctt tacttgaatt tgtggaattt
     7561 tacagagacg gtgtgctaaa aaaataatgt tcgtttatga taacagagca atagatataa
     7621 atgttaataa tacagtgtta gaagagctta acaaggtata tcaatacggg taaataattg
     7681 actgattcct ccttatgtta tacaatgtat tacagataaa caaacagagg tggaagaata
     7741 tggcaatggt atcattaaga ctgaacagcg cagaggaaga acttttcaga agctacgcta
     7801 cacacacagg caaaacatta tctgaattat tcaagacagc actagcacaa caaattgaag
     7861 atcaattgga ctatgaaaca ggtattcaag ctttaaaacg ctttgaagaa aatcccataa
     7921 cttattctat cgatgattta attgaggagt tggaaaatga cttataagct agtggtgagt
     7981 gatgaagtca agaagcaact caaaaagatg gataaacatg tagggcttat gttagccaaa
     8041 gacatgaaga aacgacttga cgggttaaat aatcctagac aatttggaaa agccctcaca
     8101 ggtcaatata aaggactttg gcgctatcgt gttggtaatt atcgggtgat ttgtgacatt
     8161 gtggataatg aaatgattat ccttgctttg gaagttggac acaggaaaga gatatacaag
     8221 cgctagcaaa tacaagacat ggtacgaggt ataataaaaa gcgcagctaa tgaccacgct
     8281 atttcttgct tactgaactc attattttaa ttccttttta gttacccttt agatttctga
     8341 tagtgtatct agtttacact gtttttagag aaataagagt gataactcac ttggtttaca
     8401 gctactttac actactaaat atgagtagta taacaaaatg ctttaatttt aagattaaac
     8461 ttacaaaaag cctatttaat aggctttttt tgt
//
LOCUS       DATXIG010000054         8281 bp    DNA     linear   BCT 28-DEC-2023
DEFINITION  TPA_asm: Streptococcus pyogenes strain MGAS11113
            SAMN08555799-rid19682583.denovo.054, whole genome shotgun sequence.
ACCESSION   DATXIG010000054 DATXIG010000000
VERSION     DATXIG010000054.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN08555799
            Sequence Read Archive: SRR6740495
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pyogenes
  ORGANISM  Streptococcus pyogenes
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 8281)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8281)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-OCT-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-OCT-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001918058.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001918058.2
            Genome Coverage       :: 368x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/12/2023 11:23:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 1,806
            CDSs (total)                      :: 1,769
            Genes (coding)                    :: 1,722
            CDSs (with protein)               :: 1,722
            Genes (RNA)                       :: 37
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1, 1 (5S, 23S)
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 47
            CDSs (without protein)            :: 47
            Pseudo Genes (ambiguous residues) :: 0 of 47
            Pseudo Genes (frameshifted)       :: 30 of 47
            Pseudo Genes (incomplete)         :: 20 of 47
            Pseudo Genes (internal stop)      :: 9 of 47
            Pseudo Genes (multiple problems)  :: 11 of 47
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8281
                     /organism="Streptococcus pyogenes"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN08555799-rid19682583.denovo.054"
                     /strain="MGAS11113"
                     /serotype="Emm28"
                     /isolation_source="normally sterile site"
                     /host="Homo sapiens"
                     /db_xref="taxon:1314"
                     /country="Finland"
                     /collection_date="2002"
                     /collected_by="National Public Health Laboratory"
     gene            <1..615
                     /locus_tag="VC122_001400"
     CDS             <1..615
                     /locus_tag="VC122_001400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611423.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS3 family transposase"
                     /protein_id="HEP5641320.1"
                     /translation="VQRLMKVMSLAARIRRKRKYSSYKGEVGKKADNLIKRHFEGSKP
                     YEKCYTDVTELALPEGKLYLLPVLDGYNSEIIDFTLSRSPNLKQVQTMLEKTFPADSY
                     SGTILHSDQGWQYQHQSYHDFLESKGILPSTSRKGNSPDNGMMDSFFGILKSEMFYGL
                     ETTYQSLDKLEEAITDYIFCYNNKRIKAKLKGFSPVQYRTKSFQ"
     gene            complement(664..1395)
                     /locus_tag="VC122_001401"
     CDS             complement(664..1395)
                     /locus_tag="VC122_001401"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012560779.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rRNA pseudouridine synthase"
                     /protein_id="HEP5641321.1"
                     /translation="MRLDKFLVATGVGTRSQVKLLLKKKAIFVNQKVETSAKAHIDEY
                     KDLVTYQGTPLVYESFVYYLLNKPSGYVSATQDRQQATVMELLDDTARQKAVFPVGRL
                     DKNTRGLLLLTNNGQLAHDLLSPKKHVTKEYLAKVVGIMTEADKDYFARGISLKDHQC
                     LPAHLEVLASDLQQRTSLVKITIQEGKFHQVKRMVAACGKGVLDLQRLSMGPLKLDPS
                     LAEGEFRRLTPEELQSLAPYCQSSL"
     gene            1510..1875
                     /locus_tag="VC122_001402"
     CDS             1510..1875
                     /locus_tag="VC122_001402"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002984124.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioesterase family protein"
                     /protein_id="HEP5641322.1"
                     /translation="MTIYSHTYETRPNHSAKAMGSGTLNVLATPALVAFMENASYLFA
                     QEQLDSDFTTVGSEIAIQHLAASAIGDPVTIIITALKEEGRKYDFRLEAFVGEQLIGK
                     ACHTRVRVNSKHFMAKVAE"
     gene            complement(1995..3215)
                     /locus_tag="VC122_001403"
     CDS             complement(1995..3215)
                     /locus_tag="VC122_001403"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011017917.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HEP5641323.1"
                     /translation="MSTLSLDTTNKRALVAAIAASGTDDLNVMFLAFSMSSIMTDLSL
                     SGTQGGWIATITNLGMLVGGLLFGLLADRHHKFKVFKWTILLFSVATGLIYFTQSLPY
                     LYLMRFIAGIGVGGEYGVAIAIMAGIVPPEKMGRMSSLNGIAGQLGSISSALLAGWLA
                     PSLGWRGLFLFGLLPILLVIWMTLAIDDQKIWDHYGQEEEECSQPIKINELFKTKSLT
                     AQTLALMVMTTVQIAGYFGMMNWLPTIIQTSLNLSVKSSSLWMVATIVGMCLGMLCFG
                     QLLDCFGPRLIYSLFLLASSICVYLFQFANSMASMVIGGAIVGFFVNGMFAGYGAMIT
                     RLYPHHIRSTANNVILNVGRALGGFSSVAIGSILDASGISMVMIFLASLYVISFGAMW
                     SIGQLKAERYQQLR"
     gene            3589..5073
                     /locus_tag="VC122_001404"
     CDS             3589..5073
                     /locus_tag="VC122_001404"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008090588.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1846 domain-containing protein"
                     /protein_id="HEP5641324.1"
                     /translation="MKTIAFDSNKYLNLQRDHILERISQFDGKLYMEFGGKMLEDYHA
                     ARVLPGYEPDNKIKLLKELKEQVEIVIAINANNIEHSKARGDLGISYDQEVFRLIDKF
                     NTLDIYVGSVVITQYNNQPAADAFRKQLKKNGIASYLHYPIKSYPTDINHIISSEGMG
                     KNDYIKTSRNLIVVTAPGPGSGKLATCISQMYHDQINGVKSGYAKFETFPVWNLPLHH
                     PVNLAYEAATADLDDVNMIDPFHLETYGKTAVNYNRDIEVFPVLNRTFERILSKSPYA
                     SPTDMGVNMVGFSIVNEEAAIEASKQEIIRRYYQTLVDFKAERVTESAVKKIELLMND
                     IGVTPDDRHVTVAAHQKAEQTGQPALALQLPNGQIVTGKTSELFGPTAAVIINAIKTL
                     AKIDKTTHLIEPEYVKPIQGLKVNHLGSHNPRLHSNEILIALAITAMTSEEANLAMKE
                     LGNLKGSEAHSTVILTEEDKNVLRKLGVNITFDPVYQHHKLYRK"
     gene            complement(5178..5579)
                     /locus_tag="VC122_001405"
     CDS             complement(5178..5579)
                     /locus_tag="VC122_001405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002984115.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PaaI family thioesterase"
                     /protein_id="HEP5641325.1"
                     /translation="MSDLTQEMTLNVISIFDNYQIELAEKGHLILSTEVTETALNYYG
                     NAHGGYLFTLCDQVGGLVARTTGVESVTLQANANYLKAGHKGDKLMVEGRLVHGGRTT
                     QVVDVTIHNQTGALLTKTSLTMFVTGRRHNG"
     gene            complement(5664..6445)
                     /locus_tag="VC122_001406"
                     /pseudo
     CDS             complement(5664..6445)
                     /locus_tag="VC122_001406"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003060407.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside phosphorylase"
     gene            complement(6597..8276)
                     /locus_tag="VC122_001407"
     CDS             complement(6597..8276)
                     /locus_tag="VC122_001407"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000031864.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="HEP5641326.1"
                     /translation="MSKEKIKVYLYTRVSTSIQIEGYSLEAQKSRMKAFAIYNDYEIV
                     GEYEDAGKSGKSIEGRKQFNRMMEDIKSGKDGVSFVLVFKLSRFARNAADVLSTLQIM
                     QDYGVNLICVEDGIDSSKDAGKLMISVLSAVAEIERENIRIQTMEGRIQKAREGKWNG
                     GFAPYGYKLEDGKLFINEEEAVAIRTIFDQYVNTTIGANGLSKYLENHGIRKIPRQNG
                     KNPLFDAGLIRKILKNPVYNGKIAFGRRTLEKVHGTRNEYKQVEQDEYLISEGIHEAI
                     VSDEVWQAAQVKLKSQAKKYEHVNKGKDTRTHLLSGIVKCPICGAGMFGNKCTKKKKD
                     GTKYKDFYYYGCKHRQMIRGHKCTFRKQIREELLDDAVAEVIVKIVSNPKFASMMQEK
                     INMKVDTSEIEKEIDNYQKELRKSHSTKFKIIEEIDNLDVEDKHYKRRKQDLDDRLYR
                     MYDKIDELESSLIDAKAKKQTIEAEKLTGDNIYKVLIYFDKLYKVMNDVERRQLITAL
                     ISEIQVYEEKQPNGQWLKSITFKLPIIDDDLNISLDNNEQVECVSLLEKRS"
BASE COUNT         2509 a         1820 c         1435 g         2517 t
ORIGIN      
        1 gttcaacgtc tcatgaaagt catgagccta gcagcccgca ttcgtcggaa gcgcaagtat
       61 tcttcttaca aaggagaggt tggtaagaag gctgataatc tgattaaacg tcacttcgaa
      121 ggctctaaac cttacgaaaa atgttacact gatgtgacgg agttggcctt acctgaagga
      181 aaactctact tattacctgt tcttgacggc tataacagtg agattattga tttcactctg
      241 tctcggtctc ctaacttgaa acaggttcaa accatgcttg agaagacttt tccagcggat
      301 tcatacagcg gaactattct tcatagtgac caggggtggc aataccaaca ccagtcctac
      361 catgattttt tagagtctaa aggtattttg ccatccacgt cccgcaaagg gaatagcccg
      421 gataacggca tgatggattc cttctttggc attctcaaat ctgaaatgtt ttatggactc
      481 gagacaactt atcaatcact tgataagctt gaagaagcta ttacagatta cattttttgc
      541 tacaacaaca aacgaattaa agcaaaacta aaaggattta gtcctgtcca atacagaact
      601 aaatcctttc aataattatt tgtccaactt tttggggtca gtactgacag gggaggtttc
      661 tttttatagt gagctctgac aataaggtgc caggctttgc aattcctcag gcgtcaggcg
      721 cctaaactcc ccttctgcaa ggctagggtc tagttttaat ggtcccatgc tgagacgctg
      781 caagtcaagc actccctttc cacaggctgc taccatgcgt ttcacctgat ggaattttcc
      841 ttcttgtatg gtaattttaa ccaaacttgt tcgctgctgt aaatcactag caagcacttc
      901 taaatgagca ggtaggcact ggtgatcctt taaggaaata cctctagcaa aataatcttt
      961 atcagcttca gtcatgatgc ctactacttt agccaagtac tctttcgtaa catgtttttt
     1021 aggggataaa aggtcatggg ctagttgtcc attattggtc aagagcaaaa gtccacgggt
     1081 attcttatct aacctaccca caggaaaaac tgctttttgc ctagccgtgt catctaacaa
     1141 ctccataaca gtagcctgct gtctgtcctg ggttgctgac acataccctg acggcttgtt
     1201 taacaggtaa taaacaaagg attcatagac caagggcgtg ccttgatacg tcaccaaatc
     1261 cttgtattca tcaatgtgtg ctttggcaga ggtttcaacc ttttgattga cgaaaatagc
     1321 cttctttttt aagagaagtt tgacttggct cctagttcca acaccagttg ccactaaaaa
     1381 tttatctaag cgcatcataa ccctttcaaa gaaaattcat tattaagtat aacataaaaa
     1441 aacagtagtt tcatgactgt aagcgcttaa tatattataa taggcttata gaaacgagga
     1501 gattaactta tgactattta ttctcacact tatgaaaccc gtcccaatca ttcggctaaa
     1561 gccatgggct caggaacgct gaatgtccta gctactcctg ctcttgttgc ttttatggaa
     1621 aatgcttctt acttatttgc ccaagaacag ctcgacagtg actttacaac ggttggcagc
     1681 gagatagcta tccaacattt ggcagcttca gccattggag atcctgttac aatcatcatt
     1741 acagccctta aagaagaagg ccgaaaatac gatttccgct tggaagcttt tgtgggagaa
     1801 caactcattg gaaaagcctg tcatactaga gttagggtga acagtaaaca ttttatggcc
     1861 aaagtagcgg agtaaaacaa aagaaaagaa ccaaaaaggt aagcaatccc taagtgctta
     1921 cctttttggt tatctcaatc aataaaatag ctttgtgagt gaaagtaact caaatactaa
     1981 cacccatctc tttgctaacg caattgttgg tagcgttctg cttttaactg accaatagac
     2041 cacatagctc caaaactaat cacatacaga gaagctagga aaatcatgac cattgaaata
     2101 cctgaggcat ctaaaatact accaatagca acggacgaaa atcctcctag agcacggcca
     2161 acattcaaaa taacattatt agccgtggat cgaatgtgat ggggataaag tctggtaatc
     2221 atagccccgt agccagcaaa catcccattg acaaaaaaac cgacaatagc acctccaata
     2281 accatactcg ccatggaatt ggcaaattga aaaaggtaaa cacaaataga tgatgctaat
     2341 aaaaagaggg aataaatcag acgtggacca aagcaatcta aaagctgacc aaaacacagc
     2401 atccccaaac acatgccgac aatagtagcc accatccaca aggacgaact ttttactgaa
     2461 aggtttaagc ttgtttgaat aatggttggc aaccaattca tcattccaaa atagcccgca
     2521 atctgcacag tcgtcattac cattaaagca agggtttgag ctgttaagga ttttgttttg
     2581 aatagttcat tgattttaat tggttgactg cattcttcct cctcttgccc ataatgatcc
     2641 caaatttttt ggtcatctat ggctaaagtc atccaaatca ccaagagaat cggaagtaat
     2701 ccaaagagaa aaaggccacg ccagcctaga cttggagcca gccaccctgc taaaagagcc
     2761 gaactaatgg agccaagctg gccagcaata ccatttaaag aagacatccg acccattttt
     2821 tctggtggta caatccctgc cattatggca atggctacac cgtactcacc tccaactcct
     2881 atgccagcaa taaatcgcat caggtacagg tagggcaaag actgggtaaa ataaattaat
     2941 cctgttgcta ctgaaaacag taaaatcgtc catttaaaga ccttaaactt gtggtgccta
     3001 tcagccagca acccgaataa gaggcctccc accaacattc ctaaattggt aatggtcgca
     3061 atccagccac cctgagtgcc actaagactc aagtctgtca taatggaaga catggaaaag
     3121 gcaagaaaca tgacattcaa gtcatccgtt cctgaagctg caatagctgc cactaaagct
     3181 cgcttatttg tggtgtccaa tgataatgtt gacataattg tctccttctt aatagtagct
     3241 cactgactac cttaattaat tgaataccct ttgtccaaag gtcaaaaaaa gacttggaaa
     3301 caagtccgca agtccatcaa agttcactaa ataataagag atgacaaaaa aactctcttt
     3361 aatgttaaga ctttcatgct tcacggaaca tgtttaaagt agtatttaat tgttactagt
     3421 atagtaaagc tcattttaat tgtcaagttc ttttgaacat agcggataca tttttcttct
     3481 aagaaccaag cctaagtttt tttaaaaagt cacaaaatga ggtatccctg caaaaaacct
     3541 tttctttctt ggaatttctt tgttataatg ctttacagag gtgaaaaaat gaaaactatt
     3601 gcttttgatt ctaacaagta tttaaacttg caaagagatc atattttaga acgtatcagc
     3661 caatttgatg gcaaacttta catggaattt ggtggtaaaa tgttagaaga ttaccatgct
     3721 gcccgtgtac ttcctggcta tgaacctgac aataagatta aattactcaa agagctcaaa
     3781 gaacaagtcg aaattgtaat tgccattaac gctaataaca ttgaacattc taaagcgcgt
     3841 ggtgatttag gcatttctta tgaccaagaa gtctttcgct taattgacaa atttaacacg
     3901 ttagatattt acgtaggttc tgtcgttatt acccaataca ataaccaacc cgctgcagat
     3961 gcctttcgca agcaactcaa aaaaaatggc attgcttctt acctgcatta tcctatcaag
     4021 agctacccga cagatattaa tcatattatc tcatctgagg ggatggggaa aaatgattac
     4081 atcaaaacga gtcgtaacct tatcgttgtt acagcaccag gccctgggtc tggaaaatta
     4141 gccacttgta tatcccaaat gtatcacgac caaatcaatg gggttaagtc tggctatgct
     4201 aaatttgaaa ccttcccagt ttggaatctt cctcttcatc accctgtcaa tctggcttat
     4261 gaggcagcca cggcagacct agatgatgtg aatatgattg accccttcca tttggaaact
     4321 tatggcaaaa cggccgttaa ttacaaccgt gatatcgagg tctttcctgt cttaaatcgg
     4381 accttcgagc gtattttgag caagtctcct tatgcctcac caaccgatat gggtgttaac
     4441 atggttggtt tttctatcgt caatgaagaa gctgctatcg aagcttctaa acaagaaatc
     4501 atccgtcgtt attaccaaac cctcgtggat tttaaggcag agcgggtcac agaatctgct
     4561 gtcaaaaaaa ttgagctctt gatgaatgat atcggtgtca cccctgacga tcgtcacgtt
     4621 actgtagctg ctcatcaaaa agcagagcaa acaggccaac ctgcccttgc tctccaacta
     4681 ccaaatgggc aaattgtgac aggtaaaaca tctgaactct ttggaccaac tgcagctgtt
     4741 atcatcaatg cgattaaaac cttggcaaag attgataaaa ccactcattt gattgaaccc
     4801 gaatacgtga aacctattca agggctcaaa gtgaaccact taggcagtca caatccgcgg
     4861 cttcattcca atgaaattct gattgcgtta gccattactg ctatgactag tgaagaagct
     4921 aatctagcca tgaaagaact cggcaatttg aaaggtagcg aagcccattc taccgttatc
     4981 ttaaccgaag aagataaaaa tgtactccga aaacttgggg taaatattac ttttgaccct
     5041 gtttaccaac atcacaaact ttaccgtaaa taaactataa ccacccgtct aacgggtgat
     5101 ttatggtatc tctcaataaa ggaaaaaagt cccaattctc agagagttgg gacttttttc
     5161 ctttattgat tcattaatca cccgttatga cgtcggccag tcacaaacat ggtcaagctt
     5221 gtttttgtta gaagagctcc tgtttggtta tgaatcgtca catcaaccac ctgcgtggtt
     5281 cgacctccat gaaccaatcg accttcaacc atcagcttgt ctcccttgtg ccctgctttt
     5341 aaatagttag cattggcctg taaggtgaca gattctacgc cagtagttct agcaaccaaa
     5401 ccacctactt gatcacatag agtgaaaaga taaccaccat gagcattccc atagtaattc
     5461 aaagccgtct cagtcacttc agtacttaaa ataaggtgtc ctttttcagc aagttcaatt
     5521 tggtaattat caaaaatact aataacattt aaggtcatct cttgtgttaa atctgacatt
     5581 gtttttctct tttctaagct tagtctaaaa gcaatacaag cctcattata ctacaaatag
     5641 gaacagaagg cagagctcat gtttcaaaaa tcagtagcct ttccatcctt ttctagcgta
     5701 ttgaacactt ccaagcataa atgaagcgca aaactaaagg aatcagcgcc ccggttgcgt
     5761 tgatcgtaaa cctctacatc tgctagagta tctgctgtaa ataagagttg tccccaaagg
     5821 atgcctctta attgagctac tgctgctaag gcagaacatc ccatctcaac aaccgaacac
     5881 ccttcttcct ggcgataagc cactttttct ttggtttctc ggtaaaaacc atctgttgac
     5941 caggtgatga cctcttgata agctaacccc tgtgctgaaa gcgttttctc tatcggtcta
     6001 agcatcttag gatcaatatc aatataacaa gatggtgcca cgtaatgata acttgttcct
     6061 tcatctcgca aagccttaac tggcactaaa aaacggtttt ctgcgatagg aaccaaaacg
     6121 ccacaggttc ctgtactgat catttcttta accccataac caatcagcca gtccatgaat
     6181 tgcgccgcag gagctgagcc aacgggagct tgagctaggc aaatgtcttg gcctttataa
     6241 ggtatctgat aaacagggta aacctttgtg gctgaaatga attctgctac ttttttacct
     6301 tgatggtcta aagcaaaagc ttcgatctcg tcttctaaga aagcatagac acacttagct
     6361 gtagaccttc atgtgtaggt ataatgacgg ctgatggatt actatcacat tctaaaaggg
     6421 gaatctcgtg tttggtaata ggcattcgtt tctccttatt tttgtcatgt ggaggcagct
     6481 gccttactcc ttgattagca acactacgca ctcgacgtga tgggtctgcg ggagtcaaaa
     6541 ggtccagtgg accttttgag cgtctcacct tgaaataaca gagtgaggcg gactctttaa
     6601 ctccgtttct caagtaaact tacacactca acttgttcat tattatccaa acttatattc
     6661 aagtcatcgt ctataattgg gagcttgaaa gtaattgatt ttagccattg tccgttaggt
     6721 tgtttttctt cataaacttg aatttcagaa atcaaagctg taattaactg tctacgctct
     6781 acatcattca tgactttata gagtttatca aaatagatca gaaccttata tatgttatct
     6841 cctgtgagtt tttcagcttc aatagtctgt ttctttgctt tcgcatcaat tagtgatgat
     6901 tctaattcat ctattttgtc atacatacga tatagtctat catctaaatc ctgtttcctt
     6961 ctcttatagt gcttatcttc aacatctaaa ttatctattt cctcaattat cttaaacttt
     7021 gtagaatgac tcttcctcaa ttccttttgg taattatcta tttctttttc tatttcagag
     7081 gtgtccacct tcatgttgat tttttcttgc atcatagaag caaatttcgg attacttaca
     7141 atcttgacaa tcacctctgc aacagcatca tctaacaatt cttctctaat ttgcttacgg
     7201 aatgtacact tatgacctct tatcatctgc ctatgtttac aaccatagta ataaaaatct
     7261 ttatactttg tgccatcttt ctttttcttg gtacacttgt ttccaaacat tcccgctcca
     7321 catatagggc attttacaat tccagaaagc aagtgcgtgc gtgtatcttt tcctttattc
     7381 acatgctcat atttctttgc ttgagatttt agcttaacct gagcagcttg ccaaacttcg
     7441 tcggaaacta tagcttcatg tataccttca gatattagat attcatcttg ttcaacctgc
     7501 ttatattcat ttcttgtacc atgaactttt tctaaagttc ttcttccaaa tgctattttc
     7561 ccattatata caggattctt taatatcttt cttataagtc ctgcatcaaa caaaggattc
     7621 tttccattct gtcttgggat ttttctaatt ccatgattct ctaagtattt agatagccca
     7681 ttggctccta tcgtagtatt tacatactgg tcgaaaatcg ttcttatggc aactgcctct
     7741 tcctcattta taaacagctt gccatcttca agtttatatc catacggagc aaagccacca
     7801 ttccattttc cttcccttgc tttttgaatg cgaccttcca ttgtttgaat acggatgttt
     7861 tctctttcta tttcagccac agctgataaa acagaaatca ttagtttccc agcatcttta
     7921 gatgaatcaa tgccatcttc aacgcagata agattaactc cataatcctg cattatctga
     7981 agtgtagaaa gaacatcagc ggcatttctt gcaaatcttg ataacttaaa cacaagaaca
     8041 aaagacactc catcttttcc agattttata tcttccatca ttcgattgaa ctgttttcta
     8101 ccttcaatag acttgccaga cttcccggca tcttcatact ctccgacaat ttcataatcg
     8161 ttgtaaatag caaaagcttt cattcgtgat ttttgtgcct ctaacgaata cccctctatc
     8221 tgtattgacg tagatactcg tgtatagagg tatactttta ttttttcttt tgacatagca
     8281 t
//
LOCUS       DATXIG010000055         8260 bp    DNA     linear   BCT 28-DEC-2023
DEFINITION  TPA_asm: Streptococcus pyogenes strain MGAS11113
            SAMN08555799-rid19682583.denovo.055, whole genome shotgun sequence.
ACCESSION   DATXIG010000055 DATXIG010000000
VERSION     DATXIG010000055.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN08555799
            Sequence Read Archive: SRR6740495
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pyogenes
  ORGANISM  Streptococcus pyogenes
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 8260)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8260)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-OCT-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-OCT-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001918058.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001918058.2
            Genome Coverage       :: 368x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/12/2023 11:23:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 1,806
            CDSs (total)                      :: 1,769
            Genes (coding)                    :: 1,722
            CDSs (with protein)               :: 1,722
            Genes (RNA)                       :: 37
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1, 1 (5S, 23S)
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 47
            CDSs (without protein)            :: 47
            Pseudo Genes (ambiguous residues) :: 0 of 47
            Pseudo Genes (frameshifted)       :: 30 of 47
            Pseudo Genes (incomplete)         :: 20 of 47
            Pseudo Genes (internal stop)      :: 9 of 47
            Pseudo Genes (multiple problems)  :: 11 of 47
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8260
                     /organism="Streptococcus pyogenes"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN08555799-rid19682583.denovo.055"
                     /strain="MGAS11113"
                     /serotype="Emm28"
                     /isolation_source="normally sterile site"
                     /host="Homo sapiens"
                     /db_xref="taxon:1314"
                     /country="Finland"
                     /collection_date="2002"
                     /collected_by="National Public Health Laboratory"
     gene            162..860
                     /locus_tag="VC122_001408"
     CDS             162..860
                     /locus_tag="VC122_001408"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011184534.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HEP5641327.1"
                     /translation="MFSGHQLKTARLSKGITQSELGRLLHVNKMTISNWEKGKNIPNE
                     KHLNALLHLFNVTSDYFDPNYRLLTPYNQLTISNKEKVIGYSERLLNHQIDKKSKDLI
                     DKPSQLYAYRVYESLSAGTGYSYFGDGNFDVVFYDEQLEYDFASWVFGDSMEPTYLNG
                     EVVLIKQNSFDYDGAIYAVEWDGQTYIKKVFREDEGLRLVSLNKKYSDKFAPYSEEPR
                     IIGKIIANFRPLEI"
     gene            complement(995..2557)
                     /gene="ffh"
                     /locus_tag="VC122_001409"
     CDS             complement(995..2557)
                     /gene="ffh"
                     /locus_tag="VC122_001409"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012560729.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="signal recognition particle protein"
                     /protein_id="HEP5641328.1"
                     /translation="MAFESLTQRLQDVFKHIRGKKKLSESDVQEVTKEIRLALLEADV
                     ALPVVKTFIKRVRERAIGHEIIDTLDPTQQILKIVNEELTSILGSETAEIDKSPKIPT
                     IIMMVGLQGAGKTTFAGKLANKLIKEENARPLMIAADIYRPAAIDQLKTLGQQINVPV
                     FDMGTDHSAVDIVRKGLEQARENRNDYVLIDTAGRLQIDEKLMEELRDVKALAQPNEI
                     LLVVDSMIGQEAANVAYEFNHQLSITGVVLTKIDGDTRGGAALSVREITGKPIKFTGI
                     GEKITDIETFHPDRMSSRILGMGDLLTLIEKASQEYDEKKSLELAEKMRENTFDFNDF
                     IEQLDQVQNMGPMEDLLKMIPGMAGNPALANIKVDENQIARKRAIVSSMTPAERENPD
                     LLNPSRRRRIAAGSGNSFVEVNKFIKDFNQAKSMMQGVMSGDMSKMMKDMGINPNNLP
                     KNMPAGMPDMSSLEGMMGQGGMPDLSGLGGDMDMSQVFGKGFKGKIGQFAMKQAMKRQ
                     ANKLKKAKKKRK"
     gene            complement(2572..2913)
                     /locus_tag="VC122_001410"
     CDS             complement(2572..2913)
                     /locus_tag="VC122_001410"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002984591.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative DNA-binding protein"
                     /protein_id="HEP5641329.1"
                     /translation="MNIMEIEKTNRMNALFEFYAALLTDKQMNYIELYYADDYSLAEI
                     ADEFGVSRQAVYDNIKRTEKILETYEMKLHMYSDYVVRSEIFDDMIAHYPHDEYLQEK
                     ISILTSIDNRE"
     gene            complement(3002..3700)
                     /locus_tag="VC122_001411"
     CDS             complement(3002..3700)
                     /locus_tag="VC122_001411"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000936162.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HEP5641330.1"
                     /translation="MLPAYIKIHDAIKKDIDLGIWPIGSRLPSERHLAEHFTVSRMTL
                     RQAITLLVEEGILERRIGSGTYVASHRVQEKMRGTTSFTEIIRSQGRQPSSKLLSYQK
                     QLASDTEVKELNLDKTDLVIRMERIRYADSVPLVYEIASIPEKFIKTVKRADITEHFF
                     HSLIANGYEIGKSKQTIYAKLASERVASYLEVAKGHAILALTQVSYFTDGKPFEYVRS
                     QYIGDRFEFYLENN"
     gene            complement(3783..5216)
                     /locus_tag="VC122_001412"
     CDS             complement(3783..5216)
                     /locus_tag="VC122_001412"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011888856.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEP5641331.1"
                     /translation="MLRKKRILGLFRPVELIFLGLLLSLVVSYLAWTNSFATLHNILA
                     TVGIVERSKDQQPRYHIGQAIQVQKSGPYHQWIGTINKQVEDIAENYRVSYHYEVVFP
                     IGKVTVSLPEHNLKEPDKPRFKKGDIVKLSSLTKKPHIKVYQGQLATIKQVKKRYDYS
                     LGGYQYDINLKDNLRLDGISEQDFVKPYYIRFNKGNSPEQNNRLLRKAFAYAKQHPNS
                     VISFPKGQFHIGSLPSQKDYFELPSDTAIIGHQTEFIIHGKMLWFGFPTGPKAEQGVR
                     NLVLTGVHFKANDLKKGDHFMIMADHGTDWHIYDNKFTMVHKRNSHIFDLGSLQNSLF
                     EKNQFIGYAPELVQDQQLLSKAQGHDFFSEVIQFDAAVHHFAWDGGLLSNIAPNYEAF
                     NQTRHLCHNITVSQNQFLPYIDPTGCLRAYSGSIGQHSSKVGVIRVLNNVFTSSIVTK
                     AKLTSWFMEPIHFPPNSPVIVAGNIIN"
     gene            5383..6945
                     /gene="guaA"
                     /locus_tag="VC122_001413"
     CDS             5383..6945
                     /gene="guaA"
                     /locus_tag="VC122_001413"
                     /EC_number="6.3.5.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009880616.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine-hydrolyzing GMP synthase"
                     /protein_id="HEP5641332.1"
                     /translation="MTEISILNDVQKIIVLDYGSQYNQLIARRIREFGVFSELKSHKI
                     TAQELREINPIGIVLSGGPNSVYADNAFGIDPEIFELGIPILGICYGMQLITHKLGGK
                     VVPAGQAGNREYGQSTLHLRETSKLFSGTPQEQLVLMSHGDAVTEIPEGFHLVGDSND
                     CPYAAIENTEKNLYGIQFHPEVRHSVYGNDILKNFAISICGARGDWSMDNFIDMEIAK
                     IRETVGDRKVLLGLSGGVDSSVVGVLLQKAIGDQLTCIFVDHGLLRKDEGDQVMGMLG
                     GKFGLNIIRVDASKRFLDLLADVEDPEKKRKIIGNEFVYVFDDEASKLKGVDFLAQGT
                     LYTDIIESGTETAQTIKSHHNVGGLPEDMQFELIEPLNTLFKDEVRALGIALGMPEEI
                     VWRQPFPGPGLAIRVMGAITEEKLETVRESDAILREEIAKAGLDRDVWQYFTVNTGVR
                     SVGVMGDGRTYDYTIAIRAITSIDGMTADFAQLPWDVLKKISTRIVNEVDHVNRIVYD
                     ITSKPPATVEWE"
     gene            complement(6987..8210)
                     /locus_tag="VC122_001414"
     CDS             complement(6987..8210)
                     /locus_tag="VC122_001414"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002984581.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminoacyltransferase"
                     /protein_id="HEP5641333.1"
                     /translation="MTNLTFYAKIGISEEEHDSFVKEHQQISVLQGSDWAKIKNQWQN
                     ERIGIYKEEKQVASLSLLIKLLPLGRSIIYIPRGPVMDYLDRDLVAFTMKTLKDYGKT
                     KKALFIKYDPAILLKQYALGQEEEEKPLALAAIKNLQEAGVHWTGLTMEIADSIQPRF
                     QANIYTQENLEMQFPKHTRRLIKDAKQRGVETYRASQSELHKFSKIVSLTEKRKNISL
                     RNEAYFQKLMTTYGDKAYLHLAKVNIPQKLDQYRQQLNLINQDITRTQAHQKKRLKKL
                     EDQKASFERYITEFEGFTDQYPEEVVVAGILSISYGNVMEMLYAGMNDDFKKFYPQYL
                     LYPKVFQDAYQDGIIWANMGGVEGSLDDGLTKFKANFAPTIEEFIGEFNLPVSPLYHI
                     ANTMYKIRKQLKNKH"
BASE COUNT         2457 a         1577 c         1479 g         2747 t
ORIGIN      
        1 agacctgcta gtacacgaat ctagtcgcta tgttttaggt gtttcccctt tttgacagta
       61 ttgctatcac atgaattaat tgtaggaaat gttacacttg ctttcgtttt atttttttga
      121 tactattagt aaagttataa ctttactaaa ggagctacca aatgttttct ggacatcaac
      181 taaaaacagc acgattatca aagggaataa ctcaatcaga attgggaaga ttgttgcatg
      241 tcaataaaat gacaatatct aattgggaaa aaggtaagaa tataccaaat gaaaaacatt
      301 taaatgcctt attgcatcta ttcaatgtga catctgatta tttcgaccca aactatagat
      361 tgctaacgcc ttataaccag ctgacaatat ctaataaaga aaaagtaatt ggctattcag
      421 agcgattgtt aaatcatcaa atagacaaaa aatctaaaga tctcatagat aaaccatcac
      481 aattatatgc ttatcgggtc tatgaaagtt tatctgctgg tactggttac tcctattttg
      541 gtgatggtaa ctttgatgtt gtcttttacg atgaacaatt agaatacgat tttgcgtctt
      601 gggtttttgg agattctatg gagccaactt atttaaatgg cgaagttgtt cttataaaac
      661 aaaatagttt tgattacgat ggagcaattt atgcagtcga atgggatggg caaacatata
      721 tcaaaaaggt atttcgtgaa gatgagggat tacgtctagt gtccttaaat aaaaaatatt
      781 ctgataagtt tgctccctat agcgaagaac ctcgcattat tggcaaaatt atcgctaatt
      841 ttaggccctt agaaatttaa gttagattga ttaggttatt tcaaatttta aatgaactca
      901 tcatgcatgt aacgcggtct tccataattt taataatctt gtatacaaga taaatgaatt
      961 tcacatcaaa aagagacgaa aatttccgtc tcttttattt tcgttttttc tttgcttttt
     1021 ttagtttgtt agcttgacgt ttcatggcct gtttcatggc aaattgtcca attttcccct
     1081 tgaacccttt accaaatact tgactcatat ccatatcacc accaaggcca gataaatcgg
     1141 gcataccacc ttgtcccatc atcccttcta aggaagacat atctggcata ccagcaggca
     1201 tgtttttagg aagattattg ggattaatgc ccatatcttt catcatctta gacatatcgc
     1261 ccgacataac cccttgcatc atgcttttgg cttggttaaa gtctttgata aatttattaa
     1321 cttccacaaa gctgttcccg gaaccagctg caatacgacg gcgacgactc gggtttagta
     1381 agtcagggtt ttcacgttca gcgggggtca tcgaagagac aatagcacgt ttgcgagcaa
     1441 tttggttttc gtcaactttg atattggcca aagctgggtt accagccatt cctggaatca
     1501 ttttcaagag gtcttccata ggccccatat tttgcacttg atctaactgt tcaatgaaat
     1561 cattgaaatc aaaggtgttc tcacgcattt tttcagctaa ctctaaggat tttttctcat
     1621 catattcctg gctagctttt tcaataagag tgagtaagtc acccattccc aaaatccgac
     1681 tggacatgcg gtctgggtga aatgtttcaa tatctgtgat tttttcacca atacctgtaa
     1741 atttaattgg ttttccagtg atttcacgga cagatagtgc tgcacctcca cgggtatcac
     1801 catcaatctt tgttaacaca actcctgtga tgctcaactg atgattaaac tcgtaggcta
     1861 cattggcagc ttcttgacca atcatgctat ctacaaccaa aagaatttca tttggttgtg
     1921 ctaaagcctt gacatcgcgc agctcttcca tcagtttttc atcaatttgc aagcgacccg
     1981 cagtatcaat caagacatag tcattgcggt tttcacgagc ttgttccaaa ccttttctga
     2041 caatatcgac tgctgagtgg tctgtcccca tatcaaaaac aggtacattg atttgttgcc
     2101 ccagtgtttt taactgatca atggccgctg gacggtaaat atcggcagca atcatcagtg
     2161 gtcgcgcgtt ttcttcttta attaatttat tagcgagttt tcctgcaaaa gtggttttac
     2221 cagccccttg caaaccgacc atcatgataa tagttggaat ttttggagat ttatcaatct
     2281 ccgcggtttc tgagcctaaa atactcgtta attcttcatt aacaatctta agaatttgtt
     2341 gtgttggatc aagagtatca ataatctcat ggccaatagc gcgctcacgt acgcgcttaa
     2401 taaaggtttt aacaactggt aaagcgacat cggcttctaa aagagctaga cgaatctctt
     2461 ttgtcacttc ttgaacatct gattctgata atttcttttt accgcgaatg tgtttaaaca
     2521 catcttgcaa acgttgggtt aagctttcaa aagccatagt caaaactcct cttactctct
     2581 attgtcaata ctggttagaa tggaaatttt ttcttgcaaa tactcatcat gagggtagtg
     2641 agcaatcatg tcgtcaaaga tttcacttcg aacaacataa tccgaataca tatgaagttt
     2701 catctcataa gtctctaaaa ttttttccgt acgcttaata ttatcataga cagcctgacg
     2761 actgacacca aattcatcag caatctcagc taagctataa tcatcggcat aatacagctc
     2821 aatataattc atttgtttat ctgttagcaa agctgcataa aattcaaaaa gggcattcat
     2881 cctgtttgtt ttttcaattt ccataatgtt cattttatca tactatgatt gtttttaaca
     2941 gtagcaaatt aattgcctta tgctgttcgt gtaaaaaagg aagtcataaa tgacttcttt
     3001 actaattatt ttctaagtaa aattcaaaac ggtcaccgat atactgactg cggacatact
     3061 caaaaggttt gccgtctgta aagtaagaca cctgagttag agccaaaatg gcatgtcctt
     3121 tagcaacttc caagtaggag gctacacgct cacttgctag cttagcgtag attgtctgtt
     3181 tacttttacc aatctcgtaa ccatttgcta tcaatgagtg aaaaaagtgt tctgtaatat
     3241 cagctcgttt aaccgttttt ataaattttt ctgggattga agcaatttcg tacaccaaag
     3301 ggacactgtc tgcatagcgg atacgttcca ttcgaatgac caagtctgtt ttgtctaagt
     3361 tcaattcttt aacttcggta tcactagcta attgcttttg ataggataat aatttagagg
     3421 agggttgtcg tccttgggag cgtataattt ctgtgaaact tgttgttcct cgcatttttt
     3481 cttgaacacg atgactggca acataagtac cacttcctat ccgcctttca agaatccctt
     3541 cttctactag taatgtaata gcctgccgta gagtcattcg actaacggtg aaatgttccg
     3601 ctaaatgtcg ttcacttggt aagcgactac caattggcca gatgcccaga tcaatgtctt
     3661 ttttaattgc atcatgaatt tttatataag ctggtaacat gccacactcc tcaagtcggt
     3721 tattcatcta ttttatcata tttttgatga aaattgaatt gtaaatattg aggtatttgg
     3781 catcaattaa tgatattacc tgcgacaata actggtgaat ttggtggaaa atgaataggt
     3841 tccataaacc aactagtgag cttcgcttta gtaacaatgg atgaggtaaa aacattattt
     3901 aaaaccctaa taactcctac ttttgaggaa tgctgaccaa tagaaccact ataggctctc
     3961 aggcaaccag tcggatctat ataaggtaaa aattgatttt ggcttacagt aatattgtga
     4021 catagatgtc gagtttggtt aaatgcttca tagtttggag caatattact aagtagacct
     4081 ccatcccatg caaaatgatg aacagcagca tcaaactgaa tgacttctga aaaaaaatca
     4141 tgcccttgag cctttgatag cagctgttgg tcttgtacta attctggcgc atagccaata
     4201 aattggtttt tctcaaacaa tgaattttgt agagatccta aatcaaaaat atgactatta
     4261 cgcttatgaa ccatagtaaa tttgttatcg taaatatgcc aatcagtacc atgatcagcc
     4321 ataatcataa agtggtctcc ttttttcaaa tcatttgctt tgaaatgcac tccagtcaac
     4381 acgagattac gaacaccttg ttcagccttt ggtcctgtag ggaatccaaa ccacaacatt
     4441 ttaccgtgaa taatgaactc tgtctgatga ccaataatag ctgtatcaga tggaagctca
     4501 aaataatctt tttgtgaagg caaagagcca atgtgaaatt gcccctttgg aaaagatata
     4561 acgctatttg gatgctgctt tgcataagcg aaagcttttc gcaaaagacg attgttttgc
     4621 tcaggggaat ttcctttatt gaacctaata taataaggtt taacaaaatc ttgctctgaa
     4681 attccatcta atcttagatt atctttcagg ttaatatcgt actgatatcc tcctaacgaa
     4741 tagtcatagc gttttttaac ttgtttaata gtcgctaatt gaccttgata cacttttata
     4801 tgtggctttt tagttaatga agataattta acgatatctc cttttttaaa cctcggttta
     4861 tcaggctctt tcaggttatg ttctggcaaa gaaacagtga cttttcctat tggaaatacc
     4921 acttcataat gataactcac tcggtaattt tcggctatat cttctacctg tttattaata
     4981 gttccaatcc attgatgata tggaccactt ttttgtactt gaatagcttg tccaatgtga
     5041 tagcgaggct gttgatcttt gcttcgttcc actattccga cagttgctaa tatattgtga
     5101 agtgttgcaa aactattggt ccacgcaagg tacgaaacaa ctaaactcaa caataatcct
     5161 aggaaaatta attcaacagg tcgaaataaa cccaaaattc tcttttttct tagcataggc
     5221 cctccctgtt gtcttaattc cctttcatag cctctattgt aacataacga ttacgttttt
     5281 ttccaacgag ctagagaaat tgtaatgtct tttcgatgaa aatatgctac aataagttga
     5341 caactatcag cctgacggtt ggtatagaat gaaaggatta taatgactga aatttcaatt
     5401 ttgaatgatg ttcaaaaaat tatcgttctt gattatggta gccagtacaa tcagcttatt
     5461 gctagacgta ttcgagagtt tggtgttttc tccgaactaa aaagccataa aatcaccgct
     5521 caagaacttc gtgagatcaa tcccataggt atcgttttat caggagggcc taactctgtt
     5581 tacgctgata acgcctttgg cattgaccct gaaatctttg aactagggat tccgattctt
     5641 ggtatctgtt acggtatgca attaatcacc cataaattag gtggtaaagt tgttcctgct
     5701 ggacaagctg gtaatcgtga atacggtcag tcaacccttc atcttcgtga aacgtcaaaa
     5761 ttattttcag gcacacctca agaacaactc gttttgatga gccatggtga tgctgttact
     5821 gaaattccag aaggtttcca ccttgttgga gactcaaatg actgtcccta tgcagctatt
     5881 gaaaatactg agaaaaacct ttacggtatt cagttccacc cagaagtgag acactctgtt
     5941 tatggaaatg acattcttaa aaactttgct atatcaattt gtggcgcgcg tggtgattgg
     6001 tcaatggata attttattga catggaaatt gctaaaattc gtgaaactgt aggcgatcgt
     6061 aaagttcttc taggtctttc tggtggagtt gattcttcag ttgttggtgt tctacttcaa
     6121 aaagctatcg gtgaccaatt aacttgtatt ttcgttgatc acggtcttct tcgtaaagac
     6181 gagggcgatc aagttatggg aatgcttggg ggcaaatttg gcctaaatat tatccgtgtg
     6241 gatgcttcaa aacgtttctt agaccttctt gcagacgttg aagatcctga gaaaaaacgt
     6301 aaaattattg gtaatgaatt tgtctatgtt tttgatgatg aagccagcaa attaaaaggt
     6361 gttgacttcc ttgcccaagg aacactttat actgatatca ttgagtcagg aacagaaact
     6421 gctcaaacca tcaaatcaca tcacaatgtg ggtggtctcc ccgaagacat gcagtttgaa
     6481 ttgattgagc ccttaaacac tcttttcaaa gatgaagttc gagcgcttgg aatcgctctt
     6541 ggaatgcctg aagaaattgt ttggcgccaa ccatttccag gtcctggact tgctatccgt
     6601 gtcatgggag caattactga agaaaaactt gaaaccgttc gcgaatcaga cgctatcctt
     6661 cgtgaagaaa ttgctaaggc tggacttgat cgtgacgtgt ggcaatactt tacagttaac
     6721 acaggtgtcc gttctgtagg cgtcatggga gatggtcgta cttatgatta taccatcgcc
     6781 attcgtgcta ttacgtctat tgatggtatg acagctgact ttgctcaact tccttgggat
     6841 gtcttgaaaa aaatctcaac acgtatcgta aatgaagttg accacgttaa ccgtatcgtc
     6901 tacgacatca caagtaaacc acccgcaaca gttgaatggg aataacagaa taaacattaa
     6961 aagactcgct cattttggcg agtcttttaa tgtttattct ttaactgttt tcgtattttg
     7021 tacatggtat tagcaatatg ataaagtggg ctgacaggga gattaaattc tcctataaat
     7081 tcttctattg tcggagcaaa gttggcctta aatttggtaa gtccatcatc aagcgagcct
     7141 tctactcctc ccatgttagc ccaaataata ccatcttgat aagcatcctg aaaaacttta
     7201 ggatacagca gatactgagg ataaaacttc ttaaaatcat cattcatccc agcataaagc
     7261 atttccataa catttccata agaaatagat aatatacctg ctacaacaac ttcctcagga
     7321 tattggtctg taaagccttc aaattcagtt atataacgtt caaaagaagc tttttgatct
     7381 tctaattttt ttaaacgctt cttttgatga gcttgggtgc gagtaatatc ttggttaata
     7441 agatttaatt gctggcggta ttgatctagt ttttgaggaa tattcacttt tgctagatgt
     7501 aagtaggcct tatccccata agtagtcatc aacttttgaa agtaagcttc gttacgcaaa
     7561 gaaatatttt tacgtttttc tgttaaggag acaatcttgg aaaatttgtg aagttctgat
     7621 tgactggcac gatatgtttc tacaccacgc tgcttagcat cttttattaa acgtctggta
     7681 tgcttaggaa attgcatctc aaggttttct tgagtgtaaa tattagcttg gaaacgaggt
     7741 tggatactat ctgcaatctc cattgttaaa ccagtccaat gaacaccagc ttcttggaga
     7801 ttcttaatag ctgctaaagc taaaggtttt tcttcttctt cctgtcccag tgcgtattgt
     7861 tttaacagga tagctggatc atatttgata aagagggcct ttttagtttt accataatcc
     7921 tttagtgttt tcatggtaaa tgccaccaaa tcacggtcaa gataatccat gactggccct
     7981 cttggaatat aaataatgct tcttccaagt ggcaatagct taatcaaaag tgataaagag
     8041 gcaacctgct tttcctcttt atagatacca attcgctcat tctgccattg atttttgatt
     8101 tttgcccaat cactaccttg taaaacgcta atttgctgat gttctttaac aaaagagtca
     8161 tgctcttctt ctgaaatacc gatttttgca taaaacgtta aattcgtcat ttattgtccc
     8221 catctattcg taatcttagc agatgtactt actaagtatc
//
LOCUS       DATXIG010000056         7969 bp    DNA     linear   BCT 28-DEC-2023
DEFINITION  TPA_asm: Streptococcus pyogenes strain MGAS11113
            SAMN08555799-rid19682583.denovo.056, whole genome shotgun sequence.
ACCESSION   DATXIG010000056 DATXIG010000000
VERSION     DATXIG010000056.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN08555799
            Sequence Read Archive: SRR6740495
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pyogenes
  ORGANISM  Streptococcus pyogenes
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 7969)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7969)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-OCT-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-OCT-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001918058.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001918058.2
            Genome Coverage       :: 368x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/12/2023 11:23:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 1,806
            CDSs (total)                      :: 1,769
            Genes (coding)                    :: 1,722
            CDSs (with protein)               :: 1,722
            Genes (RNA)                       :: 37
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1, 1 (5S, 23S)
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 47
            CDSs (without protein)            :: 47
            Pseudo Genes (ambiguous residues) :: 0 of 47
            Pseudo Genes (frameshifted)       :: 30 of 47
            Pseudo Genes (incomplete)         :: 20 of 47
            Pseudo Genes (internal stop)      :: 9 of 47
            Pseudo Genes (multiple problems)  :: 11 of 47
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7969
                     /organism="Streptococcus pyogenes"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN08555799-rid19682583.denovo.056"
                     /strain="MGAS11113"
                     /serotype="Emm28"
                     /isolation_source="normally sterile site"
                     /host="Homo sapiens"
                     /db_xref="taxon:1314"
                     /country="Finland"
                     /collection_date="2002"
                     /collected_by="National Public Health Laboratory"
     gene            17..883
                     /locus_tag="VC122_001415"
     CDS             17..883
                     /locus_tag="VC122_001415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011284822.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter substrate-binding
                     protein PstS family protein"
                     /protein_id="HEP5641334.1"
                     /translation="MKMKKKFFLLSLLVLSTFFLSACSSWIDKGESITAVGSTALQPL
                     VEAVADEFGSSNLGKTVNVQGGGSGTGLSQVQSGAVQIGNSDVFAEEKDGIDASKLVD
                     HQVAVAGLAVIANPKVKVSNLSSQQLQKIFSGEYTNWKQVGGEDLAISVINRAASSGS
                     RATFDSVIMKGVNAKQSQEQDSNGMVKSIVSQTPGAISYLSFAYVDSSVKSLQLNGFK
                     ANAKNVATNDWPIWSYEHMYTKDKPTGLTKEFLDYMFSDEVQQNIVTHMGYISINDME
                     VVKSHDGKVTKR"
     gene            894..1829
                     /gene="pstC"
                     /locus_tag="VC122_001416"
     CDS             894..1829
                     /gene="pstC"
                     /locus_tag="VC122_001416"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019323233.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter permease subunit PstC"
                     /protein_id="HEP5641335.1"
                     /translation="MENQELAKKLASPSKNSRLETFGRTITFLCLALIVFIVAMILIF
                     VAQKGLSTFFVDKVNLFDFLFGKEWQPSVKNAAGIPYLGALPMITGSFLVTILSAIIA
                     TPFAIGAAVFMTEISPKYGAKLLQPAVELLVGIPSVVYGFIGLQVIVPFMRSIFGGTG
                     FGILSGVCVLFVMILPTVTFMTTDSLRAVPRHYREASMAMGATCWQTIWRVVLNAARP
                     GIFTAIIFGMARAFGEALAIQMVVGNSAVMPSSLTTPAATLTSVLTMGIGNTVMGTVQ
                     NNVLWSLALVLLLMSLAFNSLVKLITKERKRNYER"
     gene            1819..2706
                     /gene="pstA"
                     /locus_tag="VC122_001417"
     CDS             1819..2706
                     /gene="pstA"
                     /locus_tag="VC122_001417"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008088954.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter permease PstA"
                     /protein_id="HEP5641336.1"
                     /translation="MNAKKVDKVATGTLYTIAGIIVAILASLILYILVRGLPHISWSF
                     LTGKSSSYEAGGGIGIQLYNSFFLLIVTLIISIPLSTGAGIYLAEYAKKGPVTNFIRT
                     CIEILSSLPSVVVGLFGYLIFVVQFEYGFSIISGSLALTVFNLPQMTRNVEDSLLHVH
                     HTQREAGLALGLSRWETVFYVVIPEALPGMVTGIVLASGRIFGEAAALIYTAGQSAPA
                     LDWSNWNPLSVTSPISIFRQSETLAVHIWKVNSEGTIPDATLVSAGSAAVLLIFILIF
                     NFSARFIGKKLHSKMTAAK"
     gene            2722..3525
                     /gene="pstB"
                     /locus_tag="VC122_001418"
     CDS             2722..3525
                     /gene="pstB"
                     /locus_tag="VC122_001418"
                     /EC_number="7.3.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000852852.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter ATP-binding protein"
                     /protein_id="HEP5641337.1"
                     /translation="MTEYNWNERHIITFPEETLALATKDLHVYYGAKEAIKGIDMQFE
                     KHKITALIGPSGCGKSTYLRSLNRMNDTIDIARVTGEILYQGIDVNRKDMNVYEIRKH
                     LGMVFQRPNPFAKSIYKNITFAHERAGVKDKKVLDEIVETSLKQAALWDQVKDDLHKS
                     AFTLSGGQQQRLCIARAISVKPDILLMDEPASALDPIATMQLEETMFELKKNYTIIIV
                     THNMQQAARASDYTAFFYLGNLIEYDKTRNIFQNAQCQSTNDYVSGHFG"
     gene            3538..4296
                     /gene="pstB"
                     /locus_tag="VC122_001419"
     CDS             3538..4296
                     /gene="pstB"
                     /locus_tag="VC122_001419"
                     /EC_number="7.3.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014294590.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter ATP-binding protein"
                     /protein_id="HEP5641338.1"
                     /translation="MTEPILQIRDLSVYYNQKKTLKDVSLDLYPNEITALIGPSGSGK
                     STLLRSINRMNDLNPEVTITGSIVYNGHNIYSPRTDTVDLRKEIGMVFQQPNPFPMSI
                     YENVVYGLRLKGIRDKSILDHAVESSLKGASIWNEVKDRLHDSAVGLSGGQQQRVCIA
                     RVLATSPRIILLDEPTSALDPISAGKIEETLLLLKKDYTLAIVTRSMQQASRLSDRTG
                     FFLEGDLLECGPTKAMFMNPKRKETEDYISGKFG"
     gene            4364..5017
                     /gene="phoU"
                     /locus_tag="VC122_001420"
     CDS             4364..5017
                     /gene="phoU"
                     /locus_tag="VC122_001420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294804.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate signaling complex protein PhoU"
                     /protein_id="HEP5641339.1"
                     /translation="MLRTKFEEELDKLHNQFYSMGTEVLAQINKTVRAFVSHDRELAK
                     EVIEEDDTINNFETKLEKKSLEIIALQQPVSNDLRMVITVLKASSDIERMGDHAASIA
                     KATIRMKGEERIPVVEEQINLMGKAVKQMVEEALNAYINADDTKAYEIAASDEIIDQY
                     FRNIQTLAVEEIRKSPDAVFAGKEYFQVLMYLERIGDYARNICEWIVYLKTGKIIEL"
     gene            5222..7759
                     /locus_tag="VC122_001421"
     CDS             5222..7759
                     /locus_tag="VC122_001421"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014612305.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M1 family metallopeptidase"
                     /protein_id="HEP5641340.1"
                     /translation="MKTVEHLIETFVPENYNIFLDINRQTKTFTGNVAINGEALDNHV
                     AFHQKDLDIKSILLDNEAVIYQVDNDNEVVRVELPETGMMTLVIEFSGSITDNMTGIY
                     PSYYTKNGEKKEVISTQFESHFAREAFPCIDEPQAKATFDLSLTFDQEIGEIALSNMP
                     EVNIDRREETGLWTFDTTPRMSSYLLAFALGELHGKTVESKKGTTVGVYATTAHPLSS
                     LDFSLDIAVRVINFYEDYFGVHYPIPQSLNIALPDFSSGAMENWGLITYREIYLLVDE
                     NSTVQSRQQVALVIAHEIAHQWFGNLVTMKWWDDLWLNESFANMMEYVSIEAIEPSWK
                     IIEDFQTGGIPLALKRDATDGVQSVHVEVNHPDEINTLFDPAIVYAKGSRLMHMLRRF
                     IGDRDFAIGLHHYFEKYQYRNTVGRDLWNILSDTSGKDVAAFMDAWLEQPGYPVLTAR
                     LENDQLILSQKQFFIGKGEEKGRLWPIPLNTNWHGLPETLTEAEMVIPNFSQLAAENE
                     GALRFNIDNTAHYITDYQGSLLDALVTELAQLDNTSALQVIQERRLLADSGLISYAEL
                     VDLIAQLDDSKSYMVAEAVQQVVSGLKRFIDEGSLAEKSFNRLVTTIYQEDFNQHGFE
                     KKADESDEDEMVRQVALGRLWLAENQTIIDGLRTIFEAYQNNIASIPAAVRRLVLANQ
                     MKYFETDSLVDIYFETYVATTDNNLRSDLTVAFSQTKQPTTIRRILVSLKDKDIIKPQ
                     DLSYWYNALLGQSFTQDIIWEWARENWDWIKSALGGDMSFDKFVIYPASNFKTPKHLA
                     EYKSFFEPKLDDMAISRNITMGINEIEARVALITKEKEAVIAALSHY"
BASE COUNT         2417 a         1395 c         1636 g         2521 t
ORIGIN      
        1 gaataggaaa taaataatga aaatgaaaaa aaaattcttt ttgttaagtc ttttggtcct
       61 atcaactttc tttttatccg catgttctag ctggattgat aaaggtgagt caataaccgc
      121 tgtaggatca acagcactac aacccttagt agaagcagta gctgatgaat ttggaagcag
      181 taatctaggc aagactgtca atgttcaagg tggtggttca ggtacagggt tgtctcaagt
      241 tcaatcagga gctgtccaaa ttggaaatag tgatgtcttt gcggaagaaa aagatggtat
      301 tgatgcttct aaattagttg atcatcaagt agctgttgca ggacttgcag ttattgccaa
      361 tcctaaagtc aaggtttcca atctcagtag tcagcagttg caaaagattt tttcaggaga
      421 atataccaat tggaaacaag ttggaggaga agatcttgcg atttcagtga tcaaccgagc
      481 agcaagttct ggctcacgag caacctttga cagtgttatc atgaaagggg tcaacgctaa
      541 acaaagtcaa gagcaagact ccaatgggat ggttaaatcg attgtttcac aaacaccagg
      601 tgccatttct tacctttcct ttgcctacgt tgattcatct gttaaatctt tgcaattaaa
      661 tgggtttaag gcaaatgcta agaacgtggc tacaaatgat tggccaatct ggtcctacga
      721 acacatgtat accaaagata aaccaacagg gttgaccaag gaatttcttg attatatgtt
      781 ttcagatgaa gtacaacaga acattgttac acatatggga tatatttcga taaatgatat
      841 ggaagtggtc aaatctcatg atggaaaagt aacaaaaagg taatggagga attatggaaa
      901 atcaggaatt ggctaaaaaa ttagcctcac catcaaagaa ttcaagactt gaaacctttg
      961 gccgcaccat tacgtttttg tgtctggctt tgattgtttt tatcgttgcg atgattctga
     1021 tcttcgttgc tcaaaaaggg ttgtcaacct tctttgttga caaggttaat ctttttgatt
     1081 tcttgtttgg aaaggagtgg caaccaagtg tgaaaaatgc ggccggcatt ccttatcttg
     1141 gtgctctgcc aatgattaca ggatcctttt tggttaccat tttatctgct attattgcca
     1201 ccccatttgc cattggcgca gccgttttta tgactgaaat ctcacctaaa tatggcgcta
     1261 aattattaca gcctgcggtt gagcttttgg ttggaattcc ttcggttgtt tatgggttta
     1321 tcggtttgca agtgattgtt ccttttatgc gctctatctt tggtggcaca ggttttggaa
     1381 tcctatctgg ggtctgtgtc ttgtttgtta tgattttacc aacagtgact tttatgacaa
     1441 cagacagtct gcgggcggtg cctcgtcatt accgcgaagc gtctatggct atgggagcaa
     1501 cctgttggca aaccatttgg cgtgttgttc ttaatgcagc tcgtccaggt atttttactg
     1561 ccattatttt cgggatggcg agagcttttg gtgaagcctt agccattcaa atggttgtcg
     1621 gtaactctgc tgtaatgcca agttcactaa cgacaccagc cgcaacctta acgtctgtct
     1681 tgacaatggg tattggtaac acggttatgg gaacggtcca aaataatgtg ctttggtccc
     1741 ttgccctagt cttattatta atgagtctgg ccttcaattc ccttgtcaaa ttaatcacga
     1801 aagaaagaaa gagaaattat gaacgctaaa aaagtcgata aagtagcaac tggtactctt
     1861 tataccattg ctggaattat tgtagctatt ttagcttcct taattctata tatccttgtc
     1921 cgtggcttgc cacacatcag ctggtccttt ttaacaggaa aatcgtcttc gtacgaagct
     1981 ggtgggggaa ttggtatcca gttgtataat tccttcttcc tattgattgt taccctgatt
     2041 atttccattc ctttatcaac tggagcgggg atttacttgg ctgaatatgc caaaaaaggg
     2101 cctgttacca actttattag aacctgtatt gagattctgt cttccctacc atctgtggtt
     2161 gtagggctct ttggttactt gattttcgtt gtgcagtttg aatatggctt ttctattatt
     2221 tcagggtctc ttgctttgac ggtctttaat cttcctcaaa tgacccgtaa tgttgaagat
     2281 agtttactgc atgttcatca tacccaaaga gaagcaggat tagctttagg cctgtcacgc
     2341 tgggaaacag ttttttatgt ggttatccca gaagctctcc cggggatggt aacaggtatt
     2401 gtcttagctt caggtcgtat ttttggtgaa gcggcagcgc ttatctatac tgctggtcaa
     2461 tcagcaccag ctctggattg gtcaaattgg aatccactta gtgttactag tccaatctct
     2521 attttccgtc aatctgagac ccttgctgtt catatttgga aagtcaatag cgaagggaca
     2581 attccagatg ctactcttgt atccgcagga agtgcagcag tgctattaat ttttatcctt
     2641 attttcaact tttcagctcg ctttattggg aagaaacttc attctaaaat gacagcagcg
     2701 aaataagata ttggagaaat catgacagaa tataattgga atgaacgtca tatcatcact
     2761 tttcctgaag aaacgcttgc tctagctacc aaggatttac atgtttacta tggtgcgaaa
     2821 gaagctatca aagggattga tatgcagttt gaaaaacata aaataacagc cttgattggt
     2881 ccatctggtt gtggcaaatc aacttatctt cgtagtctta accgcatgaa cgataccatt
     2941 gatattgctc gggttacagg tgagattctt tatcagggca ttgatgttaa tcgaaaagac
     3001 atgaatgttt acgagattcg caagcactta ggaatggttt ttcaaagacc caatcctttc
     3061 gctaaatcca tttataaaaa cattaccttt gcccatgaaa gagctggtgt gaaagacaaa
     3121 aaagttttag atgaaattgt tgagacttca ctgaaacaag ccgcgctttg ggatcaagtt
     3181 aaagatgacc ttcacaaatc agccttcacc ttatcagggg gtcagcaaca acgcttgtgt
     3241 attgctagag ccatttctgt caaacctgat attctattaa tggatgaacc agcctctgcc
     3301 cttgacccga ttgcaacgat gcagctggaa gaaaccatgt ttgaattgaa gaagaattac
     3361 acgattatta ttgtgaccca caatatgcaa caagcagctc gtgccagtga ttacactgcc
     3421 ttcttctatt taggcaacct cattgaatat gataagacac gtaatatttt ccaaaatgcg
     3481 caatgtcagt caacaaacga ctacgtttca gggcactttg gttagaaagg taatatgatg
     3541 acagaaccta ttttacagat cagagacctt tctgtttact acaatcaaaa gaaaacgtta
     3601 aaagatgtct ctttggacct ttatccaaat gaaataacag ctttaattgg tccttctgga
     3661 tctgggaaat caacgctctt gcgttctatc aaccgtatga atgatttgaa tcctgaagta
     3721 accattacag gctctatcgt ctataacgga cacaatattt atagtccaag gacagatacg
     3781 gttgatctac gcaaagaaat tggtatggta ttccaacaac ccaacccatt tccgatgtct
     3841 atttatgaga acgtggttta cggccttcgc ttaaaaggta tcagagataa atcaatctta
     3901 gaccatgctg ttgaaagctc tcttaaaggg gcttccattt ggaatgaagt caaagatcgt
     3961 ttacatgatt ctgcggttgg cttatcaggc ggacagcaac agagagtttg catagctaga
     4021 gttcttgcta ccagccccag aataatcctt ttagatgaac ctacctcagc tcttgatcct
     4081 atctcagctg ggaaaattga agaaacatta ttgttattaa aaaaagatta tacactggct
     4141 attgtgaccc gctcaatgca gcaagcctca cgtttatcag atcgaacagg ttttttcttg
     4201 gagggtgact tacttgagtg tggtcctaca aaagctatgt ttatgaatcc taagcgcaaa
     4261 gaaaccgaag actatatttc tggtaaattt ggttgagaaa gacgtatatg gttgacccct
     4321 ctgtgttttt attataatga ctagattgta ggaagaaaaa atcatgttaa gaacgaaatt
     4381 tgaagaagaa ttagataaat tgcataacca gttttattca atgggaacgg aagtattggc
     4441 acaaatcaat aaaactgttc gtgcctttgt tagtcatgat cgtgaattgg ccaaagaagt
     4501 tatcgaagaa gatgacacta ttaataattt tgagacaaag cttgaaaaga aatcgctgga
     4561 aattattgcc ctacaacagc cagtttcgaa tgatcttaga atggttatta ctgttttaaa
     4621 ggcatcaagt gatattgaac gtatgggtga ccatgctgct tcgattgcca aggccactat
     4681 tcgtatgaaa ggtgaggagc gtattccagt ggttgaagaa caaatcaacc ttatgggaaa
     4741 agctgtgaaa cagatggtag aagaagccct taatgcatat atcaatgctg acgatacaaa
     4801 agcttatgaa atcgcagctt ctgatgagat tatcgatcaa tatttccgta atatccaaac
     4861 cttagctgtt gaagaaatcc gtaagtctcc agatgctgtt tttgcaggaa aagaatattt
     4921 tcaagtatta atgtatttgg aacgtattgg tgactatgct cgcaacattt gtgaatggat
     4981 tgtttatctt aaaactggaa aaattattga attataagaa attatgctat aatgagactg
     5041 ggaaacagct tgccagtccc tggttttata cgaaattgtg caagttagaa aatgaactaa
     5101 caagaaaagt tttctttgct gaatctgatt ttgttaggtt aacaaatata tgtagacaga
     5161 gatgatactt aggtatcatc tgttgatcta ttatgatttg ttattagaat taaggagtta
     5221 tatgaagaca gtagaacatc tcatcgaaac atttgtgcct gaaaattata acatcttttt
     5281 agatattaat cgtcagacta aaacatttac ggggaatgtt gcgattaatg gtgaagcttt
     5341 agataatcat gtggctttcc accaaaaaga tttggacatt aagtctattt tgctcgataa
     5401 tgaagctgtt atttatcaag tggataacga caatgaagtt gtgcgtgttg aattacctga
     5461 aacaggaatg atgacgcttg tcattgaatt ttcaggtagc attacagata atatgacagg
     5521 tatttaccct tcatattaca ctaaaaatgg tgagaaaaaa gaagtcattt caactcagtt
     5581 tgaaagccat tttgcacgtg aagctttccc atgtattgat gaaccacaag caaaagctac
     5641 ttttgattta agcttgacct ttgatcaaga aattggtgag atagcgttat cgaatatgcc
     5701 agaagtcaat attgaccgtc gcgaggagac tggtctttgg acatttgata cgacaccaag
     5761 aatgtcatct tacttgctag cgtttgcgct aggagaactt catggaaaga ctgtagaaag
     5821 caaaaaagga acaactgttg gtgtgtatgc gacaacagcg catcctctta gctcgcttga
     5881 tttttcattg gatattgcag ttcgtgttat caacttttat gaagactatt ttggagtaca
     5941 ttacccaatc ccgcaatctc ttaatattgc gcttccagat ttttcatctg gtgctatgga
     6001 aaattggggt ttaatcactt atcgtgaaat ttatttatta gtagatgaaa attcgactgt
     6061 tcaaagccga caacaagttg cacttgtaat cgcccacgag atagcacacc aatggtttgg
     6121 aaacttggtt acaatgaaat ggtgggacga tctgtggtta aatgagagtt ttgccaacat
     6181 gatggaatat gtctctatcg aggctattga gccgtcttgg aaaataatag aagatttcca
     6241 aacaggtggt attcctcttg ctttaaaacg tgatgctact gatggtgttc aatcagtgca
     6301 tgttgaagtc aatcatccag atgagataaa tacattgttt gatcctgcta ttgtctatgc
     6361 aaaaggtagt cgtttgatgc atatgttacg ccgttttatt ggtgatcgtg attttgccat
     6421 tggcttacat cactactttg aaaaatatca atatcgaaat actgtcgggc gtgatttatg
     6481 gaatatttta tcagatactt caggcaaaga cgtagctgct tttatggatg cttggcttga
     6541 acaaccaggt tatccagttt taacagctcg ccttgaaaac gatcaattga ttttgagtca
     6601 aaaacaattt tttattggaa aaggtgaaga aaaaggtcgc ctttggccga ttcctttaaa
     6661 cactaattgg catggccttc ctgaaacttt gacagaggca gaaatggtca ttccaaactt
     6721 tagtcaattg gcagcagaaa atgaaggagc tctacgtttc aatattgata atacagctca
     6781 ttacattaca gactaccaag gatcattatt agatgcctta gtcactgaac ttgcccaact
     6841 tgataatact tctgctttac aagttattca agaacgtcgt ttattggcag atagtggctt
     6901 gatttcctat gctgaattgg ttgacttaat tgctcaattg gacgattcta agtcttatat
     6961 ggtagcagaa gcggttcagc aagtggtctc tggtttgaaa cgcttcattg atgaaggaag
     7021 cttagcagaa aaatccttta atcgcttggt aaccaccatc tatcaagaag actttaatca
     7081 gcatggtttt gagaaaaaag ctgacgaatc agatgaagat gagatggtac gtcaggttgc
     7141 cttgggccgc ttgtggctag cagaaaatca gacgattatt gacggattga gaactatttt
     7201 tgaagcatat caaaacaata ttgcaagtat tccagcagct gttcgtcgct tggttttagc
     7261 caaccaaatg aaatattttg aaacagatag cttggttgat atttattttg agacttatgt
     7321 agcaactact gataataatt tacgcagtga tttaacggtt gcttttagtc aaacaaaaca
     7381 accaactact attagacgta ttcttgttag tttaaaagat aaagatatta ttaaacctca
     7441 ggatttatct tattggtaca atgctttgct tggacaaagc tttactcaag atatcatttg
     7501 ggaatgggct agagaaaatt gggattggat taaatctgca cttggtggag atatgagctt
     7561 tgataaattt gttatttatc cagcaagtaa ctttaaaaca ccaaaacatc tagcagaata
     7621 taaatctttc tttgaaccta agctagatga tatggctatt agccgtaata ttaccatggg
     7681 aattaatgaa attgaagcac gtgttgcttt gatcactaaa gaaaaagaag cagttattgc
     7741 agctctcagt cattactaga ccataagcaa aagagttggg aggactttcc agctcttttt
     7801 tgtagtgagc tagcttcctt tcttagttcc gtctataact aaatgattat ccatagttta
     7861 attcaaaagg agattgcact ttaaagcgaa ggctattagc ctcttaatac tttttttacc
     7921 aggtggtgaa taagaacaat tataaatgat ttaatcatta tgctttaaa
//
LOCUS       DATXIG010000057         7930 bp    DNA     linear   BCT 28-DEC-2023
DEFINITION  TPA_asm: Streptococcus pyogenes strain MGAS11113
            SAMN08555799-rid19682583.denovo.057, whole genome shotgun sequence.
ACCESSION   DATXIG010000057 DATXIG010000000
VERSION     DATXIG010000057.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN08555799
            Sequence Read Archive: SRR6740495
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pyogenes
  ORGANISM  Streptococcus pyogenes
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 7930)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7930)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-OCT-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-OCT-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001918058.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001918058.2
            Genome Coverage       :: 368x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/12/2023 11:23:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 1,806
            CDSs (total)                      :: 1,769
            Genes (coding)                    :: 1,722
            CDSs (with protein)               :: 1,722
            Genes (RNA)                       :: 37
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1, 1 (5S, 23S)
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 47
            CDSs (without protein)            :: 47
            Pseudo Genes (ambiguous residues) :: 0 of 47
            Pseudo Genes (frameshifted)       :: 30 of 47
            Pseudo Genes (incomplete)         :: 20 of 47
            Pseudo Genes (internal stop)      :: 9 of 47
            Pseudo Genes (multiple problems)  :: 11 of 47
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7930
                     /organism="Streptococcus pyogenes"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN08555799-rid19682583.denovo.057"
                     /strain="MGAS11113"
                     /serotype="Emm28"
                     /isolation_source="normally sterile site"
                     /host="Homo sapiens"
                     /db_xref="taxon:1314"
                     /country="Finland"
                     /collection_date="2002"
                     /collected_by="National Public Health Laboratory"
     gene            complement(<1..697)
                     /locus_tag="VC122_001422"
     CDS             complement(<1..697)
                     /locus_tag="VC122_001422"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002989610.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEP5641341.1"
                     /translation="MFGKLLKYEFRSIGKWYFALNAFVIAIAAILSFTIKLFAQSNSD
                     GLFGVLTNKMLPLTLGLTFGSLIAGSLLSTLLIIIKRFSKSVFGREGYLTLTLPVNSH
                     QIILSKLLASFICSVFNTIILAFAIAIVIVPMFNINELLEGFFNSFKMDYFINMLTVL
                     AYVLLSTFTSILLIYLSISIGQLFSNRRGLMAFIAYFILVILISVAATYVHSHIFNIN
                     TSADSFPFTEQKTI"
     gene            complement(707..1405)
                     /locus_tag="VC122_001423"
     CDS             complement(707..1405)
                     /locus_tag="VC122_001423"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002984437.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEP5641342.1"
                     /translation="MAHLLQLHHVSKSYREKKAIDDLTITIPNGKIIGLLGPNGSGKT
                     TLIKLINGLLQPNKGEIVIDGYRPCVETKKIISYLPDTTYLNENMRIKDMLEFFSDFY
                     SDFDKSKATSLLRDLELDPEDRFKTLSKGNKEKVQLILVMSRKARLYVLDEPIGGVDP
                     AARDYILKTIINSYCENASVIISTHLISDIEPILDEVIFLKQGRLFLSGNADDLRQEY
                     QQSIDSLFRETYKV"
     gene            complement(1405..1776)
                     /locus_tag="VC122_001424"
     CDS             complement(1405..1776)
                     /locus_tag="VC122_001424"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010922377.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HEP5641343.1"
                     /translation="MSWKFDEKSPIYAQIAQHVMMQIISQEIKSGDQLPTVREYAEIA
                     GVNPNTMQRAFTELEREGMVYSQRTAGRFVTDDQKLIARKRRELAISELESFITNMTK
                     MGFSHTEIIPVLTSFLKEDSQ"
     gene            1961..5071
                     /locus_tag="VC122_001425"
     CDS             1961..5071
                     /locus_tag="VC122_001425"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011017834.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit alpha"
                     /protein_id="HEP5641344.1"
                     /translation="MFAQLDTKTVYSFMDSLIDLNHYFERAKQFGYHTIGIMDKDNLY
                     GAYHFIKGCQKNGLQPVLGLEVEILYQERQVLLNLIAQNTQGYHQLLKISTAKMSGKL
                     HMDYLCQHLEGIAVIIPSKGWSDTLVVPFDYYIGVDQYTDLSHMDSKRQLIPLRTVRY
                     FAQDDMETLHMLHAIRDNLSLAETPVVESDQELADCQQLTTFYQTHCPQALQNLEDLV
                     SGIYYDFDTNLKLPHFNRDKSAKQELQDLTEAGLKEKGLWKEPYQSRLLRELVIISDM
                     GFDDYFLIVWDLLRFGRSKGYYMGMGRGSAAGSLVAYALNITGIDPVQHDLLFERFLN
                     KERYSMPDIDIDLPDIYRSEFLRYVRNRYGSDHSAQIVTFSTFGPKQAIRDVFKRFGV
                     PEYELTNLTKKIGFKDSLATVYEKSISFRQVINSRTEFQKAFAIAKRIEGNPRQTSIH
                     AAGIVMSDDTLTNHIPLKSGDDMMITQYDAHAVEANGLLKMDFLGLRNLTFVQKMQEK
                     VAKDYGCQIDIAAIDLEDPQTLALFAKGDTKGIFQFEQNGAINLLKRIKPQRFEEIVA
                     TTSLNRPGASDYTTNFIKRREGQEKIDLIDPVIAPILEPTYGIMLYQEQVMQIAQIYA
                     GFTLGKADLLRRAMSKKNLQEMQKMEEDFIASAKHLGRAEETARGLFKRMEKFAGYGF
                     NRSHAFAYSALAFQLAYFKAHYPAVFYDIMMNYSSSNYITDALESDFQVAQVTINSIP
                     YTDKIEASKIYMGLKNIKGLPRDFAYWIVEQRPFNSVEDFLTRTPEKYQKKVFLEPLI
                     KIGLFDCFEPNRKKIMDNLDGLLVFVNELGSLFSDSSFSWVDTKDYSATEKYSLEQEI
                     VGVGMSKHPLIDIAEKSTQTFTPISQLVKESEAVVLIQIDSIRIIRTKTSGQQMAFLS
                     VNDTKKKLDVTLFPQEYAIYKDQLKEGEFYYLKGRIKERDHRLQMVCQQVQMAISQKY
                     WLLVENHQFDSQISEILGAFPGTTPVVIHYQKNKETIALTKIQVHVTENLKEKLRPFV
                     LKTVFR"
     gene            5151..6164
                     /gene="pfkA"
                     /locus_tag="VC122_001426"
     CDS             5151..6164
                     /gene="pfkA"
                     /locus_tag="VC122_001426"
                     /EC_number="2.7.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012678045.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6-phosphofructokinase"
                     /protein_id="HEP5641345.1"
                     /translation="MKRIAVLTSGGDAPGMNAAIRAVVRKAISEGMEVYGINRGYAGM
                     VDGDIFPLGSKEVGDKISRGGTFLYSARYPEFAQLEGQLAGIEQLKKHGIEGVVVIGG
                     DGSYHGAMRLTEHGFPAVGIPGTIDNDIAGTDYTIGFDTAVNTAVEAIDKLRDTSSSH
                     GRTFVVEVMGRNAGDIALWAGIASGADQIIVPEEEFDIEKVASTIQYDFEHKGKNHHI
                     IVLAEGVMSGEAFAQKLKEAGDKSDLRVTNLGHILRGGSPTARDRVIASWMGSHAVEL
                     LKEGKGGLAVGIHNEELVESPILGTAEEGALFSLTEEGKIIVNNPHKARLDFAALNRS
                     LSQ"
     gene            6227..7729
                     /gene="pyk"
                     /locus_tag="VC122_001427"
     CDS             6227..7729
                     /gene="pyk"
                     /locus_tag="VC122_001427"
                     /EC_number="2.7.1.40"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018366040.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate kinase"
                     /protein_id="HEP5641346.1"
                     /translation="MNKRVKIVATLGPAVEIRGGKKYGEDGYWAGQLDVEESAKKIAE
                     LIEAGANVFRFNFSHGDHKEQGDRMATVRLAEEIARQKVGFLLDTKGPEMRTELFADD
                     AKEFSYVTGEKIRVATTQGIQSTRDVIALNVAGSLDIYDEVEVGHTILIDDGKLGLKV
                     IDKDIATRQFIVEVENDGIIAKQKGVNIPNTKIPFPALAERDNADIRFGLEQGLNFIA
                     ISFVRTAKDVEEVREICCETGNDHVQLFAKIENQQGIDNLDEIIEAADGIMIARGDMG
                     IEVPFEMVPVFQKMIITKVNAAGKAVITATNMLETMTEKPRATRSEVSDVFNAVIDGT
                     DATMLSGESANGKYPVESVRTMATIDRNAQTLLNEYGRLDSSAFPRTNKTDVIASAVK
                     DATHSMDIKLVVTITETGNTARAISKFRPDADILAVTFDEKVQRALMINWGVIPVLAE
                     KPASTDDMFEVAERVAVEAGLVQSGDNIVIVAGVPVGTGGTNTMRVRTVK"
BASE COUNT         2511 a         1336 c         1641 g         2442 t
ORIGIN      
        1 aaattgtttt ttgctcagta aatgggaaac tatcggcact tgtattaata ttaaagatgt
       61 gactgtgaac atatgttgca gcaacgctaa tcagaataac taatataaaa tatgcaataa
      121 aggccatcaa gcctcgccga ttagaaaaaa gttgacctat agaaatggaa agataaatta
      181 ataagatact cgtaaatgtt gataatagga cataggctag tacagttagc atattgatga
      241 aataatccat cttaaaacta ttaaagaatc cttctaatag ttcgttgatg ttaaacattg
      301 gtacaattac aatagcgata gcaaatgcaa ggatgatagt attgaaaaca ctgcaaataa
      361 aagaagctag tagttttgat aaaataattt gatgcgaatt aacgggtaaa gtcaacgtta
      421 agtatccttc ccgtccaaaa acacttttgc taaaacgttt gataatgata agtaatgttg
      481 ataagaggga accagcgatc agggagccaa aagttaaacc taatgtcaga ggtaacatct
      541 tattggttag tactccaaat agtccatcac tattactttg agcaaacagt tttatcgtaa
      601 atgacaaaat ggcagcgatg gcaataacga aagcattaag cgcaaagtac cattttccaa
      661 tagatctaaa ttcgtatttt aataattttc caaacatgat atcctcctaa accttataag
      721 tttccctaaa gagggaatca atggattgct ggtattcttg tcttagatca tcagcattgc
      781 cagataagaa aagtcttcct tgtttcaaga aaatgacttc gtcaaggata ggttcaatgt
      841 ccgaaatcaa gtgggttgag ataataacag aagcattttc acaataacta ttaatgatgg
      901 tttttaggat gtagtctctt gcggcagggt caactccacc aataggttca tcaagtacat
      961 agagtctggc tttacgactc atcacaagaa ttaattgtac tttttctttg tttcctttgg
     1021 atagagtttt gaagcggtct tcagggtcta gttctaaatc tcttagaagg cttgttgctt
     1081 tggatttgtc aaagtcgctg taaaaatcac taaagaactc caacatgtcc ttaattctca
     1141 tattttcatt gagataagta gtatctggta aataagaaat aatttttttt gtctcaacac
     1201 agggacggta gccatcaatg acaatttctc ctttatttgg ttgtaaaagg ccattaatga
     1261 gtttaattaa agttgtttta ccacttccat ttggacctaa taatccgata attttaccgt
     1321 tcgggatagt aatggttaag tcatcaatag cttttttttc acggtaagat tttgaaacat
     1381 gatgtagttg caataggtgt gccattattg tgaatcctcc tttaaaaagc tcgtcaaaac
     1441 agggataatc tcagtgtgac taaatcccat ttttgtcata ttggtgataa atgattctaa
     1501 ttcactaatt gctaactctc gtcttttacg agcaattaat ttttgatcgt cagttacaaa
     1561 acggccagca gtacgttgcg aataaaccat tccttcccgt tcaagttctg taaacgctcg
     1621 ttgcatggtg ttaggattaa cacctgcgat ttctgcatat tcacgaacag ttggaagttg
     1681 atcaccactc ttgatttctt ggctaataat ttgcatcatg acatgttgtg ctatctgcgc
     1741 ataaatagga gatttttcat caaatttcca agacatgtgg tctcctttct gagtaatata
     1801 gatatttgta ctaattaaat ggtacagaaa aaaaaaggga atgtcaagtt ttatgccatt
     1861 tttcactctt tgagaagtag acgtagatta gagagaaaat tgaagcaaga aaaccttttt
     1921 catagtataa tagggaacaa gataaccaaa ggaggaactt atgtttgctc aacttgatac
     1981 taaaactgta tactcattta tggatagttt aattgactta aatcattatt ttgaacgagc
     2041 aaagcaattt ggttaccaca ccataggaat catggataag gataatcttt atggtgctta
     2101 ccattttatt aaaggttgtc aaaaaaatgg actgcagcca gttttaggtt tggaagtaga
     2161 gattctctat caagagcggc aggtgctcct taacttaatc gcccagaata cacaaggcta
     2221 tcatcagctt ttaaaaattt ccacggcaaa aatgtctggc aagcttcata tggattacct
     2281 ctgccaacat ttggaaggga tagcggttat tattcctagt aagggttgga gcgatacatt
     2341 agtggtccct tttgactact atatcggtgt tgatcagtat actgatttat ctcatatgga
     2401 ttctaagagg cagcttatac ccctaaggac agttcgttat tttgcgcaag atgatatgga
     2461 aaccttgcac atgttgcatg ccattcgaga taacctcagt ctggcagaga cccctgtggt
     2521 agaaagtgat caagagttag cagattgtca acaactaacc accttctatc aaacacactg
     2581 ccctcaagct ctacagaatt tagaagactt agtgtcagga atctattatg atttcgatac
     2641 aaatttaaaa ttgccacatt ttaatagaga taagtctgcc aagcaagaat tgcaagactt
     2701 gactgaggct ggtttgaagg aaaaaggatt gtggaaagag ccttatcaat cgcgcttact
     2761 gcgtgaattg gtcattattt ctgacatggg ctttgatgat tattttttga ttgtgtggga
     2821 tttacttcgc tttggacgca gtaaaggcta ttatatgggt atgggacgtg gctcggcggc
     2881 aggtagtcta gtggcttatg ctctgaacat tacagggatt gatccagttc aacatgattt
     2941 gctatttgag cgctttttaa acaaagaacg ttatagcatg cctgatattg atatcgatct
     3001 tccagatatt taccgttcag aatttctacg gtatgtccga aatcgttatg gtagcgacca
     3061 ttcggcgcaa attgtgacct tttcaacctt tggccctaaa caggctattc gtgatgtttt
     3121 caaacggttc ggggttccag aatacgaact gactaatctc actaaaaaaa ttggttttaa
     3181 agatagcttg gctactgtct atgaaaagtc aatctctttt aggcaggtta ttaatagtag
     3241 aactgaattt caaaaggcct ttgctattgc caagcgtatc gaaggaaatc caagacaaac
     3301 gtccattcac gcagctggta ttgtgatgag tgatgatacc ttgaccaatc atattcctct
     3361 aaaatcgggc gatgacatga tgatcaccca gtatgatgct catgcggtcg aagctaatgg
     3421 cctgttaaaa atggattttt tggggttaag aaatttgacc tttgttcaaa aaatgcaaga
     3481 gaaggttgct aaagactacg ggtgtcagat cgatattgca gccattgatt tagaagaccc
     3541 gcaaacgttg gcactttttg ctaaagggga taccaaggga attttccaat ttgaacaaaa
     3601 tggtgctatt aatcttttaa aacggattaa gccacaacgt tttgaagaaa ttgttgccac
     3661 taccagtcta aatagaccag gggcaagtga ctataccact aatttcatta aacgaagaga
     3721 aggacaagaa aaaattgatt tgattgatcc tgtgattgct cccattttag agccaactta
     3781 cggtattatg ctttatcaag aacaagttat gcagattgca cagatttatg ctggttttac
     3841 gttaggcaag gccgacttgt taaggcgtgc catgtctaaa aaaaatctac aagaaatgca
     3901 aaaaatggaa gaagacttta ttgcttctgc taagcaccta gggagagctg aagaaacagc
     3961 tagaggactt tttaaacgga tggaaaaatt tgcaggttat ggttttaacc gcagccatgc
     4021 ctttgcctat tcagctttag cttttcaatt ggcttatttc aaagcccatt acccggctgt
     4081 tttttacgat atcatgatga attattctag cagtaactat atcacagatg ctctagaatc
     4141 agattttcaa gtagcgcaag ttaccattaa tagtattcct tacactgata aaattgaagc
     4201 tagcaagatt tacatggggc tgaaaaatat taaggggttg ccaagggatt ttgcttattg
     4261 gattgttgag caaagaccat ttaatagcgt agaggatttt ctcactagaa ctccagaaaa
     4321 atatcaaaaa aaggttttcc ttgagcctct gataaaaata ggtctgtttg attgctttga
     4381 gcctaaccgt aaaaaaatta tggacaattt ggatggttta ctggtatttg ttaatgagct
     4441 tggttctctt ttttcagatt cgtcctttag ttgggtagat acgaaagatt actcagcaac
     4501 tgaaaaatat tctttggaac aggagatcgt tggggttggc atgagcaagc atcctttaat
     4561 tgatattgct gagaaaagta cccaaacttt tactcccatt tcacagttag tcaaagaaag
     4621 cgaagcagtc gtactgattc aaatagatag cattaggatc attagaacca aaacaagtgg
     4681 gcagcaaatg gcttttttaa gtgtgaatga cactaagaaa aagctcgatg tcacactttt
     4741 tccacaagag tatgctattt ataaagacca attaaaagaa ggagaattct attacttaaa
     4801 aggtagaata aaagaaagag accatcgact gcagatggtg tgtcagcaag tgcaaatggc
     4861 tattagtcaa aaatattggt tattagttga aaaccatcag tttgattccc aaatttctga
     4921 gattttaggt gcctttccag gaacgactcc agttgttatt cactatcaaa aaaataagga
     4981 aacaattgca ttaactaaga ttcaggttca tgtaacagag aatttaaagg aaaaacttcg
     5041 tccttttgtt ctgaaaacgg tttttcgata aaaatggaca attagtgaag tattttgcaa
     5101 atgaatgtgc tataataaga ccgttaaatt aattaaagga gtaccagaaa atgaaacgta
     5161 ttgctgtttt aaccagtggc ggcgacgctc ctggtatgaa cgctgctatt cgtgcagtcg
     5221 ttcgtaaagc aatttcagaa ggaatggaag tttatggtat caaccgtggc tatgcaggta
     5281 tggttgatgg cgacattttc ccactgggtt caaaagaagt tggtgataaa atctcacgtg
     5341 gtggaacttt cttatactca gctcgttatc cagagtttgc tcaacttgaa ggtcaattgg
     5401 ctggtattga gcaacttaaa aaacatggta ttgaaggtgt tgttgttatt ggtggggatg
     5461 gttcttatca tggcgctatg cgcttgacag agcacggctt cccagcggta ggtatcccag
     5521 gaacaatcga taacgatatt gcgggaactg actacactat cggatttgat acagcagtta
     5581 acacagctgt tgaagctatt gataaacttc gtgacacctc atcaagtcac ggacgtactt
     5641 ttgtggttga agtgatgggt cgcaatgcgg gtgatattgc tctttgggca ggtattgcta
     5701 gtggtgctga tcaaatcatt gttccagaag aagagtttga tattgaaaaa gtggcatcaa
     5761 caatccaata tgattttgaa cacaaaggta aaaatcacca cattatcgtc ttagctgaag
     5821 gtgttatgag cggtgaagct tttgctcaaa aactgaaaga agctggcgat aagagtgacc
     5881 ttcgtgtgac aaatcttggc cacattcttc gtgggggttc accaactgct cgtgaccgtg
     5941 tcattgcttc ttggatgggt tcacatgctg ttgagctact taaagaaggc aaaggtggtt
     6001 tggctgttgg tattcacaat gaagaacttg ttgaaagccc aatccttggt actgcagaag
     6061 aaggtgcttt gttcagcttg actgaagaag gcaagattat tgttaataat ccacacaaag
     6121 ctcgtttaga ctttgcagct ttgaaccgtt cattgtcaca ataagtttag ttaaaattgt
     6181 taaatcgtca attattgaca aaaaaataag ggagtttcag agtaaaatga ataaacgcgt
     6241 aaaaatcgtt gcaacacttg gtcctgcggt tgaaatccgt ggtggtaaaa aatacggaga
     6301 agatggctac tgggctggtc aacttgatgt tgaagaatct gcaaaaaaaa tcgctgaatt
     6361 aattgaagct ggtgcgaatg ttttccgttt caatttctca catggtgacc acaaagaaca
     6421 aggtgaccgt atggcaactg tccgccttgc agaagaaatt gctcgtcaaa aagttggttt
     6481 ccttttggat actaaaggtc ctgaaatgcg tacggaattg tttgcagatg atgctaaaga
     6541 gttcagctat gttactggag aaaaaatccg tgtagcgact acacaaggta ttcaatcaac
     6601 tcgtgatgtt attgctttga acgttgctgg tagccttgac atctacgatg aagtagaagt
     6661 aggtcatact attcttatcg atgatggtaa acttggtttg aaagtgatcg acaaagatat
     6721 cgcaacacgt caatttatcg ttgaagtaga aaacgacggt attattgcta aacaaaaagg
     6781 tgttaatatc cctaacacta aaattccttt cccagcactt gcggaacgcg acaatgctga
     6841 tatccgtttt ggtcttgaac aaggacttaa ctttattgca atctcatttg ttcgtacagc
     6901 caaagacgtt gaggaagttc gtgaaatctg ttgcgaaact ggtaacgacc atgtgcaatt
     6961 atttgctaag attgaaaacc aacaaggtat cgataatctt gatgaaatca tcgaagctgc
     7021 tgatggtatc atgattgcac gtggcgacat gggtatcgaa gtaccatttg aaatggttcc
     7081 agttttccaa aaaatgatta tcactaaagt gaatgcagct ggtaaagcag ttatcacagc
     7141 aacaaacatg cttgaaacaa tgactgaaaa accacgtgcg acacgttctg aagtatctga
     7201 cgtcttcaat gctgttatcg atggtactga tgcaacaatg ctttcaggag agtcagctaa
     7261 cggtaaatac ccggttgaat ctgttcgtac aatggcgact attgatcgta atgcacaaac
     7321 tcttcttaac gaatatggtc gtttagattc atctgccttt ccacgtacca ataaaacaga
     7381 tgttattgct tcagctgtta aagatgccac tcattcaatg gacatcaaac ttgttgtgac
     7441 tattactgag acaggtaata cagctcgtgc gatttctaaa ttccgtccag atgcggacat
     7501 cttagctgta acatttgatg aaaaagtaca acgtgctttg atgattaact ggggtgttat
     7561 tcctgttctt gctgaaaaac ctgcatctac tgatgacatg tttgaagtag cagagcgtgt
     7621 tgctgtggag gcaggcttag ttcaatctgg ggataatatt gttattgttg ctggtgttcc
     7681 agttggtact ggtggaacga atactatgcg tgttcgtaca gtaaaataaa tgtgttataa
     7741 ctatttacct aaaggttaga aggcaagctt ctaacctttt ttgatatcaa aaatatcatg
     7801 ttaattcact agtgttaatt tactttaatg agtctaagtt tgtattttat acagaatgag
     7861 aaacgtttat tggtattgat caaaaattgt ttactttttt tctagcttag aacatggtat
     7921 aataagacaa
//
LOCUS       DATXIG010000058         7884 bp    DNA     linear   BCT 28-DEC-2023
DEFINITION  TPA_asm: Streptococcus pyogenes strain MGAS11113
            SAMN08555799-rid19682583.denovo.058, whole genome shotgun sequence.
ACCESSION   DATXIG010000058 DATXIG010000000
VERSION     DATXIG010000058.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN08555799
            Sequence Read Archive: SRR6740495
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pyogenes
  ORGANISM  Streptococcus pyogenes
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 7884)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7884)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-OCT-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-OCT-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001918058.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001918058.2
            Genome Coverage       :: 368x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/12/2023 11:23:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 1,806
            CDSs (total)                      :: 1,769
            Genes (coding)                    :: 1,722
            CDSs (with protein)               :: 1,722
            Genes (RNA)                       :: 37
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1, 1 (5S, 23S)
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 47
            CDSs (without protein)            :: 47
            Pseudo Genes (ambiguous residues) :: 0 of 47
            Pseudo Genes (frameshifted)       :: 30 of 47
            Pseudo Genes (incomplete)         :: 20 of 47
            Pseudo Genes (internal stop)      :: 9 of 47
            Pseudo Genes (multiple problems)  :: 11 of 47
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7884
                     /organism="Streptococcus pyogenes"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN08555799-rid19682583.denovo.058"
                     /strain="MGAS11113"
                     /serotype="Emm28"
                     /isolation_source="normally sterile site"
                     /host="Homo sapiens"
                     /db_xref="taxon:1314"
                     /country="Finland"
                     /collection_date="2002"
                     /collected_by="National Public Health Laboratory"
     gene            complement(<1..641)
                     /gene="speG"
                     /locus_tag="VC122_001428"
     CDS             complement(<1..641)
                     /gene="speG"
                     /locus_tag="VC122_001428"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010921857.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="streptococcal pyrogenic exotoxin SpeG"
                     /protein_id="HEP5641347.1"
                     /translation="MKTNILTIIILSCVFSYGSQLAYADENLKDLKRSLRFAYNITPC
                     DYENVEIAFVTTNSIHINTKQKRSECILYVDSIVSLGITDQFIKGDKVDVFGLPYNFS
                     PPYVDNIYGGIVKHSNQGNKSLQFVGILNQDGKETYLPSEAVRIKKKQFTLQEFDFKI
                     RKFLMEKYNIYDSESRYTSGSLFLATKDSKHYEVDLFNKDDKLLSRDSFFKRY"
     gene            complement(743..841)
                     /gene="ffs"
                     /locus_tag="VC122_001429"
     ncRNA           complement(743..841)
                     /ncRNA_class="SRP_RNA"
                     /gene="ffs"
                     /locus_tag="VC122_001429"
                     /product="signal recognition particle sRNA small type"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00169"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00169"
     gene            1041..2273
                     /locus_tag="VC122_001430"
     CDS             1041..2273
                     /locus_tag="VC122_001430"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011017300.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S-layer protein"
                     /protein_id="HEP5641348.1"
                     /translation="MGVMMKQKIKILTVIGLMTVGMSACHNTSKPSNTDSVFSLTGKK
                     RQQIVKQVRQRYYFQQLSKTEQENYLTLYDSLAQFREIISLTPASKKSLIKTIDAFVM
                     DNPEFYWITSADYRFEFSDQTVFVTFPIPEDAKNVYQDLQAIGNDIVANTPSKDRYEQ
                     VKYFYEVIIRDTDYNKKAFEAYQSGSQAQVASNQDIKSVFIDHLSVCNGYAQAFQFLC
                     QKAGIPVAYIRGTGTSQQPQQSFAHAWNAVQINNTYYGVDVTWGDPVFDNHLSHQKQG
                     TINYSFLCLPDYLMALSHQPSKDIAFNTKERFENVWTIPSCTDDSLLYSKRHQSYIST
                     FDSDAILASLENQLLNRQEQLCLQFAHQDDYQQMVTDLTTNQTGYHNLFNQYWNNYTG
                     FTYGLLPETLSISFASRN"
     gene            complement(2887..3402)
                     /locus_tag="VC122_001431"
     CDS             complement(2887..3402)
                     /locus_tag="VC122_001431"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011285373.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside deaminase"
                     /protein_id="HEP5641349.1"
                     /translation="MPYSLEEQTYFMQEALKEAEKSLQKAEIPIGCVIVKDGEIIGRG
                     HNAREESNQAIMHAEMMAINEANAHEGNWRLLDTTLFVTIEPCVMCSGAIGLARIPHV
                     IYGASNQKFGGADSLYQILTDERLNHRVQVERGLLAADCANIMQTFFRQGRERKKIAK
                     HLIKEQSDPFD"
     gene            complement(3402..4181)
                     /locus_tag="VC122_001432"
     CDS             complement(3402..4181)
                     /locus_tag="VC122_001432"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012560420.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HEP5641350.1"
                     /translation="MKLTTLGSWGAYPYQDAGTTSYLVTGHDGFQLLMDAGSRALNEL
                     EKEISPLDLDAVIISHYHPDHVADLGVLRHYFQLYPKHLWQPKVLPIYGHDEDRHEFA
                     KLSIPDVSEGRAYDVTGVEQIGPFDITFLKTVHPVVCYAFRIVERATGQVLVFTGDSG
                     YFEGLADFAKEADLFLADVYLYEGNENHMAHLTSKEAGLMASQAGVKKLVLTHMPPVP
                     PEGIDPDNHLEVLRQETQAYAGNIPVDLALPHKSWNLGSLS"
     gene            complement(4324..4860)
                     /locus_tag="VC122_001433"
     CDS             complement(4324..4860)
                     /locus_tag="VC122_001433"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002986314.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="biotin transporter BioY"
                     /protein_id="HEP5641351.1"
                     /translation="MFTTKELVKVAMMTTLIIILGFIPAIPLGFIPVPIVLQNLGVML
                     AGLMLGGKKGTLSVFLFLVIGLFLPVFSGSRTTIPVLMGPSAGYVIAYLLVPIVFSLL
                     YRNWFSKSTPLAFLALLISGVVLVDVLGAIWLAAYTGMSLVTSLLSNLVFIPGDTIKA
                     IIATIIAVKYKDSFLNTK"
     gene            complement(4864..5175)
                     /locus_tag="VC122_001434"
     CDS             complement(4864..5175)
                     /locus_tag="VC122_001434"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003045539.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEP5641352.1"
                     /translation="MTDCFGIDLDQEYRCLHYHTPLDIVGLKCAFCQTYYACYHCHDQ
                     LTDHAFVPTGHQETSPVICGHCRKLLSLAEYGCGCCPYCQSPFNPACHRHKDIYFLKE
                     S"
     gene            complement(5392..6534)
                     /gene="tgt"
                     /locus_tag="VC122_001435"
     CDS             complement(5392..6534)
                     /gene="tgt"
                     /locus_tag="VC122_001435"
                     /EC_number="2.4.2.29"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011184131.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA guanosine(34) transglycosylase Tgt"
                     /protein_id="HEP5641353.1"
                     /translation="MTDYPIKYRLIKTEKHTGARLGEIITPHGTFPTPMFMPVGTQAT
                     VKTQSPEELKAIGSGIILSNTYHLWLRPGDELIARSGGLHKFMNWDQPILTDSGGFQV
                     YSLADSRNITEEGVTFKNHLNGSKMFLSPEKAISIQNNLGSDIMMSFDECPQFYQPYD
                     YVKKSIERTSRWAERGLKAHRRPHDQGLFGIVQGAGFEDLRRQSAADLVAMDFPGYSI
                     GGLAVGESHEEMNAVLDFTTPLLPENKPRYLMGVGAPDSLIDGVIRGVDMFDCVLPTR
                     IARNGTCMTSEGRLVIKNAKFAEDFTPLDHDCDCYTCQNYSRAYIRHLLKADETFGIR
                     LTSYHNLYFLVNLMKKVRQAIMDDNLLEFRQDFLERYGYNKSNRNF"
     gene            6753..7616
                     /locus_tag="VC122_001436"
     CDS             6753..7616
                     /locus_tag="VC122_001436"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002992548.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF975 family protein"
                     /protein_id="HEP5641354.1"
                     /translation="MSIKAIKGQARDTLKNLSGKYLLFLIPTLLFMFHFGIEIHQGYV
                     LSSGIEVSLAASYFPLLLGVILSLFILSASFTMIDVVRHFRQKVSFAESTTAFSKEFF
                     GNLLVLAITKWLFFLIWSLIWFFGLFIFLSGLSAFLVNAKSGGSTVISLIFLLFGAVL
                     SLIGFGIYINRYYAYSLSEYLLYDKVKEGTYLGAIAVIETSVAMMKGYKWKLFFLQLS
                     FTGWFLLNIVTFGLLNIYLLPYFTTANVIFYDQLKKRFKDKDDPIEGEHLSLHQLNKK
                     PTPMMYDDQKD"
BASE COUNT         2472 a         1607 c         1397 g         2408 t
ORIGIN      
        1 ttataccttt taaagaaact gtctcgactt aaaagcttat catccttatt aaataaatca
       61 acttcataat gtttactatc tttagtagca aggaaaaggc tccccgatgt ataacgcgat
      121 tccgaatcat agatattgta tttttccatt agaaattttc ttattttaaa atcaaattcc
      181 tgtaaagtaa actgtttctt ttttatgcga acagcctcag agggcaaata agtttctttc
      241 ccatcttgat ttaaaattcc tacaaactgt aatgatttat ttccttgatt cgaatgtttt
      301 acaataccac cataaatatt atctacataa ggtggggaaa aattataagg gagaccaaaa
      361 acatcgacct tatccccttt tataaactga tcagtaatgc ctaaagatac aatagaatca
      421 acataaagaa tacattccga tcttttttgt ttagtattaa tatgtatgct atttgtagta
      481 acaaatgcaa tttctacatt ttcataatcg catggggtaa tattataggc aaaccttaaa
      541 cttcttttta aatcttttaa attttcatct gcataagcta attgacttcc atagctaaaa
      601 acacatgata atatgataat tgtcaaaatg tttgttttca tttaaaactt tttatgtcct
      661 gttttatacc tcttaaccta aataaaaata tcaatcggtt tcaacaactg atgacactat
      721 agaaggagga gattacttta gtaaagtcaa caaaaaaaga gcacacaccc gaaaatcgct
      781 tagggctgct ggattcctcc cctgacccac ttcacgcaca agtgttgctc cgcctattat
      841 tatatcatat tacggacagt ttgcaagttt tccaactaag taaaatcact ttcaagtgag
      901 catagcttca ttttgggaca agatggactg aatacctaga aaaatcatga ccctttccaa
      961 acacgatttt gctttttgaa aatctttccc tctccccatt ccagctattc tctatgttat
     1021 aataagatta ttagattaga atgggagtta tgatgaaaca aaagattaaa atcttaacag
     1081 taattggcct tatgacagtt ggtatgtctg cgtgtcacaa cactagcaaa ccttctaata
     1141 ccgattctgt tttttcttta accggaaaaa agcgccaaca aatcgtcaaa caagtaagac
     1201 aacggtatta tttccaacaa ttatccaaaa ccgaacaaga aaattacctc actttatacg
     1261 atagtctagc acaatttcgt gaaatcatca gtttgacgcc ggcttctaaa aaaagcttaa
     1321 ttaaaacgat tgacgccttt gtgatggata atcccgaatt ttactggatt acatctgccg
     1381 attatcgatt tgagttttct gatcaaacgg tctttgtcac ctttcctatc ccagaagatg
     1441 ccaagaacgt ttaccaagac ttgcaagcaa tcggtaatga tatcgtcgca aacacgccat
     1501 caaaggatcg ctatgagcag gtcaaatatt tttatgaggt catcattcga gacacagact
     1561 acaataaaaa agcctttgaa gcctatcaat caggcagtca agcccaggtc gcttctaatc
     1621 aagacattaa aagtgttttt attgatcatt tatctgtttg taatggctat gcccaagcct
     1681 ttcagtttct ttgtcaaaaa gctggcattc cagtcgccta tatccgtgga actggtacat
     1741 ctcagcaacc tcagcaatct tttgcacatg catggaatgc tgttcaaatt aataacactt
     1801 attatggcgt tgatgtcact tggggtgacc ctgtttttga taaccatctc tcacatcaaa
     1861 agcaaggaac tatcaattac agttttctat gcctgccaga ttatctaatg gccttatcac
     1921 atcagccaag caaagacatt gcttttaata ctaaagaacg ttttgaaaat gtttggacta
     1981 taccttcttg tacagatgat tctttgcttt attctaaacg tcatcaaagc tatatctcaa
     2041 cgtttgacag tgacgccatc cttgcaagcc ttgaaaatca gttgttaaat agacaagagc
     2101 aactatgtct acaatttgct catcaagatg attatcaaca gatggtaact gatttaacga
     2161 cgaatcaaac aggttatcat aaccttttca atcagtattg gaataattat acgggcttta
     2221 cctatggact cttaccagag actcttagta tttcatttgc tagtaggaac taggtttaga
     2281 cacatgaaac acaaagttaa gatgattatc tttgtcaaac attacaagaa attgatatac
     2341 tgacacaatt gaaggcgatt attattctaa ccgtcttcaa tagatataac cttttcagat
     2401 tgagcacatc taatgcttgt aacattagca acagtgatga gctctttttt aatgctttag
     2461 caaaatatat cagctaagac aaaagattaa ataactcaag ccatgcctat aagtcacttg
     2521 tttttaatcc ctgtgtcagt gtcaatctac gccacagata actgacagct ctgagcctta
     2581 gaagtctgag ctggataagt ttactaaaaa caaaaataag gtgagccatt actatctgtt
     2641 tttagatagt aaatcttttt aagaatgact aactcatcta gtcatcacgt gttttatttt
     2701 tcgattgttt tcacctcgat tgggtctgaa aaagagcgct tctagtactt ggaaacgccc
     2761 ttttgtatat tctatcttcg tatgttgaat ctaacaacca ttggaaaaat ggtctaacgt
     2821 ttcactcatt attttgacag gttacccttc accaaaaatg catgtgataa caagctcctt
     2881 aaaactctaa tcaaagggat ctgactgttc cttgatcaaa tgcttagcta tttttttgcg
     2941 ctctcgccct tggcggaaaa aggtttgcat gatattagca cagtctgctg ctagcaggcc
     3001 tcgttcaact tgtacgcggt ggtttaagcg ctcatctgtc aaaatctggt aaaggctgtc
     3061 tgctccacca aatttttgat tgctggctcc ataaataaca tgcggaatcc ttgctagccc
     3121 aatggcacca ctacacatca cacaaggctc gatggtcaca aagagtgtgg tgtctaataa
     3181 acgccagttg ccttcatgag cgttagcctc gttaatggcc atcatttcag cgtgcataat
     3241 ggcttggtta ctctcttcac gcgcattgtg cccacgacca ataatctcac catccttgac
     3301 aatgacacag ccaatgggga tttcagcttt ctgtaaggat ttttcggctt cttttaaagc
     3361 ctcttgcatg aaataagttt gctcttctaa actatatggc atcaagaaag actccccaag
     3421 ttccaagatt tgtgtggtaa ggcaaggtca acaggaatat tacctgcata ggcttgtgtt
     3481 tcttgcctca acacttccaa gtgattgtca ggatcaatac cttctggagg aaccggtggc
     3541 atgtgggtca agacaagctt cttaacacct gcctggctag ccattagtcc tgcttcctta
     3601 ctggtcaaat gggccatatg attttcattg ccttcgtata agtaaacatc tgctaagaaa
     3661 aggtctgctt cttttgcgaa gtctgccaat ccttcaaaat aaccactgtc tccagtaaag
     3721 acaagtacct gaccagtcgc tctttctaca attcgaaagg cataacacac aactggatga
     3781 actgttttaa gaaaggtaat gtcaaaagga ccaatctgtt caactccagt gacatcatag
     3841 gctctacctt ctgaaacatc aggaatgctt aacttagcaa attcgtgtcg gtcctcatca
     3901 tgcccataaa tgggcaatac tttaggctgc cacaggtgtt taggataaag ttgaaaataa
     3961 tggcgcaaca cgcctaaatc ggccacgtgg tctggatggt aatggctaat aatcactgca
     4021 tccaaatcga gaggactgat ttctttttcc agctcattga gcgctcgact accagcatcc
     4081 atcaataact gaaagccgtc atgtcctgtg accaaataag aggttgtgcc tgcatcttga
     4141 tagggatagg ctccccaaga ccctaaggtg gttaatttca tctcaaaccc ttctttcttg
     4201 ctttaactct tattataaca aaaattagtg tcaaagagga ttgagaccca ccaaaaacag
     4261 gtcctagcag ataaatctgc caagacctgt tcaacacaac ttaactattt gatgattaga
     4321 ttactatttt gtattcaaaa aactatcttt gtatttaaca gcgataatgg tcgcaatgat
     4381 tgctttgatg gtatctcctg gaataaagac caagtttgac aaaagcgatg tcacaagaga
     4441 catgccagtg taggctgcca accagatagc acccagaaca tcaaccagta caactcctga
     4501 gattaaaaga gctaggaatg ccaatggcgt gctttttgag aaccagttgc ggtaaagtaa
     4561 tgaaaagaca ataggcacaa gaaggtaggc aataacatag ccagcagatg gccccatcaa
     4621 tactggaatg gttgttcgtg agcctgagaa aacaggtaag aaaaggccaa tcactaaaaa
     4681 taagaaaaca gataaggttc cctttttacc acccagcatc aatccagcca acatgacacc
     4741 taagttttgt aaaacaatag ggactgggat aaagcctagc gggatagcag gaataaagcc
     4801 taaaataata atcaaagtgg tcatcatagc caccttaaca agttctttgg ttgtaaacat
     4861 ctgttacgac tcctttaaaa aatagatatc cttgtgccta tgacaggctg gattaaaggg
     4921 actttgacaa taaggacagc atccacaacc atactcagct aggctcaaga gctttcgaca
     4981 atgcccacaa ataacaggag atgtttcttg atgtcctgtt ggcacaaagg catgatctgt
     5041 taactggtca tggcaatggt agcaagcata ataagtctga caaaaagcac atttgagccc
     5101 aacaatatca agcggggtat gataatgtag gcaccgatac tcttggtcaa ggtcaattcc
     5161 aaaacagtcc gtcatatctc atcctcttgt aacagtttaa tcaaaagcat atgatttgtc
     5221 aactctattt ttacaaaagg gtaactgttt tctgaaaaag aacacgtaaa acaagaactt
     5281 ccctttaaag agggacctat cagttgaaag caataggaca tccgtctata caaaaaaact
     5341 gaaaccaaag acgatagatc gccctcagtt ccaggatcat agcattttta attagaaatt
     5401 acggtttgat ttattataac cataccgttc aagaaaatct tggcggaatt caagtaagtt
     5461 atcgtccata atagcctgac gtactttttt catcaagtta accaagaaat aaaggttgtg
     5521 gtagcttgtc aaacgaatac caaaagtttc gtccgctttt agcaagtgac gaatgtaggc
     5581 acggctatag ttttgacaag tgtagcagtc acagtcatga tcaagtgggg taaaatcttc
     5641 agcgaatttg gcatttttaa tcacaagacg cccctcacta gtcatgcaag ttccgttacg
     5701 ggcgatacgc gttggcaaga cacagtcaaa catatctaca ccacgaatca caccatcaat
     5761 caagctatct ggtgctccca ctcccatcag gtaacgaggt ttattttctg gtaagagggg
     5821 agtcgtaaag tcaaggacgg cattcatttc ttcatggctc tccccaacag ctaaaccacc
     5881 aatagaataa cctgggaaat ccatagccac caaatctgcc gcagactgac gacgaaggtc
     5941 ctcaaatcca gctccttgta caattccaaa taatccttgg tcatgaggac gacgatgggc
     6001 ttttaagcca cgttctgccc aacggctagt acgttcaatg gattttttca cgtaatcata
     6061 aggttggtaa aattgcgggc actcgtcaaa actcatcatg atgtctgagc caaggttatt
     6121 ctgaatggaa atggcttttt cgggtgatag aaacattttt gagccattca agtgattttt
     6181 gaaagtcact ccttcttcag tgatgtttcg tgaatcggct aaagagtaaa cttgaaaacc
     6241 acccgaatcg gttaagattg gctgatccca gttcataaac ttgtgcagac caccagagcg
     6301 agcaatcaac tcatctcctg gacgcaacca aaggtgataa gtgtttgata aaataatccc
     6361 tgatccaatt gcttttaact cttctggaga ctgtgtttta acagtagcct gagttcccac
     6421 tggcataaac attggtgtcg gaaaggtccc gtgaggggtg ataatttcgc ccaagcgtgc
     6481 accggtgtgc ttttctgttt tgataaggcg atacttaatc ggataatctg tcatgtgctt
     6541 tcttccttgc tatcaagata aacagtcttg agcttttttg atctgcctga gtttccatca
     6601 taatgaaggg cttcaagatg acatgttgtt ctagcgccta tcattttatc aaaaaatcca
     6661 taatttgtca tgctttccca aaaaagtata gtcaaaataa tggttagctg tctcattttg
     6721 ttttatggta taatgaaata aaggagtctg ttatgtcaat taaagcaatc aaaggccagg
     6781 ctagagacac gttaaaaaat ctctctggca agtatttact atttttaatt ccaacccttc
     6841 tctttatgtt tcatttcggt attgagattc atcaaggtta cgtcctatct agtggtattg
     6901 aagtctcgct agcggctagt tatttcccat tactactagg agttatcctt agccttttca
     6961 ttttatcagc atcctttaca atgattgatg ttgttaggca ttttcgtcag aaagtttctt
     7021 ttgccgaatc aacaacagcc ttctcaaaag aattctttgg gaacctgctg gttttagcga
     7081 tcactaagtg gctattcttt ctcatctggt cactaatttg gttttttggc ctctttattt
     7141 tcctttccgg actttcagcg tttttggtca atgctaaatc aggtggctca acagtcattt
     7201 cactgatctt tttactattt ggagcagttt tgtccttaat tggttttggt atttatatca
     7261 accgttatta cgcttatagt ctatcagaat accttctcta cgataaggtt aaagagggga
     7321 cttatctagg agctatagcc gtcattgaaa caagtgtagc tatgatgaaa ggttataaat
     7381 ggaagctttt tttcttacaa cttagcttca ccggttggtt tctactgaat attgttactt
     7441 ttgggttact taatatctat cttttaccat attttacaac agccaatgtc attttttatg
     7501 atcaactcaa gaaacgtttc aaagacaaag atgacccaat tgaaggagaa catttaagcc
     7561 ttcatcagct taataaaaag cctactccta tgatgtatga cgatcaaaaa gactaaataa
     7621 atcctccatc cataaaaata gcccttgaac tgcaccccaa aagttagaca caaaatctaa
     7681 cgattggggt gctatttttt tatgaaattt aatcaagaaa cgaaagttaa gatttatgag
     7741 ttacggcaaa tgggagagtc cattaaatcc atatcaaaaa agtttgatat ggcagaatcc
     7801 gatctcaaat atatgattcg cttgattgat aggtatgggg taaccattgt tcaaaaatgt
     7861 aagaataatt attattctcc agaa
//
LOCUS       DATXIG010000059         7835 bp    DNA     linear   BCT 28-DEC-2023
DEFINITION  TPA_asm: Streptococcus pyogenes strain MGAS11113
            SAMN08555799-rid19682583.denovo.059, whole genome shotgun sequence.
ACCESSION   DATXIG010000059 DATXIG010000000
VERSION     DATXIG010000059.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN08555799
            Sequence Read Archive: SRR6740495
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pyogenes
  ORGANISM  Streptococcus pyogenes
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 7835)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7835)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-OCT-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-OCT-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001918058.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001918058.2
            Genome Coverage       :: 368x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/12/2023 11:23:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 1,806
            CDSs (total)                      :: 1,769
            Genes (coding)                    :: 1,722
            CDSs (with protein)               :: 1,722
            Genes (RNA)                       :: 37
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1, 1 (5S, 23S)
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 47
            CDSs (without protein)            :: 47
            Pseudo Genes (ambiguous residues) :: 0 of 47
            Pseudo Genes (frameshifted)       :: 30 of 47
            Pseudo Genes (incomplete)         :: 20 of 47
            Pseudo Genes (internal stop)      :: 9 of 47
            Pseudo Genes (multiple problems)  :: 11 of 47
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7835
                     /organism="Streptococcus pyogenes"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN08555799-rid19682583.denovo.059"
                     /strain="MGAS11113"
                     /serotype="Emm28"
                     /isolation_source="normally sterile site"
                     /host="Homo sapiens"
                     /db_xref="taxon:1314"
                     /country="Finland"
                     /collection_date="2002"
                     /collected_by="National Public Health Laboratory"
     gene            10..606
                     /locus_tag="VC122_001437"
     CDS             10..606
                     /locus_tag="VC122_001437"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002993908.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="restriction endonuclease subunit S"
                     /protein_id="HEP5641355.1"
                     /translation="MENSIEAYLADSREKVTLGTVVDCFKGKAVSSKVVPGDVGLVNL
                     SDMGTLGIQYHQLRTFQMDRRQLLRYLLEDGDVLIASKGTLKKVCVFHKQNRDVVASS
                     NITVLRPQKLLRGYYIKFFLDSPIGQALLDAADHGKDVINLSTKELLDIPIPVIPLVK
                     QDYLINHYLRGLTDYHRKLNRAEQEWEYIQNEIQKGLG"
     gene            617..1573
                     /locus_tag="VC122_001438"
     CDS             617..1573
                     /locus_tag="VC122_001438"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014407617.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aromatic acid exporter family protein"
                     /protein_id="HEP5641356.1"
                     /translation="MGSVERTLKMTLATIVAILIAYQLHLDYAMSAGIIALLSVLDTR
                     KSSLVVARNRLLSFFLAFGIAMMCFSLFGFTTVGFMCYLLIIIPLLYHFQIEAGLVPI
                     TVLVTHLIAKKSIALPILSNEFMLFFVGTSVALLFNAYMGPQDQQIRYYHQKVESDLK
                     GILYRFESFLLEGKGQNEGLLIKNLDKILDEALKLVYRERHNQLFQQTNYQVHYFEMR
                     RQQNRLLGQMAINVNTLMRQSKESILLSHLFHETACQLSEQNPALTLIDDIEQLLETF
                     RHGDLPQTREEFERRAVLFQLLQDLERFILLKVEFYQDYQND"
     gene            1670..3010
                     /locus_tag="VC122_001439"
     CDS             1670..3010
                     /locus_tag="VC122_001439"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011528684.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2130 domain-containing protein"
                     /protein_id="HEP5641357.1"
                     /translation="MNEIKCPHCHTLFTINESEYSQLLEQVRGQAFDEELQKRLINEM
                     ALLEEKAKHQLHEVVAKKETAITSLTNQLEQIEKEQAYLRQEELAKKDQLIASLEAKL
                     DKLASQNALELANQLAEKDKEVVSLTNQLDKLALEKDATFQSKLATIEKERDGIKNQL
                     ALQAKESELSLASVRSDYEAQLKAANERVEFYKNFKAQQSTKAIGESLELYAETEFNK
                     VRSYAFPNASFVKDNQLSSRGSKGDYIYREVDANGVEILSIMFEMKNEADTTKTKHKN
                     SDFFKELDKDRREKDCEYAVLVSMLEADNDYYNTGIVDVSHEYQKMYVVRPQLFIQLI
                     GILRNAALNSLHYKQELALIKEQNIDITHFEEDLDQFKNAFAKNYQSASNNFKKAIDE
                     IDKSIKRMEEVRRFLTTSENQLRLANNKLEDVSVKKLTRQNPTMREKFAALKDQ"
     gene            3126..4010
                     /gene="truB"
                     /locus_tag="VC122_001440"
     CDS             3126..4010
                     /gene="truB"
                     /locus_tag="VC122_001440"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002984492.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA pseudouridine(55) synthase TruB"
                     /protein_id="HEP5641358.1"
                     /translation="MINGIINLKKEAGMTSHDAVFKLRKLLQEKKIGHGGTLDPDVVG
                     VLPIAVGKATRVIEYMTEAGKVYEGQVTLGYSTTTEDASGEVVARSSLPAVLTEELVD
                     QTMTTFLGKITQTPPMYSAVKVNGRKLYEYARTGESVERPRREVTISLFERTSPLNFT
                     EDGLCRFSFKVACSKGTYVRTLAVDLGRALWVESHMSFLQRSASAGLTLETAYTLGEI
                     ADMVSKQEMSFLLPIEYGVADLPKMVIDDTELTEISFGRRLSLPSQEPLLAAFHGEKV
                     IAILEKRDQEYKPKKVLI"
     gene            4039..4971
                     /locus_tag="VC122_001441"
     CDS             4039..4971
                     /locus_tag="VC122_001441"
                     /EC_number="2.7.1.26"
                     /EC_number="2.7.7.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002993902.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional riboflavin kinase/FAD synthetase"
                     /protein_id="HEP5641359.1"
                     /translation="MEIEYIKDYRDINQEDDTVLVLGYFDGLHRGHKALFEKAREVAN
                     KEGLKVVVFTFTESPKLAFSRFSPELLLHITYPKKRYEKFADYGVNKLYLVDFTSKFS
                     KVSSDHFITHYIKNLKAKHIVVGFDYKFGHNCTDSDYLTRNFEGQVYTIKEIKEDHRK
                     ISATWIRKLIQEGNVVKANHLLGYDLSTRGRVVHGDARGRTIGFPTANLAPIDNTYLP
                     ADGVYVTNVIVANKIYRSMTSLGKNVTFGGKELRLEVNIFDFDEEIYGEIIEIVWLDK
                     IRDMEKFEGIEDLTDRLEYDKRTALNWKKDSKLS"
     gene            5014..5418
                     /locus_tag="VC122_001442"
     CDS             5014..5418
                     /locus_tag="VC122_001442"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010922362.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Spx/MgsR family RNA polymerase-binding
                     regulatory protein"
                     /protein_id="HEP5641360.1"
                     /translation="MVTLFLSPSCTSCRKARAWLVKHEVDFQEHNIITSPLSRDELMS
                     ILSFTENGTEDIISTRSKVFQKLDVDVEELSISDLIDLIAKNPSLLRRPIIMDQKRMQ
                     IGFNEDEIRAFLSRDYRKQELRQATIKAEIEG"
     gene            5420..5698
                     /locus_tag="VC122_001443"
     CDS             5420..5698
                     /locus_tag="VC122_001443"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002984497.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UPF0223 family protein"
                     /protein_id="HEP5641361.1"
                     /translation="MSGNYYYPLDLSWSTEEISSVLHFLNKVELAYEKKVDAKQLLDS
                     YKTYKTIVKSKAQEKQIDRDFQKVSGYSTYQVVKKAKAIEKGFFSLGN"
     gene            5688..6476
                     /locus_tag="VC122_001444"
     CDS             5688..6476
                     /locus_tag="VC122_001444"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011017816.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="inositol monophosphatase family protein"
                     /protein_id="HEP5641362.1"
                     /translation="METKYAFARQIIKEAGLFIKSKMSEQLDIQVKTQFDDLVTNVDQ
                     ETQQLLMDRIHQTYPCDAILAEENDVRHPINQGNVWVIDPIDGTVNFIVQGSQFAVMI
                     AYYEQGIGQFGLIYDVMADQLLAGGGDFEVTLNGDKLPAYQEKPLERSLIGCNAGMFA
                     RNDRNLAHLIAKTLGVRVYGGAGICMVKVMKQELLAYFSFIQPWDYAAAKVLGDKLGY
                     VLLTIDGYEPDFQTRQKIMFVPKCQLTRIASFLTKDSAVTLEDQ"
     gene            6479..7789
                     /locus_tag="VC122_001445"
     CDS             6479..7789
                     /locus_tag="VC122_001445"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011528680.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RsmF rRNA methyltransferase first C-terminal
                     domain-containing protein"
                     /protein_id="HEP5641363.1"
                     /translation="MMSLPKEFINTYQAILGKELEDFLASFNQEPVNAFRINPLKNQL
                     KTFEHPIPNTLWGYYGKLSGKSPEHVSGLVYSQEPAAQMVAQVAAPQKGSRVLDLAAA
                     PGGKSTHLLAYLDNTGLLVSNEISKKRSKVLVENIERFGARNVVVTNESADRLAKVFS
                     HYFDTIVFDGPCSGEGMFRKDPDAIQYWHHGYPAECAKLQKSILEDALTMLKPGGELI
                     YSTCTWAPEENEDVVQWLLETYTFLELVDVPKLNGMVSGIGLPETARMYPHRYQGEGQ
                     FVAKLKDKRQEGQSTKLKAPKSNLTKDQLRLWKMFEKDHLNLTLSGTLQTFGDYLYLL
                     PDGLPALDGLKIARNGLELGVFKKKRFEPSYALGLALKPDEVRSSIELREEDFRPYVS
                     GNVITLPQSYPTGWYQLLINGNGLGFAKVVDKTIKNNFPKGLRF"
BASE COUNT         2560 a         1319 c         1643 g         2313 t
ORIGIN      
        1 atactatgta tggaaaatag cattgaggcc tatctagcag atagcagaga aaaggtaaca
       61 ttaggtacag ttgttgattg ttttaaagga aaagcagtct caagtaaggt tgtgccaggt
      121 gatgtgggat tggttaattt atctgacatg ggcaccttag ggattcaata tcaccaatta
      181 agaactttcc agatggatcg taggcagctc ttgcgttacc ttttggaaga cggagatgtc
      241 ttaatcgcct ctaagggaac cctaaaaaaa gtctgtgttt ttcataagca aaatcgagat
      301 gtggtggcat catcaaatat cactgtctta cgccctcaaa aactgttaag gggctactat
      361 atcaagttct ttttggactc acctattggt caagcgctct tggacgcagc ggaccatggc
      421 aaagatgtga tcaatctctc aacgaaagaa ttgttggata ttccaattcc cgttattcct
      481 ttagtgaaac aggactattt gattaatcac tacttgagag gattaacaga ttatcatcgt
      541 aaactcaatc gagcagaaca ggagtgggaa tatattcaga atgaaattca aaaaggatta
      601 ggttaggagt cttgtcatgg gttcagttga acgtacatta aaaatgacct tggctaccat
      661 tgttgccatt ttaattgctt accaacttca tctggattat gcaatgtcag cggggatcat
      721 tgccctgtta agtgttttag acacccgtaa gtccagtttg gtggtcgcta ggaatcgctt
      781 gctatctttt ttcctggctt ttgggattgc gatgatgtgc tttagcttgt ttggttttac
      841 gacagtaggt tttatgtgtt atttgttaat tattattcct ttgctctatc actttcagat
      901 agaggcagga ctggttccta taacagtact tgtgacacat ttgattgcta aaaagagtat
      961 tgcattacct attttatcga atgagtttat gctctttttt gtagggacta gtgtcgcttt
     1021 actatttaat gcttatatgg gtccacaaga tcagcaaatt cggtactatc accaaaaagt
     1081 tgaatcggat ctgaaaggta tcttataccg ttttgaaagt tttctactgg aaggaaaagg
     1141 tcaaaatgaa gggctcttga tcaaaaacct tgataaaata ttagatgaag ctctaaaact
     1201 agtttatcga gaaaggcata atcaactatt tcagcagacc aattatcaag ttcattattt
     1261 tgagatgcga cgacaacaaa atagactatt gggacaaatg gctatcaacg taaacacgtt
     1321 aatgagacaa agtaaggaaa gtattctttt gtcacatctt tttcacgaaa ctgcttgtca
     1381 gctaagcgaa caaaatccgg ctttaacctt gattgatgat atcgaacaat tgcttgaaac
     1441 ctttcgtcat ggtgatcttc ctcaaactcg ggaggaattt gagcgacgtg cggttttatt
     1501 tcagctctta caagacttgg aacgttttat cttattaaag gtagagtttt atcaggatta
     1561 tcaaaatgac taaatctcta ttgcacactt cttatctatg acagcttggg acttaacttc
     1621 ctgagctttt ttgctataat aaaaacaaga atctcagaat ggagacaaga tgaacgaaat
     1681 aaaatgccct cattgtcata ccctatttac tatcaatgag tcagagtata gtcaattatt
     1741 ggaacaagtc agagggcaag cctttgatga agaattgcaa aaacggttaa tcaatgagat
     1801 ggccttgctg gaagaaaaag cgaagcatca attgcatgag gttgtcgcaa aaaaagaaac
     1861 agctattacc agcttgacaa accaattaga gcaaattgaa aaagaacaag cctacctcag
     1921 acaagaagaa ctggctaaaa aagaccagct tattgcttct ttagaagcga aacttgataa
     1981 attagcctca caaaatgctt tggaactggc caatcaatta gctgagaagg acaaagaagt
     2041 ggttagtttg accaatcagc tggacaaatt agcacttgaa aaagatgcca cgttccagtc
     2101 aaagcttgct acaatcgaaa aagaacgcga tggaatcaaa aaccaattgg cgcttcaagc
     2161 taaagaaagt gaattgtcgt tggcttcggt gcgcagcgac tacgaagcac agttaaaagc
     2221 ggctaacgag cgagttgaat tttataaaaa tttcaaagcg cagcaatcta ccaaggctat
     2281 cggagagagt ctagaactct atgctgaaac agagtttaac aaagtaagaa gctatgcttt
     2341 tcccaatgct agctttgtca aagataacca attgtctagt cgtgggtcaa aaggggacta
     2401 tatttaccga gaggttgatg ccaatggtgt agaaatcctc tctatcatgt ttgaaatgaa
     2461 gaacgaggca gacaccacca aaacaaaaca taaaaatagt gattttttca aggagttaga
     2521 taaagaccgt cgcgaaaaag attgtgagta tgctgttttg gtaagcatgt tagaagctga
     2581 taatgattac tacaatacag ggattgttga tgtcagtcat gagtatcaaa agatgtatgt
     2641 ggttcgtcct caactcttta ttcagttgat agggattctg cgaaatgcag ccctgaatag
     2701 tttacattat aagcaagagt tggccttgat taaggaacaa aatattgaca tcactcactt
     2761 cgaagaggat ttggatcaat ttaagaatgc ctttgctaag aactatcagt ctgccagcaa
     2821 caactttaaa aaggctattg acgagattga taaatcgatt aaacgcatgg aggaagtgag
     2881 acgcttttta accactagtg aaaatcagtt acgcttagct aataataaat tagaggatgt
     2941 ttcagttaaa aaactgaccc gccaaaaccc aaccatgcga gagaaatttg cagccctcaa
     3001 ggaccagtaa gcctgaaagt gagtctttcc ctgctaaaat tggttaagcg tttattttgg
     3061 caaaactgat gataaagcgg tagaatagaa tcagtaacaa ttactaatta gagagaaaaa
     3121 tagacatgat aaatggaatc attaatttaa aaaaagaagc cggcatgacc tctcatgacg
     3181 cagtttttaa attacggaag ttattacaag aaaaaaagat tggacatggg gggaccttag
     3241 atcctgatgt ggtcggtgtt ctaccaattg cagttggtaa ggctactcgt gtgatcgaat
     3301 acatgacaga agctggtaaa gtttatgaag gacaagtgac tctcggttac tcaacaacga
     3361 ctgaggatgc tagtggagag gtagttgcga gatcttcttt gccagctgtg ctaacagaag
     3421 aactcgttga tcaaacgatg acgacttttc ttgggaaaat cacacagacc ccacctatgt
     3481 attcagccgt aaaggttaat ggccgtaaat tatacgagta tgcgcgtact ggtgaaagtg
     3541 tggaacgtcc tagaagagaa gtgaccattt ctctgtttga acgcaccagc cctttgaatt
     3601 ttactgaaga cggtctttgc cgattctctt ttaaagttgc ttgtagcaag gggacttatg
     3661 tgcggacctt agcagttgat ttaggtcgag ctctatgggt agagagtcat atgtccttct
     3721 tgcagcgatc agcttcagca ggattaaccc tcgagactgc ctatactttg ggagaaattg
     3781 ctgatatggt ttctaaacaa gagatgtctt ttttgttacc gatcgagtat ggcgttgctg
     3841 atcttcctaa aatggttatt gatgatactg aattgactga aatttcattt ggtcgccgtc
     3901 ttagtctccc tagtcaagag ccactgttag ctgcttttca tggcgaaaaa gtaattgcta
     3961 ttttagaaaa aagagatcaa gaatacaaac ccaaaaaagt tcttatttaa atagtttgag
     4021 taagtagaat ggataattat ggaaattgaa tatatcaaag attatcgtga tattaaccaa
     4081 gaagatgata cagtcttggt attgggatat tttgatggat tacaccgtgg tcataaagcc
     4141 ttatttgaaa aagcaagaga ggttgcaaat aaagaagggt taaaagtagt cgtttttacg
     4201 tttacagagt ctccgaaatt ggctttttcg cgtttttctc ctgagttatt gcttcatatt
     4261 acttatccaa agaagcgtta tgaaaaattt gccgattacg gtgtaaataa attatatttg
     4321 gttgatttta cttctaaatt ctctaaggtc tcatctgatc attttataac tcattatatt
     4381 aaaaacctga aagcaaagca tattgttgtc ggttttgact ataagtttgg gcataattgt
     4441 acagatagtg attatttaac cagaaatttt gaaggacaag tttataccat taaggaaata
     4501 aaagaagatc atcgaaaaat ttctgctaca tggataagga agcttattca agaaggtaat
     4561 gtagttaaag caaaccactt gttaggctat gacttatcga caagggggag agtcgttcac
     4621 ggggatgcgc gtggccgtac tattggtttc ccaacagcca atctagctcc aattgacaac
     4681 acatacttac cagctgatgg agtctatgtt acaaatgtta ttgttgctaa taaaatctat
     4741 cgctctatga cgagtttagg taaaaatgtg acatttgggg ggaaagagtt gcgtctagaa
     4801 gttaacattt ttgactttga cgaagagatc tatggagaaa ttattgagat tgtttggtta
     4861 gataagattc gagacatgga aaaattcgag ggaatcgaag atttgactga tagattggaa
     4921 tatgataaac gaacagcatt aaattggaaa aaagatagca aattatctta aatttttgta
     4981 taataaggta agtaaatttg atagaggaaa aatatggtta ccttattttt atcacctagc
     5041 tgtaccagtt gtcgtaaagc aagggcatgg ttagtaaaac acgaagttga ctttcaagag
     5101 cataatatta ttacaagtcc attaagccgt gatgaattaa tgtcaatcct ctcttttaca
     5161 gagaatggaa cggaggatat tatttcaaca cgttcaaagg tttttcaaaa acttgatgtt
     5221 gatgtagaag agctttcaat ttcagattta attgatttaa ttgctaaaaa tccaagtctt
     5281 cttcgtcgcc ctattatcat ggatcaaaaa cgcatgcaaa ttggttttaa tgaagatgaa
     5341 attagagcgt ttttatcgag agattatcgc aagcaagaac tacgtcaagc aacgataaaa
     5401 gcagaaattg aggggtgata tgtcaggtaa ttattattat ccacttgact taagttggag
     5461 cactgaagaa atatcctcag tgcttcattt tttgaataaa gtggagttag catatgaaaa
     5521 aaaagtagat gcaaaacaac tcctagatag ttataagact tataaaacca ttgtcaaaag
     5581 taaagctcag gaaaagcaaa tcgaccgtga ttttcaaaaa gttagtggtt attcgaccta
     5641 tcaagttgtt aaaaaagcga aggcaattga gaaaggattc ttttcccttg gaaactaaat
     5701 acgcatttgc aaggcaaatc attaaagaag cagggctttt tatcaagtca aaaatgtcag
     5761 agcagctaga tattcaggtg aaaacacaat ttgatgatct ggttaccaat gttgatcaag
     5821 aaactcaaca gttactgatg gatcgtattc atcagaccta tccttgtgat gccattttag
     5881 ctgaagaaaa tgacgttcgt caccccatta atcaaggaaa tgtttgggtc atcgatccca
     5941 ttgatggaac ggttaacttt attgtgcagg gatcacagtt tgcagtgatg attgcctatt
     6001 atgaacaagg aattggccaa tttgggctta tttatgatgt gatggcagat caattactag
     6061 ctggtggtgg tgattttgaa gttactttaa acggtgataa gcttccagct taccaagaaa
     6121 aacctttaga gcgtagctta attggctgca atgcaggcat gtttgccaga aatgatcgta
     6181 accttgctca tctaattgca aagactttag gtgtaagggt ttatggtggt gcaggtatct
     6241 gcatggttaa ggtcatgaag caggaattac tggcctattt ttcatttatc caaccatggg
     6301 attacgcagc agcaaaagta ctaggcgata aactgggtta tgttttacta actattgacg
     6361 gatatgagcc tgattttcaa acacgtcaaa aaatcatgtt tgttcctaaa tgccaactca
     6421 ctaggatagc gtcttttcta acaaaggact ctgccgtcac acttgaagat caatagaaat
     6481 gatgtcatta ccaaaagaat ttattaacac ttatcaagca atactaggca aagaattaga
     6541 agatttcttg gcaagtttta accaagagcc ggtcaatgct tttcggatta accccttaaa
     6601 gaatcaacta aaaacctttg aacaccccat tccaaatacc ttatggggat attatggtaa
     6661 actatctggc aagtctccag agcatgtgtc ggggttggtt tattcccaag aaccagcggc
     6721 tcaaatggtg gcacaagtag cggctcctca aaaggggagt cgtgttttag acttggcagc
     6781 tgcaccagga ggaaaatcaa ctcatttatt agcttattta gacaatacag gtcttttagt
     6841 cagcaatgaa atttctaaga agcgcagcaa ggttttagtt gaaaatatcg aacgttttgg
     6901 cgctagaaat gttgtggtga ctaatgaatc cgcagatcgt ttagccaagg tattttctca
     6961 ttattttgat actatcgtct ttgatggacc atgttctggt gaaggaatgt ttcgcaagga
     7021 ccctgatgct attcaatatt ggcatcacgg gtatcccgca gaatgcgcca agttgcaaaa
     7081 atctatttta gaagacgcct taaccatgtt aaaaccagga ggagaattga tatactccac
     7141 ctgtacttgg gctcctgaag aaaatgaaga tgttgtgcag tggctcttag agacttatac
     7201 ctttttagaa ttggtcgatg ttccaaaact aaatggaatg gttagtggta tcggacttcc
     7261 agagactgct agaatgtacc ctcatcgtta tcaaggggaa gggcaatttg tggccaagct
     7321 aaaagataag cgtcaagaag gacaaagcac taaactaaaa gcacctaaat ccaacttgac
     7381 caaagaccag ttacgtcttt ggaaaatgtt tgaaaaagac catctaaacc ttaccctatc
     7441 aggaacacta caaacctttg gtgattacct atacctcttg ccagatggtc ttcctgcttt
     7501 agatggcctt aaaattgctc ggaacggttt agaattaggc gtcttcaaga agaaacgctt
     7561 tgaaccctct tacgccttgg gattagcttt aaagcctgat gaggtccgat catctattga
     7621 gcttagagag gaagacttta gaccttatgt gtcaggtaac gtcattactt tgccccaatc
     7681 ttatccaaca ggctggtacc aattactcat taatggcaat ggtctaggat ttgccaaggt
     7741 cgttgacaag accatcaaaa ataatttccc taaagggtta agattttagc gtaaaaaata
     7801 atattaaaaa gttaaatatt taaagaaaaa atgct
//
LOCUS       DATXIG010000060         7656 bp    DNA     linear   BCT 28-DEC-2023
DEFINITION  TPA_asm: Streptococcus pyogenes strain MGAS11113
            SAMN08555799-rid19682583.denovo.060, whole genome shotgun sequence.
ACCESSION   DATXIG010000060 DATXIG010000000
VERSION     DATXIG010000060.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN08555799
            Sequence Read Archive: SRR6740495
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pyogenes
  ORGANISM  Streptococcus pyogenes
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 7656)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7656)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-OCT-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-OCT-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001918058.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001918058.2
            Genome Coverage       :: 368x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/12/2023 11:23:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 1,806
            CDSs (total)                      :: 1,769
            Genes (coding)                    :: 1,722
            CDSs (with protein)               :: 1,722
            Genes (RNA)                       :: 37
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1, 1 (5S, 23S)
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 47
            CDSs (without protein)            :: 47
            Pseudo Genes (ambiguous residues) :: 0 of 47
            Pseudo Genes (frameshifted)       :: 30 of 47
            Pseudo Genes (incomplete)         :: 20 of 47
            Pseudo Genes (internal stop)      :: 9 of 47
            Pseudo Genes (multiple problems)  :: 11 of 47
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7656
                     /organism="Streptococcus pyogenes"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN08555799-rid19682583.denovo.060"
                     /strain="MGAS11113"
                     /serotype="Emm28"
                     /isolation_source="normally sterile site"
                     /host="Homo sapiens"
                     /db_xref="taxon:1314"
                     /country="Finland"
                     /collection_date="2002"
                     /collected_by="National Public Health Laboratory"
     gene            91..798
                     /locus_tag="VC122_001446"
     CDS             91..798
                     /locus_tag="VC122_001446"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002984000.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="O-methyltransferase"
                     /protein_id="HEP5641364.1"
                     /translation="MVKSYSKTANHNMRRPVVKEELVHYMRTRQKQITGFLAELEQFA
                     RQENIPIIQPEVVAYFRFLLQSLQPKHILEIGTAIGFSALLMAENAPDATIVTIDRNR
                     EMIDFAKANFAKYDSRQQIRLLEGDAADILSTLEGNFDFVFMDSAKSKYIVFLPEILR
                     LLKVGGVVILDDVFQGGDITKPIEDIRRGQRTIYRGLQSLFDATLTHPNLTTSLVPLS
                     DGLLMIRKNQADILLPD"
     gene            861..1916
                     /gene="prsA"
                     /locus_tag="VC122_001447"
     CDS             861..1916
                     /gene="prsA"
                     /locus_tag="VC122_001447"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011184661.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase PrsA"
                     /protein_id="HEP5641365.1"
                     /translation="MKNSNKLIASVVTLASVMALAACQSTNDNTKVISMKGDTISVSD
                     FYNETKNTEVSQKAMLNLVISRVFEAQYGDKVSKKEVEKAYHKTAEQYGASFSAALAQ
                     SSLTPETFKRQIRSSKLVEYAVKEAAKKELTTQEYKKAYESYTPTMAVEMITLDNEET
                     AKSVLEELKAEGADFTAIAKEKTTTPEKKVTYKFDSGATNVPTDVVKAASSLNEGGIS
                     DVISVLDPTSYQKKFYIVKVTKKAEKKSDWQEYKKRLKAIIIAEKSKDMNFQNKVIAN
                     ALDKANVKIKDKAFANILAQYANLGQKTKAASESSTTSESSKAAEENPSESEQTQTSS
                     AEEPTETEAQTQEPAAQ"
     gene            2303..4921
                     /gene="alaS"
                     /locus_tag="VC122_001448"
     CDS             2303..4921
                     /gene="alaS"
                     /locus_tag="VC122_001448"
                     /EC_number="6.1.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000661542.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alanine--tRNA ligase"
                     /protein_id="HEP5641366.1"
                     /translation="MKELSSAQIRQMWLDFWKSKGHCVEPSANLVPVNDPTLLWINSG
                     VATLKKYFDGSVIPENPRITNAQKSIRTNDIENVGKTARHHTMFEMLGNFSIGDYFRD
                     EAIEWGFELLTSPDWFDFPKDKLYMTYYPDDKDSYNRWIACGVEPSHLVPIEDNFWEI
                     GAGPSGPDTEIFFDRGEDFDPENIGLRLLAEDIENDRYIEIWNIVLSQFNADPAVPRS
                     EYKELPNKNIDTGAGLERLAAVMQGAKTNFETDLFMPIIREVEKLSGKTYNPDGDNMS
                     FKVIADHIRALSFAIGDGALPGNEGRGYVLRRLLRRAVMHGRRLGINETFLYKLVPTV
                     GQIMESYYPEVLEKRDFIEKIVKREEETFARTIDAGSGHLDSLLAQLKAEGKDTLEGK
                     DIFKLYDTYGFPVELTEELAEDAGYKIDHEGFKSAMKEQQDRARAAVVKGGSMGMQNE
                     TLAGIVEESRFEYDTYSLESSLSVIIADNERTEAVSEGQALLVFAQTPFYAEMGGQVA
                     DTGRIKNDKGDTVAEVVDVQKAPNGQPLHTVNVLASLSVGTNYTLEINKERRLAVEKN
                     HTATHLLHAALHNVIGEHATQAGSLNEEEFLRFDFTHFEAVSNEELRHIEQEVNEQIW
                     NALTITTTETDVETAKEMGAMALFGEKYGKVVRVVQIGNYSVELCGGTHLNNSSEIGL
                     FKIVKEEGIGSGTRRIIAVTGRQAFEAYRNQEDALKEIAATVKAPQLKDAAAKVQALS
                     DSLRDLQKENAELKEKAAAAAAGDVFKDVQEAKGVRFIASQVDVADAGALRTFADNWK
                     QKDYSDVLVLVAAIGEKVNVLVASKTKDVHAGNMIKELAPIVAGRGGGKPDMAMAGGS
                     DASKIAELLAAVAETV"
     gene            5282..5497
                     /locus_tag="VC122_001449"
     CDS             5282..5497
                     /locus_tag="VC122_001449"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003051509.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HEP5641367.1"
                     /translation="MEVILKNRLKELRARDGINQTEMAKLAGVSRQTISLIERNEYTP
                     SVIIAMKIAKVFQEPVEEVFRLVEVEE"
     gene            5494..6255
                     /locus_tag="VC122_001450"
     CDS             5494..6255
                     /locus_tag="VC122_001450"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010922423.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3169 family protein"
                     /protein_id="HEP5641368.1"
                     /translation="MKKKKNSFLRLLKMLLLSSLAGGIIGGMVGAFLGYHGERLDQLT
                     FLKDDVINLIILLNRLVVVTGLTLSFVFLTQLKKETAVYNTIEEDDYSENGYRQLNKK
                     HAYTMLLIAVASILSMCNVLLGLTLTNDSQHAMLAIPLLDILLLLMVIPFQALAMKRY
                     NAIRGTDVPYFPNLKELKHNIMALDEAELQAYHKTSFESVLSLNGVIIPSLYVILFFV
                     YLFTGQVELTAILVLVLIQLYLLVKSATMTRQFYR"
     gene            6274..6969
                     /locus_tag="VC122_001451"
     CDS             6274..6969
                     /locus_tag="VC122_001451"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011184658.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CPBP family intramembrane metalloprotease"
                     /protein_id="HEP5641369.1"
                     /translation="MKGFINYLKIAVLIILAMVFNVLPMILLQKQHDIPMVLNWGIGI
                     FYLVIVGSVLIVLWRLYQAKQDTPIKQQKMRLVDWGYLALFWLIIRVIAIAGTLVNQL
                     WSGQQVSANDAAIHTLARLIKGGFPLYTALFVLVIAFIAPIMEELVFRGFPMIDLFKG
                     KSLKVAGLVTSLVFALPHATNSVEFIMYSCMGIFLFVAYQRRGNLKDAILLHIFNNLI
                     EVILLMSIGLGVI"
     gene            complement(7063..7590)
                     /locus_tag="VC122_001452"
                     /pseudo
     CDS             complement(7063..7590)
                     /locus_tag="VC122_001452"
                     /inference="COORDINATES: protein motif:HMM:NF033516.0"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS3 family transposase"
BASE COUNT         2431 a         1338 c         1588 g         2299 t
ORIGIN      
        1 ggtagcatta aggttaaaga aatgatgtcc tataaaaaat ttaaaagtaa accctagcaa
       61 agcctagggt ttttttatat aattaggttt atggttaaat catacagtaa aacagcaaat
      121 cataatatgc gtcgtcctgt tgtcaaagaa gagcttgttc attatatgcg aacacggcag
      181 aagcaaatca caggtttttt agcagagtta gagcaattcg caagacaaga aaacattcct
      241 attattcagc ctgaggtcgt ggcttatttc aggtttttat tgcaaagctt acagccaaaa
      301 cacattttgg agattggcac agccattggt ttttctgcgc ttttaatggc tgaaaatgca
      361 ccagatgcga cgattgtgac tattgatcgt aatcgagaga tgatcgattt tgctaaagca
      421 aattttgcta agtacgacag tcgccaacag atccgcctgt tagaaggaga tgcagcagat
      481 atattatcta cacttgaagg aaattttgac tttgtgttta tggattccgc caaatccaaa
      541 tatattgtct ttctaccaga gattttaaga ctgttaaaag tcggtggtgt agtcattttg
      601 gatgatgtct ttcaaggagg tgacattaca aaaccgatcg aagacattcg acgaggtcag
      661 cgtactattt atcgtggctt acagtctttg tttgatgcta ccttaactca tccaaaccta
      721 acaactagcc tggtccctct tagtgatgga ttattaatga ttcgtaaaaa tcaagcagat
      781 attctattac cagattaagt aacattgata aatttatgtt ataataaatt agttaaagat
      841 aaaaaggagc tcagaagtaa atgaaaaact caaataaact cattgctagt gttgtgacat
      901 tggcctcagt gatggcttta gcagcttgtc aatcaactaa tgacaatact aaggttattt
      961 cgatgaaagg tgatacaatt agcgttagtg atttttacaa tgaaacaaag aacacagaag
     1021 tatcgcaaaa agcgatgcta aatctggtaa ttagtcgtgt ttttgaagct caatatggtg
     1081 ataaggtttc aaaaaaagaa gttgaaaagg cgtatcataa aacagctgaa cagtatggcg
     1141 cttcattctc tgctgctttg gcacaatcaa gcttgacacc tgagactttt aagcgtcaga
     1201 tccgctcttc aaaattagta gaatatgcgg ttaaagaagc agctaaaaaa gaattgacaa
     1261 cacaagaata taagaaagca tatgaatctt atactccaac aatggcagtc gaaatgatta
     1321 ctttagataa tgaagagaca gctaaatcag tcttagagga actaaaagcc gaaggcgcag
     1381 actttacagc tattgctaaa gaaaaaacaa caacacctga gaaaaaagtg acctataaat
     1441 ttgattcagg cgcgacaaat gtaccgactg atgtcgtaaa agcggcttca agtttgaatg
     1501 agggtggcat atcagacgtt atctcggttt tagatccaac ttcttatcaa aagaagtttt
     1561 acattgttaa ggtgactaaa aaagcagaaa aaaaatcaga ttggcaagaa tataagaaac
     1621 gtttgaaagc tatcattata gctgaaaaat caaaagatat gaatttccaa aacaaggtta
     1681 ttgcaaatgc attggataaa gctaatgtaa aaattaaaga caaagctttt gctaatattt
     1741 tggcgcaata tgcaaatctt ggtcaaaaaa ctaaagctgc aagtgaaagt tcaacaacca
     1801 gcgaatcatc aaaagctgca gaagagaacc catcagaatc agagcaaaca cagacatcat
     1861 cagctgaaga accaactgag actgaggctc agacgcaaga gccagctgca caataagtag
     1921 gattttattg actaatcagt taaaaaaact tataataatt tagttaagaa ttgtttatca
     1981 aggattagat acagaaaagt gacggttgct gcgagtcatt gataattaga taaacatgct
     2041 acttaacttt acaacaattg atatcgtaaa ttaagaatga caagttcatt gaatagaggt
     2101 ggtaccgcgg ttttcgccct ctgtgagatg gacttgtttt gtattgagga ctatttgaaa
     2161 tcaacgaaag gaatagagta gactaggctg ccaggcttat agctgatctc ctagcaagaa
     2221 agacgagctg tgtcaactgg tgtgattaca ttactagctt cagcataaca ctgtttttct
     2281 tactctttgt atctaaattt ttatgaaaga attatcgtct gcacaaatcc gccaaatgtg
     2341 gttggatttc tggaaatcta aaggacattg cgttgagcct tcagctaact tggttcctgt
     2401 gaatgaccca acgcttcttt ggatcaactc aggtgttgca accttgaaaa aatactttga
     2461 tggttcagtg attccagaaa atccacgtat taccaatgca caaaaatcaa ttcgtactaa
     2521 tgatattgaa aatgttggta aaacagcacg tcaccatact atgtttgaaa tgcttggtaa
     2581 cttctcaatt ggagactatt tccgtgatga agctattgag tggggatttg aactcttgac
     2641 aagtccagac tggtttgatt tccctaaaga taagctctac atgacttatt acccagatga
     2701 caaggattca tataaccgtt ggattgcttg tggcgttgaa ccaagtcact tggtgccgat
     2761 cgaggataac ttctgggaaa tcggtgctgg tccttcaggt ccagatacgg agattttctt
     2821 cgatcgtggt gaagatttcg atccagaaaa tatcggactt cgcctcttgg ctgaagatat
     2881 cgaaaacgat cgttacatcg aaatctggaa catcgttctc tcacaattca atgctgaccc
     2941 agccgtacca cgttcagaat acaaagaatt accaaacaaa aacattgata caggtgctgg
     3001 tcttgaacgt cttgcagctg ttatgcaagg ggcaaaaaca aactttgaaa ctgacctctt
     3061 catgccaatc atccgtgaag tagagaagtt gtcaggtaaa acttataatc cagatggcga
     3121 caacatgagt ttcaaggtta tcgctgacca catccgtgcg ctttcatttg ctatcggtga
     3181 tggtgcgctt cctggaaatg aaggtcgtgg ttacgttctt cgtcgtcttc tccgtcgtgc
     3241 tgttatgcat ggtcgtcgtc ttggcatcaa cgaaactttc ctttacaaat tggttccgac
     3301 tgttggacaa atcatggaaa gctactaccc agaagtgctt gaaaaacgtg attttatcga
     3361 gaaaatcgtt aaacgtgagg aagaaacatt tgctcgtact atcgatgcag gtagcggtca
     3421 cttagattca ttgcttgcgc agcttaaggc tgaaggtaag gatactcttg aaggtaaaga
     3481 tatcttcaaa ctttatgata cttatggatt cccggttgaa ttgacagagg aattggcaga
     3541 agatgcaggc tacaagattg atcacgaagg ctttaagtca gccatgaaag aacaacaaga
     3601 ccgtgcgcgt gcagctgttg ttaagggtgg ttcaatgggg atgcaaaatg aaaccctagc
     3661 tggtattgtt gaagaatcac gattcgaata cgacacatat agtcttgaat caagtctttc
     3721 agtcatcatc gctgataatg aacgtaccga agctgtttca gaaggtcaag ctcttcttgt
     3781 ctttgctcaa acaccattct atgctgaaat gggtggacag gttgctgaca caggtagaat
     3841 caaaaatgat aagggtgaca cagttgctga ggttgttgat gttcaaaaag caccaaatgg
     3901 tcaacctcta cacactgtaa acgttttggc atcactttca gttggaacaa actacacact
     3961 tgaaatcaac aaagagcgtc gtttggctgt tgagaaaaac cacacagcaa ctcacttgct
     4021 tcatgcagcc cttcacaatg ttatcggtga acacgcaact caggctggtt cattgaacga
     4081 agaagaattc ttgcgctttg attttactca ctttgaagca gtaagcaatg aggaacttcg
     4141 tcacattgaa caagaagtta atgagcaaat ttggaacgct cttacgatca caacgactga
     4201 aactgacgtt gaaaccgcaa aagagatggg agcaatggca ctttttggtg agaaatatgg
     4261 taaagtggtt cgtgtggttc aaattggtaa ttattctgtt gaactttgtg gtggaactca
     4321 cttaaataat tcttcagaaa tcggtctctt caagattgtc aaagaagaag gtattggttc
     4381 aggcactcgt cgtattattg cagttactgg tagacaagct tttgaagctt atcgtaacca
     4441 agaggatgcc ctaaaagaga tcgctgctac tgtaaaagct ccgcaattga aagatgcagc
     4501 agctaaagta caagctctta gcgactcact tcgtgatctt caaaaagaaa atgcagagct
     4561 taaagaaaaa gcagcagctg cagcagctgg tgatgtcttt aaagatgttc aagaagctaa
     4621 gggcgtgcgc ttcattgcta gtcaagttga tgttgcagat gcaggggcac ttcgtacatt
     4681 tgctgataac tggaaacaaa aagactactc tgatgtgctt gttctcgtag cagctattgg
     4741 tgagaaggtt aatgtccttg ttgcaagcaa aactaaagat gtccacgctg gtaacatgat
     4801 caaagaattg gcaccaattg tagcaggtcg tggtggaggt aaaccagaca tggctatggc
     4861 aggtggtagc gatgcaagta aaattgcaga gctgctagca gcagttgctg aaacagtgta
     4921 aatccataaa aaaccaagtt atttagaact tggtttttta tatttgaaat gactaccatc
     4981 ataacctttt tgactattat cgttgtcaaa aagaaaaaga tatgttacaa ttatagtaac
     5041 aaatagacat gaggaggtgt tagagaaaat gacacatggt atcaaactta agaggcagat
     5101 ttgcttatgg gcaagtatcc tgactctttt cattttaagt tatcatctta tcagggtaat
     5161 ctggtttgga gtacccttga gttatctggt tttgggcttt acaggactag ccttgctgtt
     5221 gaatactttt tcttatttta gaaagtaagt tataagttaa agaagaatga aggagatggc
     5281 aatggaggtt attcttaaaa atcggctcaa agaattacga gccagagatg gaattaatca
     5341 aacggaaatg gctaaactgg caggagtatc gaggcagacc atcagtctca ttgagcgtaa
     5401 cgagtacacc ccatcagtta taattgccat gaaaattgca aaggtatttc aggaaccagt
     5461 tgaagaggtg tttcgtctag tggaggtcga ggaatgaaaa agaagaagaa tagtttttta
     5521 cgcttgttaa aaatgttatt gctatcaagt ttggcgggag ggattatagg tggcatggtt
     5581 ggagcctttt taggttacca tggagagaga ttagatcagc tcacgttcct aaaagatgat
     5641 gtgattaacc tgattatctt attaaatcgt ctagtagttg tcacaggcct cacactttcc
     5701 tttgtttttt taacgcaact gaaaaaagag acagctgttt ataacacaat tgaagaggat
     5761 gattattctg aaaatggtta ccgtcagctg aataaaaagc atgcttatac catgttattg
     5821 attgccgtag cttctatttt atccatgtgc aatgtgctac ttggtctgac cttaactaat
     5881 gattcacagc atgccatgct ggccattccg cttctggata tccttttatt attaatggtc
     5941 attccttttc aagctttagc catgaaacgt tacaatgcca ttcggggaac cgatgttcct
     6001 tattttccaa atttgaaaga attaaaacat aatatcatgg ctctagatga ggcagagtta
     6061 caagcctatc ataagacatc gtttgaaagt gtgttgtcat taaatggagt catcatccct
     6121 tccttgtatg tgattttatt ttttgtctat ctattcactg gtcaagtgga gttaacagcc
     6181 atcttagttt tggtgttaat tcagctctat ttactcgtta agagtgcaac aatgaccaga
     6241 caattttacc gttagcctaa aaggagactg tttatgaaag gattcattaa ttatttaaaa
     6301 atagcagtgc tcattattct ggctatggtt ttcaatgtcc ttccgatgat tttattgcaa
     6361 aagcaacacg atattcctat ggtgcttaat tggggaattg gtattttcta cttggttatt
     6421 gttggaagtg tccttattgt attatggcgt ctttatcaag ctaagcaaga cacccctatt
     6481 aaacagcaaa aaatgagatt ggttgactgg ggttatttag cattattttg gttaatcatc
     6541 cgtgtgatag ctattgcagg tacccttgtt aaccagctat ggtctggtca acaagtgagt
     6601 gctaatgatg ctgcaataca taccttagct agacttatca aaggtggttt cccgctttat
     6661 actgccctat ttgtacttgt gatagctttt atcgctccta ttatggaaga actagtcttt
     6721 agaggatttc ctatgattga tctcttcaaa ggaaaatcac ttaaggtggc aggtttagtg
     6781 acctctcttg tttttgcttt accacatgcc accaatagtg ttgaatttat catgtacagc
     6841 tgtatgggca tttttctctt tgttgcctat caaagacgag gaaacttaaa agacgctatc
     6901 ttgttacata tttttaataa cttgattgaa gtcattttgt taatgtcaat aggcttagga
     6961 gtcatataat aaaaagcttc ggatggatga actgcacccc aaaagttgga caaaaaaatc
     7021 taacatttgg gttggaattg tcaagaaaaa atagacataa agttaagcta atgagtaact
     7081 atttttttca aattcaacag gtgagagata ccctaaagct gaatgcagac gtttggggtt
     7141 atagtaagtc tcactatatt taaaaatctc aagcttagct tgctcaatag ttgcgaaatg
     7201 agcatcattg acaagctctc tttttaatgt tttataaaag gattccatga gtgcattatc
     7261 gtatggatta ccttttcgac tcatcctgga cttacatttt ttccatctaa gtaggtcctg
     7321 aaatcgtgct ccggtatatt gagaaccctg atctgtatgg acgattaccc cttctttagg
     7381 cttttctcta ttataagctt gattgaaagc ttctgttacc aacttgtctt gcatacgttg
     7441 gcccattgcc caaccaacaa cctttctact ataaacgtct atcaagacag acacataaag
     7501 tattccttct tgtgtaggaa tgtaggttag gtctcctagc cataatttat tcttaccagt
     7561 cgcttggaaa cattgattaa caagattggg acgagttcat gaagaacgtc gacggttata
     7621 gtatttatat tgatatcgac ttcctttagc atagag
//
LOCUS       DATXIG010000061         7573 bp    DNA     linear   BCT 28-DEC-2023
DEFINITION  TPA_asm: Streptococcus pyogenes strain MGAS11113
            SAMN08555799-rid19682583.denovo.061, whole genome shotgun sequence.
ACCESSION   DATXIG010000061 DATXIG010000000
VERSION     DATXIG010000061.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN08555799
            Sequence Read Archive: SRR6740495
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pyogenes
  ORGANISM  Streptococcus pyogenes
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 7573)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7573)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-OCT-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-OCT-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001918058.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001918058.2
            Genome Coverage       :: 368x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/12/2023 11:23:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 1,806
            CDSs (total)                      :: 1,769
            Genes (coding)                    :: 1,722
            CDSs (with protein)               :: 1,722
            Genes (RNA)                       :: 37
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1, 1 (5S, 23S)
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 47
            CDSs (without protein)            :: 47
            Pseudo Genes (ambiguous residues) :: 0 of 47
            Pseudo Genes (frameshifted)       :: 30 of 47
            Pseudo Genes (incomplete)         :: 20 of 47
            Pseudo Genes (internal stop)      :: 9 of 47
            Pseudo Genes (multiple problems)  :: 11 of 47
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7573
                     /organism="Streptococcus pyogenes"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN08555799-rid19682583.denovo.061"
                     /strain="MGAS11113"
                     /serotype="Emm28"
                     /isolation_source="normally sterile site"
                     /host="Homo sapiens"
                     /db_xref="taxon:1314"
                     /country="Finland"
                     /collection_date="2002"
                     /collected_by="National Public Health Laboratory"
     gene            <1..385
                     /locus_tag="VC122_001453"
     CDS             <1..385
                     /locus_tag="VC122_001453"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002985164.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="DUF2142 domain-containing protein"
                     /protein_id="HEP5641370.1"
                     /translation="QFGALSYGITNLFPLYVCFFFFVYISNASKITINIVEKMGIIFV
                     ISAIIGATVLAMYLTWTPVGSSTVLGVQSRYLIGIIPLVLLLFSSQQQKFKQIEDILS
                     DKLAIHVSLLFILAMLMSTIFRYYH"
     gene            479..1702
                     /gene="pepT"
                     /locus_tag="VC122_001454"
     CDS             479..1702
                     /gene="pepT"
                     /locus_tag="VC122_001454"
                     /EC_number="3.4.11.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011054346.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidase T"
                     /protein_id="HEP5641371.1"
                     /translation="MKYDNLLDRFIKYVKVNTRSDPDSETTPSTESQEAFALTILKPE
                     MEAIGLQDVHYNPVNGYLIGTLPANNPTLTRKIGFIAHMDTADFNAENVNPQIIDNYQ
                     GGDITLGSSNYKLDPKAFPNLNNYIGQTLITTDGTTLLGADDKSGIAEIMTAIEFLTS
                     QPQIEHCDIKVAFGPDEEIGVGADKFEVADFEVDFAYTMDGGPLGELQYETFSAAALE
                     VTFLGRNVHPGTAKDQMINALELAIDFHEKLPAKERPEYTDGYQGFYHLTGLTGTVEE
                     ARASYIIRDFEEASFEARKVKVENIAQSMNAQLGTKRVLVELNDQYYNMKKVIEKDMT
                     AIELAKEVMEELAIKPVIEPIRGGTDGSKISFMGIPTPNIFAGGENMHGRFEFVSLQT
                     MERAVDVIIGLVCKA"
     gene            1743..2231
                     /locus_tag="VC122_001455"
     CDS             1743..2231
                     /locus_tag="VC122_001455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011889008.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EbsA family protein"
                     /protein_id="HEP5641372.1"
                     /translation="MIKLFGKIRYHWQPELSWSIIYWSIAFAPIFVGLSLLYERTEIP
                     SRVFILFAIFAVLVGIGLHRYFIIENNGILRIVSFKLFGPRKLLISTITKIEVTKSTL
                     CLHFEDKSYLFYMRKWPKKYFLDALAVNPYFQGEVILSDNFIKLDYFEVYQHDKKALT
                     RG"
     gene            complement(2218..2415)
                     /locus_tag="VC122_001456"
     CDS             complement(2218..2415)
                     /locus_tag="VC122_001456"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014407483.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferredoxin"
                     /protein_id="HEP5641373.1"
                     /translation="MKVSIIPEKCIACGLCQTYSSLFDYHDNGIVTFSSSSETSQSIC
                     PSDKDAILAVKSCPTKALTLE"
     gene            2464..2940
                     /locus_tag="VC122_001457"
     CDS             2464..2940
                     /locus_tag="VC122_001457"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002990270.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysM peptidoglycan-binding domain-containing
                     protein"
                     /protein_id="HEP5641374.1"
                     /translation="MAKEPWEEKIVDDTIGTRTRKSRNAFISTPWLTALLSVFFVIIV
                     AILFIFFYTSNSGSNRQAETNGFYGASTHKKTRKASNAKKASSSSTTTDTTPSSEETL
                     ASSEGTGETLTVLAGEGAASIAARAGISVEQLQALNPEHMTQGYWYANPGDQVTIK"
     gene            2955..3635
                     /locus_tag="VC122_001458"
     CDS             2955..3635
                     /locus_tag="VC122_001458"
                     /EC_number="2.7.4.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003050177.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="(d)CMP kinase"
                     /protein_id="HEP5641375.1"
                     /translation="MKAIKIAIDGPASSGKSTVAKIIAKNLGYTYLDTGAMYRSATYI
                     ALTHGYTGKEVALILEELEKNPISFKKAKDGSQLVFLGDEDVTLAIRQNDVTNNVSWV
                     SALPEIREELVHQQRRIAQAGGIIMDGRDIGTVVLPDAELKIFLVASVEERAERRYKE
                     NLEKGIESDFETLKEEIAARDYKDSHRKVSPLKAAEDALIFDTTGVSIDGVVQFIQEK
                     AEKIVDMS"
     misc_feature    3659..3782
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00558"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="ribosomal protein L20 leader region; Derived by
                     automated computational analysis using gene prediction
                     method: cmsearch."
                     /db_xref="RFAM:RF00558"
     gene            3797..4327
                     /gene="infC"
                     /locus_tag="VC122_001459"
     CDS             3797..4327
                     /gene="infC"
                     /locus_tag="VC122_001459"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012515489.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="translation initiation factor IF-3"
                     /protein_id="HEP5641376.1"
                     /translation="MKIIAKKDLFINDEIRVREVRLVGLEGEQLGIKPLSEAQSLADA
                     SNVDLVLIQPQAVPPVAKLMDYGKFKFEYQKKQKEQRKKQSVVTVKEVRLSPVIDKGD
                     FETKLRNGRKFLEKGNKVKVSIRFKGRMITHKEIGAKVLADFAEATQDIAIIEQRAKM
                     DGRQMFMQLAPISDKK"
     gene            4369..4566
                     /gene="rpmI"
                     /locus_tag="VC122_001460"
     CDS             4369..4566
                     /gene="rpmI"
                     /locus_tag="VC122_001460"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002895038.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L35"
                     /protein_id="HEP5641377.1"
                     /translation="MPKQKTHRASAKRFKRTGSGGLKRFRAFTSHRFHGKTKKQRRHL
                     RKAGLVSSGDFKRIKAMVTGL"
     gene            4625..4984
                     /gene="rplT"
                     /locus_tag="VC122_001461"
     CDS             4625..4984
                     /gene="rplT"
                     /locus_tag="VC122_001461"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019786714.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L20"
                     /protein_id="HEP5641378.1"
                     /translation="MARVKGGVVSRKRRKRILKLAKGYYGAKHILFRTAKEQVMNSYY
                     YAYRDRRQKKRDFRKLWITRINAAARMNGLSYSQLMHGLKLAEIEVNRKMLADLAVAD
                     AAAFTALADAAKAKLGK"
     gene            complement(5275..7485)
                     /locus_tag="VC122_001462"
     CDS             complement(5275..7485)
                     /locus_tag="VC122_001462"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012560599.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LTA synthase family protein"
                     /protein_id="HEP5641379.1"
                     /translation="MTSLGKNDKEVATVKKFKTLITGFINTRLGFIITLLFCYWIKTL
                     WAYHTDFSLDLGNIYQVFLTIINPIPLAFLLLGVALYVKNTRAFYICSWVVYIILNIL
                     LISNSIYYREFSDFITVSAMLASSKVSAGLGDSALNLLRIWDIIYILDFIILISLSIT
                     KKIKNDQRPFNKRAAFAITALSSLLLSINLFLAEIDRPELLTRGFSNTYIVRALGLPA
                     FTLYSGNQTYQAQKERNGATAEELIDVKTYVKGHYAAPDPQYFGIGKGKNIIVLHLES
                     FQQFLIDYKLKEGDKEYEVTPFINSLYHSNATLAFPNFFHQVKAGKTSDAETMMENSL
                     FGLNSGSFMVNYGGENTQFATPSILTQKGGYTSAVFHGNVGTFWNRNNAYKQWGYNYF
                     FDSSYFSKQNSKNSFQYGLNDKYMFKDSIKYLEQMQQPFYTKFITVSNHYPYTSLKGE
                     SSEEGFPLAKTDDETINGYFATANYLDAALKSFFDYLKATGLYENSIFVLYGDHYGIS
                     NSRNSSLAPLLGKDSETWSEYDNAMLQRVPYMIHIPGYTNGSIKETFGGEIDALPTLL
                     HILGIDTSQFVQLGQDLLSPQNSQIVAQRTSGTYMTPEYTNYSGRLYNTQTGLEITNP
                     DEMTIAKTKEIRSAVAQQLAASDAIQTGDLLRFDTQNGLKAIDPNQFIYTKQLKQLKD
                     ISAKLGSESTSLYSKNGHKSTQKLFKAPSYLELNPVEADAATSELKEDNPKNKE"
BASE COUNT         2465 a         1235 c         1498 g         2375 t
ORIGIN      
        1 tcagtttggc gcattaagct atggtataac gaaccttttc ccgctttatg tgtgtttctt
       61 cttttttgtt tatattagta atgctagtaa aataactatt aatattgtag aaaaaatggg
      121 tataatcttt gttatttcag cgattatagg tgcaactgta ttagctatgt acctgacttg
      181 gacaccagta gggtcctcaa ctgtcttagg tgttcaaagt cgttatctga taggaattat
      241 cccattagtg ttgcttcttt ttagtagtca acagcaaaag ttcaaacaaa tagaggatat
      301 cctttctgac aaactggcta ttcatgtttc gcttttgttt attctcgcaa tgttaatgag
      361 tactattttt agatattatc attaaactct taattagagg ctttaaaaaa cttagcttga
      421 ataagtattt taaagccttt tatgctataa ttaaattgag aaattattga ggtggcctat
      481 gaagtacgat aacttgttag atcgatttat aaaatatgtc aaagtgaata caagaagcga
      541 tccagacagt gagactactc ctagtacgga gagtcaagaa gcatttgcct taacgatttt
      601 aaagcctgaa atggaggcaa ttggtttgca agatgttcat tataatccag ttaatggcta
      661 tcttattgga actctgcctg ccaataatcc aaccttgacg cgtaaaattg gttttatagc
      721 acatatggat acggctgatt tcaatgctga aaatgttaat ccgcagatta ttgacaatta
      781 tcaaggaggg gacattacac ttggcagttc taattataag ttggatccaa aagcatttcc
      841 aaatcttaat aattatatcg gtcaaacatt gattaccact gatgggacta cacttttagg
      901 ggcagatgat aaatctggta ttgctgaaat catgacagca atagaattct taacatcaca
      961 accacaaata gagcactgtg acattaaagt ggcttttggc cctgatgaag agattggggt
     1021 cggagctgat aagtttgagg tggctgattt tgaggtggat tttgcttata ctatggatgg
     1081 cggtccttta ggcgaattgc aatatgaaac tttcagcgct gcagctctag aagtgacttt
     1141 tttaggcaga aatgttcatc ctggtacggc taaggatcaa atgattaatg cccttgagtt
     1201 agcaatagat tttcatgaga agctacctgc taaagagcga cctgaataca cagatggtta
     1261 tcaaggcttc tatcacttaa ccggactaac tggaacagtt gaagaggcac gcgcatctta
     1321 cattattcgt gattttgaag aagcatcatt tgaagctaga aaagtgaaag ttgaaaatat
     1381 agctcaatcg atgaatgctc agctaggaac aaaacgtgtc ttggtagagc ttaatgacca
     1441 atactacaat atgaaaaaag ttatcgaaaa agatatgact gctattgagt tagctaaaga
     1501 agtaatggaa gagcttgcta tcaagcctgt aatcgaaccc atacgtggag gaacagatgg
     1561 ctctaaaatt tcatttatgg gtattcccac tcctaatatt tttgcagggg gtgagaatat
     1621 gcacgggcgt tttgagtttg ttagcttaca aacgatggag agagctgtcg atgtcattat
     1681 tggacttgtt tgcaaagcct aattatcatg taattgatat ttagtggaag gggcttataa
     1741 aaatgattaa actatttggt aaaataaggt atcactggca gccagagttg tcttggtcta
     1801 ttatttattg gtctattgcg tttgcaccta tttttgtagg tttatctttg ttgtatgagc
     1861 gcactgaaat acctagtcgg gtttttattc tatttgctat ttttgctgtc ctagttggca
     1921 ttggattaca tcgttatttc attattgaaa ataatggtat tttaagaatc gtatctttta
     1981 aattgtttgg tccacgtaag ctacttattt ctactattac taagattgag gtgaccaaat
     2041 caacactttg tctgcacttt gaagataaaa gttatctctt ttatatgcgc aaatggccta
     2101 aaaaatattt tcttgatgcc ttagcggtta atccatactt ccaaggagaa gttatattaa
     2161 gtgataactt tattaaatta gactattttg aagtttatca gcatgataaa aaagccctta
     2221 ctcgagggta agagctttag ttgggcaaga tttgacagct aaaatagcgt ccttatctga
     2281 aggacaaatt gactgacttg tttcagatga gcttgaaaat gtgacgatac cattgtcatg
     2341 ataatcaaaa agtgatgaat aggtttggca tagaccacaa gcgatgcatt tttctggaat
     2401 gatagatact ttcataacta ttattctaat atgattttgt taaattaaca aggggggacg
     2461 atcatggcta aagaaccatg ggaagaaaaa attgttgatg atactatagg gacacgaaca
     2521 cgtaaatcaa gaaatgcttt cattagcacg ccttggttga ctgctttatt aagtgtattc
     2581 tttgtcatca ttgttgctat actttttatt ttcttttata catcaaatag cggtagtaat
     2641 agacaagctg aaacaaatgg tttttatgga gcatccactc ataaaaaaac aaggaaagct
     2701 tctaacgcta aaaaagcatc aagtagttca acaactacag acacaacacc ttctagcgaa
     2761 gaaacacttg cttctagtga aggaactggc gaaaccctta ctgtattggc aggtgagggg
     2821 gcagcttcta ttgcagcccg tgcaggtatt tctgtggagc agttacaagc acttaatcca
     2881 gagcacatga ctcaaggata ttggtatgcc aatccaggag atcaagtcac tattaaataa
     2941 ttgaagagga aaaaatgaaa gcaattaaaa ttgcgattga tgggccagct tcaagtggaa
     3001 aaagtacagt agccaagatt attgcaaaaa atcttggcta tacttatcta gatactggtg
     3061 ctatgtatcg ctcagcaact tatatcgcac tgacacatgg ctatacgggt aaagaagtag
     3121 cccttatttt agaggaactt gagaaaaatc ctatttcctt taaaaaagca aaagatggct
     3181 cccaattagt ttttcttggg gatgaggacg tgaccttagc cattaggcaa aatgatgtga
     3241 ctaacaatgt ttcttgggtc tcagctttgc cagaaattag agaagagctg gttcatcaac
     3301 aaagacgcat tgcacaagct ggtggcatta tcatggatgg tcgtgacatt ggaacggttg
     3361 ttctcccaga tgcggaacta aaaatctttt tagtggcttc tgtcgaagag cgggcagagc
     3421 gccgctataa agaaaaccta gaaaaaggaa ttgaatcaga ttttgagacg ctaaaagaag
     3481 agattgcagc tcgtgattac aaggatagtc atcgtaaggt ttcacctcta aaagcagctg
     3541 aagatgccct tatttttgat accacaggtg tttctattga tggcgtggtg caatttatcc
     3601 aagaaaaagc agaaaaaata gttgacatgt cctagagcct atgttagaat attaacaatg
     3661 agaaaagtag aagtgagagc ttctcgcctt gcgacttagg ttgtctggcc ctaacatgtc
     3721 aaattcctat ggaatattat gtgtgcgggc ttgatttatc aagtccgctt tcgtttttct
     3781 ctaaaaaaat aaagaggtga agatcatagc taaaaaggat ctattcatta atgatgaaat
     3841 tcgcgttcgc gaagttcgtc tcgttggtct tgaaggtgaa caattaggta ttaaaccatt
     3901 gtctgaagcg caatcattgg ctgatgcttc aaatgttgat ttggttttaa tccagccaca
     3961 agctgttcct cctgttgcca aacttatgga ctatggaaag ttcaaatttg agtatcaaaa
     4021 gaaacagaaa gaacaacgta aaaaacaaag cgttgtgact gtgaaagaag ttcgtctcag
     4081 tccagttatc gataaaggtg actttgaaac aaaacttcgt aacggtcgta aattccttga
     4141 aaaaggaaat aaagttaagg tttctattcg tttcaaagga cgtatgatta ctcataaaga
     4201 gattggtgcc aaggtactag ctgactttgc tgaagcgact caagatattg ctatcattga
     4261 gcaacgagca aaaatggatg gacgccaaat gtttatgcaa cttgcaccaa tttcagacaa
     4321 gaagtaattg tcacagtttc attatatctt taagaggaga atactaaaat gccaaaacaa
     4381 aaaacacacc gcgcatcagc taaacgtttt aaacgtacag gttcaggcgg tttgaaacgc
     4441 ttccgcgcct ttacatcaca ccgtttccac ggaaaaacta aaaaacaacg tcgtcatctt
     4501 cgtaaagctg gtttggttag ctcaggagat ttcaaacgta tcaaagcaat ggttactggt
     4561 ctttaatttt aaaggcagta gacatttacg agtattataa gaattatttg aaggagaact
     4621 attaatggca cgtgttaaag gtggcgttgt ttcacgcaaa cgtcgtaaac gtattttaaa
     4681 attagctaaa ggttactatg gagcaaaaca catcttgttc cgtactgcaa aagagcaagt
     4741 gatgaactct tactactatg cataccgtga tcgccgtcag aaaaaacgtg atttccgtaa
     4801 actatggatc actcgtatca atgcggctgc tcgtatgaac ggtttgtcat actcacaatt
     4861 gatgcacggt ttgaaacttg ctgaaatcga agtaaaccgt aaaatgcttg ctgatttagc
     4921 agtagctgat gcagcagctt tcacagctct tgctgatgca gccaaagcaa aacttggcaa
     4981 ataagaaacg ctatacctaa aaaataactt tctattacct atagaaagtt ataaagaaac
     5041 aaaaatgaag gagacgatgg acgtcttctt tttattatac tcaatataat aaaaagcagt
     5101 ttcccatgtt ttattaccag taatgtggga tatttagatg gtagcaagga gttttatagc
     5161 tgatttgttt tctttaggtc taattagcat attttgatta ctgataaact tgaatatcgc
     5221 caataaaaaa gaagcaaaat tattattttg cttctagtca gatcattaaa ttgattattc
     5281 tttatttttg gggttatcct cttttagttc agaagttgcc gcgtcagctt ctacgggatt
     5341 tagttctaag taagatggtg ctttaaaaag tttctgagtt gatttatgac catttttact
     5401 gtataaactt gttgactctg atccgagttt tgctgaaata tctttcagtt gttttaactg
     5461 cttagtgtag ataaactggt taggatcaat agcttttaga ccattttgag tatcaaagcg
     5521 caggagatca cccgtttgaa tagcgtcgct agctgctagt tgttgagcaa cagcagagcg
     5581 aatttcctta gttttggcta tagtcatttc atcgggatta gtgatttcta aacctgtttg
     5641 cgtgttgtac agacgtccac tatagttagt gtattcagga gtcatataag tcccagatgt
     5701 tcgttgtgcc acaatctggc tattttgagg tgataataag tcttgtccta attgaacaaa
     5761 ctgactagtg tcaataccaa gtatgtggag taaagtagga agagcatcga tttcaccgcc
     5821 aaaggtttct ttgatactac cattcgtata acctggaata tggatcatat acgggactcg
     5881 ttgtagcata gcattatcat attcagacca agtttcagaa tctttaccaa gaagtggagc
     5941 aagactagaa ttacgagaat ttgaaatacc ataatgatca ccatataaaa caaaaataga
     6001 attctcgtac aaaccagtgg ctttcaagta atcaaaaaaa gatttaaggg cagcgtctaa
     6061 ataattggca gtagcaaagt aaccattgat cgtttcatcg tctgttttgg ctaaaggaaa
     6121 accttcttca ctactttcgc cttttagact agtataagga tagtgattac tcacagtgat
     6181 aaacttggta taaaaaggtt gttgcatctg ttcaagatat tttatggaat ccttaaacat
     6241 gtacttatca tttaacccat attgaaaaga gttcttacta ttttgtttag agaagtagct
     6301 agaatcaaaa aaataattat agccccattg tttataagca ttattgcgat tccagaaagt
     6361 tccaacgtta ccatgaaaga cagcgctggt atagccacct ttttgggtta aaatacttgg
     6421 agtagcaaat tgtgtatttt caccaccgta gttcaccata aaagaaccac tatttaaacc
     6481 aaataaggaa ttctccatca tggtttcagc atcagaagtt ttacctgctt taacctgatg
     6541 aaaaaagtta gggaatgcta aggtagcatt tgagtgataa agcgagttga taaaaggtgt
     6601 tacctcatat tctttatctc cttcttttag tttataatct attaagaatt gttgaaagct
     6661 ttctaaatga agaacaataa tatttttgcc ttttccaatt ccaaaatact gaggatcagg
     6721 tgctgcataa tgtcctttga cataagtttt aacatcaatt aattcttctg cagtagctcc
     6781 atttcgttct ttttgagctt gataggtttg attgccgcta tagagggtaa acgctggtag
     6841 tcctaaagct ctcacaatat aagtatttga aaagccacgt gttaatagtt ccggacgatc
     6901 aatttctgct aaaaaaagat taatagaaag caatagacta gataaagcgg tgatggcaaa
     6961 tgctgcgcgc ttgttaaaag gacgttggtc atttttgatt tttttagtga tagataaact
     7021 aattagaata ataaaatcaa gaatatagat aatatcccag atgcgtaaaa ggtttaaagc
     7081 agagtcccct aatccagcgg agactttact actagctagc atggcactca ctgtaataaa
     7141 gtctgaaaat tctcggtaat atatagaatt tgatatcaag agaatgttaa ggataatata
     7201 aaccacccaa gaacagatat aaaaggctcg ggtattctta acgtaaagag caacccccaa
     7261 aagtaaaaaa gctagtggga ttggattgat aatagttaga aagacttggt atatatttcc
     7321 caaatctagt gaaaagtctg tatgatatgc ccacagggtc tttatccagt aacaaaagag
     7381 tagagtaatt ataaaaccca atcttgtgtt gataaaacct gtgataagtg ttttaaattt
     7441 cttcacagta gcaacttcct tatcgttttt tcctaaagat gtcatctcca aataaaatct
     7501 cagattaaat tataccaaaa atgtgaagct aatgcttgtt gtaagttcaa atttagtagg
     7561 attttttatc aga
//
LOCUS       DATXIG010000062         7487 bp    DNA     linear   BCT 28-DEC-2023
DEFINITION  TPA_asm: Streptococcus pyogenes strain MGAS11113
            SAMN08555799-rid19682583.denovo.062, whole genome shotgun sequence.
ACCESSION   DATXIG010000062 DATXIG010000000
VERSION     DATXIG010000062.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN08555799
            Sequence Read Archive: SRR6740495
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pyogenes
  ORGANISM  Streptococcus pyogenes
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 7487)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7487)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-OCT-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-OCT-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001918058.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001918058.2
            Genome Coverage       :: 368x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/12/2023 11:23:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 1,806
            CDSs (total)                      :: 1,769
            Genes (coding)                    :: 1,722
            CDSs (with protein)               :: 1,722
            Genes (RNA)                       :: 37
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1, 1 (5S, 23S)
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 47
            CDSs (without protein)            :: 47
            Pseudo Genes (ambiguous residues) :: 0 of 47
            Pseudo Genes (frameshifted)       :: 30 of 47
            Pseudo Genes (incomplete)         :: 20 of 47
            Pseudo Genes (internal stop)      :: 9 of 47
            Pseudo Genes (multiple problems)  :: 11 of 47
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7487
                     /organism="Streptococcus pyogenes"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN08555799-rid19682583.denovo.062"
                     /strain="MGAS11113"
                     /serotype="Emm28"
                     /isolation_source="normally sterile site"
                     /host="Homo sapiens"
                     /db_xref="taxon:1314"
                     /country="Finland"
                     /collection_date="2002"
                     /collected_by="National Public Health Laboratory"
     gene            98..346
                     /locus_tag="VC122_001463"
     CDS             98..346
                     /locus_tag="VC122_001463"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002984469.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GlsB/YeaQ/YmgE family stress response membrane
                     protein"
                     /protein_id="HEP5641380.1"
                     /translation="MGLIWTLIVGALIGVIAGALTKKGGSMGWIANIAAGLVGSSVGQ
                     ALLGSWGPSLAGMSLIPSVIGAVIVVMITSFVLNKTNN"
     gene            383..994
                     /gene="amaP"
                     /locus_tag="VC122_001464"
     CDS             383..994
                     /gene="amaP"
                     /locus_tag="VC122_001464"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011106783.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alkaline shock response membrane anchor protein
                     AmaP"
                     /protein_id="HEP5641381.1"
                     /translation="MLSRKEVCMSKLLKISYCLVGLVLLSVFGWVVGITGGYIYLPYS
                     YRWLSWGMDSFPNLLDSALSYYYFWTALVLFVITFLALLVIILYPRIYTEVQLRHKNK
                     KGTLLLKKSAIESYVATAIQTAGLMPNPTVTAKLYKRKFKIIVKGRLASRVAVADQIS
                     GVKEGIEKGLTEFFGINYPVNFKVYVKDIADSDRKHITRNRVE"
     gene            1005..1193
                     /locus_tag="VC122_001465"
     CDS             1005..1193
                     /locus_tag="VC122_001465"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011888836.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2273 domain-containing protein"
                     /protein_id="HEP5641382.1"
                     /translation="MEFYEKFKYPIIGGLVGLIIAILLMAFGLFKTLLAIIFIILGIY
                     GGLYAKKTGIIDQFLNRK"
     gene            1206..1745
                     /locus_tag="VC122_001466"
     CDS             1206..1745
                     /locus_tag="VC122_001466"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015632041.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Asp23/Gls24 family envelope stress response
                     protein"
                     /protein_id="HEP5641383.1"
                     /translation="MTETYIKNTSKDLTSAIRGQLTYDDKVIEKIVGLALENVDGLLG
                     VNGGFFANLKDKLVNTESVRDGVNVEVGKKQVAVDLDIVAEYQKHVPTIYDSIKSIVE
                     EEVKRMTDLDVIEVNVKVVDIKTKEQFEAEKVSLQDKVSDMARSTSEFTSHQVENVKA
                     SVDNGVEKLQDQKAEPRVK"
     gene            1786..1986
                     /locus_tag="VC122_001467"
     CDS             1786..1986
                     /locus_tag="VC122_001467"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011528688.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CsbD family protein"
                     /protein_id="HEP5641384.1"
                     /translation="MSDEKYNAKLDQAGGKLKEGFGKISGDKSLETEGKVDKVTGKVK
                     EVIADAKDTVKGLAKGLDNKDK"
     gene            1997..2485
                     /locus_tag="VC122_001468"
     CDS             1997..2485
                     /locus_tag="VC122_001468"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011017825.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Asp23/Gls24 family envelope stress response
                     protein"
                     /protein_id="HEP5641385.1"
                     /translation="MEKTQIKSTLTYDDKVIEKIVGHALENVGGLLAVTGGFFSNIKN
                     NLVNSESVTDGVSVEVGSKEVAVDLAIIVEYGKDIPAIAESIKAIVSQNVDSMTHLKV
                     VEVNVNVVDIRTKEEHEAASVTVQDRVTNAASSTSQFVSEQTEKLKDTISDTVNSDEA
                     AK"
     gene            2648..3289
                     /locus_tag="VC122_001469"
     CDS             2648..3289
                     /locus_tag="VC122_001469"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010922367.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HEP5641386.1"
                     /translation="MISPKKEITSSKYQKIAISVAQRIANGEYEVGEKLKSRTTIAST
                     FNVSPETARKGLNILADLKILTLKHGSGAIVLSKERAIEFINQYESTHSIAVLKEKIR
                     ETINDQGKAMEKMAVLVNDFLMQSQSVSKQYPLAPYEIICSQDSEHFGKSIGVLNIWH
                     QTGATIVAIEHAGQFIVSPGPYSVIEKGDHIYFVGDESVISRMKTFFNLRKGL"
     gene            complement(3432..3974)
                     /locus_tag="VC122_001470"
     CDS             complement(3432..3974)
                     /locus_tag="VC122_001470"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012560750.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HEP5641387.1"
                     /translation="MTKRHTETKAYVKTALTTLLTEQSFETLTVSDLTKKAGINRGTF
                     YLHYTDKFDMMNHFKNDTLDDLYRLLNQAEIYTDTRQVLNQTLSYLIEHREFITALAT
                     ISYLKFPQLIKDFCYQFLTTITGFQDIVTNQYHIPYPYALEVYLASIESIISYWIANG
                     YQETTEEVADIILKAVTLDN"
     gene            4092..4793
                     /locus_tag="VC122_001471"
     CDS             4092..4793
                     /locus_tag="VC122_001471"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011017822.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEP5641388.1"
                     /translation="MAFIELKQVSKSYQVGETTVFANHEVSFEINKGELVVILGASGA
                     GKSTVLNILGGMDTVDAGQVIIDGKDIAHYTSKALTQYRRNAIGFVFQFYNLVPNLTA
                     KENVELAVEIVADALDPVTILKEVGLSHRLDHFPAQLSGGEQQRVSIARALAKNPKLL
                     LCDEPTGALDYQTGKQILTLLQDMARTKGTTVVIVTHNAAIAPIADRVIFMHDAQVTK
                     TVINKEPASIETIDY"
     gene            4808..7444
                     /locus_tag="VC122_001472"
     CDS             4808..7444
                     /locus_tag="VC122_001472"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014407618.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FtsX-like permease family protein"
                     /protein_id="HEP5641389.1"
                     /translation="MIKKTLWKDILRAIKNSKGRFISLFFLMALGSFALVGLKVTGPD
                     MERTASRYLERHQVMDLTVLASHQFSQADKQELDTLKGAHLEYGHLLDVSLTSNQKSL
                     RLYSVPKKVSKPVLVKGSWSKRETDLVLSSSLAKNYQIGDELAVTSPMEGLLTTTHFQ
                     VVGFANSSEVWSKSNLGSSSTGDGSLYAYAFVNPNVFKSAFNLLRIRFSNLRLTNAFS
                     KDYQKRVTQNQAHLDNLLKDNGQKRYDDLQNQYDLALKNGRVALAKETVKLAASEENL
                     TFLEGSALQEAKHQIEQGKQALAKEEKQLEQVQATKDKLEKPSYLTYNRSTLPGGEGY
                     HTYATSTTSISNVGNIFPVVLYLVAALVAFTTMTRYVDEERTSSGLLKAIGYSNKDIS
                     LKFLIYGLLASFLGTTLGIIGGTYLLSALISEILTGALTIGKTHLYSYWFYNGIAYLL
                     AMLSAVLPAYLIVKKELFLNAAQLLLPKPPSKGAKIWLEHLTFVWKALSFTHKVTIRN
                     IFRYKQRMLMTIVGVAGSVALLFAGLGIQSSLAKVVEHQFGDLTTYDILAVGSAKATA
                     TEQTDLASYLKQEPITGYQKVSYASLTLPVKGLPDKQSISILSSSATSLSPYFNLLDS
                     QEQKKVPIPTSGVLISEKLASYYKVKPGDQLVLTDRKGQSYKVTIKQVIDMTVGHYLI
                     MSNTYFKNHFKGLEAAPAYLIKVKDKDSKHIKETASDLLTLKAIRAVSQNANHIKSVQ
                     LVVTSLNQVMTLLVFLSILLAIVILYNLTTINIAERIRELSTIKVLGFYDQEVTLYIY
                     RETISLSLVGILLGIYLGKGLHTYIMTMISTGDIQFGVKVDAYVYLVPILVILSLLAV
                     LGIWVNRHLKKVDMLEALKSID"
BASE COUNT         2364 a         1215 c         1545 g         2363 t
ORIGIN      
        1 ttgaaagtcg actattaaaa gactattgct tgaagaataa cagctgttat tcttccttgg
       61 acaaaaggta caggagtatc taaaaaggag aattatcatg ggacttattt ggactttaat
      121 cgtcggtgcg cttattggtg tgattgctgg agcacttact aaaaaaggcg gttcaatggg
      181 ctggattgca aacatcgctg ctggtctagt tggttcttcc gttggtcaag ctttacttgg
      241 ttcatggggc ccttctttag caggaatgtc tttgatcccg tcagttattg gtgccgttat
      301 cgttgttatg atcacttctt ttgtactaaa caaaactaac aactagtttc ctctttattt
      361 agtaagtact tacaaataga gaatgttaag tagaaaagag gtctgtatgt caaaattgtt
      421 aaagattagt tattgtcttg tcggacttgt tctattgtct gtttttggat gggtagttgg
      481 aataaccgga ggctatatat atttacctta tagttatcgt tggctaagtt ggggtatgga
      541 tagttttccc aatctcttag attcagcttt atcctattat tacttctgga cagcccttgt
      601 attatttgtt ataacatttc ttgccttatt agttattatt ttatacccaa gaatttatac
      661 tgaggtccag ttacgccata aaaataaaaa aggaacactt ttattaaaaa aatcggctat
      721 tgaaagttat gttgcgaccg ctattcaaac tgcaggtttg atgccaaacc caacagtcac
      781 agctaaatta tataagcgga agtttaagat tatcgttaaa ggacgtttag cttctcgtgt
      841 agcagtggca gatcaaatta gtggcgtgaa agaagggatc gaaaaaggtt taaccgagtt
      901 cttcggtatt aattatcctg ttaatttcaa agtttatgtt aaagatattg ctgattctga
      961 tcgtaaacat attaccagaa atcgtgtaga ataggagatc tatcatggaa ttttatgaaa
     1021 aattcaagta ccctattatt ggtggcttgg tggggctaat tattgccata ttgctcatgg
     1081 cttttggttt attcaaaacg ctactagcaa ttattttcat tattttagga atatatggtg
     1141 gcctgtatgc caaaaaaaca ggtattattg atcaatttct taatcgcaaa taaaggagac
     1201 ttattatgac tgaaacttat attaaaaaca catctaaaga cttaacttca gcaattcgtg
     1261 gtcaattaac atatgatgat aaagtaattg aaaaaattgt tggtttagca cttgaaaatg
     1321 ttgatgggtt actgggtgta aatggtggtt tttttgctaa cctaaaagat aaattagtaa
     1381 acaccgaatc tgtacgtgat ggcgtgaacg tggaagttgg aaaaaaacaa gttgccgttg
     1441 atcttgatat tgtcgctgaa tatcaaaagc atgtgccgac tatttatgac tctattaaat
     1501 cgatcgttga agaagaagtg aagagaatga ccgatctgga tgtgattgaa gttaatgtta
     1561 aagtagtcga cattaaaaca aaagaacaat ttgaagctga aaaagtaagt ttacaagaca
     1621 aagtttcaga catggcacgt tcaacatctg aatttacgag tcatcaagtt gagaatgtta
     1681 aagcttcagt ggataacggt gttgaaaaac ttcaagatca aaaagctgag ccacgtgtaa
     1741 aataatcata tgtcgtcaat taggtaagct aaaggagggt ttattgtgtc agatgaaaaa
     1801 tataatgcga aattagatca agctggtggc aagttaaaag aaggatttgg taagatttct
     1861 ggagataaat ctttagaaac tgaaggtaaa gtagataaag ttactggaaa agttaaagag
     1921 gttattgcag acgccaaaga tactgtaaaa ggattagcta aaggcttaga taataaagat
     1981 aaataaaggg gacatgatgg aaaaaactca aattaaaagc actcttactt atgacgataa
     2041 ggtcatcgaa aaaattgtcg gtcatgcttt ggaaaacgtt ggcggattac tagctgtaac
     2101 aggtggattt ttctcaaata ttaaaaataa tttggtgaat tctgagtctg taactgatgg
     2161 tgtttcagtt gaagttggta gcaaggaagt agcagttgat ttagcaatca ttgtagaata
     2221 cggtaaagat attcctgcta ttgcagaatc tattaaagct attgtttctc aaaacgttga
     2281 tagtatgaca catttgaaag ttgttgaagt caatgtgaac gttgttgata ttcgtactaa
     2341 ggaagaacat gaagctgcta gcgttactgt tcaagatcgt gtcactaatg cagcttcaag
     2401 tacttcacaa tttgtatcag aacaaacgga aaaattaaaa gatactatct ctgatactgt
     2461 aaatagtgat gaggcagcta aataagcaat cgtctaggaa gtattattga ctagtggtga
     2521 taagtttgag ctggtattta ccagctcttt ttgtcacggt taaaaagaca cggactattg
     2581 tcatctctct aaaaatatga tttaattaga tagaaatttt tcaaattgtt agaaaggagt
     2641 aaattaagtg ataagtccta aaaaagagat aacaagttca aaatatcaaa aaattgctat
     2701 ttcagtagct cagcgtatcg caaatggcga atatgaagtg ggcgaaaagt taaagtcaag
     2761 aactactatc gcctcgacat ttaatgtatc accagaaacg gctcgtaaag gcctgaatat
     2821 tttagctgac ttgaaaattt tgactttaaa acatggtagt ggagctattg tgctgtcaaa
     2881 agaaagagct attgagttta taaatcaata cgaatcaacc cattccatcg ctgttttaaa
     2941 agaaaaaata cgtgagacta ttaatgatca gggaaaagca atggaaaaaa tggctgtttt
     3001 ggtcaatgat tttttaatgc aaagtcaatc tgttagtaag caatacccat tagcacctta
     3061 tgagattatt tgtagtcaag attcggaaca ttttggcaaa tctattggtg tgttaaatat
     3121 ctggcaccag acaggagcaa ctatcgtcgc tattgaacat gctggtcagt ttatcgtttc
     3181 accaggtcct tattctgtta ttgaaaaagg cgatcatatt tatttcgttg gtgatgagtc
     3241 agtcatttca cgaatgaaga ccttttttaa tttacgaaaa ggattataag tttgagggcc
     3301 cacctcaaac tttttttgtt gctgttgagc attattaaaa gaggcagatc atcatggttt
     3361 gtctttccaa aatatctgtt tagacagttg ttttggattg ggagcatgat ttcagactct
     3421 tttgatgttt tttaattgtc tagagtaact gctttaagaa taatatcggc aacctcttca
     3481 gtggtttctt gatagccatt agcaatccaa tagctgataa tgctctcaat actggcaagg
     3541 taaacttcta aagcataggg ataaggaata tggtattgat tggtcacaat gtcttggaaa
     3601 ccagtaatag tagtcaaaaa ttggtaacag aaatccttga tgagttgcgg aaatttgagg
     3661 taagaaatag tagcgagtgc ggttataaat tctctatgtt ctataaggta ggacaaggtt
     3721 tgattgagga cttgtcgagt gtctgtgtag atttcagcct gatttaacaa acggtacaaa
     3781 tcatccagag tgtcattttt gaaatgattc atcatgtcaa atttatcagt gtagtggagg
     3841 tagaaggttc ctctattgat accagccttt ttagtaaggt cagagacagt aagagtttca
     3901 aaagattgtt cagttagtaa ggtggttaag gcggtcttta cataagcttt ggtttcagta
     3961 tgtcttttgg tcattctcag gtgctcctta aatcaacagt tgcgtcagat ttgttcttgt
     4021 taaatactta tggccattat ataataaggc tataattaaa tcaacactat gttcaaataa
     4081 tggaggggca gatggctttt attgaattaa aacaggtttc aaaatcttat caggtaggag
     4141 agacgactgt ttttgccaac cacgaagtgt cttttgagat taataaagga gaactagtgg
     4201 ttattttggg agcatccgga gctggtaaat cgacagtcct caatattttg gggggcatgg
     4261 acacagtaga tgcaggtcaa gtaattattg atggcaaaga cattgctcat tacacgtcta
     4321 aggccttaac tcagtatcgt cggaatgcta ttggtttcgt ttttcaattt tataatttgg
     4381 ttcctaattt gacagctaaa gaaaatgttg aattagcagt tgaaattgta gcagatgctt
     4441 tagatcctgt gaccatttta aaggaagtag gactcagtca tcgtctggat cattttcctg
     4501 ctcagctctc aggtggtgaa cagcaacggg tttcgatagc acgtgcctta gctaaaaacc
     4561 ctaaattgct tctttgtgat gaacctacag gtgcccttga ctaccaaaca ggaaagcaaa
     4621 ttctaaccct cttacaggat atggcacgaa ctaaggggac cacggtagtt attgtaaccc
     4681 acaatgctgc tattgctccg atagctgatc gtgtcatttt tatgcatgat gcacaggtta
     4741 ccaagacagt cattaataag gaacctgcaa gcattgagac aattgattat taagaggaag
     4801 gtgtcgtatg ataaagaaaa ccttatggaa ggatattttg agagctatca agaatagtaa
     4861 agggcgattc atttccttat ttttcctaat ggccttaggc tcctttgctt tagttggact
     4921 taaagtcacc ggtccagata tggaacgaac agccagccgt tacctcgaaa gacaccaggt
     4981 gatggaccta acggtactgg cttctcatca attttcccaa gccgataaac aagagttaga
     5041 tacgttaaaa ggggctcatt tagaatatgg tcatttactt gatgtcagtc taactagcaa
     5101 ccagaaatct cttaggctct atagcgtgcc aaagaaagtg tctaagccag tcttggttaa
     5161 agggagttgg tcaaagcgag agacagattt ggttttatcc tcctcacttg ctaaaaacta
     5221 tcagattgga gatgaactag cagtcacctc acctatggaa ggtttgctga cgacaaccca
     5281 ttttcaagtg gttggttttg cgaattcttc agaagtttgg tccaagtcca atttaggaag
     5341 ttcctcaacc ggagatggaa gtctctacgc ttatgctttt gttaatccaa acgtttttaa
     5401 aagtgccttt aacctgctgc gcatccgttt tagtaatctt cgtctgacaa acgctttttc
     5461 caaagactat caaaaaaggg taacccaaaa ccaagctcat ttggataatc tacttaaaga
     5521 taatggccag aagcgttatg atgacctcca aaatcagtat gaccttgcct taaaaaatgg
     5581 cagagtagca cttgcaaagg aaacagtgaa actagctgcg agtgaggaga acttaacttt
     5641 tttagaaggc tctgctttac aagaagctaa gcatcagatt gaacaaggca aacaagcatt
     5701 agccaaggag gaaaagcagt tagagcaggt gcaggctaca aaagataagc tagaaaaacc
     5761 cagttacctg acttataatc gctcgaccct accaggagga gaaggatatc atacttatgc
     5821 aacttcaacg acctccattt caaatgttgg aaatattttt cctgttgttc tttatctcgt
     5881 agctgctcta gtagccttta ccaccatgac acgttatgtt gatgaagaaa gaacaagctc
     5941 tggtctatta aaagccattg gttattctaa caaggatatc agtttaaagt ttcttatcta
     6001 tgggctttta gccagttttt tagggacaac tttaggtatt attgggggaa cttacctctt
     6061 atctgccttg atttcagaga tcttaacagg agctttgact attggaaaga ctcaccttta
     6121 tagttattgg ttttataatg gcatagctta cttgctggct atgttatctg ctgttttacc
     6181 cgcctactta attgtcaaaa aggaattatt cctcaatgca gctcagttat tgctgcccaa
     6241 acctcctagt aagggggcaa aaatctggtt ggaacacctt acttttgtct ggaaagccct
     6301 gtcctttact cacaaggtga ccatacgtaa tatttttcgc tataaacaaa gaatgctgat
     6361 gaccattgta ggcgttgcag gctcagtagc ccttttattt gcaggcttag ggattcagtc
     6421 gtcattagcc aaagtagttg agcatcaatt tggtgattta acgacttatg atattttggc
     6481 tgtcggttcg gccaaagcga cagcgacaga gcaaactgac ttagctagct atcttaaaca
     6541 agaacctatt acagggtacc aaaaggtatc ttatgccagc ttaacccttc ctgtaaaggg
     6601 attacctgat aagcaaagta tttccatttt atcaagttca gctacttctc ttagccccta
     6661 ttttaatctg ctggatagtc aggagcaaaa gaaggttccc attccaacct ctggtgtttt
     6721 gatttctgag aaattagcct cctattacaa ggtaaaacca ggtgatcagt tggtattgac
     6781 tgatcggaaa ggacagtctt ataaagtgac gattaaacag gttattgaca tgacagttgg
     6841 ccattacctg ataatgtcta atacctattt taagaatcat tttaaaggat tggaggctgc
     6901 tcctgcctat ctgattaagg taaaagacaa agatagcaag cacataaagg agacagccag
     6961 tgacttgtta accttaaaag cgattagagc agtttcacaa aacgccaatc atattaaatc
     7021 tgttcagcta gtagtcacct ctcttaatca ggtcatgacc ctccttgtct tcttgtctat
     7081 tttattagca atcgttatcc tttataactt aacgactatt aatattgctg agcgtattcg
     7141 agaattatcc actattaaag ttctgggatt ttacgatcag gaggtcacct tatatattta
     7201 tcgagaaact atttcgctat ccctagtagg cattctttta ggtatctatt taggaaaagg
     7261 cctgcatact tatatcatga caatgatttc aactggggat attcaatttg gtgtaaaggt
     7321 tgatgcttat gtttatctag tgccaattct agtaatcctt agcttgttag cggtattagg
     7381 tatctgggtt aatcgccatt taaaaaaggt tgatatgtta gaagctttga aatccataga
     7441 ttgattggtg aaaagacaga tatagacagc acttgaaaag ttaatca
//
LOCUS       DATXIG010000063         7481 bp    DNA     linear   BCT 28-DEC-2023
DEFINITION  TPA_asm: Streptococcus pyogenes strain MGAS11113
            SAMN08555799-rid19682583.denovo.063, whole genome shotgun sequence.
ACCESSION   DATXIG010000063 DATXIG010000000
VERSION     DATXIG010000063.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN08555799
            Sequence Read Archive: SRR6740495
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pyogenes
  ORGANISM  Streptococcus pyogenes
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 7481)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7481)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-OCT-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-OCT-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001918058.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001918058.2
            Genome Coverage       :: 368x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/12/2023 11:23:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 1,806
            CDSs (total)                      :: 1,769
            Genes (coding)                    :: 1,722
            CDSs (with protein)               :: 1,722
            Genes (RNA)                       :: 37
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1, 1 (5S, 23S)
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 47
            CDSs (without protein)            :: 47
            Pseudo Genes (ambiguous residues) :: 0 of 47
            Pseudo Genes (frameshifted)       :: 30 of 47
            Pseudo Genes (incomplete)         :: 20 of 47
            Pseudo Genes (internal stop)      :: 9 of 47
            Pseudo Genes (multiple problems)  :: 11 of 47
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7481
                     /organism="Streptococcus pyogenes"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN08555799-rid19682583.denovo.063"
                     /strain="MGAS11113"
                     /serotype="Emm28"
                     /isolation_source="normally sterile site"
                     /host="Homo sapiens"
                     /db_xref="taxon:1314"
                     /country="Finland"
                     /collection_date="2002"
                     /collected_by="National Public Health Laboratory"
     gene            27..878
                     /locus_tag="VC122_001473"
     CDS             27..878
                     /locus_tag="VC122_001473"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010922244.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR00159 family protein"
                     /protein_id="HEP5641390.1"
                     /translation="MNNLSSIDIKFLLSLFADPWLLAVHLLDILIVAYLIYRFIKALT
                     GTKIMSLVQGVIFFLVLRFIAEWIGFTTITYLMNQVITYGVIAGVVIFTPEIRAGLEK
                     FGRSTQVFLQKQYVSSESALVDALIKSVAYMGPRKIGALIAIEQTQTLQEYIATGIPL
                     NADISSQLLINIFIPNTPLHDGAVIVGQNKIVAACAYLPLSESKAISKEFGTRHRAAI
                     GLSENSDALTIIVSEETGAISVTRKGQFLHDLSTDEFETVLRTYLMSDSNVSLPWYKK
                     ILGGKSK"
     gene            875..1831
                     /locus_tag="VC122_001474"
     CDS             875..1831
                     /locus_tag="VC122_001474"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002984857.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YbbR-like domain-containing protein"
                     /protein_id="HEP5641391.1"
                     /translation="MKRFLNSRPWLGMVSVFFAILLFLTAASSNHNNSSSQIYSPIET
                     YTHSLKDVPIDMKYDSDKYFISGYSYGAEVYLTSTNRIKLDSEVNNDTRNFKIVADLT
                     HSHPGTVNVNLRVENLPSGVTATVSPDKISVTIGKKESKVFPVRGSVDAKQIANGYEI
                     SKIETGVNKVEVTSDESTIALIDHVVAKLPDDQVLDRNYSSRVTLQAVSADGTILASA
                     IDPAKTNLSVAVKKITKSVPIRVEAVGMMDDSLSDIQYKLSKQTAVISGSREVLEDID
                     EIIAEVNISDVTKNTSKTVSLSSSQVSIEPSVVTVQLTTTKK"
     gene            1885..3240
                     /locus_tag="VC122_001475"
     CDS             1885..3240
                     /locus_tag="VC122_001475"
                     /EC_number="5.4.2.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011285502.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoglucosamine mutase"
                     /protein_id="HEP5641392.1"
                     /translation="MGKYFGTDGVRGEANVELTPELAFKLGRFGGYVLSQHETERPKV
                     FVARDTRISGEMLESALIAGLLSVGIEVYKLGVLATPGVSYLVRTEKASAGVMISASH
                     NPALDNGIKFFGNDGFKLADDQELEIEALLDAPEDTLPRPSAEGLGTLVDYPEGLRKY
                     EKFLVTTGTDLSGMTVALDTANGAASVSARDVFLDLNAEIAVIGEKPNGLNINDGVGS
                     THPEQLQELVKETGADLGLAFDGDSDRLIAVDETGEIVDGDRIMFIIGKYLSEKGLLA
                     HNTIVTTVMSNLGFHKALDKQGINKAITAVGDRYVVEEMRSSGYNLGGEQSGHVIIMD
                     YNTTGDGQLTAIQLAKVMKETGKSLSELAAEVTIYPQKLVNIRVENSMKDRAMEVPAI
                     ANIIAKMEDEMAGNGRILVRPSGTEPLLRVMAEAPTDAEVDYYVDTIADVVRTEIGCD
                     N"
     gene            3364..4005
                     /locus_tag="VC122_001476"
     CDS             3364..4005
                     /locus_tag="VC122_001476"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014407536.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEP5641393.1"
                     /translation="MTKQQIEKLADLLPPFAATLYLDMIPEFQEGYASYVYQTPKQDI
                     QKRRIKQLTKNFERLLDTPSFYVMPVTRKTAEQLLADKGLAYHLLSTVAANQVFAAAV
                     NEDQTLFQVLRNGARVAFVILQEKGEFLEMTLGILETVTENEVPAAIVEAVQTYLLAD
                     MQENLPSLSIMVSQESSKLFYQEQGFVLLAKGCKPGNENDSDVCQLVKYFSRP"
     gene            4068..5198
                     /locus_tag="VC122_001477"
     CDS             4068..5198
                     /locus_tag="VC122_001477"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015058522.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oxygen-independent coproporphyrinogen III
                     oxidase"
                     /protein_id="HEP5641394.1"
                     /translation="MSKKPTSAYVHIPFCTQICYYCDFSKVFIQNQPVDAYLKALIQE
                     FDSYGIRDLKTLYIGGGTPTAITAKQLEYLLNHLERNLNLDDLEEFTIEANPGDLTPE
                     KIAVLQRSAVNRISLGVQTFNNKQLKQIGRSHNEEQIYSTIANLKTAGFHNISIDLIY
                     ALPGQTLDQVKENVAKALALDIPHLSLYSLILEHHTVFMNKMRRGKLNLPTEDLEAEM
                     FEYIISEMEANGFEHYEISNFTKPGFESRHNLMYWDNVEYFGCGAGASGYLNGIRYQN
                     RVPIQHYLKAVEAGNARLNEEVLRKEEMMEEELFLGLRKKTGVSIQRFQEKFGVSFEE
                     RYGNIVRELQNQGLLVKDDAFVRMTKKGLFLGDSVAERFILD"
     gene            5208..5960
                     /locus_tag="VC122_001478"
     CDS             5208..5960
                     /locus_tag="VC122_001478"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002984853.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-[acyl-carrier-protein] thioesterase"
                     /protein_id="HEP5641395.1"
                     /translation="MGLSYQEELTLPFELCDVKSDIKLPLLLDYCLMVSGRQSAQLGR
                     SNNNLLVDYKLVWIVTDYEITIHRLPHFQETITIETKALSYNKFFCYRQFYIYDQEGG
                     LLVDILAYFALLNPDTRKVATIPEDLVAPFKTDFVKKPHRVPKMPLLEQSIDRDYYVR
                     YFDIDMNGHVNNSKYLDWMYDVLGCEFLKTHQPLKMTLKYVKEVSPGGQITSSYHLDQ
                     LTSYHQITSDGQLNAQAMIEWRAIKQTESETD"
     gene            5960..6724
                     /locus_tag="VC122_001479"
     CDS             5960..6724
                     /locus_tag="VC122_001479"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003054515.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR01457 family HAD-type hydrolase"
                     /protein_id="HEP5641396.1"
                     /translation="MPYKGYLIDLDGTIYQGKNRIPAGERFIKRLQERGIPYLLVTNN
                     TTRTPEMVQSMLANQFHVETGIETIYTATMATVDYMNDMNRGKTAYVIGETGLKSAIA
                     AAGYVEELENPAYVVVGLDSQVTYEMLAIATLAIQKGALFIGTNPDLNIPTERGLMPG
                     AGALNALLEAATRVKPVFIGKPNAIIMNKSLEVLGIQRSEAVMVGDNYLTDIMAGIQN
                     DIATILVTTGFTRPEEVPTLPIQPDHVLSSLDEWRL"
     gene            6724..7356
                     /locus_tag="VC122_001480"
     CDS             6724..7356
                     /locus_tag="VC122_001480"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014407540.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR01906 family membrane protein"
                     /protein_id="HEP5641397.1"
                     /translation="MVENTKLLCSWVWLLALAILITIYSTWLWYPLEVDHLKLEQVVF
                     MSKDAILHNYNGLLNYLTNPFVTRLEFANFHSSADGLKHFADVKWLFHLTQVVFLGLL
                     YPTLKTFTQRLKTKRFWLLQKPLILAALFPLMIGLMASFIGFEHFFTLFHQVLFVGDS
                     SWLFDPLKDPVIWILPEVFFLHCFLFFMIVYEIILWSLVGLARWQRLNVE"
BASE COUNT         2278 a         1320 c         1602 g         2281 t
ORIGIN      
        1 ttttttgata tagaaatgag agttgtatga ataatttatc tagtatcgat attaaatttt
       61 tattaagctt atttgcggat ccttggctgc tggcagtgca tcttttagat attctaattg
      121 ttgcctattt gatttatcgt tttattaaag cattgactgg aactaaaatc atgtctttag
      181 ttcaaggggt cattttcttt ttggttcttc gttttattgc agagtggatt ggctttacga
      241 caatcacata cttaatgaac caagtcatta cttatggtgt aattgcagga gtagtcattt
      301 ttacccctga aattcgggca ggactggaga aatttggtcg ttctacgcaa gtttttttac
      361 aaaaacagta cgtcagcagt gaatctgctt tggtcgatgc tttgattaaa tctgtggcct
      421 atatgggtcc acgcaagata ggtgcattga ttgctattga acagactcaa accttacaag
      481 aatatattgc gacaggaatt ccgctgaatg ccgacatttc aagtcaacta ctcatcaata
      541 tttttattcc gaatacgccg cttcatgatg gagctgttat cgttggtcag aataagattg
      601 tggcagcctg tgcttacttg cctttgtcag aatcaaaggc aatttccaaa gaatttggga
      661 cccgtcatag agcggctatt ggattatctg aaaactctga tgcactaact atcattgttt
      721 cagaagaaac aggagctatt tcagtaactc gcaaaggcca atttttacat gatttaagca
      781 cagatgaatt tgaaacggta cttaggacct acctgatgag tgattcaaat gttagtttac
      841 catggtataa gaaaattcta ggagggaaat ctaaatgaaa cgatttttaa atagtcgtcc
      901 ttggttagga atggtatcag tcttttttgc gattctcctt tttttaactg cagcatcgag
      961 caatcataat aattctagct cgcaaattta tagcccgatt gaaacctata cgcatagcct
     1021 taaagatgta ccaattgata tgaagtatga tagtgacaaa tattttatca gtgggtattc
     1081 ttatggtgcg gaagtttatc taacctccac taatcgtatc aaattagatt ctgaagtgaa
     1141 caatgataca cgtaatttta agattgtggc ggatctaaca catagtcatc caggtacagt
     1201 aaatgtcaac ctaagagtag aaaaccttcc ttctggggtg acagctaccg tatctccaga
     1261 taaaatatca gtcacaattg gcaaaaaaga atctaaggta tttcctgtta gaggaagcgt
     1321 tgatgctaaa caaattgcaa atggttatga aattagcaaa attgaaacgg gggttaataa
     1381 ggttgaagtg accagtgatg aatctaccat tgctttaatt gatcacgttg tggcaaagtt
     1441 acctgatgat caagtgttag ataggaatta tagtagtcga gtgaccttac aagctgtctc
     1501 agctgatgga actattttag caagtgcaat tgatcctgca aaaacaaatt tatcagttgc
     1561 tgtaaaaaaa ataacgaaat cagtcccgat tagagttgag gcagtgggga tgatggatga
     1621 tagcttatca gatattcaat acaaattgtc aaaacaaaca gctgttatct caggtagtcg
     1681 agaggtctta gaagatattg atgaaattat tgcagaagtt aacatttctg atgttactaa
     1741 aaatactagt aagaccgtga gtttgtcttc tagtcaggtg tcaattgagc cgtcagtagt
     1801 gaccgttcag ttgacaacca ccaaaaaata atggataata actaggaaaa ttgaatggag
     1861 gaagccatta ggcttatata aattatggga aaatattttg gaacagatgg tgttcgtggc
     1921 gaagcaaatg ttgagctgac acctgagtta gcttttaaac tgggccgatt cggtggttat
     1981 gttttaagtc aacatgagac cgaaagacct aaagtttttg tggcgcgtga cacccgtatt
     2041 tcaggagaaa tgttggagtc agccttgatt gctggtctac tatctgttgg tattgaagtt
     2101 tataaacttg gcgttttagc gacacctggt gtctcttatt tagttagaac agaaaaagca
     2161 agtgcaggtg tgatgatttc tgcaagtcat aaccctgcgc ttgacaatgg catcaaattc
     2221 tttgggaatg atgggtttaa gttagctgat gaccaagaat tggaaattga agctctctta
     2281 gatgctccgg aggacacgct tcctcgcccg agtgcagaag gacttggaac cttggtagat
     2341 tatccagaag gacttcgtaa atatgaaaag ttcttggtaa caactggtac tgacttaagc
     2401 ggtatgacag ttgccttaga cactgctaat ggagctgctt cagtgtcagc acgtgatgtc
     2461 tttttagatt taaatgcaga aattgctgtc attggtgaaa aaccaaatgg tcttaatatt
     2521 aatgacggtg ttggttcaac gcatccagaa caacttcaag agttagttaa ggaaacagga
     2581 gcagatcttg gtttagcctt tgatggcgat agcgaccgtc tgattgccgt ggacgaaact
     2641 ggcgagattg ttgacggtga tcgtattatg tttatcattg gtaaatactt gtcagaaaaa
     2701 gggcttcttg ctcacaatac cattgtcaca acggtgatgt ctaacctcgg ttttcataaa
     2761 gctttagata aacaaggtat taataaagca attacagcag ttggagaccg ctatgtggtt
     2821 gaagaaatgc gtagctcagg atacaatctt ggtggtgaac aatcaggcca tgtcattatc
     2881 atggactaca ataccactgg agatggtcag ttgacagcta tccaattagc caaggtgatg
     2941 aaggaaactg ggaaatcact ttcggaatta gctgctgaag taaccattta ccctcaaaaa
     3001 ctggtgaata ttcgtgtgga aaacagcatg aaagaccgtg ctatggaagt tccagctatt
     3061 gcgaacatca ttgcaaaaat ggaggatgaa atggctggta atggtcgtat tctcgtgaga
     3121 ccaagtggta cagaacctct tcttcgtgtc atggcagagg cgccaacaga tgccgaagtt
     3181 gattattatg tcgatactat tgctgatgtc gtccgtacag aaatcggatg cgataactaa
     3241 cgagttgagt acaaacaaga aaccttattt ctaaggcttc tgtcaacaat tggaaagagg
     3301 cttggggcgt ttagcccaag tctcttttta taaattgcta aagtagaaga gaaaggatat
     3361 cggatgacaa aacagcaaat cgaaaaacta gcggacttac tgcctccttt tgcagccaca
     3421 ctttacttag atatgattcc tgaatttcag gaaggttatg cttcatatgt ttatcaaaca
     3481 ccaaaacaag atattcaaaa gcgccgcatc aagcagttaa ctaaaaattt tgaacgatta
     3541 ctagataccc catcttttta tgtgatgcct gtgacaagaa aaacagcaga gcaattgcta
     3601 gcagataaag gcctagctta tcatttgtta tcgacagttg cagctaatca agtgtttgcg
     3661 gctgctgtta acgaggatca gacgcttttt caagtgcttc gaaatggagc aagagtagcc
     3721 tttgtgattc ttcaagaaaa gggggaattt ctagaaatga ctttagggat tcttgaaaca
     3781 gtgacagaaa acgaggttcc agcggctatt gtggaggctg tccagacata tcttctggca
     3841 gatatgcaag agaacctccc ttctttaagc attatggtca gtcaagaatc aagcaagctt
     3901 ttttaccaag aacaaggatt tgttttgcta gctaagggtt gcaagccagg aaacgaaaat
     3961 gattctgacg tttgtcagct cgtaaaatat ttttccaggc catgaagaat caagggaaat
     4021 ggcttattca tagaaacatt ttatcatcaa agtggtataa taagaccatg tcaaaaaaac
     4081 ctacttcagc ctatgtgcac attccttttt gcacacaaat ttgttattat tgcgattttt
     4141 caaaagtctt tatccaaaat caacctgttg atgcttattt gaaagccttg attcaagaat
     4201 ttgactctta tggcattcga gacctaaaaa ccctttatat tggaggaggt acgcctacag
     4261 ctatcactgc taaacagttg gagtatctcc taaatcacct tgaacgaaac ctcaatttag
     4321 atgatcttga agaattcaca attgaggcaa atcctggtga tttgacgcca gaaaaaattg
     4381 cggtgcttca gaggtcagct gtcaatcgga tttccttggg ggtgcagact tttaataaca
     4441 agcaattaaa gcagattggc cgtagccata atgaagaaca aatttatagc accattgcca
     4501 atttaaaaac tgcaggcttt cacaatattt ccattgattt aatttatgcc ttgccaggtc
     4561 aaaccttaga tcaagtcaag gaaaatgttg ctaaagctct ggcacttgat attcctcacc
     4621 ttagtcttta tagccttatt ttggaacatc acactgtttt tatgaacaaa atgcgacgtg
     4681 gtaaacttaa cttaccaacg gaagacttgg aagcggaaat gtttgagtac attatttcag
     4741 aaatggaagc taatggtttt gagcattatg aaatttccaa ttttaccaaa ccaggttttg
     4801 aaagtcgcca taacttgatg tattgggata atgtggagta ttttggatgt ggtgcagggg
     4861 cttcaggtta ccttaatggc attcgttatc agaatcgggt gcccatccag cattatctta
     4921 aagcggttga agcagggaat gctagactaa atgaagaagt gctgcgcaaa gaggaaatga
     4981 tggaagagga actctttttg ggcttacgta aaaagacagg agtctccatc cagcggttcc
     5041 aagaaaaatt tggtgtttct tttgaggagc gttatggaaa tattgttagg gagcttcaaa
     5101 accaaggact gcttgtcaaa gatgacgctt ttgtcagaat gactaaaaag ggtcttttct
     5161 taggtgattc tgttgctgag cggtttatat tagattagga gagtattatg ggattaagtt
     5221 atcaggagga gttgacactt ccttttgaat tatgtgatgt caaatcagat ataaaattgc
     5281 cccttttatt agactattgt ttgatggttt ctggtagaca gtctgcgcaa ttaggacgaa
     5341 gcaacaacaa ccttttagtc gattacaagc ttgtttggat tgtaacggat tatgagatca
     5401 ctattcatcg cttgccacat tttcaagaaa ccatcaccat tgaaacaaaa gccctctcct
     5461 acaataaatt tttttgttat cgccaatttt atatttatga tcaagagggg ggtcttttag
     5521 tggatatctt agcctatttt gctttgttaa acccagatac gcgaaaagtg gcaactattc
     5581 cagaagattt agtagcgcct tttaagactg attttgttaa aaagccacac cgtgttccta
     5641 aaatgcctct tttagaacaa tcaattgatc gtgattatta tgtgcgttat tttgatattg
     5701 atatgaatgg tcatgtcaac aacagtaaat atttagattg gatgtatgat gtgttggggt
     5761 gtgagttttt aaaaacgcat cagcctctta agatgacttt gaaatatgtt aaagaagtct
     5821 caccaggcgg tcaaattact tccagttacc atttggatca attaacttct taccatcaaa
     5881 tcacctcaga tgggcagctg aatgcccaag ccatgattga atggcgagcg attaaacaaa
     5941 cagaaagcga gacagactag tgccttataa gggttattta attgatttag acggaacgat
     6001 ttaccaaggg aaaaaccgta ttccagcagg ggagcggttt atcaaacgcc tacaagaaag
     6061 aggcattccc tatttattag tgactaataa taccacaaga acacccgaaa tggtgcaaag
     6121 catgctggct aatcagtttc atgttgagac aggcattgag accatttata cggctaccat
     6181 ggcaaccgtc gattacatga atgacatgaa ccgtggcaag acagcttacg ttattggaga
     6241 aacagggctc aaatcagcta ttgcagcagc aggttacgta gaagaacttg agaatcccgc
     6301 ttacgtggtg gttggtttag acagtcaggt gacctatgag atgttggcta tagccacctt
     6361 ggctattcaa aaaggtgccc tctttattgg gacaaatcct gatttaaaca ttccaaccga
     6421 acgtggtttg atgccaggag caggtgcatt gaatgcctta ctagaagctg ccacacgtgt
     6481 aaaacctgtt tttattggca agcccaacgc tatcattatg aataaatcac tagaggtatt
     6541 gggcattcag cgctctgagg ctgtcatggt tggcgataat tacttgactg atatcatggc
     6601 aggtattcaa aatgatattg caacgatcct cgtgacaaca ggctttacca gacctgaaga
     6661 ggtaccaacc ttaccaattc agccagatca tgtcctatct agccttgatg agtggaggtt
     6721 gtgatggtag aaaacactaa attactatgt agctgggtat ggttattagc gttagctatt
     6781 ctcataacga tctattcaac gtggctgtgg tatcctcttg aggtggacca tctaaagtta
     6841 gaacaagttg tttttatgag caaggatgct atcttgcata actataacgg actactaaat
     6901 tacctcacca atccttttgt gacaaggttg gaatttgcta attttcactc atcagcagac
     6961 ggactcaagc attttgcaga tgtgaagtgg ctttttcacc taactcaagt cgtttttctt
     7021 ggcctccttt acccaaccct taagactttc acacaaaggt taaaaaccaa acgtttctgg
     7081 ctccttcaaa aacctttaat actagcagct ttgtttcctc ttatgattgg tctaatggct
     7141 agtttcattg ggtttgaaca tttttttact ctttttcatc aggtgctttt tgtaggagat
     7201 agcagctggc tctttgatcc cttgaaagat cctgtgattt ggatccttcc agaagtcttt
     7261 ttcttgcact gtttcctttt ctttatgata gtttacgaaa tcatcctgtg gagcttagta
     7321 ggtttagcaa gatggcagcg cctaaatgta gaatgataaa acaagcgttt tgaaagcgct
     7381 tgttttttag tttgcagtca gagtagaata gaagtatcaa aaaaaagcac cgactcggtg
     7441 ccactttttc aagttgataa cggactagcc ttattttaac t
//
LOCUS       DATXIG010000064         7305 bp    DNA     linear   BCT 28-DEC-2023
DEFINITION  TPA_asm: Streptococcus pyogenes strain MGAS11113
            SAMN08555799-rid19682583.denovo.064, whole genome shotgun sequence.
ACCESSION   DATXIG010000064 DATXIG010000000
VERSION     DATXIG010000064.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN08555799
            Sequence Read Archive: SRR6740495
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pyogenes
  ORGANISM  Streptococcus pyogenes
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 7305)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7305)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-OCT-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-OCT-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001918058.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001918058.2
            Genome Coverage       :: 368x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/12/2023 11:23:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 1,806
            CDSs (total)                      :: 1,769
            Genes (coding)                    :: 1,722
            CDSs (with protein)               :: 1,722
            Genes (RNA)                       :: 37
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1, 1 (5S, 23S)
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 47
            CDSs (without protein)            :: 47
            Pseudo Genes (ambiguous residues) :: 0 of 47
            Pseudo Genes (frameshifted)       :: 30 of 47
            Pseudo Genes (incomplete)         :: 20 of 47
            Pseudo Genes (internal stop)      :: 9 of 47
            Pseudo Genes (multiple problems)  :: 11 of 47
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7305
                     /organism="Streptococcus pyogenes"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN08555799-rid19682583.denovo.064"
                     /strain="MGAS11113"
                     /serotype="Emm28"
                     /isolation_source="normally sterile site"
                     /host="Homo sapiens"
                     /db_xref="taxon:1314"
                     /country="Finland"
                     /collection_date="2002"
                     /collected_by="National Public Health Laboratory"
     gene            583..672
                     /locus_tag="VC122_001481"
     CDS             583..672
                     /locus_tag="VC122_001481"
                     /inference="COORDINATES: protein motif:HMM:NF024729.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS3 family transposase"
                     /protein_id="HEP5641398.1"
                     /translation="MIIDYCNNKRIKIKRKGLSPLQYKTKSFT"
     gene            complement(710..1633)
                     /locus_tag="VC122_001482"
     CDS             complement(710..1633)
                     /locus_tag="VC122_001482"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003046685.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEP5641399.1"
                     /translation="MSEKLVEVKDLEISFGEGKKKFVAVKNANFFIKKGETFSLVGES
                     GSGKTTIGRAIIGLNDTSSGQILYDGKVINGRKSKSEANELIRKIQMIFQDPAASLNE
                     RATVDYIISEGLYNFNLFKTEEERKEKIKNMMAEVGLLSEHLTRYPHEFSGGQRQRIG
                     IARALVMNPEFVIADEPISALDVSVRAQVLNLLKRMQAEKGLTYLFIAHDLSVVRFIS
                     DRIAVIHKGVIVEVAETEELFNNPIHPYTQSLLSAVPIPDPILERQKELVVYHPDQHD
                     YTLDKPSMVEIKPNHFVWANQAEIEKYQKEL"
     gene            complement(1626..2696)
                     /locus_tag="VC122_001483"
     CDS             complement(1626..2696)
                     /locus_tag="VC122_001483"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003049688.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEP5641400.1"
                     /translation="MTKENNVILTAKDVVVEFDVRDRVLTAIRNVSLELVEGEVLAFV
                     GESGSGKSVLTKTFTGMLESNGRIANGSIVYRGQELTDLKTNKEWAKIRGSKIATIFQ
                     DPMTSLSPIKTIGSQITEVIIKHQKVSHAKAKEMALDYMNKVGIPNAKKRFEDYPFEY
                     SGGMRQRIVIAIALACRPDILICDEPTTALDVTIQAQIVELLKSLQREYHFTIIFITH
                     DLGVVASIADKVAVMYAGEIVEFGTVEEIFYDPRHPYTWSLLSSLPQLADESGELYAI
                     PGTPPSLYSPIIGDAFALRSEYAMVLDFEKAPPAINVSETHWAKTWLLHPEAPKVQKP
                     EVIQDLHQKILRKMSQQEEGNV"
     gene            complement(2705..3631)
                     /locus_tag="VC122_001484"
     CDS             complement(2705..3631)
                     /locus_tag="VC122_001484"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009569326.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEP5641401.1"
                     /translation="MESIDKSKFRFVERDSEASEVIDTPAYSYWKSVFRQFFSKKSTV
                     FMLVILVTVLMMSFIYPMFANYDFNDVSNINDFSKRYIWPNAEYWFGTDKNGQSLFDG
                     VWYGARNSILISVIATLINITIGVVLGAIWGVSKAFDKVMIEIYNIISNIPSMLIIIV
                     LTYSLGAGFWNLILAFCITGWIGVAYSIRVQILRYRDLEYNLASQTLGTPMYKIAVKN
                     LLPQLVSVIMTMLSQMLPVYVSSEAFLSFFGIGLPTTTPSLGRLIANYSSNLTTNAYL
                     FWIPLVTLILVSLPLYIVGQNLADASDPRSHR"
     gene            complement(3631..5133)
                     /locus_tag="VC122_001485"
     CDS             complement(3631..5133)
                     /locus_tag="VC122_001485"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002991105.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEP5641402.1"
                     /translation="MKKYILNRIMRSLVSVVLVTALTYTIVYTLVPTSLIFKQDPNYN
                     KMTTTPDKKVAYENLTFQRMGYVNYFSSKELKDNASKVDSSVTTEATSANKAIYEKYI
                     DSLGNGWQLKRFPTSKQFYAIRNIPIYERVWNFFSNLVVIDHPWKIQDKDNPKLARYI
                     RLEKDKSVGWSLVGSGTKHKYLLYTNGKFPYLHQNFVTLNLGTSYPTYSNIPVLQVIS
                     QGQGRTALQDVTFPSGVTKKSSVDIYSRSYKNPKSLDDITKVNYGKGDSYTKTINNYV
                     DPSMIHNSFVIGFFGVMFSYIVGLPLGLFMARFKNTYFDRFSTATMTFMLALPSIAVI
                     YVVRFLGGMAGLPDSFPMLGASDPKSYILPALILGILNIPTTVIWFRRYLVDLQASDW
                     VRFARSKGLSESEIYRGHLFKNAMVPIVSGVPASIILAIGGATLTETVFAFPGMGKML
                     IDSIKSANNSMIVGLTFIFTVLSIVSLLLGDIVMTLVDPRIKLSTKKGGK"
     gene            complement(5198..7168)
                     /locus_tag="VC122_001486"
     CDS             complement(5198..7168)
                     /locus_tag="VC122_001486"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011285389.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide ABC transporter substrate-binding
                     protein"
                     /protein_id="HEP5641403.1"
                     /translation="MKKSKWLAAVSVAILSVSALAACGNKNASGGSEATKTYKYVFVN
                     DPKSLDYILTNGGGTTDVITQMVDGLLENDEYGNLVPSLAKDWKVSKDGLTYTYTLRD
                     GVSWYTADGEEYAPVTAEDFVTGLKHAVDDKSDALYVVEDSIKNLKAYQNGEVDFKEV
                     GVKALDDKTVQYTLNKPESYWNSKTTYSVLFPVNAKFLKSKGKDFGTTDPSSILVNGA
                     YFLSAFTSKSSMEFHKNENYWDAKNVGIESVKLTYSDGSDPGSFYKNFDKGEFSVARL
                     YPNDPTYKSAKKNYADNITYGMLTGDIRHLTWNLNRTSFKNTKKDPAQQDAGKKALNN
                     KDFRQAIQFAFDRASFQAQTAGQDAKTKALRNMLVPPTFVTVGESDFGSEVEKEMAKL
                     GDEWKDVNLADAQDGFYNPEKAKVEFAKAKEALTAEGVTFPVQLDYPVDQANAATVQE
                     AQSFKQSVEASLGKENVIVNVLETETSTHEAQGFYAETPEQQDYDIISSWWGPDYQDP
                     RTYLDIMSPVGGGSVIQKLGIKAGQNKDVVAAAGLDTYQTLLDEAAAITDDNDARYKA
                     YAKAQAYLTDSAVDIPVVALGGTPRVTKAVPFSGGFSWAGSKGPLAYKGMKLQDKPVT
                     AKQYENAKEKWMKAKAKSNAKYAEKLADHVEK"
BASE COUNT         2284 a         1409 c         1352 g         2260 t
ORIGIN      
        1 ttttttgtac ttttttattt gcccgcgttg taaaatgaag tgcaacaaaa aaagagaaaa
       61 agccttgaac tacaccccaa acgttagaca aaaaaatcta acgttcgggg tattttttat
      121 gaaattaagt gatgaaagta cgaaatagac atcgttaaga aagaaaaaaa tacctactat
      181 ccaccagaga taaagcaaga aataatggat aacgttttga tagaaggccc gccacaatac
      241 caagtatcac ttgtttatgg cttgttacat gcaggaactc tcccaagttg aatagtacaa
      301 tataagaaaa acagttatac tgttgagaaa acaagagcaa gaccatcaca aacaggacgt
      361 aaaccaaaag aaacctgaag aaatgactaa attagagcgg cttcaagaag aaaatgaata
      421 tctcagaact gaggttgctt acttaaaaaa accgaggaac tccgtttgaa agaagcatct
      481 ctgtaaagaa gaagacaaaa aaaccattat aaaaaatgga caaagacgag ttctgattag
      541 attttctttt aagaacggct gtattgactc gttctaccta ccattatcat cgattattgc
      601 aacaataaac gtatcaaaat aaaacgaaaa ggacttagtc ctctacaata caaaactaaa
      661 tcctttactt aaaatagagt agccaacttt tggggtcagt gcatctcttt tacaattctt
      721 tttgatattt ttcaatttct gcttggtttg cccaaacaaa gtgatttggt ttgatttcaa
      781 ccattgatgg cttatctaat gtataatcat gttggtctgg atgatagaca acaagttctt
      841 tttgacgctc taaaattgga tctgggatag gcacggctga taacaaagat tgggtgtagg
      901 gatgaattgg gttattaaac agttcttctg tttctgcaac ttctacaata acccctttat
      961 ggataaccgc aatacgatct gaaataaagc gaacgactga aagatcatgg gcaatgaaga
     1021 gataagtcaa acctttttcg gcttgcatcc gtttgagaag gtttaaaacc tgtgcgcgaa
     1081 cggaaacgtc caaagctgaa atcggttcat cagcaataac gaattcaggg ttcattacta
     1141 gggctctagc gataccgatc cgctgacgtt gacctcctga aaattcatga gggtagcgcg
     1201 tcaaatgctc tgatagcaaa ccaacttcgg ccatcatgtt cttaattttt tctttacgtt
     1261 cttcctctgt tttaaacaga ttaaagttat aaagaccttc tgaaatgatg tagtcaacgg
     1321 ttgcccgttc attcaaacta gcagcgggat cttggaaaat catttgaatt ttacgaatga
     1381 gctcattggc ttctgatttt gattttctgc cattaattac tttcccatcg tataaaattt
     1441 gacctgagct agtatcgttc aaaccaataa tagcacgacc aattgttgtt ttaccactcc
     1501 cagattctcc aactaaagaa aaggtttctc cttttttaat aaagaaatta gcatttttaa
     1561 ctgcaacaaa ttttttcttt ccttctccga aggaaatttc taggtctttt acttcgacta
     1621 atttctcaga catttccttc ctcctgttgt gacattttcc ttaagatttt ttgatgcaaa
     1681 tcttgaatga cttctggttt ttgaactttt ggagcctctg ggtgtaaaag ccatgttttg
     1741 gcccaatgag tctcagatac gttaatcgcc ggaggtgctt tttcaaagtc taaaaccata
     1801 gcatattctg agcgaagtgc aaaggcatct ccgataattg gtgaataaag tgatggaggc
     1861 gttcctggaa tagcgtaaag ttcaccagat tcatctgcca actgcggtaa gctagacagc
     1921 aaactccatg tataggggtg ccttggatca tagaaaatct cttcgactgt tccaaattca
     1981 acaatttctc ctgcatacat gacagccact ttatctgcaa tgcttgccac aacacctaaa
     2041 tcgtgcgtaa taaagataat ggtgaaatga tattctcgtt gaagcgactt caataactca
     2101 acgatttgag cttgaatagt cacatcaagg gctgttgttg gctcatcaca gataagaata
     2161 tctgggcgac aagctaaagc aatagcgata acaatacgtt gacgcattcc tcctgaatac
     2221 tcaaatgggt aatcttcaaa gcgttttttg gcatttggga tacccacttt attcatgtaa
     2281 tcaagggcca tttctttagc tttggcatga cttacttttt ggtgcttaat aatcacttct
     2341 gtgatttggc taccaatagt tttaatggga ctaagactgg tcattgggtc ttggaaaatc
     2401 gttgcgattt ttgagccgcg aatctttgcc cactctttat ttgtttttaa atctgtcaat
     2461 tcttgcccac gatagacaat tgaaccatta gcaatgcgtc cattagactc caacatccct
     2521 gtaaaggtct ttgttaaaac agatttacct gagcctgatt cccctacaaa agcaaggact
     2581 tctccttcaa caagttccag tgagacgtta cggatagctg ttaaaacacg atcacgcaca
     2641 tcaaattcta ccaccacatc tttggcagtt aagattacat tattttcttt tgtcatttta
     2701 actcctatct atgtgaacgt gggtcactgg catcagccaa gttttgtccg acaatgtata
     2761 gtggtaacga tactaaaatc aatgttacta agggaatcca aaagaggtag gcatttgttg
     2821 ttaagttgct tgaataatta gcaatcaaac gtcctaaact tggagtggtg gttggtaaac
     2881 caatcccaaa gaaggataag aaggcctcag aagatacata aactggtagc atttgtgaca
     2941 acatagtcat gataactgaa accaattgag gcaggaggtt cttaacagca atcttgtaca
     3001 ttggtgttcc caaagtttga ctagcaaggt tgtattctaa atcacggtaa cgcaagattt
     3061 gaacacggat ggagtaggcg acaccaatcc atccagtgat acagaaagct agaatcaaat
     3121 tccaaaatcc tgcacctaat gaataggtca aaacaatgat aataagcata gaagggatat
     3181 ttgagataat gttataaatt tcaatcataa ctttatcaaa tgctttagaa actccccata
     3241 tggctcctaa cactacccca atggtgatat taattagtgt cgctataact gagattaaaa
     3301 tagaattacg tgccccatac caaacaccat caaacagaga ttgcccattt ttgtcggttc
     3361 caaaccagta ctctgcattt ggccaaatat aacgctttga aaagtcattg atattactaa
     3421 cgtcattaaa gtcgtagttg gcaaacattg gataaataaa gctcatcatc aagactgtca
     3481 ctaaaatcac gagcataaag actgtagatt ttttagaaaa aaactgacga aacactgatt
     3541 tccagtaaga ataagcaggg gtatcaatca cttcggaggc ttcactatcg cgctcaacaa
     3601 atcgaaattt agatttatca atcgattcca ttacttacct cctttttttg ttgacaattt
     3661 aatacgtgga tcgacaaggg tcatgacaat atctcctagt aaaagtgaaa caattgacaa
     3721 gacagtgaag ataaaggtta atccaacaat catagaattg ttagcagatt taatagaatc
     3781 aattaacatt ttacccatac ccgggaaggc aaagacagtc tctgttaagg ttgcaccacc
     3841 aatagcaagg ataatacttg caggaacccc tgacacaatt gggaccatgg catttttgaa
     3901 gagatgccct ctgtaaattt cagattctga taatcctttt gaacgcgcaa aacgtaccca
     3961 atcactggcc tgaaggtcaa caagatagcg acggaaccag ataaccgtag ttggaatatt
     4021 taaaattcct aaaataaggg ctggtaaaat ataagattta ggatcagatg cccctagcat
     4081 tgggaaacta tctggtaaac cagccatgcc acctaggaag cgaacaacat agataacagc
     4141 aatacttggt aaagcaagca tgaaggtcat ggtcgctgtt gagaaacggt caaaataggt
     4201 atttttaaac cgagccataa ataaaccaag aggcaagcca acgatatagg aaaacataac
     4261 accaaaaaaa ccaattacaa aagaattgtg aatcattgat gggtcgacat aattattgat
     4321 ggttttagtg taggaatctc ccttaccata gtttaccttg gtgatatcat ccaatgattt
     4381 tggatttttg tagctacggc tatagatatc cactgacgat ttcttagtga cacctgatgg
     4441 gaaggtcaca tcttgaagag ctgttcgtcc ttgcccttgt gaaataacct gaagaacagg
     4501 aatattgctg tatgttgggt aagatgttcc taagtttagg gtaacaaagt tttggtgaag
     4561 ataaggaaat tttccgttag tgtagaggag atatttatgt tttgtccccg aaccaacaag
     4621 tgaccagcca actgatttat ctttttctag acgaatatac ctagctaatt ttggattgtc
     4681 tttatcctga atcttccaag ggtgatcaat aacaactaga tttgagaaaa agttccaaac
     4741 acgttcgtaa ataggaatat tgcgaattgc ataaaactgt ttgctagtgg ggaagcgttt
     4801 tagctgccag ccattgccaa gagaatcaat atacttctca tagatagctt tgttagcact
     4861 agttgcttct gtcgttacac tactatctac tttagaagca ttatctttta attctttgct
     4921 tgaaaaataa ttgacataac ccatacgttg aaaagttaga ttttcatacg ctactttttt
     4981 atctggtgtt gttgtcattt tattataatt agggtcttgt ttaaagataa ggctggtagg
     5041 tactagagta tatacgatgg tgtatgttaa agccgttacc aaaactaccg acactaacga
     5101 tcgcatgata cggtttaaaa tgtatttttt catagttatc ctcaatagtt caaaagaact
     5161 tcctcacata aacgagaaag ttcttttgaa catcgtttta tttttcaacg tgatcagcta
     5221 acttctcagc atattttgca tttgacttag cctttgcttt catccatttt tcttttgcat
     5281 tttcgtattg ttttgctgtg acaggtttgt cttgaagttt cattccttta tatgctagag
     5341 gacctttaga ccctgcccaa gagaagcccc cgctaaatgg aacggcttta gtaactcgtg
     5401 gagtgccacc caatgccaca actggaatat ctacggcact atctgtaagg taggcttgtg
     5461 cttttgcgta agctttatag cgcgcatcgt tgtcgtctgt aattgctgct gcttcatcaa
     5521 ggagagtttg gtaagtatca aggcctgcag ctgccacaac atccttattt tgacctgctt
     5581 tgattccaag tttttggata acagatccac cacctactgg actcatgatg tcaaggtagg
     5641 tccgtggatc ttggtagtct ggtccccacc atgatgaaat gatatcgtag tcttgttgtt
     5701 ctggggtctc agcatagaag ccttgggctt cgtgagttga tgtttctgtt tcaagaacat
     5761 tgacaatgac attttcttta ccaagagatg cttcaacaga ttgtttgaaa gactgggctt
     5821 cctgaacagt tgctgcgttt gcctggtcaa cagggtaatc taattgaact gggaaggtta
     5881 caccttcagc tgtcaaagct tctttggctt ttgcaaattc aacttttgct ttttcaggat
     5941 tatagaaacc atcttgagca tcagctaagt taacgtcttt ccattcatca ccaagttttg
     6001 ccatttcctt ttcaacttct gaaccaaaat cactttctcc aacggttaca aaagttggtg
     6061 gcacaagcat gttacgtaag gcttttgttt tggcatcttg acctgcagtt tgtgcttgga
     6121 atgacgctcg gtcaaaagca aactgaatag cttgacgaaa atccttgttg ttaagagctt
     6181 tcttaccggc atcttgttgt gcagggtctt tcttagtgtt tttgaaagaa gtacggttca
     6241 aattccatgt taaatgacgg atatctccag tcaacattcc gtaagtaata ttatcagcat
     6301 agtttttctt agctgatttg taggtagggt catttgggta aagtcgtgca acgctgaact
     6361 cacccttgtc aaagttcttg tagaacgaac ctgggtctga accatctgag taagtcaatt
     6421 taacagattc tatcccaaca ttcttagcat cccagtagtt ttcattttta tggaattcca
     6481 tagatgattt tgaggtgaag gcgctcaaga agtaagcacc attaacaagg attgatgatg
     6541 gatcggttgt accaaaatct ttaccttttg acttcaaaaa tttcgcatta actgggaaaa
     6601 gcacactata agttgttttt gaattccagt agctttcagg cttgttcaaa gtatactgaa
     6661 cagttttatc gtcaagggct ttgacaccaa cttctttaaa atctacttca ccattttggt
     6721 aagcctttaa gttttttatt gaatcttcaa caacgtaaag agcatctgat ttatcgtcaa
     6781 ccgcgtgctt caaaccagtc acaaaatctt ctgctgttac tggggcatat tcttcaccat
     6841 cagccgtata ccaagagaca ccatcgcgaa gagtataagt ataagtcaga ccgtcttttg
     6901 aaaccttcca atctttagca agtgatggta ctaaattacc atactcatcg ttttccaaaa
     6961 gaccatcaac catttgtgtt atcacatcag tcgttccacc gccattagtc aaaatataat
     7021 ccaatgattt tggatcgtta acaaaaacgt acttgtaggt ttttgtagct tctgagccac
     7081 ctgaagcatt tttattacca caagctgcca aagcggatac tgacaagatc gcaacactta
     7141 cagctgccaa ccatttactt ttcttcataa aagtcacccc taaactttat ttttcatttt
     7201 ttggcaattg ctaaatgaaa ttggttaatt gaagacatta tatcataaaa ctttcttaaa
     7261 gaaaataatg aaatgaaaaa ttatctgaat atttcgtaga atttt
//
LOCUS       DATXIG010000065         7102 bp    DNA     linear   BCT 28-DEC-2023
DEFINITION  TPA_asm: Streptococcus pyogenes strain MGAS11113
            SAMN08555799-rid19682583.denovo.065, whole genome shotgun sequence.
ACCESSION   DATXIG010000065 DATXIG010000000
VERSION     DATXIG010000065.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN08555799
            Sequence Read Archive: SRR6740495
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pyogenes
  ORGANISM  Streptococcus pyogenes
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 7102)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7102)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-OCT-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-OCT-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001918058.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001918058.2
            Genome Coverage       :: 368x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/12/2023 11:23:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 1,806
            CDSs (total)                      :: 1,769
            Genes (coding)                    :: 1,722
            CDSs (with protein)               :: 1,722
            Genes (RNA)                       :: 37
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1, 1 (5S, 23S)
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 47
            CDSs (without protein)            :: 47
            Pseudo Genes (ambiguous residues) :: 0 of 47
            Pseudo Genes (frameshifted)       :: 30 of 47
            Pseudo Genes (incomplete)         :: 20 of 47
            Pseudo Genes (internal stop)      :: 9 of 47
            Pseudo Genes (multiple problems)  :: 11 of 47
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7102
                     /organism="Streptococcus pyogenes"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN08555799-rid19682583.denovo.065"
                     /strain="MGAS11113"
                     /serotype="Emm28"
                     /isolation_source="normally sterile site"
                     /host="Homo sapiens"
                     /db_xref="taxon:1314"
                     /country="Finland"
                     /collection_date="2002"
                     /collected_by="National Public Health Laboratory"
     gene            137..1372
                     /gene="arcA"
                     /locus_tag="VC122_001487"
     CDS             137..1372
                     /gene="arcA"
                     /locus_tag="VC122_001487"
                     /EC_number="3.5.3.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012560827.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arginine deiminase"
                     /protein_id="HEP5641404.1"
                     /translation="MTAQTPIHVYSEIGKLKKVLLHRPGKEIENLMPDYLERLLFDDI
                     PFLEDAQKEHDAFAQALRDEGIEVLYLETLAAESLVTPEIREAFIDEYLSEANIRGRA
                     TKKAIRELLMAIEDNQELIEKTMAGVQKSELPEIPASEKGLTDLVESNYPFAIDPMPN
                     LYFTRDPFATIGTGVSLNHMFSETRNRETLYGKYIFTHHPIYGGGKVPMVYDRNETTR
                     IEGGDELVLSKDVLAVGISQRTDAASIEKLLVNIFKQNLGFKKVLAFEFANNRKFMHL
                     DTVFTMVDYDKFTIHPEIEGDLRVYSVTYDNEELHIVEEKGDLAELLAANLGVEKVDL
                     IRCGGDNLVAAGREQWNDGSNTLTIAPGVVVVYNRNTITNAILESKGLKLIKIHGSEL
                     VRGRGGPRCMSMPFEREDI"
     gene            1472..1900
                     /locus_tag="VC122_001488"
     CDS             1472..1900
                     /locus_tag="VC122_001488"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014407703.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HEP5641405.1"
                     /translation="MTDKFDANDETRTVYAVVYDNDQPVSTGQFLAETKIEARLTRIV
                     TLADYCGCGYGAKVTEALETYTRREGFYQLTIHSELTAQTFYENLGYQSYGPKCLEDG
                     EYCQSLAKTILKWEKNMDIAMLIAIVGGLLGCYLYLTKNN"
     gene            1940..2953
                     /gene="argF"
                     /locus_tag="VC122_001489"
     CDS             1940..2953
                     /gene="argF"
                     /locus_tag="VC122_001489"
                     /EC_number="2.1.3.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000195397.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ornithine carbamoyltransferase"
                     /protein_id="HEP5641406.1"
                     /translation="MTQVFQGRSFLAEKDFTRAELEYLIDFSAHLKDLKKRGVPHHYL
                     EGKNIALLFEKTSTRTRAAFTTAAIDLGAHPEYLGANDIQLGKKESTEDTAKVLGRMF
                     DGIEFRGFSQRMVEELAEFSGVPVWNGLTDEWHPTQMLADYLTVKENFGKLEGLTLVY
                     CGDGRNNVANSLLVTGAILGVNVHIFSPKELFPEEEIVTLAEGYAKESGARILITEDA
                     DEAVKGADVLYTDVWVSMGEEDKFKERVELLQPYQVNMNLVQKAGNDKLIFLHCLPAF
                     HDTNTVYGKDVAEKFGVKEMEVTDEVFRSKYARHFDQAENRMHTIKAVMAATLGNLFI
                     PKV"
     gene            3123..4616
                     /locus_tag="VC122_001490"
     CDS             3123..4616
                     /locus_tag="VC122_001490"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002983810.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YfcC family protein"
                     /protein_id="HEP5641407.1"
                     /translation="MTEEKKRGFRIPSSYTVLFIIIAIMAVLTWFIPAGAYETAKGGG
                     VISGTYKTVASNPQGFFDILMAPVRGMLGVEGTDGAIQVSFFILMVGGFLGVVNKTGA
                     LDTGIASVVRKNKGREKMLIAILIPLFALGGTTYGMGEETMAFYPLLIPVMIAVGFDS
                     IVAVAIILIGSQIGCLASTINPFATGVAADAAGVSIADGMIWRVIQWVILVGMSIWFV
                     YNYASKIEEDPSKSLVADKEEEHKELFQLQNSGEDLNKRQRNVLTIFTLTFVIMILSL
                     IPWEDFGIKFFTNINTWLTTMPILGGVIGKTMGAFGTWYFPEITMLFIMMGVLVAIVY
                     RMSEEDFFSSFLTGAGEFLGVAMICAIARGIQVIMNGGMITATILHLGETSLSGLSSQ
                     VFVILAYIFYLPMSFLIPSTSGLAGATMGIMAPLGQFSNVPAHLVITAFQSASGILNM
                     ISPTSAIVMGALALGRVDLGTWWKFIGKFIVMVMLVSVLLLVVATFF"
     gene            4633..5964
                     /locus_tag="VC122_001491"
     CDS             4633..5964
                     /locus_tag="VC122_001491"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011888730.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dipeptidase"
                     /protein_id="HEP5641408.1"
                     /translation="MESYITPKHQEACVAAIKQIVSYPSVCHEGENGTPFGQAIQDVL
                     EATLDLCQGLGFHTYIDPEGYYGYAELGDQKEVLAILCHLDVVPEGDRQLWKTDPFDC
                     VEADGCLFGRGTQDDKGPTMMALFATKALIDAGVTFNKRIRFIFGTDEETLWRCMNRY
                     NDVEEQATFGFAPDSSFPLTYAEKGLLQAKLVGKGSDKLSLEIGQAYNVVPARASYKG
                     NKLEALQKELDKLGFEYVVKEGELTVYGLAQHTKDAPDGINAMIRLAKALVVLEPNKT
                     LDFLANVIDEDGRALNIFGRIEDEPSGKLSFNAAGLTLTKDKAEIRLDIRIPVLADKE
                     KLVQQLSQKAQEYGLTYEEFDYLAPLYVPLDSELVTTLLSVYRKKTGDQSPAQSSGGA
                     TFARTMKNCVAFGALFPDAVQTEHQENEHIVLADAYRAMDIYAEAIYRLTR"
     gene            5984..6934
                     /gene="arcC"
                     /locus_tag="VC122_001492"
     CDS             5984..6934
                     /gene="arcC"
                     /locus_tag="VC122_001492"
                     /EC_number="2.7.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012679157.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbamate kinase"
                     /protein_id="HEP5641409.1"
                     /translation="MTKQKIVVALGGNAILSTDASAKAQQEALISTSKSLVKLIKEGH
                     EVIVTHGNGPQVGNLLLQQAAADSEKNPAMPLDTCVAMTEGSIGFWLVNALDNELQAQ
                     GIQKEVAAVVTQVIVDAKDPAFENPTKPIGPFLTEEDAKKQMAESGASFKEDAGRGWR
                     KVVPSPKPVGIKEANVIRSLVDSGVVVVSAGGGGVPVVEDATSKSLTGVEAVIDKDFA
                     SQTLSELVDADLFIVLTGVDNVYINFNKPDQAKLEEVTVSQMKEYITQDQFAPGSMLP
                     KVEAAIAFVENKPNAKAIITSLENIDNVLSANAGTQIIAG"
BASE COUNT         2126 a         1350 c         1515 g         2111 t
ORIGIN      
        1 atgtaagcga ttactcaaaa tagctgtgag aaatacccta tttttatctt ttttaaggag
       61 tacactagga atataagcaa gagttaagaa tcttgacggt gaattcacaa aaaccaatta
      121 gaaagaggta attcttatga ctgctcaaac accaattcat gtttattctg aaattggaaa
      181 attaaaaaaa gtgttgctcc acagacccgg taaagaaatt gaaaatctta tgccagacta
      241 cttggaacgc ttgttgtttg atgatattcc attcttagag gatgcccaaa aagaacatga
      301 tgcttttgcc caagctttgc gtgacgaagg tattgaagtc ttgtaccttg agaccttagc
      361 tgcagaatct cttgtcactc cagaaattcg cgaagctttc atcgatgaat acctaagtga
      421 agctaatatc cgtggaagag ctactaaaaa agccattcgt gaattgttga tggctatcga
      481 agacaatcaa gaattgattg aaaaaaccat ggcaggagtt caaaaatctg aacttccaga
      541 gatcccagca tctgaaaaag gcttgactga cttggttgaa tccaattacc catttgcgat
      601 cgacccaatg ccaaaccttt atttcacacg ggacccattc gcaactatcg gtacaggtgt
      661 ttctcttaac cacatgttct cagaaacacg taaccgtgaa accctttatg gtaaatacat
      721 tttcactcat cacccaattt atgggggtgg caaagtgcct atggtttatg accgtaatga
      781 aaccactcgc attgaaggtg gggacgaact tgttctttca aaagatgtgc ttgcggttgg
      841 tatttctcaa cgtacagatg ctgcttctat tgaaaaattg ttggttaaca tctttaaaca
      901 aaaccttggc ttcaagaaag tattggcctt tgaatttgca aataaccgta aattcatgca
      961 cttagacact gtctttacca tggttgacta tgacaaattt accattcacc cagaaattga
     1021 aggcgacctt cgtgtttact ctgtcactta cgacaatgaa gaacttcata tcgttgaaga
     1081 aaaaggtgat ttagcagaac ttcttgctgc taaccttggt gttgaaaaag ttgaccttat
     1141 ccgttgtggt ggtgacaact tagtagcagc aggtcgtgaa caatggaacg atggttctaa
     1201 cacccttact atcgcaccag gtgtggttgt ggtttataac cgtaacacca ttaccaatgc
     1261 tattcttgaa tctaaaggct tgaaattgat caagattcac ggaagtgaat tggttcgcgg
     1321 tcgtggtgga cctcgttgta tgtcaatgcc atttgaacgt gaagatattt aataagctat
     1381 ggtaaaggtg gttataggtc agaagccttt ttaaagggca gctagtgttt atcttcgctt
     1441 ctgtgtcttt gtccttcaat gaaattttgt catgacagat aaatttgatg ccaatgacga
     1501 aacaagaacg gtttatgcag tcgtttatga caatgaccag cccgtttcaa caggacaatt
     1561 tttagctgaa acgaaaatag aagcacgatt gacacgcatt gtaaccttag cagattattg
     1621 tggttgcggt tatggtgcca aagtcactga agcgctagaa acttatacca gacgagaagg
     1681 cttttaccaa ctaaccattc acagtgaact gacagcacaa accttttatg aaaacctagg
     1741 ttatcagtcc tatggtccca agtgtttaga agatggtgag tattgtcagt cccttgcaaa
     1801 aaccattctt aaatgggaga agaatatgga catagcaatg ctaattgcga ttgttggtgg
     1861 tctattaggc tgctatctct atctcacaaa aaataattaa tcaaaacaga ttacagttaa
     1921 cactcgaagg agacaataga tgacacaagt atttcaagga cgtagcttcc tagcagaaaa
     1981 agattttaca cgcgctgaat tagaatacct tattgatttt tcagcacatt tgaaagattt
     2041 gaaaaaacgt ggtgtgcctc atcactactt agaaggtaaa aacattgccc tcttgtttga
     2101 aaaaacatca actcgtacgc gtgcagcttt tacaacagca gccattgacc taggtgctca
     2161 cccagaatac ctcggtgcca atgacatcca acttggtaaa aaagaatcaa cagaagacac
     2221 tgctaaagta ttgggtcgta tgtttgatgg gattgaattc cgtggcttta gccaacgcat
     2281 ggttgaagaa ttggcagaat tctcaggtgt gccagtttgg aatggtttga ctgatgaatg
     2341 gcacccaacc caaatgcttg ctgactattt aacagtgaaa gaaaactttg gcaaacttga
     2401 aggccttacc cttgtttact gtggtgacgg gcgtaataac gttgccaatt cccttcttgt
     2461 gactggtgct atccttggtg ttaatgttca tatcttctca ccaaaagaac ttttcccaga
     2521 agaagagatt gtgacattgg ctgaaggcta tgctaaagaa agtggcgctc gtatccttat
     2581 cacagaagat gctgacgaag ctgttaaagg cgcagacgtg ctttacacag atgtttgggt
     2641 atcaatgggt gaagaagaca aattcaaaga acgtgttgaa ttgcttcaac catatcaagt
     2701 caacatgaac cttgttcaaa aagcaggaaa tgacaagctt atcttcttgc actgcttgcc
     2761 agctttccat gacacaaata ccgtttatgg taaagacgtt gctgaaaaat tcggcgtgaa
     2821 agaaatggaa gtcactgatg aagtcttccg cagcaaatac gctcgtcatt ttgaccaagc
     2881 tgaaaaccgc atgcacacta tcaaagcggt tatggcagca acccttggta acctctttat
     2941 tccaaaagtg taataaggac tggtactcct taactcattt cacatatcaa tgtgacccct
     3001 ttaatttaac aatttaacta cttaaaaacg tactttaact acatgacaag tcgttctggt
     3061 tttcgcgtcc ttgaccggaa ctagaacggc ttatttttat gaaaaggtaa gaggtaatca
     3121 ttatgacaga agaaaaaaaa cggggtttta gaattccttc ttcttacacc gttcttttta
     3181 tcattatagc catcatggca gtgttaactt ggtttattcc agctggcgcc tacgaaaccg
     3241 ctaagggcgg tggtgttatt tcaggaacct acaaaacagt agcatccaat cctcaaggat
     3301 tttttgacat cttgatggct cccgttcgag gcatgttagg tgttgaaggc actgatggtg
     3361 ctattcaagt gtctttcttt atcttaatgg ttgggggctt tttaggagtt gtcaacaaaa
     3421 caggtgctct tgacacaggg attgcgtcag tggttcgtaa aaacaaaggt agagaaaaaa
     3481 tgctgattgc tattttaatc cctttgtttg ctctaggtgg aacgacctat ggtatggggg
     3541 aagaaaccat ggccttttac ccacttttaa ttcctgttat gattgcggtt ggttttgaca
     3601 gtattgttgc tgtagccatt attttgattg gttctcaaat tgggtgtcta gcctccacta
     3661 ttaacccatt tgcaactggt gtagcagctg atgctgctgg tgtcagcatt gcagatggaa
     3721 tgatttggcg tgttatccaa tgggttattc ttgtcggcat gtctatttgg tttgtttaca
     3781 attacgctag taagattgaa gaagacccaa gtaaatcatt ggttgcagac aaggaagaag
     3841 agcataagga actcttccag ttgcaaaatt ctggagaaga cttaaacaag cgccaacgaa
     3901 acgttttgac catttttact ttgacctttg tcattatgat tcttagtttg attccgtggg
     3961 aagattttgg catcaaattc tttaccaata tcaatacttg gttaaccaca atgcctatct
     4021 tagggggagt tattgggaaa accatgggag catttggtac ttggtatttc ccagaaatca
     4081 ccatgctctt tatcatgatg ggtgtcttag ttgctattgt ttatcgtatg agtgaagaag
     4141 acttttttag ctcttttttg actggtgcag gtgaattttt aggtgttgcc atgatctgtg
     4201 ctattgcacg cggtattcaa gttatcatga atggtggtat gattacagcc actatcttac
     4261 acttaggtga aacaagtctt tctggtttat cttctcaagt ttttgtgata ttagcttata
     4321 ttttctacct cccaatgtcc ttcttgattc catcaacatc aggacttgct ggggctacaa
     4381 tggggattat ggcaccgctt ggacaattct caaatgtccc tgctcacctt gttattacag
     4441 ccttccagtc agcttctgga atcttaaaca tgatttctcc aacttcagca atcgttatgg
     4501 gagcacttgc gcttggtcgc gttgaccttg gtacttggtg gaaattcatt ggtaaattta
     4561 ttgtaatggt gatgcttgta agcgtgctat tacttgtagt tgcaacattc ttttaacaga
     4621 taggaggttc ctatggaatc ctatatcaca cctaaacatc aagaggcctg tgtagctgcc
     4681 attaaacaga tcgtttctta cccttctgtt tgtcatgaag gagaaaatgg cacacctttt
     4741 ggacaggcca ttcaagatgt tctcgaagca accttagact tatgtcaagg tcttggtttt
     4801 catacttata ttgatcctga aggttactat ggctatgctg agttaggtga ccaaaaagag
     4861 gtcttggcta ttctttgcca tttggacgtt gttccagaag gagatcgtca gttatggaag
     4921 acagatcctt ttgactgtgt tgaggcagac ggctgtttat ttggtcgagg cactcaagac
     4981 gataaagggc ctactatgat ggcgctattt gccaccaaag ccttgataga tgcaggtgtt
     5041 acctttaata aacgcattcg ttttattttc ggaacagatg aagaaacttt gtggcggtgt
     5101 atgaatcgct acaatgatgt agaagaacag gctacctttg ggtttgctcc tgattcaagt
     5161 ttccctttaa cctatgccga aaaaggccta cttcaggcaa aattagtggg gaaaggttct
     5221 gataaactgt cacttgaaat cggacaagcc tacaatgttg ttcctgcaag agcttcttat
     5281 aaaggcaata agcttgaggc tttacaaaaa gaactagata agcttggctt tgagtacgtc
     5341 gtcaaagagg gagaattaac ggtttatggt ctcgcccaac atactaaaga tgctcccgat
     5401 ggcatcaacg ctatgattag gcttgccaag gctttagtgg tccttgaacc taacaaaacc
     5461 cttgattttc ttgctaatgt cattgatgaa gatggcagag cacttaatat ttttggcaga
     5521 atcgaagatg aaccttcagg caaattaagc tttaatgccg cagggcttac cttaaccaaa
     5581 gacaaagcag aaattcgcct agatattcgg attccagttt tggcagataa ggaaaaatta
     5641 gtccaacaat taagccaaaa agcacaagaa tacggcctaa cctatgaaga atttgattat
     5701 ttggccccat tatatgtgcc attagacagt gaattggtaa caaccttgtt atctgtctat
     5761 cggaaaaaaa caggtgatca aagccctgcc caatcatcag gaggggcaac ctttgctaga
     5821 acaatgaaga attgtgttgc ctttggtgcc cttttccctg atgctgttca aacggaacac
     5881 caagaaaatg aacatattgt tttagcggat gcttaccgcg ctatggatat ttatgcggaa
     5941 gctatttacc gactaacgcg ataacaagat atgaggagta attatgacga aacaaaaaat
     6001 cgtagtcgca cttggaggaa atgctattct ttcaacagat gcgtcagcaa aagcacaaca
     6061 agaagccttg atttccacat caaaatcatt ggttaaatta attaaagaag gtcatgaagt
     6121 catcgtgacc cacggcaatg gcccacaagt tggtaatctc ttattacaac aagcagcggc
     6181 agattctgaa aagaatccgg ctatgccttt ggacacttgt gttgccatga cagaaggtag
     6241 catcggtttt tggttagtta atgcccttga taatgaattg caagcccaag gtattcaaaa
     6301 agaagtggca gctgttgtca ctcaagtgat cgtggatgcc aaagacccag cttttgaaaa
     6361 cccaacaaaa ccaattggtc catttcttac cgaagaagat gccaaaaaac aaatggcgga
     6421 gtctggtgca agtttcaaag aagatgcagg tcgtggctgg cgtaaggttg tgccatcacc
     6481 aaaaccagtt ggtattaaag aagccaatgt catcagaagt ctcgttgatt ctggcgttgt
     6541 tgtggttagt gctggtggtg gcggtgtgcc tgttgtggaa gatgcaacta gcaagtcctt
     6601 gactggtgtg gaagctgtta ttgacaagga ctttgctagc caaacccttt cagaattggt
     6661 ggatgcagac ctctttatcg ttttgacagg agttgacaat gtttacatca acttcaacaa
     6721 acctgaccaa gccaaattgg aagaagtgac tgtctctcaa atgaaagaat acatcacaca
     6781 agaccaattt gcaccaggta gtatgttacc aaaagtggaa gctgccattg cttttgttga
     6841 aaacaaacca aatgctaaag ccattatcac atcgctagaa aatattgaca atgtactatc
     6901 agcaaacgct ggaacacaaa ttatcgcagg gtaatcaaga ggatttttag ttctgtttaa
     6961 aagactcatt taatggtatt ttctgaagag tacgactgac ataaagagtt gatactgttt
     7021 ttaaaggaaa agagtgtgcc ataataaaaa acaatagtag tattagaaaa ggctaatttt
     7081 attgagaatt agccttttct tt
//
LOCUS       DATXIG010000066         7069 bp    DNA     linear   BCT 28-DEC-2023
DEFINITION  TPA_asm: Streptococcus pyogenes strain MGAS11113
            SAMN08555799-rid19682583.denovo.066, whole genome shotgun sequence.
ACCESSION   DATXIG010000066 DATXIG010000000
VERSION     DATXIG010000066.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN08555799
            Sequence Read Archive: SRR6740495
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pyogenes
  ORGANISM  Streptococcus pyogenes
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 7069)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7069)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-OCT-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-OCT-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001918058.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001918058.2
            Genome Coverage       :: 368x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/12/2023 11:23:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 1,806
            CDSs (total)                      :: 1,769
            Genes (coding)                    :: 1,722
            CDSs (with protein)               :: 1,722
            Genes (RNA)                       :: 37
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1, 1 (5S, 23S)
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 47
            CDSs (without protein)            :: 47
            Pseudo Genes (ambiguous residues) :: 0 of 47
            Pseudo Genes (frameshifted)       :: 30 of 47
            Pseudo Genes (incomplete)         :: 20 of 47
            Pseudo Genes (internal stop)      :: 9 of 47
            Pseudo Genes (multiple problems)  :: 11 of 47
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7069
                     /organism="Streptococcus pyogenes"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN08555799-rid19682583.denovo.066"
                     /strain="MGAS11113"
                     /serotype="Emm28"
                     /isolation_source="normally sterile site"
                     /host="Homo sapiens"
                     /db_xref="taxon:1314"
                     /country="Finland"
                     /collection_date="2002"
                     /collected_by="National Public Health Laboratory"
     gene            complement(325..2901)
                     /locus_tag="VC122_001493"
     CDS             complement(325..2901)
                     /locus_tag="VC122_001493"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014635800.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YfhO family protein"
                     /protein_id="HEP5641410.1"
                     /translation="MKNNNKWIIAGLASFLFPLSIIFIILLSMGIYYNSDKTILASDA
                     FHQYVIFAQNFRNIMHGSDSFFYTFTSGLGINFYALMCYYLGSFFSPLLFFFNLTSMP
                     DAIYLFTLIKFGLIGLAACYSFHRLYPKINAFLMISISVFYSLMSFLTSQMELNSWLD
                     VFILLPLVVLGLNKLITENKTRTYYLSISLLFIQNYYFGYMIALFCILYALVCLLSLN
                     DFNKMFIAFVRFTAVSICAALTSALVILPTYLDLSTYGENLSPVKQLVTNNAWFLDIP
                     AKLSIGVYDTTKFNALPMIYVGLFPLMLSVIYFTLESIPLKIKLANACLLTFIIISFY
                     LQPLDLFWQGMHSPNMFLHRYAWSFSIVILLLACETLSRLKEVTQIKAGFAFIFLIIL
                     TSLPYSFSQQYNFLPLTLFLLSVFLLLGYTISLFSFRNSQIPPTFISAFILIFSLLES
                     GLNTYYQLQGINKEWGFPSRQIYNSQLKDINNLVNSVSKNSQPFFRMERLLPQTGNDS
                     MKFNYYGISQFSSVRNRLSSSLLDRLGFQSKGTNLNLRYQNNTIIMDSLLGIKYNLSE
                     GPLNKFGFTKLKTSGNTTLYQNHYSSPLAILTRNVYKDVNLNVNTLDNQTKLLNQLSG
                     KSLTYFNLQPAQLISGANQFNGQISAQASDYQNSVTLNYQINIPKHSQLYVSIPNIIF
                     SNPDAKEVRIQTDNHNFIYTTDNAYSFFDLGYFADAKVATFSFVFPKNKQISFKEPHF
                     YSLSIESYLEAMNSIKQKNVHTYAKSNTVITDYNSKTKGSLIFTLPYDKGWSAQKDGK
                     NLPVKKAQGGFLSVTIPKGKGRVILTFIPNGFKLGLSLSCVGIIAYMLLYKYIDIKSK
                     LL"
     gene            complement(2984..4603)
                     /locus_tag="VC122_001494"
     CDS             complement(2984..4603)
                     /locus_tag="VC122_001494"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015058862.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HEP5641411.1"
                     /translation="MLTVSDVSLRFSDRKLFDDVNIKFTAGNTYGLIGANGAGKSTFL
                     KILAGDIEPSTGHISLGPDERLSVLRQNHFDYEEERAIDVVIMGNEQLYNIMKEKDAI
                     YMKADFSEEDGVRAAELEGIFAELGGWEAESEASQLLQNLNIPEDLHYQNMSELANGD
                     KVKVLLAKALFGKPDVLLLDEPTNGLDIQSISWLEDFLIDFENTVIVVSHDRHFLNKV
                     CTHMADLDFGKIKLFVGNYDFWKQSSELAARLQADRNAKAEEKIKELQEFVARFSANA
                     SKSKQATSRKKMLDKIELEEIVPSSRKYPFINFKAEREMGNDFLTVENLSVTIDGEKI
                     IDNISFILRPGDKAAIIGQNDIQTTALMRALADDIDYEGTIKWGVTTSRSYLPKDNSK
                     DFATEESILEWLRQFASKGEDDDTFLRGFLGRMLFSGDEVKKSVIVLSGGEKVRVMLS
                     KLMLLKSNVLILDDPTNHLDLESISSLNDGIKDFKESVIFASHDHEFIQTIANHIVVI
                     SKNGVIDRIDETYDEFLDNPEVQARVAELWK"
     gene            complement(4682..5554)
                     /locus_tag="VC122_001495"
     CDS             complement(4682..5554)
                     /locus_tag="VC122_001495"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011018344.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YitT family protein"
                     /protein_id="HEP5641412.1"
                     /translation="MKKRGIDLLLVTIGSFITAIGFNTMFVDNHIASGGMVGIAVVIK
                     ALFGISPSLFLMASNIPLLLMCYFFLGKQNFIKTLYGSWIYPIAIRSTNSLPTLTHNQ
                     LLAAIFGGIICGIGLGMVFWGNSSTGGTGILTQILHKYSPLSLGVAMTIVDGISVLMG
                     FIALSADDVMYSTIGLFVIGYVISVMENGFDSSKNVMIISKDYQAIREYITTVMDRGV
                     TKLPIRGGYTTSDKIMLMAIVSSHELPTLQEKILEIDDTAFIVVMPAAQVMGRGFSLT
                     KQYKREDKDVLLPM"
     misc_binding    5677..5918
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00230"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="T-box leader; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="tRNA"
                     /db_xref="RFAM:RF00230"
     gene            5973..6995
                     /gene="trpS"
                     /locus_tag="VC122_001496"
     CDS             5973..6995
                     /gene="trpS"
                     /locus_tag="VC122_001496"
                     /EC_number="6.1.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008087735.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tryptophan--tRNA ligase"
                     /protein_id="HEP5641413.1"
                     /translation="MTKPIILTGDRPTGKLHLGHYVGSLKNRVFLQNENKYKMFVFLA
                     DQQALTDHAKESELIQESIGNVALDYLSVGLDPKQSTIFIQSQIPELAELSMYYMNLV
                     SLARLERNPTVKTEIAQKGFGESIPSGFLVYPVSQAADITAFKANLVPVGNDQKPMIE
                     QTREIVRSFNHTYHTDCLVEPEGIYPENEKAGRLPGLDGNAKMSKSLGNGIYLSDDAD
                     TVRKKVMSMYTDPNHIKIEDPGQIEGNMVFHYLDIFARKEDQADIEAMKEHYQRGGLG
                     DVKTKRYLLDILERELAPIRERRLEYAKDMGEVFRMLQEGSQKARTVAAKTLSEVKSA
                     MGINYF"
BASE COUNT         2462 a         1126 c         1270 g         2211 t
ORIGIN      
        1 cattaagttt aattacaaac attaactgaa gcttaatttt ttacattttg aaagcatgtc
       61 tcccatttaa tactgatgaa tataagatta ttgtaaggag tcaaaagatt gaaatcgtat
      121 cagcgatgat tattgtcatt tcaatctttg aaagagagta gtttaacatt aaccctcacg
      181 aaaaatctga cctttatatt atttgagttt attagtaaaa tcgataaaag catctcaagt
      241 attagaatat ttgtctagct cttgagatgc ttttagtata ttgactcatt tgccaaattg
      301 ataaaaaaat ctgatttttt tggactaaag taatttagac tttatatcta tgtacttata
      361 caaaagcata taagcgataa ttcctacgca agatagagat aaccctaatt taaaaccatt
      421 aggaataaag gtaaggataa cacgtccctt tcctttagga atagtaactg atagaaatcc
      481 tccttgtgct tttttgactg gaagattttt cccatctttt tgtgctgacc aacctttatc
      541 gtaaggaagt gtaaaaataa gagaaccttt cgtttttgaa ttataatcag tgattaccgt
      601 attactttta gcgtaagtat gaacattttt ttgtttaata ctattcattg cttcaaggta
      661 agattcaata gacaaactat aaaaatgagg ttccttaaaa ctaatttgtt tattttttgg
      721 aaaaacaaac gaaaatgtag caactttggc atcggcgaaa tatcctaaat caaaaaaaga
      781 gtaagcgtta tctgtagtat atatgaaatt atgattatct gtctgaatac gcacctcttt
      841 agcatcagga tttgaaaata taatattggg tatgctaaca tagagttgac tatgtttagg
      901 gatgttaatt tgataattaa gggtaacgga gttttgataa tcagaagctt gtgcagatat
      961 ttgtccgtta aattgattag caccagaaat aagttgagct ggctgtaagt taaaataggt
     1021 taaagatttc ccacttagtt ggttaagtaa tttggtttgg ttatcaaggg tattgacatt
     1081 tagattgaca tctttgtaaa cattacgtgt taatatagct aaagggctac tatagtgatt
     1141 ttgataaaga gtagtattcc cgctagtttt tagttttgta aatccaaatt tatttagagg
     1201 tccttcgcta agattatatt ttataccaag tagactgtcc ataataatag tattgttttg
     1261 gtatctaagg tttaaattcg tgcctttaga ctgaaatccc aatcgatcca ataaagaact
     1321 agatagtcta tttcttacag aggaaaattg tgaaatgccg taataattaa atttcatgct
     1381 atcgttccct gtttggggaa gtagtctttc cattctaaaa aaaggttgac tattttttga
     1441 cacagagttg acaaggttgt taatatcctt taattgacta ttatatatct gtcgtgatgg
     1501 gaatccccac tccttattaa ttccttgaag ctggtagtag gtgtttaacc ctgattcaag
     1561 aaggctaaag ataagtatga aagcagaaat aaaagtaggt gggatttgag aatttctaaa
     1621 cgaaaatagt gaaatagtat aacctaataa taaaaaaaca ctaagtaaaa aaagagttaa
     1681 aggtagaaaa ttatattgtt gagaaaagct ataaggaaga gatgtcagta taatgaggaa
     1741 aataaaagca aaacctgctt ttatttgagt cacttctttt agtcgagaga gagtctcaca
     1801 tgcgagtaat aggataacta tggaaaaaga ccaagcgtag cgatgcaaaa acatatttgg
     1861 tgagtgcatc ccctgccaaa aaagatcaag tggctgtagg taaaaactta ttataataaa
     1921 agttaacaag caggcattgg ctaattttat ttttaaaggg atactttcta aagtaaaata
     1981 aataacacta agcattaggg gaaataatcc tacgtaaatc ataggcagag cattaaactt
     2041 ggtagtatcg tacactccta ttgagagctt agcaggtata tccaaaaacc aagcattgtt
     2101 cgtaactaac tgttttaccg gggatagatt ctctccataa gttgacaaat ctagataggt
     2161 aggaagtatt accagagcac ttgttaaagc agcacatatt gacacagctg taaacctaac
     2221 aaaagcgata aacattttgt taaaatcatt gagacttaaa agacaaacta aggcgtaaag
     2281 aatacaaaaa agagcaatca tgtagccaaa gtagtaattt tgaatgaata ataatgatat
     2341 cgaaagataa taagttctgg ttttattttc tgtgataagt ttatttaatc caagtacaac
     2401 aagtggaaga agaatgaaaa catctaacca agaatttagt tccatttgac ttgtcaagaa
     2461 gctcattaag ctataaaaaa ctgagatgga aatcatcaag aaagcattga tttttggata
     2521 taatctatga aaagaatagc atgcagctaa tcctattaac ccaaatttta tcaaggtaaa
     2581 caaatagata gcatctggca tagaggttaa attaaaaaag aaaagtaatg gagaaaagaa
     2641 actgccaaga taataacaca ttaaagcata aaaatttatc cctagtccgc ttgtaaaggt
     2701 ataaaaaaaa ctatcagaac cgtgcatgat gttacgaaag ttctgcgcaa aaataacata
     2761 ctgatgaaaa gcatcactag ctagaattgt tttatcacta ttataataaa tgcccatcga
     2821 tagaaggatg ataaatataa tactaagagg gaacaaaaaa ctagcaagtc cagcaattat
     2881 ccatttatta ttatttttca taaattatat ggtctttcgt ttacaaattt taaaaaagag
     2941 gctaggaaga gatcttctta gccacctcat ttatcataag gttttatttc caaagttcag
     3001 caactcgggc ttggacttct ggattgtcaa ggaattcgtc ataagtttca tctatacggt
     3061 cgataacacc attttttgaa ataacaacga tgtggttagc aatcgtttga ataaattcat
     3121 ggtcatgact ggcaaagata acagattctt tgaagtcttt gatcccatca ttaagacttg
     3181 aaatagattc caaatccaag tgatttgttg gatcatcaag aataagaacg tttgatttca
     3241 atagcatcaa cttagaaagc atgacacgca ctttttcgcc accggaaaga acgataacag
     3301 attttttaac ttcgtctcca gagaaaagca tccgtcctag gaaaccacgt aaaaaagtat
     3361 cgtcatcttc accttttgag gcaaattgac gtaaccattc aagaatagat tcttccgtgg
     3421 caaaatcttt tgaattgtct ttaggcaagt aagaacgact tgttgtcact ccccatttaa
     3481 tggttccttc gtagtcaata tcatctgcta aagcacgcat aagagcggtt gtttgaatat
     3541 cattttgacc aataatagca gctttgtcac ctggacgtaa aatgaaactg atattatcaa
     3601 tgattttctc cccatcgatc gttacagaaa gattttcaac agtgaggaag tcattcccca
     3661 tttcacgctc tgctttaaaa ttaataaatg ggtatttacg gcttgaggga acaatttctt
     3721 ctaattcaat cttatcaagc atttttttac gagaggttgc ttgttttgat ttagatgcgt
     3781 ttgctgagaa acgggcaacg aattcttgta attctttgat tttttcttcg gctttagcat
     3841 tgcggtcagc ttgtaaacga gcggctagct cagaagattg tttccagaaa tcgtagttac
     3901 ccacaaagag tttgatttta ccaaagtcaa ggtcggccat gtgagtacat actttattca
     3961 agaagtgacg gtcatgggaa acaacgatga ctgtattttc aaaatcaatc aagaaatctt
     4021 ctaaccatga aatagattga atatcaagac cattggttgg ctcgtcaagt aataaaacgt
     4081 caggcttacc aaagagcgct ttagcaagaa ggactttcac tttatctcca ttagccagtt
     4141 cactcatatt ttggtagtga aggtcttctg gaatatttag attttggaga agttgagaag
     4201 cttcactttc tgcctcccag ccacctaatt cagcaaagat accttctagc tcagcggcac
     4261 gaaccccatc ttcttctgaa aaatctgctt tcatatagat agcgtctttt tctttcatga
     4321 tattgtaaag ttgctcatta cccatgatta caacatcaat cgcacgttct tcttcataat
     4381 caaaatgatt ttggcgaaga acagataagc gttcgtcagg tcctaaagag atatgaccag
     4441 tacttggttc aatatctcct gcaaggattt taagaaaagt tgattttccg gcaccattcg
     4501 caccgattaa gccgtaagta ttgccggctg tgaatttgat attaacatca tcaaatagtt
     4561 ttcgatcact aaagcgtagt gatacatcag aaactgtaag caatttgtca cctcatttta
     4621 tttatctaag cttcattgta cctaaaatag tccccttttt caatcagaaa agggctaatc
     4681 actacatagg caataaaacg tctttgtctt cacgtttgta ttgtttagtc aaactaaatc
     4741 ctcttcccat aacttgagct gctggcatga caacaataaa ggctgtatcg tcaatttcga
     4801 ggattttttc ttggagagtg ggtaattcgt gacttgatac aatagccatt aacataattt
     4861 tatcagaagt agtataacca ccgcgaatag gaagtttagt cactccacga tccataacag
     4921 ttgtgatata ttctcgaata gcttgataat ctttggaaat aatcataaca tttttggatg
     4981 agtcaaagcc attttccata acgctaatca cataaccaat tacgaaaagt ccaatcgtag
     5041 aatacatcac gtcatctgca gaaagtgcaa taaatcccat aaggacacta ataccatcta
     5101 caattgtcat tgcaactcca agagataaag gagagtattt atgaagtatt tgtgttagaa
     5161 ttccagttcc tcctgtcgaa gagtttcccc aaaaaaccat tcctaaacca atgccacaga
     5221 taataccacc aaaaatagct gctaacaact gattgtgtgt cagagtaggt aatgaattgg
     5281 ttgaacggat ggcaatagga tatatccaag aaccataaag tgttttaata aaattttgtt
     5341 ttccaagaaa gaagtaacac attaataata agggaatatt actagccatt aaaaaaagtg
     5401 acggactaat gccaaataga gcttttataa caactgcaat accaaccata ccccctgatg
     5461 caatatgatt gtcgacaaac attgtattga aaccaatagc tgttatgaat gagccaattg
     5521 tgacaagtag taaatcaatc cctcgttttt tcatgctttt aatctccttt ataactggtt
     5581 aaaaatagta gactcattat ctcttaaatg tactgagacc gtcaagtcat attttcaaaa
     5641 aatctggtga acttgacaag aagtttaaaa tttcatagaa taaatttgaa ttagaagagt
     5701 attcttttca tgtttttaga gagttagtgg ttggtgcaag ctaacaacag aaaaggtgaa
     5761 atggactaat gattatttga aaggaaacca taactttcag gttggcaccc cttaccgtgc
     5821 agcttctttc attgagaaga ttgagatagt atctcgtttt gataaagcag atgctaattg
     5881 aggtggtacc gcgtattact tgtaataacg ccctcacgtt ttaatagcgt ggggactttt
     5941 tgctatatca agagatagtt agggagaaaa tgatgacaaa acctattatt ttaactggag
     6001 atagaccaac tggaaaatta catttaggtc attatgtcgg aagtcttaaa aatcgtgtct
     6061 ttttacaaaa tgaaaacaag tataagatgt ttgttttctt ggccgatcaa caggcactga
     6121 cggatcatgc taaagaatcc gaattaattc aagaatctat tggaaatgtt gccttggatt
     6181 acctctctgt cggtttggat ccgaagcaat caacgatttt tattcaaagt cagattccag
     6241 agctagctga attgagcatg tattatatga atctggtatc attagcacgt ttggaaagaa
     6301 atcccactgt taaaaccgaa attgctcaga aaggttttgg cgaaagtatt ccatctggtt
     6361 ttttggttta tccagtatca caggccgctg atattacagc atttaaagcg aatttagtac
     6421 ctgtaggtaa cgatcagaaa ccgatgattg aacaaacacg tgaaattgtg agaagtttta
     6481 atcatactta ccatacagac tgtttagtag aacctgaagg tatttatcca gaaaatgaaa
     6541 aagctggacg cttacctggt cttgatggca atgccaaaat gtctaaatca ttgggaaatg
     6601 gaatctatct ttcagatgat gcagataccg ttcgcaaaaa agtgatgagc atgtatactg
     6661 atccaaatca tattaaaata gaagatcctg gtcaaattga agggaatatg gtctttcatt
     6721 atttggatat ttttgctaga aaagaagatc aagctgatat cgaagcaatg aaagagcatt
     6781 atcaaagagg tggtttagga gatgtgaaaa cgaaacgcta ccttctagat attttagaac
     6841 gtgaattagc acctattcgt gaaagacgtt tggagtacgc taaagatatg ggagaggtgt
     6901 tccgtatgtt acaagaaggt agtcaaaaag caagaactgt ggcagccaag actttatcag
     6961 aagtgaagtc agcaatgggt attaattatt tttaatgttt tagagaaaaa gctaagttag
     7021 taatgataag ataattacta ttatcaaagc aggacctaat ttgtcattt
//
LOCUS       DATXIG010000067         7038 bp    DNA     linear   BCT 28-DEC-2023
DEFINITION  TPA_asm: Streptococcus pyogenes strain MGAS11113
            SAMN08555799-rid19682583.denovo.067, whole genome shotgun sequence.
ACCESSION   DATXIG010000067 DATXIG010000000
VERSION     DATXIG010000067.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN08555799
            Sequence Read Archive: SRR6740495
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pyogenes
  ORGANISM  Streptococcus pyogenes
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 7038)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7038)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-OCT-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-OCT-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001918058.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001918058.2
            Genome Coverage       :: 368x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/12/2023 11:23:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 1,806
            CDSs (total)                      :: 1,769
            Genes (coding)                    :: 1,722
            CDSs (with protein)               :: 1,722
            Genes (RNA)                       :: 37
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1, 1 (5S, 23S)
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 47
            CDSs (without protein)            :: 47
            Pseudo Genes (ambiguous residues) :: 0 of 47
            Pseudo Genes (frameshifted)       :: 30 of 47
            Pseudo Genes (incomplete)         :: 20 of 47
            Pseudo Genes (internal stop)      :: 9 of 47
            Pseudo Genes (multiple problems)  :: 11 of 47
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7038
                     /organism="Streptococcus pyogenes"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN08555799-rid19682583.denovo.067"
                     /strain="MGAS11113"
                     /serotype="Emm28"
                     /isolation_source="normally sterile site"
                     /host="Homo sapiens"
                     /db_xref="taxon:1314"
                     /country="Finland"
                     /collection_date="2002"
                     /collected_by="National Public Health Laboratory"
     gene            complement(31..303)
                     /locus_tag="VC122_001497"
     CDS             complement(31..303)
                     /locus_tag="VC122_001497"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002262439.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Veg family protein"
                     /protein_id="HEP5641414.1"
                     /translation="MSDAFTDVAKMKKIKEDIRAHEGQLVELTLENGRKREKNKIGRL
                     IEVYSSLFIIEYSDSSDTPGAIDNSYVESYTYSDILTEKTLIRYLD"
     gene            complement(320..1687)
                     /gene="dnaB"
                     /locus_tag="VC122_001498"
     CDS             complement(320..1687)
                     /gene="dnaB"
                     /locus_tag="VC122_001498"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010922787.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replicative DNA helicase"
                     /protein_id="HEP5641415.1"
                     /translation="MRLPEVAELRVQPQDLLAEQSVLGSIFISPDKLIAVREFISPDD
                     FYKYAHKIIFRAMITLSDRNDAIDATTIRTILDDQDDLQSIGGLSYIVELVNSVPTSA
                     NAEYYAKIVAEKAMLRDIIARLTESVNLAYDEILKPEEVIAGVERALIELNEHSNRSG
                     FRKISDVLKVNYEALEARSKQTSNVTGLPTGFRDLDKITTGLHPDQLVILAARPAVGK
                     TAFVLNIAQNVGTKQKKTVAIFSLEMGAESLVDRMLAAEGMVDSHSLRTGQLTDQDWN
                     NVTIAQGALAEAPIYIDDTPGIKITEIRARSRKLSQEVDGGLGLIVIDYLQLITGTKP
                     ENRQQEVSDISRQLKILAKELKVPVIALSQLSRGVEQRQDKRPVLSDIRESGSIEQDA
                     DIVAFLYRDDYYRKEGDDAEEAVEDNTIEVILEKNRAGARGTVKLMFQKEYNKFSSIA
                     QFEER"
     gene            complement(1717..2169)
                     /gene="rplI"
                     /locus_tag="VC122_001499"
     CDS             complement(1717..2169)
                     /gene="rplI"
                     /locus_tag="VC122_001499"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002991410.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L9"
                     /protein_id="HEP5641416.1"
                     /translation="MKVIFLADVKGKGKKGEIKEVPTGYAQNFLIKKNLAKEATSQSI
                     GELKGKQKAEEKAQAEILAEAQAVKAVLDEDKTRVQFQEKVGPDGRTFGSITAKKISE
                     ELQKQFGVKVDKRHIVLDHPIRAIGLIEVPVKLHKEVTAEIKLTITEA"
     gene            complement(2166..4142)
                     /locus_tag="VC122_001500"
     CDS             complement(2166..4142)
                     /locus_tag="VC122_001500"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003045829.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DHH family phosphoesterase"
                     /protein_id="HEP5641417.1"
                     /translation="MKKFRFETIHLIMMGLILFGLLALCVSIMQSKILILLAIFLVLL
                     FVVALLWYQKEAYHLSDLAHIELLNEQTEDNLKTLLDNMPVGVVQFDQETNAVEWYNP
                     YAELIFTTEEGFIQNSLIQQIITEKRREDISQTFEVSGNKYTSYIDVSSGIFYFFDSF
                     VGNRQLADASMLRPVVGIISVDNYDDITDDLSDADTSKINSFVANFIDEFMESKRIFY
                     RRVNMDRYYFFTDFKTLNDLMDNKFSVLEEFRKEAQDAQRPLTLSIGISFGEENHSQI
                     GQVALENLNIALVRGGDQIVIRENADHTNPIYFGGGSVSTVKRSRTRTRAMMTAISDR
                     IKMVDNVFIVGHRKLDMDALGSAVGMQFFAGNIIENSFAVYNPDEMSPDIERAIERLQ
                     ADGKTRLISVSQAMGLVTPRSLLVMVDHSKISLTLSKEFYEQFQNVIVVDHHRRDDDF
                     PDNAILTFIESGASSAAELVTELIQFQNAKKRLNKIQASVLMAGIMLDTKNFSTRVTS
                     RTFDVASYLRSKGSDSVEIQNISATDFEEYKQINEIILQGERLGDSIIVAAGEKNHLY
                     SNVIASKAADTILSMAHVEASFVLVETASHKIAISARSRSKINVQRVMEKLGGGGHFN
                     LAACQLTDISLPQAKHLLLKTINMTMKETGEVES"
     gene            complement(4232..6130)
                     /gene="mnmG"
                     /locus_tag="VC122_001501"
     CDS             complement(4232..6130)
                     /gene="mnmG"
                     /locus_tag="VC122_001501"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002982082.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA uridine-5-carboxymethylaminomethyl(34)
                     synthesis enzyme MnmG"
                     /protein_id="HEP5641418.1"
                     /translation="MTHEFTESYDVIVIGAGHAGVEASLATSRMGCKTLLATINLDML
                     AFMPCNPSIGGSAKGIVVREIDALGGEMGKNIDKTYIQMKMLNTGKGPAVRALRAQAD
                     KSLYAREMKHTVEKQANLTLRQTMIDDILVEDGRVVGVLTATGQKFAAKAVVVTTGTA
                     LRGEIILGELKYSSGPNNSLASVTLADNLKKLGLEIGRFKTGTPPRVKASSINYDQTE
                     IQPGDDKPNHFSFMSKDAEYLKDQIPCWLTYTNQTSHDIINQNLYRAPMFSGIVKGVG
                     PRYCPSIEDKIVRFADKERHQLFLEPEGRDTEEVYVQGLSTSLPEDVQKDLIHSIKGL
                     EKAEMMRTGYAIEYDIVLPHQLRATLETKLISGLFTAGQTNGTSGYEEAAGQGLIAGI
                     NAALKVQGKPELILKRSDAYIGVMIDDLVTKGTLEPYRLLTSRAEYRLILRHDNADMR
                     LTEIGRDIGLVDDERWKAFEIKKNQFDNELKRLNSIKLKPVKATNDRVQELGFKPLTD
                     AMTAKEFMRRPEIDYATAVSFVGPAAEDLDAKIIELLETEIKYEGYIRKALDQVAKMK
                     RMEEKRIPANIDWDAIDSIATEARQKFKKINPETIGQASRISGVNPADISILMIYLEG
                     NGKAHRKY"
     gene            complement(6254..6571)
                     /locus_tag="VC122_001502"
     CDS             complement(6254..6571)
                     /locus_tag="VC122_001502"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011529140.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX domain-containing protein"
                     /protein_id="HEP5641419.1"
                     /translation="MIVRNGKNFLTRDAADQYYTIGGTSLVGEKTHETVLRETLEEVG
                     IRTKVNQLAFVVENHFDIDDVFWHNIEFHYLVSPLEDPKLEMIENASDRFVNGFQSIN
                     WIK"
BASE COUNT         2151 a         1479 c         1087 g         2321 t
ORIGIN      
        1 tgtaaagaaa aagcctctat gaggcaaaaa tcaatctaaa taacgaatta atgttttttc
       61 tgtcaaaata tctgaatagg tgtatgattc gacataagaa ttatcaattg ctcctggagt
      121 atcagagcta tcactatact caatgataaa gagcgaactg taaacctcaa taagcctacc
      181 aattttattt ttttcccgtt tccgtccatt ctcaagggtt aattctacta actgtccttc
      241 atgggctctt atgtcctctt taattttttt catttttgca acatctgtaa atgcatcgct
      301 cattttttgt ctctctttct tatctttctt caaactgggc tatacttgag aatttgttgt
      361 attctttttg aaacatcagt ttgactgttc cacgcgcccc agctctattt ttctcgagga
      421 taacttcaat tgtgttatct tcaacagctt cttcagcatc atcaccttct ttacggtaat
      481 aatcgtcccg gtataagaag gctacaatat cggcatcctg ctcaatagat cctgattcac
      541 gaatatctga taaaactggt cgtttatctt gcctttgctc aacgccacga gaaagctgac
      601 ttagggcaat aactggtact ttcaattctt tagctaggat tttaagctgt cttgaaatat
      661 ctgaaacctc ttgctgacga ttttcgggtt tagttcctgt aatcaactgt aagtagtcaa
      721 ttacgataag acctaaacca ccatccactt cttgagacaa tttccgtgat cttgcgcgga
      781 tttcagtaat tttaatcccg ggcgtatcgt caatataaat cggtgcttct gccaaagctc
      841 cctgagcaat tgttacatta ttccaatcct gatctgtgag ttgccctgtt cttaaactgt
      901 gcgaatcaac cattccttct gctgcaagca tacgatctac taaactttca gcacccattt
      961 ccaaagaaaa aatagcaaca gtcttttttt gcttagtccc cacattttgc gcaatattaa
     1021 gaacaaaggc agtcttcccc actgctggcc gagcagctaa aataactaat tgatctgggt
     1081 gtaaacctgt tgtaatcttg tcaaggtctc taaaaccagt tggtaaacct gtaacatttg
     1141 aagtctgctt agaacgtgct tctaaagcct cgtaattaac ttttagcaca tctgaaattt
     1201 tgcgaaaccc actacgatta ctatgttcat tgagttcaat taaagctctc tcaactccag
     1261 cgataacctc ttctggtttt aaaatttcat cataagctag gttgacagat tctgtcaacc
     1321 tagcaataat atcacgcaac atagctttct cagctacaat tttagcataa tattctgcat
     1381 tagcactagt tgggacacta ttaactagtt caacaatata ggataagcca ccaatacttt
     1441 gcagatcatc ttgatcatct aggattgttc ttatagtggt tgcatcaatg gcatcattac
     1501 gatcgctgag ggtaatcatt gcccgaaaga taattttatg agcgtactta taaaaatcgt
     1561 ctggactgat aaattctctc actgcaatca gcttatcagg tgagataaag attgacccaa
     1621 gaacagattg ctctgctagt aaatcttggg gttgaactcg taattcagct acttcaggca
     1681 acctcataac ctccaaatct tgtgatgttt tttatattat gcttctgtga tagtcaattt
     1741 aatctcagct gttacttcct tatgtaattt cacaggaact tcaattagac caatggcgcg
     1801 gataggatga tctaaaacaa tatgacgttt atcaactttt acaccaaatt gcttttgcaa
     1861 ttcttctgaa atctttttag ctgtgatgga accaaatgta cgtccatctg gaccaacttt
     1921 ttcttgaaat tgaacacgtg ttttgtcctc atccaaaaca gccttgacag cttgagcttc
     1981 tgctaatatt tcagcttgag ctttttcttc agctttttgt tttcctttta attcaccgat
     2041 gctttgacta gttgcttctt tagctaaatt tttcttaata aggaaatttt gagcatagcc
     2101 tgttgggacc tctttgattt ctcctttttt accttttcct tttacatctg ctaaaaaaat
     2161 aactttcatg attctacttc tcctgtttct ttcattgtca tattaatagt tttcaataat
     2221 aagtgcttcg cttggggaag actgatatcc gttaactgac aggcagcaag attaaagtgg
     2281 cctcctccac ctaatttttc catcacacgt tgaacattga ttttactacg acttctagct
     2341 gaaatagcaa tcttatgaga agctgtttca actaatacaa agctagcctc aacgtgagcc
     2401 attgacagta tcgtatctgc agctttacta gcaataacat tgctataaag atgatttttt
     2461 tctcctgcag ctacaatgat actgtcacca agacgttctc cttgtaaaat aatttcattt
     2521 atttgcttat attcttcaaa atctgttgcg gatatatttt gaatttcaac actatcactt
     2581 cctttgcttc ttaggtaact agcgacatca aaggtacgac ttgtcactcg cgtagaaaaa
     2641 tttttagtat caagcataat acctgccatt aaaacactgg cttggatctt atttaaacgt
     2701 tttttagcat tttgaaattg aattaactca gtgacgagct ccgcagcact acttgctcca
     2761 ctttcaataa aagttaagat agcattatca ggaaaatcat catctcttct gtgatgatca
     2821 actacaataa cattttgaaa ctgttcataa aattctttag aaagtgttaa agatatctta
     2881 gaatggtcaa ccattactaa aagtgatctt ggagtcacta aacccattgc ttgagaaaca
     2941 ctgattagac gagtctttcc gtcagcctgc aatcgctcaa tagccctttc aatatctgga
     3001 ctcatctcat ctggattgta aacggcaaaa ctattttcaa taatgttacc tgcgaaaaat
     3061 tgcataccaa cagctgaacc tagtgcatcc atatcaagtt tcctatgtcc aacaataaaa
     3121 acattgtcca ccatcttgat tcgatctgaa atagctgtca tcatagcacg ggtacgtgtt
     3181 cttgaacgtt taactgtaga aacagaccct cccccgaaat aaattggatt cgtatgatcc
     3241 gcattttcac gaatgacaat ctgatcacca ccacgaacaa gcgcaatatt aagattctca
     3301 agagctacct gtccaatctg gctatgattt tcttcaccaa aagaaattcc tatgctgagt
     3361 gttaaagggc gttgagcatc ttgagcttct ttacgaaact cttctaagac agaaaattta
     3421 ttatccatca aatcatttaa tgttttaaag tctgtaaaga aatagtagcg atccatatta
     3481 acacgacggt aaaaaatccg ttttgactcc ataaactcat caatgaagtt agctacaaat
     3541 gaattaattt ttgaggtatc tgcatctgat aaatcatcag tgatatcatc ataattatca
     3601 accgaaataa tgccaacaac cggtcttaac atactagcat cagctaattg cctgtttccc
     3661 acaaagctat caaaaaaata aaaaattcct gatgatacat caatataaga agtgtattta
     3721 ttaccagaaa cttcaaatgt ttgagaaata tcttcacgac gtttttccgt aataatttgc
     3781 tgaatcaaac tgttttgtat aaaaccctct tcagttgtaa aaattaattc tgcataagga
     3841 ttataccatt ctacagcgtt ggtctcttga tcaaattgaa ctactccaac aggcatatta
     3901 tcaagtagcg tttttaggtt atcttctgtt tgttcattta agagctcaat atgagctaag
     3961 tctgataagt gataagcttc tttttgatac cataaaagag caacaacaaa tagcaaaacg
     4021 agaaaaatag ctaataatat taatattttt gattgcataa tactgacaca aagtgccagt
     4081 agtccaaata aaatcaatcc catcataatc aaatgaatgg tttcaaaacg aaattttttc
     4141 atcttataac ctcttaagca cacattatac cataaaaaag ccctgaaata cagagcttcc
     4201 acttgctttg agattacaat ttcatttcca tttaatattt tcgatgagct tttccgtttc
     4261 cttctaaata aatcatcaag atactgatgt ccgcaggatt gactcctgaa atacgactag
     4321 cttgtccaat tgtttcagga ttaattttct taaatttctg acgcgcctca gttgcaatag
     4381 aatcaatagc atcccaatca atatttgcag gaatacgttt ttcttccatg cgtttcatct
     4441 tagcaacctg atctaaagct ttgcgaatat agccttcgta cttgatttct gtttctaaaa
     4501 gttcaatgat tttagcatct aaatcttcag ccgcaggacc aacaaaagag actgcagtcg
     4561 cataatcaat ttctgggcga cgcataaatt ctttagctgt catagcatcc gtcaatggtt
     4621 taaaaccaag ttcctgcaca cgatcgttgg ttgcttttac tggttttaat ttgatactgt
     4681 tcagacgttt taattcgtta tcaaattggt ttttcttaat ttcgaaggcc ttccaacgct
     4741 catcatcaac caacccaatg tcgcgaccaa tctctgtcaa acgcatatca gcgttgtcat
     4801 gacgtaaaat caaacgatac tcagctcgcg atgtcaacaa tcgataaggt tccaaggttc
     4861 ctttggtaac caaatcgtca atcataacgc caatataagc atcgctccgt ttcaaaataa
     4921 gttctggctt accttgaact ttcaaagcag cattgattcc agcaataagt ccttggccag
     4981 cagcctcttc atacccagaa gttccattag tttgcccagc cgtaaagagt cctgaaatta
     5041 gcttggtttc aagtgtggca cgtagctggt gaggtaaaac aatatcatat tcaatagcat
     5101 aaccagtgcg catcatttca gccttttcaa gtcctttgat cgaatgaatc aaatcttttt
     5161 ggacatcttc tggaagactc gttgacaacc cttgaacata cacttcttct gtatcacgcc
     5221 cctctggctc taagaaaagt tgatgacgtt ctttatcagc aaagcgaaca atcttatctt
     5281 caatagatgg gcagtaacga gggcctacac ctttaacaat gccagaaaac atcggtgctc
     5341 tgtaaaggtt ctgattaatg atatcatgac tagtttgatt cgtataagtc aaccagcagg
     5401 gaatttgatc tttgagatac tcagcatctt ttgacataaa tgaaaaatga ttaggtttgt
     5461 catcacctgg ctgaatttcc gtttgatcgt aattaataga agatgctttg acacgaggag
     5521 gcgttcctgt tttgaatctg ccaatttcta agccgagttt ttttaaatta tctgctaaag
     5581 ttactgatgc caaactatta ttaggcccag aagaatattt gagttctcct aaaataattt
     5641 cgccacgcag agctgttcct gttgtcacta caactgcctt agctgcaaat ttttgtcctg
     5701 tggctgttaa aacacctaca actcgtccat cttcaactag aatatcatca atcatcgttt
     5761 ggcgaagagt caaattagcc tgtttttcaa ccgtatgttt catttcacgc gcatacaggg
     5821 atttatctgc ttgagctcgg agagcgcgta cagctggtcc cttacctgta ttaagcattt
     5881 tcatctggat ataggtttta tcaatatttt tacccatttc gccgccaaga gcatcaatct
     5941 ctctaaccac tatccctttg gcagatcctc caatagaagg attacacggc ataaaagcca
     6001 acatatcaag gttgattgtt gccaataaag ttttacaacc catacgacta gtggccaaac
     6061 tggcttcaac accagcatga cctgcaccta tgacaatgac gtcatagctt tctgtaaatt
     6121 catgtgtcat ttttttaaaa tcctttattt cagtctattc atttaaaatc atacgcttta
     6181 gtttagcctt caatgcggac aatatgtcct ggccatttag ccaactcggt ttgtaaaaac
     6241 tcaggaacaa gattcatttg atccagttga ttgattggaa tccattcaca aacctgtcgc
     6301 ttgcattctc tatcatttct agtttagggt cttctaaggg gcttaccaaa taatgaaatt
     6361 caatattatg ccagaaaaca tcatcgatat caaaatgatt ctcaaccaca aaagccagtt
     6421 gattaacctt agttcgaata ccaacttcct ctaaggtttc acgtaaaaca gtttcatgcg
     6481 ttttttcacc taccaaactt gtaccaccaa tggtgtaata ttggtcagct gcatctctag
     6541 ttaaaaaatt cttaccattt ctaacaatca agcactagct cgtaaactaa agttatctga
     6601 cctctagtga ttctaaatcc cagtcctatt agccatctca attctagagc taaaccataa
     6661 aaagggccaa aaccctagaa gattgcagga caaaaaaacc tacaactttc atgagctttg
     6721 accatcattc cgattgttat tatctatttt aaaaaaatga ttagtgattg tcaatattga
     6781 tttcatcaca gacctttaca gtaatgtcaa tgtttttaca tctctgtcaa tctattgtaa
     6841 tgtttttaaa atgattacaa aagtatcact atgtctaatc tagcgaggcg agaaataaaa
     6901 atgtcatcag tattatcata cgattttttg atatcttcta aagtagtaaa atacagatac
     6961 cgttgtttct aagtctacaa gtaagaacgc cgataacaaa accataaaag tataactaga
     7021 aatttccaat aatttttt
//
LOCUS       DATXIG010000068         6937 bp    DNA     linear   BCT 28-DEC-2023
DEFINITION  TPA_asm: Streptococcus pyogenes strain MGAS11113
            SAMN08555799-rid19682583.denovo.068, whole genome shotgun sequence.
ACCESSION   DATXIG010000068 DATXIG010000000
VERSION     DATXIG010000068.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN08555799
            Sequence Read Archive: SRR6740495
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pyogenes
  ORGANISM  Streptococcus pyogenes
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 6937)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6937)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-OCT-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-OCT-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001918058.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001918058.2
            Genome Coverage       :: 368x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/12/2023 11:23:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 1,806
            CDSs (total)                      :: 1,769
            Genes (coding)                    :: 1,722
            CDSs (with protein)               :: 1,722
            Genes (RNA)                       :: 37
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1, 1 (5S, 23S)
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 47
            CDSs (without protein)            :: 47
            Pseudo Genes (ambiguous residues) :: 0 of 47
            Pseudo Genes (frameshifted)       :: 30 of 47
            Pseudo Genes (incomplete)         :: 20 of 47
            Pseudo Genes (internal stop)      :: 9 of 47
            Pseudo Genes (multiple problems)  :: 11 of 47
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6937
                     /organism="Streptococcus pyogenes"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN08555799-rid19682583.denovo.068"
                     /strain="MGAS11113"
                     /serotype="Emm28"
                     /isolation_source="normally sterile site"
                     /host="Homo sapiens"
                     /db_xref="taxon:1314"
                     /country="Finland"
                     /collection_date="2002"
                     /collected_by="National Public Health Laboratory"
     gene            complement(85..618)
                     /locus_tag="VC122_001503"
     CDS             complement(85..618)
                     /locus_tag="VC122_001503"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010922785.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HEP5641420.1"
                     /translation="MVNRKENTKQAILKAMVMLLKTESFDDITTVKLSKRAGISRSSF
                     YTHYKDKYEMIDYYQQTFFHKLEYIFEKKYQNKEQAFLEVFEFLQREQLLSSLLSANG
                     TKEIQAFIINKVRLLITTDLQDKFSTEELSQTEKEYQSIYLAHAFFGVCQSWIAKGKK
                     ESPQEMTQFVLKMLTST"
     gene            753..3026
                     /locus_tag="VC122_001504"
     CDS             753..3026
                     /locus_tag="VC122_001504"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002994798.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YhgE/Pip domain-containing protein"
                     /protein_id="HEP5641421.1"
                     /translation="MLEELKTLIKNPKLMITMIGVALVPALYNLSFLGSMWDPYGRVN
                     DLPIAVVNHDKPAKRADKSLTIGNDMVDKMSKSKDLEYHFVSAKQAQEGLKEGDYYMV
                     ITLPEDLSQRAATLLNPEPQKLTIRYQTSKGHGMVAAKMGETAMAKLKESVSQNITKT
                     YTSAVFSSMTDLQSGLKEASAGSQALASGAKTAQAGSQTLSTNLAALTGASQQFQQGT
                     GRLTSGLTTYTDGVNQVKNGLGTLSTDIPNYLNGVSRLSQGASQLNQGLSQLTQATTL
                     SDEKAKGIQSLIVGLPVLNQGIQQLNTELSTLQPPNLNADELGNSLGAIAQAAKQVIA
                     EETAAQNEQLSALQATSVYQSLTAEQQGELAAALSQSDKSQTVSAAQTILSSVQTLST
                     GLQTLSQGNSLAQLDQLKAAVSQIASQSNQALPGASSALSELSMGLSKVNGSLNQQVL
                     PGSNQLTTGLAQLNRYNTVIGSGVIKLSEGANALSSKSGELLDGSHQLSEGATKLADS
                     SSQLSQGGHQLTSGLTELSTGLSTLNGSLAKASQQLSLVSVTDKNAKAVAKPLVLNEK
                     DKDGVKTNGIGMAPYMIAVSLMVVALSTNVIFANSLSGRPVKDKWDWAKQKFVINGFI
                     STMGSIVLYLAIQLLGFEARYGMETLGFIMLSGWTFMALVTALVGWDDRYGSFASLVM
                     LLLQVGSSGGSYPIELSGAFFQKLHPFLPMTYVVSGLRQTISLSGHIGVEVKVLTGFL
                     LAFMVLALLIYRPKKTV"
     gene            complement(3143..4216)
                     /locus_tag="VC122_001505"
     CDS             complement(3143..4216)
                     /locus_tag="VC122_001505"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011285298.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4097 family beta strand repeat protein"
                     /protein_id="HEP5641422.1"
                     /translation="MKNISRKCFMTSVVCIVLGAILLGAGYATGGLQDIKHQTAPKKV
                     IQTFDHVTALDIDTNFSTITVETGPVKKPTVTYYTHSKFIDPLEIKIENETLHISEKP
                     RDVIITGGIELIGFGLNKSDRNHNYRTTTITLPKGTSLKELKGSSMPQATISDLTVQD
                     LEFSGGLTLLNTRIKKGKVFGTFNATDSLLANLELKADYSLSSLTNSSLENGTISLGH
                     GQLTTKNTNLKSVTIQSLSPGGVETEETSLENVTFTVTSEQENEETNDNGAIFTAHAL
                     TLKGTNTISGGNVAVDITLTDPKTTAYKTKTEDGKILLGSQLTPAKIGKESTSDVISY
                     VAENKAATGNLTVNLNKGDITIK"
     gene            complement(4213..4800)
                     /locus_tag="VC122_001506"
     CDS             complement(4213..4800)
                     /locus_tag="VC122_001506"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011889248.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1700 domain-containing protein"
                     /protein_id="HEP5641423.1"
                     /translation="MTRTEYLAELDKYLRKLPREDYHEALEYYIEYFDEAGPDKESQV
                     IDDLGNPKEAASEIISNVLGKHLATPEKTPKNRATIIGLTILSLFAAPIALPVLLALI
                     LFIIACLMLGLTAIFAAYIFGLAGILVAGVTLFESLTLIGSSIPAQAMGIGSALLCFG
                     GGLLIWIIATALLRFSGRAFVAFIKWISHKKGAQA"
     gene            complement(4787..5113)
                     /locus_tag="VC122_001507"
     CDS             complement(4787..5113)
                     /locus_tag="VC122_001507"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018376434.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PadR family transcriptional regulator"
                     /protein_id="HEP5641424.1"
                     /translation="MYFPVSATLIEFLILAIVEKNDSYGYDISQTIKLVANIKESTLY
                     PILKKLEKAGFLTTYSQEHQGRKRKYYAVTSSGRAQLIFLKKEWQSYKFALDGIIEGS
                     LRHDKD"
     gene            5320..5961
                     /locus_tag="VC122_001508"
     CDS             5320..5961
                     /locus_tag="VC122_001508"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002991395.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CoA pyrophosphatase"
                     /protein_id="HEP5641425.1"
                     /translation="MKDLLKQYQAKPLGEEKRYAVFLPLILVNDDWHVLYEVRSQHIS
                     QPGEVSFPGGQVENQETLQEAAIRETVEELTVDASQIQLWGEIDYLVQSSRTIHCFVG
                     QLVVDDWKSIQPNEEVDKVFIVPLRQLLFTDPVYYRLEATPIETTDFPFDRIRNGKYY
                     QFSQEYRSIPFYENLEETIWGMTAQFTKCLTDILNDESIAKKNPSDIENSKGR"
     gene            complement(6008..6850)
                     /locus_tag="VC122_001509"
     CDS             complement(6008..6850)
                     /locus_tag="VC122_001509"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002991650.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF368 domain-containing protein"
                     /protein_id="HEP5641426.1"
                     /translation="MVSFISRVFKGMIIALGFILPGVSGGVLAAILGIYERMISFLAH
                     MRDNFIENVLFFLPVGIGGILGIALFSFPVEFLLKHYQVSVLWGFAGAIVGTIPSLIK
                     ESTKQSQRDKADWLWLVLTFVISGLGLYFLNDLIGTLPANFLTFILAGALIALGVLVP
                     GLSPSNLLLILGLYGPMLIGFKSLDLLGTFLPIAIGGVLAILAFSKSMDYALQHHHSK
                     VYHFIIGIVLSSTLLILIPNSSSPESISYSHAGILTWLMAFVLFALGIWLGLWMSQLE
                     EKYK"
BASE COUNT         2177 a         1276 c         1352 g         2132 t
ORIGIN      
        1 atcccttcat tttactatgg cttctaaaaa agttatcact aaggctgatg acttgtaaaa
       61 tgatactttc gtaagggtta ttatttaggt actcgtaagc attttgagaa cgaattgtgt
      121 catttcttga ggtgattctt ttttaccttt agcaatccaa ctttgacaga caccaaaaaa
      181 agcatgggct aagtagatac tttggtattc tttttccgtt tgagataact cttcggtact
      241 gaacttgtcc tgtaaatcag tagtgatgag taggcgaact ttattgatga taaaggcctg
      301 tatttcttta gtaccgttag cagacaaaag ggaagagaga agctgttctc tttggagaaa
      361 ttcaaatact tcaaggaagg cttgttcttt attttgatat tttttctcaa aaatatattc
      421 taatttgtga aaaaaggttt gctgataata gtcaatcatt tcatatttat ctttatagtg
      481 tgtgtaaaaa ctagagcgac taattccagc tcttttagat aatttaactg tggtaatatc
      541 gtcaaaactt tcggttttta gtaacataac cattgctttt aaaattgctt gcttcgtatt
      601 ttctttacgg ttgaccatct accatctctc cttttgaaca cttttagacg taatgtccaa
      661 aagtgaattt ttttctcttg taaatttaaa tttaaattgt ataatatatt ttatcataat
      721 ttttgaacag tatgtccatt ttaggagaaa atatgttaga agaattgaaa acacttatta
      781 aaaatccaaa attaatgatt acaatgattg gtgtggccct agtgcctgcc ttatataatt
      841 tatcctttct aggctcaatg tgggatcctt atggtcgggt caatgacctt cccattgctg
      901 ttgttaatca tgataagcct gcaaagagag ctgataagtc attgacaatt gggaatgata
      961 tggtggacaa gatgtctaaa agtaaagatt tagagtatca ttttgtctca gctaagcaag
     1021 ctcaagaggg acttaaagaa ggtgattatt atatggttat tactttaccc gaagatcttt
     1081 ctcaacgggc agcaacctta ttaaatcccg aaccccaaaa attaactatc cgttaccaaa
     1141 cgagtaaagg acatggaatg gtcgctgcta agatggggga aacagcgatg gctaagctga
     1201 aagagtctgt ttcgcaaaac attacgaaga cttatacctc agcagttttt agcagtatga
     1261 cagacctcca atcaggatta aaagaagcct cagctggaag tcaagcactt gcttcaggag
     1321 cgaagaccgc tcaggcgggg agtcaaacac tttcgacgaa cttagcagcc ttaacgggtg
     1381 ctagccaaca gtttcaacaa ggtactggtc gattgacatc aggtttgact acctatacag
     1441 atggtgtcaa ccaagtcaag aatgggttag gaacattatc aacggacatc cccaattatc
     1501 tgaacggggt ttctaggtta tcccagggag cttctcagct taatcagggt ctttcacagt
     1561 tgacacaagc aacaacactt tctgacgaga aagctaaggg aattcaatcc ttaattgtag
     1621 gactaccagt cctaaatcaa ggcattcagc aactaaatac agagctatca acattgcaac
     1681 cccctaacct taatgctgat gagttaggta atagcttagg agctatcgct caagctgcca
     1741 aacaagttat tgctgaagag actgccgctc agaatgaaca actatcggct ctccaagcca
     1801 ctagtgttta tcaatcatta actgctgaac aacaaggaga gttagctgcg gccctcagtc
     1861 aatctgataa aagtcaaacc gtatctgcag cccaaactat tttaagttct gttcaaacac
     1921 tgtcaacagg tttacaaact ctgtcccaag gaaatagttt agctcaatta gaccagctta
     1981 aagcagctgt ttcacagatt gcgagtcagt ctaaccaggc tttacctgga gcgagttctg
     2041 cattatctga gttgtcaatg gggttatcaa aggtaaatgg tagcttaaat caacaagttc
     2101 taccaggaag taatcaattg acaacaggat tagcacaatt aaacaggtat aatactgtca
     2161 ttggttctgg ggtaataaaa ctctcagaag gtgccaatgc cttgtcatcc aagtccggag
     2221 aattactaga tggtagccat caattatcag agggtgctac taaactagct gatagtagtt
     2281 ctcaattgag tcagggtggt catcaattaa cgagcggatt gactgaatta tcaacaggat
     2341 tatcaacctt aaatggttcc ttagccaaag cctctcagca gttatcgctt gtttctgtga
     2401 ctgataaaaa tgctaaagct gtcgcaaaac ctcttgtgtt aaatgagaaa gacaaagatg
     2461 gtgttaagac gaatgggatc gggatggcac cttatatgat tgctgtttct ctaatggttg
     2521 tggccctttc aaccaacgtc atttttgcta attctttatc tggtcgtccg gtcaaagata
     2581 aatgggattg ggctaaacaa aaatttgtta ttaatggttt tatttcgact atgggatcca
     2641 ttgttctcta cttagctatt caattattag ggtttgaagc ccgttatggt atggaaacct
     2701 taggatttat tatgctaagt ggttggacgt ttatggctct tgtcacagct ttggtcggtt
     2761 gggatgatcg atatggctct tttgcttctt tggtcatgtt attacttcag gttggctctt
     2821 caggtggctc ttaccccatt gagttaagtg gagcattctt ccaaaagtta catcctttct
     2881 taccaatgac ttatgtggta tctggtttac gacaaaccat ttcattatca ggtcatattg
     2941 gagtagaagt gaaagtctta actggtttct tactggcatt tatggtatta gcactactca
     3001 tttatcgtcc caagaaaaca gtctaaataa aataacctca aaaaaaaaca gcatctaagg
     3061 cagttacact agatgctgtt ttttgtctta ttttgacaat aacttgacaa tttctgtcaa
     3121 gttttttatt gtcttgttaa gattatttga tagtaatgtc tcccttattg agattaaccg
     3181 ttaaatttcc agtagctgct ttattctcag ccacataaga aataacatct gaagttgatt
     3241 ccttaccaat cttagctggt gtcagctgtg atccaagtaa gattttacca tcttcggttt
     3301 ttgtcttata ggcggtcgtc tttggatcgg tcaaagttat gtcaacagca acattaccac
     3361 cactaatagt attagtgcct ttaagggtaa gagcatgagc agtgaatata gcaccattat
     3421 cattggtttc ctcgttttct tgctcactag taacagtgaa agtcacattt tcaaggcttg
     3481 tttcttcagt ttcaacccca ccaggagata gagattggat ggtgacagat ttaagattag
     3541 tattttttgt ggttaattgt ccatgcccta agctaatcgt cccattttct aaactagaat
     3601 tcgtaaggct tgacaacgaa tagtcggctt tcaactcaag attggctaac aagctatcag
     3661 tagcgttaaa tgttccaaaa accttccctt tctttattct agtatttaga agtgtcagac
     3721 caccactgaa ctcaaggtct tgaaccgtta aatccgatat agtggcttga ggcatgcttg
     3781 agcctttgag ctctttaaga ctggtacctt ttggaagcgt tatggtagtg gttcggtagt
     3841 tgtggttgcg gtcactttta tttaatccaa acccgatcag ttcaatacct ccggtgatga
     3901 tgacatctct gggtttttca gaaatatgaa gtgtctcgtt ctctatttta atttctaaag
     3961 gatcaataaa tttagaatgt gtgtaatagg taacggttgg ttttttgaca ggccctgttt
     4021 ctactgtaat agttgaaaaa ttggtatcaa tatcaagagc tgttacgtga tcaaatgttt
     4081 ggataacctt tttaggggct gtttggtgtt taatgtcttg aagcccgcca gttgcataac
     4141 cagctcctaa aagaatggct cctaaaacaa tacacacgac actggtcata aaacatttgc
     4201 gtgagatatt tttcatgctt gagctccttt cttgtgacta atccatttaa taaaggcaac
     4261 gaaggcacgt cctgaaaaac gcaggagtgc agtggcaata atccaaatga gaagaccacc
     4321 accaaaacac aacaaggcgc tgccgattcc catagcttgt gcaggaatag atgagccaat
     4381 caaggtcaag ctttcaaaca gggtcactcc tgcgacaaga atacctgcaa gcccaaaaat
     4441 gtaggcggca aagatagctg tgagcccgag cattaggcag gcaataataa agagaatcaa
     4501 cgccagcaat acgggtaaag caatcggagc agcaaagagg gacaaaatgg ttaatccaat
     4561 aattgttgcc cgatttttgg gagttttctc gggagttgcc aagtgttttc ctagaacatt
     4621 tgaaatgatt tcactggcag cttccttagg atttcctaaa tcgtcgataa cctggctttc
     4681 tttgtcagga ccagcttcat caaaatattc aatataatat tccaacgctt catggtagtc
     4741 ttctctaggt agtttgcgca agtatttatc tagttctgct agatactcag tccttgtcat
     4801 gtcttaagct cccttcaata atcccgtcta aggcaaattt atagctctgc cattcttttt
     4861 ttagaaaaat cagctgtgct ctccccgaag aagtgacagc ataatattta cgctttcgtc
     4921 cctgatgttc ttgactataa gttgtcaaaa aacctgcctt ctccaatttt ttaagaatag
     4981 gatagagggt ggattctttg atattagcca ctagtttaat ggtttgacta atatcgtaac
     5041 cgtaagaatc gtttttttcg acaatggcta aaatcagaaa ttcaatcaag gtagccgata
     5101 ctggaaaata cattttaggc tcctttcata tataaaaatt ttatatataa ttattttaca
     5161 tatttagtat aagcttagtt ttttgatatg tcaatatttt atatataaaa aaataacata
     5221 tatatcaaaa atcactaact aggagtaata aattgaccaa aaagctagga ttggttatga
     5281 tgaagataag attaacggaa agagaaatta gggaaggcta tgaaggactt attaaaacag
     5341 tatcaggcaa aaccactagg agaggagaag cggtacgccg tatttttacc tcttattctt
     5401 gtcaatgatg actggcatgt cttatatgaa gtcagaagtc agcatatttc acaaccgggg
     5461 gaagtttctt tcccaggggg acaagtagaa aatcaggaga cacttcaaga agctgctatc
     5521 cgagaaacag ttgaggaatt gactgttgat gcgtcccaga tccagctatg gggtgaaatt
     5581 gattatttag tacagtcttc tagaactatt cattgtttcg ttggtcaatt agtcgttgat
     5641 gactggaaaa gcatacagcc taacgaagaa gttgacaagg tctttatagt acctttacgt
     5701 cagctacttt tcactgatcc tgtctattat cgtttagaag ccactcctat tgagacaacg
     5761 gattttcctt ttgaccgtat tcgtaatggg aaatactacc aatttagtca agagtaccgt
     5821 agtattccgt tttatgaaaa tctagaagaa accatatggg gaatgactgc ccagtttact
     5881 aagtgtttaa ccgatattct aaatgatgaa tcaattgcaa agaaaaaccc ctcagatatt
     5941 gaaaattcta aggggagata gagcactagt ttgttggcgt caactttttg tcaacaaact
     6001 gtaaaggtta tttgtatttt tcttctaatt gactcatcca aagtccgagc caaataccaa
     6061 gagcaaataa cacaaaggcc ataagccagg ttaagatacc tgcatgactg taggaaatgg
     6121 attcgggact actactattt ggaataagaa ttaatagcgt gctggataat acaataccaa
     6181 tgataaagtg gtaaactttt gaatgatggt gctgtaaagc ataatccata cttttagaaa
     6241 aagccaagat ggctaggact cctccgatag caataggtaa gaaagtccct aataaatcca
     6301 aagatttgaa ccctattaac ataggaccat agagtcctaa gataagtaag agatttgacg
     6361 ggcttagtcc agggaccagt actcctaaag caattaaggc acctgctaaa ataaaggtta
     6421 aaaagttagc tggtaaggta ccgattaagt catttaagaa gtaaagacct agtcctgaaa
     6481 taacaaaggt aaggactagc catagccagt cagctttgtc tctttgagac tgcttggttg
     6541 actccttaat cagactagga atagtgccga caatagcgcc agcaaatccc cataagacgc
     6601 taacttgata gtgcttaagc aaaaactcaa cggggaaaga aaagagagca atacctaaaa
     6661 taccaccaat tcctactggt aagaaaaata agacattttc aataaagtta tccctcatat
     6721 gagctaaaaa ggaaatcatt cgctcataga ttcctaaaat ggctgcgaga acaccaccgg
     6781 atacaccagg taaaataaaa cctagtgcaa taatcatgcc tttaaaaact cttgaaataa
     6841 atgaaaccat tgttgcctcc tgaaaagaat gattttattg tatcataaat ttaaagaatt
     6901 gctgacacaa agctgcatat ccctactaaa ataattg
//
LOCUS       DATXIG010000069         6825 bp    DNA     linear   BCT 28-DEC-2023
DEFINITION  TPA_asm: Streptococcus pyogenes strain MGAS11113
            SAMN08555799-rid19682583.denovo.069, whole genome shotgun sequence.
ACCESSION   DATXIG010000069 DATXIG010000000
VERSION     DATXIG010000069.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN08555799
            Sequence Read Archive: SRR6740495
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pyogenes
  ORGANISM  Streptococcus pyogenes
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 6825)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6825)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-OCT-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-OCT-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001918058.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001918058.2
            Genome Coverage       :: 368x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/12/2023 11:23:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 1,806
            CDSs (total)                      :: 1,769
            Genes (coding)                    :: 1,722
            CDSs (with protein)               :: 1,722
            Genes (RNA)                       :: 37
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1, 1 (5S, 23S)
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 47
            CDSs (without protein)            :: 47
            Pseudo Genes (ambiguous residues) :: 0 of 47
            Pseudo Genes (frameshifted)       :: 30 of 47
            Pseudo Genes (incomplete)         :: 20 of 47
            Pseudo Genes (internal stop)      :: 9 of 47
            Pseudo Genes (multiple problems)  :: 11 of 47
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6825
                     /organism="Streptococcus pyogenes"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN08555799-rid19682583.denovo.069"
                     /strain="MGAS11113"
                     /serotype="Emm28"
                     /isolation_source="normally sterile site"
                     /host="Homo sapiens"
                     /db_xref="taxon:1314"
                     /country="Finland"
                     /collection_date="2002"
                     /collected_by="National Public Health Laboratory"
     gene            107..838
                     /gene="aphA"
                     /locus_tag="VC122_001510"
     CDS             107..838
                     /gene="aphA"
                     /locus_tag="VC122_001510"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002984720.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acid phosphatase AphA"
                     /protein_id="HEP5641427.1"
                     /translation="MKKEFTSILFTVSFCGIIALPVEASGPKVPYTQEGITAISNQAT
                     VKLISIADIASSLEGQKPITVSFDIDDTLLFTSQYFQYGKEYITPGSFDFLHKQKFWD
                     LVAKRGDQDSIPKEYAKQLIAMHQKRGDKIVFITGRTRGSMYKKGEIDKTAKSLAKDF
                     KLDKPIAINYTGDKAVKPYQYDKTYYIKKNGSQIHYGDSDEDINAAKEAGARPIRILR
                     APNSTNLPLPKAGGYGEEVLENSAY"
     gene            1016..2548
                     /locus_tag="VC122_001511"
     CDS             1016..2548
                     /locus_tag="VC122_001511"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014635458.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ClC family H(+)/Cl(-) exchange transporter"
                     /protein_id="HEP5641428.1"
                     /translation="MENHKNEFTFSNKSIIAYVWRGVMVGIIAGVIVSLFRLLIEVTA
                     DWVIEWYRYAHINSLLLLPILSVSLLAVLFVGFLVKSDSDIKGSGIPHVEGELKGLMS
                     PDWWSVLWKKFLGGIMAISMGFMLGREGPSIQLGAMSAKGLAKFLKSSRLEKRVLIAS
                     GAAAGLSAAFNAPIAGLLFVVEEIYHHFSRLIWITALVASLVANFISLNIFGLKPVLA
                     MSEAMPFLGLNQYWLLLLLGLFLGCLGYLYEIVILNFNKLYVILGNWLHLPAYFYGII
                     MVFLILPIGYYLPQLLGGGHGLILSLSNQQLPLMTIFFYFIIRFIVSMFSYGSGLPGG
                     IFLPILTLGALAGLLFGQIASQLGLLNQSFLSLFLILGMAGYFAAISKAPLTGMILVT
                     EMVGDLKPLMAIAVVTFVSYLVMDLLNGQPIYEAMLDKMAMKHPTNLVEPTLIELTVG
                     DKIAGKYVKELKLPENVLITTQIHHQKSQVVSGNTRLLSGATIFLVVNEADTGFVREV
                     LM"
     gene            2711..3325
                     /locus_tag="VC122_001512"
     CDS             2711..3325
                     /locus_tag="VC122_001512"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002984715.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipase"
                     /protein_id="HEP5641429.1"
                     /translation="MLEIVSEELRHYQEQKLIEYRNKNQLAPKGGIVFAGDSLIEFFP
                     LKKAFGSCLPIINRGIAGIDSQWLLRHFSVQITDLEPKHIFLLIGCNDIGLGYDKCHI
                     VKTIVELISQIRSHCVYSQIYLLSLLPVSNNPRYQKTVKIRTNAMIDAINKDLAMIPT
                     IEFINLNTCLKDEKGGLSDENTLDGLHLNFPAYAKLAEIIQAYI"
     gene            3484..4632
                     /locus_tag="VC122_001513"
     CDS             3484..4632
                     /locus_tag="VC122_001513"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002989837.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AI-2E family transporter"
                     /protein_id="HEP5641430.1"
                     /translation="MIIKGVTMKFEKKQVFYLVLTFILCYGILANWRNGTAIVTTIYK
                     TSLPFFYGAAGAYIVNIVMSAYEKVYVYIFKDWSHVLKVKRGICLLLAYLTFFILITW
                     IISIVIPDLITSISTLTKFDTITIQEVVNNLEHNKLLARTIQYIGGDGKLTETIANYS
                     QQLLKQFLTVLTNILTSVTVIASAIINLFISFVFSLYVLASKEDLCRQGNTLVDTYTG
                     KYAKRIHYLLELLHQRFHGFFVSQTLEAMILGSLTASGMFILRLPFAGTIGVLVAFTA
                     LIPVIGASIGAAIGFILIMTQSMSQAIIFIIFLIILQQIEGNFIYPKVVGGSIGLPAM
                     WVLMAITIGASLKGIVGMIIAVPLAATLYQVIKDNIQKRQAIQKKQVS"
     gene            4681..5361
                     /gene="radC"
                     /locus_tag="VC122_001514"
     CDS             4681..5361
                     /gene="radC"
                     /locus_tag="VC122_001514"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002984709.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein RadC"
                     /protein_id="HEP5641431.1"
                     /translation="MYSIKCDDNKAMPRERLMRLGAESLSNQELLAILLRTGNKEKHV
                     LELSSYLLSHLDSLADFKKMSLQELQHLAGIGKVKAIEIKAMIELVSRILATDKTLTD
                     SVLTSVQVAEKMMAALGDKKQEHLVVLYLDNQNRIFEEKTIFIGTVRRSLAEPREILY
                     YACKNMATSLIVIHNHPSGNIEPSSNDYCFTEKIKRSCEDLGIICLDHIIVSYKDYYS
                     FREKSTLF"
     gene            complement(5363..6058)
                     /locus_tag="VC122_001515"
     CDS             complement(5363..6058)
                     /locus_tag="VC122_001515"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002984707.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gamma-glutamyl-gamma-aminobutyrate hydrolase
                     family protein"
                     /protein_id="HEP5641432.1"
                     /translation="MTKPIIGITANQRLNMALDNLPWSYAPTGFVQAVTQSGGLPLLL
                     PIGDEAAAKTYVSMVDKIILIGGQNVDPKYYQEEKAAFDDDFSPERDTFELAIIKEAI
                     TLKKPILGICRGTQLMNVALGGNLNQHIDSHWQEAPSDFLSHEMIIEPDSILYPIYGH
                     KTLINSFHRQSLKTIAKDLKVIARDPRDGTIEAVISTNDAIPFLGVQWHPELLQGVRD
                     EDLQLFRLFVNDF"
     gene            complement(6068..6712)
                     /locus_tag="VC122_001516"
     CDS             complement(6068..6712)
                     /locus_tag="VC122_001516"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003054034.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="redox-sensing transcriptional repressor Rex"
                     /protein_id="HEP5641433.1"
                     /translation="MVIDKSIPKATAKRLSLYYRIFKRFHADQVEKASSKQIADAMGI
                     DSATVRRDFSYFGELGRRGFGYDVTKLMNFFADLLNDHSTTNVILVGCGNIGRALLHY
                     RFHDRNKMQIAMGFDTDDNALVGTKTADNIPVHGISSVKERIANTDIETAILTVPSIH
                     AQEVTDQLIEAGIKGILSFAPVHLQVPKGVIVQSVDLTSELQTLLYFMNQNHLD"
BASE COUNT         2146 a         1064 c         1312 g         2303 t
ORIGIN      
        1 ataacttcat ccttattcag aacttaaagg gcactattat aagaatttga aaccgcttta
       61 cttaaaccgt gtatttttgt acaataatga ttaaggaggt ttttagatga aaaaagaatt
      121 taccagtatc ctgtttacag ttagtttttg tggaatcata gcattgcctg tcgaagctag
      181 tggccctaaa gttccttata cacaagaagg tataacggcc atttcaaatc aggcaacagt
      241 aaaattgatt tctattgctg atatcgcgag cagtttagaa ggacaaaaac ctataacggt
      301 tagttttgat atcgatgata ctttgttatt tactagccaa tattttcagt acggcaaaga
      361 atatataact ccaggctcat ttgatttttt gcataagcaa aaattttggg acttagtcgc
      421 taaacgggga gatcaggatt ctattccaaa agagtatgca aagcaactaa tcgcaatgca
      481 tcaaaaacga ggagataaaa ttgtctttat tactggccga actcgaggat cgatgtataa
      541 aaaaggtgag attgataaaa cagctaagtc actggctaaa gattttaagt tagacaagcc
      601 aatagctatc aattatacag gagataaagc tgtgaagccg tatcagtatg ataaaacata
      661 ttatataaag aaaaacggct cacaaattca ttacggagat agtgatgaag atataaatgc
      721 tgccaaagag gcaggtgcaa gacctattcg cattttaaga gctcctaatt caaccaatct
      781 tcctttgcca aaagctggag gatacggaga agaggtttta gaaaattcgg cttattagtc
      841 aaatatttgt gtaagaaagg aagtaacagt aatttctttc atcaccccac tgagtaacca
      901 atagcccagt gggttttttg ggttacagaa atcagttcta aaaaagaatc actggtaact
      961 taaggttgtc tttgatacaa tggtaacagt gtatttaaaa gaaagagagg ataacatgga
     1021 aaaccataaa aatgaattca ccttttccaa caaatcaatt attgcctatg tttggcgtgg
     1081 tgttatggtt ggtattattg ctggtgttat tgttagttta ttccggttgt taattgaagt
     1141 gacagctgac tgggtgattg aatggtaccg atacgctcat atcaattcgc tgttgctact
     1201 tcctatacta agtgtcagtt tgttagctgt tctttttgta ggttttttag taaagtctga
     1261 ttctgatata aaaggatcag ggatccccca tgtcgaagga gaactaaagg ggctaatgtc
     1321 cccagattgg tggagtgtcc tttggaaaaa atttttagga ggaatcatgg ctatttctat
     1381 gggatttatg ctcggtcgtg agggtccttc gattcagtta ggtgctatgt cagccaaagg
     1441 attagctaag tttttaaaat cgagtcgttt ggaaaagcga gtgctaattg ccagcggagc
     1501 agcagcaggg ctatcagcag cttttaatgc tccaatagca ggcctcttgt ttgttgttga
     1561 agaaatttac catcattttt cgcgtttaat ttggattaca gcgctagtgg ctagcttagt
     1621 ggctaatttt atttctctga atatttttgg attgaaacca gtcttagcaa tgtcagaggc
     1681 gatgcctttt ttagggctaa atcaatattg gttgttgctt ttacttggat tatttctagg
     1741 ctgcttaggc tatttatatg agatagttat ccttaatttt aataaacttt atgttatttt
     1801 agggaattgg ttacatctgc cagcttattt ttacggtatt attatggttt ttctaatttt
     1861 accgattggc tattatttac cacagttact tggaggaggg catggtttaa tactttcttt
     1921 atccaatcaa caattgcctt tgatgactat cttcttttat tttattattc gatttattgt
     1981 tagtatgttt agttatggaa gtggcttacc tggaggtatt tttttaccca ttttaacttt
     2041 aggtgctttg gcaggcctat tatttgggca aatagcttct caattaggtt tattgaacca
     2101 atcttttctt tctctttttt tgatattagg gatggcaggg tattttgcgg caatttcaaa
     2161 agcaccactg acaggcatga ttttggtaac tgaaatggta ggtgatttga aacccttaat
     2221 ggccatagct gtagttactt ttgtctccta tttagtgatg gatttgttaa atggacagcc
     2281 aatttacgaa gctatgctag ataagatggc aatgaagcat ccaactaatc ttgttgaacc
     2341 aacattaatt gaattgactg ttggtgataa gattgcaggg aaatatgtta aagagttaaa
     2401 gcttccagaa aatgtcttaa ttacaaccca aattcaccat caaaaatctc aagttgtgtc
     2461 aggtaacacg cgccttttgt caggagcaac tatctttttg gtcgttaatg aagctgatac
     2521 aggttttgtc cgtgaggtat tgatgtaggt attaatttat cacaattcgg aaagttcgag
     2581 cttgtctttt agactaagtc ttcgaacgtt ttactgctag agtttcatct gaaccaaagt
     2641 gaaacgcttt tgatgaagtc aagtaggctt tttgttatac taacaataaa gataagctaa
     2701 ggaggctgag atgttagaaa ttgtttcaga agagctacgt cactatcaag aacaaaaatt
     2761 gattgagtac cgtaacaaaa atcagttagc cccaaaagga ggcattgtat ttgcaggtga
     2821 ttctctcatt gaattttttc ctttaaaaaa agcttttggt tcatgtttgc ctattattaa
     2881 tcgaggtatt gctgggatag atagtcagtg gttattgcgc catttttctg tccaaattac
     2941 tgatttagag cctaaacata tttttttatt aattggttgt aatgatatcg ggcttggata
     3001 tgataaatgt catattgtca agactattgt agagcttatt agccagattc gtagtcattg
     3061 tgtctacagt cagatttatc tcttatcttt attaccagtt tccaacaatc cccgatatca
     3121 gaagactgtc aaaataagaa caaatgctat gattgatgct attaataaag atttagcaat
     3181 gattccaact atagaattta ttaatcttaa tacttgtctt aaagacgaaa aaggtggttt
     3241 atcagatgag aatactctag atggtttaca tcttaatttt ccagcttatg ctaaactagc
     3301 tgaaattatt caagcttata tctagcaaat aaatgtacta atagcgatga ggcaagaagt
     3361 cagatagcag atggttcatc aatcaattaa gaaaaaagtt atcaagatac cattaaaaat
     3421 agttgctcaa agttctttca aatcaaaaga ttatgatatc gctatgttga gttttagtgt
     3481 ataatgataa ttaaaggagt taccatgaaa tttgaaaaaa aacaagtctt ctatttagtc
     3541 cttacgttta tcttatgcta tgggatccta gcaaactggc gaaatggaac ggctatcgtc
     3601 acgactattt acaaaacaag ccttcctttt ttttatggag cagcaggtgc ctatattgtt
     3661 aatattgtaa tgagcgctta cgaaaaggta tatgtttata tctttaaaga ctggtcacat
     3721 gtcttaaaag taaaacgggg tatttgttta ctgttagcgt atttgacatt ttttatttta
     3781 attacttgga ttatttccat tgtcattcca gacttaatta caagtattag tactctaaca
     3841 aaatttgata ctataacgat acaagaagtt gttaacaatc ttgagcacaa taaactgtta
     3901 gcacgtacta ttcagtatat tggtggcgat ggcaaactta cagagacgat tgctaactat
     3961 agtcaacagt tattaaagca atttcttact gttttgacaa atattttaac atctgtcacg
     4021 gttattgctt ctgcaattat taatcttttt attagttttg ttttttcgtt atacgtttta
     4081 gcaagtaaag aagatctttg tcgtcaggga aatactttag tagatactta tactggtaag
     4141 tacgctaaac gcattcatta cttgttagag ttgttgcatc agcgttttca cggatttttt
     4201 gttagtcaga ccttagaagc tatgatttta ggttcgctga ctgctagtgg catgtttata
     4261 ttaagattac catttgcagg gactattggc gttttagtag cttttacagc attgattcca
     4321 gttataggtg cttcaattgg agcagctatt ggatttattt taattatgac tcagtccatg
     4381 tcacaggcca ttatttttat cattttttta attattttgc agcagattga aggtaatttt
     4441 atttatccga aagtagttgg tggatcgatt ggattaccgg ctatgtgggt attaatggca
     4501 attacaatag gcgcttcttt aaagggaata gttggtatga ttattgcagt tcctttagca
     4561 gcgacacttt atcaagtgat taaagataat attcaaaaaa gacaagctat tcaaaaaaaa
     4621 caagtttcct aaaaggaact tgtttttttg tcgaataaaa agatggaggt aatatgataa
     4681 atgtattcta taaaatgtga tgataataaa gccatgccaa gagaacgttt gatgcgacta
     4741 ggagcagagt ctctaagtaa tcaagaatta ttagcaattt tattacgaac aggtaataaa
     4801 gaaaagcatg tcttagagct gtcatcctat cttttatcgc atttagacag tctggcagat
     4861 tttaaaaaga tgtctttgca agaattacaa catttggcag gtataggaaa agttaaagcg
     4921 attgaaatta aagctatgat tgagttggtt tcccgaattt tagcgaccga taagacatta
     4981 actgatagcg tattaaccag tgttcaggtc gctgaaaaaa tgatggcagc tttaggagat
     5041 aaaaaacaag agcatttagt cgtattgtat ttagataatc aaaatcgtat ttttgaagaa
     5101 aaaactattt ttattgggac tgtccgacgt tcacttgcag aaccaagaga aattttatac
     5161 tatgcctgta aaaatatggc gactagtctc attgttattc ataatcatcc ttcaggaaat
     5221 attgaaccta gttctaacga ttattgcttt actgaaaaaa taaaacgatc atgtgaagat
     5281 ttaggcatta tctgtctaga tcacattatc gttagctata aagattatta tagttttcga
     5341 gaaaaatcaa ccctttttta aatcagaagt cattgacgaa taagcggaac aattgtagat
     5401 cttcatctcg aacaccttgg agaagttcag gatgccactg aacacctaaa aaaggaatgg
     5461 catcgttggt tgatatgact gcttcgatgg taccatcacg tggatctcta gcgattactt
     5521 ttaaatcctt agcaatagtt tttaaacttt ggcgatgaaa agaattaatt aatgtcttat
     5581 gaccgtaaat aggatataag atagagtctg gctcaataat catttcgtgt gataagaaat
     5641 cagaaggcgc ttcttgccaa tgtgagtcaa tgtgttggtt aagatttcct cctaaggcaa
     5701 cattcattaa ctgtgtacca cgacaaatcc ctaagattgg tttttttaat gttatagcct
     5761 ccttgatgat agctagctca aatgtgtctc gctctggtga aaaatcatca tcaaaagcag
     5821 ccttctcttc ttgataatat tttgggtcaa cgttttgccc accaattaaa atgattttat
     5881 caaccataga tacataagtt ttagcggcgg cttcatcacc aatgggtaaa agaagcggca
     5941 gccccccaga ttgagtgact gcctgtacaa atccagtagg tgcatatgac catgggagat
     6001 tatccaaagc catatttagt ctttgatttg cagttattcc aataatcggt ttggtcatta
     6061 tacctcctta atctagatga ttttgattca taaaatagag tagtgtctgg agctcactgg
     6121 tcaaatcgac agattgaaca ataactcctt taggaacttg taggtgaaca ggagcaaaac
     6181 tcaagattcc tttgattcct gcttcaataa gttgatcagt aacttcttga gcatgaatgc
     6241 tgggaaccgt taagatagcc gtttcaatgt cagtgtttgc aatccgttct ttgacggatg
     6301 agataccatg gactggaata ttatcagctg ttttagtacc aacaagagcg ttatcatcgg
     6361 tatcaaaacc catggcaatt tgcatcttat tacgatcatg gaagcggtaa tgtaatagag
     6421 cacgccctat attaccgcat ccaactaaga taacgtttgt tgtagagtga tcattaagca
     6481 agtcagcaaa aaaattcatc aatttggtaa catcatagcc aaacccacga cgccccaatt
     6541 ctccaaaata agaaaagtca cggcgtacag tagcagagtc aattcccatt gcatctgcaa
     6601 tttgtttaga actggctttt tcaacttggt cagcatggaa tcgtttaaaa atacggtaat
     6661 aaagtgataa gcgctttgca gtcgcttttg gaatagattt gtcaataacc actattttac
     6721 tccttgtaga tttctcgtta aatatgtctg tctttattgt aacaaaaaga ttgtgaaaaa
     6781 tgcaacattt tgattgtttt tgtatgagtt aaaaaagcta aaatt
//
LOCUS       DATXIG010000070         6783 bp    DNA     linear   BCT 28-DEC-2023
DEFINITION  TPA_asm: Streptococcus pyogenes strain MGAS11113
            SAMN08555799-rid19682583.denovo.070, whole genome shotgun sequence.
ACCESSION   DATXIG010000070 DATXIG010000000
VERSION     DATXIG010000070.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN08555799
            Sequence Read Archive: SRR6740495
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pyogenes
  ORGANISM  Streptococcus pyogenes
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 6783)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6783)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-OCT-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-OCT-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001918058.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001918058.2
            Genome Coverage       :: 368x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/12/2023 11:23:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 1,806
            CDSs (total)                      :: 1,769
            Genes (coding)                    :: 1,722
            CDSs (with protein)               :: 1,722
            Genes (RNA)                       :: 37
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1, 1 (5S, 23S)
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 47
            CDSs (without protein)            :: 47
            Pseudo Genes (ambiguous residues) :: 0 of 47
            Pseudo Genes (frameshifted)       :: 30 of 47
            Pseudo Genes (incomplete)         :: 20 of 47
            Pseudo Genes (internal stop)      :: 9 of 47
            Pseudo Genes (multiple problems)  :: 11 of 47
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6783
                     /organism="Streptococcus pyogenes"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN08555799-rid19682583.denovo.070"
                     /strain="MGAS11113"
                     /serotype="Emm28"
                     /isolation_source="normally sterile site"
                     /host="Homo sapiens"
                     /db_xref="taxon:1314"
                     /country="Finland"
                     /collection_date="2002"
                     /collected_by="National Public Health Laboratory"
     gene            <1..994
                     /locus_tag="VC122_001517"
     CDS             <1..994
                     /locus_tag="VC122_001517"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002989251.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="polysaccharide deacetylase family protein"
                     /protein_id="HEP5641434.1"
                     /translation="ILVKPKLQSSHLRSVNTLTISKIVYQKKFFHLAKKSEKVISTYH
                     VTDDLKPFQVRDLVSGHLERIQEEVEKKYPNAGFNSDKYNGLKESNSLLSDGFEVKSG
                     NLIFDKKLTIPLTTLFDVINPDFLANSDRAAYDNYRTYKEQHPKKLVALTFDDGPDPT
                     TTPQVLDILAKYQAKGTFFMIGSKVVNNENLTKRVSDAGHEIANHTWDHPNLTNLSVS
                     EIQHQVNMTNQAIEKACGKKPRYLRPPYGATNATVQQSSGLTQMLWTVDTRDWENHST
                     DGIMTNVKNQLQPGGVVLMHDIHQTTINALPAVMEYLKAEGYECVTVSELYAHQ"
     gene            complement(1104..2189)
                     /locus_tag="VC122_001518"
     CDS             complement(1104..2189)
                     /locus_tag="VC122_001518"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014407659.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase"
                     /protein_id="HEP5641435.1"
                     /translation="MITKFPPQWQEKLDQVAFTHLTPIQEQAFQPIVDGKNFLGISPT
                     GTGKTLAYVFPSLLALTPKKSQQLLILAPNTELAGQIFEVTKDWAQPLGLTAQLFISG
                     TSQKRQIERLKKGPEILIGTPGRIFELIKLKKIKMMSVNTIVLDEYDELLGDSQYDFV
                     QKISHYVPRDHQMVYMSATNKVDQTSLAPNTFCIDLSEQTNDAIQHFYLMVDKRERTD
                     LLRKFANIPHFRALVFFNSLSDLGATEERLQYNGAAAVSLASDINVKFRKTILEKFKS
                     HQLSLLLATDLVARGIDIDNLDYVIHFDVARDKENYTHRAGRTGRMGKSGIVITFVSH
                     PEDLKKLKKFAKVSEISLKNQQLHFIN"
     gene            2287..2913
                     /gene="udk"
                     /locus_tag="VC122_001519"
     CDS             2287..2913
                     /gene="udk"
                     /locus_tag="VC122_001519"
                     /EC_number="2.7.1.48"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012515313.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="uridine kinase"
                     /protein_id="HEP5641436.1"
                     /translation="MLKKPIIIGVTGGSGGGKTSVSRAILDSFPNARIAMIQHDSYYK
                     DQSHMSFEERVKTNYDHPLAFDTDFMIQQLKELLAGRPVDIPIYDYKKHTRSNTTFRQ
                     DPQDVIIVEGILVLEDERLRDLMDIKLFVDTDDDIRIIRRIKRDMMERGRSLESIIDQ
                     YTSVVKPMYHQFIEPSKRYADIVIPEGVSNVVAIDVINSKIASILGEV"
     gene            2993..3256
                     /locus_tag="VC122_001520"
     CDS             2993..3256
                     /locus_tag="VC122_001520"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002984063.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1294 domain-containing protein"
                     /protein_id="HEP5641437.1"
                     /translation="MLLIWLGLLFVWNELVFVIYAFDKRKAIKKKRRISERNLLVITV
                     LFGGFGALLAAKKYHHKTRKWYFVITWYTSILLTLLVTYIFMK"
     gene            complement(3309..4118)
                     /locus_tag="VC122_001521"
     CDS             complement(3309..4118)
                     /locus_tag="VC122_001521"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011017928.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PrsW family intramembrane metalloprotease"
                     /protein_id="HEP5641438.1"
                     /translation="MKQITQQKWLRYGLFIALVLNGIELELLGLTANNLSFKEAFALI
                     LTISLLGIYLIPFAAAIFYLSKKFHMNLNVIIVSCLSGLYISGFLASCGNHLVGQFWS
                     YIIPSKDALKLWGDALTAPIVEEPIKASSAILVITLFPRLTLKEKLVVALLSGMGFQL
                     AEDIRYLIQAKSIDSLVPTAIERISTAVTSHWVHTAIFTIGAYLLLKGSNLFSKQQQI
                     FWLLSPLVLHFIWNSPLTSIPGMTVLLGTLILLIFGDLFQKINTLDDDVLF"
     gene            4263..4760
                     /locus_tag="VC122_001522"
     CDS             4263..4760
                     /locus_tag="VC122_001522"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012678242.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GAF domain-containing protein"
                     /protein_id="HEP5641439.1"
                     /translation="MNKSKKIEQYQLMIAQAKELFANESNALANLSNASALLNMTLPN
                     SVFAGFYLFDGQELILGPFQGRVSCVHIKLGKGVCGESAQSRRTIIINDVKQHANYIS
                     CDAAAMSEIVVPMVKEGHLIGVLDLDSSLVADYDEVDQEYLEAFVDLFLEKTTFTFNM
                     FGVKN"
     gene            4760..6445
                     /gene="dnaX"
                     /locus_tag="VC122_001523"
     CDS             4760..6445
                     /gene="dnaX"
                     /locus_tag="VC122_001523"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002984073.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit gamma/tau"
                     /protein_id="HEP5641440.1"
                     /translation="MYQALYRKYRSQTFDEMVGQSVISTTLKQAVESGKISHAYLFSG
                     PRGTGKTSAAKIFAKAMNCPNQVDGEPCNQCDICRDITNGSLEDVIEIDAASNNGVDE
                     IRDIRDKSTYAPSRATYKVYIIDEVHMLSTGAFNALLKTLEEPTENVVFILATTELHK
                     IPATILSRVQRFEFKAIKQKAIREHLAWVLDKEGIAYEVDALNLIARRAEGGMRDALS
                     ILDQALSLSPDNQVTIAIAEEITGSISMLALGDYVRYVSQEQATQALAALETIYDSGK
                     SMSRFATDLLTYLRDLLVVKAGGDNQRQSAVFDTNLSLSIDRIFQMITVVTSHLPEIK
                     KGTHPRIYAEMMTIQLAKEEGVALPSGLSSDVTTEVEGLKRELNQLKQALAQLQASPS
                     PSHAPVKPAQAKPKLTTYKIDRVKILKIMEETVQDSQQSRQYLDALKTAWNEILDSIS
                     AQDRALLMGSEPVLANSENAILAFEAAFNAEQAMKRTDLNAIFGNIMSKAAGFSPNIL
                     AVPKADFHHIRSEFALQLKTQKASDPTEETIEETLEIPEGFDFLVDKINAVED"
BASE COUNT         2195 a         1139 c         1381 g         2068 t
ORIGIN      
        1 gattttagta aagcctaaac tgcaatcttc tcacctaaga tcagttaaca ctttgactat
       61 ttctaaaata gtttatcaga aaaaattttt tcatttggct aaaaaatcag aaaaagttat
      121 aagtacatat cacgttacag acgacttgaa accgtttcag gtaagggatc tagtatcagg
      181 acatttagaa agaatacaag aagaagttga aaaaaaatat ccaaatgctg gttttaatag
      241 cgataagtac aatggcttaa aagaatctaa ttctttatta agcgatggct ttgaggtaaa
      301 atcgggaaac cttatttttg ataaaaagct aacgatacct ttgacgacat tatttgatgt
      361 tattaatcca gattttttag caaatagcga tagagctgcg tatgataatt ataggaccta
      421 caaagaacag catcccaaaa aactagttgc attaacgttt gatgatggtc cagatccgac
      481 gacgactcct caagttttag atattttggc aaaataccag gctaagggaa ctttctttat
      541 gataggttca aaagttgtga ataatgaaaa ccttactaaa cgtgttagcg acgctggcca
      601 tgaaattgct aatcatactt gggatcatcc caatctgact aatctttcag tcagcgagat
      661 tcaacatcaa gttaatatga cgaaccaggc tattgaaaaa gcttgtggca agaaacctcg
      721 ctatttacgc cctccatacg gagctacaaa tgcaactgtt cagcaatcct caggattaac
      781 acaaatgctt tggacggttg atacaagaga ttgggaaaat catagcactg atggcattat
      841 gactaatgtc aaaaaccaat tgcaaccggg aggagttgtt ttaatgcatg atattcatca
      901 aacaactatc aatgctttgc cggcagttat ggaatatcta aaagcagaag gatacgaatg
      961 tgtgactgta tcagaactct atgcgcatca gtaagtaata tcgttatgtt tctttagaat
     1021 tgatatacta aacaatccaa ttactttgaa gtaattggat tgttgttttt agtagccctt
     1081 gattaagttt tgttttttaa caactagttg atgaaatgaa gttgttgatt tttgagggag
     1141 atttcagaga ctttagcaaa ttttttcaat tttttgaggt cttcagggtg gctaacaaag
     1201 gtaataacga taccagattt tcccatacgg ccggttcgtc cagctctgtg agtataattt
     1261 tccttatcac gagcaacatc aaaatgaatg acatagtcaa gattatcaat atcaatgccc
     1321 cgagcaacaa gatccgtagc taacaataag gagagttggt gggatttgaa tttttcgaga
     1381 atggttttac ggaatttgac attgatgtca ctcgctaagg aaaccgcagc agctccatta
     1441 tattggagac gttcttccgt agcacccaaa tcagataaac tgttgaaaaa gactaaagct
     1501 ctaaaatgag gaatattagc aaattttcgt aataaatcgg tacgttcgcg cttgtcaacc
     1561 atgaggtaaa aatgctgaat agcatcgttg gtttgctctg aaagatcgat gcagaatgta
     1621 tttggagcta aggaagtttg atccacctta ttagtagcac tcatgtatac catttgatga
     1681 tctcgaggaa catagtggct aattttttga acaaaatcat attgggaatc acctaatagt
     1741 tcgtcatatt catctaaaac aatggtgttg acagacatca tcttgatttt tttcaattta
     1801 atcagttcaa aaatgcgtcc aggggtccca atcaggattt caggaccttt tttgaggcgt
     1861 tcgatttgac gtttttgact ggttccagaa ataaagagtt gtgccgttaa gcctagtggc
     1921 tgtgcccaat cctttgtgac ttcaaaaatt tggccagcta attcggtatt aggggctaaa
     1981 atgagtagtt gttgggactt tttaggcgtc agtgccaata gactaggaaa gacataagct
     2041 aaggtcttgc ccgtcccagt agggctaata ccaaggaaat ttttaccatc aacaataggt
     2101 tgaaaggcct gttcttggat tggcgttaaa tgggtaaatg caacttggtc aagtttttct
     2161 tgccattgag ggggaaattt agtgatcatg cgtcttcctt ttactattgt ttctatattc
     2221 attatagcat tatagtctca attttggtaa aatagtgaga aagacttttt agatttggag
     2281 tttaaaatgc ttaaaaaacc gattattatt ggtgtaactg gtggttcagg tggcggtaaa
     2341 acaagtgtgt cacgcgctat tttggacagt tttccaaatg ctcgtattgc gatgattcaa
     2401 cacgattctt actataaaga tcagtctcac atgagttttg aagaaagggt caaaaccaat
     2461 tatgaccatc ctctagcttt tgatacagat tttatgattc agcaactaaa agaattatta
     2521 gctggtagac ctgtagatat tcctatctat gattacaaaa agcatacccg cagtaatacg
     2581 acctttcgtc aggatcctca ggatgttatt attgttgaag gtattcttgt cctagaagat
     2641 gagcgtttgc gagatttgat ggatatcaag ctctttgtag acacagatga cgatattcgt
     2701 attattcgtc gtattaagcg tgacatgatg gaacgtggca gaagcttaga aagtattatt
     2761 gatcagtaca caagcgtggt aaagcctatg tatcatcagt ttattgagcc aagtaaacgc
     2821 tatgctgata ttgttattcc agagggagtt agtaatgtcg ttgctattga tgttattaat
     2881 agtaaaattg cgagtattct tggagaagta tagtggaagt ggctgagtag attatttaaa
     2941 tctgctcttt tattgttgtg ctcttcatag agaaagggat cataaaatag aaatgttatt
     3001 aatttggctt ggattactat ttgtttggaa tgaactcgtg tttgtcatat atgcctttga
     3061 taaaagaaaa gccataaaaa agaaacggcg tattagtgaa agaaacttat tagtgataac
     3121 agttttgttt ggaggttttg gagcattact tgcagctaaa aagtaccacc ataaaaccag
     3181 aaagtggtac tttgtcatca cttggtatac tagtattttg ttgacattac tagtaactta
     3241 tatctttatg aagtgagtca cttaaaagac ccttaatcag tattatttag tcaaggctct
     3301 tttatagttt aaaaaagcac atcatcgtca agggtgttga ttttttgaaa aagatcacca
     3361 aagattaaaa ggattaatgt tcctagtaaa actgtcatgc caggaatgct tgttagagga
     3421 gagttccaga tgaaatgtag caccagagga cttaataacc agaaaatttg ttgctgtttt
     3481 gaaaaaaggt tagaaccctt taataataag tatgctccaa tggtaaaaat agctgtatgt
     3541 acccaatgtg aagtcacagc agtgctaata cgttcaatag cagtcggtac gagactatct
     3601 atagattttg cttgaatcaa ataacggata tcttctgcta gttgaaagcc catgccagaa
     3661 agtaaagcaa caactaattt ttctttaaga gtaagtctag gaaataaagt aattactaaa
     3721 atagccgaag aagccttaat gggctcttcg acaatagggg cagttaaagc atctccccaa
     3781 agctttaaag cgtctttaga agggatgatg tatgaccaaa attgtcctac gagatggttc
     3841 ccacaagaag ctaaaaaacc tgaaatataa agtccgctaa gacagctaac aataataaca
     3901 ttcaggttca tatgaaattt tttacttaaa taaaaaatag ctgctgcaaa tggaattaag
     3961 taaatgccta atagactaat ggttagtatt agagcaaaag cttctttaaa ggaaaggttg
     4021 ttagctgtca gtcctaacaa ttcaagttca ataccattta aaactaaagc aataaaaaga
     4081 ccgtatcgta accatttctg ttgagtgatt tgtttcacga aaacctccta atattaagat
     4141 gatattaata atattgaaaa ttatatcata aacaacctca aaaaccattt taaaaattga
     4201 ctgaagcgaa agatagtcgt aaaaaatact cgaatagacc cagatatgct ataataaaag
     4261 gtatgaataa gtctaaaaaa attgagcaat accaactaat gatagcacag gcaaaagagc
     4321 tgtttgcgaa tgaatcaaat gctttggcta atttgtcaaa tgccagcgcc ctgttaaaca
     4381 tgacactacc caattctgtt tttgcgggat tttacctttt tgatgggcag gaattaattc
     4441 ttggcccttt tcaaggtagg gtttcttgcg tccatatcaa gcttggtaaa ggtgtttgtg
     4501 gcgaatcagc tcagtctcgt cgaaccatta ttataaatga tgtgaagcag catgccaact
     4561 atatttcctg tgatgcggca gccatgagtg aaattgttgt gcctatggtg aaagaaggcc
     4621 atttgattgg ggtgctggac ttggattcta gccttgtagc tgattatgat gaggttgatc
     4681 aagaatactt agaggccttt gtggacctat ttcttgaaaa gaccacgttt acctttaaca
     4741 tgtttggagt taaaaactga tgtatcaagc tctttatcgg aaataccgga gccaaacgtt
     4801 tgacgaaatg gtgggacaat cggttatttc cacaacttta aagcaggcag ttgaatctgg
     4861 caagattagc catgcttatc ttttttcagg tcctagaggg actgggaaaa caagtgcggc
     4921 aaagattttt gcaaaggcca tgaattgtcc taaccaagtc gatggtgaac cctgtaatca
     4981 atgcgatatt tgccgagata tcacgaatgg aagcttggaa gatgtgattg aaattgatgc
     5041 tgcctcgaat aatggtgttg atgaaattcg tgacattcga gacaaatcaa cctatgcgcc
     5101 aagtcgtgcg acttataagg tttatattat tgatgaggtt cacatgttat caacaggggc
     5161 ttttaatgcg cttttgaaaa ctttggaaga accgacagaa aatgttgtct ttatcttggc
     5221 aacaacggaa ttgcataaaa ttccagccac tattttatct cgtgtgcaac gctttgaatt
     5281 caaagctatt aagcaaaaag ctattcgaga gcatttagcc tgggttttgg acaaagaagg
     5341 tattgcctat gaggtggatg ctttaaatct cattgcaagg cgagcagaag gaggcatgcg
     5401 tgatgcttta tctattttag atcaggcttt gagcttgtca ccagataatc aggtcaccat
     5461 tgcaattgcc gaagaaatta caggttctat ttccatgctt gctctgggtg actatgttcg
     5521 atatgtctcc caagaacagg ctacgcaagc tctggcagcc ttagagacca tttatgatag
     5581 tgggaagagc atgagccgct ttgcgacaga tttattgacc tatctgcgtg atttattggt
     5641 ggttaaagct ggcggtgaca atcagcgtca gtcagctgtt tttgatacca atttgtctct
     5701 ctcgatagat cgtatattcc aaatgataac agttgttact agtcatctcc ctgaaatcaa
     5761 aaagggaacc catcctcgga tttatgccga aatgatgact attcagctag ctaaagaaga
     5821 aggagtagcg ttaccatcag gtttgtcatc agatgtgacg actgaagttg agggacttaa
     5881 acgtgaactt aatcagctta agcaagcctt ggcacaattg caagccagtc ccagtccctc
     5941 acatgctcct gtaaaaccag cacaggctaa accgaaacta accacttaca agattgatcg
     6001 ggtgaaaata ctgaaaatca tggaagaaac ggttcaagat agtcagcaat cacgccaata
     6061 cttggatgcc ctaaaaacag cttggaatga aattttagac agtatctcag ctcaagatag
     6121 agccttacta atgggatcag agcctgttct ggccaatagt gagaatgcta ttttagcttt
     6181 tgaagctgct tttaatgctg agcaagcaat gaagcgtact gatttgaatg cgatattcgg
     6241 caacattatg agcaaagcag caggcttctc acctaatatt ttggcagtcc ctaaagctga
     6301 ttttcatcac attcgttctg agtttgcctt acaattgaaa actcaaaagg cttctgaccc
     6361 tacagaagag acgatagaag aaacccttga gattccagaa ggctttgatt ttctagttga
     6421 taaaattaat gccgttgagg attaaacatt attagctgtt aatgaaccag taacgctata
     6481 aaggtaagcc tattgggctt acctttagcg tttttttatc taatatacca agatagtttt
     6541 tctagccaat cctctaatag tagaagtgaa accaaaaaag attgatgctt tcttgggtat
     6601 ccaagtcagc atcaatctgc acgccaaacg catgaagaaa cttttgtttt cagaaaacta
     6661 gattcataat gacaaaatca atcaaagtag tacactatag atgaggtgac tacgatgatt
     6721 gattttatta tttctattga tgattgcgca gttgaattgg atagtcgtca atcttggaaa
     6781 att
//
LOCUS       DATXIG010000071         6577 bp    DNA     linear   BCT 28-DEC-2023
DEFINITION  TPA_asm: Streptococcus pyogenes strain MGAS11113
            SAMN08555799-rid19682583.denovo.071, whole genome shotgun sequence.
ACCESSION   DATXIG010000071 DATXIG010000000
VERSION     DATXIG010000071.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN08555799
            Sequence Read Archive: SRR6740495
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pyogenes
  ORGANISM  Streptococcus pyogenes
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 6577)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6577)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-OCT-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-OCT-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001918058.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001918058.2
            Genome Coverage       :: 368x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/12/2023 11:23:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 1,806
            CDSs (total)                      :: 1,769
            Genes (coding)                    :: 1,722
            CDSs (with protein)               :: 1,722
            Genes (RNA)                       :: 37
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1, 1 (5S, 23S)
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 47
            CDSs (without protein)            :: 47
            Pseudo Genes (ambiguous residues) :: 0 of 47
            Pseudo Genes (frameshifted)       :: 30 of 47
            Pseudo Genes (incomplete)         :: 20 of 47
            Pseudo Genes (internal stop)      :: 9 of 47
            Pseudo Genes (multiple problems)  :: 11 of 47
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6577
                     /organism="Streptococcus pyogenes"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN08555799-rid19682583.denovo.071"
                     /strain="MGAS11113"
                     /serotype="Emm28"
                     /isolation_source="normally sterile site"
                     /host="Homo sapiens"
                     /db_xref="taxon:1314"
                     /country="Finland"
                     /collection_date="2002"
                     /collected_by="National Public Health Laboratory"
     gene            complement(<1..110)
                     /locus_tag="VC122_001524"
     CDS             complement(<1..110)
                     /locus_tag="VC122_001524"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010921949.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEP5641441.1"
                     /translation="MLRTNTIAGNFFIDGQQQKTLINPIKIDKDIVTIQE"
     gene            complement(151..1176)
                     /locus_tag="VC122_001525"
     CDS             complement(151..1176)
                     /locus_tag="VC122_001525"
                     /EC_number="3.4.22.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010922160.1"
                     /note="This family includes IgM or IgG-cleaving cysteine
                     proteases; Derived by automated computational analysis
                     using gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IdeS/Mac family cysteine endopeptidase"
                     /protein_id="HEP5641442.1"
                     /translation="MRKRCYSTSAAVLAAVTLFALSVDRGVIADSFSANQEIIYSEVT
                     PYHVTSVWTKGVIPPANFTQGEDVFHAPYVANQGWYDITKTFNGKDDLLCGAATAGNM
                     LHWWFDQNKEKIEAYLKKHPDKQKIMFGDQELLDVRKVINTKGDQTNSELFNYFRDKA
                     FPGLSARRIGVMPDLALDMFINGYYLNVYKTQTTDVNRTYQEKDRRGGIFDAVFTRGD
                     QSKLLTSRHDFKEKNLKEISDLIKKELTEGKALGLSHTCANVRINHVINLWGADFDSN
                     GNLKAIYVTDSDSNASIGMKKYFVGVNSAGKVAISAKEIKEDNIGAQVLGLFTLSTGQ
                     DSWNQTN"
     gene            1680..1882
                     /locus_tag="VC122_001526"
                     /pseudo
     CDS             1680..1882
                     /locus_tag="VC122_001526"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003051153.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside-diphosphate kinase"
     gene            2084..2464
                     /locus_tag="VC122_001527"
     CDS             2084..2464
                     /locus_tag="VC122_001527"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002985011.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1149 family protein"
                     /protein_id="HEP5641443.1"
                     /translation="MQLVREKEFVNQYHYDARNLEWEKENGTPETNFEVTFQLIDKDE
                     QQKETVIVSVLQFVIVKEEFVISGVISQMVRILDRLVDKPSEFTQEEVESLAAPLLDM
                     VKRLTYEVTEIALDRPGIHLEFKN"
     gene            2464..3312
                     /locus_tag="VC122_001528"
     CDS             2464..3312
                     /locus_tag="VC122_001528"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002985007.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DegV family protein"
                     /protein_id="HEP5641444.1"
                     /translation="MKLAVITDSTATLPTDLKQDKAIFSLDIPVIIDDETYFEGRNLS
                     IDDFYQKMADSQNLPKTSQPSLSELDNLLGLLSSKGYTHVIGLFLAGGISGFWQNIQF
                     LAEEHPEIEMAFPDSKITSAPLGSMVKNVLDWSRQGMTFQAILNKLQEQIDGTTAFIM
                     VDDLNHLVKGGRLSNGSALLGNLLSIKPILRFDEEGKIVVYEKVRTEKKAMKRLVEIL
                     NDLIADGQYNVSIIHSKAQDKADYLKRLLQDSGYQYDIEEVHFGAVIATHLGEGAIAF
                     GVTPRL"
     gene            3437..4645
                     /locus_tag="VC122_001529"
     CDS             3437..4645
                     /locus_tag="VC122_001529"
                     /EC_number="2.7.7.72"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009880307.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CCA tRNA nucleotidyltransferase"
                     /protein_id="HEP5641445.1"
                     /translation="MKLMTMPSEFQKALPILTKIKEAGYEAYFVGGSVRDVLLERPIH
                     DVDIATSSYPEETKAIFNRTVDVGIEHGTVLVLENGGEYEITTFRTEDVYVDYRRPSQ
                     VSFVRSLEEDLKRRDFTVNALALDENGQVIDKFRGLIDLEQKRLRAVGKAEERFEEDA
                     LRIMRGFRFAASLDFDIEAATFEAMRSHSPLLEKISVERSFTEFDKLLMAPHWRKGIS
                     AMIACQAYDYLPGLKQQEAGLNHLIVSLKDNFTFSDHHQAWAYVMISLAIEDPKSFLK
                     AWKTSNDFQRYVTKLIALYRIRQERSFEKLDIYQYGKEMASLVEGLRKAQSLSVDMDH
                     IEALDQALAIHNKYDIVLNGSHLIKDFGMKPGPQLGLMLEKVELAIVEGRLDNDFTTI
                     EAFVREELAT"
     gene            4642..6519
                     /locus_tag="VC122_001530"
     CDS             4642..6519
                     /locus_tag="VC122_001530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010922163.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC-F family ATP-binding cassette
                     domain-containing protein"
                     /protein_id="HEP5641446.1"
                     /translation="MSHFLVKKLTKTVGDKTVFQDISFIIHDFDRIGIIGVNGTGKTT
                     LLDVLSGRLGFDGDHSPFSKANDYKIAYLTQDPEFNDASSVLDTVLSSDVKAIQLIRQ
                     YELLMANYTEDKQETLESLMSEMDRLDAWSIESDVKTVLSKLGITDLEQKVGDLSGGM
                     RRRVQLAQVLLGAADLLLLDEPTNHLDIDTIAWLTTYLKTAKKTVLFITHDRYFLDHV
                     ATRIFELDKAGLTEYQGNYQDYVRLKAEQDERDATNLHKKKQLYKQELAWMRTQPQAR
                     ATKQQARINRFTDLKKEVHQDSSADKLEMAFETSRIGKKVIHFEDLSFAYGDRQLIKD
                     FNLIIQNKDRIGIVGDNGVGKSTLLNIINGDLKPTSGKLDIGDTIRVGYFSQQLKDMD
                     ETKRVINYLQEVADEVKTSVGTTSISELLEQFLFPRSSHGTLIAKLSGGEKKRLYLLK
                     LLIEKPNVLLLDEPTNDLDIATLTVLENFLANFAGPVITVSHDRYFLDKVATKILAFE
                     EGDIRIFYGSYSDYLDEKAFEKETVEADLAKAIVTEEVPLPPKEEKKRMSYLEKQEWA
                     QIEDKIATIEANIEEIENQMLTVVSDYGQLAQLQKELDQRNNDLLLAYERFEYLSGLD
                     I"
BASE COUNT         2114 a         1085 c         1366 g         2012 t
ORIGIN      
        1 aactcttgaa tcgtaacaat atccttatcg atttttatag gatttattaa tgttttttgc
       61 tgttgcccat ctataaaaaa attccctgca atagtattag tccttagaat accatcatct
      121 gttgaatgta atctttctta ttgatacgct ttaattggtc tgattccaac tatcttgccc
      181 tgttgaaagt gtaaataacc ctagtacttg agcaccaata ttatcttctt ttatttcttt
      241 agcagaaata gctacttttc cagcggagtt aacaccaaca aagtatttct tcataccaat
      301 agatgcatta ctatcagagt ctgttacata aatagcttta aggttcccgt tagaatcaaa
      361 gtcagctccc cacaggttta taacatggtt gatgcgtacg ttagcgcagg tgtgtgatag
      421 gcctagagcc ttgccttcgg ttaactcttt cttaatgaga tcactgattt ctttgagatt
      481 tttttcttta aaatcatgac gacttgtcaa tagcttactt tgatcacctc ttgtaaatac
      541 ggcgtcaaaa ataccacctc ggcgatcttt ctcttgatag gttctattga catcagtagt
      601 ctgtgtctta taaacattta agtaataacc attgataaac atatctaaag caagatcagg
      661 cataactcca attcggcgtg ctgacaaacc ggggaaagct ttatctcgga aataattaaa
      721 aagctcgcta tttgtttggt cacctttagt attaataact tttcttacat ccaataattc
      781 ttgatcacca aacatgattt tttgtttatc tgggtgtttt tttagatatg cttcaatttt
      841 ttctttgttt tgatcgaacc accagtgaag catattccct gctgtggcag ccccgcaaag
      901 aagatcgtct tttccattga atgttttggt aatatcatac catccttggt tagcaacata
      961 aggagcgtga aaaacatctt cgccttgagt gaagtttgct ggaggaataa ctcctttggt
     1021 ccaaacggaa gtaacgtgat aaggtgttac ttccgaatat ataatctctt gattagcaga
     1081 aaaactatct gctataacac cacgatctac cgatagagca aataaagtca ctgctgccaa
     1141 tactgcagct gaagttgaat agcatctttt tctcataaga ataccttttt ctatataatt
     1201 ttattataaa aagattataa catatagaca tctattgttg caatttttta aaataatact
     1261 taatttttta aataattttt aataaacgta aacaagcgat tgacaatgga gatatcacaa
     1321 gagatacagt agtggttgtg cttaaaatgg acggtttttt gatgatatgt tgccgcatga
     1381 ggaagtgagc aataggagag ctgtgataga agaaagagag gaagaagcgt tgatgaaatt
     1441 agacaaataa aaaaaaagac ctcatccaga agaggtcgtg gtataactgg ggacgatagg
     1501 ttcaattgat aaaaaatctt gttagcttac gatctatgtt gttttgaatg cctttaactc
     1561 aacatcgtta atggattttg agataaaaaa ataaatccaa tagtctggta ctttcctatt
     1621 ttttgtctgt taaaacgttt ttattttata atggataaaa ggagaagaag ggcggagcga
     1681 tgattaagct ctatgggttt aagtatgggt tagttggaga ggttttgcgg cggattgaac
     1741 gcctaggatt tacgtttgag cgattagagc taagacaagc tagttcgaaa tacttggcta
     1801 agcatgatga ggccttggtg ataaaccttt ttatcctgga cttaaaactt acctgaccag
     1861 tggttctgtt ttaattgagg tgcagtacaa gaccactaac gggtgttttt ttgatataat
     1921 gtcagtataa ggacgactaa atataaatag gtgaaaaaat agatatctgt tcgagagata
     1981 gcactgttta aaacgttatg cgaaaatagt agtttagggg ctactttaat aatcttcaaa
     2041 tctatgctat aatggatcat attgatacca aggagaataa aaaatgcaat tagttcgtga
     2101 aaaagaattt gtcaaccagt accattatga tgctagaaat ttagagtggg aaaaagaaaa
     2161 cggaacacca gagaccaatt ttgaagtgac ttttcagttg attgataaag atgagcagca
     2221 aaaagaaaca gtaattgtat ctgtattgca atttgttatt gtcaaagaag aatttgttat
     2281 tagtggggtc atctcacaaa tggttcgtat tttagatcgt ttagtggata aaccaagcga
     2341 atttacgcaa gaggaagttg aatccttagc tgctcctttg ctagacatgg ttaagcgatt
     2401 gacttatgaa gtcacagaaa ttgccttgga tcgtccaggt attcatttgg agtttaaaaa
     2461 ttaatgaaat tagcagtaat tactgacagt acagcaacac taccaacaga tctgaaacaa
     2521 gataaagcta tttttagcct tgatatacca gttatcattg acgatgagac ctattttgaa
     2581 ggtcgaaatt tgagtataga tgatttttat caaaagatgg ctgactccca aaatcttccc
     2641 aagacaagtc agccaagttt atctgaacta gataatcttt taggactttt gtcttctaag
     2701 ggctatacac atgttattgg cttgttctta gctggaggaa tctcaggctt ttggcaaaat
     2761 attcaatttt tagctgaaga acatcctgag attgaaatgg catttccaga cagtaagatc
     2821 acatctgctc ctttaggtag tatggtaaaa aatgtgctag attggtcacg tcaaggaatg
     2881 acatttcaag ctattttaaa caagctgcaa gaacagattg atgggacgac agcctttatt
     2941 atggttgatg accttaatca tctggttaaa ggcggacgcc tgtcaaatgg gtctgctctt
     3001 cttggaaatc ttttgagtat taaacctatc ttacgctttg atgaagaggg aaaaatcgtt
     3061 gtttatgaaa aagtgagaac agaaaagaaa gccatgaaac gcttggttga gattttaaat
     3121 gacttgatag cagacggtca gtataatgtg tctattattc actcaaaagc acaagacaaa
     3181 gctgattatt tgaaacgact cttacaagac agtggttatc aatatgacat tgaagaggtt
     3241 cattttggag cggtgattgc tactcattta ggagaaggag ccattgcatt tggcgtaaca
     3301 ccgaggcttt aattgtcgat tagaaaaaat agttaattgc aagaatcact aaaacaataa
     3361 caaaatgtga tagaacccaa gtagcctaat cacactttag attgggctac ttggatgata
     3421 ggttagaaaa caacgtatga aattaatgac aatgccttct gagtttcaga aggctttacc
     3481 catattgact aaaataaaag aagctggtta tgaagcctac tttgtgggag gaagcgtcag
     3541 agatgtttta ctcgagcgtc ccattcatga tgtagatatt gcaactagtt cctatcctga
     3601 agagactaaa gcgattttta acagaactgt cgatgttggc attgaacatg gtacagtgct
     3661 cgtgcttgaa aatggcggtg agtatgaaat cacaaccttt aggacagaag atgtctatgt
     3721 tgactatcgc cgtcctagtc aggtttcttt tgtgcgctct ttagaggaag acttaaaacg
     3781 aagagacttt accgtcaatg ccttggcctt ggatgaaaat ggtcaagtta ttgataaatt
     3841 tagaggactc attgatttgg agcaaaaacg gttacgagca gtgggtaaag cagaggaacg
     3901 ttttgaagaa gatgcactac gcattatgag aggctttcga tttgcggcga gtctggattt
     3961 tgacattgaa gctgcaactt ttgaagctat gaggagccat tcacctctat tggaaaagat
     4021 ttctgtggaa cgttctttta ctgaatttga taaattattg atggcaccgc actggcgaaa
     4081 agggatttct gccatgattg cttgtcaggc ttatgactat ttaccaggct tgaagcagca
     4141 ggaagcaggc ttgaatcatc tgatagtttc tttgaaagat aactttacct ttagtgatca
     4201 ccaccaagct tgggcttatg tgatgattag tttagccatt gaggatccta aaagcttcct
     4261 aaaagcatgg aagacttcaa atgattttca gcgttatgtc acaaagttga ttgctcttta
     4321 tagaattcgt caggaaagat cttttgaaaa attggatatt taccagtacg gcaaggaaat
     4381 ggcaagtcta gtggaaggcc tccgaaaagc tcaaagctta tctgttgaca tggaccacat
     4441 tgaagcttta gatcaagcgc tagccattca taacaaatac gatatcgtcc ttaatggttc
     4501 tcacctgatt aaggattttg ggatgaaacc aggtcctcag ctagggctta tgcttgagaa
     4561 agttgaatta gcgattgtag agggacgcct agataatgat ttcactacaa ttgaagcatt
     4621 tgttagagag gaattagcaa catgagccat tttttggtga agaaattgac aaaaacggtg
     4681 ggagataaaa cagtctttca ggatatttcc tttattattc atgactttga ccgtattggg
     4741 attattgggg ttaatggaac aggaaaaaca actcttttag atgtcttgtc gggaaggctt
     4801 ggttttgacg gagaccattc accattttct aaagccaatg attacaaaat cgcttactta
     4861 acacaagatc ctgaatttaa cgatgcatcc tctgttttag atacggtatt gtcatctgat
     4921 gttaaggcga ttcagctgat tcgtcagtat gaactattga tggcgaatta tactgaagat
     4981 aaacaagaga ccttagagtc cctcatgtct gaaatggatc gcttagatgc ttggtctatt
     5041 gaaagtgatg tcaaaacagt cctatcaaaa ttaggcatca cggatttgga acaaaaagtg
     5101 ggagacctat caggtggtat gagaagacgt gtccagttag ctcaagtatt gctaggagct
     5161 gctgacttac tcttgttgga tgagccgacc aaccatctcg atattgacac catcgcttgg
     5221 ttaacaacct atctcaagac agctaaaaaa accgtccttt ttattaccca tgaccgttat
     5281 ttccttgatc atgtggctac ccgtattttt gaattggata aagcgggact gacagaatat
     5341 caaggcaatt accaagacta tgtgcgctta aaagcagaac aagacgaacg agatgctact
     5401 aatttacaca agaagaagca actctataaa caagaattag cttggatgag gacgcagcca
     5461 caggcgcgtg caaccaaaca gcaggctaga atcaatcgtt tcactgactt gaaaaaagaa
     5521 gttcaccaag acagttctgc agataaattg gagatggctt tcgaaacgag ccgaattggg
     5581 aagaaggtga ttcactttga agacctttct tttgcttatg gcgaccgaca gttgattaag
     5641 gattttaact tgattattca aaataaagat cgtattggaa ttgttggaga taacggcgtc
     5701 ggaaaatcta ccctgttaaa tatcatcaac ggtgatctca aaccaacttc tgggaagctt
     5761 gatattggag atactattcg tgtaggttat ttctctcagc agctcaaaga tatggatgag
     5821 accaaacggg tcattaatta ccttcaagaa gtggctgatg aggttaaaac aagcgtggga
     5881 acaacgagta tcagtgagct tttagagcaa tttctttttc cgagatcaag tcatggcacg
     5941 ctgattgcta aattatctgg tggggagaaa aaacggctct acttgctaaa actgctcatt
     6001 gaaaagccaa atgttcttct ccttgatgag ccaacaaatg atttagacat tgccaccttg
     6061 acggtcttag aaaacttttt agctaacttt gcaggaccag tcattacggt tagtcacgat
     6121 cgttatttcc tagacaaagt agccactaag attttagcct ttgaagaagg cgatattcgt
     6181 attttttatg ggagttacag tgattacctt gatgaaaaag cttttgaaaa agaaacagtt
     6241 gaggctgact tagctaaagc aatagtcact gaagaagtgc cacttcctcc gaaagaggag
     6301 aaaaagcgca tgtcctacct tgaaaaacaa gaatgggctc agatagaaga taaaatagca
     6361 actattgagg ctaatattga agaaattgaa aaccaaatgt taactgtagt tagtgattat
     6421 ggccagttag ctcaattaca aaaggaactc gatcagcgca ataatgattt attattggcg
     6481 tatgaacggt ttgagtattt gagtggacta gacatataag tatactatgg taatgttatg
     6541 tttataataa acaagtgata atagaaaaga agtgctc
//
LOCUS       DATXIG010000072         6337 bp    DNA     linear   BCT 28-DEC-2023
DEFINITION  TPA_asm: Streptococcus pyogenes strain MGAS11113
            SAMN08555799-rid19682583.denovo.072, whole genome shotgun sequence.
ACCESSION   DATXIG010000072 DATXIG010000000
VERSION     DATXIG010000072.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN08555799
            Sequence Read Archive: SRR6740495
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pyogenes
  ORGANISM  Streptococcus pyogenes
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 6337)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6337)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-OCT-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-OCT-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001918058.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001918058.2
            Genome Coverage       :: 368x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/12/2023 11:23:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 1,806
            CDSs (total)                      :: 1,769
            Genes (coding)                    :: 1,722
            CDSs (with protein)               :: 1,722
            Genes (RNA)                       :: 37
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1, 1 (5S, 23S)
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 47
            CDSs (without protein)            :: 47
            Pseudo Genes (ambiguous residues) :: 0 of 47
            Pseudo Genes (frameshifted)       :: 30 of 47
            Pseudo Genes (incomplete)         :: 20 of 47
            Pseudo Genes (internal stop)      :: 9 of 47
            Pseudo Genes (multiple problems)  :: 11 of 47
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6337
                     /organism="Streptococcus pyogenes"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN08555799-rid19682583.denovo.072"
                     /strain="MGAS11113"
                     /serotype="Emm28"
                     /isolation_source="normally sterile site"
                     /host="Homo sapiens"
                     /db_xref="taxon:1314"
                     /country="Finland"
                     /collection_date="2002"
                     /collected_by="National Public Health Laboratory"
     gene            142..1413
                     /locus_tag="VC122_001531"
     CDS             142..1413
                     /locus_tag="VC122_001531"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002990240.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter periplasmic adaptor
                     subunit"
                     /protein_id="HEP5641447.1"
                     /translation="MSKRGKIKITTKTKLITASVITLVLIITGVVLWKQQQNTLTADI
                     AKEPYSTVSVTEGSIASSTLLSGTVKALSEEYIYFDANKGNDATVTVKVGDQVTQGQQ
                     LVQYNTTTAQSAYDTAVRSLNKIGRQINHLKTYGVPAVSTETNKDEATGEETTTTVQP
                     SAQQNANYKQQLQDLNDAYADAQAEVNKAQIALNDTVVISSVSGTVVEVNNDIDPSSK
                     NSQTLVHVATEGQLQVKGTLTEYDLANVKVGQSVKIKSKVYSNQEWTGKISYVSNYPT
                     ESNAGSTTPAGSTGAGSSTGAAYDYKIDIISPLNQLKQGFTVSVEVVNEAKQALVPLT
                     AVIKKDKKHYVWTYDDATGKAKKVEVTLGNADAQQQEIHKGVAVGDIVIANPDKNIKP
                     DKKLEGVISIGTNTKPEKDSQSKNKKSGVDK"
     gene            1413..2123
                     /locus_tag="VC122_001532"
     CDS             1413..2123
                     /locus_tag="VC122_001532"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002985080.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEP5641448.1"
                     /translation="MSHEKKQLMQLSNIVKSYQNGDQVLKVLKGINLTVYEGEFLAIM
                     GPSGSGKSTLMNIIGLLDRPTSGDYTLHNTKIEILNDRELAKVRNDEIGFVFQQFFLL
                     AKLTALQNVELPLIYAGVNVSKRREQAKQFLEKVGLGRRIKHLPSELSGGQKQRVAIA
                     RALVNDPSIILADEPTGALDTKTGQQIMELLTELNKEGKTIIMVTHEPEIADFATRKI
                     IIRDGDITTDTTASVVID"
     gene            2135..3355
                     /locus_tag="VC122_001533"
     CDS             2135..3355
                     /locus_tag="VC122_001533"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011017655.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEP5641449.1"
                     /translation="MENWKFALSSIWGHKMRSILTMLGIIIGVAAVVIIMGLGNAMKN
                     SVTSTFSSKQKDIQLYFQEKGEEEDLYAGLHTHENNHEVKPEWLEQIVKDIDGIDSYY
                     FTNSATSTISYEKKKVDNASIIGVSKDYFNIKNYDIVAGRTLTDNDYSNFSRIILLDT
                     VLADDLFGEGNYKSALNKVVSLSDKDYLVIGVYKTDQTPVSFDGLSGGAVMANTQVAS
                     EFGTKEIGSIYIHVNDIQNSMNLGNQAADMLTNISHIKDGQYAVPDNSKIVEEINSQF
                     SIMTTVIGSIAAISLLVGGIGVMNIMLVSVTERTREIGLRKALGATRLKILSQFLIES
                     VVLTVLGGLIGLLLAQLSVGALGNAMTLKGACISLDVALIAVLFSASIGVFFGMLPAN
                     KASKLDPIEALRYE"
     gene            3609..5342
                     /locus_tag="VC122_001534"
     CDS             3609..5342
                     /locus_tag="VC122_001534"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002990233.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerophosphoryl diester phosphodiesterase
                     membrane domain-containing protein"
                     /protein_id="HEP5641450.1"
                     /translation="MTFLSDLISLMTKIRLSWVIKAGIFQLLFVTIANIVLSEFFYFI
                     LDVTGQYHLDKDNVVTFLKNPIALALLGAYLFLLAAFIHLEFFALYRIIADQEISFYL
                     FRKQFSYYLRGLWKTFSGYQLLLFLLYILLTIPVLHIGLSSVITQKLYLPEFIVGELS
                     KITSTKYLLYGSLILVFYLNLRLVYFLPLIAINHRTVAQAWRESWQKTKKKHVLLWMK
                     LFAINGLTLVVVSLAISMILIFVDMFNPKGNNIIVQLGALTFTWELIFFTTIFFKLCS
                     AMILKEAIEPQKQYDEPRRSNKAYVVIFIVVTVGFAYQSLERLTFFDTSHSKTVIAHR
                     GLVSAGVENSLEALEGAKKAGSDYVELDLILTKDNHFVVSHDNRLKRLAGVNKTIRNL
                     TLKEVEHLTSHQGHFSGRFVSFDTFYQKAKKLNMPLLIELKPIGTEPGNYVDLFLETY
                     HRLGISKDNKVMSLDLEVIEAIKKKNPSITTGYIIPIQFGFFGDEFVDFYVIEDFSYR
                     SYLSSQAFWNNKEIYVWTINDPKRIEHYLLKPIQGIITDQPALTNQLIKDLKQDNSYF
                     SRLVRIISSLY"
     gene            5469..5741
                     /gene="rpsP"
                     /locus_tag="VC122_001535"
     CDS             5469..5741
                     /gene="rpsP"
                     /locus_tag="VC122_001535"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002884675.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S16"
                     /protein_id="HEP5641451.1"
                     /translation="MAVKIRLTRMGSKKKPFYRINVADSRAPRDGRFIETVGTYNPLV
                     AENQITIKEDRVLEWLSKGAQPSDTVRNILSKAGVMAKFHDQKFSK"
     gene            5751..5990
                     /locus_tag="VC122_001536"
     CDS             5751..5990
                     /locus_tag="VC122_001536"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005590445.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="KH domain-containing protein"
                     /protein_id="HEP5641452.1"
                     /translation="MDTIENLIIAIVKPLISQPDNLTIKIEDTPDFLEYHLDLDAQDI
                     GRVIGKKGRTITAIRSIVYSVPTLGKKVRLVIDEK"
BASE COUNT         2127 a          959 c         1201 g         2050 t
ORIGIN      
        1 cagtaagcta ttaatttgtc atagcatata agtcattatc ttgtggcttg ttttcgacta
       61 agggttaaga atttcttgac aacaatataa aaaggtcata tacttgtctt atacaaatag
      121 tacaaaaagg ggaggataga aatgtctaaa agaggaaaaa ttaaaataac aacgaaaaca
      181 aagcttatta cagctagtgt tataacgcta gtattaatta taactggagt agtcttgtgg
      241 aaacaacaac aaaatacgct aacagctgat atcgctaaag aaccttactc tactgttagt
      301 gtaactgaag ggagtattgc ttcttcgact ttactctcag gtactgtaaa ggctttatca
      361 gaggaatata tttatttcga tgctaataaa ggaaatgatg caactgttac agttaaagta
      421 ggtgatcagg taacgcaggg ccagcaatta gttcaatata atacaacaac agctcagtca
      481 gcttatgata ctgctgttag gagtcttaac aagattggcc gacaaattaa tcatcttaaa
      541 acatacggag ttcctgctgt tagtacagaa actaataaag atgaagctac cggtgaagag
      601 acgacaacaa cagttcaacc atcagctcag caaaatgcta attataaaca gcagctgcaa
      661 gatttaaatg atgcttatgc agatgcacaa gcagaagtaa ataaagcgca gatagcgtta
      721 aatgatacag tagttatcag tagtgtctct ggaactgttg tggaagtaaa taatgatatt
      781 gatccttctt caaagaacag tcaaacactt gttcacgtag caaccgaagg acagcttcag
      841 gtgaaaggaa cattgacaga gtatgattta gcaaacgtta aggttggtca atctgtaaaa
      901 attaagtcta aagtttattc taatcaagaa tggactggaa aaatatcata tgtttcaaac
      961 tatccaactg agtctaatgc aggttcaaca acgccagcag gtagcactgg agcgggaagt
     1021 tctacaggag ctgcctatga ttacaagatt gatattataa gtcctctcaa ccagcttaaa
     1081 caaggtttca ctgtttctgt tgaggttgtt aatgaagcta aacaggcctt agttccttta
     1141 acggctgtta ttaagaaaga taaaaaacac tatgtttgga cttatgatga tgctactggc
     1201 aaagccaaaa aagtagaggt gacacttgga aacgcagatg cacaacaaca agaaattcat
     1261 aaaggagtag ctgttggtga cattgttatt gccaatccag ataaaaatat caaaccggat
     1321 aaaaaactag aaggggttat ttcaataggt accaacacaa aaccggaaaa agattctcaa
     1381 tcaaagaata aaaaatcagg ggtggataaa tagtgtcgca tgaaaaaaaa caattaatgc
     1441 aattatcaaa tattgtgaag agctatcaaa atggggatca agttcttaaa gttttgaaag
     1501 gtattaattt aacagtatat gagggtgaat ttttagctat tatgggtccg tctggctcgg
     1561 gaaaatcaac actaatgaat attattggtt tattagatcg gccaacatcc ggagattaca
     1621 cattacataa tactaaaata gaaatcctta atgatcgtga attagcaaaa gtacgaaacg
     1681 atgaaatcgg ctttgtcttt caacaattct ttctgttagc taaattaact gcattacaaa
     1741 atgttgagtt gcctcttatt tatgctggtg taaacgtctc aaaaagacgt gaacaggcta
     1801 agcaatttct tgaaaaagta ggacttggac gacgtatcaa acatctaccg tcagaactgt
     1861 caggaggtca aaagcaaagg gtggctatcg cacgagcttt agtaaatgac ccatctatta
     1921 ttttagcgga tgaaccaaca ggtgcactag atactaaaac aggtcagcaa attatggaac
     1981 tcttgacaga gttaaacaaa gaaggtaaaa caattattat ggttactcat gaaccagaaa
     2041 ttgctgattt tgcaactcgg aaaattatta ttcgtgatgg tgatatcaca acggatacta
     2101 cagctagtgt tgttattgac taggaggaaa aataatggaa aattggaaat tcgctcttag
     2161 ctcaatttgg gggcataaga tgcgctcaat cctgacaatg ttgggaatta tcatcggtgt
     2221 agcagcagtt gtgattatta tggggttggg aaatgctatg aaaaatagtg taacaagcac
     2281 attttctagc aagcaaaaag atattcaact ctattttcaa gaaaaaggag aagaagaaga
     2341 cctgtatgcc ggattgcaca cgcatgaaaa taatcacgaa gtgaagccag agtggttaga
     2401 acaaattgtt aaagatatcg atggtattga ttcttattac tttaccaatt ctgctacttc
     2461 tactatttcg tatgaaaaaa agaaagtaga caatgcttcg atcatagggg taagtaaaga
     2521 ttattttaac ataaaaaact atgatatcgt agctggtcgt acactaacag ataatgatta
     2581 ttctaatttt tcgcgtatca ttcttttaga tacagtatta gccgatgacc tatttggcga
     2641 aggtaattat aagtcagctt taaataaagt ggtaagttta tctgataaag attatttagt
     2701 tattggtgtt tataaaactg atcagacgcc agtttctttt gatggattat caggcggcgc
     2761 tgtcatggca aatactcagg tagcttcaga gtttggcacc aaagaaatag gatctatcta
     2821 cattcatgtg aacgatattc aaaacagtat gaacttagga aatcaagcag ctgatatgct
     2881 aaccaacata tcgcatatta aagatggaca gtacgctgtt cctgataata gcaaaatcgt
     2941 tgaagaaatc aatagtcagt tcagtatcat gacaacagtc ataggttcaa ttgcagcgat
     3001 ctctctttta gtgggaggaa ttggtgttat gaatattatg ttggtatctg tcacagagcg
     3061 aactagagaa attggtttac gtaaagcact tggtgcaaca cgcctgaaaa ttttatcaca
     3121 atttttgatt gaatccgttg ttctaacagt tcttggaggc ttgataggat tattattagc
     3181 tcaattaagt gtaggggctt taggaaatgc gatgacatta aaaggagctt gtatttctct
     3241 tgatgtggcc ttaatagcag ttttattttc tgcctcaata ggtgtctttt ttggaatgtt
     3301 gccagctaat aaagctagta agttagatcc aattgaggca ttacgttatg aatagaatag
     3361 ttcgtatcaa ttaatctata gacgctgact agtttaacgt ctgattatat ctagtttttg
     3421 tattagcaca gtagttatat cattgaagtg ttagtgcatt ttttgaggtg ataatatcta
     3481 gcaaagctat cattggtatg atgatgtgga tagcgtataa gtgatacatc tcttatttct
     3541 agctgaactt cggttcggct aggaagttgt caggcaatga gttgtttaaa ataaaggaga
     3601 ttagttcaat gacattttta tctgatttga tatcattaat gacaaaaatc agattatctt
     3661 gggtaataaa ggcgggtatt tttcaattat tatttgtaac gattgctaat attgtcttat
     3721 cagaattttt ctattttata ttagacgtta ctggtcaata tcatttagat aaagacaatg
     3781 ttgtgacttt tttaaaaaat cctatagcac ttgctttatt aggtgcctat ttatttttat
     3841 tagctgcttt tattcacctt gagttttttg ctctatatcg aattattgcg gatcaagaaa
     3901 ttagtttcta tctttttaga aaacagtttt cttattacct aagggggctt tggaaaacat
     3961 tttctggtta ccaattatta ctttttttgc tttatatcct attgactatt ccagtcttac
     4021 atattggttt atcttctgtg attactcaaa agctttatct tccagaattt attgttgggg
     4081 aattatcaaa gataactagc acaaagtact tgctttatgg cagtcttatt cttgtatttt
     4141 accttaacct aagattagta tattttttac cattgatagc aatcaaccat cgtacggttg
     4201 ctcaagcatg gagagagagt tggcaaaaga ctaaaaagaa acatgtattg ttatggatga
     4261 aactttttgc aatcaatggt cttacgcttg tagtcgtatc gctagctatt tccatgattc
     4321 ttatttttgt tgatatgttt aatcctaagg ggaataatat tattgttcag ctgggagctt
     4381 tgacctttac atgggagctc atttttttta ctactatttt ttttaaactc tgttcagcaa
     4441 tgattttaaa agaggcaatt gaaccacaaa agcaatatga tgagccaaga agaagtaata
     4501 aggcatatgt tgtaatcttt atcgtggtga cagtaggttt tgcttatcaa tctcttgaac
     4561 gtttaacttt ttttgacaca tctcactcta agacagttat cgcgcataga ggacttgtat
     4621 cagcaggtgt agaaaattct ctggaagccc ttgaaggtgc taagaaagca ggaagtgatt
     4681 atgtagaact ggatctaatc ttgactaagg ataatcactt tgtggtgtct catgataatc
     4741 gattgaagcg tttagctgga gtaaataaga cgattcgcaa cttaacctta aaagaagttg
     4801 aacatctaac gagtcatcaa ggacattttt cagggcgttt tgtttctttt gacacttttt
     4861 atcaaaaggc taagaagttg aatatgccat tacttattga actcaagcca attggtacag
     4921 aacctggaaa ttatgtcgat ttgtttttag aaacttatca tcgacttggt ataagcaaag
     4981 ataataaagt catgtcttta gatttagaag taatagaagc tatcaagaaa aaaaatccat
     5041 caattacgac tggttatatc ataccaattc aatttggctt ttttggagat gaatttgttg
     5101 atttctatgt cattgaagac ttttcttatc ggtcttattt gtcgtcccaa gctttttgga
     5161 ataataaaga aatttacgtt tggactatta atgatcccaa gcgcatagag cattatctcc
     5221 taaagcctat tcagggaatt attacagatc aaccagcttt aactaatcaa ttgattaaag
     5281 acttaaaaca agataattct tattttagtc gattagtcag aattattagt agtctttatt
     5341 aaaggtttaa agaaagtgtc aagaaaaaaa cttgacactt tctttaaact gagttatact
     5401 gtttaacata ggaatttagc gtagctgtat ttcttataca ttactaaaag aaaagagact
     5461 atttaaaaat ggcagtaaaa atccgtttaa ctcgtatggg ttctaagaaa aaacctttct
     5521 accgtattaa tgttgcagat tcacgtgcac cacgtgatgg tcgttttatc gaaacagttg
     5581 gaacatacaa cccattagtg gctgaaaatc aaattactat taaagaggat cgtgttcttg
     5641 agtggttatc taaaggagcg cagccttctg ataccgttcg taacatcttg tctaaagctg
     5701 gtgttatggc taagttccac gaccaaaaat tctctaaata ataacagcct atggatacca
     5761 ttgaaaatct tattatcgct attgtgaaac ctttgatttc acaaccagat aatcttacca
     5821 ttaaaattga agatactcct gactttttag agtatcatct tgacttggat gctcaagaca
     5881 tcggtcgtgt tatcggcaaa aaaggtcgaa ctattactgc gataagatcg attgtctact
     5941 cggttccaac actaggaaaa aaagttcgct tagttattga tgagaaataa tcactaactt
     6001 gagatggatt gaaaagcttg ttgacttaaa gtcaacaagc tttttgaata gcaaaaatct
     6061 taatatttct gaaatgaaaa gattagattt ttctgtcttt aactaaaaag aagttaaaga
     6121 atggcttagc tgattgcctt tataagagat tatactggtt tttgatagct gctgtcatgt
     6181 aaaatagcta caattcatct tacgagattg accttagcat tgccagtgca gtctttattt
     6241 tctaacctgc tgtttgttgt atgtttatag tttcctaaaa aatgctctct cagttttttg
     6301 ggggacattc ctaaaattaa gtatacttaa ttttagg
//
LOCUS       DATXIG010000073         6030 bp    DNA     linear   BCT 28-DEC-2023
DEFINITION  TPA_asm: Streptococcus pyogenes strain MGAS11113
            SAMN08555799-rid19682583.denovo.073, whole genome shotgun sequence.
ACCESSION   DATXIG010000073 DATXIG010000000
VERSION     DATXIG010000073.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN08555799
            Sequence Read Archive: SRR6740495
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pyogenes
  ORGANISM  Streptococcus pyogenes
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 6030)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6030)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-OCT-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-OCT-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001918058.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001918058.2
            Genome Coverage       :: 368x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/12/2023 11:23:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 1,806
            CDSs (total)                      :: 1,769
            Genes (coding)                    :: 1,722
            CDSs (with protein)               :: 1,722
            Genes (RNA)                       :: 37
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1, 1 (5S, 23S)
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 47
            CDSs (without protein)            :: 47
            Pseudo Genes (ambiguous residues) :: 0 of 47
            Pseudo Genes (frameshifted)       :: 30 of 47
            Pseudo Genes (incomplete)         :: 20 of 47
            Pseudo Genes (internal stop)      :: 9 of 47
            Pseudo Genes (multiple problems)  :: 11 of 47
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6030
                     /organism="Streptococcus pyogenes"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN08555799-rid19682583.denovo.073"
                     /strain="MGAS11113"
                     /serotype="Emm28"
                     /isolation_source="normally sterile site"
                     /host="Homo sapiens"
                     /db_xref="taxon:1314"
                     /country="Finland"
                     /collection_date="2002"
                     /collected_by="National Public Health Laboratory"
     gene            83..4189
                     /gene="cas9"
                     /locus_tag="VC122_001537"
     CDS             83..4189
                     /gene="cas9"
                     /locus_tag="VC122_001537"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010922251.1"
                     /note="Cas9, originally named Csn1, is the large,
                     multifunctional signature protein of type II CRISPR/Cas
                     systems. It is well known even to general audiences
                     because its RNA-guided endonuclease activity has made it a
                     popular tool for custom editing of eukaryotic genomes;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II CRISPR RNA-guided endonuclease Cas9"
                     /protein_id="HEP5641453.1"
                     /translation="MDKKYSIGLDIGTNSVGWAVITDDYKVPSKKFKVLGNTDRHSIK
                     KNLIGALLFDSGETAEATRLKRTARRRYTRRKNRICYLQEIFSNEMAKVDDSFFHRLE
                     ESFLVEEDKKHERHPIFGNIVDEVAYHEKYPTIYHLRKKLVDSTDKADLRLIYLALAH
                     MIKFRGHFLIEGDLNPDNSDVDKLFIQLVQTYNQLFEENPINASGVDAKAILSARLSK
                     SRRLENLIAQLPGEKKNGLFGNLIALSLGLTPNFKSNFDLAEDAKLQLSKDTYDDDLD
                     NLLAQIGDQYADLFLAAKNLSDAILLSDILRLNSEITKAPLSASMIKRYDEHHQDLTL
                     LKALVRQQLPEKYKEIFFDQSKNGYAGYIDGGASQEEFYKFIKPILEKMDGTEELLAK
                     LNREDLLRKQRTFDNGSIPHQIHLGELHAILRRQEDFYPFLKDNREKIEKILTFRIPY
                     YVGPLARGNSRFAWMTRKSEETITPWNFEEVVDKGASAQSFIERMTNFDKNLPNEKVL
                     PKHSLLYEYFTVYNELTKVKYVTEGMRKPAFLSGEQKKAIVDLLFKTNRKVTVKQLKE
                     DYFKKIECFDSVEISGVEDRFNASLGTYHDLLKIIKDKDFLDNEENEDILEDIVLTLT
                     LFEDREMIEERLKTYAHLFDDKVMKQLKRRRYTGWGRLSRKLINGIRDKQSGKTILDF
                     LKSDGFANRNFMQLIHDDSLTFKEDIQKAQVSGQGDSLHEHIANLAGSPAIKKGILQT
                     VKVVDELVKVMGRHKPENIVIEMARENQTTQKGQKNSRERMKRIEEGIKELGSQILKE
                     HPVENTQLQNEKLYLYYLQNGRDMYVDQELDINRLSDYDVDHIVPQSFIKDDSIDNKV
                     LTRSDKNRGKSNNVPSEEVVKKMKNYWRQLLNAKLITQRKFDNLTKAERGGLSELDKA
                     GFIKRQLVETRQITKHVAQILDSRMNTKYDENDKLIREVKVITLKSKLVSDFRKDFQF
                     YKVREINNYHHAHDAYLNAVVGTALIKKYPKLESEFVYGDYKVYDVRKMIAKSEQEIG
                     KATAKYFFYSNIMNFFKTEITLANGEIRKRPLIETNGETGEIVWDKGRDFATVRKVLS
                     MPQVNIVKKTEVQTGGFSKESILPKRNSDKLIARKKDWDPKKYGGFDSPTVAYSVLVV
                     AKVEKGKSKKLKSVKELLGITIMERSSFEKNPIDFLEAKGYKEVRKDLIVKLPKYSLF
                     ELENGRKRMLASAGELQKGNELALPSKYVNFLYLASHYEKLKGSPEDNEQKQLFVEQH
                     KHYLDEIIEQISEFSKRVILADANLDKVLSAYNKHRDKPIREQAENIIHLFTLTNLGA
                     PAAFKYFDTTIDRKRYTSTKEVLDATFIHQSITGLYETRIDLSQLGGD"
     gene            4189..5058
                     /gene="cas1"
                     /locus_tag="VC122_001538"
     CDS             4189..5058
                     /gene="cas1"
                     /locus_tag="VC122_001538"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000929484.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II CRISPR-associated endonuclease Cas1"
                     /protein_id="HEP5641454.1"
                     /translation="MAGWRTVVVNTHSKLSYKNNHLIFKDAYKTELIHLSEIDILLLE
                     TTDIVLSTMLVKRLVDENVLVIFCDDKRLPTAMLMPFYGRHDSSLQLGKQMSWSETVK
                     SQVWTTIIAQKILNQSCYLGACSYFEKSQSIMDLYHGLENFDPSNREGHAARIYFNTL
                     FGNDFSRDLEHPINAGLDYGYTLLLSMFAREVVVSGCMTQFGLKHANQFNQFNFASDI
                     MEPFRPLVDKIVYENRNQPFPKIKRELFTLFSDTFSYNGKEMYLTNIISDYTKKVVKA
                     LNNEGKGVPEFRI"
     gene            5055..5396
                     /gene="cas2"
                     /locus_tag="VC122_001539"
     CDS             5055..5396
                     /gene="cas2"
                     /locus_tag="VC122_001539"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019803445.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CRISPR-associated endonuclease Cas2"
                     /protein_id="HEP5641455.1"
                     /translation="MSYRYMRMILMFDMPTDTAEERKAYRKFRKFLLSEGFIMHQFSI
                     YSKLLLNNTANNAMIGRLREHNPNKGNITLLTVTEKQFARMIYLHGERNNCIANSDER
                     LVFLGEAFDES"
     gene            5386..>6030
                     /gene="csn2"
                     /locus_tag="VC122_001540"
     CDS             5386..>6030
                     /gene="csn2"
                     /locus_tag="VC122_001540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002989949.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II-A CRISPR-associated protein Csn2"
                     /protein_id="HEP5641456.1"
                     /translation="MNLNFSLLDEPIPLRGGTILVLEDVCVFSKIVQYCYKYEEDSEL
                     KFFDRKMKTIKESEIMLVTDILGFDVNSSTILKLIHADLESQFNEKPEVKSMIDKLVA
                     TITELIVFECLENELDLEYDEITILELIKSLGVKVETQSDTIFEKCLEILQIFKYLTK
                     KKLLIFVNSGAFLTKDEVASLQEYISLTNLTVLFLEPRELYDFPQYILDEDYFLI"
BASE COUNT         2087 a          884 c         1198 g         1861 t
ORIGIN      
        1 tttcggaggt cattaaaact attagtgaaa tcatcaaact cattatggat ttaatttaaa
       61 ctttttattt taggaggcaa aaatggataa gaaatactca ataggcttag atatcggcac
      121 aaatagcgtc ggatgggcgg tgatcactga tgattataag gttccgtcta aaaagttcaa
      181 ggttctggga aatacagacc gccacagtat caaaaaaaat cttatagggg ctcttttatt
      241 tgacagtgga gagacagcgg aagcgactcg tctcaaacgg acagctcgta gaaggtatac
      301 acgtcggaag aatcgtattt gttatctaca ggagattttt tcaaatgaga tggcgaaagt
      361 agatgatagt ttctttcatc gacttgaaga gtcttttttg gtggaagaag acaagaagca
      421 tgaacgtcat cctatttttg gaaatatagt agatgaagtt gcttatcatg agaaatatcc
      481 aactatctat catctgcgaa aaaaattggt agattctact gataaagcgg atttgcgctt
      541 aatctatttg gccttagcgc atatgattaa gtttcgtggt cattttttga ttgagggaga
      601 tttaaatcct gataatagtg atgtggacaa actatttatc cagttggtac aaacctacaa
      661 tcaattattt gaagaaaacc ctattaacgc aagtggagta gatgctaaag cgattctttc
      721 tgcacgattg agtaaatcaa gacgattaga aaatctcatt gctcagctcc ccggtgagaa
      781 gaaaaatggc ttatttggga atctcattgc tttgtcattg ggtttgaccc ctaattttaa
      841 atcaaatttt gatttggcag aagatgctaa attacagctt tcaaaagata cttacgatga
      901 tgatttagat aatttattgg cgcaaattgg agatcaatat gctgatttgt ttttggcagc
      961 taagaattta tcagatgcta ttttactttc agatatccta agattaaata gtgaaataac
     1021 taaggctccc ctatcagctt caatgattaa acgctacgat gaacatcatc aagacttgac
     1081 tcttttaaaa gctttagttc gacaacaact tccagaaaag tataaagaaa tcttttttga
     1141 tcaatcaaaa aacggatatg caggttatat tgatggggga gctagccaag aagaatttta
     1201 taaatttatc aaaccaattt tagaaaaaat ggatggtact gaggaattat tggcgaaact
     1261 aaatcgtgaa gatttgctgc gcaagcaacg gacctttgac aacggctcta ttccccatca
     1321 aattcacttg ggtgagctgc atgctattct gagaagacaa gaagactttt atccattttt
     1381 aaaagacaat cgtgagaaga ttgaaaaaat cttgactttt cgaattcctt attatgttgg
     1441 tccattggcg cgtggcaata gtcgttttgc atggatgact cggaagtctg aagaaacaat
     1501 taccccatgg aattttgaag aagttgtcga taaaggtgct tcagctcaat catttattga
     1561 acgcatgaca aactttgata aaaatcttcc aaatgaaaaa gtactaccaa aacatagttt
     1621 gctttatgag tattttacgg tttataacga attgacaaag gtcaaatatg ttactgaggg
     1681 aatgcgaaaa ccagcatttc tttcaggtga acagaagaaa gccattgttg atttactctt
     1741 caaaacaaat cgaaaagtaa ccgttaagca attaaaagaa gattatttca aaaaaataga
     1801 atgttttgat agtgttgaaa tttcaggagt tgaagataga tttaatgctt cattaggtac
     1861 ctaccatgat ttgctaaaaa ttattaaaga taaagatttt ttggataatg aagaaaatga
     1921 agatatctta gaggatattg ttttaacatt gaccttattt gaagataggg agatgattga
     1981 ggaaagactt aaaacatatg ctcacctctt tgatgataag gtgatgaaac agcttaaacg
     2041 tcgccgttat actggttggg gacgtttgtc tcgaaaattg attaatggta ttagggataa
     2101 gcaatctggc aaaacaatat tagatttttt gaaatcagat ggttttgcca atcgcaattt
     2161 tatgcagctg atccatgatg atagtttgac atttaaagaa gacattcaaa aagcacaagt
     2221 gtctggacaa ggcgatagtt tacatgaaca tattgcaaat ttagctggta gccctgctat
     2281 taaaaaaggt attttacaga ctgtaaaagt tgttgatgaa ttggtcaaag taatggggcg
     2341 gcataagcca gaaaatatcg ttattgaaat ggcacgtgaa aatcagacaa ctcaaaaggg
     2401 ccagaaaaat tcgcgtgagc gtatgaaacg tattgaagaa ggtatcaaag aattaggaag
     2461 tcagattctt aaagagcatc ctgttgaaaa tactcaattg caaaatgaaa agctctatct
     2521 ctattatctc caaaatggaa gagacatgta tgtggaccaa gaattagata ttaatcgttt
     2581 aagtgattat gatgtcgatc acattgttcc acaaagtttc attaaagacg attcaataga
     2641 caataaggtc ttaacgcgtt ctgataaaaa tcgtggtaaa tcgaataacg ttccaagtga
     2701 agaagtagtc aaaaagatga aaaactattg gagacaactt ctaaacgcca agttaatcac
     2761 tcaacgtaag tttgataatt taacgaaagc tgaacgtgga ggtttgagtg aacttgataa
     2821 agctggtttt atcaaacgcc aattggttga aactcgccaa atcactaagc atgtggcaca
     2881 aattttggat agtcgcatga atactaaata cgatgaaaat gataaactta ttcgagaggt
     2941 taaagtgatt accttaaaat ctaaattagt ttctgacttc cgaaaagatt tccaattcta
     3001 taaagtacgt gagattaaca attaccatca tgcccatgat gcgtatctta atgccgtcgt
     3061 tggaactgct ttgattaaga aatatccaaa acttgaatcg gagtttgtct atggtgatta
     3121 taaagtttat gatgttcgta aaatgattgc taagtctgag caggaaatag gcaaagcaac
     3181 cgcaaaatat ttcttttact ctaatatcat gaacttcttc aaaacagaaa ttacacttgc
     3241 aaatggagag attcgcaaac gccctctaat cgaaactaat ggggaaactg gagaaattgt
     3301 ctgggataaa gggcgagatt ttgccacagt gcgcaaagta ttgtccatgc cccaagtcaa
     3361 tattgtcaag aaaacagaag tacagacagg cggattctcc aaggagtcaa ttttaccaaa
     3421 aagaaattcg gacaagctta ttgctcgtaa aaaagactgg gatccaaaaa aatatggtgg
     3481 ttttgatagt ccaacggtag cttattcagt cctagtggtt gctaaggtgg aaaaagggaa
     3541 atcgaagaag ttaaaatccg ttaaagagtt actagggatc acaattatgg aaagaagttc
     3601 ctttgaaaaa aatccgattg actttttaga agctaaagga tataaggaag ttagaaaaga
     3661 cttaatcgtt aaactaccta aatatagtct ttttgagtta gaaaacggtc gtaaacggat
     3721 gctggctagt gccggagaat tacaaaaagg aaatgagctg gctctgccaa gcaaatatgt
     3781 gaatttttta tatttagcta gtcattatga aaagttgaag ggtagtccag aagataacga
     3841 acaaaaacaa ttgtttgtgg agcagcataa gcattattta gatgagatta ttgagcaaat
     3901 cagtgaattt tctaagcgtg ttattttagc agatgccaat ttagataaag ttcttagtgc
     3961 atataacaaa catagagaca aaccaatacg tgaacaagca gaaaatatta ttcatttatt
     4021 tacgttgacg aatcttggag ctcccgctgc ttttaaatat tttgatacaa caattgatcg
     4081 taaacgatat acgtctacaa aagaagtttt agatgccact tttatccatc aatccatcac
     4141 tggtctttat gaaacacgca ttgatttgag tcagctagga ggtgactgat ggctggttgg
     4201 cgtactgttg tggtaaatac ccactcgaaa ttatcctata agaataatca tctgattttt
     4261 aaggatgcct ataaaacgga gctgatccat ttatcagaaa ttgatatttt gttattagaa
     4321 acgaccgata ttgtcttgtc cactatgctg gtaaaacggc tagtggatga gaatgtcctt
     4381 gtcatattct gtgatgataa acgattacca acagctatgc tgatgccttt ttatggtcgt
     4441 catgattcga gtttacagct tgggaaacaa atgtcctggt cagaaacagt caaatcgcag
     4501 gtttggacga cgattattgc tcaaaagatt ttgaatcaat cttgctatct aggagcatgc
     4561 tcctattttg aaaaatccca atctattatg gatttatatc atggtttgga aaattttgat
     4621 ccgagtaatc gagaagggca tgcagcgaga atttatttta atacactttt tgggaacgat
     4681 ttctcaagag atttggagca tccaatcaat gcaggtctgg attatggtta tactttatta
     4741 ttgagtatgt ttgcgcgtga agtggttgtg tctggatgta tgactcagtt tgggcttaaa
     4801 cacgctaatc agtttaatca gttcaatttt gctagcgata ttatggaacc atttaggcct
     4861 ttggtggata agattgttta tgaaaatcga aatcagcctt ttcccaaaat aaagagagag
     4921 ttatttactt tgttttcaga tacattttca tataatggta aagagatgta tctcacgaat
     4981 attattagcg attatactaa aaaagttgtc aaagctctga ataatgaagg gaaaggagtt
     5041 cctgaattta ggatatgagt tatagatata tgagaatgat acttatgttt gatatgccga
     5101 cggacaccgc tgaggaacga aaagcctatc gaaaatttcg gaaattttta cttagtgaag
     5161 ggtttatcat gcatcaattt tctatttata gtaagttgct gttgaataat acagctaaca
     5221 atgccatgat tggtcggctg agggagcata atcctaataa aggaaatatt acattactaa
     5281 cggtcacaga aaaacagttt gcacgaatga tttatttaca tggtgaaaga aataattgta
     5341 ttgcaaactc cgatgaaaga cttgtatttc ttggggaggc ttttgatgaa tcttaatttt
     5401 tccttactag atgaaccgat tccattaaga ggcggtacaa ttcttgtgct cgaagatgtc
     5461 tgtgtatttt caaaaatagt gcaatattgt tacaaatatg aggaagattc tgaacttaaa
     5521 ttttttgatc gcaagatgaa aaccatcaaa gaatcagaaa tcatgcttgt aacagatatt
     5581 ttaggatttg atgttaactc ctcaaccatt ttaaaattga ttcatgcaga tttagaatct
     5641 caatttaatg agaaacccga agtgaaatcg atgattgaca aattggttgc tacgattaca
     5701 gaactgattg tctttgaatg cttagaaaat gaattagatt tagagtatga tgaaatcaca
     5761 atcctggaat tgattaagtc cttaggagta aaagtagaaa cgcaaagtga tactattttt
     5821 gaaaaatgtc tagagatact tcaaattttc aaatatctca ctaagaaaaa gttgcttatt
     5881 tttgtcaata gcggagcttt tctaacaaag gatgaagtgg ctagtttaca agagtatata
     5941 tcattgacaa atttaacagt tctcttttta gaaccacgtg aactatatga ttttccgcag
     6001 tatattttag atgaagatta tttcttaata
//
LOCUS       DATXIG010000074         5649 bp    DNA     linear   BCT 28-DEC-2023
DEFINITION  TPA_asm: Streptococcus pyogenes strain MGAS11113
            SAMN08555799-rid19682583.denovo.074, whole genome shotgun sequence.
ACCESSION   DATXIG010000074 DATXIG010000000
VERSION     DATXIG010000074.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN08555799
            Sequence Read Archive: SRR6740495
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pyogenes
  ORGANISM  Streptococcus pyogenes
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 5649)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5649)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-OCT-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-OCT-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001918058.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001918058.2
            Genome Coverage       :: 368x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/12/2023 11:23:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 1,806
            CDSs (total)                      :: 1,769
            Genes (coding)                    :: 1,722
            CDSs (with protein)               :: 1,722
            Genes (RNA)                       :: 37
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1, 1 (5S, 23S)
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 47
            CDSs (without protein)            :: 47
            Pseudo Genes (ambiguous residues) :: 0 of 47
            Pseudo Genes (frameshifted)       :: 30 of 47
            Pseudo Genes (incomplete)         :: 20 of 47
            Pseudo Genes (internal stop)      :: 9 of 47
            Pseudo Genes (multiple problems)  :: 11 of 47
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5649
                     /organism="Streptococcus pyogenes"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN08555799-rid19682583.denovo.074"
                     /strain="MGAS11113"
                     /serotype="Emm28"
                     /isolation_source="normally sterile site"
                     /host="Homo sapiens"
                     /db_xref="taxon:1314"
                     /country="Finland"
                     /collection_date="2002"
                     /collected_by="National Public Health Laboratory"
     gene            complement(11..1246)
                     /locus_tag="VC122_001541"
     CDS             complement(11..1246)
                     /locus_tag="VC122_001541"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011284832.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cation diffusion facilitator family transporter"
                     /protein_id="HEP5641457.1"
                     /translation="MTQDPIANLKLARKGPIVSIIVYLLLSAAKLLAGYLLNASSLIA
                     DGFNNLSDIVGNVALLIGLHLASQPADANHKFGHWKIEDLSSLVTSFIMFLVGFQVLI
                     HTIKRIFSGQQVDIDPLGAIVGIVSAFVMLGVYVFNKRLSKRVKSSALVAASKDNLAD
                     AVTSIGTSIAIIAASLHLPVIDHIAAMIITFFILKTAFDIFMESSFSLSDGFDSRHLK
                     KYEKAILEIPKIVAVKSQRARTYGSNVYLDIVLEMNPDLSVYESHSITEKVEQLLSDQ
                     FSIYDIDIHVEPAMILEEEIFDNVAKKLYRYEKLILSKVPDYDHYIAKSFQLVDANGQ
                     TVNYEQFLNQEIYYPSNFNHFQIESISQKTMLVTYQLNGNQHTSIWRRHESWSLLFHQ
                     ITPIAKRQLHHTHYRIVKM"
     gene            complement(1366..2688)
                     /locus_tag="VC122_001542"
     CDS             complement(1366..2688)
                     /locus_tag="VC122_001542"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014407622.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sodium:alanine symporter family protein"
                     /protein_id="HEP5641458.1"
                     /translation="MIALVKLIDNLVWGPPLLILLVGTGIYLTSRLGLIQILKLPRAF
                     KLIFSDDEGHGDISSFAALATALAATVGTGNIVGVATAIKSGGPGALFWMWVAAFFGM
                     ATKYAEGVLAIKYRTKDANGHISGGPMYYIVNGMGTKWKPLAILFAGSGILVALFGIG
                     TFAQVNSITSSLGHSFGLSPQMVSIVLAIFVAAIIFGGIHSISKVAEKVVPFMAIFYI
                     LSSLAVIFSHYQQLLPVIRLVFQSAFTPTAAIGGFAGSLMKDAIQKGIARGVFSNESG
                     LGSAPIAAAAAKTNESVEQGLISMTGTFIDTIIICTLTGLSILVTGQWTGQLEGAPLT
                     QSAFATVFGNLGTFGLTFSLVLFAFTTILGWSYYGERCFEFLFGITHLTYFRIVFILM
                     VGLGGFLKLELIWVLADIVNGLMALPNLIALLALSPVVILETKHYFIK"
     regulatory      complement(2816..2902)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00504"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="glycine riboswitch; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /bound_moiety="glycine"
                     /db_xref="RFAM:RF00504"
     gene            3217..5535
                     /gene="pcrA"
                     /locus_tag="VC122_001543"
     CDS             3217..5535
                     /gene="pcrA"
                     /locus_tag="VC122_001543"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011017827.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA helicase PcrA"
                     /protein_id="HEP5641459.1"
                     /translation="MNPLLNGMNDRQAQAVQTTEGPLLIMAGAGSGKTRVLTHRIAYL
                     IDEKFVNPWNILAITFTNKAAREMKERALSLNPATKDTLIATFHSMCVRILRREADHI
                     GYNRNFTIVDPGEQRTLMKRILKQLNIDPKKWNERSILGTISNAKNDLLDEKGYEAQA
                     ADMYSQIVARCYKAYQEELRRSEALDFDDLIMMTLRLFDANPDVLAYYQQRYQYIHVD
                     EYQDTNHAQYQLIKLLASRFKNICVVGDADQSIYGWRGADMQNILDFEKDYPDAKVVL
                     LEENYRSTKKILQAANDVINNNRNRRPKKLWTQNADGEQLVYYRANDERDEAVFVAST
                     ISNMSQELGKNFKDFAVLYRTNAQSRTIEEALLKSNIPYTMVGGTKFYSRKEIRDLIA
                     YLTIVANPADNISFERIVNEPKRGVGPGTLDKLRQFAYESDQSLLEAASNLLMSPLKG
                     KAAQAIMDLANILGQLRQDLDQMSITDLAEALLEKTGYLDSLRLQNTLESQARIENIE
                     EFLSVTKNFDESSASQEEDETGVDRLGRFLNDLALIADTDDSQAEAAEVTLMTLHAAK
                     GLEFPVVFLIGMEEGVFPLSRASEDPDELEEERRLAYVGITRAEEVLFMTNANTRTLF
                     GKSSYNRPTRFLKEISEELLSYKGLARPAQSSFGVRFSTETHTQFGQGMSLSEALQAR
                     KAQAQVRQSAQPMQAHTIPSASTSSVLPFGSNSSVEEVTWQIGDIAHHKKWGDGTVLE
                     VSGSGKTMELKIKFPEVGLKKLLASVAPIEKK"
BASE COUNT         1875 a         1088 c         1140 g         1546 t
ORIGIN      
        1 agtaatgttt ttacattttt acaatacgat agtgtgtgtg atgtaattgt ctcttagcga
       61 taggggtaat ttggtggaag agtaaggacc aagattcatg acgcctccaa atactggtat
      121 gttgattgcc atttaattgg taagttacca acatcgtttt ttgactaatg gattcaatct
      181 gaaaatggtt gaagttactt ggataataaa tttcttggtt caaaaattgt tcatagttaa
      241 ctgtttggcc attcgcatca accagttgga aagacttagc aatgtagtgg tcatagtcag
      301 gaaccttact caaaattaat ttttcgtagc ggtagagctt tttggcgaca ttatcaaaaa
      361 tctcttcttc gagaatcatg gcaggttcaa cgtgaatatc aatgtcataa atagaaaatt
      421 ggtcactcaa taactgctcc actttctctg taatagagtg actttcatag actgaaagat
      481 caggattcat ttcaagtaca atatcaagat agacattgct accataggtc ctagctcgtt
      541 gagacttaac ggcgacaatt ttagggattt ctaaaatggc tttttcgtat tttttcaaat
      601 gacggctatc aaatccatca gataaactaa acgaactttc cataaagata tcaaaagctg
      661 ttttaagaat aaagaacgta atgatcatag cagctatatg atcgataact ggtaaatgca
      721 aagaagctgc tataatagca attgatgttc cgatagaagt aacagcatca gctagattat
      781 ccttagaagc agcgactaag gcactggatt ttacacgttt ggaaagacgt ttgttaaaga
      841 catagacccc taacataaca aaagctgaaa cgataccgac aatagcccca agagggtcaa
      901 tatcaacttg ctgaccacta aagatacgtt taattgtgtg aatcagtact tggaaaccta
      961 caagaaacat aataaaagaa gtgacaaggc tggataaatc ttcaattttc caatgaccaa
     1021 atttatgatt ggcatcggct ggttggctag ctaagtgaag accaataagc agggctacat
     1081 ttcccacaat atccgataaa ttgttaaatc catcagcaat aagggaactt gcatttaaca
     1141 aataaccagc taataatttg gcagcactaa gcaacaagta gacaataata ctaacgattg
     1201 ggccctttct ggctagtttt aaatttgcaa tcggatcctg tgtcataata actcctttga
     1261 tttcaacttt atattatacc ataaaaaggg gcttaagaaa aaagcctagc ttcttgaact
     1321 tggcagaaga agactaggta taactgggta gtactaacgt catgtttact taataaagta
     1381 atgcttggtt tctaaaataa caactggcga gagagctagg agggcaatca ggttaggaag
     1441 tgccattaaa ccattgacaa tatcagccaa aacccaaatc agttctaact taaggaagcc
     1501 tcctaggcct accattaaga taaagacaat acggaagtag gtgagatgtg taatgccaaa
     1561 gaggaattca aagcagcgtt ctccataata actccatcct aaaatcgttg taaaggcgaa
     1621 gagaactagg gaaaaggtta ggccaaaagt ccctaaattt ccaaacacag tggcaaaggc
     1681 agattgggtc agaggagccc cttcgagctg cccagtccat tggcctgtta ctaaaataga
     1741 taggccggtt agggtacaga taataatcgt atcaataaaa gttcctgtca tggagatcag
     1801 gccctgctcc acggattcat tggttttagc tgctgctgca gcaataggtg cagatcctag
     1861 tccagactca ttggagaaga caccgcgtgc aattcctttt tgaatggcat ccttcatgag
     1921 acttccagca aagcctccaa tagcagctgt cggagtgaag gctgattgaa agactaaacg
     1981 aataacagga agtagctgct ggtagtgact aaaaataact gctaaactag atagaatata
     2041 aaaaatagcc ataaaaggaa cgactttttc agccactttg gaaatggaat gaatgccacc
     2101 aaaaataatg gcagccacaa agatagctaa aacaatactg accatttgtg gggataagcc
     2161 aaagctatga cctaaagacg aggtgatcga gttcacttga gcaaaggttc ctattccaaa
     2221 tagggcaact aggatccctg aacccgcaaa aagaatggct aagggtttcc attttgtccc
     2281 catgccatta acaatatagt acataggacc accagaaata tgaccgttgg catcctttgt
     2341 acggtattta atcgctagta ccccttcagc gtacttagtg gccattccaa aaaaagcggc
     2401 aacccacatc caaaagagcg ctccaggacc accagacttg atagcagtgg caaccccaac
     2461 aatgttacca gttccgacag tagcggcaag ggcagttgca agagcagcaa aggatgaaat
     2521 atctccatgt ccttcgtcat ctgaaaaaat gagtttaaag gctcttggta gttttaagat
     2581 ttgaattaat cctaaacgac tggtaaggta aatccccgtc ccaaccaata aaattaagag
     2641 gggcggtccc caaacaaggt tatcaattaa tttaacgagt gctatcatct ttctctccta
     2701 aaagtcatcg aagacgccaa aagaaaaaag ctatctcttt ctttgagaca acttctgaag
     2761 caatgataaa acagcaaaca caaaaaagac taagactgtt cttaatggtg tttgtatttt
     2821 accttctgtc cttttgcctg agagattgag cagtatgcct tgccccttcg gcgcctaata
     2881 aggtctctcc agaagtccgt ccatcaaaca gtcccgttca aaaactgaac acctgagagt
     2941 ataactcctt cggcgaaaaa aattcttctc ctgcatgaca tcattcggtt tattaaatta
     3001 ggactatcat acctcaaaaa acgaatgaag tcaaccaaaa tagcaaaaaa ataaggaatg
     3061 ctttaagaaa tcgctattat ttttaaatat ctgatcctaa actagaaaaa aggaatctct
     3121 ttacttttgg ggtcttcttt gctatttttc ttggaaacat gatataattt taaggttgat
     3181 aaaagagatg tcactgaacg agattaagga gaaagcatga atcctttatt aaatggaatg
     3241 aatgatcggc aggcacaagc tgttcaaaca actgaaggcc ccttattgat tatggcgggg
     3301 gctggttctg gtaaaacaag ggtactgacc catcgtattg cctatttaat tgatgaaaaa
     3361 tttgtcaatc cttggaatat tttagctatt acctttacta ataaggctgc gcgtgaaatg
     3421 aaagaaagag ccttgtcctt aaatccagct actaaagata ccttaattgc tacctttcac
     3481 tccatgtgtg tacggattct gcgtcgtgag gctgatcaca ttggttacaa ccgaaacttc
     3541 acaatcgtgg atccaggtga gcaacgaacg ttgatgaagc gcattttaaa acaattaaat
     3601 atagacccta aaaagtggaa tgaacgttct attttaggaa ctatttcaaa cgctaaaaat
     3661 gatttgcttg atgaaaaggg atacgaggca caggctgctg atatgtatag tcaaatcgta
     3721 gcgaggtgtt acaaagctta tcaggaagaa ttacgacgta gcgaagcact tgactttgat
     3781 gatttaatca tgatgacgct acgcctgttt gacgctaatc ctgacgtgtt agcttattac
     3841 cagcagcgtt accaatacat tcatgtggat gagtaccaag ataccaacca tgctcaatat
     3901 cagctaatta aattgctagc ttctcgcttt aaaaatattt gcgttgttgg agacgctgat
     3961 cagtctatct atggttggcg gggagctgac atgcaaaata ttcttgattt tgaaaaagat
     4021 tacccagatg ctaaggtagt gcttttagaa gaaaactatc gctctaccaa gaaaattctg
     4081 caagcagcta acgacgtgat taacaataac cgtaatcgcc gtcctaagaa actttggaca
     4141 caaaatgcgg atggtgaaca actggtttat taccgggcaa atgatgagcg agatgaggca
     4201 gtatttgtgg cctcaaccat tagcaatatg agtcaagagc ttggaaaaaa cttcaaagat
     4261 ttcgctgttt tataccgtac caatgcccaa tctcggacca ttgaagaagc actcttaaaa
     4321 tccaatattc cttacaccat ggttggaggc accaagtttt acagccgtaa agaaattcgg
     4381 gatttgattg cctatttaac cattgttgct aaccctgctg ataatatttc ttttgagcgg
     4441 attgtgaatg agcctaaacg aggtgttggt ccaggaacgc ttgataaact gcgtcagttt
     4501 gcctatgaga gtgaccagtc tttattagaa gcagcttcaa atctattgat gtcccccttg
     4561 aaaggtaaag cagcacaagc tatcatggac ttggccaata ttctcggtca gctacgtcaa
     4621 gacctagatc agatgagtat caccgactta gcagaagctt tgttagaaaa aacaggttat
     4681 ttagatagct tacgcctgca aaatacattg gaaagccaag cccgtatcga aaatatcgaa
     4741 gaattcttat ctgttaccaa aaattttgac gagtcaagtg cctctcaaga agaagacgaa
     4801 accggtgttg atcgcttggg acgtttttta aacgatttag cactgattgc tgatacagat
     4861 gattcccaag cagaagctgc tgaagtaacc ttaatgaccc tacatgctgc caaaggcttg
     4921 gagttccctg ttgttttctt aattggtatg gaagaaggtg tcttcccctt atcacgtgcc
     4981 tctgaagatc cagatgaatt ggaagaagaa agacgtttag cttatgtcgg aatcacaaga
     5041 gctgaagaag ttctctttat gaccaatgcc aacaccagaa ccctctttgg taaaagtagt
     5101 tataaccgtc caacacgctt cttgaaagaa atttcagagg aactactctc ttataaagga
     5161 ttggcaagac cagcacaatc ttcatttggt gtgcgttttt ctactgaaac acacacacag
     5221 tttggacaag ggatgagcct atcagaagcc ttacaagctc gaaaggccca ggctcaagta
     5281 agacaatcag cacagccaat gcaggcacat acgataccat cagcttcaac tagctcagtt
     5341 ttaccgtttg gttctaatag ctctgtagaa gaggtcactt ggcagattgg cgatatcgct
     5401 caccataaaa aatggggaga cgggactgtg cttgaggttt caggctccgg aaaaacgatg
     5461 gaattgaaaa tcaaatttcc agaagttggt ctaaagaaat tactagcaag tgttgctcct
     5521 attgagaaaa aataagaaaa aaatgagctt cctactatat aagtgagtag gaagctcatt
     5581 tttttagatt tctgtatcat ataaaagtta aaaaatttta agttttagga gcatatcagt
     5641 tgcatttta
//
LOCUS       DATXIG010000075         5534 bp    DNA     linear   BCT 28-DEC-2023
DEFINITION  TPA_asm: Streptococcus pyogenes strain MGAS11113
            SAMN08555799-rid19682583.denovo.075, whole genome shotgun sequence.
ACCESSION   DATXIG010000075 DATXIG010000000
VERSION     DATXIG010000075.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN08555799
            Sequence Read Archive: SRR6740495
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pyogenes
  ORGANISM  Streptococcus pyogenes
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 5534)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5534)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-OCT-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-OCT-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001918058.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001918058.2
            Genome Coverage       :: 368x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/12/2023 11:23:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 1,806
            CDSs (total)                      :: 1,769
            Genes (coding)                    :: 1,722
            CDSs (with protein)               :: 1,722
            Genes (RNA)                       :: 37
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1, 1 (5S, 23S)
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 47
            CDSs (without protein)            :: 47
            Pseudo Genes (ambiguous residues) :: 0 of 47
            Pseudo Genes (frameshifted)       :: 30 of 47
            Pseudo Genes (incomplete)         :: 20 of 47
            Pseudo Genes (internal stop)      :: 9 of 47
            Pseudo Genes (multiple problems)  :: 11 of 47
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5534
                     /organism="Streptococcus pyogenes"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN08555799-rid19682583.denovo.075"
                     /strain="MGAS11113"
                     /serotype="Emm28"
                     /isolation_source="normally sterile site"
                     /host="Homo sapiens"
                     /db_xref="taxon:1314"
                     /country="Finland"
                     /collection_date="2002"
                     /collected_by="National Public Health Laboratory"
     gene            complement(48..1244)
                     /locus_tag="VC122_001544"
     CDS             complement(48..1244)
                     /locus_tag="VC122_001544"
                     /EC_number="2.5.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011284923.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methionine adenosyltransferase"
                     /protein_id="HEP5641460.1"
                     /translation="MSERKLFTSESVSEGHPDKIADQISDAILDAILAEDPEAHVAAE
                     TCVYTGSVHVFGEISTKAYIDINRVVRDTIAEIGYTEAEYGFSAESVGVHPSLVEQSG
                     DIAQGVNEALESREGDTDDLSHIGAGDQGLMFGFAINETPELMPLPISLSHQLVRRLA
                     ELRKSGEISYLRPDAKSQVTVEYDEHDKPVRVDTVVISTQHDPEATNDQIRQDVIEKV
                     IKAVIPADYLDDDTKFFINPTGRFVIGGPQGDSGLTGRKIIVDTYGGYSRHGGGAFSG
                     KDATKVDRSASYAARYIAKNLVAAGLATKAEVQLAYAIGVAQPVSVRVDTFGTSTVPE
                     AVLEAAVRQVFDLRPAGIIQMLDLKRPIYKQTAAYGHMGRTDIDLPWERLNKVDALVE
                     AVKTVL"
     gene            complement(1780..4158)
                     /locus_tag="VC122_001545"
     CDS             complement(1780..4158)
                     /locus_tag="VC122_001545"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010922411.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="leucine-rich repeat domain-containing protein"
                     /protein_id="HEP5641461.1"
                     /translation="MKTKKVIILVGLLLSSQLTLISCQSRGNGTYPIKTKQSRKGMTS
                     NKIKPIKKSKKTNKTHKGMAGVDFPTDDGFILTKDSKILSKTDQGIVVDHDGHSHFIF
                     YADLKGSPFEYLIPKGASLAKPAVAQQAASQGTSKVADPHHHYEFNPADIVAEDALGY
                     TVRHDDHFHYILKSSLSGQTQAQAKQVATRLPQTSSPVSTATANGIPGLHFPTSDGFQ
                     FNGQGIVGVTKDSILVDHDGHLHPISFADLRQGGWAYVADQYDPAKKAEKPAETPHTP
                     ELSEREKEYQEKLAYLAEKLGIDPSTIKRVETQDGKLGLEYPHHDHAHVLMLSDIEIG
                     KDIPEPHAIEHARELEKHKVGMDTLRALGFDEEVILDIVRTHDAPTSFPSNEKDPNKM
                     KEWLATVIKLDLGSRKDPLQRKGLSLLPNLETLGIGFTPIKDISPVLQFKKLKRLLMT
                     KTGVTDYRFLDNMPQLEGIDISQNNLKDISFLSKYKNLTLVAAADNGIEDIRPLGQLP
                     NLKFLVLSNNKISDLSPLASLHQLQELHIDNNQITDLSPVSHKESLTVVDLSRNADVD
                     LATLQAPNLETLMVNDTKVSHLDFLKNNPNLSSLSINRAQLQSLEGIEASSVIVRVEA
                     EGNQIKSLVLKDKQGSLTFLDVTGNQLTSLEGVNNFTALDILSVSKNQLTNVNLSKPN
                     KTVTNIDISHNNIPLADLKLNEQHIPEAIAKNFPAVYEGSMVGNGTAEEKAAMATKAK
                     ESAQEASESHDYNHNHAYEDEEGHAHEHRDKDDHDHEHEDENEAKDEQNHAD"
     gene            4362..5303
                     /locus_tag="VC122_001546"
     CDS             4362..5303
                     /locus_tag="VC122_001546"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015057796.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional biotin--[acetyl-CoA-carboxylase]
                     synthetase/biotin operon repressor"
                     /protein_id="HEP5641462.1"
                     /translation="MKTSEKIYQLLSQTDDFVSGEYLADQLSISRTSVWKSIKSLENQ
                     GIQIDSLKHKGYRMVQGDILLPKTISQGLGMPVTYTPHSQSTQLDAKQGIEAHNSAPR
                     LYLAPSQEAAKGRLDRQFFSASTGGIYMSMYLKPNVPYADMPPYTMMVASSIVKAISR
                     LTGIDTEIKWVNDIYLGNHKVAGILTEAITSVETGLITDVIIGVGLNFFVTDFPEAIA
                     QKAGSLFTEKPTITRNDLIIDIWKLFLSIPVKDHVKVYKEKSLVLNKQVTFTENSQEK
                     RAIAIDLTDQGHLIVQFENGDFQTLRSGEISLSSWEN"
     gene            complement(5278..5481)
                     /locus_tag="VC122_001547"
     CDS             complement(5278..5481)
                     /locus_tag="VC122_001547"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012560785.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3272 domain-containing protein"
                     /protein_id="HEP5641463.1"
                     /translation="MTIRQFLFMAFVCAFETYFFNDLLLSGNYLFALFWGLLLFRDLR
                     RVHTINQLTKTILKTANSPKKKD"
BASE COUNT         1621 a         1152 c         1015 g         1746 t
ORIGIN      
        1 agagtaagat tttttcactc actctacttt tttaataaag ttgtcgttta taatactgtt
       61 tttacagctt ctactaaagc atcaacctta tttaaacgct cccatggcaa atcaatatct
      121 gttctcccca tgtgaccata ggcagctgtt tgtttgtaaa tcgggcgttt taaatcaagc
      181 atttgaataa tgcctgctgg ccgtaaatca aagacttgac gaacagcagc ttctaaaaca
      241 gcttcaggaa ccgttgaggt tccaaatgta tccacgcgaa cagacacagg ctgagccacc
      301 ccaatcgcat aagctagttg gacttcagct ttcgtcgcaa gacctgctgc cacaaggttt
      361 ttagcaatat aacgagcagc ataagaagcc gaacggtcca cttttgtcgc atcttttcct
      421 gaaaaagcac cgccaccatg acgtgaatag cctccatagg tatccacaat aatcttacgt
      481 ccggtcaatc cagagtctcc ttgcggacct ccaataacaa aacgtcctgt tggattaatg
      541 aagaactttg tgtcatcatc taaataatct gctggaatta cagccttgat cactttttca
      601 atcacatcct gacgaatttg gtcattagta gcttctggat catgctgagt agaaataacg
      661 actgtatcca cacgaacagg tttatcgtgt tcatcgtact caactgtcac ttgagattta
      721 gcatctggac gtagataact aatctcacct gacttacgta attccgccaa gcgacgcact
      781 aattggtgag ataaggaaat tggtaaaggc atcaattctg gtgtttcatt gatggcaaaa
      841 ccaaacatga gcccttggtc acccgctcca atatggctaa ggtcatcagt atcaccttct
      901 ctgctttcga gagcttcatt aaccccctga gcgatatctc cagattgttc tacaagtgat
      961 ggatgtactc ccactgattc tgctgaaaag ccatattctg cttctgtata accaatttct
     1021 gcaatggtgt cgcggaccac acggttaata tcaatgtatg cttttgttga gatttctcca
     1081 aaaacatgaa cagaacctgt gtaaacacaa gtttctgcag ccacatgggc ttctggatct
     1141 tctgctaaaa tggcatctaa aatagcatct gaaatttggt ctgcaatctt atctggatgc
     1201 ccttcagata cggactcaga cgtgaaaagt ttacgttctg acataaaaat gtccccccta
     1261 atatgttgat tcttcctttt tcagtcacat tgaccacaaa gcaagaagtt gcctacccct
     1321 ttcttcctag ctgattgagc aagtcaagtg gccaaaacca acttagctaa tcagttacgg
     1381 ttttactagt aaggcaacta gctagtccac ctagtggcta gcgtccatta accagacaaa
     1441 gaatgtctga caatagcgtt gagttacaaa gaatccggtt tccttgttga gaggatgtca
     1501 ccacaaaata agccttagac gaagtaattt agctaaagag agaacgactg ttccccaaat
     1561 gaagtcaaca acgtctcaaa acatttcgtt caacagaccg tcttatcggg actcaataac
     1621 aatctattat aacatgtttt agccatttag gtgccattct tttcactcat tatcctattt
     1681 atttcaaaaa aacctcaagg ttattcttga ggttttcttg atagtcaaca atggtgtcat
     1741 taagacgatt cagttggatc gggttattct tagattagtt tagtcagcat ggttttgctc
     1801 atctttagct tcattttcat cctcatgttc atggtcgtga tcatctttgt ctctgtgctc
     1861 gtgagcatga ccttcttcat cttcataggc atgattatgg ttgtagtcat gtgattccga
     1921 tgcttcttga gcactttctt tcgccttagt agccatagct gctttttctt cagctgttcc
     1981 attacctacc atagaacctt cgtaaaccgc tgggaagttt ttcgcaatgg cttctggaat
     2041 atgttgctcg ttcaatttaa ggtctgccaa tgggatattg ttatgactaa tatcaatgtt
     2101 agtaactgtc ttattgggtt tagataggtt gacatttgtt aattggtttt tagacacgct
     2161 taaaatgtca agtgctgtaa aattattaac accttctaga gaagtcaact ggttgcctgt
     2221 cacatccaag aaagtaagtg acccttgctt gtctttaagc acaagcgatt taatttggtt
     2281 accttctgct tctactctga caatgacgct acttgcttca ataccttcaa gagattgcaa
     2341 ttgcgcacgg ttaatagata ggctagatag attaggatta tttttcaaga aatccaaatg
     2401 agaaaccttg gtatcattga ccattaacgt ttctaagttg ggtgcttgaa gtgttgctaa
     2461 gtcaacatca gcatttcttg ataaatcaac aaccgtcaat gattctttat gagaaacagg
     2521 gcttaaatct gtaatctgat tattatcaat gtgcaattct tgcaattgat gtaacgatgc
     2581 cagtgggctt aaatcagaaa tcttattatt actcaatacg aggaatttga gatttggtaa
     2641 ttgaccaagc ggcctaatat cttcaatacc attatcagca gccgctacta gagttaagtt
     2701 tttatatttg ctcaagaaac taatatcttt gagattgttt tgtgaaatat caatgccttc
     2761 taactgtggc atattatcca aaaatctata atcagtcacc cctgtttttg tcattagtaa
     2821 ccgtttcaat tttttaaatt gcaaaacagg tgagatatct ttgattggtg taaagccaat
     2881 tcctaaagtt tctaagttgg gtaacagtga aagtccttta cgttgcaaag gatctttacg
     2941 gctgcccaag tcaagtttga taaccgttgc taaccattct ttcatcttat tcggatcttt
     3001 ttcatttgat gggaatgagg ttggagcatc gtgagtgcga acgatatcca aaatcacttc
     3061 ttcatcaaac cctaaggcac gcaaggtatc cattccaacc ttatgttttt ccaattcacg
     3121 ggcatgctca atagcatgtg gttctggaat gtcttttccg atttcaatat cagataacat
     3181 caatacgtgt gcgtggtcat ggtgagggta ttccaaacca agtttaccgt cttgtgtttc
     3241 cacacgttta atagttgatg gatcaatccc caatttttct gccaaataag ctaatttttc
     3301 ttggtattcc ttttcacgtt cagatagctc tggtgtgtga ggggtttctg ctggcttttc
     3361 tgctttttta gcgggatcgt attgatctgc cacatatgcc cagccaccct gacgaaggtc
     3421 cgcaaaagaa ataggatgta agtgaccatc gtggtccact aaaatactgt cttttgttac
     3481 cccaacaata ccttgaccgt taaattgaaa accatctgag gttgggaaat gcaagcctgg
     3541 aataccatta gctgtagctg ttgaaacagg gctactggtt tgtggcaagc gagtagcaac
     3601 ctgtttagct tgtgcctgtg tctgacctga taagcttgac ttcaaaatat aatggaagtg
     3661 atcatcgtgg cgaaccgtgt agcctaaagc atcttcagcc acaatatccg ctgggttaaa
     3721 ttcataatgg tgatgaggat ctgctacttt agaagtccct tgactagctg cttgctgagc
     3781 aacagctggc ttagctaaac ttgctccttt tggaataagg tattcaaatg gacttccctt
     3841 taaatcggca taaaaaataa aatgcgaatg accatcatgg tcaacaacga ttccctgatc
     3901 tgtttttgat aagatttttg agtctttggt taaaataaac ccatcatctg taggaaaatc
     3961 gacacccgcc atacctttgt gagtcttgtt tgtctttttg ctttttttaa tcggtttaat
     4021 tttgtttgac gtcattccct tacgtgattg tttcgtttta atgggatatg taccattacc
     4081 tcgtgattga caagatatca aagtcaactg agatgataac aatagaccaa ctaaaataat
     4141 aacttttttc gttttcattc aaccctcctt tttatttaac tggttaagta tatcacatca
     4201 cttgtttttc ctcaagctct ttttaggata actattagtt gttgacgagt tggagctctg
     4261 aagcttcaac aaacaatagt ttctgttgtt agtagcatta cacaaaaaaa ctaacttttc
     4321 ttttcctttg aaaataagat cttttataat atactataca tatgaaaaca tcagaaaaaa
     4381 tttatcaatt actgtcacaa accgatgact tcgtcagtgg ggaatatcta gcagatcaac
     4441 tcagtatctc tcgtacatcc gtttggaaat ccatcaaaag tctcgaaaat caggggattc
     4501 aaattgattc tttaaaacac aagggctacc gcatggtaca aggtgatatt ttattaccca
     4561 aaaccattag ccaagggctt gggatgccag tcacctatac ccctcacagc caatctaccc
     4621 aattagatgc caaacaagga atcgaagctc ataacagcgc tccaaggctt tacctagctc
     4681 ctagccaaga agctgccaaa ggacgcctcg atcggcaatt tttttcagct agcaccggtg
     4741 gtatttacat gtccatgtac ctaaaaccca atgtccctta tgctgatatg cctccttata
     4801 cgatgatggt tgcttccagt attgtcaaag ctatctcgcg attaacaggc attgatacag
     4861 aaatcaaatg ggtaaacgac atctatctag gtaaccataa agttgcaggg attctgacag
     4921 aagccattac ttctgttgaa actggtttaa tcaccgatgt cattattggg gttggtctca
     4981 acttttttgt caccgacttc ccagaagcga ttgctcaaaa ggcaggttct ctctttaccg
     5041 aaaaaccgac tatcacgcgc aatgacctta tcattgacat ctggaaatta ttcttatcta
     5101 ttcctgtcaa ggaccatgtc aaagtttaca aagaaaaatc acttgttctt aataaacaag
     5161 tgacctttac agaaaatagt caagaaaaac gtgctattgc gattgatctc acagatcaag
     5221 gacacctcat tgtacagttt gaaaatggtg acttccaaac cttgcgaagc ggtgaaatca
     5281 gtctttcttc ttgggagaat tagccgtttt taaaatggtt ttagtcaatt ggttaatggt
     5341 atgcactctt cttaaatccc taaagagcaa caagccccaa aataaggcga agagataatt
     5401 tccagagagc aacaggtcat taaaaaaata ggtttcaaaa gcacatacaa aggccataaa
     5461 aagaaattgt cgaatagtca tagctttatt ataacagaaa aaaatgattc tatgaagaat
     5521 ccacacagat tgat
//
LOCUS       DATXIG010000076         5504 bp    DNA     linear   BCT 28-DEC-2023
DEFINITION  TPA_asm: Streptococcus pyogenes strain MGAS11113
            SAMN08555799-rid19682583.denovo.076, whole genome shotgun sequence.
ACCESSION   DATXIG010000076 DATXIG010000000
VERSION     DATXIG010000076.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN08555799
            Sequence Read Archive: SRR6740495
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pyogenes
  ORGANISM  Streptococcus pyogenes
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 5504)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5504)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-OCT-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-OCT-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001918058.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001918058.2
            Genome Coverage       :: 368x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/12/2023 11:23:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 1,806
            CDSs (total)                      :: 1,769
            Genes (coding)                    :: 1,722
            CDSs (with protein)               :: 1,722
            Genes (RNA)                       :: 37
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1, 1 (5S, 23S)
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 47
            CDSs (without protein)            :: 47
            Pseudo Genes (ambiguous residues) :: 0 of 47
            Pseudo Genes (frameshifted)       :: 30 of 47
            Pseudo Genes (incomplete)         :: 20 of 47
            Pseudo Genes (internal stop)      :: 9 of 47
            Pseudo Genes (multiple problems)  :: 11 of 47
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5504
                     /organism="Streptococcus pyogenes"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN08555799-rid19682583.denovo.076"
                     /strain="MGAS11113"
                     /serotype="Emm28"
                     /isolation_source="normally sterile site"
                     /host="Homo sapiens"
                     /db_xref="taxon:1314"
                     /country="Finland"
                     /collection_date="2002"
                     /collected_by="National Public Health Laboratory"
     gene            263..412
                     /locus_tag="VC122_001548"
     CDS             263..412
                     /locus_tag="VC122_001548"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014635531.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEP5641464.1"
                     /translation="MRERNEKFIGVLKDMHMQILRDFTNEEDPLTRCELAKGFLEIGD
                     YLSKQ"
     gene            complement(398..613)
                     /locus_tag="VC122_001549"
     CDS             complement(398..613)
                     /locus_tag="VC122_001549"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008087450.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEP5641465.1"
                     /translation="MKISNSKDLALAIVASSSPTLSIEDKIKLYEDSVEAIKKHNLPF
                     VEAEKQEQINNGKVIAEALERGESLFG"
     gene            672..830
                     /locus_tag="VC122_001550"
     CDS             672..830
                     /locus_tag="VC122_001550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017650606.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEP5641466.1"
                     /translation="MNWKKLMFGDLEYTFASRDGKEKTSIEFEGGLLPALLVLGGITW
                     LAAWFITK"
     gene            complement(860..1459)
                     /locus_tag="VC122_001551"
     CDS             complement(860..1459)
                     /locus_tag="VC122_001551"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011284882.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEP5641467.1"
                     /translation="MDLLKNTNFLIPLISAIVSVSSIFISNWLGYRSQIRKLKFDEEK
                     EIYLTLYVPLIKWMNSQSFNNKSYYWLVAFPRYTTNAQDFLTGLLLKNFEKLPVSVAM
                     RYSEYTLNSATSLHFYRNTEYDYDYETFAKKASELFDLIIEQLLTEGTILSQKLSLPN
                     LSKSTLENFLADKKNYIGPRFLSLETHNKPLRPERPLPF"
     gene            1513..1722
                     /locus_tag="VC122_001552"
     CDS             1513..1722
                     /locus_tag="VC122_001552"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017645614.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEP5641468.1"
                     /translation="MRPKKYPYSQKNYPAPKLHNVIETDNQFLIDDKRIHYVIEDSVK
                     TKTLGDGYVEVTLSIIAKSFTKSQG"
     gene            complement(1711..2097)
                     /locus_tag="VC122_001553"
     CDS             complement(1711..2097)
                     /locus_tag="VC122_001553"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000460828.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEP5641469.1"
                     /translation="MFDYFKLYIIVLETFVETKPDDAYSLFDNLSLNSEFVKFSKDLD
                     KNIVISGTLEVIDNLLDDCLIKGKRRPTKDITFYFFDGVTTTGYLYLQSLKDNNFSSR
                     LKNVLKEEGIPLTPTSITRTIAKLTL"
     gene            2171..2398
                     /locus_tag="VC122_001554"
     CDS             2171..2398
                     /locus_tag="VC122_001554"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018166132.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="XRE family transcriptional regulator"
                     /protein_id="HEP5641470.1"
                     /translation="MTKKQRLKQEHLKPKKRLREERLKREYTKMYMADLIGLKNRKGY
                     SEKEDGLQPFKDYEMAIISQKFNVSESELFF"
     gene            complement(2506..3015)
                     /locus_tag="VC122_001555"
     CDS             complement(2506..3015)
                     /locus_tag="VC122_001555"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008088030.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEP5641471.1"
                     /translation="MELFNTIIGVIALIVAVIALVHSIYYNMVKIKLSDCYISRVDKG
                     YDWMYDFSISNLSNVSVIIKKIELYNKDGKLISDNGFNPFQKYEADMQNEADDYYGLS
                     MPNYYMPLDYQWESSPFKSDTEVYPSSRENFSYYLDEKPVKIKITTDKRIHQFRKYQL
                     FFPHFDNNS"
     gene            complement(3274..3483)
                     /locus_tag="VC122_001556"
     CDS             complement(3274..3483)
                     /locus_tag="VC122_001556"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008090780.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEP5641472.1"
                     /translation="MNLEELTPLLNSIDDFETVILHSLVGDFVIDHWIEPNRKNETLI
                     FMHNDQTTELKLSAILGTSTIPKSL"
     gene            complement(3633..3872)
                     /locus_tag="VC122_001557"
     CDS             complement(3633..3872)
                     /locus_tag="VC122_001557"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011284879.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEP5641473.1"
                     /translation="MDTKEFMKVVSKHINQNFNVDNQLVEFVVAELNQMNAPITQKQA
                     QHIVNILEYVSKSTSKSTIAAMTNALLELGVLKGD"
     gene            4039..4224
                     /locus_tag="VC122_001558"
     CDS             4039..4224
                     /locus_tag="VC122_001558"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011054585.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HEP5641474.1"
                     /translation="MWGKIEALLIEKKMTKYELSQKAGLNQNCLIDLKKGRKKSLKFD
                     DVVKIADVLGVSLDEFR"
     gene            4303..4599
                     /locus_tag="VC122_001559"
     CDS             4303..4599
                     /locus_tag="VC122_001559"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011017882.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEP5641475.1"
                     /translation="MAIEIFGPEFRKKLLEDLIALNMEAIKIAQTKNAKSIEWITMKR
                     LEKETGWGRTKLTQWREQGKFNFKRSSENGKVLYDLADVNRFLRTSGYEKGETT"
     gene            4596..4733
                     /locus_tag="VC122_001560"
     CDS             4596..4733
                     /locus_tag="VC122_001560"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011017881.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEP5641476.1"
                     /translation="MKLLDFIFAKPKKQEPIKHDTLRASSEKQWAEFDAYMRKQYGRQ
                     A"
     gene            4814..5143
                     /locus_tag="VC122_001561"
     CDS             4814..5143
                     /locus_tag="VC122_001561"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002984312.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEP5641477.1"
                     /translation="MKTKIEIMRDFFKENPESTQRQASEKLGFTENTVKQYIWRDAKR
                     GYCIKDEEGRVTYLNIEDELSLINEWKSEIRRELIEQLLSANRHETSSEQIRMNAKTI
                     NQILGEI"
     gene            5143..5337
                     /locus_tag="VC122_001562"
     CDS             5143..5337
                     /locus_tag="VC122_001562"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002984315.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEP5641478.1"
                     /translation="MEGMKKYINGQIKLIDTIEECVKEKWPEFDDKKVHKISLAIYQG
                     WSLTDINSTLEAIEMDLDRK"
     gene            5334..>5504
                     /locus_tag="VC122_001563"
     CDS             5334..>5504
                     /locus_tag="VC122_001563"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002984318.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEP5641479.1"
                     /translation="MRIYVNKKGKPSVEFEFEDRRGGMFDTRLMLKESPLKEEFKADM
                     YKAIDDVLKKYED"
BASE COUNT         2010 a          817 c         1043 g         1634 t
ORIGIN      
        1 tctcgttttg agatatacta taatcaagct taagggatta agcaaaaatg aaagtaggta
       61 cagctgatga agtctaggct aaacaaaaag cctaaacaca aagaactaga agtcgaaatc
      121 aagattcttt ggtttaagct taagattcac tactcaatag agtggtgagg ataattagag
      181 ggctaagaag ccctctcccc ctaacggggt aagtttagtt tagcacattg gctgtatctc
      241 cgcaagaatg aaaggagagg ttatgagaga acgaaatgaa aagttcatcg gagtgctaaa
      301 agatatgcat atgcaaattt tgcgagactt cacaaacgaa gaagacccct taacaaggtg
      361 cgagcttgct aaagggttcc tcgaaattgg tgactattta tccaaacaat gactcgccac
      421 gctcaagagc ttcggctata actttaccat tattgatttg ttcctgcttt tcggcttcaa
      481 cgaaaggtaa attatgtttc ttaatagctt ccacagagtc ttcgtaaagt ttgattttat
      541 cttcgataga caaagtaggg ctagaagaag cgacaatagc gagagctaaa tcttttgaat
      601 tagaaatttt cattagctta tcctcctttc cgctaggata agttgattat aacattttta
      661 ggaggcacaa aatgaattgg aaaaaactaa tgtttggcga tttagaatac acgtttgcca
      721 gtcgtgacgg caaagaaaaa acaagcatcg aatttgaagg tggcttattg ccagcgctgt
      781 tggtgttagg tggtatcact tggttagcag cttggtttat tacaaaataa aaagcccaca
      841 aggggcggaa aggggggagt taaaatggca gtggtctttc aggtcttagg ggtttgttat
      901 gggtttctag tgataaaaat ctgggaccga tatagttttt cttgtccgcc aaaaagttct
      961 ctaaagtgga tttggataag tttggtaagc ttagcttttg ggataatatt gtcccttctg
     1021 ttagcaattg ttcaataatg agatcaaata attcagatgc ttttttggca aatgtttcat
     1081 aatcataatc gtattcggtg ttacgataaa aatgtaaaga ggttgcagaa tttagggtgt
     1141 attcagagta tctcatagca actgatactg gtaatttttc aaaatttttt aacaacaagc
     1201 ctgtcagaaa atcttgtgca tttgttgtat atctagggaa cgccactagc caataataac
     1261 ttttgttatt gaatgattgc gaattcatcc attttattaa cggtacatag agggttaggt
     1321 atatttcttt ttcttcatca aattttaatt tcctgatttg acttctataa ccaagccaat
     1381 tgcttataaa aatagaagaa acagagacta ttgctgaaat taacggaatt aaaaaattgg
     1441 tatttttcaa taaatccatg attttctcca atcattttat ttaaattata ccacagaaag
     1501 ggggtggaat agatgcgccc caaaaaatat ccgtatagcc aaaaaaatta tccagcgccg
     1561 aagcttcata acgtcattga aaccgataat cagttcttaa tagatgataa aaggattcat
     1621 tatgttattg aagattctgt aaagacaaaa acccttggcg atggctatgt tgaagtaaca
     1681 ctttccataa ttgccaagag ttttacaaaa tcacaaggtt agtttggcga tagtccttgt
     1741 gatagaagtt ggtgtaagag ggataccttc ttctttaagg acattcttta aacggctcga
     1801 aaaattatta tctttaagac tttgaagata taagtaacca gttgttgtta cgccatcgaa
     1861 aaaatagaaa gtaatatctt ttgttgggcg acgttttcct tttatcaagc aatcatcaag
     1921 taaattatca attacttcta aagtgcctga tattacaatg tttttatcta agtctttaga
     1981 gaattttaca aattcactgt taagacttag attatcaaat aagctataag cgtcgtctgg
     2041 tttggtttcc acgaaggttt ccaagacaat tatataaagt ttgaaatagt caaacatcac
     2101 attcacctcc tttctgccca tattatagca gattggagga actaaaaaca gatagaaagg
     2161 aggtggggga atgacgaaaa aacaacgttt aaaacaggaa catctcaaac ctaaaaagcg
     2221 tttaagagaa gaacgtttaa aacgtgagta cacaaaaatg tatatggctg atttaattgg
     2281 tttaaaaaat agaaaaggtt actctgagaa agaagatggt ttacagcctt ttaaagatta
     2341 tgaaatggct atcatttctc aaaaatttaa tgtttcagag agtgaattat ttttttaaac
     2401 aaaaatatct caaaacgaga taaagtgtac aacactagaa aggataaaag aaaaatgctc
     2461 ctaacggcag cacttatctt aattttatta acgaatatcg ctatcttaac tattattgtc
     2521 aaaatgggga aagaataact gatattttcg gaattgatga atacgcttat cagttgtgat
     2581 tttgatttta actggctttt catctaaata gtaagagaaa ttttctcgac tagatggata
     2641 tacttcagta tctgacttaa atggcgatga ctcccattgg taatctaatg gcatataata
     2701 attcggcatt gacaatccat aataatcatc agcttcattc tgcatgtcag cttcgtattt
     2761 ttgaaaggga ttgaagccat tatcgcttat tagttttcca tctttgttgt aaagttcaat
     2821 ttttttaata atgactgaaa cattcgataa gttactgata ctaaaatcat acatccaatc
     2881 gtaaccttta tctactcttg aaatgtaaca atcagataat tttatcttaa ccatgttgta
     2941 gtagatagag tgaacaagag caatcacagc aacaattagc gcaatgacgc cgataattgt
     3001 attaaataat tccattttta ttcttccaat cgtttttatt ttaattatac caaaatagaa
     3061 aggacacata tgaacgaaat agaaaacaaa ctagaagaac ttgaagaaat ggtaataaac
     3121 atggatgaag tagatgtagt gattccatgg aagatagcaa aaaacctgct acaaagagca
     3181 ggttacttat cagaagagga acatcgccta ctaagttgga gactcggaaa atcaaaatat
     3241 gcaggcaaac gttccgaaaa agtccgcaat ttgctagagg gacttaggaa tagtggaagt
     3301 tccgagaata gctgataatt ttaattctgt tgtttggtca ttgtgcatga aaatgagagt
     3361 ttcatttttg cgattaggct caatccaatg atcaataaca aagtctccga caagactatg
     3421 taaaataaca gtttcaaaat catcaatact attcaataga ggagttaatt cttctaaatt
     3481 catttagtta ccctccttcc taataggata actacattat acaacagaaa ggaagcacat
     3541 gagaccaaaa cgatatccgt atagcggaga gaaaaagaga gcctatcata agatagaccc
     3601 tgaactgata aaaaccggta aagttgattg catcaatctc ccttaagtac gccaagctct
     3661 aacaatgcat ttgtcatagc tgcgatagta gatttagagg ttgacttaga gacatactcc
     3721 aaaatattaa cgatatgctg agcttgcttt tgtgtaatag gtgcattcat ttgattaagt
     3781 tccgcaacga caaattcaac taattggttg tcaacattga aattttggtt gatatgtttt
     3841 gagacaactt tcataaattc ttttgtatcc ataattaatc tcctttcttg cttatttgac
     3901 taaacaagat tagaagagta gaggtctctc gagagatctt gttgaatcat acgaatatta
     3961 tatcaagact atattgaaaa atcaatatat tgtatataaa aagttatcaa caactacatg
     4021 ttgtgcttag gaggaattat gtggggtaaa atcgaagctc tattaattga gaaaaaaatg
     4081 accaaatatg aactgtcaca aaaggctgga ttgaaccaaa attgcttgat tgatctaaag
     4141 aaaggaagaa aaaaatcatt gaaatttgat gatgtcgtta aaattgccga tgtcttaggt
     4201 gtcagcttag acgaatttag ataacaaaaa aagtccgacg gcaatcggac tcaaaacaaa
     4261 ttttaattta cttaattata acataagaga gagggaaaaa ctatggccat tgaaatattt
     4321 ggacctgagt ttagaaaaaa actgcttgaa gatttaatcg ctctaaatat ggaagcgata
     4381 aaaatagcgc agaccaaaaa cgccaagtct attgaatgga taaccatgaa gcggctagaa
     4441 aaagaaactg gatgggggcg aactaaattg acccagtgga gagaacaagg gaaatttaac
     4501 tttaaaaggt catcagaaaa cgggaaagta ctatatgacc tagcagatgt taatagattt
     4561 ttacggacca gtggatatga aaaaggagaa acaacatgaa attattagat tttatttttg
     4621 caaaaccaaa aaaacaggaa ccgattaagc acgacacgct tagagcatca tctgaaaagc
     4681 agtgggccga atttgatgcc tatatgagga aacagtatgg acgacaagct taatcagata
     4741 ttgatagcca tgcgaaacta ccgcacaaat ggtgatgata gcaagtattt agaggatatg
     4801 gaggtcattt taaatgaaaa caaaaattga aatcatgcgt gattttttta aagaaaatcc
     4861 tgaatcaact caacggcaag cctcagaaaa gcttggattt acggaaaata cagttaaaca
     4921 atatatttgg agagatgcta agcgtggcta ctgtattaaa gacgaagaag gtcgtgtaac
     4981 ttatctaaac attgaagatg aattatcgct tattaatgaa tggaaaagtg aaattagaag
     5041 agaattgatt gaacagttac tatcagctaa tcgacatgaa acgtcaagcg aacaaattcg
     5101 tatgaacgct aaaacaatca atcagatttt aggagaaatt taatggaagg tatgaaaaag
     5161 tatatcaatg gtcagattaa actaattgat actattgaag aatgtgtcaa agaaaaatgg
     5221 cctgaatttg atgataaaaa agtacataag atatctttag ctatttacca aggatggtcc
     5281 cttacagata ttaatagcac actcgaagcg atagaaatgg acttggatag aaaatgagaa
     5341 tttatgtaaa caaaaaaggt aaaccatctg ttgaatttga gtttgaagat cgacgcggtg
     5401 gcatgtttga tacaagatta atgttaaaag aatcaccact caaagaagaa tttaaagccg
     5461 atatgtacaa ggcaattgat gatgttttaa aaaaatacga agac
//
LOCUS       DATXIG010000077         5100 bp    DNA     linear   BCT 28-DEC-2023
DEFINITION  TPA_asm: Streptococcus pyogenes strain MGAS11113
            SAMN08555799-rid19682583.denovo.077, whole genome shotgun sequence.
ACCESSION   DATXIG010000077 DATXIG010000000
VERSION     DATXIG010000077.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN08555799
            Sequence Read Archive: SRR6740495
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pyogenes
  ORGANISM  Streptococcus pyogenes
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 5100)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5100)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-OCT-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-OCT-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001918058.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001918058.2
            Genome Coverage       :: 368x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/12/2023 11:23:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 1,806
            CDSs (total)                      :: 1,769
            Genes (coding)                    :: 1,722
            CDSs (with protein)               :: 1,722
            Genes (RNA)                       :: 37
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1, 1 (5S, 23S)
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 47
            CDSs (without protein)            :: 47
            Pseudo Genes (ambiguous residues) :: 0 of 47
            Pseudo Genes (frameshifted)       :: 30 of 47
            Pseudo Genes (incomplete)         :: 20 of 47
            Pseudo Genes (internal stop)      :: 9 of 47
            Pseudo Genes (multiple problems)  :: 11 of 47
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5100
                     /organism="Streptococcus pyogenes"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN08555799-rid19682583.denovo.077"
                     /strain="MGAS11113"
                     /serotype="Emm28"
                     /isolation_source="normally sterile site"
                     /host="Homo sapiens"
                     /db_xref="taxon:1314"
                     /country="Finland"
                     /collection_date="2002"
                     /collected_by="National Public Health Laboratory"
     gene            579..3605
                     /locus_tag="VC122_001564"
     CDS             579..3605
                     /locus_tag="VC122_001564"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011184393.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="leucine-rich repeat protein"
                     /protein_id="HEP5641480.1"
                     /translation="MKKHLKTVALTLTTVSVVTHNQEVFSLVKEPILKQTQASSSISG
                     ADYAESSGKSKLKINETSGPVDDTVTDLFSDKRTTPEKIKDNLAKGPREQELKAVTEN
                     TESEKQITSGSQLEQSKESLSLNKTVPSTSNWEICDFITKGNTLVGLSKSGVEKLSQT
                     DHLVLPSQAADGTQLIQVASFAFTPDKKTAIAEYTSRAGENGEISQLDVDGKEIINEG
                     EVFNSYLLKKVTIPTGYKHIGQDAFVDNKNIAEVNLPESLETISDYAFAHLALKQIDL
                     PDNLKAIGELAFFDNQITGKLSLPRQLMRLAERAFKSNHIKTIEFRGNSLKVIGEASF
                     QDNDLSQLMLPDGLEKIESEAFTGNPGDDHYNNRVVLWTKSGKNPYGLATENTYVNPD
                     KSLWQESPEIDYTKWLEEDFTYQKNSVTGFSNKGLQKVKRNKNLEIPKQHNGVTITEI
                     GDNAFRNVDFQNKTLRKYDLEEVKLPSTIRKIGAFAFQSNNLKSFEASDDLEEIKEGA
                     FMNNRIETLELKDKLVTIGDAAFHINHIYAIVLPESVQEIGRSAFRQNGANNLIFMGS
                     KVKTIGEMAFLSNRLEHLDLSEQKQLTEIPVQAFSDNALKEVLLPASLKTIREEAFKK
                     NHLKQLEVASALSHIAFNALDDNDGDEQFDNKVVVKTHHNSYALADGEHFIVDPDKLS
                     STMVDLEKILKLIEGLDYSTLRQTTQTQFRDMTTAGKALLSKSNLRQGEKQKFLQEAQ
                     FFLGRVDLDKAIAKAEKALVTKKATKNGQLLERSINRAVLAYNNSAIKKANVKRLEKE
                     LDLLTGLVEGKGPLAQATMVQGVYLLKTPLPLPEYYIGLNVYFDKSGKLIYALDMSDT
                     IGEGQKDAYGNPILNVDEDNEGYHALAVATLADYEGLDIKTILNSKLSQLTSIRQVPT
                     AAYHRAGIFQAIQNAAAEAEQLLPKPGTHSEKSSSSESANSKDRGLQSNPKTNRGRHS
                     AILPRTGSKGSFVYGILGYTSVALLSLITAIKKKKY"
     gene            3625..4026
                     /locus_tag="VC122_001565"
     CDS             3625..4026
                     /locus_tag="VC122_001565"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002985067.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxamine 5'-phosphate oxidase family
                     protein"
                     /protein_id="HEP5641481.1"
                     /translation="MITQEMKDLINNQLAMVATVDAKGQPNIGPKRSMRLWDDKTFIY
                     NENTDGQTRINIEDNGKIEIAFVDRERLLGYRFVGTAEIQTEGAYYEAAKKWAQGRMG
                     VPKAVGIIHVERIFNLQSGANAGKEINSESN"
     gene            complement(4216..5091)
                     /locus_tag="VC122_001566"
     CDS             complement(4216..5091)
                     /locus_tag="VC122_001566"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011284685.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cation transporter"
                     /protein_id="HEP5641482.1"
                     /translation="MPASKKVTIIFILNLSFSLIEFIFGTLFFSGAILADAVHDFGDA
                     IAIGISAILERKAVKKESPNFSLGYKRFSLLGALTTNLILISGSLLVMIETIPKLWHP
                     TIVNYDGMFVLAIFAIIINGFASFIIHSNQTKNEEILSLHFLEDILGWLAIIILSLIL
                     KWKPWYILDPLLSIAIASFILSKALPKLVATANIFLDGVPDSIDYCALHHELSQLPHI
                     VSVNQLNVWSMDGIDHRATIHCCLKESTTEKHCKKSIRLICQRYNINSVTVEIDTSLN
                     EHQHHCSSLSGIEVN"
BASE COUNT         1808 a          792 c          968 g         1532 t
ORIGIN      
        1 tatattcttt acattctgat gaaaatacaa attatcctaa catgatgtat tgaaaggaga
       61 aatagtttat gttagtatac tgattaagtg aagctgtaat gaggcttata cgatcaaaaa
      121 agttagagta actttataaa acagtggtgt tgaagcaagt ttagcttctt ctcagaaata
      181 gattatctat aaaaaatccc aaaagtagtt tgtttccagt taatgaactt cttttgggaa
      241 ggcttttttg ctgatcgcca cttcacttgt atatcgggaa cattcacgct aagatgtata
      301 agttgaatcg tttgtctatg gcttgatgaa aattaatcaa aagataatgt tcaactctat
      361 aattagtaca agcaaaatga gataacttat tcattagtaa gcataagata taacaagaga
      421 aattacctta aaacaggaag tactagagta tttaaaataa gtgatagact tggatattat
      481 taggtcgact agtgttcttt tcaataattt aatatcgtta ttattagtat cagccaatca
      541 aatgtggata agttggcttt tagaaaggtt ttaccacaat gaagaaacat cttaaaacag
      601 ttgccttgac cctcactaca gtatcggtag tcacccacaa tcaggaagtt tttagtttag
      661 tcaaagagcc gattcttaaa caaactcaag cttcttcatc gatttctggc gctgactacg
      721 cagaaagtag cggtaaaagc aagttaaaga ttaatgaaac ttctggccct gttgatgata
      781 cagtcactga cttattttcg gataaacgta ctactcctga aaaaataaaa gataatcttg
      841 ctaaaggtcc gagagaacaa gagttaaagg cagtaacaga gaatacagaa tcagaaaagc
      901 agatcacttc tggatctcaa ctagaacaat caaaagagtc tctttcttta aataaaacag
      961 tgccatcaac gtctaattgg gagatttgtg attttattac taaggggaat acccttgttg
     1021 gtctttcaaa atcaggtgtt gaaaagttat ctcaaactga tcatctcgta ttgcctagtc
     1081 aagcagcaga tggaactcaa ttgatacaag tagctagttt tgcttttact ccagataaaa
     1141 agacggcaat tgcagaatat accagtaggg ctggagaaaa tggggaaata agccaactag
     1201 atgtggatgg aaaagaaatt attaacgaag gtgaggtttt taattcttat ctactaaaga
     1261 aggtaacaat cccaactggt tataaacata ttggtcaaga tgcttttgtg gacaataaga
     1321 atattgctga ggttaatctt cctgaaagcc tcgagactat ttctgactat gcttttgctc
     1381 acctagcttt gaaacagatc gatttgccag ataatttaaa agcgattgga gaattagctt
     1441 tttttgataa tcaaattaca ggtaaacttt ctttgccacg tcagttaatg cgattagcag
     1501 aacgtgcttt taaatcaaac catatcaaaa caattgagtt tagaggaaat agtctaaaag
     1561 tgatagggga agctagtttt caagataatg atctgagtca actaatgcta cctgacggtc
     1621 ttgaaaaaat agaatcagaa gcttttacag gaaatccagg agatgatcac tacaataacc
     1681 gtgttgtttt gtggacaaaa tctggaaaaa atccttatgg tcttgctact gaaaatacct
     1741 atgttaatcc tgataagtca ctatggcagg aaagtcctga gattgattat actaaatggt
     1801 tagaggaaga ttttacctat caaaaaaata gtgttacagg tttttcaaat aaaggcttac
     1861 aaaaagtaaa acgtaataaa aacttagaaa ttccaaaaca gcacaatggt gttactatta
     1921 ctgaaattgg tgataatgct tttcgcaatg ttgattttca aaataaaact ttacgtaaat
     1981 atgatttgga agaagtaaag cttccctcaa ctattcggaa aataggtgct tttgcttttc
     2041 aatctaataa cttgaaatct tttgaagcaa gtgacgattt agaagagatt aaagagggag
     2101 cctttatgaa taatcgtatt gaaaccttgg aattaaaaga taaattagtt actattggtg
     2161 atgcggcttt ccatattaat catatttatg ccattgttct tccagaatct gtacaagaaa
     2221 tagggcgttc agcatttcgg caaaatggtg caaataatct tatttttatg ggaagtaagg
     2281 ttaagaccat aggtgagatg gcatttttat caaatagact tgaacatctg gatctttctg
     2341 agcaaaaaca gttaacagag attcctgttc aagccttttc agacaatgcc ttgaaagaag
     2401 tattattacc agcatcactg aaaacgattc gagaagaagc cttcaaaaag aatcatttaa
     2461 aacaactgga agtggcatct gccttgtccc atattgcttt taatgcttta gatgataatg
     2521 atggtgatga acaatttgac aataaagtgg ttgttaaaac gcatcataat tcctacgcac
     2581 tagcagatgg tgagcatttt atcgttgatc cagataagtt atcttctaca atggtagacc
     2641 ttgaaaagat tttaaaacta atcgaaggtt tagattattc tacattacgt cagactactc
     2701 aaactcagtt tagagacatg actactgcag gtaaagcgtt gttgtcaaaa tctaacctcc
     2761 gacaaggaga aaaacaaaaa ttccttcaag aagcacaatt tttccttggc cgcgttgatt
     2821 tggataaagc catagctaaa gctgagaagg ctttagtgac caagaaggca acaaagaatg
     2881 gtcagttgct tgaaagaagt attaacagag cggtattagc ttataataat agcgctatta
     2941 aaaaagctaa tgttaagcgc ttggaaaaag agttagactt gctaacagga ttagttgagg
     3001 gaaaaggacc attagcgcaa gctacaatgg tacaaggagt ttatttatta aagacgcctt
     3061 tgccattgcc agagtattat atcggattga acgtttattt tgacaagtct ggaaaattga
     3121 tttatgcact tgatatgagt gatactattg gcgagggaca aaaagacgct tatggtaatc
     3181 ctatattaaa tgttgacgag gataatgaag gttatcatgc cttggcagtt gccactttag
     3241 ctgattatga ggggctcgac atcaaaacaa ttttaaatag taagcttagt caattaacat
     3301 ctattcgtca ggtaccgact gcagcctatc atagagccgg tattttccaa gctatccaaa
     3361 atgcagcggc agaagcagag cagttattgc ctaaaccagg tacgcactct gagaaatcaa
     3421 gctcaagtga atctgctaac tctaaagata gaggattgca atcaaaccca aaaacgaata
     3481 gaggacgaca ctctgcaata ttgcctagga cagggtcaaa aggcagcttt gtctatggaa
     3541 tcttaggtta cactagcgtt gctttactgt cactaataac tgctataaaa aagaaaaaat
     3601 attaaattga tttaaggaga agaaatgata acacaagaaa tgaaagatct tattaataac
     3661 caattagcaa tggttgcgac ggttgatgcc aaaggtcaac ccaatattgg tccaaaacgt
     3721 tctatgcgtc tctgggatga taaaacattt atttataatg aaaatacaga tggtcaaact
     3781 cgtattaaca ttgaagataa tgggaaaatt gaaattgcct ttgttgatcg tgagcgcctt
     3841 ttaggttaca ggtttgtagg aactgctgaa attcaaacag aaggtgctta ttacgaagca
     3901 gcgaaaaaat gggcacaagg acgcatgggg gtaccaaaag cagttggtat tattcatgtc
     3961 gagcgtattt ttaatttaca atctggtgct aatgcaggta aagaaataaa cagtgaaagt
     4021 aactaaaatt aactaaaatt aactgtaaca gtatttagga cccttatttt aaatgctaaa
     4081 aaacaagcat actctttttt acagcatagt gactatgttg taaaaagggg tatgcttatt
     4141 aggtgctctt aatatttcta gataactgca cctaataagt tttcaataga cttatagcac
     4201 attagttgac ttcaattagt tgacttcaat accagaaaga gaagaacagt gatgttggtg
     4261 ttcgtttaaa gaagtatcga tttccacagt gactgagttt atattgtacc tttgacaaat
     4321 cagtctaata gattttttgc aatgtttttc agtggtagat tctttcaagc agcaatgtat
     4381 agttgctcta tggtcaatac catccatcga ccaaacatta agctgattaa cagacactat
     4441 atgaggaagt tggctgagtt catggtgcaa agcgcaatag tctatcgaat caggaacacc
     4501 atctaaaaaa atgttagcgg ttgctactaa ctttggaaga gctttagata atataaaaga
     4561 agcaatggca attgataata aaggatcaag aatgtaccaa ggtttccatt ttaagatcag
     4621 tgacagtatg ataatggcta accatccaag gatatcttct aaaaagtgaa ggcttaatat
     4681 ttcttcattt tttgtctggt tagagtgaat gatgaagcta gcaaatccat tgattataat
     4741 tgcaaaaata gctaaaacga acataccgtc ataattaaca atagtaggat gccataattt
     4801 tggtatcgtt tcaatcatta ctaataatga cccgctaata agtattagat ttgtcgttag
     4861 cgctcctaaa aggctaaatc gcttatagcc tagtgaaaaa tttggactct cttttttaac
     4921 agcctttctt tctaagatag ctgagatacc aatagcaatg gcatctccaa aatcgtggac
     4981 agcatctgct aaaatagcac ccgaaaagaa taatgttcca aaaataaatt caatgaggga
     5041 aaaactaaga tttaatataa aaataatggt gactttttta cttgctggca taattttctc
//
LOCUS       DATXIG010000078         5034 bp    DNA     linear   BCT 28-DEC-2023
DEFINITION  TPA_asm: Streptococcus pyogenes strain MGAS11113
            SAMN08555799-rid19682583.denovo.078, whole genome shotgun sequence.
ACCESSION   DATXIG010000078 DATXIG010000000
VERSION     DATXIG010000078.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN08555799
            Sequence Read Archive: SRR6740495
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pyogenes
  ORGANISM  Streptococcus pyogenes
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 5034)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5034)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-OCT-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-OCT-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001918058.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001918058.2
            Genome Coverage       :: 368x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/12/2023 11:23:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 1,806
            CDSs (total)                      :: 1,769
            Genes (coding)                    :: 1,722
            CDSs (with protein)               :: 1,722
            Genes (RNA)                       :: 37
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1, 1 (5S, 23S)
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 47
            CDSs (without protein)            :: 47
            Pseudo Genes (ambiguous residues) :: 0 of 47
            Pseudo Genes (frameshifted)       :: 30 of 47
            Pseudo Genes (incomplete)         :: 20 of 47
            Pseudo Genes (internal stop)      :: 9 of 47
            Pseudo Genes (multiple problems)  :: 11 of 47
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5034
                     /organism="Streptococcus pyogenes"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN08555799-rid19682583.denovo.078"
                     /strain="MGAS11113"
                     /serotype="Emm28"
                     /isolation_source="normally sterile site"
                     /host="Homo sapiens"
                     /db_xref="taxon:1314"
                     /country="Finland"
                     /collection_date="2002"
                     /collected_by="National Public Health Laboratory"
     gene            193..1500
                     /gene="eno"
                     /locus_tag="VC122_001567"
     CDS             193..1500
                     /gene="eno"
                     /locus_tag="VC122_001567"
                     /EC_number="4.2.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002985288.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphopyruvate hydratase"
                     /protein_id="HEP5641483.1"
                     /translation="MSIITDVYAREVLDSRGNPTLEVEVYTESGAFGRGMVPSGASTG
                     EHEAVELRDGDKSRYLGLGTQKAVDNVNNIIAEAIIGYDVRDQQAIDRAMIALDGTPN
                     KGKLGANAILGVSIAVARAAADYLEVPLYTYLGGFNTKVLPTPMMNIINGGSHSDAPI
                     AFQEFMIMPVGAPTFKEGLRWGAEVFHALKKILKERGLVTAVGDEGGFAPKFEGTEDG
                     VETILKAIEAAGYEAGENGIMIGFDCASSEFYDKERKVYDYTKFEGEGAAVRTSAEQV
                     DYLEELVNKYPIITIEDGMDENDWDGWKVLTERLGKRVQLVGDDFFVTNTEYLARGIK
                     ENAANSILIKVNQIGTLTETFEAIEMAKEAGYTAVVSHRSGETEDSTIADIAVATNAG
                     QIKTGSLSRTDRIAKYNQLLRIEDQLGEVAQYKGIKSFYNLKK"
     gene            complement(1982..3010)
                     /locus_tag="VC122_001568"
                     /pseudo
     CDS             complement(1982..3010)
                     /locus_tag="VC122_001568"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014677767.1"
                     /note="frameshifted; internal stop; incomplete; partial in
                     the middle of a contig; missing N-terminus; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
     gene            complement(3290..4831)
                     /locus_tag="VC122_001569"
     CDS             complement(3290..4831)
                     /locus_tag="VC122_001569"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011284650.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HEP5641484.1"
                     /translation="MLHLHLETKLQDKLSLLNILLDVSEVSIDQLCQETELKKQRVYN
                     LLFEMIKDLEDTLTLTICDDTVSIPYKTYQLKMPYFKKLYQTSIFLKMLCFLIEPGEL
                     SLHDFIKREYISQATAYRIRTNCRKYLKKVGLNVRQNHIVGPEYRIRFLIALLHYQFG
                     MTIYDFDKTSMNKVVSLIINSNQAITLNDASKAPYEFSYFAILISLIWKRRHDNLGIP
                     QTDAFKHLKKLSIYRDIKMTSQEIIGKWYHPDLTDEDLDYIFLCFCTTNNSFHKDKWT
                     PKKVKELFELVMTKTNGKTLKASLRPLLGNNILNSLPFKRILVSFSRLFISNLQVLLP
                     DIHLFHYLRRQQKRNKSFYNTLKTIVEEWMSAEGIVGKLPSYHLLLFTIQLEELLKTY
                     LPPIPVYLLTNNTAALDLMTNALSIYFPPAIATVMPVNVEIIPFKDIVKEKQSVIIAD
                     RQYLNLIQHLYQNQGHLFLYFLFSFRDVSEAYIHKAFLDFRQKRYDEFIVTLLDTYHK
                     NLSSP"
BASE COUNT         1554 a          886 c         1050 g         1544 t
ORIGIN      
        1 tatatcgatt tctacaataa aataccaatt tgaaaatttt cggtaaatgt gttaaaaaac
       61 aagaaagaaa gtgatttttc ttgcaaaaaa caaaggaagc ttttcatgag attgcttttt
      121 aagttgatta aaggcctagt ttgtggtatt atgaacatgt aaaatattaa aactcataag
      181 gagagtatta caatgtcaat tattactgat gtatacgctc gcgaagtcct tgactcacgc
      241 ggtaacccaa cacttgaagt agaagtttat acagaatcag gtgcattcgg acgtggtatg
      301 gttccttcag gagcttcaac tggtgaacat gaagccgttg aacttcgtga tggtgacaaa
      361 tctcgttacc ttggtctagg tactcaaaaa gcggttgata acgttaataa cattatcgct
      421 gaggcaatca tcggttacga tgttcgcgat caacaagcta tcgaccgtgc aatgatcgct
      481 cttgacggta ctcctaacaa aggtaaactt ggtgctaatg ctattcttgg tgtttctatc
      541 gctgttgctc gtgcagctgc tgactatctt gaagtgccac tttacactta ccttggcgga
      601 ttcaacacta aagttcttcc aactcctatg atgaacatca tcaacggtgg atcacactca
      661 gatgccccaa ttgcattcca agagttcatg atcatgccag ttggtgcacc tactttcaaa
      721 gaaggtcttc gttggggtgc tgaagttttc cacgctctta agaaaattct taaagaacgt
      781 ggacttgtta cagctgtagg tgacgaaggt ggatttgctc ctaaatttga aggaactgaa
      841 gacggtgtag aaactatcct taaagctatt gaagcagctg gttacgaagc tggtgaaaac
      901 ggcattatga tcggttttga ctgtgcatca tcagaattct acgacaaaga acgtaaagtt
      961 tacgactaca ctaaatttga aggtgaaggc gctgctgttc gtacatctgc agaacaagtt
     1021 gattaccttg aagagttggt taacaaatac ccaattatta ctatcgaaga tggtatggat
     1081 gaaaacgact gggatggttg gaaagttctt actgaacgcc taggcaaacg tgttcaattg
     1141 gttggtgacg acttcttcgt tacaaacact gaataccttg ctcgtggtat caaagaaaat
     1201 gcagctaact caatccttat caaagttaac caaatcggta ctttgactga aacgtttgaa
     1261 gctatcgaaa tggctaaaga agctggatat actgccgttg tatcacaccg ttcaggtgaa
     1321 actgaagatt caacaatcgc tgacatcgca gttgcaacaa acgctggcca aatcaaaaca
     1381 ggttcattgt cacgtacaga ccgtattgct aaatacaacc aattacttcg tatcgaagat
     1441 caacttggtg aagttgctca atacaaaggt atcaaatcat tctataactt aaaaaaatag
     1501 acggtaagac agaaatcggt cattcgtaga atgcgatttc gtcgtcgcac ccccgtcagg
     1561 ctgactaggt tggtcacgac aaaaggagtg aacatccagt cagctacgac gcaaaagtta
     1621 aagattaaag aatagaaagc ttgggaaaat cccaagcttt ttgctttcta aatagtaaaa
     1681 atttactgtg ggctaattta tcctgagcta atgtattagc gaggtaaagt tattgaggaa
     1741 ggcttggatt tgtactgcct ctaaaaagtt ggacactata ttttaaatac aggactaagt
     1801 ccttttatac caattgtcaa gcacatcaag gttttaactg aaaaaatgtt ttagacaata
     1861 gcctaggttt agctgttgtc ttacttgttg tgctctgatt agcataaaaa agtgtacaag
     1921 aaaaaaacct tgcctagtat ttttaagatc aaggtgttac aatgatagag caacaacgac
     1981 tttaatgatt ttgggtcgaa gcgtaattgt aaagtttgtt atgcgttatg aggtaaagca
     2041 ttgtctgaca aggcaatgtc tagaggcaat cgtatgtggc ttggttgaag ctgtttgcga
     2101 ttgccttttt cgtttctcat agaagtctgc gatatgacaa ggattggtat gactagcaaa
     2161 agcaatgtca tggatacact tgaaaagaat ctttgaagca taaggattcc cacgtttggt
     2221 aatgtgctct tgagctagat agttgccaga ctcataatgt ctaaagtcaa tcccaatgaa
     2281 agcattaatt tgattagcag attgaaaacg acgaatagca ccgagttcgc caataatact
     2341 tgttgcagtt gtttctgcga tacttggaat agagcgtaga atcttgtctt ctggtaaagg
     2401 ttgagccaac gccaccatct tatcaaaaat agcttgacga cgttcagaaa gtctaagtaa
     2461 ttctttggca tagtagcgaa cctcttctat catcggagag gttttcgtaa cagtaaaata
     2521 agactgcttg gctaatgaga caagtttttc cactaagtag atcccacgct tgtcagagat
     2581 acgtttagag gtagattgac ggatgatagc tgacaatgct ttgtcagcta gctccagtac
     2641 aaagctatta catggaaaag ccgttaccac attccagtat tactcaccag ttggtgcaaa
     2701 taagatgtgt tccagttcag gaaaagtgac ttgtaagatc ttgtgaaggc gattcttaac
     2761 tctcgaacaa tattatcctc agtcaatttt tgatagaagc gactcaagtc acgtagcagc
     2821 tgataaactt cttcctgaac gtaagttggt tttctaacta gaataaattg agactgagcc
     2881 aatttttcag tgtctaactt atccgcttta cgcacccgta gactatctag ttttctaaag
     2941 gattgagttg tgtataagcg tagccatttt tctcaaggaa ggactgaagc cgacgagaat
     3001 agactcctgt tgtgatggtc tcccttttgc cttttaaaca atagtatagc cgtttttcaa
     3061 gatgttatta atgatttttt cctttattct agaagaataa gtctgatact gtttcttctt
     3121 aataatctca atgccctaac agtccatcag tcgaacggta tttctcaaat tactgcagtt
     3181 gactcttgcg ttcagttccc tcaccttgtg gatgccgttt agaacaatat tagcttgctg
     3241 aattagcttg ctgagccttt tggtaatgtg aacaaagcat ctttatcact caaggagaag
     3301 acaggttctt gtggtaagtg tccaacaagg tcacgataaa ctcatcgtac cgtttttggc
     3361 ggaagtctag aaatgcctta tgaatgtaag cctcagaaac gtctctaaaa ctaaacaaga
     3421 aatacaagaa caaatgccct tgattctggt acaaatgctg tatcaggttc aggtattgcc
     3481 tatctgcgat aatgacactc tgcttttcct taacaatatc tttgaaagga atgatttcga
     3541 cattgacagg cataaccgtt gcaatagctg gcgggaagta aatcgagagt gcattggtca
     3601 tcaaatccag cgcagcagtg ttattggtca aaagatagac aggtatcggt ggcaaatatg
     3661 tttttaggag ttcctctaac tgtatcgtga agagcagtaa atgataggac ggcagtttcc
     3721 ccacgatgcc ttctgcgctc atccattcct caacgatggt ttttagagta ttgtagaagc
     3781 tcttgtttcg tttttgctgt cttctgaggt aatggaacaa gtgtatatca ggtaacaaaa
     3841 cttgaagatt actgataaat agacgtgaga aggaaaccaa aatccgtttg aaaggaaggg
     3901 agtttaggat attattgcct agtagtggac gcagggatgc tttcaaggtt tttccatttg
     3961 tttttgtcat caccaactca aacaattctt tcactttttt aggtgtccat ttatccttat
     4021 ggaaagaatt attggtggtg caaaaacata agaaaatata atccaaatcc tcatcagtta
     4081 aatcaggatg gtaccatttt ccgatgattt cctgactggt cattttgata tctcgataga
     4141 tagacagttt tttcaagtgt ttgaatgcat ctgtctgagg gatccccaag ttatcatgtc
     4201 gccgtttcca tataagggat atcagaatgg caaagtatga aaattcataa ggggctttac
     4261 tggcatcatt aagtgtgatg gcttgattgg agtttataat cagcgaaacc actttattca
     4321 ttgatgtttt atcaaaatca taaatggtca taccaaactg atagtgcaac aaggcgatca
     4381 gaaagcggat acggtattct ggtccaacaa tatggttttg ccgaacgttt aaacctacct
     4441 ttttcaagta tttccgacaa ttggtcctga tacggtaagc ggtggcttgg gaaatatact
     4501 ctcgtttaat gaagtcgtgt aggctcagct ctcctggttc aattaaaaaa cataacatct
     4561 ttaagaatat cgaggtttga tagagtttct taaaataagg cattttaagt tggtaggtct
     4621 tataggggat agaaacagtg tcgtcacaaa tggttaaggt gagggtgtct tctaaatcct
     4681 taatcatttc aaagagtaag ttgtagacac gttgtttttt gagttcggtt tcctgacaca
     4741 actggtctat ggagacttcg gaaacgtcta acaaaatgtt gagcagtgat aatttatctt
     4801 gtaatttggt ttctagatga aggtgtaaca taactgaact cctttcattt ttatagcaga
     4861 aactcgtatt tatttaatta tcacataatt aaataaaaca gtcaaataat tcaatggtga
     4921 cgcgtgttta aggagtattg agaatgtcag atataagcaa aatgcagctg aatattctta
     4981 attttaggta tgaaaaagcg tcgggattga taaaaaatct attgaattgg gtaa
//
LOCUS       DATXIG010000079         5012 bp    DNA     linear   BCT 28-DEC-2023
DEFINITION  TPA_asm: Streptococcus pyogenes strain MGAS11113
            SAMN08555799-rid19682583.denovo.079, whole genome shotgun sequence.
ACCESSION   DATXIG010000079 DATXIG010000000
VERSION     DATXIG010000079.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN08555799
            Sequence Read Archive: SRR6740495
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pyogenes
  ORGANISM  Streptococcus pyogenes
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 5012)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5012)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-OCT-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-OCT-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001918058.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001918058.2
            Genome Coverage       :: 368x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/12/2023 11:23:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 1,806
            CDSs (total)                      :: 1,769
            Genes (coding)                    :: 1,722
            CDSs (with protein)               :: 1,722
            Genes (RNA)                       :: 37
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1, 1 (5S, 23S)
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 47
            CDSs (without protein)            :: 47
            Pseudo Genes (ambiguous residues) :: 0 of 47
            Pseudo Genes (frameshifted)       :: 30 of 47
            Pseudo Genes (incomplete)         :: 20 of 47
            Pseudo Genes (internal stop)      :: 9 of 47
            Pseudo Genes (multiple problems)  :: 11 of 47
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5012
                     /organism="Streptococcus pyogenes"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN08555799-rid19682583.denovo.079"
                     /strain="MGAS11113"
                     /serotype="Emm28"
                     /isolation_source="normally sterile site"
                     /host="Homo sapiens"
                     /db_xref="taxon:1314"
                     /country="Finland"
                     /collection_date="2002"
                     /collected_by="National Public Health Laboratory"
     gene            248..1796
                     /locus_tag="VC122_001570"
     rRNA            248..1796
                     /locus_tag="VC122_001570"
                     /product="16S ribosomal RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00177"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00177"
     gene            1848..1920
                     /locus_tag="VC122_001571"
     tRNA            1848..1920
                     /locus_tag="VC122_001571"
                     /product="tRNA-Ala"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:1881..1883,aa:Ala,seq:tgc)
     gene            2221..>5012
                     /locus_tag="VC122_001572"
     rRNA            2221..>5012
                     /locus_tag="VC122_001572"
                     /product="23S ribosomal RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF02541"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF02541"
BASE COUNT         1426 a         1032 c         1444 g         1110 t
ORIGIN      
        1 gatatcgtat actaataaag ctgtcagaga ggttgctgta acacacttat tgaaaaaaga
       61 tgaaaaagta gttgacaggc tatgagaaac ctgatagaat aaagaagttg tctcttagga
      121 gacgttaaga cctttgagaa ctgaataaga cgaaccaaac gtgagggtga tatggagaca
      181 tattacccgt caagaaacga gaaataaatc tgtcagcgac agaaggaacg agtaagttca
      241 aacgcaaatt aaagagagtt tgatcctggc tcaggacgaa cgctggcggc gtgcctaata
      301 catgcaagta gaacgctgag aactggtgct tgcaccggtt caaggagttg cgaacgggtg
      361 agtaacgcgt aggtaaccta cctcatagcg ggggataact attggaaacg atagctaata
      421 ccgcataaga gagactaacg catgttagta atttaaaagg ggcaattgct ccactatgag
      481 atggacctgc gttgtattag ctagttggtg aggtaaaggc tcaccaaggc gacgatacat
      541 agccgacctg agagggtgat cggccacact gggactgaga cacggcccag actcctacgg
      601 gaggcagcag tagggaatct tcggcaatgg gggcaaccct gaccgagcaa cgccgcgtga
      661 gtgaagaagg ttttcggatc gtaaagctct gttgttagag aagaatgatg gtgggagtgg
      721 aaaatccacc aagtgacggt aactaaccag aaagggacgg ctaactacgt gccagcagcc
      781 gcggtaatac gtaggtcccg agcgttgtcc ggatttattg ggcgtaaagc gagcgcaggc
      841 ggttttttaa gtctgaagtt aaaggcattg gctcaaccaa tgtacgcttt ggaaactgga
      901 gaacttgagt gcagaagggg agagtggaat tccatgtgta gcggtgaaat gcgtagatat
      961 atggaggaac accggtggcg aaagcggctc tctggtctgt aactgacgct gaggctcgaa
     1021 agcgtgggga gcaaacagga ttagataccc tggtagtcca cgccgtaaac gatgagtgct
     1081 aggtgttagg ccctttccgg ggcttagtgc cggagctaac gcattaagca ctccgcctgg
     1141 ggagtacgac cgcaaggttg aaactcaaag gaattgacgg gggcccgcac aagcggtgga
     1201 gcatgtggtt taattcgaag caacgcgaag aaccttacca ggtcttgaca tcccgatgcc
     1261 cgctctagag atagagtttt acttcggtac atcggtgaca ggtggtgcat ggttgtcgtc
     1321 agctcgtgtc gtgagatgtt gggttaagtc ccgcaacgag cgcaacccct attgttagtt
     1381 gccatcatta agttgggcac tctagcgaga ctgccggtaa taaaccggag gaaggtgggg
     1441 atgacgtcaa atcatcatgc cccttatgac ctgggctaca cacgtgctac aatggttggt
     1501 acaacgagtc gcaagccggt gacggcaagc taatctctta aagccaatct cagttcggat
     1561 tgtaggctgc aactcgccta catgaagtcg gaatcgctag taatcgcgga tcagcacgcc
     1621 gcggtgaata cgttcccggg ccttgtacac accgcccgtc acaccacgag agtttgtaac
     1681 acccgaagtc ggtgaggtaa cctattagga gccagccgcc taaggtggga tagatgattg
     1741 gggtgaagtc gtaacaaggt agccgtatcg gaaggtgcgg ctggatcacc tcctttctaa
     1801 ggaaatggaa cacgtttatc gtcttattta gttttgagag gtcttgtggg gccttagctc
     1861 agctgggaga gcgcctgctt tgcacgcagg aggtcagcgg ttcgatcccg ctaggctcca
     1921 tcaggataca atcctactaa acttaataca agtgaagttg aacacgcaac tcacttccta
     1981 ggaaaataga caatcttccc ttgtgtgcaa ggcccacatg gtcagattcc taattttcta
     2041 cagaagtttc gctaaagcga gcgttgcttg gtatcctata taatagtcca ttgaaaattg
     2101 aatatctata tcaaattcca cgatctagaa atagattgta gaaaagtaac aagaaaataa
     2161 accgaaaacg ctgtgactat ttgataagtt ttctagtttt taaaaaaact aggttaataa
     2221 ggttaagtta ataagggcgc acggtggatg ccttggcact agaagccgaa gaaggacgtg
     2281 acaaacgacg aaatgctttg gggagctgta agtaagcgct gatccagaga tgtccgaatg
     2341 ggggaaccca ctaactaatg gttagtatcc ataactgtta aggttatgag aaggaagacg
     2401 cagtgaactg aaacatctaa gtagctgcag gaagagaaag caaacgcgat tgccttagta
     2461 gcggcgagcg aaacggcagg agggcaaacc gaagtgttta cacttcgggg ttgtaggact
     2521 gcgacgtggg actttaaaag gatagaagaa ttacctggga aggtaagcca aagagagtaa
     2581 aagcctcgta tttaaaattc ttttgagccc tagcagtatc ctgagtacgg cgagacacgt
     2641 gaaatctcgt tggaatctgg gaggaccatc tcccaaccct aaatactctc tagtgaccga
     2701 tagtgaacca gtaccgtgag ggaaaggtga aaagcacccc gggaggggag tgaaatagaa
     2761 cctgaaaccg tgtgcctaca acaagttcga gcccgttaat gggtgagagc gtgccttttg
     2821 tagaatgaac cggcgagtta cgatatgatg cgaggttaag ttgaagagac ggagccgtag
     2881 ggaaaccgag tcttaatagg gcgatttagt atcatgttgt agacccgaaa ccatgtgacc
     2941 tacccatgag caggttgaag gtgaggtaaa actcactgga ggaccgaacc agggcacgtt
     3001 gaaaagtgct tggatgactt gtgggtagcg gagaaattcc aaacgaactt ggagatagct
     3061 ggttctctcc gaaatagctt tagggctagc gtcgatgtta agtctcttgg aggtagagca
     3121 ctgtttgggt gaggggtcca tctcggatta ccaatctcag ataaactccg aatgccaacg
     3181 agatatcatc ggcagtcaga ctgcgagtgc taagatccgt agtcgaaagg gaaacagccc
     3241 agaccaccag ctaaggtccc aaaataactg ttaagtggaa aaggatgtgg ggttgcacag
     3301 acaactagga tgttagctta gaagcagcta ttcattcaaa gagtgcgtaa tagctcacta
     3361 gtcgagtgac cctgcgccga aaatgtaccg gggctaaaac agtttaccga agctgtggat
     3421 gacacaaaag tgtcatggta ggagagcgtt ctatgtgtga agaaggtgta ccgtgaggag
     3481 cgctggaacg catagaagtg agaatgccgg tatgagtagc gaaagacagg tgagaatcct
     3541 gtccaccgta agactaaggt ttccagggga aggctcgtcc gccctgggtt agtcgggacc
     3601 taaggagaga ccgaaaggtg tatccgatgg ccaacaggtt gatattcctg tactagagta
     3661 tatagtgatg gagggacgca gtaggctaac taaaccggac gattggaaga gtccggctaa
     3721 gcagtgaggt gtaagatgag tcaaatgctt atctttataa cattgagctg tgatggggag
     3781 cgaattttag tagcgaagtt agtgatgtca cactgccaag aaaagcttct agcgtttaat
     3841 gatactctac ccgtaccgca aaccgacaca ggtagtcgag gcgagtagcc tcaggtgatc
     3901 gagagaactc tcgttaagga actcggcaaa atgaccccgt aacttcggga gaaggggtgc
     3961 tgacttaggt cagccgcagt gaataggccc aagcaactgt ttatcaaaaa cacagctctc
     4021 tgctaaatcg taagatgatg tatagggggt gacgcctgcc cggtgctgga aggttaagag
     4081 gagggtttag cgcaagcgaa gatctgaatt gaagccccag taaacggcgg ccgtaactat
     4141 aacggtccta aggtagcgaa attccttgtc gggtaagttc cgacccgcac gaaaggcgta
     4201 atgatttggg cactgtctca acgagagact cggtgaaatt ttagtacctg tgaagatgca
     4261 ggttacccgc gacaggacgg aaagacccca tggagcttta ctgcagtttg atattgagta
     4321 tctgtaccac atgtacagga taggtaggag ccattgactt cgggacgcca gtttcgaatg
     4381 aggcgctgtt gggatactac ccttgtgtta tggctactct aacccagata ggttatccct
     4441 atcggagaca gtgtctgacg ggcagtttga ctggggcggt cgcctcctaa agagtaacgg
     4501 aggcgcccaa aggttccctc agattggttg gaaatcaatc gcagagtgta aaggtataag
     4561 ggagcttgac tgcgagagct acaactcgag cagggacgaa agtcgggctt agtgatccgg
     4621 tggtaccgaa tggaagggcc atcgctcaac ggataaaagc taccctgggg ataacaggct
     4681 tatctccccc aagagttcac atcgacgggg aggtttggca cctcgatgtc ggctcgtcgc
     4741 atcctggggc tgtagtcggt cccaagggtt gggctgttcg cccattaaag cggcacgcga
     4801 gctgggttca gaacgtcgtg agacagttcg gtccctatcc gtcgcgggcg taggaaattt
     4861 gagaggatct gctcctagta cgagaggacc agagtggact taccgctggt gtaccagttg
     4921 tcttgccaaa ggcatcgctg ggtagctatg tagggaaggg ataagcgctg aaagcatcta
     4981 agtgcgaagc ccccctcaag atgagatttc cc
//
LOCUS       DATXIG010000080         4925 bp    DNA     linear   BCT 28-DEC-2023
DEFINITION  TPA_asm: Streptococcus pyogenes strain MGAS11113
            SAMN08555799-rid19682583.denovo.080, whole genome shotgun sequence.
ACCESSION   DATXIG010000080 DATXIG010000000
VERSION     DATXIG010000080.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN08555799
            Sequence Read Archive: SRR6740495
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pyogenes
  ORGANISM  Streptococcus pyogenes
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 4925)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4925)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-OCT-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-OCT-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001918058.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001918058.2
            Genome Coverage       :: 368x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/12/2023 11:23:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 1,806
            CDSs (total)                      :: 1,769
            Genes (coding)                    :: 1,722
            CDSs (with protein)               :: 1,722
            Genes (RNA)                       :: 37
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1, 1 (5S, 23S)
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 47
            CDSs (without protein)            :: 47
            Pseudo Genes (ambiguous residues) :: 0 of 47
            Pseudo Genes (frameshifted)       :: 30 of 47
            Pseudo Genes (incomplete)         :: 20 of 47
            Pseudo Genes (internal stop)      :: 9 of 47
            Pseudo Genes (multiple problems)  :: 11 of 47
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4925
                     /organism="Streptococcus pyogenes"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN08555799-rid19682583.denovo.080"
                     /strain="MGAS11113"
                     /serotype="Emm28"
                     /isolation_source="normally sterile site"
                     /host="Homo sapiens"
                     /db_xref="taxon:1314"
                     /country="Finland"
                     /collection_date="2002"
                     /collected_by="National Public Health Laboratory"
     gene            <1..390
                     /gene="tilS"
                     /locus_tag="VC122_001573"
     CDS             <1..390
                     /gene="tilS"
                     /locus_tag="VC122_001573"
                     /EC_number="6.3.4.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011017224.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA lysidine(34) synthetase TilS"
                     /protein_id="HEP5641485.1"
                     /translation="KIVPKTELVKDEKMLEYDSNLCYRDYCFSFMPKSNEDQGQVSIP
                     LFSLSSIKLRSRQSGDYISFGHFSKKIRRLFIDEKFTIAERQNAIIGEQDEQIIFVLI
                     GNKTYLRKACKHDIMLAKLYIDKLEKG"
     gene            395..937
                     /gene="hpt"
                     /locus_tag="VC122_001574"
     CDS             395..937
                     /gene="hpt"
                     /locus_tag="VC122_001574"
                     /EC_number="2.4.2.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012961310.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypoxanthine phosphoribosyltransferase"
                     /protein_id="HEP5641486.1"
                     /translation="MLEQDIQKILYSENDIIRKTKKLGEQLTKDYQEKNPLMIGVLKG
                     SVPFMAELMKHIDTHVEIDFMVVSSYHGGTSSSGEVKILKDVDTNIEGRDIIIVEDII
                     DTGRTLKYLRDMFKYRKANTIKIATLFDKPEGRVVKIEADYVCYNIPNEFIVGFGLDY
                     AENYRNLPYVGVLKEEVYSK"
     gene            959..2938
                     /gene="ftsH"
                     /locus_tag="VC122_001575"
     CDS             959..2938
                     /gene="ftsH"
                     /locus_tag="VC122_001575"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002987683.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent zinc metalloprotease FtsH"
                     /protein_id="HEP5641487.1"
                     /translation="MKNNKNNGFVKNSFIYILMIIVVITGFQFYLKGTSTQSQQISYS
                     KLIKHLKAGDIKSLSYQPSGSIIEVKGKYEKPQKVTVNSGLSFLGGRASTQVTEFSSL
                     VLPSDTILKEMTAAADKNGTELTVKQESSSGTWITFLMSFLPIVIFAAFMMMMMNQGG
                     GGARGAMSFGKNKAKSQSKGDVKVRFTDVAGAEEEKQELVEVVDFLKNPKKYKSLGAR
                     IPAGVLLEGPPGTGKTLLAKAVAGEAGVPFFSISGSDFVEMFVGVGASRVRSLFEDAK
                     KAERAIIFIDEIDAVGRRRGAGMGGGNDEREQTLNQLLIEMDGFEGNENIIVIAATNR
                     SDVLDPALLRPGRFDRKVLVGRPDVKGREAILRVHAKNKPLANDVNLKVVAQQTPGFV
                     GADLENVLNEAALVAARRNKIKIDASDIDEAEDRVIAGPSKKDRTISQKEREMVAYHE
                     AGHTIVGLVLSNARVVHKVTIVPRGRAGGYMIALPKEDQMLLSKEDLKEQLAGLMGGR
                     VAEEIVFNAQTSGASNDFEQATQIARAMVTEYGMSEKLGPVQYEGNHAMMPGQISPEK
                     SYSAQTAQMIDDEVRELLNQARNQAADIINENRDTHKLIAEALLKYETLDAAQIKSIY
                     ETGKMPVDLETDDNEAHALSYDEIKNKMTESESAE"
     gene            3265..4656
                     /locus_tag="VC122_001576"
     CDS             3265..4656
                     /locus_tag="VC122_001576"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002987685.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HEP5641488.1"
                     /translation="MTIFRKKKKYSNKTEMQRHFKVIDLVFLGLGSMVGTGIFTVTGI
                     GAAKYAGPALTISIIISAIAIGILALFYAEFASRMPSNGGAYSYVYATLGEFPAWLVG
                     WYIIMEFLTAISSVAVGWGSYLKGLLANYNIVLPNALNGTFNLKNGTYIDILPVLVMF
                     FVTGIVLMNSKLALRFNSFLVILKFSALALFIFVGIFFIDHNNWSHFAPYGVGQITGG
                     KTGIFAGASVMFFAFLGFESISMAVDEVKEPQKTIPKGIILSLIIVTALYIVVTTILT
                     GIVHYTKLNVPDAVAFALRNIRLYWAADYVSIVAILTLITVCISMTYALARTIYSISR
                     DGLLPKSLYTLTKKNKVPQNATLVTGLLAMICAGIFPLSSLAEFVNICTLAYLIILSG
                     AIIKLRRIEGEPKANEFKTPLVPFLPMLAIIICLSFMSQYKAFTWIAFAIATIIGTLI
                     YLAYGYTHSIENK"
BASE COUNT         1662 a          701 c          940 g         1622 t
ORIGIN      
        1 aaaatcgttc ctaagactga gttagttaaa gatgaaaaaa tgttagaata cgatagtaac
       61 ttatgctaca gagattattg tttttcattt atgccaaaaa gtaacgaaga tcaaggacag
      121 gtaagtatac ctttatttag tttatcgtct attaaattaa gaagtcgcca atctggagat
      181 tatatttctt ttggtcactt ttcaaaaaaa ataagacgtt tatttataga tgaaaaattc
      241 acaatagctg aacgccaaaa tgctattatt ggagagcaag atgagcagat tatatttgta
      301 ttgataggta ataaaactta tttgagaaaa gcttgtaaac atgatataat gttggctaaa
      361 ctgtatattg ataaattaga aaaagggtga cagcatgctc gagcaagata ttcaaaaaat
      421 tctttactct gaaaatgata ttattcggaa aactaaaaaa ttaggagaac aattaacaaa
      481 agattatcaa gaaaaaaatc cgcttatgat tggtgtatta aaaggatcag ttccttttat
      541 ggcagaacta atgaaacaca tcgatactca tgttgaaatt gattttatgg tagtatcaag
      601 ttatcatggc ggtacatcta gcagtggtga agttaaaatt ttaaaagatg tagataccaa
      661 tattgaagga agagatatca taattgttga agatattatt gacaccggtc gcaccttaaa
      721 atatttacga gatatgttta agtaccgtaa agctaatact atcaaaattg caacactgtt
      781 tgataaacct gaaggtcgtg ttgttaaaat tgaagcagat tatgtttgtt acaatattcc
      841 aaatgaattt atcgtaggct ttggtttaga ttacgcagaa aattatcgca atcttcctta
      901 cgtaggtgta ttaaaagaag aagtctattc aaaatagaaa aaggtcaatg tttactgtat
      961 gaaaaataat aaaaataatg gttttgttaa aaattctttt atatatatat tgatgattat
     1021 tgttgtcatc acaggatttc aattttatct taagggaacc agcacacaaa gccaacagat
     1081 tagttattcc aaattaatta aacatcttaa ggctggagat ataaaatctt taagttatca
     1141 gcctagcggt agtattattg aagttaaagg gaaatatgag aaacctcaaa aagtgacagt
     1201 taattcagga ctatcatttt taggtggaag agcttcaaca caggtaacag agttctcatc
     1261 tttagtatta cctagtgata ccatcttgaa agaaatgaca gcagcagctg ataaaaatgg
     1321 aacagagctt acagttaagc aggaaagttc aagtggtact tggattacct tcttaatgag
     1381 ctttttacct attgttattt ttgctgcttt catgatgatg atgatgaatc aaggtggtgg
     1441 aggagctcgt ggtgctatga gtttcggcaa aaataaggca aaatctcaat caaaaggcga
     1501 tgttaaagtg agatttacag acgttgctgg tgccgaagaa gaaaaacaag aattagtaga
     1561 agttgttgat tttttgaaaa atcctaaaaa atataaatca ctaggagcac ggatcccagc
     1621 gggagttctt ctagaggggc ctccaggtac tggtaaaaca ttactagcta aagctgtagc
     1681 aggtgaagct ggagtgccat ttttcagtat ttcaggttca gattttgtcg aaatgtttgt
     1741 cggtgtaggt gctagtcgtg ttcgttcttt atttgaggat gctaaaaaag ctgagcgtgc
     1801 aatcatcttt atcgatgaaa ttgacgctgt aggtcgtcgt cgtggtgctg gcatgggagg
     1861 cggtaacgat gaacgagaac agacccttaa ccaattactc attgaaatgg atggttttga
     1921 gggtaatgag aacattatcg ttattgctgc aactaaccgt agtgatgttt tagatccagc
     1981 tttactaaga ccaggtcgtt ttgatcgtaa ggttttagtt ggtcgccctg atgttaaagg
     2041 acgtgaagcc attttacgtg ttcatgctaa aaataaacca cttgcaaatg acgttaattt
     2101 gaaagtagta gcccaacaga ctccaggttt tgtaggtgct gatcttgaaa atgttttaaa
     2161 tgaagctgcg ttggttgctg ctcgcagaaa taaaattaaa attgacgcta gtgatattga
     2221 tgaagctgaa gatcgtgtta ttgctggacc atcaaagaaa gatcgcacta tttcgcaaaa
     2281 agagcgagaa atggttgctt atcacgaagc tggtcatact attgttggtt tagttttatc
     2341 taatgcgcgt gtagttcaca aggtaactat tgtacctcgt ggacgagcag gtggttatat
     2401 gatcgctctt ccaaaagaag atcaaatgct tctttctaaa gaagatctga aagagcaatt
     2461 agcaggattg atgggtggtc gtgttgctga agaaattgtt tttaatgctc aaacatcagg
     2521 tgcgtcaaat gactttgagc aagctactca gatagcaagg gccatggtta cggaatatgg
     2581 tatgagtgaa aagcttggac cagttcaata tgaaggtaac catgcgatga tgccaggaca
     2641 gatctctcct gaaaaatctt actctgccca aacagctcaa atgattgatg atgaggttcg
     2701 tgaactctta aatcaagctc gtaatcaagc agctgatatc atcaatgaga atcgagatac
     2761 tcataaattg attgctgaag ctcttctcaa atatgaaact ctagacgctg cacaaattaa
     2821 atcaatttat gaaactggta aaatgccagt tgatttagaa actgatgata atgaagctca
     2881 tgcgttatct tatgacgaaa ttaaaaataa aatgacagaa tctgaatctg cagaatgata
     2941 taaaaaagct gaacttatag attaagttca gcttttttat caacacaata tccttgtaaa
     3001 cgttatgaat tgtaataggt gcctaattgc tagaaatgag aagatgacat actttttttt
     3061 ctttattact taaagtgaga aagactttgg tatttaaaac tagaaaattg ttaaagccat
     3121 ttgaaaaaat ctattagtgt tttcttagaa taatagaaga acagaagata gcaaaagttt
     3181 cagtcaacca ataagatgaa atttacaatc attactaact atgatacact tagtagaaaa
     3241 tcatttacta tagaaggtga taacatgact atttttcgta aaaaaaagaa atattccaat
     3301 aaaacagaaa tgcaaaggca cttcaaagtt attgatttag tttttttagg cctaggttca
     3361 atggttggaa caggtatctt tacagttact ggcataggcg ctgctaagta tgcaggtcct
     3421 gctttaacaa tatcgattat catttccgcg attgctattg gaattttagc cttattttat
     3481 gcagaatttg cgtctcgtat gccttctaac ggtggtgctt atagttatgt ttatgcaacc
     3541 ttgggagagt ttccagcatg gcttgttggc tggtatatta ttatggaatt tttaacagct
     3601 atatctagtg ttgctgttgg ctggggaagt tatttaaaag gattacttgc caattataat
     3661 atagtattac caaacgcttt aaatggcacg ttcaatctta agaatggaac ttacatagat
     3721 attcttcctg ttttagtgat gtttttcgtg acagggattg tcctaatgaa ttcgaaatta
     3781 gccctgagat tcaatagttt tttagttatt ttaaaatttt cagcactggc tttatttatc
     3841 tttgtaggca ttttttttat tgatcacaat aattggtctc attttgctcc atatggtgta
     3901 ggacaaatta ctggaggtaa aacaggtatc tttgctggtg cttctgttat gttctttgca
     3961 tttttaggat ttgaatctat ttcaatggca gttgatgaag tgaaagaacc acaaaaaaca
     4021 attcctaaag gtatcatttt atctttgatt attgtaacag cactctatat agttgtcacc
     4081 actattttaa cgggaattgt acactacact aaattaaatg ttccagacgc ggtggctttt
     4141 gctttaagaa atattagact ttattgggca gctgactatg tgtcgatagt tgctattttg
     4201 actcttatta cggtatgtat ttctatgacc tatgcattag ctagaacaat ttacagtatt
     4261 agtagagatg gtttattacc taaatcactt tatactctca ctaaaaaaaa taaagtgcct
     4321 caaaatgcta ctcttgtcac tggactactt gctatgattt gtgcgggaat atttccttta
     4381 tcgagtctag cagagtttgt taatatttgt actttagcat atttaattat tctgtcgggt
     4441 gcaattatta aactcagaag aatagaagga gaaccaaaag caaatgaatt taaaactcct
     4501 ctagtaccat tcctaccaat gttggcaatt atcatttgtc tatcatttat gagccaatat
     4561 aaagctttta cttggattgc ttttgctatt gccacaatta ttgggacatt aatctactta
     4621 gcttatggtt atactcattc aatagaaaat aaataatgtt ttgataataa gatttgacat
     4681 tacattatag actataatgt ttagaagtaa atgagtagta aaatcaatac ctacttactt
     4741 atcaaagttg taatatttaa aattacccaa cccctagaga attgattagt ctctaggggt
     4801 tgattggtac aatgacatag actctaaaac gattttaata ctaaatatga tagatataac
     4861 tttcaaaaaa gaaaattagt tcatttttgt tacacaataa aatttgttat ttagcagtaa
     4921 tataa
//
LOCUS       DATXIG010000081         4856 bp    DNA     linear   BCT 28-DEC-2023
DEFINITION  TPA_asm: Streptococcus pyogenes strain MGAS11113
            SAMN08555799-rid19682583.denovo.081, whole genome shotgun sequence.
ACCESSION   DATXIG010000081 DATXIG010000000
VERSION     DATXIG010000081.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN08555799
            Sequence Read Archive: SRR6740495
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pyogenes
  ORGANISM  Streptococcus pyogenes
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 4856)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4856)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-OCT-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-OCT-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001918058.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001918058.2
            Genome Coverage       :: 368x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/12/2023 11:23:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 1,806
            CDSs (total)                      :: 1,769
            Genes (coding)                    :: 1,722
            CDSs (with protein)               :: 1,722
            Genes (RNA)                       :: 37
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1, 1 (5S, 23S)
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 47
            CDSs (without protein)            :: 47
            Pseudo Genes (ambiguous residues) :: 0 of 47
            Pseudo Genes (frameshifted)       :: 30 of 47
            Pseudo Genes (incomplete)         :: 20 of 47
            Pseudo Genes (internal stop)      :: 9 of 47
            Pseudo Genes (multiple problems)  :: 11 of 47
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4856
                     /organism="Streptococcus pyogenes"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN08555799-rid19682583.denovo.081"
                     /strain="MGAS11113"
                     /serotype="Emm28"
                     /isolation_source="normally sterile site"
                     /host="Homo sapiens"
                     /db_xref="taxon:1314"
                     /country="Finland"
                     /collection_date="2002"
                     /collected_by="National Public Health Laboratory"
     gene            88..1902
                     /gene="glmS"
                     /locus_tag="VC122_001577"
     CDS             88..1902
                     /gene="glmS"
                     /locus_tag="VC122_001577"
                     /EC_number="2.6.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019804802.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine--fructose-6-phosphate transaminase
                     (isomerizing)"
                     /protein_id="HEP5641489.1"
                     /translation="MCGIVGVVGNRNATDILMQGLEKLEYRGYDSAGIFVANANQTNL
                     IKSVGRIADLRAKIGIDVAGSTGIGHTRWATHGQSTEDNAHPHTSQTGRFVLVHNGVI
                     ENYLHIKTEFLAGHDFKGQTDTEIAVHLIGKFVEEDKLSVLEAFKKALSIIEGSYAFA
                     LMDSQATDTIYVAKNKSPLLIGLGEGYNMVCSDAMAMIRETSEFMEIHDKELVILTKD
                     KVTVTDYDGKELIRDSYTAELDLSDIGKGTYPFYMLKEIDEQPTVMRQLISTYADETG
                     NVQVDPAIITSIQEADRLYILAAGTSYHAGFATKNMLEQLTDTPVELGVASEWGYHMP
                     LLSKKPMFILLSQSGETADSRQVLVKANAMGIPSLTVTNVPGSTLSREATYTMLIHAG
                     PEIAVASTKAYTAQIAALAFLAKAVGEANGKQEALDFNLVHELSLVAQSIEATLSEKD
                     LVAEKVQALLATTRNAFYIGRGNDYYVAMEAALKLKEISYIQCEGFAAGELKHGTISL
                     IEEDTPVIALISSSQLVASHTRGNIQEVAARGAHVLTVVEEGLDREGDDIIVNKVHPF
                     LAPIAMVIPTQLIAYYASLQRGLDVDKPRNLAKAVTVE"
     gene            2098..2433
                     /locus_tag="VC122_001578"
     CDS             2098..2433
                     /locus_tag="VC122_001578"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011017832.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alkylphosphonate utilization protein"
                     /protein_id="HEP5641490.1"
                     /translation="MSLPNCLQCQSEYVYEDGLLLICPMCAFEWTPGEEATEEEGLVV
                     LDSNGVRLSDGDTITVVKDLKVKGAPKDLKQGTRVKNIRLVEGDHNIDCKIDGFGAMK
                     LKSEFVKKI"
     gene            2540..3181
                     /locus_tag="VC122_001579"
     CDS             2540..3181
                     /locus_tag="VC122_001579"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009854101.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter permease"
                     /protein_id="HEP5641491.1"
                     /translation="MTYIQQVLPSLLDGALVTLQVFFIVIILSIPLGAILAFLMKIPF
                     KPLQWFLTLYVWMMRGTPLLLQLIFFYYVLPSVGISFDRMPAAILAFTLNYAAYFAEI
                     FRGGIEAIPKGQYEAAKVLKLKPLQTIRYIILPQVFKIVLPSVFNEVINLVKDSSLVY
                     VLGVGDLLLASKTAANRDATLAPMFIAGLIYLLLIGLVTIISKQVEKRFNYYQ"
     gene            3191..3820
                     /locus_tag="VC122_001580"
     CDS             3191..3820
                     /locus_tag="VC122_001580"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009854102.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter ATP-binding protein"
                     /protein_id="HEP5641492.1"
                     /translation="MLELKNISKQFGQKTIFDGFNLTVQDGEVLSLVGPSGGGKTTLL
                     RMLAGLESIDSGQVFYNGEDVGIDHLENRNLLGFVFQDFQLFPHLTVLDNLTLSPTIT
                     MGKQKSDAKEKALDLLARLGLKEHAQVYPYSLSGGQKQRVALARAMMIDPQIIGYDEP
                     TSALDPELRQTVEALIVQNREMGITQIVVTHDLVFAEAISDRIIRVNPK"
     gene            3836..4672
                     /locus_tag="VC122_001581"
     CDS             3836..4672
                     /locus_tag="VC122_001581"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014635494.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter substrate-binding
                     protein"
                     /protein_id="HEP5641493.1"
                     /translation="MIIKKRTVAILAIASSFFLVACQATKGLKSGDAWGVYQKQKSIT
                     VGFDNTFVPMGYKDESGKCKGFDIDLAKEVFHQYGLKVNFQAINWDMKEAELNNGKID
                     VIWNGYSITKERQEKVAFTDSYMRNEQIIVVKKRSDIKTISDMKHKVLGAQSASSGYD
                     SLLRTPKLLKDFIKNKDANQYETFTQAFIDLKSDRIDGILIDKVYANYYLAKEGQLEN
                     YRMIPTTFENEAFSVGLRKEDKTLQAKINRAFRVLYQNGKFQAISEKWFGDDVATANI
                     KS"
BASE COUNT         1510 a          794 c         1025 g         1527 t
ORIGIN      
        1 ctgtttttat cggtataata tgactatacc aattattgaa attatgagag aaagtagcaa
       61 gttaacgaac ttgttaaggt aattaagatg tgtggaattg ttggagttgt tggaaatcgc
      121 aatgcaacgg atattttaat gcaaggcctt gaaaagcttg aataccgggg ttatgattca
      181 gcaggaattt ttgtggctaa tgccaatcaa acaaacttga ttaaatcagt ggggcggatt
      241 gctgatttgc gtgccaagat tggcattgat gttgctggtt caacagggat tggtcacacc
      301 cgttgggcaa cgcatggcca atcaacagag gataatgccc atcctcacac gtcacaaact
      361 ggacgttttg tacttgttca taatggtgtg attgaaaatt accttcacat taaaacagag
      421 ttcctagctg gacatgattt taaggggcag acagatactg agattgcagt acacttgatt
      481 ggaaaatttg tggaagaaga caagttgtca gtactggaag cttttaaaaa agctttaagc
      541 attattgaag gttcctacgc ctttgcgtta atggatagcc aagcaactga tactatttat
      601 gtggctaaaa acaagtctcc attgttgatt ggacttggtg aaggttacaa catggtttgt
      661 tcagatgcca tggccatgat tcgtgaaacc agtgaattta tggaaattca tgataaggag
      721 ctagttattt taaccaaaga taaggtaact gttacagact acgatggtaa agagctgata
      781 cgagattcct acactgctga attagactta tctgatattg gcaaagggac ttatcctttc
      841 tatatgctga aagaaattga tgagcaacca accgtaatgc gtcaattaat ttcaacttat
      901 gcagatgaaa ctggtaacgt acaggttgat ccagctatca ttacctctat ccaagaggct
      961 gaccgtcttt atattttagc ggcagggact tcctaccatg ctggttttgc aacaaaaaat
     1021 atgcttgagc aattgacaga tacaccagtt gagttgggag tggcttctga gtggggttac
     1081 cacatgcctc tgcttagcaa gaaaccaatg tttattctac taagccaatc aggagaaacc
     1141 gcagatagtc gtcaagtttt agtaaaggca aatgctatgg gtattccgag tttgacagta
     1201 actaacgttc caggatcaac cttatcacgt gaagcaacat acaccatgtt gattcatgct
     1261 ggacctgaaa ttgctgttgc gtctacaaaa gcttacactg cacaaattgc tgcccttgcc
     1321 tttttggcta aggcagttgg tgaggcaaat ggtaagcaag aagctcttga ctttaacttg
     1381 gtacatgagt tgtcattggt tgcccaatct attgaggcga ctttgtctga aaaagatctc
     1441 gtggcagaaa aggttcaagc tttgctagct actactcgta atgcttttta cattgggcgt
     1501 ggcaatgatt attacgttgc gatggaagct gctttgaaat taaaagagat ttcttatatt
     1561 caatgcgaag gctttgcggc tggtgaattg aaacatggaa ccatttcatt gattgaggag
     1621 gacacgccag taatcgcttt aatatcgtct agtcagttgg ttgcctctca tacgcgtggt
     1681 aatattcaag aagttgctgc ccgtggggct catgttttaa cagttgtgga agaagggctt
     1741 gaccgtgagg gagatgacat tattgtcaat aaggttcatc ctttcctagc cccgattgct
     1801 atggtcattc caactcaact gattgcttac tacgcttcat tacaacgtgg acttgatgtt
     1861 gataagccac gtaatttggc taaagctgta acagtagaat aaatgattat aatggataag
     1921 gcaaccatct gttaggtggt tgctttttgt ttttaggaga atagcagagc ttagaaaatg
     1981 gccttgagaa aagctataca agaagaatag tcatcttctg atgagaacgt attcatcttg
     2041 tcaaatcatt gattctaaag taaaatggta acattaagaa atgtttagga gtgtaagatg
     2101 tcattaccaa attgtttgca atgccagtct gaatatgttt acgaagatgg tcttttatta
     2161 atctgtccaa tgtgtgcttt tgaatggaca ccaggcgaag aagcgactga agaagaagga
     2221 cttgttgttt tagatagcaa tggtgtccga ctgtctgatg gtgataccat tactgtggtt
     2281 aaagatttga aagtaaaagg cgctccaaaa gacctgaaac aagggactcg tgttaaaaat
     2341 attcgcctgg tggaaggtga tcataacatt gattgtaaga ttgatgggtt tggcgcgatg
     2401 aaattgaaat cagaatttgt caagaaaatc taataataat acgcaaccag ctgatctggt
     2461 tgtattttta taaaaaaacc tctccccaaa tacgttcaaa tttggtataa tagtaaaata
     2521 ttcagtttta ggaggcaaca tgacctatat tcagcaagtt ttacccagct tattagatgg
     2581 tgccttggtg accttacaag tattctttat tgttatcatt ctttctatac ccttaggggc
     2641 tattttagct ttcttgatga agattccctt taaaccgcta cagtggtttt tgaccctata
     2701 cgtgtggatg atgcgaggga caccattact acttcaattg atttttttct attatgtttt
     2761 gccaagtgtg gggattagtt ttgatcgaat gccagctgct attttggcgt ttactttgaa
     2821 ttatgctgcc tactttgctg aaatttttag aggtgggatt gaggctattc caaaaggtca
     2881 atatgaagca gctaaagtat taaagttaaa acctcttcaa accattcgtt atattatttt
     2941 gcctcaagtg tttaaaattg tgttaccaag tgttttcaat gaagtcatta atttggtcaa
     3001 agattcttcc cttgtctatg tactcggtgt aggagatctt ttattagcaa gtaagacggc
     3061 agccaatagg gatgcaacct tagcccctat gtttatcgct ggtcttatct atttgctttt
     3121 aattggactg gtcacgatta tttcaaaaca agttgaaaaa cggtttaatt attatcagta
     3181 aggaggcttc atgttagaac tcaaaaatat ttccaaacag tttggtcaaa aaaccatttt
     3241 tgatggcttt aatctaaccg ttcaagacgg agaagtgctc tctttggtag gcccatcagg
     3301 tggcggtaag acaacactct tacggatgtt ggctggtctt gaatctattg attcaggtca
     3361 agtcttttac aatggcgaag atgttggtat tgatcatttg gaaaatcgta acttgcttgg
     3421 atttgttttt caagattttc aattgtttcc tcatcttacc gttttagaca acctaacgct
     3481 gtctccaaca attacaatgg gcaagcaaaa atctgacgct aaagaaaagg ctttggattt
     3541 actggcacgt ttaggattaa aagaacatgc ccaagtttat ccctattcgt tatctggagg
     3601 tcaaaaacaa cgggttgctt tggcaagagc catgatgatt gaccctcaaa ttatcggtta
     3661 cgatgaaccg accagtgctc tagatcctga attacgtcag acggtagaag ccttgattgt
     3721 tcaaaaccgt gaaatgggaa taactcaaat tgtagtcact cacgatcttg tttttgccga
     3781 agccatctca gatcgtatta ttagggttaa tcctaaatag aagaggaccc aaaagatgat
     3841 tataaaaaaa agaaccgtag caattttagc catagctagt agctttttct tggtagcttg
     3901 tcaagctact aaaggtctta aatcaggaga tgcttgggga gtttaccaaa agcaaaaaag
     3961 tattacagtt ggttttgaca atacgtttgt tcctatgggc tataaggatg aaagcggcaa
     4021 atgcaaaggt tttgatattg atttggctaa agaagttttt caccaatatg gactcaaggt
     4081 taactttcaa gctattaatt gggacatgaa agaagcagaa ctaaacaatg gtaaaattga
     4141 tgtaatctgg aatggttatt caataactaa ggagcgtcag gaaaaggttg cctttactga
     4201 ttcttacatg agaaatgaac aaattattgt tgtcaaaaaa agatctgata ttaaaacaat
     4261 atcagatatg aaacataaag tgttaggagc acaatcagct tcatcaggtt atgactcctt
     4321 gttaagaact cctaaactgc tgaaagattt tattaaaaat aaagacgcta atcaatatga
     4381 aacctttaca caagctttta ttgatttaaa atcagatcgt atcgatggaa tattgattga
     4441 caaagtatat gccaattact atttagcaaa agaagggcaa ttggagaatt atcggatgat
     4501 cccaacgacc tttgaaaatg aagcattttc ggttggactt agaaaagaag acaaaacgtt
     4561 gcaagcaaaa attaatcgtg ctttcagggt gctttatcaa aatggcaaat ttcaagctat
     4621 ttctgagaaa tggtttggag atgatgttgc cactgccaat attaaatctt aaaaaggaca
     4681 cttgtccttt ttttgtttaa caggtgagat ctagcagaca aagtcagcta gatgatttaa
     4741 taaaaatctt agtttaaggt ttttttatta aactgtattt tttctaaaaa ttatagcttt
     4801 tttagactga taaatgacaa gaaaagttat tttaggtcaa ttaacgtccg tttaaa
//
LOCUS       DATXIG010000082         4758 bp    DNA     linear   BCT 28-DEC-2023
DEFINITION  TPA_asm: Streptococcus pyogenes strain MGAS11113
            SAMN08555799-rid19682583.denovo.082, whole genome shotgun sequence.
ACCESSION   DATXIG010000082 DATXIG010000000
VERSION     DATXIG010000082.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN08555799
            Sequence Read Archive: SRR6740495
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pyogenes
  ORGANISM  Streptococcus pyogenes
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 4758)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4758)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-OCT-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-OCT-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001918058.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001918058.2
            Genome Coverage       :: 368x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/12/2023 11:23:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 1,806
            CDSs (total)                      :: 1,769
            Genes (coding)                    :: 1,722
            CDSs (with protein)               :: 1,722
            Genes (RNA)                       :: 37
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1, 1 (5S, 23S)
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 47
            CDSs (without protein)            :: 47
            Pseudo Genes (ambiguous residues) :: 0 of 47
            Pseudo Genes (frameshifted)       :: 30 of 47
            Pseudo Genes (incomplete)         :: 20 of 47
            Pseudo Genes (internal stop)      :: 9 of 47
            Pseudo Genes (multiple problems)  :: 11 of 47
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4758
                     /organism="Streptococcus pyogenes"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN08555799-rid19682583.denovo.082"
                     /strain="MGAS11113"
                     /serotype="Emm28"
                     /isolation_source="normally sterile site"
                     /host="Homo sapiens"
                     /db_xref="taxon:1314"
                     /country="Finland"
                     /collection_date="2002"
                     /collected_by="National Public Health Laboratory"
     gene            20..1216
                     /locus_tag="VC122_001582"
     CDS             20..1216
                     /locus_tag="VC122_001582"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002996017.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CHAP domain-containing protein"
                     /protein_id="HEP5641494.1"
                     /translation="MKKRILSAVLVSGVTLGAATTVGAEDLSTKIAKQDSIISNLTTE
                     QKAAQNQVSALQAQVSSLQSEQDKLTARNTELEALSKRFEQEIKALTSQIVARNEKLK
                     NQARSAYKNNETSGYINALLNSKSISDVVNRLVAINRAVSANAKLLEQQKADKVSLEE
                     KQAANQTAINTIAANMAMAEENQNTLRTQQANLVAATANLALQLASATEDKANLVAQK
                     EAAEKAAAEALAQEQAAKVKAQEQAAQQAASVEAAKSAITPAPQATPAAQSSNAIEPA
                     ALTAPAAPSAGPQTSYDSSNTYPVGQCTWGAKSLAPWAGNNWGNGGQWAYSAQAAGYR
                     TGSTPMVGAIAVWNDGGYGHVAVVVEVQSASSIRVMESNYSGRQYIADHRGWFNPTGV
                     TFIYPH"
     gene            1469..2431
                     /locus_tag="VC122_001583"
     CDS             1469..2431
                     /locus_tag="VC122_001583"
                     /EC_number="2.7.6.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002986722.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribose-phosphate diphosphokinase"
                     /protein_id="HEP5641495.1"
                     /translation="MSYSDLKLFALSSNKELAEKVASAMDLQLGKSTVRQFSDGEIQV
                     NIEESIRGHHVFILQSTSSPVNDNLMEILIMVDALKRASAEKISVVMPYYGYARQDRK
                     ARSREPITSKLVANMLEVAGVDRLLTVDLHAAQIQGFFDIPVDHLMGAPLIADYFDRH
                     GLVGEDVVVVSPDHGGVTRARKLAQFLQTPIAIIDKRRSVDKMNTSEVMNIIGNVSGK
                     KCILIDDMIDTAGTICHAADALAEAGATAVYASCTHPVLSGPALDNIQRSAIEKLIVL
                     DTIYLPKERLIDKIEQISIADLVAEAIIRIHEKRPLSPLFEMGN"
     gene            2617..3372
                     /gene="recO"
                     /locus_tag="VC122_001584"
     CDS             2617..3372
                     /gene="recO"
                     /locus_tag="VC122_001584"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002986719.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein RecO"
                     /protein_id="HEP5641496.1"
                     /translation="MQLTESLGIVLFNRNYREDDKLVKIFTEVAGKQMFFVKHISRSK
                     MSSIIQPLTIADFIFKLNDTGLSYVVDYSNVNTYRYINNDIFRLAYASYVLALADAAI
                     ADNESDSHLFTFLKKTLDLMEEGLDYEILTNIFEIQILDRFGISLNFHECAICHRTDL
                     PLDFSHRFSAVLCSEHYYKDNRRNHLDPNVIYLLSRFQKITFDDLRTISLNKDIKKKL
                     RQFIDELYHDYVGIKLKSKTFIDNLVKWGDIMK"
     gene            3475..4482
                     /gene="plsX"
                     /locus_tag="VC122_001585"
     CDS             3475..4482
                     /gene="plsX"
                     /locus_tag="VC122_001585"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012514701.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate acyltransferase PlsX"
                     /protein_id="HEP5641497.1"
                     /translation="MKRIAIDAMGGDNAPKAIVEGVNQAIEAFSDIEIQLYGDQTKIN
                     SYLIQSDRVAIIHTDEKIMSDDEPAKAVRRKKKASMVLAAKAVKEGKADAIISAGNTG
                     ALLAVGLFVVGRIKGVDRPGLLSTIPTVTGLGFDMLDLGANAENTAKHLHQYAILGSF
                     YAKNVRGIANPRVGLLNNGTEETKGDPLRKATYELLTADNTISFVGNVEARELMSGVA
                     DVIVSDGFTGNAVLKSIEGTAISIMGQLKQIINSGGIKTKIGASLLKSSLYEMRKTLD
                     YSSAGGAVLFGLKAPVVKSHGSSDVKAIFSTIKQVRTMLDTNVVGQLVEEFAKETQVN
                     D"
     gene            4475..4717
                     /locus_tag="VC122_001586"
     CDS             4475..4717
                     /locus_tag="VC122_001586"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011017228.1"
                     /note="carries the fatty acid chain in fatty acid
                     biosynthesis; Derived by automated computational analysis
                     using gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl carrier protein"
                     /protein_id="HEP5641498.1"
                     /translation="MTRQEIFERLINLIQKQRSYLSVAITEQTHLKNDLAVDSIELVE
                     FIINVEDEFHLAIPDEDVEDMVFMGDVLDYLVQRLT"
BASE COUNT         1540 a          742 c          986 g         1490 t
ORIGIN      
        1 aaggagtagt ttttttatta tgaaaaaaag aattttatca gcagttcttg taagtggtgt
       61 taccctcgga gcagctacaa ctgtaggagc ggaggattta agtactaaga ttgctaagca
      121 agattctatt atctcaaatc tgactacaga gcaaaaagct gcacagaatc aagtttcagc
      181 gttacaggct caagtaagtt cactacaatc tgaacaagat aaactgaccg caagaaatac
      241 agaacttgag gcgctttcaa agcgatttga gcaagaaatt aaggctctaa caagtcaaat
      301 tgttgctcgt aatgaaaaat taaaaaatca agctcgtagt gcttataaaa acaatgaaac
      361 ttctggttat attaatgcac ttttgaattc taaatcaatt tctgatgttg taaaccgttt
      421 agtagcaatt aatagagctg tctctgctaa cgctaaattg ttagaacaac aaaaagctga
      481 taaagtttcc cttgaagaaa agcaagctgc taaccaaaca gctattaata ccattgccgc
      541 taatatggca atggctgaag aaaaccaaaa tacattacgt actcaacaag ctaatttggt
      601 agctgcaact gcaaatttag ctctccaatt agcatctgct actgaagata aagctaattt
      661 ggtagctcaa aaagaagctg cagaaaaagc tgctgctgaa gccttagcac aagaacaggc
      721 tgctaaagtt aaggcacaag aacaggctgc acaacaagca gcatctgttg aagcagcaaa
      781 atctgctatt actccagcac cacaagctac tccggcagcg caaagtagta atgctattga
      841 accagctgca ctcacggctc cggcagctcc ttctgcagga ccacaaacat catatgattc
      901 ttctaatact tatccagttg gacaatgcac atggggagct aaatctttag ctccttgggc
      961 aggaaataat tggggaaatg gtggtcaatg ggcttatagt gctcaagcag ctggttatcg
     1021 tactggttca acgccgatgg taggtgcgat tgccgtttgg aacgatggtg gttatggaca
     1081 tgtcgccgtt gtagttgagg ttcaaagtgc ctcaagtatt cgtgtgatgg agtctaacta
     1141 cagtggtaga cagtacattg ctgaccaccg tggctggttt aatccaacag gtgttacatt
     1201 tatttatcca cactaacata ttatttaggt tagaacagtt gttctaacct ttttgattac
     1261 tcctattgtg gaatagttct gagagtattg acgatatagt taatttataa gagagttgtt
     1321 tggccaatcg cttatgtata atataagaga agtatataaa gtcgataaaa ggtatattta
     1381 atacattctt taacggtttt tcaatttcat ttgataattc acttgaaaag cgttaaaatg
     1441 gtataggatt aaaactttgg aggaaaaaat gtcttattct gatttaaaat tgtttgcttt
     1501 atcgtcaaac aaggaattag cagaaaaagt tgcgtctgca atggatcttc aacttggaaa
     1561 gtctacggtt cgccagtttt cagatggtga aatccaagta aatattgaag aatcaattcg
     1621 tggtcatcat gtctttattt tgcaatcaac tagctcacct gtcaatgaca atttaatgga
     1681 aattttgatt atggtcgatg ctttaaagcg tgctagtgct gaaaaaattt cagttgtgat
     1741 gccttactac ggctatgcac gccaagaccg taaagcgcgt tctcgtgaac ctattacttc
     1801 gaaattagtt gctaatatgt tagaagttgc tggtgtagat cgcttattaa cggttgattt
     1861 acatgctgct caaattcaag ggttctttga tattccagtt gatcacttga tgggtgcacc
     1921 tttaattgca gactattttg atcgtcatgg tttagtgggt gaagatgttg tcgttgttag
     1981 tcctgaccat ggtggtgtga ctcgtgcgcg taaattagca caattcctcc aaacaccaat
     2041 tgcaattatc gataagcgtc gtagtgtgga taagatgaac accagtgaag tgatgaatat
     2101 tatcggaaat gtttcgggca agaaatgtat cttgattgac gatatgattg ataccgcagg
     2161 gactatttgc catgcagcag atgcgcttgc tgaagcgggg gcaacagctg tgtatgcgtc
     2221 atgtacgcat cccgttcttt ctggaccagc acttgataat attcaacgtt cggctatcga
     2281 aaaactaatt gttcttgata ctatttatct gccaaaagaa cgccttattg ataaaattga
     2341 acaaatttca attgctgatt tggttgcaga agcgattatt cgcattcatg aaaaacgtcc
     2401 gttgtcacct ttatttgaga tgggcaatta aacttaacga agcctgagat aatagatctc
     2461 aggctttttg acttgaataa aatgtaaggg taatgtggta ttaattattg tgggatgaac
     2521 tatgcaattc taaaggattt tgggtataat aagaaagata acaacagttt attgtaaact
     2581 aagataaaag gtaagagaaa aatacgacga ggtaatatgc aactaacaga atcactaggc
     2641 atcgttcttt ttaataggaa ttatcgagaa gatgataaat tagtcaaaat atttactgaa
     2701 gtagcaggta agcagatgtt tttcgtgaaa catattagtc gttccaaaat gtcctcaatc
     2761 attcaaccac taacgattgc tgattttatt ttcaagttaa atgatacagg cttgtcttat
     2821 gttgttgact atagtaacgt taacacttat cggtatatta ataatgatat ttttcgatta
     2881 gcctatgcta gttatgtctt agcattagct gatgctgcga ttgcagataa tgaatcagat
     2941 tcgcatttgt ttacgttttt gaaaaaaaca cttgatttga tggaagaggg cctagattat
     3001 gaaattttga caaatatttt tgaaattcag atattagatc gttttggtat tagtctaaac
     3061 tttcatgagt gtgccatttg tcatcgcact gatttaccgc ttgatttttc ccatcgtttt
     3121 tcagctgtac tttgttctga acattattac aaagacaacc gacgtaatca tttagatcca
     3181 aatgttatct acttgttgag tcgatttcaa aaaatcacat ttgatgattt gagaactatt
     3241 tcattgaata aagacatcaa aaagaagctt cgtcagttca ttgatgagtt gtatcacgac
     3301 tatgtaggaa tcaaattaaa aagtaaaaca ttcattgata atttagttaa gtggggagat
     3361 attatgaaat gatagggcct ctttcgttta tgggaattgg tttcagaata tgctataata
     3421 aaaatactat taatagattg tgtaagaaca caagatataa ttaaaggagt ggaaatgaag
     3481 agaattgcaa tagatgctat gggtggagat aacgctccta aagctattgt tgagggtgtt
     3541 aatcaagcaa tagaagcatt ttcagatatc gaaattcaac tttacggtga tcagacaaag
     3601 attaactcct atcttattca atcagatcgt gttgctatta ttcatacaga tgaaaaaatt
     3661 atgtcagatg atgaacctgc aaaagctgtt cgccgtaaaa agaaggcttc aatggtttta
     3721 gcagctaagg cagtgaaaga aggaaaagca gatgccatta tttcagcagg taatacaggt
     3781 gctcttttag ctgtaggttt gtttgttgtg gggcgtatca agggagttga tcgtccagga
     3841 ttattatcaa ctattcccac agtaaccgga ttaggatttg atatgttgga tcttggagct
     3901 aatgctgaaa atacggcaaa acatttacat caatatgcta ttttaggctc tttctatgct
     3961 aagaatgtac gtgggattgc taatccacgt gttggtctat taaacaatgg tacagaagaa
     4021 acaaaaggag atccccttcg taaggccact tatgaacttt taacagcaga taatacaata
     4081 tcttttgttg gtaatgtcga ggcgcgagaa cttatgtcag gtgttgcaga tgttattgta
     4141 tcagatggtt tcacgggaaa cgcagtgttg aaatcaatag aaggaacagc tattagcatt
     4201 atggggcaac tgaaacagat aattaatagc ggcggtatta agactaaaat tggagctagt
     4261 ctacttaaaa gtagtcttta cgagatgagg aagactcttg actactctag tgctggaggt
     4321 gcagttttat ttggtttaaa ggcacctgtt gtaaagtctc atggttctag tgatgtaaaa
     4381 gctatttttt caacgataaa acaagtacgt acgatgctag acactaacgt ggtcggacaa
     4441 ttagtagaag aatttgctaa ggagactcaa gtaaatgact agacaggaga tttttgaaag
     4501 actgattaac ttaattcaga agcaaagaag ctatttgagt gtagctatta cagaacaaac
     4561 acatttaaaa aatgatttag cggttgactc tattgaacta gttgaattta ttattaatgt
     4621 tgaagatgaa tttcatcttg ctattcctga tgaagatgtt gaggatatgg tatttatggg
     4681 agacgtttta gattatttag ttcagcgttt gacttagtta gaataataaa aagagcgtaa
     4741 gctcttttta ttattctt
//
LOCUS       DATXIG010000083         4729 bp    DNA     linear   BCT 28-DEC-2023
DEFINITION  TPA_asm: Streptococcus pyogenes strain MGAS11113
            SAMN08555799-rid19682583.denovo.083, whole genome shotgun sequence.
ACCESSION   DATXIG010000083 DATXIG010000000
VERSION     DATXIG010000083.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN08555799
            Sequence Read Archive: SRR6740495
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pyogenes
  ORGANISM  Streptococcus pyogenes
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 4729)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4729)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-OCT-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-OCT-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001918058.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001918058.2
            Genome Coverage       :: 368x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/12/2023 11:23:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 1,806
            CDSs (total)                      :: 1,769
            Genes (coding)                    :: 1,722
            CDSs (with protein)               :: 1,722
            Genes (RNA)                       :: 37
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1, 1 (5S, 23S)
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 47
            CDSs (without protein)            :: 47
            Pseudo Genes (ambiguous residues) :: 0 of 47
            Pseudo Genes (frameshifted)       :: 30 of 47
            Pseudo Genes (incomplete)         :: 20 of 47
            Pseudo Genes (internal stop)      :: 9 of 47
            Pseudo Genes (multiple problems)  :: 11 of 47
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4729
                     /organism="Streptococcus pyogenes"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN08555799-rid19682583.denovo.083"
                     /strain="MGAS11113"
                     /serotype="Emm28"
                     /isolation_source="normally sterile site"
                     /host="Homo sapiens"
                     /db_xref="taxon:1314"
                     /country="Finland"
                     /collection_date="2002"
                     /collected_by="National Public Health Laboratory"
     gene            complement(13..1281)
                     /locus_tag="VC122_001587"
     CDS             complement(13..1281)
                     /locus_tag="VC122_001587"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002989729.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PLP-dependent aminotransferase family protein"
                     /protein_id="HEP5641499.1"
                     /translation="MTTKYQTIISNIEQDIQKQRLKKGDKLPSIRVLSKVYHCSKDTV
                     QRALLELKYRHLIYAVPKSGYYVLGNVSMPDNVLNLSLEDYNNMAYEDFRLCLNEALS
                     AKDKYLFHYYHKTEGLEELREALLLYLAENSVYSNKDQLLITSGTQQALYILSQMPFP
                     NAGKTILLEKPTYHRMEAIVAQLGLPYQTISRHFNGLDLELLESLFQTGDIKFFYTIS
                     RFSHPLGLSYNTKEKEAIVRLAQRYQVYILEDDYLGDFVKLKEPPIHYYDTHHRIIYL
                     KSFSMSVFPALRIGALVLPSGLKPYFLTQKSLIDLDTNLLMQKALALYLENGMFQKNL
                     RFIKRYLKQRERQLALFLKQNCPDIHYQLTPTHLVIDYTTSDSYRNFTLDKSDRIIIT
                     GKKRYLSIAINQQIQSKLNSLIKNTCGKSN"
     gene            1371..2237
                     /locus_tag="VC122_001588"
     CDS             1371..2237
                     /locus_tag="VC122_001588"
                     /EC_number="2.7.1.35"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011888853.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxamine kinase"
                     /protein_id="HEP5641500.1"
                     /translation="MKRIIVANDLVGVGKVALSASIPLMASCLLEQVLLPTCLLSSHT
                     GGGQSPVKHTTSSILDVFLTDWDNKRIKFDGIFIGYFTTTRDGQKLLEWLSRKQLPLI
                     LDPIMADNGKLYQGFSQKHIEIMIKLTQKAELVLPNISEACLLTQTAYLAKNYDEEDI
                     RQVARKLAKLGPKQVVISGISFEEGSIGFAYYQSDSDQLTFHFGKQFPYHFYGTGDIA
                     SAIITAGFYYQLPLTQILDLVVSFLEKVLQNTLTLKRDLILGVAYESHLNYLTKSFKK
                     LLEEKNVISKIS"
     gene            2215..2772
                     /locus_tag="VC122_001589"
     CDS             2215..2772
                     /locus_tag="VC122_001589"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012560733.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ECF transporter S component"
                     /protein_id="HEP5641501.1"
                     /translation="MSSQKLANYIELSMYAALIFISVQVLRIPIGMQFIHLGNALVVI
                     AVLIYGSKKGALVASLGLGIFDVLNGYASVVWITILESLAICLILHLVYERAMQSSKK
                     PKAIITITVIAALSKLTINLIKYLLLAYIGTQLPLSVSFWVALGKIGGSFGTSFVTLV
                     AVPLLYPVIKNLKEKVTIGQAKRGF"
     gene            2906..4450
                     /locus_tag="VC122_001590"
     CDS             2906..4450
                     /locus_tag="VC122_001590"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003048363.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC-F family ATP-binding cassette
                     domain-containing protein"
                     /protein_id="HEP5641502.1"
                     /translation="MSILEVKQLSHGFGDRAIFENVSFRLLKGEHIGLVGANGEGKST
                     FMSIVTGHLQPDEGKVEWSKYVTAGYLDQHTVLESGQTVRDVLRTAFDELFKTENRIN
                     EIYASMADDKADIAVLMEEVGELQDRLESRDFYTLDAKIDEVARALGVMDFGMESDVT
                     SLSGGQRTKVLLAKLLLEKPDILLLDEPTNHLDAEHIEWLKRYLQHYENAFVLISHDI
                     SFLNDVINIVYHVENQSLVRYTGDYYQFQAVYEMKQSQLEAAYERQQKEIANLQDFVN
                     RNKARVATRNMAMSRQKKLDKMDIIELQAEKPKPNFEFKQARTPSRFIFQTKNLVIGY
                     DYPLTKEPLNITFERNQKIAIVGANGIGKSTLLKSLLGVIEPLEGHIVTGDFLEVGYF
                     EQEVTGVNRQTPLEVVWDAFPALNQAEVRAALARCGLTSKHIESQIQVLSGGEQAKVR
                     FCLLMNRENNVLILDEPTNHLDIDAKNELKRALKAYKGSILMVCHEPDFYNGWVTDTW
                     DFSKLT"
BASE COUNT         1531 a          701 c          910 g         1587 t
ORIGIN      
        1 tacgaagaaa agttagttgc tctttccaca agtatttttg attagcgagt ttaatttgct
       61 ttgtatttgt tgattgatag caatagagag atagcgcttt tttccagtta ttattattct
      121 atcagattta tctaatgtaa agtttctata agagtctgat gtcgtgtaat caatcactaa
      181 atgagtaggg gttagttgat agtgaatatc agggcaattt tgttttaaaa agagagccaa
      241 ttgacgttcc cgctgtttta gataacgctt aataaagcga agattttttt gaaacatccc
      301 attttctaaa taaagagcta atgctttttg cattagtaag ttcgtgtcta aatcaatcag
      361 tgatttttgg gttaaaaaat aaggttttaa acctgacggt aaaactagag ctccgatacg
      421 gagtgcagga aaaacactca ttgaaaaaga ttttaggtag atgattcggt gatgagtgtc
      481 ataataatga attggtggct ctttaagttt cacaaaatct cctaaataat catcttctaa
      541 aatataaacc tggtatcgtt gtgctagacg aacaatagct tccttttcct tagtgttata
      601 agaaagtccc agaggatgtg aaaatcgtga aatcgtgtaa aaaaatttaa tatcgccagt
      661 ttgaaataaa gattctaaga gttctaaatc aaggccatta aagtgtctag agatagtttg
      721 atagggtaat cctaactgag caacaatagc ttccatccga tgataagtag gcttttcaag
      781 gagaattgtt ttgcccgcat taggaaaagg catttgtgat aaaatataaa gcgcttgttg
      841 cgtcccagag gtgattaata gttggtcttt gttactgtaa acactatttt cagctaagta
      901 aagaagcaaa gcttccctta gttcttctag tccttcggtt ttgtgataat aatgaaagag
      961 gtatttgtct ttagcactta aagcctcatt taagcagagt cggaaatctt cataagccat
     1021 attattataa tcttcaagac ttagattaag aacattatcg ggcatgctta cgttacccaa
     1081 aacataataa ccacttttag gaacagcata aattaagtga cgatatttta attctaaaag
     1141 agcccgctga acagtatctt tgctacaatg ataaacctta cttaataccc taattgaagg
     1201 tagcttgtcc ccttttttta gtcgctgttt ctggatatct tgttcgatat tggaaatgat
     1261 tgtttgatat tttgttgtca taaattgtcc ccatacagtt aagtgttata actattgtat
     1321 cttatgcctc tttttcttac aatagttatt atagaaaaga ggtaatagct atgaaacgga
     1381 ttattgtggc aaatgatctg gttggtgttg gtaaggtagc tttgtctgct agtattcctt
     1441 taatggcttc ttgtttgtta gagcaagttt tattaccaac ttgcctttta tcaagccata
     1501 ctggcggagg acagagccct gtgaagcata ctacatcaag tatattagac gtttttttaa
     1561 cagattggga caacaaaaga attaagtttg acggcatctt tataggttat tttacgacaa
     1621 caagagatgg gcaaaagcta ctagaatggc tttcaagaaa gcaacttccc ttaattttag
     1681 atccaattat ggcagataat ggaaaacttt atcaaggctt cagtcaaaaa catatagaga
     1741 ttatgataaa acttactcaa aaagctgagc ttgttttacc taatattagt gaggcttgtc
     1801 tattaacaca aacagcttat ttagcaaaaa attatgatga agaagatatt agacaggtgg
     1861 ctagaaaatt agctaagctt ggacctaaac aggttgtcat tagcggaatc tcttttgagg
     1921 agggcagcat tggttttgct tactatcagt cagattcaga tcaattaaca ttccattttg
     1981 ggaagcaatt cccttaccat ttctatggta caggagatat agcatcagct attataacag
     2041 caggattcta ttatcagcta ccactaacac aaattctaga tcttgttgtt tcatttcttg
     2101 aaaaagtatt gcaaaatacc cttacattaa aaagagattt aatcttaggt gttgcttatg
     2161 aatctcattt aaattactta actaaatcgt ttaaaaagtt gttggaggaa aaaaatgtca
     2221 tctcaaaaat tagctaatta tattgaattg agtatgtatg ccgctttaat ttttatttca
     2281 gtacaagtgt tgcgtatccc tatcggtatg cagtttattc atcttggcaa tgcattagtt
     2341 gttattgcag tcttgatcta tggtagtaaa aaaggagctc tagttgctag tcttggttta
     2401 gggatctttg atgttttgaa cggctatgct tcagtcgttt ggataactat cttagaatcc
     2461 ttggctattt gtttaatttt gcatttggta tatgaaagag ctatgcaatc tagtaaaaaa
     2521 ccaaaagcca ttataacaat aacagttatt gctgcattgt caaaattaac tattaacctt
     2581 ataaaatact tactgttagc ttatataggt actcagctac cactttcggt atctttttgg
     2641 gtagctcttg gaaaaattgg tggtagtttc ggaacatctt ttgtgacttt agttgctgtt
     2701 cctcttttgt atcctgttat caaaaactta aaagaaaagg ttacaatcgg acaagcaaaa
     2761 agaggttttt gatacaagta ccttatcgat aaaaaagcaa ggtacacttt cagtcaaata
     2821 ttgacaaaag gttacaattt tgccataatg aaataactat aaagccgttt tgacggcttt
     2881 tttgatgaca gttaaggaag aaacgatgag tattttagaa gttaagcagt tgagtcacgg
     2941 ttttggggat cgggctattt ttgaaaatgt gtcatttcgc ctcttaaaag gtgaacatat
     3001 tggactagtt ggagcaaatg gtgaaggaaa atcaaccttt atgagtatag tcacaggaca
     3061 tttacagcct gatgaaggaa aagtagagtg gtcgaagtat gtcactgcag gttacctgga
     3121 tcaacataca gtgttggaat caggacaaac cgttcgtgat gtcttgcgaa ctgcttttga
     3181 tgagttattt aagaccgaga atcgtattaa tgagatttac gcgtcaatgg cagatgataa
     3241 agctgatatt gctgttttga tggaagaagt aggtgagctt caagatcgtt tagaaagtcg
     3301 tgatttctat actttggatg ctaagattga tgaagtagcg cgtgcgcttg gtgttatgga
     3361 ttttggaatg gagtcagatg ttacatcctt atcaggtggg caacgaacaa aggttttatt
     3421 agccaaatta ctattagaaa aacctgatat tctgctatta gatgaaccaa ctaaccattt
     3481 ggatgctgag catattgaat ggttaaaacg ctatttacaa cattatgaaa atgcttttgt
     3541 gttgatttcg catgatattt ctttcttaaa tgatgtgatt aatattgttt accatgttga
     3601 aaatcaaagt ttagttcgct atactgggga ttattaccaa tttcaagctg tttatgagat
     3661 gaaacaatct caacttgaag cagcctatga acgtcaacaa aaagagattg ctaacttgca
     3721 ggattttgtc aaccgaaata aggctcgggt agcgacacgt aacatggcaa tgtctcgcca
     3781 aaaaaaactt gataagatgg atataattga acttcaagct gagaaaccaa aaccaaattt
     3841 tgaatttaag caagctagaa ctcccagtcg attcattttt caaacaaaaa atcttgtgat
     3901 tggttatgat tacccattga ccaaagaacc cttaaatata acgtttgaaa gaaaccaaaa
     3961 aattgctatt gttggggcca acggtattgg aaaatctact ttgctaaaaa gtttattagg
     4021 tgttatcgag cctttagaag gtcatattgt cacaggggat tttttagaag ttggctactt
     4081 tgaacaagaa gtgacaggtg ttaaccgaca aactccgcta gaagtagttt gggatgcttt
     4141 tcctgcctta aatcaggcag aagttcgagc ggcactagct cgttgcggac taacatcaaa
     4201 acatattgaa agtcaaattc aagtactttc gggtggtgaa caagcaaaag ttcgtttttg
     4261 tttgttgatg aatcgtgaaa ataacgtgct tattttagac gaaccaacaa atcatcttga
     4321 tattgatgct aaaaatgagc tcaaacgtgc tttaaaagca tataagggtt ctattttaat
     4381 ggtttgtcat gaacctgatt tctacaatgg gtgggtaacc gatacttggg attttagtaa
     4441 gttaacctaa actattcctt aattttttac acaggaatta ctaaaatcag taaagatata
     4501 gaataaatac tgctagatat gtgatggttt aggtaactga tcaccaacca aaaaagatgt
     4561 gactacatct tttttttatc taatggaatg gtgtaaagtc gtttaataat agcttatttt
     4621 ttgatgatca gttgttcatg aaattgttgg tgattaacaa gtcatttaag atacaaacgt
     4681 tattactaaa aagtgattca aatagtttcc ctattttttt atgtttttt
//
LOCUS       DATXIG010000084         4534 bp    DNA     linear   BCT 28-DEC-2023
DEFINITION  TPA_asm: Streptococcus pyogenes strain MGAS11113
            SAMN08555799-rid19682583.denovo.084, whole genome shotgun sequence.
ACCESSION   DATXIG010000084 DATXIG010000000
VERSION     DATXIG010000084.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN08555799
            Sequence Read Archive: SRR6740495
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pyogenes
  ORGANISM  Streptococcus pyogenes
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 4534)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4534)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-OCT-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-OCT-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001918058.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001918058.2
            Genome Coverage       :: 368x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/12/2023 11:23:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 1,806
            CDSs (total)                      :: 1,769
            Genes (coding)                    :: 1,722
            CDSs (with protein)               :: 1,722
            Genes (RNA)                       :: 37
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1, 1 (5S, 23S)
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 47
            CDSs (without protein)            :: 47
            Pseudo Genes (ambiguous residues) :: 0 of 47
            Pseudo Genes (frameshifted)       :: 30 of 47
            Pseudo Genes (incomplete)         :: 20 of 47
            Pseudo Genes (internal stop)      :: 9 of 47
            Pseudo Genes (multiple problems)  :: 11 of 47
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4534
                     /organism="Streptococcus pyogenes"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN08555799-rid19682583.denovo.084"
                     /strain="MGAS11113"
                     /serotype="Emm28"
                     /isolation_source="normally sterile site"
                     /host="Homo sapiens"
                     /db_xref="taxon:1314"
                     /country="Finland"
                     /collection_date="2002"
                     /collected_by="National Public Health Laboratory"
     gene            280..780
                     /locus_tag="VC122_001591"
     CDS             280..780
                     /locus_tag="VC122_001591"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011284552.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEP5641503.1"
                     /translation="MKKTLTLLLALFTIGVTSSVRAEESSWVPLPRQQKVLDDNRNEI
                     PVHITGNKVTVTLPPGWSAWIDKNTKQRNSNSVTTTVTLDISTSQPPKEFTIYFQGDD
                     GFLAGMMTFIDSETLKNEQYKAFDEEYAENLFENSRLREEQKTWRDKVRDVFQDAWWN
                     FKGLFQ"
     gene            complement(1153..1404)
                     /locus_tag="VC122_001592"
     CDS             complement(1153..1404)
                     /locus_tag="VC122_001592"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014407356.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEP5641504.1"
                     /translation="MPEKKVLPVLSMVVSFVCLLDHIFFFATYSYLYLPLVASSLALI
                     SLVQNKGRQKVWSLAGLILAALAVAVVAYAYYDPTVIPY"
     gene            complement(1657..2130)
                     /locus_tag="VC122_001593"
     CDS             complement(1657..2130)
                     /locus_tag="VC122_001593"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011017434.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEP5641505.1"
                     /translation="MKTKSKRFLNLATLCLALLGTTLLMGQPIKAEAVVSDLNDAVSS
                     MGDTAEETDVNIDGQEDEEDEEDDAYMQGRRLGEKEGYEAGQKDNAPRLFDSSTLEPP
                     TNPYEDAGDTLNSNWFKSGYSDAYGQAYEQGWNDTHPIQSTLIWLWGIVRNLFRF"
     gene            2586..3838
                     /locus_tag="VC122_001594"
                     /pseudo
     CDS             2586..3838
                     /locus_tag="VC122_001594"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014293804.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ISL3 family transposase"
     gene            complement(4145..4501)
                     /locus_tag="VC122_001595"
     CDS             complement(4145..4501)
                     /locus_tag="VC122_001595"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEP5641506.1"
                     /translation="MSEIGHDHVTISSNGQTDEGAAYGRGHDDGSKFGYEAGLQSSWN
                     ESEPPSSDKIPEPSVNPYESSNEQDREDYKEGFRDGYPGGYVAGWRKTHPIEATLQYL
                     WYTVSSWFESLFNNSK"
BASE COUNT         1468 a          996 c          790 g         1280 t
ORIGIN      
        1 tggactaaat aagataagag ctttcaataa atatgagcaa ttgccctata agatattgat
       61 accaaatata aaaccgctca taaaatatga cataataccc aaacgacctt taaaggtaaa
      121 atggcaacag ctagcgactg actaggaaaa aaactagctg atcctaccaa gaatagtcgc
      181 gccatttccc aaccacaaga catgaactcc ctatcttttt agcgcaagaa ttggtataat
      241 aacccttgtc ttaaaaaatg agcaaaagga gaggaaaaaa tgaaaaagac attaactttg
      301 ctactggcac tctttaccat cggggtaact agtagcgtca gagcggagga gagttcttgg
      361 gtacctcttc caagacaaca aaaagtttta gatgataaca gaaatgaaat tcctgttcac
      421 attacaggta ataaagtcac tgttacactt cctccaggat ggtctgcatg gattgataaa
      481 aacacgaaac aacgaaacag caattcagtg actactacag tcactctcga tatctcaacg
      541 tcgcaacctc caaaagaatt tactatttat tttcaaggag atgatggatt tttagctgga
      601 atgatgactt ttattgattc agaaacactc aaaaatgaac aatataaagc atttgatgaa
      661 gaatatgccg aaaacctttt tgaaaattct cgtctaagag aggaacaaaa aacatggcgg
      721 gataaagtac gcgatgtttt tcaagacgct tggtggaact ttaagggact gtttcagtga
      781 gggtttgcta ggctatgagg aggttggcta aagtacaaaa agcaccctta caggtgctta
      841 gaacctagac aaacctagtt ctatgttcaa aaagcataaa cagctctgct gatgtgacat
      901 acggtttccg atttttacac acagagggag ccaaaacaga tgatttttgt catggtgaaa
      961 gtggcgtcaa tacgagagga cgccactcgg gatttttgag accttaggtc tcaaaaataa
     1021 gtcagggaat tccaaaggaa gtcgctaccg tccgcaccgc ctcagaaaaa gtatcacaaa
     1081 agaccttttt caatctaagc acctgtaagg gtgctttttg tgctgctgat tgcgtgcaaa
     1141 gaggagataa ggttaatagg ggatgacggt tgggtcatag taggcatagg ccactactgc
     1201 cacggctaga gcagctaaga taagcccagc tagcgaccaa accttttgcc tgcctttgtt
     1261 ttgcaccaga ctgataagag ctagactact tgctacaagc ggcaagtaga gataactata
     1321 agtagcaaaa aagaagatat gatcaagcag gcagacaaaa ctcaccacca tgcttaagac
     1381 gggcaacact ttcttttctg gcataaatgc tcctttctca acacacgggt cactaaaacc
     1441 caaaaaagcc acccctatca gaggtggcct gactactaaa gggactatcc caattatagc
     1501 agattagtgt gggaaatgct tgtgataaaa ggattcattt tagtgttata gtatttcaaa
     1561 tatctcaaaa gagtaaataa cactatcctc aaaagcgata ctagcttttt ataacgtaat
     1621 attaattact ttgttgttgg taaccgtcac ctaaatctaa aatctaaata agttccttac
     1681 tatcccccac agccaaatta aagttgattg tatcggatga gtatcattcc atccctgctc
     1741 atatgcttga ccataagcat cgctgtaacc acttttaaac caattactat tcaatgtatc
     1801 acccgcatcc tcgtaaggat tagtaggggg ctctagcgta ctactgtcaa ataatctggg
     1861 agcgttgtct ttttgaccag cttcataccc ctctttctct cccagtcttc tcccttgcat
     1921 ataagcatcg tcttcttcgt cttcttcgtc ttcttgacca tcgatattga cgtcagtttc
     1981 ctctgctgta tctcccatcg aagagactgc atcattcaaa tcagatacta ctgcctccgc
     2041 ctttatgggt tgtcccatta gcaaagtcgt tcctagtagg gccaagcaaa gggttgctag
     2101 atttaaaaag cgttttgatt ttgttttcat aaaatcactc catttcatta tttttgatac
     2161 ggtcatagtt tactactcgc actctttcat ttcaagttca tttttttatc attttaaaca
     2221 tcaaatgatt agaaaatcat ggcaatctct tattacctca gaaagtgctt gcttggggct
     2281 gttagtccgc ctttttgcgc ttaggagttt ttttgaaagc ctttgatgtt ttgtgttaga
     2341 ccttactagc aactacagtg aaagcgtgga catttgcgtc tattttaata gggaatagct
     2401 gtcggttcat attatactgt tactgctact acaagcccag ccagcgaaca cttgcttttg
     2461 ttttatacaa gacctacaat gctagactac ttgctacaaa cggcaagtag aggtaactat
     2521 aagtagcaaa aaatcccata tgacttataa tgcaaagcga ccaaaccatc attagaaaga
     2581 atcatatgag acaactctat catactacag aattgattag aattaaagat aaacacatca
     2641 ccttgaacaa ggtttttcaa cgcgagacac ttatcgaagt cttcgccaca cttgactatc
     2701 cctacctaag tgtaaacact gtaaaggagt tcagattaaa tacgactccc agaaacattc
     2761 taaaatcccc tttatcgaaa ttggtggttt tcctagtctc attcgtttaa aaaagagaag
     2821 atctcaatgc aaatcctgtc gaaaagtggc tgtagcggaa acaagtctgg ttcaaaaaaa
     2881 ctgtcagatc tctgagcccc tcagacaaaa agtcgcacaa accttggtta atcgaggagc
     2941 tctcactaac atcgctcaac agtcggctat atcatcttct accgtctacc gaaaactcaa
     3001 ccagtttgta atcaaggaag acttctctaa actacccgag gtgttgtctt gggatggatt
     3061 ttcctatcaa aagggcgaac tcgcctttat cgctcaagat tttgagtcca aaaaaatcat
     3121 ctccatactg gacaaccggc gacaaacaac cattcgaaat cacttcttta gatactctaa
     3181 agaggctaga aacagtgtca aaatcgttac cgttgatatg tcaggaagct atatttcgct
     3241 cattaaaaag ctatttcctc aagctaacat cgtcctagat cgtttcctat tgttcagcac
     3301 atgaacagag cgctcagtca aacccggatt cacatcatga agcaatatga caaaaagtct
     3361 ctggaataca gagttcttaa atactactgt caactcattc aaaaggtagc cgaaaactct
     3421 cgctcaacgc cttctactct agaaccttta gggaaacgct aactcctaaa caatgtctca
     3481 ataaactatt taatcccgtt cctgaattaa aagattacta tgacctttat cagttactcc
     3541 tttttcactt acaagagaag aatactgagc attttttgac ttaattcaaa aggtgttact
     3601 tctgctcaat aacatcttta aaacggcttt gaacaccctg atgcgataca aaaactatgt
     3661 cactaatgtc attgagcttc cttgttcaaa cgccaaacta gaagcaacca ataaactcat
     3721 caaacacatc aaacgcaatg cttttggctt tcggaacttt gataacttta aaaaacgcat
     3781 ttacctcgct ttgaacataa caaaagagaa gacgagtctc gtctcctcac gtgtttagct
     3841 ataagtcacc cactacagtt gacaaagagc ccaaaaaaga agatatgatc aagcaggcag
     3901 acaaaactca ccaccatgct taggacgggc aacactttct tttctggcat aaatgctcct
     3961 ttttcaacac acgggtcact aaaacccaaa aaaggccacc cctatcagag gtggcctgac
     4021 tactaaaggg attatcccaa ttatagcaga ttagtgtggg aaaggtaaaa caaaagccac
     4081 tagcacgaag ttgctgacag acattttatt ttaccttaag taactttaat caacaaacaa
     4141 atatctactt actattatta aataagcttt caaaccaact agataccgta taccataaat
     4201 attgaagagt agcttctatt ggatgagttt ttctccagcc tgcgacatag cccccaggat
     4261 atccatcacg aaaaccttct ttataatcct ctctatcttg ttcattacta ctttcatatg
     4321 gattcacact aggctcaggt attttatctg atgatggggg ttcagactca ttccaactcg
     4381 attgtaatcc tgcttcatat ccaaacttac ttccatcatc atggcctcta ccatatgcag
     4441 caccctcatc tgtttgacca ttagaactga ttgttacgtg atcgtgccca atttctgaca
     4501 cctccgcctt tacgggacgt gtcattagca aagt
//
LOCUS       DATXIG010000085         4469 bp    DNA     linear   BCT 28-DEC-2023
DEFINITION  TPA_asm: Streptococcus pyogenes strain MGAS11113
            SAMN08555799-rid19682583.denovo.085, whole genome shotgun sequence.
ACCESSION   DATXIG010000085 DATXIG010000000
VERSION     DATXIG010000085.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN08555799
            Sequence Read Archive: SRR6740495
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pyogenes
  ORGANISM  Streptococcus pyogenes
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 4469)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4469)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-OCT-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-OCT-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001918058.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001918058.2
            Genome Coverage       :: 368x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/12/2023 11:23:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 1,806
            CDSs (total)                      :: 1,769
            Genes (coding)                    :: 1,722
            CDSs (with protein)               :: 1,722
            Genes (RNA)                       :: 37
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1, 1 (5S, 23S)
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 47
            CDSs (without protein)            :: 47
            Pseudo Genes (ambiguous residues) :: 0 of 47
            Pseudo Genes (frameshifted)       :: 30 of 47
            Pseudo Genes (incomplete)         :: 20 of 47
            Pseudo Genes (internal stop)      :: 9 of 47
            Pseudo Genes (multiple problems)  :: 11 of 47
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4469
                     /organism="Streptococcus pyogenes"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN08555799-rid19682583.denovo.085"
                     /strain="MGAS11113"
                     /serotype="Emm28"
                     /isolation_source="normally sterile site"
                     /host="Homo sapiens"
                     /db_xref="taxon:1314"
                     /country="Finland"
                     /collection_date="2002"
                     /collected_by="National Public Health Laboratory"
     gene            30..875
                     /locus_tag="VC122_001596"
     CDS             30..875
                     /locus_tag="VC122_001596"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002982833.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mechanosensitive ion channel family protein"
                     /protein_id="HEP5641507.1"
                     /translation="MTFILHYLEQSHIENIGLTIFKKLVSLIILLLFFAILKRVTNYL
                     FEKTINKSFAYSRQSEARKKTLSKLTHNILNYLLYFLLIYWILSLFGIPVSSLLAGAG
                     IAGVAIGLGAQGFLSDVVNGFFILFENQFEVGDNVTISDIEGSVFGVGIRTTQIRGFD
                     GTLHFIPNRSITVVSNKSRGNMRALIEIPLYSTVNLSQVTRIIDEVNQKELPNHPQIV
                     GKPNILGPQNNSNGQFTFRIAIFTENGEQFKIYHTFYRLYQEALLKEGIQLPTAISYS
                     ITSKS"
     gene            complement(940..1500)
                     /locus_tag="VC122_001597"
     CDS             complement(940..1500)
                     /locus_tag="VC122_001597"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009880946.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR01440 family protein"
                     /protein_id="HEP5641508.1"
                     /translation="MLNNLEKQTREIVIDVVERSAIQPGNLFVLGLSSSEILGSRIGK
                     QSSLEVGQIVVEVVLDELNKRGVHLAVQGCEHVNRALVVERHVAESKQLEIVNVVPNL
                     HAGGSAQMAAFQFMSDPVEVEEVIAHAGLDIGDTAIGMHIKRVQIPLIPCQRELGGAH
                     VTALASRPKLIGGARADYNMDIIRKS"
     gene            complement(1514..1984)
                     /locus_tag="VC122_001598"
     CDS             complement(1514..1984)
                     /locus_tag="VC122_001598"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002982824.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ECF transporter S component"
                     /protein_id="HEP5641509.1"
                     /translation="MQNSKIRQMSLTGILTALVVVLGRFVMLPTPTGFLTLLDAGIYA
                     VSFSFGSAQGAIVGGLSGFLIDLVAGYPQWMFHSLIAHSVQGYFAGWRGRKRWLGVVI
                     GSFIMIFWYFLGSLMLGYGLSGSLAGIWGNVMQNTLGLFVGFIIFKAILRQKKR"
     gene            complement(1974..2738)
                     /locus_tag="VC122_001599"
     CDS             complement(1974..2738)
                     /locus_tag="VC122_001599"
                     /EC_number="2.7.4.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009880948.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional hydroxymethylpyrimidine
                     kinase/phosphomethylpyrimidine kinase"
                     /protein_id="HEP5641510.1"
                     /translation="MKTDYIVTISGNDILSGGGLYADLATYIRYDLQAFVAVTCLTTR
                     SEEGFSLFPVAKEIFRDQLNSFTNAPISAIKIGLLPNAEMCEIVLDFIKGHLGIPVVL
                     DPVLACKEIDDVKIVPLRQEILQLLPYVTVVTPNLVEAQLLSQKEIVSLKDMQEAAKY
                     FYQLGAKQVVIKGGNRFSQKKAIDLFYDGKEIVTLECPVLEKNNIGAGCTFASSIASQ
                     LVKKKTPLEAVKNSKELVYQAILQSDRYGVKQSYAK"
     gene            complement(2728..3477)
                     /gene="truA"
                     /locus_tag="VC122_001600"
     CDS             complement(2728..3477)
                     /gene="truA"
                     /locus_tag="VC122_001600"
                     /EC_number="5.4.99.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002988132.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA pseudouridine(38-40) synthase TruA"
                     /protein_id="HEP5641511.1"
                     /translation="MVRYKATISYDGTLFSGFQRQRHLRTVQEEIEKTLYKLNNGTKI
                     IIHGAGRTDAGVHAYGQVIHFDLPQEQEVEKLRFALDTQTPEDIDVVNIEKVADDFHC
                     RYQKHLKTYEFLVDNGRPKNPMMRHYTTHYPYTLNIKLMQEAINGLVGTHDFTGFTAA
                     GTSVQNKVRTITKATVSRDEKTDFLVFTFSGNGFLYKQVRNMVGTLLKIGNGQMPVEQ
                     VKVILSSKNRQLAGPTISGNGLYLKEICYEN"
     gene            complement(3662..4243)
                     /locus_tag="VC122_001601"
     CDS             complement(3662..4243)
                     /locus_tag="VC122_001601"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011888599.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HEP5641512.1"
                     /translation="MHNEKSNRTLVSCCFFVVLFILGIVKKKRRLKMSIETRAAFEKV
                     KPIILKLKRHYYIQLWDRDDWLQEGHIILLQLLERYPELIEEEERLYRYFKTKFSSYL
                     KDLLCRQESQKRQFHKLAYEEIGEVAHAIPSRGLWLDDYVAYQEVIASLENQLNSQER
                     MQFQALIRGERFKGRRALLRKISPYFKEFAQQL"
     gene            complement(4239..4312)
                     /locus_tag="VC122_001602"
     tRNA            complement(4239..4312)
                     /locus_tag="VC122_001602"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(4276..4278),aa:Arg,seq:acg)
     gene            complement(4392..4465)
                     /locus_tag="VC122_001603"
     tRNA            complement(4392..4465)
                     /locus_tag="VC122_001603"
                     /product="tRNA-Asn"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(4429..4431),aa:Asn,seq:gtt)
BASE COUNT         1416 a          989 c          659 g         1405 t
ORIGIN      
        1 ttgataaact atcaaagaaa ggatttctca tgacatttat attacattac ctagagcagt
       61 ctcatatcga aaatattggc ttaacgattt ttaaaaagct agtgtctctc attattctat
      121 tactcttttt tgctatttta aaacgtgtta ctaactatct ttttgaaaaa accattaaca
      181 aatcgtttgc ttattcaaga caaagtgaag ctcgcaaaaa aacactctct aagctcactc
      241 acaatatttt aaattactta ttgtattttc ttctcattta ttggatactt agcttatttg
      301 gcatacctgt ctctagtctt ctagcaggag ctggaattgc tggagtagca atcgggttag
      361 gtgcacaggg atttttatct gacgttgtca atggcttttt tatcttattt gagaatcaat
      421 ttgaagtagg agataacgtt actatctctg acatagaagg cagtgtcttt ggtgttggaa
      481 ttagaactac tcaaattcgt ggattcgatg gaacactaca tttcatccca aaccgtagca
      541 tcaccgttgt cagcaacaaa tctcgtggta atatgcgtgc cttaattgaa attcctcttt
      601 attccactgt taatttgagc caagtcactc gaatcattga tgaagtgaat caaaaggaat
      661 tgccaaacca tcctcaaatc gttggcaaac caaatatttt agggcctcaa aataactcta
      721 atggacagtt cacttttaga attgctattt tcacagaaaa tggagaacaa tttaagattt
      781 accatacttt ttatagactt tatcaagaag ctttactcaa agaaggcatt caattaccga
      841 ctgcaatcag ctattctatc acctcaaaat cttaatataa tcttaaaaaa tccttttttc
      901 tagaaaaaac accaattgtt ttattccaga aaaaagggtt taggatttac ggataatatc
      961 catattgtaa tcagccctag ctccaccaat taattttggt cgactagcca aagctgtcac
     1021 atgagcacct cctagctctc gttgacaagg aatcagaggg atctgaacac gcttaatatg
     1081 cattcctata gcagtatcac ctatatcaag tccagcgtgt gcaataactt cttctacttc
     1141 gacagggtct gacataaatt gaaaagcagc catctgagca ctgccaccag catgaagatt
     1201 cggaaccaca ttgacaatct ctaactgctt agattctgcc acatgcctct ctactaccaa
     1261 ggcacgattg acgtgttcac aaccttgtac agccaaatga acaccacgct tatttaattc
     1321 atctaacaca acttcaacaa ctatttgacc tacttctaga ctagattgtt ttccaattct
     1381 actacccaaa atttcgctag atgaaagacc tagaacaaaa agattacctg gttgaatagc
     1441 agagcgttct acaacatcaa tgacgatttc ccttgtttgt ttctctaaat tattcaacaa
     1501 cttctcactc tccttatctc tttttttgcc ttaatatggc cttaaagata ataaaaccaa
     1561 caaaaagccc taatgtattt tgcatgacgt ttccccaaat accggctaaa gatcccgaaa
     1621 ggccataacc aagcataaga gagcctaaaa agtaccaaaa aatcatgata aaagaaccta
     1681 tcacaacacc aagccaacgc ttgcgccctc tccatcccgc aaaataccct tggacactgt
     1741 gagcaatcaa actatgaaac atccactgag ggtatcctgc cactaaatcg attaaaaaac
     1801 ctgataaccc tcccacaata gctccttgag ctgatccaaa ggaaaagctg acagcataaa
     1861 ttcctgcatc caacagcgtt aaaaaacctg ttggagtagg tagcatgaca aatcttccta
     1921 aaacaacaac caatgctgtc aatatccctg ttaaactcat ttgtcgtatc ttactatttt
     1981 gcataacttt gttttactcc gtatctatca gattgtaaaa tagcttggta gactaactct
     2041 tttgagtttt tcacagcttc tagtggcgtt tttttcttta ctaactgact agcgatacta
     2101 gatgcaaaag tacagcctgc gccaatatta tttttctcaa gtacaggaca ttccaaggta
     2161 acaatctctt taccgtcata aaaaagatca atagcctttt tctgactaaa tcgattacca
     2221 cccttgataa ccacctgttt tgccccaagt tgataaaaat atttagcagc ttcttgcata
     2281 tcttttaaag aaacaatctc tttttgagat aatagctgag cctctaccaa atttggagta
     2341 acaacagtta cataaggcaa taattgtaaa atctcctgtc ttagaggaac tattttaaca
     2401 tcatctattt ctttacatgc tagaacagga tctaaaacca ctgggattcc cagatgcccc
     2461 tttataaaat ccaaaactat ttcacacatt tccgcattag gtaacaaacc aattttaata
     2521 gcagaaattg gagcgttcgt aaagctattc aactgatcac ggaaaatttc tttagctacc
     2581 ggaaatagac taaagccctc ttcagatcta gtagttaaac aagttaccgc tacaaaggcc
     2641 tgtaggtcat accttatgta agtcgctaaa tcagcataga gaccacctcc acttaaaata
     2701 tcgttcccag atatagtcac gatataatca gttttcataa caaatctcct tcaagtaaag
     2761 cccatttcca gatatcgttg gtccagctag ctgacgattt ttggagctca aaatcacttt
     2821 aacttgttca actggcattt gaccattacc aattttcagc aaagttccca ccatattcct
     2881 cacctgctta tagagaaaac catttcctga aaaagtaaac accaaaaaat cagttttttc
     2941 atcccgagat accgttgctt tggtaatcgt tcgcacctta ttttgtaccg aagttcctgc
     3001 tgctgtaaaa ccagtaaaat cgtgtgtacc taccagaccg ttaatagctt cttgcataag
     3061 tttaatatta agagtataag ggtaatgagt ggtatagtgt cgcatcatcg gatttttagg
     3121 tcgaccatta tcaactaaaa attcataagt ttttaaatgc ttctgataac gacaatgaaa
     3181 atcatcagcc actttttcta tattaacaac atcaatatct tcaggagtct gtgtatcaag
     3241 agcaaatcgt aatttttcaa cttcttgttc ttgaggcaaa tcaaaatgga tcacctgtcc
     3301 ataagcatgt actcctgcat ccgtacgtcc tgcaccatga attataattt ttgtaccgtt
     3361 atttaattta tagagtgttt tttcaatctc ttcttgaacc gtacgtaaat gtctttgtct
     3421 ttgaaaaccc gaaaaaagag tcccatcata ggaaatcgtt gctttgtatc ttaccatgtt
     3481 actattttaa catttcttaa acgtaagaga aactattttt aatcaaactg tacccaaaca
     3541 aatgaaagac gtattgaagc ttactctaag ccactagcga tttaaaagct ataagaaagc
     3601 tatttcctcc aaactctctc cttttttcgt agatttaccc ctaattcctt ataggtcttg
     3661 cttacaactg ctgtgcaaat tccttaaaat agggactgat cttcctaagt agagcacgtc
     3721 ttcccttgaa acgttcgccc ctgataagtg cttgaaactg catacgctct tgcgaattta
     3781 attggttctc taagctagct attacctctt gataagccac atagtcgtct agccataacc
     3841 ctctcgatgg aatggcatgt gcaacctccc ctatctcttc atatgctaac ttatggaact
     3901 gacgcttttg actttcttga cggcataata aatctttcaa ataagatgaa aattttgttt
     3961 taaaatagcg atataaacgc tcttcttcct caattaactc tgggtacctt tctagtaact
     4021 gtaataagat aatatgtcct tcttgtaacc agtcatctct atcccacaat tgaatataat
     4081 agtgtcgctt taatttcaaa ataatgggct taactttttc aaacgctgct cttgtctcta
     4141 tcgacatttt gagtctcctt ttctttttaa ctatccctag tataaagaga accacaaaaa
     4201 aacaacagga cactaatgtc ctgttgcttt tttcattatg caccctagag gagtcgaacc
     4261 tctaaccgcc tgattcgtag tcaggtactc tatccagttg agctaagggt gccaacgcat
     4321 atcttgcctc tatcttacca cactcttctc taaaatgcaa aaaaagatag gacgcttcct
     4381 acctctctct actccgccag taggactcga acctacgaca tcatgattaa cagtcatgcg
     4441 ctactaccaa ctgagctatg gcggataaa
//
LOCUS       DATXIG010000086         4457 bp    DNA     linear   BCT 28-DEC-2023
DEFINITION  TPA_asm: Streptococcus pyogenes strain MGAS11113
            SAMN08555799-rid19682583.denovo.086, whole genome shotgun sequence.
ACCESSION   DATXIG010000086 DATXIG010000000
VERSION     DATXIG010000086.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN08555799
            Sequence Read Archive: SRR6740495
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pyogenes
  ORGANISM  Streptococcus pyogenes
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 4457)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4457)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-OCT-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-OCT-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001918058.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001918058.2
            Genome Coverage       :: 368x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/12/2023 11:23:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 1,806
            CDSs (total)                      :: 1,769
            Genes (coding)                    :: 1,722
            CDSs (with protein)               :: 1,722
            Genes (RNA)                       :: 37
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1, 1 (5S, 23S)
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 47
            CDSs (without protein)            :: 47
            Pseudo Genes (ambiguous residues) :: 0 of 47
            Pseudo Genes (frameshifted)       :: 30 of 47
            Pseudo Genes (incomplete)         :: 20 of 47
            Pseudo Genes (internal stop)      :: 9 of 47
            Pseudo Genes (multiple problems)  :: 11 of 47
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4457
                     /organism="Streptococcus pyogenes"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN08555799-rid19682583.denovo.086"
                     /strain="MGAS11113"
                     /serotype="Emm28"
                     /isolation_source="normally sterile site"
                     /host="Homo sapiens"
                     /db_xref="taxon:1314"
                     /country="Finland"
                     /collection_date="2002"
                     /collected_by="National Public Health Laboratory"
     gene            2..616
                     /locus_tag="VC122_001604"
     CDS             2..616
                     /locus_tag="VC122_001604"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000615743.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CadD family cadmium resistance transporter"
                     /protein_id="HEP5641513.1"
                     /translation="MIQNVVTSIILYSGTAVDLLIILMLFFAKRKSRKDIINIYLGQF
                     LGSVSLILLSLLFAFVLDYIPSKEILGLLGLIPIFLGLKVLLLGDSDGEAIAKEGLSK
                     DNKNLIFLVAMITFASCGADNIGVFVPYFTTLNLANLIVALLTFLVMIYLLVFSAQKL
                     AQVPSVGETLEKYSRWFIAVVYLGLGMYILIENNSFDMLWAVLG"
     gene            628..966
                     /gene="cadX"
                     /locus_tag="VC122_001605"
     CDS             628..966
                     /gene="cadX"
                     /locus_tag="VC122_001605"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000711077.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Cd(II)/Zn(II)-sensing metalloregulatory
                     transcriptional regulator CadX"
                     /protein_id="HEP5641514.1"
                     /translation="MKKDSICQVGVINQQNVTTATNYLEKEKVQKSLRILSKFTDNKQ
                     INIIFYLLAVEELCVCDIACLLNLSMASASHHLRKLANQNILDTRREGKIIYYFIKDE
                     EIRDFFNQLG"
     gene            1017..1940
                     /locus_tag="VC122_001606"
     CDS             1017..1940
                     /locus_tag="VC122_001606"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014635789.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HEP5641515.1"
                     /translation="MKFFEENYSQEIPTRIKNLRKKHNITQSELGNAGQVSQVESGKR
                     PITSSMLVYLNALTASSYTYIVFGELDEFIENLFHYFFSSILYRDLEAVDENLYSFMS
                     DDLISIQSSCLSIAKTFANFNIQRKRFMISTETEMDTFHKKDDIDVWVGGKSYNPARS
                     FRNNPINELTVIDFEEMADILLLMLRDNLIRSFEINVCNTLFELDKNGEPTTFNLDKI
                     DSIINKWWSENVSTEIIPNLIKKLRENPLFNIGFMVNDILERMYKEDIPKSYLTSVPL
                     VISKKARSTLSIKSNGSQKIDKSKFAQILSL"
     gene            2006..2758
                     /locus_tag="VC122_001607"
     CDS             2006..2758
                     /locus_tag="VC122_001607"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002991388.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA translocase FtsK"
                     /protein_id="HEP5641516.1"
                     /translation="MAIPDLDKFSSNLQQGKIPVIKHFELNLDKGSHWAIAGNSGSGK
                     PYALTYFLSVLKPMSGLIIIDPKFDTPSQWARENKIAVIHPVENHSKSDFVSQVNEQL
                     NQCATLIQKRQAILYDNPNHQFTHLTIVIDEVLALSEGVNKNIKEAFFSLLSQIALLG
                     HATKIHLFLGSQRFDHNTIPISVREQLNVLLQIGNINQKTTQFLFPDLDPEGIVIPTG
                     HGTGIIQVVDNEHSYQVLPLLCPTYYTKRGIL"
     gene            2755..3357
                     /locus_tag="VC122_001608"
     CDS             2755..3357
                     /locus_tag="VC122_001608"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003039416.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEP5641517.1"
                     /translation="MTTKTYFMRSFNLILYLLLIAPICYVFGWLFNLVSVQLSTVTFV
                     NLDTVKNVTDTISSVCYGFALISLLLALLSGLKMIQRLNTDSLGNYCQSIYRTFLLRH
                     FLFQRERVQKVSNFEHQTVTTINPIHNGFNRAVRKCIVDIRKDAITVFLKVPRDQQGQ
                     KILKDTGVQLKEEIVIQHTDYYFSSPVRVRNQLWFIGQKR"
     gene            complement(3833..4114)
                     /locus_tag="VC122_001609"
     CDS             complement(3833..4114)
                     /locus_tag="VC122_001609"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009880705.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator"
                     /protein_id="HEP5641518.1"
                     /translation="MLSKSLQVPLEQLIAEDIPKMTLIIKDEEIKQCDRNTHIMLFGT
                     LLIAISIYPIMSWLGWWGYGPVAILWLIIMRIALRIERFKKTYDVQTYR"
     gene            complement(4121..4165)
                     /locus_tag="VC122_001610"
                     /pseudo
     CDS             complement(4121..4165)
                     /locus_tag="VC122_001610"
                     /inference="COORDINATES: protein motif:HMM:NF013540.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
BASE COUNT         1464 a          797 c          728 g         1468 t
ORIGIN      
        1 tatgattcaa aatgttgtta cttcaataat cctgtattct gggacagccg tagacttact
       61 tattatccta atgttatttt ttgccaaaag aaaaagcaga aaagacatca ttaacatcta
      121 tttaggacaa tttctaggct ctgttagtct aatattgcta agtttgcttt ttgcatttgt
      181 cttagattat attcctagta aagagatttt aggtttgctc ggtttgattc caattttcct
      241 aggcctcaaa gttttgcttt taggagattc tgatggagaa gctattgcca aagaaggttt
      301 gagcaaagat aataaaaacc tgatttttct agtcgctatg attacttttg caagttgtgg
      361 tgctgacaat attggtgtct ttgtcccata ttttactacc ttaaatttag cgaatttgat
      421 agtggcttta cttacctttc tagtcatgat ttatctcttg gttttttctg cccaaaaatt
      481 ggcacaagtc ccttctgttg gagaaacttt ggaaaaatat agcagatggt ttattgccgt
      541 tgtctattta ggattgggga tgtatatcct gattgaaaac aacagctttg acatgctatg
      601 ggctgtgtta ggctaggaga aaaaattatg aaaaaagata gtatctgtca agtaggtgtt
      661 ataaatcaac aaaatgttac aaccgcaacg aactaccttg aaaaggaaaa agtccaaaaa
      721 tcacttcgta ttttatcaaa atttaccgat aataaacaga taaatatcat cttttatctc
      781 cttgccgtcg aagaactctg tgtctgcgat atagcctgtt tattaaatct cagtatggca
      841 tctgcctccc accatcttcg taaactagcc aatcaaaaca tcttggacac tagaagagag
      901 gggaaaatta tatattattt tataaaagat gaggaaatca gagatttttt taatcaacta
      961 ggataacaac tatttttact actttctact tttccatgat tataggggga ttatatatga
     1021 aattttttga agaaaattat tcacaagaaa tacctactcg tatcaaaaat ttaagaaaaa
     1081 aacacaatat tacacaaagc gaactaggca atgcaggtca agttagtcaa gttgaaagtg
     1141 gtaagcgacc aattacgagt tcgatgttag tttatttaaa tgctctaacc gcttcgagct
     1201 atacgtatat cgtttttgga gagttagatg agtttattga gaatctgttt cattattttt
     1261 tcagttctat tttatataga gatttagagg ctgtagatga aaacctatat tctttcatga
     1321 gtgatgattt aatttcaatc caatcaagtt gtctaagtat tgccaagaca tttgcaaatt
     1381 tcaatattca aagaaaaaga tttatgattt ctactgaaac tgaaatggat acttttcaca
     1441 agaaagatga cattgacgta tgggttggtg gaaaaagtta taaccccgct aggagtttta
     1501 ggaataatcc aataaatgaa ttaactgtta ttgattttga agaaatggct gatattcttt
     1561 tgttgatgtt gagagataat ctaattaggt cgtttgaaat taatgtctgc aacacactgt
     1621 tcgaattaga taaaaatggt gaaccgacaa catttaatct agataaaatt gactcaataa
     1681 tcaataagtg gtggtctgag aatgtctcaa cagagataat acccaacctc ataaaaaaac
     1741 ttagagaaaa tcccttgttt aatatcggat ttatggtaaa tgatatatta gaacgaatgt
     1801 ataaagagga cataccaaag tcttacctta cttctgttcc attagttatt tccaaaaaag
     1861 caaggtcaac tttatcgatt aagtcaaacg gaagtcaaaa aatagataaa tcaaaatttg
     1921 cacagatttt gtccctttag agacggatga cttacacatt caacgtggat tattcttccc
     1981 ttggagaaag gggatttcag aacgcttggc gattcctgat ttagataaat tttcatctaa
     2041 cttacaacag gggaaaattc ctgtcataaa gcacttcgaa ctcaatttag ataaggggag
     2101 tcactgggct attgctggaa attcaggttc tgggaaaccg tacgctctca cttacttctt
     2161 gagtgtcctg aaacctatgt ccggcctaat tatcattgac ccaaaattcg atacccctag
     2221 ccaatgggca agagaaaata aaattgccgt tatccaccca gtagaaaacc attcaaaatc
     2281 tgatttcgtt tcacaagtca atgaacaatt aaaccagtgt gcaactctca ttcagaaacg
     2341 acaagctatc ttgtatgata atcccaatca tcaatttacc catctaacta ttgttattga
     2401 tgaagtcctc gctctatcag agggagtcaa taagaatatc aaagaagctt ttttctccct
     2461 actatcacaa attgccttgt tgggacatgc tactaaaatc catctattct tgggaagcca
     2521 gcgttttgac cataacacta ttcccatttc agtgagggaa cagctaaacg tattattgca
     2581 aattggaaat atcaatcaga aaaccactca attcttattt ccagacctag atccagaggg
     2641 gattgtcatt ccaacaggac acggaacagg tatcattcaa gttgtcgata atgaacactc
     2701 ctatcaagtt ctacccttac tctgtccaac ctactatact aagcgaggta tcctatgacc
     2761 actaaaacct acttcatgag aagcttcaat ctcatattat acttactgct tattgctcca
     2821 atctgctatg tgttcggttg gctgttcaat cttgtttcag ttcaattatc taccgtcaca
     2881 tttgtcaatc tggatacggt taaaaacgtt actgatacca ttagttctgt atgttatgga
     2941 ttcgctctta tttctttact tctagcctta ttatctggat taaagatgat acaacgcttg
     3001 aatacagata gccttgggaa ctactgccaa tctatttatc gcacctttct attaagacac
     3061 ttccttttcc agcgtgaacg agtacagaag gtaagcaact ttgaacacca gaccgttacc
     3121 actatcaatc caatccataa cgggtttaac cgagctgtac gtaagtgtat cgtagatata
     3181 cgaaaagatg ccattactgt atttctaaaa gttcctagag accaacaggg gcaaaaaatc
     3241 ctaaaggata cgggagtaca actgaaagaa gagattgtca tccaacacac agactattat
     3301 ttctcttctc ctgttcgagt tcgtaaccaa ttgtggttta ttggtcaaaa gcgttaattc
     3361 cttggggctg tggcttgcgt taagcaaaag ccagacagcc ccttttttat attcttatct
     3421 atcatgacct attggggtta ctacgacaac gaaagttaga agctgtgggt atttatttat
     3481 cttgaaaaga ttgctgaact aacgcaataa ctactctacc attcactcta ccttttatta
     3541 agcaactatc aataaatatt gatacaataa ggtttataac agctaaaata gaaaatactt
     3601 aacctttaca gaagtaaaat aattgagatg agaaaagcct atgaaatgaa cgtttcaaag
     3661 gcttttttcg tgtttaaaaa aacaaatgaa aaaacaaaat agagagtagg gttattaaca
     3721 ctgtcaatcc tgtgtacgca ataacaatcc atgctttttg ataaggatat tttgcttttt
     3781 ctttaatctt atcaatctga tctaagctct caccctttgc tataacttca agttatcggt
     3841 aagtctgaac atcatatgtc tttttaaatc gctcaattct taaggcgata cgcatgatta
     3901 tcaaccataa gatagcaacc ggaccatatc cccaccaacc taaccaagac attataggat
     3961 aaatactgat agcaattagg agtgtcccaa ataacataat atgcgtattc ctatcacatt
     4021 gtttaatctc ctcatcctta ataattaaag tcatttttgg gatatcttct gctattagtt
     4081 gctctaaagg aacctgtaag gacttactta acatcaaaag attttgcagg tctggatagc
     4141 ttttaccgtt ttcccagttt gagatagtct tcttgaaaca tacatcattt cagcaagctc
     4201 ttcttgtgac atctgtaatt tttttcaata ataagataac tggtttttaa agcccataat
     4261 aaatctcctt tttaatagct actataatcc ttcttcaata agtttactat caaaagtctt
     4321 tgacctaagc tttttttcgt ttatcttttt aataattatc gtaaggttta cagtctttgt
     4381 aaattttatt tacaactttg acaatcaaac ttgacttgat cacaaagaca tggtgtttaa
     4441 ggaaaatatc ttcaaaa
//
LOCUS       DATXIG010000087         4342 bp    DNA     linear   BCT 28-DEC-2023
DEFINITION  TPA_asm: Streptococcus pyogenes strain MGAS11113
            SAMN08555799-rid19682583.denovo.087, whole genome shotgun sequence.
ACCESSION   DATXIG010000087 DATXIG010000000
VERSION     DATXIG010000087.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN08555799
            Sequence Read Archive: SRR6740495
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pyogenes
  ORGANISM  Streptococcus pyogenes
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 4342)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4342)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-OCT-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-OCT-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001918058.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001918058.2
            Genome Coverage       :: 368x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/12/2023 11:23:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 1,806
            CDSs (total)                      :: 1,769
            Genes (coding)                    :: 1,722
            CDSs (with protein)               :: 1,722
            Genes (RNA)                       :: 37
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1, 1 (5S, 23S)
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 47
            CDSs (without protein)            :: 47
            Pseudo Genes (ambiguous residues) :: 0 of 47
            Pseudo Genes (frameshifted)       :: 30 of 47
            Pseudo Genes (incomplete)         :: 20 of 47
            Pseudo Genes (internal stop)      :: 9 of 47
            Pseudo Genes (multiple problems)  :: 11 of 47
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4342
                     /organism="Streptococcus pyogenes"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN08555799-rid19682583.denovo.087"
                     /strain="MGAS11113"
                     /serotype="Emm28"
                     /isolation_source="normally sterile site"
                     /host="Homo sapiens"
                     /db_xref="taxon:1314"
                     /country="Finland"
                     /collection_date="2002"
                     /collected_by="National Public Health Laboratory"
     gene            complement(<1..160)
                     /locus_tag="VC122_001611"
     CDS             complement(<1..160)
                     /locus_tag="VC122_001611"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017769756.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="portal protein"
                     /protein_id="HEP5641519.1"
                     /translation="MGLLDLIGRKRARDKPQNSYEGQDFFYLFGRTTSGENVDEFKGI
                     KVEFIQLSN"
     gene            complement(265..537)
                     /locus_tag="VC122_001612"
                     /pseudo
     CDS             complement(265..537)
                     /locus_tag="VC122_001612"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000424370.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            complement(556..861)
                     /locus_tag="VC122_001613"
     CDS             complement(556..861)
                     /locus_tag="VC122_001613"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011889070.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelE/ParE family
                     toxin"
                     /protein_id="HEP5641520.1"
                     /translation="MKEYQVTMSDDAKADLLSIYHYVRDELCAPQAADNLLERLSQEM
                     LSLSIFPERCAIIEDLIGKGYTFRQLIVKKYRIVYHVLEDEVIIVAVVYGSRHMNNW"
     gene            complement(861..1163)
                     /locus_tag="VC122_001614"
     CDS             complement(861..1163)
                     /locus_tag="VC122_001614"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001140389.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelB/DinJ family
                     antitoxin"
                     /protein_id="HEP5641521.1"
                     /translation="MAKTGTLNLRVDDSVKSAADDILKRLGIPMSTAIDMFLNQIILT
                     GGIPFDVSLPEAPQRVNVDYMSQEKFYDKLIFSFEDAKTGNRKDVREFLSQLKENA"
     gene            complement(1218..1394)
                     /locus_tag="VC122_001615"
     CDS             complement(1218..1394)
                     /locus_tag="VC122_001615"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011054282.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="terminase"
                     /protein_id="HEP5641522.1"
                     /translation="MGQNVVMRQAPGGNIKPDKEKSVEKIDGIVGLILGLDRCIRRQG
                     DEASVYDERGILSF"
     gene            1410..2303
                     /locus_tag="VC122_001616"
     CDS             1410..2303
                     /locus_tag="VC122_001616"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019418791.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEP5641523.1"
                     /translation="MTTMLDFPFHHEFIEFLGRWTHILITLLKRHHGQADTAIPRYLL
                     VTIREKTGIVKSTTVQNTAFTTQSNQLKKQYGQFLLTESNNCCACPGCDRPLILTSGN
                     LAAENYEVSAIEKDKDAEPLNLIALCPDCFLTYQADSRKKIVTALKNVKKILVSAHNS
                     QQAISDMKLDSGIVSVLTSLNKLKCDEYDISYDSKQLTDKISPENNRTLYQLVKNPVI
                     DNYLTIQKIIVNLDKQGKIDYEEIQYQTRSMYKKLKAAKHNNLAIFNTISEKLHKATL
                     QDIYFCQTIVSYFIQKCEVLE"
     gene            2303..2527
                     /locus_tag="VC122_001617"
     CDS             2303..2527
                     /locus_tag="VC122_001617"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001176804.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEP5641524.1"
                     /translation="MQLPNKLYSYQKSTLAYLPRVLNEIKSGNSNVKDIFHAISEELD
                     DPTDFLSIMDCLYALNAIEMSNEGEVKLCL"
     gene            2518..4176
                     /locus_tag="VC122_001618"
     CDS             2518..4176
                     /locus_tag="VC122_001618"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000904398.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2326 domain-containing protein"
                     /protein_id="HEP5641525.1"
                     /translation="MLIEMWSPVFKNHGEIRESICFHYGLNVIMGMDLADNSIGKSSS
                     LLAIDFIFGGNSYLKSIAVKKLGDHPIYFCFQFEKKFYFSRDTANPDIILVCDKSYSP
                     TGETMDLGIFLNKLKKRYHLDSPELSFRLAMSGFFRIAGKSNQNTDFPLQVYSSQKSS
                     ESITTLIQLFNLYDNIARYKERLKDKSDQLTTFRNARKYTFISNLVGGKKQFEANVSE
                     IKRLEYDLAHLQDTHQDKIDSDDIEKNQQKLQLRNTKLELESSLRDKQRRLKLLDISI
                     EFGLYPTESDLTELQQYFPDTNLKKLYEVEAYHKKLETILDSEFSTERESLIAEIDEL
                     ESQLTTLNQELQELGNIPNLSSEYLESYSKLTATINALKEQNEAYLKESGLSKEKSEA
                     DADLKRSTEDILLDLEVKINSKMREFNNILYPDIRKAPQINLNAHNSYSFYTPDDDGT
                     GTKFKGMIVFDLVMLYLTNLPALTHDSLLLSNVSYQATEALLKLYDQSRSLNKQVFLA
                     FDKASSYSPDANQLLSENTVLRLSSNGNELYGISWNKGENSDEI"
     gene            4166..>4342
                     /locus_tag="VC122_001619"
     CDS             4166..>4342
                     /locus_tag="VC122_001619"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002985621.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HEP5641526.1"
                     /translation="MRFSYNKLWKLLIDKGWTKTKLRQEAGISSSTIAKLGKGENITT
                     DILLEICIALDCKIE"
BASE COUNT         1453 a          892 c          727 g         1270 t
ORIGIN      
        1 tgttagacag ttgaataaat tctactttaa ttcctttaaa ctcatccaca ttctcgccac
       61 tagtcgtgcg accaaagagg tagaaaaagt cctgaccttc ataactgttt tgaggcttat
      121 ctctagccct ttttcttcca attaaatcaa gtagtcccat aagccctcct tattttgggg
      181 tacaaaaaaa gcacctcctt tgaagtgctt tcggtatatt tctttcaaag taatcaacag
      241 tctttcttac aaaacctagt attctcattc tgtcaattcc ttaaatgcgt caagatgtcg
      301 tgataatact gaagtcgcaa tctcatctaa ccttgcttgt tcaacaactg ttgtcatctg
      361 ctcatcactg cttaaacttt ggtgatccgc tgatagtttg ctatcagatg gacttaatac
      421 caaagttcca tcagttgatt tataaaggtt aaactcctaa ttctcttgaa tgccaaatgg
      481 gtcaggtatc ggaagataga tgtcatcacc aatttgtatc gtctttacga gttccataca
      541 tcactctccc ttactttacc aattattcat atgacgtgat ccatagacga ccgcaacaat
      601 tataacttca tcttccagaa catggtatac aatgcgatat tttttgacaa tcagttgtct
      661 gaaggtatag ccttttccaa tcagatcctc aatgatggca caacgttcag gaaaaatgga
      721 tagtgataac atttcttggg ataacctctc aagaagatta tccgctgcct gtggtgcaca
      781 gagttcatca cgaacatagt gatagatgct cagcaaatct gctttagcat catccgacat
      841 ggtgacctga tattctttca ttaagcattt tccttcaatt gagatagaaa ttctctgaca
      901 tccttgcggt tgccagtttt cgcatcttca aagctgaaga taagcttatc ataaaacttc
      961 tcctgactca tgtaatcaac attaactcgt tgaggcgctt caggtagaga aacatcaaat
     1021 gggatacccc cagtcaaaat aatctgattc aaaaacatat caatcgcagt tgacatagga
     1081 atacctaagc gtttcaaaat atcatctgcc gcacttttta ctgaatcatc aactcgtaaa
     1141 ttcaaagttc ccgttttagc catggttatc ctcctttttt gtaacgacat tgtatcacgt
     1201 ttttagagtt gactcaacta aaaactcaat atccctcgct catcatagac gctagcttca
     1261 tcaccttggc ggcgaatgca acggtcaagc cccaggataa gacctacaat accgtcaatc
     1321 ttctcaacag atttttcctt atctggcttg atgttgcctc caggggcttg tcgcatcacc
     1381 acgttttgtc ccatccattt aaggactgga tgaccaccat gttagatttt cccttccatc
     1441 atgagtttat agagttcctt ggacggtgga ctcatatcct tataactctg ctcaaaaggc
     1501 accatggtca agccgacacc gcaattccaa ggtatcttct agtaaccata agagagaaga
     1561 cagggattgt aaaatcaacc actgtgcaaa atactgcgtt cactacccag tcgaatcagc
     1621 tcaaaaaaca atacgggcag ttcttgttaa cagaatctaa taactgttgt gcatgcccag
     1681 gctgtgatag accactcatt ctaaccagtg gcaacttagc agcagaaaat tacgaggttt
     1741 cagccattga aaaagataag gacgctgaac cactgaatct gatagccctc tgcccagatt
     1801 gttttttaac ctatcaagca gatagtcgca aaaaaattgt aacagctcta aaaaatgtta
     1861 aaaagattct agtctctgca cataacagcc aacaagcgat ttctgatatg aagttagaca
     1921 gtggaattgt ttcagttttg actagtctca ataaactaaa atgtgatgaa tatgatatct
     1981 cctatgactc gaagcaattg actgataaga tatcacctga gaacaatcgt acgctttatc
     2041 aattggttaa aaacccagtt attgataact acttaaccat tcaaaaaatc atcgtaaatt
     2101 tggacaaaca aggaaaaatt gattacgagg aaatccaata tcaaacgcga tcgatgtata
     2161 aaaagttaaa agcagccaag cataataatc tggctatttt caatactatt tcggaaaaac
     2221 tccataaagc tacactacag gacatctact tctgtcagac gattgtctct tactttatcc
     2281 aaaaatgcga ggtacttgaa taatgcaatt accaaataaa ctttactcct atcaaaaaag
     2341 cacccttgct tatttgccta gagtgctaaa tgaaatcaaa agtggaaact caaacgtcaa
     2401 agatatcttt cacgctatct cagaagagtt ggatgatcct acagatttct tatctataat
     2461 ggattgcttg tatgcattga acgctattga aatgtctaat gaaggagaag taaaattatg
     2521 cttatagaaa tgtggtctcc agtatttaag aatcacggag aaattcggga atcaatctgc
     2581 ttccactatg ggttaaacgt gattatgggg atggatttag ctgataactc tatcggcaaa
     2641 tcctcctcac tattagccat tgatttcatc tttggaggta atagctatct aaaatcaatt
     2701 gcggttaaaa aattaggtga tcatcctatc tatttctgct ttcaatttga gaaaaagttt
     2761 tatttttcaa gagatacggc caacccagat atcattctag tgtgtgacaa gagctactct
     2821 ccaaccggtg aaacaatgga cctaggaatt tttcttaaca aacttaagaa acgatatcat
     2881 ttagattctc cagagctatc attccgatta gctatgagtg gcttctttag gattgcagga
     2941 aaaagtaatc aaaataccga tttcccttta caagtttatt cgagccaaaa atcttctgaa
     3001 tctattacta ctctcatcca actgtttaat ttgtatgata acattgcacg atacaaagaa
     3061 cgtctgaaag ataagagcga ccagttaaca acttttcgta atgctcgaaa gtataccttc
     3121 atctccaacc tggttggcgg taaaaagcaa tttgaagcaa atgtctcaga aataaaaaga
     3181 ttggaatatg atttagcaca tctccaagac actcatcaag ataaaattga ctctgatgat
     3241 attgagaaaa atcaacaaaa actccagctt agaaatacta agctggagtt agaaagcagt
     3301 cttcgggaca agcaaagacg tcttaagcta ttggacatca gtatcgaatt tggcttatat
     3361 cctactgaat ctgacttaac agagcttcaa caatacttcc cagatactaa tctcaaaaaa
     3421 ttatatgaag ttgaagctta ccacaaaaaa ttggaaacta ttttagattc cgaattctca
     3481 actgaacgtg aatccctaat agctgagatc gacgaattag agagtcaatt aacaactcta
     3541 aaccaagaac ttcaagaact aggaaacatc ccaaatcttt caagtgagta cttagaaagc
     3601 tactctaaac taacagctac aatcaatgca ctaaaagaac aaaacgaggc ctacttaaag
     3661 gagtctggtc tatccaaaga aaaatcagaa gcggacgctg acttgaaacg cagtacagaa
     3721 gatatccttc tagatctaga agtaaaaatc aacagtaaga tgcgtgaatt caataacatt
     3781 ctatatccag atattcgcaa agctcctcaa attaacctca atgctcacaa tagctactcc
     3841 ttctatacac cagatgatga tggtacagga actaagttta aagggatgat tgtattcgac
     3901 ttagtaatgc tctaccttac taatctgcca gcactcacac acgattcatt actcttgagt
     3961 aatgttagct accaagcgac tgaagcactt ttgaagctgt atgaccagtc aaggtcattg
     4021 aacaaacaag tctttttagc cttcgataaa gccagctctt attctccaga tgctaatcaa
     4081 ctcttgtcag aaaatacggt attgagactc tctagtaacg gaaatgaact ctacggtatt
     4141 tcatggaaca aaggagaaaa ctcagatgag atttagttac aataaattat ggaaacttct
     4201 tattgataaa ggttggacca aaacaaagct caggcaagaa gctgggatta gttcttccac
     4261 gatcgcaaaa ttgggaaaag gggaaaatat cactactgat attttattgg aaatttgtat
     4321 tgcacttgat tgtaagattg aa
//
LOCUS       DATXIG010000088         4253 bp    DNA     linear   BCT 28-DEC-2023
DEFINITION  TPA_asm: Streptococcus pyogenes strain MGAS11113
            SAMN08555799-rid19682583.denovo.088, whole genome shotgun sequence.
ACCESSION   DATXIG010000088 DATXIG010000000
VERSION     DATXIG010000088.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN08555799
            Sequence Read Archive: SRR6740495
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pyogenes
  ORGANISM  Streptococcus pyogenes
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 4253)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4253)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-OCT-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-OCT-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001918058.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001918058.2
            Genome Coverage       :: 368x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/12/2023 11:23:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 1,806
            CDSs (total)                      :: 1,769
            Genes (coding)                    :: 1,722
            CDSs (with protein)               :: 1,722
            Genes (RNA)                       :: 37
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1, 1 (5S, 23S)
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 47
            CDSs (without protein)            :: 47
            Pseudo Genes (ambiguous residues) :: 0 of 47
            Pseudo Genes (frameshifted)       :: 30 of 47
            Pseudo Genes (incomplete)         :: 20 of 47
            Pseudo Genes (internal stop)      :: 9 of 47
            Pseudo Genes (multiple problems)  :: 11 of 47
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4253
                     /organism="Streptococcus pyogenes"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN08555799-rid19682583.denovo.088"
                     /strain="MGAS11113"
                     /serotype="Emm28"
                     /isolation_source="normally sterile site"
                     /host="Homo sapiens"
                     /db_xref="taxon:1314"
                     /country="Finland"
                     /collection_date="2002"
                     /collected_by="National Public Health Laboratory"
     gene            75..758
                     /locus_tag="VC122_001620"
     CDS             75..758
                     /locus_tag="VC122_001620"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014635524.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HEP5641527.1"
                     /translation="MKITKATEITKTHNWRILIYGKPGNGKTYLTNYLKGKTLILDMD
                     HSSKTIAGNENIDIIQFDRTHPSDFMTEFLTELPELIKEYDNLVIDNITSWQSDWFIE
                     QGRKSKNGITNELQQYNMWTNYYLRVLTTIYSQPTNIFVTAWESTQDLTLESGQIITQ
                     YVPDIRKQVLSQTLGLTDVVGRIQVNPKTGGHGILLQGSDGLYAKNRLDNRTVCKAEE
                     LFNFEGSDA"
     gene            794..2197
                     /locus_tag="VC122_001621"
     CDS             794..2197
                     /locus_tag="VC122_001621"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017770133.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase"
                     /protein_id="HEP5641528.1"
                     /translation="MNEARRHILKHNVMIVSPPGSGKSVVISDIAKSATQKNGHVLFL
                     VHRKELIDQITNSFKFHGIDMNKVDLMTVGKAKNRLDKLTKPTLIITDEGHHGKASTY
                     QIIYEYFSDVPRIGFTATPWRLSGDGFTDTYDVMVLGKTVEWLINNNKLAPYDYYSVL
                     SIDTAKLKVQNGDYSNKSIDESFGKKIFGDVVQEYIKKANGQKAILYAHSVEASQAFA
                     KEFQSMGINAIHADAKTPKAKRDKIMKDFRDGKIQVICNVDLISEGFDVPDCTVTILC
                     RPTKSLVLFLQQSMRSMRYQPNKKAIILDHVGNWNIHGLPDTPHHWENYFRGGWKKKS
                     NKTNTVHAKECSVCSALWPLSQQLCELCNHDFGLKEKQEKERIEAELELIKRERFRIK
                     QLANKKFGKDLKTNWEIAQARVKDAGKGKPLYKLIYFYLKTDWVETNVNELAEVTGKS
                     EKEIYSAYNWLKKKLRG"
     gene            2202..2684
                     /locus_tag="VC122_001622"
     CDS             2202..2684
                     /locus_tag="VC122_001622"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000896928.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF669 domain-containing protein"
                     /protein_id="HEP5641529.1"
                     /translation="MAGFTTDFSEVKEHAEFKEQPYEMIVYDAYEAVNERNGKKRVVI
                     DYVVRNDIKQEMQNYHLWDEQYPNSQTGKYHIGILMGKAKALGIKEGQHYDSFEAFLN
                     DFKGRTAKVTVKLDEYNGNKYPKVRYANQSDVPNSYHVWKEKATGFTQAEIEEDDLPF
                     "
     gene            2702..>4253
                     /locus_tag="VC122_001623"
     CDS             2702..>4253
                     /locus_tag="VC122_001623"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011017876.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage resistance protein"
                     /protein_id="HEP5641530.1"
                     /translation="MTSNEFIEALSKLTTETDWGDPIFGESVLKAELRKHLFKIVPID
                     HNGYIHKLFYSEMVKDEDVMYFVSDGRKTYRFLFGDTILKTDKQGNEYLTYSVENNFP
                     PFAKLVIDYILGAYTFFENKLYDIRYKQFKLIDDFTLQTKYGFKDSGHILEILQGIHK
                     TLNIQPINYIEPYQIACKDFIIDLENSEIINQPPLQNVSYFKYYEVDYKTAINSKSIA
                     EEYLEYVIADSNSLNNAILQSYFIAQVACGVRPKTNFFISKSGVRTGKGLRHIALSGL
                     FNKIDVELDTLKSNGFEALQAWAMFSGGEMALATEQGDIQGNAMERVLKIIATEKTHV
                     ARAIGQNQSMVNLTSVLCIDTNRTVALSDEMNGRKVLIQFKDRPKTETDYERESIFRK
                     YWLAFTDCDKNPKIDGCIGFLLNSLERFQKIGKWYQWKDVEVFNDIDLDEFQVALINA
                     LQEVDFVQRTDNKEVIDLSLQVYGKSNHALSKAISEIGVRSRSKKVNGKTVRGYEIEN
                     KTRFDKYIL"
BASE COUNT         1568 a          698 c          833 g         1154 t
ORIGIN      
        1 gggaaagaag aagatattag aaaaatgatt gattacgatg ataaatttac cgaacttttt
       61 ggagggatta gacattgaag attactaaag cgacagaaat tacaaaaacc cataattggc
      121 gcatattaat ctatggtaaa cccggaaacg ggaaaactta cttaactaat tatttaaaag
      181 gcaagacact gattttagac atggaccatt cgtctaaaac gattgccgga aacgaaaaca
      241 ttgatattat ccaatttgac aggacgcacc ctagcgactt tatgaccgaa ttcttgacag
      301 aactaccaga acttatcaaa gaatatgaca atttagtcat tgataatata acaagttggc
      361 aatcagactg gtttatcgaa caaggtcgta aatccaaaaa tggaatcaca aacgaattgc
      421 aacaatacaa catgtggacc aattactatt tacgagtatt gactaccatt tacagccaac
      481 ctactaatat ttttgtgact gcttgggaat caacgcaaga tttaacgctc gagagcggac
      541 aaattatcac gcaatacgtg ccagatatcc gcaaacaagt cctaagccaa acgctaggtt
      601 taaccgatgt tgtcggacgt attcaagtaa atccaaaaac gggcgggcat ggaattttgt
      661 tgcagggcag cgacggacta tatgcaaaaa ataggcttga taataggacc gtttgtaagg
      721 cggaagagtt gtttaatttc gaagggagtg atgcgtaacg gtataccatt tacacgaata
      781 tcaaacagaa cttataaatg aggcaagaag acatatttta aagcataatg tgatgattgt
      841 tagtccacct ggaagtggta agtcagtagt catttctgat attgctaagt cagcgactca
      901 aaaaaacgga cacgttttat tcttggtcca tcgcaaagaa ttaattgacc aaatcactaa
      961 tagttttaaa ttccatggaa ttgacatgaa taaagtggat ttaatgacag ttggtaaagc
     1021 taaaaaccgc ttggataaat taacaaaacc aaccttaatt atcacagatg agggtcatca
     1081 tgggaaagct agtacctacc aaataatcta tgaatacttt tcagacgtgc cgaggattgg
     1141 ttttactgcc acaccttggc gtctatcagg cgacggtttt acagacactt acgatgttat
     1201 ggttcttggt aaaacggtcg agtggcttat caataataat aaacttgcac catatgatta
     1261 ttacagtgtt ctatcaattg atactgcgaa attaaaagta caaaacggag attactccaa
     1321 taaatcgatt gacgaatcat ttggtaaaaa gatttttggt gatgtagttc aagaatatat
     1381 aaaaaaagcg aacggtcaga aagctatttt atacgctcac tcggtagaag catcgcaggc
     1441 atttgctaaa gaattccaat ctatgggaat taatgcaata cacgcagatg caaaaacgcc
     1501 taaagccaaa cgggataaaa tcatgaaaga tttccgtgac ggtaagatac aagttatctg
     1561 taacgttgat ttgatatcag aaggtttcga tgtcccagat tgtacagtaa ctattctttg
     1621 tagacctaca aaatcattag tattattttt gcaacaatct atgcggtcga tgagatatca
     1681 acctaataaa aaagccatca ttttagatca cgtaggaaat tggaatattc acggcttacc
     1741 tgacacaccg catcattggg agaattattt ccgaggaggg tggaagaaga agtcgaataa
     1801 aactaacacg gtacacgcaa aagaatgttc tgtgtgttcg gctttgtggc cacttagtca
     1861 acagctctgt gaattatgca atcatgattt tggattgaaa gaaaaacaag agaaagaacg
     1921 catagaggca gaacttgaac tcataaaacg tgagcggttt agaatcaaac aacttgctaa
     1981 taagaagttt ggtaaagatt taaaaacaaa ctgggaaatt gcccaagcta gagttaaaga
     2041 cgctggtaaa ggaaaaccat tatataaact tatctatttc tacttaaaaa ctgattgggt
     2101 agaaacaaac gttaatgaac ttgccgaagt aacaggcaag tcagaaaaag aaatatatag
     2161 cgcctacaat tggctaaaaa agaaattaag aggataaaaa catggcagga tttacaacag
     2221 atttttcaga agttaaagaa cacgcagaat tcaaagaaca accatacgaa atgattgtct
     2281 atgatgcata tgaagcagta aatgaacgta acggcaaaaa acgtgttgtt attgactacg
     2341 ttgttcgaaa cgatatcaag caagaaatgc aaaactacca cttatgggat gagcaatatc
     2401 ccaactcaca aactgggaag tatcatatcg gcatcttaat gggtaaagca aaagcacttg
     2461 gtatcaaaga ggggcaacac tacgatagtt ttgaagcgtt tttaaacgac ttcaaaggac
     2521 gcactgcaaa agtaaccgtt aaacttgacg aatataacgg aaataaatac ccgaaagttc
     2581 gttacgcaaa tcaaagcgat gtgcctaaca gctaccacgt atggaaagaa aaagcaactg
     2641 gatttacaca agcggagatt gaagaagacg atctaccgtt ctaatttaga ctgggggata
     2701 aatgacatca aatgaattta ttgaagcttt atcaaaattg acaaccgaaa ccgattgggg
     2761 agacccaatc ttcggcgagt cagtgcttaa agctgaatta cgaaaacact tatttaaaat
     2821 tgtcccaatt gatcacaatg ggtatatcca caaactgttt tattcagaaa tggttaaaga
     2881 tgaagatgtc atgtattttg tgtcagatgg acgaaaaact tatcgctttt tatttggaga
     2941 tacaattcta aagactgata aacaaggcaa tgaatatctc acatactccg ttgaaaataa
     3001 ttttccacca tttgcaaaac tagttatcga ctacattcta ggtgcttaca cgttttttga
     3061 gaataaactt tatgacattc gatataagca atttaaattg attgatgatt ttacacttca
     3121 gactaagtat ggtttcaaag actctggtca cattttagag atactacaag gtatccacaa
     3181 aacattaaac atccaaccaa tcaattatat tgaaccatat cagattgctt gcaaggattt
     3241 cataatcgac cttgaaaatt ctgaaatcat taaccaaccg cctttgcaaa acgtatctta
     3301 ttttaagtat tacgaagtag actacaaaac agcaataaac agtaagtcta ttgcagaaga
     3361 atatcttgag tacgttattg cagatagcaa ttcgttaaac aatgcaatac tacaatctta
     3421 ttttatcgcc caagtagcgt gtggtgttag acctaaaacc aacttcttca tctcaaaatc
     3481 tggagtaagg actggtaaag ggttaaggca tatagcttta tctggtctat tcaataagat
     3541 tgatgttgag ctagatacat taaaaagtaa tggatttgaa gcgttacagg cgtgggcgat
     3601 gttctcgggt ggagaaatgg ctctagcgac ggaacaggga gatattcaag gcaatgcaat
     3661 ggagcgtgta cttaaaatta ttgcaacaga gaaaacacac gttgcacgag caattgggca
     3721 aaatcagtca atggttaatt taacgagtgt tctgtgtatt gacaccaacc gtactgttgc
     3781 gctgtcggat gaaatgaatg gacgaaaggt tttaattcag tttaaagata gaccaaaaac
     3841 tgaaactgat tatgaaagag agagcatatt caggaaatat tggctagcat ttactgactg
     3901 tgataaaaat ccaaaaatcg atggatgtat cggttttctg ctaaattcac tcgagcgttt
     3961 ccaaaaaatt ggtaaatggt atcaatggaa agatgttgaa gtatttaatg atatcgattt
     4021 agacgaattt caagttgctt taataaatgc attacaagaa gttgattttg tacagcgaac
     4081 tgataacaaa gaagttattg acttatcatt acaagtttac ggaaaaagca atcatgcatt
     4141 aagtaaagcc atatctgaaa ttggtgtacg tagcaggtcg aagaaagtta acgggaaaac
     4201 agtcagaggg tatgaaatcg aaaataaaac acgttttgat aaatatatcc ttt
//
LOCUS       DATXIG010000089         4241 bp    DNA     linear   BCT 28-DEC-2023
DEFINITION  TPA_asm: Streptococcus pyogenes strain MGAS11113
            SAMN08555799-rid19682583.denovo.089, whole genome shotgun sequence.
ACCESSION   DATXIG010000089 DATXIG010000000
VERSION     DATXIG010000089.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN08555799
            Sequence Read Archive: SRR6740495
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pyogenes
  ORGANISM  Streptococcus pyogenes
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 4241)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4241)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-OCT-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-OCT-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001918058.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001918058.2
            Genome Coverage       :: 368x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/12/2023 11:23:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 1,806
            CDSs (total)                      :: 1,769
            Genes (coding)                    :: 1,722
            CDSs (with protein)               :: 1,722
            Genes (RNA)                       :: 37
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1, 1 (5S, 23S)
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 47
            CDSs (without protein)            :: 47
            Pseudo Genes (ambiguous residues) :: 0 of 47
            Pseudo Genes (frameshifted)       :: 30 of 47
            Pseudo Genes (incomplete)         :: 20 of 47
            Pseudo Genes (internal stop)      :: 9 of 47
            Pseudo Genes (multiple problems)  :: 11 of 47
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4241
                     /organism="Streptococcus pyogenes"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN08555799-rid19682583.denovo.089"
                     /strain="MGAS11113"
                     /serotype="Emm28"
                     /isolation_source="normally sterile site"
                     /host="Homo sapiens"
                     /db_xref="taxon:1314"
                     /country="Finland"
                     /collection_date="2002"
                     /collected_by="National Public Health Laboratory"
     gene            <1..173
                     /locus_tag="VC122_001624"
     CDS             <1..173
                     /locus_tag="VC122_001624"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002992583.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HEP5641531.1"
                     /translation="LIHPYLTTFDINVENLGKMSFKQLLDIINSNEQNLSQTIFVPFS
                     LKQRESVRDISH"
     gene            289..1782
                     /gene="malQ"
                     /locus_tag="VC122_001625"
     CDS             289..1782
                     /gene="malQ"
                     /locus_tag="VC122_001625"
                     /EC_number="2.4.1.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014635535.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-alpha-glucanotransferase"
                     /protein_id="HEP5641532.1"
                     /translation="MNKRASGILMHISSLPGKFGIGTFGKSAFEFVDFLAETKQTYWQ
                     ILPLTTTSFGDSPYQSFSAIAGNTHFIDFELLVDDELLEAADLCDITFGTNPEAVDYA
                     QLFQVRRPLLEKAVRAFVAEQENVCKLEAFETASSWLTDFAEFMALKEYFNNKALQDW
                     DDETVIKRQEDSLNNYRELLAKKITYHKVCQYFFYQQWSALKTYANHKGIEIIGDMPI
                     YVSADSVEVWTMPELFKVDSDKKPLFIAGVPADGFSEDGQLWGNPTYNWSAHEKSNFA
                     WWIYRIQESFKLYDQLRIDHFKGFSDFWEIPAGDKTARNGHWASAPGIALFSAVREAL
                     GELPIIAENLGYIDEKAEQLLASTGFPGMKILEFGLFDITSQSIDLPHYYDRNCVAYT
                     GTHDNEVVNGWYDNLSEEQVHFVNNYLHKHADESITKAMLRTIFASVCDTAILCIQDL
                     LDKDGKSRMNMPNTIGGNWQWRMLDGELNQDHKDYLIYLTDLYGRAR"
     gene            1817..4081
                     /gene="glgP"
                     /locus_tag="VC122_001626"
     CDS             1817..4081
                     /gene="glgP"
                     /locus_tag="VC122_001626"
                     /EC_number="2.4.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002992581.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycogen/starch/alpha-glucan family
                     phosphorylase"
                     /protein_id="HEP5641533.1"
                     /translation="MTRFTEYVETKLGKSLTQASNEEIYLSLLNFVKEEASHKAKNTA
                     KRKVYYISAEFLIGKLLSNNLINLGIYKDIKEELAAAGKSIAEVEDVELEPSLGNGGL
                     GRLASCFIDSISSLGINGEGVGLNYHCGLFKQVFKHNEQEAEPNFWIEDDSWLVPTDI
                     SYDVPFKNFTLKSRLDRIDVLGYKRDTKNYLNLFDIEGVDYGLIKDGISFDKTQIAKN
                     LTLFLYPDDSDKNGELLRIYQQYFMVSNAAQLIIDEAIERGSNLHDLADYAYVQINDT
                     HPSMVIPELIRLLTEKHGFDFDEAVAVVKNMVGYTNHTILAEALEKWPTAYLNEVVPH
                     LVTIIEKLDALVRSEVSDPAVQIIDESGRVHMAHMDIHFATSVNGVAALHTEILKNSE
                     LKAFYDLYPEKFNNKTNGITFRRWLEFANQDLADYIKELIGDEYLTDATKLEKLMAFA
                     DDKAVHAKLAEIKFNNKLALKRYLKDNKGIELDEHSIIDTQIKRFHEYKRQQMNALYV
                     IHKYLEIKKGNLPKRKITVIFGGKAAPAYIIAQDIIHLILCLSELINNDPEVSPYLNV
                     HLVENYNVTVAEHLIPATDISEQISLASKEASGTGNMKFMLNGALTLGTMDGANVEIA
                     ELAGMENIYTFGKDSDTIINLYATASYVAKDYYDNHPAIKAAVNFIISPELLAFGNEE
                     RLDRLYKELISKDWFMTLIDLEEYIEVKEKMLADYEDQDLWMTKVVHNIAKAGFFSSD
                     RTIEQYNEDIWHSR"
BASE COUNT         1400 a          745 c          813 g         1283 t
ORIGIN      
        1 aattaattca cccttattta acgacatttg atataaatgt tgaaaatctt ggaaaaatgt
       61 cttttaagca attacttgat attatcaatt ccaatgaaca gaatcttagt cagactattt
      121 ttgttccttt tagtttaaaa caaagagaaa gcgtccgtga tattagtcat taagagcaaa
      181 aaaaagtagc atttttaagg aatgttactt tttttaggaa actggttgac ttacgcaagc
      241 gcttgcgtta taatctcatc atgaatagat ttcacaagga gaaaaatcat gaacaaacgt
      301 gcaagcggta tcttaatgca tattagttcc ctgcctggga aatttgggat aggcactttt
      361 ggaaaatcag cttttgaatt tgttgatttt ctagcagaga cgaaacaaac ctattggcaa
      421 attctgcctt taacaacgac gagttttgga gattctcctt atcagtcatt ttcagctatt
      481 gctgggaaca cacatttcat tgattttgaa ttgctagtag atgatgaact tttagaagca
      541 gcagatttgt gtgatattac attcggcaca aatcctgaag cagtagacta tgctcagctt
      601 tttcaagtta ggcgtccact tttagaaaaa gcagtgagag cttttgttgc tgaacaagaa
      661 aatgtgtgta aattagaagc ttttgaaaca gcttctagct ggttaactga ttttgctgaa
      721 tttatggctt taaaagaata ttttaacaat aaagccttac aagattggga cgacgaaact
      781 gtcattaaac gccaagaaga ctcacttaat aattaccgtg agttgcttgc aaaaaagatt
      841 acctatcata aagtctgtca atacttcttt taccaacaat ggtcagcctt gaaaacttac
      901 gctaaccata aagggataga aattattggt gatatgccta tttatgtttc tgcggacagt
      961 gtagaagttt ggacgatgcc tgagctattt aaagtagata gtgataaaaa gccattgttt
     1021 attgcaggtg taccagcaga tggctttagt gaagatggtc agctgtgggg aaatccaacc
     1081 tacaattggt ctgctcatga aaagtctaat tttgcatggt ggatttatcg tattcaagaa
     1141 agtttcaaac tatatgatca gctaagaatc gatcatttta aaggcttctc agatttttgg
     1201 gaaattcctg ctggtgataa aactgctaga aatggccatt gggcctcagc cccaggtata
     1261 gcattatttt ctgcggttcg tgaggcctta ggagagctcc ctatcatcgc agaaaacctt
     1321 ggttatattg atgaaaaagc agagcagcta ttagcatcaa caggctttcc tggcatgaag
     1381 atattagaat ttggtttatt tgatataacg agtcaaagta tagatttacc acattactat
     1441 gaccgtaatt gtgtcgctta cacagggact catgataacg aggtggttaa cggatggtat
     1501 gacaatctta gtgaagaaca agtacacttt gtaaataact atcttcacaa acatgcagat
     1561 gaaagtatta caaaagccat gctaagaaca atctttgctt ctgtttgtga tacagccatt
     1621 ctttgcatcc aagatttatt agataaagat ggtaagagtc gtatgaatat gccaaataca
     1681 attggaggta attggcaatg gcgtatgctt gacggtgaat tgaaccaaga tcacaaagat
     1741 taccttattt atttaacaga tctttatggt cgcgcaagat aatatttaaa tgttacatta
     1801 ttgaaaagag gaagacatga cacgctttac agaatatgtt gaaactaaac taggaaaatc
     1861 acttacgcag gctagcaatg aagagattta tctatcatta ttaaactttg tcaaagaaga
     1921 agctagccac aaagctaaaa atactgccaa acgcaaagtt tactatatct cagcggaatt
     1981 tttgattggt aaattactat caaacaacct gattaaccta ggaatttata aagatatcaa
     2041 agaagaattg gcagctgctg gcaaatctat cgcagaagtc gaagatgttg aattagaacc
     2101 atcactaggt aatggtggtt taggacgcct tgcttcatgt ttcattgact ctatttcatc
     2161 tcttgggatt aatggtgaag gggttggttt aaactatcat tgtgggttgt ttaagcaagt
     2221 cttcaaacac aatgagcaag aagctgagcc aaacttctgg attgaagatg actcatggtt
     2281 ggttccaaca gacatttctt acgatgtgcc ttttaagaac ttcaccttga aatctcgtct
     2341 tgatcgtatt gatgttttgg gttacaaacg cgacactaaa aactacctta acttgtttga
     2401 tatcgagggc gttgattacg ggttaatcaa agacggtatt tcatttgata aaacgcaaat
     2461 tgctaaaaac ttgaccttgt tcctctaccc agacgattct gacaaaaatg gggaattgct
     2521 ccgtatttac caacagtact ttatggtgtc aaatgcagcg caattaatca ttgatgaagc
     2581 tatcgaacgt ggttcaaacc ttcatgacct tgcagactac gcttacgtgc aaatcaatga
     2641 cacgcatcca tcaatggtca tcccagaatt aattcgtctc ttgactgaaa aacatggctt
     2701 tgactttgat gaagcggtag ctgttgtgaa aaatatggtt ggttacacta accacactat
     2761 tcttgcagaa gcccttgaaa aatggccaac tgcttactta aacgaagtag tgccacactt
     2821 ggtaaccatc attgaaaaat tggatgctct tgttcgttca gaagtgtctg atccagctgt
     2881 tcaaattatt gacgaatcag gtcgtgtgca catggcccat atggatattc attttgcaac
     2941 aagtgtcaat ggggtagcag cactccacac agaaatcttg aaaaacagcg aattaaaagc
     3001 tttctatgac ctttacccag aaaaattcaa caacaaaacc aacgggatta ctttccgtcg
     3061 ttggctagaa tttgctaacc aagacttggc tgattacatt aaagaactta ttggcgatga
     3121 gtacttgact gacgcaacaa aattagaaaa attgatggcc tttgcagatg acaaagctgt
     3181 tcatgctaaa ttggctgaaa tcaaattcaa caacaaatta gctcttaaac gttaccttaa
     3241 agacaataaa ggcattgagc ttgatgaaca ttctattatt gatacccaaa tcaaacgttt
     3301 ccacgagtac aaacgtcaac agatgaatgc tctttacgtg attcacaaat atttggaaat
     3361 taaaaaaggc aaccttccaa aacgtaaaat cactgttatc tttggaggta aagcagcgcc
     3421 tgcttacatc attgctcaag acatcattca cttgatcctt tgcttgtctg aattgattaa
     3481 caatgaccct gaagtaagcc cataccttaa tgtgcatcta gttgaaaatt acaacgtgac
     3541 agtagcagag cacttgattc cagcaactga tatttctgag caaatttcac tagcatccaa
     3601 agaagcttct ggaactggta atatgaaatt catgcttaac ggtgctttaa cacttggtac
     3661 aatggacggt gctaacgtag aaatcgctga gcttgcaggc atggagaata tctacacctt
     3721 tggtaaagat tctgacacca tcatcaacct ttatgcgact gcttcttatg tagcaaaaga
     3781 ttactatgat aaccatcctg ctattaaagc agcagtgaac tttattatca gtccagaatt
     3841 gctagcattt ggcaacgaag aacgtcttga tcgtctttat aaagaattga tttcaaaaga
     3901 ctggttcatg actttgattg accttgaaga gtacattgaa gtgaaagaaa aaatgttagc
     3961 agactatgaa gaccaagatt tatggatgac aaaagtggtc cataacatcg caaaagctgg
     4021 attcttctca tctgaccgta ccattgagca gtataacgaa gatatttggc attcacgata
     4081 aaaaataata attgcaatgt ggggtagcgg taccgtaaca tgcttaaact aaatcataag
     4141 acagtagttt ctgctgtctt atgatttttc ttcagactta gattaattat tacattaagc
     4201 gtaaaacaag tctgtttatc gatctatgaa ggcaattata a
//
LOCUS       DATXIG010000090         4205 bp    DNA     linear   BCT 28-DEC-2023
DEFINITION  TPA_asm: Streptococcus pyogenes strain MGAS11113
            SAMN08555799-rid19682583.denovo.090, whole genome shotgun sequence.
ACCESSION   DATXIG010000090 DATXIG010000000
VERSION     DATXIG010000090.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN08555799
            Sequence Read Archive: SRR6740495
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pyogenes
  ORGANISM  Streptococcus pyogenes
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 4205)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4205)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-OCT-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-OCT-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001918058.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001918058.2
            Genome Coverage       :: 368x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/12/2023 11:23:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 1,806
            CDSs (total)                      :: 1,769
            Genes (coding)                    :: 1,722
            CDSs (with protein)               :: 1,722
            Genes (RNA)                       :: 37
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1, 1 (5S, 23S)
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 47
            CDSs (without protein)            :: 47
            Pseudo Genes (ambiguous residues) :: 0 of 47
            Pseudo Genes (frameshifted)       :: 30 of 47
            Pseudo Genes (incomplete)         :: 20 of 47
            Pseudo Genes (internal stop)      :: 9 of 47
            Pseudo Genes (multiple problems)  :: 11 of 47
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4205
                     /organism="Streptococcus pyogenes"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN08555799-rid19682583.denovo.090"
                     /strain="MGAS11113"
                     /serotype="Emm28"
                     /isolation_source="normally sterile site"
                     /host="Homo sapiens"
                     /db_xref="taxon:1314"
                     /country="Finland"
                     /collection_date="2002"
                     /collected_by="National Public Health Laboratory"
     gene            12..1037
                     /locus_tag="VC122_001627"
     CDS             12..1037
                     /locus_tag="VC122_001627"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002991434.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HEP5641534.1"
                     /translation="MSEKSLGEVLRESRVGKNITLDDIELKTGISSHYLLAMELDQFK
                     IIPEEKFDQFLKEYADIVELDFNTLKRRYRYQVNSKKDSDIKSVTEIVEEKLSKKRLQ
                     EKLLSHQSMGYGQPLEVSKNKETLVIPVKTVGLDSKSPLKTISPVSQFKSDNRLIPKK
                     NVSRMRRNSTDSRSKTSLFPIAILGTVAFAIISVIILGVWQQFEKSQRAKEAEVALLK
                     ASEQSTNGLKNNSNDRKTQVTTEGSGNYLIATVTKSKETVDISVSLTEAQSSWISLTN
                     SEIGEGGITLTQDSPTYTATLPAEITESLLTLGVTKGVSVTIDGQPVDLSPLTSTDLS
                     YITFKIQ"
     gene            1051..1593
                     /gene="pgsA"
                     /locus_tag="VC122_001628"
     CDS             1051..1593
                     /gene="pgsA"
                     /locus_tag="VC122_001628"
                     /EC_number="2.7.8.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000239224.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CDP-diacylglycerol--glycerol-3-phosphate
                     3-phosphatidyltransferase"
                     /protein_id="HEP5641535.1"
                     /translation="MIKKENIPNLLTLVRIAMIPFFLFITSSSNKVGWHIFAAVIFAI
                     ASFTDYLDGYLARKWHVASNFGKFADPLADKMLVMSAFIMLVGLGLVPAWVSAVIICR
                     ELAVTGLRLLLVETGGKVLAAAMPGKIKTATQMLSIILLLCHWIFLGNVLLYIALFFT
                     IYSGYDYFKGASFLFKDTFK"
     gene            1595..2434
                     /locus_tag="VC122_001629"
     CDS             1595..2434
                     /locus_tag="VC122_001629"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002991429.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="energy-coupling factor transporter ATPase"
                     /protein_id="HEP5641536.1"
                     /translation="MSAIIELKKVTFNYHKDQEKPTLDGVSFHVKQGEWLSIIGHNGS
                     GKSTTIRLIDGLLEPESGSIIVDGDLLTITNVWEIRHKIGMVFQNPDNQFVGATVEDD
                     VAFGLENKGIAHEDIKERVNHALELVGMQNFKEKEPARLSGGQKQRVAIAGAVAMKPK
                     IIILDEATSMLDPKGRLELIKTIKNIRDDYQLTVISITHDLDEVALSDRVLVMKDGQV
                     ESTSTPEQLFARGDELLQLGLDIPFTTSVVQMLQEEGYPVDYGYLTEKELENQLCQLI
                     SKM"
     gene            2410..3252
                     /locus_tag="VC122_001630"
     CDS             2410..3252
                     /locus_tag="VC122_001630"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011285331.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="energy-coupling factor transporter ATPase"
                     /protein_id="HEP5641537.1"
                     /translation="MSINLQNVSYTYQAGTPFEGRALFNINLDILDGSYTAFIGHTGS
                     GKSTIMQLLNGLHVPTTGIVSVDKQDITNHSKNKEIKSIRKHVGLVFQFPESQLFEET
                     VLKDVAFGPQNFGVSPEEAEALAREKLALVGISENLFEKNPFELSGGQMRRVAIAGIL
                     AMQPKVLVLDEPTAGLDPKGRKELMTIFKKLHQSGMTIVLVTHLMDDVANYADFVYVL
                     DKGKIILSGKPKTIFQQVSLLEKKQLGVPKVTKLAQRLVDRGIPISSLPITLEELKEV
                     LKHG"
     gene            3245..4045
                     /locus_tag="VC122_001631"
     CDS             3245..4045
                     /locus_tag="VC122_001631"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002991426.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="energy-coupling factor transporter transmembrane
                     protein EcfT"
                     /protein_id="HEP5641538.1"
                     /translation="MDKLILGRYIPGDSLIHRLDPRSKLLAMIIYIVIIFWANNVVTN
                     LLMLTFTLAVVFLSKIKLSFFLNGVKPMIGIILFTTLFQMFFSQGGKVIFSWWFISIT
                     DLGLSQAILIFMRFVLIIFFSTLLTLTTTPLSLSDAVESLLKPLTRFKVPAHEIGLML
                     SLSLRFVPTLMDDTTRIMNAQRARGVDFGEGNLIQKVKSIIPILIPLFASSFKRADAL
                     AIAMEARGYQGGEGRTKYRQLDWQLKDSLAIGIVSLLGLLLFFLKTSL"
BASE COUNT         1341 a          668 c          819 g         1377 t
ORIGIN      
        1 gaggtacctg tatgagtgaa aaaagtcttg gtgaagtgtt aagagaatcg cgtgttggaa
       61 aaaatatcac actagatgat atagaattga agacagggat ttcttcccat tacttgctgg
      121 caatggaatt ggatcagttt aaaattattc cggaagaaaa atttgatcaa tttttaaaag
      181 aatatgctga cattgttgag ttagatttca atactttaaa aagacgatac cgttatcaag
      241 ttaattccaa aaaagacagt gatataaaat ctgtaactga gattgttgaa gagaagttaa
      301 gtaagaaacg attacaagag aagttgttat cacatcagag catggggtat ggtcaaccgt
      361 tagaagtttc taaaaacaaa gaaaccttag ttatccctgt aaagactgtt ggtttagata
      421 gcaaatcgcc actaaaaacg atatcgcctg ttagccaatt taaatcagat aacaggttaa
      481 ttcctaaaaa aaatgtgtca cgtatgaggc gaaatagcac tgacagtcgt tctaaaacgt
      541 cactatttcc catagctata ttaggaacgg ttgcttttgc aattatcagt gttatcattt
      601 taggtgtttg gcaacaattc gaaaagagtc aaagagctaa agaagctgaa gttgctttgc
      661 ttaaagcctc tgagcagtct actaacggtt taaaaaataa ttcaaatgat cgcaaaactc
      721 aagtcacaac tgaggggtcg ggtaattatt taatagcgac tgttactaaa tctaaagaga
      781 ccgttgatat cagtgtgtct ttaactgagg cacaaagtag ttggatttct ctaacaaatt
      841 cagaaattgg tgaaggtggt ataacattga ctcaagactc gccaacttat acagcgactt
      901 tacctgcaga aataacagaa tcattgttga cacttggtgt gacaaaaggt gtatctgtaa
      961 caattgatgg acaacctgtt gatctatcac ctttaacaag tactgactta agttatatca
     1021 cgtttaagat ccaataatta aggtagacta atgataaaaa aagaaaatat tcctaatcta
     1081 ttaactcttg ttcgaatcgc aatgattcct ttctttcttt ttattacttc ctcatctaat
     1141 aaggtggggt ggcatatttt tgcagctgtg atttttgcaa ttgccagttt taccgattat
     1201 ctagatgggt atcttgcgcg taagtggcat gtagccagta attttggaaa gtttgccgat
     1261 cctctagcag ataagatgct tgtcatgagt gcctttatca tgttagttgg acttggctta
     1321 gttcctgcgt gggtgtcagc tgttattatt tgccgagaat tggcagtaac tggtcttcga
     1381 ttactacttg ttgaaaccgg aggaaaggtc cttgcagctg ctatgccggg aaaaatcaaa
     1441 acagcgacac aaatgttgtc tattatttta ttactttgcc attggatatt cctaggaaac
     1501 gtcttactct atattgctct ttttttcact atttattctg gatatgatta ttttaaagga
     1561 gcaagctttc tttttaagga tacgtttaaa taacatgtca gctattattg aacttaaaaa
     1621 agttacattt aattaccata aagaccaaga aaaaccaaca ttagatggcg tatcgtttca
     1681 tgtgaaacaa ggtgagtggc tgtctatcat tggccataac ggatctggta aatcaacgac
     1741 tattcgatta attgatggat tattggaacc agaatcaggg tctattattg ttgatggaga
     1801 tctactaacc ataactaatg tttgggaaat tcgtcataaa attggtatgg tctttcaaaa
     1861 ccctgataat cagtttgttg gagcaactgt tgaagatgat gttgcttttg ggcttgaaaa
     1921 taaaggtatt gcacatgaag atataaaaga gagggttaat catgctttag agttagtcgg
     1981 catgcagaac tttaaagaaa aagaaccagc ccgtttatct ggtggccaaa aacagcgcgt
     2041 agctattgca ggtgcagttg ctatgaagcc taaaattatt attttagatg aagctactag
     2101 tatgcttgac cctaaaggac gattagagtt aatcaaaact ataaaaaaca tccgtgacga
     2161 ctaccagctg actgttattt ccattactca tgacttagat gaagttgctc ttagtgatag
     2221 agttttagtg atgaaagatg gtcaagtgga atcaacatca acaccagaac aattatttgc
     2281 aagaggggat gaactactcc aattaggtct tgatatccct tttacaacat ctgttgtaca
     2341 gatgcttcaa gaagaaggtt atcctgttga ctatggatat ctcacagaaa aggaattaga
     2401 aaatcagtta tgtcaattaa tctccaaaat gtaagttata cttatcaagc agggactcct
     2461 tttgaagggc gtgccctttt taacattaat ttagatattt tagatggttc ttatacggct
     2521 tttatcggac atactggatc cgggaaatcg accattatgc aattgctaaa tggtttacac
     2581 gttccgacaa cgggaattgt cagcgtagat aaacaagata taacgaatca ctctaaaaat
     2641 aaagagatta aatctattcg aaaacatgta ggattagttt ttcaatttcc agaaagtcaa
     2701 ctatttgaag agacagtttt gaaagatgtt gcttttggcc ctcaaaattt tggggtctct
     2761 ccggaggaag cagaagcttt agcgcgtgaa aaactagctc ttgttgggat ttcagaaaat
     2821 ctttttgaaa agaatccatt tgaactctca gggggtcaaa tgcgtcgtgt tgctattgcg
     2881 ggtatccttg ctatgcagcc aaaggtttta gttttagatg aaccgactgc tggtcttgat
     2941 cctaaagggc gtaaagaatt aatgactatt tttaaaaagt tacaccaatc tggcatgact
     3001 attgttttag taacccatct gatggatgat gtggccaatt acgcagattt tgtatatgtt
     3061 cttgacaaag gtaaaattat tttatcagga aaacccaaaa caatttttca acaagtcagt
     3121 ttgcttgaga aaaaacagtt aggagttcct aaggtgacta aacttgctca aaggcttgtt
     3181 gatagaggaa tacctatctc ttctttgcct attaccttag aggagctaaa agaggtacta
     3241 aaacatggat aagctgattc ttggtcgtta tattcccgga gattctctca ttcatcgtct
     3301 tgacccaaga agtaaattat tagcgatgat tatttatatt gtcattatct tttgggctaa
     3361 caatgttgtg acaaatttgt tgatgttgac ttttacttta gcagttgttt ttttgtcaaa
     3421 aatcaagttg tctttctttt taaatggggt taaaccaatg attggtatta tcctcttcac
     3481 gactttattt cagatgtttt tctcacaagg aggaaaagta atttttagtt ggtggttcat
     3541 tagcataact gatttaggat tgagccaagc tattttaatt tttatgcgtt tcgttctaat
     3601 tattttcttt tcaacgctgt taacgttgac gacaacacct ttaagcctat ctgatgcagt
     3661 tgaatcatta ttaaaacctt taacaagatt taaagttcca gctcacgaga tcggactaat
     3721 gctttcttta agtttacgtt ttgtgcctac tttaatggat gataccacgc gtattatgaa
     3781 tgcacaaagg gcccgtggag ttgattttgg agagggaaat ttaattcaaa aagttaaatc
     3841 tattattcct attttaatcc ctcttttcgc ttcaagcttt aaaagagccg acgcattagc
     3901 tattgctatg gaagctagag gttatcaggg aggagaaggg cgaactaaat atcgtcaatt
     3961 agactggcag ctaaaagata gtttagctat tggaatagtt tctttattgg gtttgctact
     4021 ttttttcttg aaaacctctt tgtaatattt tttagcacaa aacttttctt tattagtaag
     4081 aagtgaattg tacctcaaaa gttagataca acatctaatg attgaggtac ttttcttcat
     4141 ttgaggaatt gaaaaccgct atctcaacta tattaatcct cccaataata acaaatcgaa
     4201 cgaaa
//
LOCUS       DATXIG010000091         4122 bp    DNA     linear   BCT 28-DEC-2023
DEFINITION  TPA_asm: Streptococcus pyogenes strain MGAS11113
            SAMN08555799-rid19682583.denovo.091, whole genome shotgun sequence.
ACCESSION   DATXIG010000091 DATXIG010000000
VERSION     DATXIG010000091.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN08555799
            Sequence Read Archive: SRR6740495
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pyogenes
  ORGANISM  Streptococcus pyogenes
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 4122)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4122)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-OCT-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-OCT-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001918058.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001918058.2
            Genome Coverage       :: 368x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/12/2023 11:23:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 1,806
            CDSs (total)                      :: 1,769
            Genes (coding)                    :: 1,722
            CDSs (with protein)               :: 1,722
            Genes (RNA)                       :: 37
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1, 1 (5S, 23S)
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 47
            CDSs (without protein)            :: 47
            Pseudo Genes (ambiguous residues) :: 0 of 47
            Pseudo Genes (frameshifted)       :: 30 of 47
            Pseudo Genes (incomplete)         :: 20 of 47
            Pseudo Genes (internal stop)      :: 9 of 47
            Pseudo Genes (multiple problems)  :: 11 of 47
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4122
                     /organism="Streptococcus pyogenes"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN08555799-rid19682583.denovo.091"
                     /strain="MGAS11113"
                     /serotype="Emm28"
                     /isolation_source="normally sterile site"
                     /host="Homo sapiens"
                     /db_xref="taxon:1314"
                     /country="Finland"
                     /collection_date="2002"
                     /collected_by="National Public Health Laboratory"
     gene            56..151
                     /locus_tag="VC122_001632"
     CDS             56..151
                     /locus_tag="VC122_001632"
                     /inference="COORDINATES: protein motif:HMM:NF038244.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="quorum-sensing system DWW-type pheromone"
                     /protein_id="HEP5641539.1"
                     /translation="MLKKYKYYFIFAALLSFKVVQELSAVDWWRL"
     gene            198..1196
                     /gene="ruvB"
                     /locus_tag="VC122_001633"
     CDS             198..1196
                     /gene="ruvB"
                     /locus_tag="VC122_001633"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_023079556.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Holliday junction branch migration DNA helicase
                     RuvB"
                     /protein_id="HEP5641540.1"
                     /translation="MARILDNDVMGNEEFSDRTLRPQYLHEYIGQDKVKEQFAIFIEA
                     AKRRDESLDHVLLFGPPGLGKTTMAFVIANELGVNLKQTSGPAVEKAGDLVAILNELE
                     PGDILFIDEIHRMPMSVEEVLYSAMEDFYIDIMIGAGDTSRSIHLDLPPFTLIGATTR
                     AGMLSNPLRARFGITGHMEYYQEKDLTEIVERTATIFEIKIDHEAARKLACRSRGTPR
                     IANRLLKRVRDYAQIIGDGIITAQITDRALTMLDVDREGLDYIDQKILRTMIEMYQGG
                     PVGLGTLSVNIAEERNTVEEMYEPYLIQKGFLMRTRTGRVATQKAYRHLGYPYQNT"
     gene            1334..1771
                     /locus_tag="VC122_001634"
     CDS             1334..1771
                     /locus_tag="VC122_001634"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002994668.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="low molecular weight phosphotyrosine protein
                     phosphatase"
                     /protein_id="HEP5641541.1"
                     /translation="MKKVCFVCLGNICRSPMAEFVMKSIVSSDVMMIESRATSDWEHG
                     NPIHSGTQSILKTYQINYDITKCSKQITITDFNTFDYIIGMDSDNVKNLKEMSQHQWD
                     SKIYLFREGGVPDPWYTNDFEETYQLVRKGCQDWLSRLMSKEY"
     gene            1794..2195
                     /locus_tag="VC122_001635"
     CDS             1794..2195
                     /locus_tag="VC122_001635"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002986671.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEP5641542.1"
                     /translation="MIDMQDIVKKWSITRAKLEIVSVIVILVCAISVFSVRISNKTSL
                     TYDKGRMHYTGYVINHKMNGEGKLVYPNGDIYEGTFKNGLFEGKGTFTAKTGWLYNGE
                     FHKGQANGKGVLKAKNNKVYKGIFKQGIFQK"
     gene            2192..3967
                     /locus_tag="VC122_001636"
     CDS             2192..3967
                     /locus_tag="VC122_001636"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010921782.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyltransferase"
                     /protein_id="HEP5641543.1"
                     /translation="MRIKWFSFVRVTGLLLVLLYHFFKNVFPGGFIGVDIFFTFSGYL
                     ITALLIDEYTKKESIDIIGFLKRRFYRIVPPLVLMILLTIPFTFLIKKDFIANIGSQI
                     TAVLGFTTNIYEILTGSSYESQFIPHLFVHTWSLAIEVHFYLLWGVFVWLLARRKETQ
                     KQLRGLLFLISLGIFAISFLSMFIRSFMTSNYSLIYFSSLSHSFPFFLGAMFATITGI
                     NETTVRFQKNVRLWPRQYVLAAMIGAFTLLLVLTVTLDFNHITTYLFGFALASLFASI
                     MIYAARVLHEQTPDVQEPKAITYIADISYGIYLFHWPFYIIFSQLMSHILAVILTVFF
                     SILFATVSYYIVEPLVQGRKPNLLGLEIDCSPYYKWIVGGSLALALLTLGTCMIAPKV
                     GKFEKQLLVSSLQQAQSNMERTHTLAAGDANALSDVGIIGDSVALRSSAAFSKIMPQA
                     QLDAAVSRNFKEAFDLFNNQIKSKSLSKTVVLAVGVNSLDNYSQAVQSFIEALPKGHR
                     LVLVSPYNAKNASQVAEARDYGLKLSKKYKYVTIADWYKVAVEHPDIWYGSDGVHYSE
                     DSQGAELYVSTIQTAVEKSAKKPAK"
BASE COUNT         1272 a          638 c          843 g         1369 t
ORIGIN      
        1 taatagtaag atataattcc taatataaaa aataattatt aaggagtttt tatccatgtt
       61 aaaaaagtat aagtactatt ttatattcgc agctctacta tcttttaaag tagttcaaga
      121 gcttagtgct gttgactggt ggcgattata ataaaagtag gccactgctc tcttttactt
      181 tatgttataa tagtcctatg gctagaattt tagacaatga tgtaatggga aatgaggagt
      241 tttcggatcg gactcttcgc cctcaatatc tacacgaata tattggacaa gataaagtta
      301 aggaacaatt cgctattttt atagaagcag ctaaaagacg tgacgaatct ttagaccatg
      361 ttttattatt tggacctcca ggactgggaa aaacaacaat ggcatttgtg attgccaatg
      421 aattgggagt taatttaaag caaacttcag gtcctgccgt tgagaaagct ggagatttgg
      481 tagctatttt aaatgaatta gaaccagggg atattttgtt tattgatgaa attcaccgca
      541 tgccaatgtc tgttgaggaa gtactatata gtgctatgga agacttctat attgatatta
      601 tgattggcgc tggagatact agtcgtagta ttcatttgga tctgcctcca tttacattga
      661 ttggtgctac tacacgtgca ggcatgttat ctaatcccct cagagctcgt tttggtatca
      721 ctggtcacat ggaatactat caagaaaagg atttgacaga gattgtggag cgtacagcta
      781 ctatttttga aataaaaata gaccatgaag ctgcacggaa attagcttgt cgcagtagag
      841 gaacgccccg cattgctaat cgattactaa aacgagtgcg tgactatgct caaattattg
      901 gtgatggcat cataacggct caaataactg atagagcttt gactatgtta gatgttgacc
      961 gcgaaggtct cgattacatt gatcagaaaa ttttacgaac gatgattgag atgtatcagg
     1021 gaggaccagt tggtttgggg acattatctg tgaatattgc cgaggagcgt aatacagttg
     1081 aagagatgta tgaaccctac ttaattcaaa aaggctttct catgcgaaca cgaacaggtc
     1141 gtgtagcgac tcaaaaggct tatcgtcatc ttggctatcc ctatcaaaac acttaatgat
     1201 ttttcaaatc taagaaaatt aatctgaacc tgctttttgt gttcggattt tttgataagt
     1261 tgacagaaaa ttattagatt gatttctccg atatcagtta gaaatttgat ataataattc
     1321 aaaggagtat ttaatgaaaa aagtatgttt tgtctgtctg ggtaatattt gtcgaagccc
     1381 catggctgag tttgtaatga agtcaatagt atcaagtgat gttatgatga tagaaagtcg
     1441 tgctacttct gattgggagc atggaaaccc tattcactca ggtacacagt ccatattaaa
     1501 aacttaccaa attaattatg atataactaa atgctccaag cagataacta taactgattt
     1561 taacaccttt gactatatta ttggtatgga tagcgataat gtgaaaaatc tcaaagagat
     1621 gtcgcaacat caatgggact ctaaaatcta cctgtttaga gaagggggtg ttcctgatcc
     1681 ttggtacact aacgattttg aagaaaccta ccaattagtt agaaaaggat gccaggattg
     1741 gctttctcgt ttaatgagta aagagtattg atattagaag ttgagtaaaa gaaatgatag
     1801 atatgcaaga tatagtaaaa aaatggagta ttacacgggc gaagttggaa attgtctcgg
     1861 tgattgtcat attggtttgt gcaatttctg tttttagcgt aagaatttcg aataagacaa
     1921 gtttgaccta tgataaaggg cgtatgcact atactggtta cgtgatcaac cataaaatga
     1981 atggagaagg gaagcttgtc taccccaacg gtgatatcta tgagggtact tttaagaatg
     2041 gcctatttga aggaaaagga acttttactg ctaagacggg ctggttatat aacggggagt
     2101 ttcataaagg acaagcaaat ggcaaaggtg tgttaaaagc aaagaataat aaagtctata
     2161 aagggatttt taaacagggg atatttcaaa aatgagaata aaatggtttt cgtttgttag
     2221 ggtaacagga cttttactgg ttttactcta ccactttttt aaaaatgtat ttccaggagg
     2281 ttttattggt gttgatattt tcttcacctt ttcaggatat ctaatcacag ccctcttgat
     2341 tgatgaatac acaaaaaaag agtctatcga tattatcggt tttttaaaaa gacgctttta
     2401 tcgaattgtt cctcctttgg tgttaatgat tttattgacc attcctttta cgtttttaat
     2461 taagaaggat ttcattgcta atataggaag tcagattaca gctgttttag gatttaccac
     2521 taatatttat gaaatattga cgggaagtag ttatgaaagc caatttattc cacatctctt
     2581 tgttcatacg tggagcttag ctattgaagt tcatttttac ttgttgtggg gtgtatttgt
     2641 ttggttatta gctagacgca aggagactca gaaacaactg agaggactac tgtttttgat
     2701 ttctttggga atatttgcta ttagtttctt gagtatgttt ataagatctt ttatgacctc
     2761 aaattattcc ctgatttatt tttcgagtct ttctcacagt tttcccttct ttttaggggc
     2821 gatgtttgcc acaataacag gtattaacga gactactgtt cgatttcaaa aaaatgtacg
     2881 tttatggccg cgccagtatg tccttgctgc tatgattgga gcatttacac ttttgcttgt
     2941 gctaacggtc acattggatt ttaaccatat cacgacctat ctatttggct ttgctttggc
     3001 tagcttattc gcatcaatta tgatttatgc agcgcgtgtt cttcatgagc aaacaccaga
     3061 tgtacaagag cctaaagcta tcacttatat agctgatatt agctatggga tttacctctt
     3121 ccattggcca ttttacatta tttttagcca attaatgtct catattttgg cagttattct
     3181 aacagttttc ttttcaatct tgtttgccac tgtgtcttac tatattgtgg aacctttggt
     3241 ccaagggaga aaaccaaacc tgcttggttt agaaattgac tgttctcctt actataagtg
     3301 gattgtagga gggtctctcg ccttagcatt attaacttta ggaacttgta tgatagcgcc
     3361 taaagttggt aagtttgaga aacaattgtt ggtcagttcc ctacaacagg ctcaaagtaa
     3421 tatggagcgg acacatacac tagctgcagg agacgctaat gctttgagtg acgtaggtat
     3481 tattggtgat tctgtagcat tacgctctag tgcagcattc tcaaaaatta tgccacaagc
     3541 gcaactggat gcagcggtca gtcggaattt taaagaagct ttcgatttat ttaataacca
     3601 aattaagtct aagagtctat ctaagactgt tgtcttagca gtaggtgtta actcgcttga
     3661 caactattct caagcagttc agtcttttat tgaggcattg ccaaaaggac atcgtttagt
     3721 tttggtttct ccatataatg ccaaaaatgc cagtcaggtt gcagaagcta gagactatgg
     3781 tcttaaattg tccaaaaaat acaagtatgt cactattgca gactggtata aagttgctgt
     3841 tgaacatcca gatatttggt atggcagtga cggtgttcat tatagtgaag atagtcaagg
     3901 agcagaacta tatgtctcca caatccagac tgctgttgag aagtcagcta aaaaaccagc
     3961 taaataaagt taagaaatgt ctaagagata agtcagatcc attgtgctct ggctttttct
     4021 tgtgaacaaa tgatgatggt aaaatagtag tactttgagg tttttaaaag aaagcgattt
     4081 cagaaataaa acggcttttt ttagatatat ttctgaaaaa ga
//
LOCUS       DATXIG010000092         3950 bp    DNA     linear   BCT 28-DEC-2023
DEFINITION  TPA_asm: Streptococcus pyogenes strain MGAS11113
            SAMN08555799-rid19682583.denovo.092, whole genome shotgun sequence.
ACCESSION   DATXIG010000092 DATXIG010000000
VERSION     DATXIG010000092.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN08555799
            Sequence Read Archive: SRR6740495
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pyogenes
  ORGANISM  Streptococcus pyogenes
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 3950)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3950)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-OCT-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-OCT-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001918058.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001918058.2
            Genome Coverage       :: 368x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/12/2023 11:23:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 1,806
            CDSs (total)                      :: 1,769
            Genes (coding)                    :: 1,722
            CDSs (with protein)               :: 1,722
            Genes (RNA)                       :: 37
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1, 1 (5S, 23S)
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 47
            CDSs (without protein)            :: 47
            Pseudo Genes (ambiguous residues) :: 0 of 47
            Pseudo Genes (frameshifted)       :: 30 of 47
            Pseudo Genes (incomplete)         :: 20 of 47
            Pseudo Genes (internal stop)      :: 9 of 47
            Pseudo Genes (multiple problems)  :: 11 of 47
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3950
                     /organism="Streptococcus pyogenes"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN08555799-rid19682583.denovo.092"
                     /strain="MGAS11113"
                     /serotype="Emm28"
                     /isolation_source="normally sterile site"
                     /host="Homo sapiens"
                     /db_xref="taxon:1314"
                     /country="Finland"
                     /collection_date="2002"
                     /collected_by="National Public Health Laboratory"
     misc_feature    15..150
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00557"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="ribosomal protein L10 leader region; Derived by
                     automated computational analysis using gene prediction
                     method: cmsearch."
                     /db_xref="RFAM:RF00557"
     gene            179..679
                     /locus_tag="VC122_001637"
     CDS             179..679
                     /locus_tag="VC122_001637"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012658294.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L10"
                     /protein_id="HEP5641544.1"
                     /translation="MSEAIIAKKAEQVELIAEKMKAAASIVVVDSRGLTVDQDTVLRR
                     SLRESGVEFKVIKNSILTRAAEKAGLDELKDVFVGPSAVAFSNEDVIAPAKVINDFTK
                     TADALEIKGGAIEGAVSSKEEIQALATLPNREGMLSMLLSVLQAPVRNVAYAVKAVAE
                     NKEGAA"
     gene            744..1109
                     /gene="rplL"
                     /locus_tag="VC122_001638"
     CDS             744..1109
                     /gene="rplL"
                     /locus_tag="VC122_001638"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018371186.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L7/L12"
                     /protein_id="HEP5641545.1"
                     /translation="MALNIENIIAEIKEASILELNDLVKAIEEEFGVTAAAPVAAAAA
                     GGAEEAAKDSFDVELTSAGDKKVGVIKAVREITGLGLKEAKGLVDGAPANVKEGVAAA
                     EAEEIKAKLEEAGATITLK"
     gene            1368..1967
                     /locus_tag="VC122_001639"
                     /pseudo
     CDS             1368..1967
                     /locus_tag="VC122_001639"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017648301.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing N-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiator protein"
     gene            2142..2467
                     /locus_tag="VC122_001640"
                     /pseudo
     CDS             2142..2467
                     /locus_tag="VC122_001640"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000670032.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DNA cytosine methyltransferase"
     gene            2458..2732
                     /locus_tag="VC122_001641"
                     /pseudo
     CDS             2458..2732
                     /locus_tag="VC122_001641"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001883480.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing N-terminus and C-terminus; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="relaxase/mobilization nuclease domain-containing
                     protein"
     gene            2781..2990
                     /locus_tag="VC122_001642"
     CDS             2781..2990
                     /locus_tag="VC122_001642"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEP5641546.1"
                     /translation="MTDRVMKKPIRGRQLSKRDLYDEEFFRTHFAKIEIESRLEFLLN
                     RANSLEELLLKAKEVNLTIDLKQKM"
     gene            3255..3950
                     /locus_tag="VC122_001643"
                     /pseudo
     CDS             3255..3950
                     /locus_tag="VC122_001643"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002984809.1"
                     /note="frameshifted; incomplete; missing C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="relaxase"
BASE COUNT         1359 a          624 c          845 g         1122 t
ORIGIN      
        1 atataatgat ataggttttg ataaaaaact gacctaagac agcaggggag catgctcata
       61 atattcctgc cgaggcacaa aacgtgatta aagaaaataa cgtatttgta ctttcgtgtc
      121 taggtttagg tgcgattttt ttgtacctag cccaaaaata aaaacggagg taaaactaat
      181 gagtgaagca attattgcta aaaaagctga acaagtcgaa cttattgctg agaaaatgaa
      241 agcagcagca agtatcgttg ttgttgattc acgtggtctt acagttgatc aagatactgt
      301 tcttcgtcgt tcacttcgtg aaagtggcgt tgagttcaaa gttatcaaaa actcaatttt
      361 gactcgtgca gctgaaaaag caggtcttga tgagttgaaa gatgtattcg taggaccatc
      421 tgcagtagca ttttctaacg aagatgttat cgcaccagct aaagttatca acgactttac
      481 taaaactgct gacgcacttg aaatcaaagg tggtgcaatc gaaggcgctg tttcttcaaa
      541 agaagaaatc caagcacttg caacattgcc aaaccgcgaa ggaatgcttt ctatgcttct
      601 ttctgtactt caagctccag tccgcaacgt tgcatacgct gtcaaagctg ttgcagaaaa
      661 caaagaaggc gctgcttaag acgtctcaga gtagcttaag gctacgctta acattttaaa
      721 ttaattattt ggaggaaatc acaatggcat tgaacattga aaacattatt gctgaaatta
      781 aagaagcttc aatccttgag cttaacgatc ttgtaaaagc tatcgaagaa gaatttggtg
      841 taactgcagc tgctcctgta gctgctgcag ctgctggtgg tgctgaagaa gctgctaaag
      901 attcattcga cgttgaattg acatctgctg gcgacaaaaa agttggcgtt atcaaagctg
      961 ttcgtgaaat cacaggtctt ggtcttaaag aagctaaagg tcttgttgat ggagcacctg
     1021 ctaacgttaa agaaggcgtt gctgctgcag aagctgaaga aatcaaagct aaacttgaag
     1081 aagctggagc aacaatcact cttaaataag aagcagatag caatttgaga gcgaaatccc
     1141 gatttatcag tcttttagag cttatagcga tttgaagaaa ctgataaaat gatttggttt
     1201 tttgccaaaa atcctgccaa aaaaatcttt ggcaggattt ttattttcta taaaaattaa
     1261 gctgagtaga gtggttattc tatctatatg tgtctagtaa taattttttt attttcagga
     1321 ggaaaatcat gcaaatgaat gaacagtcat cacgacaaat ggtgattgcc atctatctca
     1381 tttattccaa ctcaaaatga tagtactcct tggctgttcc aagtcgaaat tgttagtgat
     1441 taagaaaacc tacgagacta tggtttgatc gatgaaggcc agcagtctcc cagtgaaaaa
     1501 gggcgtttgg ctaataagat ttatttagga aagttggaac atgatttcac cccagtctta
     1561 agttcagacg gggccagtct caaaaacaga ccagccccgt gttaaattca gcccctagtg
     1621 agactgaaaa gagtgaaact gaaggtagtg aaactaaaga gagtaattta gtcattgagg
     1681 acgaggagga aagaaaggag tgcactagtg tgaaaaagac tgagggtcac tttactcgtc
     1741 aagttgatca tgtcaccaaa tatgacaaag attatatttg gagtttggtt cattcccaat
     1801 tacgtgaagg tggtttatca caagctgcta gtgacttagt catgagctat tttgaagaac
     1861 gctatgctta tgcgctggaa cacatacggt tcgcaagaac agctgaagct attgcggaat
     1921 atgtctttaa tggtgtctta tccgaatgga ccaagcaact aaggtgacaa gaagcgaagt
     1981 gagtaagatg ttttggtggc tattattagg tgtttgggga ctaaggacaa tttggttgat
     2041 tattgaagtt atcactgcac cagaaatgga agattatagt tgagtaagga cgttacataa
     2101 gcagtaaggt ccttttattt tgtcagaaag gaatgagaga tatgcagttt ttagatttgt
     2161 ttgcaggaat tggaggcttt cgattgggtt taattaatca gtgccatgag tgtattggct
     2221 tttgtgagat tgataagttt gcgaggcaat cttacaaagc catctatgag accgaaggag
     2281 aaatagaatt tcatgacatt agacaagtca cagaccaaga ctttagacaa cttagagggc
     2341 aagtggatat catctgcggt ggtttttccc ttgtcaagca ttttcactcg caggcagacg
     2401 actgggattt gagggcacta ggagaactct cttctttgag attactcgag cgaccaaaca
     2461 gataaaaatc atacccataa tcacatccaa tcaacgccat tgaccgtaat tcagataaaa
     2521 aattgatatg gaattatgcc ttggaacgaa acctccgtat gattttagac cgtatttcaa
     2581 aaatgactgg agcaaaaatt atcgagaagc gttattccta ccgtgactat caaaaatata
     2641 ggcagtctag tcataaattt gaattgaaac aacgtcttta ttttttgatg cagcagtcaa
     2701 agttctttga tgatttttta gaaaaagcag agtaattaca tgttcatatt gattttagtc
     2761 agaagcatag cagattcatg atgacggatc gagtaatgaa aaaaccaatt cgaggccgtc
     2821 aactcagtaa acgagattta tatgatgaag aattttttcg tacacatttt gccaagatag
     2881 aaattgaaag tcgattagaa tttttgttga accgtgccaa ttcattagag gagctactat
     2941 taaaagcaaa agaagtgaat ctaaccattg acttaaaaca aaaaatgtaa cttttatcct
     3001 agaagaagat actcaaaaga taagtttagg tcataaaaaa ataagcgata agaaattata
     3061 tgatgtcaat tttttcaaga ttactttaaa aataaggaag tcggtgtttc agatcgatta
     3121 gaaagtctac agaaacagta catgcttttc aagaagaacg agataaagag gaggtatcca
     3181 ctgaagagag taaggaagtc ttcaagaaat acaaggggca acgagatgcc atccatgagt
     3241 ttgaagttga acttgtggaa catcaaattg agaggttaat tgctgaggac atttatatta
     3301 aggtgtcttt tggtgtaaag cagagtggtc ttattttcat tcctaactat caattggata
     3361 ttaggaaaga agaaaatcat aaaaaatata aagtctatat tcgtgagacc gcacaattct
     3421 tcatttacaa caaggaggcg tctaaactta atcgctatat gcgagggcgt gagctcattt
     3481 accagttaac caatgatagc aagagtattc caaaaagaag acgaccaaaa ccattgatac
     3541 acttaagaaa aagattgaag aaataagtct cctcattgag ttagatacgg agaataagcc
     3601 ttatcaagat attaaagatg acattgttaa agatatggct caattagatt tgaccattac
     3661 agagttgcag gaccatattg ctcatctcaa taaggttgcg gaggtcttgc ttaatatgaa
     3721 caacaacgat atagagaacc gccgtttggc cagatatgac tatgccaaga tgaacttgac
     3781 tgcagctata aaaatagaag aagttgagaa agagattgaa acttctcaaa atgaacttaa
     3841 tatatccata gatgagtatg aatatctagt aagaaggttg gaaaagtttg gagagatctt
     3901 gagttatagc aaaattatcg atacttctcg aaatgaaata caatgggagt
//
LOCUS       DATXIG010000093         3939 bp    DNA     linear   BCT 28-DEC-2023
DEFINITION  TPA_asm: Streptococcus pyogenes strain MGAS11113
            SAMN08555799-rid19682583.denovo.093, whole genome shotgun sequence.
ACCESSION   DATXIG010000093 DATXIG010000000
VERSION     DATXIG010000093.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN08555799
            Sequence Read Archive: SRR6740495
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pyogenes
  ORGANISM  Streptococcus pyogenes
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 3939)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3939)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-OCT-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-OCT-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001918058.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001918058.2
            Genome Coverage       :: 368x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/12/2023 11:23:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 1,806
            CDSs (total)                      :: 1,769
            Genes (coding)                    :: 1,722
            CDSs (with protein)               :: 1,722
            Genes (RNA)                       :: 37
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1, 1 (5S, 23S)
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 47
            CDSs (without protein)            :: 47
            Pseudo Genes (ambiguous residues) :: 0 of 47
            Pseudo Genes (frameshifted)       :: 30 of 47
            Pseudo Genes (incomplete)         :: 20 of 47
            Pseudo Genes (internal stop)      :: 9 of 47
            Pseudo Genes (multiple problems)  :: 11 of 47
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3939
                     /organism="Streptococcus pyogenes"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN08555799-rid19682583.denovo.093"
                     /strain="MGAS11113"
                     /serotype="Emm28"
                     /isolation_source="normally sterile site"
                     /host="Homo sapiens"
                     /db_xref="taxon:1314"
                     /country="Finland"
                     /collection_date="2002"
                     /collected_by="National Public Health Laboratory"
     gene            <1..615
                     /locus_tag="VC122_001644"
     CDS             <1..615
                     /locus_tag="VC122_001644"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611423.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS3 family transposase"
                     /protein_id="HEP5641547.1"
                     /translation="VQRLMKVMGLAARIRRKRKYSSYKGEVGKKADNLIKRHFEGSKP
                     YEKCYTDVTELALPEGKLYLLPVLDGYNCEIIDFTLSRSPNLKQVQTMLEKTFPADSY
                     SGTILHSDQGWQYQHQSYHDFLESKGILPSMSRKGNSPDNGMMDSFFGILKSEMFYGL
                     EKSYKTLDKLEEAITDYIFYYNNKRIKAKLKGLSPVQYRTKSFQ"
     gene            complement(915..1787)
                     /locus_tag="VC122_001645"
     CDS             complement(915..1787)
                     /locus_tag="VC122_001645"
                     /EC_number="2.4.2.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000022746.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxylating nicotinate-nucleotide
                     diphosphorylase"
                     /protein_id="HEP5641548.1"
                     /translation="MSIISTDLTPFQIDDTLKAALREDVHSEDYSTNAIFDHHGQAKV
                     SLFAKEAGVLAGLTVFQRVFTLFDAEVTFQNPHQFKDGDRLASGDLVLEIIGSVRSLL
                     TCERVALNFLQHLSGIASMTAAYVEALGDDRIKVFDTRKTTPNLRLFEKYAVRVGGGY
                     NHRFNLSDAIMLKDNHIAAVGSVQKAIAQARAYAPFVKMVEVEVESLAAAEEAAAAGV
                     DIIMLDNMSLEQIEQAITLIAGRSRIECSGNIDMTTISRFRGLAIDYVSSGSLTHSAK
                     SLDFSMKGLTYLDV"
     gene            2011..3321
                     /locus_tag="VC122_001646"
     CDS             2011..3321
                     /locus_tag="VC122_001646"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000581523.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SLC13 family permease"
                     /protein_id="HEP5641549.1"
                     /translation="MIHLIIISAIALAIGIGYRTKINIGLLAIAFSYLIATTLMGLSP
                     KELLHFWPTSLFFTIFSVSLFYNVATTNGTLDVLAQHILYRTRTHPNALYMILYLIAT
                     LLSALGAGFFTTMAICCPLAITLCQKADKHPLIGAQAVNWGASGGANLITSGSGIVFQ
                     GLFKQMGWEEQAFSLGNHIFIVSIIYPLIVLLLLSCYIRYSKGRTNSSLTIDQPPVLS
                     KVQRQTTLLMISSMVLVWLFPLLHLIFPNIAWIATYRQTFDIGFVSILMVCLALRLKL
                     GKQEAILAKVPWAIIIMLCGMSLLMSLAVKSGLVTLIGHLITTTIPHFWLPLFFCVIA
                     GVMSLFSSTLSVVAPTLFPIIATISTQSPHIDIRLLTTATIIGALSTNISPFSSAGSL
                     IQLSLPHIEERSLAFKKQILLGVPISLSLALLTIWILMLLASLS"
     gene            3399..3692
                     /locus_tag="VC122_001647"
     CDS             3399..3692
                     /locus_tag="VC122_001647"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304910.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS3 family transposase"
                     /protein_id="HEP5641550.1"
                     /translation="MPRKTFDKAFKLSAVKLILEEEQSVKMVSSTLEIHPNSLYRWIQ
                     EYEKYGESAFPGHGSALRHAQFETKKLEKEHKLLQEELALLKKFQVFLKPNRK"
     gene            3725..>3939
                     /locus_tag="VC122_001648"
     CDS             3725..>3939
                     /locus_tag="VC122_001648"
                     /inference="COORDINATES: protein motif:HMM:NF024673.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HEP5641551.1"
                     /translation="MNSHHTCQTLQVSRSGFYAYLKRRLSSKHVENEALKEMIKDIFY
                     EHKERYGSVRITQELCRRGIHVNHKRVG"
BASE COUNT         1174 a          881 c          709 g         1175 t
ORIGIN      
        1 gttcaacgtc tcatgaaagt catgggccta gcagcccgca ttcgtcggaa gcgcaagtat
       61 tcttcttaca aaggagaggt tggtaagaag gctgataatc tgattaaacg tcacttcgaa
      121 ggctctaaac cttacgaaaa atgttacact gatgtgacgg agttggcctt acctgaagga
      181 aaactctact tattacctgt tcttgacggc tataactgtg agattattga tttcactctg
      241 tctcggtctc ctaacttgaa acaggttcaa accatgcttg agaagacttt tccagcggat
      301 tcatacagcg gaactattct tcatagtgac caggggtggc aataccaaca ccagtcctac
      361 catgattttt tagagtctaa aggtattctg ccatccatgt cccgcaaagg gaatagcccg
      421 gataacggca tgatggattc cttctttggc attctcaaat ctgaaatgtt ttatggactc
      481 gaaaagtctt ataaaaccct tgacaagctt gaagaagcta ttacagatta cattttttac
      541 tacaacaaca aacgaatcaa agcaaaacta aaaggactta gtcctgtcca atacagaact
      601 aaatcctttc aataattatt tgtccaactt tttggggtca gtacactcct agttaacgac
      661 tttgattgat tttattatga tgaatcgtga tttctgaaaa caaaattttc ttcatggatt
      721 tggcgtggaa cttttacaat aaaaacacta acctctccct atcgtttttt caggtgaatg
      781 cttggaattg gtataataag cggacacgaa tcaggtgaaa acagagaaag tatttgaaga
      841 ggcgtttaaa ctatctgaca taaaactcgt tctagaggaa agacatcctt taaaaagtta
      901 gtttattttg caacttagac atcaaggtag gttaaaccct tcatggaaaa atcgagactc
      961 ttagcactat gggttaaact accactggag acgtaatcaa tcgctaaacc acgaaaacgg
     1021 ctaatagtgg tcatatcaat atttccagaa cattcaatcc gagaacgtcc tgcaattaga
     1081 gtaatggcct gttcaatctg ttccaatgac atattatcca acatgataat atcaacaccc
     1141 gccgccgcag cttcttcggc agcagcaaga ctttccactt ccacctcgac cattttcaca
     1201 aaaggggcat aggcacgcgc ttgagcaatc gccttttgaa cactacctac tgccgcaatg
     1261 tgattgtctt ttagcatgat agcatctgat aaattaaagc gatgattata gccaccgcca
     1321 actctcacgg catatttctc aaaaagacgt aaattaggag tggtttttcg agtatcaaat
     1381 accttaatgc gatcatcgcc taaggcttct acataagcag ctgtcatcga agcaatccct
     1441 gataaatgtt gtaaaaaatt caaggcaacg cgttcacatg ttaagaggct tctcaccgag
     1501 cctatgattt ctaaaaccaa atcgccacta gccaaacgat ctccatcctt aaattgatga
     1561 ggattctgga aggtcacctc ggcatcaaat agggtaaaaa ccctttgaaa aacggttagc
     1621 cccgctaaaa caccagcttc cttggcaaaa agcgacacct tggcttggcc atgatgatca
     1681 aaaatggcat tggtactgta atcttcggaa tgaacatctt ctcgcaaggc tgctttcaat
     1741 gtatcatcta tttgaaaagg ggttaaatca gttgaaatga ttgacatcac taagtcctct
     1801 atttttaata tcacttgtaa gcactagagg caattgctat taacttccgt aggaaatcac
     1861 aaacctgaat acctcgtttt aaattccaac aagtgtcaag acaattgtaa tttttttgtg
     1921 tataatagtc aagtaccaat aggaaacagg tggtcttttg agcgccaaaa ttgccaacct
     1981 atttacatat tctaactaaa ggacaattca atgattcatt taataataat tagcgccatt
     2041 gctctagcca ttggaattgg ttaccgcacc aaaatcaata ttggcctact ggctattgct
     2101 ttttcttacc tcatcgcaac cactctcatg ggattaagcc ccaaagaact tcttcatttt
     2161 tggccaacct cactcttttt taccattttt agcgtctctc tcttttataa cgttgcaaca
     2221 actaacggca ctcttgatgt tttggctcaa cacattctct accgcacacg cacccaccct
     2281 aacgccctct acatgatttt atacctgata gcaacccttt tgtctgcttt aggtgctgga
     2341 tttttcacta ctatggccat ttgctgtcct ctagcgatta ccctctgtca aaaagcggac
     2401 aaacaccctt tgattggagc tcaagccgtc aattggggag cttcaggagg ggctaatttg
     2461 ataaccagtg gctcaggcat tgtctttcaa ggcctgttta agcaaatggg atgggaagag
     2521 caagcttttt cacttggcaa tcatatcttt atcgtcagca ttatctaccc actcatcgtc
     2581 ttgcttttat tatcttgtta catccgctac agtaagggaa gaacaaactc atccttgact
     2641 attgaccaac caccagtcct cagcaaggtc caaagacaaa ccaccttgct catgatcagc
     2701 agcatggttt tggtttggct ttttcccttg ttgcatctca ttttcccaaa catcgcatgg
     2761 atagctactt atcgacaaac atttgatatt ggctttgtct caatactcat ggtctgcctc
     2821 gccttaaggc tcaaattagg gaagcaagaa gccattttgg ctaaggttcc ctgggctata
     2881 attatcatgc tctgcggcat gagcttacta atgtctctag cggtcaagtc aggtttagtt
     2941 acactgatag ggcacctcat aacaacaacc attcctcatt tctggttacc tctgttcttt
     3001 tgtgtgatag ccggagtgat gtcactcttt agttccaccc tatccgttgt agccccaact
     3061 ctctttccca tcattgctac catcagtact caaagtcctc acattgatat tcgcttactc
     3121 acaacagcca ctattattgg cgctctatct actaatatct ctcctttttc atcagctggt
     3181 tccctcattc aactctccct tccccatata gaggaacgca gtcttgcttt caaaaaacaa
     3241 attcttctgg gtgtgcccat cagcttaagt ttggcactct tgaccatctg gattctcatg
     3301 ctgctagctt ccttaagtta gatttattgt ctaacatttg gttggacttg gtatactaaa
     3361 gtagacacaa aattaagtgg aaacgaggaa tagtcattat gccaagaaaa acctttgata
     3421 aagccttcaa actctctgct gtaaaactca tccttgagga agagcagtct gtaaaaatgg
     3481 ttagttcaac tttagaaatt catcccaata gtctttatcg atggattcaa gaatatgaaa
     3541 aatatggaga aagtgcgttc ccaggacatg ggagcgcact tcgtcatgct caatttgaga
     3601 caaaaaaact tgaaaaagag cacaaactgc tacaggagga attagctctt ctaaaaaagt
     3661 tccaggtctt tttgaagcca aaccggaaat aaaatttcag tttctgaagg aaaatagtgt
     3721 tagcttgaat agtcatcaca cctgtcaaac tcttcaagtc tcccgttcag gtttctatgc
     3781 ttatttaaaa cgtcgtctat cttcaaagca tgttgagaat gaagctttga aggaaatgat
     3841 taaagacatt ttctatgaac ataaagagcg ttacggaagt gtacgaatta cccaagaact
     3901 ttgtagacgt ggcatccatg tcaatcataa acgtgttgg
//
LOCUS       DATXIG010000094         3616 bp    DNA     linear   BCT 28-DEC-2023
DEFINITION  TPA_asm: Streptococcus pyogenes strain MGAS11113
            SAMN08555799-rid19682583.denovo.094, whole genome shotgun sequence.
ACCESSION   DATXIG010000094 DATXIG010000000
VERSION     DATXIG010000094.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN08555799
            Sequence Read Archive: SRR6740495
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pyogenes
  ORGANISM  Streptococcus pyogenes
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 3616)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3616)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-OCT-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-OCT-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001918058.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001918058.2
            Genome Coverage       :: 368x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/12/2023 11:23:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 1,806
            CDSs (total)                      :: 1,769
            Genes (coding)                    :: 1,722
            CDSs (with protein)               :: 1,722
            Genes (RNA)                       :: 37
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1, 1 (5S, 23S)
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 47
            CDSs (without protein)            :: 47
            Pseudo Genes (ambiguous residues) :: 0 of 47
            Pseudo Genes (frameshifted)       :: 30 of 47
            Pseudo Genes (incomplete)         :: 20 of 47
            Pseudo Genes (internal stop)      :: 9 of 47
            Pseudo Genes (multiple problems)  :: 11 of 47
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3616
                     /organism="Streptococcus pyogenes"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN08555799-rid19682583.denovo.094"
                     /strain="MGAS11113"
                     /serotype="Emm28"
                     /isolation_source="normally sterile site"
                     /host="Homo sapiens"
                     /db_xref="taxon:1314"
                     /country="Finland"
                     /collection_date="2002"
                     /collected_by="National Public Health Laboratory"
     gene            73..351
                     /locus_tag="VC122_001649"
     CDS             73..351
                     /locus_tag="VC122_001649"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013643575.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEP5641552.1"
                     /translation="MYDEGTGFKPIHFYGIAICEGTREFYRPTYPFVRSEEDLDNLKD
                     FINLYETDLLTFYTHGHNYDFGCFIYGIGNDGKDKFRDRWFKEGVIFY"
     gene            444..2684
                     /locus_tag="VC122_001650"
     CDS             444..2684
                     /locus_tag="VC122_001650"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018366738.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="C39 family peptidase"
                     /protein_id="HEP5641553.1"
                     /translation="MKQTKVLTGIAVAALSTGTGLVQADDLSNTRPAKAAEVVTHTEV
                     TENQVADAKTKADEATKAVKDQEATVKAIEAEKSQAQQSLVEATTAVNDTEKLASEAT
                     PDGLTKAQEETEASQSAVTDAQAQLEAAQEAESKAQTAVDHQKETVAKASQAVNQAKA
                     ALTTSNQAADVSAAEEKVLEAQEAVTKAETQVSQAQEADAKHESDVRHAKEELDLKSQ
                     KLTEKQTTLEQVMAAIEAERLTKPVENGTYFNQRDNEWQKAYGGKTFAATGCVPSALA
                     MVFSELAKREVTPTEIADYLWHNTDEFNKHYGGTSGKGLVAATNHYGFASTHLDSQST
                     IIAALQAGHHVLAAVQNNKFSPWGSQYSHEIVLRGYSNGNTYVYDPYNRANIGWYPIA
                     NLWNEQSRDAIDTAEVGVPFFKITTQKMAQLEMAKAVANEGLDEAKQAVKQAEANVTA
                     LEQVISQTSQAKEQLAAAKANLVTAKDMLDKAKQTAKLTEQDLETKTANLKQAQEHLT
                     LKQDDLAKAQTELAAQEKSLEQLTQVLSLAQENLGRATKAVEQTKADLTQKQAYVTNL
                     KQVTKLRQESQAKVNQAKAVLSEASARLEQELETLKTLQAKEANLTAQYQAIFKAYQA
                     VLAVKHHSQLAESKAKLEAKGEEAVPVVNEVGHVTDYVSGPTQLADAVAVRTEETSSK
                     TKNTSPVSVKLMAKQGHQKALETIVTSQSSLPQTGEQVVSILSLVGLNFLGLSSFLGL
                     RKQEEK"
     gene            2854..>3616
                     /locus_tag="VC122_001651"
     CDS             2854..>3616
                     /locus_tag="VC122_001651"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011285012.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell surface protein"
                     /protein_id="HEP5641554.1"
                     /translation="MKQMETKGYGYFRKTKAYGLVCGITLAGALTLGTTSVSADDVTT
                     LNPATNLTTLQTPPTADQTQLAHQAGQQSGELVSEVSNTEWDNAVTTAQKAGVTVKQS
                     EKVTHDSLSSAQADLEKQTQAVTEATTKQEANTTAINQAVSENKAIDQANRDEKARVD
                     ALNTKGEADTKAKNETGQAQVDAQNKQAQAAADAMNAKLKADYEAKLTEIKQIESENE
                     AIRQRNQQASQATHQANQAAQAAYQEKLAEIERIKA"
BASE COUNT         1332 a          647 c          783 g          854 t
ORIGIN      
        1 tctaggaaag gagtacaaga atacgatgta acaaatgtat ctgaaagaag tataaagata
       61 attaagaaag ctatgtatga tgaaggaaca ggttttaagc caattcattt ttatggaata
      121 gctatctgcg aagggactag agaattttat agacctactt atccatttgt tagaagtgaa
      181 gaagatttag ataacttaaa agattttatt aacttatatg agacagattt gttaactttt
      241 tatacacatg gtcataatta tgactttggc tgttttattt atggtatagg aaatgacgga
      301 aaagataagt tccgtgatag atggtttaaa gaaggagtca ttttctattg aattttaaaa
      361 taattaaaaa aaagatgacc caaggtaaca ttaaagtagt ataataatct taataaagga
      421 agttatagga agggcaacta cttatgaaac aaacaaaagt attaacagga atcgcagtcg
      481 cagctttatc tacaggtaca ggtcttgtgc aggcagatga tttatcaaac acaagaccag
      541 ctaaagcagc tgaagttgtg acacatacgg aagtgacaga aaatcaagtg gcggacgcta
      601 aaacaaaagc tgatgaggca acgaaagccg ttaaggatca ggaagctacg gttaaggcta
      661 ttgaggcaga aaaatctcaa gcacaacaat ctttagttga ggcgacaaca gcagttaatg
      721 ataccgaaaa gttagctagt gaagcgacac cagatggtct aacaaaagcc caagaagaga
      781 ctgaggctag tcaaagcgct gttactgatg cacaagcaca acttgaagca gctcaagagg
      841 ctgaatcaaa agcccaaaca gcagttgacc atcagaaaga aacagtcgcc aaagcgtctc
      901 aagctgttaa tcaagccaaa gcagctctca cgacaagtaa tcaagccgct gatgttagtg
      961 cagcagaaga aaaagtgtta gaggctcaag aagcggttac gaaagcagaa acacaagtta
     1021 gtcaggctca agaagcagat gctaaacatg agtctgatgt gagacatgcc aaagaagagc
     1081 ttgaccttaa gtctcaaaaa ttaacagaaa aacaaacaac tctagaacaa gtcatggctg
     1141 cgattgaagc agagcgtctg actaagccag ttgaaaatgg cacttacttt aaccaaagag
     1201 ataacgagtg gcaaaaagcc tatggtggga agacctttgc ggcgacagga tgtgtgccta
     1261 gtgctttggc tatggttttt tcagagctgg ctaaaagaga ggtgacaccg actgagattg
     1321 ctgattactt atggcataac acagatgagt ttaataaaca ttatggtggt acaagtggta
     1381 aaggacttgt agctgctact aatcattatg gctttgcttc aacacattta gatagtcaat
     1441 caactattat tgcagcttta caagcaggtc atcatgtttt ggctgcagta cagaataata
     1501 aatttagtcc ttggggatca caatatagtc atgaaattgt cttgcgtggt tactcaaatg
     1561 gcaatactta tgtttacgat ccttataatc gagctaacat tggttggtac cctattgcta
     1621 atttatggaa tgaacaaagt cgtgatgcta ttgatacagc agaggttgga gtacctttct
     1681 ttaaaataac aactcaaaaa atggcacaac tcgaaatggc aaaagcagta gctaatgaag
     1741 gcttagatga agctaagcaa gctgttaaac aagcggaagc aaatgtgaca gccttagaac
     1801 aagttatttc tcaaacctca caagccaaag aacaattagc tgcagctaag gctaacttag
     1861 tcacagccaa agatatgctg gataaggcta aacaaacggc taaattaaca gaacaagact
     1921 tagaaacgaa aacagctaat cttaagcaag ctcaagaaca tctaaccctt aaacaagatg
     1981 acctagctaa agctcaaacg gagctagcag ctcaagagaa aagcttggaa cagttaactc
     2041 aagtgctttc attagctcaa gaaaatcttg gccgtgctac aaaagcagtt gaacagacta
     2101 aggctgatct tacccaaaaa caggcttatg tgacgaatct taagcaagtg acaaaactga
     2161 gacaagaaag tcaagccaaa gtaaaccaag ctaaggcggt tctcagtgaa gcaagtgctc
     2221 gtttagaaca agaacttgag accttaaaaa ccttacaagc caaagaggct aacctaacag
     2281 cacagtatca agctatcttt aaagcctatc aagctgtttt agctgtgaaa caccactcac
     2341 aattggctga aagcaaagct aagcttgaag ctaagggaga agaagctgtt cctgtggtga
     2401 atgaagtggg acacgtaacg gattatgtgt ctgggccaac acagttggct gatgctgtag
     2461 cagtgagaac agaagaaaca tcatcaaaaa caaagaacac ttctcctgtt agtgtgaaat
     2521 tgatggccaa gcaaggtcat caaaaggctc ttgaaactat cgttacttca caaagtagcc
     2581 ttcctcagac aggtgagcaa gttgtctcta ttttgagctt agtaggtttg aattttttag
     2641 gcttaagtag ttttttgggg cttaggaagc aagaggaaaa gtaataaaat atagaacaag
     2701 aggtcattct ttagagaatg gctttttgat tggagttatg atgaaaatag tttttaattg
     2761 gaaggcaaga ttaccttcct tttcttttta gtaaaacata taagacctgg gatcgtcaca
     2821 aaaagcaacc tagaagagag atgagaaaat aacatgaaac aaatggaaac taagggttat
     2881 ggttatttta gaaaaacaaa ggcctatggt cttgtttgtg gtattacttt agcgggagcg
     2941 ttgacgttgg gtacaacaag tgtttctgcg gatgacgtaa ctactttaaa cccagcgact
     3001 aacttaacaa cgctacaaac acctccaaca gctgatcaaa cacagctagc tcatcaagct
     3061 ggccagcagt caggggagtt ggtatcagag gtgagcaata ccgaatggga taatgctgtc
     3121 acaacagctc aaaaagcagg tgttactgtc aaacagtctg agaaggtcac acatgatagc
     3181 ttatcatctg cgcaagccga tttagagaaa caaactcaag ctgtcacaga agcgacaact
     3241 aaacaagaag ccaacacaac agctattaat caagcagttt cggaaaataa agcgattgat
     3301 caagctaatc gtgatgaaaa agctagagtt gacgctctta atactaaagg ggaagctgat
     3361 accaaagcta aaaatgaaac tggtcaagcc caagtagatg cacaaaataa acaagcccaa
     3421 gcagcagctg atgccatgaa tgccaaatta aaggcagact atgaggccaa gttaacagaa
     3481 atcaagcaga ttgaatcaga aaatgaagct atccgtcagc gcaaccaaca agctagtcaa
     3541 gcgactcatc aagccaatca agcagcccaa gctgcctatc aagaaaagct agcagagatt
     3601 gagcgcatca aagccg
//
LOCUS       DATXIG010000095         3518 bp    DNA     linear   BCT 28-DEC-2023
DEFINITION  TPA_asm: Streptococcus pyogenes strain MGAS11113
            SAMN08555799-rid19682583.denovo.095, whole genome shotgun sequence.
ACCESSION   DATXIG010000095 DATXIG010000000
VERSION     DATXIG010000095.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN08555799
            Sequence Read Archive: SRR6740495
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pyogenes
  ORGANISM  Streptococcus pyogenes
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 3518)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3518)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-OCT-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-OCT-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001918058.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001918058.2
            Genome Coverage       :: 368x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/12/2023 11:23:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 1,806
            CDSs (total)                      :: 1,769
            Genes (coding)                    :: 1,722
            CDSs (with protein)               :: 1,722
            Genes (RNA)                       :: 37
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1, 1 (5S, 23S)
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 47
            CDSs (without protein)            :: 47
            Pseudo Genes (ambiguous residues) :: 0 of 47
            Pseudo Genes (frameshifted)       :: 30 of 47
            Pseudo Genes (incomplete)         :: 20 of 47
            Pseudo Genes (internal stop)      :: 9 of 47
            Pseudo Genes (multiple problems)  :: 11 of 47
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3518
                     /organism="Streptococcus pyogenes"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN08555799-rid19682583.denovo.095"
                     /strain="MGAS11113"
                     /serotype="Emm28"
                     /isolation_source="normally sterile site"
                     /host="Homo sapiens"
                     /db_xref="taxon:1314"
                     /country="Finland"
                     /collection_date="2002"
                     /collected_by="National Public Health Laboratory"
     gene            1123..>3518
                     /locus_tag="VC122_001652"
     CDS             1123..>3518
                     /locus_tag="VC122_001652"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002990440.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1542 domain-containing protein"
                     /protein_id="HEP5641555.1"
                     /translation="MLKKIEVKEGQKNPISITLSEDQAKSLKNKEKLKVSIKQKQSKK
                     TSKDFFFEVGIDPKVKAKQQEKLLELDKVKKQIEDSINGDAWLPEKSEGEKPVQNTNK
                     ELQLQELNKKYQMAKEAIESATTLDDVETQFDKYTKVGDKDKYPDSLRNQYTRGDKDK
                     EIEKAKKSLGDLSDKVNGKIEEDKWLSDEVKKKQQQELEARKQKVNDSLKGSDSLKSL
                     RETVEKASSKNQKKPESFEDVYVPGNEETEKTKVRDILQKTYQKTEQNIETDPWLSPE
                     QKKAQKENAKARLDAGLKAVETTESLDKLKEVESDFLDKEKNPDSIPNQHKAGTPEVA
                     RKTFLDNFEKEAKKEIESIDKDDTLTANAKQVAKDKVAQQLQEATAKVEKAQSFDDLK
                     NIQDKFNSELPHTPGKPLKDQQSDAIAELEKKQQEIEKAIEGDKTLPRDEKEKQIADS
                     KERLKSDTQKVKDAKNADAIKKAFEEGKVDIPQAHIPGDLNKDKEKLLAELKQKADDT
                     EKAIDSDKTLTAKEKEEQKAKTKEELQKATEAVGAIDNREELDKKVPELKKAIEDTHV
                     KGNLEGIKNKAIEDLKKAHTETVAKINGDDTLDKATKEAQVKEADKALAAGKEAITKA
                     DDADKVSTAVTEHTPKIKAAHKTGDLKKAQVDANTALDKAAEKEREEINNDITLTAKD
                     KEQQLKEVETALTKAKDNVKAAKTADAINDARDKGVATIDAVHKAGQDLGARKSGQVA
                     KLEEAAKATKDKISADPTLTSKEKEEQSKAVNAELKKAIEAVNAADTADKVDDALGKG
                     V"
BASE COUNT         1475 a          538 c          692 g          813 t
ORIGIN      
        1 aaattaataa ttcatttaaa ttctaatcag ctgtaacgtt ccggggcgtg ttaaactgtc
       61 tgaacataga aaatacaggt ttctacggtg actaaaataa gctaaggagg attccttaaa
      121 ttctgtgata ggaacgtaaa ctctctacaa gagtagagca cgtcatcagt cagtctgggt
      181 gtctactggc tgacaacgtc aaataagata ggcatgaaaa aataaggggg aatacagaat
      241 gtttttaaaa caccaagatg tgaaacaaaa aaattggaga atgcgtaagg tcaaaaaagt
      301 ctttgttagt tcatgtatgc ttttaacagt gggcctcgga gttgccgtac tactgcattc
      361 agccaatcta atggcgtgat ggtcgtaaag gctgcggaag ctgaagaact gccagacaat
      421 ttaattgatt ttaaaggaac ttgggaagta tctgccgatg gctcttctgg tcgcttctat
      481 tcagacggtg ctacaggtca atataagttt caccttattc cagcttcaga tgttaaaaat
      541 ccgggatggc atgaacataa taaagttaaa gatagctatg tcaaaataac caaagagtct
      601 attgcagcta gatatacaaa taaaacgaaa cctccatatt ctgtagcgtt taaggtcaac
      661 actaaatctt taataaaaga tcatgactat aagataactt ttgagcaagg ttcaattgcg
      721 tcgggaataa cagttgatta tcgtataggc agtgctttta ataaaactaa agatgattct
      781 ttcaacattt cagatgagag taaatatgct tctaaggtaa caatagaagg tgaggaaaga
      841 ggttttataa ataggaaacc aggaactaaa acaatatctt ttagagctgt agaaaaagga
      901 actaaatctc ttgtattgtt atctaaagtt aatgaaaaaa cccaagtaga tcttgatgtc
      961 gagtttaaaa acttcaaaat tattgatgtt accaacccaa gccaattaga taaaggagtt
     1021 gcttacgttg gaaacaaaaa tgtagaattg acattaaaat cagacgatgg aagaacaaac
     1081 ttcgaaggtg atgagataag cctttttaag cctaatggag atttgttaaa gaaaattgaa
     1141 gttaaagaag gacaaaaaaa tcctatatca ataactttat ctgaagatca agccaaatct
     1201 ttaaaaaaca aagaaaagtt aaaagtaagc attaaacaaa agcaatcaaa aaagacttct
     1261 aaagactttt tctttgaggt aggaattgat cctaaagtta aagcaaagca acaagaaaaa
     1321 ctcttagaac tagacaaagt aaaaaaacaa attgaagact caatcaatgg tgatgcatgg
     1381 ctaccagaaa aatcggaagg cgaaaaacca gtacaaaata ctaataaaga attacagtta
     1441 caagaactga acaagaagta tcagatggcc aaagaggcaa ttgaaagtgc tacaactctt
     1501 gacgatgttg aaactcagtt tgataaatac acaaaagttg gagataaaga taaatacccg
     1561 gattctttac gtaaccaata tactcgagga gataaagata aagaaataga aaaagctaaa
     1621 aaatcactgg gtgatttaag cgataaagtg aatggaaaga ttgaagaaga taagtggcta
     1681 tctgacgaag ttaaaaaaaa acaacagcaa gaattagaag cgcgtaaaca aaaagtaaac
     1741 gactctctta aaggatctga tagtttaaaa tcattgagag aaacagtgga aaaagcttct
     1801 tctaaaaatc agaaaaaacc agaatctttt gaagatgttt atgttcctgg aaatgaagaa
     1861 actgaaaaaa caaaagttcg tgatatactt caaaaaactt atcaaaaaac agagcaaaat
     1921 attgaaacag atccatggtt gagtccggaa cagaaaaaag ctcaaaaaga aaacgctaaa
     1981 gcaagattag atgcaggttt gaaagctgtc gaaactacag aaagcttaga taaacttaaa
     2041 gaagttgaga gtgacttcct tgataaagaa aaaaatccag attcaattcc aaatcaacat
     2101 aaagctggaa ctcctgaagt tgctcgcaaa actttcttag ataactttga aaaagaagcc
     2161 aaaaaagaga ttgaatctat tgataaagat gataccttaa cggcaaatgc taaacaagta
     2221 gctaaagata aagtggcaca acagctacaa gaagctacag ctaaagtaga gaaagcacag
     2281 tcgtttgatg atttgaagaa cattcaagat aagttcaatt ctgaattacc acacacacca
     2341 ggtaagccgc ttaaagatca acaatctgat gctattgcag aattagagaa gaagcagcaa
     2401 gaaattgaaa aagctattga gggtgataaa acattaccaa gagacgaaaa agagaaacaa
     2461 attgctgact ctaaggaacg cttaaaatct gacacgcaaa aagttaaaga tgctaaaaat
     2521 gctgatgcta ttaaaaaagc atttgaagaa gggaaagtgg atattcctca agcacatatc
     2581 ccaggtgatt tgaacaagga taaagaaaaa cttcttgcag agttgaagca aaaagcagat
     2641 gatactgaaa aagcgattga ttccgataaa actttgacag cgaaagaaaa agaagagcaa
     2701 aaagcgaaaa caaaagaaga acttcaaaaa gcaacagaag ctgttggagc aattgacaat
     2761 cgtgaagaac tagataaaaa agtcccagaa cttaagaaag ctattgaaga cactcacgtt
     2821 aaaggtaatc ttgaaggtat taagaataag gctattgaag atcttaaaaa agctcatact
     2881 gaaacagttg ctaaaataaa tggtgatgat acccttgaca aagctactaa agaagctcaa
     2941 gtgaaagaag ctgacaaagc tttggcagca ggtaaagaag ctatcactaa agcagatgat
     3001 gctgataaag taagtacagc tgttacagag cacacaccaa aaattaaagc agcacataaa
     3061 actggtgatc ttaaaaaagc tcaagtagat gctaacacag ctcttgacaa agcagctgaa
     3121 aaagaacgtg aagaaatcaa taatgatatc acattaacgg caaaagacaa agaacaacaa
     3181 ctgaaagaag ttgagacagc tcttactaaa gctaaagata acgtgaaagc tgctaagaca
     3241 gcagacgcta tcaatgacgc acgtgataaa ggcgtagcaa caattgatgc cgttcataaa
     3301 gcaggtcaag acttaggtgc tcgtaagtca ggtcaagtcg ctaaacttga agaagcagct
     3361 aaagcaacga aagacaagat ttcagctgat ccaactttga caagcaaaga aaaagaagag
     3421 caatctaaag ctgttaacgc tgaacttaag aaagcgatag aagctgttaa cgcagctgac
     3481 acagctgaca aggttgacga tgctcttggt aaaggtgt
//
LOCUS       DATXIG010000096         3272 bp    DNA     linear   BCT 28-DEC-2023
DEFINITION  TPA_asm: Streptococcus pyogenes strain MGAS11113
            SAMN08555799-rid19682583.denovo.096, whole genome shotgun sequence.
ACCESSION   DATXIG010000096 DATXIG010000000
VERSION     DATXIG010000096.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN08555799
            Sequence Read Archive: SRR6740495
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pyogenes
  ORGANISM  Streptococcus pyogenes
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 3272)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3272)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-OCT-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-OCT-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001918058.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001918058.2
            Genome Coverage       :: 368x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/12/2023 11:23:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 1,806
            CDSs (total)                      :: 1,769
            Genes (coding)                    :: 1,722
            CDSs (with protein)               :: 1,722
            Genes (RNA)                       :: 37
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1, 1 (5S, 23S)
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 47
            CDSs (without protein)            :: 47
            Pseudo Genes (ambiguous residues) :: 0 of 47
            Pseudo Genes (frameshifted)       :: 30 of 47
            Pseudo Genes (incomplete)         :: 20 of 47
            Pseudo Genes (internal stop)      :: 9 of 47
            Pseudo Genes (multiple problems)  :: 11 of 47
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3272
                     /organism="Streptococcus pyogenes"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN08555799-rid19682583.denovo.096"
                     /strain="MGAS11113"
                     /serotype="Emm28"
                     /isolation_source="normally sterile site"
                     /host="Homo sapiens"
                     /db_xref="taxon:1314"
                     /country="Finland"
                     /collection_date="2002"
                     /collected_by="National Public Health Laboratory"
     gene            32..901
                     /locus_tag="VC122_001653"
     CDS             32..901
                     /locus_tag="VC122_001653"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002984334.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional DNA primase/polymerase"
                     /protein_id="HEP5641556.1"
                     /translation="MYHTTALSFLKKGYQVIPLRKDTGTPMIKFKDIQITEEVIKNTN
                     WFNCDYALLMRGIWCIDIDTHDMDEKLAKELYIMIKKMGIDLLSVLSTDKYDNGLDGY
                     SSIIRHEYKNELISNFKNTFAELTASGGMHILFKKRDGINYTQKIGVMPGVDIKANDN
                     NFVKIFPSDGREVLQAVKTLPYYDGKFEEEAFKPKQEGITTYFGGSVTYTSNGSHEGR
                     EAYERVATGTSYNRNNDLFKGACWAFENGIDIDDLTSIIGTVKGRDVFTREEFELTIE
                     SAKRKVSYVTIRT"
     gene            855..1205
                     /locus_tag="VC122_001654"
     CDS             855..1205
                     /locus_tag="VC122_001654"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002984338.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VRR-NUC domain-containing protein"
                     /protein_id="HEP5641557.1"
                     /translation="MNQRNEKSATSLSEHDIQNLIRMELSQAGHMVFRANVGKVKTAD
                     GRFFDTGLPKGFCDLFGFKPNGQIFFIEVKNETGRIRPEQKKFMEVMASRGALVGVAR
                     SVEDALNIVNDTSR"
     gene            1189..1545
                     /locus_tag="VC122_001655"
     CDS             1189..1545
                     /locus_tag="VC122_001655"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002984340.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEP5641558.1"
                     /translation="MTLVDDFYKQMEPSIKAFLDDNITIADKEEADRVYRSVKYYKKL
                     NRLPPPDVLEWFQRIYTTKEMIMLIKQSYRLKQKKTDEDDKIYEKWMFKNYGDVKLVK
                     KIKRTDALERARRMGL"
     gene            1787..2023
                     /locus_tag="VC122_001656"
     CDS             1787..2023
                     /locus_tag="VC122_001656"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002995955.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3310 domain-containing protein"
                     /protein_id="HEP5641559.1"
                     /translation="MIEELVHKPKHYNHGEIDLIESWYKTYPWDQFVAIMESHIDKYI
                     KRHRYKGNATQDLEKALEYTKRLIEYWLIESVEE"
     gene            2020..2190
                     /locus_tag="VC122_001657"
     CDS             2020..2190
                     /locus_tag="VC122_001657"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002995952.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEP5641560.1"
                     /translation="MKRYIEVKDEWKSATDHLNDFIDKNKYAKVTVVGYQVVQLSPYG
                     RDLTYILAEVEE"
     gene            2187..2471
                     /locus_tag="VC122_001658"
     CDS             2187..2471
                     /locus_tag="VC122_001658"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015984796.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEP5641561.1"
                     /translation="MTIDEALQNLRDNFNKIMNVLKNDWKALLFLAIAIFGMMVTVSY
                     FSYRDARQYYESQITGLRTQLSRTQKQLKRASEDRARQTKRIAEFTHNGG"
     gene            2473..3105
                     /locus_tag="VC122_001659"
     CDS             2473..3105
                     /locus_tag="VC122_001659"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011017868.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SAM-dependent DNA methyltransferase"
                     /protein_id="HEP5641562.1"
                     /translation="MIKIDEIHRILGIDEVYKAPKRLTDILFDKDSREDIFRQFLKYE
                     TDVSYDWFMQYFEEEQADRKNKKQDFTPKSVSTLLSKIISGNQYYEVAVGTGGILIQA
                     WQEQRLNDSPFTYRPSKYWYHVEELSDKAVPFLLFNMSIRGINGVVVHGDSLTRQVKN
                     IYFLQNTKDDMLSFSDINVMPRTQDIEQEFNVKEWIGDAIVHVESKLNAR"
     gene            3110..>3272
                     /locus_tag="VC122_001660"
     CDS             3110..>3272
                     /locus_tag="VC122_001660"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011888686.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1642 domain-containing protein"
                     /protein_id="HEP5641563.1"
                     /translation="MNIEEAIKKIRELEAFNYFDFKGNLIRRIDVLGIIDQLKIIVQQ
                     CVADWYEKHK"
BASE COUNT         1223 a          475 c          702 g          872 t
ORIGIN      
        1 ttacgtagta aaaaaggggg tgcgttacct tatgtaccac acaacagcac tttcattctt
       61 aaaaaaagga tatcaggtta taccgcttag aaaagatact ggaacgccaa tgattaagtt
      121 taaagatatc caaataacgg aagaagtgat caaaaataca aactggttta attgtgatta
      181 tgctttatta atgcgtggca tttggtgtat tgatattgat actcatgaca tggacgagaa
      241 gttagctaaa gagttataca taatgataaa aaagatgggg attgatttat tatctgtatt
      301 atcgactgat aagtatgaca atggactaga tggttattcg tcaattatta ggcatgagta
      361 taaaaacgaa ttaatcagta attttaaaaa tacattcgca gaattaactg caagtggtgg
      421 tatgcacata ctctttaaaa aacgcgatgg tattaattac acgcaaaaaa taggagtaat
      481 gcctggtgtt gacataaaag cgaatgataa taattttgtc aaaatattcc catctgacgg
      541 acgtgaagtt ttacaagcag ttaaaacatt accttattat gatggaaaat ttgaagagga
      601 agcatttaaa ccaaaacaag aaggtataac aacctatttt ggaggttctg tcacatatac
      661 atcaaacgga agtcacgaag gccgagaggc ttatgaacgt gtagcaactg gaacatcata
      721 taacaggaat aatgatttgt ttaaaggagc gtgttgggcg tttgaaaatg gtatcgatat
      781 tgatgattta acatcaatta ttgggacggt taaaggcaga gacgtattta cgagagagga
      841 gtttgaatta acgattgaat cagcgaaacg aaaagtcagc tacgtcacta tcagaacatg
      901 atatccaaaa ccttatccga atggaattat cacaagcggg gcatatggta tttcgagcaa
      961 atgtcggaaa ggtgaaaaca gcagatggta gattttttga tacaggttta ccaaaaggtt
     1021 tttgtgattt gtttggattt aaaccaaacg gacaaatatt ttttatcgaa gttaaaaatg
     1081 aaacaggtcg gataagacca gaacagaaaa aatttatgga ggtcatggca tctagaggag
     1141 cgcttgtagg agtggctcgg tcagtggaag atgccttaaa catagtcaat gacactagta
     1201 gatgattttt acaaacaaat ggagccgtca attaaagcgt ttttagacga taacattacc
     1261 atcgcagaca aagaagaagc tgacagagtc tatagatctg tcaaatacta taaaaaacta
     1321 aacagattgc cgccacctga tgtattggag tggttccaac gaatttacac gacaaaggaa
     1381 atgataatgt taatcaagca gtcttaccgc cttaaacaaa aaaagacaga tgaggatgac
     1441 aagatttacg aaaagtggat gtttaaaaac tacggtgacg ttaagctcgt taaaaaaatc
     1501 aaacgcacgg acgcactaga aagagctcgg agaatgggtc tatgaaaaga cacagacagt
     1561 ggcataacga tattaaatat acacctagat cttacgataa tctgttgcct tacgatatat
     1621 cagagctgtt aatagctcac agatgcaaaa taaagatgtc tgatgacgtt ttagcagata
     1681 agataggcat ttatacttgg caattaaaag cgctcttaga acgcagaata ttgccaaatg
     1741 agagcgagtg taaattgatt ataaattttt tgagagaggt ggagtgatga tagaagaatt
     1801 agtacataaa ccaaagcatt ataatcacgg agaaattgat ttgattgagt cgtggtataa
     1861 gacgtaccca tgggaccagt ttgtcgcaat catggaaagt cacattgata aatacattaa
     1921 acgacataga tacaaaggca atgcgactca ggatttagaa aaagcactcg aatatacaaa
     1981 gcgtctcatt gaatattggt taatcgaaag cgtggaagaa tgaagagata tattgaagtt
     2041 aaggacgaat ggaaaagtgc aacagaccac ctgaacgatt ttatcgacaa aaacaagtac
     2101 gcaaaagtga cagttgttgg ttatcaagtc gtacaacttt ctccttacgg aagagatttg
     2161 acttatattt tggcagaggt ggaagaatga caatagatga agcgttgcaa aatttacgtg
     2221 ataactttaa taaaataatg aatgtcctaa aaaacgattg gaaagcacta ttgtttcttg
     2281 caatcgcaat atttgggatg atggtaaccg tgtcgtattt tagctatcgc gacgcacgac
     2341 aatattacga gtcgcaaatc acaggactac gtacacagct aagcaggaca caaaagcagc
     2401 ttaaacgtgc tagcgaagat agagctagac agacaaagcg aattgcggaa tttacgcaca
     2461 acggagggta acatgattaa gatcgatgag atacatcgca tactaggcat cgacgaagtt
     2521 tataaagcac ccaaacgact tacggacata ctctttgata aagatagtcg tgaggatata
     2581 tttagacagt ttttgaaata tgaaacagat gtatcttacg actggtttat gcaatatttt
     2641 gaggaagaac aagctgaccg caaaaataaa aagcaagact ttacgcctaa atcggttagt
     2701 acactattat ctaaaataat aagtggtaat caatactacg aggtagcagt cgggacgggt
     2761 gggatactta tccaagcatg gcaagaacaa cgattaaatg acagtccatt tacttatcgt
     2821 ccgagtaaat actggtatca cgtagaagag ttatcggaca aagcagtacc gtttctactc
     2881 tttaatatgt ctataagagg gataaatggt gtggtggtgc atggtgactc tttaacaaga
     2941 caagttaaaa acatttattt tttgcaaaat acaaaagacg acatgctgag ttttagtgat
     3001 attaacgtta tgccaaggac tcaagatatt gagcaagaat ttaatgtcaa agagtggatt
     3061 ggtgatgcga tagtgcatgt agagagtaag ttaaatgcga ggtaacaaaa tgaacattga
     3121 agaagctatt aaaaagataa gagaactaga ggcgtttaat tatttcgact ttaaaggtaa
     3181 tttaattaga agaattgatg tattaggtat catcgaccaa ctaaaaatta tagtacaaca
     3241 atgtgttgca gattggtacg aaaagcataa ag
//
LOCUS       DATXIG010000097         3230 bp    DNA     linear   BCT 28-DEC-2023
DEFINITION  TPA_asm: Streptococcus pyogenes strain MGAS11113
            SAMN08555799-rid19682583.denovo.097, whole genome shotgun sequence.
ACCESSION   DATXIG010000097 DATXIG010000000
VERSION     DATXIG010000097.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN08555799
            Sequence Read Archive: SRR6740495
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pyogenes
  ORGANISM  Streptococcus pyogenes
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 3230)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3230)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-OCT-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-OCT-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001918058.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001918058.2
            Genome Coverage       :: 368x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/12/2023 11:23:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 1,806
            CDSs (total)                      :: 1,769
            Genes (coding)                    :: 1,722
            CDSs (with protein)               :: 1,722
            Genes (RNA)                       :: 37
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1, 1 (5S, 23S)
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 47
            CDSs (without protein)            :: 47
            Pseudo Genes (ambiguous residues) :: 0 of 47
            Pseudo Genes (frameshifted)       :: 30 of 47
            Pseudo Genes (incomplete)         :: 20 of 47
            Pseudo Genes (internal stop)      :: 9 of 47
            Pseudo Genes (multiple problems)  :: 11 of 47
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3230
                     /organism="Streptococcus pyogenes"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN08555799-rid19682583.denovo.097"
                     /strain="MGAS11113"
                     /serotype="Emm28"
                     /isolation_source="normally sterile site"
                     /host="Homo sapiens"
                     /db_xref="taxon:1314"
                     /country="Finland"
                     /collection_date="2002"
                     /collected_by="National Public Health Laboratory"
     gene            2..1972
                     /locus_tag="VC122_001661"
     CDS             2..1972
                     /locus_tag="VC122_001661"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003059944.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional metallophosphatase/5'-nucleotidase"
                     /protein_id="HEP5641564.1"
                     /translation="MTSFTLLVTDVQADQVDVQFLGVNDFHGALDNTGTAYTPSGKIP
                     NAGTAAQLGAYMDDAEIDFKQANQDGTSIRVQAGDMVGASPANSALLQDEPTVKVFNK
                     MKFEYGTLGNHEFDEGLDEFNRIMTGQAPDPESTINDITKQYEHEASHQTIVIANVID
                     KKTNDIPYGWKPYAIKDIAINDKIVKIGFIGVVTTEIPNLVLKQNYEHYQFLDAAETI
                     AKYAKELQEQHVHAIVVLAHIPATSKDGVVDHEMATVMEKVNQIYPEHSIDIIFAGHN
                     HQYTNGTIGKTRIVQALSQGKAYADVRGTLDTDTNDFMTTPSANVIAVAPGIKTENSD
                     IKAIINHANDIVKTVTERKIGTATNSSTISKTENIDKESPVGNLVTTAQLTIAKKTFP
                     TVDFAMTNNGGIRSDLVVKNDRTITWGAAQAVQPFGNILQVIQMTGQHIYDVLNQQYD
                     ENQTYFLQMSGLTYTYTDNDPKNSDTPFKIVKVYKDNGEEINLTTTYTVVVNDFLYGG
                     GDGFSAFKKAKLIGAINTDTEAFITYITNLEASGKTVNATIKGVKNYVTSNLESSTKV
                     NSAGKHSIISKVFRNRDGNIVSSEIISDLLTSTENTNNSLGKKETTTNKNTISSSTLP
                     ITGDNYKMSPIMTILALISLGGLNAFIKKRKS"
     gene            complement(2055..2465)
                     /locus_tag="VC122_001662"
     CDS             complement(2055..2465)
                     /locus_tag="VC122_001662"
                     /EC_number="3.5.1.88"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011017671.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide deformylase"
                     /protein_id="HEP5641565.1"
                     /translation="MIREIITDHFLLQQKAQVAKKEDLWIGQDLQDTLAFYRQECLGM
                     AANMIGEQKRIVIVSMGFIDLVMFNPVMVSKKGIYQTKESCLSLSGYRKTQRYDKITV
                     EYLDHNWRPKRLSLTGLTAQICQHELDHLEGILI"
BASE COUNT         1117 a          600 c          525 g          988 t
ORIGIN      
        1 cctgactagt tttactctat tagttacaga cgttcaagca gatcaagttg atgtgcaatt
       61 ccttggcgtc aatgattttc acggcgctct tgataatacc ggaacagctt acacaccaag
      121 tggtaaaata ccaaatgctg ggacggctgc tcaattaggt gcttatatgg atgacgctga
      181 gatagacttc aagcaagcaa atcaagacgg aacaagtata cgtgttcaag ctggagatat
      241 ggtcggagca agtcctgcta actctgcact tttacaagat gagcctactg taaaagtctt
      301 taacaaaatg aaatttgaat atggcactct tggtaatcat gaatttgacg aaggactaga
      361 tgaatttaac cgtatcatga caggtcaagc gcctgatcct gaatcaacaa ttaatgatat
      421 caccaaacaa tatgagcacg aagcttcgca tcaaaccatc gtcattgcta atgttattga
      481 taaaaaaacc aatgatatcc cctatggttg gaaaccttat gctataaaag acatagccat
      541 taatgacaaa atcgttaaga ttggcttcat tggtgttgtg actacagaga ttccaaatct
      601 cgttttaaag caaaactatg aacactatca atttttagat gcagctgaaa ccattgccaa
      661 atatgctaaa gaactacaag aacaacatgt tcatgctatt gtggttttag ctcatattcc
      721 tgcaacaagt aaagatggtg ttgttgatca tgaaatggct acggttatgg aaaaagtgaa
      781 ccaaatctat cccgaacata gcattgatat tatttttgca ggacataatc atcaatacac
      841 taatggaact atcggtaaaa cacgtatcgt tcaagctctc tctcaaggaa aagcttatgc
      901 agatgtccgt ggtacgctag atactgatac caatgatttt atgacaactc catcagcaaa
      961 tgttattgct gtagcaccag gtatcaaaac agaaaattca gatatcaaag ctataataaa
     1021 tcatgctaat gatattgtta aaacagttac tgaacgaaaa atcggaactg caactaattc
     1081 ttcaactatt tctaaaacag aaaatattga taaagaatct cctgtcggta acttagtaac
     1141 aacagctcag cttactattg ctaagaaaac ttttccaact gttgactttg ctatgaccaa
     1201 taatggtggt attcgaagtg acctagttgt caaaaatgat cggaccatca cctggggagc
     1261 tgcacaggct gtacaaccat ttggtaatat ccttcaagtc attcaaatga ctggtcaaca
     1321 catttacgat gtcctaaatc agcaatacga tgaaaaccag acctattttc ttcaaatgtc
     1381 aggtttaaca tacacttata cagataatga tcctaagaac tctgataccc ccttcaagat
     1441 agttaaggtt tataaagaca atggtgaaga aattaactta acaactactt acaccgttgt
     1501 tgtcaacgac tttctttatg gtggtggtga tggcttttca gcatttaaaa aagctaaatt
     1561 aattggagct attaacacag atactgaagc tttcatcaca tatatcacaa atttagaagc
     1621 atcaggtaaa actgttaatg ctactataaa aggggttaaa aattatgtaa cttcaaacct
     1681 tgaaagttcg acaaaagtta atagtgctgg taaacacagt atcattagta aggtttttag
     1741 aaatcgtgat ggcaatatag tgtctagtga gatcatttca gaccttttga cttctactga
     1801 aaacactaat aacagccttg gcaaaaaaga aacaacaaca aacaaaaata ctatctctag
     1861 ttccactctt ccaataacag gggacaatta taaaatgtct cctattatga caatccttgc
     1921 cttgataagc ttaggtggac taaacgcttt tattaaaaaa aggaaatcct agcgtttact
     1981 agtaatcgat cacccttata taatcaggca tctttgccaa aaaaaaccta aagcgtccta
     2041 ctctaggttt tttgttaaat taaaatacct tctagatgat ctaattcgtg ctggcatatt
     2101 tgagcagtta agcctgttaa gcttaatcgc tttggtcgcc aattatggtc caaatactca
     2161 acagtaatct tatcataccg ctgtgttttg cgatagccag ataacgacaa acaagattct
     2221 ttagtttggt aaattccctt tttagaaacc atcacaggat taaacataac caaatcaata
     2281 aatcccatac tcacaataac aatgcgcttt tgttcaccaa tcatattggc agccatccca
     2341 agacattcct gtctataaaa ggctaaggta tcttgtaaat cttggccaat ccatagatct
     2401 tctttcttgg cgacttgcgc tttttgctgt aataaaaaat gatctgttat gatttctcta
     2461 atcattctta tctccttaaa ccttcttttt aattcataaa ctacatgttc aatggactat
     2521 tatcaccata tgatagactt agtatcatct gtttaaaaca tcaggtgatt ccttatttca
     2581 agatatcttg ggcaacttta tgaaattctg caatatgagt accaaataaa aattgacctg
     2641 catcagcaga ttgttgctaa gttctcatca caatattatc gaatttactg tctgcttctt
     2701 caaatgtcta agagagatag ttattgcttt gtaagatttt caaaccagat actgctttct
     2761 cttgcatttg cagccttaac aggtccaaat aaaacacctg ctgtttgaaa tgctttaata
     2821 gctgatatgt tgttaatagt tagtattata aattttctga atatttctgt caagtttttc
     2881 agttgactta acttttttac ttgatttttc ctcacgtaat aatagaaaag aagcgccata
     2941 atgtaaacta caccccaaac gttagataaa aaaatctaac gtttggggtg tttttgtatg
     3001 acattaagct atgaagataa gctagatatt tagcgctaaa aaaaccgagg gaactccgtt
     3061 tgaaagaagc atctctgcaa agaagaagac aaaaaaccat tataaagaat ggacaaagac
     3121 gagttctgat tagattttct tttaagaacg gctaaattga ctcgttctac ctactattat
     3181 catcgattat tgcaacaata aacgtatcaa aataaaacga aaaggactta
//
LOCUS       DATXIG010000098         3219 bp    DNA     linear   BCT 28-DEC-2023
DEFINITION  TPA_asm: Streptococcus pyogenes strain MGAS11113
            SAMN08555799-rid19682583.denovo.098, whole genome shotgun sequence.
ACCESSION   DATXIG010000098 DATXIG010000000
VERSION     DATXIG010000098.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN08555799
            Sequence Read Archive: SRR6740495
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pyogenes
  ORGANISM  Streptococcus pyogenes
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 3219)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3219)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-OCT-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-OCT-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001918058.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001918058.2
            Genome Coverage       :: 368x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/12/2023 11:23:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 1,806
            CDSs (total)                      :: 1,769
            Genes (coding)                    :: 1,722
            CDSs (with protein)               :: 1,722
            Genes (RNA)                       :: 37
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1, 1 (5S, 23S)
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 47
            CDSs (without protein)            :: 47
            Pseudo Genes (ambiguous residues) :: 0 of 47
            Pseudo Genes (frameshifted)       :: 30 of 47
            Pseudo Genes (incomplete)         :: 20 of 47
            Pseudo Genes (internal stop)      :: 9 of 47
            Pseudo Genes (multiple problems)  :: 11 of 47
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3219
                     /organism="Streptococcus pyogenes"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN08555799-rid19682583.denovo.098"
                     /strain="MGAS11113"
                     /serotype="Emm28"
                     /isolation_source="normally sterile site"
                     /host="Homo sapiens"
                     /db_xref="taxon:1314"
                     /country="Finland"
                     /collection_date="2002"
                     /collected_by="National Public Health Laboratory"
     gene            19..762
                     /locus_tag="VC122_001663"
     CDS             19..762
                     /locus_tag="VC122_001663"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002984272.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HEP5641566.1"
                     /translation="MNILGNSIKEIRKSKKMTQKELAKLTGFKQNTISNHENGNRQLD
                     EVDIRKYAKALKIEPQQLFDYSSSPTNPQVELIPSTLQKINSTSSQLEHSRQIIVLDT
                     AETLLEQQKEIKNNEDTIAELFSYNYYDHAASAGTGQYLNDVQVEKIELPVDYDADFV
                     IPVYGDSMEPKYHSGDYVFVKLSVELTDGDIGVFEYYGDAYIKQLLINAEGAFLHSLN
                     QCGDYPDIPIDRDSDFRIIGEVMGSYRER"
     gene            775..1584
                     /locus_tag="VC122_001664"
     CDS             775..1584
                     /locus_tag="VC122_001664"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010922198.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system PemK/MazF family
                     toxin"
                     /protein_id="HEP5641567.1"
                     /translation="MDSKALNKQNLSRSKKILLNISDNPKNPKFYKLSRQELERAKIF
                     KTENLIKSQKYQRYKRGTIVFIHFGVNIGNEFSDSHFGIVLNKKDHPNNGKLTILPLT
                     SKNSKESLSINKEIFTSIMDDAEKTVQTIQNVLNLTTEVERIHHSLPVPPAFFQIKKE
                     NKYHDIWMKYYNRHDPKGIHVPVANITVRKWIQSDLDKINFLKKRYANYDKVSYAKLD
                     SITSVSKLKIAKPINDLDPVGKITLSKEIMDNIDKALARQLLSGQWGKLDN"
     gene            1834..2922
                     /locus_tag="VC122_001665"
     CDS             1834..2922
                     /locus_tag="VC122_001665"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012767061.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HEP5641568.1"
                     /translation="MKIKSYKKENGETAYKFLLYAGYVNGKRKYIRREGFKTKQAARE
                     TLISLQAELDKPKSSMTFGVLTKQWLNEYEKTVQGSTYLKTERNINKHILPKLDKVKI
                     GDINPLLIQRLTEEWCNDLKYGGKILGLVRNILNLAVRYGYINNNPALPITPPKIKRK
                     RKMNNNFYTLDQLKQFLELVEKTDNIEKIALFRLLAFTGIRKGELLALTWDDLNGNTL
                     SINKAVTRTQVGLEIDVTKTKSSDRLISLDDETLEILQELHETFPTSTLMFQSESGGI
                     MTPSLPRKWLLQIIKGTDLPQITIHGFRHTHASLLFESGLSLKQVQHRLGHGDLQTTM
                     NVYTHITQSAIDDIGTKFNQFVTNKQLD"
BASE COUNT         1203 a          580 c          542 g          894 t
ORIGIN      
        1 agagaaagga aaaatattat gaatattctc ggaaattcta tcaaagaaat tcgaaaatcc
       61 aaaaaaatga cccaaaaaga gttagcaaaa ctaactggat ttaaacaaaa tacaatttct
      121 aatcacgaaa atggtaatag acaacttgac gaagtagata ttcgaaaata tgctaaagca
      181 ctgaaaatag agccacaaca gctttttgac tattcctctt ctcccacaaa cccccaagtt
      241 gaactcatcc catctaccct acaaaaaata aactctactt cttctcaatt agaacacagt
      301 agacagataa ttgttttaga tacagctgag actttattgg aacaacagaa agaaattaaa
      361 aacaacgaag atactattgc cgaattattt tcttacaact actacgacca cgcagcttca
      421 gctggtacag gtcagtatct aaatgatgta caagtagaaa aaattgagtt accagtcgat
      481 tatgacgcag actttgttat ccctgtttat ggtgattcca tggaaccgaa gtatcactct
      541 ggggattatg tatttgttaa gctatccgta gagcttacag atggcgatat aggcgtcttt
      601 gaatactatg gtgacgctta tatcaaacag ttgcttataa acgctgaagg ggcgttttta
      661 cacagtttaa atcaatgcgg tgattatcca gatataccga tagatagaga tagcgacttt
      721 aggattattg gtgaagttat ggggagttat agggagagat aggcaggagc agaaatggat
      781 agtaaagcat tgaataaaca aaatttaagc cgtagcaaga aaattctgtt gaatatctct
      841 gataatccga aaaatcccaa attttacaaa ttatcaagac aagaactcga gcgcgctaaa
      901 atttttaaaa cagaaaactt aattaaatct caaaaatatc agagatacaa acgaggaact
      961 atcgtcttta ttcattttgg agtaaatatc ggcaatgaat tttccgattc gcactttggg
     1021 attgtgctga ataaaaaaga tcatcccaat aacggaaagc taactatctt accgctaaca
     1081 tcaaaaaact ctaaagagag tctgtcaatt aacaaagaga tttttacgag cataatggat
     1141 gatgccgaaa aaacagttca aacaattcaa aatgtgctaa acctaacaac tgaagtagaa
     1201 cggatacacc actcgttacc agttccccca gcgttttttc agattaaaaa agaaaataaa
     1261 tatcatgata tatggatgaa atattacaat aggcatgacc ctaaagggat tcatgtgccc
     1321 gtagcaaaca ttaccgtccg aaaatggata caatcagact tagacaaaat taacttcctc
     1381 aaaaaaagat atgcaaatta tgacaaggtg tcctacgcca agctggactc aatcacttct
     1441 gtcagtaaac taaaaatcgc taaaccaatt aacgatcttg accctgtcgg aaaaataaca
     1501 ttgtctaaag aaatcatgga caacattgat aaagctctcg caaggcagtt attatctggc
     1561 cagtggggga aacttgacaa ttaaatatca ctatgttaga attaaggtgt aatcttggta
     1621 gcctcgctac cactgaaaca ttattttgga gtgtccaact ccactgtgag cgcctgtttt
     1681 cgaataagcg ctcttttttg ttttaaaaaa ataaaaaagc cccacgctct caaagtttgg
     1741 cgactctgag cgtgaggcaa gacagtataa gaaacaacca ttaaaaaggt cattttcttg
     1801 tacctatttt atcaaattga aagatggtat gcaatgaaaa ttaaatcata taaaaaggaa
     1861 aatggtgaaa ctgcttataa atttcttttg tatgccggtt atgttaatgg aaagagaaaa
     1921 tatattaggc gagaaggttt caaaactaag caggctgcaa gggaaacctt aattagtttg
     1981 caagctgaac ttgataaacc taaatcaagt atgacgtttg gtgtgttaac taaacaatgg
     2041 ctaaacgaat atgaaaaaac tgtccagggc agtacctact taaaaacaga aagaaatatt
     2101 aataaacata ttttgccaaa acttgataaa gtgaagattg gagacatcaa cccactactt
     2161 atccagcggc ttactgaaga atggtgcaac gatttaaaat atggaggaaa aattcttggg
     2221 cttgttagga atatcttaaa tctagctgtt agatacggat atatcaataa caatccagct
     2281 ttgccaatta cacctccaaa aataaaaagg aaaagaaaaa tgaataataa tttttataca
     2341 cttgatcaac ttaaacaatt ccttgaacta gttgaaaaaa ctgacaacat tgaaaaaata
     2401 gccttgttta gattattagc atttactgga atacgaaaag gggagcttct ggcactaact
     2461 tgggatgatt tgaatggtaa tactctatca attaataaag ctgtcacacg tactcaagtt
     2521 ggactagaaa tagatgttac gaagacaaaa tcgagcgata gattaatcag cttagatgat
     2581 gaaactttgg aaattttaca agaacttcat gaaacttttc ctacttctac tcttatgttc
     2641 caatctgaat caggtggaat tatgacgcca agtttaccac gaaaatggct attgcaaatt
     2701 atcaaaggga cagacttacc acaaatcaca attcatggtt tcaggcacac tcatgcaagc
     2761 ttacttttcg aatcaggtct atccttgaaa caggtgcaac ataggttagg gcatggagat
     2821 ttacagacaa ctatgaacgt atatactcac atcacgcaat cggcaattga tgacattgga
     2881 actaaattca atcaatttgt tactaacaag caactagatt gacaactaat tctcaacaaa
     2941 cgttaattta acaacatcca agtaactccc accagctcca tcaatgctta ccgtaagtaa
     3001 tcataactta ctaaaacctt gttacatcaa ggttttttct ttttgtcttg ttcatgagtt
     3061 tccgttatcc ctatagccat accatcacgc ttaccgatac tccatcaata ccactcaaaa
     3121 ccttgtcata tcagatcttt ttgaccattt ttttcatgaa tatctaaaaa aaagaatcaa
     3181 aatagtacta aattccccat agcgcatgcg ctatgggga
//
LOCUS       DATXIG010000099         3179 bp    DNA     linear   BCT 28-DEC-2023
DEFINITION  TPA_asm: Streptococcus pyogenes strain MGAS11113
            SAMN08555799-rid19682583.denovo.099, whole genome shotgun sequence.
ACCESSION   DATXIG010000099 DATXIG010000000
VERSION     DATXIG010000099.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN08555799
            Sequence Read Archive: SRR6740495
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pyogenes
  ORGANISM  Streptococcus pyogenes
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 3179)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3179)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-OCT-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-OCT-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001918058.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001918058.2
            Genome Coverage       :: 368x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/12/2023 11:23:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 1,806
            CDSs (total)                      :: 1,769
            Genes (coding)                    :: 1,722
            CDSs (with protein)               :: 1,722
            Genes (RNA)                       :: 37
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1, 1 (5S, 23S)
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 47
            CDSs (without protein)            :: 47
            Pseudo Genes (ambiguous residues) :: 0 of 47
            Pseudo Genes (frameshifted)       :: 30 of 47
            Pseudo Genes (incomplete)         :: 20 of 47
            Pseudo Genes (internal stop)      :: 9 of 47
            Pseudo Genes (multiple problems)  :: 11 of 47
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3179
                     /organism="Streptococcus pyogenes"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN08555799-rid19682583.denovo.099"
                     /strain="MGAS11113"
                     /serotype="Emm28"
                     /isolation_source="normally sterile site"
                     /host="Homo sapiens"
                     /db_xref="taxon:1314"
                     /country="Finland"
                     /collection_date="2002"
                     /collected_by="National Public Health Laboratory"
     gene            671..1282
                     /gene="rpsD"
                     /locus_tag="VC122_001666"
     CDS             671..1282
                     /gene="rpsD"
                     /locus_tag="VC122_001666"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014407971.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S4"
                     /protein_id="HEP5641569.1"
                     /translation="MSRYTGPSWKQSRRLGLSLTGTGKELARRNYVPGQHGPNNRSKL
                     SEYGLQLAEKQKLRFSYGLGEKQFRNLFVQATKIKEGTLGFNFMVLLERRLDNVVYRL
                     GLATTRRQARQFVNHGHILVDGKRVDIPSYRVDPGQVISVREKSMKVPAILEAVEATL
                     GRPAFVSFDAEKLEGSLTRLPERDEINPEINEALVVEFYNKML"
     gene            complement(1439..2602)
                     /locus_tag="VC122_001667"
     CDS             complement(1439..2602)
                     /locus_tag="VC122_001667"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012767674.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific integrase"
                     /protein_id="HEP5641570.1"
                     /translation="MNIKEYKKKNGTIIYRTSVYLGVDQVTGKKARTTITASTKKGVK
                     IKARDALNNFAMNGYTVKEKPTVTTYKELTALWWESYKNTIKPNSRQSMEGIVRLHIL
                     PAFGDCKLSKLTTPVIQQQVNKWANNANKGIKGAYANYSFLNNINRRILQYGVTMQVI
                     EHNPARDVIIPRKQNNKEHKVKFFSNQELKQFLNYLDDLDLSSYENFFDYVLYKTLLA
                     TGCRIGEVLALEWSDIDLKKGTIKVSKTLNRYQETNTPKSKAGLRDIEIDRATVLLLK
                     QYKNRQQVLSWDLGRSETIVFTPFTTKYAYACLLRKRLQKHFKAAGVPDISFHGFRHT
                     HATIMLYAGIEAKDLQYRLGHSNISMTLNTYVHATKEGAKKAVSIFETAISNL"
     gene            complement(2782..>3179)
                     /locus_tag="VC122_001668"
     CDS             complement(2782..>3179)
                     /locus_tag="VC122_001668"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011285322.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HEP5641571.1"
                     /translation="QKDFFDQVVNKKLNLDISFRTYQNWENPKNTIKPDKAQILADYF
                     GVWLPYLLGNSNIRTEEQANFLAEMNKRINSEGDSFSFDMTATESDEHDLLYNYRQLE
                     TPYKNQVRKFTRDMWELQKESKNFSPKSP"
BASE COUNT          928 a          553 c          618 g         1080 t
ORIGIN      
        1 gattattctg cctgtattct ttttattttg aaaaaattcc tataaaagat aatattttta
       61 ggttagattg aaggaaaaaa gaggtcttca ccatggttaa gcaaatgatc ttagtactaa
      121 ggttaaaaga ttatcgtgaa ataaagtttt gacacaagtg taattaatag aggaacttga
      181 agtatccggt aaaactatct tgaagtgaaa aaacgagtag acctcccctg ataaatacaa
      241 tatactagcc atttcagagc aattaagagt ttccagtgaa gtgcttttga tagagagcta
      301 agaaaaaaac agtcaaggtt taagaactgc aaattagcat ttaaattatt ttttgttaat
      361 gacagttgtc tttattacca taactataac ttcttataat atagccttct taattcatca
      421 gtggtggttc gtcccatatc tgaccttaaa atattaaact gatcatcaga tgatagatag
      481 actgacttta caaaacaact ttcttatggt aaactgttct ttgtgcgaaa taacagcaga
      541 aaaatatgaa gctcgtcaac aggtggttat ctattcaagt aacctttgct gtttaggcga
      601 aaaccccctg cacgaatcag gattttttaa accgttattt cctcaaaaaa tttagaggag
      661 gacatgtaat atgtcacgtt atactggtcc atcatggaaa caatcacgtc gccttggttt
      721 atcacttaca ggcacaggta aagaattggc acgtcgtaac tacgttcctg gtcaacacgg
      781 tccaaacaac cgtagcaaac tctcagaata tggtttgcag ttagctgaga aacaaaaact
      841 tcgtttctca tacggtttag gtgaaaaaca attccgtaac ttgttcgttc aagctacaaa
      901 aatcaaagaa ggaactcttg gtttcaactt tatggttctt ttagaacgtc gtcttgacaa
      961 cgttgtttac cgtctaggtt tagcaactac tcgtcgtcaa gcacgtcaat tcgttaacca
     1021 cggtcacatc cttgttgacg gtaaacgcgt tgatatccct tcataccgtg ttgatccagg
     1081 tcaagtgatt tcagttcgcg aaaaatcaat gaaagtacct gcaatccttg aagctgttga
     1141 agctactctt ggacgtccag ctttcgtatc atttgatgct gaaaaacttg aaggttcatt
     1201 gactcgcctt ccagaacgcg atgaaattaa cccagaaatc aatgaagcac ttgtcgttga
     1261 attctataac aaaatgttgt aatattttat tgaataggat aggcttaaaa gccttgatac
     1321 taagcacttt ggggcattat tccaaggtgc tttttttgat tttactaccc ttttagttct
     1381 ctttagtcgg attttagtat agtaagaggg tagccccaaa gtggggcacc cttgttaatt
     1441 ataaattgct aatagctgtc tcaaaaatcg agacggcttt tttagcgcct tcttttgttg
     1501 catggacata agtattcaat gtcattgaga tattagagtg tcctagtcta tattgcaagt
     1561 ctttagcctc tatcccagcg taaagcataa ttgtagcgtg ggtgtggcgg aagccgtgga
     1621 aactaatatc aggcacgcca gcagctttaa aatgcttttg aagccttttt ctaagcaaac
     1681 aagcgtaggc gtattttgtg gtaaacggag taaaaacaat cgtctcagac cgtcctagat
     1741 cccaagataa aacttgctga cggtttttat attgcttaag aagtaagacg gtagctctat
     1801 caatctcaat gtctcttaat cctgcttttg atttaggagt gtttgtctcc tgatatctat
     1861 tcaaagtttt ggaaaccttg atagttcctt tttttagatc aatatcagac cattcaagag
     1921 ctagaacttc tccaatccga caaccagtag caagcaaggt cttataaaga acgtaatcaa
     1981 agaagttttc atagctagat aaatctaagt catctaaata gttcaagaat tgctttagtt
     2041 cttgattgct gaagaacttt accttatgtt ctttgttatt ttgttttcga ggaatgatga
     2101 cgtcacgagc tggattatgt tcaataactt gcatagtaac gccatattgt agaatacggc
     2161 gattgatatt atttagaaaa ctgtagttag catatgcgcc tttaatgccc ttattagcgt
     2221 tgttagccca cttgttaact tgttgctgaa taacaggggt agttagctta gatagcttgc
     2281 aatccccaaa tgcgggcaaa atatgaagtc ttacaatccc ttccatagat tgccgagagt
     2341 ttggtttgat cgtattcttg taactctccc accaaagggc tgttagctct ttataagtgg
     2401 ttactgtcgg cttctctttg acggtatagc cattcatagc aaagttattt agagcatcac
     2461 gagcttttat tttaacccct tttttagttg aagctgtaat agttgtgcga gccttcttac
     2521 ctgttacctg gtcgacacca agatagacac tagtgcggta aatgattgta ccgttcttct
     2581 ttttgtattc ttttatgttc atgataatac ctttctaaca tcagtaagca agtatgggat
     2641 ttagttaggt atttaggttt tatcatgggt gatagttggt tatctggtag ggtggcgata
     2701 aggtagaggg tgtgttaatg ctatggttgt caatataagt gttgattatt gtttggtggg
     2761 atagttgcga gctggtcggt atcagggaga ttttggagag aagtttttgc tttctttttg
     2821 caactcccac atgtctctag taaattttct aacttgattt ttatagggag tttcaagctg
     2881 tctgtaatta tataataggt cgtgttcatc agattcggtt gcggtcatgt cgaaggaaaa
     2941 tgaatcacct tcagagttaa ttcgcttgtt catttctgct aaaaaattcg cttgctcttc
     3001 tgttcggata ttgctatttc ctagaagata agggagccat actccaaaat agtccgccag
     3061 tatttgagct ttatcaggct tgattgtatt ctttgggttt tcccaatttt gatatgttct
     3121 aaatgaaatg tctagattta atttcttatt tacgacttga tcaaaaaaat ctttttgat
//
LOCUS       DATXIG010000100         2896 bp    DNA     linear   BCT 28-DEC-2023
DEFINITION  TPA_asm: Streptococcus pyogenes strain MGAS11113
            SAMN08555799-rid19682583.denovo.100, whole genome shotgun sequence.
ACCESSION   DATXIG010000100 DATXIG010000000
VERSION     DATXIG010000100.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN08555799
            Sequence Read Archive: SRR6740495
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pyogenes
  ORGANISM  Streptococcus pyogenes
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 2896)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2896)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-OCT-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-OCT-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001918058.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001918058.2
            Genome Coverage       :: 368x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/12/2023 11:23:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 1,806
            CDSs (total)                      :: 1,769
            Genes (coding)                    :: 1,722
            CDSs (with protein)               :: 1,722
            Genes (RNA)                       :: 37
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1, 1 (5S, 23S)
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 47
            CDSs (without protein)            :: 47
            Pseudo Genes (ambiguous residues) :: 0 of 47
            Pseudo Genes (frameshifted)       :: 30 of 47
            Pseudo Genes (incomplete)         :: 20 of 47
            Pseudo Genes (internal stop)      :: 9 of 47
            Pseudo Genes (multiple problems)  :: 11 of 47
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2896
                     /organism="Streptococcus pyogenes"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN08555799-rid19682583.denovo.100"
                     /strain="MGAS11113"
                     /serotype="Emm28"
                     /isolation_source="normally sterile site"
                     /host="Homo sapiens"
                     /db_xref="taxon:1314"
                     /country="Finland"
                     /collection_date="2002"
                     /collected_by="National Public Health Laboratory"
     gene            178..2010
                     /gene="lepA"
                     /locus_tag="VC122_001669"
     CDS             178..2010
                     /gene="lepA"
                     /locus_tag="VC122_001669"
                     /EC_number="3.6.5.n1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017645216.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor 4"
                     /protein_id="HEP5641572.1"
                     /translation="MNSQDLKKRQEKIRNFSIIAHIDHGKSTLADRILEKTETVSSRE
                     MQAQLLDSMDLERERGITIKLNAIELNYTAKDGETYIFHLIDTPGHVDFTYEVSRSLA
                     ACEGAILVVDAAQGIEAQTLANVYLALDNDLEILPVINKIDLPAADPERVRHEVEDVI
                     GLDASEAVLASAKAGIGIEEILEQIVEKVPAPTGDVDAPLQALIFDSVYDAYRGVILQ
                     VRIVNGIVKPGDKIQMMSNGKTFDVTEVGIFTPKAVGRDFLATGDVGYVAASIKTVAD
                     TRVGDTVTLANNPAKEALHGYKQMNPMVFAGIYPIESNKYNDLREALEKLQLNDASLQ
                     FEPETSQALGFGFRCGFLGLLHMDVIQERLEREFNIDLIMTAPSVVYHVHTTDEDMIE
                     VSNPSEFPDPTRVAFIEEPYVKAQIMVPQEFVGAVMELSQRKRGDFVTMDYIDDNRVN
                     VIYQIPLAEIVFDFFDKLKSSTRGYASFDYDMSEYRRSQLVKMDILLNGDKVDALSFI
                     VHKEFAYERGKIIVEKLKKIIPRQQFEVPIQAAIGQKIVARSDIKALRKNVLAKCYGG
                     DVSRKRKLLEKQKAGKKRMKAIGSVEVPQEAFLSVLSMDDDAKK"
     gene            2268..>2896
                     /locus_tag="VC122_001670"
     CDS             2268..>2896
                     /locus_tag="VC122_001670"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011054452.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="collagen-like protein"
                     /protein_id="HEP5641573.1"
                     /translation="MLTFGGASAVKADEQEEKAKVRTELIQELAQGLGGIEKKNFPTL
                     GDEDLDHTYMTKLLTYLQEREQAENSWRKRLLKGIQDHALDGQDGRNGERGEQGPTGL
                     AGKAGEAGAKGETGPAGPQGPRGEQGPQGLPGKDGEAGAQGPAGPMGPAGERGEKGEP
                     GTQGAKGDRGETGPVGPRGERGEAGPAGKDGERGPVGPAGKDGQNGQDGL"
BASE COUNT          867 a          535 c          729 g          765 t
ORIGIN      
        1 agaggctaag cagatgattg atttaaaaag gaatcttttc aaaattgatg tccgggcaca
       61 aaaggatgaa gagaaagttt tcatgcgtac ggcgtggata atccgtaaga aaagataggc
      121 aaaggcctac cttttttgtt ataatagaag gaaagactaa atgtatagga aaatagaatg
      181 aacagtcaag atttaaaaaa acgtcaggag aagattcgta atttctccat tattgcacat
      241 attgaccatg gcaaatctac tctcgctgac cgtattttgg aaaagacaga gacggtttcg
      301 tctcgtgaaa tgcaggctca gttattggac tccatggatt tggagcgtga gcgtgggatt
      361 accatcaagt taaacgccat tgagcttaat tacacggcta aagatgggga aacctacatt
      421 ttccacctta ttgatacccc agggcatgtg gactttactt atgaagtatc gcgttcgttg
      481 gcagcctgtg agggagcgat tctagttgtg gatgcagcgc agggcattga agcacagacc
      541 cttgccaatg tttacctagc ccttgataat gatttggaaa ttttaccggt tatcaataaa
      601 attgacttgc ctgctgctga ccctgagaga gtccgccatg aagtagaaga tgtcattgga
      661 cttgatgctt ctgaggctgt tctggcgtca gccaaggctg gtattgggat cgaagagatt
      721 cttgagcaaa ttgttgagaa ggttcctgct cctacgggtg atgtggatgc gcctttacag
      781 gccttgattt ttgactctgt gtatgatgct taccgagggg taattttgca ggttcggatt
      841 gtgaacggta ttgtcaagcc tggcgataaa atccagatga tgtcgaatgg aaaaaccttt
      901 gatgttacag aagttgggat tttcactcct aaggcagttg gacgagattt ccttgcgacg
      961 ggagatgttg gttatgttgc ggcctctatc aaaacggtag cagatacccg tgtgggcgat
     1021 acggtgactt tagctaataa ccctgctaag gaagccttgc atggctacaa gcagatgaac
     1081 ccgatggtct ttgcagggat ttatccgatt gaatcaaata aatacaatga cttgcgtgaa
     1141 gcgcttgaaa aattacaact gaacgatgcc agtttacagt ttgaacccga aacgtcacaa
     1201 gcgcttggct ttggttttcg atgcggtttt ttaggcttgc ttcacatgga tgtgattcaa
     1261 gaacgtttgg aacgtgaatt taacattgat ttgatcatga cagcaccgtc cgtggtgtat
     1321 cacgttcaca caacagatga ggacatgatt gaagtgtcta acccttcaga atttccagat
     1381 ccaacacggg tggcttttat tgaagagcct tatgttaaag cgcaaatcat ggtgccacaa
     1441 gagttcgtag gggctgttat ggaattatcg cagcgcaagc gtggtgattt tgtgaccatg
     1501 gattacattg atgataaccg tgtcaatgtg atctaccaaa tcccgctggc tgaaattgtc
     1561 tttgatttct ttgataaatt gaaatcctca acgcgaggct atgcgagttt tgattatgac
     1621 atgtcagaat accgcaggtc acaattggtg aaaatggaca ttcttttgaa cggtgataag
     1681 gtcgatgcgc tcagctttat tgttcataaa gaatttgctt atgaacgcgg gaaaattatt
     1741 gttgagaaat tgaaaaaaat cattccacgt caacagtttg aagtgcctat tcaagccgct
     1801 attggtcaaa aaattgttgc gcgttcagac atcaaggccc ttcgtaaaaa cgttttagcg
     1861 aagtgttatg gtggtgacgt gtctcgtaaa cgtaaattgc tcgaaaaaca aaaggctggt
     1921 aaaaaacgga tgaaggctat tggttctgtt gaagtccctc aagaagcctt cttgagtgtt
     1981 ctttcaatgg atgacgatgc caagaaataa ccaagcctaa tcgcttagtc ttcagaggag
     2041 aactcttgtg ggtctacatg accaaaaaat ggttatctta tacaaaaaga actttacaat
     2101 catttgcaca taatgttata ataatattat agtgttatct cattattagg tgcgcctgca
     2161 tttaatctta ttaaacagaa agaggaaaca aaaactgtga acaaaacaaa acaaaaacaa
     2221 aacatagcct tctttgtcgc tatggcttaa cctcagcggc tgctcttttg ctgacctttg
     2281 gaggtgcaag tgcagttaag gcggatgaac aagaagagaa agctaaagtt agaactgaat
     2341 taattcaaga gttagctcag ggactagggg gtattgagaa aaaaaatttt ccaactctag
     2401 gtgatgaaga tttagatcat acttatatga caaagctatt aacataccta caggaacgag
     2461 aacaagctga gaatagttgg cgaaaaagac tactaaaggg tatacaagat catgcccttg
     2521 atggacaaga cggtcgaaac ggtgaaagag gggaacaagg accaacaggt ctcgctggta
     2581 aagccggtga agctggagcc aaaggcgaaa ccggccccgc tggtccacag ggtccacgtg
     2641 gtgaacaagg cccgcaaggt cttccaggta aagatggtga agctggtgct caaggcccag
     2701 caggtccaat gggtcctgct ggtgagcgag gtgaaaaagg agaacctggt acccaaggcg
     2761 ctaaaggtga tcgcggtgaa accggtccag taggtccacg tggtgagcga ggcgaagccg
     2821 gtcccgctgg aaaagatggt gaacgtggtc cagtaggtcc agctggtaag gacggccaaa
     2881 acggccaaga tggtct
//
LOCUS       DATXIG010000101         2650 bp    DNA     linear   BCT 28-DEC-2023
DEFINITION  TPA_asm: Streptococcus pyogenes strain MGAS11113
            SAMN08555799-rid19682583.denovo.101, whole genome shotgun sequence.
ACCESSION   DATXIG010000101 DATXIG010000000
VERSION     DATXIG010000101.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN08555799
            Sequence Read Archive: SRR6740495
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pyogenes
  ORGANISM  Streptococcus pyogenes
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 2650)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2650)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-OCT-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-OCT-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001918058.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001918058.2
            Genome Coverage       :: 368x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/12/2023 11:23:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 1,806
            CDSs (total)                      :: 1,769
            Genes (coding)                    :: 1,722
            CDSs (with protein)               :: 1,722
            Genes (RNA)                       :: 37
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1, 1 (5S, 23S)
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 47
            CDSs (without protein)            :: 47
            Pseudo Genes (ambiguous residues) :: 0 of 47
            Pseudo Genes (frameshifted)       :: 30 of 47
            Pseudo Genes (incomplete)         :: 20 of 47
            Pseudo Genes (internal stop)      :: 9 of 47
            Pseudo Genes (multiple problems)  :: 11 of 47
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2650
                     /organism="Streptococcus pyogenes"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN08555799-rid19682583.denovo.101"
                     /strain="MGAS11113"
                     /serotype="Emm28"
                     /isolation_source="normally sterile site"
                     /host="Homo sapiens"
                     /db_xref="taxon:1314"
                     /country="Finland"
                     /collection_date="2002"
                     /collected_by="National Public Health Laboratory"
     gene            complement(1..641)
                     /locus_tag="VC122_001671"
                     /pseudo
     CDS             complement(1..641)
                     /locus_tag="VC122_001671"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000191693.1"
                     /note="frameshifted; incomplete; missing C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
     gene            960..1187
                     /locus_tag="VC122_001672"
     CDS             960..1187
                     /locus_tag="VC122_001672"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000379879.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Blp family class II bacteriocin"
                     /protein_id="HEP5641574.1"
                     /translation="MEIKKLETFHQMTIEKLAKVEGGKNNWQANVSGVIAAGSAGAAI
                     GFPVCGVACGYIGAKTAITLWAGVTGATGGF"
     gene            1200..1400
                     /locus_tag="VC122_001673"
     CDS             1200..1400
                     /locus_tag="VC122_001673"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010921972.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class IIb bacteriocin, lactobin A/cerein 7B
                     family"
                     /protein_id="HEP5641575.1"
                     /translation="MTTMKELTINDMASISGGNAPGDAVIGGLGGLASGLKFCKLPHP
                     VLAGGCVVGFTVGGAYLGYTAN"
     gene            2414..>2650
                     /locus_tag="VC122_001674"
     CDS             2414..>2650
                     /locus_tag="VC122_001674"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017771275.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEP5641576.1"
                     /translation="MRNILRFLGIVIISAVILFSIGSFYDLPLMKNIQLICWSFILDL
                     FVFVYKQRQTTEVLTWYQVVKQFWLFIKCTILIPT"
BASE COUNT          849 a          378 c          526 g          897 t
ORIGIN      
        1 tttatgaata cgagctccga acttaatatt atagtcctcg aaaatactta caagacatcg
       61 ttctaaggct tcttcatttg ggaaaaccac tctcttcttg ttttgtcgct taatttcttt
      121 attgagtgat tcaatcaagt ttgtagagta aatgctagac caaatgtgat gtgggaattg
      181 atagaaagta aggagatttt ccgtcgtttc aagtgatgcc atgacctttt tataacgagg
      241 cttccattca gaaacaagtg gtgaccatac atgcgctcaa taatgtcact gatttcgcgt
      301 gtcgtaacgc cagtctgata gagtttgatc accatctctt ccaagtggtc atctcgtcta
      361 gcataagatg gtaataaaac tggtgaaaac tcgccgtttc gatctcttgg gatgattaaa
      421 ttgactaagc catactttgt ttcaaactga cgagaataag tcccattacg gctattacct
      481 gtgttgtaac cttctttttc ataaggttca taaccaagaa aagctgatag ttcaacttgc
      541 aagaggtcat tcatagctat ttctaaagag gagcgaaaga attcatcaat atcttgtttt
      601 tgtgctagga agttaagtaa ttctgtggta aactgagtca tgggaataaa tctctttcta
      661 gtgaagtgtc gtaactctac tatacaagat ttattccttt ttgtgtgttt acacaagata
      721 ttttacacta ccatcaaaaa tcttaaaagg tatttataaa agcatgaatg gtatcattta
      781 aacttcttaa ttaattaact attgtcacag cgtaagtgta gagaggataa aaagacattt
      841 catgatgaaa aatagccttt taagattttg aaaccatgat actgttcttt atgatattct
      901 gtactagctc gaggttgcaa cgagacaaat ttaagaatta tgaaaagaag ttattcgata
      961 tggagataaa gaaactggaa acatttcacc aaatgactat agaaaagctt gccaaggttg
     1021 agggtggtaa gaataattgg caggcaaatg tcagtggggt tatcgctgca ggtagtgctg
     1081 gagctgctat aggttttcca gtttgcggtg tagcctgtgg ttatattgga gctaaaacag
     1141 ctattactct ttgggctggt gtaacaggag ctacaggcgg cttctagagg aaagaaagga
     1201 tgacaacaat gaaggaatta acaattaatg acatggctag tataagtgga ggaaacgctc
     1261 caggagatgc tgtaattggt ggtcttggag gcttagcttc tggattgaaa ttttgtaaac
     1321 tgccacatcc agtattggca ggtggatgtg ttgtaggatt tacagttggt ggagcatatt
     1381 tgggatatac agcaaattaa atgatctata cgatttttat tattatgaat gcgtctataa
     1441 tgtttttaat cattgttgat gccattcata aaaaaatgtt gataaatcta taccatattt
     1501 agtgttttta attttcaatc aattatccct ttcggaaaaa tcggtaaaca tgttatctat
     1561 cgtagctttg gtagcgtcat tatttgtttt tatcgataaa agaaaatagt gattatgcca
     1621 atgaatttag atatcactct tagcttttta aaatacaaaa gagtgaaatt agaatgaaaa
     1681 agacttcaaa aatatggaag tgctaaaaaa atggtctgac tatcataaat ggaggcggaa
     1741 aagacggagc aaatatcttt ttgacaggga tggcaggtgc tgcgcaggga gtgacagttt
     1801 gtgctcagac aggcgtattt atcccttggc aaggttatat tttgtgtgga gcagctggtg
     1861 ctgctacaaa tattatctgg cctcactaaa tatataaatg actatctatc actgatatat
     1921 aagtagtaga ggagtggtat gaaaatttta gatattattt ttaacctata tgatagaaag
     1981 atgatatttc tagaaaatgt tgaaaagctg ggaggttaca ttgtcccttt tacccttatc
     2041 atcttgagtg tagcctacaa ctttaggcac ccttatccta tttgggctgc tgtactagga
     2101 ggtatatccc ttattgtgat ctttatttat tttatactta ttgtttatct atatttgcgt
     2161 tatagatact tggtgtggaa aaaaatacca gaaattaaga ttaattctac tgagctatca
     2221 tgcaatcaca tttaagggga aaatggatta cttatacaat atcagtactt atgatattta
     2281 actttagaga ctaaagagtt aaaagcaaaa tacgtattga aaattggtta aaaaagttat
     2341 aactgtctct gataacagga ataataattt gttcggttat atttgctacc gatagagtag
     2401 aggaggtgaa gatatgcgta atatattaag atttttaggt attgttatta tttctgcagt
     2461 gattctattt agtataggta gtttctacga tttaccatta atgaaaaata tacaacttat
     2521 ctgttggagt tttatccttg atttattcgt ttttgtctac aaacaaagac aaacgacaga
     2581 agtgctcaca tggtatcaag ttgttaagca attttggtta tttattaagt gtacaattct
     2641 tattcctacc
//
LOCUS       DATXIG010000102         2644 bp    DNA     linear   BCT 28-DEC-2023
DEFINITION  TPA_asm: Streptococcus pyogenes strain MGAS11113
            SAMN08555799-rid19682583.denovo.102, whole genome shotgun sequence.
ACCESSION   DATXIG010000102 DATXIG010000000
VERSION     DATXIG010000102.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN08555799
            Sequence Read Archive: SRR6740495
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pyogenes
  ORGANISM  Streptococcus pyogenes
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 2644)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2644)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-OCT-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-OCT-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001918058.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001918058.2
            Genome Coverage       :: 368x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/12/2023 11:23:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 1,806
            CDSs (total)                      :: 1,769
            Genes (coding)                    :: 1,722
            CDSs (with protein)               :: 1,722
            Genes (RNA)                       :: 37
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1, 1 (5S, 23S)
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 47
            CDSs (without protein)            :: 47
            Pseudo Genes (ambiguous residues) :: 0 of 47
            Pseudo Genes (frameshifted)       :: 30 of 47
            Pseudo Genes (incomplete)         :: 20 of 47
            Pseudo Genes (internal stop)      :: 9 of 47
            Pseudo Genes (multiple problems)  :: 11 of 47
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2644
                     /organism="Streptococcus pyogenes"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN08555799-rid19682583.denovo.102"
                     /strain="MGAS11113"
                     /serotype="Emm28"
                     /isolation_source="normally sterile site"
                     /host="Homo sapiens"
                     /db_xref="taxon:1314"
                     /country="Finland"
                     /collection_date="2002"
                     /collected_by="National Public Health Laboratory"
     gene            17..631
                     /locus_tag="VC122_001675"
     CDS             17..631
                     /locus_tag="VC122_001675"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011185103.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transglycosylase SLT domain-containing protein"
                     /protein_id="HEP5641577.1"
                     /translation="MFKKENLKQRYFNFGLVALALTILAIIFAFSSKNADTKSYAKKS
                     ESKMVTIDKAPKNNHAITKEESKDKSKSIASEPIPTVENSVAPTVTEEAPVVQQEVTQ
                     TVQQVSSVAYNPNNVVLSNGNTAGIVGSQAAAQMAAATGVPQSTWEHIIARESNGNPN
                     AANASGASGLFQTMPGWGSTATVEDQVNAALKAYSAQGLSAWGY"
     gene            complement(1045..1917)
                     /gene="sdaAA"
                     /locus_tag="VC122_001676"
     CDS             complement(1045..1917)
                     /gene="sdaAA"
                     /locus_tag="VC122_001676"
                     /EC_number="4.3.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000500148.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-serine ammonia-lyase, iron-sulfur-dependent,
                     subunit alpha"
                     /protein_id="HEP5641578.1"
                     /translation="MFYTIEELVKQADQQFNGNIAELMIATEVEMSGRNREDIIKIMS
                     RNLQVMKAAVTEGLTSTKSISGLTGGDAVKMDNYIKKGNSLSDTTILNAVRNAIAVNE
                     LNAKMGLVCATPTAGSAGCLPAVLATAIEKLDLSEKEQLEFLFTAGAFGLVIGNNASI
                     SGAEGGCQAEVGSAAAMSAAALVKAAGGTSHQASQAIAFVIKNLLGLVCDPVAGLVEV
                     PCVKRNALGASFALVAADMALADIDSQIPVDEVIDAMYQVGSAMPTAFRETAEGGLAA
                     TPTGRRYSVEIFGE"
     gene            complement(1929..2600)
                     /gene="sdaAB"
                     /locus_tag="VC122_001677"
     CDS             complement(1929..2600)
                     /gene="sdaAB"
                     /locus_tag="VC122_001677"
                     /EC_number="4.3.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000681580.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-serine ammonia-lyase, iron-sulfur-dependent
                     subunit beta"
                     /protein_id="HEP5641579.1"
                     /translation="MNTQKFQSVFDIIGPVMIGPSSSHTAGAVRIGKVVHSIFGDIPD
                     EVTFHLYNSFAKTYRGHGTDKALVAGIMGMDTDNPDIKNSLEIAHQKGIKIYWDILKD
                     SNAPHPNTVKISVKKADKTLSVTGISIGGGNIQVTELNGFSVSLSMNTPTIVTVHKDI
                     PGMIAKVTDILSSNNINIATMNVTRESAGEKATMIIEVDSRECQEAANQIAKIPHIYN
                     VNFFD"
BASE COUNT          834 a          495 c          487 g          828 t
ORIGIN      
        1 agtgaggtat tattgaatgt ttaagaaaga aaatttaaaa caacgttatt ttaattttgg
       61 attagtagcg ttagctctaa caatattagc catcattttt gccttctcaa gtaaaaatgc
      121 tgatactaag tcttatgcta agaagtcaga aagtaaaatg gtaacaatcg acaaggctcc
      181 aaaaaataat catgctatta ctaaagaaga aagcaaagat aaatcaaaga gcattgcttc
      241 ggagcctatt cccacagtag aaaactctgt agctccgaca gtaacagagg aagcaccggt
      301 tgttcagcaa gaagtgactc aaactgttca gcaggtatct tcagtagcct ataatccaaa
      361 caatgtggta ctttccaatg gaaatactgc tggtattgta ggaagtcaag cggcggcaca
      421 gatggcagca gcaacaggtg ttccacaatc aacttgggaa catataattg cgcgtgaatc
      481 taatggaaat cctaacgcag ctaatgcttc tggggcatca gggttgttcc agacaatgcc
      541 aggttggggt tctacagcaa cggttgaaga tcaagtcaat gcagccttga aagcctatag
      601 tgcacaaggt ttatcagctt ggggttacta aataaacata gctggaagaa ataaaaccta
      661 ttgttagtat ttactaacaa taggtttttg gtatttaatt gtctttatga tttgtagtga
      721 tgaattagtc tcaaaacaaa acattgcctt atcagggaat atagctcgta ttatttagga
      781 aaactgacca gcacttaagt gcctctgata tttaaggcag ttatactgat aacaagcagg
      841 aaaaaaactc ttaaatgttt ataagatcat tagcaatttt cagtatttta cactactaca
      901 cttttgagtt ttaacaattt actagaagtt gtttctaagg ggaaacaaca tagcttgata
      961 gctatctatt agtcatttta gtgaatatat agcagaaaaa taaaacgctt tcaaatattt
     1021 ctaagaattt ttctaactat ctatttattc gccaaatatt tctacgctgt aacgtcgtcc
     1081 agttggagta gcggctaaac caccttctgc tgtttcacgg aaagcagttg gcatggcaga
     1141 gccgacttga tacatagcat caataacttc gtcaacagga atttgagaat caatatcagc
     1201 taaagccata tcagctgcga caagggcgaa acttgctcct agagcattgc gtttgacaca
     1261 aggcacttct actaggccgg caacaggatc acagacaagt cctaataaat ttttgataac
     1321 aaacgcaata gcttgactag cttgatgaga tgttccacca gctgctttaa caagagcagc
     1381 tgcactcata gcagctgcag aaccaacttc tgcttggcat ccaccttctg caccagaaat
     1441 agaggcgtta tttccaataa caagaccaaa agctcctgca gtaaataaaa actctaattg
     1501 ttctttttct gataagtcaa gtttttcaat agctgttgcg agcactgctg gtaagcatcc
     1561 tgcacttcct gcagtgggtg ttgcacagac aagtcccatt ttagcattta attcgtttac
     1621 agctatagca tttctaacag cattaagaat agtagtatca gaaaggctgt tccctttttt
     1681 aatgtagtta tccattttga ccgcatcgcc ccctgtcaaa ccactgatag atttggttga
     1741 ggttagtcct tctgttacag ctgctttcat aacttgtaaa ttgcgtgaca tgattttgat
     1801 aatatcttct cgatttcttc cagacatctc cacttccgtg gcaatcatga gttcagcaat
     1861 atttccatta aattgttggt cagcttgttt gacaagttct tcaatagtat aaaacatagc
     1921 tttcctttct agtcaaagaa gttgacatta taaatatgag gaatttttgc aatttggttg
     1981 gctgcttctt ggcactctct agaatcgact tctataatca tagtagcttt ttcaccagcg
     2041 gattcacgtg taacattcat tgtggcgata ttaatattat tggaagatag gatatcagtt
     2101 acctttgcga tcattccggg aatatctttg tgtacagtaa ctatagtagg ggtattcatg
     2161 gataacgaga cagaaaaacc atttagttct gttacttgaa tgtttcctcc tccaattgaa
     2221 ataccagtaa cactaagtgt tttgtcagct tttttgacac tgattttaac agtattggga
     2281 tggggagcat tactatcttt taagatatcc caatagattt ttatcccttt ttgatgagca
     2341 atttctagag aatttttaat atcaggatta tcagtatcca tacccataat tcctgcaaca
     2401 agagccttat cagtaccatg tcctctgtag gttttagcaa atgaattata aaggtgaaac
     2461 gtcacttcat caggaatatc tccaaagatg gaatggacta ccttaccaat tctgacggca
     2521 ccagctgtgt gactacttga tggaccaatc attactggcc caatgatgtc aaaaactgat
     2581 tgaaattttt gtgtattcat aggcaactcc gttttttatt tctatgttta ttataacaaa
     2641 aaaa
//
LOCUS       DATXIG010000103         2523 bp    DNA     linear   BCT 28-DEC-2023
DEFINITION  TPA_asm: Streptococcus pyogenes strain MGAS11113
            SAMN08555799-rid19682583.denovo.103, whole genome shotgun sequence.
ACCESSION   DATXIG010000103 DATXIG010000000
VERSION     DATXIG010000103.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN08555799
            Sequence Read Archive: SRR6740495
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pyogenes
  ORGANISM  Streptococcus pyogenes
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 2523)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2523)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-OCT-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-OCT-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001918058.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001918058.2
            Genome Coverage       :: 368x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/12/2023 11:23:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 1,806
            CDSs (total)                      :: 1,769
            Genes (coding)                    :: 1,722
            CDSs (with protein)               :: 1,722
            Genes (RNA)                       :: 37
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1, 1 (5S, 23S)
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 47
            CDSs (without protein)            :: 47
            Pseudo Genes (ambiguous residues) :: 0 of 47
            Pseudo Genes (frameshifted)       :: 30 of 47
            Pseudo Genes (incomplete)         :: 20 of 47
            Pseudo Genes (internal stop)      :: 9 of 47
            Pseudo Genes (multiple problems)  :: 11 of 47
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2523
                     /organism="Streptococcus pyogenes"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN08555799-rid19682583.denovo.103"
                     /strain="MGAS11113"
                     /serotype="Emm28"
                     /isolation_source="normally sterile site"
                     /host="Homo sapiens"
                     /db_xref="taxon:1314"
                     /country="Finland"
                     /collection_date="2002"
                     /collected_by="National Public Health Laboratory"
     gene            71..484
                     /gene="yaaA"
                     /locus_tag="VC122_001678"
     CDS             71..484
                     /gene="yaaA"
                     /locus_tag="VC122_001678"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002992306.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S4 domain-containing protein YaaA"
                     /protein_id="HEP5641580.1"
                     /translation="MIYKLFTEFITLQALLKELGIIQSGGAIKGFLAETTVLFNGEDE
                     KRRGKKIRIGDKISLPDQDLIITIVEPSQEEKEQFAEEIAEKTRVAALVKQMNQANKK
                     TSSKHKNRQSTTKKSLRATKKTKGKPTAPVRFPGI"
     gene            486..1592
                     /gene="recF"
                     /locus_tag="VC122_001679"
     CDS             486..1592
                     /gene="recF"
                     /locus_tag="VC122_001679"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014407981.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA replication/repair protein RecF"
                     /protein_id="HEP5641581.1"
                     /translation="MWIKELELKHYRNYDHLLASFSSGLNVFIGNNAQGKTNFLEAIY
                     FLSLTRSHRTRADKELIHFDHSTVSLTGKIQRISGTVDLEINLSDKGRVTKINALKQA
                     KLSDYIGTMMVVLFAPEDLQLVKGAPSLRRKFIDIDLGQIKPVYLSELSHYNHVLKQR
                     NSYLKSAQQIDAAFLAVLDEQLAGYGARVMEHRIDFINALEKEANTHHQAISNGLESL
                     SLSYQSSVVFDKKTNIYQQFLHQLEKNHQKDFFRKNTSVGPHRDNLAFYINGMNANFA
                     SQGQHRSLILSLKMAEVSLMKALTGDNPILLLDDVMSELDNTRQTKLLETVIKENVQT
                     FITTTSLDHLSQLPEGIRIFHVTKGTVQVDSDIH"
     gene            complement(1649..2512)
                     /locus_tag="VC122_001680"
     CDS             complement(1649..2512)
                     /locus_tag="VC122_001680"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019418724.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GRP family sugar transporter"
                     /protein_id="HEP5641582.1"
                     /translation="MEGIFYALIPMFTWGSIGFVSNKIGGKPSQQTLGMTFGALLFSL
                     AVWLIVRPEMTLQLWLFGILGGFIWSIGQTGQFHAMQYMGVSVANPLSSGSQLVLGSL
                     IGVLVFHEWTRPMQFVVGSLALLLLIIGFYFSSKQDDANAQVNHLHNFSKGFRALTYS
                     TIGYVMYAVLFNNIMKFEVLSVILPMAVGMVLGAITFMSFKISIDQYVIKNSVVGLLW
                     GIGNIFMLLAASKAGLAIAFSFSQLGAIISIVGGILFLGETKTKKEMRWVVTGIICFI
                     VGAILLGVVKS"
BASE COUNT          886 a          495 c          421 g          721 t
ORIGIN      
        1 tcttattcag ctattattat ttatttctgt tttatgctat aatgacttga atgacaaaat
       61 ggagatgaca atgatataca aattatttac agaatttatc accctacaag cccttcttaa
      121 agaattagga attatccaaa gtggtggcgc tatcaaggga tttctcgctg aaacaaccgt
      181 tctatttaat ggtgaagatg agaaacgtcg aggcaaaaaa atcagaatag gggataagat
      241 ttctctacct gaccaagact taattatcac tattgttgag cctagtcaag aagaaaaaga
      301 acaatttgcc gaagaaatag ctgaaaagac acgtgtagcg gctctagtga aacaaatgaa
      361 ccaagctaac aaaaagactt cttctaaaca taaaaatcgt caatctacta ccaaaaaatc
      421 actaagagca actaagaaaa ctaaaggtaa accaactgca cctgtccgct tcccaggtat
      481 ctagtatgtg gattaaagag ttagagttaa aacattatcg caattatgac cacctgcttg
      541 cctcattttc gtcgggttta aatgtcttta tcggtaataa tgctcaagga aaaaccaatt
      601 ttctcgaggc aatctatttt ctatcactta ctagaagtca tcgcacgagg gctgataaag
      661 aattgattca ttttgatcac tcaactgttt ctcttactgg taagattcag cgtattagtg
      721 gtactgttga tttagaaatc aatctatcag ataaaggacg tgtgacaaag attaatgcgc
      781 taaagcaagc aaaattatct gattatattg gaacgatgat ggttgtgctt tttgcaccag
      841 aagatttgca actagttaag ggagcaccta gccttcgtcg caaatttatc gacattgacc
      901 ttggtcaaat taagcctgtt tacttatctg agttatccca ttataaccac gtgcttaaac
      961 agcgtaatag ctatttaaaa agtgcccaac aaattgatgc cgcttttcta gctgttctag
     1021 atgagcaatt agctggctat ggagcccgtg tcatggaaca tcgtattgat tttatcaatg
     1081 cgttagagaa agaagcaaat actcatcacc aagccatctc aaatggttta gaaagtctct
     1141 ctctctccta tcagtcgtca gttgtctttg acaaaaaaac taatatttac caacaatttt
     1201 tacaccaact tgaaaaaaat catcagaaag atttcttccg taaaaacact agcgttggtc
     1261 cacatcgcga taacttagcc ttttacatta acgggatgaa tgctaacttt gctagtcaag
     1321 gtcagcaccg cagccttatt ctttccttaa aaatggctga agttagttta atgaaggcct
     1381 tgactggaga taaccccatt cttttactag acgatgtcat gagcgagcta gacaatacac
     1441 gacaaacaaa gttacttgaa acagtaatta aagaaaatgt ccaaaccttt atcacaacaa
     1501 caagtttgga tcacctgtca cagttgccag aaggtatccg tatttttcac gtaacaaaag
     1561 gaaccgttca agttgattct gatattcact aataacatca aaaaaagggt tcagttaaac
     1621 tgagcctttt tttgatatta tgtggcgatt aagatttgac cacacctaat aaaatagcac
     1681 ctacgataaa acaaataatt cctgtgacaa cccaacgcat ttcttttttg gttttggttt
     1741 cgcctaggaa taaaatgcct ccaacaatcg aaatgatagc acctaattgt gagaaactaa
     1801 aggcaatagc aagtcctgct tttgatgcag ctaaaagcat gaaaatattc ccaatgcccc
     1861 aaagtaatcc aacaacgcta tttttaatca catactggtc aatggaaatt ttaaatgaca
     1921 tgaaagttat agctcctaaa accataccta ctgccatggg caaaatgact gacaaaactt
     1981 caaatttcat gatgttatta aataatacag catacatcac gtaaccaatt gttgaataag
     2041 tgagcgccct aaatcctttt gaaaaattat gaaggtgatt gacttgtgca ttagcatcat
     2101 cttgtttact agaaaagtaa aaaccaataa tcagaagtaa tagtgctaga cttcctacta
     2161 caaattgcat aggtcttgtc cattcatgaa aaacgaggac accaatcaaa cttcccagaa
     2221 caagttgaga gccacttgac aaaggattgg caacggagac tcccatgtat tgcatggcat
     2281 gaaattgacc agtttgacca attgaccaga taaaaccacc caatattcca aaaagccaaa
     2341 gttgcagtgt catctcaggt ctaacaatta accaaacagc taatgaaaat aataaagccc
     2401 caaaagtcat tcctagagtt tgttgcgaag gttttcctcc aattttgtta ctaacaaatc
     2461 caatgcttcc ccatgtaaac atgggaataa gtgcataaaa aataccttcc aaaatgttat
     2521 ctc
//
LOCUS       DATXIG010000104         2415 bp    DNA     linear   BCT 28-DEC-2023
DEFINITION  TPA_asm: Streptococcus pyogenes strain MGAS11113
            SAMN08555799-rid19682583.denovo.104, whole genome shotgun sequence.
ACCESSION   DATXIG010000104 DATXIG010000000
VERSION     DATXIG010000104.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN08555799
            Sequence Read Archive: SRR6740495
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pyogenes
  ORGANISM  Streptococcus pyogenes
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 2415)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2415)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-OCT-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-OCT-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001918058.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001918058.2
            Genome Coverage       :: 368x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/12/2023 11:23:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 1,806
            CDSs (total)                      :: 1,769
            Genes (coding)                    :: 1,722
            CDSs (with protein)               :: 1,722
            Genes (RNA)                       :: 37
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1, 1 (5S, 23S)
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 47
            CDSs (without protein)            :: 47
            Pseudo Genes (ambiguous residues) :: 0 of 47
            Pseudo Genes (frameshifted)       :: 30 of 47
            Pseudo Genes (incomplete)         :: 20 of 47
            Pseudo Genes (internal stop)      :: 9 of 47
            Pseudo Genes (multiple problems)  :: 11 of 47
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2415
                     /organism="Streptococcus pyogenes"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN08555799-rid19682583.denovo.104"
                     /strain="MGAS11113"
                     /serotype="Emm28"
                     /isolation_source="normally sterile site"
                     /host="Homo sapiens"
                     /db_xref="taxon:1314"
                     /country="Finland"
                     /collection_date="2002"
                     /collected_by="National Public Health Laboratory"
     gene            <1..236
                     /locus_tag="VC122_001681"
     CDS             <1..236
                     /locus_tag="VC122_001681"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000491790.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="sensor histidine kinase"
                     /protein_id="HEP5641583.1"
                     /translation="TTDKNLLISVIDNGPGITDEEKKKIFDRFYRVDKARTRQTGGFG
                     LGLALAQQIVMSLKGNITVKDNDPKGSIFEVKL"
     gene            complement(361..609)
                     /locus_tag="VC122_001682"
     CDS             complement(361..609)
                     /locus_tag="VC122_001682"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008808726.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S20"
                     /protein_id="HEP5641584.1"
                     /translation="MEVKTLANIKSAIKRAELNVKANEKNSAQKSAMRTAIKAFEANP
                     SEELFRAASSSIDKAESKGLIHKNKASRDKARLAAKLG"
     gene            complement(663..1583)
                     /locus_tag="VC122_001683"
     CDS             complement(663..1583)
                     /locus_tag="VC122_001683"
                     /EC_number="2.7.1.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003058858.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I pantothenate kinase"
                     /protein_id="HEP5641585.1"
                     /translation="MSNEFINFEKISRESWKTLHQKAKALLTQEELKSITSLNDNISI
                     NDVIDIYLPLINLIQVYKIAQENLSFSKSLFLKKDIQLRPFIIGISGSVAVGKSTTSR
                     LLQLLLSRTHPNSQVELVTTDGFLYPNQFLIEQGLLNRKGFPESYNMELLLDFLDSIK
                     NGQTAFAPVYSHDIYDIIPNQKQSFNNPDFLIVEGINVFQNQQNNRLYMSDYFDFSIY
                     IDADSSHIETWYIERFLSILKLAKRDPHNYYAQYAQLPRSEAIAFARNVWKTVNLENL
                     EKFIEPTRNRAELILHKSADHKIDEIYLKK"
     gene            1818..>2415
                     /locus_tag="VC122_001684"
     CDS             1818..>2415
                     /locus_tag="VC122_001684"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011106780.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HEP5641586.1"
                     /translation="MKKYLKKIGKMMTKMYYDENPDSLHDIHEVKVELLNHPFTFLTD
                     SGVFSKKMVDFGSQVLLETLNFKENERVLDLGCGYGPLGISLAKVQRVDATLVDINNR
                     ALDLARKNATNNQVAVTIFQSNIYENISGHFEHIISNPPIRAGKRVVHSIIEKSIDFL
                     VVNGDLTIVIQKKQGAPSAKAKMATIFGNVEILRKDKGY"
BASE COUNT          814 a          337 c          446 g          818 t
ORIGIN      
        1 aaacaacgga caaaaattta ttaatttctg ttattgataa tggtccaggg ataacagatg
       61 aagaaaagaa aaagattttt gatcgttttt atcgagttga caaagctaga acacggcaaa
      121 caggtggatt tggcttgggg ttggctttag ctcagcaaat cgtgatgtct ttaaaaggaa
      181 atattacagt aaaggataat gatcctaaag gtagtatttt tgaagtcaaa ttataagcta
      241 aaaagggcga ccaataaggt cagccctttt aaagttattt ttaactgtca acgctttatt
      301 gtattttttg agtttttacc gtataaaaaa actccagctg gagttttcat tggatattta
      361 ttagccaagt tttgcagcaa ggcgtgcttt atcgcggctt gctttgtttt tgtgaatcaa
      421 accttttgat tcagctttat cgatgcttga agaagcagcg cggaaaagct cttcagatgg
      481 gttagcttca aatgctttga tagcagtacg catagctgat ttttgtgctg agtttttttc
      541 gtttgcttta acgttaagtt cagcgcgttt aatagctgat ttaatatttg ccaatgtttt
      601 cacctccaaa ttaatctaac tacattatta tatagaaaat atatgcgttt ggcaaccctt
      661 tatcactttt tgaggtaaat ttcatcaatt ttatgatcag cacttttatg taaaattaat
      721 tcagcacggt tacgtgtagg ttcaatgaat ttttcaagat tttctaaatt aacggttttc
      781 caaacgttgc gcgcaaatgc tatagcctca gatcgaggta attgtgcata ttgggcataa
      841 taattatgag ggtcacgttt tgctaatttt aaaatgctta gaaaccgttc aatgtaccag
      901 gtttcgatgt gactgctgtc tgcatcaata taaatagaaa aatcaaagta atcactcata
      961 tataaacgat tgttttgttg attttgaaaa acattaatac cttcgacgat aaggaagtca
     1021 gggttattaa atgattgttt ttgatttggg ataatatcgt aaatatcatg agaatagacg
     1081 ggtgcaaaag ccgtttgacc atttttgatg gaatctagaa aatctaagag aagttccatg
     1141 ttataagatt ctggaaatcc tttacgattt aacagtcctt gttcaataag aaactgatta
     1201 ggatataaaa aaccatcagt cgttaccagt tcgacttgac tattggggtg cgttcgtgat
     1261 aataaaagtt gtaaaagacg actagttgtt gatttaccaa cagctactga accagaaatt
     1321 ccaataataa aaggccttaa ttgaatgtct ttttttagaa aaaggctttt agaaaaagaa
     1381 agattttctt gcgcaatttt gtaaacctgg atcagattaa ttaatggaag gtagatatca
     1441 atgacatcgt taatactgat gttatcattt aagctagtaa tacttttaag ttcctcttgt
     1501 gtgagaagag cttttgcttt ttgatgtaat gttttccaag actctctaga aattttttca
     1561 aagttgataa attcatttga catggtagct cctaatcatt tgacagtgct tacattatac
     1621 cattttagtt aaaaaataaa taatttttcg ttagtattta cacgaaaatt aatacgatta
     1681 actcaaaaaa gccacacatt cctagtacaa ttatcctgct atattctaac aaatagttaa
     1741 agttcggttt ttatttgtaa tatagataaa attgttaaaa aacaagcgtc ttgtaaacca
     1801 tttcaaagta tgatagaatg aaaaagtacc ttaaaaagat aggaaaaatg atgacaaaga
     1861 tgtattatga tgaaaatcca gatagtctac acgatattca tgaagtaaaa gtagagctgc
     1921 ttaatcatcc ctttactttt ttgacggatt caggtgtctt ttcaaagaaa atggttgatt
     1981 tcggtagtca agtattatta gagacactaa atttcaagga gaacgaacgt gtactggact
     2041 taggttgcgg ctatggtcct cttgggatta gtctagccaa ggttcaaagg gtagacgcaa
     2101 ccttagtgga catcaataat cgtgctcttg atttagctag aaaaaatgca actaataacc
     2161 aagtggcagt aactattttt caatcaaata tttatgaaaa tattagtggc cattttgagc
     2221 atattattag taatcctcct attagagcag gaaagagagt agttcatagt attattgaaa
     2281 aaagcataga ttttttagtg gttaatggtg acttaacgat tgtgattcaa aaaaaacaag
     2341 gagctcctag cgctaaagca aaaatggcaa ctatttttgg taatgttgag atattaagga
     2401 aagataaagg ttatt
//
LOCUS       DATXIG010000105         2293 bp    DNA     linear   BCT 28-DEC-2023
DEFINITION  TPA_asm: Streptococcus pyogenes strain MGAS11113
            SAMN08555799-rid19682583.denovo.105, whole genome shotgun sequence.
ACCESSION   DATXIG010000105 DATXIG010000000
VERSION     DATXIG010000105.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN08555799
            Sequence Read Archive: SRR6740495
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pyogenes
  ORGANISM  Streptococcus pyogenes
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 2293)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2293)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-OCT-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-OCT-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001918058.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001918058.2
            Genome Coverage       :: 368x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/12/2023 11:23:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 1,806
            CDSs (total)                      :: 1,769
            Genes (coding)                    :: 1,722
            CDSs (with protein)               :: 1,722
            Genes (RNA)                       :: 37
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1, 1 (5S, 23S)
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 47
            CDSs (without protein)            :: 47
            Pseudo Genes (ambiguous residues) :: 0 of 47
            Pseudo Genes (frameshifted)       :: 30 of 47
            Pseudo Genes (incomplete)         :: 20 of 47
            Pseudo Genes (internal stop)      :: 9 of 47
            Pseudo Genes (multiple problems)  :: 11 of 47
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2293
                     /organism="Streptococcus pyogenes"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN08555799-rid19682583.denovo.105"
                     /strain="MGAS11113"
                     /serotype="Emm28"
                     /isolation_source="normally sterile site"
                     /host="Homo sapiens"
                     /db_xref="taxon:1314"
                     /country="Finland"
                     /collection_date="2002"
                     /collected_by="National Public Health Laboratory"
     gene            1..1118
                     /gene="hasA"
                     /locus_tag="VC122_001685"
                     /pseudo
     CDS             1..1118
                     /gene="hasA"
                     /locus_tag="VC122_001685"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011018340.1"
                     /note="frameshifted; incomplete; missing N-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=2
                     /transl_table=11
                     /product="hyaluronan synthase HasA"
     gene            1154..>2293
                     /gene="hasB"
                     /locus_tag="VC122_001686"
     CDS             1154..>2293
                     /gene="hasB"
                     /locus_tag="VC122_001686"
                     /EC_number="1.1.1.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002992300.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-glucose 6-dehydrogenase HasB"
                     /protein_id="HEP5641587.1"
                     /translation="MKIAVAGSGYVGLSLGVLLSLQNEVTIVDILPSKVDKINNGLSP
                     IQDEYIEDYLKSKQLSIKATLDSKAAYKEAELVIIATPTNYNSRINYFDTQHVETVIK
                     EVLSVNSHATLIIKSTIPIGFITEMRQKFQTDRIIFSPEFLRESKALYDNLYPSRIIV
                     SCEENDSPKVKADAEKFALLLKSAAKKNNVPVLIMGASEAEAVKLFDNTYLALRVAYF
                     NELDTYAESRKLNSHMIIQGISHDDRIGMHYNNPSFGYGGYCLPKDTKQLLANYNNIP
                     QTLIEAIVSSNNVRKSYIAKQIINVLEERESPVKVVGVYRLIMKSNSDNFRESAIKDV
                     IDILKSKDIKIIIYEPMLNKLESEDQSVLVNDLENFKKQANIIVTN"
BASE COUNT          791 a          365 c          386 g          751 t
ORIGIN      
        1 tggattatct ttcctttatg agccatttaa aggaaatcca catgactata aagttgctgc
       61 cgtaattcct tcttaataat gaagatgccg agtcattatt agaaactctt aaaagtgtgt
      121 tagcacagac ctatccgtta tcagaaattt atattgttga tgatgggagt tcaaacacag
      181 atgcaataca attaattgaa gagtatgtaa atagagaagt ggatatttgt cgaaacgtta
      241 tcgttcaccg ttcccttgtc aataaaggaa aacgccatgc tcaagcgtgg gcatttgaaa
      301 gatctgacgc tgacgttttt ttaaccgtag attcagatac ttatatctat ccaaatgcct
      361 tagaagaact cctaaaaagc ttcaatgata agacagttta tgctgcaaca ggacatttga
      421 atgctagaaa cagacaaact aatctattaa cgcgacttac agatatccgt tacgataatg
      481 cctttggggt ggagcgtgct gctcaatcat taacaggtaa tattttagtt tgctcaggac
      541 cattgagtat ttatcgacgt gaagtgatta ttcctaactt agagcgctat aaaaatcaaa
      601 cattcctagg tttacctgtt agcattgggg atgatcgatg tttaacaaat tatgctattg
      661 atttaggacg cactgtctac caatcaacag ctagatgtga tactgatgta cctttccaat
      721 taaaaagtta tttaaagcaa caaaatcgat ggaataaatc tttttttaga gaatctatta
      781 tttctgttaa aaaaattctt tctaatccca tcgttgcctt atggactatt ttcgaagtcg
      841 ttatgtttat gatgttgatt gtcgcaattg ggaatctttt gtttaatcaa gctattcaat
      901 tagaccttat taaacttttt gcctttttat ccatcatctt tatcgttgct ttatgtcgta
      961 atgttcatta tatggtcaaa catcctgcta gttttttgtt atctcctctg tatggaatat
     1021 tacacttgtt tgtcttacag cccctaaaac tttattcttt atgcaccatt aaaaatacgg
     1081 aatggggaac acgtaaaaag gtcactattt ttaaataata tatgcatcga gtagttagag
     1141 aaggagtaat tttatgaaaa tagcagttgc tggatcagga tatgttggat tatcactagg
     1201 agttctttta tcacttcaaa acgaagtcac tattgttgat attcttcctt ctaaagttga
     1261 taagattaat aatggcttat caccaattca agatgaatat attgaagatt acttaaaaag
     1321 taagcaatta tctattaaag caactttaga tagcaaagca gcttataaag aagcggaact
     1381 ggtcattatt gccacaccta caaattacaa cagtagaatt aattattttg atacacagca
     1441 tgttgaaaca gttatcaaag aggtactaag cgttaatagc catgcaactc ttatcatcaa
     1501 atcaacaatt ccaataggtt tcattactga aatgagacag aaattccaaa ctgatcgtat
     1561 tatcttcagc cctgaatttt taagagagtc taaagcttta tatgacaact tatatccaag
     1621 ccgaattatt gtttcttgtg aagaaaacga ttctccaaaa gtaaaggcag acgcagaaaa
     1681 atttgcactt ttattaaagt ctgcagctaa aaaaaataat gtaccagtac ttattatggg
     1741 agcttcagaa gctgaagcag taaaactatt tgacaatact tatttagcgt taagggtagc
     1801 ttattttaat gagttagaca cttacgcaga atcgagaaaa ttaaatagtc acatgattat
     1861 tcaaggaatt tctcatgatg atcgaatagg aatgcattat aataacccat catttggtta
     1921 tggaggttat tgtctaccta aagatacgaa gcaattattg gcaaattaca ataatattcc
     1981 ccaaacgcta attgaagcta tcgtttcatc aaataatgtg cgcaagtcct atattgctaa
     2041 gcaaattatc aacgtcttag aagagcggga gtccccagta aaagtagtcg gggtttaccg
     2101 tttaattatg aaaagtaact cagataattt tagagaaagt gctatcaaag atgttattga
     2161 cattcttaaa agtaaagaca ttaagataat tatttatgag ccaatgttaa acaaacttga
     2221 atctgaagat caatctgtac ttgtaaatga tttagagaat ttcaagaaac aagcaaatat
     2281 tatcgtaact aat
//
LOCUS       DATXIG010000106         2231 bp    DNA     linear   BCT 28-DEC-2023
DEFINITION  TPA_asm: Streptococcus pyogenes strain MGAS11113
            SAMN08555799-rid19682583.denovo.106, whole genome shotgun sequence.
ACCESSION   DATXIG010000106 DATXIG010000000
VERSION     DATXIG010000106.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN08555799
            Sequence Read Archive: SRR6740495
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pyogenes
  ORGANISM  Streptococcus pyogenes
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 2231)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2231)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-OCT-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-OCT-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001918058.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001918058.2
            Genome Coverage       :: 368x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/12/2023 11:23:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 1,806
            CDSs (total)                      :: 1,769
            Genes (coding)                    :: 1,722
            CDSs (with protein)               :: 1,722
            Genes (RNA)                       :: 37
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1, 1 (5S, 23S)
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 47
            CDSs (without protein)            :: 47
            Pseudo Genes (ambiguous residues) :: 0 of 47
            Pseudo Genes (frameshifted)       :: 30 of 47
            Pseudo Genes (incomplete)         :: 20 of 47
            Pseudo Genes (internal stop)      :: 9 of 47
            Pseudo Genes (multiple problems)  :: 11 of 47
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2231
                     /organism="Streptococcus pyogenes"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN08555799-rid19682583.denovo.106"
                     /strain="MGAS11113"
                     /serotype="Emm28"
                     /isolation_source="normally sterile site"
                     /host="Homo sapiens"
                     /db_xref="taxon:1314"
                     /country="Finland"
                     /collection_date="2002"
                     /collected_by="National Public Health Laboratory"
     gene            <1..165
                     /locus_tag="VC122_001687"
     CDS             <1..165
                     /locus_tag="VC122_001687"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019213953.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEP5641588.1"
                     /translation="NKELGIDLSIEIDKLIKQGEKPSVIAQIKKFQDQVNKDNEYRES
                     MKNKKMDQER"
     gene            239..412
                     /locus_tag="VC122_001688"
     CDS             239..412
                     /locus_tag="VC122_001688"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004829396.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEP5641589.1"
                     /translation="MNKRQELIDELIKADQDGTYKTYKSTEEIKAMNNEEIQIIYSNM
                     KNYLSDKRTHTNC"
     gene            596..1123
                     /locus_tag="VC122_001689"
     CDS             596..1123
                     /locus_tag="VC122_001689"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008902124.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PcfB family protein"
                     /protein_id="HEP5641590.1"
                     /translation="MINEEISKEAGQAAQTIISYTIKAAKESINLEKEIRKKMNDTLE
                     KANGNLKSLMGDEIKIKDLYKKGQLENISIDQSNLKDLKKELNKLGVSFSVMKNKESQ
                     NYEVFFQAKDIKVMEYAFKQVIAKENKKEKESILKQIKKYKDLSKNKNKTKEKVKRKV
                     KEKVKPNKKDMTREI"
     gene            1249..1977
                     /locus_tag="VC122_001690"
     CDS             1249..1977
                     /locus_tag="VC122_001690"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011284912.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage replisome organizer N-terminal
                     domain-containing protein"
                     /protein_id="HEP5641591.1"
                     /translation="MATNKRYYWIKLKEEFFTDKRIKRLRRISGGDTYTIIYLKLLLL
                     SLKDEGKLYYDGVESDFIKELALTIDETDDDVMVTVNYLINQGLLEVVTENDEYYLTE
                     IPNLIGSETAWAEKKRRYRQNKQRTLSLMSPTHVRQEIEIEKDIEIDKEREGEIDKKS
                     TPIFYGEFKNVRLSKEEYKNLKEKLNSHTEIMINKLSRYMESSGKTYQNHYATILNWY
                     DKDKDKLRQKSLNKKMNYDVGESL"
BASE COUNT         1102 a          308 c          369 g          452 t
ORIGIN      
        1 aataaagaac ttggcataga cttatccata gaaatagata agctaataaa acaaggagaa
       61 aaaccaagtg taatagcaca gattaaaaaa ttccaagacc aagtcaataa agataatgaa
      121 tacagagaaa gtatgaaaaa caagaaaatg gatcaagaaa gataagacct ggtaaaaata
      181 tcttatccaa gctataatat gaccaagaaa ataaagaccg ttctaggagg taatagagat
      241 gaataaaagg caagagctaa tagatgaact cataaaagca gatcaagatg gaacatacaa
      301 aacctacaaa agcacagaag aaataaaagc aatgaacaat gaagaaatac agattatata
      361 ttccaacatg aaaaactatc tatcagacaa aagaacacac acaaactgct gaaggtggaa
      421 aggtattgta agctatttaa aagtataaaa ggtacaaaca cctaaacaag atatgaaaac
      481 atcaaaataa agcattccac caccaaaaac aataaattaa aaacaagcaa agggaagtat
      541 agaaaaatta aaacctatac ttcccttttt ttaattcaaa caaaggagag aaaacatgat
      601 aaacgaagaa ataagcaaag aagcaggtca agcagcacaa accataatat catacacaat
      661 aaaggcagca aaagaatcaa tcaacttaga aaaagaaata agaaaaaaga tgaatgatac
      721 tttagaaaaa gcaaatggaa acctaaaaag tcttatgggc gatgaaataa aaataaaaga
      781 cctctacaaa aaaggacaac tagaaaacat aagcatagat caaagcaacc tcaaagactt
      841 aaaaaaagaa ctaaacaaac ttggagtaag cttttcagta atgaaaaaca aagaaagcca
      901 aaactatgaa gtatttttcc aagccaaaga cataaaagta atggaatatg cctttaagca
      961 agtcatagcc aaagaaaata aaaaagaaaa agaaagtatc ctaaaacaaa taaagaaata
     1021 caaagaccta tccaagaaca agaataagac gaaagagaaa gtaaaaagga aagtaaaaga
     1081 aaaagtaaaa ccaaacaaaa aagatatgac cagagaaatc taagggagta acaaaaagtt
     1141 acacccttaa ataaccataa taacaagact tacgaaaagc agaagtccag aattccgaaa
     1201 atcggaaatc aagaatttcg attatcgaaa atcaagaaag gagcagatat ggcaacaaac
     1261 aaaagatatt attggataaa actaaaagaa gaatttttca cagacaaaag gataaaaaga
     1321 ctaaggagaa tatcgggagg agatacctac acgataatct acctaaaact tttattacta
     1381 agtctaaaag atgagggcaa actctattat gacggagtag aaagtgattt tataaaagag
     1441 ttagcactaa ccatagatga aacagacgat gatgtaatgg tcacagtaaa ttatttaatc
     1501 aatcaaggct tactagaagt tgtaacagaa aatgatgaat actatttaac tgaaatacca
     1561 aacttaatcg gatcagaaac agcatgggca gagaaaaaaa gaagatacag acaaaataaa
     1621 cagaggacat tgtccttaat gagtccaacc catgtccgac aagagataga gatagagaaa
     1681 gatatagaga tagataaaga gagagaggga gagatagata aaaagtccac ccccattttt
     1741 tatggagaat tcaaaaatgt aaggttaagc aaagaagaat ataaaaatct taaagaaaaa
     1801 ctaaactcac acacagaaat aatgataaat aaactatcaa gatacatgga aagcagtggt
     1861 aagacctatc aaaaccacta tgcgacaatc ctaaattggt atgacaaaga taaagacaaa
     1921 ctaagacaga aaagtttaaa taaaaaaatg aattatgatg taggagaatc tttatagata
     1981 aaaagaggtg gaaagccact attaaagact ttaagtctaa aaaggtatca aaagtatgcc
     2041 agagataata aaaatgtaaa tatgacactt aaaaggcgat tagaaaatta aagaaatgct
     2101 agaagatttt tagatgacag ttaccaagag ggtctaattt tcactgctac tcatgcagtg
     2161 ggaattggac ctttttacat aagtaaattc tattcaaaat tagttaccaa gaaagacttc
     2221 ttttcactgt t
//
LOCUS       DATXIG010000107         2039 bp    DNA     linear   BCT 28-DEC-2023
DEFINITION  TPA_asm: Streptococcus pyogenes strain MGAS11113
            SAMN08555799-rid19682583.denovo.107, whole genome shotgun sequence.
ACCESSION   DATXIG010000107 DATXIG010000000
VERSION     DATXIG010000107.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN08555799
            Sequence Read Archive: SRR6740495
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pyogenes
  ORGANISM  Streptococcus pyogenes
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 2039)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2039)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-OCT-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-OCT-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001918058.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001918058.2
            Genome Coverage       :: 368x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/12/2023 11:23:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 1,806
            CDSs (total)                      :: 1,769
            Genes (coding)                    :: 1,722
            CDSs (with protein)               :: 1,722
            Genes (RNA)                       :: 37
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1, 1 (5S, 23S)
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 47
            CDSs (without protein)            :: 47
            Pseudo Genes (ambiguous residues) :: 0 of 47
            Pseudo Genes (frameshifted)       :: 30 of 47
            Pseudo Genes (incomplete)         :: 20 of 47
            Pseudo Genes (internal stop)      :: 9 of 47
            Pseudo Genes (multiple problems)  :: 11 of 47
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2039
                     /organism="Streptococcus pyogenes"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN08555799-rid19682583.denovo.107"
                     /strain="MGAS11113"
                     /serotype="Emm28"
                     /isolation_source="normally sterile site"
                     /host="Homo sapiens"
                     /db_xref="taxon:1314"
                     /country="Finland"
                     /collection_date="2002"
                     /collected_by="National Public Health Laboratory"
     gene            <1..694
                     /locus_tag="VC122_001691"
     CDS             <1..694
                     /locus_tag="VC122_001691"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011888524.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEP5641592.1"
                     /translation="QVKKLKQALDKIIASIESTDISKMSREVSRINNSLQADNDYIKQ
                     KYFIEYDIFEKDILARTGLLEIDWENVSDDIEPLKIHRNFYESISEKTDSEVIEEIKN
                     LEDINFTDEKGNDLDDLTIIVPAQDEGFKRVFLGQREWYDIRIGKSKRNRIKYIAGYE
                     VAPESGVKYWAEVDKIVPSDNYVGYWKVIFKGEVKEYSEYKRLGNTWPPQNSRYVSKK
                     ALDEAKYLSDVF"
     gene            714..2006
                     /gene="purB"
                     /locus_tag="VC122_001692"
     CDS             714..2006
                     /gene="purB"
                     /locus_tag="VC122_001692"
                     /EC_number="4.3.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000572892.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenylosuccinate lyase"
                     /protein_id="HEP5641593.1"
                     /translation="MIERYSRPEMAAIWTEENKYRAWLEVEILADEAWAELGEIPKED
                     VAKIREKADFDIDRILEIEQETRHDVVAFTRAVSETLGEERKWVHYGLTSTDVVDTAY
                     GYLYKQANDIIRRDLENFTNIVADKAREHKMTIMMGRTHGVHAEPTTFGLKLATWYSE
                     MKRNIERFEHAAAGVEAGKISGAVGNFANIPPFVEEYVCDKLGIRPQEISTQVLPRDL
                     HAEYFAVLASIATSIERMATEIRGLQKSEQREVEEFFAKGQKGSSAMPHKRNPIGSEN
                     MTGLARVIRGHMVTAYENVSLWHERDISHSSAERIITPDTTILIDYMLNRFGNIVKNL
                     TVFPENMMRNMESTFGLIYSQRVMLKLIEKGMTREEAYDLVQPKTAYSWDNQVDFKPL
                     LEEDTKVTSCLTQEELDELFNPIYYTKRVDDIFKRLGI"
BASE COUNT          666 a          349 c          465 g          559 t
ORIGIN      
        1 acaagttaaa aaattaaagc aagcattgga taaaataata gcctctatag agtctacaga
       61 tatttctaaa atgtcacgag aagtgtcacg cataaataac tccttacaag ccgataatga
      121 ctacatcaaa caaaaatatt ttatagaata tgatattttt gaaaaggata tattagctag
      181 gacagggtta ttagaaattg attgggaaaa cgtttctgat gacatagagc ccttaaagat
      241 acatcgtaac ttttatgaat caattagtga aaaaacagat tctgaagtta tagaagaaat
      301 caaaaattta gaagatataa atttcactga tgaaaagggt aatgatttgg atgacttaac
      361 cattattgtt cctgcgcaag acgaaggatt taaaagagtc tttctaggtc aacgagaatg
      421 gtatgatata cggataggaa aatcgaaaag gaataggatt aaatatatcg caggatatga
      481 agttgcacca gagagtggtg taaagtactg ggctgaagtg gataaaatag ttccttcaga
      541 taactatgtt ggttactgga aagtaatttt taaaggagag gtgaaggaat attcagaata
      601 taaaagatta ggaaatactt ggccccctca aaacagtaga tacgtatcta aaaaggcttt
      661 agatgaagct aaatatttat cggatgtttt ttaattaaag gagaaaaaac aaaatgatcg
      721 aacgttattc acgccctgag atggcggcaa tttggacaga ggaaaataaa taccgtgctt
      781 ggttggaggt tgagattctg gctgacgagg catgggcaga gttgggtgag attcctaagg
      841 aggatgtggc taagattcgt gagaaggcgg attttgacat tgaccgcatt cttgagattg
      901 agcaggagac gcgtcacgat gtggtggctt tcacgcgtgc ggtttctgaa acgcttggtg
      961 aggagcgcaa gtgggtgcac tacggtttga cctcgactga cgtggtggac actgcctacg
     1021 gttacctcta caagcaagct aacgacatta tccgtcgcga tcttgagaat ttcaccaata
     1081 tcgtggcaga caaggcgcgt gagcacaaaa tgaccatcat gatgggtcgt acccacggtg
     1141 ttcacgccga gccaacgact ttcggtctta agttggcgac ttggtacagc gagatgaaac
     1201 gtaatattga gcgttttgaa catgctgctg caggtgtgga agctggtaag atttcaggtg
     1261 ccgttggtaa ctttgccaac atcccacctt ttgtggaaga atatgtctgt gacaaattag
     1321 gcattcgtcc gcaagaaatt tcaacacaag ttcttccacg tgaccttcac gcagaatact
     1381 ttgcagtgct tgcaagtatt gcaacttcta tcgaacgtat ggcgacagag attcgtggtc
     1441 tgcaaaaatc agaacaacgt gaagttgaag agttctttgc caaaggtcag aaaggtagct
     1501 ctgctatgcc tcacaaacgc aacccaatcg gttcagagaa catgacaggg ctagcgcgcg
     1561 tgattcgtgg tcacatggtg acagcttatg agaacgtgtc actttggcat gagcgtgata
     1621 tttcacactc atcagctgag cgtatcatca cacctgacac aactatcttg attgactaca
     1681 tgctcaaccg ctttggcaat atcgttaaga acttgactgt cttcccggaa aatatgatgc
     1741 gcaatatgga atcaactttt ggtttaattt atagtcaacg tgttatgctc aaattgattg
     1801 aaaaaggaat gacacgagaa gaagcttatg acttagttca acctaagaca gcttattcct
     1861 gggacaatca agtggatttc aaaccacttt tagaagaaga caccaaagtt acctcttgtc
     1921 ttacacaaga agaacttgat gaactattta atccgattta ttacacaaaa cgtgttgatg
     1981 atatttttaa gcgtttaggg atttaatata aaaaaaagaa ggtgattccc ttctttttt
//
LOCUS       DATXIG010000108         1950 bp    DNA     linear   BCT 28-DEC-2023
DEFINITION  TPA_asm: Streptococcus pyogenes strain MGAS11113
            SAMN08555799-rid19682583.denovo.108, whole genome shotgun sequence.
ACCESSION   DATXIG010000108 DATXIG010000000
VERSION     DATXIG010000108.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN08555799
            Sequence Read Archive: SRR6740495
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pyogenes
  ORGANISM  Streptococcus pyogenes
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 1950)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1950)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-OCT-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-OCT-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001918058.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001918058.2
            Genome Coverage       :: 368x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/12/2023 11:23:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 1,806
            CDSs (total)                      :: 1,769
            Genes (coding)                    :: 1,722
            CDSs (with protein)               :: 1,722
            Genes (RNA)                       :: 37
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1, 1 (5S, 23S)
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 47
            CDSs (without protein)            :: 47
            Pseudo Genes (ambiguous residues) :: 0 of 47
            Pseudo Genes (frameshifted)       :: 30 of 47
            Pseudo Genes (incomplete)         :: 20 of 47
            Pseudo Genes (internal stop)      :: 9 of 47
            Pseudo Genes (multiple problems)  :: 11 of 47
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1950
                     /organism="Streptococcus pyogenes"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN08555799-rid19682583.denovo.108"
                     /strain="MGAS11113"
                     /serotype="Emm28"
                     /isolation_source="normally sterile site"
                     /host="Homo sapiens"
                     /db_xref="taxon:1314"
                     /country="Finland"
                     /collection_date="2002"
                     /collected_by="National Public Health Laboratory"
     gene            109..588
                     /gene="rlmH"
                     /locus_tag="VC122_001693"
     CDS             109..588
                     /gene="rlmH"
                     /locus_tag="VC122_001693"
                     /EC_number="2.1.1.177"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002991465.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA
                     (pseudouridine(1915)-N(3))-methyltransferase RlmH"
                     /protein_id="HEP5641594.1"
                     /translation="MKVKLICVGKLKERYLKDGISEYQKRLSRFCQFEMIELTDERTP
                     DKASFADNQLIMSKEAQRIHKKIGERDFVIALAIEGKQFPSETFSELISGVTVKGYST
                     ITFIIGGSLGLDSIIKKRANMLMSFGLLTLPHQLMRLVLTEQIYRAFMITQGSPYHK"
     gene            complement(746..819)
                     /locus_tag="VC122_001694"
     tRNA            complement(746..819)
                     /locus_tag="VC122_001694"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(783..785),aa:Arg,seq:tct)
     gene            984..1758
                     /locus_tag="VC122_001695"
                     /pseudo
     CDS             984..1758
                     /locus_tag="VC122_001695"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001871253.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS30 family transposase"
BASE COUNT          685 a          336 c          351 g          578 t
ORIGIN      
        1 ctttttacta ataaagacct ttgtacaaag gtttttatta gtagttgaag aaaaataact
       61 tgtggataaa ttgtgtaaaa agtaatgttc ttgtgataca attaagctat gaaagtgaaa
      121 ctaatttgtg ttggaaaact aaaagaaaga taccttaaag atggtatttc agaatatcaa
      181 aaaagacttt cccgtttttg tcaattcgaa atgattgagt taactgatga aagaacacca
      241 gataaagcaa gttttgctga taatcaattg attatgagta aagaagctca gcgaatccat
      301 aaaaaaattg gggaacgtga ttttgtaatt gcattagcta ttgaaggaaa acagtttcct
      361 tcagagacat ttagtgaatt aatatcaggg gttactgtta agggatattc aactatcacg
      421 tttattattg gtgggagtct tggattagat tcaattatta aaaaaagagc taatatgcta
      481 atgagttttg gattgttaac cttacctcat cagttaatgc gtctagttct tactgagcaa
      541 atttatcgtg cttttatgat tacacaaggc agtccttatc ataagtaaga gagtgtgaac
      601 tggaataact aactcttacc actttattag ataatagttc ggtataatta aaagcaaata
      661 ttaaaaaaag acgtactaaa tcctatcttt tcaatacact aaggtaaata caaaaaaaag
      721 cactaaaaca gtgctttttt atctttgatc ccagcaggat tcgaacctgc gaccgttcgc
      781 ttagaaggcg aatgctctat ccagctgagc tatgagacct taaccacatc attttaacaa
      841 aaattatcta aaactgtcaa tatttatttt gaaaaaacta gaaaataaca taatatcccg
      901 cgttgtaaaa tgaagtgcaa caaaaaagac atgttcatct gatatactag aattccccaa
      961 ttcagtatgg aaagcagatg aacatgtcta atattaattc taccagaaag tcatcctatt
     1021 ctcatctttc agccacagaa cggggtgaaa ttgctgctta tcttaaaatg ggaaagaaac
     1081 ccgttgagat tgctcgactt ctggggcgtc actgttctac aatctgccga gaaataaaac
     1141 gtggttcagt agatcaggta aaggataaga atggaaaaca aaccttcttc aacgcctatt
     1201 tcgctgatag tgggcaacgt gtctattaaa ccaatcgatt tgcctaaggt ggttagtatc
     1261 agaaaacgtt ctaagaaagt catgaagacg aataagaaga ctttaggtaa atctatcgaa
     1321 gaacgtccag aatatattaa tgatcgttct gaatttgggc attgggagat tgatttggtt
     1381 ctcggcaaga aaaccaaaag tgaagctgtt atgttgactt tggtagagcg ccagacacgc
     1441 tatgcactag gagttaagct agaagacaag cagtcccaaa ccattaaccg tgctgtcacg
     1501 catctcatca gtcagtatcc tattgcatcc atcacagcgg ataatggttc tgagtttagt
     1561 ttactctcag acttaaaagc tgttgacgtt tactttgcac atccctattc ttcacatgag
     1621 agaggaacaa gcgagaactt caatggcctc ctcagagaat atgtccctaa aggagtctca
     1681 cttaatccac taacctctga agaacttgac aatgatatta ctgccatcaa tgagcgccta
     1741 agacgacttc ttcaatagca atcctcaaaa ttcctgtttg agctatcccg aacagcttaa
     1801 cctctggaac tctagttatc tatgaactcg ttgcacttga cttgacaact tgcggtttaa
     1861 aaaaaatcaa atttttttaa aaaaagtata gacaaaaaaa atcaatctgc tataatagta
     1921 agagtgttaa ggagatatcc tccaaaactt
//
LOCUS       DATXIG010000109         1936 bp    DNA     linear   BCT 28-DEC-2023
DEFINITION  TPA_asm: Streptococcus pyogenes strain MGAS11113
            SAMN08555799-rid19682583.denovo.109, whole genome shotgun sequence.
ACCESSION   DATXIG010000109 DATXIG010000000
VERSION     DATXIG010000109.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN08555799
            Sequence Read Archive: SRR6740495
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pyogenes
  ORGANISM  Streptococcus pyogenes
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 1936)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1936)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-OCT-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-OCT-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001918058.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001918058.2
            Genome Coverage       :: 368x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/12/2023 11:23:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 1,806
            CDSs (total)                      :: 1,769
            Genes (coding)                    :: 1,722
            CDSs (with protein)               :: 1,722
            Genes (RNA)                       :: 37
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1, 1 (5S, 23S)
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 47
            CDSs (without protein)            :: 47
            Pseudo Genes (ambiguous residues) :: 0 of 47
            Pseudo Genes (frameshifted)       :: 30 of 47
            Pseudo Genes (incomplete)         :: 20 of 47
            Pseudo Genes (internal stop)      :: 9 of 47
            Pseudo Genes (multiple problems)  :: 11 of 47
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1936
                     /organism="Streptococcus pyogenes"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN08555799-rid19682583.denovo.109"
                     /strain="MGAS11113"
                     /serotype="Emm28"
                     /isolation_source="normally sterile site"
                     /host="Homo sapiens"
                     /db_xref="taxon:1314"
                     /country="Finland"
                     /collection_date="2002"
                     /collected_by="National Public Health Laboratory"
     gene            791..1225
                     /locus_tag="VC122_001696"
     CDS             791..1225
                     /locus_tag="VC122_001696"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015057940.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HEP5641595.1"
                     /translation="MIDVSKKLKQLRIDRGIAKSRLITYLSITLRTYDRYEDGSRTPD
                     LVTLMKLSDFYQCSLDELVKGNTNDTLVDRALIVSYRDLKDKGLNDKLAHGLVKQVYD
                     TYEFEKIPQISDANRIKFIYKEDLVTLLESIVSKIQEEKKCD"
     gene            1383..1823
                     /locus_tag="VC122_001697"
     CDS             1383..1823
                     /locus_tag="VC122_001697"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015057939.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin family protein"
                     /protein_id="HEP5641596.1"
                     /translation="MEHRLSMHYFQVKKEIFGVLLLSPVILLIMWGYVSSNQDDGRLT
                     QKQYYETVVNQTVNLVFYKKSCPYCKVAKKEIATQAKDSQVITYYVDTSTGEGQKLVK
                     KYHVKYAPTIVSIRKGNIQSFLYARDRGNNIIVEKEKIKEVFSQ"
BASE COUNT          698 a          225 c          381 g          632 t
ORIGIN      
        1 aaaaaataaa acactaattt gaggaataga gttaaatttt agaacattga taattaaata
       61 aaaagtgcgt cagacaacac agctaagcta agttgaataa cacacataat aaaacataga
      121 cacttttatt atagcgtgaa attcttcaga agtaaagcaa gtgtagttta ctataccaag
      181 ttgtctgata acttttgaag tttgaatttt gcttgtttaa gcaaaaaaag ttagatagtt
      241 attcctacct agcttagttt tactataaaa attgatgcaa aatagttgtg gccatgatta
      301 tagtaattac tgtgtgttca actaaacaag taaagttcag acctcaaaag cacactaagg
      361 tttatgttag ttagcctgtg ttgagcaaaa aactaaaact gctactctgc aaggggtgtt
      421 gacaatgatt actgaaaacg tggtgaggcg gattaggtat ggtattgtgg gcggtgtgtt
      481 agactttgtc tagctaaagg ataaggtgga cttattcgac tgagtagctc atgttgagaa
      541 acatagtgaa aggagtgtgc gaagatacga gcatgcaatt taaattgaaa tatagtgaga
      601 atattttttt ctatgtcgac ttctttcata agtcgacttt tttgttaaaa aataaaaata
      661 gaaatatagt ttaggtatta tggggtatcg tcctttctct aagctatatt tttttatttt
      721 tggaaagggt attaagcata gtatcttttt taaaggtaaa aatttagaat gattgaaagg
      781 gagctgaata atgattgatg tatcaaaaaa actaaaacaa ctaagaattg atagagggat
      841 tgctaaatcg cggttgatca cttatttatc gataacttta agaacttatg acagatatga
      901 agatggtagt cgtactcctg atttagtaac gttaatgaaa ttatcggatt tttatcaatg
      961 ctcgcttgat gaattagtta aagggaacac aaatgatacg cttgttgata gggcattgat
     1021 tgtttcgtat agagatctta aagataaggg tttaaatgat aaattggctc atggtctagt
     1081 gaagcaggtc tatgatactt atgaatttga gaagattcct caaatatctg atgctaatag
     1141 aattaagttt atctataaag aagatttggt tactttactt gaaagtatcg tgtcaaaaat
     1201 acaagaggag aagaaatgtg actagctaaa agattttgga ggtaatgaat gttagtttat
     1261 gaatttatta aacaaaagat tttgtgtcat cagctaacca ggttgttacg aataggtact
     1321 gtaatgataa ctggactctc ctatagttta gataactgga aaaagtggag ggggattgaa
     1381 atatggagca tcgattgagt atgcactatt ttcaagtgaa aaaagaaatt tttggggtat
     1441 tattattgag tcccgttatt ttattgatta tgtggggcta tgtttctagc aatcaagatg
     1501 atggtcgact aacgcaaaag caatactatg aaacagtggt caaccaaacg gttaatttag
     1561 tgttttataa aaagtcatgt ccctattgca aagtagctaa aaaagaaatt gctactcaag
     1621 ctaaggatag tcaagttatt acttattatg ttgatactag tacgggagaa ggacaaaaat
     1681 tggttaaaaa ataccatgtt aagtacgcac ctacaatagt atcaattaga aaaggaaata
     1741 ttcaatcatt tttgtatgct cgagatagag gcaacaacat tatagtagaa aaagaaaaga
     1801 taaaagaagt atttagtcag taagctatga ctttatgtgt catagctttt ttatatttgt
     1861 tgaaaacaag ggtaaaatac agtaaatctc cattcttcat tctccatttt taaaccgaaa
     1921 agattttact cttgtt
//
LOCUS       DATXIG010000110         1721 bp    DNA     linear   BCT 28-DEC-2023
DEFINITION  TPA_asm: Streptococcus pyogenes strain MGAS11113
            SAMN08555799-rid19682583.denovo.110, whole genome shotgun sequence.
ACCESSION   DATXIG010000110 DATXIG010000000
VERSION     DATXIG010000110.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN08555799
            Sequence Read Archive: SRR6740495
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pyogenes
  ORGANISM  Streptococcus pyogenes
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 1721)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1721)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-OCT-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-OCT-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001918058.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001918058.2
            Genome Coverage       :: 368x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/12/2023 11:23:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 1,806
            CDSs (total)                      :: 1,769
            Genes (coding)                    :: 1,722
            CDSs (with protein)               :: 1,722
            Genes (RNA)                       :: 37
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1, 1 (5S, 23S)
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 47
            CDSs (without protein)            :: 47
            Pseudo Genes (ambiguous residues) :: 0 of 47
            Pseudo Genes (frameshifted)       :: 30 of 47
            Pseudo Genes (incomplete)         :: 20 of 47
            Pseudo Genes (internal stop)      :: 9 of 47
            Pseudo Genes (multiple problems)  :: 11 of 47
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1721
                     /organism="Streptococcus pyogenes"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN08555799-rid19682583.denovo.110"
                     /strain="MGAS11113"
                     /serotype="Emm28"
                     /isolation_source="normally sterile site"
                     /host="Homo sapiens"
                     /db_xref="taxon:1314"
                     /country="Finland"
                     /collection_date="2002"
                     /collected_by="National Public Health Laboratory"
     gene            73..807
                     /locus_tag="VC122_001698"
     CDS             73..807
                     /locus_tag="VC122_001698"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014407398.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="coenzyme F420-0:L-glutamate ligase"
                     /protein_id="HEP5641597.1"
                     /translation="MNLSIFGLKNIPYLKEGDSIEKLIEESIKTSGFFIEDNDVLCIA
                     SKVVSIAEGQVMSLNEIQVSDVAKEIHRNIPRKDPRIIEIMLNLVNRDLSRLDIKKNY
                     IGCRLENGLKLTSGGIDRKSVDEVFLLPNNPDASAKRISEYLKKSLGKNVAVVITDSD
                     GREDKRGATQVAIGIYGIHPLRKTEVIDSQGETIKFQEETLCDMIAACAGLVMGQRGT
                     GIPAVLIKGLDYKWSENTSIKEALNE"
     gene            800..>1721
                     /locus_tag="VC122_001699"
     CDS             800..>1721
                     /locus_tag="VC122_001699"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010921994.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dehydrogenase"
                     /protein_id="HEP5641598.1"
                     /translation="MSRNQDCKNILIIGFGKIGKIKAYKWLSRGYEVYVKDLKFSSMK
                     YLNTNSDVLDDLSRQYFTIEICTPTNHHLTLLKTVITRYVYSYISIEKPLCNRLEDLE
                     EMKSLVESHPHLAKKMFASEQYFFSKILEKLLQVDCFSLDKIKEITVNFSKDRIEDNE
                     NGRFLDKEILGYGIEIPHIIAVISYLGISLEEFKKGIFVNAIYIKDLEKHDYSIEILS
                     CINQTVIKIISNLGSFAIREGKIEGGDAKTVRRVTIDDKTILFDPHPKLERYKSELVT
                     GTNTFQIHDDMMDTYISLLEGNAIPEDCYIN"
BASE COUNT          642 a          209 c          353 g          517 t
ORIGIN      
        1 aaatagacaa aaaggcagaa gtagtgagga agtcttatct gacactaaat gagtatatta
       61 atggaggcta aaatgaattt gagtatattt ggtttaaaga atattcctta tttgaaagaa
      121 ggggatagta tagaaaagct tattgaagaa agcattaaaa cttcaggatt ttttatcgaa
      181 gacaatgatg tgctttgcat agcttctaaa gttgtatcta tcgcagaagg ccaggtgatg
      241 tctttaaacg agatacaagt gagtgatgta gcaaaagaaa tccacaggaa tattcctcgt
      301 aaagatccta gaataattga aattatgcta aatttagtca atagagatct gtcacgttta
      361 gatatcaaaa aaaattacat tggttgtcgc ttggaaaatg gtttaaagtt gacaagtggt
      421 ggaatcgata gaaaatcagt agatgaagta tttcttttac ctaataatcc ggatgcttct
      481 gcaaaaagaa tttcggaata tcttaaaaaa tcacttggaa aaaatgttgc agttgttata
      541 acagatagtg atggtagaga ggataaaaga ggtgctactc aagtagcaat tggtatttat
      601 ggtattcatc ctttgagaaa aacagaagta atagattcac aaggagagac aataaaattc
      661 caagaagaga ctttgtgtga tatgattgca gcttgtgctg gcttagtaat ggggcaacgt
      721 ggaactggaa ttccagctgt cctcattaag ggcttggatt ataaatggtc tgaaaatact
      781 agtattaagg aagcgttaaa tgagtagaaa tcaggactgt aagaatattc taatcattgg
      841 ttttggaaaa attgggaaaa ttaaagcgta caagtggcta tcacgcggat atgaggtata
      901 tgtcaaagat ttgaaattct ccagcatgaa ataccttaat acaaatagtg atgtgttgga
      961 tgatttatca cgacaatatt ttactattga gatttgtact ccgacaaatc accaccttac
     1021 gctattgaaa accgttataa caaggtatgt ctattcttat attagcattg aaaaaccact
     1081 gtgtaacagg ttagaagatt tagaagaaat gaagtcgcta gtagaaagtc acccacatct
     1141 agcaaaaaaa atgtttgcca gtgaacaata cttcttttcg aaaattttgg aaaaactgtt
     1201 acaagtagat tgtttctctc tagataaaat caaggaaata acagttaatt tttctaaaga
     1261 taggattgag gataacgaga atgggagatt tttagataaa gagatattgg gctatggaat
     1321 agagattcct catatcattg ctgtcatttc ttatttagga atttcattag aagaattcaa
     1381 aaaagggatc tttgtgaatg ctatttatat aaaagactta gagaaacacg attattcaat
     1441 agaaatatta tcttgtatta atcaaacagt gattaaaatt attagtaatt tgggaagttt
     1501 tgcaataaga gagggcaaga ttgaaggagg tgatgctaaa acagtaagaa gagttaccat
     1561 tgatgacaaa acaatactat ttgatccaca tcctaaactc gagaggtata agagtgaatt
     1621 ggtgactggg acaaatacgt ttcaaataca cgatgatatg atggacacgt atatcagttt
     1681 gttagaagga aatgctattc ctgaagattg ctatatcaat a
//
LOCUS       DATXIG010000111         1712 bp    DNA     linear   BCT 28-DEC-2023
DEFINITION  TPA_asm: Streptococcus pyogenes strain MGAS11113
            SAMN08555799-rid19682583.denovo.111, whole genome shotgun sequence.
ACCESSION   DATXIG010000111 DATXIG010000000
VERSION     DATXIG010000111.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN08555799
            Sequence Read Archive: SRR6740495
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pyogenes
  ORGANISM  Streptococcus pyogenes
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 1712)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1712)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-OCT-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-OCT-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001918058.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001918058.2
            Genome Coverage       :: 368x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/12/2023 11:23:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 1,806
            CDSs (total)                      :: 1,769
            Genes (coding)                    :: 1,722
            CDSs (with protein)               :: 1,722
            Genes (RNA)                       :: 37
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1, 1 (5S, 23S)
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 47
            CDSs (without protein)            :: 47
            Pseudo Genes (ambiguous residues) :: 0 of 47
            Pseudo Genes (frameshifted)       :: 30 of 47
            Pseudo Genes (incomplete)         :: 20 of 47
            Pseudo Genes (internal stop)      :: 9 of 47
            Pseudo Genes (multiple problems)  :: 11 of 47
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1712
                     /organism="Streptococcus pyogenes"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN08555799-rid19682583.denovo.111"
                     /strain="MGAS11113"
                     /serotype="Emm28"
                     /isolation_source="normally sterile site"
                     /host="Homo sapiens"
                     /db_xref="taxon:1314"
                     /country="Finland"
                     /collection_date="2002"
                     /collected_by="National Public Health Laboratory"
     gene            <1..282
                     /locus_tag="VC122_001700"
     CDS             <1..282
                     /locus_tag="VC122_001700"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011888686.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1642 domain-containing protein"
                     /protein_id="HEP5641599.1"
                     /translation="SIYDEKVEEDDFYYWMQKSKNPVRTLINMHQFGYEIEQEQLYTV
                     DLPNGQPLVRGKNTLYFSQNLTAENAKLTESEIRKDFEWAWQFAKEVTE"
     gene            731..1165
                     /locus_tag="VC122_001701"
     CDS             731..1165
                     /locus_tag="VC122_001701"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011284866.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ArpU family transcriptional regulator"
                     /protein_id="HEP5641600.1"
                     /translation="MTFFPEINIQKTKSNAKRKLREYPRWRRIANDVDTQKVTATYSF
                     EPRQPHGVPSKPVERLALNRVSAEQELDAIEQAVSMILEPERRRILYDKYLAPYKKAD
                     KVIYTELCMSESFYYDTLDIALLAFAELYREGVLLVEQGVFS"
BASE COUNT          554 a          314 c          368 g          476 t
ORIGIN      
        1 tcaatctatg atgaaaaagt tgaagaagat gatttttatt attggatgca aaaatcaaag
       61 aatccagttc gtactcttat taatatgcac caatttggct atgaaatcga gcaagagcag
      121 ctgtatacag ttgatttgcc aaacggtcag cctttagtac gcggtaaaaa cactttgtat
      181 tttagtcaaa atctaacagc cgaaaatgca aaactcactg aatcagaaat ccgaaaagac
      241 ttcgagtggg cttggcaatt tgcgaaagag gtgactgaat gactgaagag ttaggagtgt
      301 tatatagcga aaaatggcat aagtattatt tatataaaac ttgcaggtat atatcttatg
      361 ttgataatcc acatcaggct actaaatgca cccgcaaaca agcagaacag tttccacagt
      421 ttaaatgggt atcgctgaca aaattataac cccacgcaag cgctcaagag cctgcgatgg
      481 ctctgtgggg gtggaccgaa attaaaaaat agaaacgaga acctccttac accaaaacaa
      541 atctaaagcg ggttatcggt catccgtgat tatccaaggc gtcgctaatg ctttaacacg
      601 acatcgtgcg cctgtgtcaa aaaacaaagg aaagagaggg cttttctcca caaaacaaaa
      661 agacgtccac acggaacgcc cccttggtta aatttaagct taaataaatt ataccacatc
      721 gggggctttc atgacgtttt ttccagaaat taatatacaa aaaactaaat caaacgctaa
      781 gcgaaaacta agagagtatc cacgctggcg aaggatagct aatgacgtag atactcaaaa
      841 agttacagct acttactcat ttgagccaag gcaaccgcat ggggtcccta gtaaaccagt
      901 tgagagacta gcgctaaacc gtgtgtcagc agaacaagag ctggatgcga ttgagcaagc
      961 cgtcagtatg atactagagc cagagagacg caggattttg tatgacaaat atttagcgcc
     1021 ttataaaaag gcagataagg ttatttatac agaattgtgt atgtcagaga gcttttacta
     1081 tgatacactt gacattgctt tgttagcttt tgcagagctg tacagagagg gtgtgttgct
     1141 tgttgagcaa ggagttttta gctagttttt atacagtaat aagatagttt atacatattt
     1201 ttacatgtta ttatagtact atcaaaataa caagaagaga taacctttta atcactgact
     1261 attttattta gtcgccaact ttaactacga tcaaacttgt tattttatgg tatgtgggac
     1321 gtgcaggttc gaatcctgcc gtctctatca gcgtcagggg agacgcgacc ccacacccat
     1381 ttaaaggtag acacgatctg atgtgtgggg ttttatgatt aaccgcaagt aaaacaaggg
     1441 tcgcaacctt gcttgtggtt agtaggctta atggtcgttt atgcacgttc gattcgtgca
     1501 aagcctgtta ggaacatgaa ccgtgattgg aaaacggtgg aggtagcgcc ttgcttcggg
     1561 acgttagcta agtccgaaaa ctctttgcga agagccagaa gaagatgtgt cggttcgatt
     1621 ccgactgttc ctgttgcatt taatgcaaac tccatatttt tattaaaaaa gcctattata
     1681 cctattgtgt aagggctttt ttcgttgtat ta
//
LOCUS       DATXIG010000112         1659 bp    DNA     linear   BCT 28-DEC-2023
DEFINITION  TPA_asm: Streptococcus pyogenes strain MGAS11113
            SAMN08555799-rid19682583.denovo.112, whole genome shotgun sequence.
ACCESSION   DATXIG010000112 DATXIG010000000
VERSION     DATXIG010000112.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN08555799
            Sequence Read Archive: SRR6740495
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pyogenes
  ORGANISM  Streptococcus pyogenes
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 1659)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1659)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-OCT-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-OCT-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001918058.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001918058.2
            Genome Coverage       :: 368x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/12/2023 11:23:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 1,806
            CDSs (total)                      :: 1,769
            Genes (coding)                    :: 1,722
            CDSs (with protein)               :: 1,722
            Genes (RNA)                       :: 37
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1, 1 (5S, 23S)
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 47
            CDSs (without protein)            :: 47
            Pseudo Genes (ambiguous residues) :: 0 of 47
            Pseudo Genes (frameshifted)       :: 30 of 47
            Pseudo Genes (incomplete)         :: 20 of 47
            Pseudo Genes (internal stop)      :: 9 of 47
            Pseudo Genes (multiple problems)  :: 11 of 47
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1659
                     /organism="Streptococcus pyogenes"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN08555799-rid19682583.denovo.112"
                     /strain="MGAS11113"
                     /serotype="Emm28"
                     /isolation_source="normally sterile site"
                     /host="Homo sapiens"
                     /db_xref="taxon:1314"
                     /country="Finland"
                     /collection_date="2002"
                     /collected_by="National Public Health Laboratory"
     gene            complement(62..1543)
                     /gene="guaB"
                     /locus_tag="VC122_001702"
     CDS             complement(62..1543)
                     /gene="guaB"
                     /locus_tag="VC122_001702"
                     /EC_number="1.1.1.205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003085743.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IMP dehydrogenase"
                     /protein_id="HEP5641601.1"
                     /translation="MSNWDTKFLKKGYTFDDVLLIPAESHVLPNEVDLKTKLADNLTL
                     NIPIITAAMDTVTGSKMAIAIARAGGLGVIHKNMSITEQAEEVRKVKRSENGVIIDPF
                     FLTPEHKVSEAEELMQRYRISGVPIVETLANRKLVGIITNRDMRFISDYNAPISEHMT
                     SEHLVTAAVGTDLETAERILHEHRIEKLPLVDNSGRLSGLITIKDIEKVIEFPHAAKD
                     EFGRLLVAAAVGVTSDTFERAEALFEAGADAIVIDTAHGHSAGVLRKIAEIRAHFPNR
                     TLIAGNIATAEGARALYDAGVDVVKVGIGPGSICTTRVVAGVGVPQVTAIYDAAAVAR
                     EYGKTIIADGGIKYSGDIVKALAAGGNAVMLGSMFAGTDEAPGETEIYQGRKFKTYRG
                     MGSIAAMKKGSSDRYFQGSVNEANKLVPEGIEGRVAYKGAASDIVFQMLGGIRSGMGY
                     VGAGDIQELHENAQFVEMSGAGLIESHPHDVQITNEAPNYSVH"
BASE COUNT          522 a          381 c          289 g          467 t
ORIGIN      
        1 ttttaatata aaaaaagagc tggcacaata tgtcagccct taaagttact aaattaaatt
       61 atcagtgcac agaatagttt ggtgcttcat ttgtgatttg gacatcatgc ggatgacttt
      121 caatcaagcc tgcacctgac atttcaacaa attgagcatt ttcatgtaat tcttgaatgt
      181 cacctgcgcc aacatagccc atacctgagc ggataccacc taacatttgg aagacaatat
      241 ctgaagcagc tcctttataa gcaacacggc cttcaatacc ttctggaact agtttattag
      301 cttcatttac ggaaccttgg aagtaacgat cgcttgatcc tttcttcata gctgcaatgg
      361 aacccattcc acgataagtt ttgaacttac ggccttggta gatttcagtt tcacctggag
      421 cttcatcagt acctgcaaac attgatccaa gcataacagc attaccgccg gctgcaagag
      481 ccttaacaat atcgccagaa tacttgatac caccatcagc aataatcgtt ttgccatatt
      541 cacgagcaac agcagcagca tcgtaaatag cagtcacttg aggaacaccg acacctgcaa
      601 ccacacgagt ggtacagata gatccgggac caatacccac tttaacaaca tcaacacctg
      661 catcataaag agcacgcgca ccttcagcag tggcaatatt tccagcaatc aaagtacgat
      721 ttgggaaatg agcacgaatc tcagcaattt tacgaagaac accagccgaa tgaccatgag
      781 ctgtgtcaat aacaatcgca tctgcaccag cttcaaacaa ggcctcggca cgctcaaaag
      841 tatctgaggt aacacctacc gcagcagcga ccagaagacg accaaattca tctttagcag
      901 catgtgggaa ttcgattact ttttcaatat ctttgatagt gattaaacca gacagacgac
      961 cgctattatc aaccaaagga agtttttcaa tacggtgttc atgaagaata cgttctgcag
     1021 tttcaagatc tgtaccaaca gcagccgtaa cgaggtgctc actagtcata tgttcagaga
     1081 ttggtgcatt gtaatcagaa ataaaacgca tatcacgatt agtaatgata ccgaccaact
     1141 tacggtttgc taaagtttca acaattggca caccactgat acggtagcgc tgcattaatt
     1201 cttcagcttc tgaaaccttg tgttcaggtg ttaagaagaa tggatcaatg atgacaccat
     1261 tttcagaacg ttttaccttg cgaacttctt ctgcttgctc agtaatagac atgtttttat
     1321 gaataacacc taaaccaccc gcacgcgcaa ttgcaatagc cattttacta ccagtaaccg
     1381 tatccatagc tgctgtaata ataggaatat ttaatgtcaa attgtctgcg agttttgtct
     1441 ttaaatcaac ttcatttgga agaacatgac tctctgccgg aataagcaag acatcgtcaa
     1501 aagtgtagcc ttttttcaaa aatttagtgt cccaatttga catccagaca atcctctttt
     1561 cttttatttt tagggaatat taccctggtc tattttttat tgttactatc atatcacgct
     1621 aaaatcattt gtcaataaaa tattcctaca gctgaagtc
//
LOCUS       DATXIG010000113         1644 bp    DNA     linear   BCT 28-DEC-2023
DEFINITION  TPA_asm: Streptococcus pyogenes strain MGAS11113
            SAMN08555799-rid19682583.denovo.113, whole genome shotgun sequence.
ACCESSION   DATXIG010000113 DATXIG010000000
VERSION     DATXIG010000113.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN08555799
            Sequence Read Archive: SRR6740495
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pyogenes
  ORGANISM  Streptococcus pyogenes
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 1644)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1644)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-OCT-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-OCT-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001918058.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001918058.2
            Genome Coverage       :: 368x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/12/2023 11:23:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 1,806
            CDSs (total)                      :: 1,769
            Genes (coding)                    :: 1,722
            CDSs (with protein)               :: 1,722
            Genes (RNA)                       :: 37
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1, 1 (5S, 23S)
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 47
            CDSs (without protein)            :: 47
            Pseudo Genes (ambiguous residues) :: 0 of 47
            Pseudo Genes (frameshifted)       :: 30 of 47
            Pseudo Genes (incomplete)         :: 20 of 47
            Pseudo Genes (internal stop)      :: 9 of 47
            Pseudo Genes (multiple problems)  :: 11 of 47
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1644
                     /organism="Streptococcus pyogenes"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN08555799-rid19682583.denovo.113"
                     /strain="MGAS11113"
                     /serotype="Emm28"
                     /isolation_source="normally sterile site"
                     /host="Homo sapiens"
                     /db_xref="taxon:1314"
                     /country="Finland"
                     /collection_date="2002"
                     /collected_by="National Public Health Laboratory"
     gene            581..>1644
                     /locus_tag="VC122_001703"
     CDS             581..>1644
                     /locus_tag="VC122_001703"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012560532.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-glucose/GDP-mannose dehydrogenase family
                     protein"
                     /protein_id="HEP5641602.1"
                     /translation="MKITVVGIGYVGLSIGLLLAKEHDVTFFDIDNKKIDLINKRQSP
                     LKEAAINKLLCKAKNINATSSEELAYKDATFIILSLPTNLKFNKLDTSIIEISVSNIL
                     KINKKATIVIKSTVPIGFTEYLRNRFHYNDIIFSPEFLREGSTIHDQLYPSRTIVGNE
                     SRNSQLFLDILTDISVEKDSPSLLVGSSEAEAIKLFSNAYLAQKIAFFNELDTFAEMQ
                     NLDSKKIIEAMGYDQRIGNSHNNPSFGFGGYCLPKDIKQLEYHFKEIPAPIITSISES
                     NLLRKIHIAKMILNSSAKTIGIYRINSKKDSDNCRKSSTIDVAKLLKSSGKDVIIFEP
                     LINQKKFLGCPLINDFNEFI"
BASE COUNT          592 a          215 c          275 g          562 t
ORIGIN      
        1 ttttgatact ttgagtagta acgataatat ttaatttttt gatggattat tttaattgac
       61 tttagtgggc tagatgccag tttggatttt tgatatttgt gttgagttta aaagataaaa
      121 acacttaatg aaaatctgtt ttttcttaga aaggggacca gtatgattga gacgctaatg
      181 agtacgttgg aaatggttag aaaaaaaatt ctagtaggac tgcaatcgaa gatgactttg
      241 aggaatataa aaagagaaat ctaccaatct ttgtcagtga agatgatagt gtgatctatt
      301 atagactagg gttcattttt aatgaattct atctcaatat ttcaggctgg agacaagaac
      361 taaagactaa gcttactagt atttctttaa tttctttaca ttttattggt gttgttgacg
      421 aacaaaaaaa gggggaatat ctaggttctt tttctgatac atctgaattc ttaagtagac
      481 atccaaaaat ttatatcatc tcatttaggt ataaaaatca attttatatt ctttctaaca
      541 aaaaaattga tttataaaaa gttttagaat agaggttaac atgaaaatta cagttgtagg
      601 cattggatac gttggattat cgatagggct cctacttgca aaggaacacg acgtcacctt
      661 ttttgatatt gataataaaa aaattgattt aataaataaa aggcaatccc ctcttaaaga
      721 agcagctata aacaaacttt tatgtaaggc aaaaaatatt aatgcaactt cttctgaaga
      781 attagcatat aaggatgcga ctttcataat cttgtctttg ccaaccaacc taaaatttaa
      841 taagcttgat acttccatta tcgaaatttc tgtaagtaat attttaaaga taaacaaaaa
      901 ggctacaatt gtaataaagt cgacagttcc aattggtttt acagaatatt taaggaatcg
      961 atttcactac aacgatatca ttttttcacc tgagttcctt agggaaggat caactattca
     1021 tgatcaattg tatccttcga gaactatagt tggaaatgaa tctagaaatt ctcaattatt
     1081 cttagacata ctaacagata tatcggttga aaaagactcg ccatctttat tagttggctc
     1141 ttctgaagca gaagcgataa agttattttc gaatgcatac ttggcacaaa aaattgcttt
     1201 ttttaatgag ttggatacgt ttgctgaaat gcaaaatttg gactcaaaaa aaattattga
     1261 ggctatggga tatgaccaga gaataggaaa ttcgcacaat aatccttctt tcggttttgg
     1321 tgggtactgt cttcctaagg atattaagca attagagtat cattttaaag aaattccagc
     1381 accaattatt accagtataa gtgagtctaa tttattaaga aaaattcata tagcaaaaat
     1441 gattttgaac agctcagcta aaacaatagg aatttataga attaattcca aaaaagattc
     1501 ggataattgt aggaaatctt ctacaattga tgttgctaaa cttctaaaaa gcagtggtaa
     1561 ggatgttatc atctttgagc ccttaattaa ccaaaaaaag tttttggggt gccctttaat
     1621 taatgatttt aatgaattta ttaa
//
LOCUS       DATXIG010000114         1643 bp    DNA     linear   BCT 28-DEC-2023
DEFINITION  TPA_asm: Streptococcus pyogenes strain MGAS11113
            SAMN08555799-rid19682583.denovo.114, whole genome shotgun sequence.
ACCESSION   DATXIG010000114 DATXIG010000000
VERSION     DATXIG010000114.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN08555799
            Sequence Read Archive: SRR6740495
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pyogenes
  ORGANISM  Streptococcus pyogenes
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 1643)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1643)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-OCT-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-OCT-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001918058.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001918058.2
            Genome Coverage       :: 368x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/12/2023 11:23:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 1,806
            CDSs (total)                      :: 1,769
            Genes (coding)                    :: 1,722
            CDSs (with protein)               :: 1,722
            Genes (RNA)                       :: 37
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1, 1 (5S, 23S)
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 47
            CDSs (without protein)            :: 47
            Pseudo Genes (ambiguous residues) :: 0 of 47
            Pseudo Genes (frameshifted)       :: 30 of 47
            Pseudo Genes (incomplete)         :: 20 of 47
            Pseudo Genes (internal stop)      :: 9 of 47
            Pseudo Genes (multiple problems)  :: 11 of 47
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1643
                     /organism="Streptococcus pyogenes"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN08555799-rid19682583.denovo.114"
                     /strain="MGAS11113"
                     /serotype="Emm28"
                     /isolation_source="normally sterile site"
                     /host="Homo sapiens"
                     /db_xref="taxon:1314"
                     /country="Finland"
                     /collection_date="2002"
                     /collected_by="National Public Health Laboratory"
     gene            complement(<1..1079)
                     /locus_tag="VC122_001704"
     CDS             complement(<1..1079)
                     /locus_tag="VC122_001704"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002985164.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2142 domain-containing protein"
                     /protein_id="HEP5641603.1"
                     /translation="MQQSMTKVKYLWKENNNYIYSTFLTLLLTVILYANSLNLPLKFI
                     SLLFVVSTLLIVFLPKKLEVATIIFILVFGTISAIISPINDIPDEYVHYSRTVYISEG
                     DINLTNNNKKLRISKDVDKLIKQSGKTFITSNLKATKHSTREYSYPYIKGTNAYYSFS
                     YIPQALGILVGNALDLPILLTYYFGRLCNLISYAMLAFIAIKLSGSFKQVIAVVTLLP
                     MNIYLAASFNQDGFAIGLVLVTIGLFINLLSSKDKSNYNTKFFLYLVLCGLLVLSKFT
                     YFLLVCLPLFIPNEKFGKNTKLVILKKLGGLLLIFLFAAMWFRLYGQVKTPYVADFLK
                     EVNVSQQVKNMLESPIVYSSIIIRH"
     gene            complement(1060..>1643)
                     /locus_tag="VC122_001705"
     CDS             complement(1060..>1643)
                     /locus_tag="VC122_001705"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002990276.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="lipopolysaccharide biosynthesis protein"
                     /protein_id="HEP5641604.1"
                     /translation="AIELMTTLGEVALGSQTIFNILFMPAFVMNLLILFFRPHITQMA
                     IALIRGQIKEFNKIQVQLFAYLGVFSLIALVGSGLFGIPFLSILYGTNLTDYWVDFML
                     IMLGGSIGSFATVIDNILTAMRKQQLLLIPYTGGFLISLLITNLFVMKYHILGAALSF
                     LITMLVWLGLSIMIYLFIMNRFKKGRVNATIYD"
BASE COUNT          632 a          258 c          235 g          518 t
ORIGIN      
        1 atatgtctaa taataatact gctatagact ataggggatt ctagcatatt tttaacttgc
       61 tggctaacgt ttacttcttt tagaaaatcc gcgacatatg gggttttaac ctgtccataa
      121 agtctaaacc acattgcagc aaaaagaaaa atgagtaata gtcctcccaa ttttttcaag
      181 atgactaact tggtattttt tccgaatttt tcattaggaa taaataatgg aaggcatact
      241 aataaaaagt atgtgaattt tgaaagaact agtaacccac atagaactaa ataaaggaag
      301 aatttggtat tatagttaga tttatctttt gatgagagca agttaataaa caatccaatt
      361 gttactaaaa ctaaaccgat agcaaagccg tcttgattaa aagatgctgc taaataaatg
      421 ttcataggta gtaaagtaac aacagctatc acttgtttaa aagaaccaga caacttgata
      481 gctataaaag caagcatagc gtagctaata aggttgcaca atcttccgaa ataatatgtt
      541 aaaagtatag gtaaatccag cgcatttcct actaaaatac ccaaagcttg tggtatataa
      601 gaaaaactat aatatgcatt agttcctttg atgtaaggat agctatactc tcttgtgctg
      661 tgtttagttg ctttcaagtt tgatgttata aatgtttttc ctgactgttt aataagctta
      721 tcaacatctt tggatattct taactttttg ttattgttgg taagattaat atcaccttct
      781 gagatataaa ctgtccttga ataatgaaca tattcatcag gaatatcatt tataggtgat
      841 atgatagcag atatcgttcc aaatactaaa ataaaaatga tcgtagcaac ctctaacttt
      901 tttggtaaaa acactatcag taaagtgctc accacaaaaa gaagactaat aaattttaaa
      961 ggtagattta aactattggc atataaaata actgtcagaa gtaatgtcaa aaaagtcgaa
     1021 taaatatagt tattattttc tttccaaaga tatttaactt tagtcataga ttgttgcatt
     1081 aactcttccc ttcttaaatc tattcataat aaatagataa atcataatgg ataatcccaa
     1141 ccaaaccaac attgttatta aaaagctcaa agcagctcct aaaatatgat atttcataac
     1201 aaaaagattg gtaattaata acgaaatcaa aaatcctcct gtatatggaa taagaagaag
     1261 ttgctgtttt ctcatagcgg ttaagatatt atcgataact gtcgcaaaac taccaatcga
     1321 gcctcctagc ataatcaaca taaaatctac ccaataatcc gtaaggttag tgccatatag
     1381 tattgataaa aagggaatac caaacaaacc acttccaact aacgctatca gtgaaaaaac
     1441 tcctaaataa gcaaacaatt gaacttgtat cttattaaac tcttttattt gacctctgat
     1501 taaagcaata gccatctgtg taatatgagg tctaaaaaat aaaattagca aattcataac
     1561 aaaggctggc ataaataaaa tattaaaaat tgtttgagag cccaaagcaa cttcacctaa
     1621 agtcgtcatt agttcaatag cgt
//
LOCUS       DATXIG010000115         1601 bp    DNA     linear   BCT 28-DEC-2023
DEFINITION  TPA_asm: Streptococcus pyogenes strain MGAS11113
            SAMN08555799-rid19682583.denovo.115, whole genome shotgun sequence.
ACCESSION   DATXIG010000115 DATXIG010000000
VERSION     DATXIG010000115.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN08555799
            Sequence Read Archive: SRR6740495
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pyogenes
  ORGANISM  Streptococcus pyogenes
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 1601)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1601)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-OCT-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-OCT-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001918058.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001918058.2
            Genome Coverage       :: 368x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/12/2023 11:23:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 1,806
            CDSs (total)                      :: 1,769
            Genes (coding)                    :: 1,722
            CDSs (with protein)               :: 1,722
            Genes (RNA)                       :: 37
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1, 1 (5S, 23S)
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 47
            CDSs (without protein)            :: 47
            Pseudo Genes (ambiguous residues) :: 0 of 47
            Pseudo Genes (frameshifted)       :: 30 of 47
            Pseudo Genes (incomplete)         :: 20 of 47
            Pseudo Genes (internal stop)      :: 9 of 47
            Pseudo Genes (multiple problems)  :: 11 of 47
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1601
                     /organism="Streptococcus pyogenes"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN08555799-rid19682583.denovo.115"
                     /strain="MGAS11113"
                     /serotype="Emm28"
                     /isolation_source="normally sterile site"
                     /host="Homo sapiens"
                     /db_xref="taxon:1314"
                     /country="Finland"
                     /collection_date="2002"
                     /collected_by="National Public Health Laboratory"
     gene            complement(<1..922)
                     /locus_tag="VC122_001706"
     CDS             complement(<1..922)
                     /locus_tag="VC122_001706"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019213953.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MobA/MobL family protein"
                     /protein_id="HEP5641605.1"
                     /translation="MTDSFHFSVNIISRGKGKSAVASAAYISGEKIKNEWDGVIHDYT
                     RKEKILVKNIILPDHIPKEFNDRSTLWNKVEMAEKNSNAQLARQFIIGLPKELSLSEN
                     KNLVERFIKENLTSQGMIVDYAIHDESQDKNGNIHCHIMTIMRPINEKGEFLAKSKKE
                     YILDEKGEKILNKNGKPKTRKVELTIWNDKGNVEKWRENFSDLCNEYLAKNKIEKRVD
                     HRSFKRQNSDYLPTIHLGSAASAMERKGIETDKGNYNREIRKYNDLVKTIKEEIKTLK
                     GWIGNLLDNLSTAYEKFKDIERDKVIDNPKL"
     gene            complement(1237..1557)
                     /locus_tag="VC122_001707"
     CDS             complement(1237..1557)
                     /locus_tag="VC122_001707"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012512573.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3847 domain-containing protein"
                     /protein_id="HEP5641606.1"
                     /translation="MAIKRIKLSIEEQIEKKEESIKQLKNQKIQLKKKLNEQERKARN
                     KRLIEKGAVFESVFEESINLTIDEFYKLMKMLNDEEIRLNIMEILEERVDDDVEKSPK
                     DEIT"
BASE COUNT          376 a          275 c          193 g          757 t
ORIGIN      
        1 aaagtttagg gttgtctatt accttatctc tttctatatc cttaaatttt tcataagcag
       61 tagataggtt atctaataga tttcctatcc aaccctttaa tgtttttatc tcttctttta
      121 ttgtctttac aaggtcgtta tattttctta tttctctatt atagtttcct ttgtcagttt
      181 ctattccctt tctttccatt gcacttgctg ctgatcctaa atggattgtc ggtaaataat
      241 cagaattttg tcttttaaaa ctcctatggt ctactctttt ttctatcttg ttttttgcta
      301 gatattcatt gcaaaggtct gaaaaatttt ctctccattt ttctacattg cccttatcat
      361 tccagattgt tagttctact tttcttgttt taggttttcc atttttgttt aaaatctttt
      421 ctcctttttc atctaggata tattcttttt ttgactttgc taagaactct cctttttcgt
      481 ttattggtcg cattatggtc attatatgac aatggatatt tccatttttg tcttgactct
      541 catcatggat tgcataatct actatcattc cttgtgatgt tagattttct tttataaatc
      601 tttcgactag gtttttattt tcacttaaag ataattcttt tggtagtcct attatgaatt
      661 gtcttgctag ttgtgcatta gaattttttt ctgccatttc tactttattc cataatgtag
      721 acctatcatt aaattctttt ggtatatgat caggcaatat tatatttttt actaatattt
      781 tttcttttct tgtgtagtca tggattactc cgtcccattc attttttatt ttttctccac
      841 ttatatatgc tgcacttgct actgcacttt tcccttttcc tcttgatatt atgtttactg
      901 aaaaatgaaa actgtctgtc attttttatc acttcctttc tttttttgtt gttttaggtg
      961 gaggcttaat agatttttat atcctctttg tgaatgagcg ttttgaaatg atagaataga
     1021 aaaagccgac tactgaatta atttttgttg tttcagtatg tcggcttaat tgttttgttc
     1081 atttcaaatt ttaaaagtca atggcgagcc ttagcgagtt tatttaccct tgacttttaa
     1141 aaaacgaatg gttgttgctc cctgcaaggg ttttgctcta aaggcacgca agcacccttt
     1201 agggtgtata attgcgccct tgtaaccaag ggacaattat gttatttcat ctttaggtga
     1261 tttttctaca tcatcatcta ctctttcttc taaaatttcc attatgttta atcttatttc
     1321 ttcatcattt aacattttca ttaacttata aaattcatct attgttaaat taatactttc
     1381 ttcaaaaaca ctttcaaaca ctgcaccttt ttctataagt cttttgtttc ttgcttttct
     1441 ttcttgctca ttaagttttt tcttcagctg tattttttga ttttttaatt gcttaatgct
     1501 ttcttctttc ttttcgatct gttcttcaat acttaacttt attcttttaa ttgccatttg
     1561 tatcatttct taaaataaaa aaacgaatag atttttattt c
//
LOCUS       DATXIG010000116         1589 bp    DNA     linear   BCT 28-DEC-2023
DEFINITION  TPA_asm: Streptococcus pyogenes strain MGAS11113
            SAMN08555799-rid19682583.denovo.116, whole genome shotgun sequence.
ACCESSION   DATXIG010000116 DATXIG010000000
VERSION     DATXIG010000116.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN08555799
            Sequence Read Archive: SRR6740495
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pyogenes
  ORGANISM  Streptococcus pyogenes
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 1589)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1589)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-OCT-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-OCT-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001918058.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001918058.2
            Genome Coverage       :: 368x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/12/2023 11:23:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 1,806
            CDSs (total)                      :: 1,769
            Genes (coding)                    :: 1,722
            CDSs (with protein)               :: 1,722
            Genes (RNA)                       :: 37
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1, 1 (5S, 23S)
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 47
            CDSs (without protein)            :: 47
            Pseudo Genes (ambiguous residues) :: 0 of 47
            Pseudo Genes (frameshifted)       :: 30 of 47
            Pseudo Genes (incomplete)         :: 20 of 47
            Pseudo Genes (internal stop)      :: 9 of 47
            Pseudo Genes (multiple problems)  :: 11 of 47
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1589
                     /organism="Streptococcus pyogenes"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN08555799-rid19682583.denovo.116"
                     /strain="MGAS11113"
                     /serotype="Emm28"
                     /isolation_source="normally sterile site"
                     /host="Homo sapiens"
                     /db_xref="taxon:1314"
                     /country="Finland"
                     /collection_date="2002"
                     /collected_by="National Public Health Laboratory"
     gene            100..>1589
                     /locus_tag="VC122_001708"
     CDS             100..>1589
                     /locus_tag="VC122_001708"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011185060.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2079 domain-containing protein"
                     /protein_id="HEP5641607.1"
                     /translation="MIELNDEFIRKETIELANDGPRVHTTQYETKVPRLHKCYLLFFS
                     IIISSLTIAVPFLTDAANGLQSQNLYIGMMLTKGQLPYSDAFTTGGLFYFVIIALSYY
                     LGSTLWLVFVQVFCFYLSGLYLYKLINYMTCFQKVALTFSISYYLLSVSLGFGGLYPT
                     QLAMPFILISAWFLTKYFACLVKDEAFILFGFVGALAMLIDPSTLIFWSFACVTVFSY
                     NISQKHLARGFYQLLASIFGMILVFYTAGYFILNLQVLNPYLSQTMIYPFTFFKSGNL
                     SLLFGLAIQLFFALGLGLLTGMGNVIRRFKNNSDRVVKWLFVMVILESILVAIFSQDY
                     RPYHLLPLLPFGLILTAIPVGYQYGIGLGQSSHRRRHGKNGVGRVMMIYLKRHFYLPI
                     LIVGTILICSTYCFISSIPLNQERDHIASYLEQKLNKTQSIYVWDDTSKIYLDSKAKS
                     VSQFSSPDINTQKASHRKILEDELLENKAAYIVVNRYKNLPKIIQKV"
BASE COUNT          457 a          249 c          284 g          599 t
ORIGIN      
        1 taaaaaaata atggataata gctatgttag actagtgata tgaagaaata ttgcttttat
       61 tgaatcgtta gagaatatga tatttcaagg agaaaaatag tgatcgagtt aaacgatgaa
      121 ttcatccgaa aagagacgat agaactggcc aacgatggcc cacgagtaca caccactcaa
      181 tatgaaacta aagtacctcg gcttcataaa tgctatctgc ttttctttag cattattatc
      241 agttctttaa caattgcagt acctttttta acagatgcag ccaatggtct tcagtcccaa
      301 aacttatata taggaatgat gttaacgaag gggcaacttc cctatagtga tgcttttaca
      361 acaggagggc tcttttactt tgttatcatt gcattaagtt attatttggg atcgacactt
      421 tggctagtct ttgttcaggt gttttgtttt tacttatctg gtttatatct ttataaactc
      481 attaattata tgacatgctt tcaaaaagtg gctttaactt tttcaattag ctactatttg
      541 ttgtctgtta gtcttggttt tggaggattg tatcctactc aactggctat gccatttata
      601 ttaatatcgg cttggttttt aactaagtat tttgcctgtt tagtgaaaga tgaggcattt
      661 attctttttg gctttgtagg tgctcttgca atgctaattg acccgagtac ccttatcttt
      721 tggtcttttg cttgtgtgac agttttttct tataatataa gccaaaagca tcttgcaaga
      781 ggtttttatc aactgctagc ttcgattttt gggatgattt tagtttttta cacagcagga
      841 tatttcattt tgaacttaca agtgctaaat ccttatttat cacaaacgat gatttatcct
      901 tttacttttt ttaaatcagg aaacttatcg ttgctttttg gactggctat tcagttgttc
      961 ttcgctttgg ggcttggtct tttgacagga atggggaatg tcattaggcg atttaaaaac
     1021 aattctgata gggtcgtcaa gtggctattt gtcatggtca ttctagaatc tatacttgtg
     1081 gctatatttt cacaagacta tcgcccctat catcttttac ctcttttacc ttttggatta
     1141 attttgactg ctattcctgt tggctatcag tatggtatag gattaggtca gagtagtcat
     1201 cgcagacgtc atggtaaaaa tggtgttggt cgagtaatga tgatttatct taagagacac
     1261 ttttatttgc caattttaat tgtagggaca atactaatct gttctactta ttgtttcatt
     1321 agtagtattc ctcttaatca ggagcgtgat catattgcta gttatttaga acagaaacta
     1381 aataaaactc aatctattta tgtttgggat gatacttcta aaatttattt ggacagtaaa
     1441 gctaaatctg tttctcaatt tagttctcct gacatcaata cgcaaaaagc gagtcatcga
     1501 aaaatattag aagatgaact attagaaaat aaggctgctt atatcgttgt taatcgctat
     1561 aaaaacctgc ctaaaatcat tcaaaaagt
//
LOCUS       DATXIG010000117         1569 bp    DNA     linear   BCT 28-DEC-2023
DEFINITION  TPA_asm: Streptococcus pyogenes strain MGAS11113
            SAMN08555799-rid19682583.denovo.117, whole genome shotgun sequence.
ACCESSION   DATXIG010000117 DATXIG010000000
VERSION     DATXIG010000117.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN08555799
            Sequence Read Archive: SRR6740495
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pyogenes
  ORGANISM  Streptococcus pyogenes
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 1569)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1569)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-OCT-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-OCT-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001918058.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001918058.2
            Genome Coverage       :: 368x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/12/2023 11:23:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 1,806
            CDSs (total)                      :: 1,769
            Genes (coding)                    :: 1,722
            CDSs (with protein)               :: 1,722
            Genes (RNA)                       :: 37
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1, 1 (5S, 23S)
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 47
            CDSs (without protein)            :: 47
            Pseudo Genes (ambiguous residues) :: 0 of 47
            Pseudo Genes (frameshifted)       :: 30 of 47
            Pseudo Genes (incomplete)         :: 20 of 47
            Pseudo Genes (internal stop)      :: 9 of 47
            Pseudo Genes (multiple problems)  :: 11 of 47
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1569
                     /organism="Streptococcus pyogenes"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN08555799-rid19682583.denovo.117"
                     /strain="MGAS11113"
                     /serotype="Emm28"
                     /isolation_source="normally sterile site"
                     /host="Homo sapiens"
                     /db_xref="taxon:1314"
                     /country="Finland"
                     /collection_date="2002"
                     /collected_by="National Public Health Laboratory"
     gene            complement(156..770)
                     /locus_tag="VC122_001709"
     CDS             complement(156..770)
                     /locus_tag="VC122_001709"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011285558.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TVP38/TMEM64 family protein"
                     /protein_id="HEP5641608.1"
                     /translation="MTKRNSKAYILWQKIIKILGIIALIGTFFLAFWLYRLGILNDSN
                     ALKDLVQRYRLWGPFVFIVVQIIQIVFPVIPGGLTTVAGFLIFGPVTGFIYNYVGIII
                     GSIVLFLLVKTYGRKFILLFVNDKTFYKYERRLETPGYEKLFIFCMASPVSPADIMVM
                     ITGLTDMSLKRFVTILLITKPISIIGYSYLFIFGKDVISWFLQH"
     gene            862..1209
                     /gene="ssrA"
                     /locus_tag="VC122_001710"
     tmRNA           862..1209
                     /gene="ssrA"
                     /locus_tag="VC122_001710"
                     /product="transfer-messenger RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00023"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00023"
     gene            complement(1361..1549)
                     /locus_tag="VC122_001711"
     CDS             complement(1361..1549)
                     /locus_tag="VC122_001711"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002984426.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Paratox"
                     /protein_id="HEP5641609.1"
                     /translation="MLTYDEFKQAIDNGYITGDTVIIVRKNGQIFDYVLPGEKVRPWE
                     VVTEEVVEEVMVELDYIK"
BASE COUNT          534 a          299 c          239 g          497 t
ORIGIN      
        1 ttatcaaaaa taaactcaac ctacagtaag cgttaagact ttcttttaaa ctatgacaca
       61 ctatagttta aaagaaagtc tttttcagtg ttcatagtaa ataaaaaaac cgtcttccat
      121 caaatagaag cggtttatca aattaacacc aaaccttaat gctgtaagaa ccaagatata
      181 acatcttttc caaaaataaa taaataactg tagccgataa tagaaattgg tttagttatt
      241 aaaagaatag ttacaaagcg ctttaaagac atgtccgtca atcccgtaat cataaccata
      301 atatcagctg gagaaacagg cgatgccata caaaaaataa ataatttttc atatcctggt
      361 gtctccaaac gcctttcata cttgtaaaat gttttgtcat ttacaaagag taagataaat
      421 ttacgaccat aagtcttaac aagcaagaag aggactatac tgccaataat gatgcctaca
      481 taattataaa taaaacctgt tacagggcca aagattaaaa aacctgcaac tgtcgtaagg
      541 cctccaggaa tcacaggaaa aacaatctgt atgatctgca cgacaataaa aacaaacggc
      601 ccccataatc tatagcgctg cactaagtct ttaagagcat tactatcgtt taatatacct
      661 agtctataca gccaaaaagc gaggaaaaaa gtaccaatta gtgcaataat tcctaaaatt
      721 ttgataattt tttgccataa tatataggct tttgaattac gttttgtcat agactaatca
      781 taccataagc tgtaaaattt tgctatttaa aaaaagtttt gatagaatag ttagtgtaag
      841 aaattggtta attatctatt tggggttgtt acggattcga caggcattat gaggcatgtt
      901 ttgcgtccca tcggcagatg taaattgcca gttaaatata actgcaaaaa atacaaactc
      961 ttacgcttta gctgcctaaa aaccagctag cgtgacttct acaagattgc ttgtgtcctg
     1021 ttagaagtct caaaatagca agctacggtt acgaaattgt ctagtttcgt gacaagagat
     1081 tgatagactc gcaaactaat ggcttgagtt atgtgtcttt agtttgttaa atgaagacat
     1141 aacctatgga cgtagacaaa tatgttggca ggtgtttgga cgtgggttcg actcccacca
     1201 gctccatcaa tgcttaccgt aagtaatcat aacttactaa aaccttgtta catcaaggtt
     1261 ttttcttttt gtcttgttca tgagtttcca taactttcta tattattgac aactaaattg
     1321 acaactcttc aattattttt ctgtctactc aaagttttct tcatttgata tagtctaatt
     1381 ccaccatcac ttcttccact acctcctcgg tcacaacctc ccatggtctg actttctcac
     1441 caggcaacac ataatcaaaa atctgtccgt ttttgcgcac gattattact gtgtctcctg
     1501 tgatatatcc gttgtcaatt gcttgcttaa attcgtcgta tgttagcata aagcacctcc
     1561 ttatcttat
//
LOCUS       DATXIG010000118         1554 bp    DNA     linear   BCT 28-DEC-2023
DEFINITION  TPA_asm: Streptococcus pyogenes strain MGAS11113
            SAMN08555799-rid19682583.denovo.118, whole genome shotgun sequence.
ACCESSION   DATXIG010000118 DATXIG010000000
VERSION     DATXIG010000118.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN08555799
            Sequence Read Archive: SRR6740495
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pyogenes
  ORGANISM  Streptococcus pyogenes
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 1554)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1554)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-OCT-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-OCT-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001918058.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001918058.2
            Genome Coverage       :: 368x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/12/2023 11:23:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 1,806
            CDSs (total)                      :: 1,769
            Genes (coding)                    :: 1,722
            CDSs (with protein)               :: 1,722
            Genes (RNA)                       :: 37
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1, 1 (5S, 23S)
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 47
            CDSs (without protein)            :: 47
            Pseudo Genes (ambiguous residues) :: 0 of 47
            Pseudo Genes (frameshifted)       :: 30 of 47
            Pseudo Genes (incomplete)         :: 20 of 47
            Pseudo Genes (internal stop)      :: 9 of 47
            Pseudo Genes (multiple problems)  :: 11 of 47
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1554
                     /organism="Streptococcus pyogenes"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN08555799-rid19682583.denovo.118"
                     /strain="MGAS11113"
                     /serotype="Emm28"
                     /isolation_source="normally sterile site"
                     /host="Homo sapiens"
                     /db_xref="taxon:1314"
                     /country="Finland"
                     /collection_date="2002"
                     /collected_by="National Public Health Laboratory"
     gene            <1..562
                     /locus_tag="VC122_001712"
     CDS             <1..562
                     /locus_tag="VC122_001712"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002992712.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="sulfatase-like hydrolase/transferase"
                     /protein_id="HEP5641610.1"
                     /translation="SQFFSVITMQNHIPWSSDYPEEIVAEGKNFTEEENHNLTSYARL
                     LSFTDKETRAFLEKLTQINKPITVVFYGDHLPGLYPDSAFNKHIENKYLTDYFIWSNG
                     TNEKKNHPLINSSDFTAALFEHTDSKVSPYYALLTEVLNKASVDKSPDSPEVKAIQND
                     LKNIQYDVTIGKGYLLKHKTFFKISR"
     gene            741..1436
                     /locus_tag="VC122_001713"
     CDS             741..1436
                     /locus_tag="VC122_001713"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011017632.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HEP5641611.1"
                     /translation="MKKLIIIPAYNESSNIVNTIRTIESDAPDFDYIIIDDCSTDNTL
                     AICQKQGFNVISLPINLGIGGAVQTGYRYAQRCGYDVAVQVDGDGQHNPCYLEKMVEV
                     LVQSSVNMVIGSRFITKEGFQSSFARRIGIKYFTWLIALLTGKKITDATSGLRLIDRS
                     LIERFANHYPDDYPEPETVVDVLVSHFKVKEIPVVMNERQGGVSSISLTKSVYYMIKV
                     TLAILVVRLKGNR"
     gene            1438..>1554
                     /locus_tag="VC122_001714"
     CDS             1438..>1554
                     /locus_tag="VC122_001714"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002985167.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2304 domain-containing protein"
                     /protein_id="HEP5641612.1"
                     /translation="MTVTASLFISFIVLTFVFFLINLIKKDKLAIKYSLLWFI"
BASE COUNT          526 a          227 c          285 g          516 t
ORIGIN      
        1 aagccaattt ttctcagtta ttacaatgca aaatcatatt ccttggtcat ccgattatcc
       61 tgaagaaatt gttgctgaag gaaaaaattt cacggaagaa gaaaatcaca acctaacaag
      121 ttatgctcgg ttattatcgt ttactgataa ggaaacaaga gcatttttag aaaaattaac
      181 acaaattaac aagcctatca cagtggtgtt ttacggagat catttacccg gtttatatcc
      241 tgatagtgct tttaacaagc atattgaaaa taaatacctt actgattatt ttatttggag
      301 taatggtact aacgagaaaa aaaatcatcc gcttatcaac tcaagtgatt ttactgcagc
      361 tttatttgag catactgatt caaaagtatc accttactat gctttgttaa cagaggtact
      421 gaataaagct agtgtcgata aatcaccaga tagtcctgaa gttaaagcta ttcagaatga
      481 tttaaaaaat atccaatacg atgtgactat aggaaaaggt taccttttga aacacaaaac
      541 tttttttaag atatcacgtt aagaaaaatg ctaggctgtc cgagtataga cgagcatcaa
      601 ggtttggtaa tttgctcaaa tgattgtcaa cagagtgtct aggaccaggt ttaaaagatt
      661 ataattctta aggtcttatt cgtttataaa aaatgaatac tcttttaaaa tcttaattga
      721 aatgaatagg agaagttttc gtgaaaaagt taatcattat tcctgcttac aatgaaagca
      781 gtaatattgt caatactata cgtactattg aatcagatgc cccggatttt gactatatca
      841 ttattgatga ttgctcaacg gataatacgt tagcaatatg tcaaaaacag gggttcaatg
      901 ttatttcttt gcccattaac ctgggaattg gcggtgcggt gcaaactggc tatcgttatg
      961 cacaaagatg tggatatgac gttgcagttc aagtagatgg agatggtcag cacaatccat
     1021 gctatttgga aaaaatggtt gaggtattag ttcaatcttc agtaaatatg gtaattggat
     1081 cacgatttat cacaaaagaa gggtttcagt catcatttgc tcgacgtatc ggcataaagt
     1141 attttacttg gcttattgcg ctactaacag gaaaaaaaat aacagatgca acgtcaggtc
     1201 taaggttaat tgaccgttca ttgattgaac gttttgctaa tcattatcca gatgattatc
     1261 ctgaacctga aacagttgtt gatgtattgg ttagtcattt taaagtgaaa gaaattcctg
     1321 ttgtaatgaa tgagcgacaa ggcggtgtgt catctatctc gttgacgaaa tcggtctatt
     1381 atatgattaa agtgacttta gctattttag ttgttagatt gaaaggaaat cgttaaaatg
     1441 acggtaacag ccagtttatt tataagtttt attgtattaa cctttgtttt ctttttgatt
     1501 aacttaatca aaaaagataa actagcgatt aagtactctt tattgtggtt tatt
//
LOCUS       DATXIG010000119         1512 bp    DNA     linear   BCT 28-DEC-2023
DEFINITION  TPA_asm: Streptococcus pyogenes strain MGAS11113
            SAMN08555799-rid19682583.denovo.119, whole genome shotgun sequence.
ACCESSION   DATXIG010000119 DATXIG010000000
VERSION     DATXIG010000119.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN08555799
            Sequence Read Archive: SRR6740495
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pyogenes
  ORGANISM  Streptococcus pyogenes
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 1512)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1512)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-OCT-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-OCT-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001918058.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001918058.2
            Genome Coverage       :: 368x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/12/2023 11:23:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 1,806
            CDSs (total)                      :: 1,769
            Genes (coding)                    :: 1,722
            CDSs (with protein)               :: 1,722
            Genes (RNA)                       :: 37
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1, 1 (5S, 23S)
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 47
            CDSs (without protein)            :: 47
            Pseudo Genes (ambiguous residues) :: 0 of 47
            Pseudo Genes (frameshifted)       :: 30 of 47
            Pseudo Genes (incomplete)         :: 20 of 47
            Pseudo Genes (internal stop)      :: 9 of 47
            Pseudo Genes (multiple problems)  :: 11 of 47
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1512
                     /organism="Streptococcus pyogenes"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN08555799-rid19682583.denovo.119"
                     /strain="MGAS11113"
                     /serotype="Emm28"
                     /isolation_source="normally sterile site"
                     /host="Homo sapiens"
                     /db_xref="taxon:1314"
                     /country="Finland"
                     /collection_date="2002"
                     /collected_by="National Public Health Laboratory"
     gene            411..>1512
                     /locus_tag="VC122_001715"
     CDS             411..>1512
                     /locus_tag="VC122_001715"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002894089.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEP5641613.1"
                     /translation="MKLKLSVIGLFFCLIAAIGVISISDTQKPVPLPIDGAFSIQGKS
                     SLSSDEVYKKISNLSKTKNVNIYKPIVRNSGQLTYINFKNTNYEQLKPAPITGMYYTL
                     GKIDSSSLKVLTASGLKLVYAAYPWYIGGILQFDGTLRLLLMLSIYLTLLVVLFVVRT
                     RQIKEGVIRHSLGLPIYNLRRDYVISFTFELVMAGLLMIAYSYFWGSGLFTYSSKLFF
                     SLILTNLIIFQIVDIITFILFWLTIRVEKPIEIIKNKAKNSFLFIVWLVIISIIIIVS
                     GIFLQETKASQSRINRQISQLSSWNKVRNWKRLELLGIENGSINNGEINEPESQYIQI
                     ATALKKLDFIYIKPSSVYIPDYMKNTNFAEDFF"
BASE COUNT          520 a          204 c          259 g          529 t
ORIGIN      
        1 tagtataaaa aaagatttaa tagcaaagtg ataactttgc ttgctgtaag ctctttaagt
       61 ttgggattta cggccgtaac tagtgttgtc agtgcggcaa cagcacatcc agctccatct
      121 ccaattattg atgaatggaa ttatggagta agtgacaact atggttattc taattattat
      181 gttaaatctc caaacaatat tggttcaaaa tcttcgataa ctaattattg gggatccatt
      241 aaggcatctg attctcaaaa aatacgggtg ggcactgtca agttcaaaaa aagcttggaa
      301 tgatattcgg ttgaatgcac attggaatta ctttaatttt tagaaatggt atatgcaagc
      361 taaagaccca ataattatta tagggtcttt tataatatag gaagttgttt atgaaattaa
      421 aattatctgt cattggatta ttcttttgct taattgctgc gataggtgtt atcagtatta
      481 gtgatactca aaaaccggta cctttaccaa ttgatggtgc cttttcaatc caaggaaaaa
      541 gtagcctttc tagtgacgaa gtttacaaaa agattagtaa tctatcaaaa acgaaaaacg
      601 ttaatattta caagcctata gttcgtaact caggtcagtt gacttatatc aattttaaaa
      661 atacaaatta tgaacaatta aaacctgcac cgataacagg catgtattat accttaggta
      721 aaatagattc aagtagctta aaggtactta cagcttcagg tttgaaactt gtttatgcag
      781 cctatccttg gtatataggg gggattttac aatttgatgg gactttaagg ttgttattga
      841 tgctatcaat ttacttaaca ttattagttg ttttatttgt tgttagaacg aggcagatta
      901 aagaaggagt gataagacat tctttaggat tgcctatata taacttaaga agagattatg
      961 tcatttcttt tacttttgaa ttggttatgg ctggtttatt aatgattgcc tacagctatt
     1021 tttggggaag tggcctcttt acttacagtt ctaagttatt tttctctcta attttaacta
     1081 atcttataat attccagatt gttgacatta tcacttttat tttattttgg ctaactatta
     1141 gggttgagaa acctattgaa attataaaaa ataaagcaaa aaatagtttt ctttttattg
     1201 tatggcttgt aattatttct attataataa tcgtctcagg aatcttttta caagaaacaa
     1261 aggctagtca atcaaggata aatagacaga taagtcaact atcttcatgg aacaaagtaa
     1321 gaaactggaa aagactagag ttgttgggga ttgaaaatgg ctctattaat aatggggaaa
     1381 taaatgagcc agaatcacaa tatatacaga tagcgacagc cttaaaaaaa ttagatttta
     1441 tatatatcaa accctcttca gtatatattc cagattacat gaaaaatact aactttgcag
     1501 aagatttttt ca
//
LOCUS       DATXIG010000120         1489 bp    DNA     linear   BCT 28-DEC-2023
DEFINITION  TPA_asm: Streptococcus pyogenes strain MGAS11113
            SAMN08555799-rid19682583.denovo.120, whole genome shotgun sequence.
ACCESSION   DATXIG010000120 DATXIG010000000
VERSION     DATXIG010000120.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN08555799
            Sequence Read Archive: SRR6740495
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pyogenes
  ORGANISM  Streptococcus pyogenes
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 1489)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1489)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-OCT-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-OCT-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001918058.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001918058.2
            Genome Coverage       :: 368x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/12/2023 11:23:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 1,806
            CDSs (total)                      :: 1,769
            Genes (coding)                    :: 1,722
            CDSs (with protein)               :: 1,722
            Genes (RNA)                       :: 37
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1, 1 (5S, 23S)
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 47
            CDSs (without protein)            :: 47
            Pseudo Genes (ambiguous residues) :: 0 of 47
            Pseudo Genes (frameshifted)       :: 30 of 47
            Pseudo Genes (incomplete)         :: 20 of 47
            Pseudo Genes (internal stop)      :: 9 of 47
            Pseudo Genes (multiple problems)  :: 11 of 47
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1489
                     /organism="Streptococcus pyogenes"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN08555799-rid19682583.denovo.120"
                     /strain="MGAS11113"
                     /serotype="Emm28"
                     /isolation_source="normally sterile site"
                     /host="Homo sapiens"
                     /db_xref="taxon:1314"
                     /country="Finland"
                     /collection_date="2002"
                     /collected_by="National Public Health Laboratory"
     gene            181..1251
                     /gene="xerS"
                     /locus_tag="VC122_001716"
     CDS             181..1251
                     /gene="xerS"
                     /locus_tag="VC122_001716"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012658412.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine recombinase XerS"
                     /protein_id="HEP5641614.1"
                     /translation="MRRELLLEKIETYKAVMPWYVLDYYQSKLAVPYSFTTLYEYLKE
                     YKRFFDWLMDADLTQAPKIADIDLSTLEHLTKKDLEAFVLYLRERPSLNTYSTKEGLS
                     QTTINRTLSALSSLYKYLTEEVENDQGEPYFYRNVMKKVSTKKKKETLASRAENIKQK
                     LFLGDETLAFLDYVDKEYEQKLSNRAKSSFRKNKERDLAIIALLLASGVRLSEAVNLD
                     LKDVNLNMMIIEVIRKGGKRDSVNVAGFAKGYLESYLAVRQRRYKAEKQDLAFFLTEY
                     RGVPNRMDASSIEKMVGKYSEDFKIRVTPHKLRHTLATRLYDATKSQVLVSHQLGHSS
                     TQVTDLYTHIVNDEQKNALDNL"
BASE COUNT          479 a          241 c          286 g          483 t
ORIGIN      
        1 agtatctgta aaaaaagaga tttgtctcgt gacatctttc cgaaaaactg taattttctt
       61 gaaaaatatt tataggtgtg atatactgct cttgtagaga atgaatggca taaaatacat
      121 gagacaattt gcgaaaattc atttttgaaa aaattgttat tgttaaggaa ttatgatgat
      181 atgagacgcg aattattact agaaaaaatt gaaacctaca aggctgtcat gccttggtat
      241 gttttagatt attaccaatc caaattagct gttccataca gctttaccac cttatatgag
      301 tatttaaaag aatacaaacg cttttttgat tggctgatgg atgctgattt aacgcaggcg
      361 ccaaagattg ctgatattga cttgagcacg cttgagcacc ttaccaagaa ggatttagag
      421 gcctttgtgc tttatttgcg ggaacgacct tctctcaaca cctattccac caaagagggg
      481 ctttctcaaa ccactattaa tcggaccttg tctgccttat caagccttta caagtacttg
      541 actgaagagg ttgaaaatga ccaaggggag ccttattttt atcggaatgt tatgaaaaaa
      601 gtgtcaacaa aaaagaaaaa agaaaccctg gcctccagag cggaaaatat taagcagaaa
      661 ctgtttttag gtgatgaaac tctagctttc ttggattatg tggataagga atatgagcaa
      721 aaattgtcta atcgtgccaa atcttctttc cgtaaaaata aggagaggga tttggctatt
      781 attgctctac tacttgcttc tggtgttcgt ttgtctgaag cagtgaatct tgatttaaaa
      841 gatgtcaatc taaatatgat gattatcgaa gttattcgca aaggcggaaa acgtgattca
      901 gtcaatgtag caggttttgc gaaaggttat ttggagtctt atttagctgt tcgtcagaga
      961 cgttacaagg ctgaaaaaca ggatcttgct ttctttttaa cagaataccg tggcgttcca
     1021 aatcgaatgg atgcttctag tatagaaaag atggttggca agtactctga ggattttaaa
     1081 attcgtgtga ctcctcacaa attgcgtcat acgctagcga caagactata tgatgctact
     1141 aaatctcagg tgttagttag tcatcaatta ggccattcct ctactcaagt cacggatctt
     1201 tatacccata ttgtcaatga tgagcaaaaa aatgctttag ataaccttta atgttacata
     1261 attgttttta tgtaacatta aagataaaaa tagcttttaa attaacttta atagatgact
     1321 actattaacc gtgacagttt ggtgtcaggc cgttttccaa actactcaag ccacttagaa
     1381 aaaggaatat tactgctaac tttgtccctt ctttgaatga cttttttgtg atgtttatag
     1441 attaaatcta gtagggttct catcaaaaag atgagataag cctgttttt
//
LOCUS       DATXIG010000121         1455 bp    DNA     linear   BCT 28-DEC-2023
DEFINITION  TPA_asm: Streptococcus pyogenes strain MGAS11113
            SAMN08555799-rid19682583.denovo.121, whole genome shotgun sequence.
ACCESSION   DATXIG010000121 DATXIG010000000
VERSION     DATXIG010000121.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN08555799
            Sequence Read Archive: SRR6740495
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pyogenes
  ORGANISM  Streptococcus pyogenes
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 1455)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1455)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-OCT-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-OCT-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001918058.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001918058.2
            Genome Coverage       :: 368x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/12/2023 11:23:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 1,806
            CDSs (total)                      :: 1,769
            Genes (coding)                    :: 1,722
            CDSs (with protein)               :: 1,722
            Genes (RNA)                       :: 37
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1, 1 (5S, 23S)
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 47
            CDSs (without protein)            :: 47
            Pseudo Genes (ambiguous residues) :: 0 of 47
            Pseudo Genes (frameshifted)       :: 30 of 47
            Pseudo Genes (incomplete)         :: 20 of 47
            Pseudo Genes (internal stop)      :: 9 of 47
            Pseudo Genes (multiple problems)  :: 11 of 47
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1455
                     /organism="Streptococcus pyogenes"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN08555799-rid19682583.denovo.121"
                     /strain="MGAS11113"
                     /serotype="Emm28"
                     /isolation_source="normally sterile site"
                     /host="Homo sapiens"
                     /db_xref="taxon:1314"
                     /country="Finland"
                     /collection_date="2002"
                     /collected_by="National Public Health Laboratory"
     gene            92..1351
                     /locus_tag="VC122_001717"
     CDS             92..1351
                     /locus_tag="VC122_001717"
                     /EC_number="2.5.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001226236.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylglucosamine
                     1-carboxyvinyltransferase"
                     /protein_id="HEP5641615.1"
                     /translation="MRKIIINGGKALSGEVAVSGAKNSVVALIPAIILADDIVILDGV
                     PAISDVDSLIEIMELMGATVNYHGDTLEIDPRGVQDIPMPYGKINSLRASYYFYGSLL
                     GRFGQAVVGLPGGCDLGPRPIDLHLKAFEAMGVEVSYEGENMNLSTNGQKIHGAHIYM
                     DTVSVGATINTMVAATKAQGKTVIENAAREPEIIDVATLLNNMGAHIRGAGTDIITIQ
                     GVQKLHGTRHQVIPDRIEAGTYIALAAAIGKGVKITNVLYEHLESFIAKLEEMGVRMT
                     VEEDAIFVEKQESLKAITIKTSPYPGFATDLQQPLTPLLLKADGRGTIIDTIYEKRIN
                     HVPELMRMGADISVIGGQIVYQGPSRLTGAQVKATDLRAGAALVTAGLIAEGKTEITN
                     IEFILRGYASIIAKLTALGADIQLIED"
BASE COUNT          434 a          236 c          338 g          447 t
ORIGIN      
        1 gattaaaaat taagacgtca ataattgtca ggcttcatga tttatgataa aatgaaaagg
       61 ttgaattcta gtataagaaa gttttgaatg tatgcgtaaa attattatta atggcggaaa
      121 agccttatct ggagaagtgg cagtttcagg ggcaaaaaac agtgttgttg cgcttattcc
      181 agcaatcatc ttagccgatg atattgtgat tcttgatggt gtacctgcta ttagtgatgt
      241 agatagtctg attgaaatca tggagcttat gggagcaaca gtaaactatc atggtgatac
      301 tttagaaatt gaccctagag gggtgcaaga catcccgatg ccttacggaa agattaatag
      361 tcttcgagct tcctattatt tctacggtag tcttttaggt cgttttggac aagcagttgt
      421 tggcttacct ggtggttgcg atttaggacc aagaccaatt gacttgcatt tgaaggcctt
      481 cgaggctatg ggagttgaag tgtcttatga gggtgaaaat atgaatttat cgacaaatgg
      541 tcaaaaaatt catggcgctc atatctatat ggacaccgtt agtgtcggtg ctacaattaa
      601 tactatggtt gcggcaacaa aagctcaagg taaaacggtt attgaaaacg ctgcacgtga
      661 gccagagatt attgatgtgg ctactctttt gaataacatg ggtgcgcata ttcgtggagc
      721 aggtacagat attattacga tacaaggtgt tcagaaattg catggtacta gacaccaagt
      781 tattcctgat cgtattgaag cagggactta tatcgctttg gcggcagcta ttggaaaagg
      841 tgttaagatc actaatgttc tttatgagca tttagagagt ttcattgcta agttagaaga
      901 aatgggtgtc cgtatgacgg tagaagagga tgctatcttt gttgaaaagc aagaatccct
      961 caaggctatt acaattaaaa cctctcctta ccctggtttt gccactgatt tgcaacagcc
     1021 attaacgccc ttgttgttaa aagcagatgg tcgtggaacg attattgaca ctatatatga
     1081 aaagcgcatt aaccatgtgc cagagttgat gcgcatggga gcagatatct cagttattgg
     1141 tggtcagatt gtttatcaag ggccgtctcg cctgacagga gctcaggtca aagccactga
     1201 tttacgtgcc ggagcggctc tagtgactgc tggtctaata gctgaaggca agacagaaat
     1261 tactaatatt gaatttatct tacgtggcta tgctagtatc attgcaaaat taacagcact
     1321 aggggcagat attcaattaa tcgaagacta aagagaagct aacgcttctc ttttttatat
     1381 tacatgtgct taggctaata atacttaggt gcatactttg ttgcatttta tggcttttta
     1441 actgtcataa aaaat
//
LOCUS       DATXIG010000122         1385 bp    DNA     linear   BCT 28-DEC-2023
DEFINITION  TPA_asm: Streptococcus pyogenes strain MGAS11113
            SAMN08555799-rid19682583.denovo.122, whole genome shotgun sequence.
ACCESSION   DATXIG010000122 DATXIG010000000
VERSION     DATXIG010000122.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN08555799
            Sequence Read Archive: SRR6740495
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pyogenes
  ORGANISM  Streptococcus pyogenes
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 1385)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1385)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-OCT-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-OCT-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001918058.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001918058.2
            Genome Coverage       :: 368x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/12/2023 11:23:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 1,806
            CDSs (total)                      :: 1,769
            Genes (coding)                    :: 1,722
            CDSs (with protein)               :: 1,722
            Genes (RNA)                       :: 37
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1, 1 (5S, 23S)
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 47
            CDSs (without protein)            :: 47
            Pseudo Genes (ambiguous residues) :: 0 of 47
            Pseudo Genes (frameshifted)       :: 30 of 47
            Pseudo Genes (incomplete)         :: 20 of 47
            Pseudo Genes (internal stop)      :: 9 of 47
            Pseudo Genes (multiple problems)  :: 11 of 47
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1385
                     /organism="Streptococcus pyogenes"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN08555799-rid19682583.denovo.122"
                     /strain="MGAS11113"
                     /serotype="Emm28"
                     /isolation_source="normally sterile site"
                     /host="Homo sapiens"
                     /db_xref="taxon:1314"
                     /country="Finland"
                     /collection_date="2002"
                     /collected_by="National Public Health Laboratory"
     gene            29..802
                     /locus_tag="VC122_001718"
     CDS             29..802
                     /locus_tag="VC122_001718"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004270277.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DeoR/GlpR transcriptional regulator"
                     /protein_id="HEP5641616.1"
                     /translation="MKKKERHEKILDILKVDGFIKVKDIIDEMNISDMTARRDLDTLA
                     DKGLLIRTHGGAQYLDYSSAKDEGHEKTHTEKKVLQTTEKKQIAQRAKNIIKDGETVF
                     IGPGTTLEHLAVELKNRNIRVITNSLPVFLILHPNTTIDLILIGGECREITGAFVGAI
                     AINNLESLTFSKAFVSANAVYNNSIATYSDLEGEVQRVALNNAVEKILLVDSTKFESY
                     DFYNFYQLDQVDLIVTDKNVTTDIIEKYKKFSKIIIADK"
BASE COUNT          481 a          216 c          245 g          443 t
ORIGIN      
        1 taccctaaga cactgatagg agataagaat gaaaaagaaa gagcgtcatg aaaaaattct
       61 cgatatctta aaagttgacg gttttataaa agtaaaagat attattgatg aaatgaacat
      121 ctcagatatg accgctagac gagatctcga tacattggca gataaaggat tacttatcag
      181 gacccatggt ggtgctcaat atttggatta ttcttcagca aaggacgaag gtcacgagaa
      241 aacacacact gaaaagaagg tcttacagac taccgaaaaa aaacaaattg cacaaagagc
      301 taaaaacatt attaaagacg gcgaaactgt ctttataggt ccagggacaa cattagagca
      361 tctagctgtc gaattaaaaa atagaaatat cagggtcatt acaaatagcc ttcctgtttt
      421 tttaattttg cacccgaaca ctactattga tttgatttta attggtggtg aatgtcgtga
      481 aattactgga gcttttgttg gtgctattgc cattaataat ctagaatcat taactttttc
      541 aaaggctttt gttagcgcaa atgcagttta caataattct attgcgacgt acagtgatct
      601 cgaaggagaa gttcaaaggg ttgcattgaa taatgctgtt gagaaaatcc ttctagttga
      661 tagtaccaag tttgaaagtt atgacttcta taatttttac caactagatc aagtggactt
      721 aattgtgact gataaaaatg tgacaactga tatcatagaa aagtacaaga aattctcaaa
      781 aatcataatc gctgacaagt aaaggaccta ctcatattaa aaagcacttc agggtttaga
      841 tatatcccct gtcaagtaaa cagggagcat atctgaaagt agtgtttgtg ttataaagaa
      901 gtttcttgct aacttatttt ctggtttttt tcagacttat cagtcttgta ctgttccttt
      961 ttctgatgaa taacgtggaa ataggcagac agctatatat ttcacctcta gaatggttgt
     1021 cattactgtc atcaagacat gcttgttctt tatcgtatgg agtgaagctt tcttttagac
     1081 atcaacatac ctcttaagta gattttggtt cgttaccatg tctacttaag aggtgtcttc
     1141 aaagcgcttt aaaacgttga tataacagta tattaacgtt ttgagaattg tgaagcttta
     1201 cgagcttttt taagacctgg tttttggttc actccattat ttaaagtcaa atccctttaa
     1261 aatcaatgtt tctaaaatga aaaatcacta aaaatcttat ttatagacaa atatttaaac
     1321 ctaaacggta tcaaaaataa gtttcggagc ttatcaaata atgaaaatct gtctcaatta
     1381 ccgag
//
LOCUS       DATXIG010000123         1378 bp    DNA     linear   BCT 28-DEC-2023
DEFINITION  TPA_asm: Streptococcus pyogenes strain MGAS11113
            SAMN08555799-rid19682583.denovo.123, whole genome shotgun sequence.
ACCESSION   DATXIG010000123 DATXIG010000000
VERSION     DATXIG010000123.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN08555799
            Sequence Read Archive: SRR6740495
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pyogenes
  ORGANISM  Streptococcus pyogenes
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 1378)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1378)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-OCT-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-OCT-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001918058.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001918058.2
            Genome Coverage       :: 368x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/12/2023 11:23:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 1,806
            CDSs (total)                      :: 1,769
            Genes (coding)                    :: 1,722
            CDSs (with protein)               :: 1,722
            Genes (RNA)                       :: 37
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1, 1 (5S, 23S)
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 47
            CDSs (without protein)            :: 47
            Pseudo Genes (ambiguous residues) :: 0 of 47
            Pseudo Genes (frameshifted)       :: 30 of 47
            Pseudo Genes (incomplete)         :: 20 of 47
            Pseudo Genes (internal stop)      :: 9 of 47
            Pseudo Genes (multiple problems)  :: 11 of 47
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1378
                     /organism="Streptococcus pyogenes"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN08555799-rid19682583.denovo.123"
                     /strain="MGAS11113"
                     /serotype="Emm28"
                     /isolation_source="normally sterile site"
                     /host="Homo sapiens"
                     /db_xref="taxon:1314"
                     /country="Finland"
                     /collection_date="2002"
                     /collected_by="National Public Health Laboratory"
     gene            210..1331
                     /gene="mnmA"
                     /locus_tag="VC122_001719"
     CDS             210..1331
                     /gene="mnmA"
                     /locus_tag="VC122_001719"
                     /EC_number="2.8.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017769308.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA 2-thiouridine(34) synthase MnmA"
                     /protein_id="HEP5641617.1"
                     /translation="MTDNSKIRVVVGMSGGVDSSVTALLLKEQGYDVIGVFMKNWDDT
                     DEFGVCTATEDYKDVAAVADQIGIPYYSVNFEKEYWDRVFEYFLAEYRAGRTPNPDVM
                     CNKEIKFKAFLDYAMTLGADYVATGHYAQVKRDENGTVHMLRGADNGKDQTYFLSQLS
                     QEQLQKTLFPLGHLQKSEVREIAERAGLATAKKKDSTGICFIGEKNFKQFLSQYLPAQ
                     KGRMMTIDGRDMGEHAGLMYYTIGQRGGLGIGGQHGGDNQPWFVVGKDLSQNILYVGQ
                     GFYHEALMSNSLDASVIHFTREMPEEFTFECTAKFRYRQPDSHVAVHVRGDKAEVVFA
                     EPQRAITPGQAVVFYDGKECLGGGMIDMAYKNGQPCQYI"
BASE COUNT          423 a          232 c          306 g          417 t
ORIGIN      
        1 aaaaaattat tgagcaataa aatgattggc attccacgca gtaattgtta taataaggtt
       61 tgcgctgatg gcgcttcaaa atcgaccttc aaatcgactt ttgaaaacga cgaccttcaa
      121 tcgtacgtta actgataaga aaaagaatca taatattcat aatatgatga ttatcttttt
      181 ctatcttatt attgatggga gaatttttta tgacagataa ctcaaagata cgtgttgtcg
      241 ttggtatgag tggtggcgtt gattcatctg taacagctct acttttaaag gagcaaggtt
      301 atgatgttat tggtgtcttc atgaaaaact gggacgatac agatgaattt ggtgtttgca
      361 cagctactga agattacaaa gatgtagcag ccgttgctga tcaaatcggt attccttact
      421 actcagtcaa ttttgaaaaa gaatattggg acagagtatt tgaatatttc ttggccgaat
      481 accgagcagg tcgtactcca aatcccgatg tcatgtgtaa taaggaaata aaatttaaag
      541 ctttccttga ctatgccatg actttaggtg cagattatgt agcaacggga cactatgctc
      601 aagtcaagcg tgatgaaaat ggtacggttc acatgttacg tggagctgat aatggcaaag
      661 accaaaccta ctttttaagt caactatcac aggaacaact acaaaagact ttattccccc
      721 ttggtcattt gcaaaagtca gaagttagag aaatcgctga gcgagcagga cttgcaacgg
      781 ctaaaaagaa agattcaaca ggtatttgtt ttattggtga aaagaatttc aaacagtttt
      841 tgagccagta cttaccggca caaaaaggtc gcatgatgac tattgatggt cgcgatatgg
      901 gagaacatgc cggtcttatg tattatacta ttggtcagcg tggtggtctg ggcattggtg
      961 gtcagcatgg tggggataat caaccttggt ttgttgtcgg taaagatttg tcgcaaaata
     1021 ttttatacgt tggtcaaggt ttctaccatg aagccctaat gtcaaatagt cttgacgcct
     1081 cagtcatcca ttttacacgt gagatgccag aagagttcac atttgaatgt actgctaaat
     1141 ttcgctatcg tcaacccgat tcacacgtgg cggtgcatgt tcgaggagat aaggcagaag
     1201 ttgtttttgc agagccacag cgcgcgatta ctcctggtca agctgttgtt ttctatgacg
     1261 gaaaagaatg ccttggtggt ggtatgattg acatggctta taaaaatggg cagccttgtc
     1321 agtacattta atgaatcttt tattaagata aaaagaaagg gacattcaac aattcctt
//
LOCUS       DATXIG010000124         1378 bp    DNA     linear   BCT 28-DEC-2023
DEFINITION  TPA_asm: Streptococcus pyogenes strain MGAS11113
            SAMN08555799-rid19682583.denovo.124, whole genome shotgun sequence.
ACCESSION   DATXIG010000124 DATXIG010000000
VERSION     DATXIG010000124.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN08555799
            Sequence Read Archive: SRR6740495
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pyogenes
  ORGANISM  Streptococcus pyogenes
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 1378)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1378)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-OCT-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-OCT-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001918058.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001918058.2
            Genome Coverage       :: 368x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/12/2023 11:23:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 1,806
            CDSs (total)                      :: 1,769
            Genes (coding)                    :: 1,722
            CDSs (with protein)               :: 1,722
            Genes (RNA)                       :: 37
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1, 1 (5S, 23S)
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 47
            CDSs (without protein)            :: 47
            Pseudo Genes (ambiguous residues) :: 0 of 47
            Pseudo Genes (frameshifted)       :: 30 of 47
            Pseudo Genes (incomplete)         :: 20 of 47
            Pseudo Genes (internal stop)      :: 9 of 47
            Pseudo Genes (multiple problems)  :: 11 of 47
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1378
                     /organism="Streptococcus pyogenes"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN08555799-rid19682583.denovo.124"
                     /strain="MGAS11113"
                     /serotype="Emm28"
                     /isolation_source="normally sterile site"
                     /host="Homo sapiens"
                     /db_xref="taxon:1314"
                     /country="Finland"
                     /collection_date="2002"
                     /collected_by="National Public Health Laboratory"
     gene            <1..>1378
                     /locus_tag="VC122_001720"
     CDS             <1..>1378
                     /locus_tag="VC122_001720"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002990440.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1542 domain-containing protein"
                     /protein_id="HEP5641618.1"
                     /translation="KSGDPIEARRGLHNKSIDEVAQATKDAITADTTLTEAEKETQRG
                     NVDKEATKAKEELAKAKDADALDKAYGDGVTSIKNQHKSGKGLDVRKDEHKKALEAVA
                     KRVTAEIEADPTLTPEVREQQKAEVQKELELATDKIAEAKDADEADKAYGDGVTAIEN
                     AHVIGKGIEARKDLAKKDLAEAAAKTKALIIEDKTLTDDQRKEQLLGVDTEYAKGIEN
                     IDAAKDAAGVDKAYSDGVRDILAQYKEGQNLNDRRNAAKEFLLKEADKVTKLINDDPT
                     LTHDQKVDQINKVEQAKLDAIKSVDDAQTADAINDALGKGIENINNQYQHGDGVDVRK
                     ATAKDDLEKEAAKVKALIAKDPTLTQADKDKQTAAVDAAKNTAIAAVDKATTADGVNQ
                     ELGKGITAINKAYRPGEAVKARKEAAKADLEKEAAKVKALIAKDPTLTQAAKDKQTAA
                     VDAAKNTAI"
BASE COUNT          504 a          266 c          315 g          293 t
ORIGIN      
        1 aagtcaggtg accctatcga agctcgtcgt ggattacaca acaagtcaat cgacgaagtg
       61 gcgcaagcaa ctaaggacgc tatcacagca gatacgactt tgactgaagc tgaaaaagaa
      121 acacaacgtg gcaatgttga taaagaagca actaaagcta aagaagaact tgctaaggct
      181 aaagatgctg atgctttaga caaagcgtat ggtgacggtg taaccagcat caagaaccaa
      241 cacaagtcag gtaaaggtct tgacgttcgt aaagatgagc acaagaaagc tcttgaagct
      301 gttgctaaac gtgtcactgc tgaaattgag gctgatccaa ccttaacacc agaagtgaga
      361 gaacaacaaa aagcagaggt tcaaaaagag cttgaacttg cgactgataa gattgctgaa
      421 gctaaagatg cagatgaagc agacaaagct tacggtgacg gtgtcacagc gatcgaaaat
      481 gcccacgtta ttggtaaagg tatcgaagct cgtaaagacc ttgctaagaa agaccttgct
      541 gaagctgctg ctaagacaaa agctctcatt attgaagaca aaacgcttac tgatgatcaa
      601 cgtaaagaac agttattagg tgttgataca gagtatgcta aaggtatcga gaatattgat
      661 gcagctaagg atgctgcagg tgttgataaa gcatatagtg acggtgttcg tgacatcctg
      721 gcacagtaca aagaaggtca aaaccttaat gatcgtcgta atgctgccaa agaatttctt
      781 cttaaagaag cagacaaagt gacgaaacta atcaatgatg atccaacctt gactcatgac
      841 caaaaagttg atcaaatcaa caaagttgaa caagctaagt tagacgcaat caagtctgtt
      901 gatgatgctc aaacagctga tgctatcaat gatgctcttg gtaagggtat tgaaaacatc
      961 aacaaccaat accaacatgg cgatggcgtt gatgttcgta aagcgactgc caaagacgat
     1021 cttgaaaaag aagctgctaa agtgaaagct cttattgcta aggatccaac cttgactcaa
     1081 gctgataaag acaagcaaac agcagcggtt gacgcagcta agaacacagc aattgcagcg
     1141 gttgataaag cgacaacagc agatggtgtt aaccaagaac ttggtaaagg catcacagct
     1201 atcaacaaag cttaccgtcc aggtgaagct gttaaagcac gtaaagaagc cgctaaagct
     1261 gatcttgaaa aagaagctgc taaagtgaaa gctcttattg ctaaggatcc gaccttaact
     1321 caagctgcta aagacaaaca aacagcagcg gttgacgcag ctaagaatac agcaattg
//
LOCUS       DATXIG010000125         1369 bp    DNA     linear   BCT 28-DEC-2023
DEFINITION  TPA_asm: Streptococcus pyogenes strain MGAS11113
            SAMN08555799-rid19682583.denovo.125, whole genome shotgun sequence.
ACCESSION   DATXIG010000125 DATXIG010000000
VERSION     DATXIG010000125.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN08555799
            Sequence Read Archive: SRR6740495
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pyogenes
  ORGANISM  Streptococcus pyogenes
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 1369)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1369)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-OCT-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-OCT-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001918058.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001918058.2
            Genome Coverage       :: 368x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/12/2023 11:23:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 1,806
            CDSs (total)                      :: 1,769
            Genes (coding)                    :: 1,722
            CDSs (with protein)               :: 1,722
            Genes (RNA)                       :: 37
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1, 1 (5S, 23S)
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 47
            CDSs (without protein)            :: 47
            Pseudo Genes (ambiguous residues) :: 0 of 47
            Pseudo Genes (frameshifted)       :: 30 of 47
            Pseudo Genes (incomplete)         :: 20 of 47
            Pseudo Genes (internal stop)      :: 9 of 47
            Pseudo Genes (multiple problems)  :: 11 of 47
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1369
                     /organism="Streptococcus pyogenes"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN08555799-rid19682583.denovo.125"
                     /strain="MGAS11113"
                     /serotype="Emm28"
                     /isolation_source="normally sterile site"
                     /host="Homo sapiens"
                     /db_xref="taxon:1314"
                     /country="Finland"
                     /collection_date="2002"
                     /collected_by="National Public Health Laboratory"
     gene            204..320
                     /locus_tag="VC122_001721"
                     /pseudo
     CDS             204..320
                     /locus_tag="VC122_001721"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611995.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS3 family transposase"
     gene            complement(506..>1369)
                     /locus_tag="VC122_001722"
     CDS             complement(506..>1369)
                     /locus_tag="VC122_001722"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011285469.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1542 domain-containing protein"
                     /protein_id="HEP5641619.1"
                     /translation="ADLEREAAKVREAIANDPTLTKADKAKQTEAVAKALKAAIAAVD
                     KATTAEGINQELGKGITAINKAYRPGEGVEAHKEAAKANLEKVAKETKALISGDRYLS
                     ETEKAVQKQAVEQALAKALGQVEAAKTVEAVKLAENLGTVAIRSAYVAGLAKDTDQAT
                     AALNEAKQAAIEALKQAAAETLAKITTDAKLTEAQKAEQSENVSLALKTAIATVRSAQ
                     SIASVKEAIDKGITAIRAAYVPNKAVAKSSSANHLPKSGDANSIVLVGLGVMSLLLGM
                     VLYSKKKESKD"
BASE COUNT          385 a          289 c          244 g          451 t
ORIGIN      
        1 agatttcatg tagaaattag taaaagttag gagatttttt ggtcgtttca aacgataaca
       61 tcatcttctt ataacaagaa cttcactcag agataaacta ttctaatact ttagtagcct
      121 cttttgagct tgtagcacga aaattcgctc gaactgattt aaaataggca ttttatctgc
      181 tcgcttgatc cctactgcgc gaccaagaaa ccttttattc actagaacaa ttaaccctta
      241 aaaccaagga ggaagtccac tggtggaact accatcacct tcacagtaat cttaattatc
      301 aaacacccat aactaaatga gtcatcgttt gaacaaaaaa cgccttataa attctgtaca
      361 gaaaactgtt gccttttcaa ttgaactttg aaatgttttc aatacactcc gttcttgggc
      421 ccttacgatc caaaaaacca aggtgatttc aaaatcacct tggtttgaaa atatagtaga
      481 aagcaatttg gataaagcca ggttcttagt ctttactttc ttttttcttg ctataaagca
      541 ccatacctaa aagaagagac ataactccta agccaacaag aacaattgag tttgcatcac
      601 ctgattttgg aagatggttc gctgacgatg attttgcgac tgccttatta ggcacatagg
      661 ctgcacggat agcagtaata cctttatcta ttgcttcttt cacagacgca atagattgtg
      721 ctgaacgaac agtcgcaata gccgtcttaa gcgctaatga tacattttct gattgttcag
      781 ctttttgagc ttcagtcaat ttagcatcag ttgtaatctt agcaagtgtt tctgccgcag
      841 cttgtttaag agcttcaata gcagcttgtt tcgcttcgtt aagagcagct gttgcttgat
      901 cagtatcttt agctaaacca gcaacatatg ctgaacggat agctacagta ccaaggtttt
      961 ctgccaactt aacagcttca actgtcttag cagcctcaac ttgaccaagt gctttcgcaa
     1021 gagcttgctc aacagcttgt ttttggactg ctttttcagt ttcgctcaag taacggtctc
     1081 ctgaaataag agctttagtt tctttagcta ctttttcaag attagcttta gcagcttctt
     1141 tatgtgcttc aacaccttca cctggacggt aagctttgtt gatagctgtg atgcctttac
     1201 caagttcttg gttaatgcct tcagctgttg tcgctttatc aaccgctgcg atagcagctt
     1261 taagagcttt agcaacagct tctgtttgtt tagctttatc agcttttgtt aaggttgggt
     1321 cgttagcgat agcttcacga accttagcag cttctctttc aagatcagc
//
LOCUS       DATXIG010000126         1353 bp    DNA     linear   BCT 28-DEC-2023
DEFINITION  TPA_asm: Streptococcus pyogenes strain MGAS11113
            SAMN08555799-rid19682583.denovo.126, whole genome shotgun sequence.
ACCESSION   DATXIG010000126 DATXIG010000000
VERSION     DATXIG010000126.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN08555799
            Sequence Read Archive: SRR6740495
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pyogenes
  ORGANISM  Streptococcus pyogenes
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 1353)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1353)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-OCT-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-OCT-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001918058.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001918058.2
            Genome Coverage       :: 368x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/12/2023 11:23:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 1,806
            CDSs (total)                      :: 1,769
            Genes (coding)                    :: 1,722
            CDSs (with protein)               :: 1,722
            Genes (RNA)                       :: 37
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1, 1 (5S, 23S)
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 47
            CDSs (without protein)            :: 47
            Pseudo Genes (ambiguous residues) :: 0 of 47
            Pseudo Genes (frameshifted)       :: 30 of 47
            Pseudo Genes (incomplete)         :: 20 of 47
            Pseudo Genes (internal stop)      :: 9 of 47
            Pseudo Genes (multiple problems)  :: 11 of 47
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1353
                     /organism="Streptococcus pyogenes"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN08555799-rid19682583.denovo.126"
                     /strain="MGAS11113"
                     /serotype="Emm28"
                     /isolation_source="normally sterile site"
                     /host="Homo sapiens"
                     /db_xref="taxon:1314"
                     /country="Finland"
                     /collection_date="2002"
                     /collected_by="National Public Health Laboratory"
     gene            128..502
                     /locus_tag="VC122_001723"
     CDS             128..502
                     /locus_tag="VC122_001723"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005957761.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEP5641620.1"
                     /translation="MSCIMSQMNTGKIVFPSILAMMLLQSIVASIASLIFPAGLIGAK
                     VTEKLYTGKSYIVFLVFSSIIPAIYFTAIMSISGLLRMKGYDENFWKIYFSSFPINVV
                     FGYFSSIFWNVILDKFLRRKEA"
     gene            504..929
                     /locus_tag="VC122_001724"
     CDS             504..929
                     /locus_tag="VC122_001724"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002840511.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family transcriptional regulator"
                     /protein_id="HEP5641621.1"
                     /translation="MNKSIGKLITYLSRCIQQELKNVVVPFDISVGEEPYYMALTKQD
                     GLTQDELTKLVKVDKAATTRMVKSLEQKGYITRETDPKDKRNKKIYLTERGRLKYEPL
                     SRALKEFNQKLTSEWTDEQYKIVYQSLEMLQKKFEIDNK"
     gene            1204..1302
                     /locus_tag="VC122_001725"
                     /pseudo
     CDS             1204..1302
                     /locus_tag="VC122_001725"
                     /inference="COORDINATES: protein motif:HMM:NF024729.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS3 family transposase"
BASE COUNT          524 a          176 c          239 g          414 t
ORIGIN      
        1 atctaagctt aaaaacaaat tagttgacgc atcaactaat ttgtgatata cttaagttat
       61 tcttaaaagt gaggtaagag taatgaaata taaacgttat attttcccta tattgatggg
      121 gagtacaatg agttgtatta tgtctcagat gaatactgga aaaattgttt ttccgtctat
      181 acttgcaatg atgttattac aatctatagt tgcaagtata gcatctttga tttttccggc
      241 tggacttata ggtgcaaaag taacagagaa actatatact ggaaaatcat atattgtttt
      301 tttagttttt tcatcaataa ttccagcgat atattttact gcaattatgt ctataagcgg
      361 attgttaaga atgaagggat atgatgagaa tttctggaaa atatattttt cctcatttcc
      421 tattaatgtt gtatttggat atttttctag tattttttgg aatgtgatat tggacaaatt
      481 tttaagaaga aaagaggcat aaagtgaaca aaagcattgg aaaacttata acttatttat
      541 caagatgtat tcagcaagaa ttaaaaaatg tagttgttcc atttgatata agtgtcggag
      601 aagagcctta ttacatggca cttacaaaac aggatggatt gactcaggat gaattaacaa
      661 aacttgtgaa agtagataag gctgctacga ctcgaatggt taaatcatta gagcaaaagg
      721 gatatatcac acgagaaaca gaccctaaag ataagagaaa caaaaaaata tatcttacag
      781 aaaggggacg attaaaatat gaacctttaa gtagagcatt aaaagaattt aatcagaagt
      841 taacatcaga atggacagat gaacaatata aaatcgttta tcaaagtctg gaaatgctac
      901 aaaaaaaatt tgagattgat aataaataag ttttaattta ttgcccctct acacatcggg
      961 tcaacttgcc acgaactttc acaactaaat acccaccgct aaaacagcgg cacaccaagc
     1021 aggaaatctg aaaaaggtct cctggtatgg atacaataaa taataagtaa tcataggagg
     1081 attttaaatt agctagacac tacgaagaag acttcaaaag gcaaatagta gaaatcttta
     1141 atcaaggtaa caacacatat aaaaaattgg gagaagaata cgatatagca ccgtcaataa
     1201 tcagagtatg tttattggaa caataaccta agggttaatg gatcattagg ttacttgaca
     1261 ccagttaaat ataggcaatc aagattaact agtagttatt agttaatcta aaattagtac
     1321 gttcgatttt agattgtaca aataggtgtt gac
//
LOCUS       DATXIG010000127         1293 bp    DNA     linear   BCT 28-DEC-2023
DEFINITION  TPA_asm: Streptococcus pyogenes strain MGAS11113
            SAMN08555799-rid19682583.denovo.127, whole genome shotgun sequence.
ACCESSION   DATXIG010000127 DATXIG010000000
VERSION     DATXIG010000127.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN08555799
            Sequence Read Archive: SRR6740495
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pyogenes
  ORGANISM  Streptococcus pyogenes
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 1293)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1293)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-OCT-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-OCT-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001918058.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001918058.2
            Genome Coverage       :: 368x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/12/2023 11:23:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 1,806
            CDSs (total)                      :: 1,769
            Genes (coding)                    :: 1,722
            CDSs (with protein)               :: 1,722
            Genes (RNA)                       :: 37
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1, 1 (5S, 23S)
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 47
            CDSs (without protein)            :: 47
            Pseudo Genes (ambiguous residues) :: 0 of 47
            Pseudo Genes (frameshifted)       :: 30 of 47
            Pseudo Genes (incomplete)         :: 20 of 47
            Pseudo Genes (internal stop)      :: 9 of 47
            Pseudo Genes (multiple problems)  :: 11 of 47
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1293
                     /organism="Streptococcus pyogenes"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN08555799-rid19682583.denovo.127"
                     /strain="MGAS11113"
                     /serotype="Emm28"
                     /isolation_source="normally sterile site"
                     /host="Homo sapiens"
                     /db_xref="taxon:1314"
                     /country="Finland"
                     /collection_date="2002"
                     /collected_by="National Public Health Laboratory"
     gene            complement(<1..103)
                     /locus_tag="VC122_001726"
     CDS             complement(<1..103)
                     /locus_tag="VC122_001726"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001059491.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="toxin-antitoxin system, toxin component, MazF
                     family protein"
                     /protein_id="HEP5641622.1"
                     /translation="MSDIEVYSVLVSRIEYSDGTGSKVRPAVVVKFND"
     gene            complement(100..393)
                     /locus_tag="VC122_001727"
     CDS             complement(100..393)
                     /locus_tag="VC122_001727"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000108708.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelB/DinJ family
                     antitoxin"
                     /protein_id="HEP5641623.1"
                     /translation="MSTVAVRVDDQLKDDATELFQSLGLDMSTAVKMFLIQSVKTQSI
                     PFEIKNQSSVSDEEFQNLVETKLKGIRVKASDPESVNAFFGDEDFSEYEEYFK"
     gene            450..599
                     /locus_tag="VC122_001728"
                     /pseudo
     CDS             450..599
                     /locus_tag="VC122_001728"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000191693.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS256 family transposase"
     gene            complement(1105..>1293)
                     /locus_tag="VC122_001729"
     CDS             complement(1105..>1293)
                     /locus_tag="VC122_001729"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_115075552.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LPXTG cell wall anchor domain-containing
                     protein"
                     /protein_id="HEP5641624.1"
                     /translation="VKVVDPRTDADKNDPAGKNQQVKGKGNKLPATGENATPFFNVAA
                     LTIISSVGLLSVSKKKED"
BASE COUNT          455 a          243 c          168 g          427 t
ORIGIN      
        1 catcattaaa tttgactact acagcaggac gaacctttga acctgtacca tcagaatatt
       61 caatacgtga aactaataca gaatatacct caatatcact catttaaaat attcctcata
      121 ttctgaaaaa tcttcatctc caaaaaacgc atttacactt tctggatcac tagccttaac
      181 tcgtattcct tttaattttg tttctaccaa attttgaaat tcttcatcag aaacagatga
      241 ttgatttttt atttcaaaag gaatactttg agttttaaca gactgaatca aaaacatttt
      301 tactgctgta ctcatatcca accctaagga ttgaaaaagt tctgtagcat catcttttaa
      361 ttgatcatca actcttactg ctactgttga cataatatca cctcaaatca tttttgtata
      421 accattgtat cactaactgc aatacaacgc aaacaaaaaa gaaaatcgta tttcccagtg
      481 aagagaccct agaacactgt ctagtcacac tatttaatga ttacaacttc aagcaagaac
      541 aacgagtcca caaaggtttt ggtcattgta cagacacact tgaaagccta tttgattaac
      601 aatcccagct ttgccagtgt gtttacacaa cattattgac acactccttt tatttttata
      661 aattcccctt aaatacattt aattacttga aaagacaaac agtcatttta gaaaattttg
      721 catctaacct tacatttgat tacacctaaa aattttaaat caacaaaaga gagcctagga
      781 ttaacatctt aggctctaat gatacacttc atttacaaat ttcaactaaa taggatcatt
      841 tcttcagttt tatagtaagc gtccaataac aaggtatatt ggatgcttac ggaataggga
      901 agaaaatgga agatacctcg ttattggacg cttacacaaa atcaaaagcc tatcgaaatt
      961 aataatctcg ataggctttt tcaagaatta aactcgaaaa attgaaatac tttaattaag
     1021 tttgttggag agagatacat aactttatta atgtgttcac accaataaat ggtgaaaagt
     1081 ttagtgacat tttaggtcaa aagattaatc ctcttttttc ttagaaacag ataataaacc
     1141 aactgatgat ataattgtca aagctgcaac attaaagaat ggagttgcat tctcacctgt
     1201 tgctggtagt ttatttcctt tacctttgac ttgctgattt ttacctgctg gatcattctt
     1261 atcggcatct gtacgtggat cgacaacctt aac
//
LOCUS       DATXIG010000128         1281 bp    DNA     linear   BCT 28-DEC-2023
DEFINITION  TPA_asm: Streptococcus pyogenes strain MGAS11113
            SAMN08555799-rid19682583.denovo.128, whole genome shotgun sequence.
ACCESSION   DATXIG010000128 DATXIG010000000
VERSION     DATXIG010000128.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN08555799
            Sequence Read Archive: SRR6740495
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pyogenes
  ORGANISM  Streptococcus pyogenes
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 1281)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1281)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-OCT-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-OCT-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001918058.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001918058.2
            Genome Coverage       :: 368x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/12/2023 11:23:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 1,806
            CDSs (total)                      :: 1,769
            Genes (coding)                    :: 1,722
            CDSs (with protein)               :: 1,722
            Genes (RNA)                       :: 37
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1, 1 (5S, 23S)
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 47
            CDSs (without protein)            :: 47
            Pseudo Genes (ambiguous residues) :: 0 of 47
            Pseudo Genes (frameshifted)       :: 30 of 47
            Pseudo Genes (incomplete)         :: 20 of 47
            Pseudo Genes (internal stop)      :: 9 of 47
            Pseudo Genes (multiple problems)  :: 11 of 47
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1281
                     /organism="Streptococcus pyogenes"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN08555799-rid19682583.denovo.128"
                     /strain="MGAS11113"
                     /serotype="Emm28"
                     /isolation_source="normally sterile site"
                     /host="Homo sapiens"
                     /db_xref="taxon:1314"
                     /country="Finland"
                     /collection_date="2002"
                     /collected_by="National Public Health Laboratory"
     gene            12..>1281
                     /gene="spr28"
                     /locus_tag="VC122_001730"
     CDS             12..>1281
                     /gene="spr28"
                     /locus_tag="VC122_001730"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_115157939.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adhesin R28"
                     /protein_id="HEP5641625.1"
                     /translation="MFRRSKNNSYDTSQTKQRFSIKKFKFGAASVLIGLSFLGGVTQG
                     NLNIFEESIVAASTIPGSAATLNTSITKNIQNGNAYIDLYDVKNGLIDPQNLIVLNPS
                     SYSANYYIKQGAKYYSNPSEITTTGSATITFNILDETGNPHKKADGQIDIVSVNLTIY
                     DSTALRNRIDEVINNANDPKWSDGSRDEVLTGLEKIKKDIDNNPKTQIDIDNKINEVN
                     EIEKLLVVSLPDKIKYSPEAKHRTVEQHAELDAKDSIANTDELPSNSTYNWKNGHKPD
                     TSTSGEKDGIVEVHYPDGTVDDVNVKVTVTSKKTDNTAPTLTVTPEQQTVKVDEDITF
                     TVTVEDENEVELGLDDLKAKYENDIIGARVKIKYLTKEPNKKVMEVTIMKATLADKGA
                     ITFTAKDKAGNQAEPKTVTINVLPLKDSNEP"
BASE COUNT          506 a          184 c          237 g          354 t
ORIGIN      
        1 aggagaatat tatgtttaga aggtctaaaa ataacagtta tgatacttca cagacgaaac
       61 aacggttttc aattaagaag ttcaagtttg gtgcagcttc tgtactaatt ggtcttagtt
      121 ttttgggtgg ggttacacaa ggtaatctta atatttttga agagtcaata gttgctgcat
      181 ctacaattcc agggagtgca gcgaccttaa atacaagcat cactaaaaat atacaaaacg
      241 gaaatgctta catagattta tatgatgtaa agaatggatt gattgatcct caaaacctca
      301 ttgtattaaa tccatcaagc tattcagcaa attattatat caaacaaggt gctaaatatt
      361 atagtaatcc gagtgaaatt acaacaactg gttcagcaac tattactttt aatatacttg
      421 atgaaactgg aaatccacat aaaaaagctg atggacaaat tgatatagtt agtgtgaatt
      481 taactatata tgattctaca gctttaagaa ataggataga tgaagtaata aataatgcaa
      541 atgatcctaa gtggagtgat gggagtcgtg atgaagtctt aactggatta gaaaaaataa
      601 aaaaagatat tgataataat ccaaaaacac aaatagatat tgataataaa attaatgaag
      661 tcaatgaaat agagaaattg ttagttgtat cgctaccaga taaaattaag tattcgccag
      721 aggctaagca taggactgtt gaacaacacg cggaattaga tgcaaaagat agcattgcaa
      781 atacagatga attgccatca aattcaacgt ataactggaa aaatggtcat aaaccagaca
      841 cctcaacatc aggtgaaaaa gacggaattg ttgaagttca ctatccagat ggtactgttg
      901 atgatgtgaa tgttaaagta accgttacat cgaaaaaaac tgataataca gctccaacat
      961 taaccgtcac tccagagcaa cagactgtta aagtggatga agatattacc tttacggtta
     1021 cagttgaaga cgaaaatgaa gttgaactag gtttagatga tcttaaagct aagtatgaaa
     1081 atgatatcat tggagctcgt gttaaaatta agtatcttac taaagaacct aataagaaag
     1141 tcatggaagt gacaattatg aaagctactt tagcagataa gggcgcaatt acctttactg
     1201 caaaagataa agcaggtaat caagcagaac ctaagacagt taccatcaat gttcttccgc
     1261 ttaaggatag caacgaacca a
//
LOCUS       DATXIG010000129         1265 bp    DNA     linear   BCT 28-DEC-2023
DEFINITION  TPA_asm: Streptococcus pyogenes strain MGAS11113
            SAMN08555799-rid19682583.denovo.129, whole genome shotgun sequence.
ACCESSION   DATXIG010000129 DATXIG010000000
VERSION     DATXIG010000129.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN08555799
            Sequence Read Archive: SRR6740495
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pyogenes
  ORGANISM  Streptococcus pyogenes
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 1265)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1265)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-OCT-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-OCT-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001918058.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001918058.2
            Genome Coverage       :: 368x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/12/2023 11:23:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 1,806
            CDSs (total)                      :: 1,769
            Genes (coding)                    :: 1,722
            CDSs (with protein)               :: 1,722
            Genes (RNA)                       :: 37
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1, 1 (5S, 23S)
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 47
            CDSs (without protein)            :: 47
            Pseudo Genes (ambiguous residues) :: 0 of 47
            Pseudo Genes (frameshifted)       :: 30 of 47
            Pseudo Genes (incomplete)         :: 20 of 47
            Pseudo Genes (internal stop)      :: 9 of 47
            Pseudo Genes (multiple problems)  :: 11 of 47
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1265
                     /organism="Streptococcus pyogenes"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN08555799-rid19682583.denovo.129"
                     /strain="MGAS11113"
                     /serotype="Emm28"
                     /isolation_source="normally sterile site"
                     /host="Homo sapiens"
                     /db_xref="taxon:1314"
                     /country="Finland"
                     /collection_date="2002"
                     /collected_by="National Public Health Laboratory"
     gene            <1..957
                     /locus_tag="VC122_001731"
     CDS             <1..957
                     /locus_tag="VC122_001731"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011528608.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing histidine kinase"
                     /protein_id="HEP5641626.1"
                     /translation="PEFSNHYLRNVSYNMLTFYILGIGTSISIVVALTRFVKEISLNF
                     KEIKKLANKMGIEVLSENENYSKIIEFDDILRTLHIKGDNLKSLIEREILEKQDLSFQ
                     IAALSHDIKTPLTVLKGNLELLELTTLNKNQEGYIVSMNNSISVFEGYFNSLISYTRM
                     LSEDRSVKLILVEKLLSELHFEVDDLLNINNIEFSICNRLIITSFYGDEENLIRALSN
                     LLVNAIRFMPVLDKKIEVILSESGEQIHFEIWNNGERFSDSTLKKGDKLFYTEDYSRG
                     NKHYGIGLAFVKGVAIKHGGNLQLNNPARGGASAIISIKKKI"
     gene            1114..1254
                     /locus_tag="VC122_001732"
     CDS             1114..1254
                     /locus_tag="VC122_001732"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011528609.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lantibiotic streptin"
                     /protein_id="HEP5641627.1"
                     /translation="MNNTIKDFDLDLKTNKKDTATPYVGSRYLCTPGSCWKLVCFTTT
                     VK"
BASE COUNT          457 a          157 c          229 g          422 t
ORIGIN      
        1 ccggagtttt ctaaccatta ccttagaaat gtttcatata acatgttgac attttatatt
       61 ctgggaatag gaacgagtat cagcattgta gttgctttaa cacggtttgt aaaagaaatt
      121 tctttgaatt tcaaggaaat caagaagtta gcaaataaaa tggggataga agtcctttct
      181 gaaaatgaaa attattctaa aataatcgag tttgatgata tacttagaac tttacatatt
      241 aaaggtgata atttgaaaag tttgattgag cgagaaatat tggaaaagca agatctatcc
      301 tttcaaattg cagcactttc gcatgatata aaaacacctt taacggtttt gaaaggaaat
      361 cttgagttat tagaattgac tactttaaat aaaaatcagg aaggttatat tgtatctatg
      421 aacaatagta tttctgtttt tgaaggctat ttcaattctt taatatctta tacacgcatg
      481 ctttcagagg atagatcagt gaaactaatt ttagttgaga agctattaag tgagttacat
      541 tttgaagtcg atgacttatt gaatatcaat aatattgaat tttctatttg taatagactt
      601 attatcactt ctttttatgg agatgaggaa aatctgataa gagctctttc taacctttta
      661 gttaatgcaa taagatttat gcccgtatta gataaaaaaa tcgaagttat actgtctgag
      721 tcaggagagc aaattcattt tgaaatatgg aataatggtg agcgtttcag tgatagtaca
      781 ctgaaaaagg gagataaatt attttacacg gaagattata gcagaggtaa caaacattat
      841 ggaatcggtt tggcatttgt taaaggcgtt gctatcaaac acggtgggaa tcttcaattg
      901 aataatccag ctagaggagg tgctagcgcc ataatctcga taaaaaagaa aatttaatag
      961 atttgattga aactggagta tttcaggcaa aaaatcgtaa atttttgtga tttttggcta
     1021 aaatactttt tttctgaaca aattctgaac ttggggtgtc ataataatca tgtaaatata
     1081 tcggatgcaa aaacgtaaga gaggaactag aaaatgaata acacaattaa agactttgat
     1141 ctcgatttga aaacaaataa aaaagacact gctacacctt atgttggtag ccgttaccta
     1201 tgtacccctg gttcttgttg gaaattagtt tgctttacaa caactgttaa ataataaagt
     1261 tatca
//
LOCUS       DATXIG010000130         1263 bp    DNA     linear   BCT 28-DEC-2023
DEFINITION  TPA_asm: Streptococcus pyogenes strain MGAS11113
            SAMN08555799-rid19682583.denovo.130, whole genome shotgun sequence.
ACCESSION   DATXIG010000130 DATXIG010000000
VERSION     DATXIG010000130.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN08555799
            Sequence Read Archive: SRR6740495
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pyogenes
  ORGANISM  Streptococcus pyogenes
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 1263)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1263)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-OCT-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-OCT-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001918058.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001918058.2
            Genome Coverage       :: 368x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/12/2023 11:23:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 1,806
            CDSs (total)                      :: 1,769
            Genes (coding)                    :: 1,722
            CDSs (with protein)               :: 1,722
            Genes (RNA)                       :: 37
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1, 1 (5S, 23S)
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 47
            CDSs (without protein)            :: 47
            Pseudo Genes (ambiguous residues) :: 0 of 47
            Pseudo Genes (frameshifted)       :: 30 of 47
            Pseudo Genes (incomplete)         :: 20 of 47
            Pseudo Genes (internal stop)      :: 9 of 47
            Pseudo Genes (multiple problems)  :: 11 of 47
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1263
                     /organism="Streptococcus pyogenes"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN08555799-rid19682583.denovo.130"
                     /strain="MGAS11113"
                     /serotype="Emm28"
                     /isolation_source="normally sterile site"
                     /host="Homo sapiens"
                     /db_xref="taxon:1314"
                     /country="Finland"
                     /collection_date="2002"
                     /collected_by="National Public Health Laboratory"
     gene            121..309
                     /locus_tag="VC122_001733"
     CDS             121..309
                     /locus_tag="VC122_001733"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011285321.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HEP5641628.1"
                     /translation="MLITENIAVKVRKKRAVKQLGKVELSKKLGITPPTLSKIEAGNY
                     KAPKRIYASVMEWLAEDY"
     gene            325..891
                     /locus_tag="VC122_001734"
     CDS             325..891
                     /locus_tag="VC122_001734"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011285320.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage repressor protein"
                     /protein_id="HEP5641629.1"
                     /translation="MNRQVSIFEEVNDQGGAFQVVTTMTFHDRLLDIYGNIQEPLFRA
                     RDVAEMIDHSKTRNGYYDVQSMLRKVDDDEKVKGVPLEGATKNFRSGQKVWFLTEHGL
                     YEVLMRSSKPKAKEFRKAVKNILKEIRLNGYYMQGELVQNSQPTPDIDDLSYIKQKLT
                     DLQDVDSLQDLRWKMAKLYHVIETLDEY"
     gene            948..1190
                     /locus_tag="VC122_001735"
     CDS             948..1190
                     /locus_tag="VC122_001735"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000648623.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage antirepressor protein"
                     /protein_id="HEP5641630.1"
                     /translation="MKATTYKELKKWIDEGVDLAELAQGYADKVPSVDREQFEAVTQG
                     IFNVLEGVSLMLDDKVLIYNRKAEQKRLNDIEQGDY"
BASE COUNT          484 a          192 c          271 g          316 t
ORIGIN      
        1 aaaaaagcat aagacgaaaa aaaattggat ttttatcttg acaaccaaat ttatttgcgt
       61 taatatagat tttgcaaaaa taagacgaaa aaaaattggg tttttgcaaa ggaggaatat
      121 atgcttatta cagaaaatat agctgttaag gttcgtaaaa aaagagctgt taaacagtta
      181 ggaaaagtgg agctttctaa aaaattaggg ataacgccac ccactctatc aaaaattgaa
      241 gctggcaact acaaagcacc taagcgtatc tacgctagtg tcatggaatg gctagcagag
      301 gattattaga aaggcaggaa caaaatgaat agacaggtca gtatttttga agaagtcaat
      361 gatcaagggg gagctttcca agttgtaacc actatgactt ttcacgatcg gctattagat
      421 atttacggta atattcaaga accgttattt agagctagag atgttgcgga aatgattgat
      481 cacagcaaaa caaggaacgg atattatgac gttcaatcta tgctaagaaa ggtagatgat
      541 gacgaaaaag ttaaaggcgt ccctttagag ggtgctacga aaaatttccg tagcggtcaa
      601 aaagtttggt ttttaactga gcatggactt tatgaagtgc ttatgcggtc aagcaaacca
      661 aaagccaaag agtttagaaa agcagtcaaa aacattctca aagaaatccg tttgaatggc
      721 tactacatgc aaggagagct tgtccaaaac agtcaaccga ccccagacat tgatgattta
      781 agctatatca agcagaagtt aactgactta caggacgttg acagcttaca agatttacgg
      841 tggaagatgg ctaagttata tcacgtcatc gaaacactgg atgaatatta agaaataaga
      901 caacaaaaaa aagcgcaata atacgcacgg ataaaggaga aatgattatg aaagcaacaa
      961 cttacaaaga gttgaagaaa tggattgatg aaggtgttga tttagctgag ctagcacagg
     1021 gttacgctga caaagtacct agtgtagacc gtgaacagtt tgaagcagtc acacagggaa
     1081 ttttcaacgt attggaaggc gtatcgctca tgcttgatga caaagtgcta atctataatc
     1141 gcaaagcaga gcaaaagcgt ttgaatgaca ttgaacaagg tgattattaa ttaataatca
     1201 agagtaacaa aaaaagcctt gaactttggc gaggaacagg cttttcactt caattaacta
     1261 aaa
//
LOCUS       DATXIG010000131         1237 bp    DNA     linear   BCT 28-DEC-2023
DEFINITION  TPA_asm: Streptococcus pyogenes strain MGAS11113
            SAMN08555799-rid19682583.denovo.131, whole genome shotgun sequence.
ACCESSION   DATXIG010000131 DATXIG010000000
VERSION     DATXIG010000131.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN08555799
            Sequence Read Archive: SRR6740495
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pyogenes
  ORGANISM  Streptococcus pyogenes
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 1237)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1237)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-OCT-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-OCT-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001918058.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001918058.2
            Genome Coverage       :: 368x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/12/2023 11:23:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 1,806
            CDSs (total)                      :: 1,769
            Genes (coding)                    :: 1,722
            CDSs (with protein)               :: 1,722
            Genes (RNA)                       :: 37
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1, 1 (5S, 23S)
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 47
            CDSs (without protein)            :: 47
            Pseudo Genes (ambiguous residues) :: 0 of 47
            Pseudo Genes (frameshifted)       :: 30 of 47
            Pseudo Genes (incomplete)         :: 20 of 47
            Pseudo Genes (internal stop)      :: 9 of 47
            Pseudo Genes (multiple problems)  :: 11 of 47
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1237
                     /organism="Streptococcus pyogenes"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN08555799-rid19682583.denovo.131"
                     /strain="MGAS11113"
                     /serotype="Emm28"
                     /isolation_source="normally sterile site"
                     /host="Homo sapiens"
                     /db_xref="taxon:1314"
                     /country="Finland"
                     /collection_date="2002"
                     /collected_by="National Public Health Laboratory"
     gene            <1..1227
                     /locus_tag="VC122_001736"
     CDS             <1..1227
                     /locus_tag="VC122_001736"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002992394.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="insulinase family protein"
                     /protein_id="HEP5641631.1"
                     /translation="ENGLTVYFIKKIGFLEKTAMLTVGFGSLDNKLTVDDESRDAPAG
                     IAHFLEHKLFEDESGGDISLKFTQLGAETNAFTTFNQTSFFFSTASKFQENLELLQYF
                     VLSANITDESVSREKKIIGQEIDMYQDDADYRAYSGILQNLFPKTSLANDIAGSKASI
                     QKITKILLETHHTYFYQPTNMSLFIVGDIDIDETFLAIQRFQTTLSYPDRKRVTVDPL
                     HYYPVIKSSSVDMDVTIAKLVVGFRGYLTLTQHSLLTYRIALKLFLSMLIGWTSKIYH
                     TLYEDGKIDDSFDVDVEIHHHFQFVLISLDTPEPIAMSNYIRQKLATIKISKEFTNEH
                     LNLLKKEMYGDFIQSLDSIEHLTHQFSLYLSDSDKETYFDIPKIIERLTLKDVVTIGK
                     AFFEKADASDFTVFPK"
BASE COUNT          428 a          204 c          197 g          408 t
ORIGIN      
        1 gaaaatggtt taactgttta ttttataaaa aaaatagggt ttttagaaaa aacagctatg
       61 ttgacagtag gtttcggttc tcttgacaat aagttgacag tcgatgacga atcacgagat
      121 gctccagcag gtattgcaca ctttttagaa cataaactct ttgaagatga atcaggtgga
      181 gatatttcat taaagtttac acagctagga gcagagacaa atgcctttac aactttcaac
      241 caaacgagtt tcttcttttc aacagctagt aaatttcagg aaaatttgga actccttcaa
      301 tattttgttc tttctgctaa tataacagat gaatctgtta gtagagaaaa aaaaataatc
      361 gggcaagaaa ttgatatgta tcaagatgat gctgactacc gtgcatatag tggaatcctt
      421 cagaacttat ttccaaaaac aagtttagct aatgatattg ctggctcaaa agcatccatt
      481 caaaagatca cgaaaatatt attggaaacg catcatactt atttttacca acctactaat
      541 atgagtctat tcattgtcgg tgatattgat attgacgaaa cttttttagc tattcaaagg
      601 tttcaaacca cattatccta tccagataga aaaagggtaa ctgttgatcc tttacactac
      661 tatcctgtca ttaaatcatc ttctgttgac atggatgtta ctattgccaa gctggttgtt
      721 ggttttcgag ggtatctgac cttaactcag cattcactat taacgtatcg tattgccttg
      781 aagctatttt tatcaatgct tataggttgg acttctaaaa tctatcatac gctatatgaa
      841 gatggtaaaa tagacgattc atttgatgtg gatgttgaaa ttcatcatca ttttcaattt
      901 gtattaatat cacttgatac accagagcct atcgcaatgt ctaactatat ccgtcaaaaa
      961 ttagccacta taaagatatc aaaagagttt acaaatgagc acttaaatct attaaaaaaa
     1021 gaaatgtatg gtgactttat tcagagttta gactctatag aacatttaac ccatcagttc
     1081 agtctatacc tgtctgattc tgataaagaa acatattttg atattccaaa aattatagaa
     1141 agactcactc taaaagatgt tgttacaatt ggaaaagcat tttttgaaaa agctgatgct
     1201 tcagacttca cagtcttccc aaaatagatg aaaatct
//
LOCUS       DATXIG010000132         1211 bp    DNA     linear   BCT 28-DEC-2023
DEFINITION  TPA_asm: Streptococcus pyogenes strain MGAS11113
            SAMN08555799-rid19682583.denovo.132, whole genome shotgun sequence.
ACCESSION   DATXIG010000132 DATXIG010000000
VERSION     DATXIG010000132.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN08555799
            Sequence Read Archive: SRR6740495
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pyogenes
  ORGANISM  Streptococcus pyogenes
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 1211)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1211)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-OCT-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-OCT-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001918058.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001918058.2
            Genome Coverage       :: 368x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/12/2023 11:23:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 1,806
            CDSs (total)                      :: 1,769
            Genes (coding)                    :: 1,722
            CDSs (with protein)               :: 1,722
            Genes (RNA)                       :: 37
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1, 1 (5S, 23S)
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 47
            CDSs (without protein)            :: 47
            Pseudo Genes (ambiguous residues) :: 0 of 47
            Pseudo Genes (frameshifted)       :: 30 of 47
            Pseudo Genes (incomplete)         :: 20 of 47
            Pseudo Genes (internal stop)      :: 9 of 47
            Pseudo Genes (multiple problems)  :: 11 of 47
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1211
                     /organism="Streptococcus pyogenes"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN08555799-rid19682583.denovo.132"
                     /strain="MGAS11113"
                     /serotype="Emm28"
                     /isolation_source="normally sterile site"
                     /host="Homo sapiens"
                     /db_xref="taxon:1314"
                     /country="Finland"
                     /collection_date="2002"
                     /collected_by="National Public Health Laboratory"
     gene            complement(104..1018)
                     /gene="hasC"
                     /locus_tag="VC122_001737"
     CDS             complement(104..1018)
                     /gene="hasC"
                     /locus_tag="VC122_001737"
                     /EC_number="2.7.7.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011018342.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UTP--glucose-1-phosphate uridylyltransferase
                     HasC"
                     /protein_id="HEP5641632.1"
                     /translation="MTKVRKAIIPAAGLGTRFLPATKALAKEMLPIVDKPTIQFIVEE
                     ALKSGIEEILVVTGKAKRSIEDHFDSNFELEYNLQAKGKNELLKLVDETTAINLHFIR
                     QSHPRGLGDAVLQAKAFVGNEPFVVMLGDDLMDITNASAKPLTKQLMEDYDKTHASTI
                     AVMKVPHEDVSSYGVIAPQGKAVKGLYSVDTFVEKPQPEDAPSDLAIIGRYLLTPEIF
                     DILERQTPGAGNEVQLTDAIDTLNKTQRVFAREFKGNRYDVGDKFGFMKTSIDYALEH
                     PEVKEDLKNYIIKLGKALEKSKVPTHSK"
BASE COUNT          336 a          235 c          238 g          402 t
ORIGIN      
        1 taaagttgta taacacaaaa cttttatatt ttggaacgtt gacaattttc aagatataaa
       61 cttaagttgc tataaacaag gaaaaaagta aagtaccaac acattacttt gaatgtgttg
      121 gtactttact tttttccaaa gcttttccta gtttgataat gtaatttttc aagtcctctt
      181 tgacctctgg gtgttctaag gcatagtcga tagatgtttt catgaatcca aatttatccc
      241 caacatcgta acgattacct ttaaattctc gtgcaaagac acgctgagtt ttattgaggg
      301 tatcgatagc atctgtgagt tgcacttcgt tacctgctcc aggggtctgt ctttccaaaa
      361 tatcaaaaat ttcaggggtt aggaggtaac gaccaataat agccaaatca ctaggcgcat
      421 cttctggttg tggtttttca acaaaggtgt ctacactgta aaggcccttg acagccttgc
      481 cttgaggagc gataacccca tagctagaca catcttcatg aggaactttc atcacagcga
      541 tagtggatgc atgcgtcttg tcatagtcct ccatgagttg tttggtgaga ggtttagcgg
      601 atgcatttgt aatgtccatt aagtcatctc caagcatgac cacaaagggt tcattgccca
      661 caaaggcttt ggcttgtaag acagcatctc ccagtcctct tgggtggctt tgacggataa
      721 aatgaaggtt aatggcagtg gtttcatcca ctaatttcaa cagttcattt ttccccttag
      781 cttggaggtt gtattctaat tcaaagtttg aatcaaaatg gtcctcgata gagcgtttag
      841 cttttccggt caccacaagg atttcctcga tgccagattt tagcgcttct tcgacgataa
      901 actggatggt tggtttatca acgatgggca acatctcttt ggcaagagct ttggtagcag
      961 gtaaaaaacg tgttcctaga cctgcagcag gaataatggc ttttctgact ttggtcatca
     1021 gtaactcctc actttttata atctatggct tagtgacatt cgttttcaga acggaattca
     1081 tttgacatca tgttacgttt tcatatgatt tttcgactat ttcatagtgc tcaatcatac
     1141 caccaactgc agttctttta aagtaaaaat gaatattatt gttatagatg aagacatggc
     1201 tagtctctat t
//
LOCUS       DATXIG010000133         1203 bp    DNA     linear   BCT 28-DEC-2023
DEFINITION  TPA_asm: Streptococcus pyogenes strain MGAS11113
            SAMN08555799-rid19682583.denovo.133, whole genome shotgun sequence.
ACCESSION   DATXIG010000133 DATXIG010000000
VERSION     DATXIG010000133.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN08555799
            Sequence Read Archive: SRR6740495
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pyogenes
  ORGANISM  Streptococcus pyogenes
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 1203)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1203)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-OCT-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-OCT-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001918058.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001918058.2
            Genome Coverage       :: 368x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/12/2023 11:23:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 1,806
            CDSs (total)                      :: 1,769
            Genes (coding)                    :: 1,722
            CDSs (with protein)               :: 1,722
            Genes (RNA)                       :: 37
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1, 1 (5S, 23S)
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 47
            CDSs (without protein)            :: 47
            Pseudo Genes (ambiguous residues) :: 0 of 47
            Pseudo Genes (frameshifted)       :: 30 of 47
            Pseudo Genes (incomplete)         :: 20 of 47
            Pseudo Genes (internal stop)      :: 9 of 47
            Pseudo Genes (multiple problems)  :: 11 of 47
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1203
                     /organism="Streptococcus pyogenes"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN08555799-rid19682583.denovo.133"
                     /strain="MGAS11113"
                     /serotype="Emm28"
                     /isolation_source="normally sterile site"
                     /host="Homo sapiens"
                     /db_xref="taxon:1314"
                     /country="Finland"
                     /collection_date="2002"
                     /collected_by="National Public Health Laboratory"
     gene            139..>1203
                     /locus_tag="VC122_001738"
     CDS             139..>1203
                     /locus_tag="VC122_001738"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011018339.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="insulinase family protein"
                     /protein_id="HEP5641633.1"
                     /translation="MKIVQGVQLHLIKTKQFKTNHITFRFSGDLNQKTVAKRVLVAQM
                     LATANECYPTVRQFREKLARLYGASLSTNVLTKGLVHIVDIDITFIQDRYACNGEKIL
                     DEMIQFLKDILFSPLLSIAQYQPKVFETEKNNLINYIESDREDSFYYSSLKVKELFYC
                     NKNLQMSEYGSPELIAKETAYTSYQEFHKMLNEDQIDIFILGDFDDYRVVQLIHQFPL
                     DNRNKNLNFFHLQNSVNIIKESIEKRAVHQSILQLAYHFPSVFGQRDYYALVLLNGLL
                     GSFAHSRLFIKIREEEGLAYSIGCRFDSYTGLFEIYTGIDSQHRTKTLQLIIQELNAI
                     KMGRFSEQLIKKTRSMLLNNA"
BASE COUNT          432 a          187 c          194 g          390 t
ORIGIN      
        1 ggtaaaggat atattgtaga aaactatatt ttactaacac tagaatcccg acaattaagt
       61 caaaaaaagt tatcactcac ctaacctgaa taaaaatggt ataatcgtaa ggatcacact
      121 atttattaag gaaacattat gaaaattgtt cagggagtac aacttcatct gattaagacc
      181 aaacagttta aaaccaatca tattaccttt cgtttttcag gtgatttgaa ccaaaaaaca
      241 gtagcaaaaa gagtcttagt ggcacagatg ttagcaacag ctaatgagtg ttatccaact
      301 gttaggcagt ttagagaaaa attagcacga ctttatggag ctagcttgtc aactaacgtg
      361 ttaacaaaag ggttagttca tattgtggat atcgatatta cttttattca agatagatat
      421 gcttgcaatg gggaaaaaat actagatgaa atgattcagt ttttaaaaga tatcttgttc
      481 tcacccctat tatccatcgc ccagtatcag cctaaagttt ttgagactga aaaaaataat
      541 ttgattaact acatcgagtc agatagagag gattcttttt actacagctc tcttaaggta
      601 aaagaattgt tttattgtaa taagaatcta caaatgtcag aatatggtag cccagaacta
      661 attgcaaaag agactgccta tactagctat caagaatttc ataaaatgct caatgaagat
      721 cagattgata ttttcatttt aggagatttt gatgattatc gcgttgttca gctaattcat
      781 caatttccac ttgataaccg aaataaaaac ctaaatttct ttcatttaca aaattctgtc
      841 aatattatta aagaatctat tgaaaaaaga gcagttcatc aatctatttt acaattagct
      901 tatcacttcc cttcagtttt tggacaacgt gactattatg ctttagttct tttaaatggg
      961 ttgttaggct cttttgcaca ttcccgtctc tttataaaaa ttagagaaga agaaggactt
     1021 gcttatagta tagggtgtcg ttttgatagt tatacaggtt tatttgaaat ttatactgga
     1081 attgacagtc aacatcgcac taaaacgtta caactaatca tccaagaatt aaatgccatt
     1141 aaaatgggac gattctcaga acaattgatt aaaaagacca gatcgatgtt gttgaataac
     1201 gcc
//
LOCUS       DATXIG010000134         1197 bp    DNA     linear   BCT 28-DEC-2023
DEFINITION  TPA_asm: Streptococcus pyogenes strain MGAS11113
            SAMN08555799-rid19682583.denovo.134, whole genome shotgun sequence.
ACCESSION   DATXIG010000134 DATXIG010000000
VERSION     DATXIG010000134.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN08555799
            Sequence Read Archive: SRR6740495
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pyogenes
  ORGANISM  Streptococcus pyogenes
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 1197)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1197)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-OCT-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-OCT-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001918058.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001918058.2
            Genome Coverage       :: 368x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/12/2023 11:23:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 1,806
            CDSs (total)                      :: 1,769
            Genes (coding)                    :: 1,722
            CDSs (with protein)               :: 1,722
            Genes (RNA)                       :: 37
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1, 1 (5S, 23S)
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 47
            CDSs (without protein)            :: 47
            Pseudo Genes (ambiguous residues) :: 0 of 47
            Pseudo Genes (frameshifted)       :: 30 of 47
            Pseudo Genes (incomplete)         :: 20 of 47
            Pseudo Genes (internal stop)      :: 9 of 47
            Pseudo Genes (multiple problems)  :: 11 of 47
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1197
                     /organism="Streptococcus pyogenes"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN08555799-rid19682583.denovo.134"
                     /strain="MGAS11113"
                     /serotype="Emm28"
                     /isolation_source="normally sterile site"
                     /host="Homo sapiens"
                     /db_xref="taxon:1314"
                     /country="Finland"
                     /collection_date="2002"
                     /collected_by="National Public Health Laboratory"
     gene            22..>1197
                     /locus_tag="VC122_001739"
     CDS             22..>1197
                     /locus_tag="VC122_001739"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000567306.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YSIRK-targeted surface antigen transcriptional
                     regulator"
                     /protein_id="HEP5641634.1"
                     /translation="MIDSLLLKSLHSLLGLTITVCDQNFSVIREYKSDKTISLFYNHY
                     LILSNFSKTQHDFLFHYGSLGELFLVHHIQQYYIIIGPWRSNAIDPLLLKKKLTETQI
                     NASEQDYFIDRLSQLPFFSLSQIRELLIVTNYCLTGVVKDKLSEPLHYYTKGWSNSFD
                     LDKIKQFSKQNMSSYKYQYHFENNILKAVKSGSEFLLKETVEHFSNSIVPIISGDELR
                     SEKNYSIIIYDRLSQATIQAGLDIETAYRARDRFIKETESTISLNEVLKLRDTAILFY
                     TQQVDSLKRHLETPHSQTIVAVIRYLENNLNRFIKTEEIAKECHMSESKLRKLFKQEK
                     HITIQQYFINLKIEAAKQLLDENKKVEEVSNLLGFSTSSNFSRTFKKIVGISPLEYKQ
                     "
BASE COUNT          435 a          205 c          167 g          390 t
ORIGIN      
        1 aaatcaaagg tggtaactca tatgattgat tctttattgt tgaaatcatt acacagtctt
       61 ctaggtctaa ctattactgt ttgtgatcaa aatttttctg ttatcagaga gtataaatcc
      121 gataaaacta tttcactttt ttacaatcat taccttattt taagtaactt tagtaaaact
      181 caacatgatt ttttatttca ttatggctct ttaggagaac tttttcttgt ccaccatatt
      241 caacaatatt atattatcat cggtccctgg cgtagcaatg ctattgatcc tttacttctt
      301 aagaaaaagc tgactgaaac acaaatcaat gctagtgaac aagactactt tattgataga
      361 ctatcccaat tacctttctt ctcacttagt caaatccgag aattactaat agtaaccaat
      421 tattgtctaa ctggggttgt gaaagataaa ttatcagagc ccttacatta ctacacgaaa
      481 gggtggagca actcctttga tctagataaa attaagcaat tttctaaaca gaatatgagt
      541 tcttacaaat atcagtatca ttttgaaaat aatattctaa aagctgttaa atcaggtagt
      601 gaatttcttt tgaaagaaac agtggaacac tttagcaatt ctatcgtacc tataatcagt
      661 ggagatgaat tgcgatctga aaaaaactac tccatcatta tttatgatcg tctctcacag
      721 gctaccatcc aagcaggact tgatatcgaa acagcttatc gagcacgaga tcgttttata
      781 aaagaaaccg agtcaactat aagtctaaat gaagttttaa aattacgtga tactgctatc
      841 ttattctata ctcaacaagt tgattcttta aaaagacatc tcgaaacccc tcattctcaa
      901 actattgtcg cagtgattcg atatcttgaa aataatctaa atcgctttat taaaacagaa
      961 gaaatagcta aagaatgtca catgagtgaa tcgaagttac gaaaactatt taagcaagaa
     1021 aaacacatca ctattcaaca atattttata aacttaaaaa tcgaagctgc taagcagtta
     1081 ctagacgaaa ataagaaagt agaagaagtt tccaatttac ttggattttc cacttcttct
     1141 aatttttcaa ggacatttaa aaaaatagtg ggaattagcc cactagaata taagcaa
//
LOCUS       DATXIG010000135         1194 bp    DNA     linear   BCT 28-DEC-2023
DEFINITION  TPA_asm: Streptococcus pyogenes strain MGAS11113
            SAMN08555799-rid19682583.denovo.135, whole genome shotgun sequence.
ACCESSION   DATXIG010000135 DATXIG010000000
VERSION     DATXIG010000135.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN08555799
            Sequence Read Archive: SRR6740495
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pyogenes
  ORGANISM  Streptococcus pyogenes
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 1194)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1194)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-OCT-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-OCT-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001918058.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001918058.2
            Genome Coverage       :: 368x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/12/2023 11:23:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 1,806
            CDSs (total)                      :: 1,769
            Genes (coding)                    :: 1,722
            CDSs (with protein)               :: 1,722
            Genes (RNA)                       :: 37
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1, 1 (5S, 23S)
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 47
            CDSs (without protein)            :: 47
            Pseudo Genes (ambiguous residues) :: 0 of 47
            Pseudo Genes (frameshifted)       :: 30 of 47
            Pseudo Genes (incomplete)         :: 20 of 47
            Pseudo Genes (internal stop)      :: 9 of 47
            Pseudo Genes (multiple problems)  :: 11 of 47
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1194
                     /organism="Streptococcus pyogenes"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN08555799-rid19682583.denovo.135"
                     /strain="MGAS11113"
                     /serotype="Emm28"
                     /isolation_source="normally sterile site"
                     /host="Homo sapiens"
                     /db_xref="taxon:1314"
                     /country="Finland"
                     /collection_date="2002"
                     /collected_by="National Public Health Laboratory"
     gene            56..>1194
                     /locus_tag="VC122_001740"
     CDS             56..>1194
                     /locus_tag="VC122_001740"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002995765.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="OFA family MFS transporter"
                     /protein_id="HEP5641635.1"
                     /translation="MEKTKRYIIATAGILLHLMLGSTYAWSVYRNPILQETGWDQAPV
                     AFAFSLAIFCLGLSAAFMGNLVEQYGPRLTGTVSAILYASGNMLTGLAIDRKEIWLLY
                     IGYGVIGGLGLGAGYITPISTIIKWFPDKRGMATGFAIMGFGFASLLTSPIAQWLIET
                     EGLVATFYLLGLIYLIVMLFASQLIIKPTAAEIAILDKKRLQNNSYLIEGMTAKEALK
                     TKSFYCLWVILFINITCGLGLISVVAPMAQDLTGMSPEMSAIVVGAMGIFNGFGRLVW
                     ASLSDYIGRRVTVILLFLVSIIMTISLIFAHSSLIFMISIATLMTCYGAGFSLIPPYL
                     SDLFGAKELATLHGYILTAWAIAALTGPMLLSITVEWTHNYLLTL"
BASE COUNT          332 a          185 c          244 g          433 t
ORIGIN      
        1 taacatttat gttttttaaa aaataaacgt tcgtataata aaaaaggagt tttttatgga
       61 aaaaacaaaa cgttatatca ttgcaacagc agggatactt ctccacttaa tgttgggatc
      121 gacctatgct tggagtgttt atcgtaatcc aattttacaa gagaccggtt gggatcaagc
      181 tcctgtcgct tttgcctttt ctttagctat tttttgttta ggtttatctg ctgcttttat
      241 gggaaattta gttgaacaat atggtcctcg tctaaccgga acggtgtcgg ctattcttta
      301 tgcttctgga aatatgctga ctggtttagc gattgatcgt aaagaaattt ggcttttata
      361 tattggttat ggtgttattg gtggcttagg acttggagcg ggatatatca caccgatatc
      421 aactattata aaatggtttc cagataaaag aggcatggca acagggtttg ctattatggg
      481 gtttggtttt gcgtctttat taacaagtcc gattgcccag tggctaattg aaacagaagg
      541 tttggtagca acattttacc ttttagggct gatttatcta atagttatgc tttttgcttc
      601 tcaattgata ataaaaccaa ctgctgctga gatagctata ctagataaaa aaagattaca
      661 aaataacagt tatttaatcg aaggaatgac cgctaaagaa gcacttaaaa caaaatcatt
      721 ttattgtctt tgggtaattt tatttatcaa tatcacatgt ggcttaggac ttatctcagt
      781 tgttgcgcca atggcacaag atctaactgg tatgagtcca gaaatgtcag caattgtggt
      841 tggtgctatg gggattttta acggtttcgg tcgtttagtg tgggccagtt tgtcagacta
      901 tattggacgc cgtgtgacag taatactttt atttttagta agcataatta tgactattag
      961 tttaatattt gcacatagtt ccctaatttt catgataagc attgccaccc ttatgacttg
     1021 ttatggtgcg ggtttttctc tcattccgcc ttatttaagt gatctttttg gggcaaagga
     1081 actggctaca ttacatgggt atatattgac agcttgggca atagctgcat taactggacc
     1141 tatgttatta tctatcactg ttgaatggac tcataattat cttcttacct tatg
//
LOCUS       DATXIG010000136         1167 bp    DNA     linear   BCT 28-DEC-2023
DEFINITION  TPA_asm: Streptococcus pyogenes strain MGAS11113
            SAMN08555799-rid19682583.denovo.136, whole genome shotgun sequence.
ACCESSION   DATXIG010000136 DATXIG010000000
VERSION     DATXIG010000136.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN08555799
            Sequence Read Archive: SRR6740495
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pyogenes
  ORGANISM  Streptococcus pyogenes
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 1167)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1167)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-OCT-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-OCT-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001918058.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001918058.2
            Genome Coverage       :: 368x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/12/2023 11:23:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 1,806
            CDSs (total)                      :: 1,769
            Genes (coding)                    :: 1,722
            CDSs (with protein)               :: 1,722
            Genes (RNA)                       :: 37
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1, 1 (5S, 23S)
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 47
            CDSs (without protein)            :: 47
            Pseudo Genes (ambiguous residues) :: 0 of 47
            Pseudo Genes (frameshifted)       :: 30 of 47
            Pseudo Genes (incomplete)         :: 20 of 47
            Pseudo Genes (internal stop)      :: 9 of 47
            Pseudo Genes (multiple problems)  :: 11 of 47
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1167
                     /organism="Streptococcus pyogenes"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN08555799-rid19682583.denovo.136"
                     /strain="MGAS11113"
                     /serotype="Emm28"
                     /isolation_source="normally sterile site"
                     /host="Homo sapiens"
                     /db_xref="taxon:1314"
                     /country="Finland"
                     /collection_date="2002"
                     /collected_by="National Public Health Laboratory"
     gene            complement(337..1110)
                     /gene="cfa"
                     /locus_tag="VC122_001741"
     CDS             complement(337..1110)
                     /gene="cfa"
                     /locus_tag="VC122_001741"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_149017463.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CAMP factor pore-forming toxin Cfa"
                     /protein_id="HEP5641636.1"
                     /translation="MKPKHLLCLSTVVAGLALFSTMTHSVLADDASNPDTILMNNNQA
                     NLQRDALVQKLDEGHQQLEAIKHEAKGTDIEATVNKAIDAVDHMKSSIRFNTETIYDF
                     SSIGARVEALSDAIKAIVFSTTQLTHKVEKAHTDMGFAITKLVIRIIDPFASVDAIKA
                     QVQEIKALEEKVINYPDLQPTDRATIYTKAKLNKAIWNTRLERDKKVLGIKSFDVYNR
                     LNKAITHAVGVQLNPTTTVQQVDDEVIAVQNALETALKS"
BASE COUNT          375 a          197 c          190 g          405 t
ORIGIN      
        1 catgatattc ctaaagcgat tcctagtccc aaaataataa gttaaatagt taaaaaaatc
       61 cctacaattt gtttttaaac agatcatagg gattttttta agaatacacg ttttcatatt
      121 gaatcttatt agtttaggag gttaaacaag attcaatagt gacggttgaa caatacctta
      181 aattggtaca tcatgttaaa ggcaaaaagg gctacaggtg actcattgtt tatgagaaca
      241 tcacctcaaa ctctacaatg gtaagatatt taaaatcttt acctattcta tatcctaata
      301 taatcatcta cctatacaac acattactta tacactctat gacttaagag ctgtttccaa
      361 agcattttgc acagcaataa cttcatcgtc aacttgttga acagtggttg tcggatttag
      421 ttggacacca accgcatgtg tgatagcttt gttaagtcta ttataaacgt caaatgattt
      481 gatccctaaa acctttttat ctctttctaa acgagtgttc caaatagctt tatttagttt
      541 agctttagta tagatagtag cacggtctgt tggctgtaaa tcaggataat tgataacttt
      601 ttcctcaagt gctttaattt cttgtacttg agctttgata gcgtcaacag atgcaaatgg
      661 atctataata cgaatgacta atttagtaat agcaaacccc atatcagtat gcgctttttc
      721 tactttgtga gttaattggg ttgtggaaaa tacgattgct ttgatagcat ctgataatgc
      781 ttctactctt gccccaattg aactaaaatc atagatcgtt tcagtattga agcgtataga
      841 actcttcatg tgatcaacag catcgatagc tttgttaaca gtagcctcaa tatcagtacc
      901 tttagcttca tgtttaatag cttctaattg ttgatgtccc tcatcaagct tttgcactag
      961 agcgtctctt tgaagattcg cttggttgtt attcattaaa atagtatcgg gatttgaagc
     1021 atcatcagct aaaactgagt gcgtcatagt tgaaaataaa gctaatccag caacaactgt
     1081 tgaaagacag agaaggtgtt tgggtttcat attattcctc ctgaatataa taaacctatt
     1141 taaatagtta agtgaagtta aattaat
//
LOCUS       DATXIG010000137         1129 bp    DNA     linear   BCT 28-DEC-2023
DEFINITION  TPA_asm: Streptococcus pyogenes strain MGAS11113
            SAMN08555799-rid19682583.denovo.137, whole genome shotgun sequence.
ACCESSION   DATXIG010000137 DATXIG010000000
VERSION     DATXIG010000137.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN08555799
            Sequence Read Archive: SRR6740495
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pyogenes
  ORGANISM  Streptococcus pyogenes
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 1129)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1129)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-OCT-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-OCT-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001918058.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001918058.2
            Genome Coverage       :: 368x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/12/2023 11:23:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 1,806
            CDSs (total)                      :: 1,769
            Genes (coding)                    :: 1,722
            CDSs (with protein)               :: 1,722
            Genes (RNA)                       :: 37
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1, 1 (5S, 23S)
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 47
            CDSs (without protein)            :: 47
            Pseudo Genes (ambiguous residues) :: 0 of 47
            Pseudo Genes (frameshifted)       :: 30 of 47
            Pseudo Genes (incomplete)         :: 20 of 47
            Pseudo Genes (internal stop)      :: 9 of 47
            Pseudo Genes (multiple problems)  :: 11 of 47
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1129
                     /organism="Streptococcus pyogenes"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN08555799-rid19682583.denovo.137"
                     /strain="MGAS11113"
                     /serotype="Emm28"
                     /isolation_source="normally sterile site"
                     /host="Homo sapiens"
                     /db_xref="taxon:1314"
                     /country="Finland"
                     /collection_date="2002"
                     /collected_by="National Public Health Laboratory"
     gene            complement(<1..1118)
                     /locus_tag="VC122_001742"
     CDS             complement(<1..1118)
                     /locus_tag="VC122_001742"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009880290.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HEP5641637.1"
                     /translation="MYSKVIYRIGDVMFDFANNTFLAGLNPASLSLVAVYQSLESVIG
                     VLFNLFGGVIADSFKRKKIIITTNILCGTACLVLSFLTKEQWLVYAIVLTNVILAFMS
                     AFSSPSYKAFTKEIVKKDSISQLNSLLETTSTVIKVTVPMVAIFLYKLLGIHGVLLLD
                     GLSFLIAALLISFILPVNDEVVIKEKVTIREIFNDLKIGFKYVYSHKSIFIITVLSAL
                     VNFFLAAYNLLLPYSNQMFGEISTGLYGTFLTAEAIGGFIGAILSGFVNKELSSMRLI
                     LFLSLSGLMLMLAPPFYIMFHNAIILALSPALFSLFLSIFNIQFFSLVQKDVDNDFLG
                     RVFGIIFTITILFMPIGTGFFSVALNPNNSFNLFIIGS"
BASE COUNT          452 a          188 c          162 g          327 t
ORIGIN      
        1 cacgaaccaa taataaaaag attaaagcta ttgttaggat ttaatgccac cgagaaaaag
       61 ccagtcccaa taggcataaa aagaatagtt attgtaaaga taataccaaa cactctccct
      121 aaaaaatcgt tatcaacatc tttctggaca agagaaaaaa attgaatatt aaagatggaa
      181 agaaataaac tgaataaagc tggtgacaaa gctaaaataa ttgcattatg aaacataata
      241 taaaatgggg gagctaacat taacatcagt cctgataaag atagaaacaa tatcaatctc
      301 atacttgata attctttatt cacaaaacca cttaatattg ccccaataaa tccaccaatt
      361 gcttctgctg ttaaaaaagt gccataaagt ccagttgaaa tttctccaaa catttgatta
      421 ctatagggca acaataaatt ataagcggct agaaaaaaat taacaagtgc agaaagaacg
      481 gtaataataa agatggactt atggctgtaa acatatttaa atcctatttt taaatcgtta
      541 aatatttctc tgattgtcac cttctccttt atcaccactt cgtcattaac aggtaaaata
      601 aaggaaatta gtaaagcagc tattagaaat gatagtccat ccaatagtaa tacaccgtgt
      661 ataccaagta gcttatataa gaaaattgct accataggaa ctgttacttt gattacagta
      721 cttgttgtct ctagcaatga attaagttgt gatatactat cttttttaac aatttctttt
      781 gtaaatgctt tataggatgg actagaaaaa gcactcataa atgccaagat aacattagtt
      841 agtacaatgg cataaaccaa ccattgctct tttgttagga acgagagtac taagcaagct
      901 gtgccacata agatatttgt agtaataata atttttttcc gcttgaaact gtctgcaatg
      961 actccaccaa ataaattaaa tagaacacct atgacacttt ctagagactg atatactgca
     1021 actaatgata gagacgctgg attaagccca gcaagaaaag tattgttagc aaaatcaaac
     1081 ataacatcgc caattcgata tataacttta ctatacacta aatattgaa
//
LOCUS       DATXIG010000138         1054 bp    DNA     linear   BCT 28-DEC-2023
DEFINITION  TPA_asm: Streptococcus pyogenes strain MGAS11113
            SAMN08555799-rid19682583.denovo.138, whole genome shotgun sequence.
ACCESSION   DATXIG010000138 DATXIG010000000
VERSION     DATXIG010000138.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN08555799
            Sequence Read Archive: SRR6740495
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pyogenes
  ORGANISM  Streptococcus pyogenes
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 1054)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1054)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-OCT-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-OCT-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001918058.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001918058.2
            Genome Coverage       :: 368x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/12/2023 11:23:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 1,806
            CDSs (total)                      :: 1,769
            Genes (coding)                    :: 1,722
            CDSs (with protein)               :: 1,722
            Genes (RNA)                       :: 37
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1, 1 (5S, 23S)
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 47
            CDSs (without protein)            :: 47
            Pseudo Genes (ambiguous residues) :: 0 of 47
            Pseudo Genes (frameshifted)       :: 30 of 47
            Pseudo Genes (incomplete)         :: 20 of 47
            Pseudo Genes (internal stop)      :: 9 of 47
            Pseudo Genes (multiple problems)  :: 11 of 47
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1054
                     /organism="Streptococcus pyogenes"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN08555799-rid19682583.denovo.138"
                     /strain="MGAS11113"
                     /serotype="Emm28"
                     /isolation_source="normally sterile site"
                     /host="Homo sapiens"
                     /db_xref="taxon:1314"
                     /country="Finland"
                     /collection_date="2002"
                     /collected_by="National Public Health Laboratory"
     gene            complement(34..>1054)
                     /locus_tag="VC122_001743"
     CDS             complement(34..>1054)
                     /locus_tag="VC122_001743"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019418808.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEP5641638.1"
                     /translation="LEIAIQSIIMFLAVREAFLGILLVGNVTTFIRSLSTMQTSTQNI
                     VNNIYSFYNGSLYMELLYDFTHDELVHNPIIESTVDTIQSVEFERVSFSYNGKDNVIN
                     DISFSIKAGERLAIVGENGSGKSTIFKLACGLYDNYEGNIYINGINLRSIQKKSYYKR
                     ISALFQDFLKYELTLRENVGLGELSKLYSDEDLISALKYTGVDSIFYFNQEKLNQIDL
                     EQQLGNWFEDGRQLSGGQWQKIALSRVYLKEADCYLLDEPSSALDPESETKIFKTFFE
                     LSQNKIGIFITHKPSITQSVNKILYLDNGCIAETGSFADLNIKGMKFKNLLDKEIGAT
                     YDTSN"
BASE COUNT          353 a          199 c          120 g          382 t
ORIGIN      
        1 ataaaactat ctgcaatgta atctactatc tgtttaattt gaggtgtcat atgtggctcc
       61 aatttcttta tctaataagt ttttaaactt cattccttta atatttaaat cagcgaaact
      121 tcctgtttca gctatacatc cattatctag atacaaaatt ttattcacag attgagtaat
      181 gcttggctta tgcgtaataa aaataccaat tttattttgc gataattcaa aaaatgtttt
      241 aaaaattttt gtttcagact caggatctaa cgcagaacta ggctcatcta gcaaatagca
      301 atccgcttct ttgagatata ctcttgataa tgcaattttt tgccactgtc caccagataa
      361 ctgtctccca tcctcaaacc aattccctaa ttgctgttcc agatctatct gatttaattt
      421 ttcctgatta aagtaaaata tcgaatcaac tcctgtatac ttcagtgcac ttattaaatc
      481 ctcatcacta tataactttg ataattcacc caatccaaca ttttcacgca gtgtcaattc
      541 atactttaaa aaatcttgga acaatgcgga tattctcttg tagtatgact ttttctgtat
      601 tgaacgaaga ttaataccat ttatatatat attgccttca taattatcgt aaagaccgca
      661 ggctaatttg aaaatcgtac tttttccaga accattttca ccaacaattg ctaacctctc
      721 tcctgcttta attgaaaaac ttatatcgtt tattacatta tccttaccat tatatgaaaa
      781 agaaactctt tcaaattcaa ctgactgaat tgtatcaacc gtactttcga ttatagggtt
      841 atgtactaac tcatcatgcg taaaatcata caataactcc atgtacaatg aaccattata
      901 gaaagaatag atattattta ctatattttg tgtacttgtt tgcattgtag ataaagacct
      961 tataaaagta gttacatttc caactaataa tattcctaga aatgcttctc taactgcaag
     1021 gaacataata atagattgta tagcaatctc caat
//
LOCUS       DATXIG010000139         1010 bp    DNA     linear   BCT 28-DEC-2023
DEFINITION  TPA_asm: Streptococcus pyogenes strain MGAS11113
            SAMN08555799-rid19682583.denovo.139, whole genome shotgun sequence.
ACCESSION   DATXIG010000139 DATXIG010000000
VERSION     DATXIG010000139.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN08555799
            Sequence Read Archive: SRR6740495
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pyogenes
  ORGANISM  Streptococcus pyogenes
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 1010)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1010)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-OCT-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-OCT-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001918058.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001918058.2
            Genome Coverage       :: 368x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/12/2023 11:23:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 1,806
            CDSs (total)                      :: 1,769
            Genes (coding)                    :: 1,722
            CDSs (with protein)               :: 1,722
            Genes (RNA)                       :: 37
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1, 1 (5S, 23S)
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 47
            CDSs (without protein)            :: 47
            Pseudo Genes (ambiguous residues) :: 0 of 47
            Pseudo Genes (frameshifted)       :: 30 of 47
            Pseudo Genes (incomplete)         :: 20 of 47
            Pseudo Genes (internal stop)      :: 9 of 47
            Pseudo Genes (multiple problems)  :: 11 of 47
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1010
                     /organism="Streptococcus pyogenes"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN08555799-rid19682583.denovo.139"
                     /strain="MGAS11113"
                     /serotype="Emm28"
                     /isolation_source="normally sterile site"
                     /host="Homo sapiens"
                     /db_xref="taxon:1314"
                     /country="Finland"
                     /collection_date="2002"
                     /collected_by="National Public Health Laboratory"
     gene            158..439
                     /locus_tag="VC122_001744"
     CDS             158..439
                     /locus_tag="VC122_001744"
                     /inference="COORDINATES: protein motif:HMM:NF013753.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HEP5641639.1"
                     /translation="MTVLNYVNAGDAKSIHNHLFHYTRQVRRHVKVITIGILSAHYYE
                     ITMLFLQAKIILDCFHIPQRLRQAITRFRIRIINTSAIKPIAYRTLKLY"
     gene            complement(916..1003)
                     /locus_tag="VC122_001745"
     tRNA            complement(916..1003)
                     /locus_tag="VC122_001745"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(967..969),aa:Ser,seq:gct)
BASE COUNT          344 a          216 c          117 g          333 t
ORIGIN      
        1 gtttatcttc actcagacac cttatccttc ttagaatgaa tttttagtta cttatccaca
       61 ggttaataac ttcttttcgc ctaaaatatt atccacaata gtgacatttt ctattatcag
      121 ttatagtata atttcattag gaatctaaaa ccttagcatc acggttttaa attatgtaaa
      181 tgctggggac gcaaaaagca ttcataatca tctctttcat tataccagac aggtgagaag
      241 gcatgtcaaa gttattacca taggcatact cagtgcccac tactatgaga ttactatgct
      301 ttttctacag gctaaaatta tcctcgattg ttttcatatc ccccaacgtc ttagacaagc
      361 tataactcgc tttcgtatcc gaatcataaa tacttctgct ataaaaccta tcgcgtatag
      421 aactctcaaa ctttactgaa aactcataca gcatgattca cggaagctca tcaataatag
      481 ctttattgcc caatttttcg tacgcccata actaacatct agaaaagaaa agctcttact
      541 cacaagatct ctgaatttca ataattattc ctaactctat caactcttgc tcttcccatt
      601 tttcagacaa tttttaaaat ttttctaaaa gaaaagaaaa caagattagc aatatactta
      661 aactcaacta ctctaaggcc aaattagaaa cagtcaacaa ccttatcaaa gccatcaagt
      721 taaacgtttt actcctctac agtagtcaaa gagcctatca ttttagttat cgaaataatg
      781 aacgatttaa atattctatt tagttggttt tgaagattac aaaaaaaaga cgataaccgt
      841 cttctttccc ccttatttat aattcatcct ttaatatgtt tcatttaaca tattaaccaa
      901 aatatcttta caaaacggag aataagggat tcgaaccctt gcgccagtta cccgacctaa
      961 cgatttagca aaccgtcctc ttcagcctct tgagtaattc tccaaatata
//
LOCUS       DATXIG010000140         1004 bp    DNA     linear   BCT 28-DEC-2023
DEFINITION  TPA_asm: Streptococcus pyogenes strain MGAS11113
            SAMN08555799-rid19682583.denovo.140, whole genome shotgun sequence.
ACCESSION   DATXIG010000140 DATXIG010000000
VERSION     DATXIG010000140.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN08555799
            Sequence Read Archive: SRR6740495
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pyogenes
  ORGANISM  Streptococcus pyogenes
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 1004)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1004)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-OCT-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-OCT-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001918058.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001918058.2
            Genome Coverage       :: 368x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/12/2023 11:23:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 1,806
            CDSs (total)                      :: 1,769
            Genes (coding)                    :: 1,722
            CDSs (with protein)               :: 1,722
            Genes (RNA)                       :: 37
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1, 1 (5S, 23S)
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 47
            CDSs (without protein)            :: 47
            Pseudo Genes (ambiguous residues) :: 0 of 47
            Pseudo Genes (frameshifted)       :: 30 of 47
            Pseudo Genes (incomplete)         :: 20 of 47
            Pseudo Genes (internal stop)      :: 9 of 47
            Pseudo Genes (multiple problems)  :: 11 of 47
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1004
                     /organism="Streptococcus pyogenes"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN08555799-rid19682583.denovo.140"
                     /strain="MGAS11113"
                     /serotype="Emm28"
                     /isolation_source="normally sterile site"
                     /host="Homo sapiens"
                     /db_xref="taxon:1314"
                     /country="Finland"
                     /collection_date="2002"
                     /collected_by="National Public Health Laboratory"
     gene            <1..211
                     /locus_tag="VC122_001746"
     CDS             <1..211
                     /locus_tag="VC122_001746"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001059491.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEP5641640.1"
                     /translation="TKYENKSDNIKSQYLEVIDWAKANLKRRSWIDTIQYYDLEDKGF
                     NIKIIGKLSDRDIERLKDFLRAKEV"
     gene            214..570
                     /locus_tag="VC122_001747"
     CDS             214..570
                     /locus_tag="VC122_001747"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000728322.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEP5641641.1"
                     /translation="MKKKLVGFIVLALSTIILVACSNDSLEGEYYWINDARNQHMATI
                     KGDKGYVESEGGYSIKIDSELKIIESKFGSEKYSYKDGKLTTNFTGVESDFYKKGSKA
                     CEEALKKYGYKEVGKE"
     gene            complement(635..>1004)
                     /locus_tag="VC122_001748"
     CDS             complement(635..>1004)
                     /locus_tag="VC122_001748"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000366192.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="ImmA/IrrE family metallo-endopeptidase"
                     /protein_id="HEP5641642.1"
                     /translation="FCNMPKTKGRYDSTLEKPCIYVDKSLSDIEKINIILHEKMHFTK
                     NDQSNCLSYISSYSHHIENQAEKDRILDFMNLINEEYPIDESFNYINYMKNACVPEKY
                     ESYIKNLAKEFYLRNHRKES"
BASE COUNT          366 a           98 c          179 g          361 t
ORIGIN      
        1 tactaaatat gaaaataagt ctgacaatat aaaaagtcag tatttagaag ttattgattg
       61 ggcaaaagca aatttaaaaa ggcgttcatg gattgataca attcagtact atgatttaga
      121 ggacaaggga ttcaatatta aaattattgg taagttaagt gatagagata ttgaaagatt
      181 aaaagatttt ctcagagcaa aggaagttta gttatgaaaa agaaattagt tggttttatt
      241 gttctagctt tatctacaat aattttggta gcatgttcta atgattcact tgaaggtgaa
      301 tattattgga ttaatgatgc tagaaatcag catatggcta caattaaagg cgataagggt
      361 tatgttgaat cagaaggcgg ttattccata aaaattgatt cagagttaaa aattattgaa
      421 agtaaatttg gttctgaaaa atatagttat aaggatggaa aattgacaac aaattttact
      481 ggtgtagaaa gtgattttta taaaaaggga tcaaaagctt gtgaggaagc attgaaaaaa
      541 tatggttata aagaagtagg aaaagaatag tattttatta tattctgttc catgaaaatt
      601 atttttcaga ataaaaagga gagctaacta actactagct ctcttttctg tgatttctta
      661 gataaaattc tttagcgaga tttttgatat aactttcata cttttcaggt acacaagcat
      721 ttttcatata gttgatatag ttaaaagatt catcaatcgg atattcttcg ttaattagat
      781 tcataaaatc taatatgcgg tctttttcag cttgattttc gatatgatgt gaatatgaag
      841 aaatataaga taagcagtta ctctgatcat tttttgtaaa gtgcattttt tcatgaagaa
      901 tgatgttaat tttttctata tctgaaagac ttttgtcaac ataaatgcaa ggcttttcaa
      961 gtgtgctgtc atatcttcct ttggtttttg gcatattaca aaat
//
LOCUS       DATXIG010000141          969 bp    DNA     linear   BCT 28-DEC-2023
DEFINITION  TPA_asm: Streptococcus pyogenes strain MGAS11113
            SAMN08555799-rid19682583.denovo.141, whole genome shotgun sequence.
ACCESSION   DATXIG010000141 DATXIG010000000
VERSION     DATXIG010000141.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN08555799
            Sequence Read Archive: SRR6740495
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pyogenes
  ORGANISM  Streptococcus pyogenes
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 969)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 969)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-OCT-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-OCT-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001918058.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001918058.2
            Genome Coverage       :: 368x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/12/2023 11:23:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 1,806
            CDSs (total)                      :: 1,769
            Genes (coding)                    :: 1,722
            CDSs (with protein)               :: 1,722
            Genes (RNA)                       :: 37
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1, 1 (5S, 23S)
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 47
            CDSs (without protein)            :: 47
            Pseudo Genes (ambiguous residues) :: 0 of 47
            Pseudo Genes (frameshifted)       :: 30 of 47
            Pseudo Genes (incomplete)         :: 20 of 47
            Pseudo Genes (internal stop)      :: 9 of 47
            Pseudo Genes (multiple problems)  :: 11 of 47
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..969
                     /organism="Streptococcus pyogenes"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN08555799-rid19682583.denovo.141"
                     /strain="MGAS11113"
                     /serotype="Emm28"
                     /isolation_source="normally sterile site"
                     /host="Homo sapiens"
                     /db_xref="taxon:1314"
                     /country="Finland"
                     /collection_date="2002"
                     /collected_by="National Public Health Laboratory"
     gene            <1..819
                     /locus_tag="VC122_001749"
     CDS             <1..819
                     /locus_tag="VC122_001749"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011528380.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine/threonine protein kinase"
                     /protein_id="HEP5641643.1"
                     /translation="INSNQQLIEYDGNGNILVYGRLKYFKEGISLYYIGEYLAECLLI
                     EHSDTLLIHAAAVYNPLSGHSILLLGDKGAGKTTVAIRLCIEHGYHLIGNDQVIFGSN
                     SGILLTYAGTSFFKIRRTAVLSDNLLFKLFSKFFNRSLQFNKASWDDKISIFPKELGI
                     RTCNFSTEISKIYYIKTDKKEKQIYHSIWSDSLASLYLNELLGRHISGQVACFQSDNG
                     KYFSTMPLINYEKNNRVREQIVKGIFAKQKLFKITAATPAKIAEAIIENVKKNS"
BASE COUNT          357 a          114 c          185 g          313 t
ORIGIN      
        1 attaacagca atcagcaact tatagaatat gatggcaatg gaaatatttt agtttatgga
       61 agattaaagt attttaaaga aggaatttca ttatactaca ttggagagta cttagctgaa
      121 tgtctcttga ttgaacatag tgacacctta ttaattcatg cagcagcagt ttataatcct
      181 ttatctggcc attcaatttt actattagga gataaaggtg ctggaaaaac tacggttgca
      241 ataaggctgt gtatagaaca tgggtatcac cttattggta atgaccaagt aatatttggt
      301 tcgaactctg gaatactact aacttatgca gggacatctt tctttaaaat aagaaggaca
      361 gctgttttgt cggacaattt actgtttaaa ttatttagca aattttttaa ccgttcatta
      421 caatttaata aggcaagttg ggatgataaa atttcaattt tcccaaagga gttgggaata
      481 cgaacttgta atttttctac tgaaatctca aaaatttatt atataaagac agacaagaaa
      541 gaaaagcaaa tttatcattc aatttggagt gatagtttag caagtctata tctaaatgag
      601 ctattgggaa ggcatatttc tggacaggtt gcatgttttc aatcagataa tggaaaatat
      661 ttttctacaa tgcctttgat aaattatgaa aaaaataatc gggtacgaga acagatagtg
      721 aagggtattt tcgctaaaca aaaattattt aaaattacag cagctacgcc agctaagatt
      781 gcagaagcga ttatagaaaa tgttaagaaa aactcatagt gtgtgtgaat ttggggatta
      841 atatgaagta ttgaaaaagg agtaagagta attatgtttg tgataggcat tccaacttta
      901 aatgaggcag ataatatcag tagattagtt aaacaaattg atgagtatgc tgtaaattta
      961 gggaaagaa
//
LOCUS       DATXIG010000142          965 bp    DNA     linear   BCT 28-DEC-2023
DEFINITION  TPA_asm: Streptococcus pyogenes strain MGAS11113
            SAMN08555799-rid19682583.denovo.142, whole genome shotgun sequence.
ACCESSION   DATXIG010000142 DATXIG010000000
VERSION     DATXIG010000142.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN08555799
            Sequence Read Archive: SRR6740495
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pyogenes
  ORGANISM  Streptococcus pyogenes
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 965)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 965)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-OCT-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-OCT-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001918058.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001918058.2
            Genome Coverage       :: 368x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/12/2023 11:23:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 1,806
            CDSs (total)                      :: 1,769
            Genes (coding)                    :: 1,722
            CDSs (with protein)               :: 1,722
            Genes (RNA)                       :: 37
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1, 1 (5S, 23S)
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 47
            CDSs (without protein)            :: 47
            Pseudo Genes (ambiguous residues) :: 0 of 47
            Pseudo Genes (frameshifted)       :: 30 of 47
            Pseudo Genes (incomplete)         :: 20 of 47
            Pseudo Genes (internal stop)      :: 9 of 47
            Pseudo Genes (multiple problems)  :: 11 of 47
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..965
                     /organism="Streptococcus pyogenes"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN08555799-rid19682583.denovo.142"
                     /strain="MGAS11113"
                     /serotype="Emm28"
                     /isolation_source="normally sterile site"
                     /host="Homo sapiens"
                     /db_xref="taxon:1314"
                     /country="Finland"
                     /collection_date="2002"
                     /collected_by="National Public Health Laboratory"
     gene            complement(<1..928)
                     /locus_tag="VC122_001750"
     CDS             complement(<1..928)
                     /locus_tag="VC122_001750"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003050939.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing histidine kinase"
                     /protein_id="HEP5641644.1"
                     /translation="MTIIILQIMRFGVYSSVDSSLVSVSNNASSYANRTMARISSFYF
                     DTENNIIKALPDSDSSKLLGTPAANTDIILFSANGTILNAFDAFSNYQNFHLDKRRLG
                     SIETTSLMNFYGQEEKYHTITVRVHIKNYPAVAYMMAVVNVEQLDRANERYERIIIIV
                     MSVFWLISILASIYLAKWSRKPILESYEKQKMFVENASHELRTPLAVLQNRLESLFRK
                     PNETILENSEHLASSLDEVRNMRILTTNLLNLARRDDGINPQWTHLDTDFFNAIFENY
                     ELVAKEYGKIFYFQNQVNRSLRMDKALLKQLIT"
BASE COUNT          335 a          178 c          138 g          314 t
ORIGIN      
        1 tcgttattaa ttgttttagt aaagccttat ccattcttaa cgatctattg acttggttct
       61 gaaaataaaa tatttttcca tattctttag caactagttc ataattctca aaaatagcat
      121 taaaaaaatc tgtatctaaa tgagtccact gtggattaat gccatcatct cgtcttgcta
      181 aatttaataa attagttgtt aagatgcgca tgttgcgaac ctcgtctaaa ctagaagcga
      241 gatgctcact attttctaat atcgtttcgt tgggcttacg aaaaagcgat tccagacgat
      301 tctgtaagac cgccaaaggg gtccttagtt catgactagc attttcaaca aacatttttt
      361 gtttttcata gctttctaaa ataggttttc tgctccactt ggctaaataa atacttgcta
      421 aaatagaaat tagccaaaaa acactcataa ctataataat aatgcgctcg taacgttcat
      481 tagcgcggtc taattgttcc acatttacta ctgccatcat ataggcaact gcaggataat
      541 ttttgatatg aacccttaca gttatcgtat ggtatttttc ttcttgtcca taaaaattca
      601 ttaaactggt ggtttcaata ctccccaacc ggcgtttatc taaatgaaaa ttttgatagt
      661 tagaaaacgc atcaaaagca tttaaaattg ttccattagc actaaacaaa atgatatctg
      721 tattagctgc aggcgttcct aataacttag aactatctga atcaggcagc gccttaataa
      781 tgttattttc agtatcaaag taaaaagaag atattctagc catcgtacga ttagcatagc
      841 tacttgcatt attactaaca gaaactaaac tactatcaac tgacgaataa acgccaaacc
      901 gcataatctg taagataata atagtcataa tgacaaaaat acctgtaaaa accgcaaaaa
      961 aatga
//
LOCUS       DATXIG010000143          925 bp    DNA     linear   BCT 28-DEC-2023
DEFINITION  TPA_asm: Streptococcus pyogenes strain MGAS11113
            SAMN08555799-rid19682583.denovo.143, whole genome shotgun sequence.
ACCESSION   DATXIG010000143 DATXIG010000000
VERSION     DATXIG010000143.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN08555799
            Sequence Read Archive: SRR6740495
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pyogenes
  ORGANISM  Streptococcus pyogenes
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 925)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 925)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-OCT-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-OCT-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001918058.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001918058.2
            Genome Coverage       :: 368x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/12/2023 11:23:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 1,806
            CDSs (total)                      :: 1,769
            Genes (coding)                    :: 1,722
            CDSs (with protein)               :: 1,722
            Genes (RNA)                       :: 37
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1, 1 (5S, 23S)
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 47
            CDSs (without protein)            :: 47
            Pseudo Genes (ambiguous residues) :: 0 of 47
            Pseudo Genes (frameshifted)       :: 30 of 47
            Pseudo Genes (incomplete)         :: 20 of 47
            Pseudo Genes (internal stop)      :: 9 of 47
            Pseudo Genes (multiple problems)  :: 11 of 47
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..925
                     /organism="Streptococcus pyogenes"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN08555799-rid19682583.denovo.143"
                     /strain="MGAS11113"
                     /serotype="Emm28"
                     /isolation_source="normally sterile site"
                     /host="Homo sapiens"
                     /db_xref="taxon:1314"
                     /country="Finland"
                     /collection_date="2002"
                     /collected_by="National Public Health Laboratory"
     gene            <1..674
                     /locus_tag="VC122_001751"
     CDS             <1..674
                     /locus_tag="VC122_001751"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018373772.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HEP5641645.1"
                     /translation="AIDDDKEILKLMKTALEIENYHVITCQEIELPIVFDDFKGYDLI
                     LLDIMMPNISGTEFCYKIREEVHSPIIFVSALDGDNEIVQALNIGGDDFIVKPFSLKQ
                     FVAKVNSHLKREERAKIKNEAEERVKRSFPPIEIYLEERMLYIDKQPLFLTYREYEIL
                     ELLSRHPYKVFTKEEIYEQVYSDEASALFHSISEYIYQIRMKFSSFGINPIKTIRGIG
                     YKWDV"
BASE COUNT          331 a          107 c          182 g          305 t
ORIGIN      
        1 tagccataga tgatgataaa gaaattctga agcttatgaa gactgctttg gagattgaaa
       61 attaccatgt tataacctgt caagagatag aattaccaat agtttttgat gattttaaag
      121 gatatgattt gatcttattg gatatcatga tgcctaatat aagtggaact gagttttgtt
      181 ataaaattcg ggaagaagtt cattctccaa ttatttttgt tagcgcttta gatggcgata
      241 atgaaattgt ccaagcttta aatatagggg gagacgattt tattgtgaaa ccatttagct
      301 taaaacaatt cgtagctaaa gttaactctc atttgaagag agaagagaga gcaaagataa
      361 agaatgaggc tgaggagaga gtgaagcgca gttttccacc tatagaaatc tatctagagg
      421 aacgtatgtt atatattgat aaacagccgt tattcctgac ttatagagag tacgaaattt
      481 tagaattact gtcacgtcat ccgtataaag ttttcacaaa agaagagata tatgaacaag
      541 tatatagcga tgaagcttca gcattgtttc attctatttc agaatatatc tatcagatta
      601 gaatgaaatt ttcaagtttt ggaattaatc caataaaaac tattcgcggg attggttata
      661 aatgggatgt ttaaaaaata ttcaataaaa aatcgtgttt ggcgcgccgt cgttgaaatt
      721 gtctttggag tttgtatcac tgttttaatg attgcgataa tatcgctttc tttttcaaag
      781 ttaaatatag tgacatctca taatgtggga gaagaatttt atattaaaga taaacagtca
      841 atcaaacagt tgaacaatta tatgaagaca ttgggattag attatgttgt ttttgataga
      901 aaaacagata aagctatgga aggaa
//
LOCUS       DATXIG010000144          900 bp    DNA     linear   BCT 28-DEC-2023
DEFINITION  TPA_asm: Streptococcus pyogenes strain MGAS11113
            SAMN08555799-rid19682583.denovo.144, whole genome shotgun sequence.
ACCESSION   DATXIG010000144 DATXIG010000000
VERSION     DATXIG010000144.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN08555799
            Sequence Read Archive: SRR6740495
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pyogenes
  ORGANISM  Streptococcus pyogenes
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 900)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 900)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-OCT-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-OCT-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001918058.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001918058.2
            Genome Coverage       :: 368x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/12/2023 11:23:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 1,806
            CDSs (total)                      :: 1,769
            Genes (coding)                    :: 1,722
            CDSs (with protein)               :: 1,722
            Genes (RNA)                       :: 37
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1, 1 (5S, 23S)
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 47
            CDSs (without protein)            :: 47
            Pseudo Genes (ambiguous residues) :: 0 of 47
            Pseudo Genes (frameshifted)       :: 30 of 47
            Pseudo Genes (incomplete)         :: 20 of 47
            Pseudo Genes (internal stop)      :: 9 of 47
            Pseudo Genes (multiple problems)  :: 11 of 47
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..900
                     /organism="Streptococcus pyogenes"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN08555799-rid19682583.denovo.144"
                     /strain="MGAS11113"
                     /serotype="Emm28"
                     /isolation_source="normally sterile site"
                     /host="Homo sapiens"
                     /db_xref="taxon:1314"
                     /country="Finland"
                     /collection_date="2002"
                     /collected_by="National Public Health Laboratory"
     gene            complement(19..92)
                     /locus_tag="VC122_001752"
     tRNA            complement(19..92)
                     /locus_tag="VC122_001752"
                     /product="tRNA-Ile"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(56..58),aa:Ile,seq:cat)
     gene            complement(113..186)
                     /locus_tag="VC122_001753"
     tRNA            complement(113..186)
                     /locus_tag="VC122_001753"
                     /product="tRNA-Met"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(150..152),aa:Met,seq:cat)
     gene            complement(192..265)
                     /locus_tag="VC122_001754"
     tRNA            complement(192..265)
                     /locus_tag="VC122_001754"
                     /product="tRNA-Pro"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(229..231),aa:Pro,seq:tgg)
     gene            complement(272..345)
                     /locus_tag="VC122_001755"
     tRNA            complement(272..345)
                     /locus_tag="VC122_001755"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(309..311),aa:Arg,seq:acg)
     gene            complement(362..446)
                     /locus_tag="VC122_001756"
     tRNA            complement(362..446)
                     /locus_tag="VC122_001756"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(410..412),aa:Leu,seq:taa)
     gene            complement(455..526)
                     /locus_tag="VC122_001757"
     tRNA            complement(455..526)
                     /locus_tag="VC122_001757"
                     /product="tRNA-Gly"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(492..494),aa:Gly,seq:gcc)
     gene            complement(539..611)
                     /locus_tag="VC122_001758"
     tRNA            complement(539..611)
                     /locus_tag="VC122_001758"
                     /product="tRNA-Thr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(576..578),aa:Thr,seq:tgt)
     gene            complement(622..703)
                     /locus_tag="VC122_001759"
     tRNA            complement(622..703)
                     /locus_tag="VC122_001759"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(667..669),aa:Leu,seq:tag)
     gene            complement(709..781)
                     /locus_tag="VC122_001760"
     tRNA            complement(709..781)
                     /locus_tag="VC122_001760"
                     /product="tRNA-Lys"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(746..748),aa:Lys,seq:ttt)
     gene            complement(813..885)
                     /locus_tag="VC122_001761"
     tRNA            complement(813..885)
                     /locus_tag="VC122_001761"
                     /product="tRNA-Asp"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(850..852),aa:Asp,seq:gtc)
BASE COUNT          241 a          261 c          197 g          201 t
ORIGIN      
        1 taatgattat ctatgatatg gaccttgtag gactcgaacc tacgaccgct cggttatgag
       61 ccgagtgctc taaccagttg agctaaaggt cccaagtcat ccataaaaat aatagcggcg
      121 aaggggatcg aacccccgac ctcccgggta tgaaccggac gctctagcca gctgagctac
      181 accgccatta atcgggaaga caggattcga acctgcgaca ccttggtccc aaaccaagta
      241 ctctaccaag ctgagctact tcccgaactg atgcacccta gaggagtcga acctctaacc
      301 gcctgattcg tagtcaggta ctctatccag ttgagctaag ggtgctattt caaaaaatta
      361 atgccgagga ccggaatcga accggtacga tgtaaaccat cgcaggattt taagtcctgt
      421 gcgtctgcca gttccgccac cccggctcct caaaagcgaa cgacgggatt cgaacccgcg
      481 acccccacct tggcaaggtg atgttctacc actgaactac gttcgcaact aatctcaatg
      541 ccggctacat gacttgaaca cgcgaccctc tgattacaaa tcagatgctc taccaactga
      601 gctaagccgg ctaatctttt atgcgggtta agggacttga acccccacgc cgttaagcgc
      661 cagatcctaa atctggtgcg tctgccaatt ccgccaaacc cgcttttatg acccgtactg
      721 ggctcgaacc agtgacccat tgattaaaag tcaattgctc taccaactga gctaacgagt
      781 ctttccttca aaaaatatga ctactctttg aacggtcccg acgggaatcg aacccgcgat
      841 cttcgccgtg acagggcgac gtgataaccg ctacactacg ggacctgtat tttccaatac
//
LOCUS       DATXIG010000145          859 bp    DNA     linear   BCT 28-DEC-2023
DEFINITION  TPA_asm: Streptococcus pyogenes strain MGAS11113
            SAMN08555799-rid19682583.denovo.145, whole genome shotgun sequence.
ACCESSION   DATXIG010000145 DATXIG010000000
VERSION     DATXIG010000145.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN08555799
            Sequence Read Archive: SRR6740495
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pyogenes
  ORGANISM  Streptococcus pyogenes
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 859)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 859)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-OCT-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-OCT-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001918058.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001918058.2
            Genome Coverage       :: 368x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/12/2023 11:23:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 1,806
            CDSs (total)                      :: 1,769
            Genes (coding)                    :: 1,722
            CDSs (with protein)               :: 1,722
            Genes (RNA)                       :: 37
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1, 1 (5S, 23S)
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 47
            CDSs (without protein)            :: 47
            Pseudo Genes (ambiguous residues) :: 0 of 47
            Pseudo Genes (frameshifted)       :: 30 of 47
            Pseudo Genes (incomplete)         :: 20 of 47
            Pseudo Genes (internal stop)      :: 9 of 47
            Pseudo Genes (multiple problems)  :: 11 of 47
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..859
                     /organism="Streptococcus pyogenes"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN08555799-rid19682583.denovo.145"
                     /strain="MGAS11113"
                     /serotype="Emm28"
                     /isolation_source="normally sterile site"
                     /host="Homo sapiens"
                     /db_xref="taxon:1314"
                     /country="Finland"
                     /collection_date="2002"
                     /collected_by="National Public Health Laboratory"
     gene            <1..>859
                     /locus_tag="VC122_001762"
     CDS             <1..>859
                     /locus_tag="VC122_001762"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002990050.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEP5641646.1"
                     /translation="AKASFFKLSSSKESNLKLFIEKMEEMFDNFRKKQYSNIPTLEIN
                     DLMYYINAMQKVTSEEELNGTNLFYWLVTISRVDTESPIILANLEKNIDVRKREIEHG
                     DNEGLVVDTRLLFDPFRQILVVYNQRGTINNYDLRRFFCQIIGVRGLKFDIILNSDAF
                     KRVGKLDVVNSISYTVASPTNFKEFRDDTQSENADLKFANSMLGESMQVVIKSNHLSK
                     KNIFDKFSDMLVNDSVNVKNAKVEGLTDGHPELIDLIKNKLEYKGTIFYENTLDDEAV
                     YAFLNTAYS"
BASE COUNT          324 a           92 c          159 g          284 t
ORIGIN      
        1 ccgcaaaagc aagtttcttt aaattaagtt cttctaaaga atctaattta aaattgttta
       61 tcgaaaagat ggaggaaatg tttgataatt ttagaaaaaa acaatatagt aacattccaa
      121 cattagagat aaatgatctt atgtattata taaatgcaat gcagaaggta acaagtgaag
      181 aagaattaaa tggaacgaat ctattttatt ggttagttac catttcacgg gtggatacag
      241 aatctccaat tattcttgcg aatcttgaaa aaaatattga tgtaagaaaa agggaaattg
      301 aacatgggga taatgagggc cttgtggttg atacaagatt attatttgac ccatttagac
      361 aaattttagt tgtttataat caaagaggta caattaataa ttacgactta agaaggtttt
      421 tttgtcaaat aattggagtt agaggattga agtttgatat tatattaaac agtgatgctt
      481 tcaaaagggt tggtaagctt gatgttgtaa attcaattag ttatactgtt gcaagcccaa
      541 caaactttaa ggagtttcga gatgatactc aaagtgaaaa tgccgattta aagtttgcaa
      601 attctatgct aggagagtca atgcaggttg ttataaagtc aaaccatctt tctaaaaaaa
      661 atatatttga taaattttca gatatgttag ttaatgactc agttaatgta aaaaatgcta
      721 aagttgaagg gcttaccgat ggtcatccgg aattaataga cttaataaaa aataaattgg
      781 aatataaggg aactattttt tatgaaaata ctttagacga tgaggctgtt tatgcatttc
      841 tgaataccgc gtatagctt
//
LOCUS       DATXIG010000146          834 bp    DNA     linear   BCT 28-DEC-2023
DEFINITION  TPA_asm: Streptococcus pyogenes strain MGAS11113
            SAMN08555799-rid19682583.denovo.146, whole genome shotgun sequence.
ACCESSION   DATXIG010000146 DATXIG010000000
VERSION     DATXIG010000146.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN08555799
            Sequence Read Archive: SRR6740495
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pyogenes
  ORGANISM  Streptococcus pyogenes
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 834)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 834)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-OCT-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-OCT-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001918058.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001918058.2
            Genome Coverage       :: 368x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/12/2023 11:23:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 1,806
            CDSs (total)                      :: 1,769
            Genes (coding)                    :: 1,722
            CDSs (with protein)               :: 1,722
            Genes (RNA)                       :: 37
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1, 1 (5S, 23S)
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 47
            CDSs (without protein)            :: 47
            Pseudo Genes (ambiguous residues) :: 0 of 47
            Pseudo Genes (frameshifted)       :: 30 of 47
            Pseudo Genes (incomplete)         :: 20 of 47
            Pseudo Genes (internal stop)      :: 9 of 47
            Pseudo Genes (multiple problems)  :: 11 of 47
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..834
                     /organism="Streptococcus pyogenes"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN08555799-rid19682583.denovo.146"
                     /strain="MGAS11113"
                     /serotype="Emm28"
                     /isolation_source="normally sterile site"
                     /host="Homo sapiens"
                     /db_xref="taxon:1314"
                     /country="Finland"
                     /collection_date="2002"
                     /collected_by="National Public Health Laboratory"
     gene            24..806
                     /locus_tag="VC122_001763"
     CDS             24..806
                     /locus_tag="VC122_001763"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003057468.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transporter substrate-binding domain-containing
                     protein"
                     /protein_id="HEP5641647.1"
                     /translation="MLCLLLYPLAACNKSEQLNHYERIKKTRKLVVAVSPDYAPFEFK
                     ALVNGKDTIVGADVQLAQAIADELDVDLELSPMSFDNVLSSLQTGKADLAISGISHTK
                     ERAKVYDFSIPYYQAENAIVMRASDAKVTKNISDLNGKKVAAQKGSIEEGLVKIQLPK
                     ANLISLTAMGEAINELKAGQVYAVTLEAPVAAGFLAQHKDLALAPFSLKTSDGDAKAV
                     ALPKNSGDLTKAVNKVIAKLDEQERYKSFIAETIALTKNTMK"
BASE COUNT          289 a          134 c          160 g          251 t
ORIGIN      
        1 ttaaaaaaat aagatttggt tttttgcttt gcctcctact ttatccttta gcagcttgta
       61 ataagtctga acaacttaat cactacgaaa gaattaaaaa aacaagaaaa ttagtcgttg
      121 ctgtaagccc tgattatgct ccttttgaat ttaaagcttt agttaatgga aaagatacta
      181 ttgttggagc agatgttcaa ttagctcagg ctattgctga tgaattagac gttgatcttg
      241 agctttcacc aatgagtttt gataatgttt tgtctagttt acagactggt aaagctgatc
      301 ttgctatttc aggaatctca catactaaag aaagagctaa agtatatgat ttttcaattc
      361 cttactatca ggcagaaaac gctatagtga tgagagcatc tgatgctaaa gtgaccaaaa
      421 atattagtga cctaaacggt aagaaagtag ccgctcaaaa aggtagtatc gaggaaggtt
      481 tagttaaaat acaattacca aaggccaatc tgatttcttt gactgctatg ggagaagcca
      541 ttaatgaact aaaagcagga caagtctatg cagttacctt agaagcacct gtagctgctg
      601 gttttttagc ccaacataag gatctggctt tagcaccctt tagcttaaaa accagtgatg
      661 gagatgccaa agcagtggcc cttcctaaaa atagtggtga cttaaccaaa gccgttaata
      721 aggttattgc taagttagat gagcaagaac ggtacaagtc atttattgca gagacaatag
      781 cactaacgaa aaatactatg aagtaataag gttaataaaa actttattag tatc
//
LOCUS       DATXIG010000147          825 bp    DNA     linear   BCT 28-DEC-2023
DEFINITION  TPA_asm: Streptococcus pyogenes strain MGAS11113
            SAMN08555799-rid19682583.denovo.147, whole genome shotgun sequence.
ACCESSION   DATXIG010000147 DATXIG010000000
VERSION     DATXIG010000147.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN08555799
            Sequence Read Archive: SRR6740495
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pyogenes
  ORGANISM  Streptococcus pyogenes
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 825)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 825)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-OCT-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-OCT-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001918058.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001918058.2
            Genome Coverage       :: 368x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/12/2023 11:23:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 1,806
            CDSs (total)                      :: 1,769
            Genes (coding)                    :: 1,722
            CDSs (with protein)               :: 1,722
            Genes (RNA)                       :: 37
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1, 1 (5S, 23S)
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 47
            CDSs (without protein)            :: 47
            Pseudo Genes (ambiguous residues) :: 0 of 47
            Pseudo Genes (frameshifted)       :: 30 of 47
            Pseudo Genes (incomplete)         :: 20 of 47
            Pseudo Genes (internal stop)      :: 9 of 47
            Pseudo Genes (multiple problems)  :: 11 of 47
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..825
                     /organism="Streptococcus pyogenes"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN08555799-rid19682583.denovo.147"
                     /strain="MGAS11113"
                     /serotype="Emm28"
                     /isolation_source="normally sterile site"
                     /host="Homo sapiens"
                     /db_xref="taxon:1314"
                     /country="Finland"
                     /collection_date="2002"
                     /collected_by="National Public Health Laboratory"
     gene            95..165
                     /locus_tag="VC122_001764"
     tRNA            95..165
                     /locus_tag="VC122_001764"
                     /product="tRNA-Thr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:127..129,aa:Thr,seq:ggt)
     gene            complement(241..780)
                     /locus_tag="VC122_001765"
     CDS             complement(241..780)
                     /locus_tag="VC122_001765"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000891237.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific integrase"
                     /protein_id="HEP5641648.1"
                     /translation="MLQTGFRFGEAMAITWDDIDFERNSIYTYRRYSSIKKDFTKPKT
                     RTSIRRVPVSHDLKELLLELKFQQERMLRGLHIKNTDQLIFYDYRFGMISNNAVNKFL
                     ASSLEKLEIESKMSSTGARHTYGSYLLANGVDIWAVAKLMGHKDIKQLIETYGHLLLE
                     VEEKENDQLLKILNNKGSF"
BASE COUNT          266 a          137 c          109 g          313 t
ORIGIN      
        1 taactgatac tctcattttg atgataaaca attaaggaaa tatttttaag aaaagcttgc
       61 atcaattaat aacttatagt attataagaa ggtagccgct gtagttaaat ggtataataa
      121 agcaatggta atgcttcgtt ccgagttcga ttctcggtgg tggcagaaaa tatacgagtg
      181 aaaccttatt ataatagggt ttcttttatt tttgttctta atttgttctt gatagtcatt
      241 ttaaaaacta cctttattat taagaatttt taaaagctga tcattttcct tttcttccac
      301 ctccagaagc aaatgaccat atgtttctat taattgtttg atatctttat gccccattaa
      361 ttttgcaacc gcccaaatat ctactccgtt agccaacaaa taacttccat aagtatgtct
      421 agcacctgtt gatgacatct tagattctat ttctaatttt tccaatgagc ttgccaagaa
      481 tttgttaaca gcattatttg atatcatacc aaacctatag tcataaaaaa tcaattgatc
      541 tgtatttttt atatgcaaac ctctcagcat tctttcttgt tgaaatttta gttcaagcaa
      601 taactctttt aaatcatgac ttactggaac tctccttatt gaagtccttg ttttaggctt
      661 tgtaaagtcc ttttttattg aagaatatct tcgatatgta taaatactat ttctttcaaa
      721 atcaatatca tcccaagtta ttgccattgc ttcaccaaac ctaaaaccag tttgtaacat
      781 tgtgaaaaga aaaaaaggga ttattgagtc tgaataatta gctat
//
LOCUS       DATXIG010000148          807 bp    DNA     linear   BCT 28-DEC-2023
DEFINITION  TPA_asm: Streptococcus pyogenes strain MGAS11113
            SAMN08555799-rid19682583.denovo.148, whole genome shotgun sequence.
ACCESSION   DATXIG010000148 DATXIG010000000
VERSION     DATXIG010000148.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN08555799
            Sequence Read Archive: SRR6740495
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pyogenes
  ORGANISM  Streptococcus pyogenes
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 807)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 807)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-OCT-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-OCT-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001918058.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001918058.2
            Genome Coverage       :: 368x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/12/2023 11:23:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 1,806
            CDSs (total)                      :: 1,769
            Genes (coding)                    :: 1,722
            CDSs (with protein)               :: 1,722
            Genes (RNA)                       :: 37
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1, 1 (5S, 23S)
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 47
            CDSs (without protein)            :: 47
            Pseudo Genes (ambiguous residues) :: 0 of 47
            Pseudo Genes (frameshifted)       :: 30 of 47
            Pseudo Genes (incomplete)         :: 20 of 47
            Pseudo Genes (internal stop)      :: 9 of 47
            Pseudo Genes (multiple problems)  :: 11 of 47
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..807
                     /organism="Streptococcus pyogenes"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN08555799-rid19682583.denovo.148"
                     /strain="MGAS11113"
                     /serotype="Emm28"
                     /isolation_source="normally sterile site"
                     /host="Homo sapiens"
                     /db_xref="taxon:1314"
                     /country="Finland"
                     /collection_date="2002"
                     /collected_by="National Public Health Laboratory"
     gene            130..>807
                     /locus_tag="VC122_001766"
     CDS             130..>807
                     /locus_tag="VC122_001766"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010922274.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lantibiotic protection ABC transporter
                     ATP-binding protein"
                     /protein_id="HEP5641649.1"
                     /translation="MLKIQNLKKSYGKRTILNNVNMNIPKGKVYALIGPNGAGKSTIM
                     KILTGLVSKTSGSIIFEGREWSRRDLRKIGSIIEEPPLYKNLSAYDNMKVVTTMLGVS
                     ESAILPLLNKVGLGNIDKRPVKQFSLGMKQRLGIAISLINSPKLLILDEPTNGLDPIG
                     IQELREIIESFKSEGMTIMISSHILSEVEHLADFIGFIYEGKIVLEKEYDGSENLEEL
                     FNNQILFE"
BASE COUNT          299 a           99 c          156 g          253 t
ORIGIN      
        1 agtaggaagt tttttacatc tatcaatgaa tcgattaaac gggattgata gagagtttga
       61 agagaaagtg tattgttttg cttactatac actaaacgct cagcaatata tagaatggga
      121 gtaactaata tgttgaaaat acaaaatcta aaaaaatctt atggtaagag aaccatattg
      181 aataatgtca atatgaatat tcctaaggga aaagtgtatg ctttaatcgg tccaaatggt
      241 gctggaaaat caactattat gaaaattttg acaggcttag ttagtaagac aagtggttct
      301 attatttttg aaggtagaga atggtcacgt cgggatctgc gaaaaatcgg gagtattatt
      361 gaagaaccac cactttataa aaatttgagt gcttacgata atatgaaggt agttacaaca
      421 atgcttggtg tttcagaaag cgctatactt ccattattaa ataaagttgg tctaggaaat
      481 attgacaaga gaccagtaaa acaattttct cttggaatga agcaacggtt aggtatagct
      541 atctctttaa taaattcacc taaattactt atattagacg aacctactaa tggcttggac
      601 ccaattggaa ttcaagaatt aagggaaatt atagagtcat ttaaatcaga aggaatgaca
      661 attatgattt caagccatat actgtcagaa gttgaacatc tagctgattt tattggattt
      721 atctatgaag gaaagattgt tctggaaaaa gaatatgacg gctctgagaa tcttgaagag
      781 ttattcaata atcaaatttt atttgaa
//
LOCUS       DATXIG010000149          797 bp    DNA     linear   BCT 28-DEC-2023
DEFINITION  TPA_asm: Streptococcus pyogenes strain MGAS11113
            SAMN08555799-rid19682583.denovo.149, whole genome shotgun sequence.
ACCESSION   DATXIG010000149 DATXIG010000000
VERSION     DATXIG010000149.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN08555799
            Sequence Read Archive: SRR6740495
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pyogenes
  ORGANISM  Streptococcus pyogenes
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 797)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 797)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-OCT-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-OCT-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001918058.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001918058.2
            Genome Coverage       :: 368x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/12/2023 11:23:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 1,806
            CDSs (total)                      :: 1,769
            Genes (coding)                    :: 1,722
            CDSs (with protein)               :: 1,722
            Genes (RNA)                       :: 37
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1, 1 (5S, 23S)
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 47
            CDSs (without protein)            :: 47
            Pseudo Genes (ambiguous residues) :: 0 of 47
            Pseudo Genes (frameshifted)       :: 30 of 47
            Pseudo Genes (incomplete)         :: 20 of 47
            Pseudo Genes (internal stop)      :: 9 of 47
            Pseudo Genes (multiple problems)  :: 11 of 47
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..797
                     /organism="Streptococcus pyogenes"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN08555799-rid19682583.denovo.149"
                     /strain="MGAS11113"
                     /serotype="Emm28"
                     /isolation_source="normally sterile site"
                     /host="Homo sapiens"
                     /db_xref="taxon:1314"
                     /country="Finland"
                     /collection_date="2002"
                     /collected_by="National Public Health Laboratory"
     gene            49..>797
                     /gene="mf2"
                     /locus_tag="VC122_001767"
     CDS             49..>797
                     /gene="mf2"
                     /locus_tag="VC122_001767"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002985324.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNase Mf2"
                     /protein_id="HEP5641650.1"
                     /translation="MKLSKQKASLLTAVLLLLSLSITTITVDAAKVRTYPNVSHANTH
                     YKNTASSKLLPFTANYQLQLGELDNLNRATFSHIQLQDRHETKDVRTKINYDPVGWHN
                     YQFPYGDGSKSSWVMNRGHLVGYQFCGLNDEPRNLVTMTAWLNTGAYSGANDSNPEGM
                     LYYENRLDSWLALHPDFWLDYKVTPIYSGNEVVPRQIELQYVGIDSSGELLTIRLNSN
                     KESIDENGVTTVILENSAPNINLDYLNGTATP"
BASE COUNT          265 a          148 c          143 g          241 t
ORIGIN      
        1 tatttgatcc actctaaaag ctaaaaatgg aaaggtttag gtgttatcat gaaattatct
       61 aaacaaaagg caagtttgct taccgctgtt ttactgttac tttctctttc tataacgaca
      121 ataactgttg acgcagctaa ggtacgaact tatccgaatg tatcccatgc aaacacacat
      181 tataaaaata ctgcttctag taagctctta ccttttactg ctaattatca gttacaactt
      241 ggtgagttgg ataaccttaa ccgcgctacc ttttcacata tccaactgca agatagacac
      301 gaaactaaag atgtacgcac taaaataaat tatgaccctg tgggatggca taactatcaa
      361 tttccatacg gagatggctc aaaatcttca tgggtaatga atcgtgggca tctagtcgga
      421 tatcaatttt gtggattaaa cgatgaacca agaaatttag tgacaatgac agcctggcta
      481 aacacaggtg cttattcagg agcaaatgac agcaatcctg aagggatgtt atactatgaa
      541 aaccgtttag attcttggct tgcacttcac cctgactttt ggctagatta taaagtcact
      601 cctatatata gtggcaatga agtagtgcct cgacaaattg aattacagta tgttggaatt
      661 gattcatctg gtgagttgct tactataagg ctaaatagta ataaagaaag tattgatgaa
      721 aatggtgtta ctacagtaat cttagaaaac tctgctccaa atatcaacct tgactattta
      781 aatggaactg ccactcc
//
LOCUS       DATXIG010000150          761 bp    DNA     linear   BCT 28-DEC-2023
DEFINITION  TPA_asm: Streptococcus pyogenes strain MGAS11113
            SAMN08555799-rid19682583.denovo.150, whole genome shotgun sequence.
ACCESSION   DATXIG010000150 DATXIG010000000
VERSION     DATXIG010000150.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN08555799
            Sequence Read Archive: SRR6740495
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pyogenes
  ORGANISM  Streptococcus pyogenes
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 761)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 761)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-OCT-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-OCT-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001918058.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001918058.2
            Genome Coverage       :: 368x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/12/2023 11:23:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 1,806
            CDSs (total)                      :: 1,769
            Genes (coding)                    :: 1,722
            CDSs (with protein)               :: 1,722
            Genes (RNA)                       :: 37
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1, 1 (5S, 23S)
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 47
            CDSs (without protein)            :: 47
            Pseudo Genes (ambiguous residues) :: 0 of 47
            Pseudo Genes (frameshifted)       :: 30 of 47
            Pseudo Genes (incomplete)         :: 20 of 47
            Pseudo Genes (internal stop)      :: 9 of 47
            Pseudo Genes (multiple problems)  :: 11 of 47
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..761
                     /organism="Streptococcus pyogenes"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN08555799-rid19682583.denovo.150"
                     /strain="MGAS11113"
                     /serotype="Emm28"
                     /isolation_source="normally sterile site"
                     /host="Homo sapiens"
                     /db_xref="taxon:1314"
                     /country="Finland"
                     /collection_date="2002"
                     /collected_by="National Public Health Laboratory"
     gene            complement(<1..721)
                     /locus_tag="VC122_001768"
     CDS             complement(<1..721)
                     /locus_tag="VC122_001768"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012560530.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S-adenosylmethionine synthetase"
                     /protein_id="HEP5641651.1"
                     /translation="MDLKITNGFYDPSHLSYEVVERKGLGHPDTLADGIAEQIEIDYS
                     LYCLDKFGVIPHHNFDKIIIRGGHSVQDFGGSDFIEPIKIIFLGRASKKCFNSSIPLF
                     KIQKKAATKYLNRILPNLDVENYVEFETLTSDFTTKTNWFSPEAIEDLPEYLDVPKAN
                     DTATMISYWPLTISEELALMIEGYFYKLDKNELPTPRFTKMGGDIKVMVVRNDLEYSI
                     RINFPLISKFFNNDIESQLYVD"
BASE COUNT          259 a          137 c           88 g          277 t
ORIGIN      
        1 tatcaacata caactgactt tcaatatcat tgttaaaaaa tttacttatt aaaggaaaat
       61 ttattctaat agaatattct aaatcgttcc ttacgaccat tactttaata tcaccaccca
      121 ttttagtaaa tcttggcgtt ggtaattcat tcttatctaa tttataaaaa tacccttcaa
      181 tcattaatgc taattcttca gaaattgtta atggccaata acttatcatt gtagctgtat
      241 catttgcctt tggtacatct agatactccg gtaaatcttc tattgcttca ggcgagaacc
      301 aattagtttt tgtagtaaaa tctgaggtca atgtctcaaa ttctacataa ttttcaacat
      361 ctaaattagg taaaattcta tttaaatatt tagttgcagc ctttttctga attttaaaaa
      421 gtggaattga actattaaaa cattttttgc tcgccctacc tagaaaaata attttaattg
      481 gttcaataaa gtcacttcca ccaaaatcct gaactgaatg tccccctcgg ataataatct
      541 tatcaaaatt atgatgtgga ataacaccaa acttgtccaa acaataaagt gagtaatcaa
      601 tttcaatttg ctcagcaatt ccatctgcta aggtatcagg atgccctaat cctttacgtt
      661 ctactacttc gtatgataaa tgacttgggt cataaaagcc gtttgtaatt tttaaatcca
      721 tagttacacc tcataaataa attttttcac attctgaata t
//
LOCUS       DATXIG010000151          752 bp    DNA     linear   BCT 28-DEC-2023
DEFINITION  TPA_asm: Streptococcus pyogenes strain MGAS11113
            SAMN08555799-rid19682583.denovo.151, whole genome shotgun sequence.
ACCESSION   DATXIG010000151 DATXIG010000000
VERSION     DATXIG010000151.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN08555799
            Sequence Read Archive: SRR6740495
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pyogenes
  ORGANISM  Streptococcus pyogenes
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 752)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 752)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-OCT-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-OCT-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001918058.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001918058.2
            Genome Coverage       :: 368x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/12/2023 11:23:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 1,806
            CDSs (total)                      :: 1,769
            Genes (coding)                    :: 1,722
            CDSs (with protein)               :: 1,722
            Genes (RNA)                       :: 37
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1, 1 (5S, 23S)
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 47
            CDSs (without protein)            :: 47
            Pseudo Genes (ambiguous residues) :: 0 of 47
            Pseudo Genes (frameshifted)       :: 30 of 47
            Pseudo Genes (incomplete)         :: 20 of 47
            Pseudo Genes (internal stop)      :: 9 of 47
            Pseudo Genes (multiple problems)  :: 11 of 47
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..752
                     /organism="Streptococcus pyogenes"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN08555799-rid19682583.denovo.151"
                     /strain="MGAS11113"
                     /serotype="Emm28"
                     /isolation_source="normally sterile site"
                     /host="Homo sapiens"
                     /db_xref="taxon:1314"
                     /country="Finland"
                     /collection_date="2002"
                     /collected_by="National Public Health Laboratory"
     gene            63..683
                     /locus_tag="VC122_001769"
     CDS             63..683
                     /locus_tag="VC122_001769"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014407631.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3862 domain-containing protein"
                     /protein_id="HEP5641652.1"
                     /translation="MTKKHLLTLLLISFFTSFLVACSTTKDKEPQPSDSEIITPRLHQ
                     AAHQDKRANFEKIKLATVDSSFTGGTSLEELISLFGEPSQHDPKTAGEVTIDAYTWQF
                     DQVTLTVNLYQNSSIVKTISNFTFARELGLSQKEYQQLQKGMSYEDVKKILTEPDNYS
                     QASSSDHQTLQAIWVSGLKTDTSGANISLVFENNQLTEMSQVGLEE"
BASE COUNT          271 a          136 c          118 g          227 t
ORIGIN      
        1 atagctatat cttgtttata atttatgata taatacagca aaaatagttt aggaaataac
       61 atatgacaaa aaaacactta ctaacacttc ttctcatctc tttttttact agctttttgg
      121 tagcttgttc aacaacgaaa gataaagagc ctcaaccgtc tgattcagaa atcattactc
      181 cccgactaca ccaagccgct catcaagata aacgcgctaa ctttgaaaaa attaaacttg
      241 cgactgttga ttcctcattt acaggaggga caagccttga agaacttatt tcactctttg
      301 gagagcctag ccaacatgat ccaaaaacag caggcgaagt aacaatcgac gcttatactt
      361 ggcagtttga tcaagttact ctcactgtta atctttatca aaatagtagt attgttaaaa
      421 ccatctctaa ttttaccttt gcaagagagt taggcttatc gcaaaaggaa taccaacaat
      481 tacaaaaagg aatgtcttat gaagacgtta aaaagatctt aacagaacct gataattata
      541 gccaagcgtc atctagtgat catcaaactt tgcaagcgat ttgggttagt ggcttaaaga
      601 cagatacaag cggagctaat atttctctcg tttttgaaaa taatcagtta acagaaatgt
      661 ctcaggtagg acttgaagaa taaaaaatga gtaatatatt ttgctaaaaa gattgatttc
      721 gatgtacacc gaaatcaatc tttttatatc at
//
LOCUS       DATXIG010000152          722 bp    DNA     linear   BCT 28-DEC-2023
DEFINITION  TPA_asm: Streptococcus pyogenes strain MGAS11113
            SAMN08555799-rid19682583.denovo.152, whole genome shotgun sequence.
ACCESSION   DATXIG010000152 DATXIG010000000
VERSION     DATXIG010000152.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN08555799
            Sequence Read Archive: SRR6740495
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pyogenes
  ORGANISM  Streptococcus pyogenes
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 722)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 722)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-OCT-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-OCT-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001918058.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001918058.2
            Genome Coverage       :: 368x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/12/2023 11:23:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 1,806
            CDSs (total)                      :: 1,769
            Genes (coding)                    :: 1,722
            CDSs (with protein)               :: 1,722
            Genes (RNA)                       :: 37
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1, 1 (5S, 23S)
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 47
            CDSs (without protein)            :: 47
            Pseudo Genes (ambiguous residues) :: 0 of 47
            Pseudo Genes (frameshifted)       :: 30 of 47
            Pseudo Genes (incomplete)         :: 20 of 47
            Pseudo Genes (internal stop)      :: 9 of 47
            Pseudo Genes (multiple problems)  :: 11 of 47
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..722
                     /organism="Streptococcus pyogenes"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN08555799-rid19682583.denovo.152"
                     /strain="MGAS11113"
                     /serotype="Emm28"
                     /isolation_source="normally sterile site"
                     /host="Homo sapiens"
                     /db_xref="taxon:1314"
                     /country="Finland"
                     /collection_date="2002"
                     /collected_by="National Public Health Laboratory"
     gene            complement(36..>722)
                     /locus_tag="VC122_001770"
     CDS             complement(36..>722)
                     /locus_tag="VC122_001770"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002984791.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lantibiotic immunity ABC transporter MutE/EpiE
                     family permease subunit"
                     /protein_id="HEP5641653.1"
                     /translation="LIFPIICVMLSLLFSALGGESILRLAAETTVNQWGVIWINVIIA
                     VNSGLLNKLELDSNKYSVFLSRDVDLKKVEYARVLLIALINLIISMILSLMLIVISFV
                     LPTPSLISIGRILLTILLIWLTTLWQIPFILWLSRKINVYFAMIINIISPLIIGTSFS
                     LLNKWYLFPYDWSLKLLEPMTRMRINSIPFGAEFVPDYSQIFISLFLGIAFFILLTNL
                     FAISFKKQVK"
BASE COUNT          299 a          118 c           91 g          214 t
ORIGIN      
        1 ttttaaaaaa ttagaacata aatatgattt catgactatt tcacctgttt tttgaaagag
       61 atagcaaata gattggtcag taagatgaaa aaagcaattc ctaggaacaa tgatataaaa
      121 atctgtgagt agtctggaac aaactccgct ccaaaaggta tactatttat tctcattctt
      181 gtcattggct caagcaactt caacgaccaa tcataaggga acaaatacca tttatttaag
      241 agagaaaaac ttgtaccaat aattagtgga gatataatat taataatcat agcaaaatac
      301 acattaattt ttcttgatag ccataaaata aacgggattt gccatagtgt agtcaaccaa
      361 attaacaaga tagttaatag tatcctacct atactaataa gacttggagt aggtaggaca
      421 aaactaatca caataagcat taagcttaaa atcatactaa ttattagatt tataagagct
      481 atcaatagaa ctctggcata ttctaccttt tttaaatcaa catcccttga taaaaaaaca
      541 gaatacttat tagaatctaa ttctaattta tttaacaaac cagaatttac agcaataata
      601 acattaatcc aaataactcc ccactgattt actgttgttt ccgcagctaa acgtaggata
      661 ctttcaccac ctaatgctga aaacaataaa gataacatca cacaaataat aggaaaaata
      721 ag
//
LOCUS       DATXIG010000153          710 bp    DNA     linear   BCT 28-DEC-2023
DEFINITION  TPA_asm: Streptococcus pyogenes strain MGAS11113
            SAMN08555799-rid19682583.denovo.153, whole genome shotgun sequence.
ACCESSION   DATXIG010000153 DATXIG010000000
VERSION     DATXIG010000153.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN08555799
            Sequence Read Archive: SRR6740495
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pyogenes
  ORGANISM  Streptococcus pyogenes
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 710)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 710)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-OCT-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-OCT-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001918058.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001918058.2
            Genome Coverage       :: 368x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/12/2023 11:23:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 1,806
            CDSs (total)                      :: 1,769
            Genes (coding)                    :: 1,722
            CDSs (with protein)               :: 1,722
            Genes (RNA)                       :: 37
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1, 1 (5S, 23S)
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 47
            CDSs (without protein)            :: 47
            Pseudo Genes (ambiguous residues) :: 0 of 47
            Pseudo Genes (frameshifted)       :: 30 of 47
            Pseudo Genes (incomplete)         :: 20 of 47
            Pseudo Genes (internal stop)      :: 9 of 47
            Pseudo Genes (multiple problems)  :: 11 of 47
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..710
                     /organism="Streptococcus pyogenes"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN08555799-rid19682583.denovo.153"
                     /strain="MGAS11113"
                     /serotype="Emm28"
                     /isolation_source="normally sterile site"
                     /host="Homo sapiens"
                     /db_xref="taxon:1314"
                     /country="Finland"
                     /collection_date="2002"
                     /collected_by="National Public Health Laboratory"
     gene            complement(<1..534)
                     /locus_tag="VC122_001771"
     CDS             complement(<1..534)
                     /locus_tag="VC122_001771"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002990276.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipopolysaccharide biosynthesis protein"
                     /protein_id="HEP5641654.1"
                     /translation="MINPSKQNQTIFLWNMLGSLSTAVISVILLMVVTRLLTSADSDI
                     YAFAYSFANMMVVVGLFQVRNYQATDINEKYSFSQYLVARLMTCLLMLAITVIYLTLT
                     KTDSYKSTIVFLVCFYRSTDAFSDLYQGMFQQHERLDIAGKSLAYRNTLIFMVYTAII
                     LYSKNLTLALVAVCIVSL"
     gene            complement(527..>710)
                     /locus_tag="VC122_001772"
     CDS             complement(527..>710)
                     /locus_tag="VC122_001772"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002985167.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="DUF2304 domain-containing protein"
                     /protein_id="HEP5641655.1"
                     /translation="LNKMSHFLGIHSPTNMLFFLGFCLSLAIIFSLTNNISLQNDKVK
                     RLTQEVALMKKEKTND"
BASE COUNT          260 a          112 c          110 g          228 t
ORIGIN      
        1 taatgaaact atacagacag ctactaatgc taaagtaaga tttttggaat ataatattat
       61 tgctgtgtac accataaaaa taagagtatt tctgtaggct agcgatttac ctgcaatatc
      121 cagccgttca tgttgttgga acattccctg atataaatcc gaaaaagcat ctgtcgatct
      181 atagaaacag actagaaaga caattgtact cttgtagcta tctgtcttag ttaacgttaa
      241 ataaattact gttatggcta acattaataa acaagtcatc agtcttgcca ctaagtactg
      301 actaaaggaa tatttttcat taatatctgt tgcttgataa ttacgaacct gaaaaagtcc
      361 aacaactacc atcatattag caaaagaata ggcaaaggcg taaatatctg aatcagccga
      421 agttagtaac ctagtaacca ccataagtaa tataactgat attacagctg ttgataaact
      481 tcctaacata ttccataaaa aaatcgtttg gttttgtttt gaaggattaa tcattggttt
      541 tctctttttt cataagggcc acttcttggg tcagacgttt tactttatca ttttgaagtg
      601 aaatattatt agtcagtgaa aaaataatag ctaaactcaa acaaaaacct aaaaagaata
      661 acatattggt tggtgaatga atacccagga agtgagacat tttatttaat
//
LOCUS       DATXIG010000154          678 bp    DNA     linear   BCT 28-DEC-2023
DEFINITION  TPA_asm: Streptococcus pyogenes strain MGAS11113
            SAMN08555799-rid19682583.denovo.154, whole genome shotgun sequence.
ACCESSION   DATXIG010000154 DATXIG010000000
VERSION     DATXIG010000154.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN08555799
            Sequence Read Archive: SRR6740495
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pyogenes
  ORGANISM  Streptococcus pyogenes
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 678)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 678)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-OCT-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-OCT-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001918058.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001918058.2
            Genome Coverage       :: 368x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/12/2023 11:23:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 1,806
            CDSs (total)                      :: 1,769
            Genes (coding)                    :: 1,722
            CDSs (with protein)               :: 1,722
            Genes (RNA)                       :: 37
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1, 1 (5S, 23S)
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 47
            CDSs (without protein)            :: 47
            Pseudo Genes (ambiguous residues) :: 0 of 47
            Pseudo Genes (frameshifted)       :: 30 of 47
            Pseudo Genes (incomplete)         :: 20 of 47
            Pseudo Genes (internal stop)      :: 9 of 47
            Pseudo Genes (multiple problems)  :: 11 of 47
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..678
                     /organism="Streptococcus pyogenes"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN08555799-rid19682583.denovo.154"
                     /strain="MGAS11113"
                     /serotype="Emm28"
                     /isolation_source="normally sterile site"
                     /host="Homo sapiens"
                     /db_xref="taxon:1314"
                     /country="Finland"
                     /collection_date="2002"
                     /collected_by="National Public Health Laboratory"
     gene            <1..524
                     /locus_tag="VC122_001773"
     CDS             <1..524
                     /locus_tag="VC122_001773"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002989896.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="lanthionine synthetase"
                     /protein_id="HEP5641656.1"
                     /translation="NLMFLFRRDDKLWPGKIYTSNILDLDLNYLPTRMAWCYGTPGTA
                     VALFKMCQLIDLNFIDDINESLIAQMRSSDKNVYSLTMCHGLAGVTFIYDLIGKNYSS
                     ERLTRFAEELRQQIITSFSTTKVFGYTDREKFGEIEVELESIGILQGVSGIVLFLLNP
                     YSDEELLWQRIFI"
     gene            543..>678
                     /locus_tag="VC122_001774"
     CDS             543..>678
                     /locus_tag="VC122_001774"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002989894.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEP5641657.1"
                     /translation="MYKVFPTGMIRIPLQKQEFYNKDYEDLINDKVFMEQLLVASPSL
                     Y"
BASE COUNT          234 a           81 c          132 g          231 t
ORIGIN      
        1 taaatttaat gtttttattt agaagggatg acaaactatg gcctggaaaa atttatacta
       61 gtaatatttt ggatcttgat ttaaattatc ttcctactag aatggcatgg tgttacggta
      121 cgcctggaac tgcagttgcc ctttttaaaa tgtgtcaatt gatagattta aattttattg
      181 acgatattaa tgaaagttta attgctcaga tgagaagttc agacaaaaat gtctattctc
      241 ttacaatgtg ccatggtttg gcaggagtaa cttttatata tgacttgatt ggtaaaaatt
      301 attcaagtga gaggctaact cgctttgcag aagagcttcg ccaacaaata attacatcgt
      361 tttcaactac aaaagtattt gggtatactg acagagaaaa atttggagaa atagaagttg
      421 aactggagtc aatagggata ttgcaagggg tatcagggat agtgttattc ttattaaatc
      481 cttacagtga cgaggagcta ttgtggcaaa gaatatttat ttaatttgaa aaggagaaca
      541 aaatgtataa ggtatttcca actggtatga ttcgtattcc attgcaaaaa caagaatttt
      601 ataataaaga ctatgaagat ttaataaatg ataaagtttt tatggaacaa cttttagttg
      661 ctagtcccag tctttatg
//
LOCUS       DATXIG010000155          666 bp    DNA     linear   BCT 28-DEC-2023
DEFINITION  TPA_asm: Streptococcus pyogenes strain MGAS11113
            SAMN08555799-rid19682583.denovo.155, whole genome shotgun sequence.
ACCESSION   DATXIG010000155 DATXIG010000000
VERSION     DATXIG010000155.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN08555799
            Sequence Read Archive: SRR6740495
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pyogenes
  ORGANISM  Streptococcus pyogenes
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 666)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 666)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-OCT-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-OCT-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001918058.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001918058.2
            Genome Coverage       :: 368x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/12/2023 11:23:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 1,806
            CDSs (total)                      :: 1,769
            Genes (coding)                    :: 1,722
            CDSs (with protein)               :: 1,722
            Genes (RNA)                       :: 37
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1, 1 (5S, 23S)
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 47
            CDSs (without protein)            :: 47
            Pseudo Genes (ambiguous residues) :: 0 of 47
            Pseudo Genes (frameshifted)       :: 30 of 47
            Pseudo Genes (incomplete)         :: 20 of 47
            Pseudo Genes (internal stop)      :: 9 of 47
            Pseudo Genes (multiple problems)  :: 11 of 47
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..666
                     /organism="Streptococcus pyogenes"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN08555799-rid19682583.denovo.155"
                     /strain="MGAS11113"
                     /serotype="Emm28"
                     /isolation_source="normally sterile site"
                     /host="Homo sapiens"
                     /db_xref="taxon:1314"
                     /country="Finland"
                     /collection_date="2002"
                     /collected_by="National Public Health Laboratory"
     gene            complement(<1..618)
                     /gene="tilS"
                     /locus_tag="VC122_001775"
     CDS             complement(<1..618)
                     /gene="tilS"
                     /locus_tag="VC122_001775"
                     /EC_number="6.3.4.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011054089.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA lysidine(34) synthetase TilS"
                     /protein_id="HEP5641658.1"
                     /translation="MNLLHFLYLFQDKLKIRIGIAHVNHKQRSESDSEEAYLKCWAKK
                     HDIPIYVSNFEGIFSEKAARDWRYAFFKSIMLKNNYSALVTAHHSDDQAETILMRLIR
                     GSRLRHLSGIKSVQPFANGQLIRPFLTFSKKDLPEIFHFEDSSNRELSFLRNRVRNNY
                     LPLLKQENPRFIQGLNQLALENSLLFQAFKELTNHITTTDLTEFNE"
BASE COUNT          220 a          111 c           95 g          240 t
ORIGIN      
        1 ctcattaaat tctgttaaat cagtggtagt aatatgattt gttagctcct taaaagcttg
       61 aaaaagtaag ctattttcaa gtgctaattg atttaatccc tgaatgaacc taggattttc
      121 ctgctttaac aatggtaaat aattatttct aactctattt cttaaaaatg ataattccct
      181 atttgaagaa tcttcaaaat gaaaaatttc tggcaagtcc ttttttgaga aagttaaaaa
      241 aggtcttata agttgaccat tggcaaatgg ttgcacgctt tttatacccg acaagtgcct
      301 caatcggctt cctctaatta gcctcatcaa aatagtttct gcttgatcat ctgaatggtg
      361 agctgttact aaagctgaat agttattttt taacataata cttttaaaaa aagcatagcg
      421 ccaatcacgt gctgcttttt ctgaaaatat tccttcaaaa ttagaaacat aaataggtat
      481 gtcatgtttt ttagcccaac actttaaata agcttcttct gaatctgatt ctgatctttg
      541 tttgtgatta acatgagcaa taccaatacg aattttcaat ttgtcttgaa acaggtataa
      601 aaagtgcaat aaattcattg aatctactcc accagatacg gctattaaca catgtctatg
      661 attttt
//
LOCUS       DATXIG010000156          654 bp    DNA     linear   BCT 28-DEC-2023
DEFINITION  TPA_asm: Streptococcus pyogenes strain MGAS11113
            SAMN08555799-rid19682583.denovo.156, whole genome shotgun sequence.
ACCESSION   DATXIG010000156 DATXIG010000000
VERSION     DATXIG010000156.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN08555799
            Sequence Read Archive: SRR6740495
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pyogenes
  ORGANISM  Streptococcus pyogenes
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 654)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 654)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-OCT-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-OCT-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001918058.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001918058.2
            Genome Coverage       :: 368x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/12/2023 11:23:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 1,806
            CDSs (total)                      :: 1,769
            Genes (coding)                    :: 1,722
            CDSs (with protein)               :: 1,722
            Genes (RNA)                       :: 37
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1, 1 (5S, 23S)
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 47
            CDSs (without protein)            :: 47
            Pseudo Genes (ambiguous residues) :: 0 of 47
            Pseudo Genes (frameshifted)       :: 30 of 47
            Pseudo Genes (incomplete)         :: 20 of 47
            Pseudo Genes (internal stop)      :: 9 of 47
            Pseudo Genes (multiple problems)  :: 11 of 47
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..654
                     /organism="Streptococcus pyogenes"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN08555799-rid19682583.denovo.156"
                     /strain="MGAS11113"
                     /serotype="Emm28"
                     /isolation_source="normally sterile site"
                     /host="Homo sapiens"
                     /db_xref="taxon:1314"
                     /country="Finland"
                     /collection_date="2002"
                     /collected_by="National Public Health Laboratory"
     gene            complement(<1..650)
                     /locus_tag="VC122_001776"
     CDS             complement(<1..650)
                     /locus_tag="VC122_001776"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010922355.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HEP5641659.1"
                     /translation="MIKILLVEDDLSLSNSIFDFLDDFADVMQVFDGDEGLYEAESGI
                     YDLILLDLMLPEKNGFQVLKELREKDIKIPVLIMTAKEGLDDKGHGFELGADDYLTKP
                     FYLEELKMRIQALLKRTGKFADKNISFGNLVVDLARKEVKVEGKVVELLGKEFDLLVY
                     LLQNQNVILPKTQIFDRLWGFDSDTTISVVEVYISKIRKKLKGTRFVNRLQTLRSVG"
BASE COUNT          215 a          143 c           70 g          226 t
ORIGIN      
        1 cctacgcttc ttaaagtttg aagcctgtta acaaaacggg taccttttaa ttttttcctt
       61 attttagaaa tgtaaacttc aacaaccgaa atcgtagtat cactatcgaa tccccataaa
      121 cgatcaaaaa tttgtgtctt tggtaaaata acgttttgat tttgcaaaag atacaccaac
      181 aaatcaaatt ctttgccaag taattcaact actttccctt caaccttcac ctcttttcga
      241 gctaagtcga ccactaaatt gccaaaacta atatttttat ctgcaaattt ccctgttctt
      301 tttaacagtg cttgaattcg catttttaac tcttctaaat agaacggctt tgtcagataa
      361 tcatcagcac ctaattcaaa tccgtgacct ttatcatcta aaccctcttt agctgtcatg
      421 atcaatacag gaattttaat atctttttct cgcaattctt ttaagacttg aaagccattt
      481 ttctccggca acataaggtc taacaaaatc aaatcataaa taccactttc tgcttcgtat
      541 aaaccttcat cgccatcaaa aacttgcatg acgtctgcaa aatcatctaa aaaatcaaaa
      601 atggaatttg ataagctcaa gtcatcttct actaataata tttttatcat gtct
//
LOCUS       DATXIG010000157          629 bp    DNA     linear   BCT 28-DEC-2023
DEFINITION  TPA_asm: Streptococcus pyogenes strain MGAS11113
            SAMN08555799-rid19682583.denovo.157, whole genome shotgun sequence.
ACCESSION   DATXIG010000157 DATXIG010000000
VERSION     DATXIG010000157.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN08555799
            Sequence Read Archive: SRR6740495
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pyogenes
  ORGANISM  Streptococcus pyogenes
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 629)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 629)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-OCT-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-OCT-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001918058.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001918058.2
            Genome Coverage       :: 368x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/12/2023 11:23:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 1,806
            CDSs (total)                      :: 1,769
            Genes (coding)                    :: 1,722
            CDSs (with protein)               :: 1,722
            Genes (RNA)                       :: 37
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1, 1 (5S, 23S)
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 47
            CDSs (without protein)            :: 47
            Pseudo Genes (ambiguous residues) :: 0 of 47
            Pseudo Genes (frameshifted)       :: 30 of 47
            Pseudo Genes (incomplete)         :: 20 of 47
            Pseudo Genes (internal stop)      :: 9 of 47
            Pseudo Genes (multiple problems)  :: 11 of 47
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..629
                     /organism="Streptococcus pyogenes"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN08555799-rid19682583.denovo.157"
                     /strain="MGAS11113"
                     /serotype="Emm28"
                     /isolation_source="normally sterile site"
                     /host="Homo sapiens"
                     /db_xref="taxon:1314"
                     /country="Finland"
                     /collection_date="2002"
                     /collected_by="National Public Health Laboratory"
     gene            complement(154..336)
                     /locus_tag="VC122_001777"
     CDS             complement(154..336)
                     /locus_tag="VC122_001777"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002987564.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class IIb bacteriocin, lactobin A/cerein 7B
                     family"
                     /protein_id="HEP5641660.1"
                     /translation="MDNFLELQFEELVNISGGKGNIGSAIGGCLGGMLIAAAGGPITG
                     GAAAFVCVASGIAAYQ"
     gene            complement(351..584)
                     /locus_tag="VC122_001778"
     CDS             complement(351..584)
                     /locus_tag="VC122_001778"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002994504.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Blp family class II bacteriocin"
                     /protein_id="HEP5641661.1"
                     /translation="MIKFAEEIQKEELFHIIGGYSATDCKNHLIGGITSGAIAGGVGA
                     GMATLGVGGVAGAFAGAIAGGLTCVGGMLFNGK"
BASE COUNT          220 a          153 c           57 g          199 t
ORIGIN      
        1 taatattatt aatttgtaat agtacttagt aagacataaa caaatatcac aattaaaatt
       61 actttacttg cattttctct tacctcttta tccttcaaaa aaatttggga aagtattaga
      121 caaattaaac cgagtattat aactataatc atatcattga taagctgcaa ttccagatgc
      181 tacacataca aatgctgccg cacctccagt tattggacct cctgctgcag ctataagcat
      241 cccacctaag caccctccga tagcagatcc aatatttcct tttccaccag aaatatttac
      301 taattcttca aactgtaatt ctaaaaaatt atccattttt cacctcaaaa ttattttcca
      361 ttaaataaca taccaccaac gcaagttaat ccacccgcaa tagcacctgc aaacgcacct
      421 gcaactcctc caacacctaa agtagccata cctgctccaa caccacctgc aatagcacct
      481 gaagtaatac caccaattaa atgattttta caatctgtag cactataacc tccaataata
      541 tggaacaact cttctttttg tatttcttct gcaaatttta tcataacttt aaaaaccttt
      601 ctaaaatgtt atattcatct tgttgcaac
//
LOCUS       DATXIG010000158          604 bp    DNA     linear   BCT 28-DEC-2023
DEFINITION  TPA_asm: Streptococcus pyogenes strain MGAS11113
            SAMN08555799-rid19682583.denovo.158, whole genome shotgun sequence.
ACCESSION   DATXIG010000158 DATXIG010000000
VERSION     DATXIG010000158.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN08555799
            Sequence Read Archive: SRR6740495
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pyogenes
  ORGANISM  Streptococcus pyogenes
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 604)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 604)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-OCT-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-OCT-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001918058.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001918058.2
            Genome Coverage       :: 368x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/12/2023 11:23:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 1,806
            CDSs (total)                      :: 1,769
            Genes (coding)                    :: 1,722
            CDSs (with protein)               :: 1,722
            Genes (RNA)                       :: 37
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1, 1 (5S, 23S)
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 47
            CDSs (without protein)            :: 47
            Pseudo Genes (ambiguous residues) :: 0 of 47
            Pseudo Genes (frameshifted)       :: 30 of 47
            Pseudo Genes (incomplete)         :: 20 of 47
            Pseudo Genes (internal stop)      :: 9 of 47
            Pseudo Genes (multiple problems)  :: 11 of 47
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..604
                     /organism="Streptococcus pyogenes"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN08555799-rid19682583.denovo.158"
                     /strain="MGAS11113"
                     /serotype="Emm28"
                     /isolation_source="normally sterile site"
                     /host="Homo sapiens"
                     /db_xref="taxon:1314"
                     /country="Finland"
                     /collection_date="2002"
                     /collected_by="National Public Health Laboratory"
     gene            complement(<1..538)
                     /gene="rgg4"
                     /locus_tag="VC122_001779"
     CDS             complement(<1..538)
                     /gene="rgg4"
                     /locus_tag="VC122_001779"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002986681.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator Rgg4/ComR"
                     /protein_id="HEP5641662.1"
                     /translation="MLEHFGGKVKVLRLEKRISREDLCGDESELSVRQLARIELGQSI
                     PSLSKVIFIAKALNVSVGYLTDGADLELPKRYKELKYLILRTPTYMDDGKLQVREEQF
                     DEIFEDYYDKLPEEEKIIIDCLQATLDTLLSENTNFGIDLLQEYFNQIKTKVRFRQND
                     LILLELYLAYLDIEGMDGQ"
BASE COUNT          193 a          124 c           70 g          217 t
ORIGIN      
        1 attgtccatc cattccctca atatcaagat aagctaaata taattctaga agtattaaat
       61 cattttgcct aaagcgtacc ttagtcttta tttgattaaa atattcttga agtaagtcaa
      121 tgccaaagtt agtattctca ctcaataaag tatctaaagt tgcctgtaaa caatcaatga
      181 ttattttctc ttcctctggt aatttatcat aataatcctc aaaaatttca tcaaactgct
      241 cttctcgtac ttgtaatttt ccatcatcca tgtaagttgg tgtccttaag ataaggtatt
      301 ttaattcttt gtaacgctta ggtagttcta aatcagcacc atcagttaag taaccgacac
      361 taacgtttaa ggcttttgca ataaaaataa ccttacttaa acttggtatg gattgaccta
      421 gttctatccg tgctaattga cgaacagaaa gttcagactc atccccacac aagtcctcgc
      481 gactaatcct cttttcaagt cttaacactt ttacttttcc accaaaatgt tctaacatgg
      541 tttctctcca acaactgttt tctttattat atcacagcgt ctaattaaaa actaacattt
      601 ttaa
//
LOCUS       DATXIG010000159          600 bp    DNA     linear   BCT 28-DEC-2023
DEFINITION  TPA_asm: Streptococcus pyogenes strain MGAS11113
            SAMN08555799-rid19682583.denovo.159, whole genome shotgun sequence.
ACCESSION   DATXIG010000159 DATXIG010000000
VERSION     DATXIG010000159.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN08555799
            Sequence Read Archive: SRR6740495
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pyogenes
  ORGANISM  Streptococcus pyogenes
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 600)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 600)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-OCT-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-OCT-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001918058.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001918058.2
            Genome Coverage       :: 368x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/12/2023 11:23:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 1,806
            CDSs (total)                      :: 1,769
            Genes (coding)                    :: 1,722
            CDSs (with protein)               :: 1,722
            Genes (RNA)                       :: 37
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1, 1 (5S, 23S)
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 47
            CDSs (without protein)            :: 47
            Pseudo Genes (ambiguous residues) :: 0 of 47
            Pseudo Genes (frameshifted)       :: 30 of 47
            Pseudo Genes (incomplete)         :: 20 of 47
            Pseudo Genes (internal stop)      :: 9 of 47
            Pseudo Genes (multiple problems)  :: 11 of 47
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..600
                     /organism="Streptococcus pyogenes"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN08555799-rid19682583.denovo.159"
                     /strain="MGAS11113"
                     /serotype="Emm28"
                     /isolation_source="normally sterile site"
                     /host="Homo sapiens"
                     /db_xref="taxon:1314"
                     /country="Finland"
                     /collection_date="2002"
                     /collected_by="National Public Health Laboratory"
     gene            28..543
                     /gene="lepB"
                     /locus_tag="VC122_001780"
     CDS             28..543
                     /gene="lepB"
                     /locus_tag="VC122_001780"
                     /EC_number="3.4.21.89"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014622785.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="signal peptidase I"
                     /protein_id="HEP5641663.1"
                     /translation="MVIIGAILLRIFVFSTFKVSPETANTYLKSGDLVTIKKNIQPKY
                     KDFVVYRVGKKDYVSRVIAVEGDSVTYMDDIFYLNNMVESQAYLEKMKAHYLNHAPFG
                     TLYTDDFTVATITADKYQKVPKGKYLLLNDNRKNTNDSRRFGLINASQIKGLVTFRVL
                     PLSDFGFVEVE"
BASE COUNT          192 a           81 c          114 g          213 t
ORIGIN      
        1 tttataagaa atatattatt gttattaatt gtgattattg gggctatttt gttgagaatc
       61 tttgtttttt caacttttaa agtttctcca gaaacagcta atacttattt aaagagtggt
      121 gatttagtca caatcaaaaa aaatattcag cccaaatata aagattttgt ggtttataga
      181 gttggaaaaa aagattatgt cagtcgagtc attgctgttg aaggcgatag cgtgacttat
      241 atggacgata ttttttacct caataatatg gtagagtcac aggcttacct tgaaaagatg
      301 aaagcacatt acttgaatca tgcaccgttt ggcacattgt atacagatga ttttacagtt
      361 gctaccatca cagctgataa gtatcaaaaa gttcctaagg ggaagtatct tcttttgaat
      421 gataaccgga aaaatacgaa tgacagtcgt cgatttgggt taataaatgc ctcgcagatt
      481 aaaggtttag tgacctttag agttttgcct ctcagcgatt ttggatttgt agaagtagag
      541 tagttgtcag gctactctac tttttttata attagactat accaatttta tgtttgacaa
//
LOCUS       DATXIG010000160          580 bp    DNA     linear   BCT 28-DEC-2023
DEFINITION  TPA_asm: Streptococcus pyogenes strain MGAS11113
            SAMN08555799-rid19682583.denovo.160, whole genome shotgun sequence.
ACCESSION   DATXIG010000160 DATXIG010000000
VERSION     DATXIG010000160.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN08555799
            Sequence Read Archive: SRR6740495
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pyogenes
  ORGANISM  Streptococcus pyogenes
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 580)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 580)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-OCT-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-OCT-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001918058.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001918058.2
            Genome Coverage       :: 368x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/12/2023 11:23:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 1,806
            CDSs (total)                      :: 1,769
            Genes (coding)                    :: 1,722
            CDSs (with protein)               :: 1,722
            Genes (RNA)                       :: 37
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1, 1 (5S, 23S)
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 47
            CDSs (without protein)            :: 47
            Pseudo Genes (ambiguous residues) :: 0 of 47
            Pseudo Genes (frameshifted)       :: 30 of 47
            Pseudo Genes (incomplete)         :: 20 of 47
            Pseudo Genes (internal stop)      :: 9 of 47
            Pseudo Genes (multiple problems)  :: 11 of 47
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..580
                     /organism="Streptococcus pyogenes"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN08555799-rid19682583.denovo.160"
                     /strain="MGAS11113"
                     /serotype="Emm28"
                     /isolation_source="normally sterile site"
                     /host="Homo sapiens"
                     /db_xref="taxon:1314"
                     /country="Finland"
                     /collection_date="2002"
                     /collected_by="National Public Health Laboratory"
     gene            <1..>580
                     /locus_tag="VC122_001781"
     CDS             <1..>580
                     /locus_tag="VC122_001781"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011528611.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="lantibiotic dehydratase"
                     /protein_id="HEP5641664.1"
                     /translation="GANIGSGQAGRSFGRFYGLSETVRETIKNLNHQNNTNVELSFVP
                     KQIRLANVIQNYSDESYNTSFFTTSWDSENELRLEDIYIRYSDGKFHFTTIDGKNELK
                     FTMNNMLNSDSQSRVLRLLVDLSEFEYGLSHWSLFPWDILAQERVYIPEILFEDITIT
                     TAQWNLSAIREEIIHQKIFTGKKDIFNKFRKT"
BASE COUNT          208 a           69 c          114 g          189 t
ORIGIN      
        1 taggagctaa tatcggttct ggacaagcag gtagaagctt tggaagattc tatggattaa
       61 gcgaaacagt aagagaaaca attaagaatt taaaccatca aaataatacg aatgtagagt
      121 tgtcatttgt tcctaaacag ataagacttg cgaatgtaat tcaaaattat agtgatgagt
      181 cttataatac ttcatttttt accacatcat gggattcaga gaacgaactg cgattggagg
      241 atatttatat aagatatagt gatggtaagt ttcattttac aactatcgat gggaaaaatg
      301 agttgaaatt taccatgaat aatatgctaa attccgatag ccaaagcagg gttttgagat
      361 tgttggttga tttatctgag tttgagtacg gtttatcgca ttggtcatta tttccttggg
      421 atattttggc tcaggaaaga gtatatattc ctgaaatttt atttgaagac attacgatta
      481 ccactgctca gtggaattta tcagcaatta gagaagagat tatacatcaa aaaatattta
      541 ctggtaaaaa agatatattc aataagttta gaaagactta
//
LOCUS       DATXIG010000161          579 bp    DNA     linear   BCT 28-DEC-2023
DEFINITION  TPA_asm: Streptococcus pyogenes strain MGAS11113
            SAMN08555799-rid19682583.denovo.161, whole genome shotgun sequence.
ACCESSION   DATXIG010000161 DATXIG010000000
VERSION     DATXIG010000161.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN08555799
            Sequence Read Archive: SRR6740495
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pyogenes
  ORGANISM  Streptococcus pyogenes
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 579)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 579)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-OCT-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-OCT-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001918058.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001918058.2
            Genome Coverage       :: 368x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/12/2023 11:23:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 1,806
            CDSs (total)                      :: 1,769
            Genes (coding)                    :: 1,722
            CDSs (with protein)               :: 1,722
            Genes (RNA)                       :: 37
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1, 1 (5S, 23S)
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 47
            CDSs (without protein)            :: 47
            Pseudo Genes (ambiguous residues) :: 0 of 47
            Pseudo Genes (frameshifted)       :: 30 of 47
            Pseudo Genes (incomplete)         :: 20 of 47
            Pseudo Genes (internal stop)      :: 9 of 47
            Pseudo Genes (multiple problems)  :: 11 of 47
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..579
                     /organism="Streptococcus pyogenes"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN08555799-rid19682583.denovo.161"
                     /strain="MGAS11113"
                     /serotype="Emm28"
                     /isolation_source="normally sterile site"
                     /host="Homo sapiens"
                     /db_xref="taxon:1314"
                     /country="Finland"
                     /collection_date="2002"
                     /collected_by="National Public Health Laboratory"
     repeat_region   191..490
                     /inference="COORDINATES: alignment:crt:1.2"
                     /rpt_family="CRISPR"
                     /rpt_type=direct
                     /rpt_unit_range=257..292
                     /rpt_unit_seq="gttttgggaccattcaaaacagcatagctctaaaac"
BASE COUNT          194 a          115 c           93 g          177 t
ORIGIN      
        1 ttcaactgca caatcatcaa tagaaataat aaaatcaatc atcgtagtca cctcatctat
       61 agtgtactac tttgattgat tttgtcatta tgaatctagt tttctgaaaa caaaagtttc
      121 ttcatgcgtt tggcgtgggt atccgactgc tggtattaac cctctttctc aagttatcat
      181 cggcaatgtt gaatggagac cattcaaaac agcatagctc taaaacggaa agcaggagaa
      241 aggacgttaa tatgtcgttt tgggaccatt caaaacagca tagctctaaa acgtcaaact
      301 caattttttg tctagctcta ttgttttggg accattcaaa acagcatagc tctaaaacag
      361 acaaaagaaa aagcattgtc aatttctagt tttgggacca ttcaaaacag catagctcta
      421 aaacaataat cagtttaaaa atccatctgg agcagttttg ggaccattca aaacagcata
      481 gctctaaaac ctcgtagact atttttgtct aaaaaatttc gtaatcgcac tatttgtctc
      541 agctagactt cagtcttgaa aagcccctgt attactgca
//
LOCUS       DATXIG010000162          578 bp    DNA     linear   BCT 28-DEC-2023
DEFINITION  TPA_asm: Streptococcus pyogenes strain MGAS11113
            SAMN08555799-rid19682583.denovo.162, whole genome shotgun sequence.
ACCESSION   DATXIG010000162 DATXIG010000000
VERSION     DATXIG010000162.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN08555799
            Sequence Read Archive: SRR6740495
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pyogenes
  ORGANISM  Streptococcus pyogenes
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 578)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 578)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-OCT-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-OCT-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001918058.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001918058.2
            Genome Coverage       :: 368x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/12/2023 11:23:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 1,806
            CDSs (total)                      :: 1,769
            Genes (coding)                    :: 1,722
            CDSs (with protein)               :: 1,722
            Genes (RNA)                       :: 37
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1, 1 (5S, 23S)
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 47
            CDSs (without protein)            :: 47
            Pseudo Genes (ambiguous residues) :: 0 of 47
            Pseudo Genes (frameshifted)       :: 30 of 47
            Pseudo Genes (incomplete)         :: 20 of 47
            Pseudo Genes (internal stop)      :: 9 of 47
            Pseudo Genes (multiple problems)  :: 11 of 47
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..578
                     /organism="Streptococcus pyogenes"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN08555799-rid19682583.denovo.162"
                     /strain="MGAS11113"
                     /serotype="Emm28"
                     /isolation_source="normally sterile site"
                     /host="Homo sapiens"
                     /db_xref="taxon:1314"
                     /country="Finland"
                     /collection_date="2002"
                     /collected_by="National Public Health Laboratory"
     gene            374..535
                     /locus_tag="VC122_001782"
     CDS             374..535
                     /locus_tag="VC122_001782"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005761276.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEP5641665.1"
                     /translation="MDNDWNGLADLIANLIAKYAGALDLDNLPDPTPAKNQEMKNSFD
                     KAKTQIETD"
BASE COUNT          229 a           82 c          115 g          152 t
ORIGIN      
        1 aagattaata actctcgata aattttgaac cttaacaatt gaatagacca agacaagacg
       61 ttaattattt ttggagcgta ataacctatc cgtcaagatc tttctgctga ataattcggc
      121 aaaacaagta ctgctaagca aaaaaaggaa gaagaaagag agcgataagt aagagtttga
      181 atatagggag ggatacccta tccgctatga agaaaatgag gataatgata cttctaaggt
      241 tgaagccggc tcatccggaa tagaggcgga ggtgagattc ctatgttgct aatccaaagc
      301 acttgtcaat gtcgtaaaac ttaattaaac aaataattat tagatataaa actttataga
      361 gaggaggtat atattggaca atgactggaa tggattggct gatttaatag caaatctgat
      421 tgcaaaatat gctggtgctt tagatttaga taatctgccc gatccaacgc cagcaaaaaa
      481 tcaagagatg aaaaatagtt ttgacaaggc aaaaacacaa attgagacgg actaataaaa
      541 gtgttataat atacttgaaa tgatagtcca aacttaaa
//
LOCUS       DATXIG010000163          573 bp    DNA     linear   BCT 28-DEC-2023
DEFINITION  TPA_asm: Streptococcus pyogenes strain MGAS11113
            SAMN08555799-rid19682583.denovo.163, whole genome shotgun sequence.
ACCESSION   DATXIG010000163 DATXIG010000000
VERSION     DATXIG010000163.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN08555799
            Sequence Read Archive: SRR6740495
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pyogenes
  ORGANISM  Streptococcus pyogenes
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 573)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 573)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-OCT-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-OCT-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001918058.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001918058.2
            Genome Coverage       :: 368x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/12/2023 11:23:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 1,806
            CDSs (total)                      :: 1,769
            Genes (coding)                    :: 1,722
            CDSs (with protein)               :: 1,722
            Genes (RNA)                       :: 37
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1, 1 (5S, 23S)
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 47
            CDSs (without protein)            :: 47
            Pseudo Genes (ambiguous residues) :: 0 of 47
            Pseudo Genes (frameshifted)       :: 30 of 47
            Pseudo Genes (incomplete)         :: 20 of 47
            Pseudo Genes (internal stop)      :: 9 of 47
            Pseudo Genes (multiple problems)  :: 11 of 47
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..573
                     /organism="Streptococcus pyogenes"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN08555799-rid19682583.denovo.163"
                     /strain="MGAS11113"
                     /serotype="Emm28"
                     /isolation_source="normally sterile site"
                     /host="Homo sapiens"
                     /db_xref="taxon:1314"
                     /country="Finland"
                     /collection_date="2002"
                     /collected_by="National Public Health Laboratory"
     gene            complement(<1..>573)
                     /locus_tag="VC122_001783"
     CDS             complement(<1..>573)
                     /locus_tag="VC122_001783"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010922705.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="exotoxin"
                     /protein_id="HEP5641666.1"
                     /translation="IFSFTSIFIAIISRPVFGLEVDNNSLLRNIYSTIVYEYSDTVID
                     FKTSHNLVTKKLDVRDARDFFINSEMDEYAANDFKAGDRIAVFSVPFDWNYLSKGKVT
                     AYTYGGITPYQKTSIPKNIPVNLWINRKQISVPYNEISTNKTTVTAQEIDLKVRKFLI
                     SQHQLYSSGSSYKSGKLVFHTNDNSDKYSL"
BASE COUNT          191 a           82 c           91 g          209 t
ORIGIN      
        1 tcgagagaat atttatctga attatcattt gtatgaaaaa ctaatttacc acttttgtag
       61 ctagaaccag aagaatataa ttgatgttgt gatattaaaa attttctaac ctttagatca
      121 atttcttgag ctgtaactgt tgttttgtta gttgatattt cgttgtaagg aacagagatc
      181 tgctttctat taatccataa attaacaggg atatttttag gtattgaagt tttttggtag
      241 ggtgttattc cgccgtacgt atatgctgtg acttttcctt ttgataaata gttccaatca
      301 aatgggacgg agaacacagc tattctatct ccagctttaa aatcattggc tgcatattcg
      361 tccatttcgg agttaataaa aaaatctcta gcatctctaa catcaagttt cttagtcact
      421 aagttatgac tggttttaaa atcaattact gtatctgaat attcatatac aatcgtacta
      481 tagatattcc ttagaaggga attattatct acttctaatc caaacacagg acgagaaatt
      541 attgcaatga atattgaagt aaaagaaaaa ata
//
LOCUS       DATXIG010000164          538 bp    DNA     linear   BCT 28-DEC-2023
DEFINITION  TPA_asm: Streptococcus pyogenes strain MGAS11113
            SAMN08555799-rid19682583.denovo.164, whole genome shotgun sequence.
ACCESSION   DATXIG010000164 DATXIG010000000
VERSION     DATXIG010000164.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN08555799
            Sequence Read Archive: SRR6740495
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pyogenes
  ORGANISM  Streptococcus pyogenes
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 538)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 538)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-OCT-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-OCT-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001918058.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001918058.2
            Genome Coverage       :: 368x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/12/2023 11:23:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 1,806
            CDSs (total)                      :: 1,769
            Genes (coding)                    :: 1,722
            CDSs (with protein)               :: 1,722
            Genes (RNA)                       :: 37
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1, 1 (5S, 23S)
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 47
            CDSs (without protein)            :: 47
            Pseudo Genes (ambiguous residues) :: 0 of 47
            Pseudo Genes (frameshifted)       :: 30 of 47
            Pseudo Genes (incomplete)         :: 20 of 47
            Pseudo Genes (internal stop)      :: 9 of 47
            Pseudo Genes (multiple problems)  :: 11 of 47
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..538
                     /organism="Streptococcus pyogenes"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN08555799-rid19682583.denovo.164"
                     /strain="MGAS11113"
                     /serotype="Emm28"
                     /isolation_source="normally sterile site"
                     /host="Homo sapiens"
                     /db_xref="taxon:1314"
                     /country="Finland"
                     /collection_date="2002"
                     /collected_by="National Public Health Laboratory"
     gene            complement(<1..>538)
                     /locus_tag="VC122_001784"
     CDS             complement(<1..>538)
                     /locus_tag="VC122_001784"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011528611.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lantibiotic dehydratase"
                     /protein_id="HEP5641667.1"
                     /translation="KLEEGTLCTDLTTLKKMIEDYNDLNIGEGRDLLLQIFDKMESIY
                     KYDNGGNYLKVDSLAEYEGGFNILSETARESIERLAEMFVKLNTNVKRRGGRKNSLEE
                     YQLKFIGKYGENCSIPFVEVINKDAGIGFPEYYKGGEGEAIELSDPIMQMFEKKYEEA
                     LLNGGHIEFYSKDLDDFQT"
BASE COUNT          154 a          119 c           52 g          213 t
ORIGIN      
        1 ctgtttgaaa atcatctaaa tcttttgaat aaaattcgat atgccctcca tttaatagag
       61 cttcctcata tttcttttca aacatttgca ttattggatc gcttaactct attgcttcac
      121 cttctcctcc tttgtaatat tcaggaaaac ctatgcctgc atctttattt attacttcaa
      181 caaaaggaat tgagcagttt tctccatatt tgccaataaa tttcaactga tactcttcaa
      241 gtgaattttt cctcccccct cttctcttta catttgtatt taattttaca aacatctctg
      301 cgagtcgttc tatactttcc cgagccgttt cagatagaat attaaaaccg ccttcatact
      361 ctgctaatga atcaactttt aagtaatttc caccgttatc atatttataa atactttcca
      421 ttttatcaaa aatctgcaaa agtaagtctc taccttcccc aatatttaaa tcgttgtaat
      481 cttcaatcat cttctttaaa gtagttaagt cagtacatag tgttccttct tctaattt
//
LOCUS       DATXIG010000165          535 bp    DNA     linear   BCT 28-DEC-2023
DEFINITION  TPA_asm: Streptococcus pyogenes strain MGAS11113
            SAMN08555799-rid19682583.denovo.165, whole genome shotgun sequence.
ACCESSION   DATXIG010000165 DATXIG010000000
VERSION     DATXIG010000165.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN08555799
            Sequence Read Archive: SRR6740495
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pyogenes
  ORGANISM  Streptococcus pyogenes
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 535)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 535)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-OCT-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-OCT-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001918058.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001918058.2
            Genome Coverage       :: 368x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/12/2023 11:23:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 1,806
            CDSs (total)                      :: 1,769
            Genes (coding)                    :: 1,722
            CDSs (with protein)               :: 1,722
            Genes (RNA)                       :: 37
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1, 1 (5S, 23S)
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 47
            CDSs (without protein)            :: 47
            Pseudo Genes (ambiguous residues) :: 0 of 47
            Pseudo Genes (frameshifted)       :: 30 of 47
            Pseudo Genes (incomplete)         :: 20 of 47
            Pseudo Genes (internal stop)      :: 9 of 47
            Pseudo Genes (multiple problems)  :: 11 of 47
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..535
                     /organism="Streptococcus pyogenes"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN08555799-rid19682583.denovo.165"
                     /strain="MGAS11113"
                     /serotype="Emm28"
                     /isolation_source="normally sterile site"
                     /host="Homo sapiens"
                     /db_xref="taxon:1314"
                     /country="Finland"
                     /collection_date="2002"
                     /collected_by="National Public Health Laboratory"
     gene            257..>535
                     /locus_tag="VC122_001785"
     CDS             257..>535
                     /locus_tag="VC122_001785"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001188032.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF771 domain-containing protein"
                     /protein_id="HEP5641668.1"
                     /translation="MQQVFNVSVPVPDDVVIISKEEYLDLLSDNEQGKWWDIDNLQEL
                     LGLGRSKLINDILLNPDIKKEVDLSINPNGFIVYPRGKGSRYKILATRA"
BASE COUNT          191 a           46 c          109 g          189 t
ORIGIN      
        1 ttgcatgatt ctaaaaaaag agaagttaga aagtatttgt tatttttgat aaatgatatt
       61 ttattagata tggagaaacg ttgatgtcat gattagtttc atggcttttt tgtttgtttt
      121 ttattaaaga gcaaagttcg gggatactgt atataaatta aagctaaaga tgaaaaatag
      181 ttcgatttta aattagagaa cctgcttaac cggaacaaat aatgttcgga gtaattaaat
      241 tataaggaga atgagtatgc aacaagtttt taatgtgagt gttcctgtac cagatgatgt
      301 tgttatcatt tctaaagagg aatatttaga tttgttaagt gataatgaac aaggaaaatg
      361 gtgggatatt gataatttgc aagaattgtt gggccttggt aggtcaaagt taattaatga
      421 tatcttatta aacccagata ttaaaaagga agttgattta tcaatcaatc caaatggttt
      481 tattgtttat cctaggggga aaggtagccg ttataaaata ttagcaacta gagct
//
LOCUS       DATXIG010000166          519 bp    DNA     linear   BCT 28-DEC-2023
DEFINITION  TPA_asm: Streptococcus pyogenes strain MGAS11113
            SAMN08555799-rid19682583.denovo.166, whole genome shotgun sequence.
ACCESSION   DATXIG010000166 DATXIG010000000
VERSION     DATXIG010000166.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN08555799
            Sequence Read Archive: SRR6740495
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pyogenes
  ORGANISM  Streptococcus pyogenes
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 519)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 519)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-OCT-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-OCT-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001918058.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001918058.2
            Genome Coverage       :: 368x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/12/2023 11:23:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 1,806
            CDSs (total)                      :: 1,769
            Genes (coding)                    :: 1,722
            CDSs (with protein)               :: 1,722
            Genes (RNA)                       :: 37
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1, 1 (5S, 23S)
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 47
            CDSs (without protein)            :: 47
            Pseudo Genes (ambiguous residues) :: 0 of 47
            Pseudo Genes (frameshifted)       :: 30 of 47
            Pseudo Genes (incomplete)         :: 20 of 47
            Pseudo Genes (internal stop)      :: 9 of 47
            Pseudo Genes (multiple problems)  :: 11 of 47
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..519
                     /organism="Streptococcus pyogenes"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN08555799-rid19682583.denovo.166"
                     /strain="MGAS11113"
                     /serotype="Emm28"
                     /isolation_source="normally sterile site"
                     /host="Homo sapiens"
                     /db_xref="taxon:1314"
                     /country="Finland"
                     /collection_date="2002"
                     /collected_by="National Public Health Laboratory"
     gene            151..>519
                     /locus_tag="VC122_001786"
     CDS             151..>519
                     /locus_tag="VC122_001786"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000619317.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEP5641669.1"
                     /translation="MIRIKNITKSKFFGTAIILLQQLIALLILVYNRENLSLLFSEKV
                     ALVMTLIDTAFIWLATILRQKQGDIFKRIISIISLTIWQYLVSVLTRGTPLFLSSGLQ
                     IILLYCYTVEITNLILYGHKQ"
BASE COUNT          171 a           80 c           76 g          192 t
ORIGIN      
        1 ggcaatatca taatgttatc tttattgctt tgtctgcttt tcttcttgat aatatatggc
       61 acattttatg tattgacatt gcggtcagtg acatccataa tcaaaaatta attttaaaaa
      121 tcttttaact ttaacgatta agtaaataat atgataagaa taaaaaacat aacaaagagt
      181 aagttctttg ggactgcaat aatcttgttg caacaattaa tagccttgtt aattttggtc
      241 tataatcgag agaacctgtc tcttcttttc tctgaaaaag tagctcttgt aatgaccctt
      301 attgatacag cttttatttg gctggcaaca atattacgcc aaaaacaagg tgatattttt
      361 aaaaggatta tttctatcat ctccttaaca atttggcaat acttagtatc tgtgctgaca
      421 agaggaactc ctctcttttt aagcagtgga ctacagatta tattgctcta ttgttatacc
      481 gttgagatta ctaatttaat attatatggg cacaaacaa
//
LOCUS       DATXIG010000167          511 bp    DNA     linear   BCT 28-DEC-2023
DEFINITION  TPA_asm: Streptococcus pyogenes strain MGAS11113
            SAMN08555799-rid19682583.denovo.167, whole genome shotgun sequence.
ACCESSION   DATXIG010000167 DATXIG010000000
VERSION     DATXIG010000167.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN08555799
            Sequence Read Archive: SRR6740495
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pyogenes
  ORGANISM  Streptococcus pyogenes
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 511)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 511)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-OCT-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-OCT-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001918058.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001918058.2
            Genome Coverage       :: 368x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/12/2023 11:23:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 1,806
            CDSs (total)                      :: 1,769
            Genes (coding)                    :: 1,722
            CDSs (with protein)               :: 1,722
            Genes (RNA)                       :: 37
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1, 1 (5S, 23S)
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 47
            CDSs (without protein)            :: 47
            Pseudo Genes (ambiguous residues) :: 0 of 47
            Pseudo Genes (frameshifted)       :: 30 of 47
            Pseudo Genes (incomplete)         :: 20 of 47
            Pseudo Genes (internal stop)      :: 9 of 47
            Pseudo Genes (multiple problems)  :: 11 of 47
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..511
                     /organism="Streptococcus pyogenes"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN08555799-rid19682583.denovo.167"
                     /strain="MGAS11113"
                     /serotype="Emm28"
                     /isolation_source="normally sterile site"
                     /host="Homo sapiens"
                     /db_xref="taxon:1314"
                     /country="Finland"
                     /collection_date="2002"
                     /collected_by="National Public Health Laboratory"
     gene            <1..>511
                     /locus_tag="VC122_001787"
     CDS             <1..>511
                     /locus_tag="VC122_001787"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000564846.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="ISAs1-like element IS1548 family transposase"
                     /protein_id="HEP5641670.1"
                     /translation="VQFEQSLTSLDAVHQLISVDGKTIRGNRGKNQKPVHIVTAYDGG
                     HHLSLGQVAVEEKSNEIVAIPQLLRTIDIRKSIVTIDAMGTQTAIVDTIIKGKADYCL
                     AVKGNQETLYDDIALYFSDVNLLEELQENAQYYQTVEKSRGQIEVREYWVSSDIKWLC
                     QNHPKWHKLR"
BASE COUNT          169 a           82 c          118 g          142 t
ORIGIN      
        1 aagttcaatt tgagcaatca ttgacaagct tagatgccgt tcatcaactg atttcagtgg
       61 acggtaaaac gattcgaggc aatcgaggta aaaatcagaa gcctgttcat attgtaacgg
      121 cttatgatgg gggtcatcat cttagtttgg gacaggtagc ggttgaggag aaaagtaatg
      181 aaattgttgc cattcctcag ttattgcgga caattgatat ccgtaaaagc attgtaacga
      241 tagacgcaat gggcacgcag acggctatcg ttgatacgat tataaaaggt aaagcagact
      301 attgcttagc cgtcaaagga aatcaagaaa cactttatga tgatattgct ctttatttta
      361 gtgatgtcaa cttattggaa gaactccaag aaaatgcaca gtattatcag actgttgaaa
      421 aatctagggg acagattgaa gttagagaat actgggtgtc ttccgatatc aaatggttgt
      481 gtcaaaacca tcccaaatgg cataagttac g
//
LOCUS       DATXIG010000168          495 bp    DNA     linear   BCT 28-DEC-2023
DEFINITION  TPA_asm: Streptococcus pyogenes strain MGAS11113
            SAMN08555799-rid19682583.denovo.168, whole genome shotgun sequence.
ACCESSION   DATXIG010000168 DATXIG010000000
VERSION     DATXIG010000168.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN08555799
            Sequence Read Archive: SRR6740495
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pyogenes
  ORGANISM  Streptococcus pyogenes
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 495)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 495)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-OCT-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-OCT-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001918058.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001918058.2
            Genome Coverage       :: 368x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/12/2023 11:23:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 1,806
            CDSs (total)                      :: 1,769
            Genes (coding)                    :: 1,722
            CDSs (with protein)               :: 1,722
            Genes (RNA)                       :: 37
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1, 1 (5S, 23S)
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 47
            CDSs (without protein)            :: 47
            Pseudo Genes (ambiguous residues) :: 0 of 47
            Pseudo Genes (frameshifted)       :: 30 of 47
            Pseudo Genes (incomplete)         :: 20 of 47
            Pseudo Genes (internal stop)      :: 9 of 47
            Pseudo Genes (multiple problems)  :: 11 of 47
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..495
                     /organism="Streptococcus pyogenes"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN08555799-rid19682583.denovo.168"
                     /strain="MGAS11113"
                     /serotype="Emm28"
                     /isolation_source="normally sterile site"
                     /host="Homo sapiens"
                     /db_xref="taxon:1314"
                     /country="Finland"
                     /collection_date="2002"
                     /collected_by="National Public Health Laboratory"
     gene            <1..>495
                     /locus_tag="VC122_001788"
     CDS             <1..>495
                     /locus_tag="VC122_001788"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011528611.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="thiopeptide-type bacteriocin biosynthesis
                     protein"
                     /protein_id="HEP5641671.1"
                     /translation="SLRGIEQGESIFKDNLGNYNTEIVVPILKESNGINNTNDNVQFK
                     NEVKKIYLPFCDWLFFKFYGPKERQEELLNNWKKFWLNLPSELFSQSNMFYMRYNDTN
                     DHIRIRINCDSIENNFSLYLSVVQDLLPQLIESCIISDIEVSSYKPEVNRYGGPHLIS
                     YAEE"
BASE COUNT          190 a           59 c           88 g          158 t
ORIGIN      
        1 ttctttgaga ggaattgagc agggagagtc tatttttaag gataatttag ggaactacaa
       61 tacagagata gtagttccaa ttcttaagga atctaatgga ataaacaata caaacgataa
      121 tgttcaattt aaaaatgaag ttaagaaaat atatttacca ttttgtgact ggctattttt
      181 caaattttat ggtccaaaag agcgacaaga agaattacta aataactgga aaaaattttg
      241 gctaaacctg ccatcagaac tgtttagcca aagtaacatg ttttatatga gatataatga
      301 taccaatgat catataagaa ttcgaataaa ttgtgattcg attgaaaata acttttcttt
      361 gtatttgtca gtagttcaag atctattacc tcaactgatt gagagttgta ttatctctga
      421 catagaagtg agtagctata aaccagaagt aaatagatat ggaggacctc atttaataag
      481 ttatgcagag gaaat
//
LOCUS       DATXIG010000169          477 bp    DNA     linear   BCT 28-DEC-2023
DEFINITION  TPA_asm: Streptococcus pyogenes strain MGAS11113
            SAMN08555799-rid19682583.denovo.169, whole genome shotgun sequence.
ACCESSION   DATXIG010000169 DATXIG010000000
VERSION     DATXIG010000169.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN08555799
            Sequence Read Archive: SRR6740495
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pyogenes
  ORGANISM  Streptococcus pyogenes
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 477)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 477)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-OCT-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-OCT-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001918058.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001918058.2
            Genome Coverage       :: 368x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/12/2023 11:23:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 1,806
            CDSs (total)                      :: 1,769
            Genes (coding)                    :: 1,722
            CDSs (with protein)               :: 1,722
            Genes (RNA)                       :: 37
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1, 1 (5S, 23S)
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 47
            CDSs (without protein)            :: 47
            Pseudo Genes (ambiguous residues) :: 0 of 47
            Pseudo Genes (frameshifted)       :: 30 of 47
            Pseudo Genes (incomplete)         :: 20 of 47
            Pseudo Genes (internal stop)      :: 9 of 47
            Pseudo Genes (multiple problems)  :: 11 of 47
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..477
                     /organism="Streptococcus pyogenes"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN08555799-rid19682583.denovo.169"
                     /strain="MGAS11113"
                     /serotype="Emm28"
                     /isolation_source="normally sterile site"
                     /host="Homo sapiens"
                     /db_xref="taxon:1314"
                     /country="Finland"
                     /collection_date="2002"
                     /collected_by="National Public Health Laboratory"
     gene            <1..169
                     /locus_tag="VC122_001789"
     CDS             <1..169
                     /locus_tag="VC122_001789"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007516553.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="DUF3847 domain-containing protein"
                     /protein_id="HEP5641672.1"
                     /translation="KRRKERTHRLITRGAILESLIENAEELTDEEIKILLEEAIKTKE
                     FKETLKLMREN"
BASE COUNT          184 a           96 c           92 g          105 t
ORIGIN      
        1 aaaaagaaga aaagaaagaa cacataggct cataacaaga ggagcaatct tagaaagcct
       61 tatagaaaat gcagaagaac taacagatga agaaataaaa atcctactag aagaagcaat
      121 aaagacaaaa gaatttaaag aaacactaaa actaatgaga gaaaattaat aaaaataaag
      181 ggtgttgtaa ataatcaaat ctcccttaga ttttctaagg gcgcaattat acagctatca
      241 gagattgatg cattgcgttc tccgaaggct cctaatcgga gggctgtgat ttgtgcgatc
      301 aatgagccaa acggatatac ttgcatattc actcgccgaa tgccccaaca gccgacagaa
      361 tgtcggcttc cgcagtcatg gtctgctcca aatcaaacac ggacgctacg cttcccctgc
      421 gctggctgcc gccagccacc ctttgaaagg aaaaattttt ataaacagta tttacta
//
LOCUS       DATXIG010000170          471 bp    DNA     linear   BCT 28-DEC-2023
DEFINITION  TPA_asm: Streptococcus pyogenes strain MGAS11113
            SAMN08555799-rid19682583.denovo.170, whole genome shotgun sequence.
ACCESSION   DATXIG010000170 DATXIG010000000
VERSION     DATXIG010000170.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN08555799
            Sequence Read Archive: SRR6740495
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pyogenes
  ORGANISM  Streptococcus pyogenes
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 471)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 471)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-OCT-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-OCT-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001918058.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001918058.2
            Genome Coverage       :: 368x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/12/2023 11:23:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 1,806
            CDSs (total)                      :: 1,769
            Genes (coding)                    :: 1,722
            CDSs (with protein)               :: 1,722
            Genes (RNA)                       :: 37
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1, 1 (5S, 23S)
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 47
            CDSs (without protein)            :: 47
            Pseudo Genes (ambiguous residues) :: 0 of 47
            Pseudo Genes (frameshifted)       :: 30 of 47
            Pseudo Genes (incomplete)         :: 20 of 47
            Pseudo Genes (internal stop)      :: 9 of 47
            Pseudo Genes (multiple problems)  :: 11 of 47
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..471
                     /organism="Streptococcus pyogenes"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN08555799-rid19682583.denovo.170"
                     /strain="MGAS11113"
                     /serotype="Emm28"
                     /isolation_source="normally sterile site"
                     /host="Homo sapiens"
                     /db_xref="taxon:1314"
                     /country="Finland"
                     /collection_date="2002"
                     /collected_by="National Public Health Laboratory"
     gene            <1..413
                     /locus_tag="VC122_001790"
     CDS             <1..413
                     /locus_tag="VC122_001790"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012560530.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="S-adenosylmethionine synthetase"
                     /protein_id="HEP5641673.1"
                     /translation="HYYLTTTGSCIDFGEEGAVGRGNKTHGIISSFRPNTMEAPAGKN
                     CTYFVGKVWGFLSDTIAKEIYEAFNTPCQIIMQLNIGSKLYRPTHLFIQTEESVDQER
                     VLEIVNRHLNNGKQNTNLILSTQHFIPKTNVYDG"
BASE COUNT          162 a           61 c           81 g          167 t
ORIGIN      
        1 accattatta tttaactact acaggttctt gtattgattt cggagaagaa ggtgcagttg
       61 gtcgaggtaa taaaactcat ggtatcatat caagttttag gccaaatact atggaagcac
      121 cagctggaaa aaattgtact tattttgtag gaaaggtttg gggatttttg tctgatacta
      181 ttgccaaaga aatttatgaa gcattcaata ctccctgtca aatcattatg caattaaata
      241 ttggatcaaa attgtataga ccaacccatt tatttattca gacagaagaa agtgttgatc
      301 aagaaagagt attagaaatt gttaacaggc atttaaataa tggtaaacaa aatactaatt
      361 taatcttgtc aactcaacat tttattccta aaaccaatgt ttatgatgga taaaactgtt
      421 ttatattttg ggttggttga aatttctttt gatgtgcctg actttatatt t
//
LOCUS       DATXIG010000171          452 bp    DNA     linear   BCT 28-DEC-2023
DEFINITION  TPA_asm: Streptococcus pyogenes strain MGAS11113
            SAMN08555799-rid19682583.denovo.171, whole genome shotgun sequence.
ACCESSION   DATXIG010000171 DATXIG010000000
VERSION     DATXIG010000171.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN08555799
            Sequence Read Archive: SRR6740495
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pyogenes
  ORGANISM  Streptococcus pyogenes
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 452)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 452)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-OCT-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-OCT-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001918058.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001918058.2
            Genome Coverage       :: 368x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/12/2023 11:23:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 1,806
            CDSs (total)                      :: 1,769
            Genes (coding)                    :: 1,722
            CDSs (with protein)               :: 1,722
            Genes (RNA)                       :: 37
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1, 1 (5S, 23S)
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 47
            CDSs (without protein)            :: 47
            Pseudo Genes (ambiguous residues) :: 0 of 47
            Pseudo Genes (frameshifted)       :: 30 of 47
            Pseudo Genes (incomplete)         :: 20 of 47
            Pseudo Genes (internal stop)      :: 9 of 47
            Pseudo Genes (multiple problems)  :: 11 of 47
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..452
                     /organism="Streptococcus pyogenes"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN08555799-rid19682583.denovo.171"
                     /strain="MGAS11113"
                     /serotype="Emm28"
                     /isolation_source="normally sterile site"
                     /host="Homo sapiens"
                     /db_xref="taxon:1314"
                     /country="Finland"
                     /collection_date="2002"
                     /collected_by="National Public Health Laboratory"
     gene            complement(<1..>452)
                     /locus_tag="VC122_001791"
     CDS             complement(<1..>452)
                     /locus_tag="VC122_001791"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009880288.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="glycosyl transferase 2 family protein"
                     /protein_id="HEP5641674.1"
                     /translation="TDGTPQIFLETKTYNTKVSIVSEAKGKGYNVRNIFEYAINHVPN
                     FSGLILIDGDVVSMKKMWLEKMFIAIESGNDLIIPNYARKSFEGNATNHFIYPMLVKF
                     FKRDMPYQCISGDFGFSRGLIKDLTLKCNWHKYTLGYGIDIFLTLTAI"
BASE COUNT          154 a           80 c           57 g          161 t
ORIGIN      
        1 atatagctgt taaggttaaa aaaatatcta tcccgtaacc taatgtatat ttgtgccaat
       61 tacacttcaa agttaaatct tttatcaatc ctcttgaaaa tccaaaatca ccagagatgc
      121 attgataagg catatcacgt ttgaaaaatt tcactaacat gggataaata aaatggtttg
      181 tcgcatttcc ttcaaaagat tttctagcat aatttgggat tataagatca tttccagatt
      241 caattgctat aaacatcttt tctaaccaca tttttttcat tgagacaacg tctccatcaa
      301 tcaaaattaa accggagaaa ttcggcacat gatttattgc atattcgaaa atatttctca
      361 cattataacc ttttccttta gcttctgata ctatcgaaac tttggtgtta taagttttag
      421 tttctaaaaa aatctgcgga gttccatctg ta
//
LOCUS       DATXIG010000172          449 bp    DNA     linear   BCT 28-DEC-2023
DEFINITION  TPA_asm: Streptococcus pyogenes strain MGAS11113
            SAMN08555799-rid19682583.denovo.172, whole genome shotgun sequence.
ACCESSION   DATXIG010000172 DATXIG010000000
VERSION     DATXIG010000172.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN08555799
            Sequence Read Archive: SRR6740495
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pyogenes
  ORGANISM  Streptococcus pyogenes
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 449)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 449)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-OCT-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-OCT-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001918058.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001918058.2
            Genome Coverage       :: 368x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/12/2023 11:23:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 1,806
            CDSs (total)                      :: 1,769
            Genes (coding)                    :: 1,722
            CDSs (with protein)               :: 1,722
            Genes (RNA)                       :: 37
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1, 1 (5S, 23S)
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 47
            CDSs (without protein)            :: 47
            Pseudo Genes (ambiguous residues) :: 0 of 47
            Pseudo Genes (frameshifted)       :: 30 of 47
            Pseudo Genes (incomplete)         :: 20 of 47
            Pseudo Genes (internal stop)      :: 9 of 47
            Pseudo Genes (multiple problems)  :: 11 of 47
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..449
                     /organism="Streptococcus pyogenes"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN08555799-rid19682583.denovo.172"
                     /strain="MGAS11113"
                     /serotype="Emm28"
                     /isolation_source="normally sterile site"
                     /host="Homo sapiens"
                     /db_xref="taxon:1314"
                     /country="Finland"
                     /collection_date="2002"
                     /collected_by="National Public Health Laboratory"
     gene            210..425
                     /locus_tag="VC122_001792"
                     /pseudo
     CDS             210..425
                     /locus_tag="VC122_001792"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611513.1"
                     /note="internal stop; incomplete; partial in the middle of
                     a contig; missing N-terminus and C-terminus; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
BASE COUNT          172 a           75 c           74 g          128 t
ORIGIN      
        1 tggactaaat aagctaaaag ctctggataa atatgatcaa ttgacctata ataacattga
       61 taccaaatct aaaactgctc agattgaatt gcgccccaaa agttagacaa aaaatctaat
      121 aattggggtg tttttgtctg gcattaagtt tagctaataa ccttgcttaa atcacattaa
      181 accaataatg gaaaaaggca tctccaaata aatgctatga cggaactata tgatagagtg
      241 atcaaggttg gcaatatcaa cacaactatt atcatcattt cctaaactct aaaggaattc
      301 gtccgtctat gtcacgtaaa agaaacaggc ctgacaatgg catgatggaa tctttctttg
      361 gcatcttaaa atcagaaatg ttctatggtt atgagaataa ctgtaaatca ctagaagact
      421 cagaataagc gattacaaat gacattgat
//
LOCUS       DATXIG010000173          420 bp    DNA     linear   BCT 28-DEC-2023
DEFINITION  TPA_asm: Streptococcus pyogenes strain MGAS11113
            SAMN08555799-rid19682583.denovo.173, whole genome shotgun sequence.
ACCESSION   DATXIG010000173 DATXIG010000000
VERSION     DATXIG010000173.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN08555799
            Sequence Read Archive: SRR6740495
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pyogenes
  ORGANISM  Streptococcus pyogenes
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 420)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 420)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-OCT-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-OCT-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001918058.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001918058.2
            Genome Coverage       :: 368x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/12/2023 11:23:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 1,806
            CDSs (total)                      :: 1,769
            Genes (coding)                    :: 1,722
            CDSs (with protein)               :: 1,722
            Genes (RNA)                       :: 37
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1, 1 (5S, 23S)
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 47
            CDSs (without protein)            :: 47
            Pseudo Genes (ambiguous residues) :: 0 of 47
            Pseudo Genes (frameshifted)       :: 30 of 47
            Pseudo Genes (incomplete)         :: 20 of 47
            Pseudo Genes (internal stop)      :: 9 of 47
            Pseudo Genes (multiple problems)  :: 11 of 47
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..420
                     /organism="Streptococcus pyogenes"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN08555799-rid19682583.denovo.173"
                     /strain="MGAS11113"
                     /serotype="Emm28"
                     /isolation_source="normally sterile site"
                     /host="Homo sapiens"
                     /db_xref="taxon:1314"
                     /country="Finland"
                     /collection_date="2002"
                     /collected_by="National Public Health Laboratory"
     gene            complement(73..>420)
                     /locus_tag="VC122_001793"
     CDS             complement(73..>420)
                     /locus_tag="VC122_001793"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011106857.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="shikimate dehydrogenase"
                     /protein_id="HEP5641675.1"
                     /translation="ADQQILNDIEIIINTTPVGMINYEESLPINFDHLDNVNYKDKYF
                     MDVVFNPLHTKLHEYFGKRGAKIIDGLWVLIFQGIEAFSLWSDKINGGKYFFSKDDVV
                     LIHTFLEGKIKGK"
BASE COUNT          151 a           69 c           38 g          162 t
ORIGIN      
        1 atctatttaa aaatttcaat gaatcatcta gtttgtctga tatttcatca agatttactg
       61 taatatgaaa agtcattttc cttttatctt cccttctaga aatgtatgaa ttaggacaac
      121 atcatctttt gaaaaaaaat atttaccacc attaatttta tcactccaca atgaaaatgc
      181 ttcaattccc tgaaaaatca aaacccataa accatcaatt atttttgctc ctcttttacc
      241 aaaatattca tgaagtttcg tatgtaaagg attgaaaact acatccataa agtatttatc
      301 tttataatta acattgtcca aatgatcaaa atttataggt aaactttctt cataatttat
      361 cataccaact ggtgtagtat ttattatgat ttctatatca tttaaaattt gttgatcagc
//
LOCUS       DATXIG010000174          415 bp    DNA     linear   BCT 28-DEC-2023
DEFINITION  TPA_asm: Streptococcus pyogenes strain MGAS11113
            SAMN08555799-rid19682583.denovo.174, whole genome shotgun sequence.
ACCESSION   DATXIG010000174 DATXIG010000000
VERSION     DATXIG010000174.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN08555799
            Sequence Read Archive: SRR6740495
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pyogenes
  ORGANISM  Streptococcus pyogenes
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 415)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 415)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-OCT-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-OCT-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001918058.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001918058.2
            Genome Coverage       :: 368x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/12/2023 11:23:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 1,806
            CDSs (total)                      :: 1,769
            Genes (coding)                    :: 1,722
            CDSs (with protein)               :: 1,722
            Genes (RNA)                       :: 37
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1, 1 (5S, 23S)
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 47
            CDSs (without protein)            :: 47
            Pseudo Genes (ambiguous residues) :: 0 of 47
            Pseudo Genes (frameshifted)       :: 30 of 47
            Pseudo Genes (incomplete)         :: 20 of 47
            Pseudo Genes (internal stop)      :: 9 of 47
            Pseudo Genes (multiple problems)  :: 11 of 47
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..415
                     /organism="Streptococcus pyogenes"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN08555799-rid19682583.denovo.174"
                     /strain="MGAS11113"
                     /serotype="Emm28"
                     /isolation_source="normally sterile site"
                     /host="Homo sapiens"
                     /db_xref="taxon:1314"
                     /country="Finland"
                     /collection_date="2002"
                     /collected_by="National Public Health Laboratory"
     gene            55..>415
                     /gene="grab"
                     /locus_tag="VC122_001794"
     CDS             55..>415
                     /gene="grab"
                     /locus_tag="VC122_001794"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010922409.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="G-related alpha2-macroglobulin-binding protein
                     GRAB"
                     /protein_id="HEP5641676.1"
                     /translation="MGKEIKVKYFLRRSAFGLVAVSASVLVGSTVSAVDSPIEQPRII
                     PNGGTLTNLLGNAPEKLALRNEERAIDELKKQAIEDKEATTAIEAASSDALEALVDQA
                     DALQSEEAAVVQSDTAAS"
BASE COUNT          154 a           69 c           86 g          106 t
ORIGIN      
        1 aaattatcgg taatttaata tgctaatgca tataaaaata aaaaaggaga aacaatggga
       61 aaagaaataa aagtgaaata ctttttgcgt agatcagctt ttggattagt tgcggtgtca
      121 gcatcagtat tagtcggttc aacagtatct gctgttgact cacctatcga acagcctcga
      181 attattccaa atggcggaac cttaactaat cttcttggca atgctccaga aaaactggca
      241 ttacgtaatg aagaaagagc cattgatgaa ttaaaaaaac aagctattga ggataaagaa
      301 gctacgacag ctatagaagc agcaagttca gatgccttag aagcattagt ggatcaagca
      361 gacgctttac aatcagaaga agctgcagta gttcaatcag ataccgctgc tagtg
//
LOCUS       DATXIG010000175          373 bp    DNA     linear   BCT 28-DEC-2023
DEFINITION  TPA_asm: Streptococcus pyogenes strain MGAS11113
            SAMN08555799-rid19682583.denovo.175, whole genome shotgun sequence.
ACCESSION   DATXIG010000175 DATXIG010000000
VERSION     DATXIG010000175.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN08555799
            Sequence Read Archive: SRR6740495
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pyogenes
  ORGANISM  Streptococcus pyogenes
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 373)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 373)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-OCT-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-OCT-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001918058.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001918058.2
            Genome Coverage       :: 368x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/12/2023 11:23:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 1,806
            CDSs (total)                      :: 1,769
            Genes (coding)                    :: 1,722
            CDSs (with protein)               :: 1,722
            Genes (RNA)                       :: 37
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1, 1 (5S, 23S)
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 47
            CDSs (without protein)            :: 47
            Pseudo Genes (ambiguous residues) :: 0 of 47
            Pseudo Genes (frameshifted)       :: 30 of 47
            Pseudo Genes (incomplete)         :: 20 of 47
            Pseudo Genes (internal stop)      :: 9 of 47
            Pseudo Genes (multiple problems)  :: 11 of 47
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..373
                     /organism="Streptococcus pyogenes"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN08555799-rid19682583.denovo.175"
                     /strain="MGAS11113"
                     /serotype="Emm28"
                     /isolation_source="normally sterile site"
                     /host="Homo sapiens"
                     /db_xref="taxon:1314"
                     /country="Finland"
                     /collection_date="2002"
                     /collected_by="National Public Health Laboratory"
     gene            1..200
                     /locus_tag="VC122_001795"
                     /pseudo
     CDS             1..200
                     /locus_tag="VC122_001795"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011017515.1"
                     /note="frameshifted; incomplete; missing N-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
BASE COUNT          146 a           39 c           63 g          125 t
ORIGIN      
        1 cataaaatat ctttatgaaa aagacaaaat aaatatggaa ctcttaatta gtcttatttc
       61 aaaaataagt aacaaactgg tgaaacagga atttataaaa gttttagtct tgtacgagta
      121 tgtaaaaaac tttttaccag cagattgcat ttacttttca ttatctaatt tatatggaac
      181 aggacattat tcaagtatga actataaaat cttcatacga actaagtcag gtgacatatt
      241 tgatattgct gatggtggta gaattgatga tatggtttct aaatttaata aagtaaacgt
      301 attaggagtt tgcatgggaa taggaaccac tgttttatca caagagattg agtatgaaat
      361 tgaagataga ata
//
LOCUS       DATXIG010000176          368 bp    DNA     linear   BCT 28-DEC-2023
DEFINITION  TPA_asm: Streptococcus pyogenes strain MGAS11113
            SAMN08555799-rid19682583.denovo.176, whole genome shotgun sequence.
ACCESSION   DATXIG010000176 DATXIG010000000
VERSION     DATXIG010000176.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN08555799
            Sequence Read Archive: SRR6740495
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pyogenes
  ORGANISM  Streptococcus pyogenes
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 368)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 368)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-OCT-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-OCT-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001918058.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001918058.2
            Genome Coverage       :: 368x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/12/2023 11:23:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 1,806
            CDSs (total)                      :: 1,769
            Genes (coding)                    :: 1,722
            CDSs (with protein)               :: 1,722
            Genes (RNA)                       :: 37
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1, 1 (5S, 23S)
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 47
            CDSs (without protein)            :: 47
            Pseudo Genes (ambiguous residues) :: 0 of 47
            Pseudo Genes (frameshifted)       :: 30 of 47
            Pseudo Genes (incomplete)         :: 20 of 47
            Pseudo Genes (internal stop)      :: 9 of 47
            Pseudo Genes (multiple problems)  :: 11 of 47
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..368
                     /organism="Streptococcus pyogenes"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN08555799-rid19682583.denovo.176"
                     /strain="MGAS11113"
                     /serotype="Emm28"
                     /isolation_source="normally sterile site"
                     /host="Homo sapiens"
                     /db_xref="taxon:1314"
                     /country="Finland"
                     /collection_date="2002"
                     /collected_by="National Public Health Laboratory"
     gene            <1..>368
                     /locus_tag="VC122_001796"
     CDS             <1..>368
                     /locus_tag="VC122_001796"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000572867.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEP5641677.1"
                     /translation="MPLLECGFSFNSKFREYFSVVTGVLPFKFTADMATAWRKVKRQN
                     DLSFTIQDMLKVYYGKSDYAKYDNSVCQWNQFLKDFCADENSCNYSNKIQVAAILWKE
                     VRDSKNKKVYSRKLIKKYED"
BASE COUNT          139 a           42 c           63 g          124 t
ORIGIN      
        1 atatgccttt acttgagtgt ggattttcct ttaattcaaa attcagagaa tatttttctg
       61 ttgtaacagg tgttttgcct tttaaattta ctgcagatat ggcaacggct tggagaaaag
      121 taaaaagaca aaatgatttg agttttacaa ttcaagatat gctcaaagtt tattatggaa
      181 aatcagatta tgctaagtat gataattctg tttgtcaatg gaatcaattt ttaaaggatt
      241 tttgtgcaga cgaaaatagc tgtaactact ctaataaaat acaagtagct gcaattcttt
      301 ggaaagaagt cagagattca aaaaacaaaa aagtttattc acgaaaactt ataaaaaaat
      361 atgaggat
//
LOCUS       DATXIG010000177          360 bp    DNA     linear   BCT 28-DEC-2023
DEFINITION  TPA_asm: Streptococcus pyogenes strain MGAS11113
            SAMN08555799-rid19682583.denovo.177, whole genome shotgun sequence.
ACCESSION   DATXIG010000177 DATXIG010000000
VERSION     DATXIG010000177.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN08555799
            Sequence Read Archive: SRR6740495
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pyogenes
  ORGANISM  Streptococcus pyogenes
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 360)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 360)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-OCT-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-OCT-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001918058.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001918058.2
            Genome Coverage       :: 368x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/12/2023 11:23:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 1,806
            CDSs (total)                      :: 1,769
            Genes (coding)                    :: 1,722
            CDSs (with protein)               :: 1,722
            Genes (RNA)                       :: 37
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1, 1 (5S, 23S)
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 47
            CDSs (without protein)            :: 47
            Pseudo Genes (ambiguous residues) :: 0 of 47
            Pseudo Genes (frameshifted)       :: 30 of 47
            Pseudo Genes (incomplete)         :: 20 of 47
            Pseudo Genes (internal stop)      :: 9 of 47
            Pseudo Genes (multiple problems)  :: 11 of 47
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..360
                     /organism="Streptococcus pyogenes"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN08555799-rid19682583.denovo.177"
                     /strain="MGAS11113"
                     /serotype="Emm28"
                     /isolation_source="normally sterile site"
                     /host="Homo sapiens"
                     /db_xref="taxon:1314"
                     /country="Finland"
                     /collection_date="2002"
                     /collected_by="National Public Health Laboratory"
     gene            complement(<1..>360)
                     /locus_tag="VC122_001797"
     CDS             complement(<1..>360)
                     /locus_tag="VC122_001797"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002989896.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="lanthionine synthetase"
                     /protein_id="HEP5641678.1"
                     /translation="NKVLINIESKYRNTFFDKSLSHGVPSLILMYSSLYKVTKDERYL
                     TFSNIYVEKLVDIISRDGIESPSLYAGTAGIALAIKEASISGKYYPKLLSSLNCLLKE
                     QIKDKLVVSFSNIEKGI"
BASE COUNT          119 a           57 c           48 g          136 t
ORIGIN      
        1 taattccttt ctcaatatta gaaaaactta ctaccaattt gtctttaatt tgttctttta
       61 gaagacaatt taaggatgat aataatttcg gataatactt tccagatata ctcgcttctt
      121 taatagctaa tgcaatacct gcagtgccag catagagaga cggagattct ataccatctc
      181 tagagattat gtctactaat ttctcaacgt atatgttgga gaatgttaga tatctctcat
      241 cttttgtcac tttatatagt gaactataca ttaagattaa tgatggcaca ccatgactca
      301 gagatttatc aaaaaaggta tttctatatt tagattcaat attaattagt actttattct
//
LOCUS       DATXIG010000178          338 bp    DNA     linear   BCT 28-DEC-2023
DEFINITION  TPA_asm: Streptococcus pyogenes strain MGAS11113
            SAMN08555799-rid19682583.denovo.180, whole genome shotgun sequence.
ACCESSION   DATXIG010000178 DATXIG010000000
VERSION     DATXIG010000178.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN08555799
            Sequence Read Archive: SRR6740495
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pyogenes
  ORGANISM  Streptococcus pyogenes
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 338)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 338)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-OCT-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-OCT-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001918058.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001918058.2
            Genome Coverage       :: 368x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/12/2023 11:23:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 1,806
            CDSs (total)                      :: 1,769
            Genes (coding)                    :: 1,722
            CDSs (with protein)               :: 1,722
            Genes (RNA)                       :: 37
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1, 1 (5S, 23S)
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 47
            CDSs (without protein)            :: 47
            Pseudo Genes (ambiguous residues) :: 0 of 47
            Pseudo Genes (frameshifted)       :: 30 of 47
            Pseudo Genes (incomplete)         :: 20 of 47
            Pseudo Genes (internal stop)      :: 9 of 47
            Pseudo Genes (multiple problems)  :: 11 of 47
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..338
                     /organism="Streptococcus pyogenes"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN08555799-rid19682583.denovo.180"
                     /strain="MGAS11113"
                     /serotype="Emm28"
                     /isolation_source="normally sterile site"
                     /host="Homo sapiens"
                     /db_xref="taxon:1314"
                     /country="Finland"
                     /collection_date="2002"
                     /collected_by="National Public Health Laboratory"
     gene            <1..>338
                     /locus_tag="VC122_001798"
     CDS             <1..>338
                     /locus_tag="VC122_001798"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011528611.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="lantibiotic dehydratase"
                     /protein_id="HEP5641679.1"
                     /translation="NEKKKQNVKVSLNSYSKRAAFRTTPFGIFTAINTVDLTKGTTSN
                     VQKVSFIKKAVPDYSWIYSLVKSYEISNLEKLSLKINTAAFTQGDRYVLPFTVNESEE
                     DRNISFSKPI"
BASE COUNT          137 a           47 c           53 g          101 t
ORIGIN      
        1 gtaatgaaaa gaaaaagcaa aatgtaaaag tctcattaaa tagttattct aaaagagcag
       61 cgtttagaac tactccattt ggtatcttta ctgctattaa tacagttgat ttgacaaagg
      121 gaaccacttc taatgtacaa aaggttagtt ttattaaaaa agcagtccca gattattcat
      181 ggatatattc tctagtaaaa tcgtatgaaa taagtaacct tgaaaaactt tcattaaaaa
      241 taaatacagc ggcgtttact caaggtgaca gatatgtact accatttaca gtgaatgaat
      301 cagaggaaga tagaaacata agtttttcaa aaccaatt
//
LOCUS       DATXIG010000179          327 bp    DNA     linear   BCT 28-DEC-2023
DEFINITION  TPA_asm: Streptococcus pyogenes strain MGAS11113
            SAMN08555799-rid19682583.denovo.181, whole genome shotgun sequence.
ACCESSION   DATXIG010000179 DATXIG010000000
VERSION     DATXIG010000179.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN08555799
            Sequence Read Archive: SRR6740495
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pyogenes
  ORGANISM  Streptococcus pyogenes
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 327)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 327)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-OCT-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-OCT-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001918058.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001918058.2
            Genome Coverage       :: 368x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/12/2023 11:23:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 1,806
            CDSs (total)                      :: 1,769
            Genes (coding)                    :: 1,722
            CDSs (with protein)               :: 1,722
            Genes (RNA)                       :: 37
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1, 1 (5S, 23S)
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 47
            CDSs (without protein)            :: 47
            Pseudo Genes (ambiguous residues) :: 0 of 47
            Pseudo Genes (frameshifted)       :: 30 of 47
            Pseudo Genes (incomplete)         :: 20 of 47
            Pseudo Genes (internal stop)      :: 9 of 47
            Pseudo Genes (multiple problems)  :: 11 of 47
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..327
                     /organism="Streptococcus pyogenes"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN08555799-rid19682583.denovo.181"
                     /strain="MGAS11113"
                     /serotype="Emm28"
                     /isolation_source="normally sterile site"
                     /host="Homo sapiens"
                     /db_xref="taxon:1314"
                     /country="Finland"
                     /collection_date="2002"
                     /collected_by="National Public Health Laboratory"
BASE COUNT          132 a           58 c           42 g           95 t
ORIGIN      
        1 taactacttg tcaaacagtc ttgattccaa cttttaaata ttcgtatatg aagtaaagct
       61 actgtgcatg aaacaaaaaa tataacaata agtggaacaa ttgccatttt ctgttggtta
      121 tatagagtat ctgatattct taaaagccat gcccatggaa taagaaacca taccttatca
      181 ccaaggctcg tttcacaaaa agctgcaaca ataacaccta ccattgaaat acctaatgcc
      241 acacctgtac taaatatata gctaataata tgattgatca gataggttac ataggcaaaa
      301 atgatataaa ttaaactatt caaaata
//
LOCUS       DATXIG010000180          309 bp    DNA     linear   BCT 28-DEC-2023
DEFINITION  TPA_asm: Streptococcus pyogenes strain MGAS11113
            SAMN08555799-rid19682583.denovo.182, whole genome shotgun sequence.
ACCESSION   DATXIG010000180 DATXIG010000000
VERSION     DATXIG010000180.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN08555799
            Sequence Read Archive: SRR6740495
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pyogenes
  ORGANISM  Streptococcus pyogenes
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 309)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 309)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-OCT-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-OCT-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001918058.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001918058.2
            Genome Coverage       :: 368x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/12/2023 11:23:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 1,806
            CDSs (total)                      :: 1,769
            Genes (coding)                    :: 1,722
            CDSs (with protein)               :: 1,722
            Genes (RNA)                       :: 37
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1, 1 (5S, 23S)
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 47
            CDSs (without protein)            :: 47
            Pseudo Genes (ambiguous residues) :: 0 of 47
            Pseudo Genes (frameshifted)       :: 30 of 47
            Pseudo Genes (incomplete)         :: 20 of 47
            Pseudo Genes (internal stop)      :: 9 of 47
            Pseudo Genes (multiple problems)  :: 11 of 47
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..309
                     /organism="Streptococcus pyogenes"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN08555799-rid19682583.denovo.182"
                     /strain="MGAS11113"
                     /serotype="Emm28"
                     /isolation_source="normally sterile site"
                     /host="Homo sapiens"
                     /db_xref="taxon:1314"
                     /country="Finland"
                     /collection_date="2002"
                     /collected_by="National Public Health Laboratory"
     gene            33..>309
                     /locus_tag="VC122_001799"
     CDS             33..>309
                     /locus_tag="VC122_001799"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015057919.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific integrase"
                     /protein_id="HEP5641680.1"
                     /translation="MATYRQRGKKKLWDYRVYGKDGKLIASGSGFRTKKEASYEALKI
                     EQKKYYSDVINSNATLFEMWQIWYDLVVEPSELDILTKQKYQARGKII"
BASE COUNT          128 a           32 c           61 g           88 t
ORIGIN      
        1 ataaattcct tgatgtctag gaaaggagaa aaatggcaac atatagacaa agaggtaaaa
       61 agaaattatg ggattatcgt gtttatggta aagatggaaa attaatagca tctggttcag
      121 gctttagaac aaaaaaagaa gcatcatatg aagctttaaa aattgagcaa aaaaaatatt
      181 attcagatgt tataaattcc aatgctacat tatttgagat gtggcaaata tggtatgatc
      241 ttgttgttga gccaagtgaa ttagatattc ttactaagca aaaatatcaa gcccgaggaa
      301 agataattg
//
LOCUS       DATXIG010000181          300 bp    DNA     linear   BCT 28-DEC-2023
DEFINITION  TPA_asm: Streptococcus pyogenes strain MGAS11113
            SAMN08555799-rid19682583.denovo.183, whole genome shotgun sequence.
ACCESSION   DATXIG010000181 DATXIG010000000
VERSION     DATXIG010000181.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN08555799
            Sequence Read Archive: SRR6740495
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pyogenes
  ORGANISM  Streptococcus pyogenes
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 300)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 300)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-OCT-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-OCT-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001918058.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001918058.2
            Genome Coverage       :: 368x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/12/2023 11:23:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 1,806
            CDSs (total)                      :: 1,769
            Genes (coding)                    :: 1,722
            CDSs (with protein)               :: 1,722
            Genes (RNA)                       :: 37
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1, 1 (5S, 23S)
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 47
            CDSs (without protein)            :: 47
            Pseudo Genes (ambiguous residues) :: 0 of 47
            Pseudo Genes (frameshifted)       :: 30 of 47
            Pseudo Genes (incomplete)         :: 20 of 47
            Pseudo Genes (internal stop)      :: 9 of 47
            Pseudo Genes (multiple problems)  :: 11 of 47
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..300
                     /organism="Streptococcus pyogenes"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN08555799-rid19682583.denovo.183"
                     /strain="MGAS11113"
                     /serotype="Emm28"
                     /isolation_source="normally sterile site"
                     /host="Homo sapiens"
                     /db_xref="taxon:1314"
                     /country="Finland"
                     /collection_date="2002"
                     /collected_by="National Public Health Laboratory"
     gene            <1..>300
                     /locus_tag="VC122_001800"
     CDS             <1..>300
                     /locus_tag="VC122_001800"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000490637.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HEP5641681.1"
                     /translation="KLKTLRTEAGLTQKELAKIIQTSQQNIAYWEKGSRNPKHKSIEK
                     LANVFNVSTDYLLGNTDLKNPNDLDIEKVKESLKKSLGYNGTSDIPEDELESMAQA"
BASE COUNT          129 a           40 c           50 g           81 t
ORIGIN      
        1 aaaattaaaa acattacgaa cagaagctgg acttactcaa aaagaattag ctaaaattat
       61 tcaaactagt caacaaaata ttgcatattg ggagaagggt agtagaaatc ccaagcataa
      121 atcaatagaa aaattagcta acgtttttaa cgtttctaca gattatttat tgggaaatac
      181 tgatttaaag aatcctaatg atttagacat tgaaaaagta aaagagagcc taaaaaaatc
      241 tcttggttac aatggaactt ctgatattcc tgaagatgag ctagaaagta tggcacaagc
//
LOCUS       DATXIG010000182          267 bp    DNA     linear   BCT 28-DEC-2023
DEFINITION  TPA_asm: Streptococcus pyogenes strain MGAS11113
            SAMN08555799-rid19682583.denovo.184, whole genome shotgun sequence.
ACCESSION   DATXIG010000182 DATXIG010000000
VERSION     DATXIG010000182.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN08555799
            Sequence Read Archive: SRR6740495
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pyogenes
  ORGANISM  Streptococcus pyogenes
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 267)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 267)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-OCT-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-OCT-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001918058.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001918058.2
            Genome Coverage       :: 368x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/12/2023 11:23:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 1,806
            CDSs (total)                      :: 1,769
            Genes (coding)                    :: 1,722
            CDSs (with protein)               :: 1,722
            Genes (RNA)                       :: 37
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1, 1 (5S, 23S)
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 47
            CDSs (without protein)            :: 47
            Pseudo Genes (ambiguous residues) :: 0 of 47
            Pseudo Genes (frameshifted)       :: 30 of 47
            Pseudo Genes (incomplete)         :: 20 of 47
            Pseudo Genes (internal stop)      :: 9 of 47
            Pseudo Genes (multiple problems)  :: 11 of 47
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..267
                     /organism="Streptococcus pyogenes"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN08555799-rid19682583.denovo.184"
                     /strain="MGAS11113"
                     /serotype="Emm28"
                     /isolation_source="normally sterile site"
                     /host="Homo sapiens"
                     /db_xref="taxon:1314"
                     /country="Finland"
                     /collection_date="2002"
                     /collected_by="National Public Health Laboratory"
BASE COUNT           90 a           47 c           31 g           99 t
ORIGIN      
        1 ttaattgtat tagacaaaat tccgtcccat tctacctctg acagcccatg tggatactgt
       61 ttttcttcct gtaataaaaa caacgctctt aactttaaat tctcgatatc attgctggat
      121 aggatatcac tggacttaat aaactgtctt gaatgacttt caaaatttat atttaaacgt
      181 aacttatctt gattcaaaga attatcatta atagtttcga agaaactttg tgaaacttct
      241 aaaaaaattt tttcaatctt ctcaaag
//
LOCUS       DATXIG010000183          263 bp    DNA     linear   BCT 28-DEC-2023
DEFINITION  TPA_asm: Streptococcus pyogenes strain MGAS11113
            SAMN08555799-rid19682583.denovo.185, whole genome shotgun sequence.
ACCESSION   DATXIG010000183 DATXIG010000000
VERSION     DATXIG010000183.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN08555799
            Sequence Read Archive: SRR6740495
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pyogenes
  ORGANISM  Streptococcus pyogenes
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 263)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 263)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-OCT-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-OCT-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001918058.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001918058.2
            Genome Coverage       :: 368x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/12/2023 11:23:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 1,806
            CDSs (total)                      :: 1,769
            Genes (coding)                    :: 1,722
            CDSs (with protein)               :: 1,722
            Genes (RNA)                       :: 37
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1, 1 (5S, 23S)
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 47
            CDSs (without protein)            :: 47
            Pseudo Genes (ambiguous residues) :: 0 of 47
            Pseudo Genes (frameshifted)       :: 30 of 47
            Pseudo Genes (incomplete)         :: 20 of 47
            Pseudo Genes (internal stop)      :: 9 of 47
            Pseudo Genes (multiple problems)  :: 11 of 47
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..263
                     /organism="Streptococcus pyogenes"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN08555799-rid19682583.denovo.185"
                     /strain="MGAS11113"
                     /serotype="Emm28"
                     /isolation_source="normally sterile site"
                     /host="Homo sapiens"
                     /db_xref="taxon:1314"
                     /country="Finland"
                     /collection_date="2002"
                     /collected_by="National Public Health Laboratory"
     gene            <1..>263
                     /locus_tag="VC122_001801"
     CDS             <1..>263
                     /locus_tag="VC122_001801"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611423.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HEP5641682.1"
                     /translation="KKAERIPLEGRSKAQRATEIIQALRNQFPLEMLLEILDLSRSTY
                     YYQVKRLAQGDKDIELKHVIREIYDEHKGNYGYRRIHMELRNRG"
BASE COUNT           94 a           44 c           59 g           66 t
ORIGIN      
        1 aaaaaagctg agagaatacc gcttgaggga cgaagtaaag ctcaaagagc aacagaaatc
       61 attcaagcat taaggaatca atttccctta gagatgctac ttgagatcct tgatttatca
      121 cgctccacct attactatca agtgaagcga ctagctcaag gagataagga tatagaacta
      181 aagcatgtga ttcgagagat ttatgatgag cataaaggta attacggtta tcgtcgtatt
      241 catatggagt tgcgtaaccg agg
//
LOCUS       DATXIG010000184          248 bp    DNA     linear   BCT 28-DEC-2023
DEFINITION  TPA_asm: Streptococcus pyogenes strain MGAS11113
            SAMN08555799-rid19682583.denovo.186, whole genome shotgun sequence.
ACCESSION   DATXIG010000184 DATXIG010000000
VERSION     DATXIG010000184.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN08555799
            Sequence Read Archive: SRR6740495
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pyogenes
  ORGANISM  Streptococcus pyogenes
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 248)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 248)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-OCT-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-OCT-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001918058.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001918058.2
            Genome Coverage       :: 368x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/12/2023 11:23:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 1,806
            CDSs (total)                      :: 1,769
            Genes (coding)                    :: 1,722
            CDSs (with protein)               :: 1,722
            Genes (RNA)                       :: 37
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1, 1 (5S, 23S)
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 47
            CDSs (without protein)            :: 47
            Pseudo Genes (ambiguous residues) :: 0 of 47
            Pseudo Genes (frameshifted)       :: 30 of 47
            Pseudo Genes (incomplete)         :: 20 of 47
            Pseudo Genes (internal stop)      :: 9 of 47
            Pseudo Genes (multiple problems)  :: 11 of 47
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..248
                     /organism="Streptococcus pyogenes"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN08555799-rid19682583.denovo.186"
                     /strain="MGAS11113"
                     /serotype="Emm28"
                     /isolation_source="normally sterile site"
                     /host="Homo sapiens"
                     /db_xref="taxon:1314"
                     /country="Finland"
                     /collection_date="2002"
                     /collected_by="National Public Health Laboratory"
     gene            154..>248
                     /gene="rrf"
                     /locus_tag="VC122_001802"
     rRNA            154..>248
                     /gene="rrf"
                     /locus_tag="VC122_001802"
                     /product="5S ribosomal RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00001"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00001"
BASE COUNT           78 a           44 c           59 g           67 t
ORIGIN      
        1 taggttagga gtgtaagtgt agcgatacat gtagcggact aatactaata gctcgaggac
       61 ttatccaaaa agaaatattg acaacgttac ggattcttgt tagactatag atattcaatt
      121 ttgagtgggt tatgaaaata gcacatttac aagttaagtg acgatagcct aggagataca
      181 cctgtaccca tgccgaacac agcagttaag ccctagaacg cctgatgtag ttgggggttg
      241 ccccctgt
//
LOCUS       DATXIG010000185          242 bp    DNA     linear   BCT 28-DEC-2023
DEFINITION  TPA_asm: Streptococcus pyogenes strain MGAS11113
            SAMN08555799-rid19682583.denovo.187, whole genome shotgun sequence.
ACCESSION   DATXIG010000185 DATXIG010000000
VERSION     DATXIG010000185.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN08555799
            Sequence Read Archive: SRR6740495
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pyogenes
  ORGANISM  Streptococcus pyogenes
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 242)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 242)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-OCT-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-OCT-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001918058.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001918058.2
            Genome Coverage       :: 368x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/12/2023 11:23:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 1,806
            CDSs (total)                      :: 1,769
            Genes (coding)                    :: 1,722
            CDSs (with protein)               :: 1,722
            Genes (RNA)                       :: 37
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1, 1 (5S, 23S)
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 47
            CDSs (without protein)            :: 47
            Pseudo Genes (ambiguous residues) :: 0 of 47
            Pseudo Genes (frameshifted)       :: 30 of 47
            Pseudo Genes (incomplete)         :: 20 of 47
            Pseudo Genes (internal stop)      :: 9 of 47
            Pseudo Genes (multiple problems)  :: 11 of 47
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..242
                     /organism="Streptococcus pyogenes"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN08555799-rid19682583.denovo.187"
                     /strain="MGAS11113"
                     /serotype="Emm28"
                     /isolation_source="normally sterile site"
                     /host="Homo sapiens"
                     /db_xref="taxon:1314"
                     /country="Finland"
                     /collection_date="2002"
                     /collected_by="National Public Health Laboratory"
BASE COUNT           90 a           36 c           35 g           81 t
ORIGIN      
        1 tagcacaatc tttggcagca atctgaattg tttttccata aaagatttaa gcacttacgt
       61 ttgcttaaaa tataggttat gtgacgtgcg tgcaagatca gattataaca aaatagttcc
      121 aaataagcct atcactgaga aagcatcaca gaagggttaa actttttttg tattagtgtt
      181 ttatcagtac aacctaacac ttatttttat aaaaaaataa aaaagctatt gacaatctta
      241 ta
//
LOCUS       DATXIG010000186          237 bp    DNA     linear   BCT 28-DEC-2023
DEFINITION  TPA_asm: Streptococcus pyogenes strain MGAS11113
            SAMN08555799-rid19682583.denovo.188, whole genome shotgun sequence.
ACCESSION   DATXIG010000186 DATXIG010000000
VERSION     DATXIG010000186.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN08555799
            Sequence Read Archive: SRR6740495
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pyogenes
  ORGANISM  Streptococcus pyogenes
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 237)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 237)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-OCT-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-OCT-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001918058.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001918058.2
            Genome Coverage       :: 368x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/12/2023 11:23:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 1,806
            CDSs (total)                      :: 1,769
            Genes (coding)                    :: 1,722
            CDSs (with protein)               :: 1,722
            Genes (RNA)                       :: 37
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1, 1 (5S, 23S)
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 47
            CDSs (without protein)            :: 47
            Pseudo Genes (ambiguous residues) :: 0 of 47
            Pseudo Genes (frameshifted)       :: 30 of 47
            Pseudo Genes (incomplete)         :: 20 of 47
            Pseudo Genes (internal stop)      :: 9 of 47
            Pseudo Genes (multiple problems)  :: 11 of 47
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..237
                     /organism="Streptococcus pyogenes"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN08555799-rid19682583.denovo.188"
                     /strain="MGAS11113"
                     /serotype="Emm28"
                     /isolation_source="normally sterile site"
                     /host="Homo sapiens"
                     /db_xref="taxon:1314"
                     /country="Finland"
                     /collection_date="2002"
                     /collected_by="National Public Health Laboratory"
     gene            <1..>237
                     /locus_tag="VC122_001803"
     CDS             <1..>237
                     /locus_tag="VC122_001803"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_150136628.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="YSIRK signal domain/LPXTG anchor domain surface
                     protein"
                     /protein_id="HEP5641683.1"
                     /translation="KDTVDVTVKVVDPRTDADKNDPAGKDQTVKVGETPKAEDSIGNL
                     SDLPKGTTVAFEAPVDTATPGDKPAKVVVTYPDGS"
BASE COUNT           82 a           47 c           57 g           51 t
ORIGIN      
        1 aaaagatact gtagatgtga ctgttaaggt tgtcgatcca cgtacagatg ccgataagaa
       61 tgatccagca ggtaaggacc aaacggtcaa agtaggagaa acaccgaagg cagaagattc
      121 tattggtaac ttatcagatc ttccgaaagg tacaacagta gcctttgaag ctccagttga
      181 tacagcaaca ccgggagaca aaccagcaaa agttgttgtg acttacccag atggttc
//
LOCUS       DATXIG010000187          226 bp    DNA     linear   BCT 28-DEC-2023
DEFINITION  TPA_asm: Streptococcus pyogenes strain MGAS11113
            SAMN08555799-rid19682583.denovo.190, whole genome shotgun sequence.
ACCESSION   DATXIG010000187 DATXIG010000000
VERSION     DATXIG010000187.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN08555799
            Sequence Read Archive: SRR6740495
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pyogenes
  ORGANISM  Streptococcus pyogenes
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 226)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 226)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-OCT-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-OCT-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001918058.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001918058.2
            Genome Coverage       :: 368x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/12/2023 11:23:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 1,806
            CDSs (total)                      :: 1,769
            Genes (coding)                    :: 1,722
            CDSs (with protein)               :: 1,722
            Genes (RNA)                       :: 37
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1, 1 (5S, 23S)
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 47
            CDSs (without protein)            :: 47
            Pseudo Genes (ambiguous residues) :: 0 of 47
            Pseudo Genes (frameshifted)       :: 30 of 47
            Pseudo Genes (incomplete)         :: 20 of 47
            Pseudo Genes (internal stop)      :: 9 of 47
            Pseudo Genes (multiple problems)  :: 11 of 47
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..226
                     /organism="Streptococcus pyogenes"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN08555799-rid19682583.denovo.190"
                     /strain="MGAS11113"
                     /serotype="Emm28"
                     /isolation_source="normally sterile site"
                     /host="Homo sapiens"
                     /db_xref="taxon:1314"
                     /country="Finland"
                     /collection_date="2002"
                     /collected_by="National Public Health Laboratory"
BASE COUNT           78 a           35 c           32 g           81 t
ORIGIN      
        1 ttagtttgct cctttattag tagataagga agttatacat gattaaagct cagctaaaga
       61 atctaataaa aacaattcat ctcgtaaaag aggctggttt ctccctactg gtgttctcaa
      121 ttgctattag cttatttcaa gggattttac ccatttttag tatgctacta gttcaaaaaa
      181 tgttaaacat tataacgact gatataaaaa attttcacac attgat
//
LOCUS       DATXIG010000188          226 bp    DNA     linear   BCT 28-DEC-2023
DEFINITION  TPA_asm: Streptococcus pyogenes strain MGAS11113
            SAMN08555799-rid19682583.denovo.191, whole genome shotgun sequence.
ACCESSION   DATXIG010000188 DATXIG010000000
VERSION     DATXIG010000188.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN08555799
            Sequence Read Archive: SRR6740495
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pyogenes
  ORGANISM  Streptococcus pyogenes
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 226)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 226)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-OCT-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-OCT-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001918058.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001918058.2
            Genome Coverage       :: 368x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/12/2023 11:23:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 1,806
            CDSs (total)                      :: 1,769
            Genes (coding)                    :: 1,722
            CDSs (with protein)               :: 1,722
            Genes (RNA)                       :: 37
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1, 1 (5S, 23S)
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 47
            CDSs (without protein)            :: 47
            Pseudo Genes (ambiguous residues) :: 0 of 47
            Pseudo Genes (frameshifted)       :: 30 of 47
            Pseudo Genes (incomplete)         :: 20 of 47
            Pseudo Genes (internal stop)      :: 9 of 47
            Pseudo Genes (multiple problems)  :: 11 of 47
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..226
                     /organism="Streptococcus pyogenes"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN08555799-rid19682583.denovo.191"
                     /strain="MGAS11113"
                     /serotype="Emm28"
                     /isolation_source="normally sterile site"
                     /host="Homo sapiens"
                     /db_xref="taxon:1314"
                     /country="Finland"
                     /collection_date="2002"
                     /collected_by="National Public Health Laboratory"
BASE COUNT           66 a           32 c           36 g           92 t
ORIGIN      
        1 ttatttccct tacttctatt tttctattgg agtcaacgtg gaggtttaca aagcaggact
       61 gtattatttt cctactttca aatagtaggt gtacttcttc cttttgttgc aagtattgtg
      121 tgcattcaac taaaaaattt ggaagaatca tctggaaaat ataaatattt attgggttac
      181 tcacagtcga attataagcc atttattgta gaattagtat ttctat
//
LOCUS       DATXIG010000189          224 bp    DNA     linear   BCT 28-DEC-2023
DEFINITION  TPA_asm: Streptococcus pyogenes strain MGAS11113
            SAMN08555799-rid19682583.denovo.192, whole genome shotgun sequence.
ACCESSION   DATXIG010000189 DATXIG010000000
VERSION     DATXIG010000189.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN08555799
            Sequence Read Archive: SRR6740495
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pyogenes
  ORGANISM  Streptococcus pyogenes
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 224)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 224)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-OCT-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-OCT-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001918058.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001918058.2
            Genome Coverage       :: 368x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/12/2023 11:23:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 1,806
            CDSs (total)                      :: 1,769
            Genes (coding)                    :: 1,722
            CDSs (with protein)               :: 1,722
            Genes (RNA)                       :: 37
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1, 1 (5S, 23S)
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 47
            CDSs (without protein)            :: 47
            Pseudo Genes (ambiguous residues) :: 0 of 47
            Pseudo Genes (frameshifted)       :: 30 of 47
            Pseudo Genes (incomplete)         :: 20 of 47
            Pseudo Genes (internal stop)      :: 9 of 47
            Pseudo Genes (multiple problems)  :: 11 of 47
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..224
                     /organism="Streptococcus pyogenes"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN08555799-rid19682583.denovo.192"
                     /strain="MGAS11113"
                     /serotype="Emm28"
                     /isolation_source="normally sterile site"
                     /host="Homo sapiens"
                     /db_xref="taxon:1314"
                     /country="Finland"
                     /collection_date="2002"
                     /collected_by="National Public Health Laboratory"
     gene            4..>224
                     /locus_tag="VC122_001804"
     CDS             4..>224
                     /locus_tag="VC122_001804"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002989251.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="deacetylase"
                     /protein_id="HEP5641684.1"
                     /translation="MKKLNLILVGLLSILILSLAIVFINRWKLNEDSQRIVLAEKKKN
                     TSDLVIKAVKHIKKDQKDYYYFSPIKQAD"
BASE COUNT           87 a           26 c           36 g           75 t
ORIGIN      
        1 ctattgaaaa aattaaatct tattcttgtt ggtttattaa gcattctgat tttgagttta
       61 gctattgtgt ttattaatcg ttggaaacta aacgaagata gtcagcgtat agttttggct
      121 gaaaagaaaa aaaacacgtc agatttagtg atcaaagctg taaaacatat taaaaaagat
      181 caaaaagact attattattt ttccccgata aaacaagcag atga
//
LOCUS       DATXIG010000190          221 bp    DNA     linear   BCT 28-DEC-2023
DEFINITION  TPA_asm: Streptococcus pyogenes strain MGAS11113
            SAMN08555799-rid19682583.denovo.193, whole genome shotgun sequence.
ACCESSION   DATXIG010000190 DATXIG010000000
VERSION     DATXIG010000190.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN08555799
            Sequence Read Archive: SRR6740495
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pyogenes
  ORGANISM  Streptococcus pyogenes
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 221)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 221)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-OCT-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-OCT-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001918058.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001918058.2
            Genome Coverage       :: 368x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/12/2023 11:23:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 1,806
            CDSs (total)                      :: 1,769
            Genes (coding)                    :: 1,722
            CDSs (with protein)               :: 1,722
            Genes (RNA)                       :: 37
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1, 1 (5S, 23S)
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 47
            CDSs (without protein)            :: 47
            Pseudo Genes (ambiguous residues) :: 0 of 47
            Pseudo Genes (frameshifted)       :: 30 of 47
            Pseudo Genes (incomplete)         :: 20 of 47
            Pseudo Genes (internal stop)      :: 9 of 47
            Pseudo Genes (multiple problems)  :: 11 of 47
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..221
                     /organism="Streptococcus pyogenes"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN08555799-rid19682583.denovo.193"
                     /strain="MGAS11113"
                     /serotype="Emm28"
                     /isolation_source="normally sterile site"
                     /host="Homo sapiens"
                     /db_xref="taxon:1314"
                     /country="Finland"
                     /collection_date="2002"
                     /collected_by="National Public Health Laboratory"
     gene            <1..>221
                     /locus_tag="VC122_001805"
     CDS             <1..>221
                     /locus_tag="VC122_001805"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002990440.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1542 domain-containing protein"
                     /protein_id="HEP5641685.1"
                     /translation="KTGDPVVARREAHGKQLDRVAQETKDAIEKDPTLTTEEKAKQVK
                     DVDAAKERGMAKLTEAKNADELDKAYGEGV"
BASE COUNT           79 a           38 c           58 g           46 t
ORIGIN      
        1 aagacaggtg accctgtcgt agctcgtcgt gaggctcatg gtaagcaact tgatagagtc
       61 gctcaagaaa ctaaagatgc gattgaaaaa gaccctactt tgacgactga agaaaaagct
      121 aaacaagtta aagacgtaga tgccgctaaa gaaagaggca tggctaagct tactgaagct
      181 aagaatgctg atgagttaga caaagcttac ggtgaaggtg t
//
LOCUS       DATXIG010000191          213 bp    DNA     linear   BCT 28-DEC-2023
DEFINITION  TPA_asm: Streptococcus pyogenes strain MGAS11113
            SAMN08555799-rid19682583.denovo.194, whole genome shotgun sequence.
ACCESSION   DATXIG010000191 DATXIG010000000
VERSION     DATXIG010000191.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN08555799
            Sequence Read Archive: SRR6740495
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pyogenes
  ORGANISM  Streptococcus pyogenes
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 213)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 213)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-OCT-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-OCT-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001918058.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001918058.2
            Genome Coverage       :: 368x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/12/2023 11:23:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 1,806
            CDSs (total)                      :: 1,769
            Genes (coding)                    :: 1,722
            CDSs (with protein)               :: 1,722
            Genes (RNA)                       :: 37
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1, 1 (5S, 23S)
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 47
            CDSs (without protein)            :: 47
            Pseudo Genes (ambiguous residues) :: 0 of 47
            Pseudo Genes (frameshifted)       :: 30 of 47
            Pseudo Genes (incomplete)         :: 20 of 47
            Pseudo Genes (internal stop)      :: 9 of 47
            Pseudo Genes (multiple problems)  :: 11 of 47
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..213
                     /organism="Streptococcus pyogenes"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN08555799-rid19682583.denovo.194"
                     /strain="MGAS11113"
                     /serotype="Emm28"
                     /isolation_source="normally sterile site"
                     /host="Homo sapiens"
                     /db_xref="taxon:1314"
                     /country="Finland"
                     /collection_date="2002"
                     /collected_by="National Public Health Laboratory"
     gene            <1..>213
                     /locus_tag="VC122_001806"
     CDS             <1..>213
                     /locus_tag="VC122_001806"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000564846.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="ISAs1 family transposase"
                     /protein_id="HEP5641686.1"
                     /translation="VRGHWQIESMHWLLDVVYHEDHHQTLDKRAAFNLNFIRKMCLYF
                     LKVMVFPKKDLSYRRKQRYISVHLED"
BASE COUNT           68 a           34 c           42 g           69 t
ORIGIN      
        1 tgtacgaggt cattggcaga tagagagtat gcactggtta ttggacgttg tttatcatga
       61 agatcatcat cagacattgg ataaaagagc cgcatttaac ctaaatttta tccgaaaaat
      121 gtgcttatat tttctcaaag tgatggtatt tcctaaaaaa gacctcagtt atcgtcgcaa
      181 acaacggtat atttctgtcc atttggaaga tta
//
